title: Mycobacterium bovis-infected macrophages from resistant and susceptible cattle exhibited a differential pro-inflammatory gene expression profile depending on strain virulence.
abstract: Mycobacterium bovis, the causative agent of bovine tuberculosis, is an intracellular bacterium that normally persists inside host macrophages. However, the influence of bacterial virulence and host resistance on the final outcome in this interaction is not well known. In this study, we infected macrophages isolated from natural disease resistant (R) and susceptible (S) cattle donors with M. bovis strains characterized as attenuated and virulent to assess pro-inflammatory cytokine (TNFα, IL-12, IL-18, IL-1β, IL-6), chemokine (MCP-1, MCP-2, MIP-1), macrophage receptor (MSR1, TLR2, TLR4, MMR) and iNOS mRNA expression levels. Our findings identified a pro-inflammatory gene expression profile as a common feature after M. bovis infection regardless of bacterial virulence, however in S macrophages a superior expression was induced by the attenuated strain, whereas in R macrophages it was accomplished by the virulent M. bovis. A macrophage pro-inflammatory profile is intended to control M. bovis intracellular growth; however the host resistant phenotype plays a determinant role in it, since R macrophages had better intracellular bacterial control than S cells.
authors.affiliation: Laboratorio de Investigación en Tuberculosis Bovina, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Coyoacán, México, D.F. 04510, Mexico.
authors.forename: Edgar
authors.initials: E
authors.lastname: Alfonseca-Silva
authors.affiliation: Patología Experimental, Departamento de Patología, Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán", Vasco de Quiroga 15, Tlalpan, México, D.F. 14000, Mexico.
authors.forename: Rogelio
authors.initials: R
authors.lastname: Hernández-Pando
authors.affiliation: Laboratorio de Investigación en Tuberculosis Bovina, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Coyoacán, México, D.F. 04510, Mexico. Electronic address: jagp@unam.mx.
authors.forename: José A
authors.initials: JA
authors.lastname: Gutiérrez-Pabello
mesh_terms: D000818:Animals; D002417:Cattle / Q000276:immunology; D002478:Cells, Cultured; D018925:Chemokines / Q000235:genetics*; D016207:Cytokines / Q000235:genetics*; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology; D009163:Mycobacterium bovis / Q000472:pathogenicity*; D059467:Transcriptome; D014380:Tuberculosis, Bovine / Q000276:immunology*; D014774:Virulence
chemical_list: D018925:Chemokines; D016207:Cytokines
keywords: Bovine macrophages; Bovine tuberculosis; Mycobacterium bovis; Natural disease resistance