title: Circulating and visceral adipose miR-100 is down-regulated in patients with obesity and Type 2 diabetes.
abstract: Obesity is a major public health problem conferring substantial excess risk for Type 2 diabetes (T2D). The role of microRNAs (miRNAs) in obesity and adipose tissue is not clearly defined. We hypothesize that circulating miRNA expression profiles vary according to differences in body mass index (BMI) and T2D and circulating miRNAs may reflect adipose tissue expression. Compared to healthy, lean individuals, circulating miR-100 was significantly lower in obese normoglycemic subjects and subjects with T2D. In visceral adipose tissue, expression of miR-100 was lower from obese subjects with T2D compared to obese subjects without T2D. miR-100 expression was significantly lower after adipogenic induction in human visceral, subcutaneous adipocytes and 3T3-L1 adipocytes. miR-100 reduced expression of mammalian target of rapamycin (mTOR) and Insulin Growth Factor Receptor (IGFR) directly. Differentiation of 3T3-L1 was accelerated by inhibition of miR-100 and reduced by miR-100 mimic transfection. Our data provide the first evidence of an association of circulating miR-100 with obesity and diabetes. Additionally, our in-vitro findings, and the miR-100 expression patterns in site-specific adipose tissue suggest miR-100 to modulate IGFR, mTOR and mediate adipogenesis.
authors.affiliation: Clinical Research Unit, Khoo Teck Puat Hospital, 768828, Singapore. Electronic address: pek.sharon.lt@alexandrahealth.com.sg.
authors.forename: Sharon Li Ting
authors.initials: SL
authors.lastname: Pek
authors.affiliation: Diabetes Centre, Khoo Teck Puat Hospital, 768828, Singapore; Division of Endocrinology, Khoo Teck Puat Hospital, 768828, Singapore. Electronic address: sum.chee.fang@alexandrahealth.com.sg.
authors.forename: Chee Fang
authors.initials: CF
authors.lastname: Sum
authors.affiliation: Clinical Research Unit, Khoo Teck Puat Hospital, 768828, Singapore. Electronic address: lin.michelle.x@alexandrahealth.com.sg.
authors.forename: Michelle Xueqin
authors.initials: MX
authors.lastname: Lin
authors.affiliation: Department of General Surgery, Khoo Teck Puat Hospital, 768828, Singapore. Electronic address: cheng.anton.ks@alexandrahealth.com.sg.
authors.forename: Anton Kui Sing
authors.initials: AK
authors.lastname: Cheng
authors.affiliation: Health for Life, Khoo Teck Puat Hospital, 768828, Singapore. Electronic address: wong.michael.tk@alexandrahealth.com.sg.
authors.forename: Michael Tack Keong
authors.initials: MT
authors.lastname: Wong
authors.affiliation: Clinical Research Unit, Khoo Teck Puat Hospital, 768828, Singapore; Diabetes Centre, Khoo Teck Puat Hospital, 768828, Singapore; Division of Endocrinology, Khoo Teck Puat Hospital, 768828, Singapore. Electronic address: lim.su.chi@alexandrahealth.com.sg.
authors.forename: Su Chi
authors.initials: SC
authors.lastname: Lim
authors.affiliation: Clinical Research Unit, Khoo Teck Puat Hospital, 768828, Singapore; Diabetes Centre, Khoo Teck Puat Hospital, 768828, Singapore; Division of Endocrinology, Khoo Teck Puat Hospital, 768828, Singapore. Electronic address: subramaniam.tavintharan@alexandrahealth.com.sg.
authors.forename: Subramaniam
authors.initials: S
authors.lastname: Tavintharan
mesh_terms: D016475:3T3 Cells; D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D002478:Cells, Cultured; D015331:Cohort Studies; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000378:metabolism*; D015536:Down-Regulation; D005260:Female; D006801:Humans; D050152:Intra-Abdominal Fat / Q000378:metabolism*; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000097:blood / Q000378:metabolism*; D008875:Middle Aged; D009765:Obesity / Q000150:complications / Q000378:metabolism*; D017451:Receptors, Somatomedin / Q000378:metabolism; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism; D055815:Young Adult
chemical_list: C544266:MIRN100 microRNA, human; D035683:MicroRNAs; D017451:Receptors, Somatomedin; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases
keywords: Adipogenesis; IGFR; Obesity; Type 2 diabetes; mTOR; microRNAs