title: Ionic liquids as a key medium for efficient extraction of copper complexes from chia seeds (Salvia hispanica L.).
abstract: Due to insufficient information, the aim of study was to concern on the optimization of extraction procedure of selected metal complexes with flavonoids from chia seeds. Evaluation of the amount of elements in compound, not only their total concentration content, is highly important due to the fact, that only a part from total content of metal is absorbed by human body. At the beginning the total amount of elements in chia seeds was established as 14.51±0.42 µg g(-1) for copper, 57.44±1.23 µg g(-1) for manganese, 81.12±1.89 µg g(-1) for zinc and 0.35±0.13 µg g(-1) for cobalt. After the most suitable solvent was established, effects of several parameters on the efficiency of metal extraction were studied. Solvent concentration, solid-solvent ratio, extraction method, extraction time and temperature have been investigated as independent variables. The optimal extraction conditions included vortexing during 20 min in 50°C, using an ionic liquid (1-butyl-3-methylimidazolium bromide) as an extractant, with solid-solvent ratio of 1:20. The determination of total and extractable amount of metals in chia seeds was carried out by standalone ICP MS. In addition, a complementary analysis of extracted metal complexes was performed using SEC-ICP MS method. It was confirmed that the ionic liquid is able to extract different copper complexes in comparison with commonly used solvents. The study indicated that extraction by using an ionic liquid has been successfully applied for determination of metals and metal complexes in chia seeds.
authors.affiliation: Chair of Analytical Chemistry, Faculty of Chemistry, Warsaw University of Technology, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Wojcieszek
authors.affiliation: Chair of Analytical Chemistry, Faculty of Chemistry, Warsaw University of Technology, Poland.
authors.forename: Dominik
authors.initials: D
authors.lastname: Popowski
authors.affiliation: Chair of Analytical Chemistry, Faculty of Chemistry, Warsaw University of Technology, Poland. Electronic address: lenka@ch.pw.edu.pl.
authors.forename: Lena
authors.initials: L
authors.lastname: Ruzik
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D003300:Copper / Q000032:analysis; D005419:Flavonoids / Q000737:chemistry; D007093:Imidazoles / Q000737:chemistry; D052578:Ionic Liquids / Q000737:chemistry*; D013058:Mass Spectrometry; D009942:Organometallic Compounds / Q000032:analysis / Q000302:isolation & purification*; D027544:Salvia / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
chemical_list: C558137:1-n-butyl-3-methylimidazolium bromide; D005419:Flavonoids; D007093:Imidazoles; D052578:Ionic Liquids; D009942:Organometallic Compounds; D012997:Solvents; D003300:Copper
keywords: Chia seeds; Copper complexes; Inductively coupled plasma-mass spectrometry; Ionic liquids