title: Mechanism and In Vivo Evaluation: Photodynamic Antibacterial Chemotherapy of Lysine-Porphyrin Conjugate.
abstract: Lysine-porphyrin conjugate 4i has potent photosensitive antibacterial effect on clinical isolated bacterial strains such as Methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli, and Pseudomonas aeruginosa. The mechanism of photodynamic antibacterial chemotherapy of 4i (4i-PACT) in vitro and the treatment effect in vivo was investigated in this paper. Atomic force microscopy (AFM) revealed that 4i-PACT can effectively destroy membrane and wall of bacteria, resulting in leakage of its content. This was confirmed by dual fluorescent staining with acridine orange/ethidium bromide and measuring materials absorption at 260 nm. Agarose gel electrophoresis measurement showed that 4i-PACT can damage genomic DNA. Healing of wound in rat infected by mixed bacteria showed that the efficiency of 4i-PACT is dependent on the dose of light. These results showed that 4i-PACT has promising bactericidal effect both in vitro and in vivo.
authors.affiliation: Tianjin Key Laboratory of Biomedical Material, Institute of Biomedical Engineering, Peking Union Medical College - Chinese Academy of Medical Sciences Tianjin, China.
authors.forename: Zengping
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Materials Chemistry, Nankai University Tianjin, China.
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Tianjin Key Laboratory of Biomedical Material, Institute of Biomedical Engineering, Peking Union Medical College - Chinese Academy of Medical SciencesTianjin, China; Tianjin Medical University Cancer Institute and HospitalTianjin, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Meng
authors.affiliation: Tianjin Key Laboratory of Biomedical Material, Institute of Biomedical Engineering, Peking Union Medical College - Chinese Academy of Medical Sciences Tianjin, China.
authors.forename: Baochen
authors.initials: B
authors.lastname: Yang
authors.affiliation: Tianjin Key Laboratory of Biomedical Material, Institute of Biomedical Engineering, Peking Union Medical College - Chinese Academy of Medical Sciences Tianjin, China.
authors.forename: Liyun
authors.initials: L
authors.lastname: Pang
authors.affiliation: Tianjin Medical University Cancer Institute and Hospital Tianjin, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.affiliation: Tianjin Key Laboratory of Biomedical Material, Institute of Biomedical Engineering, Peking Union Medical College - Chinese Academy of Medical Sciences Tianjin, China.
authors.forename: Tianjun
authors.initials: T
authors.lastname: Liu
keywords: antibiotic resistance; lysine-porphyrin conjugate; mixed bacterial-infected wounds; photodynamic antimicrobial chemotherapy; photosensitizer