title: MicroRNA Regulatory Mechanisms on Citrus sinensis leaves to Magnesium-Deficiency.
abstract: Magnesium (Mg)-deficiency, which affects crop productivity and quality, widespreadly exists in many agricultural crops, including citrus. However, very limited data are available on Mg-deficiency-responsive microRNAs (miRNAs) in higher plants. Using Illumina sequencing, we isolated 75 (73 known and 2 novel) up- and 71 (64 known and 7 novel) down-regulated miRNAs from Mg-deficient Citrus sinensis leaves. In addition to the remarkable metabolic flexibility as indicated by the great alteration of miRNA expression, the adaptive responses of leaf miRNAs to Mg-deficiency might also involve the following several aspects: (a) up-regulating stress-related genes by down-regulating miR164, miR7812, miR5742, miR3946, and miR5158; (b) enhancing cell transport due to decreased expression of miR3946 and miR5158 and increased expression of miR395, miR1077, miR1160, and miR8019; (c) activating lipid metabolism-related genes by repressing miR158, miR5256, and miR3946; (d) inducing cell wall-related gene expansin 8A by repressing miR779; and (e) down-regulating the expression of genes involved in the maintenance of S, K and Cu by up-regulating miR395 and miR6426. To conclude, we isolated some new known miRNAs (i.e., miR7812, miR8019, miR6218, miR1533, miR6426, miR5256, miR5742, miR5561, miR5158, and miR5818) responsive to nutrient deficiencies and found some candidate miRNAs that might contribute to Mg-deficiency tolerance. Therefore, our results not only provide novel information about the responses of plant to Mg-deficiency, but also are useful for obtaining the key miRNAs for plant Mg-deficiency tolerance.
authors.affiliation: Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Life Science, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Horticulture, Fujian Agriculture and Forestry UniversityFuzhou, China.
authors.forename: Cui-Lan
authors.initials: CL
authors.lastname: Ma
authors.affiliation: Institute of Materia Medica, Fujian Academy of Medical Sciences Fuzhou, China.
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Qi
authors.affiliation: Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Resource and Environmental Science, Fujian Agriculture and Forestry UniversityFuzhou, China.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Liang
authors.affiliation: Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Resource and Environmental Science, Fujian Agriculture and Forestry UniversityFuzhou, China.
authors.forename: Lin-Tong
authors.initials: LT
authors.lastname: Yang
authors.affiliation: Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Resource and Environmental Science, Fujian Agriculture and Forestry UniversityFuzhou, China.
authors.forename: Yi-Bin
authors.initials: YB
authors.lastname: Lu
authors.affiliation: Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Resource and Environmental Science, Fujian Agriculture and Forestry UniversityFuzhou, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Guo
authors.affiliation: Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Resource and Environmental Science, Fujian Agriculture and Forestry UniversityFuzhou, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Ye
authors.affiliation: Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Resource and Environmental Science, Fujian Agriculture and Forestry UniversityFuzhou, China; The Higher Educational Key Laboratory of Fujian Province for Soil Ecosystem Health and Regulation, Fujian Agriculture and Forestry UniversityFuzhou, China; Fujian Key Laboratory for Plant Molecular and Cell Biology, Fujian Agriculture and Forestry UniversityFuzhou, China.
authors.forename: Li-Song
authors.initials: LS
authors.lastname: Chen
keywords: Citrus sinensis; Illumina sequencing; Mg-deficiency; leaves; microRNA