title: Direct and sensitive determination of glyphosate and aminomethylphosphonic acid in environmental water samples by high performance liquid chromatography coupled to electrospray tandem mass spectrometry.
abstract: A novel method based on high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) was developed for the sensitive determination of glyphosate and its major degradation product, AMPA in environmental water samples. The method involves the use of MS compatible mobile phases (0.1% formic acid in water and acetonitrile) for HPLC and direct analysis of water samples without sample derivatization. The method has been validated in different types of water matrices (drinking, surface and groundwater) by accuracy and precision studies with samples spiked at 0.1, 7.5 and 90 ppb. All mean accuracy values ranged from 85% to 112% for glyphosate and AMPA using both primary and secondary quantitative ion transitions (RSD ≤ 10%). Moreover, both primary and secondary ion transitions for glyphosate and AMPA can achieve the quantitation limits at 0.1 ppb. The linear dynamic range of the calibration curves were from 0.1 to 100 ppb for each analyte at each ion transitions with correlation coefficient higher than 0.997.
authors.affiliation: Monsanto Company, St Louis, MO, United States; University of Texas at Arlington, Arlington, TX, United States. Electronic address: hongyue.guo@mavs.uta.edu.
authors.forename: Hongyue
authors.initials: H
authors.lastname: Guo
authors.affiliation: Monsanto Company, St Louis, MO, United States. Electronic address: lsriter@monsanto.com.
authors.forename: Leah S
authors.initials: LS
authors.lastname: Riter
authors.affiliation: Monsanto Company, St Louis, MO, United States. Electronic address: chad.e.wujcik@monsanto.com.
authors.forename: Chad E
authors.initials: CE
authors.lastname: Wujcik
authors.affiliation: University of Texas at Arlington, Arlington, TX, United States. Electronic address: sec4dwa@uta.edu.
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Armstrong
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004784:Environmental Monitoring / Q000379:methods*; D005998:Glycine / Q000031:analogs & derivatives* / Q000032:analysis; D007555:Isoxazoles; D057230:Limit of Detection; D063065:Organophosphonates / Q000032:analysis*; D053719:Tandem Mass Spectrometry; D013777:Tetrazoles; D014867:Water / Q000737:chemistry*; D000097797:Glyphosate
chemical_list: D005998:Glycine; D007555:Isoxazoles; D063065:Organophosphonates; D013777:Tetrazoles; D014867:Water; D000097797:Glyphosate; C418233:2-amino-3-(3-hydrox-5(2-methyl-2H-tetrazol-5-yl)isoxazol-4-yl)propionic acid
keywords: AMPA; Direct analysis; Environmental water samples; Glyphosate; High performance liquid chromatography; Sensitivity; Tandem mass spectrometry