title: Maternal and postweaning folic acid supplementation interact to influence body weight, insulin resistance, and food intake regulatory gene expression in rat offspring in a sex-specific manner.
abstract: Maternal intake of multivitamins or folic acid above the basal dietary requirement alters the growth and metabolic trajectory of rat offspring. We hypothesized that a modest increase in the folic acid content of maternal diets would alter the offspring's metabolic phenotype, and that these effects could be corrected by matching the folic acid content of the offspring's diet with that of the maternal diet. Female Sprague-Dawley rats were placed on a control or a 2.5× folic acid-supplemented diet prior to mating and during pregnancy and lactation. At weaning, pups from each maternal diet group were randomized to the control or to the 2.5× folic acid-supplemented diet for 25 weeks. Male pups from dams fed the folic acid-supplemented diet were 3.7% heavier than those from control-fed dams and had lower mRNA expression for leptin receptor Obrb isoform (Lepr) (11%) and Agouti-related protein (Agrp) (14%). In contrast, female pups from folic acid-supplemented dams were 5% lighter than those from control-fed dams and had lower proopiomelanocortin (Pomc) (42%), Lepr (32%), and Agrp (13%), but higher neuropeptide Y (Npy) (18%) mRNA expression. Folic acid supplementation ameliorated the alterations induced by maternal folic acid supplementation in male pups and led to the lowest insulin resistance, but the effects were smaller in female pups and led to the highest insulin resistance. In conclusion, maternal folic acid supplementation at 2.5× the control level was associated with alterations in body weight and hypothalamic gene expression in rat offspring in a sex-specific manner, and some of these effects were attenuated by postweaning folic acid supplementation.
authors.affiliation: a Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, ON M5S 3E2, Canada.
authors.forename: Pedro S P
authors.initials: PS
authors.lastname: Huot
authors.affiliation: a Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, ON M5S 3E2, Canada.
authors.forename: Anna
authors.initials: A
authors.lastname: Ly
authors.affiliation: a Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, ON M5S 3E2, Canada.
authors.forename: Ignatius M Y
authors.initials: IM
authors.lastname: Szeto
authors.affiliation: a Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, ON M5S 3E2, Canada.
authors.forename: Sandra A
authors.initials: SA
authors.lastname: Reza-López
authors.affiliation: a Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, ON M5S 3E2, Canada.
authors.forename: Daniel
authors.initials: D
authors.lastname: Cho
authors.affiliation: a Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, ON M5S 3E2, Canada.|b Department of Medicine, Faculty of Medicine, University of Toronto, Toronto, ON M5G 2C4, Canada.|c Keenan Research Centre for Biomedical Sciences of St. Michael's Hospital, Toronto, ON M5B 1W8, Canada.|d Division of Gastroenterology, St. Michael's Hospital, Toronto, ON M5B 1W8, Canada.
authors.forename: Young-In
authors.initials: YI
authors.lastname: Kim
authors.affiliation: a Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, ON M5S 3E2, Canada.
authors.forename: G Harvey
authors.initials: GH
authors.lastname: Anderson
mesh_terms: D054369:Agouti-Related Protein / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001835:Body Weight / Q000187:drug effects*; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D005260:Female; D005492:Folic Acid / Q000008:administration & dosage* / Q000097:blood; D005786:Gene Expression Regulation; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D039382:Maternal Nutritional Physiological Phenomena; D009751:Nutritional Requirements; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000097:blood / Q000188:drug therapy; D011333:Pro-Opiomelanocortin / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D054411:Receptors, Leptin / Q000235:genetics / Q000378:metabolism; D012737:Sex Factors; D014886:Weaning
chemical_list: C515197:AGRP protein, rat; D054369:Agouti-Related Protein; D007328:Insulin; D012333:RNA, Messenger; D054411:Receptors, Leptin; D011333:Pro-Opiomelanocortin; D005492:Folic Acid
keywords: apport alimentaire; body weight; folate/acide folique; folate–folic acid; food intake; insulin resistance; insulinorésistance; masse corporelle; maternal supplementation; supplémentation de la mère