title: Gonadotropin ratio affects the in vitro growth of rhesus ovarian preantral follicles.
abstract: In vitro follicle growth (IVFG) strategy is critical in the fertility preservation of cancer survivors; however, its optimal protocol needs to be developed using primate models since the availability of human samples is limited. Only a few previous studies have reported the successful IVFG of rhesus monkey ovaries using low-dose follicle-stimulating hormone (FSH) (0.3 or 3 ng/mL) and long-term culture (up to 5 weeks) and it is still uncertain in regard to the optimal culture duration and effective dose of treated gonadotropins applicable to the IVFG of rhesus preantral follicles. Recently, we have reported that the FSH to luteinizing hormone (LH) ratio affects the in vitro growth of murine ovarian follicles. We aimed to investigate whether gonadotropin ratios affect the efficiency of rhesus follicular growth in vitro Ovaries were collected from six necropsied rhesus macaques (4-9 years) and preantral follicles were retrieved and cultured for 14 days using 200 mIU/mL FSH. The characteristics of follicular growth were compared between the FSH:LH=1:1 (n=24) and FSH:LH=2:1 (n=24) groups. High concentration gonadotropin treatment shortened the duration required for in vitro maturation of rhesus preantral follicles. The FSH:LH=2:1 group showed a faster follicular growth and enabled the acquisition of mature oocytes, although the expression of growth differentiation factor (GDF)-9 and anti-Müllerian hormone (AMH) did not differ significantly between the two groups. Taken together, high dose gonadotropin treatment can shorten the duration of IVFG and the gonadotropin ratio is important in the IVFG of rhesus monkey ovaries.
authors.affiliation: Department of Obstetrics and Gynecology, Seoul National University Hospital, Seoul, Republic of Korea.
authors.forename: Yoon Young
authors.initials: YY
authors.lastname: Kim
authors.affiliation: Department of Experimental Animal Research, Biomedical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea.
authors.forename: Jun-Won
authors.initials: JW
authors.lastname: Yun
authors.affiliation: Xenotransplantation Research Center, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors.forename: Jong Min
authors.initials: JM
authors.lastname: Kim
authors.affiliation: Xenotransplantation Research Center, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors.forename: Chung Gyu
authors.initials: CG
authors.lastname: Park
authors.affiliation: Center for Reproductive Medicine and Infertility, Weill Cornell Medical College, New York, New York, USA.
authors.forename: Zev
authors.initials: Z
authors.lastname: Rosenwaks
authors.affiliation: Center for Reproductive Medicine and Infertility, Weill Cornell Medical College, New York, New York, USA.
authors.forename: Hung Ching
authors.initials: HC
authors.lastname: Liu
authors.affiliation: Department of Experimental Animal Research, Biomedical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea.
authors.forename: Byeong-Cheol
authors.initials: BC
authors.lastname: Kang
authors.affiliation: Department of Obstetrics and Gynecology, Seoul National University Hospital, Seoul, Republic of Korea.
authors.forename: Seung-Yup
authors.initials: SY
authors.lastname: Ku
mesh_terms: D000818:Animals; D054304:Anti-Mullerian Hormone / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006062:Gonadotropins / Q000378:metabolism*; D055429:Growth Differentiation Factor 9 / Q000235:genetics / Q000378:metabolism; D008253:Macaca mulatta; D006080:Ovarian Follicle / Q000254:growth & development* / Q000378:metabolism*; D011962:Receptors, FSH / Q000235:genetics / Q000378:metabolism
chemical_list: D006062:Gonadotropins; D055429:Growth Differentiation Factor 9; D011962:Receptors, FSH; D054304:Anti-Mullerian Hormone
keywords: Biomedical Research