title: Profiling of microbial community of Odisha hot spring based on metagenomic sequencing.
abstract: Deulajhari hot spring has diverse temperature and pH range varying from 43 °C to 65 °C and 7.83 to 8.10 respectively. Dense foliage around Deulajhari hot spring contributes to the high total organic carbon content (TOC). In our experiment we took sediment samples from the two Deulajhari hot springs (S1 and S2) out of the cluster having temperature of 43 °C and 55 °C and pH of 7.83 and 7.14 respectively. Sediment samples were analysed using 16S rRNA of V3-V4 region by amplicon metagenome sequencing. Over 34 phyla were detected in cluster S1 and 32 phyla in cluster S2 at the existing physiochemical parameters temperature 43 °C, pH 7.83, electroconductivity 0.019 dSm(- 1), and total organic carbon (TOC) 3.80% for S1 and temperature 55 °C, pH 7.14, electroconductivity 0.019 dSm(- 1), and total organic carbon (TOC) 0.97% for S2. Existence of a vast number of unresolved sequences 179 out of 292 in S1 and 186 out of 314 in S2 at the genus level emphasizes the significance of our study. Metagenome sequence information for the both clusters S1 and S2 of Deulajhari is available at NCBI, SRA database with accession number SRX1459732 and SRX1459733 respectively. Direct link to the deposited data: www.ncbi.nlm.nih.gov/sra/SRX1459732 www.ncbi.nlm.nih.gov/sra/SRX1459733.
authors.affiliation: Centre of Biotechnology, Siksha 'O' Anushandhan University, Bhubaneswar, Odisha, India.
authors.forename: Archana
authors.initials: A
authors.lastname: Singh
authors.affiliation: Centre of Biotechnology, Siksha 'O' Anushandhan University, Bhubaneswar, Odisha, India.
authors.forename: Enketeswara
authors.initials: E
authors.lastname: Subudhi
keywords: 16S rRNA; Deulajhari; Illumina platform; Metagenome; TOC