title: A large dataset of protein dynamics in the mammalian heart proteome.
abstract: Protein stability is a major regulatory principle of protein function and cellular homeostasis. Despite limited understanding on mechanisms, disruption of protein turnover is widely implicated in diverse pathologies from heart failure to neurodegenerations. Information on global protein dynamics therefore has the potential to expand the depth and scope of disease phenotyping and therapeutic strategies. Using an integrated platform of metabolic labeling, high-resolution mass spectrometry and computational analysis, we report here a comprehensive dataset of the in vivo half-life of 3,228 and the expression of 8,064 cardiac proteins, quantified under healthy and hypertrophic conditions across six mouse genetic strains commonly employed in biomedical research. We anticipate these data will aid in understanding key mitochondrial and metabolic pathways in heart diseases, and further serve as a reference for methodology development in dynamics studies in multiple organ systems.
authors.affiliation: The NIH Big Data to Knowledge (BD2K) Center of Excellence in Biomedical Computing at UCLA, Los Angeles, California 90095, USA.|Department of Physiology, University of California at Los Angeles, Los Angeles, California 90095, USA.
authors.forename: Edward
authors.initials: E
authors.lastname: Lau
authors.affiliation: The NIH Big Data to Knowledge (BD2K) Center of Excellence in Biomedical Computing at UCLA, Los Angeles, California 90095, USA.|Department of Physiology, University of California at Los Angeles, Los Angeles, California 90095, USA.|Department of Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Cao
authors.affiliation: The NIH Big Data to Knowledge (BD2K) Center of Excellence in Biomedical Computing at UCLA, Los Angeles, California 90095, USA.|Department of Physiology, University of California at Los Angeles, Los Angeles, California 90095, USA.
authors.forename: Dominic C M
authors.initials: DC
authors.lastname: Ng
authors.affiliation: The NIH Big Data to Knowledge (BD2K) Center of Excellence in Biomedical Computing at UCLA, Los Angeles, California 90095, USA.|Department of Physiology, University of California at Los Angeles, Los Angeles, California 90095, USA.
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Bleakley
authors.affiliation: The NIH Big Data to Knowledge (BD2K) Center of Excellence in Biomedical Computing at UCLA, Los Angeles, California 90095, USA.|Department of Physiology, University of California at Los Angeles, Los Angeles, California 90095, USA.|Department of Bioinformatics, University of California at Los Angeles, Los Angeles, California 90095, USA.
authors.forename: T Umut
authors.initials: TU
authors.lastname: Dincer
authors.affiliation: The NIH Big Data to Knowledge (BD2K) Center of Excellence in Biomedical Computing at UCLA, Los Angeles, California 90095, USA.|Department of Sage Bionetworks, Seattle, Washignton 98109, USA.
authors.forename: Brian M
authors.identifier: http://orcid.org/0000-0002-2412-6826
authors.initials: BM
authors.lastname: Bot
authors.affiliation: The NIH Big Data to Knowledge (BD2K) Center of Excellence in Biomedical Computing at UCLA, Los Angeles, California 90095, USA.|Department of Physiology, University of California at Los Angeles, Los Angeles, California 90095, USA.
authors.forename: Ding
authors.initials: D
authors.lastname: Wang
authors.affiliation: The NIH Big Data to Knowledge (BD2K) Center of Excellence in Biomedical Computing at UCLA, Los Angeles, California 90095, USA.|Department of Physiology, University of California at Los Angeles, Los Angeles, California 90095, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Liem
authors.affiliation: The NIH Big Data to Knowledge (BD2K) Center of Excellence in Biomedical Computing at UCLA, Los Angeles, California 90095, USA.|Department of Physiology, University of California at Los Angeles, Los Angeles, California 90095, USA.|Department of Bioinformatics, University of California at Los Angeles, Los Angeles, California 90095, USA.
authors.forename: Maggie P Y
authors.initials: MP
authors.lastname: Lam
authors.affiliation: Department of Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Junbo
authors.initials: J
authors.lastname: Ge
authors.affiliation: The NIH Big Data to Knowledge (BD2K) Center of Excellence in Biomedical Computing at UCLA, Los Angeles, California 90095, USA.|Department of Physiology, University of California at Los Angeles, Los Angeles, California 90095, USA.|Department of Bioinformatics, University of California at Los Angeles, Los Angeles, California 90095, USA.|Department of Medicine,University of California at Los Angeles, Los Angeles, California 90095, USA.
authors.forename: Peipei
authors.initials: P
authors.lastname: Ping
mesh_terms: D000818:Animals; D006332:Cardiomegaly / Q000378:metabolism; D004734:Energy Metabolism; D008322:Mammals; D051379:Mice; D008929:Mitochondria, Heart / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D040901:Proteomics; D013045:Species Specificity
chemical_list: D009124:Muscle Proteins