title: Identification, cDNA Cloning, and Characterization of Luteinizing Hormone Beta Subunit (lhb) Gene in Catla catla.
abstract: Reproductive hormones play a significant role in the gonadal development and gametogenesis process of animals. In the present study luteinizing hormone beta, (lhb) subunit gene was cloned and characterized from the brain of Catla catla. The lhb full-length of cDNA sequence is 629 bp which consists of 43bp 5'-UTR (untranslated region) 447bp, ORF(open reading frame) and 139 bp of 3'-UTR respectively. The coding region of lhb gene encoded a peptide of 148 amino acids. The coding sequence of lhb gene consist of a single N-linked glycosylation site (NET) and 12 cysteine knot residues. Phylogenetic analysis of C. catla Lhβ deduced amino acid sequence showed high similarity with Carassius auratus followed by Gobiocypris rarus. 3D structure Lhβ protein comprises of five β-sheets and six coils/loops. The qPCR results revealed lhb mRNA is mainly expressed in the pituitary, ovary while moderate expression was observed in brain and testis. To best our knowledge, this is the first report on the identification, molecular characterization and structural information regarding luteinizing hormone in Indian major carp.
authors.affiliation: a Division of Fish Genetics and Biotechnology , Central Institute of Fisheries Education , Mumbai , India.
authors.forename: Mohd Ashraf
authors.initials: MA
authors.lastname: Rather
authors.affiliation: a Division of Fish Genetics and Biotechnology , Central Institute of Fisheries Education , Mumbai , India.
authors.forename: Irfan Ahmad
authors.initials: IA
authors.lastname: Bhat
authors.affiliation: a Division of Fish Genetics and Biotechnology , Central Institute of Fisheries Education , Mumbai , India.
authors.forename: Rupam
authors.initials: R
authors.lastname: Sharma
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003530:Cyprinidae / Q000235:genetics*; D004247:DNA / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D037101:Luteinizing Hormone, beta Subunit / Q000737:chemistry* / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D008958:Models, Molecular; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014018:Tissue Distribution
chemical_list: D037101:Luteinizing Hormone, beta Subunit; D004247:DNA
keywords: Catla catla; Luteinizing hormone; cloning; sequencing