title: Occurrence of Mesocestoides canislagopodis (Rudolphi, 1810) (Krabbe, 1865) in mammals and birds in Iceland and its molecular discrimination within the Mesocestoides species complex.
abstract: The life cycle of Mesocestoides tapeworms (Cestoda: Cyclophyllidea: Mesocestoididae) requires three hosts. The first intermediate host is unknown but believed to be an arthropod. The second intermediate host is a vertebrate. The primary definitive host is a carnivore mammal, or a bird of prey, that eats the tetrathyridium-infected second intermediate host. One representative of the genus, Mesocestoides canislagopodis, has been reported from Iceland. It is common in the arctic fox (Vulpes lagopus) and has also been detected in domestic dogs (Canis familiaris) and cats (Felis domestica). Recently, scolices of a non-maturing Mesocestoides sp. have also been detected in gyrfalcon (Falco rusticolus) intestines, and tetrathyridia in the body cavity of rock ptarmigan (Lagopus muta). We examined the taxonomic relationship of Mesocestoides from arctic fox, gyrfalcon, and rock ptarmigan using molecular methods, both at the generic level (D1 domain LSU ribosomal DNA) and at the specific level (cytochrome c oxidase subunit I (COI) and 12S mitochondrial DNA). All stages belonged to Mesocestoides canislagopodis. Phylogenetic analysis of the combined 12S-COI at the specific level confirmed that M. canislagopodis forms a distinct clade, well separated from three other recognized representatives of the genus, M. litteratus, M. lineatus, and M. corti/vogae. This is the first molecular description of this species. The rock ptarmigan is a new second intermediate host record, and the gyrfalcon a new primary definitive host record. However, the adult stage seemed not to be able to mature in the gyrfalcon, and successful development is probably restricted to mammalian hosts.
authors.affiliation: Laboratory of Parasitology, Institute for Experimental Pathology, University of Iceland, Keldur, IS-112, Reykjavík, Iceland. karlsk@hi.is.
authors.forename: Karl
authors.initials: K
authors.lastname: Skirnisson
authors.affiliation: EA4688 « Vecpar », UFR de Pharmacie, Université de Reims Champagne-Ardenne, 51 rue Cognacq-Jay, 51096, Reims Cedex, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Jouet
authors.affiliation: EA4688 « Vecpar », UFR de Pharmacie, Université de Reims Champagne-Ardenne, 51 rue Cognacq-Jay, 51096, Reims Cedex, France.
authors.forename: Hubert
authors.initials: H
authors.lastname: Ferté
authors.affiliation: Icelandic Institute of Natural History, Urriðaholtsstræti 6-8, 210, Garðabær, Iceland.
authors.forename: Ólafur K
authors.initials: ÓK
authors.lastname: Nielsen
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000469:parasitology; D002415:Cats; D002590:Cestode Infections / Q000453:epidemiology / Q000662:veterinary*; D004272:DNA, Mitochondrial / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004283:Dog Diseases / Q000469:parasitology; D004285:Dogs; D046093:Falconiformes / Q000469:parasitology*; D005589:Foxes / Q000469:parasitology*; D046094:Galliformes / Q000469:parasitology*; D007055:Iceland / Q000453:epidemiology; D008645:Mesocestoides / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification*; D017720:Molecular Epidemiology; D010802:Phylogeny
chemical_list: D004272:DNA, Mitochondrial; D016054:DNA, Protozoan; D004275:DNA, Ribosomal
keywords: Falco rusticolus; Iceland; Lagopus muta; Mesocestoides canislagopodis; Molecular biology; Tetrathyridia; Vulpes lagopus