title: Microarray analysis of kiwifruit (Actinidia chinensis) bark following challenge by the sucking insect Hemiberlesia lataniae (Hemiptera: Diaspididae).
abstract: Both commercial and experimental genotypes of kiwifruit (Actinidia spp.) exhibit large differences in response to insect pests. An understanding of the vine's physiological response to insect feeding and its genetic basis will be important in assisting the development of varieties with acceptable levels of pest resistance. This experiment describes transcriptome changes observed in the bark of kiwifruit 2 and 7 days after the commencement of feeding by the armored scale insect pest, Hemiberlesia lataniae. Using a cDNA microarray consisting of 17,512 unigenes, we measured transcriptome changes and analyzed these into functional ontology categories using MapMan. Results are available in the GEO database GSE73922 and are described fully in Ref. Hill et al. (2015) [1]. After 7 days, transcripts associated with photosynthesis were down-regulated and secondary metabolism was up-regulated. Differential expression of transcripts associated with stress response was consistent with a defense response involving both effector and herbivore-triggered immunities, with predominant involvement of the salicylic acid phytohormonal pathway. This hypothesis was supported by the results of two laboratory experiments. The methods described here could be further adapted and applied to the study of plant responses to a wide range of sessile sucking pests.
authors.affiliation: New Zealand Institute for Plant & Food Research Limited (PFR), Canterbury Agriculture & Science Centre, Gerald St, Lincoln 7608, New Zealand.
authors.forename: M Garry
authors.initials: MG
authors.lastname: Hill
authors.affiliation: PFR, Private Bag 3230, Waikato Mail Centre, Hamilton 3240, New Zealand.
authors.forename: Kirstin V
authors.initials: KV
authors.lastname: Wurms
authors.affiliation: PFR, 412 No1 Rd RD2, Te Puke, New Zealand.
authors.forename: Marcus W
authors.initials: MW
authors.lastname: Davy
authors.affiliation: Zespri International Limited, 400 Maunganui Road, Mount Maunganui 3116, New Zealand.
authors.forename: Elaine
authors.initials: E
authors.lastname: Gould
authors.affiliation: PFR, Private Bag 92169, Auckland, New Zealand.
authors.forename: Andrew
authors.initials: A
authors.lastname: Allan
authors.affiliation: PFR, 412 No1 Rd RD2, Te Puke, New Zealand.
authors.forename: Nicola A
authors.initials: NA
authors.lastname: Mauchline
authors.affiliation: PFR, Private Bag 92169, Auckland, New Zealand.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Luo
authors.affiliation: PFR, Private Bag 3230, Waikato Mail Centre, Hamilton 3240, New Zealand.
authors.forename: Annette
authors.initials: A
authors.lastname: Ah Chee
authors.affiliation: PFR, 412 No1 Rd RD2, Te Puke, New Zealand.
authors.forename: Kate
authors.initials: K
authors.lastname: Stannard
authors.affiliation: PFR, 412 No1 Rd RD2, Te Puke, New Zealand.
authors.forename: Roy D
authors.initials: RD
authors.lastname: Storey
authors.affiliation: PFR, Private Bag 92169, Auckland, New Zealand.
authors.forename: Erik H
authors.initials: EH
authors.lastname: Rikkerink