title: [ROLE OF CASPASE-3 IN REGULATION OF THE CONTENT OF THE AMYLOID-DEGRADING NEUROPEPTIDASE NEPRILYSIN IN THE CORTEX OF RATS AFTER HYPOXIA].
abstract: Analysis of the effect of a caspase-3 inhibitor on the content of the amyloid-degrading neuropeptidase neprilysin (NEP) in the cortex of rats subjected to prenatal hypoxia (7% O2, 3 h) on the 14-th day of the embryonic development (E14) was performed. It was found that rats subjected to prenatal hypoxia on days 20-30 after birth have an increased content and activity of caspase-3 with reduced levels of NEP and of the C-terminal fragment of the amyloid precursor protein (AICD) regulating NEP expression. In hypoxic animals 3 days after a single injection of a caspase inhibitor (i. v., Ac-DEVD-CHO, P20) the content of AICD and NEP was found to be increased up to the levels observed in control rats. The data obtained suggest that the increase of caspase-3 enzyme activity could affect NEP expression via proteolytic degradation of its transcription factor AICD. These data for the first time demonstrate the role of caspases in AICD-dependent regulation of NEP production in the brain of mammals under hypoxic conditions.
authors.forename: D I
authors.initials: DI
authors.lastname: Kozlova
authors.forename: D S
authors.initials: DS
authors.lastname: Vasylev
authors.forename: N M
authors.initials: NM
authors.lastname: Dubrovskaya
authors.forename: N N
authors.initials: NN
authors.lastname: Nalivaeva
authors.forename: N L
authors.initials: NL
authors.lastname: Tumanova
authors.forename: I A
authors.initials: IA
authors.lastname: Zhuravin
mesh_terms: D016564:Amyloid beta-Protein Precursor / Q000378:metabolism; D000818:Animals; D053148:Caspase 3 / Q000096:biosynthesis*; D002540:Cerebral Cortex / Q000201:enzymology* / Q000473:pathology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D002534:Hypoxia, Brain / Q000201:enzymology* / Q000473:pathology; D015260:Neprilysin / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016564:Amyloid beta-Protein Precursor; C505410:Casp3 protein, rat; D053148:Caspase 3; D015260:Neprilysin