title: Impact of transduction towards the proliferation and migration as well as the transduction efficiency of human umbilical cord-derived late endothelial progenitor cells with nine recombinant adeno-associated virus serotypes.
abstract: OBJECTIVE
  To evaluate the transduction efficiency of human umbilical cord-derived, late endothelial progenitor cells late (HUCB-late EPCs) with nine recombinant adeno-associated virus (rAAV) serotypes and the ability of proliferation and migration of the cells after transduction.
  
  
  RESULTS
  rAAV2 and rAAV6 showed a greater ability than other serotypes to transduce late EPCs (P < 0.05). After transduction, cell proliferation ability weakened (P < 0.05), but the ability of migration to stromal cell-derived factor (SDF-1) unchanged.
  
  
  CONCLUSIONS
  There is an advantage of choosing the optimal rAAV serotype as a gene vector to alter the biologic characteristics of late EPCs.
authors.affiliation: Department of Cardiology of Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, 410000, China.
authors.forename: Zhaofei
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Cardiology of Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, 410000, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.affiliation: The Medical College of Hunan Normal University, Changsha, 410000, China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Cao
authors.affiliation: The Medical College of Hunan Normal University, Changsha, 410000, China.
authors.forename: Xiyun
authors.initials: X
authors.lastname: Deng
authors.affiliation: The Medical College of Hunan Normal University, Changsha, 410000, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.affiliation: The Medical College of Nanhua University, Hengyang, 421001, China.
authors.forename: Qiaofeng
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Cardiology of Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, 410000, China. yxdxwzf@163.com.
authors.forename: Zhaofen
authors.initials: Z
authors.lastname: Zheng
mesh_terms: D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation / Q000235:genetics / Q000502:physiology; D000229:Dependovirus / Q000235:genetics*; D066026:Endothelial Progenitor Cells / Q000166:cytology*; D006801:Humans; D014161:Transduction, Genetic / Q000379:methods*; D014470:Umbilical Cord / Q000166:cytology*
keywords: Human umbilical cord-derived late endothelial progenitor cells (HUCB-late EPCs); Recombinant adeno-associated virus (rAAV); Transduction efficiency