title: Simple, fast, and accurate methodology for quantitative analysis using Fourier transform infrared spectroscopy, with bio-hybrid fuel cell examples.
abstract: The standard methodologies for quantitative analysis (QA) of mixtures using Fourier transform infrared (FTIR) instruments have evolved until they are now more complicated than necessary for many users' purposes. We present a simpler methodology, suitable for widespread adoption of FTIR QA as a standard laboratory technique across disciplines by occasional users.•Algorithm is straightforward and intuitive, yet it is also fast, accurate, and robust.•Relies on component spectra, minimization of errors, and local adaptive mesh refinement.•Tested successfully on real mixtures of up to nine components. We show that our methodology is robust to challenging experimental conditions such as similar substances, component percentages differing by three orders of magnitude, and imperfect (noisy) spectra. As examples, we analyze biological, chemical, and physical aspects of bio-hybrid fuel cells.
authors.affiliation: U.S. Army Research Laboratory, 2800 Powder Mill Road, Adelphi, MD, USA.
authors.forename: David M
authors.initials: DM
authors.lastname: Mackie
authors.affiliation: U.S. Army Research Laboratory, 2800 Powder Mill Road, Adelphi, MD, USA.
authors.forename: Justin P
authors.initials: JP
authors.lastname: Jahnke
authors.affiliation: U.S. Army Research Laboratory, 2800 Powder Mill Road, Adelphi, MD, USA.
authors.forename: Marcus S
authors.initials: MS
authors.lastname: Benyamin
authors.affiliation: U.S. Army Research Laboratory, 2800 Powder Mill Road, Adelphi, MD, USA.
authors.forename: James J
authors.initials: JJ
authors.lastname: Sumner
keywords: FTIR; Fourier transform infrared; Fuel cell; Methodology; Quantitative analysis