title: Hyperspectral imaging techniques for the characterization of Haematococcus pluvialis (Chlorophyceae).
abstract: A hyperspectral imaging camera was combined with a bright-field microscope to investigate the intracellular distribution of pigments in cells of the green microalga Haematococcus pluvialis, a synonym for H. lacustris (Chlorophyceae). We applied multivariate curve resolution to the hyperspectral image data to estimate the pigment contents in culture and revealed that the predicted values were consistent with actual measurements obtained from extracted pigments. Because it was possible to estimate pigment contents in every pixel, the intracellular distribution of the pigments was investigated during various life-cycle stages. Astaxanthin was localized specifically at the eyespot of zoospores in early culture stages. Then, it became widely distributed in cells, but subsequently localized differently than the chl. Integrated with our recently developed image-processing program "HaematoCalMorph," the hyperspectral imaging system was useful for monitoring intracellular distributions of pigments during culture as well as for studying cellular responses under various conditions.
authors.affiliation: Laboratory of Signal Transduction, Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, FSB101, 5-1-5 Kashiwanoha, Kashiwa, Chiba, 277-8562, Japan.|JST-CREST, Kashiwa, 277-8562, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Nogami
authors.affiliation: Laboratory of Signal Transduction, Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, FSB101, 5-1-5 Kashiwanoha, Kashiwa, Chiba, 277-8562, Japan.|JST-CREST, Kashiwa, 277-8562, Japan.
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Ohnuki
authors.affiliation: Laboratory of Signal Transduction, Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, FSB101, 5-1-5 Kashiwanoha, Kashiwa, Chiba, 277-8562, Japan.|JST-CREST, Kashiwa, 277-8562, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Ohya
keywords: Haematococcus pluvialis; astaxanthin; digital image processing; hyperspectral imaging; morphology; nonnegative least square