title: Lactic Acid Bacteria Improves Peyer's Patch Cell-Mediated Immunoglobulin A and Tight-Junction Expression in a Destructed Gut Microbial Environment.
abstract: To evaluate the effects of lactic acid bacteria (LAB) on Peyer's patch cells, mice were treated with a high dose of kanamycin to disturb the gut microbial environment. The overarching goal was to explore the potential of LAB for use as a dietary probiotic that buffers the negative consequences of antibiotic treatment. In vitro, LAB stimulated the production of immunoglobulin A (IgA) from isolated Peyer's patch cells. Inflammation-related genes (TNF-α, IL-1β, and IL-8) were up-regulated in Caco-2 cells stimulated with lipopolysaccharide (LPS), while tight-junction-related genes (ZO-1 and occludin) were down-regulated; the effects of LPS on inflammatory gene and tight-junction gene expression were reversed by treatment with LAB. Mice treated with a high dose of kanamycin showed increased serum IgE levels and decreases in serum IgA and fecal IgA levels; the number of Peyer's patch cells decreased with kanamycin treatment. However, subsequent LAB treatment was effective in reducing the serum IgE level and recovering the serum IgA and fecal IgA levels, as well as the number of Peyer's patch cells. In addition, ZO-1 and occludin mRNA levels were up-regulated in the ileum tissues of mice receiving LAB treatment. Lactic acid bacteria can enhance the intestinal immune system by improving the integrity of the intestinal barrier and increasing the production of IgA in Peyer's patches. Lactic acid bacteria should be considered a potential probiotic candidate for improving intestinal immunity, particularly in mitigating the negative consequences of antibiotic use.
authors.affiliation: R&BD Center, Korea Yakult Co. Ltd., Yongin 17086, Republic of Korea.
authors.forename: Sung Hwan
authors.initials: SH
authors.lastname: Kim
authors.affiliation: R&BD Center, Korea Yakult Co. Ltd., Yongin 17086, Republic of Korea.
authors.forename: Woonhee
authors.initials: W
authors.lastname: Jeung
authors.affiliation: R&BD Center, Korea Yakult Co. Ltd., Yongin 17086, Republic of Korea.
authors.forename: Il-Dong
authors.initials: ID
authors.lastname: Choi
authors.affiliation: R&BD Center, Korea Yakult Co. Ltd., Yongin 17086, Republic of Korea.
authors.forename: Ji-Woong
authors.initials: JW
authors.lastname: Jeong
authors.affiliation: R&BD Center, Korea Yakult Co. Ltd., Yongin 17086, Republic of Korea.
authors.forename: Dong Eun
authors.initials: DE
authors.lastname: Lee
authors.affiliation: College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.|Research Institute of Eco-friendly Animal Sciences, Institute of Green Bio Science & Technology, Seoul National University, Pyeongchang 25354, Republic of Korea.
authors.forename: Chul-Sung
authors.initials: CS
authors.lastname: Huh
authors.affiliation: Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Republic of Korea.
authors.forename: Geun-Bae
authors.initials: GB
authors.lastname: Kim
authors.affiliation: Division of Gastroenterology, Vievisnamuh Hospital, Seoul 06117, Republic of Korea.
authors.forename: Seong Soo
authors.initials: SS
authors.lastname: Hong
authors.affiliation: R&BD Center, Korea Yakult Co. Ltd., Yongin 17086, Republic of Korea.|College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Jae-Jung
authors.initials: JJ
authors.lastname: Shim
authors.affiliation: R&BD Center, Korea Yakult Co. Ltd., Yongin 17086, Republic of Korea.|College of Veternary Medicine and Veternary Science Research Institute, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Jung Lyoul
authors.initials: JL
authors.lastname: Lee
authors.affiliation: R&BD Center, Korea Yakult Co. Ltd., Yongin 17086, Republic of Korea.
authors.forename: Jae-Hun
authors.initials: JH
authors.lastname: Sim
authors.affiliation: R&BD Center, Korea Yakult Co. Ltd., Yongin 17086, Republic of Korea.
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Ahn
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000009:adverse effects; D018938:Caco-2 Cells; D000069196:Gastrointestinal Microbiome; D006801:Humans; D007070:Immunoglobulin A / Q000096:biosynthesis / Q000097:blood / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood; D053583:Interleukin-1beta / Q000235:genetics; D016209:Interleukin-8 / Q000235:genetics; D007612:Kanamycin / Q000008:administration & dosage / Q000009:adverse effects; D007778:Lactobacillus / Q000502:physiology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D062793:Occludin / Q000235:genetics; D010581:Peyer's Patches / Q000166:cytology / Q000276:immunology* / Q000382:microbiology / Q000502:physiology*; D019936:Probiotics / Q000627:therapeutic use*; D019108:Tight Junctions / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D062826:Zonula Occludens-1 Protein / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D007070:Immunoglobulin A; D053583:Interleukin-1beta; D016209:Interleukin-8; D008070:Lipopolysaccharides; D062793:Occludin; C569043:Tjp1 protein, mouse; D014409:Tumor Necrosis Factor-alpha; D062826:Zonula Occludens-1 Protein; D007073:Immunoglobulin E; D007612:Kanamycin
keywords: Caco-2 cells; IgA; Lactic acid bacteria; Peyer's patch; kanamycin; tight junction