title: Accumulation of a soluble form of human nectin-2 is required for exerting the resistance against herpes simplex virus type 2 infection in transfected cells.
abstract: Cell entry of herpes simplex virus type 2 (HSV-2) requires the interaction of viral glycoprotein D (gD) with the receptor nectin-1 and herpesvirus entry mediator (HVEM). In addition, it is known that nectin-2 is also functional as a receptor for HSV-2, although the binding to the gD is weak. To examine an antiviral potential of a soluble form of human nectin-2 (hNectin-2Ig), transfected Vero cells expressing the entire ectodomain of nectin-2 fused to the Fc portion of human IgG were established. Specific binding of hNectin-2Ig to HSV-2 gD was confirmed by ELISA. Competitive ELISA demonstrated that accumulation of hNectin-2Ig in transfected cells increased significantly in a cell culture time dependent manner. Viral growth of several HSV-2 strains was significantly inhibited in the transfected cells that were cultured for 72 hr compared with control Vero cells, but not in cells that were cultured for 24 hr. These results indicate that accumulation of a soluble form of nectin-2 is required for exerting the resistance against HSV-2 infection.
authors.forename: Y
authors.initials: Y
authors.lastname: Fujimoto
authors.forename: K
authors.initials: K
authors.lastname: Ozaki
authors.forename: N
authors.initials: N
authors.lastname: Iwamori
authors.forename: H
authors.initials: H
authors.lastname: Takakuwa
authors.forename: E
authors.initials: E
authors.lastname: Ono
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000276:immunology*; D002522:Chlorocebus aethiops; D006561:Herpes Simplex / Q000235:genetics / Q000276:immunology* / Q000821:virology; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D006801:Humans; D000075983:Nectins; D014162:Transfection; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D015815:Cell Adhesion Molecules; C000619010:NECTIN1 protein, human; D000075983:Nectins; D014759:Viral Envelope Proteins; C040570:glycoprotein D-herpes simplex virus type 2
keywords: antiviral effect; soluble form of nectin-2; herpes simplex virus type 2.