title: Frapid: achieving full automation of FRAP for chemical probe validation.
abstract: Fluorescence Recovery After Photobleaching (FRAP) is an established method for validating chemical probes against the chromatin reading bromodomains, but so far requires constant human supervision. Here, we present Frapid, an automated open source code implementation of FRAP that fully handles cell identification through fuzzy logic analysis, drug dispensing with a custom-built fluid handler, image acquisition & analysis, and reporting. We successfully tested Frapid on 3 bromodomains as well as on spindlin1 (SPIN1), a methyl lysine binder, for the first time.
authors.affiliation: Target Discovery Institute, NDMRB, University of Oxford, Oxford, OX3 7FZ, UK; Botnar Research Centre, NDORMS, University of Oxford, Oxford, OX3 7LD, UK.
authors.forename: Clarence
authors.initials: C
authors.lastname: Yapp
authors.affiliation: Target Discovery Institute, NDMRB, University of Oxford, Oxford, OX3 7FZ, UK.
authors.forename: Catherine
authors.initials: C
authors.lastname: Rogers
authors.affiliation: Structural Genomics Consortium, ORCRB, University of Oxford, Oxford, OX3 7DQ, UK.
authors.forename: Pavel
authors.initials: P
authors.lastname: Savitsky
authors.affiliation: Botnar Research Centre, NDORMS, University of Oxford, Oxford, OX3 7LD, UK.
authors.forename: Martin
authors.initials: M
authors.lastname: Philpott
authors.affiliation: Target Discovery Institute, NDMRB, University of Oxford, Oxford, OX3 7FZ, UK.
authors.forename: Susanne
authors.initials: S
authors.lastname: Müller
keywords: (100.3008) Image recognition, algorithms and filters; (150.0150) Machine vision; (150.5758) Robotic and machine control; (170.1530) Cell analysis; (170.1790) Confocal microscopy; (170.2520) Fluorescence microscopy