title: The transcription factor VpCRZ1 is required for fruiting body formation and pathogenicity in Valsa pyri.
abstract: Valsa pyri is a fatal pathogenic fungus that causes pear and apple canker disease. To date, its cellular development and pathogenicity have been poorly understood. In this study, a V. pyri Ca(2+)/calcineurin-dependent transcription factor CRZ1 (VpCRZ1) is identified and functionally characterized. The △VpCRZ1 mutant exhibits impaired pathogenicity and is no longer able to form fruiting body. Interestingly, this mutant also exhibits enhanced pigment deposition and increased resistance to cell wall perturbing agents including SDS, Congo red and calcofluor white (CFW). The expression levels of Congo red resistance genes (VpRCR1 and VpRCR2) and chitin synthetase genes (VpCHS2 and VpCHS6) are upregulated in the △VpCRZ1 mutant compared to the wild type. Furthermore, We show that a VpCRZ1: eGFP fusion protein localizes to the nucleus in a Ca(2+)-dependent manner similar to its homologs in other fungi, and that the VpFKS1, VpPMC1, VpPMC2, VpPMR1, and VpPMA1 genes are regulated by VpCRZ1 in response to Ca(2+) levels. Together, these results suggest that VpCRZ1 is a Ca(2+)-dependent transcription factor and required for regulating mycelial morphology, fruiting body formation, and virulence of this important pear and apple pathogen.
authors.affiliation: Department of Plant Pathology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Feng
authors.initials: F
authors.lastname: He
authors.affiliation: Department of Plant Pathology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Plant Pathology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Joseph Juma
authors.initials: JJ
authors.lastname: Mafurah
authors.affiliation: Department of Plant Pathology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Meixiang
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Plant Pathology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Qian
authors.affiliation: Department of Plant Pathology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Rongbo
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Plant Pathology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Asma
authors.initials: A
authors.lastname: Safdar
authors.affiliation: Department of Plant Pathology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Plant Pathology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Fengquan
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Plant Pathology, Nanjing Agricultural University, Nanjing, Jiangsu, China. Electronic address: ddou@njau.edu.cn.
authors.forename: Daolong
authors.initials: D
authors.lastname: Dou
mesh_terms: D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000494:pharmacology; D001203:Ascomycota / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity*; D001557:Benzenesulfonates / Q000494:pharmacology; D019703:Calcineurin / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D003223:Congo; D003224:Congo Red / Q000494:pharmacology; D048690:Fruiting Bodies, Fungal / Q000235:genetics / Q000254:growth & development*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D027845:Malus; D008853:Microscopy; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology; D031989:Pyrus; D017386:Sequence Homology, Amino Acid; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014774:Virulence
chemical_list: D000935:Antifungal Agents; D001557:Benzenesulfonates; D010860:Pigments, Biological; D014157:Transcription Factors; D012967:Sodium Dodecyl Sulfate; D003224:Congo Red; C007061:C.I. Fluorescent Brightening Agent 28; D019703:Calcineurin; D002118:Calcium
keywords: Calcineurin; Cell wall stress; Valsa pyri; Virulence; VpCRZ1