title: Modulation of IL-12 and IFNγ by probiotic supplementation promotes protection against Toxocara canis infection in mice.
abstract: In this study, supplementation with the probiotic Saccharomyces boulardii promoted a reduction in intensity of infection by Toxocara canis and modulates cytokines mRNA expression in experimentally infected mice. IL-12 gene transcription had 40-fold increase in S. boulardii supplemented uninfected mice and sevenfold increase in supplemented infected mice comparing with not supplemented group. Regarding IFNγ, similar results were observed, since probiotic supplementation induced approximately 43-fold increase, but only in uninfected mice (P < 0·05). T. canis infection upregulated IL-10 expression while S. boulardii downregulated it and no change was observed for IL-4. Thus, based in these findings; we suggest that one possible mechanism responsible for S. boulardii protection effect against T. canis infection is by the modulation of cytokines expression, especially IL-12.
authors.affiliation: Post-Graduate Program in Parasitology, Universidade Federal de Pelotas (UFPel), Pelotas, Brazil.
authors.forename: L F D C
authors.initials: LF
authors.lastname: de Avila
authors.affiliation: Center for Technological Development - Biotechnology, UFPel, Pelotas, Brazil.
authors.forename: P M M
authors.initials: PM
authors.lastname: de Leon
authors.affiliation: Post-Graduate Program in Parasitology, Universidade Federal de Pelotas (UFPel), Pelotas, Brazil.
authors.forename: M Q
authors.initials: MQ
authors.lastname: de Moura
authors.affiliation: Post-Graduate Program in Parasitology, Universidade Federal de Pelotas (UFPel), Pelotas, Brazil.
authors.forename: M E A
authors.initials: ME
authors.lastname: Berne
authors.affiliation: Post-Graduate Program in Health Science, Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul, Brazil.
authors.forename: C J
authors.initials: CJ
authors.lastname: Scaini
authors.affiliation: Post-Graduate Program in Parasitology, Universidade Federal de Pelotas (UFPel), Pelotas, Brazil.|Center for Technological Development - Biotechnology, UFPel, Pelotas, Brazil.
authors.forename: F P
authors.initials: FP
authors.lastname: Leivas Leite
mesh_terms: D000818:Animals; D016207:Cytokines / Q000276:immunology; D005260:Female; D007371:Interferon-gamma / Q000276:immunology*; D018664:Interleukin-12 / Q000276:immunology*; D051379:Mice; D019936:Probiotics / Q000008:administration & dosage*; D000069983:Saccharomyces boulardii; D017166:Toxocara canis / Q000502:physiology*; D014120:Toxocariasis / Q000276:immunology* / Q000517:prevention & control*
chemical_list: D016207:Cytokines; D018664:Interleukin-12; D007371:Interferon-gamma
keywords: Saccharomyces boulardii; immunomodulatory; innate immunity; toxocariasis