title: Mutagenic and chemical analyses provide new insight into enzyme activation and mechanism of the type 2 iron-sulfur l-serine dehydratase from Legionella pneumophila.
abstract: The crystal structure of the Type 2 l-serine dehydratase from Legionella pneumophila (lpLSD), revealed a "tail-in-mouth" configuration where the C-terminal residue acts as an intrinsic competitive inhibitor. This pre-catalytic structure undergoes an activation step prior to catalytic turnover. Mutagenic analysis of residues at or near the active site cleft is consistent with stabilization of substrate binding by many of the same residues that interact with the C-terminal cysteine and highlight the critical role of certain tail residues in activity. pH-rate profiles show that a residue with pK of 5.9 must be deprotonated and a residue with a pK of 8.5 must be protonated for activity. This supports an earlier suggestion that His 61 is the likely catalytic base. An additional residue with a pK of 8.5-9 increases cooperativity when it is deprotonated. This investigation also demonstrates that the Fe-S dehydratases convert the enamine/imine intermediates of the catalytic reaction to products on the enzyme prior to release. This is in contrast to pyridoxyl 5' phosphate based dehydratases that release an enamine/imine intermediate into solution, which then hydrolyzes to produce the ketoamine product.
authors.affiliation: Developmental Biology, Washington University School of Medicine, 660 S. Euclid Avenue, Box 8103, St. Louis, MO 63110, USA.
authors.forename: Xiao Lan
authors.initials: XL
authors.lastname: Xu
authors.affiliation: Developmental Biology, Washington University School of Medicine, 660 S. Euclid Avenue, Box 8103, St. Louis, MO 63110, USA; Department of Medicine, Washington University School of Medicine, 660 S. Euclid Avenue, Box 8103, St. Louis, MO 63110, USA. Electronic address: ggrant@wustl.edu.
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Grant
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D002384:Catalysis; D004789:Enzyme Activation / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D012695:L-Serine Dehydratase / Q000737:chemistry* / Q000235:genetics; D016952:Legionella pneumophila / Q000201:enzymology* / Q000235:genetics; D016296:Mutagenesis
chemical_list: D001426:Bacterial Proteins; D012695:L-Serine Dehydratase
keywords: Dehydratase; Iron-sulfur; l-Serine