title: Arabidopsis lipoxygenase 2 is essential for formation of green leaf volatiles and five-carbon volatiles.
abstract: Plants biosynthesize a variety of bioactive lipid derivatives, such as green leaf volatiles (GLVs) and jasmonates (JAs). Here we identify a lipoxygenase 2 (LOX2) involved in GLV biosynthesis in Arabidopsis using mutant lines for each of the six LOX isoforms present in Arabidopsis. We found that formation of five carbon volatiles was also dependent on LOX2. LOX2 is known to be involved in formation of JA; thus, LOX2 is apparently versatile in function. The results in this study suggested that LOX2 activity is suppressed in intact cells but activated upon tissue damage to support the rapid GLV-burst observed in wounded leaves.
authors.affiliation: Department of Applied Molecular Bioscience, Graduate School of Medicine and Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Mochizuki
authors.affiliation: Department of Applied Molecular Bioscience, Graduate School of Medicine and Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Japan.|MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI, USA.
authors.forename: Koichi
authors.initials: K
authors.lastname: Sugimoto
authors.affiliation: Department of Applied Molecular Bioscience, Graduate School of Medicine and Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Koeduka
authors.affiliation: Department of Applied Molecular Bioscience, Graduate School of Medicine and Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Japan.
authors.forename: Kenji
authors.identifier: http://orcid.org/0000-0002-4875-5176
authors.initials: K
authors.lastname: Matsui
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D020134:Catalytic Domain; D002851:Chromatography, High Pressure Liquid; D003080:Cold Temperature / Q000009:adverse effects; D003517:Cyclopentanes / Q000737:chemistry / Q000378:metabolism; D004789:Enzyme Activation; D008401:Gas Chromatography-Mass Spectrometry; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D008084:Lipoxygenase / Q000235:genetics / Q000378:metabolism*; D058945:Lipoxygenases / Q000235:genetics / Q000378:metabolism*; D015394:Molecular Structure; D016254:Mutagenesis, Insertional; D009154:Mutation; D054883:Oxylipins / Q000737:chemistry / Q000378:metabolism; D010802:Phylogeny; D018515:Plant Leaves / Q000201:enzymology* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D017354:Point Mutation; D052617:Solid Phase Microextraction; D055549:Volatile Organic Compounds / Q000737:chemistry / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D007527:Isoenzymes; D054883:Oxylipins; D055549:Volatile Organic Compounds; C011006:jasmonic acid; D058945:Lipoxygenases; C000610121:lipoxygenase 2, Arabidopsis; D008084:Lipoxygenase
keywords: Arabidopsis; green leaf volatiles; jasmonate; lipoxygenase