title: Comparison of a New and Rapid Method: Brucella Coombs Gel Test With Other Diagnostic Tests.
abstract: BACKGROUND
  The aim of this study was to detect reliability of Brucella Coombs gel test (BCGT) by comparing with with ELISA (IgG + IgM), Standard agglutination test, and Brucella immunocapture agglutination methods in serological diagnosis of brucellosis.
  
  
  METHODS
  Brucella Coombs gel test (BCGT), Brucella ELISA (IgG + IgM), Standard agglutination test, and Brucella immunocapture agglutination tests of 78 patients with presumptive diagnosis of brucellosis which were sent to Microbiology Laboratory of Konya Numune Hospital from various regions of Konya were studied.
  
  
  RESULTS
  
  
  
  RESULTS
  Of 78 patients with ELISA IgG and IgM, STA, BICA and BCGT; 26, 21, 10, 12 and 12 were positive. When compared with BICA, the sensitivity and specifity of BCGT were 100% and 100%, respectively.
  
  
  CONCLUSIONS
  According to results BCGT can be used as a diagnostic test in routine laboratories after more comprehensive studies in control groups and patients.
authors.affiliation: Department of Microbiology and Clinical Microbiology, Konya Numune Hospital, Konya, Turkey. drfatmakalem@yahoo.com.
authors.forename: Fatma
authors.initials: F
authors.lastname: Kalem
authors.affiliation: Department of Microbiology and Clinical Microbiology, Konya Numune Hospital, Konya, Turkey.
authors.forename: Ayşe Gül
authors.initials: AG
authors.lastname: Ergün
authors.affiliation: Department of Microbiology and Clinical Microbiology, Konya Numune Hospital, Konya, Turkey.
authors.forename: Süleyman
authors.initials: S
authors.lastname: Durmaz
authors.affiliation: Department of Microbiology, Meram Faculty of Medicine, Necmettin Erbakan University, Konya, Turkey.
authors.forename: Metin
authors.initials: M
authors.lastname: Doğan
authors.affiliation: Department of Microbiology and Clinical Microbiology, Konya Numune Hospital, Konya, Turkey.
authors.forename: Ömür
authors.initials: Ö
authors.lastname: Ertuğrul
authors.affiliation: Department of Microbiology, Faruk Sukhan Obstetric and Gynecology Hospital, Konya, Turkey.
authors.forename: Seval
authors.initials: S
authors.lastname: Gündem
mesh_terms: D000371:Agglutination; D002002:Brucella / Q000302:isolation & purification*; D003298:Coombs Test / Q000379:methods*; D019937:Diagnostic Techniques and Procedures; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008297:Male; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M
keywords: Brucella Coombs gel test; Brucella immunocapture agglutination; Brucellosis; ELISA; Standard agglutination test