title: Blunted angiogenesis and hypertrophy are associated with increased fatigue resistance and unchanged aerobic capacity in old overloaded mouse muscle.
abstract: We hypothesize that the attenuated hypertrophic response in old mouse muscle is (1) partly due to a reduced capillarization and angiogenesis, which is (2) accompanied by a reduced oxidative capacity and fatigue resistance in old control and overloaded muscles, that (3) can be rescued by the antioxidant resveratrol. To investigate this, the hypertrophic response, capillarization, oxidative capacity, and fatigue resistance of m. plantaris were compared in 9- and 25-month-old non-treated and 25-month-old resveratrol-treated mice. Overload increased the local capillary-to-fiber ratio less in old (15 %) than in adult (59 %) muscle (P < 0.05). Although muscles of old mice had a higher succinate dehydrogenase (SDH) activity (P < 0.05) and a slower fiber type profile (P < 0.05), the isometric fatigue resistance was similar in 9- and 25-month-old mice. In both age groups, the fatigue resistance was increased to the same extent after overload (P < 0.01), without a significant change in SDH activity, but an increased capillary density (P < 0.05). Attenuated angiogenesis during overload may contribute to the attenuated hypertrophic response in old age. Neither was rescued by resveratrol supplementation. Changes in fatigue resistance with overload and aging were dissociated from changes in SDH activity, but paralleled those in capillarization. This suggests that capillarization plays a more important role in fatigue resistance than oxidative capacity.
authors.affiliation: School of Healthcare Science, Manchester Metropolitan University, Chester Street, John Dalton Building, Manchester, M1 5GD, UK.|Laboratory for Myology, Move Research Institute Amsterdam, Faculty of Behavioural and Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.
authors.forename: Sam B
authors.initials: SB
authors.lastname: Ballak
authors.affiliation: Laboratory for Myology, Move Research Institute Amsterdam, Faculty of Behavioural and Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.
authors.forename: Tinelies
authors.initials: T
authors.lastname: Busé-Pot
authors.affiliation: School of Healthcare Science, Manchester Metropolitan University, Chester Street, John Dalton Building, Manchester, M1 5GD, UK.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Harding
authors.affiliation: School of Healthcare Science, Manchester Metropolitan University, Chester Street, John Dalton Building, Manchester, M1 5GD, UK.
authors.forename: Moi H
authors.initials: MH
authors.lastname: Yap
authors.affiliation: Exercise Physiology Research Group, Department of Kinesiology, FaBeR, KU Leuven, Leuven, Belgium.
authors.forename: Louise
authors.initials: L
authors.lastname: Deldicque
authors.affiliation: School of Healthcare Science, Manchester Metropolitan University, Chester Street, John Dalton Building, Manchester, M1 5GD, UK.|Laboratory for Myology, Move Research Institute Amsterdam, Faculty of Behavioural and Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.
authors.forename: Arnold
authors.initials: A
authors.lastname: de Haan
authors.affiliation: Laboratory for Myology, Move Research Institute Amsterdam, Faculty of Behavioural and Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.
authors.forename: Richard T
authors.initials: RT
authors.lastname: Jaspers
authors.affiliation: School of Healthcare Science, Manchester Metropolitan University, Chester Street, John Dalton Building, Manchester, M1 5GD, UK. h.degens@mmu.ac.uk.|Lithuanian Sports University, Kaunas, Lithuania. h.degens@mmu.ac.uk.
authors.forename: Hans
authors.initials: H
authors.lastname: Degens
mesh_terms: D000375:Aging; D000818:Animals; D004195:Disease Models, Animal; D005221:Fatigue / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D006984:Hypertrophy; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018763:Muscle Fatigue; D018482:Muscle, Skeletal / Q000473:pathology / Q000503:physiopathology*; D009389:Neovascularization, Pathologic / Q000473:pathology*; D005082:Physical Exertion
keywords: Aerobic capacity; Angiogenesis; Hypertrophy; Muscle aging; Resveratrol