title: De novo biosynthesis of resveratrol by site-specific integration of heterologous genes in Escherichia coli.
abstract: Resveratrol is a well-known triphenolic natural product present in red wine. For its contribution to human health, the demand for resveratrol as a food and nutrition supplement has increased significantly. In recent years, the rapid development of synthetic biology has promoted extensive work to increase the production of resveratrol in microbes. However, supplementation of expensive phenylpropanoic precursors was required in current engineered strains. Here, we first utilized the site-specific integration strategy to produce resveratrol in Escherichia coli The genes tal, 4cl and sts were site-specific integrated into the loci of genes tyrR and trpED in the chromosome of E. coli BW25113 (DE3). The final strain was capable of producing 4.612 mg L(-1) of resveratrol from glucose.
authors.affiliation: State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, No. 285, Gebaini Road, Shanghai 201203, China.
authors.forename: Xianglei
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, No. 285, Gebaini Road, Shanghai 201203, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lin
authors.affiliation: State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, No. 285, Gebaini Road, Shanghai 201203, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Hu
authors.affiliation: State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, No. 285, Gebaini Road, Shanghai 201203, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, No. 285, Gebaini Road, Shanghai 201203, China Baoquanzhu@126.com.
authors.forename: Baoquan
authors.initials: B
authors.lastname: Zhu
mesh_terms: D000217:Acyltransferases / Q000235:genetics; D000642:Ammonia-Lyases / Q000235:genetics; D003066:Coenzyme A Ligases / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005818:Genetic Engineering / Q000379:methods*; D005947:Glucose / Q000378:metabolism; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism*; D014920:Wine
chemical_list: D013267:Stilbenes; D000217:Acyltransferases; C022674:stilbene synthase; D000642:Ammonia-Lyases; C023208:L-tyrosine ammonia-lyase; D003066:Coenzyme A Ligases; D005947:Glucose; D000077185:Resveratrol
keywords: Escherichia coli; de novo biosynthesis; resveratrol; site-specific integration