title: Characterization and biological evaluation of the introduction of PLGA into biosilicate®.
abstract: The aims of this study were to characterize different BS/PLGA composites for their physicochemical and morphological characteristics and evaluate the in vitro and in vivo biological performance. The physicochemical and morphological modifications were analyzed by pH, mass loss, XRD, setting time, and SEM. For in vitro analysis, the osteoblast and fibroblast viability was evaluated. For in vivo evaluations, histopathology and immunohistochemistry were performed in a tibial defect in rats. After incubation, all composites presented lower values in pH and mass loss over time. Moreover, XRD and SEM analysis confirmed that the composites degraded over time. Additionally, pore formation was observed by SEM analysis after incubation mainly in BS/PLGA groups. BS/PLGA showed significantly increased in osteoblast viability 24 h. Moreover, BS/PLGA composites demonstrated an increase in fibroblast viability in all periods analyzed when compared to BS. In the in vivo study, after 2 and 6 weeks of implantation of biomaterials, histopathological findings revealed that the BS/PLGA composites degrades over time, mainly at periphery. Moreover, can be observed the presence of granulation tissue, bone formation, Runx-2, and RANKL immunoexpression in all groups. In conclusion, BS/PLGA composites present appropriate physicochemical characteristics, stimulate the cellular viability, and enhance the bone repair in vivo. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1063-1074, 2017.
authors.affiliation: Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, São Paulo, Brazil.
authors.forename: K R
authors.initials: KR
authors.lastname: Fernandes
authors.affiliation: Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, São Paulo, Brazil.
authors.forename: A M P
authors.initials: AMP
authors.lastname: Magri
authors.affiliation: Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, São Paulo, Brazil.
authors.forename: H W
authors.initials: HW
authors.lastname: Kido
authors.affiliation: Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, São Paulo, Brazil.
authors.forename: F
authors.initials: F
authors.lastname: Ueno
authors.affiliation: Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, São Paulo, Brazil.
authors.forename: L
authors.initials: L
authors.lastname: Assis
authors.affiliation: Department of Rehabilitation Sciences and Biophotonics Applied to Health Sciences, Nove de Julho University (UNINOVE), São Paulo, São Paulo, Brazil.
authors.forename: K P S
authors.initials: KPS
authors.lastname: Fernandes
authors.affiliation: Department of Rehabilitation Sciences and Biophotonics Applied to Health Sciences, Nove de Julho University (UNINOVE), São Paulo, São Paulo, Brazil.
authors.forename: R A
authors.initials: RA
authors.lastname: Mesquita-Ferrari
authors.affiliation: Institute of Chemistry, University of São Paulo (USP), São Carlos, São Paulo, Brazil.
authors.forename: V C
authors.initials: VC
authors.lastname: Martins
authors.affiliation: Institute of Chemistry, University of São Paulo (USP), São Carlos, São Paulo, Brazil.
authors.forename: A M
authors.initials: AM
authors.lastname: Plepis
authors.affiliation: Vitreous Materials Laboratory (LaMaV), Department of Materials Engineering, Federal University of São Carlos (UFSCar), Brazil.
authors.forename: E D
authors.initials: ED
authors.lastname: Zanotto
authors.affiliation: Vitreous Materials Laboratory (LaMaV), Department of Materials Engineering, Federal University of São Carlos (UFSCar), Brazil.
authors.forename: O
authors.initials: O
authors.lastname: Peitl
authors.affiliation: Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, São Paulo, Brazil.
authors.forename: D
authors.initials: D
authors.lastname: Ribeiro
authors.affiliation: Department of Biomaterials, Radboudumc, Nijmegen, The Netherlands.
authors.forename: J J
authors.initials: JJ
authors.lastname: van den Beucken
authors.affiliation: Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, São Paulo, Brazil.
authors.forename: A C M
authors.initials: ACM
authors.lastname: Renno
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000473:pathology; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology; D008422:Materials Testing; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism* / Q000473:pathology; D010012:Osteogenesis / Q000187:drug effects; D011100:Polyglycolic Acid / Q000737:chemistry / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017640:Silicates / Q000737:chemistry / Q000494:pharmacology; D013977:Tibia / Q000378:metabolism* / Q000473:pathology; D013978:Tibial Fractures / Q000378:metabolism / Q000473:pathology / Q000628:therapy*
chemical_list: D017640:Silicates; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: bioactive glass; biomaterials availability; bone; cytotoxicity; polymer