title: Indirect Detection of Glycosidases Using Amperometry.
abstract: Glycosidases are essential enzymes that cleave glycoside bonds. The presence of glycosidases have been widely used to detect pathogens, label cells/tissues, and report specific diseases. We have developed a rapid electrochemical assay to detect glycosidases. Exposure of electrochemically inactive substrates to glycosidases releases glucose, which can be measured easily using an electrochemical cell. Five different glycosidases were detected rapidly within 1 h using disposable electrodes. This assay could readily be incorporated into repurposed glucose meters to rapidly detect glycosidases, which in turn could be useful to report the presence of a pathogen or illness.
authors.affiliation: Department of Chemistry, Center for Diagnostics and Therapeutics, Georgia State University , 788 Petit Science Center, Atlanta, Georgia 30302, United States.
authors.forename: Bharat P
authors.initials: BP
authors.lastname: Gurale
authors.affiliation: Department of Chemistry, Center for Diagnostics and Therapeutics, Georgia State University , 788 Petit Science Center, Atlanta, Georgia 30302, United States.
authors.forename: Abasaheb N
authors.initials: AN
authors.lastname: Dhawane
authors.affiliation: Department of Chemistry, Center for Diagnostics and Therapeutics, Georgia State University , 788 Petit Science Center, Atlanta, Georgia 30302, United States.
authors.forename: Xikai
authors.initials: X
authors.lastname: Cui
authors.affiliation: Department of Chemistry, Center for Diagnostics and Therapeutics, Georgia State University , 788 Petit Science Center, Atlanta, Georgia 30302, United States.
authors.forename: Amrita
authors.initials: A
authors.lastname: Das
authors.affiliation: Department of Chemistry, Center for Diagnostics and Therapeutics, Georgia State University , 788 Petit Science Center, Atlanta, Georgia 30302, United States.
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Center for Diagnostics and Therapeutics, Georgia State University , 788 Petit Science Center, Atlanta, Georgia 30302, United States.
authors.forename: Suri S
authors.initials: SS
authors.lastname: Iyer
mesh_terms: D004209:Disposable Equipment; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D005947:Glucose / Q000032:analysis / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism / Q000652:urine*; D006801:Humans; D013997:Time Factors
chemical_list: D006026:Glycoside Hydrolases; D005947:Glucose