title: Identification of a novel miR-146a from Pinctada martensii involved in the regulation of the inflammatory response.
abstract: Increasing evidence demonstrated that microRNAs (miRNAs) play critical roles in innate immunity in vertebrates and invertebrates. MiR-146a/b is reported as a key regulator of the immune response through mediating Toll-like receptor and cytokine signalling. In this study, a novel miR-146a was identified and characterised from Pinctada martensii (designated as pm-miR-146a), and its roles in modulating the inflammatory response after LPS stimulation were also investigated. Pm-miR-146a ubiquitously expressed in all examined tissues, with the highest level in the mantle and lowest expression in the haemolymph. Pm-miR-146a increased at 24 h after lipoplysaccharide injection, in union with up-regulated NF-κB (P < 0.05). The over-expression of pm-miR-146a in vivo could significantly inhibit the expression of macrophage migration inhibitory factor (MIF), the potential target gene predicted by miRanda, while enforcing pm-miR-146a involved in the down-regulation of NF-κB. Thus, we propose that pm-miR-146a plays a role of negative feedback regulation to the NF-κB signal by repressing the expression of the pro-inflammatory cytokine MIF. These findings revealed that miR-146a represents a critical role in inflammatory response and offers new evidence for miRNAs in the innate immunity of molluscs.
authors.affiliation: Fishery College, Guangdong Ocean University, Zhanjiang 524088, China; Pearl Breeding and Processing Engineering Technology Research Center of Guangdong Province, Zhanjiang 524088, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: Fishery College, Guangdong Ocean University, Zhanjiang 524088, China; Pearl Breeding and Processing Engineering Technology Research Center of Guangdong Province, Zhanjiang 524088, China.
authors.forename: Jinlian
authors.initials: J
authors.lastname: Liang
authors.affiliation: Fishery College, Guangdong Ocean University, Zhanjiang 524088, China; Pearl Breeding and Processing Engineering Technology Research Center of Guangdong Province, Zhanjiang 524088, China.
authors.forename: Ronglian
authors.initials: R
authors.lastname: Huang
authors.affiliation: Fishery College, Guangdong Ocean University, Zhanjiang 524088, China; Pearl Breeding and Processing Engineering Technology Research Center of Guangdong Province, Zhanjiang 524088, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Du
authors.affiliation: Fishery College, Guangdong Ocean University, Zhanjiang 524088, China; Pearl Breeding and Processing Engineering Technology Research Center of Guangdong Province, Zhanjiang 524088, China.
authors.forename: Qingheng
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Fishery College, Guangdong Ocean University, Zhanjiang 524088, China; Pearl Breeding and Processing Engineering Technology Research Center of Guangdong Province, Zhanjiang 524088, China.
authors.forename: Yuewen
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Fishery College, Guangdong Ocean University, Zhanjiang 524088, China; Pearl Breeding and Processing Engineering Technology Research Center of Guangdong Province, Zhanjiang 524088, China. Electronic address: jiaoyuhaida@163.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Jiao
mesh_terms: D000818:Animals; D015536:Down-Regulation; D007113:Immunity, Innate; D007249:Inflammation / Q000235:genetics; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D049911:Pinctada / Q000235:genetics* / Q000276:immunology*; D017433:Protein Structure, Secondary; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs; D016328:NF-kappa B
keywords: Inflammatory response; Lipopolysaccharide; Macrophage migration inhibitory factor; MiR-146a