title: Determination of Tetracycline Antibiotic Residues in Honey and Milk by Miniaturized Solid Phase Extraction Using Chitosan-Modified Graphitized Multiwalled Carbon Nanotubes.
abstract: A rapid, simple, and strongly selective miniaturized solid phase extraction (SPE) technique, requiring only small amounts of sorbent (24 mg) and elution solvent (600 μL), coupled with ultrahigh-performance liquid chromatography and quadrupole time-of-flight mass spectrometry was developed for detecting tetracycline antibiotics. These analytes were extracted from honey and milk using chitosan-modified graphitized multiwalled carbon nanotubes (G-MWNTs) as the solid sorbent and acetonitrile/acetic acid (8:2, v/v) as the eluent in miniaturized SPE. Under the optimum experimental conditions, a satisfactory linearity (r(2) > 0.992) was obtained, and the limits of detection were in the range of 0.61-10.34 μg/kg for the analytes. The mean recoveries of the five tetracycline antibiotic residues in the real samples were between 81.5 and 101.4%. The results demonstrated that chitosan-modified G-MWNTs comprise a promising material for the enrichment of tetracycline antibiotics from complex food matrices.
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University , Hangzhou 310036, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Xu
authors.affiliation: Department of Surgery, University of Michigan Medical Center , Ann Arbor, Michigan 48109, United States.
authors.forename: Mingrui
authors.initials: M
authors.lastname: An
authors.affiliation: Department of Surgery, University of Michigan Medical Center , Ann Arbor, Michigan 48109, United States.
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
authors.affiliation: Department of Surgery, University of Michigan Medical Center , Ann Arbor, Michigan 48109, United States.
authors.forename: Zhijing
authors.initials: Z
authors.lastname: Tan
authors.affiliation: Periodontics and Oral Medicine, School of Dentistry, University of Michigan , Ann Arbor, Michigan 48109, United States.
authors.forename: Jie
authors.initials: J
authors.lastname: Hao
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University , Hangzhou 310036, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University , Hangzhou 310036, China.
authors.forename: Li-Qing
authors.initials: LQ
authors.lastname: Peng
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University , Hangzhou 310036, China.
authors.forename: Wan
authors.initials: W
authors.lastname: Cao
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D048271:Chitosan / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D006722:Honey; D008892:Milk / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry; D013754:Tetracyclines / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D037742:Nanotubes, Carbon; D013754:Tetracyclines; D048271:Chitosan
keywords: chitosan; graphitized multiwalled carbon nanotubes; miniaturized solid phase extraction; quadrupole time-of-flight mass spectrometry; tetracycline antibiotics; ultrahigh-performance liquid chromatography