title: Mouse redox histology using genetically encoded probes.
abstract: Mapping the in vivo distribution of endogenous oxidants in animal tissues is of substantial biomedical interest. Numerous health-related factors, including diet, physical activity, infection, aging, toxins, or pharmacological intervention, may cause redox changes. Tools are needed to pinpoint redox state changes to particular organs, tissues, cell types, and subcellular organelles. We describe a procedure that preserves the in vivo redox state of genetically encoded redox biosensors within histological tissue sections, thus providing "redox maps" for any tissue and comparison of interest. We demonstrate the utility of the technique by visualizing endogenous redox differences and changes in the context of tumor growth, inflammation, embryonic development, and nutrient starvation.
authors.affiliation: Division of Redox Regulation, DKFZ-ZMBH (German Cancer Research Center-Center for Molecular Biology of the University of Heidelberg) Alliance, DKFZ, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany.
authors.forename: Yuuta
authors.initials: Y
authors.lastname: Fujikawa
authors.affiliation: Division of Redox Regulation, DKFZ-ZMBH (German Cancer Research Center-Center for Molecular Biology of the University of Heidelberg) Alliance, DKFZ, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany.
authors.forename: Leticia P
authors.initials: LP
authors.lastname: Roma
authors.affiliation: Division of Redox Regulation, DKFZ-ZMBH (German Cancer Research Center-Center for Molecular Biology of the University of Heidelberg) Alliance, DKFZ, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany.
authors.forename: Mirko C
authors.initials: MC
authors.lastname: Sobotta
authors.affiliation: Joint Division Molecular Metabolic Control, DKFZ-ZMBH Alliance, DKFZ Heidelberg, Center for Molecular Biology (ZMBH) and University Hospital, University of Heidelberg, 69120 Heidelberg, Germany.
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Rose
authors.affiliation: Joint Division Molecular Metabolic Control, DKFZ-ZMBH Alliance, DKFZ Heidelberg, Center for Molecular Biology (ZMBH) and University Hospital, University of Heidelberg, 69120 Heidelberg, Germany. Institute for Diabetes and Cancer (IDC) and Joint Heidelberg-IDC Translational Diabetes Program, Heidelberg University Hospital, Helmholtz Center Munich, 85764 Neuherberg, Germany.
authors.forename: Mauricio Berriel
authors.initials: MB
authors.lastname: Diaz
authors.affiliation: Institute of Clinical Neuroimmunology, Ludwig-Maximilians-Universität München, 81377 Munich, Germany.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Locatelli
authors.affiliation: Institute of Clinical Neuroimmunology, Ludwig-Maximilians-Universität München, 81377 Munich, Germany. Institute of Neuronal Cell Biology, Technical University of Munich, 80802 Munich, Germany.
authors.forename: Michael O
authors.initials: MO
authors.lastname: Breckwoldt
authors.affiliation: Institute of Neuronal Cell Biology, Technical University of Munich, 80802 Munich, Germany. Munich Cluster for Systems Neurology (SyNergy), 81377 Munich, Germany. German Center for Neurodegenerative Diseases (DZNE), 81377 Munich, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Misgeld
authors.affiliation: Institute of Clinical Neuroimmunology, Ludwig-Maximilians-Universität München, 81377 Munich, Germany. Munich Cluster for Systems Neurology (SyNergy), 81377 Munich, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Kerschensteiner
authors.affiliation: Joint Division Molecular Metabolic Control, DKFZ-ZMBH Alliance, DKFZ Heidelberg, Center for Molecular Biology (ZMBH) and University Hospital, University of Heidelberg, 69120 Heidelberg, Germany. Institute for Diabetes and Cancer (IDC) and Joint Heidelberg-IDC Translational Diabetes Program, Heidelberg University Hospital, Helmholtz Center Munich, 85764 Neuherberg, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Herzig
authors.affiliation: Core Facility Tumor Models, DKFZ, 69120 Heidelberg, Germany.
authors.forename: Karin
authors.initials: K
authors.lastname: Müller-Decker
authors.affiliation: Division of Redox Regulation, DKFZ-ZMBH (German Cancer Research Center-Center for Molecular Biology of the University of Heidelberg) Alliance, DKFZ, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany. t.dick@dkfz.de.
authors.forename: Tobias P
authors.initials: TP
authors.lastname: Dick
mesh_terms: D000818:Animals; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D057054:Molecular Imaging / Q000379:methods*; D015335:Molecular Probes / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D019076:Transgenes
chemical_list: D015335:Molecular Probes