title: Association of CD30 transcripts with Th1 responses and proinflammatory cytokines in patients with end-stage renal disease.
abstract: High serum sCD30 levels are associated with inflammatory disorders and poor outcome in renal transplantation. The contribution to these phenomena of transcripts and proteins related to CD30-activation and -cleavage is unknown. We assessed in peripheral blood of end-stage renal disease patients (ESRDP) transcripts of CD30-activation proteins CD30 and CD30L, CD30-cleavage proteins ADAM10 and ADAM17, and Th1- and Th2-type immunity-related factors t-bet and GATA3. Additionally, we evaluated the same transcripts and release of sCD30 and 32 cytokines after allogeneic and polyclonal T-cell activation. In peripheral blood, ESRDP showed increased levels of t-bet and GATA3 transcripts compared to healthy controls (HC) (both P<0.01) whereas levels of CD30, CD30L, ADAM10 and ADAM17 transcripts were similar. Polyclonal and allogeneic stimulation induced higher levels of CD30 transcripts in ESRDP than in HC (both P<0.001). Principal component analysis (PCA) in allogeneic cultures of ESRDP identified two correlation clusters, one consisting of sCD30, the Th-1 cytokine IFN-γ, MIP-1α, RANTES, sIL-2Rα, MIP-1β, TNF-β, MDC, GM-CSF and IL-5, and another one consisting of CD30 and t-bet transcripts, IL-13 and proinflammatory proteins IP-10, IL-8, IL-1Rα and MCP-1. Reflecting an activated immune state, ESRDP exhibited after allostimulation upregulation of CD30 transcripts in T cells, which was associated with Th1 and proinflammatory responses.
authors.affiliation: Grupo de Inmunología Celular e Inmunogenética, Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia.
authors.forename: Sonia Y
authors.initials: SY
authors.lastname: Velásquez
authors.affiliation: Department of Transplantation Immunology, Institute of Immunology, University of Heidelberg, Heidelberg, Germany.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Opelz
authors.affiliation: Grupo de Inmunología Celular e Inmunogenética, Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Rojas
authors.affiliation: Department of Transplantation Immunology, Institute of Immunology, University of Heidelberg, Heidelberg, Germany.
authors.forename: Caner
authors.initials: C
authors.lastname: Süsal
authors.affiliation: Grupo de Inmunología Celular e Inmunogenética, Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia. Electronic address: cristian.alvarez@udea.edu.co.
authors.forename: Cristiam M
authors.initials: CM
authors.lastname: Alvarez
mesh_terms: D000072197:ADAM10 Protein / Q000097:blood; D000072198:ADAM17 Protein / Q000097:blood; D000328:Adult; D053829:Amyloid Precursor Protein Secretases / Q000097:blood; D053285:CD30 Ligand / Q000097:blood* / Q000235:genetics; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D005260:Female; D050990:GATA3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D007519:Isoantigens / Q000276:immunology; D017730:Ki-1 Antigen / Q000097:blood* / Q000235:genetics; D007676:Kidney Failure, Chronic / Q000276:immunology*; D008297:Male; D008565:Membrane Proteins / Q000097:blood; D008875:Middle Aged; D020825:T-Box Domain Proteins / Q000235:genetics / Q000378:metabolism*; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D000098815:T-bet Transcription Factor
chemical_list: D053285:CD30 Ligand; D016207:Cytokines; D050990:GATA3 Transcription Factor; C494749:GATA3 protein, human; D018836:Inflammation Mediators; D007519:Isoantigens; D017730:Ki-1 Antigen; D008565:Membrane Proteins; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; C505774:TNFSF8 protein, human; D053829:Amyloid Precursor Protein Secretases; D000072197:ADAM10 Protein; C491236:ADAM10 protein, human; D000072198:ADAM17 Protein; C000606462:ADAM17 protein, human
keywords: Cytokines; End-stage renal disease patients; Gene expression; Mixed lymphocyte culture; sCD30