title: Proteomics for exploiting diversity of lupin seed storage proteins and their use as nutraceuticals for health and welfare.
abstract: Lupins have a variety of both traditional and modern uses. In the last decade, reports assessing the benefits of lupin seed proteins have proliferated and, nowadays, the pharmaceutical industry is interested in lupin proteins for human health. Modern genomics and proteomics have hugely contributed to describing the diversity of lupin storage genes and, above all, proteins. Most of these studies have been centered on few edible lupin species. However, Lupinus genus comprises hundreds of species spread throughout the Old and New Worlds, and these resources have been scarcely explored and exploited. We present here a detailed review of the literature on the potential of lupin seed proteins as nutraceuticals, and the use of -omic tools to analyze seed storage polypeptides in main edible lupins and their diversity at the Lupinus inter- and intra-species level. In this sense, proteomics, more than any other, has been a key approach. Proteomics has shown that lupin seed protein diversity, where post-translational modifications yield a large number of peptide variants with a potential concern in bioactivity, goes far beyond gene diversity. The future extended use of second and third generation proteomics should definitely help to go deeper into coverage and characterization of lupin seed proteome.
  
  
  
  Some important topics concerning storage proteins from lupin seeds are presented and analyzed in an integrated way in this review. Proteomic approaches have been essential in characterizing lupin seed protein diversity, which goes far beyond gene diversity since the protein level adds to the latter differential proteolytic cleavage of conglutin pro-proteins and a diverse array of glycosylation forms and sites. Proteomics has also proved helpful for screening and studying Lupinus germplasm with the future aim of exploiting and improving food production, quality, and nutritional values.
authors.affiliation: UMR CNRS 6553 Ecobio, OSUR (Observatoire des Sciences de l'Univers de Rennes), University of Rennes 1, 263 av. du Général Leclerc, 35042 Rennes, France. Electronic address: francisco.cabello@univ-rennes1.fr.
authors.forename: Francisco
authors.initials: F
authors.lastname: Cabello-Hurtado
authors.affiliation: UMR CNRS 6553 Ecobio, OSUR (Observatoire des Sciences de l'Univers de Rennes), University of Rennes 1, 263 av. du Général Leclerc, 35042 Rennes, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Keller
authors.affiliation: UMR CNRS 6553 Ecobio, OSUR (Observatoire des Sciences de l'Univers de Rennes), University of Rennes 1, 263 av. du Général Leclerc, 35042 Rennes, France.
authors.forename: José
authors.initials: J
authors.lastname: Ley
authors.affiliation: Agroforestry and Plant Biochemistry and Proteomics Research Group, Dpt. Biochemistry and Molecular Biology, University of Cordoba-CeiA3, Cordoba, Spain.
authors.forename: Rosa
authors.initials: R
authors.lastname: Sanchez-Lucas
authors.affiliation: Agroforestry and Plant Biochemistry and Proteomics Research Group, Dpt. Biochemistry and Molecular Biology, University of Cordoba-CeiA3, Cordoba, Spain.
authors.forename: Jesús V
authors.initials: JV
authors.lastname: Jorrín-Novo
authors.affiliation: UMR CNRS 6553 Ecobio, OSUR (Observatoire des Sciences de l'Univers de Rennes), University of Rennes 1, 263 av. du Général Leclerc, 35042 Rennes, France.
authors.forename: Abdelkader
authors.initials: A
authors.lastname: Aïnouche
mesh_terms: D019587:Dietary Supplements; D014644:Genetic Variation; D006801:Humans; D031297:Lupinus / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*; D055314:Seed Storage Proteins / Q000627:therapeutic use*
chemical_list: D055314:Seed Storage Proteins
keywords: Conglutins; Lupin proteome; Lupinus germplasm; Nutraceuticals; Seed proteome