title: Dynamic-Electromembrane Extraction: A Technical Development for the Extraction of Neuropeptides.
abstract: In this work, a dynamic-electromembrane extraction (d-EME) device was developed for the extraction of neuropeptides. On the basis of a thin polypropylene hollow fiber (50 μm of wall-thickness and 280 μm i.d.), this setup allowed for a continual renewal of the acceptor compartment. Because of the reduced size of the device, high preconcentration factors were obtained (up to 50-fold). The extraction remained constant regardless of the extraction time (from 15 to 45 min); accordingly, this new setup minimized the effect of electrolysis on extraction performance while enabling high extraction yield (up to 72%) for most lipophilic neuropeptides.
authors.affiliation: School of Pharmaceutical Sciences, University of Geneva, University of Lausanne , Boulevard d'Yvoy 20, 1211 Geneva 4, Switzerland.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Drouin
authors.affiliation: School of Pharmaceutical Sciences, University of Geneva, University of Lausanne , Boulevard d'Yvoy 20, 1211 Geneva 4, Switzerland.
authors.forename: Serge
authors.initials: S
authors.lastname: Rudaz
authors.affiliation: School of Pharmaceutical Sciences, University of Geneva, University of Lausanne , Boulevard d'Yvoy 20, 1211 Geneva 4, Switzerland.
authors.forename: Julie
authors.initials: J
authors.lastname: Schappler
mesh_terms: D002851:Chromatography, High Pressure Liquid; D055664:Electrochemical Techniques; D006801:Humans; D059627:Liquid Phase Microextraction; D008567:Membranes, Artificial; D009479:Neuropeptides / Q000134:cerebrospinal fluid / Q000302:isolation & purification*; D011126:Polypropylenes / Q000737:chemistry; D053719:Tandem Mass Spectrometry
chemical_list: D008567:Membranes, Artificial; D009479:Neuropeptides; D011126:Polypropylenes