title: Transcatheter Arterial Infusion of Autologous CD133(+) Cells for Diabetic Peripheral Artery Disease.
abstract: Microvascular lesion in diabetic peripheral arterial disease (PAD) still cannot be resolved by current surgical and interventional technique. Endothelial cells have the therapeutic potential to cure microvascular lesion. To evaluate the efficacy and immune-regulatory impact of intra-arterial infusion of autologous CD133(+) cells, we recruited 53 patients with diabetic PAD (27 of CD133(+) group and 26 of control group). CD133(+) cells enriched from patients' PB-MNCs were reinfused intra-arterially. The ulcer healing followed up till 18 months was 100% (3/3) in CD133(+) group and 60% (3/5) in control group. The amputation rate was 0 (0/27) in CD133(+) group and 11.54% (3/26) in control group. Compared with the control group, TcPO2 and ABI showed obvious improvement at 18 months and significant increasing VEGF and decreasing IL-6 level in the CD133(+) group within 4 weeks. A reducing trend of proangiogenesis and anti-inflammatory regulation function at 4 weeks after the cells infusion was also found. These results indicated that autologous CD133(+) cell treatment can effectively improve the perfusion of morbid limb and exert proangiogenesis and anti-inflammatory immune-regulatory impacts by paracrine on tissue microenvironment. The CD133(+) progenitor cell therapy may be repeated at a fixed interval according to cell life span and immune-regulatory function.
authors.affiliation: Department of Interventional and Vascular Surgery, Shanghai Tenth People's Hospital, Tongji University, No. 301 Middle Yan Chang Road, Shanghai 200072, China; Institution of Interventional and Vascular Surgery, Tongji University, No. 301 Middle Yan Chang Road, Shanghai 200072, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Interventional and Vascular Surgery, Shanghai Tenth People's Hospital, Tongji University, No. 301 Middle Yan Chang Road, Shanghai 200072, China.
authors.forename: Weishuai
authors.initials: W
authors.lastname: Lian
authors.affiliation: Department of Interventional Radiology, Nanjing First Hospital, No. 68 Changle Road, Nanjing, Jiangsu 210001, China.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Lou
authors.affiliation: Department of Interventional and Vascular Surgery, Shanghai Tenth People's Hospital, Tongji University, No. 301 Middle Yan Chang Road, Shanghai 200072, China.
authors.forename: Shilong
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Interventional and Vascular Surgery, Shanghai Tenth People's Hospital, Tongji University, No. 301 Middle Yan Chang Road, Shanghai 200072, China.
authors.forename: Chenhui
authors.initials: C
authors.lastname: Lu
authors.affiliation: Department of Interventional and Vascular Surgery, Shanghai Tenth People's Hospital, Tongji University, No. 301 Middle Yan Chang Road, Shanghai 200072, China.
authors.forename: Keqiang
authors.initials: K
authors.lastname: Zuo
authors.affiliation: Department of Interventional Radiology, Nanjing First Hospital, No. 68 Changle Road, Nanjing, Jiangsu 210001, China.
authors.forename: Haobo
authors.initials: H
authors.lastname: Su
authors.affiliation: Department of Interventional and Vascular Surgery, Shanghai Tenth People's Hospital, Tongji University, No. 301 Middle Yan Chang Road, Shanghai 200072, China.
authors.forename: Jichong
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Interventional and Vascular Surgery, Shanghai Tenth People's Hospital, Tongji University, No. 301 Middle Yan Chang Road, Shanghai 200072, China.
authors.forename: Chuanwu
authors.initials: C
authors.lastname: Cao
authors.affiliation: Department of Interventional and Vascular Surgery, Shanghai Tenth People's Hospital, Tongji University, No. 301 Middle Yan Chang Road, Shanghai 200072, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Tang
authors.affiliation: Department of Interventional and Vascular Surgery, Shanghai Tenth People's Hospital, Tongji University, No. 301 Middle Yan Chang Road, Shanghai 200072, China.
authors.forename: Zhongzhi
authors.initials: Z
authors.lastname: Jia
authors.affiliation: Department of Interventional and Vascular Surgery, Shanghai Tenth People's Hospital, Tongji University, No. 301 Middle Yan Chang Road, Shanghai 200072, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Jin
authors.affiliation: Unité de Recherche INSERM 602, 94807 Villejuif Cedex, France.
authors.forename: Georges
authors.initials: G
authors.lastname: Uzan
authors.affiliation: Department of Interventional Radiology, Nanjing First Hospital, No. 68 Changle Road, Nanjing, Jiangsu 210001, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Gu
authors.affiliation: Department of Interventional and Vascular Surgery, Shanghai Tenth People's Hospital, Tongji University, No. 301 Middle Yan Chang Road, Shanghai 200072, China; Institution of Interventional and Vascular Surgery, Tongji University, No. 301 Middle Yan Chang Road, Shanghai 200072, China.
authors.forename: Maoquan
authors.initials: M
authors.lastname: Li