title: Anti-Diabetic Activities of Gastrodia elata Blume Water Extracts Are Mediated Mainly by Potentiating Glucose-Stimulated Insulin Secretion and Increasing β-Cell Mass in Non-Obese Type 2 Diabetic Animals.
abstract: The brain is an important modulator of glucose metabolism, and is known to respond Gastrodia elata Blume water extract (GEB). Therefore, we examined whether long-term administration of GEB has hypoglycemic activity, and its action mechanism was explored in partially-pancreatectomized rats that exhibit similar characteristics as Asian type 2 diabetes, non-obese insulin-insufficient diabetes. The rats were provided high-fat diets supplemented with either of (1) 0.5% GEB (GEB-L), (2) 2% GEB (GEB-H), (3) 2% dextrin (control), or (4) 2% dextrin with rosiglitazone (20 mg/kg body weight; positive-control) for eight weeks. GEB dose-dependently improved hypothalamic insulin signaling, enhanced whole-body insulin sensitivity during hyperinsulinemic euglycemic clamp, and reduced hepatic glucose output in a hyperinsulinemic state. GEB dose-dependently increased the area under the curve of the serum insulin levels at the first and second phases during hyperglycemic clamp compared to the control, whereas the positive control had no effect. Insulin sensitivity during the hyperglycemic state also improved, dose-dependently, in response to GEB compared with that of the control, but was less than the positive control. GEB-H increased the mass of β-cells by potentiating proliferation and decreasing apoptosis. In conclusion, GEB could be a therapeutic agent for treating Asian type 2 diabetes.
authors.affiliation: Food Functional Research Division, Korean Food Research Institutes, Sungnam 463-746, Korea. yhj@kfri.re.kr.
authors.forename: Hye Jeong
authors.initials: HJ
authors.lastname: Yang
authors.affiliation: Food Functional Research Division, Korean Food Research Institutes, Sungnam 463-746, Korea. kmj@kfri.re.kr.
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Food Functional Research Division, Korean Food Research Institutes, Sungnam 463-746, Korea. dykwon@kfri.re.kr.
authors.forename: Dae Young
authors.initials: DY
authors.lastname: Kwon
authors.affiliation: Department of Food and Nutrition, Obesity/Diabetes Center, Hoseo University, Asan 336-795, Korea. tpfptm14@daum.net.
authors.forename: Da Sol
authors.initials: DS
authors.lastname: Kim
authors.affiliation: Department of Nanobiomechatronics, Hoseo University, Asan 336-795, Korea. tppoc002@naver.com.
authors.forename: Young Hyun
authors.initials: YH
authors.lastname: Lee
authors.affiliation: Department of Food and Nutrition, Obesity/Diabetes Center, Hoseo University, Asan 336-795, Korea. tjtnwls56@hanmail.net.
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Kim
authors.affiliation: Department of Food and Nutrition, Obesity/Diabetes Center, Hoseo University, Asan 336-795, Korea. smpark@hoseo.edu.|Department of Nanobiomechatronics, Hoseo University, Asan 336-795, Korea. smpark@hoseo.edu.
authors.forename: Sunmin
authors.initials: S
authors.lastname: Park
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose / Q000187:drug effects* / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism / Q000187:drug effects; D031668:Gastrodia / Q000737:chemistry*; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism; D007328:Insulin / Q000097:blood / Q000378:metabolism*; D007333:Insulin Resistance; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D010180:Pancreatectomy; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D017207:Rats, Sprague-Dawley; D012997:Solvents / Q000737:chemistry*; D013997:Time Factors; D014867:Water / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D007004:Hypoglycemic Agents; D007328:Insulin; D010936:Plant Extracts; D012997:Solvents; D014867:Water
keywords: Gastrodia elata Blume; insulin resistance; insulin secretagogues; type 2 diabetes; β-cell mass