title: [Isolation of Actinomycete DF02 from Composting and Its Application in Biological Control of Botrytis cinerea].
abstract: OBJECTIVE
  To isolate and identify the biocontrol actinomycete from composting, and to investigate its potted control effect against Botrytis cinerea.
  
  
  METHODS
  Actinomycetes were isolated from composting by pour plate method. Inhibitory activities of acti- nomycetes were determined by dilution plate method and cylinder plate method. Strain with high activities was identified based on mor- phology and biochemical characterization and 16S rDNA gene sequence analysis. And its potted control effect was also investigated.
  
  
  RESULTS
  31 strains of actinomycetes were isolated, three of them had inhibitory effects on the test strains. Of which, the strain DF02 showed antagonistic to five plant pathogenic fungi of all the tested strains, and its inhibitory effect on Botrytis cinerea was the strongest. The results of potted control effect test showed that the protective and therapeutic efficacy of the fermentation suspension to Botrytis cinerea was 58.47% and 53.83%, respectively. According to taxonomic identification, the strain DF02 was identified as Strepto- myces neopeptinius.
  
  
  CONCLUSIONS
  Strain DF02 is a biocontrol actinomycete with broad development potential, and these results provide ex- perimental basis for the biocontrol of Botrytis cinerea.
authors.forename: Xue-jun
authors.initials: XJ
authors.lastname: Wang
authors.forename: Chang-li
authors.initials: CL
authors.lastname: Min
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
mesh_terms: D039903:Actinobacteria / Q000502:physiology*; D000898:Antibiosis; D061046:Biological Control Agents / Q000302:isolation & purification*; D020171:Botrytis / Q000472:pathogenicity*; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control; D012987:Soil; D012988:Soil Microbiology
chemical_list: D061046:Biological Control Agents; D012987:Soil