title: Generation and characterization of the human iPSC line PBMC1-iPS4F1 from adult peripheral blood mononuclear cells.
abstract: Here we describe the generation and characterization of the human induced pluripotent stem cell (iPSC) line PBMC1-iPS4F1 from peripheral blood mononuclear cells from a healthy female with Spanish background. We used heat sensitive, non-integrative Sendai viruses containing the reprogramming factors Oct3/4, Sox2, Klf4 and c-Myc, whose expression was silenced in the established iPSC line. Characterization of the PBMC1-iPS4F1 cell line included analysis of typical pluripotency-associated factors at mRNA and protein level, alkaline phosphatase enzymatic activity, and in vivo and in vitro differentiation studies.
authors.affiliation: Gene Regulation, Stem Cells and Development Group, Department of Genomic Oncology, GENYO - Centre for Genomics and Oncological Research, Pfizer/University of Granada/Junta de Andalucía, PTS, Granada 18016, Spain. Electronic address: rmonteslorenzo@gmail.com.
authors.forename: Rosa
authors.initials: R
authors.lastname: Montes
authors.affiliation: Gene Regulation, Stem Cells and Development Group, Department of Genomic Oncology, GENYO - Centre for Genomics and Oncological Research, Pfizer/University of Granada/Junta de Andalucía, PTS, Granada 18016, Spain.
authors.forename: Tamara
authors.initials: T
authors.lastname: Romero
authors.affiliation: Otology & Neurotology Group CTS495, Department of Genomic Medicine, GENYO - Centre for Genomics and Oncological Research, Pfizer/University of Granada/Junta de Andalucía, PTS, Granada 18016, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Cabrera
authors.affiliation: Gene Regulation, Stem Cells and Development Group, Department of Genomic Oncology, GENYO - Centre for Genomics and Oncological Research, Pfizer/University of Granada/Junta de Andalucía, PTS, Granada 18016, Spain.
authors.forename: Veronica
authors.initials: V
authors.lastname: Ayllon
authors.affiliation: Otology & Neurotology Group CTS495, Department of Genomic Medicine, GENYO - Centre for Genomics and Oncological Research, Pfizer/University of Granada/Junta de Andalucía, PTS, Granada 18016, Spain; Department of Otolaryngology, University of Granada Hospital, Granada, Spain.
authors.forename: Jose A
authors.initials: JA
authors.lastname: Lopez-Escamez
authors.affiliation: Gene Regulation, Stem Cells and Development Group, Department of Genomic Oncology, GENYO - Centre for Genomics and Oncological Research, Pfizer/University of Granada/Junta de Andalucía, PTS, Granada 18016, Spain.
authors.forename: Veronica
authors.initials: V
authors.lastname: Ramos-Mejia
authors.affiliation: Gene Regulation, Stem Cells and Development Group, Department of Genomic Oncology, GENYO - Centre for Genomics and Oncological Research, Pfizer/University of Granada/Junta de Andalucía, PTS, Granada 18016, Spain.
authors.forename: Pedro J
authors.initials: PJ
authors.lastname: Real
mesh_terms: D000328:Adult; D002454:Cell Differentiation; D002460:Cell Line; D005260:Female; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D000090062:Kruppel-Like Factor 4; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*