title: Desmethylanhydroicaritin isolated from Sophora flavescens, shows antitumor activities in U87MG cells via inhibiting the proliferation, migration and invasion.
abstract: This study is the first report of the antitumor activities of desmethylanhydroicaritin (DMAI) isolated from Sophora flavescens on U87MG cells. Human glioblastoma is one of the most aggressive malignant type of brain tumors and highly diffuses to around normal brain tissues. DMAI showed anti-proliferation effects on U87MG cells at the concentration of 30μM, however did not affect to HEK-293 cells. DMAI induced anti-proliferation effects via ERK/MAPK, PI3K/Akt/mTOR signal pathway and G2/M phase cell cycle arrest. DMAI led to morphological change and inhibition of filapodia formation through regulation of Rac 1 and Cdc 42. In addition, migration and invasion of U87MG cells were inhibited by DMAI via down-regulation of matrix metalloproteinase (MMP) -2 and MMP -9 expressions and activities. Our results suggest that DMAI has a potential as a therapeutic agent against glioblastoma cells.
authors.affiliation: Department of Microbiology, College of Natural Science, Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: Chang-Won
authors.initials: CW
authors.lastname: Kang
authors.affiliation: Department of Microbiology, College of Natural Science, Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: Nan-Hee
authors.initials: NH
authors.lastname: Kim
authors.affiliation: Department of Food Science and Human Nutrition, Chonbuk National University, Jeonju 54896, Republic of Korea.
authors.forename: Huyn Ah
authors.initials: HA
authors.lastname: Jung
authors.affiliation: Department of Microbiology, College of Natural Science, Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: Hyung-Wook
authors.initials: HW
authors.lastname: Choi
authors.affiliation: Department of Microbiology, College of Natural Science, Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: Min-Jae
authors.initials: MJ
authors.lastname: Kang
authors.affiliation: Department of Food and Life Science, College of Fisheries Science, Pukyong National University, Busan 48513, Republic of Korea. Electronic address: choijs@pknu.ac.kr.
authors.forename: Jae-Sue
authors.initials: JS
authors.lastname: Choi
authors.affiliation: Department of Microbiology, College of Natural Science, Pukyong National University, Busan 48513, Republic of Korea. Electronic address: gundokim@pknu.ac.kr.
authors.forename: Gun-Do
authors.initials: GD
authors.lastname: Kim
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D047309:Flavones / Q000494:pharmacology*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9; D009361:Neoplasm Invasiveness; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D029909:Sophora; D058570:TOR Serine-Threonine Kinases
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D047309:Flavones; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases; C539396:desmethylanhydroicaritin; C546842:MTOR protein, human
keywords: Desmethylanhydroicaritin; Invasion; Migration; Mitogen-activated protein kinase; Proliferation; U87MG