title: Rapid spread of influenza A(H1N1)pdm09 viruses with a new set of specific mutations in the internal genes in the beginning of 2015/2016 epidemic season in Moscow and Saint Petersburg (Russian Federation).
abstract: A dramatic increase of influenza activity in Russia since week 3 of 2016 significantly differs from previous seasons in terms of the incidence of influenza and acute respiratory infection (ARI) and in number of lethal cases. We performed antigenic analysis of 108 and whole-genome sequencing of 77 influenza A(H1N1)pdm09 viruses from Moscow and Saint Petersburg. Most of the viruses were antigenically related to the vaccine strain. Whole-genome analysis revealed a composition of specific mutations in the internal genes (D2E and M83I in NEP, E125D in NS1, M105T in NP, Q208K in M1, and N204S in PA-X) that probably emerged before the beginning of 2015/2016 epidemic season.
authors.affiliation: Research Institute of Influenza, Ministry of Healthcare of the Russian Federation, Laboratory of Molecular Virology and Genetic Engineering, WHO-recognized National Influenza Centre of Russian Federation, Saint Petersburg, Russia.
authors.forename: Andrey
authors.initials: A
authors.lastname: Komissarov
authors.affiliation: Research Institute of Influenza, Ministry of Healthcare of the Russian Federation, Laboratory of Molecular Virology and Genetic Engineering, WHO-recognized National Influenza Centre of Russian Federation, Saint Petersburg, Russia.
authors.forename: Artem
authors.initials: A
authors.lastname: Fadeev
authors.affiliation: Research Institute of Influenza, Ministry of Healthcare of the Russian Federation, Laboratory of Influenza Vaccines, Saint Petersburg, Russia.
authors.forename: Maria
authors.initials: M
authors.lastname: Sergeeva
authors.affiliation: Research Institute of Influenza, Ministry of Healthcare of the Russian Federation, Laboratory of Influenza Vaccines, Saint Petersburg, Russia.
authors.forename: Sergey
authors.initials: S
authors.lastname: Petrov
authors.affiliation: Research Institute of Influenza, Ministry of Healthcare of the Russian Federation, Laboratory of Molecular Virology and Genetic Engineering, WHO-recognized National Influenza Centre of Russian Federation, Saint Petersburg, Russia.
authors.forename: Kseniya
authors.initials: K
authors.lastname: Sintsova
authors.affiliation: Research Institute of Influenza, Ministry of Healthcare of the Russian Federation, Laboratory of Molecular Virology and Genetic Engineering, WHO-recognized National Influenza Centre of Russian Federation, Saint Petersburg, Russia.
authors.forename: Anna
authors.initials: A
authors.lastname: Egorova
authors.affiliation: Research Institute of Influenza, Ministry of Healthcare of the Russian Federation, Laboratory of Molecular Virology and Genetic Engineering, WHO-recognized National Influenza Centre of Russian Federation, Saint Petersburg, Russia.
authors.forename: Maria
authors.initials: M
authors.lastname: Pisareva
authors.affiliation: Research Institute of Influenza, Ministry of Healthcare of the Russian Federation, Laboratory of Molecular Virology and Genetic Engineering, WHO-recognized National Influenza Centre of Russian Federation, Saint Petersburg, Russia.
authors.forename: Zhanna
authors.initials: Z
authors.lastname: Buzitskaya
authors.affiliation: Research Institute of Influenza, Ministry of Healthcare of the Russian Federation, Laboratory of Molecular Virology and Genetic Engineering, Saint Petersburg, Russia.
authors.forename: Tamila
authors.initials: T
authors.lastname: Musaeva
authors.affiliation: Research Institute of Influenza, Ministry of Healthcare of the Russian Federation, Laboratory of Evolutionary Variability of Influenza Viruses, WHO-recognized National Influenza Centre of Russian Federation, Saint Petersburg, Russia.
authors.forename: Daria
authors.initials: D
authors.lastname: Danilenko
authors.affiliation: Research Institute of Influenza, Ministry of Healthcare of the Russian Federation, Laboratory of Evolutionary Variability of Influenza Viruses, WHO-recognized National Influenza Centre of Russian Federation, Saint Petersburg, Russia.
authors.forename: Nadezhda
authors.initials: N
authors.lastname: Konovalova
authors.affiliation: Research Institute of Influenza, Ministry of Healthcare of the Russian Federation, Laboratory of Evolutionary Variability of Influenza Viruses, WHO-recognized National Influenza Centre of Russian Federation, Saint Petersburg, Russia.
authors.forename: Polina
authors.initials: P
authors.lastname: Petrova
authors.affiliation: Research Institute of Influenza, Ministry of Healthcare of the Russian Federation, Department of IT, WHO-recognized National Influenza Centre of Russian Federation, Saint Petersburg, Russia.
authors.forename: Kirill
authors.initials: K
authors.lastname: Stolyarov
authors.affiliation: Research Institute of Influenza, Ministry of Healthcare of the Russian Federation, Laboratory of Biotechnology, WHO-recognized National Influenza Centre of Russian Federation, Saint Petersburg, Russia.
authors.forename: Elizaveta
authors.initials: E
authors.lastname: Smorodintseva
authors.affiliation: Federal Research Center of Epidemiology and Microbiology named after N. F. Gamaleya, WHO-recognized National Influenza Centre of Russian Federation, Moscow, Russia.
authors.forename: Elena
authors.initials: E
authors.lastname: Burtseva
authors.affiliation: Federal Research Center of Epidemiology and Microbiology named after N. F. Gamaleya, WHO-recognized National Influenza Centre of Russian Federation, Moscow, Russia.
authors.forename: Kirill
authors.initials: K
authors.lastname: Krasnoslobodtsev
authors.affiliation: Federal Research Center of Epidemiology and Microbiology named after N. F. Gamaleya, WHO-recognized National Influenza Centre of Russian Federation, Moscow, Russia.
authors.forename: Elena
authors.initials: E
authors.lastname: Kirillova
authors.affiliation: Research Institute of Influenza, Laboratory of Influenza and ARI Epidemiology, WHO-recognized National Influenza Centre of Russian Federation, Saint Petersburg, Russia.
authors.forename: Lyudmila
authors.initials: L
authors.lastname: Karpova
authors.affiliation: Research Institute of Influenza, Ministry of Healthcare of the Russian Federation, Laboratory of Evolutionary Variability of Influenza Viruses, WHO-recognized National Influenza Centre of Russian Federation, Saint Petersburg, Russia.
authors.forename: Mikhail
authors.initials: M
authors.lastname: Eropkin
authors.affiliation: Research Institute of Influenza, Ministry of Healthcare of the Russian Federation, Laboratory of Biotechnology, WHO-recognized National Influenza Centre of Russian Federation, Saint Petersburg, Russia.
authors.forename: Anna
authors.initials: A
authors.lastname: Sominina
authors.affiliation: Research Institute of Influenza, Ministry of Healthcare of the Russian Federation, Laboratory of Molecular Virology and Genetic Engineering, WHO-recognized National Influenza Centre of Russian Federation, Saint Petersburg, Russia.
authors.forename: Mikhail
authors.initials: M
authors.lastname: Grudinin
mesh_terms: D016679:Genome, Viral; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D007251:Influenza, Human / Q000453:epidemiology / Q000821:virology*; D009031:Moscow / Q000453:epidemiology; D009154:Mutation; D012426:Russia / Q000453:epidemiology; D012621:Seasons; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D014764:Viral Proteins
keywords: A(H1N1)pdm09; Russia; high-throughput nucleotide sequencing; influenza