title: MiR-519a functions as a tumor suppressor in glioma by targeting the oncogenic STAT3 pathway.
abstract: Glioblastoma (GBM) is among the most aggressive primary brain tumors, with a median survival rate of 12-15 months. MicroRNAs have been implicated in GBM development as oncogenes or tumor suppressors. In this study, we demonstrated that miR-519a expression was frequently downregulated in GBM specimens and cell lines, and that low-levels miR-519a expression significantly correlated with poor outcomes associated with GBM. Analysis of The Cancer Genome Atlas also demonstrated that low miR-519a expression can predict poor clinical outcomes in classical and proneural GBM subtypes. Functionally, re-expression of miR-519a effectively reduced GBM cell proliferation, migration, and invasion. Mechanistically, we confirmed that the signal transducer and activator of transcription 3 (STAT3) 3'-UTR was a putative target of miR-519a, and that re-expression of STAT3 abrogated miR-519a function in GBM cells. Furthermore, we found that STAT3 expression negatively correlated with that of miR-519a in human GBM tissues. These results elucidated the prognostic value and tumor-suppressor role of miR-519a in GBM and further suggested it as a potential therapeutic target for GBM treatment.
authors.affiliation: Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Li
authors.initials: L
authors.lastname: Hong
authors.affiliation: Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.|Nanfang Neurology Research Institution, Nanfang Hospital, Guangzhou, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Ya-Wei
authors.affiliation: Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Hai
authors.affiliation: Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Qiang
authors.affiliation: Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Li
authors.initials: L
authors.lastname: Jun-Jie
authors.affiliation: Brain Tumor Research Center, The Hospital for Sick Children, Toronto, Canada.
authors.forename: Annie
authors.initials: A
authors.lastname: Huang
authors.affiliation: Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou, China. qisongtaosjwk@163.com.|Nanfang Neurology Research Institution, Nanfang Hospital, Guangzhou, China. qisongtaosjwk@163.com.|Nanfang Glioma Center, Guangzhou, China. qisongtaosjwk@163.com.
authors.forename: Qi
authors.initials: Q
authors.lastname: Song-Tao
authors.affiliation: Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou, China. lllu2000yun@gmail.com.|Nanfang Neurology Research Institution, Nanfang Hospital, Guangzhou, China. lllu2000yun@gmail.com.|Nanfang Glioma Center, Guangzhou, China. lllu2000yun@gmail.com.
authors.forename: Lu
authors.initials: L
authors.lastname: Yun-Tao
mesh_terms: D017209:Apoptosis / Q000502:physiology; D001921:Brain / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D002453:Cell Cycle / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D015331:Cohort Studies; D018450:Disease Progression; D005909:Glioblastoma / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000503:physiopathology; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D016019:Survival Analysis; D014158:Transcription, Genetic
chemical_list: C546194:MIRN519 microRNA, human; D035683:MicroRNAs; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human
keywords: C19MC; Glioblastoma; STAT3; miR-519a