title: Pressurized liquid extraction of Aglaonema sp. iminosugars: Chemical composition, bioactivity, cell viability and thermal stability.
abstract: Pressurized liquid extraction of Aglaonema sp. iminosugars has been optimized. A single cycle under optimal conditions (80mg, 100°C, 2min) was enough to extract ⩾96% of most iminosugars. Further incubation with Saccharomyces cerevisiae for 5h removed coextracted interfering low molecular weight carbohydrates from extracts of different Aglaonema cultivars. A complete characterization of these extracts was carried out by gas chromatography-mass spectrometry: three iminosugars were tentatively identified for the first time; α-homonojirimycin and 2,5-dideoxy-2,5-imino-d-mannitol were the major iminosugars determined. α-Glucosidase inhibition activity, cell viability and thermal stability of Aglaonema extracts were also evaluated. Extracts with IC50 for α-glucosidase activity in the 0.010-0.079mgmL(-1) range showed no decrease of Caco-2 cell viability at concentrations lower than 125μgmL(-1) and were stable at 50°C for 30days. These results highlight the potential of Aglaonema extracts as a source of bioactives to be used as functional ingredients.
authors.affiliation: Instituto de Química Orgánica General (CSIC), Juan de la Cierva, 3, 28006 Madrid, Spain. Electronic address: sonia.rodriguez@iqog.csic.es.
authors.forename: S
authors.initials: S
authors.lastname: Rodríguez-Sánchez
authors.affiliation: Instituto de Química Orgánica General (CSIC), Juan de la Cierva, 3, 28006 Madrid, Spain. Electronic address: amartinortiz@iqog.csic.es.
authors.forename: A
authors.initials: A
authors.lastname: Martín-Ortiz
authors.affiliation: Instituto de Química Orgánica General (CSIC), Juan de la Cierva, 3, 28006 Madrid, Spain. Electronic address: c.carrero@iqog.csic.es.
authors.forename: C
authors.initials: C
authors.lastname: Carrero-Carralero
authors.affiliation: Instituto de Ciencia y Tecnología de Alimentos y Nutrición (CSIC), José Antonio Novais, 10, 28040 Madrid, Spain. Electronic address: s.ramos@ictan.csic.es.
authors.forename: S
authors.initials: S
authors.lastname: Ramos
authors.affiliation: Instituto de Química Orgánica General (CSIC), Juan de la Cierva, 3, 28006 Madrid, Spain. Electronic address: mlsanz@iqog.csic.es.
authors.forename: M L
authors.initials: ML
authors.lastname: Sanz
authors.affiliation: Instituto de Química Orgánica General (CSIC), Juan de la Cierva, 3, 28006 Madrid, Spain. Electronic address: acsoria@iqog.csic.es.
authors.forename: A C
authors.initials: AC
authors.lastname: Soria
mesh_terms: D017485:1-Deoxynojirimycin / Q000031:analogs & derivatives / Q000032:analysis; D029064:Araceae / Q000737:chemistry*; D018938:Caco-2 Cells; D002241:Carbohydrates / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects*; D055598:Chemical Phenomena; D008401:Gas Chromatography-Mass Spectrometry; D065089:Glycoside Hydrolase Inhibitors / Q000032:analysis* / Q000494:pharmacology; D006801:Humans; D050112:Imino Pyranoses / Q000032:analysis; D008353:Mannitol / Q000031:analogs & derivatives / Q000032:analysis; D008970:Molecular Weight; D010936:Plant Extracts / Q000032:analysis* / Q000494:pharmacology; D011312:Pressure; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: C494911:2,5-dideoxy-2,5-imino-D-mannitol; D002241:Carbohydrates; D065089:Glycoside Hydrolase Inhibitors; D050112:Imino Pyranoses; D010936:Plant Extracts; C106502:homonojirimycin; D017485:1-Deoxynojirimycin; D008353:Mannitol; D000520:alpha-Glucosidases
keywords: Aglaonema sp.; Cell viability; Gas chromatography–mass spectrometry; Iminosugars; Pressurized liquid extraction (PLE); Thermal stability; α-Glucosidase inhibition