title: Adapted Lethality: What We Can Learn from Guinea Pig-Adapted Ebola Virus Infection Model.
abstract: Establishment of small animal models of Ebola virus (EBOV) infection is important both for the study of genetic determinants involved in the complex pathology of EBOV disease and for the preliminary screening of antivirals, production of therapeutic heterologic immunoglobulins, and experimental vaccine development. Since the wild-type EBOV is avirulent in rodents, the adaptation series of passages in these animals are required for the virulence/lethality to emerge in these models. Here, we provide an overview of our several adaptation series in guinea pigs, which resulted in the establishment of guinea pig-adapted EBOV (GPA-EBOV) variants different in their characteristics, while uniformly lethal for the infected animals, and compare the virologic, genetic, pathomorphologic, and immunologic findings with those obtained in the adaptation experiments of the other research groups.
authors.affiliation: Department of Medicine, Novosibirsk State University, Pirogova Street 2, 630090 Novosibirsk-90, Russia; Institute of Internal and Preventive Medicine, Bogatkova Street 175/1, 630089 Novosibirsk-89, Russia.
authors.forename: S V
authors.initials: SV
authors.lastname: Cheresiz
authors.affiliation: Department of Medicine, Novosibirsk State University, Pirogova Street 2, 630090 Novosibirsk-90, Russia; Institute of Internal and Preventive Medicine, Bogatkova Street 175/1, 630089 Novosibirsk-89, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Semenova
authors.affiliation: Institute of Clinical Immunology, Yadrincevskaya Street 14, 630047 Novosibirsk-47, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Chepurnov