title: Acetylpyrene-labelled 7-methylguanine nucleotides: unusual fluorescence properties and application to decapping scavenger activity monitoring.
abstract: 7-Methylguanosine (m(7)G) nucleotides labelled with acetylpyrene (AcPy) were synthesized as fluorescent mRNA 5' end (cap) analogues. The unique fluorescent properties of m(7)G-AcPy conjugates, different from G-AcPy, can be applied to studying various mRNA cap-related processes including the evaluation of putative inhibitors of DcpS enzyme-a therapeutic target in neuromuscular diseases.
authors.affiliation: Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, 02-089 Warsaw, Poland. asia@biogeo.uw.edu.pl.
authors.forename: Renata
authors.initials: R
authors.lastname: Kasprzyk
authors.forename: Joanna
authors.initials: J
authors.lastname: Kowalska
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Wieczorek
authors.forename: Mariusz
authors.initials: M
authors.lastname: Szabelski
authors.forename: Ryszard
authors.initials: R
authors.lastname: Stolarski
authors.forename: Jacek
authors.initials: J
authors.lastname: Jemielity
mesh_terms: D005453:Fluorescence; D006147:Guanine / Q000031:analogs & derivatives* / Q000737:chemistry; D009711:Nucleotides / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D009711:Nucleotides; D011721:Pyrenes; D006147:Guanine; C008450:7-methylguanine; C030984:pyrene