title: Identification and differential expression of microRNAs associated with fat deposition in the liver of Wistar rats with nonalcoholic fatty liver disease.
abstract: The exact mechanism underlying hepatic steatosis in nonalcoholic fatty liver disease (NAFLD) is not clear. Clarifying the full repertoire of microRNAs (miRNAs) in NAFLD rat liver would enhance our understanding of NAFLD pathogenesis. In this study, miRNA expression levels were analyzed in liver tissue from NAFLD Wistar rats, with normal Wistar rats as negative controls. Small RNA libraries were constructed for each sample. A total of 173 conservative miRNAs and 68 potential miRNA candidates were identified. Significant differences in the expression levels of 101 conserved miRNAs were identified between the two groups. The results of GO annotation and KEGG pathway analysis revealed that some miRNAs were likely involved in the process of liver fat deposition. This study represents the first global miRNA profiling of NAFLD Wistar rat livers, and expands the miRNA repertoire for normal livers. Our findings suggest that miRNAs play important roles in liver fat deposition.
authors.affiliation: Shandong Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, Taian 271018, PR China; Laboratory Department, Taian Central Hospital, Taian 271000, PR China.
authors.forename: Deqing
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Shandong Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, Taian 271018, PR China.
authors.forename: Yuqian
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Shandong Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, Taian 271018, PR China.
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Ji
authors.affiliation: Shandong Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, Taian 271018, PR China. Electronic address: zhwang@sdau.edu.cn.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D001483:Base Sequence; D005234:Fatty Liver / Q000473:pathology*; D020869:Gene Expression Profiling; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing; D008099:Liver / Q000473:pathology*; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D065626:Non-alcoholic Fatty Liver Disease / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D017423:Sequence Analysis, RNA; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D014280:Triglycerides
keywords: Differential expression; MicroRNAs; Nonalcoholic fatty liver disease; Rat; Solexa sequencing