title: High incorporation of long-chain fatty acids contributes to the efficient production of acylated ghrelin in ghrelin-producing cells.
abstract: The mechanisms for supplying octanoic acid for ghrelin acylation in X/A-like cells are incompletely understood. We found that long-chain fatty acids were incorporated at a higher rate in the ghrelin-producing cell line MGN3-1 than in MIN6 cells, in part due to higher expression level of long-chain fatty acyl-CoA synthetase family member 1 (Acsl1). Inhibition of ACSLs by triacsin C profoundly suppressed acylated ghrelin production. These results suggest that high incorporation of long-chain fatty acids into the ghrelin-producing cells plays a role in the supply of octanoic acid for ghrelin acylation.
authors.affiliation: Medical Innovation Center, Kyoto University Graduate School of Medicine, Japan.
authors.forename: Mika
authors.initials: M
authors.lastname: Bando
authors.affiliation: Medical Innovation Center, Kyoto University Graduate School of Medicine, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Iwakura
authors.affiliation: Department of Diabetes, Endocrinology and Nutrition, Kyoto University Graduate School of Medicine, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Koyama
authors.affiliation: National Cerebral and Cardiovascular Center Research Institute, Osaka, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hosoda
authors.affiliation: Department of Health Science, Faculty of Medical Sciences, University of Fukui, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Shigematsu
authors.affiliation: The First Department of Medicine, Wakayama Medical University, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ariyasu
authors.affiliation: The First Department of Medicine, Wakayama Medical University, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Akamizu
authors.affiliation: Medical Innovation Center, Kyoto University Graduate School of Medicine, Japan.|National Cerebral and Cardiovascular Center Research Institute, Osaka, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kangawa
authors.affiliation: Medical Innovation Center, Kyoto University Graduate School of Medicine, Japan.
authors.forename: Kazuwa
authors.initials: K
authors.lastname: Nakao
mesh_terms: D000215:Acylation; D000217:Acyltransferases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002331:Carnitine / Q000031:analogs & derivatives / Q000378:metabolism; D002460:Cell Line; D003066:Coenzyme A Ligases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D019858:Enteroendocrine Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005230:Fatty Acids, Nonesterified / Q000737:chemistry / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D054439:Ghrelin / Q000378:metabolism*; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D054878:Lipoylation; D008565:Membrane Proteins; D008822:Mice, Transgenic; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D034622:RNA Interference; D011993:Recombinant Fusion Proteins; D014226:Triazenes / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D005230:Fatty Acids, Nonesterified; D054439:Ghrelin; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D014226:Triazenes; C116917:acylcarnitine; C034613:triacsin C; D000217:Acyltransferases; C526983:Mboat4 protein, mouse; C544400:ACSL1 protein, mouse; D003066:Coenzyme A Ligases; D002331:Carnitine
keywords: ghrelin; ghrelin O-acyltransferase; long-chain fatty acyl-CoA synthetase