title: Roles of Long Non-Coding RNA CCAT2 in Cervical Cancer Cell Growth and Apoptosis.
abstract: BACKGROUND
  This study aimed to analyze the potential function of lncRNA CCAT2 in cervical cancer cell proliferation and apoptosis.
  
  
  METHODS
  Expression level of CCAT2 in cervical cancer cell lines (HeLa, CaSki, and SiHa) was detected by quantitative real-time PCR. CCAT2 knockdown was established by transfecting siRNA into human cervical cancer cells. Its effects on cell proliferation were studied using cell-counting kit-8 assay. The effect of CCAT2 on cervical cancer cells cycle and apoptosis was assessed by flow cytometry assay.
  
  
  RESULTS
  CCK8 assay showed that CCAT2 knockdown inhibited cell proliferation in HeLa, CaSki, and SiHa cells. The flow cytometry confirmed the results that knockdown of CCAT2 could induce cervical cancer cells cycle G1 phase arrestment and trigger the cells apoptosis.
  
  
  CONCLUSIONS
  LncRNA CCAT2 promoted the proliferation and survival of cervical cancer cells.
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China (mainland).
authors.forename: Le
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China (mainland).
authors.forename: Lixu
authors.initials: L
authors.lastname: Jin
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China (mainland).
authors.forename: Wenmiao
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China (mainland).
authors.forename: Lifang
authors.initials: L
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology*
chemical_list: D062085:RNA, Long Noncoding; C000609264:long non-coding RNA CCAT2, human