title: Immune responses induced by recombinant Bacillus subtilis expressing the spike protein of transmissible gastroenteritis virus in pigs.
abstract: Transmissible gastroenteritis (TGE) causes severe diarrhea in suckling piglets, results in enormous economic loss in swine-producing areas of the world. To develop an effective, safe, and convenient vaccine for the prevention of TGE, we have constructed a recombinant Bacillus subtilis strain (B. subtilis CotGSG) displaying the transmissible gastroenteritis virus (TGEV) spike (S) protein and discussed its immune function to intestinal submucosal dendritic cells (DCs). Our results showed that the recombinant B. subtilis had the ability to recruit more DCs to sample B. subtilis CotGSG, migrate to MLNs, and induce immune responses. Immunized piglets with B. subtilis CotGSG could significantly elevate the specific SIgA titers in feces, IgG titers and neutralizing antibodies in serum. Collectively, our results suggested that recombinant B. subtilis CotGSG expressing the TGEV S protein could effectively induce immune responses via DCs, and provided a perspective on potential novel strategy and approach that may be applicable to the development of the next generation of TGEV vaccines.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, 210095, PR China. Electronic address: ytqxmcx@163.com.
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Mou
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, 210095, PR China. Electronic address: zhuliqio2@163.com.
authors.forename: Liqi
authors.initials: L
authors.lastname: Zhu
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, 210095, PR China. Electronic address: 792672231@qq.com.
authors.forename: Xianping
authors.initials: X
authors.lastname: Xing
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, 210095, PR China. Electronic address: kinglj2004@163.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Lin
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, 210095, PR China. Electronic address: zxbyq@njau.edu.cn.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology; D001412:Bacillus subtilis / Q000235:genetics / Q000276:immunology*; D002465:Cell Movement; D003713:Dendritic Cells / Q000276:immunology; D005761:Gastroenteritis, Transmissible, of Swine / Q000276:immunology* / Q000517:prevention & control; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology / Q000821:virology; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology; D014764:Viral Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000956:Antigens, Viral; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines; C064484:spike protein S, Transmissible gastroenteritis virus
keywords: Bacillus subtilis; Dendritic cells; Immune responses; Oral immunization; Recombinant expression; TGEV S protein