title: Chemical constituents from rhizome of Anemone amurensis.
abstract: Phytochemical investigation of the 70% EtOH extract of the rhizome of Anemone amurensis led to the isolation of two new oleanane-type triterpenoid saponins 1 and 2. Their structures were elucidated on the basis of chemical and spectral analysis, including 1D, 2D NMR data, and HR-ESI-MS. Compounds 1 and 2 were tested for cytotoxicities against two human cancer cell lines (A549 and Hep-G2). Compound 2 showed potent cytotoxicity with IC50 values of 38.53 and 66.17 μM, respectively, while compound 1 with IC50 > 100 μM.
authors.affiliation: a School of Traditional Chinese Materia Medica , Shenyang Pharmaceutical University , Shenyang 110016 , China.
authors.forename: Chong-Ning
authors.initials: CN
authors.lastname: Lv
authors.affiliation: a School of Traditional Chinese Materia Medica , Shenyang Pharmaceutical University , Shenyang 110016 , China.
authors.forename: Yan-Jiao
authors.initials: YJ
authors.lastname: Li
authors.affiliation: b Department of Science of Chinese Medicinal Material Resources, School of Traditional Chinese Materia Medica , Shenyang Pharmaceutical University , Shenyang 110016 , China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: a School of Traditional Chinese Materia Medica , Shenyang Pharmaceutical University , Shenyang 110016 , China.
authors.forename: Ru-Lan
authors.initials: RL
authors.lastname: Qin
authors.affiliation: a School of Traditional Chinese Materia Medica , Shenyang Pharmaceutical University , Shenyang 110016 , China.
authors.forename: Tian-Li
authors.initials: TL
authors.lastname: Lei
authors.affiliation: a School of Traditional Chinese Materia Medica , Shenyang Pharmaceutical University , Shenyang 110016 , China.
authors.forename: Jin-Cai
authors.initials: JC
authors.lastname: Lu
mesh_terms: D031883:Anemone / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry; D004354:Drug Screening Assays, Antitumor; D056945:Hep G2 Cells; D006801:Humans; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D009828:Oleanolic Acid / Q000031:analogs & derivatives; D010936:Plant Extracts / Q000737:chemistry; D027343:Rhizome / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D012503:Saponins; C413246:oleanane; D009828:Oleanolic Acid
keywords: Anemone amurensis; NMR; cytotoxic activity; triterpenoid saponins