title: Fluorescence Immunoassay for Cocaine Detection.
abstract: A fluorescence immunoassay (FIA) has been developed for the detection of cocaine using norcocaine labeled with merocyanine dye and a monoclonal antibody specific to cocaine. Using this FIA, the detection range for cocaine was between 20.0 and 1700 μg/L with a limit of detection of 20.0 μg/L. Other cocaine derivatives did not interfere significantly with the detection when using this immunoassay technique with cross-reactivity values of less than 20%. Thus this FIA could be considered a useful tool for the detection of cocaine.
authors.affiliation: 1 R&D Division, Bioscience Technology Development Office, Panasonic Co., Ltd., Soraku-gun, Japan .|2 Graduate School of Science and Engineering, Kagoshima University , Kagoshima, Japan .
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakayama
authors.affiliation: 1 R&D Division, Bioscience Technology Development Office, Panasonic Co., Ltd., Soraku-gun, Japan .
authors.forename: Noriko
authors.initials: N
authors.lastname: Kenjjou
authors.affiliation: 1 R&D Division, Bioscience Technology Development Office, Panasonic Co., Ltd., Soraku-gun, Japan .
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Shigetoh
authors.affiliation: 2 Graduate School of Science and Engineering, Kagoshima University , Kagoshima, Japan .
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ito
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D001578:Benzopyrans / Q000737:chemistry / Q000276:immunology; D003042:Cocaine / Q000031:analogs & derivatives* / Q000276:immunology / Q000302:isolation & purification*; D005453:Fluorescence; D006801:Humans; D007118:Immunoassay; D007211:Indoles / Q000737:chemistry / Q000276:immunology; D057230:Limit of Detection
chemical_list: D000911:Antibodies, Monoclonal; D001578:Benzopyrans; D007211:Indoles; C548873:merocyanine; C008671:norcocaine; D003042:Cocaine