title: Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering.
abstract: Thermoresponsive poly(N-isopropylacrylamide) (PIPAAm)-immobilized surfaces for controlling cell adhesion and detachment were fabricated by the Langmuir-Schaefer method. Amphiphilic block copolymers composed of polystyrene and PIPAAm (St-IPAAms) were synthesized by reversible addition-fragmentation chain transfer (RAFT) radical polymerization. A chloroform solution of St-IPAAm molecules was gently dropped into a Langmuir-trough apparatus, and both barriers of the apparatus were moved horizontally to compress the film to regulate its density. Then, the St-IPAAm Langmuir film was horizontally transferred onto a hydrophobically modified glass substrate by a surface-fixed device. Atomic force microscopy images clearly revealed nanoscale sea-island structures on the surface. The strength, rate, and quality of cell adhesion and detachment on the prepared surface were modulated by changes in temperature across the lower critical solution temperature range of PIPAAm molecules. In addition, a two-dimensional cell structure (cell sheet) was successfully recovered on the optimized surfaces. These unique PIPAAm surfaces may be useful for controlling the strength of cell adhesion and detachment.
authors.affiliation: Department of Physics, Tokyo University of Science.
authors.forename: Morito
authors.initials: M
authors.lastname: Sakuma
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University; kumashiro.yoshikazu@twmu.ac.jp.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Kumashiro
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University.
authors.forename: Masamichi
authors.initials: M
authors.lastname: Nakayama
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Tanaka
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Haraguchi
authors.affiliation: Department of Physics, Tokyo University of Science.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Umemura
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Shimizu
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamato
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University; tokano@twmu.ac.jp.
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
mesh_terms: D002448:Cell Adhesion / Q000502:physiology*; D057927:Hydrophobic and Hydrophilic Interactions; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D011108:Polymers