title: Comparison between cytospin and liquid-based cytology in urine specimens classified according to the Paris System for Reporting Urinary Cytology.
abstract: BACKGROUND
  The current study compared ThinPrep urinary cytology and conventional cytospin urinary cytology in the diagnosis of bladder cancer, applying the Paris System for Reporting Urinary Cytology.
  
  
  METHODS
  Between January 2010 and December 2011, a total of 3659 urine samples were processed using conventional cytospin methods. Between January 2012 and December 2013, a total of 4186 urine cytological cases were analyzed using ThinPrep methods. In 131 cases (65 processed by conventional cytospin and 66 processed by ThinPrep), a subsequent biopsy was performed. The authors reclassified these cases according to the Paris System and an analysis between the 2 methods with regard to bladder biopsies was performed.
  
  
  RESULTS
  No significant differences were observed in terms of sensitivity and specificity between the 2 methods in cases with positive cytology for high-grade carcinoma. According to the Paris System, cases of atypical urothelial cells (AUC) and atypical urothelial cells suspicious for high-grade carcinoma (AUC-H) that were processed using cytospin did not correlate with urothelial carcinoma or with negative biopsies; conversely, the AUC cases processed using ThinPrep appeared to correlate with negative histological biopsies or low-grade urothelial carcinoma.
  
  
  CONCLUSIONS
  The results of the current study demonstrated that according to the Paris System, there were no significant differences in sensitivity or specificity for the diagnosis of high-grade urothelial carcinoma or AUC-H between the 2 methods. Cases of AUC should be easy to recognize using Thin Prep rather than cytospin and only AUCs diagnosed with ThinPrep were found to be statistically linked to negative cases for carcinoma or with low-grade urothelial carcinoma. Cancer Cytopathol 2016;124:519-23. © 2016 American Cancer Society.
authors.affiliation: Division of Anatomic Pathology and Histology, "Agostino Gemelli" School of Medicine, Catholic University, Rome, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Straccia
authors.affiliation: Division of Anatomic Pathology and Histology, "Agostino Gemelli" School of Medicine, Catholic University, Rome, Italy.
authors.forename: Tommaso
authors.initials: T
authors.lastname: Bizzarro
authors.affiliation: Division of Anatomic Pathology and Histology, "Agostino Gemelli" School of Medicine, Catholic University, Rome, Italy.
authors.forename: Guido
authors.initials: G
authors.lastname: Fadda
authors.affiliation: Division of Anatomic Pathology and Histology, "Agostino Gemelli" School of Medicine, Catholic University, Rome, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Pierconti
mesh_terms: D001706:Biopsy; D003581:Cytodiagnosis / Q000379:methods*; D004847:Epithelial Cells / Q000473:pathology*; D006801:Humans; D014556:Urine / Q000166:cytology*; D014571:Urologic Neoplasms / Q000145:classification / Q000175:diagnosis* / Q000652:urine
keywords: Paris System for Reporting Urinary Cytology; ThinPrep method; cytospin; urinary cytology