title: Oral secretions from Mythimna separata insects specifically induce defence responses in maize as revealed by high-dimensional biological data.
abstract: Attack from insect herbivores poses a major threat to plant survival, and accordingly, plants have evolved sophisticated defence systems. Maize is cultivated as a staple crop worldwide, and insect feeding causes large production losses. Despite its importance in agriculture, little is known about how maize reacts to insect herbivory. Taking advantage of advances in sequencing and mass spectrometry technology, we studied the response of maize to mechanical wounding and simulated Mythimna separata (a specialist insect) herbivory by applying its oral secretions (OS) to wounds. In comparison to the responses induced by mechanical wounding, OS elicited larger and longer-lasting changes in the maize transcriptome, proteome, metabolome and phytohormones. Specifically, many genes, proteins and metabolites were uniquely induced or repressed by OS. Nearly 290 transcription factor genes from 39 families were involved in OS-induced responses, and among these, more transcription factor genes were specifically regulated by OS than by wounding. This study provides a large-scale omics dataset for understanding maize response to chewing insects and highlights the essential role of OS in plant-insect interactions.
authors.affiliation: Department of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Qi
authors.affiliation: Department of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
authors.forename: Guiling
authors.initials: G
authors.lastname: Sun
authors.affiliation: Department of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Department of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
authors.forename: Christian
authors.initials: C
authors.lastname: Hettenhausen
authors.affiliation: Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena 07745, Germany.|German Centre for Integrative Biodiversity Research (iDiv), Halle-Jena-Leipzig, Leipzig 04103, Germany.
authors.forename: Meredith C
authors.initials: MC
authors.lastname: Schuman
authors.affiliation: Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena 07745, Germany.
authors.forename: Ian T
authors.initials: IT
authors.lastname: Baldwin
authors.affiliation: Department of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Song
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Zhudong
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Guowang
authors.initials: G
authors.lastname: Xu
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Wu
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D060434:Herbivory; D009036:Moths / Q000502:physiology*; D010937:Plant Growth Regulators / Q000378:metabolism; D020543:Proteome; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome; D003313:Zea mays / Q000235:genetics / Q000378:metabolism*
chemical_list: D010937:Plant Growth Regulators; D020543:Proteome; D014157:Transcription Factors
keywords: metabolome; phytohormones; proteome; transcriptome