title: Nitric oxide mediates isoflavone accumulation and the antioxidant system enhancement in soybean sprouts.
abstract: In this study, we investigated the relationships between endogenous NO signal transduction pathways, the antioxidant system and isoflavone accumulation induced by UV-B radiation in soybean sprouts. Results showed that UV-B-triggered NO generation induced isoflavone accumulation by up-regulating the activity and gene expression of key enzymes (phenylalanine ammonia lyase, PAL; chalcone isomerase, CHI; chalcone synthase, CHS; isoflavone synthase, IFS) that participate in isoflavone biosynthesis and enhanced the antioxidant system by regulating levels of antioxidants (glutathione reductase, GR; glutathione S-transferase, GST; ascorbate peroxidase, APX; glutathione GSH; ascorbic acid, ASC), antioxidant enzyme activities (superoxide dismutase, SOD; peroxidase, POD; catalase, CAT) and their gene expression. These effects were inhibited by the addition of a specific NO-scavenger, carboxy-PTIO (cPTIO). The inhibition was reversed through application of the exogenous NO donor, SNP. Overall, NO is an essential signaling molecule, mediating UV-B-induced isoflavone accumulation and the antioxidant system enhancement in soybean sprouts.
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China.
authors.forename: Caifeng
authors.initials: C
authors.lastname: Jiao
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China.
authors.forename: Runqiang
authors.initials: R
authors.lastname: Yang
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China. Electronic address: guzx@njau.edu.cn.
authors.forename: Zhenxin
authors.initials: Z
authors.lastname: Gu
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D000975:Antioxidants / Q000737:chemistry*; D060387:Ascorbate Peroxidases / Q000235:genetics / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D001565:Benzoates / Q000737:chemistry; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005980:Glutathione Reductase / Q000235:genetics / Q000378:metabolism; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D007093:Imidazoles / Q000737:chemistry; D019753:Intramolecular Lyases / Q000235:genetics / Q000378:metabolism; D007529:Isoflavones / Q000737:chemistry*; D009569:Nitric Oxide / Q000737:chemistry*; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D014466:Ultraviolet Rays; D015854:Up-Regulation
chemical_list: D000975:Antioxidants; D001565:Benzoates; D007093:Imidazoles; D007529:Isoflavones; C079393:1,3-dihydroxy-4,4,5,5-tetramethyl-2-(4-carboxyphenyl)tetrahydroimidazole; D009569:Nitric Oxide; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D002374:Catalase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D000217:Acyltransferases; C020141:flavanone synthetase; D005982:Glutathione Transferase; D010650:Phenylalanine Ammonia-Lyase; D019753:Intramolecular Lyases; C019865:chalcone isomerase; D005978:Glutathione; D001205:Ascorbic Acid
keywords: Antioxidant system; Isoflavone; NO; Soybean sprouts; UV-B