title: Mangiferin corrects the imbalance of Th17/Treg cells in mice with TNBS-induced colitis.
abstract: In the previous study, 80% ethanol extract of the rhizome mixture of Anemarrhena asphodeloides and Coptidis chinensis (AC) and its main constituent mangiferin improved TNBS-induced colitis in mice by inhibiting macrophage activation related to the innate immunity. In the preliminary study, we found that AC could inhibit Th17 cell differentiation in mice with TNBS-induced colitis. Therefore, we investigated whether AC and it main constituent mangiferin are capable of inhibiting inflammation by regulating T cell differentiation related to the adaptive immunity in vitro and in vivo. AC and mangiferin potently suppressed colon shortening and myeloperoxidase activity in mice with TNBS-induced colitis. They also suppressed TNBS-induced Th17 cell differentiation and IL-17 expression, but increased TNBS-suppressed Treg cell differentiation and IL-10 expression. Moreover, AC and mangiferin strongly inhibited the expression of TNF-α and IL-17, as well as the activation of NF-κB. Furthermore, mangiferin potently inhibited the differentiation of splenocytes into Th7 cells and increased the differentiation into Treg cells in vitro. Mangiferin also inhibited RORγt and IL-17 expression and STAT3 activation in splenocytes and induced Foxp3 and IL-10 expression and STAT5 activation. Based on these findings, mangiferin may ameliorate colitis by the restoration of disturbed Th17/Treg cells and inhibition of macrophage activation.
authors.affiliation: Department of Life and Nanopharmaceutical Sciences and Department of Pharmacy, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Korea.
authors.forename: Su-Min
authors.initials: SM
authors.lastname: Lim
authors.affiliation: Department of Life and Nanopharmaceutical Sciences and Department of Pharmacy, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Korea.
authors.forename: Jin-Ju
authors.initials: JJ
authors.lastname: Jeong
authors.affiliation: DongWha Pharm Research Institute, 35-71, Topsil-ro, Giheung-gu, Yongin-Shi, Gyeonggi 46-902, Republic of Korea.
authors.forename: Hyun Sik
authors.initials: HS
authors.lastname: Choi
authors.affiliation: DongWha Pharm Research Institute, 35-71, Topsil-ro, Giheung-gu, Yongin-Shi, Gyeonggi 46-902, Republic of Korea.
authors.forename: Hwan Bong
authors.initials: HB
authors.lastname: Chang
authors.affiliation: Department of Life and Nanopharmaceutical Sciences and Department of Pharmacy, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Korea. Electronic address: dhkim@khu.ac.kr.
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Kim
mesh_terms: D031403:Anemarrhena / Q000276:immunology; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology; D000089042:Coptis chinensis; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006706:Homeostasis; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism; D020381:Interleukin-17 / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3 / Q000235:genetics / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects* / Q000276:immunology; D058504:Th17 Cells / Q000187:drug effects* / Q000276:immunology; D014302:Trinitrobenzenesulfonic Acid; D044004:Xanthones / Q000627:therapeutic use*
chemical_list: D004365:Drugs, Chinese Herbal; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D020381:Interleukin-17; D016328:NF-kappa B; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; D044004:Xanthones; D016753:Interleukin-10; C013592:mangiferin; D014302:Trinitrobenzenesulfonic Acid; C107393:Coptidis rhizoma extract
keywords: Colitis; Macrophage; Mangiferin; Th17 cell; Treg cell