title: GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN.
abstract: OBJECTIVE
  : To evaluate and compare the behavior of glycosaminoglycans (GAGs) in Dupuytren disease (DD).
  
  
  METHODS
  : This is an experimental study with 23 patients diagnosed with DD. Tissue collected through fasciectomy with incision type Brunner or McCash were evaluated by electrophoresis for identification of GAGs. The quantification was carried out by immunofluorescence and dosage of proteins for different types of glycosaminoglycans. The results were expressed in percentage and statistically evaluated.
  
  
  RESULTS
  : A significant increase was observed through eletrophoresis in GAGs, as compared to the control (p<0.05). Immunofluorescence of hyaluronic acid was reduced (23 times) when compared to the control (p<0.0001).
  
  
  CONCLUSIONS
  : An increase of sulfated GAGs in Dupuytren's disease, mainly dermatan sulfate, was evident from our results, as well as a pronounced decrease of hyaluronic acid in the palmar aponeurosis from the same patients. Level of Evidence III, Case-Control Study.
authors.affiliation: . Universidade Federal de São Paulo (Unifesp), Escola Paulista de Medicina, Department of Orthopedics and Traumatology, São Paulo, SP, Brazil.
authors.forename: Priscilla Carneiro Hirai
authors.initials: PC
authors.lastname: Nascimento
authors.affiliation: . Universidade Federal de São Paulo (Unifesp), Escola Paulista de Medicina, Department of Orthopedics and Traumatology, São Paulo, SP, Brazil.
authors.forename: Elsa Yoko
authors.initials: EY
authors.lastname: Kobayashi
authors.affiliation: . Universidade Federal de São Paulo (Unifesp), Escola Paulista de Medicina, Department of Orthopedics and Traumatology, São Paulo, SP, Brazil.
authors.forename: Luiz Guilherme de Saboya
authors.initials: LG
authors.lastname: Lenzi
authors.affiliation: . Universidade Federal de São Paulo (Unifesp), Escola Paulista de Medicina, Department of Orthopedics and Traumatology, São Paulo, SP, Brazil.
authors.forename: João Baptista Gomes
authors.initials: JB
authors.lastname: Dos Santos
authors.affiliation: . Universidade Federal de São Paulo (Unifesp), Escola Paulista de Medicina, Department of Orthopedics and Traumatology, São Paulo, SP, Brazil.
authors.forename: Helena Bonciani
authors.initials: HB
authors.lastname: Nader
authors.affiliation: . Universidade Federal de São Paulo (Unifesp), Escola Paulista de Medicina, Department of Orthopedics and Traumatology, São Paulo, SP, Brazil.
authors.forename: Flávio
authors.initials: F
authors.lastname: Faloppa
keywords: Dupuytren contracture; Fascia; Glycosaminoglycans; Proteoglycans