title: Arthritis-induced alveolar bone loss is associated with changes in the composition of oral microbiota.
abstract: Rheumatoid arthritis (RA) and periodontitis (PD) are chronic inflammatory disorders that cause bone loss. PD tends to be more prevalent and severe in RA patients. Previous experimental studies demonstrated that RA triggers alveolar bone loss similarly to PD. The aim of this study was to investigate if arthritis-induced alveolar bone loss is associated with modification in the oral microbiota. Checkerboard DNA-DNA hybridization was employed to analyze forty oral bacterial species in 3 groups of C57BL/6 mice: control (n = 12; without any challenge); Y4 (n = 8; received oral inoculation of Aggregatibacter Actinomycetemcomitans strain FDC Y4) and AIA group (n = 12; chronic antigen-induced arthritis). The results showed that AIA and Y4 group exhibited similar patterns of bone loss. The AIA group exhibited higher counts of most bacterial species analyzed with predominance of Gram-negative species similarly to infection-induced PD. Prevotella nigrescens and Treponema denticola were detected only in the Y4 group whereas Campylobacter showae, Streptococcus mitis and Streptococcus oralis were only found in the AIA group. Counts of Parvimonas micra, Selenomonas Noxia and Veillonella parvula were greater in the AIA group whereas Actinomyces viscosus and Neisseira mucosa were in large proportion in Y4 group. In conclusion, AIA is associated with changes in the composition of the oral microbiota, which might account for the alveolar bone loss observed in AIA mice.
authors.affiliation: Department of Oral Surgery and Pathology, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
authors.forename: Jôice Dias
authors.initials: JD
authors.lastname: Corrêa
authors.affiliation: Department of Oral Surgery and Pathology, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
authors.forename: Adriana Machado
authors.initials: AM
authors.lastname: Saraiva
authors.affiliation: Department of Morphology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
authors.forename: Celso Martins
authors.initials: CM
authors.lastname: Queiroz-Junior
authors.affiliation: Department of Microbiology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
authors.forename: Mila Fernandes Moreira
authors.initials: MF
authors.lastname: Madeira
authors.affiliation: Department of Periodontology, Faculdade de Odontologia, Universidade de Guarulhos, São Paulo, Brazil.
authors.forename: Poliana Mendes
authors.initials: PM
authors.lastname: Duarte
authors.affiliation: Department of Biochemistry and Immunology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
authors.forename: Mauro Martins
authors.initials: MM
authors.lastname: Teixeira
authors.affiliation: Department of Microbiology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
authors.forename: Danielle Glória
authors.initials: DG
authors.lastname: Souza
authors.affiliation: Department of Oral Surgery and Pathology, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Minas Gerais, Brazil. Electronic address: silva.tarcilia@gmail.com.
authors.forename: Tarcília Aparecida
authors.initials: TA
authors.lastname: da Silva
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016301:Alveolar Bone Loss / Q000382:microbiology* / Q000473:pathology; D000539:Alveolar Process / Q000382:microbiology* / Q000473:pathology; D000818:Animals; D001169:Arthritis, Experimental / Q000382:microbiology* / Q000473:pathology; D002167:Campylobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D008297:Male; D008437:Maxilla / Q000382:microbiology* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D064307:Microbiota / Q000235:genetics*; D009055:Mouth / Q000382:microbiology / Q000473:pathology; D010518:Periodontitis / Q000382:microbiology* / Q000473:pathology; D045242:Prevotella nigrescens / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D034361:Streptococcus mitis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D019533:Streptococcus oralis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D048373:Treponema denticola / Q000145:classification / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial
keywords: Arthritis; Microbiota; Periodontal disease