title: Characterization and comparison of post-natal rat Achilles tendon-derived stem cells at different development stages.
abstract: Tendon stem/progenitor cells (TSPCs) are a potential cell source for tendon tissue engineering. The striking morphological and structural changes of tendon tissue during development indicate the complexity of TSPCs at different stages. This study aims to characterize and compare post-natal rat Achilles tendon tissue and TSPCs at different stages of development. The tendon tissue showed distinct differences during development: the tissue structure became denser and more regular, the nuclei became spindle-shaped and the cell number decreased with time. TSPCs derived from 7 day Achilles tendon tissue showed the highest self-renewal ability, cell proliferation, and differentiation potential towards mesenchymal lineage, compared to TSPCs derived from 1 day and 56 day tissue. Microarray data showed up-regulation of several groups of genes in TSPCs derived from 7 day Achilles tendon tissue, which may account for the unique cell characteristics during this specific stage of development. Our results indicate that TSPCs derived from 7 day Achilles tendon tissue is a superior cell source as compared to TSPCs derived from 1 day and 56 day tissue, demonstrating the importance of choosing a suitable stem cell source for effective tendon tissue engineering and regeneration.
authors.affiliation: Centre for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, Hangzhou, 310058, China.|Zhejiang Provincial Key Lab for tissue engineering and regenerative medicine, 310000, Hangzhou, China.|Department of Integrative Medical Biology, Anatomy, Umeå University, Umeå, 90187, Sweden.
authors.forename: Jialin
authors.initials: J
authors.lastname: Chen
authors.affiliation: Centre for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, Hangzhou, 310058, China.|Zhejiang Provincial Key Lab for tissue engineering and regenerative medicine, 310000, Hangzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Centre for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, Hangzhou, 310058, China.|Zhejiang Provincial Key Lab for tissue engineering and regenerative medicine, 310000, Hangzhou, China.
authors.forename: Zeyu
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Centre for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, Hangzhou, 310058, China.|Zhejiang Provincial Key Lab for tissue engineering and regenerative medicine, 310000, Hangzhou, China.|Department of Orthopaedics, Second Affiliated Hospital, Zhejiang University, Hangzhou, 310009, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhu
authors.affiliation: Centre for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, Hangzhou, 310058, China.|Zhejiang Provincial Key Lab for tissue engineering and regenerative medicine, 310000, Hangzhou, China.|Department of Orthopaedics, Second Affiliated Hospital, Zhejiang University, Hangzhou, 310009, China.
authors.forename: Weiliang
authors.initials: W
authors.lastname: Shen
authors.affiliation: Department of Orthopaedics, Second Affiliated Hospital, Zhejiang University, Hangzhou, 310009, China.
authors.forename: Jisheng
authors.initials: J
authors.lastname: Ran
authors.affiliation: Centre for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, Hangzhou, 310058, China.|Zhejiang Provincial Key Lab for tissue engineering and regenerative medicine, 310000, Hangzhou, China.|Department of Orthopaedics, Second Affiliated Hospital, Zhejiang University, Hangzhou, 310009, China.
authors.forename: Qiaomei
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Centre for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, Hangzhou, 310058, China.|Zhejiang Provincial Key Lab for tissue engineering and regenerative medicine, 310000, Hangzhou, China.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Gong
authors.affiliation: Department of Integrative Medical Biology, Anatomy, Umeå University, Umeå, 90187, Sweden.
authors.forename: Ludvig J
authors.initials: LJ
authors.lastname: Backman
authors.affiliation: Centre for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, Hangzhou, 310058, China.|Zhejiang Provincial Key Lab for tissue engineering and regenerative medicine, 310000, Hangzhou, China.|China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, 310058, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Chen
authors.affiliation: Division of haematology and oncology, Shenzhen Children's Hospital, Shenzhen, 518038, China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Chen
authors.affiliation: Division of haematology and oncology, Shenzhen Children's Hospital, Shenzhen, 518038, China.
authors.forename: Feiqiu
authors.initials: F
authors.lastname: Wen
authors.affiliation: Centre for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, Hangzhou, 310058, China.|Zhejiang Provincial Key Lab for tissue engineering and regenerative medicine, 310000, Hangzhou, China.|Department of Sports Medicine, School of Medicine, Zhejiang University, Hangzhou, 310058, China.|China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, 310058, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Ouyang
mesh_terms: D000125:Achilles Tendon / Q000166:cytology* / Q000254:growth & development / Q000648:ultrastructure; D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D049109:Cell Proliferation / Q000235:genetics / Q000502:physiology*; D000066673:Cell Self Renewal / Q000502:physiology; D002478:Cells, Cultured; D020869:Gene Expression Profiling / Q000379:methods; D018507:Gene Expression Regulation, Developmental; D063990:Gene Ontology; D046529:Microscopy, Electron, Transmission; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000378:metabolism / Q000502:physiology*; D013997:Time Factors