title: Quantification of Hydrazine in Human Urine by HPLC-MS-MS.
abstract: Currently used on F-16 fighter jets and some space shuttles, hydrazine could be released at toxic levels to humans as a result of an accidental leakage or spill. Lower-level exposures occur in industrial workers or as a result of the use of some pharmaceuticals. A method was developed for the quantitation of hydrazine in human urine and can be extended by dilution with water to cover at least six orders of magnitude, allowing measurement at all clinically significant levels of potential exposure. Urine samples were processed by isotope dilution, filtered, derivatized and then quantified by HPLC-MS-MS. The analytical response ratio was linearly proportional to the urine concentration of hydrazine from 0.0493 to 12.3 ng/mL, with an average correlation coefficientRof 0.9985. Inter-run accuracy for 21 runs, expressed as percent relative error (% RE), was ≤14%, and the corresponding precision, expressed as percent relative standard deviation (% RSD), was ≤15%. Because this method can provide a quantitative measurement of clinical samples over six orders of magnitude, it can be used to monitor trace amounts of hydrazine exposure as well as industrial and environmental exposure levels.
authors.affiliation: Battelle Memorial Institute at the Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Samantha L
authors.initials: SL
authors.lastname: Isenberg
authors.affiliation: Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA vsm8@cdc.gov.
authors.forename: Melissa D
authors.initials: MD
authors.lastname: Carter
authors.affiliation: Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA.
authors.forename: Brian S
authors.initials: BS
authors.lastname: Crow
authors.affiliation: Battelle Memorial Institute at the Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Leigh Ann
authors.initials: LA
authors.lastname: Graham
authors.affiliation: Oak Ridge Institute for Science and Education Fellow at the Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Darryl
authors.initials: D
authors.lastname: Johnson
authors.affiliation: New Mexico Department of Health, Scientific Lab Division, Chemical Threat Analytical Response Section, Albuquerque, NM, Mexico.
authors.forename: Nick
authors.initials: N
authors.lastname: Beninato
authors.affiliation: Oak Ridge Institute for Science and Education Fellow at the Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Kandace
authors.initials: K
authors.lastname: Steele
authors.affiliation: Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA.
authors.forename: Jerry D
authors.initials: JD
authors.lastname: Thomas
authors.affiliation: Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA.
authors.forename: Rudolph C
authors.initials: RC
authors.lastname: Johnson
mesh_terms: D002138:Calibration; D002273:Carcinogens / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004781:Environmental Exposure; D006801:Humans; D006834:Hydrazines / Q000652:urine*; D011786:Quality Control; D011865:Radioisotope Dilution Technique; D015203:Reproducibility of Results; D012996:Solutions; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D002273:Carcinogens; D006834:Hydrazines; D012996:Solutions; C029424:hydrazine