title: Turn-off fluorescence sensor for the detection of ferric ion in water using green synthesized N-doped carbon dots and its bio-imaging.
abstract: This paper reports turn-off fluorescence sensor for Fe(3+) ion in water using fluorescent N-doped carbon dots as a probe. A simple and efficient hydrothermal carbonization of Prunus avium fruit extract for the synthesis of fluorescent nitrogen-doped carbon dots (N-CDs) is described. This green approach proceeds quickly and provides good quality N-CDs. The mean size of synthesized N-CDs was approximately 7nm calculated from the high-resolution transmission electron microscopic images. X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy revealed the presence of -OH, -NH2, -COOH, and -CO functional groups over the surface of CDs. The N-CDs showed excellent fluorescent properties, and emitted blue fluorescence at 411nm upon excitation at 310nm. The calculated quantum yield of the synthesized N-CDs is 13% against quinine sulfate as a reference fluorophore. The synthesized N-CDs were used as a fluorescent probe towards the selective and sensitive detection of biologically important Fe(3+) ions in water by fluorescence spectroscopy and for bio-imaging of MDA-MB-231 cells. The limit of detection (LOD) and the Stern-Volmer quenching constant for the synthesized N-CDs were 0.96μM and 2.0958×10(3)M of Fe(3+) ions. The green synthesized N-CDs are efficiently used as a promising candidate for the detection of Fe(3+) ions and bio-imaging.
authors.affiliation: School of Chemical Engineering, Yeungnam University, Gyeongsan 712-749, Republic of Korea.
authors.forename: Thomas Nesakumar Jebakumar Immanuel
authors.initials: TN
authors.lastname: Edison
authors.affiliation: School of Chemical Engineering, Yeungnam University, Gyeongsan 712-749, Republic of Korea.
authors.forename: Raji
authors.initials: R
authors.lastname: Atchudan
authors.affiliation: School of Chemical Engineering, Yeungnam University, Gyeongsan 712-749, Republic of Korea.
authors.forename: Jae-Jin
authors.initials: JJ
authors.lastname: Shim
authors.affiliation: Department of Nuclear Medicine, Kyungpook National University School of Medicine, Daegu 700-721, Republic of Korea.
authors.forename: Senthilkumar
authors.initials: S
authors.lastname: Kalimuthu
authors.affiliation: Department of Nuclear Medicine, Kyungpook National University School of Medicine, Daegu 700-721, Republic of Korea.
authors.forename: Byeong-Cheol
authors.initials: BC
authors.lastname: Ahn
authors.affiliation: School of Chemical Engineering, Yeungnam University, Gyeongsan 712-749, Republic of Korea. Electronic address: yrlee@yu.ac.kr.
authors.forename: Yong Rok
authors.initials: YR
authors.lastname: Lee
mesh_terms: D002244:Carbon / Q000737:chemistry*; D005290:Ferric Compounds / Q000032:analysis*; D005453:Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D014867:Water / Q000737:chemistry*
chemical_list: D005290:Ferric Compounds; D014867:Water; D002244:Carbon
keywords: Bio-imaging; Cytotoxicity; Fe(3+) sensor; Fluorescence; N-doped carbon dot; Prunus avium