title: A LC-MS-based workflow for measurement of branched fatty acid esters of hydroxy fatty acids.
abstract: Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are a recently discovered class of endogenous mammalian lipids with antidiabetic and anti-inflammatory effects. We previously identified 16 different FAHFA families, such as branched palmitic acid esters of hydroxy stearic acids (PAHSAs); each family consists of multiple isomers in which the branched ester is at different positions (e.g., 5- and 9-PAHSA). We anticipate increased need for PAHSA measurements as markers of metabolic and inflammatory health. In this protocol, we provide a detailed description of the extraction of FAHFAs from human or mouse tissues, their enrichment by solid-phase extraction and subsequent analysis by LC-MS. For a sample size of 6-12, the time frame is 2-3 d.
authors.affiliation: Salk Institute for Biological Studies, La Jolla, California, USA.
authors.forename: Tejia
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Salk Institute for Biological Studies, La Jolla, California, USA.
authors.forename: Shili
authors.initials: S
authors.lastname: Chen
authors.affiliation: Division of Endocrinology, Diabetes &Metabolism, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Ismail
authors.initials: I
authors.lastname: Syed
authors.affiliation: Wallenberg Laboratory, Department of Molecular and Clinical Medicine, University of Gothenburg, Gothenburg, Sweden.
authors.forename: Marcus
authors.initials: M
authors.lastname: Ståhlman
authors.affiliation: Salk Institute for Biological Studies, La Jolla, California, USA.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Kolar
authors.affiliation: Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA.
authors.forename: Edwin A
authors.initials: EA
authors.lastname: Homan
authors.affiliation: Salk Institute for Biological Studies, La Jolla, California, USA.
authors.forename: Qian
authors.initials: Q
authors.lastname: Chu
authors.affiliation: Lundberg Laboratory for Diabetes Research, Department of Molecular and Clinical Medicine, University of Gothenburg, Gothenburg, Sweden.
authors.forename: Ulf
authors.identifier: http://orcid.org/0000-0002-1439-4608
authors.initials: U
authors.lastname: Smith
authors.affiliation: Wallenberg Laboratory, Department of Molecular and Clinical Medicine, University of Gothenburg, Gothenburg, Sweden.
authors.forename: Jan
authors.initials: J
authors.lastname: Borén
authors.affiliation: Division of Endocrinology, Diabetes &Metabolism, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Barbara B
authors.initials: BB
authors.lastname: Kahn
authors.affiliation: Salk Institute for Biological Studies, La Jolla, California, USA.
authors.forename: Alan
authors.initials: A
authors.lastname: Saghatelian
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005227:Fatty Acids / Q000032:analysis* / Q000302:isolation & purification; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D013997:Time Factors; D057188:Workflow
chemical_list: D005227:Fatty Acids