title: Identification QTLs Controlling Genes for Se Uptake in Lentil Seeds.
abstract: Lentil (Lens culinaris Medik.) is an excellent source of protein and carbohydrates and is also rich in essential trace elements for the human diet. Selenium (Se) is an essential micronutrient for human health and nutrition, providing protection against several diseases and regulating important biological systems. Dietary intake of 55 μg of Se per day is recommended for adults, with inadequate Se intake causing significant health problems. The objective of this study was to identify and map quantitative trait loci (QTL) of genes controlling Se accumulation in lentil seeds using a population of 96 recombinant inbred lines (RILs) developed from the cross "PI 320937" × "Eston" grown in three different environments for two years (2012 and 2013). Se concentration in seed varied between 119 and 883 μg/kg. A linkage map consisting of 1,784 markers (4 SSRs, and 1,780 SNPs) was developed. The map spanned a total length of 4,060.6 cM, consisting of 7 linkage groups (LGs) with an average distance of 2.3 cM between adjacent markers. Four QTL regions and 36 putative QTL markers, with LOD scores ranging from 3.00 to 4.97, distributed across two linkage groups (LG2 and LG5) were associated with seed Se concentration, explaining 6.3-16.9% of the phenotypic variation.
authors.affiliation: Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, Izmir, Turkey.
authors.forename: Duygu
authors.initials: D
authors.lastname: Ates
authors.affiliation: Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, Izmir, Turkey.
authors.forename: Tugce
authors.initials: T
authors.lastname: Sever
authors.affiliation: Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, Izmir, Turkey.
authors.forename: Secil
authors.initials: S
authors.lastname: Aldemir
authors.affiliation: Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, Izmir, Turkey.
authors.forename: Bulent
authors.initials: B
authors.lastname: Yagmur
authors.affiliation: Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, Izmir, Turkey.
authors.forename: Hulya Yilmaz
authors.initials: HY
authors.lastname: Temel
authors.affiliation: Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, Izmir, Turkey.
authors.forename: Hilal Betul
authors.initials: HB
authors.lastname: Kaya
authors.affiliation: Department of Field Crops, Faculty of Agriculture, Cukurova University, Adana, Turkey.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Alsaleh
authors.affiliation: Department of Field Crops, Faculty of Agriculture, Harran University, Sanlı Urfa, Turkey.
authors.forename: Abdullah
authors.initials: A
authors.lastname: Kahraman
authors.affiliation: Department of Field Crops, Faculty of Agriculture, Cukurova University, Adana, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Ozkan
authors.affiliation: Crop Development Centre, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
authors.forename: Albert
authors.initials: A
authors.lastname: Vandenberg
authors.affiliation: Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, Izmir, Turkey.
authors.forename: Bahattin
authors.initials: B
authors.lastname: Tanyolac
mesh_terms: D017343:Genes, Plant; D008040:Genetic Linkage; D027806:Lens Plant / Q000196:embryology* / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D012639:Seeds / Q000235:genetics / Q000378:metabolism*; D012643:Selenium / Q000378:metabolism*
chemical_list: D012643:Selenium