title: Generation of iPSC lines from primary human chorionic villi cells.
abstract: Primary human chorionic villi (CV) cells were used to generate the iPSC line by retroviral transduction of the four Yamanaka-factors OCT4, SOX2, KLF4 and c-MYC. Pluripotency was confirmed both in vivo and in vitro. The transcriptomes of the CV-derived iPSC lines and the human embryonic stem cell lines-H1 and H9 have a Pearson correlation of 0.929 and 0.943 respectively.
authors.affiliation: Max Planck Institute for Molecular Genetics, 14195, Berlin, Germany.
authors.forename: Björn
authors.initials: B
authors.lastname: Lichtner
authors.affiliation: Max Planck Institute for Molecular Genetics, 14195, Berlin, Germany; Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany.
authors.forename: Peggy
authors.initials: P
authors.lastname: Matz
authors.affiliation: Max Planck Institute for Molecular Genetics, 14195, Berlin, Germany; Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany. Electronic address: James.Adjaye@med.uni-duesseldorf.de.
authors.forename: James
authors.initials: J
authors.lastname: Adjaye
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation; D065150:Cellular Reprogramming; D002824:Chorionic Villi / Q000276:immunology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D000090062:Kruppel-Like Factor 4