title: Introduction of cationic virosome derived from vesicular stomatitis virus as a novel gene delivery system for sf9 cells.
abstract: Insect-derived cell lines are used extensively to produce recombinant proteins because they are capable of performing a range of post-translational modifications. Due to their significance in biotechnological applications, various methods have been developed to transfect them. In this study, we introduce a virosome constructed from vesicular stomatitis virus (VSV) as a new delivery system for sf9 cells. We labeled these VSV virosomes by fluorescent probe Rhodamine B chloride (R18). By fluorescence microscope observation and conducting a fusion assay, we confirmed the uptake of VSV virosomes via endocytosis by sf9 cells and their fusion with the endosomal membrane. Moreover, we incubated cationic VSV virosomes with a GFP-expressing bacmid and transfected sf9 cells, after 24 h some cells expressed GFP indicating the ability of VSV virosomes to deliver heterologous DNA to these cells. This is the first report of a virosome-based delivery system introduced for an insect cell line.
authors.affiliation: a Influenza Research Laboratory, Department of Virology and.
authors.forename: Yahya
authors.initials: Y
authors.lastname: Mohammadzadeh
authors.affiliation: a Influenza Research Laboratory, Department of Virology and.
authors.forename: Shima
authors.initials: S
authors.lastname: Gholami
authors.affiliation: a Influenza Research Laboratory, Department of Virology and.
authors.forename: Narges
authors.initials: N
authors.lastname: Rasouli
authors.affiliation: a Influenza Research Laboratory, Department of Virology and.
authors.forename: Sahar
authors.initials: S
authors.lastname: Sarrafzadeh
authors.affiliation: a Influenza Research Laboratory, Department of Virology and.
authors.forename: Nasim Sadat
authors.initials: NS
authors.lastname: Seyed Tabib
authors.affiliation: a Influenza Research Laboratory, Department of Virology and.
authors.forename: Mohammad Hasan
authors.initials: MH
authors.lastname: Samiee Aref
authors.affiliation: b Department of Hepatitis and AIDS , Pasteur Institute of Iran , Tehran , Iran.
authors.forename: Asghar
authors.initials: A
authors.lastname: Abdoli
authors.affiliation: a Influenza Research Laboratory, Department of Virology and.
authors.forename: Peyvand
authors.initials: P
authors.lastname: Biglari
authors.affiliation: a Influenza Research Laboratory, Department of Virology and.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Fotouhi
authors.affiliation: a Influenza Research Laboratory, Department of Virology and.
authors.forename: Behrokh
authors.initials: B
authors.lastname: Farahmand
authors.affiliation: a Influenza Research Laboratory, Department of Virology and.
authors.forename: Masoumeh
authors.initials: M
authors.lastname: Tavassoti Kheiri
authors.affiliation: a Influenza Research Laboratory, Department of Virology and.
authors.forename: Abbas
authors.initials: A
authors.lastname: Jamali
mesh_terms: D000818:Animals; D002412:Cations / Q000737:chemistry; D002478:Cells, Cultured; D018014:Gene Transfer Techniques; D061987:Sf9 Cells; D018411:Spodoptera; D014721:Vesicular stomatitis Indiana virus / Q000737:chemistry*; D022701:Virosomes / Q000737:chemistry
chemical_list: D002412:Cations; D022701:Virosomes
keywords: Bacmid; VSV-G; cationic virosome; delivery system; sf9; vesicular stomatitis virus