title: Nonheterocytous cyanobacteria from Brazilian saline-alkaline lakes.
abstract: Saline-alkaline lakes are extreme environments that limit the establishment and development of life. The Nhecolândia, a subregion of the Pantanal wetland in Brazil, is characterized by the existence of ~500 saline-alkaline lakes, which support an underexplored and rich diversity of microorganisms. In this study, unicellular and homocytous cyanobacteria from five saline-alkaline lakes were accessed by culture-dependent approaches. Morphological evaluation and analyses of near complete sequences (~1400 nt) of the 16S rRNA genes were applied for phylogenetic and taxonomic placement. This polyphasic approach allowed for the determination of the taxonomic position of the isolated strains into the following genera: Cyanobacterium, Geminocystis, Phormidium, Leptolyngbya, Limnothrix, and Nodosilinea. In addition, fourteen Pseudanabaenales and Oscillatoriales representatives of putative novel taxa were found. These sequences fell into five new clades that could correspond to new generic units of the Pseudanabaenaceae and Phormidiaceae families.
authors.affiliation: Center for Nuclear Energy in Agriculture, University of São Paulo, Avenida Centenário 303, Piracicaba, 13400-970, São Paulo, Brazil.
authors.forename: Ana Paula Dini
authors.initials: AP
authors.lastname: Andreote
authors.affiliation: Center for Nuclear Energy in Agriculture, University of São Paulo, Avenida Centenário 303, Piracicaba, 13400-970, São Paulo, Brazil.
authors.forename: Marcelo Gomes Marçal Vieira
authors.initials: MG
authors.lastname: Vaz
authors.affiliation: Center for Nuclear Energy in Agriculture, University of São Paulo, Avenida Centenário 303, Piracicaba, 13400-970, São Paulo, Brazil.
authors.forename: Diego Bonaldo
authors.initials: DB
authors.lastname: Genuário
authors.affiliation: Center for Nuclear Energy in Agriculture, University of São Paulo, Avenida Centenário 303, Piracicaba, 13400-970, São Paulo, Brazil.|IRD, CNRS, UPS, OMP, Géoscience Environnement Toulouse, 19 Av. Edouard Belin, Toulouse, F-31400, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Barbiero
authors.affiliation: Department of Geography, Federal University of Mato Grosso do Sul, Rod. MS 134, Km 3, Nova Andradina, 79750-000, Mato Grosso do Sul, Brazil.
authors.forename: Ary Tavares
authors.initials: AT
authors.lastname: Rezende-Filho
authors.affiliation: Center for Nuclear Energy in Agriculture, University of São Paulo, Avenida Centenário 303, Piracicaba, 13400-970, São Paulo, Brazil.
authors.forename: Marli Fátima
authors.initials: MF
authors.lastname: Fiore
keywords: Brazil; Chroococcales; Oscillatoriales; Phylogeny; Pseudanabaenales; Synechococcales; alkaline environment; extreme environment; polyphasic approach; saline environment; tropical wetlands