title: Transcriptomic profiling of Arabidopsis gene expression in response to varying micronutrient zinc supply.
abstract: Deficiency of the micronutrient zinc is a widespread condition in agricultural soils, causing a negative impact on crop quality and yield. Nevertheless, there is an insufficient knowledge on the regulatory and molecular mechanisms underlying the plant response to inadequate zinc nutrition [1]. This information should contribute to the development of plant-based solutions with improved nutrient-use-efficiency traits in crops. Previously, the transcription factors bZIP19 and bZIP23 were identified as essential regulators of the response to zinc deficiency in Arabidopsis thaliana [2]. A microarray experiment comparing gene expression between roots of wild-type and the mutant bzip19 bzip23, exposed to zinc deficiency, led to the identification of differentially expressed genes related with zinc homeostasis, namely its transport and plant internal translocation [2]. Here, we provide the detailed methodology, bioinformatics analysis and quality controls related to the microarray gene expression profiling published by Assunção and co-workers [2]. Most significantly, the present dataset comprises new experimental variables, including analysis of shoot tissue, and zinc sufficiency and excess supply. Thus, it expands from 8 to 42 microarrays hybridizations, which have been deposited at the Gene Expression Omnibus (GEO) under the accession number GSE77286. Overall, it provides a resource for research on the molecular basis and regulatory events of the plant response to zinc supply, emphasizing the importance of Arabidopsis bZIP19 and bZIP23 transcription factors.
authors.affiliation: CIBIO-InBIO, Research Network in Biodiversity and Evolutionary Biology, Universidade do Porto, Campus Agrário de Vairão, 4485-661 Vairão, Portugal.
authors.forename: Herlânder
authors.initials: H
authors.lastname: Azevedo
authors.affiliation: CIBIO-InBIO, Research Network in Biodiversity and Evolutionary Biology, Universidade do Porto, Campus Agrário de Vairão, 4485-661 Vairão, Portugal.
authors.forename: Sarah Gaspar
authors.initials: SG
authors.lastname: Azinheiro
authors.affiliation: CIBIO-InBIO, Research Network in Biodiversity and Evolutionary Biology, Universidade do Porto, Campus Agrário de Vairão, 4485-661 Vairão, Portugal.
authors.forename: Antonio
authors.initials: A
authors.lastname: Muñoz-Mérida
authors.affiliation: Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871, Denmark.
authors.forename: Pedro Humberto
authors.initials: PH
authors.lastname: Castro
authors.affiliation: Max Planck Institute for Plant Breeding ADIS/DNA Core Facility, D-50829 Cologne, Germany.
authors.forename: Bruno
authors.initials: B
authors.lastname: Huettel
authors.affiliation: Laboratory of Genetics, Wageningen University, 6708 PB Wageningen, The Netherlands.
authors.forename: Mark G M
authors.initials: MG
authors.lastname: Aarts
authors.affiliation: CIBIO-InBIO, Research Network in Biodiversity and Evolutionary Biology, Universidade do Porto, Campus Agrário de Vairão, 4485-661 Vairão, Portugal; Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871, Denmark.
authors.forename: Ana G L
authors.initials: AG
authors.lastname: Assunção
keywords: Arabidopsis; Microarray; Micronutrient; Zinc deficiency; bZIP