title: Sialylation of vitronectin regulates stress fiber formation and cell spreading of dermal fibroblasts via a heparin-binding site.
abstract: Vitronectin (VN) plays an important role in tissue regeneration. We previously reported that VN from partial hepatectomized (PH) rats results in a decrease of sialylation of VN and de-sialylation of VN decreases the cell spreading of hepatic stellate cells. In this study, we analyzed the mechanism how sialylation of VN regulates the properties of mouse primary cultured dermal fibroblasts (MDF) and a dermal fibroblast cell line, Swiss 3T3 cells. At first, we confirmed that VN from PH rats or de-sialylated VN also decreased cell spreading in MDF and Swiss 3T3 cells. The de-sialylation suppressed stress fiber formation in Swiss 3T3 cells. Next, we analyzed the effect of the de-sialylation of VN on stress fiber formation in Swiss 3T3 cells. RGD peptide, an inhibitor for a cell binding site of VN, did not affect the cell attachment of Swiss 3T3 cells on untreated VN but significantly decreased it on de-sialylated VN, suggesting that the de-sialylation attenuates the binding activity of an RGD-independent binding site in VN. To analyze a candidate RGD-independent binding site, an inhibition experiment of stress fiber formation for a heparin binding site was performed. The addition of heparin and treatment of cells with heparinase decreased stress fiber formation in Swiss 3T3 cells. Furthermore, de-sialylation increased the binding activity of VN to heparin, as detected by surface plasmon resonance (SPR). These results demonstrate that sialylation of VN glycans regulates stress fiber formation and cell spreading of dermal fibroblast cells via a heparin binding site.
authors.affiliation: The Graduate School of Humanities and Sciences and The Glycoscience Institute, Ochanomizu University, Tokyo, 112-8610, Japan. miyamoto.yasunori@ocha.ac.jp.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Miyamoto
authors.affiliation: The Graduate School of Humanities and Sciences and The Glycoscience Institute, Ochanomizu University, Tokyo, 112-8610, Japan.
authors.forename: Mio
authors.initials: M
authors.lastname: Tanabe
authors.affiliation: The Graduate School of Humanities and Sciences and The Glycoscience Institute, Ochanomizu University, Tokyo, 112-8610, Japan.
authors.forename: Kimie
authors.initials: K
authors.lastname: Date
authors.affiliation: The Graduate School of Humanities and Sciences and The Glycoscience Institute, Ochanomizu University, Tokyo, 112-8610, Japan.
authors.forename: Kanoko
authors.initials: K
authors.lastname: Sakuda
authors.affiliation: The Graduate School of Humanities and Sciences and The Glycoscience Institute, Ochanomizu University, Tokyo, 112-8610, Japan.
authors.forename: Kotone
authors.initials: K
authors.lastname: Sano
authors.affiliation: The Graduate School of Humanities and Sciences and The Glycoscience Institute, Ochanomizu University, Tokyo, 112-8610, Japan.
authors.forename: Haruko
authors.initials: H
authors.lastname: Ogawa
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001665:Binding Sites; D020405:Dermis / Q000166:cytology / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006031:Glycosylation; D051379:Mice; D051381:Rats; D022502:Stress Fibers / Q000378:metabolism*; D013552:Swine; D019096:Vitronectin / Q000378:metabolism*
chemical_list: D019096:Vitronectin
keywords: Cell adhesion; Fibroblast; Glycosylation; Heparan sulfate; Surface plasmon resonance (SPR)