title: Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry to differentiate between Candida albicans and Candida dubliniensis.
abstract: Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF-MS) analysis in conjunction with the direct formic acid (FA) sample processing method was evaluated for the ability to differentiate the closely related species of Candida albicans and Candida dubliniensis. The results showed that MALDI-TOF-MS, using the direct FA method, was reliable to differentiate between these species.
authors.affiliation: Department of Pathology, Anatomy and Cell Biology Sidney Kimmel Medical College at Thomas Jefferson University, Philadelphia, PA, USA. Electronic address: amity.roberts@jefferson.edu.
authors.forename: Amity L
authors.initials: AL
authors.lastname: Roberts
authors.affiliation: King Fahad Specialist Hospital, Dammam, Saudi Arabia. Electronic address: aqela4@hotmail.com.
authors.forename: Aqilah
authors.initials: A
authors.lastname: Alelew
authors.affiliation: Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, USA; Nebraska Public Health Laboratory, Omaha, NE, USA. Electronic address: piwen@unmc.edu.
authors.forename: Peter C
authors.initials: PC
authors.lastname: Iwen
mesh_terms: D002175:Candida / Q000145:classification*; D002176:Candida albicans / Q000145:classification*; D002177:Candidiasis / Q000175:diagnosis*; D006801:Humans; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
keywords: Candida dubliniensis; ITS1; ITS4; MALDI-TOF-MS