title: Practical implications of novel serum ELISA-assay for matrix metalloproteinase-8 in acute cardiac diagnostics.
abstract: Matrix metalloproteinases (MMPs) play a major role in inflammatory processes as they degrade extracellular proteins and modify immune responses. Inflammation is the driving factor in atherogenesis and MMPs, particularly MMP-8, has been linked to atherosclerotic plaque progression. MMP-8 is shown to be strongly associated with cardiovascular diseases (CVDs) and its complications thus providing a potential marker to identify patients at risk. Previously, laborious and expensive immunofluorometric assay (IFMA) was needed to reliably detect MMP-8 levels in serum. In this study, we compared a novel in-house ELISA-assay, dentoELISA, to the standard IFMA in determination of serum MMP-8 concentrations. As a cheaper and non-laborious assay, ELISA proved to be diagnostically as sensitive and specific as the IFMA. ROC statistics showed highly similar areas under the curve for both assays (0.779 versus 0.781). Furthermore, the concentrations measured by ELISA correlated significantly with concentrations determined with IFMA (r = 0.881, P < 0.001). In our study population, MMP-8 levels were significantly higher in the acute coronary syndrome patients (n = 2071) in comparison to reference population without significant coronary artery disease (n = 653). With this background, MMP-8-ELISA could provide interesting new approaches to novel CVD diagnostics.
authors.affiliation: a Department of Oral and Maxillofacial Diseases , University of Helsinki and Helsinki University Hospital , Helsinki , Finland.
authors.forename: Mikko T
authors.initials: MT
authors.lastname: Nieminen
authors.affiliation: b Heart and Lung Center HUH, University of Helsinki and Helsinki University Hospital , Helsinki , Finland.
authors.forename: Paula
authors.initials: P
authors.lastname: Vesterinen
authors.affiliation: a Department of Oral and Maxillofacial Diseases , University of Helsinki and Helsinki University Hospital , Helsinki , Finland.
authors.forename: Taina
authors.initials: T
authors.lastname: Tervahartiala
authors.affiliation: c Oral and Maxillofacial Department, University of Oulu and Oulu University Hospital , Oulu , Finland.
authors.forename: Immi
authors.initials: I
authors.lastname: Kormi
authors.affiliation: b Heart and Lung Center HUH, University of Helsinki and Helsinki University Hospital , Helsinki , Finland.
authors.forename: Juha
authors.initials: J
authors.lastname: Sinisalo
authors.affiliation: a Department of Oral and Maxillofacial Diseases , University of Helsinki and Helsinki University Hospital , Helsinki , Finland.
authors.forename: Pirkko J
authors.initials: PJ
authors.lastname: Pussinen
authors.affiliation: a Department of Oral and Maxillofacial Diseases , University of Helsinki and Helsinki University Hospital , Helsinki , Finland.|d Division of Periodontology, Department of Dental Medicine , Karolinska Institutet , Huddinge , Sweden.
authors.forename: Timo
authors.initials: T
authors.lastname: Sorsa
mesh_terms: D000208:Acute Disease; D015415:Biomarkers / Q000097:blood; D003324:Coronary Artery Disease / Q000175:diagnosis* / Q000201:enzymology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D020784:Matrix Metalloproteinase 8 / Q000097:blood*; D012372:ROC Curve
chemical_list: D015415:Biomarkers; D020784:Matrix Metalloproteinase 8
keywords: Coronary disease; ELISA; IFMA; inflammation; matrix metallo proteinases