title: New analytical application of antibody-based biosensor in estimation of thyroid-stimulating hormone in serum.
abstract: BACKGROUND
  Conventionally, ELISA is used to measure thyroid-stimulating hormone (TSH) for diagnosis of thyroid disease. In this study, an immunosensor-based, kinetic-exclusion analysis (KinExA) was used for TSH estimation.
  
  
  METHODS
  A PMMA microbead column coated with TSH antigen is formed inside the flow cell. Samples consisting of mouse anti-TSH monoclonal antibody and TSH antigen complex in solution are passed over the beads and the unbound anti-TSH antibody is captured by the TSH-coated beads, followed by passing fluorescent-labeled antibody over the beads to generate signals for analysis. The limit of detection for the assay was 0.4 mIU l(-1) and the precision was acceptable.
  
  
  CONCLUSIONS
  The developed sensor was advantageous due to the automated nature and its convenience, without compromising the sensitivity for estimation of TSH.
authors.affiliation: Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi Arabia.
authors.forename: Tanveer A
authors.initials: TA
authors.lastname: Wani
authors.affiliation: Department of Biochemistry, College of Science, King Saud University, PO Box 22452, Riyadh 11451, Saudi Arabia.
authors.forename: Seema
authors.initials: S
authors.lastname: Zargar
authors.affiliation: Department of Genetics, King Faisal Specialist Hospital & Research Centre, Riyadh 11211, Saudi Arabia.
authors.forename: Salma Majid
authors.initials: SM
authors.lastname: Wakil
authors.affiliation: Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi Arabia.
authors.forename: Ibrahim A
authors.initials: IA
authors.lastname: Darwish
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D015374:Biosensing Techniques / Q000592:standards; D002138:Calibration; D005456:Fluorescent Dyes / Q000737:chemistry; D007700:Kinetics; D057230:Limit of Detection; D051379:Mice; D019904:Polymethyl Methacrylate / Q000737:chemistry; D013972:Thyrotropin / Q000097:blood* / Q000276:immunology / Q000592:standards
chemical_list: D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D013972:Thyrotropin; D019904:Polymethyl Methacrylate
keywords: ELISA; TSH; biosensor; thyroid disease