title: Shape and size engineered cellulosic nanomaterials as broad spectrum anti-microbial compounds.
abstract: Oxidized celluloses have been used for decades as antimicrobial wound gauzes and surgical cotton. We now report the successful synthesis of a next generation narrow size range (25-35nm) spherical shaped nanoparticles of 2,3,6-tricarboxycellulose based on cellulose I structural features, for applications as new antimicrobial materials. This study adds to our previous study of 6-carboxycellulose. A wide range of bacteria such as Escherichia coli, Staphloccocus aureus, Bacillus subtilis and Mycobacterium tuberculosis (non-pathogenic as well as pathogenic strains) were affected by these polymers in in vitro studies. Activity against Mycobacteria were noted at high concentrations (MIC99 values 250-1000μg/ml, as compared to anti-TB drug Isoniazid 0.3μg/ml). However, the broad spectrum activity of oxidized celluloses and their nanoparticles against a wide range of bacteria, including Mycobacteria, show that these materials are promising new biocompatible and biodegradable drug delivery vehicles wherein they can play the dual role of being a drug encapsulant as well as a broad spectrum anti-microbial and anti-TB drug.
authors.affiliation: CSIR-National Chemical Laboratory, Polymer Science & Engineering Division, Dr. Homi Bhabha Road, Pune 411008, India.
authors.forename: Priyanka R
authors.initials: PR
authors.lastname: Sharma
authors.affiliation: CSIR-National Chemical Laboratory, Combi-Chem Lab (Organic Chemistry Division), Dr. Homi Bhabha Road, Pune 411008, India.
authors.forename: Sunil
authors.initials: S
authors.lastname: Kamble
authors.affiliation: CSIR-National Chemical Laboratory, Combi-Chem Lab (Organic Chemistry Division), Dr. Homi Bhabha Road, Pune 411008, India.
authors.forename: Dhiman
authors.initials: D
authors.lastname: Sarkar
authors.affiliation: CSIR-Institute of Genomics and Integrative Biology, Mathura Road, New Delhi 110 020, India.
authors.forename: Amitesh
authors.initials: A
authors.lastname: Anand
authors.affiliation: CSIR-National Chemical Laboratory, Polymer Science & Engineering Division, Dr. Homi Bhabha Road, Pune 411008, India. Electronic address: aj.varma@ncl.res.in.
authors.forename: Anjani J
authors.initials: AJ
authors.lastname: Varma
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D002483:Cellulose, Oxidized / Q000737:chemistry* / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology
chemical_list: D000900:Anti-Bacterial Agents; D002483:Cellulose, Oxidized; C585137:6-carboxycellulose
keywords: Anti-microbial; Cellulose; Spherical nanoparticles; Tricarboxycellulose