title: Effects of CC-chemokine receptor 5 on ROCK2 and P-MLC2 expression after focal cerebral ischaemia-reperfusion injury in rats.
abstract: CC-chemokine receptor 5 (CCR5) plays a pivotal role in reperfusion after stroke. This study assessed and confirmed the effects of CCR5 in experimental stroke via regulation of ROCK/P-MLC pathway.
  
  
  
  Male Sprague Dawley (SD) rats were randomly divided into sham group, ischaemia-reperfusion group (I/R group) and DAPTA group (I/R + CCR5 antagonist group). The rats of the I/R group were subjected to transient middle cerebral artery occlusion (tMCAO) for 2 hours, followed by 24 hours of reperfusion. Animals were measured for neurologic deficit, cerebral infarct volume, TUNEL and hematoxylin-eosin (HE) staining. The protein expressions of ROCK2 and P-MLC2(Ser19) were determined by western blot.
  
  
  
  Pre-treatment with DAPTA displayed significantly improved neurological functional outcome and reduced cerebral lesion compared with the I/R group animals (p < 0.05); HE staining showed that the I/R group had severe neuronal damage in the ischaemia core and penumbral; Compared with the I/R group, ROCK2 and P-MLC2(Ser19) protein expression in the DAPTA group was reduced (p < 0.05).
  
  
  
  The data demonstrate that CCR5 is correlated with up-regulation of the expression of ROCK2 and P-MLC2(Ser19) in the ischaemia cortex. Treated with CCR5 antagonist protects the brain against focal cerebral ischaemia-reperfusion injury in rats.
authors.affiliation: a Department of Neurology , Affiliated Hospital of Guilin Medical University.
authors.forename: Laisi
authors.initials: L
authors.lastname: Li
authors.affiliation: a Department of Neurology , Affiliated Hospital of Guilin Medical University.
authors.forename: Dongyi
authors.initials: D
authors.lastname: Zhi
authors.affiliation: b Department of Pathology.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Shen
authors.affiliation: a Department of Neurology , Affiliated Hospital of Guilin Medical University.
authors.forename: Kaixiang
authors.initials: K
authors.lastname: Liu
authors.affiliation: a Department of Neurology , Affiliated Hospital of Guilin Medical University.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.affiliation: c Department of Pathophysiology , Guilin Medical University , Guilin , PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D020520:Brain Infarction / Q000209:etiology; D024722:Cardiac Myosins / Q000378:metabolism*; D004195:Disease Models, Animal; D020287:In Situ Nick-End Labeling; D020244:Infarction, Middle Cerebral Artery / Q000378:metabolism* / Q000517:prevention & control; D008297:Male; D018994:Myosin Light Chains / Q000378:metabolism*; D009460:Neurologic Examination; D015717:Peptide T / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D019713:Receptors, CCR5 / Q000378:metabolism*; D015427:Reperfusion Injury / Q000378:metabolism*; D012694:Serine / Q000378:metabolism; D054460:rho-Associated Kinases / Q000378:metabolism*
chemical_list: D018994:Myosin Light Chains; D019713:Receptors, CCR5; C107184:myosin light chain 2; D015717:Peptide T; C055175:peptide T amide; D012694:Serine; C580818:ROCK2 protein, rat; D054460:rho-Associated Kinases; D024722:Cardiac Myosins
keywords: Cerebral ischemia–reperfusion injury; cc-chemokine receptor 5; myosin-regulatory light chain-2 phosphorylation; rho-kinase