title: Display of fungal hydrophobin on the Pichia pastoris cell surface and its influence on Candida antarctica lipase B.
abstract: To modify the Pichia pastoris cell surface, two classes of hydrophobins, SC3 from Schizophyllum commune and HFBI from Trichoderma reesei, were separately displayed on the cell wall. There was an observable increase in the hydrophobicity of recombinant strains. Candida antarctica lipase B (CALB) was then co-displayed on the modified cells, generating strains GS115/SC3-61/CALB-51 and GS115/HFBI-61/CALB-51. Interestingly, the hydrolytic and synthetic activities of strain GS115/HFBI-61/CALB-51 increased by 37 and 109 %, respectively, but decreased by 26 and 43 %, respectively, in strain GS115/SC3-61/CALB-51 compared with the hydrophobin-minus recombinant strain GS115/CALB-GCW51. The amount of glycerol by-product from the transesterification reaction adsorbed on the cell surface was significantly decreased following hydrophobin modification, removing the glycerol barrier and allowing substrates to access the active sites of lipases. Electron micrographs indicated that the cell wall structures of both recombinant strains appeared altered, including changes to the inner glucan layer and outer mannan layer. These results suggest that the display of hydrophobins can change the surface structure and hydrophobic properties of P. pastoris and affect the catalytic activities of CALB displayed on the surface of P. pastoris cells.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, People's Republic of China.
authors.forename: Pan
authors.initials: P
authors.lastname: Wang
authors.affiliation: Wadsworth Center, New York State Department of Health, Albany, NY, 12201, USA.
authors.forename: Jie
authors.initials: J
authors.lastname: He
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, People's Republic of China.|Department of Biological and Chemical Engineering, Guangxi University of Technology, Donghuan Rd., Liuzhou, 545006, People's Republic of China.
authors.forename: Yufei
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Wadsworth Center, New York State Department of Health, Albany, NY, 12201, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Reynolds
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, People's Republic of China.
authors.forename: Shuangyan
authors.initials: S
authors.lastname: Han
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, People's Republic of China.
authors.forename: Shuli
authors.initials: S
authors.lastname: Liang
authors.affiliation: Wadsworth Center, New York State Department of Health, Albany, NY, 12201, USA.|Department of Biomedical Sciences, School of Public Health, University at Albany, Albany, NY, 12201, USA.
authors.forename: Haixin
authors.initials: H
authors.lastname: Sui
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, People's Republic of China. feylin@scut.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lin
mesh_terms: D002175:Candida / Q000201:enzymology; D002473:Cell Wall / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D057927:Hydrophobic and Hydrophilic Interactions; D008049:Lipase / Q000235:genetics / Q000378:metabolism*; D010843:Pichia / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012567:Schizophyllum / Q000235:genetics / Q000378:metabolism*; D014242:Trichoderma / Q000235:genetics / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D008049:Lipase; C431349:lipase B, Candida antarctica
keywords: Candida antarctica lipase B; Co-display; Hydrophobicity; Hydrophobin; Pichia pastoris