title: The in vitro effect of cypermethrin on quality and oxidative stress indices of rainbow trout Oncorhynchus mykiss spermatozoa.
abstract: There is limited information on the scientific literature about the effect of in vitro exposure of fish sperm to pesticides. In vitro effect of cypermethrin on sperm quality and oxidative stress has not yet been fully investigated. Therefore, the effects of cypermethrin, a type II pyrethroid insecticide, on quality and oxidative stress of spermatozoa were examined in vitro. To explore the potential in vitro toxicity of cypermethrin, fish spermatozoa were incubated with different concentrations of cypermethrin (1.025, 2.05 and 4.1 μg/l) for 2 h. The motility rate and duration of sperm were determined after exposure to cypermethrin. Reduced glutathione (GSH), glutathione peroxidase (GSH-Px), catalase (CAT) and malondialdehyde (MDA) in spermatozoa were analyzed for determination of oxidant and antioxidant balance. Our results indicated that spermatozoa motility and duration significantly decreased with exposure to cypermethrin. Additionally, activity of GSH-Px (P<0.05) and MDA and GSH levels increased in a concentration-dependent manner while CAT activity decreased (P<0.05). Consequently, the oxidant and antioxidant status and sperm quality were affected by quantitative changes and different concentrations of cypermethrin.
authors.affiliation: Tunceli University, Fisheries Faculty, 62000, Tunceli, Turkey. Electronic address: filizkutluyer@hotmail.com.
authors.forename: Filiz
authors.initials: F
authors.lastname: Kutluyer
authors.affiliation: Tunceli University, Faculty of Engineering, Department of Food Engineering, 62000, Tunceli, Turkey.
authors.forename: Fulya
authors.initials: F
authors.lastname: Benzer
authors.affiliation: Fırat University, Faculty of Veterinary, Department of Basic Sciences, Elazığ, Turkey.
authors.forename: Mine
authors.initials: M
authors.lastname: Erişir
authors.affiliation: Muğla Sıtkı Koçman University, Faculty of Science, Department of Bology, 48000, Muğla, Turkey.
authors.forename: Fatih
authors.initials: F
authors.lastname: Öğretmen
authors.affiliation: Muğla Sıtkı Koçman University, Faculty of Science, Department of Bology, 48000, Muğla, Turkey.
authors.forename: Burak Evren
authors.initials: BE
authors.lastname: İnanan
mesh_terms: D000818:Animals; D066298:In Vitro Techniques; D007306:Insecticides / Q000633:toxicity*; D008297:Male; D017686:Oncorhynchus mykiss; D018384:Oxidative Stress / Q000187:drug effects*; D011722:Pyrethrins / Q000633:toxicity*; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism
chemical_list: D007306:Insecticides; D011722:Pyrethrins; C017160:cypermethrin
keywords: Cypermethrin; Oncorhynchus mykiss; Oxidative stress indices; Rainbow trout; Sperm quality; Spermatozoa