title: Decalcification using ethylenediaminetetraacetic acid for clear microstructure imaging of cochlea through optical coherence tomography.
abstract: The aim of this study was to analyze the effectiveness of decalcification using ethylenediaminetetraacetic acid (EDTA) as an optical clearing method to enhance the depth visibility of internal soft tissues of cochlea. Ex vivo mouse and guinea pig cochlea samples were soaked in EDTA solutions for decalcification, and swept source optical coherence tomography (OCT) was used as imaging modality to monitor the decalcified samples consecutively. The monitored noninvasive cross-sectional images showed that the mouse and guinea pig cochlea samples had to be decalcified for subsequent 7 and 14 days, respectively, to obtain the optimal optical clearing results. Using this method, difficulties in imaging of internal cochlea microstructures of mice could be evaded. The obtained results verified that the depth visibility of the decalcified ex vivo samples was enhanced.
authors.affiliation: Kyungpook National University, School of Electronics Engineering, College of IT Engineering, 80 Daehak-ro, Buk-gu, Daegu 41566, Republic of Korea.
authors.forename: Jaeyul
authors.initials: J
authors.lastname: Lee
authors.affiliation: Kyungpook National University, School of Electronics Engineering, College of IT Engineering, 80 Daehak-ro, Buk-gu, Daegu 41566, Republic of Korea.
authors.forename: Kanghae
authors.initials: K
authors.lastname: Kim
authors.affiliation: Kyungpook National University, School of Electronics Engineering, College of IT Engineering, 80 Daehak-ro, Buk-gu, Daegu 41566, Republic of Korea.
authors.forename: Ruchire Eranga
authors.initials: RE
authors.lastname: Wijesinghe
authors.affiliation: Kyungpook National University, School of Electronics Engineering, College of IT Engineering, 80 Daehak-ro, Buk-gu, Daegu 41566, Republic of Korea.
authors.forename: Doekmin
authors.initials: D
authors.lastname: Jeon
authors.affiliation: Daegu Veterans Hospital, Department of Otorhinolaryngology, 60 Wolgok-ro, Dalseo-gu, Daegu 42835, Republic of Korea.
authors.forename: Sang Heun
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Kyungpook National University, School of Electronics Engineering, College of IT Engineering, 80 Daehak-ro, Buk-gu, Daegu 41566, Republic of Korea.
authors.forename: Mansik
authors.initials: M
authors.lastname: Jeon
authors.affiliation: Ajou University, School of Medicine, Department of Otorhinolaryngology-Head and Neck Surgery, 164 World cup-ro, Yeongtong-gu, Suwon, Gyeonggi-do 41944, Republic of Korea.
authors.forename: Jeong Hun
authors.initials: JH
authors.lastname: Jang
mesh_terms: D000818:Animals; D003051:Cochlea / Q000737:chemistry* / Q000000981:diagnostic imaging*; D004492:Edetic Acid / Q000737:chemistry*; D006168:Guinea Pigs; D006652:Histological Techniques / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D041623:Tomography, Optical Coherence / Q000379:methods*
chemical_list: D004492:Edetic Acid