title: A role for PDGF-C/PDGFRα signaling in the formation of the meningeal basement membranes surrounding the cerebral cortex.
abstract: Platelet-derived growth factor-C (PDGF-C) is one of three known ligands for the tyrosine kinase receptor PDGFRα. Analysis ofPdgfcnull mice has demonstrated roles for PDGF-C in palate closure and the formation of cerebral ventricles, but redundancy with other PDGFRα ligands might obscure additional functions. In search of further developmental roles for PDGF-C, we generated mice that were double mutants forPdgfc(-/-)andPdgfra(GFP/+) These mice display a range of severe phenotypes including spina bifida, lung emphysema, abnormal meninges and neuronal over-migration in the cerebral cortex. We focused our analysis on the central nervous system (CNS), where PDGF-C was identified as a critical factor for the formation of meninges and assembly of the glia limitans basement membrane. We also present expression data onPdgfa,PdgfcandPdgfrain the cerebral cortex and microarray data on cerebral meninges.
authors.affiliation: Department of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Uppsala 751 85, Sweden johanna.andrae@igp.uu.se.
authors.forename: Johanna
authors.initials: J
authors.lastname: Andrae
authors.affiliation: Department of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Uppsala 751 85, Sweden.
authors.forename: Leonor
authors.initials: L
authors.lastname: Gouveia
authors.affiliation: Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm 171 77, Sweden.
authors.forename: Radiosa
authors.initials: R
authors.lastname: Gallini
authors.affiliation: Department of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Uppsala 751 85, Sweden.
authors.forename: Liqun
authors.initials: L
authors.lastname: He
authors.affiliation: Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm 171 77, Sweden.
authors.forename: Linda
authors.initials: L
authors.lastname: Fredriksson
authors.affiliation: Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm 171 77, Sweden.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Nilsson
authors.affiliation: The Electron Microscopy Unit, Institute of Biomedicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg 405 30, Sweden.
authors.forename: Bengt R
authors.initials: BR
authors.lastname: Johansson
authors.affiliation: Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm 171 77, Sweden.
authors.forename: Ulf
authors.initials: U
authors.lastname: Eriksson
authors.affiliation: Department of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Uppsala 751 85, Sweden.
authors.forename: Christer
authors.initials: C
authors.lastname: Betsholtz
keywords: Basement membrane; Cerebrum; Meninges; PDGF-C; PDGFRα