title: H2 -Fueled ATP Synthesis on an Electrode: Mimicking Cellular Respiration.
abstract: ATP, the molecule used by living organisms to supply energy to many different metabolic processes, is synthesized mostly by the ATPase synthase using a proton or sodium gradient generated across a lipid membrane. We present evidence that a modified electrode surface integrating a NiFeSe hydrogenase and a F1 F0 -ATPase in a lipid membrane can couple the electrochemical oxidation of H2 to the synthesis of ATP. This electrode-assisted conversion of H2 gas into ATP could serve to generate this biochemical fuel locally when required in biomedical devices or enzymatic synthesis of valuable products.
authors.affiliation: Instituto de Catálisis y Petroleoquímica, CSIC, c/ Marie Curie 2, 28049, Madrid, Spain.
authors.forename: Óscar
authors.initials: Ó
authors.lastname: Gutiérrez-Sanz
authors.affiliation: Universidad Complutense de Madrid, Avda. Complutense s/n, 28040, Madrid, Spain.|Instituto de Investigación i+12 hospital 12 de Octubre, Avda. Córdoba s/n, 28041, Madrid, Spain.
authors.forename: Paolo
authors.initials: P
authors.lastname: Natale
authors.affiliation: Instituto de Catálisis y Petroleoquímica, CSIC, c/ Marie Curie 2, 28049, Madrid, Spain.
authors.forename: Ileana
authors.initials: I
authors.lastname: Márquez
authors.affiliation: Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Apartado 127, 2781-901, Oeiras, Portugal.
authors.forename: Marta C
authors.initials: MC
authors.lastname: Marques
authors.affiliation: Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Apartado 127, 2781-901, Oeiras, Portugal.
authors.forename: Sonia
authors.initials: S
authors.lastname: Zacarias
authors.affiliation: Instituto de Catálisis y Petroleoquímica, CSIC, c/ Marie Curie 2, 28049, Madrid, Spain.
authors.forename: Marcos
authors.initials: M
authors.lastname: Pita
authors.affiliation: Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Apartado 127, 2781-901, Oeiras, Portugal.
authors.forename: Inês A C
authors.initials: IA
authors.lastname: Pereira
authors.affiliation: Universidad Complutense de Madrid, Avda. Complutense s/n, 28040, Madrid, Spain. ivanlopez@quim.ucm.es.|Instituto de Investigación i+12 hospital 12 de Octubre, Avda. Córdoba s/n, 28041, Madrid, Spain. ivanlopez@quim.ucm.es.
authors.forename: Iván
authors.initials: I
authors.lastname: López-Montero
authors.affiliation: Instituto de Catálisis y Petroleoquímica, CSIC, c/ Marie Curie 2, 28049, Madrid, Spain. alopez@icp.csic.es.
authors.forename: Antonio L
authors.initials: AL
authors.lastname: De Lacey
authors.affiliation: Instituto de Catálisis y Petroleoquímica, CSIC, c/ Marie Curie 2, 28049, Madrid, Spain. marisela.velez@icp.csic.es.
authors.forename: Marisela
authors.initials: M
authors.lastname: Vélez
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D055664:Electrochemical Techniques; D004566:Electrodes; D006859:Hydrogen / Q000737:chemistry* / Q000378:metabolism; D006864:Hydrogenase / Q000737:chemistry / Q000378:metabolism; D008051:Lipid Bilayers / Q000737:chemistry; D018625:Microscopy, Atomic Force; D025261:Mitochondrial Proton-Translocating ATPases / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D058525:Quartz Crystal Microbalance Techniques
chemical_list: D008051:Lipid Bilayers; D006859:Hydrogen; D000255:Adenosine Triphosphate; C058560:nickel-iron-selenium hydrogenase; D006864:Hydrogenase; C474451:F1F0-ATP synthase; D025261:Mitochondrial Proton-Translocating ATPases
keywords: bioelectrochemistry; biophysics; immobilization; membrane proteins; proton transport