title: How to Build a Complex, Functional Propeller Protein, From Parts.
abstract: By combining ancestral sequence reconstruction and in vitro evolution, Smock et al. identified single motifs that assemble into a functional five-bladed β-propeller, and a likely route for conversion into the more complex, extant single chain fusion. Interestingly, although sequence diversification destabilized five-motif fusions, it also destabilized aggregation-prone intermediates, increasing the level of functional protein in vivo.
authors.affiliation: Department of Chemistry and Biochemistry, Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN 46556, USA. Electronic address: pclark1@nd.edu.
authors.forename: Patricia L
authors.initials: PL
authors.lastname: Clark
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D019143:Evolution, Molecular; D015870:Gene Expression; D006737:Horseshoe Crabs / Q000235:genetics / Q000378:metabolism; D037102:Lectins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D019151:Peptide Library; D000072757:Protein Conformation, beta-Strand; D015202:Protein Engineering / Q000379:methods*; D017510:Protein Folding; D054730:Protein Interaction Domains and Motifs; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012615:Sea Anemones / Q000235:genetics / Q000378:metabolism
chemical_list: D037102:Lectins; D019151:Peptide Library; D011994:Recombinant Proteins; C425304:Tachypleus plasma lectin-2
keywords: beta-propeller; protein fold; repeat protein structure; sequence evolution