title: RNA Study Using DNA Nanotechnology.
abstract: Transcription is one of the fundamental steps of gene expression, where RNA polymerases (RNAPs) bind to their template genes and make RNAs. In addition to RNAP and the template gene, many molecules such as transcription factors are involved. The interaction and the effect of these factors depend on the geometry. Molecular layout of these factors, RNAP and gene is thus important. DNA nanotechnology is a promising technology that allows controlling of the molecular layout in the range of nanometer to micrometer scale with nanometer resolution; thus, it is expected to expand the RNA study beyond the current limit.
authors.affiliation: Institute for Integrated Cell-Material Sciences, Kyoto University, Kyoto, Japan; Graduate School of Frontier Science, The University of Tokyo, Chiba, Japan. Electronic address: tadakuma.hisashi.8s@kyoto-u.ac.jp.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Tadakuma
authors.affiliation: Graduate School of Frontier Science, The University of Tokyo, Chiba, Japan.
authors.forename: Takeya
authors.initials: T
authors.lastname: Masubuchi
authors.affiliation: Graduate School of Frontier Science, The University of Tokyo, Chiba, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Ueda
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D012313:RNA / Q000737:chemistry / Q000378:metabolism*
chemical_list: D012313:RNA; D004247:DNA
keywords: DNA nanostructure; DNA origami; RNA polymerase; atomic force microscopy (AFM); single-molecule imaging