title: A Transgenic Mouse Model of Poliomyelitis.
abstract: Transgenic mice (tg mice) that express the human poliovirus receptor (PVR), CD155, are susceptible to poliovirus and develop a neurological disease that resembles human poliomyelitis. Assessment of the neurovirulence levels of poliovirus strains, including mutant viruses produced by reverse genetics, circulating vaccine-derived poliovirus, and vaccine candidates, is useful for basic research of poliovirus pathogenicity, the surveillance of circulating polioviruses, and the quality control of oral live poliovirus vaccines, and does not require the use of monkeys. Furthermore, PVR-tg mice are useful for studying poliovirus tissue tropism and host immune responses. PVR-tg mice can be bred with mice deficient in the genes involved in viral pathogenicity. This report describes the methods used to analyze the pathogenicity and immune responses of poliovirus using the PVR-tg mouse model.
authors.affiliation: Neurovirology Project, Tokyo Metropolitan Institute of Medical Science, 2-1-6, Kamikitazawa, Setagaya-ku, Tokyo, 156-8506, Japan. koike-st@igakuken.or.jp.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Koike
authors.affiliation: National Institute of Infectious Diseases, 4-7-1, Gakuen, Musashimurayama, Tokyo, 208-0011, Japan.
authors.forename: Noriyo
authors.initials: N
authors.lastname: Nagata
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology; D004195:Disease Models, Animal; D060005:Genotyping Techniques / Q000379:methods; D006801:Humans; D007113:Immunity, Innate; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009154:Mutation; D011051:Poliomyelitis / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology* / Q000473:pathology; D017955:Poliovirus / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D011991:Receptors, Virus / Q000235:genetics
chemical_list: D000956:Antigens, Viral; D011991:Receptors, Virus; C066100:poliovirus receptor
keywords: Innate immune response; Intracerebral inoculation; Knockout mouse; Motor neuron; Neurovirulence; Paralysis; Poliovirus receptor; Rodent model; Tissue tropism; Viral antigen