title: Detection of ureolytic activity of bacterial strains isolated from entomopathogenic nematodes using infrared spectroscopy.
abstract: The pathogenicity of entomopathogenic nematodes (EPNs) depends directly on the presence of bacteria in the nematode digestive tracts. Based on 16S rRNA and MALDI-TOF analyses 20 isolated bacteria were assigned to 10 species with 10 isolates classified as Pseudomonas ssp. Six strains (30%) show ureolytic activity on Christensen medium. Spectroscopic analysis of the strains showed that the ureolytic activity is strongly correlated with the following wavenumbers: 935 cm(-1) in window W4, which carries information about the bacterial cell wall construction and 1158 cm(-1) in window W3 which corresponds to proteins in bacterial cell. A logistic regression model designed on the basis of the selected wavenumbers differentiates ureolytic from non-ureolytic bacterial strains with an accuracy of 100%. Spectroscopic studies and mathematical analyses made it possible to differentiate EPN-associated Pseudomonas sp. strains from clinical Pseudomonas aeruginosa PAO1. These results suggest, that infrared spectra of EPN-associated Pseudomonas sp. strains may reflect its adaptation to the host.
authors.affiliation: Department of Microbiology, Jan Kochanowski University, Kielce, Poland.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Lechowicz
authors.affiliation: Department of Probability and Statistics, Jan Kochanowski University, Kielce, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Chrapek
authors.affiliation: Department of Microbiology, Jan Kochanowski University, Kielce, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Czerwonka
authors.affiliation: Department of Immunology of Infectious Diseases, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Korzeniowska-Kowal
authors.affiliation: Department of Immunology of Infectious Diseases, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Tobiasz
authors.affiliation: Division of Organic Chemistry, Jan Kochanowski University, Kielce, Poland.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Urbaniak
authors.affiliation: Department of Zoology and Biological Didactics, Jan Kochanowski University, Kielce, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Matuska-Lyzwa
authors.affiliation: Department of Microbiology, Jan Kochanowski University, Kielce, Poland.
authors.forename: Wieslaw
authors.initials: W
authors.lastname: Kaca
mesh_terms: D000818:Animals; D007313:Insecta / Q000469:parasitology*; D009348:Nematoda / Q000382:microbiology*; D011549:Pseudomonas / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014508:Urea / Q000378:metabolism*
chemical_list: D014508:Urea
keywords: Infrared spectroscopy; Pseudomonas; Urease