title: Nano-patterned SU-8 surface using nanosphere-lithography for enhanced neuronal cell growth.
abstract: Mimicking the nanoscale surface texture of the extracellular matrix can affect the regulation of cellular behavior, including adhesion, differentiation, and neurite outgrowth. In this study, SU-8-based polymer surfaces with well-ordered nanowell arrays were fabricated using nanosphere lithography with polystyrene nanoparticles. We show that the SU-8 surface with nanowells resulted in similar neuronal development of rat pheochromocytoma (PC12) cells compared with an unpatterned poly-L-lysine (PLL)-coated SU-8 surface. Additionally, even after soaking the substrate in cell culture medium for two weeks, cells on the nanowell SU-8 surface showed long-term neurite outgrowth compared to cells on the PLL-coated SU-8 surface. The topographical surface modification of the nanowell array demonstrates potential as a replacement for cell adhesive material coatings such as PLL, for applications requiring long-term use of polymer-based implantable devices.
authors.affiliation: DGIST-ETH Microrobotics Research Center (DEMRC), Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 42988, Korea. Department of Robotics Engineering, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 42988, Korea.
authors.forename: Eunhee
authors.initials: E
authors.lastname: Kim
authors.forename: Seung-Jun
authors.initials: SJ
authors.lastname: Yoo
authors.forename: Eunjung
authors.initials: E
authors.lastname: Kim
authors.forename: Tae-Hwan
authors.initials: TH
authors.lastname: Kwon
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Cheil
authors.initials: C
authors.lastname: Moon
authors.forename: Hongsoo
authors.initials: H
authors.lastname: Choi
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004852:Epoxy Compounds / Q000737:chemistry* / Q000494:pharmacology; D054118:Nanospheres / Q000737:chemistry*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D016716:PC12 Cells; D010751:Phosphopyruvate Hydratase / Q000235:genetics / Q000378:metabolism; D011107:Polylysine / Q000737:chemistry; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D020917:Receptor, trkA / Q000235:genetics / Q000378:metabolism; D013499:Surface Properties
chemical_list: D015815:Cell Adhesion Molecules; D004852:Epoxy Compounds; D011108:Polymers; C520895:SU-8 compound; D011107:Polylysine; D020917:Receptor, trkA; D010751:Phosphopyruvate Hydratase