title: Generation and Validation of Monoclonal Antibodies Against the Maltose Binding Protein.
abstract: The maltose binding protein (MBP) is a commonly used protein tag. Two monoclonal antibodies (mAbs) were generated against the MBP by immunizing mice with purified 6xHis-tagged MBP (6xHis-MBP). A nontoxic adjuvant cocktail of poly(I:C) and anti-CD40 mAb was used. The two mAbs, 3D7 and 2A1, are demonstrated to be effective in immunoprecipitation, immunoblotting, western blot hybridization, and the ELISA assay. These two mAbs are available individually or in combination at cost through the Developmental Studies Hybridoma Bank, a nonprofit National Resource created by the National Institutes of Health.
authors.affiliation: Developmental Studies Hybridoma Bank, The University of Iowa , Iowa City, Iowa.
authors.forename: Yang-Nim
authors.initials: YN
authors.lastname: Park
authors.affiliation: Developmental Studies Hybridoma Bank, The University of Iowa , Iowa City, Iowa.
authors.forename: Rebecca A
authors.initials: RA
authors.lastname: Glover
authors.affiliation: Developmental Studies Hybridoma Bank, The University of Iowa , Iowa City, Iowa.
authors.forename: Karla J
authors.initials: KJ
authors.lastname: Daniels
authors.affiliation: Developmental Studies Hybridoma Bank, The University of Iowa , Iowa City, Iowa.
authors.forename: David R
authors.initials: DR
authors.lastname: Soll
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D006825:Hybridomas; D015151:Immunoblotting; D058619:Maltose-Binding Proteins / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal; D058619:Maltose-Binding Proteins