title: Preparation of l-phenylalanine-imprinted solid-phase extraction sorbent by Pickering emulsion polymerization and the selective enrichment of l-phenylalanine from human urine.
abstract: A novel l-phenylalanine molecularly imprinted solid-phase extraction sorbent was synthesized by the combination of Pickering emulsion polymerization and ion-pair dummy template imprinting. Compared to other polymerization methods, the molecularly imprinted polymers thus prepared exhibit a high specific surface, large pore diameter, and appropriate particle size. The key parameters for solid-phase extraction were optimized, and the result indicated that the molecularly imprinted polymer thus prepared exhibits a good recovery of 98.9% for l-phenylalanine. Under the optimized conditions of the procedure, an analytical method for l-phenylalanine was well established. By comparing the performance of the molecularly imprinted polymer and a commercial reverse-phase silica gel, the obtained molecularly imprinted polymer as an solid-phase extraction sorbent is more suitable, exhibiting high precision (relative standard deviation 3.2%, n = 4) and a low limit of detection (60.0 ± 1.9 nmol·L(-1) ) for the isolation of l-phenylalanine. Based on these results, the combination of the Pickering emulsion polymerization and ion-pair dummy template imprinting is effective for preparing selective solid-phase extraction sorbents for the separation of amino acids and organic acids from complex biological samples.
authors.affiliation: Key Laboratory of Space Applied Physics and Chemistry, Ministry of Education, Department of Chemistry, School of Natural and Applied Science, Northwestern Polytechnical University, Xi'an, P. R. China.
authors.forename: Ji
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Space Applied Physics and Chemistry, Ministry of Education, Department of Chemistry, School of Natural and Applied Science, Northwestern Polytechnical University, Xi'an, P. R. China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Hu
authors.affiliation: Key Laboratory of Space Applied Physics and Chemistry, Ministry of Education, Department of Chemistry, School of Natural and Applied Science, Northwestern Polytechnical University, Xi'an, P. R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Guan
authors.affiliation: Key Laboratory of Space Applied Physics and Chemistry, Ministry of Education, Department of Chemistry, School of Natural and Applied Science, Northwestern Polytechnical University, Xi'an, P. R. China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Space Applied Physics and Chemistry, Ministry of Education, Department of Chemistry, School of Natural and Applied Science, Northwestern Polytechnical University, Xi'an, P. R. China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Qian
authors.affiliation: Key Laboratory of Space Applied Physics and Chemistry, Ministry of Education, Department of Chemistry, School of Natural and Applied Science, Northwestern Polytechnical University, Xi'an, P. R. China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Space Applied Physics and Chemistry, Ministry of Education, Department of Chemistry, School of Natural and Applied Science, Northwestern Polytechnical University, Xi'an, P. R. China.
authors.forename: Chunbao
authors.initials: C
authors.lastname: Du
authors.affiliation: Key Laboratory of Space Applied Physics and Chemistry, Ministry of Education, Department of Chemistry, School of Natural and Applied Science, Northwestern Polytechnical University, Xi'an, P. R. China.
authors.forename: Renyuan
authors.initials: R
authors.lastname: Song
mesh_terms: D000327:Adsorption; D004655:Emulsions / Q000737:chemistry; D006801:Humans; D054802:Molecular Imprinting; D010316:Particle Size; D010649:Phenylalanine / Q000652:urine*; D058105:Polymerization; D052616:Solid Phase Extraction; D013499:Surface Properties
chemical_list: D004655:Emulsions; D010649:Phenylalanine
keywords: Ionic liquid; L-Phenylalanine; Molecular imprinting; Pickering emulsion; Solid-phase extraction