title: Thyroxine therapy ameliorates serum levels of eicosanoids in Chinese subclinical hypothyroidism patients.
abstract: OBJECTIVE
  The eicosanoids derived from phospholipids play key roles in inflammation. However, the profiles of serum eicosanoids in subclinical hypothyroidism (SH) patients and the effects of thyroxine replacement therapy (TRT) on these eicosanoids remain unclear. Many studies show that TSH regulates lipid metabolism. As eicosanoids derived from phospholipids play key roles in oxidative stress and immune function and inflammatory process, it was necessary to explore the profiles of serum eicosanoids in SH patients and the effects of thyroxine replacement therapy (TRT) on the eicosanoids.
  
  
  METHODS
  A total of 50 Chinese SH patients and 22 healthy volunteers were recruited. SH patients received TRT (L-T4, 25 and 50 mcg/d for patients with TSH≤10.0 mIU/L and TSH>10.0 mIU/L, respectively) for 3 months. Serum levels of major eicosanoids and cPLA2 were analyzed using LC-MS and clinical biochemical assays.
  
  
  RESULTS
  The serum levels of cPLA2, eicosanoids (8-isoPGF2a, 11-dehydroTXB2 and 12-HETE) and 11-dehydroTXB2/6-Keto-PGF1a were significantly elevated in SH patients. The serum TSH levels were significantly correlated with the levels of cPLA2 (r=+0.65), 11-dehydroTXB2 (r=+0.32) and 11-dehydroTXB2/6-Keto-PGF1a (r=+0.37). After 3-month TRT, the serum levels of TSH, cPLA2 and the above-mentioned eicosanoids in SH patients were significantly decreased.
  
  
  CONCLUSIONS
  The metabolism of eicosanoids is significantly altered in Chinese SH patients, and TRT can ameliorate the abnormalities of serum eicosanoid levels.
authors.affiliation: Division of Endocrinology and Metabolism, Shandong Provincial Hospital affiliated with Shandong University, Ji-nan 250021, China.|Baoshan Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai 201900, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Division of Endocrinology and Metabolism, Shandong Provincial Hospital affiliated with Shandong University, Ji-nan 250021, China.
authors.forename: Bing-Chang
authors.initials: BC
authors.lastname: Zhang
authors.affiliation: Division of Endocrinology and Metabolism, Shandong Provincial Hospital affiliated with Shandong University, Ji-nan 250021, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.affiliation: Division of Endocrinology and Metabolism, Shandong Provincial Hospital affiliated with Shandong University, Ji-nan 250021, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Division of Endocrinology and Metabolism, Shandong Provincial Hospital affiliated with Shandong University, Ji-nan 250021, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Division of Endocrinology and Metabolism, Shandong Provincial Hospital affiliated with Shandong University, Ji-nan 250021, China.
authors.forename: Yong-Feng
authors.initials: YF
authors.lastname: Song
authors.affiliation: Division of Endocrinology and Metabolism, Shandong Provincial Hospital affiliated with Shandong University, Ji-nan 250021, China.
authors.forename: Hai-Qing
authors.initials: HQ
authors.lastname: Zhang
authors.affiliation: Division of Endocrinology and Metabolism, Shandong Provincial Hospital affiliated with Shandong University, Ji-nan 250021, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Gao
authors.affiliation: The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health; The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University; Central Research Laboratory, The Second Hospital of Shandong University, Ji-nan 250100, China.
authors.forename: Qun-Ye
authors.initials: QY
authors.lastname: Zhang
authors.affiliation: Division of Endocrinology and Metabolism, Shandong Provincial Hospital affiliated with Shandong University, Ji-nan 250021, China.
authors.forename: Jia-Jun
authors.initials: JJ
authors.lastname: Zhao
mesh_terms: D044466:Asian People; D015777:Eicosanoids / Q000097:blood*; D005260:Female; D020249:Hormone Replacement Therapy; D006801:Humans; D007037:Hypothyroidism / Q000097:blood / Q000188:drug therapy* / Q000208:ethnology; D008297:Male; D054467:Phospholipases A2 / Q000097:blood; D013974:Thyroxine / Q000627:therapeutic use*
chemical_list: D015777:Eicosanoids; D054467:Phospholipases A2; D013974:Thyroxine