title: Reduced RanBPM Expression Is Associated with Distant Metastasis in Gastric Cancer and Chemoresistance.
abstract: OBJECTIVE
  Ran binding protein M (RanBPM) is a ubiquitous, nucleocytoplasmic protein that serves as a scaffolding molecule. This study aimed to investigate the role of RanBPM in gastric cancer.
  
  
  METHODS
  RanBPM expression in human gastric cancer tissue samples was analyzed using real-time polymerase chain reaction. The effect of RanBPM on cellular functions was examined in RanBPM-knockdown gastric cells and with in vitro cell functional assays.
  
  
  RESULTS
  Gastric tumors with distant metastases expressed lower levels of RanBPM transcripts compared to tumours without detectable metastases (p=0.036). RanBPM knockdown in gastric cancer cells reduced adhesion and promoted survival of gastric cancer cells after exposure to methotrexate and fluorouracil.
  
  
  CONCLUSIONS
  RanBPM levels were reduced in gastric tumors with distant metastases. This suggests that loss of RanBPM expression may play an important role in gastric cancer tumor development and metastasis. Reduced RanBPM expression is also associated with chemoresistance of gastric cancer cells.
authors.affiliation: Metastasis & Angiogenesis Research Group, Cardiff-China Medical Research Collaborative (CCMRC), Division of Cancer & Genetics, Cardiff University School of Medicine, Heath Park, Cardiff, U.K.
authors.forename: Shuai
authors.initials: S
authors.lastname: Shao
authors.affiliation: Metastasis & Angiogenesis Research Group, Cardiff-China Medical Research Collaborative (CCMRC), Division of Cancer & Genetics, Cardiff University School of Medicine, Heath Park, Cardiff, U.K.
authors.forename: Ping-Hui
authors.initials: PH
authors.lastname: Sun
authors.affiliation: Metastasis & Angiogenesis Research Group, Cardiff-China Medical Research Collaborative (CCMRC), Division of Cancer & Genetics, Cardiff University School of Medicine, Heath Park, Cardiff, U.K.
authors.forename: Lucy K
authors.initials: LK
authors.lastname: Satherley
authors.affiliation: Metastasis & Angiogenesis Research Group, Cardiff-China Medical Research Collaborative (CCMRC), Division of Cancer & Genetics, Cardiff University School of Medicine, Heath Park, Cardiff, U.K. Key Laboratory of Carcinogenesis and Translational Research, Gastrointestinal Tumor Center, Peking University Cancer Hospital and Institute, Beijing, P.R. China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Gao
authors.affiliation: Metastasis & Angiogenesis Research Group, Cardiff-China Medical Research Collaborative (CCMRC), Division of Cancer & Genetics, Cardiff University School of Medicine, Heath Park, Cardiff, U.K.
authors.forename: K E
authors.initials: KE
authors.lastname: Ji
authors.affiliation: Metastasis & Angiogenesis Research Group, Cardiff-China Medical Research Collaborative (CCMRC), Division of Cancer & Genetics, Cardiff University School of Medicine, Heath Park, Cardiff, U.K.
authors.forename: Y I
authors.initials: YI
authors.lastname: Feng
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research, Gastrointestinal Tumor Center, Peking University Cancer Hospital and Institute, Beijing, P.R. China.
authors.forename: Yongning
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research, Gastrointestinal Tumor Center, Peking University Cancer Hospital and Institute, Beijing, P.R. China.
authors.forename: Jiafu
authors.initials: J
authors.lastname: Ji
authors.affiliation: Metastasis & Angiogenesis Research Group, Cardiff-China Medical Research Collaborative (CCMRC), Division of Cancer & Genetics, Cardiff University School of Medicine, Heath Park, Cardiff, U.K.
authors.forename: Wen G
authors.initials: WG
authors.lastname: Jiang
authors.affiliation: Metastasis & Angiogenesis Research Group, Cardiff-China Medical Research Collaborative (CCMRC), Division of Cancer & Genetics, Cardiff University School of Medicine, Heath Park, Cardiff, U.K. YeL@cf.ac.uk.
authors.forename: Lin
authors.initials: L
authors.lastname: Ye
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000230:Adenocarcinoma / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism* / Q000556:secondary; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D019008:Drug Resistance, Neoplasm; D005472:Fluorouracil / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D008727:Methotrexate / Q000494:pharmacology*; D009361:Neoplasm Invasiveness; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D016337:RNA, Catalytic / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors; D014162:Transfection
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000964:Antimetabolites, Antineoplastic; D003598:Cytoskeletal Proteins; D009687:Nuclear Proteins; D016337:RNA, Catalytic; C116005:Ran binding protein 9; C427204:hammerhead ribozyme; D005472:Fluorouracil; D008727:Methotrexate
keywords: RanBPM; gastric cancer; growth; invasion; matrix metalloproteinase; migration