title: Isolation, purification and characterization of antioxidative peptide of pearl millet (Pennisetum glaucum) protein hydrolysate.
abstract: Pearl millet (Pennisetum glaucum) is a rich source of protein, used for present study to hydrolyze protein, peptide separation and its functional activity. Antioxidative bioactive peptide was successfully identified from pearl millet using trypsin enzyme. Different antioxidative potential of isolated peptide were assessed based on activity of DPPH radical, ABTS radical, hydroxyl radical, Fe(2+) chelating ability and reducing power. Bioactive peptide separated by gel-filtration chromatography, showed the higher antioxidant activity as tested by different free radicals. The activity of pearl millet protein hydrolysate fraction was found for DPPH assay (67.66%), ABTS assay (78.81%), Fe(2+) chelating ability (51.20%), hydroxyl assay (60.95%) and reducing power (0.375nm) was further purified using reversed-phase UFLC and subjected to matrix assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF-MS) for sequential identification of the peptide. The sequence SDRDLLGPNNQYLPK was identified as antioxidant peptide.
authors.affiliation: CSIR-Institute of Himalayan Bioresource, Palampur, Himachal Pradesh, India.
authors.forename: Himani
authors.initials: H
authors.lastname: Agrawal
authors.affiliation: CSIR-Institute of Himalayan Bioresource, Palampur, Himachal Pradesh, India. Electronic address: mgupta@ihbt.res.in.
authors.forename: Robin
authors.initials: R
authors.lastname: Joshi
authors.affiliation: CSIR-Institute of Himalayan Bioresource, Palampur, Himachal Pradesh, India. Electronic address: robinjoshi@ihbt.res.in.
authors.forename: Mahesh
authors.initials: M
authors.lastname: Gupta
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D052160:Benzothiazoles / Q000378:metabolism; D002152:Calorimetry, Differential Scanning; D008970:Molecular Weight; D031782:Pennisetum / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification; D011492:Protein Hydrolysates / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013451:Sulfonic Acids / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D014357:Trypsin / Q000378:metabolism
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D010455:Peptides; D011492:Protein Hydrolysates; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D014357:Trypsin
keywords: Antioxidant; MALDI-TOF-MS; Pearl millet; Peptide purification; RP-UFLC