title: Expression analysis of ClpB/Hsp100 gene in faba bean (Vicia faba L.) plants in response to heat stress.
abstract: Heat stress adversely affects the growth and yield of faba bean crop. Accumulation of ClpB/Hsp100 class of proteins is a critical parameter in induction of acquired heat stress tolerance in plants. Heat-induced expression of ClpB/Hsp100 genes has been noted in diverse plant species. Using primers complementary to soybean ClpB/Hsp100 gene, we analyzed the transcript expression profile of faba bean ClpB/Hsp100 gene in leaves of seedlings and flowering plants and in pollen grains. ClpB/Hsp100 protein accumulation profile was analyzed in leaves of faba bean seedlings using Arabidopsis thaliana cytoplasmic Hsp101 antibodies. The transcript and protein levels of faba bean ClpB/Hsp100 were significantly induced in response to heat stress.
authors.affiliation: Department of Plant Molecular Biology, University of Delhi, South Campus, New Delhi 110021, India.
authors.forename: Ritesh
authors.initials: R
authors.lastname: Kumar
authors.affiliation: Department of Plant Molecular Biology, University of Delhi, South Campus, New Delhi 110021, India.
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Singh
authors.affiliation: Department of Plant Molecular Biology, University of Delhi, South Campus, New Delhi 110021, India.
authors.forename: Dhruv
authors.initials: D
authors.lastname: Lavania
authors.affiliation: Department of Botany and Microbiology, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Manzer H
authors.initials: MH
authors.lastname: Siddiqui
authors.affiliation: Department of Botany and Microbiology, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Mohamed H
authors.initials: MH
authors.lastname: Al-Whaibi
authors.affiliation: Department of Plant Molecular Biology, University of Delhi, South Campus, New Delhi 110021, India.
authors.forename: Anil
authors.initials: A
authors.lastname: Grover
keywords: ClpB; Faba bean; Heat stress; Hsp100; Pollen; Protein expression; Thermotolerance; Transcript induction