title: Tracing and inhibiting growth of Staphylococcus aureus in barbecue cheese production after product recall.
abstract: Staphylococcal food poisoning is one of the most prevalent causes of foodborne intoxication worldwide. It is caused by ingestion of enterotoxins formed by Staphylococcus aureus during growth in the food matrix. Following a recall of barbecue cheese due to the detection of staphylococcal enterotoxins in Switzerland in July 2015, we analyzed the production process of the respective dairy. Although most cheese-making processes involve acidification to inhibit the growth of pathogenic bacteria, barbecue cheese has to maintain a pH >6.0 to prevent undesired melting of the cheese. In addition, the dairy decided to retain the traditional manual production process of the barbecue cheese. In this study, therefore, we aimed to (1) trace Staph. aureus along the barbecue cheese production process, and (2) develop a sustainable strategy to inhibit growth of Staph. aureus and decrease the risk of staphylococcal food poisoning without changing the traditional production process. To this end, we traced Staph. aureus in a step-wise blinded process analysis on 4 different production days using spa (Staphylococcus protein A gene) typing, DNA microarray profiling, and pulsed-field gel electrophoresis analysis. We subsequently selected a new starter culture and used a model cheese production including a challenge test assay to assess its antagonistic effect on Staph. aureus growth, as well as its sensory and technological implications. We detected Staph. aureus in 30% (37/124) of the collected samples taken from the barbecue cheese production at the dairy. This included detection of Staph. aureus in the final product on all 4 production days, either after enrichment or using quantitative detection. We traced 2 enterotoxigenic Staph. aureus strains (t073/CC45 and t282/CC45) colonizing the nasal cavity and the forearms of the cheesemakers to the final product. In the challenge test assay, we were able to show that the new starter culture inhibited growth of Staph. aureus while meeting the sensory and technological requirements of barbecue cheese production.
authors.affiliation: Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, 8057 Zurich, Switzerland.
authors.forename: S
authors.initials: S
authors.lastname: Johler
authors.affiliation: Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, 8057 Zurich, Switzerland.
authors.forename: K
authors.initials: K
authors.lastname: Zurfluh
authors.affiliation: Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, 8057 Zurich, Switzerland. Electronic address: roger.stephan@uzh.ch.
authors.forename: R
authors.initials: R
authors.lastname: Stephan
mesh_terms: D000818:Animals; D002611:Cheese / Q000032:analysis / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D004768:Enterotoxins / Q000032:analysis; D005516:Food Microbiology / Q000379:methods*; D005542:Forearm / Q000382:microbiology; D005838:Genotype; D006801:Humans; D008892:Milk / Q000737:chemistry; D009296:Nasal Cavity / Q000382:microbiology; D020411:Oligonucleotide Array Sequence Analysis; D057051:Product Recalls and Withdrawals; D013202:Staphylococcal Food Poisoning / Q000382:microbiology / Q000517:prevention & control; D013205:Staphylococcal Protein A / Q000235:genetics; D013211:Staphylococcus aureus / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D013557:Switzerland
chemical_list: D004768:Enterotoxins; D013205:Staphylococcal Protein A
keywords: Staphylococcus aureus; barbecue cheese; contamination routes; process analysis; starter culture