title: Ethanol-induced conditioned taste aversion in Warsaw Alcohol High-Preferring (WHP) and Warsaw Alcohol Low-Preferring (WLP) rats.
abstract: The aversive action of the pharmacological properties of ethanol was studied in selectively bred Warsaw Alcohol High-Preferring (WHP) and Warsaw Alcohol Low-Preferring (WLP) rats. For this study, a conditioned-taste aversion test was used. Male WHP and WLP rats were submitted to daily 20-min sessions for 5 days, in which a saccharin solution (1.0 g/L) was available (pre-conditioning phase). Next, this drinking was paired with the injection of ethanol (0, 0.5, 1.0 g/kg), intraperitoneally [i.p.] immediately after removal of the saccharin bottle (conditioning phase). Afterward, the choice between the saccharin solution and water was extended for 18 subsequent days for 20-min daily sessions (post-conditioning phase). Both doses of ethanol did not produce an aversion to saccharin in WLP and WHP rats in the conditioning phase. However, injection of the 1.0 g/kg dose of ethanol produced an aversion in WLP rats that was detected by a decrease in saccharin intake at days 1, 3, 7, and 10 of the post-conditioning phase, with a decrease in saccharin preference for 16 days of the post-conditioning phase. Conditioned taste aversion, measured as a decrease in saccharin intake and saccharin preference, was only visible in WHP rats at day 1 and day 3 of the post-conditioning phase. This difference between WLP and WHP rats was apparent despite similar blood ethanol levels in both rat lines following injection of 0.5 and 1.0 g/kg of ethanol. These results may suggest differing levels of aversion to the post-ingestional effects of ethanol between WLP and WHP rats. These differing levels of aversion may contribute to the selected line difference in ethanol preference in WHP and WLP rats.
authors.affiliation: Department of Pharmacology, Institute of Psychiatry and Neurology, Sobieskiego St. 9, 02-957 Warsaw, Poland. Electronic address: wdyr@ipin.edu.pl.
authors.forename: Wanda
authors.initials: W
authors.lastname: Dyr
authors.affiliation: Department of Pharmacology, Institute of Psychiatry and Neurology, Sobieskiego St. 9, 02-957 Warsaw, Poland.
authors.forename: Edyta
authors.initials: E
authors.lastname: Wyszogrodzka
authors.affiliation: Department of Pharmacology, Institute of Psychiatry and Neurology, Sobieskiego St. 9, 02-957 Warsaw, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Paterak
authors.affiliation: Department of Forensic Medicine, Warsaw Medical University, Oczki St. 1, 02-007 Warsaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Siwińska-Ziółkowska
authors.affiliation: Department of Pharmacology, Warsaw Medical University, Banacha St. 1, 02-097 Warsaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Małkowska
authors.affiliation: Toxicological Laboratory of Hospital Prague, Solidarności St. 67, 03-401 Warsaw, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Polak
mesh_terms: D000428:Alcohol Drinking / Q000235:genetics*; D000437:Alcoholism / Q000235:genetics; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects / Q000502:physiology*; D002755:Choice Behavior / Q000187:drug effects / Q000502:physiology*; D003214:Conditioning, Classical / Q000187:drug effects / Q000502:physiology*; D000431:Ethanol / Q000008:administration & dosage*; D008297:Male; D051381:Rats; D012439:Saccharin / Q000008:administration & dosage; D013649:Taste / Q000235:genetics*
chemical_list: D000431:Ethanol; D012439:Saccharin
keywords: Conditioned taste aversion (CTA) paradigm; Ethanol aversive properties; Warsaw Alcohol High-Preferring (WHP) rats; Warsaw Alcohol Low-Preferring (WLP) rats