title: Non-specific phospholipase C1 affects silicon distribution and mechanical strength in stem nodes of rice.
abstract: Silicon, the second abundant element in the crust, is beneficial for plant growth, mechanical strength, and stress responses. Here we show that manipulation of the non-specific phospholipase C1, NPC1, alters silicon content in nodes and husks of rice (Oryza sativa). Silicon content in NPC1-overexpressing (OE) plants was decreased in nodes but increased in husks compared to wild-type, whereas RNAi suppression of NPC1 resulted in the opposite changes to those of NPC1-OE plants. NPC1 from rice hydrolyzed phospholipids and galactolipids to generate diacylglycerol that can be phosphorylated to phosphatidic acid. Phosphatidic acid interacts with Lsi6, a silicon transporter that is expressed at the highest level in nodes. In addition, the node cells of NPC1-OE plants have lower contents of cellulose and hemicellulose, and thinner sclerenchyma and vascular bundle fibre cells than wild-type plants; whereas NPC1-RNAi plants displayed the opposite changes. These data indicate that NPC1 modulates silicon distribution and secondary cell wall deposition in nodes and grains, affecting mechanical strength and seed shattering.
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Huasheng
authors.initials: H
authors.lastname: Cao
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhuo
authors.affiliation: Department of Biology, University of Missouri, St. Louis, Missouri, 63121, USA.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Su
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Linxiao
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Biology, University of Missouri, St. Louis, Missouri, 63121, USA.|Donald Danforth Plant Science Center, St. Louis, Missouri, 63132, USA.
authors.forename: Xuemin
authors.identifier: 0000-0002-6251-6745
authors.initials: X
authors.lastname: Wang
mesh_terms: D001692:Biological Transport; D018506:Gene Expression Regulation, Plant; D012275:Oryza / Q000201:enzymology* / Q000378:metabolism; D010940:Plant Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018547:Plant Stems / Q000201:enzymology / Q000378:metabolism; D034622:RNA Interference; D012825:Silicon / Q000378:metabolism*; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D010940:Plant Proteins; D010738:Type C Phospholipases; C432456:phospholipase C1; D012825:Silicon
keywords: non-specific phospholipase C; phosphatidic acid; secondary cell wall; seed shattering; silicon distribution