title: Determination of six polyether antibiotic residues in foods of animal origin by solid phase extraction combined with liquid chromatography-tandem mass spectrometry.
abstract: A new method using solid phase extraction (SPE) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed for the determination of six polyether antibiotics, including lasalocid, salinomycin, monensin, narasin, madubamycin and nigericin residues, in foods of animal origin. The samples were extracted with acetonitrile and purified by ENVI-Carb SPE columns after comparing the impurity effect and maneuverability of several SPE cartridges. Subsequently, the analytes were separated on a Hypersil Gold column (2.1×150mm, 5μm) and analyzed by MS/MS detection. The limit of quantization (LOQ) for milk and chicken was 0.4μg/kg, and for chicken livers and eggs, it was 1μg/kg. The linearity was satisfactory with a correlation coefficient of >0.9995 at concentrations ranging from 2 to 100μg/L. The average recoveries of the analytes fortified at three levels ranged from 68.2 to 114.3%, and the relative standard deviations ranged from 4.5 to 12.1%. The method was suitable for quantitative analysis and confirmation of polyether antibiotic residues in foods of animal origin.
authors.affiliation: College of Chemical & Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang 050018 Hebei Province, China; Hebei Research Center of Pharmaceutical and Chemical Engineering, Hebei University of Science and Technology, China; State Key Laboratory Breeding Base-Hebei Province Key Laboratory of Molecular Chemistry for Drug, Hebei University of Science and Technology, Shijiazhuang 050018, China. Electronic address: hajing02@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Ha
authors.affiliation: College of Chemical & Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang 050018 Hebei Province, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Song
authors.affiliation: Hebei Entry-exit Inspection and Quarantion Bureau, Shijiazhuang 050000, China.
authors.forename: Lian-Feng
authors.initials: LF
authors.lastname: Ai
authors.affiliation: College of Chemical & Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang 050018 Hebei Province, China.
authors.forename: Jian-Chen
authors.initials: JC
authors.lastname: Li
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D005502:Food; D057230:Limit of Detection; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents
keywords: Foods of animal origin; LC–MS/MS; Polyether antibiotics; SPE