title: A Protein Complex Map of Trypanosoma brucei.
abstract: The functions of the majority of trypanosomatid-specific proteins are unknown, hindering our understanding of the biology and pathogenesis of Trypanosomatida. While protein-protein interactions are highly informative about protein function, a global map of protein interactions and complexes is still lacking for these important human parasites. Here, benefiting from in-depth biochemical fractionation, we systematically interrogated the co-complex interactions of more than 3354 protein groups in procyclic life stage of Trypanosoma brucei, the protozoan parasite responsible for human African trypanosomiasis. Using a rigorous methodology, our analysis led to identification of 128 high-confidence complexes encompassing 716 protein groups, including 635 protein groups that lacked experimental annotation. These complexes correlate well with known pathways as well as for proteins co-expressed across the T. brucei life cycle, and provide potential functions for a large number of previously uncharacterized proteins. We validated the functions of several novel proteins associated with the RNA-editing machinery, identifying a candidate potentially involved in the mitochondrial post-transcriptional regulation of T. brucei. Our data provide an unprecedented view of the protein complex map of T. brucei, and serve as a reliable resource for further characterization of trypanosomatid proteins. The presented results in this study are available at: www.TrypsNetDB.org.
authors.affiliation: Institute of Parasitology, McGill University, Ste. Anne de Bellevue, Quebec, Canada.
authors.forename: Vahid H
authors.initials: VH
authors.lastname: Gazestani
authors.affiliation: Institute of Parasitology, McGill University, Ste. Anne de Bellevue, Quebec, Canada.
authors.forename: Najmeh
authors.initials: N
authors.lastname: Nikpour
authors.affiliation: Institute of Parasitology, McGill University, Ste. Anne de Bellevue, Quebec, Canada.|Department of Biochemistry, McGill University, Montreal, Quebec, Canada.
authors.forename: Vaibhav
authors.initials: V
authors.lastname: Mehta
authors.affiliation: Institute of Parasitology, McGill University, Ste. Anne de Bellevue, Quebec, Canada.|McGill Centre for Bioinformatics, McGill University, Montreal, Quebec, Canada.
authors.forename: Hamed S
authors.initials: HS
authors.lastname: Najafabadi
authors.affiliation: Institute of Parasitology, McGill University, Ste. Anne de Bellevue, Quebec, Canada.|Department of Biochemistry, McGill University, Montreal, Quebec, Canada.
authors.forename: Houtan
authors.initials: H
authors.lastname: Moshiri
authors.affiliation: Institute of Parasitology, McGill University, Ste. Anne de Bellevue, Quebec, Canada.|Centre for Host-Parasite Interactions, Institute of Parasitology, McGill University, Ste. Anne de Bellevue, Quebec, Canada.
authors.forename: Armando
authors.initials: A
authors.lastname: Jardim
authors.affiliation: Institute of Parasitology, McGill University, Ste. Anne de Bellevue, Quebec, Canada.|Department of Biochemistry, McGill University, Montreal, Quebec, Canada.|McGill Centre for Bioinformatics, McGill University, Montreal, Quebec, Canada.
authors.forename: Reza
authors.initials: R
authors.lastname: Salavati
mesh_terms: D002852:Chromatography, Ion Exchange; D005786:Gene Expression Regulation / Q000502:physiology*; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D015203:Reproducibility of Results; D059467:Transcriptome; D014346:Trypanosoma brucei brucei / Q000235:genetics / Q000378:metabolism*
chemical_list: D015800:Protozoan Proteins