title: Proteome Analyses ofStaphylococcus aureusBiofilm at Elevated Levels of NaCl.
abstract: Our studies demonstrate that sodium chloride (NaCl) induces changes in biofilm, mediated by increased production of polysaccharides intercellular adhesion (PIA). We identified 12 proteins that showed higher abundance in increased level of NaCl. This includes one important protein (IsaA) known to be associated with biofilm stability. In addition, we also found higher abundance of a cold shock protein, CspA, at higher NaCl. We have also identified several other proteins that are differentially expressed to the elevated levels of NaCl and mapped them in the regulatory pathways of PIA. The majority of proteins are involved with various aspects bacterial metabolic function. Our results demonstrated that NaCl influences gene regulatory networks controlling exopolysaccharide expression.
authors.affiliation: Department of Chemical and Biochemical Engineering, University of Maryland Baltimore County (UMBC), 1000 Hilltop Circle, Baltimore, MD 21250, USA.
authors.forename: Nazrul
authors.initials: N
authors.lastname: Islam
authors.affiliation: Department of Chemical and Biochemical Engineering, University of Maryland Baltimore County (UMBC), 1000 Hilltop Circle, Baltimore, MD 21250, USA.
authors.forename: Julia M
authors.initials: JM
authors.lastname: Ross
authors.affiliation: Department of Chemical and Biochemical Engineering, University of Maryland Baltimore County (UMBC), 1000 Hilltop Circle, Baltimore, MD 21250, USA.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Marten
keywords: Biofilm; Elevated; NaCl; Proteome; Staphylococcus aureus