title: Genome sequence of a divergent Colombian isolate of potato virus V (PVV) infecting Solanum phureja.
abstract: Deep sequencing analysis of the transcriptome of a Solanum phureja cv. Criolla Colombia plant with symptoms typical of a virus disease revealed an infection with potato virus V (PVV). The PVV-phureja genome comprises 9904 nt, exhibits 83% nucleotide identity with currently fully sequenced PVV isolates and contains one large ORF that codes for a polyprotein of 3065 residues flanked by 5' and 3' UTR of 217 and 448 nt, respectively. Phylogenetic analysis of the PVV-phureja polyprotein indicates that it is divergent with respect to most PVV isolates. This is the first complete PVV genome of an isolate infecting a host different to S. tuberosum and, to this date, the only one from the South American Andes.
authors.forename: P
authors.initials: P
authors.lastname: Gutiérrez
authors.forename: H Jaramillo
authors.initials: HJ
authors.lastname: Mesa
authors.forename: M
authors.initials: M
authors.lastname: Marín Montoya
mesh_terms: D001483:Base Sequence; D003105:Colombia; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017800:Potyvirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D032322:Solanum / Q000821:virology*
keywords: potato virus V; potyvirus; Solanum phureja; next generation sequencing.