title: Facile purification and click labeling with 2-[18F]fluoroethyl azide using solid phase extraction cartridges.
abstract: A facile method was developed to purify 2-[18F]fluoroethyl azide ([18F]FEA) using a C18 cartridge and an Oasis®HLB cartridge in series, in which [18F]FEA was exclusively trapped on the HLB cartridge. [18F]FEA can be eluted for reactions in solution; alternatively click labeling can be carried out on the HLB cartridge itself by loading an alkyne substrate and copper (I) catalyst dissolved in DMF onto the cartridge. This solid phase extraction methodology for purification and click labeling with [18F]FEA, either in solution or on the cartridge, is safe, simple, reproducible in high yield, and compatible with automated synthesis of18F-labeled PET tracers.
authors.affiliation: Mallinckrodt Institute of Radiology, Washington University School of Medicine, 510 S. Kingshighway Blvd., St Louis, MO 63110, USA.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhou
authors.affiliation: Mallinckrodt Institute of Radiology, Washington University School of Medicine, 510 S. Kingshighway Blvd., St Louis, MO 63110, USA.
authors.forename: Wenhua
authors.initials: W
authors.lastname: Chu
authors.affiliation: Mallinckrodt Institute of Radiology, Washington University School of Medicine, 510 S. Kingshighway Blvd., St Louis, MO 63110, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Peng
authors.affiliation: Mallinckrodt Institute of Radiology, Washington University School of Medicine, 510 S. Kingshighway Blvd., St Louis, MO 63110, USA.
authors.forename: Jonathan
authors.initials: J
authors.lastname: McConathy
authors.affiliation: Mallinckrodt Institute of Radiology, Washington University School of Medicine, 510 S. Kingshighway Blvd., St Louis, MO 63110, USA.
authors.forename: Robert H
authors.initials: RH
authors.lastname: Mach
authors.affiliation: Department of Chemistry, University of Illinois, Urbana, IL 61801, USA.
authors.forename: John A
authors.initials: JA
authors.lastname: Katzenellenbogen
keywords: 2-Fluoroethyl azide; Click chemistry; Fluorine-18; Positron emission tomography; Radiolabeling; Solid phase extraction