title: The Effect on Cognition of Mitochondrial Respiratory System Proteins in Peripheral Blood Mononuclear Cells in the Course of Lung Cancer.
abstract: Peripheral blood mononuclear cells (PBMC) represent an easily available population of cells for the studies on remote effects of lung cancer. NADH dehydrogenase (ubiquinone) Fe-S protein-1 (Ndufs1), a marker of mitochondrial complex I, and mitochondrially encoded cytochrome c oxidase 1 (MTCO1), a marker of complex IV, may participate in cognitive decline during the course of lung cancer. In this study, Ndufs1 and MTCO1 expression in PBMC was evaluated by means of ELISA in 80 lung cancer patients. Mini-Mental State Examination (MMSE) were conducted Trail Making Tests (TMT-A and TMT-B) at baseline and after the 6 months' follow-up. Autoantibodies were identified by means of indirect immunofluorescence and line blot. We found that enhanced levels of Ndufs1 in PBMC were related to impaired cognitive performance; TMT-A of 13.6 ± 3.1 s and TMT-B of 162.5 ± 46.4 s compared with 8.6 ± 4.5 s (p = 0.003) and 124.8 ± 51.8 s (p < 0.05), respectively, in the case of low Ndufs-1 levels. The Ndufs1 expression at baseline was associated with MMSE - τb (Kendall's tau-b) = -0.31; p = 0.024; TMT-A - τb = 0.30; p = 0.001), and TMT-B - τb = 0.199; p = 0.012) after the 6 months' follow-up. Higher MTCO1 expression was accompanied by worse TMT-A results than in case of inhibited MTCO1; 11.1 ± 5.8 s vs. 8.5 ± 4.1 s; respectively; p = 0.048. MTCO1 expression was correlated with TMT-A results (τb = 0.17; p = 0.034) at baseline. We conclude that stimulation of PBMC mitochondrial function in lung cancer patients is associated with cognitive impairment. Mitochondrial dysfunction in PBMC may reflect cytotoxicity responsible for neurological deficits.
authors.affiliation: Department of Neurochemistry and Neuropathology, Poznan University of Medical Sciences, 49 Przybyszewskiego St, 60-355, Poznan, Poland. swami622@gmail.com.|Neuroimmunological Unit, The Mossakowski Medical Research Center of the Polish Academy of Sciences, Poznan, Poland. swami622@gmail.com.
authors.forename: S
authors.initials: S
authors.lastname: Michalak
authors.affiliation: Department of Neurochemistry and Neuropathology, Poznan University of Medical Sciences, 49 Przybyszewskiego St, 60-355, Poznan, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Rybacka-Mossakowska
authors.affiliation: Cancer Center, Poznan, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Gazdulska
authors.affiliation: Lung Diseases Center, Poznan, Poland.
authors.forename: I
authors.initials: I
authors.lastname: Gołda-Gocka
authors.affiliation: Department of Oncology, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: R
authors.initials: R
authors.lastname: Ramlau
mesh_terms: D000230:Adenocarcinoma / Q000150:complications / Q000473:pathology / Q000523:psychology; D014408:Biomarkers, Tumor / Q000097:blood*; D018287:Carcinoma, Large Cell / Q000150:complications / Q000473:pathology / Q000523:psychology; D002289:Carcinoma, Non-Small-Cell Lung / Q000150:complications / Q000473:pathology / Q000523:psychology; D002294:Carcinoma, Squamous Cell / Q000150:complications / Q000473:pathology / Q000523:psychology; D003072:Cognition Disorders / Q000097:blood / Q000175:diagnosis* / Q000209:etiology / Q000523:psychology; D003576:Electron Transport Complex IV / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008175:Lung Neoplasms / Q000150:complications* / Q000473:pathology / Q000523:psychology; D008297:Male; D008875:Middle Aged; D009245:NADH Dehydrogenase / Q000097:blood*; D009367:Neoplasm Staging; D009483:Neuropsychological Tests; D011379:Prognosis; D011597:Psychomotor Performance; D055752:Small Cell Lung Carcinoma / Q000150:complications / Q000473:pathology / Q000523:psychology; D014145:Trail Making Test
chemical_list: D014408:Biomarkers, Tumor; C487793:NDUFS1 protein, human; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV; C581860:cytochrome c oxidase subunit I, human
keywords: Cognitive functions; Memory; Mitochondrial complex; Pulmonary neoplasms