title: Understanding hematopoiesis from a single-cell standpoint.
abstract: The cellular diversity of the hematopoietic system has been extensively studied, and a plethora of cell surface markers have been used to discriminate and prospectively purify different blood cell types. However, even within phenotypically identical fractions of hematopoietic stem and progenitor cells or lineage-restricted progenitors, significant functional heterogeneity is observed when single cells are analyzed. To address these challenges, researchers are now using techniques to follow single cells and their progeny to improve our understanding of the underlying functional heterogeneity. On November 19, 2015, Dr. David Kent and Dr. Leïla Perié, two emerging young group leaders, presented their recent efforts to dissect the functional properties of individual cells with a webinar series organized by the International Society for Experimental Hematology. Here, we provide a summary of the presented methods for cell labeling and clonal tracking and discuss how these different techniques have been employed to study hematopoiesis.
authors.affiliation: Cell Systems Dynamics Research Group, Department of Biosystems Science and Engineering, ETH Zürich, Basel, Switzerland. Electronic address: konstantinos.kokkaliaris@bsse.ethz.ch.
authors.forename: Konstantinos D
authors.initials: KD
authors.lastname: Kokkaliaris
authors.affiliation: Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI.
authors.forename: Daniel
authors.initials: D
authors.lastname: Lucas
authors.affiliation: Program of Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA.
authors.forename: Isabel
authors.initials: I
authors.lastname: Beerman
authors.affiliation: Department of Haematology, Wellcome Trust and MRC Cambridge Stem Cell Institute, Cambridge University, Cambridge, UK.
authors.forename: David G
authors.initials: DG
authors.lastname: Kent
authors.affiliation: Institut Curie, PSL Research University, Paris, France.
authors.forename: Leïla
authors.initials: L
authors.lastname: Perié
mesh_terms: D000818:Animals; D019070:Cell Lineage / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D055106:Genome-Wide Association Study; D023281:Genomics / Q000379:methods; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D059010:Single-Cell Analysis