title: KLF8 knockdown triggered growth inhibition and induced cell phase arrest in human pancreatic cancer cells.
abstract: BACKGROUND
  The transcription factor Krüppel-like factor 8 (KLF8) plays an important role in tumor development and growth, but its role in pancreatic cancer (PC) is not clear.
  
  
  METHODS
  KLF8 expression in human PC cell lines and tumor tissues was measured by quantitative real-time polymerase chain reaction and Western blot analyses. The effects of lentivirus mediated knockdown of KLF8 on proliferation and growth in Panc-1 pancreatic cancer cells were examined.
  
  
  RESULTS
  KLF8 was overexpressed in 5 pancreatic cancer cell lines and in samples from patients with PC. In Panc-1 cells, KLF8 knockdown inhibited cell proliferation, tumorigenicity, and induced G2/M phase arrest. KLF8 knockdown suppressed PC tumor growth in nude mice model. Western blot analysis showed that KLF8 knockdown in Panc-1 cells down-regulated the expression of CDK1/CDC2, cyclin B1, and cyclin D1 and up-regulated the expression of p21, and p27.
  
  
  CONCLUSIONS
  Overexpression of KLF8 may contribute to the progression of pancreatic cancer, and downregulation of KLF8 expression by lentivirus-delivered shRNA is a novel therapeutic approach for PC.
authors.affiliation: Department of Radiology, Xiangya Hospital, Central South University, Hunan 410008, PR China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Yi
authors.affiliation: Department of General Surgery, Xiangya Hospital, Central South University, Hunan 410008, PR China.
authors.forename: Yixiong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of General Surgery, Xiangya Hospital, Central South University, Hunan 410008, PR China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zai
authors.affiliation: Department of Radiology, Xiangya Hospital, Central South University, Hunan 410008, PR China.
authors.forename: Xueying
authors.initials: X
authors.lastname: Long
authors.affiliation: Department of Radiology, Xiangya Hospital, Central South University, Hunan 410008, PR China. Electronic address: docterwenzhengli@126.com.
authors.forename: Wenzheng
authors.initials: W
authors.lastname: Li
mesh_terms: D000818:Animals; D016203:CDC2 Protein Kinase; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D056744:Cyclin B1 / Q000096:biosynthesis; D019938:Cyclin D1 / Q000096:biosynthesis; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000096:biosynthesis; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000096:biosynthesis; D018844:Cyclin-Dependent Kinases / Q000096:biosynthesis; D059565:G2 Phase Cell Cycle Checkpoints / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012097:Repressor Proteins / Q000235:genetics*; D014183:Transplantation, Heterologous
chemical_list: C534699:CCNB1 protein, human; C530104:CCND1 protein, human; C493956:CDKN1A protein, human; D056744:Cyclin B1; D050759:Cyclin-Dependent Kinase Inhibitor p21; C507402:KLF8 protein, human; D051741:Kruppel-Like Transcription Factors; D034741:RNA, Small Interfering; D012097:Repressor Proteins; D019938:Cyclin D1; D050760:Cyclin-Dependent Kinase Inhibitor p27; D016203:CDC2 Protein Kinase; C528899:CDK1 protein, human; D018844:Cyclin-Dependent Kinases
keywords: Cell proliferation; KLF8; Lentivirus; Pancreatic cancer; RNAi