title: Schwann-like cells differentiated from human dental pulp stem cells combined with a pulsed electromagnetic field can improve peripheral nerve regeneration.
abstract: The purpose of this study was to investigate the effect of Schwann-like cells combined with pulsed electromagnetic field (PEMF) on peripheral nerve regeneration. Schwann-like cells were derived from human dental pulp stem cells (hDPSCs) and verified with CD104, S100, glial fibrillary acidic protein (GFAP), laminin, and P75NTRimmunocytochemistry. Gene expression of P75NTRand S100 were analyzed. Male Sprague-Dawley rats (200-250g, 6-week-old) were divided into seven groups (n = 10 each): control, sham, PEMF, hDPSCs, hDPSCs + PEMF, Schwann-like cells, Schwann-like cells + PEMF. Cells were transplanted (1 × 106/10µl/rat) at crush-injury site or combined with PEMF (50 Hz, 1 h/day, 1 mT). Nerve regeneration was evaluated with functional test, histomorphometry and retrograde labelled neurons. Schwann-like cells expressed CD104, S100, GFAP, laminin, and p75 neurotrophin receptor (P75NTR). P75NTRand S100 mRNA expression was highest in Schwann-like cells + PEMF group, which also showed increased Difference and Gap scores. Axons and retrograde labeled neurons increased in all treatment groups. Schwann-like cells, hDPSCs with or without PEMF, and PEMF only improved peripheral nerve regeneration. Schwann-like cells + PEMF showed highest regeneration ability; PEMF has additive effect on hDPSCs, Schwann-like cell in vitro and nerve regeneration ability after transplantation in vivo. Bioelectromagnetics. 37:163-174, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Korea.|Key Laboratory of Oral Biomedical Engineering of Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: Hei
authors.affiliation: Graduate School of Bio and Information Technology, Hankyong National University, Anseong-si, Kyonggi-do, Seoul, Korea.
authors.forename: Soochan
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Oral Histology-Developmental Biology, School of Dentistry, Seoul National University, Seoul, Korea.
authors.forename: Joo-Cheol
authors.initials: JC
authors.lastname: Park
authors.affiliation: Dongguk Department of Medical Biotechnology, College of Life Science and Biotechnology, Dongguk University, Seoul, Korea.
authors.forename: Young-Kwon
authors.initials: YK
authors.lastname: Seo
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Korea.
authors.forename: Soung-Min
authors.initials: SM
authors.lastname: Kim
authors.affiliation: Dental Research Institute, Seoul National University, Seoul, Korea.
authors.forename: Jeong Won
authors.initials: JW
authors.lastname: Jahng
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Korea.|Dental Research Institute, Seoul National University, Seoul, Korea.
authors.forename: Jong-Ho
authors.initials: JH
authors.lastname: Lee
keywords: CD104; GFAP; P75NTR; S100; crush nerve injury