title: Enabling Metabolomics Based Biomarker Discovery Studies Using Molecular Phenotyping of Exosome-Like Vesicles.
abstract: Identification of sensitive and specific biomarkers with clinical and translational utility will require smart experimental strategies that would augment expanding the breadth and depth of molecular measurements within the constraints of currently available technologies. Exosomes represent an information rich matrix to discern novel disease mechanisms that are thought to contribute to pathologies such as dementia and cancer. Although proteomics and transcriptomic studies have been reported using Exosomes-Like Vesicles (ELVs) from different sources, exosomal metabolome characterization and its modulation in health and disease remains to be elucidated. Here we describe methodologies for UPLC-ESI-MS based small molecule profiling of ELVs from human plasma and cell culture media. In this study, we present evidence that indeed ELVs carry a rich metabolome that could not only augment the discovery of low abundance biomarkers but may also help explain the molecular basis of disease progression. This approach could be easily translated to other studies seeking to develop predictive biomarkers that can subsequently be used with simplified targeted approaches.
authors.affiliation: Biomedical Research Group in Ginecology, Hospital Universitari Vall d'Hebron, Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Altadill
authors.affiliation: Biomedical Research Group in Ginecology, Hospital Universitari Vall d'Hebron, Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain.
authors.forename: Irene
authors.initials: I
authors.lastname: Campoy
authors.affiliation: Biomedical Research Group in Ginecology, Hospital Universitari Vall d'Hebron, Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain.
authors.forename: Lucia
authors.initials: L
authors.lastname: Lanau
authors.affiliation: Departments of Oncology and Biochemistry, Molecular and Cellular Biology, Lombardi Comprehensive Cancer Center at Georgetown University Medical Center, Washington, D.C., United States of America.
authors.forename: Kirandeep
authors.initials: K
authors.lastname: Gill
authors.affiliation: Institut d'Investigació Biomedica de Bellvitge (IDIBELL), Barcelona, Spain.
authors.forename: Marina
authors.initials: M
authors.lastname: Rigau
authors.affiliation: Gynecological Department, Vall Hebron University Hospital, Universitat Autònoma de Barcelona, Barcelona, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Gil-Moreno
authors.affiliation: Institut d'Investigació Biomedica de Bellvitge (IDIBELL), Barcelona, Spain.
authors.forename: Jaume
authors.initials: J
authors.lastname: Reventos
authors.affiliation: Departments of Oncology and Biochemistry, Molecular and Cellular Biology, Lombardi Comprehensive Cancer Center at Georgetown University Medical Center, Washington, D.C., United States of America.
authors.forename: Stephen
authors.initials: S
authors.lastname: Byers
authors.affiliation: Biomedical Research Group in Ginecology, Hospital Universitari Vall d'Hebron, Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain.|Department of Pathology and Molecular Genetics/Oncologic Pathology Group, Hospital Universitari Arnau de Vilanova, Universitat de Lleida, IRBLleida, Lleida, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Colas
authors.affiliation: Departments of Oncology and Biochemistry, Molecular and Cellular Biology, Lombardi Comprehensive Cancer Center at Georgetown University Medical Center, Washington, D.C., United States of America.
authors.forename: Amrita K
authors.initials: AK
authors.lastname: Cheema
mesh_terms: D015415:Biomarkers / Q000097:blood*; D016022:Case-Control Studies; D002460:Cell Line; D003470:Culture Media; D055354:Exosomes / Q000378:metabolism*; D006801:Humans; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D015999:Multivariate Analysis; D053758:Nanoparticles; D010641:Phenotype; D015203:Reproducibility of Results; D016212:Transforming Growth Factor beta / Q000494:pharmacology
chemical_list: D015415:Biomarkers; D003470:Culture Media; D016212:Transforming Growth Factor beta