title: White adipose tissue IFN-γ expression and signalling along the progression of rodent cancer cachexia.
abstract: Cachexia is associated with increased morbidity and mortality in cancer. The White adipose tissue (WAT) synthesizes and releases several pro-inflammatory cytokines that play a role in cancer cachexia-related systemic inflammation. IFN-γ is a pleiotropic cytokine that regulates several immune and metabolic functions. To assess whether IFN-γ signalling in different WAT pads is modified along cancer-cachexia progression, we evaluated IFN-γ receptors expression (IFNGR1 and IFNGR2) and IFN-γ protein expression in a rodent model of cachexia (7, 10, and 14days after tumour implantation). IFN-γ protein expression was heterogeneously modulated in WAT, with increases in the mesenteric pad and decreased levels in the retroperitoneal depot along cachexia progression. Ifngr1 was up-regulated 7days after tumour cell injection in mesenteric and epididymal WAT, but the retroperitoneal depot showed reduced Ifngr1 gene expression. Ifngr2 gene expression was increased 7 and 14days after tumour inoculation in mesenteric WAT. The results provide evidence that changes in IFN-γ expression and signalling may be perceived at stages preceding refractory cachexia, and therefore, might be employed as a means to assess the early stage of the syndrome.
authors.affiliation: Department of Physiology and Biophysics, Institute of Biomedical Sciences and Faculdade de Medicina, University of Sao Paulo, São Paulo, Brazil.
authors.forename: Alex Shimura
authors.initials: AS
authors.lastname: Yamashita
authors.affiliation: Cancer Metabolism Research Group, Institute of Biomedical Sciences and Faculdade de Medicina, University of Sao Paulo, São Paulo, Brazil.
authors.forename: Rodrigo Xavier
authors.initials: RX
authors.lastname: das Neves
authors.affiliation: Cancer Metabolism Research Group, Institute of Biomedical Sciences and Faculdade de Medicina, University of Sao Paulo, São Paulo, Brazil.
authors.forename: José Cesar
authors.initials: JC
authors.lastname: Rosa-Neto
authors.affiliation: Immunometabolism Research Group, Department of Physical Education, Universidade Estadual Paulista (UNESP), Presidente Prudente, SP, Brazil.
authors.forename: Fábio Dos Santos
authors.initials: FD
authors.lastname: Lira
authors.affiliation: Laboratory of Adipose Tissue Biology, Center for Integrated Biotechnology, University of Mogi das Cruzes, Mogi das Cruzes, São Paulo, Brazil.
authors.forename: Miguel Luís
authors.initials: ML
authors.lastname: Batista
authors.affiliation: Cancer Metabolism Research Group, Institute of Biomedical Sciences and Faculdade de Medicina, University of Sao Paulo, São Paulo, Brazil; Department of Clinical Surgery, University Hospital, University of Sao Paulo, São Paulo, Brazil.
authors.forename: Paulo Sérgio
authors.initials: PS
authors.lastname: Alcantara
authors.affiliation: Cancer Metabolism Research Group, Institute of Biomedical Sciences and Faculdade de Medicina, University of Sao Paulo, São Paulo, Brazil; Department of Clinical Surgery, University Hospital, University of Sao Paulo, São Paulo, Brazil.
authors.forename: José Pinhata
authors.initials: JP
authors.lastname: Otoch
authors.affiliation: Cancer Metabolism Research Group, Institute of Biomedical Sciences and Faculdade de Medicina, University of Sao Paulo, São Paulo, Brazil. Electronic address: seelaend@icb.usp.br.
authors.forename: Marília
authors.initials: M
authors.lastname: Seelaender
mesh_terms: D052436:Adipose Tissue, White / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D002100:Cachexia / Q000378:metabolism* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D007371:Interferon-gamma / Q000096:biosynthesis*; D008297:Male; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D009374:Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D017471:Receptors, Interferon / Q000096:biosynthesis; D015398:Signal Transduction; D000096743:Interferon gamma Receptor
chemical_list: D007371:Interferon-gamma; D009363:Neoplasm Proteins; D017471:Receptors, Interferon; D000096743:Interferon gamma Receptor
keywords: Cancer-cachexia; Interferon-γ signalling; White adipose tissue