title: Genome Sequencing and Annotation of Mycobacterium tuberculosis PR08 strain.
abstract: Mycobacterium tuberculosis is an acid fast bacterial species in the family Mycobacteriaceae and is the causative agent of most cases of tuberculosis. Here, we report the genomic features of Mycobacterium tuberculosis isolated from the cerebrospinal fluid (CSF) of a patient diagnosed with both pulmonary and extrapulmonary tuberculosis (TB). The isolated strain was identified as Mycobacterium tuberculosis PR08 (MTB PR08). Genomic DNA of the MTB PR08 strain was extracted and subjected to whole genome sequencing using MiSeq (Illumina, CA,USA). The draft genome size of MTB PR08 strain is 4,292,364 bp with a G + C content of 65.2%. This strain was annotated to have 4723 genes and 48 RNAs. This whole genome shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession number CP010895.
authors.affiliation: Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA (UiTM), Malaysia.
authors.forename: Mohammad Maaruf
authors.initials: MM
authors.lastname: Jaafar
authors.affiliation: Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA (UiTM), Malaysia.
authors.forename: Mohd Zakihalani A
authors.initials: MZ
authors.lastname: Halim
authors.affiliation: Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA (UiTM), Malaysia.
authors.forename: Mohamad Izwan
authors.initials: MI
authors.lastname: Ismail
authors.affiliation: Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA (UiTM), Malaysia.
authors.forename: Lee Lian
authors.initials: LL
authors.lastname: Shien
authors.affiliation: Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA (UiTM), Malaysia; Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Malaysia.
authors.forename: Teh Lay
authors.initials: TL
authors.lastname: Kek
authors.affiliation: Faculty of Medicine, University of Malaya, Malaysia.
authors.forename: Ngeow Yun
authors.initials: NY
authors.lastname: Fong
authors.affiliation: School of Health Sciences, Health Campus, Universiti Sains Malaysia, Malaysia; Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Malaysia.
authors.forename: Norazmi Mohd
authors.initials: NM
authors.lastname: Nor
authors.affiliation: School of Health Sciences, Health Campus, Universiti Sains Malaysia, Malaysia.
authors.forename: Zainul Fadziruddin
authors.initials: ZF
authors.lastname: Zainuddin
authors.affiliation: Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia, Malaysia.
authors.forename: Tang Thean
authors.initials: TT
authors.lastname: Hock
authors.affiliation: School of Biological Sciences, Universiti Sains Malaysia, Malaysia.
authors.forename: Mohd Nazalan Mohd
authors.initials: MN
authors.lastname: Najimudin
authors.affiliation: Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA (UiTM), Malaysia.
authors.forename: Mohd Zaki
authors.initials: MZ
authors.lastname: Salleh
keywords: CSF; Extrapulmonary; Genome; Mycobacterium tuberculosis