title: Suppressive effects of primed eosinophils on single epicutaneous sensitization through regulation of dermal dendritic cells.
abstract: Eosinophils are multifunctional innate immune cells involved in many aspects of innate and adaptive immunity. Epicutaneous sensitization with protein allergen is an important sensitization route for atopic dermatitis. In this study, using a murine single protein-patch model, we show that eosinophils of a primed status accumulate in draining lymph nodes following single epicutaneous sensitization. Further, depletion of eosinophils results in enhancement of the induced Th1/Th2 immune responses, whereas IL-5-induced hypereosinophilia suppresses these responses. Mechanistically, primed eosinophils cause a reduction in the numbers and activation status of dermal dendritic cells in draining lymph nodes. Collectively, these results demonstrate that primed eosinophils exert suppressive effects on single epicutaneous sensitization through regulation of dermal dendritic cells. Thus, these findings highlight the critical roles of eosinophils in the pathogenesis of atopic dermatitis with important clinical implications for the prevention of allergen sensitization.
authors.affiliation: Department of Dermatology, Chang Gung Memorial Hospital, Keelung, Taiwan.|Chang Gung University College of Medicine, Taoyuan, Taiwan.
authors.forename: Jing-Yi
authors.initials: JY
authors.lastname: Lin
authors.affiliation: Department of Dermatology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
authors.forename: Yng-Cun
authors.initials: YC
authors.lastname: Ta
authors.affiliation: Department of Dermatology, Taipei City Hospital Heping Fuyou branch, Taipei, Taiwan.
authors.forename: I-Lin
authors.initials: IL
authors.lastname: Liu
authors.affiliation: Department of Dermatology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
authors.forename: Hsi-Wen
authors.initials: HW
authors.lastname: Chen
authors.affiliation: Department of Dermatology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Wang
mesh_terms: D000818:Animals; D003876:Dermatitis, Atopic / Q000276:immunology*; D004804:Eosinophils / Q000502:physiology*; D005260:Female; D007114:Immunization; D007801:Langerhans Cells / Q000502:physiology*; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D012867:Skin / Q000276:immunology*
keywords: Th1/Th2; dermal dendritic cells; eosinophils; epicutaneous sensitization; suppression