title: Soluble receptor and gene polymorphism for AGE: relationship with obesity and cardiovascular risks.
abstract: Obesity in adolescents has quadrupled in the past 30 y. Markers for cardiovascular risks are needed in this population. We hypothesized that soluble receptor for advanced glycation end products (sRAGE) and asymmetric dimethyl arginine (ADMA) can correlate with carotid intima-media thickness (cIMT), a known index of subclinical atherosclerosis. We also aimed to evaluate the frequency of (Gly82Ser) RAGE gene polymorphism in obese adolescents.
  
  
  
  Obese and nonobese adolescents were evaluated in a cross-sectional study for lipid profile, insulin resistance, ADMA, sRAGE, and RAGE gene (Gly 82 Ser) polymorphism. We measured cIMT in all subjects and performed correlation analyses with all markers.
  
  
  
  The study included 50 obese and 40 healthy control adolescents. Compared to controls, obese subjects had less sRAGE (P = 0.02) and greater cIMT (P = 0.006), insulin resistance (P < 0.0001), and ADMA (P < 0.0001). In a multivariate linear regression model, sRAGE was associated with cIMT (β = 0.28, P = 0.04). Both GS and SS genotypes of RAGE were more frequent in obese than controls (P = 0.04).
  
  
  
  Increased ADMA and decreased sRAGE are associated with cardiovascular risks in obese adolescents. The S allele in RAGE gene is more frequently detected with obesity. The role of RAGE gene and mechanisms leading to cardiovascular risks need further studying.
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Tanta University, Tanta, Egypt.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Rowisha
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Tanta University, Tanta, Egypt.
authors.forename: Manal
authors.initials: M
authors.lastname: El-Batch
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Tanta University, Tanta, Egypt.
authors.forename: Thanaa
authors.initials: T
authors.lastname: El Shikh
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Tanta University, Tanta, Egypt.
authors.forename: Salwa
authors.initials: S
authors.lastname: El Melegy
authors.affiliation: Department of Pediatrics, the George Washington University and Children's National Medical Center, Washington, DC.
authors.forename: Hany
authors.initials: H
authors.lastname: Aly
mesh_terms: D000293:Adolescent; D000483:Alleles; D001120:Arginine / Q000031:analogs & derivatives / Q000097:blood; D050197:Atherosclerosis / Q000097:blood; D015415:Biomarkers / Q000378:metabolism; D001794:Blood Pressure; D002318:Cardiovascular Diseases / Q000235:genetics*; D059168:Carotid Intima-Media Thickness; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D008055:Lipids; D008297:Male; D063766:Pediatric Obesity / Q000235:genetics*; D011110:Polymorphism, Genetic; D000067759:Receptor for Advanced Glycation End Products / Q000235:genetics*; D012044:Regression Analysis; D012307:Risk Factors
chemical_list: C000593871:AGER protein, human; D015415:Biomarkers; D008055:Lipids; D000067759:Receptor for Advanced Glycation End Products; C018524:N,N-dimethylarginine; D001120:Arginine