title: Functional hydrophilic polystyrene beads with uniformly size and high cross-linking degree facilitated rapid separation of exenatide.
abstract: A high cross-linking polystyrene(PSt)-based anion-exchange material with uniformly size, high ion exchange capacity, and high hydrophilicity was synthesized by a novel surface functionalization approach in this study. Uniformly sized PSt microspheres were prepared by the membrane emulsion polymerization strategy, and then modified by (1) conversing resid ual surface vinyl groups to epoxy groups followed by quaternization, and (2) decorating aromatic ring matrix including nitration, reduction and attachment of glycidyltrimethylammonium chloride. The 3-D morphology and porous features of microspheres were observed by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The surface of the modified PSt became roughness but the particle size remained same. Meanwhile, FT-IR spectra and laser scanning confocal microscope (LCSM) indicated that the modification groups had been successfully covalently coated onto the PSt microspheres. Modified PSt microspheres showed greatly improved hydrophilicity and biocompatibility with 0.387mmol/mL ion exchange capacity (IEC). In the application evaluation procedure, exenatide can be purified from 42.9% (peptide crudes) to 88.6% by modified PSt column with 97.1% recovery yield. This modified PSt microspheres had a large potential in application for efficient separation of peptides.
authors.affiliation: National Key Lab of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: National Key Lab of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Chemical Engineering, Beijing Institute of Petro-chemical Technology, Beijing 102617, China.
authors.forename: Rongyue
authors.initials: R
authors.lastname: Zhang
authors.affiliation: National Key Lab of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: qiangli@home.ipe.ac.cn.
authors.forename: Yongdong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: National Key Lab of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: National Key Lab of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; Hebei Normal University of Science and Technology, Qinhuangdao, Hebei 066004, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.affiliation: National Key Lab of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Xuexing
authors.initials: X
authors.lastname: Wu
authors.affiliation: Hebei Normal University of Science and Technology, Qinhuangdao, Hebei 066004, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: National Key Lab of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Fangling
authors.initials: F
authors.lastname: Gong
authors.affiliation: National Key Lab of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Su
authors.affiliation: National Key Lab of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: ghma@ipe.ac.cn.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Ma
mesh_terms: D000077270:Exenatide; D057927:Hydrophobic and Hydrophilic Interactions; D013058:Mass Spectrometry; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D011137:Polystyrenes / Q000737:chemistry*; D014688:Venoms / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D010455:Peptides; D011137:Polystyrenes; D014688:Venoms; D000077270:Exenatide
keywords: Anion-exchange; Hydrophilicity; Peptide separation; Polystyrene microspheres