title: The effective combination therapy against human osteosarcoma: doxorubicin plus curcumin co-encapsulated lipid-coated polymeric nanoparticulate drug delivery system.
abstract: OBJECTIVE
  To overcome both the dose-limiting side effects of conventional chemotherapeutic agents and the therapeutic failure incurred from multidrug resistant (MDR) in osteosarcoma (OS), biodegradable lipid-coated polymeric nanoparticles (LPNs) were explored for the loading of doxorubicin (DOX) and curcumin (CUR).
  
  
  METHODS
  DOX plus CUR co-encapsulated LPNs (DOX + CUR LPNs) of mixed lipid monolayer shell and biodegradable polymer core were prepared. The cytotoxicity effect of DOX + CUR LPNs, single drug loaded LPNs, and free drug solutions were evaluated on human OS cell line KHOS cells and mice KHOS cells xenograft in vivo.
  
  
  RESULTS
  DOX + CUR LPNs displayed a curative effect on OS cell lines than the free drug counterparts. Also, best anti-OS effects were observed on the animal model compared with other groups tested.
  
  
  CONCLUSIONS
  This promising dual drugs co-encapsulated lipid-coated polymeric nanoparticulate drug delivery system enhanced the cell delivery and activity of drugs against human OS cancer cell lines and in cancer bearing mice. This research may offer new options for the treatment of OS.
authors.affiliation: a Department of Orthopaedics , Shandong Provincial Hospital Affiliated to Shandong University , Ji'nan , P.R. China.|b Department of Orthopaedics , Affiliated Hospital of Taishan Medical University , Taian , P.R. China , and.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: c Department of Orthopaedics , General Hospital of Ji'nan Military Command , Ji'nan , P.R. China.
authors.forename: WeiGuo
authors.initials: W
authors.lastname: Wang
authors.affiliation: b Department of Orthopaedics , Affiliated Hospital of Taishan Medical University , Taian , P.R. China , and.
authors.forename: Ze
authors.initials: Z
authors.lastname: Rui
authors.affiliation: a Department of Orthopaedics , Shandong Provincial Hospital Affiliated to Shandong University , Ji'nan , P.R. China.
authors.forename: DongSheng
authors.initials: D
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D003474:Curcumin / Q000008:administration & dosage / Q000737:chemistry*; D004317:Doxorubicin / Q000008:administration & dosage / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D018432:Drug Resistance, Multiple / Q000187:drug effects; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D012516:Osteosarcoma / Q000188:drug therapy*; D011108:Polymers / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D008055:Lipids; D011108:Polymers; D004317:Doxorubicin; D003474:Curcumin
keywords: Co-encapsulated; combination therapy; drug delivery system; human osteosarcoma; lipid-coated polymeric nanoparticle