title: Quercetin ameliorates peripheral nerve ischemia-reperfusion injury through the NF-kappa B pathway.
abstract: This study aimed to investigate the protective effect of quercetin against ischemia-reperfusion (IR) injury induced in the sciatic nerve of the rat. Quercetin (20 mg/kg) was given during ischemia just before reperfusion. Four groups of rats (Q+IR3, Q+IR7, Q+IR14, and Q+IR28) received 3, 7, 14, and 28 days of reperfusion, respectively, after the intraperitoneal injection of quercetin. After reperfusion, a behavioral test was performed and the sciatic functional index was calculated. Each sciatic nerve was stained to check for edema and ischemic fiber degeneration. Immunohistochemical staining was performed to detect TNF-alpha and NF-kappa B, and TUNEL staining was carried out to detect apoptosis. The Q+IR3, Q+IR7, and Q+IR14 groups showed significantly increased behavioral scores and ameliorated sciatic functional index values compared to IR-injured rats that received vehicle alone during ischemia and then the same period of reperfusion. The Q+IR3, Q+IR7, Q+IR14, and Q+IR28 groups presented significant ischemic fiber degeneration (IFD), TNF-alpha expression, and apoptosis as compared with the IR-injured and perfused rats that did not receive quercetin. The Q+IR3, Q+IR7, and Q+IR28 groups also exhibited significantly decreased NF-kappa B expression (p < 0.001, p = 0.001, p = 0.026) as compared with the IR-injured rats that were perfused but did not receive quercetin. These results imply that quercetin may be beneficial in the treatment of sciatic IR injury because of its antiapoptotic and antiinflammatory effects and its ability to decrease the expression of NF-kappa B.
authors.affiliation: Razi Herbal Medicines Research Center and Department of Anatomy, Lorestan University of Medical Sciences, Khorramabad, Iran.
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Gholami
authors.affiliation: Razi Herbal Medicines Research Center and Department of Anatomy, Lorestan University of Medical Sciences, Khorramabad, Iran.
authors.forename: Zahra Khanipour
authors.initials: ZK
authors.lastname: Khayat
authors.affiliation: Department of Social Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
authors.forename: Khatereh
authors.initials: K
authors.lastname: Anbari
authors.affiliation: Razi Herbal Medicines Research Center and Department of Anatomy, Lorestan University of Medical Sciences, Khorramabad, Iran.
authors.forename: Zia
authors.initials: Z
authors.lastname: Obidavi
authors.affiliation: Department of Immunology, Lorestan University of Medical Sciences, Khorramabad, Iran.
authors.forename: Alimohammad
authors.initials: A
authors.lastname: Varzi
authors.affiliation: Razi Herbal Medicines Research Center and Department of Anatomy, Lorestan University of Medical Sciences, Khorramabad, Iran.
authors.forename: Mandana Beigi
authors.initials: MB
authors.lastname: Boroujeni
authors.affiliation: Department of Physiology and Pharmacology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Alipour
authors.affiliation: Research laboratory for embryology and stem cell, Department of Anatomical Sciences and pathology, Faculty of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Niapoor
authors.affiliation: Stem cells and tissue engineering research center, Shahroud University of Medical Sciences, Shahroud, Iran. annehgh@yahoo.com.
authors.forename: Anneh Mohammad
authors.initials: AM
authors.lastname: Gharravi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects; D008297:Male; D058990:Molecular Targeted Therapy; D016328:NF-kappa B / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D011794:Quercetin / Q000494:pharmacology* / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000235:genetics* / Q000473:pathology; D020426:Sciatic Neuropathy / Q000188:drug therapy* / Q000235:genetics* / Q000473:pathology; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology
chemical_list: D016328:NF-kappa B; D011794:Quercetin
keywords: Apoptosis; Ischemia–reperfusion; Ischemic fiber degeneration; NF-kappa B; Quercetin; Sciatic functional index; TNF-alpha