title: Disaccharide analysis of chondroitin and heparin from farmed Atlantic salmon.
abstract: The heparin disaccharides detected in farmed Atlantic salmon (Salmo salar) gills and intestines have, with one exception, been reported in porcine heparin. The relative amounts of disaccharides appear to be very different in the two species. Two chondroitin disaccharides with a proposed essential role in the zebrafish (Danio rerio) development and differentiation are detected in farmed Atlantic salmon. In addition, most of the chondroitin/dermatan sulfate and heparin disaccharides detected here have been reported in zebrafish, in support of the claims of the heparin presence in fish. The same chondroitin/dermatan disaccharides were detected in the bones of bony fishes. The rare disaccharide UA2S-GalNAc that was found in trace amounts in all 5 bony fishes was found in relative high amounts in gills and in significant amounts in intestines. The rare heparin disaccharide UA2S-GlcN was in relative highest amounts both in gills and intestines. In context with our previous reports, this communication suggests that glycosaminoglycans in farmed Atlantic salmon heparin need further studies in order to clarify structure and function.
authors.affiliation: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), N-1432, Ås, Norway. ragnar.flengsrud@nmbu.no.
authors.forename: Ragnar
authors.identifier: http://orcid.org/0000-0002-6384-8696
authors.initials: R
authors.lastname: Flengsrud
mesh_terms: D000818:Animals; D002807:Chondroitin / Q000737:chemistry / Q000302:isolation & purification; D004187:Disaccharides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D006493:Heparin / Q000737:chemistry / Q000302:isolation & purification; D020317:Salmo salar; D013329:Structure-Activity Relationship; D015027:Zebrafish
chemical_list: D004187:Disaccharides; D006493:Heparin; D002807:Chondroitin
keywords: Atlantic salmon; Chondroitin disaccharides; Heparin disaccharides; Heparin pentasaccharide