title: Frequency of Fluoride Dentifrice Use and Caries Lesions Inhibition and Repair.
abstract: The clinical relevance of the frequency of fluoride dentifrice (FD) use on enamel caries is based on evidence. However, the relative effect of FD on reduction of demineralization or enhancement of remineralization is unknown and the effect of frequency on root dentine caries has not been explored. The aim of this double-blind, crossover, in situ study, which was conducted in 4 phases of 14 days each, was to evaluate the relationship between the frequency of FD use and enamel and root dentine de- and remineralization. Eighteen volunteers wore palatal appliances containing enamel and root dentine slabs, either sound or carious. Biofilm accumulation on the slab surface was allowed, and 20% sucrose solution was dripped 3 or 8 times per day on the carious and sound slabs, respectively. Volunteers used FD (1,100 μg F/g) in the frequencies 0 (fluoride-placebo dentifrice), 1, 2 and 3 times per day. The demineralization and remineralization that occurred in sound or carious slabs was estimated by the percentage of surface hardness loss (%SHL) or recovery (%SHR). Loosely (CaF2) and firmly (FAp) bound fluoride concentrations were also determined. The relationship between the variables was analyzed by linear regression. The %SHL, CaF2 and FAp concentrations were a function of the frequency of FD use for enamel and dentine, but the %SHR was a function of the frequency of FD use only for enamel (p < 0.05). The results suggest that demineralization in enamel and root dentine is reduced in proportion to the frequency of FD use, but for remineralization the effect of the frequency of FD use was relevant only to enamel.
authors.affiliation: Piracicaba Dental School, UNICAMP, Piracicaba, Brazil.
authors.forename: Diego Figueiredo
authors.initials: DF
authors.lastname: Nóbrega
authors.forename: Constanza Estefany
authors.initials: CE
authors.lastname: Fernández
authors.forename: Altair Antoninha
authors.initials: AA
authors.lastname: Del Bel Cury
authors.forename: Livia Maria Andaló
authors.initials: LM
authors.lastname: Tenuta
authors.forename: Jaime Aparecido
authors.initials: JA
authors.lastname: Cury
mesh_terms: D000293:Adolescent; D000328:Adult; D018441:Biofilms / Q000187:drug effects; D002327:Cariostatic Agents / Q000008:administration & dosage* / Q000494:pharmacology; D018592:Cross-Over Studies; D003731:Dental Caries / Q000188:drug therapy* / Q000473:pathology; D003743:Dental Enamel / Q000187:drug effects / Q000473:pathology; D003804:Dentin / Q000187:drug effects / Q000473:pathology; D004311:Double-Blind Method; D005459:Fluorides / Q000008:administration & dosage*; D006244:Hardness; D006801:Humans; D010710:Phosphates / Q000008:administration & dosage*; D013395:Sucrose / Q000008:administration & dosage; D013997:Time Factors; D017001:Tooth Demineralization / Q000517:prevention & control*; D014089:Tooth Remineralization; D014100:Toothpastes / Q000008:administration & dosage* / Q000494:pharmacology
chemical_list: D002327:Cariostatic Agents; D010710:Phosphates; D014100:Toothpastes; C012980:fluorophosphate; D013395:Sucrose; D005459:Fluorides