title: CYP24A1 is a potential biomarker for the progression and prognosis of human colorectal cancer.
abstract: Our study aims to fully evaluate clinicopathological and prognostic values of CYP24A1 in colorectal cancer (CRC) patients. Tissue microarrays of formalin-fixed and paraffin-embedded tumor samples and matched adjacent nontumor colorectal tissues from 99 CRC patients were studied for CYP24A1 protein expression by immunohistochemistry. Messenger RNA expression of CYP24A1 was further evaluated by quantitative real-time polymerase chain reaction in 12 pairs of fresh frozen CRC samples. CYP24A1 expression was significantly higher in CRC tissues compared to corresponding noncancerous tissues. The expression of CYP24A1 protein in CRC was correlated with the depth of tumor invasion (P = .000), lymph node metastasis (P = .030), venous permeation (P = .016), and overall survival (P = .008). A Kaplan-Meier analysis of the CRC patients with high CYP24A1 expression showed significantly reduced overall survival and disease-free survival compared to the patients with low expression (P = 0.026 and .009). A prognostic significance of CYP24A1 was also found in the subgroup of venous permeation condition classification. A multivariate Cox regression analysis showed that CYP24A1 expression was an independent prognostic factor for CRC recurrence (P = .032). In conclusion, CYP24A1 expression is closely associated with CRC progression, and it might be a novel prognostic biomarker for CRC.
authors.affiliation: Department of Pathology, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province 130033, China; Department of Nutrition and Food Hygiene, School of Public Health, Jilin University, Changchun, Jilin Province 130021, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Radiological Health, Occupation Disease Prevention and Control Center of Jilin Province, Changchun, Jilin Province 130021, China.
authors.forename: Chuanwen
authors.initials: C
authors.lastname: Wang
authors.affiliation: Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province 130033, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Hao
authors.affiliation: Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province 130033, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Sun
authors.affiliation: Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province 130033, China.
authors.forename: Yuqian
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Pathology, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province 130033, China.
authors.forename: Tie
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Pathology, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province 130033, China. Electronic address: congxl888@hotmail.com.
authors.forename: Xianling
authors.initials: X
authors.lastname: Cong
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Jilin University, Changchun, Jilin Province 130021, China. Electronic address: liuyajlu@163.com.
authors.forename: Ya
authors.initials: Y
authors.lastname: Liu
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D016009:Chi-Square Distribution; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000401:mortality / Q000473:pathology / Q000628:therapy; D018450:Disease Progression; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D009364:Neoplasm Recurrence, Local; D011237:Predictive Value of Tests; D016016:Proportional Hazards Models; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012307:Risk Factors; D013997:Time Factors; D046888:Tissue Array Analysis; D016896:Treatment Outcome; D015854:Up-Regulation; D065668:Vitamin D3 24-Hydroxylase / Q000032:analysis* / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; C585549:CYP24A1 protein, human; D065668:Vitamin D3 24-Hydroxylase
keywords: CYP24A1; Colorectal cancer; Immunohistochemistry; Oncogene; Prognosis; Tissue microarray