title: Purification of mumps virus particles of high viability.
abstract: Mumps is a highly infectious viral disease of humans with a wide array of clinical manifestations ranging from painful swelling of the salivary glands to meningitis and encephalitis. Despite the clinical importance of mumps virus, most of what is known of its biological properties comes from studies using supernatants from virus infected cell cultures, which contain substantial levels of host cell derived debris and biologically active substances such as cytokines, transcription factors and secreted virus proteins. These contaminants complicate interpretation of studies of virus replication, virus-host interactions and in vivo virulence. Here we describe a protocol for concentration of the virus from cell culture supernatants followed by gradient purification, resulting in attainment of high titer live virus of high purity.
authors.affiliation: Food and Drug Administration, Center for Biologics Evaluation and Research, 10903 New Hampshire Avenue, Silver Spring, MD 20993, United States.
authors.forename: Huosheng
authors.initials: H
authors.lastname: Chen
authors.affiliation: Food and Drug Administration, Center for Biologics Evaluation and Research, 10903 New Hampshire Avenue, Silver Spring, MD 20993, United States.
authors.forename: Laurie
authors.initials: L
authors.lastname: Ngo
authors.affiliation: Food and Drug Administration, Center for Biologics Evaluation and Research, 10903 New Hampshire Avenue, Silver Spring, MD 20993, United States.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Petrovskaya
authors.affiliation: Food and Drug Administration, Center for Biologics Evaluation and Research, 10903 New Hampshire Avenue, Silver Spring, MD 20993, United States.
authors.forename: Yamei
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Food and Drug Administration, Center for Biologics Evaluation and Research, 10903 New Hampshire Avenue, Silver Spring, MD 20993, United States.
authors.forename: Majid
authors.initials: M
authors.lastname: Laassri
authors.affiliation: Food and Drug Administration, Center for Biologics Evaluation and Research, 10903 New Hampshire Avenue, Silver Spring, MD 20993, United States. Electronic address: steven.rubin@fda.hhs.gov.
authors.forename: Steven
authors.initials: S
authors.lastname: Rubin
mesh_terms: D006801:Humans; D050296:Microbial Viability; D009109:Mumps virus / Q000302:isolation & purification* / Q000648:ultrastructure; D012367:RNA, Viral; D017422:Sequence Analysis, DNA; D014461:Ultracentrifugation; D014771:Virion / Q000302:isolation & purification* / Q000648:ultrastructure
chemical_list: D012367:RNA, Viral
keywords: Mumps virus; Particles; Potassium tartrate; Purification; Ultracentrifugation