title: The Transcript Profile of a Traditional Chinese Medicine, Atractylodes lancea, Revealing Its Sesquiterpenoid Biosynthesis of the Major Active Components.
abstract: Atractylodes lancea (Thunb.) DC., named "Cangzhu" in China, which belongs to the Asteraceae family. In some countries of Southeast Asia (China, Thailand, Korea, Japan etc.) its rhizome, commonly called rhizoma atractylodis, is used to treat many diseases as it contains a variety of sesquiterpenoids and other components of medicinal importance. Despite its medicinal value, the information of the sesquiterpenoid biosynthesis is largely unknown. In this study, we investigated the transcriptome analysis of different tissues of non-model plant A. lancea by using short read sequencing technology (Illumina). We found 62,352 high quality unigenes with an average sequence length of 913 bp in the transcripts of A. Lancea. Among these, 43,049 (69.04%), 30,264 (48.53%), 26,233 (42.07%), 17,881 (28.67%) and 29,057(46.60%) unigenes showed significant similarity (E-value<1e(-5)) to known proteins in Nr, KEGG, SWISS-PROT, GO, and COG databases, respectively. Of the total 62,352 unigenes, 43,049 (Nr Database) open reading frames were predicted. On the basis of different bioinformatics tools we identify all the enzymes that take part in the terpenoid biosynthesis as well as five different known sesquiterpenoids via cytosolic mevalonic acid (MVA) pathway and plastidal methylerythritol phosphate (MEP) pathways. In our study, 6, 864 Simple Sequence Repeats (SSRs) were also found as great potential markers in A. lancea. This transcriptomic resource of A. lancea provides a great contribution in advancement of research for this specific medicinal plant and more specifically for the gene mining of different classes of terpenoids and other chemical compounds that have medicinal as well as economic importance.
authors.affiliation: Department of Medicinal Plant, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, P.R. China.|Center for Plant Functional Components, Huazhong Agricultural University, Wuhan, 430070, P.R. China.|National-Regional Joint Engineering Research Center in Hubei for Medicinal Plant Breeding and Cultivation, Huazhong Agricultural University, Wuhan, 430070, P.R. China.|Engineering Research Center for Medicinal Plants, Huazhong Agricultural University, Wuhan, 430070, P.R. China.
authors.forename: Shakeel
authors.initials: S
authors.lastname: Ahmed
authors.affiliation: Department of Medicinal Plant, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, P.R. China.|Center for Plant Functional Components, Huazhong Agricultural University, Wuhan, 430070, P.R. China.|National-Regional Joint Engineering Research Center in Hubei for Medicinal Plant Breeding and Cultivation, Huazhong Agricultural University, Wuhan, 430070, P.R. China.|Engineering Research Center for Medicinal Plants, Huazhong Agricultural University, Wuhan, 430070, P.R. China.
authors.forename: Chuansong
authors.initials: C
authors.lastname: Zhan
authors.affiliation: Department of Medicinal Plant, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, P.R. China.|National-Regional Joint Engineering Research Center in Hubei for Medicinal Plant Breeding and Cultivation, Huazhong Agricultural University, Wuhan, 430070, P.R. China.|Engineering Research Center for Medicinal Plants, Huazhong Agricultural University, Wuhan, 430070, P.R. China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Medicinal Plant, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, P.R. China.|National-Regional Joint Engineering Research Center in Hubei for Medicinal Plant Breeding and Cultivation, Huazhong Agricultural University, Wuhan, 430070, P.R. China.|Engineering Research Center for Medicinal Plants, Huazhong Agricultural University, Wuhan, 430070, P.R. China.
authors.forename: Xuekui
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Medicinal Plant, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, P.R. China.|National-Regional Joint Engineering Research Center in Hubei for Medicinal Plant Breeding and Cultivation, Huazhong Agricultural University, Wuhan, 430070, P.R. China.|Engineering Research Center for Medicinal Plants, Huazhong Agricultural University, Wuhan, 430070, P.R. China.
authors.forename: Tewu
authors.initials: T
authors.lastname: Yang
authors.affiliation: Department of Medicinal Plant, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, P.R. China.|National-Regional Joint Engineering Research Center in Hubei for Medicinal Plant Breeding and Cultivation, Huazhong Agricultural University, Wuhan, 430070, P.R. China.|Engineering Research Center for Medicinal Plants, Huazhong Agricultural University, Wuhan, 430070, P.R. China.
authors.forename: Zeying
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Medicinal Plant, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, P.R. China.|Center for Plant Functional Components, Huazhong Agricultural University, Wuhan, 430070, P.R. China.|National-Regional Joint Engineering Research Center in Hubei for Medicinal Plant Breeding and Cultivation, Huazhong Agricultural University, Wuhan, 430070, P.R. China.|Engineering Research Center for Medicinal Plants, Huazhong Agricultural University, Wuhan, 430070, P.R. China.
authors.forename: Qiyun
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Medicinal Plant, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, P.R. China.|Center for Plant Functional Components, Huazhong Agricultural University, Wuhan, 430070, P.R. China.|National-Regional Joint Engineering Research Center in Hubei for Medicinal Plant Breeding and Cultivation, Huazhong Agricultural University, Wuhan, 430070, P.R. China.|Engineering Research Center for Medicinal Plants, Huazhong Agricultural University, Wuhan, 430070, P.R. China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Medicinal Plant, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, P.R. China.|Center for Plant Functional Components, Huazhong Agricultural University, Wuhan, 430070, P.R. China.|National-Regional Joint Engineering Research Center in Hubei for Medicinal Plant Breeding and Cultivation, Huazhong Agricultural University, Wuhan, 430070, P.R. China.|Engineering Research Center for Medicinal Plants, Huazhong Agricultural University, Wuhan, 430070, P.R. China.
authors.forename: Xuebo
authors.initials: X
authors.lastname: Hu
mesh_terms: D031174:Atractylodes / Q000378:metabolism*; D053898:Biosynthetic Pathways; D019295:Computational Biology; D020869:Gene Expression Profiling; D008516:Medicine, Chinese Traditional; D018895:Microsatellite Repeats; D020641:Polymorphism, Single Nucleotide; D012333:RNA, Messenger / Q000378:metabolism; D012717:Sesquiterpenes / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D012717:Sesquiterpenes