title: Development of dual-emission ratiometric probe-based on fluorescent silica nanoparticle and CdTe quantum dots for determination of glucose in beverages and human body fluids.
abstract: A novel dual emission ratiometric fluorescence probe for determination of glucose has been developed. The reference dye fluorescence isothiocyanate (FITC) has been encapsulated in the silica nanoparticles and then the red emission CdTe QDs were grafted on the surface of the silica particles to obtain the fluorescence probe. With glucose and dopamine as substrates, the glucose level was proportional to the fluorescence ratio change of above probe caused by dopamine oxidation, which was produced via bienzyme catalysis (glucose oxidase and horseradish peroxidase). The established approach was sensitive and selective, and has been applied to determine the glucose in beverage, urine and serum samples. The average recoveries of the glucose at various spiking levels ranged from 95.5% to 108.9% with relative standard deviations from 1.5% to 4.3%. The results provided a clue to develop sensors for rapid determination of the target analytes from complex matrices.
authors.affiliation: Analytical & Testing Center of Beijing Normal University, Beijing 100875, China; College of Chemistry and Environmental Engineering, Shanxi Datong University, Datong 037009, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhai
authors.affiliation: Analytical & Testing Center of Beijing Normal University, Beijing 100875, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Feng
authors.affiliation: Analytical & Testing Center of Beijing Normal University, Beijing 100875, China.
authors.forename: Lingyu
authors.initials: L
authors.lastname: Dong
authors.affiliation: Analytical & Testing Center of Beijing Normal University, Beijing 100875, China.
authors.forename: Liyun
authors.initials: L
authors.lastname: Wang
authors.affiliation: Analytical & Testing Center of Beijing Normal University, Beijing 100875, China.
authors.forename: Xiangfeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Analytical & Testing Center of Beijing Normal University, Beijing 100875, China.
authors.forename: Hailing
authors.initials: H
authors.lastname: Liu
authors.affiliation: Analytical & Testing Center of Beijing Normal University, Beijing 100875, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Analytical & Testing Center of Beijing Normal University, Beijing 100875, China.
authors.forename: Luan
authors.initials: L
authors.lastname: Chen
authors.affiliation: Analytical & Testing Center of Beijing Normal University, Beijing 100875, China. Electronic address: xiemx@bnu.edu.cn.
authors.forename: MengXia
authors.initials: M
authors.lastname: Xie
mesh_terms: D001628:Beverages / Q000032:analysis*; D001786:Blood Glucose / Q000032:analysis; D001826:Body Fluids; D019187:Cadmium Compounds / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D005947:Glucose / Q000032:analysis*; D006801:Humans; D045663:Quantum Dots / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D013691:Tellurium / Q000737:chemistry*; D014556:Urine / Q000737:chemistry
chemical_list: D001786:Blood Glucose; D019187:Cadmium Compounds; D005456:Fluorescent Dyes; D012822:Silicon Dioxide; D005947:Glucose; D013691:Tellurium; C028337:cadmium telluride
keywords: Beverage; Dual-emission ratiometric fluorescence probe; FITC; Glucose; Quantum dots; Silica nanoparticles