title: In Vivo Quantitative Assessment of Myocardial Structure, Function, Perfusion and Viability Using Cardiac Micro-computed Tomography.
abstract: The use of Micro-Computed Tomography (MicroCT) for in vivo studies of small animals as models of human disease has risen tremendously due to the fact that MicroCT provides quantitative high-resolution three-dimensional (3D) anatomical data non-destructively and longitudinally. Most importantly, with the development of a novel preclinical iodinated contrast agent called eXIA160, functional and metabolic assessment of the heart became possible. However, prior to the advent of commercial MicroCT scanners equipped with X-ray flat-panel detector technology and easy-to-use cardio-respiratory gating, preclinical studies of cardiovascular disease (CVD) in small animals required a MicroCT technologist with advanced skills, and thus were impractical for widespread implementation. The goal of this work is to provide a practical guide to the use of the high-speed Quantum FX MicroCT system for comprehensive determination of myocardial global and regional function along with assessment of myocardial perfusion, metabolism and viability in healthy mice and in a cardiac ischemia mouse model induced by permanent occlusion of the left anterior descending coronary artery (LAD).
authors.affiliation: Department of Genetics, Erasmus MC, Rotterdam; Department of Experimental Cardiology, Erasmus MC, Rotterdam.
authors.forename: Elza
authors.initials: E
authors.lastname: van Deel
authors.affiliation: Department of Genetics, Erasmus MC, Rotterdam.
authors.forename: Yanto
authors.initials: Y
authors.lastname: Ridwan
authors.affiliation: Department of Genetics, Erasmus MC, Rotterdam.
authors.forename: J Nicole
authors.initials: JN
authors.lastname: van Vliet
authors.affiliation: PerkinElmer, Inc.
authors.forename: Sasha
authors.initials: S
authors.lastname: Belenkov
authors.affiliation: Department of Genetics, Erasmus MC, Rotterdam; Department of Vascular Surgery, Erasmus MC, Rotterdam; Department of Radiation Oncology, Erasmus MC, Rotterdam; j.essers@erasmusmc.nl.
authors.forename: Jeroen
authors.initials: J
authors.lastname: Essers
mesh_terms: D000818:Animals; D003287:Contrast Media; D003324:Coronary Artery Disease / Q000000981:diagnostic imaging; D004195:Disease Models, Animal; D006321:Heart / Q000000981:diagnostic imaging / Q000503:physiopathology; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D017202:Myocardial Ischemia / Q000000981:diagnostic imaging* / Q000473:pathology / Q000503:physiopathology; D009206:Myocardium / Q000473:pathology*; D018487:Ventricular Dysfunction, Left / Q000000981:diagnostic imaging / Q000473:pathology / Q000503:physiopathology; D055114:X-Ray Microtomography / Q000379:methods*
chemical_list: D003287:Contrast Media