title: DNA conformational behavior and compaction in biomimetic systems: Toward better understanding of DNA packaging in cell.
abstract: In a living cell, long genomic DNA is strongly compacted and exists in the environment characterized by a dense macromolecular crowding, high concentrations of mono- and divalent cations, and confinement of ca. 10μm size surrounded by a phospholipid membrane. Experimental modelling of such complex biological system is challenging but important to understand spatiotemporal dynamics and functions of the DNA in cell. The accumulated knowledge about DNA condensation/compaction in conditions resembling those in the real cell can be eventually used to design and construct partly functional "artificial cells" having potential applications in drug delivery systems, gene therapy, and production of synthetic cells. In this review, I would like to overview the past progress in our understanding of the DNA conformational behavior and, in particular, DNA condensation/compaction phenomenon and its relation to the DNA biological activity. This understanding was gained by designing relevant experimental models mimicking DNA behavior in the environment of living cell. Starting with a brief summary of classic experimental systems to study DNA condensation/compaction, in later parts, I highlight recent experimental methodologies to address the effects of macromolecular crowding and nanoscale and microscale confinements on DNA conformation dynamics. All the studies are discussed in the light of their relevance to DNA behavior in living cells, and future prospects of the field are outlined.
authors.affiliation: Graduate School of Environmental Studies, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan. Electronic address: zinchenko@urban.env.nagoya-u.ac.jp.
authors.forename: Anatoly
authors.initials: A
authors.lastname: Zinchenko
mesh_terms: D000818:Animals; D032701:Biomimetics; D004247:DNA; D042003:DNA Packaging; D006801:Humans
chemical_list: D004247:DNA
keywords: Artificial cell; Confinement; Crowding; DNA condensation and compaction; Single-molecule