title: Possible Effect of Muscle-relaxant Anaesthetics on Invasion, Adhesion and Migration of Breast Cancer Cells.
abstract: BACKGROUND
  Aggressive surgical removal of the primary tumour is the preferred treatment, but with tumour progression, some tumours cannot be completely removed surgically. Anaesthetics are administered to facilitate surgery. However, anaesthetics act as a potential factor in tumour recurrence or metastasis.
  
  
  METHODS
  Normal breast cells and cancer breast cells were treated with different doses of muscle-relaxant anaesthetics. The effects on breast cancer cell invasion, adhesion and migration of these anaesthetics were then investigated using in vitro models.
  
  
  RESULTS
  With increasing dose of rocuronium bromide and suxamethonium chloride CRS, the number of MCF-10A and MCF-7 cells, but not that of MDA-MB-231 cells, decreased. There was almost no difference in the number of cells when the three cell lines were treated with different doses of vecuronium bromide. The study also demonstrated that rocuronium bromide promoted the invasion, adhesion and growth of MDA-231 cells, while suxamethonium chloride CRS had no effect. Interestingly, vecuronium bromide did not affect the motility and invasion of breast cancer cells significantly.
  
  
  CONCLUSIONS
  An understanding of the effect of anaesthetics and their impact on tumour metastasis is important, thus using an appropriate aesthetic strategy could improve long-term survival in some patients.
authors.affiliation: Department of Anesthesiology, Yuhuangding Hospital, Medical College, Qingdao University, Yantai, P.R. China Cardiff-China Medical Research Collaborative, Cardiff University School of Medicine, Heath Park, Cardiff, U.K.
authors.forename: Aihua
authors.initials: A
authors.lastname: Jiang
authors.affiliation: Cardiff-China Medical Research Collaborative, Cardiff University School of Medicine, Heath Park, Cardiff, U.K. Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Beijing Key Laboratory for Cancer Invasion and Metastasis Research, Cancer Institute of Capital Medical University, Beijing, P.R. China Capital Medical University-Cardiff University Joint Centre for Biomedical Research, Beijing International Cooperation Base for Science and Technology on China-UK Cancer Research, Capital Medical University, Beijing, P.R. China.
authors.forename: Huishan
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Cardiff-China Medical Research Collaborative, Cardiff University School of Medicine, Heath Park, Cardiff, U.K.
authors.forename: Jun
authors.initials: J
authors.lastname: Cai
authors.affiliation: Cardiff-China Medical Research Collaborative, Cardiff University School of Medicine, Heath Park, Cardiff, U.K. jiangw@cf.ac.uk.
authors.forename: Wen G
authors.initials: WG
authors.lastname: Jiang
mesh_terms: D000732:Androstanols / Q000633:toxicity*; D001943:Breast Neoplasms / Q000473:pathology*; D002448:Cell Adhesion / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D018450:Disease Progression; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D009361:Neoplasm Invasiveness; D009467:Neuromuscular Depolarizing Agents / Q000633:toxicity*; D018570:Risk Assessment; D000077123:Rocuronium; D013390:Succinylcholine / Q000633:toxicity*; D014673:Vecuronium Bromide / Q000633:toxicity
chemical_list: D000732:Androstanols; D009467:Neuromuscular Depolarizing Agents; D014673:Vecuronium Bromide; D013390:Succinylcholine; D000077123:Rocuronium
keywords: Anaesthetics; breast cancer; invasion; metastasis; suxamethonium chloride CRS; vecuronium bromide