title: Preparation and characterization of aminoethyl hydroxypropyl methyl cellulose modified with nisin.
abstract: Nisin grafted aminoethyl hydroxypropyl methyl cellulose (AEHPMC) was prepared by an enzyme-catalyzed reaction in the presence of microbial transglutaminase (MTGase). AEHPMC was synthesized with 2-chloroethylamine hydrochloride (CEH) which was as an intermediate reactant. The parameters, which influenced the NH2% and the degree of substitution (DS), including reaction time, reaction temperature and the mass ratio of the reactants were investigated. Antioxidant activities of AEHPMC-nisin were evaluated by the scavenging activity of hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. With the DS and concentration increasing of AEHPMC-nisin, the radical scavenging activity increased. The Methylthiazol tetrazolium (MTT) assay indicated that AEHPMC-nisin had low-toxicity to L929 cells. Therefore, the derivative of HPMC may show a promising potential application in biomedical, food and pharmaceutical fields.
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan university of technology, Wuhan 430070, China. Electronic address: lihongfan2000@hotmail.com.
authors.forename: Lihong
authors.initials: L
authors.lastname: Fan
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan university of technology, Wuhan 430070, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Hu
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan university of technology, Wuhan 430070, China.
authors.forename: Zhihai
authors.initials: Z
authors.lastname: Hu
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan university of technology, Wuhan 430070, China.
authors.forename: Min
authors.initials: M
authors.lastname: Peng
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan university of technology, Wuhan 430070, China.
authors.forename: Huigao
authors.initials: H
authors.lastname: Wen
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan university of technology, Wuhan 430070, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan university of technology, Wuhan 430070, China.
authors.forename: Tan
authors.initials: T
authors.lastname: Wang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D002470:Cell Survival / Q000187:drug effects; D005021:Ethylamines / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D016166:Free Radical Scavengers / Q000494:pharmacology; D065347:Hypromellose Derivatives / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D008970:Molecular Weight; D009561:Nisin / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013997:Time Factors
chemical_list: D000975:Antioxidants; D005021:Ethylamines; D016166:Free Radical Scavengers; D009561:Nisin; D065347:Hypromellose Derivatives; C010076:2-chloroethylamine
keywords: Aminoethy hydroxypropyl methyl cellulose; Antioxidant activity; Nisin