title: Age-related changes in the transcriptome of antibody-secreting cells.
abstract: We analyzed age-related defects in B cell populations from young and aged mice. Microarray analysis of bone marrow resident antibody secreting cells (ASCs) showed significant changes upon aging, affecting multiple genes, pathways and functions including those that play a role in immune regulation, humoral immune responses, chromatin structure and assembly, cell metabolism and the endoplasmic reticulum (ER) stress response. Further analysis showed upon aging defects in energy production through glucose catabolism with reduced oxidative phosphorylation. In addition aged B cells had increased levels of reactive oxygen-species (ROS), which was linked to enhanced expression of the co-inhibitor programmed cell death (PD)-1.
authors.affiliation: Gene Therapy and Vaccines Program, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA.|The Wistar Institute Vaccine Center, Philadelphia, Pennsylvania, USA.
authors.forename: Senthil
authors.initials: S
authors.lastname: Kannan
authors.affiliation: The Wistar Institute Vaccine Center, Philadelphia, Pennsylvania, USA.|Department of Biomedical and Health Informatics, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.
authors.forename: Noor
authors.initials: N
authors.lastname: Dawany
authors.affiliation: Gene Therapy and Vaccines Program, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA.
authors.forename: Raj
authors.initials: R
authors.lastname: Kurupati
authors.affiliation: The Wistar Institute Vaccine Center, Philadelphia, Pennsylvania, USA.
authors.forename: Louise C
authors.initials: LC
authors.lastname: Showe
authors.affiliation: The Wistar Institute Vaccine Center, Philadelphia, Pennsylvania, USA.
authors.forename: Hildegund C J
authors.initials: HC
authors.lastname: Ertl
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D000921:Antibody-Producing Cells / Q000166:cytology / Q000378:metabolism*; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D059865:Endoplasmic Reticulum Stress / Q000235:genetics; D051379:Mice; D010085:Oxidative Phosphorylation; D018384:Oxidative Stress / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction; D059467:Transcriptome
chemical_list: D017382:Reactive Oxygen Species
keywords: ASCs; B cells; Gerotarget; cell metabolism; oxidative phosphorylation; programmed cell death