title: Protective effect of pterostilbene on testicular ischemia/reperfusion injury in rats.
abstract: OBJECTIVE
  To evaluate protective effect of pterostilbene against testicular ischemia/reperfusion (I/R) injury, which results in increased formation of oxidative stress, leading to testicular apoptosis and impaired spermatogenesis.
  
  
  METHODS
  Thirty two pubertal male Sprague-Dawley rats weighing 180-220g were selected and randomly divided into the following four groups: group A (normal control group), group B (sham-operated group), group C (induced I/R injury group), group D (induced I/R injury group receiving pterostilbene treatment). Johnsen's scores and mean seminiferous tubule diameters were evaluated for histopathologic assessment; germinal cell apoptosis was evaluated by the transferase dUTP nick end labeling (TUNEL) assay and immunohistochemistry for caspases. Malondialdehyde (MDA) levels were assessed as an indicator of oxidative stress and total antioxidant capacity (TAC) was measured.
  
  
  RESULTS
  Germ cell apoptosis and MDA level significantly increased whereas TAC significantly decreased in group C; moreover, abnormal morphology and impaired spermatogenesis were observed in group C. In contrast, treatment with pterostilbene inhibited lipid peroxidation and apoptosis induced by ROS and restored the antioxidant capacity in group D.
  
  
  CONCLUSIONS
  These results show that treatment with pterostilbene may be a promising therapy for testicular I/R injury.
authors.affiliation: The Institute for the 3Rs & Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Hyung Joon
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: The Institute for the 3Rs & Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Jae-Won
authors.initials: JW
authors.lastname: Lee
authors.affiliation: The Institute for the 3Rs & Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Bo Ram
authors.initials: BR
authors.lastname: Hwang
authors.affiliation: Department of Animal Science, Shingu College University, 2 Geumgwang-dong, Jungwon-gu, Seongnam-si, Gyeonggi-do 462-743, Republic of Korea.
authors.forename: Young-Ah
authors.initials: YA
authors.lastname: Lee
authors.affiliation: The Institute for the 3Rs & Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Jong-In
authors.initials: JI
authors.lastname: Kim
authors.affiliation: Department of Pet Science, Seojeong College University, Yongam-ri, Eunhyeon-m yeon, Yangju-si, Gyeonggi-do 482-777, Republic of Korea.
authors.forename: Yoon Ju
authors.initials: YJ
authors.lastname: Cho
authors.affiliation: Laboratory Animal Research Center, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Hyun Jhung
authors.initials: HJ
authors.lastname: Jhun
authors.affiliation: The Institute for the 3Rs & Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea. Electronic address: labvet@konkuk.ac.kr.
authors.forename: Jin Soo
authors.initials: JS
authors.lastname: Han
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D013086:Spermatic Cord Torsion / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D013267:Stilbenes / Q000494:pharmacology / Q000627:therapeutic use*; D013737:Testis / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D013267:Stilbenes; C107773:pterostilbene; D008315:Malondialdehyde
keywords: Antioxidant; Apoptosis; Caspases; Testicular torsion