title: Optical fiber sensor based on surface plasmon resonance for rapid detection of avian influenza virus subtype H6: Initial studies.
abstract: A side-polished fiber optic surface plasmon resonance (SPR) sensor was fabricated to expose the core surface and then deposited with a 40 nm thin gold film for the near surface sensing of effective refractive index changes with surface concentration or thickness of captured avian influenza virus subtype H6. The detection surface of the SPR optical fiber sensor was prepared through the plasma modification method for binding a self-assembled monolayer of isopropanol chemically on the gold surface of the optical fiber. Subsequently, N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide/N-hydroxysuccinimide was activated to enable EB2-B3 monoclonal antibodies to capture A/chicken/Taiwan/2838V/00 (H6N1) through a flow injection system. The detection limit of the fabricated optical fiber sensor for A/chicken/Taiwan/2838V/00 was 5.14 × 10(5) EID50/0.1 mL, and the response time was 10 min on average. Moreover, the fiber optic sensor has the advantages of a compact size and low cost, thus rendering it suitable for online and remote sensing. The results indicated that the optical fiber sensor can be used for epidemiological surveillance and diagnosing of avian influenza subtype H6 rapidly.
authors.affiliation: Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Laboratory of Novel Reactor and Green Chemical Technology, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan 430073, China; Institute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Xihong
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Graduate Program in Electro-Optical Engineering, Tatung University, Chongshan North Road, Third Section, Taipei 104, Taiwan.
authors.forename: Yu-Chia
authors.initials: YC
authors.lastname: Tsao
authors.affiliation: Institute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Fu-Jung
authors.initials: FJ
authors.lastname: Lee
authors.affiliation: Graduate Program in Electro-Optical Engineering, Tatung University, Chongshan North Road, Third Section, Taipei 104, Taiwan.
authors.forename: Woo-Hu
authors.initials: WH
authors.lastname: Tsai
authors.affiliation: School of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan.
authors.forename: Ching-Ho
authors.initials: CH
authors.lastname: Wang
authors.affiliation: Institute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Tsung-Liang
authors.initials: TL
authors.lastname: Chuang
authors.affiliation: Graduate Program in Electro-Optical Engineering, Tatung University, Chongshan North Road, Third Section, Taipei 104, Taiwan.
authors.forename: Mu-Shiang
authors.initials: MS
authors.lastname: Wu
authors.affiliation: Institute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan; Institute of Biomedical Electronic and Bioinformatics, National Taiwan University, Taipei 10617, Taiwan; Center for Emerging Material and Advanced Devices, National Taiwan University, Taipei 10617, Taiwan. Electronic address: cwlinx@ntu.edu.tw.
authors.forename: Chii-Wann
authors.initials: CW
authors.lastname: Lin
mesh_terms: D000818:Animals; D000956:Antigens, Viral; D015374:Biosensing Techniques; D001717:Birds; D004797:Enzyme-Linked Immunosorbent Assay; D009980:Influenza A virus / Q000145:classification* / Q000235:genetics / Q000276:immunology; D005585:Influenza in Birds / Q000175:diagnosis / Q000821:virology; D055100:Optical Fibers; D020133:Reverse Transcriptase Polymerase Chain Reaction; D065288:Serogroup; D020349:Surface Plasmon Resonance
chemical_list: D000956:Antigens, Viral
keywords: Avian influenza virus subtype H6; Optical fiber; Rapid detection; Surface plasmon resonance