title: Sunlight mediated synthesis of silver nanoparticles by a novel actinobacterium (Sinomonas mesophila MPKL 26) and its antimicrobial activity against multi drug resistant Staphylococcus aureus.
abstract: Synthesis of silver nanoparticles using microorganism are many, but there are only scanty reports using actinobacteria. In the present study, the actinobacterium of the genus Sinomonas was reported to synthesis silver nanoparticles for the first time. A photo-irradiation based method was developed for the synthesis of silver nanoparticles, which includes two day old cultural supernatant of novel species Sinomonas mesophila MPKL 26 and silver nitrate solution, exposed to sunlight. The preliminary synthesis of silver nanoparticles was noted by the color change of the solution from colorless to brown; the synthesis was further confirmed using UV-visible spectroscopy which shows a peak between 400 and 450nm. Spherical shape silver nanoparticles of size range 4-50nm were synthesized, which were characterized using transmission electron microscopy. The Fourier transform infrared spectroscopy result indicates that, the metabolite produced by the novel species S. mesophila MPKL 26 was the probable reducing/capping agent involved in the synthesis of silver nanoparticles. The synthesized silver nanoparticles maintained consistent shape with respect to different time periods. The synthesized silver nanoparticles were evaluated for the antimicrobial activity against multi drug resistant Staphylococcus aureus which show good antimicrobial activity. The method developed for synthesis is easy, requires less time (20min) and produces spherical shape nanoparticles of size as small as 4nm, having good antimicrobial activity. Hence, our study enlarges the scope of actinobacteria for the rapid biosynthesis of silver nanoparticles and can be used in formulating remedies for multi drug resistant S. aureus.
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China; Department of Microbiology, Gulbarga University, Gulbarga, Karnataka, India; Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China. Electronic address: deene.manik@gmail.com.
authors.forename: Deene
authors.initials: D
authors.lastname: Manikprabhu
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Cheng
authors.affiliation: China Tobacco Yunnan Industrial Co., Ltd, Kunming 650231, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Biochemistry, Gulbarga University, Gulbarga, Karnataka, India.
authors.forename: Anil Kumar
authors.initials: AK
authors.lastname: Sunkara
authors.affiliation: Department of Post-Graduate Studies and Research in Chemistry, Gulbarga University, Gulbarga 585 106, Karnataka, India.
authors.forename: Sunilkumar B
authors.initials: SB
authors.lastname: Mane
authors.affiliation: Department of Microbiology, Gulbarga University, Gulbarga, Karnataka, India.
authors.forename: Ram
authors.initials: R
authors.lastname: Kumar
authors.affiliation: Bioproducts Research Chair (BRC), College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
authors.forename: Mousumi
authors.initials: M
authors.lastname: das
authors.affiliation: Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef 62511, Egypt; Department of biotechnology, Siddaganga Institute of Technology, Tumkur, 572103, Karnataka, India.
authors.forename: Wael
authors.initials: W
authors.lastname: N Hozzein
authors.affiliation: China Tobacco Yunnan Industrial Co., Ltd, Kunming 650231, China.
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Duan
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China; Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China. Electronic address: liwenjun3@mail.sysu.edu.cn.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D053768:Metal Nanoparticles / Q000737:chemistry*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D008835:Micrococcaceae / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D012834:Silver / Q000737:chemistry*; D013472:Sunlight
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver
keywords: Antimicrobial activity; Silver nanoparticles; Sinomonas mesophila MPKL 26; Sunlight