title: 5-Fluorouracil induces apoptosis of colorectal cancer cells.
abstract: 5-Fluorouracil (5-FU) is widely used in chemotherapy for treatment of colorectal cancer. Leucine-rich repeat-containing G protein-coupled receptor (LGR) is known to participate in the occurrence and development of breast cancer by regulating the rebirth of tumor vessels. This study aimed to explore the proliferation and apoptosis of HCT116 colorectal cancer cells treated with 5-FU and related molecular mechanisms. 5-FU (20 μg/mL) was used to treat cultured HCT116 cells. An MTT test, flow cytometry, and colony formation assays were used to examine the proliferation and apoptosis of HCT116 cells. Western blotting was applied to detect the expression of the LGR4 protein in HCT116 cells. Small interference RNA or over-expression techniques were used to manipulate LGR4 expression in HCT116 cells and describe the proliferation and apoptosis of HCT116 treated with 5-FU. A dosage of 20 μg/mL 5-FU resulted in a significant decrease in the proliferation and apoptosis of HCT116 cells and significantly decreased expression levels of LGR4. The specific gene silence or over-expression of LGR4 in HCT116 cells increased and decreased the levels of apoptosis in HCT116, respectively. 5-FU induces apoptosis of colorectal cancer cells and inhibits proliferation by suppressing LGR4 proteins.
authors.affiliation: Department of Colorectal and Anal Surgery, First Affiliated Hospital of Jilin University, Changchun, China.
authors.forename: J T
authors.initials: JT
authors.lastname: Zhang
authors.affiliation: Department of Neonatology, First Affiliated Hospital of Jilin University, Changchun, China.
authors.forename: W L
authors.initials: WL
authors.lastname: Zhou
authors.affiliation: Department of Nerve Trauma Surgery, First Affiliated Hospital of Jilin University, Changchun, China.
authors.forename: C
authors.initials: C
authors.lastname: He
authors.affiliation: Department of Colorectal and Anal Surgery, First Affiliated Hospital of Jilin University, Changchun, China.
authors.forename: T
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Colorectal and Anal Surgery, First Affiliated Hospital of Jilin University, Changchun, China.
authors.forename: C Y
authors.initials: CY
authors.lastname: Li
authors.affiliation: Department of Colorectal and Anal Surgery, First Affiliated Hospital of Jilin University, Changchun, China.
authors.forename: L
authors.initials: L
authors.lastname: Wang
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis; D049109:Cell Proliferation / Q000187:drug effects; D015179:Colorectal Neoplasms / Q000378:metabolism*; D005472:Fluorouracil / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D045325:HCT116 Cells; D006801:Humans; D034741:RNA, Small Interfering / Q000494:pharmacology; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*
chemical_list: D000964:Antimetabolites, Antineoplastic; C411562:LGR4 protein, human; D034741:RNA, Small Interfering; D043562:Receptors, G-Protein-Coupled; D005472:Fluorouracil