title: Chemical Composition, Cytotoxic and Antibacterial Activities of the Essential Oil from the Tunisian Ononis angustissima L. (Fabaceae).
abstract: The chemical composition, cytotoxic and antibacterial activities of the hydrodistilled essential oil of the aerial parts of Ononis angustissima from south Tunisia has been evaluated. The oil yield was 0.04% (w/w). The chemical composition, determined by GC and GC-MS is reported for the first time. Forty-five components, accounting for 93.7% of the total oil have been identified. The oil was characterized by a high proportion of oxygenated sesquiterpenes (33.2%), followed by sesquiterpene hydrocarbons (6.3%) and apocarotenoids (10.3%). The main components of the oil were α-eudesmol (22.4%), 2-tridecanone (9.3%) and acetophenone (7.4%). The essential oil was tested for its possible cytotoxic activity towards the human cervical cell line HeLa using the MTT assay and the antibacterial activity against Pseudomonas aeruginosa, Escherichia coli, Enterococcus faecalis, Staphylococcus aureus and the clinical strain Acinetobacter sp. This oil exerted a cytotoxic activity with an IC50 of 0.53 ± 0.02 mg/mL and a significant antibacterial effect against P. aeruginosa and E. faecalis.
authors.affiliation: Laboratoire de Chimie Hétérocyclique, Produit Naturels et Réactivité. Equipe : Chimie Médicinale et Produits Naturels, Département de Chimie, Faculté des Sciences de Monastir, Université de Monastir.
authors.forename: Lotfi
authors.initials: L
authors.lastname: Ghribi
authors.forename: Aymen
authors.initials: A
authors.lastname: Ben Nejma
authors.forename: Malek
authors.initials: M
authors.lastname: Besbes
authors.forename: Fethia
authors.initials: F
authors.lastname: Harzalla-Skhiri
authors.forename: Guido
authors.initials: G
authors.lastname: Flamini
authors.forename: Hichem
authors.initials: H
authors.lastname: Ben Jannet
mesh_terms: D002338:Carotenoids / Q000032:analysis; D002478:Cells, Cultured; D002849:Chromatography, Gas; D024881:Drug Resistance, Bacterial; D013293:Enterococcus faecalis / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects*; D007887:Fabaceae / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D009822:Oils, Volatile / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D010938:Plant Oils / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D012717:Sesquiterpenes / Q000032:analysis; D013211:Staphylococcus aureus / Q000187:drug effects; D014416:Tunisia
chemical_list: D009822:Oils, Volatile; D010938:Plant Oils; D012717:Sesquiterpenes; D002338:Carotenoids