title: Increase in mRNA Level of Orexin1 and 2 Receptors Following Induction of Experimental Autoimmune Encephalomyelitis in Mice.
abstract: Orexin A and B are hypothalamic peptides with a wide variety of effects such as anti-inflammation and neuroprotection. Impaired function of orexin system has been reported in some neurodegenerative diseases like Parkinson, Huntington and Alzheimer. In this study, the mRNA expression levels of some hypothalamic peptides were investigated in C57BL/6 female mice with experimental autoimmune encephalomyelitis (EAE). Animals were randomly divided into two control and EAE groups. EAE was induced by administration of myelin oligodendrocyte glycoprotein (MOG) with complete Ferund's adjuvant and pertussis toxin. Twenty-first days following immunization, mice were decapitated to remove the brains. Then, the expression profiles of prepro-orexin, orexin 1 receptors (OX1R) and orexin 2 receptors (OX2R) in hypothalamic region were assessed using real-time PCR method. In this study, we found a considerable increase in the mRNA expression of OX1R and OX2R following EAE induction in C57BL/6 mice. Elevation levels of OX1R and OX2R following EAE induction suggest that alteration in orexinergic system may involve in pathogenesis of multiple sclerosis.
authors.affiliation: Department of Pharmacology, School of Medicine and Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Iman
authors.initials: I
authors.lastname: Fatemi
authors.affiliation: Physiology-Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Shamsizadeh
authors.affiliation: Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Roohbakhsh
authors.affiliation: Geriatic Care Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Ayoobi
authors.affiliation: Department of Medical Genetics, National Institute for Genetic Engineering and Biotechnology, Tehran, Iran.
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Sanati
authors.affiliation: Department of Pharmacology, School of Medicine and Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Manijeh
authors.initials: M
authors.lastname: Motevalian
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism / Q000523:psychology; D005260:Female; D005620:Freund's Adjuvant; D007031:Hypothalamus / Q000378:metabolism; D008810:Mice, Inbred C57BL; D063308:Myelin-Oligodendrocyte Glycoprotein; D064446:Orexin Receptors / Q000235:genetics* / Q000378:metabolism; D037342:Pertussis Toxin; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation
chemical_list: C501103:Cd200r1 protein, mouse; C497459:Hcrtr1 protein, mouse; D063308:Myelin-Oligodendrocyte Glycoprotein; D064446:Orexin Receptors; D012333:RNA, Messenger; D005620:Freund's Adjuvant; D037342:Pertussis Toxin
keywords: C57BL/6 mice; Experimental autoimmune encephalomyelitis; Multiple sclerosis; Orexin 1 receptors; Orexin 2 receptors; Prepro-orexin