title: Dexmedetomidine Protects against Transient Global Cerebral Ischemia/Reperfusion Induced Oxidative Stress and Inflammation in Diabetic Rats.
abstract: BACKGROUND
  Transient global cerebral ischemia/reperfusion (I/R) is a major perioperative complication, and diabetes increases the response of oxidative stress and inflammation induced by I/R. The objective of this study was to determine the protective effect of dexmedetomidine against transient global cerebral ischemia/reperfusion induced oxidative stress and inflammation in diabetic rats.
  
  
  METHODS
  Sixty-four rats were assigned into four experimental groups: normoglycemia, normoglycemia + dexmedetomidine, hyperglycemia, and hyperglycemia + dexmedetomidine and all subsequent neurological examinations were evaluated by a blinded observer. Damage to the brain was histologically assessed using the TUNEL staining method while western blotting was used to investigate changes in the expression levels of apoptosis-related proteins as well as the microglia marker, ionized calcium-binding adapter molecule 1 (Iba1). Water content in the brain was also analyzed. In addition, hippocampal concentrations of malondialdehyde (MDA) and Nox2 (a member of the Nox family of NADPH oxidases), and the activity of superoxide dismutase and catalase were analyzed. Finally, changes in serum concentrations of tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 were detected.
  
  
  RESULTS
  Results showed that diabetes increased brain water content, the number of apoptotic neurons, early neurological deficit scores, oxidative stress (MDA and Nox2) and inflammation (pro-inflammatory cytokines including TNF-α and IL-6) levels following transient global I/R injury, but that these symptoms were attenuated following administration of dexmedetomidine.
  
  
  CONCLUSIONS
  These findings suggest that dexmedetomidine can significantly alleviate damage resulting from I/R, and this mechanism may be related to a reduction in both oxidative stress and inflammation which is normally associated with I/R.
authors.affiliation: Department of Anaesthesiology, Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Xianzhang
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Department of Anaesthesiology, Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Honglei
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Anaesthesiology, Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Xichun
authors.initials: X
authors.lastname: Xing
authors.affiliation: Department of Anaesthesiology, Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Anaesthesiology, Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Li
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*; D016207:Cytokines / Q000097:blood; D020927:Dexmedetomidine / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D006439:Hemodynamics; D007249:Inflammation / Q000517:prevention & control*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*
chemical_list: D016207:Cytokines; D020927:Dexmedetomidine