title: Wewakazole B, a Cytotoxic Cyanobactin from the Cyanobacterium Moorea producens Collected in the Red Sea.
abstract: A mass spectrometry (MS)-guided isolation has led to the purification of a new cyanobactin, wewakazole B (1), along with the known compound curacin D from a Red Sea Moorea producens. The planar structure of 1 was elucidated using a combination of NMR and MS techniques. After ozonolysis and acid hydrolysis, the absolute configurations of the amino acid components of 1 were determined by chiral-phase LC-MS and HPLC analyses. Notably, compound 1 exhibited cytotoxic activity toward human MCF7 breast cancer cells (IC50 = 0.58 μM) and human H460 lung cancer cells (IC50 = 1.0 μM) and was also found to be inactive in a siderophore assay.
authors.forename: Julius Adam V
authors.initials: JA
authors.lastname: Lopez
authors.affiliation: Department of Marine Chemistry, Faculty of Marine Sciences, King Abdulaziz University , P.O. Box 80207, Jeddah 21589, Saudi Arabia.
authors.forename: Sultan S
authors.initials: SS
authors.lastname: Al-Lihaibi
authors.affiliation: Department of Marine Chemistry, Faculty of Marine Sciences, King Abdulaziz University , P.O. Box 80207, Jeddah 21589, Saudi Arabia.
authors.forename: Walied M
authors.initials: WM
authors.lastname: Alarif
authors.affiliation: Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University , P.O. Box 80260, Jeddah 21589, Saudi Arabia.|Department of Pharmacognosy, Faculty of Pharmacy, Minia University , Minia 61519, Egypt.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Abdel-Lateff
authors.affiliation: Environmental Science Research Laboratory, Central Research Institute of Electric Power Industry , Abiko 270-1194, Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Nogata
authors.forename: Kenji
authors.initials: K
authors.lastname: Washio
authors.forename: Masaaki
authors.initials: M
authors.lastname: Morikawa
authors.forename: Tatsufumi
authors.initials: T
authors.lastname: Okino
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D000458:Cyanobacteria / Q000737:chemistry*; D047630:Depsipeptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D019156:Indian Ocean; D008235:Lyngbya Toxins / Q000737:chemistry; D008386:Marine Biology; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010456:Peptides, Cyclic / Q000494:pharmacology*; D013844:Thiazoles / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D047630:Depsipeptides; D008235:Lyngbya Toxins; D010456:Peptides, Cyclic; D013844:Thiazoles; C473357:wewakazole; C116945:curacin D