title: Myomir dysregulation and reactive oxygen species in aged human satellite cells.
abstract: Satellite cells that reside on the myofibre surface are crucial for the muscle homeostasis and regeneration. Aging goes along with a less effective regeneration of skeletal muscle tissue mainly due to the decreased myogenic capability of satellite cells. This phenomenon impedes proper maintenance and contributes to the age-associated decline in muscle mass, known as sarcopenia. The myogenic potential impairment does not depend on a reduced myogenic cell number, but mainly on their difficulty to complete a differentiation program. The unbalanced production of reactive oxygen species in elderly people could be responsible for skeletal muscle impairments. microRNAs are conserved post-transcriptional regulators implicated in numerous biological processes including adult myogenesis. Here, we measure the ROS level and analyze myomiR (miR-1, miR-133b and miR-206) expression in human myogenic precursors obtained from Vastus lateralis of elderly and young subjects to provide the molecular signature responsible for the differentiation impairment of elderly activated satellite cells.
authors.affiliation: Interuniversity Institute of Myology (IIM), Department of Neuroscience Imaging and Clinical Sciences, University "G. d'Annunzio" Chieti-Pescara, Chieti, Italy; Center for Excellence on Ageing (CeSI), "G. d'Annunzio" Foundation, Chieti, Italy. Electronic address: es.difilippo@unich.it.
authors.forename: Ester Sara
authors.initials: ES
authors.lastname: Di Filippo
authors.affiliation: Interuniversity Institute of Myology (IIM), Department of Neuroscience Imaging and Clinical Sciences, University "G. d'Annunzio" Chieti-Pescara, Chieti, Italy; Center for Excellence on Ageing (CeSI), "G. d'Annunzio" Foundation, Chieti, Italy. Electronic address: r.mancinelli@unich.it.
authors.forename: Rosa
authors.initials: R
authors.lastname: Mancinelli
authors.affiliation: Interuniversity Institute of Myology (IIM), Department of Neuroscience Imaging and Clinical Sciences, University "G. d'Annunzio" Chieti-Pescara, Chieti, Italy; Center for Excellence on Ageing (CeSI), "G. d'Annunzio" Foundation, Chieti, Italy. Electronic address: tiziana@unich.it.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Pietrangelo
authors.affiliation: Interuniversity Institute of Myology (IIM), Department of Neuroscience Imaging and Clinical Sciences, University "G. d'Annunzio" Chieti-Pescara, Chieti, Italy; Center for Excellence on Ageing (CeSI), "G. d'Annunzio" Foundation, Chieti, Italy. Electronic address: ritalarovere@unich.it.
authors.forename: Rita Maria Laura
authors.initials: RM
authors.lastname: La Rovere
authors.affiliation: Translational Cardiomyology Laboratory, Stem Cell Biology and Embryology Unit, Department of Development and Regeneration, KU Leuven, Leuven, Belgium. Electronic address: mattia.quattrocelli@med.kuleuven.be.
authors.forename: Mattia
authors.initials: M
authors.lastname: Quattrocelli
authors.affiliation: Translational Cardiomyology Laboratory, Stem Cell Biology and Embryology Unit, Department of Development and Regeneration, KU Leuven, Leuven, Belgium; Division of Human Anatomy, Department of Public Health, Experimental and Forensic Medicine, University of Pavia, Pavia, Italy. Electronic address: sampa@unipv.it.
authors.forename: Maurilio
authors.initials: M
authors.lastname: Sampaolesi
authors.affiliation: Interuniversity Institute of Myology (IIM), Department of Neuroscience Imaging and Clinical Sciences, University "G. d'Annunzio" Chieti-Pescara, Chieti, Italy; Center for Excellence on Ageing (CeSI), "G. d'Annunzio" Foundation, Chieti, Italy. Electronic address: sfulle@unich.it.
authors.forename: Stefania
authors.initials: S
authors.lastname: Fulle
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D024510:Muscle Development; D017382:Reactive Oxygen Species / Q000378:metabolism*; D055948:Sarcopenia / Q000235:genetics / Q000378:metabolism; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000378:metabolism*; D055815:Young Adult
chemical_list: D035683:MicroRNAs; D017382:Reactive Oxygen Species
keywords: Aging; Human satellite cells; ROS; Sarcopenia; myomiRNAs