title: Effects of Osseointegration by Bone Morphogenetic Protein-2 on Titanium Implants In Vitro and In Vivo.
abstract: This study designed a biomimetic implant for reducing healing time and achieving early osseointegration to create an active surface. Bone morphogenetic protein-2 (BMP-2) is a strong regulator protein in osteogenic pathways. Due to hardly maintaining BMP-2 biological function and specificity, BMP-2 efficient delivery on implant surfaces is the main challenge for the clinic application. In this study, a novel method for synthesizing functionalized silane film for superior modification with BMP-2 on titanium surfaces is proposed. Three groups were compared with and without BMP-2 on modified titanium surfaces in vitro and in vivo: mechanical grinding; electrochemical modification through potentiostatic anodization (ECH); and sandblasting, alkali heating, and etching (SMART). Cell tests indicated that the ECH and SMART groups with BMP-2 markedly promoted D1 cell activity and differentiation compared with the groups without BMP-2. Moreover, the SMART group with a BMP-2 surface markedly promoted early alkaline phosphatase expression in the D1 cells compared with the other surface groups. Compared with these groups in vivo, SMART silaning with BMP-2 showed superior bone quality and created contact areas between implant and surrounding bones. The SMART group with BMP-2 could promote cell mineralization in vitro and osseointegration in vivo, indicating potential clinical use.
authors.affiliation: Department of Dentistry, Kaohsiung Armed Forces General Hospital, Kaohsiung, Taiwan; School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Department of Prosthodontics, Kaohsiung Medical University Hospital, No. 100 Tzyou 1st Road, Kaohsiung 80708, Taiwan.
authors.forename: Fu-Yuan
authors.initials: FY
authors.lastname: Teng
authors.affiliation: Advanced Medical Devices and Composites Laboratory, Department of Fiber and Composite Materials, College of Engineering, Feng Chia University, Taichung, Taiwan.
authors.forename: Wen-Cheng
authors.initials: WC
authors.lastname: Chen
authors.affiliation: Department of Dentistry, Kaohsiung Armed Forces General Hospital, Kaohsiung, Taiwan.
authors.forename: Yin-Lai
authors.initials: YL
authors.lastname: Wang
authors.affiliation: School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Department of Prosthodontics, Kaohsiung Medical University Hospital, No. 100 Tzyou 1st Road, Kaohsiung 80708, Taiwan.
authors.forename: Chun-Cheng
authors.initials: CC
authors.lastname: Hung
authors.affiliation: Metal Industries Research & Development Centre, 3F, No. 88 Luke 5th Road, Luzhu District, Kaohsiung 82151, Taiwan.
authors.forename: Chun-Chieh
authors.initials: CC
authors.lastname: Tseng