title: The role of Alix in the proliferation of human glioma cells.
abstract: Apoptosis-linked-gene-2-interacting protein 1 (Alix) is involved in the endosome-lysosome system in the cytoplasm. The normal function of Alix may be altered by ALG-2 toward a destructive role during active cell death. Alix also may play a role in regulation of cell proliferation. However, the role of Alix in human glioma has not been elucidated yet. This study intended to clarify the relationship between Alix and glioma pathologic grades and its role in the proliferation of glioma cells. Our findings showed that Alix protein concentrations were significantly elevated in high-grade glioma tissue compared with low-grade glioma (P < .0001). Immunohistochemical study revealed that Alix was overexpressed in 75 resected glioma tissues and may forecast poor survival. Alix expression was increased in resting serum-stimulated glioma cells. Additionally, we reduced Alix expression in U251MG cells and then found that cell viability was decreased significantly when p21 expression increased. Colony formation assay and flow cytometry analysis demonstrated that reduced Alix expression may lead to growth inhibition and cell cycle arrest. In summary, our findings suggest that Alix plays an important role in the proliferation of glioma cells and may be a novel therapeutic target.
authors.affiliation: Department of Neurosurgery, Affiliated Second Peoples Hospital of NanTong, Nantong University, Nantong, 226002, Jiangsu Province, People's Republic of China.
authors.forename: Chengjin
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Department of Pathology, Medical College and Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226001.
authors.forename: Na
authors.initials: N
authors.lastname: Ban
authors.affiliation: Department of Neurosurgery, Affiliated Second Peoples Hospital of NanTong, Nantong University, Nantong, 226002, Jiangsu Province, People's Republic of China.
authors.forename: Shirong
authors.initials: S
authors.lastname: Dai
authors.affiliation: Department of Neurosurgery, Affiliated Second Peoples Hospital of NanTong, Nantong University, Nantong, 226002, Jiangsu Province, People's Republic of China.
authors.forename: Xiubing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Medical College and Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226001.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Medical College and Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226001.
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226001.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Chen
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226001.
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.affiliation: The First Affiliated Hospital of Soochow University, Suzhou, 215006, Jiangsu Province.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Bao
authors.affiliation: Department of Neurosurgery, Wuxi Second People's Hospital of Nanjing Medical University, Wuxi, 214002, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Chang
authors.affiliation: Department of Pathology, Medical College and Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226001.
authors.forename: Donglin
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Affiliated Second Peoples Hospital of NanTong, Nantong University, Nantong, 226002, Jiangsu Province, People's Republic of China. Electronic address: jianbingren288@sina.com.
authors.forename: Jianbing
authors.initials: J
authors.lastname: Ren
mesh_terms: D000328:Adult; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D056827:Endosomal Sorting Complexes Required for Transport / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D034622:RNA Interference; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection
chemical_list: D002135:Calcium-Binding Proteins; D018797:Cell Cycle Proteins; D056827:Endosomal Sorting Complexes Required for Transport; C117020:PDCD6IP protein, human
keywords: Alix; Glioma; Prognosis; Proliferation; Survival