title: Characterization and abiotic stress-responsive expression analysis of SGT1 genes in Brassica oleracea.
abstract: SGT1 genes are involved in enhancing plant responses to various biotic and abiotic stresses. Brassica oleracea is known to contain two types of SGT1 genes, namely suppressor of G2 allele of SKP1 and suppressor of GCR2. In this study, through systematic analysis, four putative SGT1 genes were identified and characterized in B. oleracea. In phylogenetic analysis, the genes clearly formed separate groups, namely BolSGT1a, BolSGT1b (both suppressor of G2 allele of SKP1 types), and BolSGT1 (suppressor of GCR2). Functional domain analysis and organ-specific expression patterns suggested possible roles for BolSGT1 genes during stress conditions. BolSGT1 genes showed significant changes in expression in response to heat, cold, drought, salt, or ABA treatment. Interaction network analysis supported the expression analysis, and showed that the BolSGT1a and BolSGT1b genes are strongly associated with co-regulators during stress conditions. However, the BolSGT1 gene did not show any strong association. Hence, BolSGT1 might be a stress resistance-related gene that functions without a co-regulator. Our results show that BolSGT1 genes are potential target genes to improve B. oleracea resistance to abiotic stresses such as heat, cold, and salt.
authors.affiliation: a Department of Horticulture, Sunchon National University, 413 Jungangno, Suncheon, Jeonnam 57922, Republic of Korea.
authors.forename: Ashokraj
authors.initials: A
authors.lastname: Shanmugam
authors.affiliation: a Department of Horticulture, Sunchon National University, 413 Jungangno, Suncheon, Jeonnam 57922, Republic of Korea.
authors.forename: Senthil Kumar
authors.initials: SK
authors.lastname: Thamilarasan
authors.affiliation: a Department of Horticulture, Sunchon National University, 413 Jungangno, Suncheon, Jeonnam 57922, Republic of Korea.
authors.forename: Jong-In
authors.initials: JI
authors.lastname: Park
authors.affiliation: b Department of Agricultural Education, Sunchon National University, 413 Jungangno, Suncheon, Jeonnam 57922, Republic of Korea.
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Jung
authors.affiliation: a Department of Horticulture, Sunchon National University, 413 Jungangno, Suncheon, Jeonnam 57922, Republic of Korea.
authors.forename: Ill-Sup
authors.initials: IS
authors.lastname: Nou
mesh_terms: D001937:Brassica / Q000235:genetics*; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D013312:Stress, Physiological / Q000235:genetics*; D013696:Temperature
chemical_list: D010940:Plant Proteins; D012965:Sodium Chloride
keywords: BolSGT1; Brassica oleracea var. capitata; abiotic stress resistance; expression génique; gene expression; résistance aux stress