title: [Effects of PINK1 gene on cell apoptosis and cell autophagy in neonatal mice with hypoxic-ischemic brain damage].
abstract: OBJECTIVE
  To study the effect of PINK1 (phosphatase and tensin homolog deleted on chromosome ten induced putative kinase 1) gene on cell apoptosis and cell autophagy in neonatal mice with hypoxic-ischemic brain damage (HIBD).
  
  
  METHODS
  Seventy-two wild-type C57BL/6 mice and 72 PINK1 gene knockout neonatal C57BL/6 mice were randomly divided into four groups: sham-operated wild-type (SWT), HIBD model wild-type (MWT), sham-operated knockout (SKO) and HIBD model knockout (MKO). HIBD model was prepared by low oxygen exposure for 2.5 hours after right carotid artery ligation. After 24 hours of hypoxia-ischemia treatment, TTC (2,3,5-triphenyl four azole nitrogen chloride) staining was used to measure brain infarct volume. The immunohistochemical staining was used to measure the expression of cell apoptosis protein cleaved-caspase-3 (CC3) in brain tissues. The TUNEL method was used to measure cell apoptosis. The immunofluorescence staining and Western blot were used to measure the expression of cell autophagy protein LC3.
  
  
  RESULTS
  Compared with the MWT group, the infarct volume of brain tissues was markedly reduced in the MKO group (P<0.05), the number of apoptotic cells and the cell apoptosis index were markedly decreased in the MKO group (P<0.05), the expression of apoptosis protein CC3 was significantly reduced in the MKO group (P<0.05), the expression of cell autophagy protein LC3 was significantly decreased in the MKO group, and the autophagy indicator LC3II/LC3I was also markedly reduced in the MKO group (P<0.05).
  
  
  CONCLUSIONS
  PINK1 gene knockout can protect neonatal mice from HIBD.
authors.affiliation: Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China. mudz@scu.edu.cn.
authors.forename: Yang
authors.initials: Y
authors.lastname: Huang
authors.forename: Hong-Ju
authors.initials: HJ
authors.lastname: Chen
authors.forename: Jiang-Hu
authors.initials: JH
authors.lastname: Zhu
authors.forename: Feng-Yan
authors.initials: FY
authors.lastname: Zhao
authors.forename: Yi
authors.initials: Y
authors.lastname: Qu
authors.forename: De-Zhi
authors.initials: DZ
authors.lastname: Mu
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D001343:Autophagy; D005260:Female; D020925:Hypoxia-Ischemia, Brain / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011494:Protein Kinases / Q000235:genetics*; D012097:Repressor Proteins / Q000032:analysis; D025521:Tumor Suppressor Proteins / Q000032:analysis; D000099202:PTEN-Induced Putative Kinase
chemical_list: D011494:Protein Kinases; D012097:Repressor Proteins; D025521:Tumor Suppressor Proteins; D000099202:PTEN-Induced Putative Kinase; C525546:Tip30 protein, mouse