title: Low-level laser therapy attenuates the acute inflammatory response induced by muscle traumatic injury.
abstract: The purpose of this work was to investigate the effect of early and long-term low-level laser therapy (LLLT) on oxidative stress and inflammatory biomarkers after acute-traumatic muscle injury in Wistar rats. Animals were randomly divided into the following four groups: control group (CG), muscle injury group (IG), CG + LLLT, and IG + LLLT: laser treatment with doses of 3 and 5 J/cm(2). Muscle traumatic injury was induced by a single-impact blunt trauma in the rat gastrocnemius. Irradiation for 3 or 5 J/cm(2) was initiated 2, 12, and 24 h after muscle trauma induction, and the treatment was continued for five consecutive days. All the oxidant markers investigated. namely thiobarbituric acid-reactive substance, carbonyl, superoxide dismutase, glutathione peroxidase, and catalase, were increased as soon as 2 h after muscle injury and remained increased up to 24 h. These alterations were prevented by LLLT at a 3 J/cm(2) dose given 2 h after the trauma. Similarly, LLLT prevented the trauma-induced proinflammatory state characterized by IL-6 and IL-10. In parallel, trauma-induced reduction in BDNF and VEGF, vascular remodeling and fiber-proliferating markers, was prevented by laser irradiation. In order to test whether the preventive effect of LLLT was also reflected in muscle functionality, we tested the locomotor activity, by measuring distance traveled and the number of rearings in the open field test. LLLT was effective in recovering the normal locomotion, indicating that the irradiation induced biostimulatory effects that accelerated or resolved the acute inflammatory response as well as the oxidant state elicited by the muscle trauma.
authors.affiliation: a Laboratório de Bioenergética e Estresse Oxidativo - LABOX, Departamento de Bioquímica , Universidade Federal de Santa Catarina , Florianópolis , Brazil ;|b Laboratório de Fisiologia e Bioquímica do Exercício - LaFiBe , Universidade do Extremo Sul Catarinense , Criciúma , Brazil.
authors.forename: Paulo Cesar Lock
authors.initials: PC
authors.lastname: Silveira
authors.affiliation: a Laboratório de Bioenergética e Estresse Oxidativo - LABOX, Departamento de Bioquímica , Universidade Federal de Santa Catarina , Florianópolis , Brazil ;
authors.forename: Debora da Luz
authors.initials: Dda L
authors.lastname: Scheffer
authors.affiliation: a Laboratório de Bioenergética e Estresse Oxidativo - LABOX, Departamento de Bioquímica , Universidade Federal de Santa Catarina , Florianópolis , Brazil ;
authors.forename: Viviane
authors.initials: V
authors.lastname: Glaser
authors.affiliation: a Laboratório de Bioenergética e Estresse Oxidativo - LABOX, Departamento de Bioquímica , Universidade Federal de Santa Catarina , Florianópolis , Brazil ;
authors.forename: Aline Pertile
authors.initials: AP
authors.lastname: Remor
authors.affiliation: b Laboratório de Fisiologia e Bioquímica do Exercício - LaFiBe , Universidade do Extremo Sul Catarinense , Criciúma , Brazil.
authors.forename: Ricardo Aurino
authors.initials: RA
authors.lastname: Pinho
authors.affiliation: a Laboratório de Bioenergética e Estresse Oxidativo - LABOX, Departamento de Bioquímica , Universidade Federal de Santa Catarina , Florianópolis , Brazil ;
authors.forename: Aderbal Silva
authors.initials: AS
authors.lastname: Aguiar Junior
authors.affiliation: a Laboratório de Bioenergética e Estresse Oxidativo - LABOX, Departamento de Bioquímica , Universidade Federal de Santa Catarina , Florianópolis , Brazil ;
authors.forename: Alexandra
authors.initials: A
authors.lastname: Latini
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000378:metabolism* / Q000503:physiopathology; D016753:Interleukin-10 / Q000378:metabolism; D028022:Low-Level Light Therapy; D018482:Muscle, Skeletal / Q000293:injuries / Q000378:metabolism* / Q000503:physiopathology / Q000528:radiation effects; D018384:Oxidative Stress / Q000528:radiation effects*; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing / Q000528:radiation effects
chemical_list: D015415:Biomarkers; D019208:Brain-Derived Neurotrophic Factor; C508609:IL10 protein, human; D017392:Thiobarbituric Acid Reactive Substances; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; D016753:Interleukin-10; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Growth factors; inflammation; low-level laser therapy; muscle trauma; oxidative stress