title: Detoxification of acidic biorefinery waste liquor for production of high value amino acid.
abstract: The current study evaluates the detoxification of acid pretreatment liquor (APL) using adsorbent (ADS 400 & ADS 800) or ion-exchange (A-27MP & A-72MP) resins and its potential for amino acid production. The APL is generated as a by-product from the pretreatment of lignocellulosic biomass and is rich monomeric sugars as well as sugar degradation products (fermentation inhibitors) such as furfural and hydroxymethyl furfural (HMF). Of the four resins compared, ADS 800 removed approximately 85% and 60% of furfural and HMF, respectively. ADS 800 could be reused for up to six cycles after regeneration without losing its adsorption properties. The study was further extended by assessing the fermentability of detoxified APL for l-lysine production using wild and mutant strains of Corynebacterium glutamicum. The detoxified APL was superior to APL for l-lysine production.
authors.affiliation: Centre for Biofuels and Biotechnology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Industrial Estate P.O., Thiruvananthapuram 695019, India.
authors.forename: Meera
authors.initials: M
authors.lastname: Christopher
authors.affiliation: Centre for Biofuels and Biotechnology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Industrial Estate P.O., Thiruvananthapuram 695019, India.
authors.forename: Murali
authors.initials: M
authors.lastname: Anusree
authors.affiliation: Centre for Biofuels and Biotechnology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Industrial Estate P.O., Thiruvananthapuram 695019, India. Electronic address: anilaykara@gmail.com.
authors.forename: Anil K
authors.initials: AK
authors.lastname: Mathew
authors.affiliation: Centre for Biofuels and Biotechnology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Industrial Estate P.O., Thiruvananthapuram 695019, India.
authors.forename: K Madhavan
authors.initials: KM
authors.lastname: Nampoothiri
authors.affiliation: Centre for Biofuels and Biotechnology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Industrial Estate P.O., Thiruvananthapuram 695019, India.
authors.forename: Rajeev Kumar
authors.initials: RK
authors.lastname: Sukumaran
authors.affiliation: Centre for Biofuels and Biotechnology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Industrial Estate P.O., Thiruvananthapuram 695019, India.
authors.forename: Ashok
authors.initials: A
authors.lastname: Pandey
mesh_terms: D000327:Adsorption; D018533:Biomass; D001709:Biotechnology / Q000379:methods; D048230:Corynebacterium glutamicum / Q000254:growth & development / Q000378:metabolism; D005285:Fermentation; D005662:Furaldehyde / Q000031:analogs & derivatives / Q000378:metabolism; D008031:Lignin / Q000378:metabolism; D008239:Lysine / Q000096:biosynthesis*; D014865:Waste Disposal, Fluid / Q000379:methods*; D014866:Waste Products
chemical_list: D014866:Waste Products; C036909:lignocellulose; C008046:5-hydroxymethylfurfural; D008031:Lignin; D005662:Furaldehyde; D008239:Lysine
keywords: Adsorption; Biorefinery; Detoxification; Dilute acid pre-treatment; Lysine