title: Neoglycoenzyme-Gated Mesoporous Silica Nanoparticles: Toward the Design of Nanodevices for Pulsatile Programmed Sequential Delivery.
abstract: We report herein the design of a stimulus-programmed pulsatile delivery system for sequential cargo release based on the use of a lactose-modified esterase as a capping agent in phenylboronic acid functionalized mesoporous silica nanoparticles. The dual-release mechanism was based on the distinct stability of the cyclic boronic acid esters formed with lactose residues and the long naturally occurring glycosylation chains in the modified neoglycoenzyme. Cargo delivery in succession was achieved using glucose and ethyl butyrate as triggers.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid , 28040 Madrid, Spain.
authors.forename: Paula
authors.initials: P
authors.lastname: Díez
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid , 28040 Madrid, Spain.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Sánchez
authors.affiliation: Instituto de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Centro Mixto Universidad Politécnica de Valencia, Universidad de Valencia , 46022 Valencia, Spain.|Departamento de Química y CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Universidad Politécnica de Valencia , Camino de Vera s/n, 46022 Valencia, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: de la Torre
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid , 28040 Madrid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Gamella
authors.affiliation: Department of Organic Chemistry I, Faculty of Chemistry, Complutense University of Madrid , 28040 Madrid, Spain.
authors.forename: Paloma
authors.initials: P
authors.lastname: Martínez-Ruíz
authors.affiliation: Instituto de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Centro Mixto Universidad Politécnica de Valencia, Universidad de Valencia , 46022 Valencia, Spain.|Departamento de Química y CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Universidad Politécnica de Valencia , Camino de Vera s/n, 46022 Valencia, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Aznar
authors.affiliation: Instituto de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Centro Mixto Universidad Politécnica de Valencia, Universidad de Valencia , 46022 Valencia, Spain.|Departamento de Química y CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Universidad Politécnica de Valencia , Camino de Vera s/n, 46022 Valencia, Spain.
authors.forename: Ramón
authors.initials: R
authors.lastname: Martínez-Máñez
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid , 28040 Madrid, Spain.|IMDEA Nanoscience, Cantoblanco Universitary City , 28049 Madrid, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Pingarrón
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid , 28040 Madrid, Spain.|IMDEA Nanoscience, Cantoblanco Universitary City , 28049 Madrid, Spain.
authors.forename: Reynaldo
authors.initials: R
authors.lastname: Villalonga
mesh_terms: D016503:Drug Delivery Systems / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D016062:Porosity; D012822:Silicon Dioxide
chemical_list: D012822:Silicon Dioxide
keywords: delivery; enzymes; esterase; glucose; mesoporous silica; nanoparticles; neoglycoenzyme