title: Kinetic Profile of Inflammation Markers in Human Skin In vivo Following Exposure to Ultraviolet B Indicates Synchronic Release of Cytokines and Prostanoids.
abstract: Ultraviolet B (UVB) irradiation affects epidermal cells, which respond via a cascade of inflammation markers. After initial in vitro and ex vivo experiments, this study used cutaneous microdialysis to generate a kinetic profile for 16 cytokines and 4 prostanoids in human skin in vivo. Skin areas 9 cm2 were irradiated with UVB (2× minimal erythematous dose) 16 h after catheter placement in the dermis of the volar forearms of healthy volunteers. Dialysates were collected at 4-h intervals up to 64 h and analysed for 5- and 8-iso-PGF2α, 9α,11α-PGF2α and PGE2 by gas chromatography-mass spectrometry (GC/MS). Dialysates were also analysed for interleukin (IL)-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-8, IL-10, tumour necrosis factor (TNF)-α, Fas ligand (FasL), interferon-γ-inducible protein-10 (IP-10), monocyte chemoattractant protein 1 (MCP-1), RANTES, eotaxin, and granulocyte-macrophage colony-stimulating factor (GM-CSF) using a multiplex-based cytometric-bead-array. In conclusion, 3 peaks with synchronic release of T helper (TH) 1-directed inflammatory cytokines and prostanoids could be detected post-UVB: an early phase (4-12 h), an intermediate phase (16-24 h) and a late phase (32-40 h). A TH2-directed cytokine response was detectable at intermediate and late phases.
authors.affiliation: Department of Dermatology and Venereology, Otto-von-Guericke University, Leipziger Str. 44, DE-39120 Magdeburg, Germany. squist@gmx.de.
authors.forename: Sven R
authors.initials: SR
authors.lastname: Quist
authors.forename: Ingrid
authors.initials: I
authors.lastname: Wiswedel
authors.forename: Jennifer
authors.initials: J
authors.lastname: Quist
authors.forename: Harald P
authors.initials: HP
authors.lastname: Gollnick
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D005542:Forearm; D008401:Gas Chromatography-Mass Spectrometry; D064368:Healthy Volunteers; D006801:Humans; D008297:Male; D017551:Microdialysis; D008875:Middle Aged; D011453:Prostaglandins / Q000378:metabolism*; D012867:Skin / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D016207:Cytokines; D011453:Prostaglandins