title: Bioremediation technologies for polluted seawater sampled after an oil-spill in Taranto Gulf (Italy): A comparison of biostimulation, bioaugmentation and use of a washing agent in microcosm studies.
abstract: One of the main challenges of bioremediation is to define efficient protocols having a low environmental impact. We have investigated the effect of three treatments in oily-seawater after a real oil-spill occurred in the Gulf of Taranto (Italy). Biostimulation with inorganic nutrients allowed the biodegradation of the 73±2.4% of hydrocarbons, bioaugmentation with a selected hydrocarbonoclastic consortium consisting of Alcanivorax borkumensis, Alcanivorax dieselolei, Marinobacter hydrocarbonoclasticus, Cycloclasticus sp. 78-ME and Thalassolituus oleivorans degraded 79±3.2%, while the addition of nutrients and a washing agent has allowed the degradation of the 69±2.6%. On the other hand, microbial community was severely affected by the addition of the washing agent and the same product seemed to inhibit the growth of the majority of strains composing the selected consortium at the tested concentration. The use of dispersant should be accurately evaluated also considering its effect on the principal actors of biodegradation.
authors.affiliation: Institute for Coastal Marine Environment CNR, Sp.ta S. Raineri 86, 98122 Messina, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Crisafi
authors.affiliation: Institute for Coastal Marine Environment CNR, Sp.ta S. Raineri 86, 98122 Messina, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Genovese
authors.affiliation: Institute for Coastal Marine Environment CNR, Sp.ta S. Raineri 86, 98122 Messina, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Smedile
authors.affiliation: Institute for Coastal Marine Environment CNR, Sp.ta S. Raineri 86, 98122 Messina, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Russo
authors.affiliation: Institute for Coastal Marine Environment CNR, Sp.ta S. Raineri 86, 98122 Messina, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Catalfamo
authors.affiliation: Institute for Coastal Marine Environment CNR, Sp.ta S. Raineri 86, 98122 Messina, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Yakimov
authors.affiliation: Institute for Coastal Marine Environment CNR, Sp.ta S. Raineri 86, 98122 Messina, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Giuliano
authors.affiliation: Institute for Coastal Marine Environment CNR, Sp.ta S. Raineri 86, 98122 Messina, Italy. Electronic address: renata.denaro@iamc.cnr.it.
authors.forename: R
authors.initials: R
authors.lastname: Denaro
mesh_terms: D001419:Bacteria / Q000378:metabolism; D001673:Biodegradation, Environmental; D052918:Environmental Restoration and Remediation / Q000379:methods*; D006838:Hydrocarbons / Q000378:metabolism; D007558:Italy; D009821:Oils; D010578:Petroleum / Q000032:analysis / Q000378:metabolism*; D059392:Petroleum Pollution; D012623:Seawater / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism*
chemical_list: D006838:Hydrocarbons; D009821:Oils; D010578:Petroleum; D014874:Water Pollutants, Chemical
keywords: Bioremediation; Hydrocarbonoclastic bacteria; Marine environment; Oil pollution; Washing agent