title: Molecular insights on the effect of TGF-β1/-β3 in human corneal fibroblasts.
abstract: Transforming growth factor β (TGF-β) plays a critical role in wound healing and the pathogenesis of fibrosis (scarring). Three isoforms of TGF-β have been identified in mammals. Previous studies have shown that the addition of TGF-β1 (T1) or -β2 (T2) to human corneal fibroblasts (HCF) cultured in a 3-dimensional construct resulted in a fibrotic matrix, while the addition of TGF-β3 (T3) resulted in the production of enhanced non-fibrotic matrix as compared to control (Vitamin C [VitC] only). In the current investigation, we undertook the molecular comparison of fibrosis-related gene expression in T1 or T3-treated HCF to gain further insights into the regulation and roles of these two isoforms on the fibrotic response. HCF were cultured in 100 mm dishes in basic medium (Eagles minimum essential medium [EMEM] with 10% fetal bovine serum [FBS]). At 70-80% confluency, cells were exposed to basic medium with 0.5 mM 2-O-α-d-glucopyranosyl-l-ascorbic acid (VitC) ± 2 ng/ml of T1 or T3. After 4 h or 3 days, cells were harvested, and mRNA or protein was isolated. Fibrosis related mRNA levels were assayed using a commercial qRT-PCR Array. Selected proteins were examined using Western blotting (WB). Experiments were performed 6 times for the qRT-PCR and 4 times for WB for each condition. qRT-PCR results showed that most of the fibrosis-related genes were up or downregulated in HCF exposed to T1 or T3 as compared with VitC control. At 4 h, only Smad7 expression was significantly altered in T3-treated HCF, compared to T1, and at 3 days, five genes were altered. WB confirmed that T1 significantly decreased Smad7 expression compared to T3 and control, and that the expression of thrombospondin-1 in T3-stimulated HCF was enhanced compared to T1-treated cells. Finally, both T1 and T3 decreased Smad3 expression dramatically at both time points. At early time points, T1 and T3 have similar effects on expression of fibrosis related genes; however, with a longer exposure, an increasing number of genes were differentially expressed. Interestingly, most of the differentially expressed gene products are secreted by the cells and may be related to the modulation of extracellular matrix.
authors.affiliation: Schepens Eye Research Institute/MEE and Department of Ophthalmology, Harvard Medical School, Boston, MA, USA. Electronic address: Xiaoqing_Guo@meei.harvard.edu.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Guo
authors.affiliation: Schepens Eye Research Institute/MEE and Department of Ophthalmology, Harvard Medical School, Boston, MA, USA. Electronic address: Audrey_Hutcheon@meei.harvard.edu.
authors.forename: Audrey E K
authors.initials: AEK
authors.lastname: Hutcheon
authors.affiliation: Schepens Eye Research Institute/MEE and Department of Ophthalmology, Harvard Medical School, Boston, MA, USA. Electronic address: James_Zieske@meei.harvard.edu.
authors.forename: James D
authors.initials: JD
authors.lastname: Zieske
mesh_terms: D002478:Cells, Cultured; D003315:Cornea / Q000166:cytology / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D005136:Eye Proteins / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D005355:Fibrosis / Q000378:metabolism; D006801:Humans; D012333:RNA, Messenger / Q000378:metabolism; D051785:Smad Proteins / Q000378:metabolism; D019700:Thrombospondin 1 / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism / Q000502:physiology*; D053782:Transforming Growth Factor beta3 / Q000378:metabolism / Q000502:physiology*; D014945:Wound Healing / Q000502:physiology
chemical_list: D005136:Eye Proteins; D012333:RNA, Messenger; D051785:Smad Proteins; D019700:Thrombospondin 1; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D053782:Transforming Growth Factor beta3
keywords: Fibrosis; Gene array; Human corneal fibroblasts; Smad3; Smad7; TGF-beta1; TGF-beta3; Thrombospondin-1