title: Selecting soybean resistant to the cyst nematode Heterodera glycines using simple sequence repeat (microssatellite) markers.
abstract: The soybean cyst nematode (SCN) is a major cause of soybean yield reduction. The objective of this study was to evaluate the efficiency of marker-assisted selection to identify genotypes resistant to SCN race 3 infection, using Sat_168 and Sat-141 resistance quantitative trait loci. The experiment was carried out under greenhouse conditions, using soybean populations originated from crosses between susceptible and resistant parent stock: CD-201 (susceptible) and Foster IAC (resistant), Conquista (susceptible) and S83-30 (resistant), La-Suprema (susceptible) and S57-11 (resistant), and Parecis (susceptible) and S65-50 (resistant). Plants were inoculated with SCN and evaluated according to the female index (FI), those with FI < 10% were classified as resistant to nematode infection. Plants were genotyped for SCN resistance using microsatellite markers Sat-141 and Sat_168. Marker selection efficiency was analyzed by a contingency table, taking into account genotypic versus phenotypic evaluations for each line. These markers were shown to be useful tool for selection of SCN race 3.
authors.affiliation: Instituto de Ciências Agrárias, Universidade Federal de Uberlândia, Uberlândia, MG, Brasil.
authors.forename: S M C G
authors.initials: SM
authors.lastname: Espindola
authors.affiliation: Instituto de Ciências Agrárias, Universidade Federal de Uberlândia, Uberlândia, MG, Brasil.
authors.forename: O T
authors.initials: OT
authors.lastname: Hamawaki
authors.affiliation: Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Uberlândia, MG, Brasil.
authors.forename: A P
authors.initials: AP
authors.lastname: Oliveira
authors.affiliation: Instituto Master Presidente Antonio Carlos, Araguari, MG, Brasil.
authors.forename: C D L
authors.initials: CD
authors.lastname: Hamawaki
authors.affiliation: Department of Plant, Soil & Agricultural Systems, Southern Illinois University Carbondale, IL, USA.
authors.forename: R L
authors.initials: RL
authors.lastname: Hamawaki
authors.affiliation: Instituto de Ciências Agrárias, Universidade Federal de Uberlândia, Uberlândia, MG, Brasil.
authors.forename: L M
authors.initials: LM
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D060467:Disease Resistance; D017343:Genes, Plant; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation; D007113:Immunity, Innate; D007178:Inbreeding; D018895:Microsatellite Repeats; D009348:Nematoda / Q000502:physiology*; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology; D040641:Quantitative Trait Loci; D013025:Glycine max / Q000235:genetics* / Q000276:immunology / Q000469:parasitology
chemical_list: D005819:Genetic Markers