title: Protective effects of Semiaquilegia adoxoides n-butanol extract against hydrogen peroxide-induced oxidative stress in human lens epithelial cells.
abstract: Context Hydrogen peroxide (H2O2)-induced damage in the lens epithelium leads to cell death and cataract. Semiaquilegia adoxoides (DC.) Makino (Ranunculaceae), a folk medicine of Hmong (an ethnic group of China), has been traditionally used to treat cataract; however, the underlying molecular mechanism is yet to be uncovered. Objective This study aimed to investigate whether the n-butanol extract of S. adoxoides (nSA) is effective against the H2O2-induced oxidative stress in human lens epithelial (HLE) cells. Materials and methods Human lens epithelial (SRA 01/04) cells were stimulated by H2O2 (250 μM) in the presence or absence of nSA. The antioxidant effects of nSA were determined in terms of cell viability (MTT assay), apoptosis (AnnexinV/PI staining), radical scavenging capability (various enzymatic assays), loss of mitochondrial membrane potential (Rhodamine 123 staining), expression of apoptotic markers including caspase-3 and caspase-9 and the change of Bcl-2/Bax ratio (western blot) in the HLE cells. Results The results showed that pretreatment of nSA (250, 500 and 1000 μg/mL) markedly reduced H2O2-induced cellular apoptosis and malondialdehyde accumulation, but elevated the activities of total superoxide dismutase, catalase, glutathione peroxidase. Thus, the total antioxidative capability was enhanced upon the nSA treatment meanwhile the loss of mitochondrial membrane potential was prevented. Moreover, nSA at concentrations of 250, 500 and 1000 μg/mL also significantly suppressed the activation of caspase-3 and -9, and increased the Bcl-2/Bax ratio in the HLE cells. Discussion and conclusion Our findings suggested that nSA is a potential prophylactic agent in the prevention of cataractogeneis.
authors.affiliation: a Department of Pharmacology, School of Basic Medical Science , Guiyang Medical University , Guiyang , China ;|b School of Pharmacy , Guiyang Medical University , Guiyang , China ;
authors.forename: Bing
authors.initials: B
authors.lastname: Liang
authors.affiliation: c School of Chinese Medicine , Hong Kong Baptist University , Kowloon Tong , Kowloon , Hong Kong.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: b School of Pharmacy , Guiyang Medical University , Guiyang , China ;
authors.forename: Jianta
authors.initials: J
authors.lastname: Wang
authors.affiliation: a Department of Pharmacology, School of Basic Medical Science , Guiyang Medical University , Guiyang , China ;
authors.forename: Mingming
authors.initials: M
authors.lastname: Zhang
authors.affiliation: b School of Pharmacy , Guiyang Medical University , Guiyang , China ;
authors.forename: Ran
authors.initials: R
authors.lastname: Xu
authors.affiliation: a Department of Pharmacology, School of Basic Medical Science , Guiyang Medical University , Guiyang , China ;
authors.forename: Fei
authors.initials: F
authors.lastname: Wu
authors.affiliation: b School of Pharmacy , Guiyang Medical University , Guiyang , China ;|c School of Chinese Medicine , Hong Kong Baptist University , Kowloon Tong , Kowloon , Hong Kong.
authors.forename: Haitao
authors.initials: H
authors.lastname: Xiao
authors.affiliation: b School of Pharmacy , Guiyang Medical University , Guiyang , China ;
authors.forename: Lei
authors.initials: L
authors.lastname: Tang
mesh_terms: D020001:1-Butanol / Q000737:chemistry*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D019610:Cytoprotection; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D007908:Lens, Crystalline / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D031948:Semiaquilegia / Q000737:chemistry; D012997:Solvents / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D051017:Apoptosis Regulatory Proteins; D010936:Plant Extracts; D012997:Solvents; D020001:1-Butanol; D006861:Hydrogen Peroxide
keywords: Apoptosis; Semiaquilegia adoxoides; cataract; human lens epithelial cells