title: Anti-inflammatory effects of royal poinciana through inhibition of toll-like receptor 4 signaling pathway.
abstract: Inflammation is part of the non-specific immune response that occurs in reaction to any type of bodily injury. In some disorders the inflammatory process, which under normal conditions is self-limiting, becomes continuous and chronic inflammatory diseases develop subsequently including cardiovascular diseases, diabetes, cancer etc. Barks of Delonix regia is used traditionally in the treatment of inflammatory diseases. Therefore, in this study we evaluated the therapeutic potential of D. regia ethanol extract and its active constituent β-Elemene with special interest in inflammation model using standard in vivo anti-inflammatory models: Carrageenan-induced paw edema, Cotton pellet granuloma, and Acetic acid-induced vascular permeability. To explicate the mechanism of action for the possible anti-inflammatory activity, we determined the level of major inflammatory mediators (NO, iNOS, COX-2-dependent prostaglandin E2 or PGE2), and pro-inflammatory cytokines (TNF-a, IL-1b, IL-6, and IL-12). Additionally, we determined the toll-like receptor 4 (TLR4), Myeloid differentiation primary response gene 88 (MyD88), by mRNA expression in drug treated LPS-induced murine macrophage model. To explore the mechanism of anti-inflammatory activity, we evaluated expression of c-Jun N-terminal kinases (JNK), nuclear factor kappa-B cells (NF-kB), and NF-kB inhibitor alpha (IK-Ba). Furthermore, we determined the acute and sub-acute toxicity of D. regia extract in BALB/c mice. This study established a significant anti-inflammatory activity of D. regia extract and β-Elemene along with the inhibition of TNF-a, IL-1b, IL-6 and IL-12 expressions. Further, the expression of TLR4, NF-kBp65, MyD88, iNOS and COX-2 molecules were reduced in drug-treated groups, but not in the LPS-stimulated untreated or control groups, Thus, our results collectively indicated that the D. regia extract and β-Elemene can efficiently inhibit inflammation.
authors.affiliation: American International Medical University, Bosejour Road, Gros Islet, Saint Lucia.
authors.forename: Satyajit
authors.initials: S
authors.lastname: Patra
authors.affiliation: Department of Biotechnology, School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu, India.
authors.forename: Meenakshi Sundaram
authors.initials: MS
authors.lastname: Muthuraman
authors.affiliation: Department of Biotechnology, School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu, India.
authors.forename: M
authors.initials: M
authors.lastname: Meenu
authors.affiliation: Department of Biotechnology, School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu, India.
authors.forename: Padma
authors.initials: P
authors.lastname: Priya
authors.affiliation: Centre for Advanced Research in Indian System of Medicine, SASTRA University, Thanjavur, Tamil Nadu, India.
authors.forename: Brindha
authors.initials: B
authors.lastname: Pemaiah
mesh_terms: D019342:Acetic Acid; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D002199:Capillary Permeability / Q000187:drug effects; D002351:Carrageenan; D051546:Cyclooxygenase 2; D016207:Cytokines; D004195:Disease Models, Animal; D004487:Edema / Q000188:drug therapy*; D007887:Fabaceae / Q000276:immunology; D006099:Granuloma / Q000188:drug therapy*; D018836:Inflammation Mediators; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D012717:Sesquiterpenes / Q000627:therapeutic use*; D015398:Signal Transduction / Q000187:drug effects; D051197:Toll-Like Receptor 4 / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D018836:Inflammation Mediators; D010936:Plant Extracts; D012717:Sesquiterpenes; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; C445979:beta-elemene; D002351:Carrageenan; D051546:Cyclooxygenase 2; D019342:Acetic Acid
keywords: Analgesic; Anti-inflammatory; Antipyretic; Cyclooxygenase II; Delonix regia; β-Elemene