title: Pharmacodynamics of aminoglycosides and tetracycline derivatives against Japanese encephalitis virus.
abstract: OBJECTIVE
  To explore the antiviral activity of antibiotic compounds, mainly aminoglycosides and tetracyclines against Japanese encephalitis virus (JEV) induced infection in vitro.
  
  
  METHODS
  Antiviral activity were evaluated against JEV using cytopathic effect inhibition assay, virus yield reduction assay, caspase 3 level, extracellular viral detection by antigen capture ELISA and viral RNA levels.
  
  
  RESULTS
  JEV induced cytopathic effect along with reduction of viral progeny plaque formation indicated antiviral potential of the compounds suggesting that antibiotics had broad spectrum activity. Doxycycline and kanamycin administration in dose dependent manner declined viral RNA replication.
  
  
  CONCLUSIONS
  The present study shows kanamycin and doxycycline can affect virion structure and alter replication causing inhibition of JEV induced pathogenesis in vitro.
authors.affiliation: Regional Medical Research Centre, ICMR (NE Region), Dibrugarh 786001, Assam, India.
authors.forename: Rashmee
authors.initials: R
authors.lastname: Topno
authors.affiliation: Regional Medical Research Centre, ICMR (NE Region), Dibrugarh 786001, Assam, India. Electronic address: sirajkhanicmr@gmail.com.
authors.forename: Siraj Ahmed
authors.initials: SA
authors.lastname: Khan
authors.affiliation: Regional Medical Research Centre, ICMR (NE Region), Dibrugarh 786001, Assam, India.
authors.forename: Pritom
authors.initials: P
authors.lastname: Chowdhury
authors.affiliation: Regional Medical Research Centre, ICMR (NE Region), Dibrugarh 786001, Assam, India.
authors.forename: Jagadish
authors.initials: J
authors.lastname: Mahanta
keywords: 2 DOS aminoglycoside; Doxycycline; Japanese encephalitis virus; Kanamycin