title: Roles of endothelial A-type lamins in migration of T cells on and under endothelial layers.
abstract: Stiff nuclei in cell-dense microenvironments may serve as distinct biomechanical cues for cell migration, but such a possibility has not been tested experimentally. As a first step addressing this question, we altered nuclear stiffness of endothelial cells (ECs) by reducing the expression of A-type lamins using siRNA, and investigated the migration of T cells on and under EC layers. While most T cells crawling on control EC layers avoided crossing over EC nuclei, a significantly higher fraction of T cells on EC layers with reduced expression of A-type lamins crossed over EC nuclei. This result suggests that stiff EC nuclei underlying T cells may serve as "duro-repulsive" cues to direct T cell migration toward less stiff EC cytoplasm. During subendothelial migration under EC layers with reduced expression of A-type lamins, T cells made prolonged contact and substantially deformed EC nuclei, resulting in reduced speed and directional persistence. This result suggests that EC nuclear stiffness promotes fast and directionally persistent subendothelial migration of T cells by allowing minimum interaction between T cells and EC nuclei.
authors.affiliation: Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH) San 31, Hyoja-dong, Nam-Gu, Pohang, Gyeongbuk, 790-784, Korea.
authors.forename: Kwang Hoon
authors.initials: KH
authors.lastname: Song
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering (I-Bio), Pohang University of Science and Technology (POSTECH) San 31, Hyoja-dong, Nam-Gu, Pohang, Gyeongbuk, 790-784, Korea.
authors.forename: Jaehyun
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH) San 31, Hyoja-dong, Nam-Gu, Pohang, Gyeongbuk, 790-784, Korea.
authors.forename: HyoungJun
authors.initials: H
authors.lastname: Park
authors.affiliation: Division of Integrative Bioscience and Biotechnology (IBB), Pohang University of Science and Technology (POSTECH) San 31, Hyoja-dong, Nam-Gu, Pohang, Gyeongbuk, 790-784, Korea.
authors.forename: Hye Mi
authors.initials: HM
authors.lastname: Kim
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering (I-Bio), Pohang University of Science and Technology (POSTECH) San 31, Hyoja-dong, Nam-Gu, Pohang, Gyeongbuk, 790-784, Korea.
authors.forename: Jeehun
authors.initials: J
authors.lastname: Park
authors.affiliation: Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH) San 31, Hyoja-dong, Nam-Gu, Pohang, Gyeongbuk, 790-784, Korea.
authors.forename: Keon Woo
authors.initials: KW
authors.lastname: Kwon
authors.affiliation: Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH) San 31, Hyoja-dong, Nam-Gu, Pohang, Gyeongbuk, 790-784, Korea.|School of Interdisciplinary Bioscience and Bioengineering (I-Bio), Pohang University of Science and Technology (POSTECH) San 31, Hyoja-dong, Nam-Gu, Pohang, Gyeongbuk, 790-784, Korea.
authors.forename: Junsang
authors.initials: J
authors.lastname: Doh
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000502:physiology* / Q000648:ultrastructure; D034904:Lamin Type A / Q000378:metabolism / Q000502:physiology*; D008562:Membrane Glycoproteins; D051379:Mice; D013601:T-Lymphocytes / Q000166:cytology / Q000502:physiology*
chemical_list: D034904:Lamin Type A; D008562:Membrane Glycoproteins; C048022:substrate adhesion molecules