title: Label-free DNA biosensor based on resistance change of platinum nanoparticles assemblies.
abstract: A novel nanoparticle based biosensor for the fast and simple detection of DNA hybridization events is presented. The sensor utilizes hybridized DNA's charge transport properties, combining them with metallic nanoparticle networks that act as nano-gapped electrodes. The DNA hybridization events can be detected by a significant reduction in the sensor's resistance due to the conductive bridging offered by hybridized DNA. By modifying the nanoparticle surface coverage, which can be controlled experimentally being a function of deposition time, and the structural properties of the electrodes, an optimized biosensor for the in situ detection of DNA hybridization events is ultimately fabricated. The fabricated biosensor exhibits a wide response range, covering four orders of magnitude, a limit of detection of 1nM and can detect a single base pair mismatch between probe and complementary DNA.
authors.affiliation: Department of Applied Physics, National Technical University of Athens, Athens 15780, Greece. Electronic address: evskotad@central.ntua.gr.
authors.forename: Evangelos
authors.initials: E
authors.lastname: Skotadis
authors.affiliation: Department of Applied Physics, National Technical University of Athens, Athens 15780, Greece.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Voutyras
authors.affiliation: Department of Applied Physics, National Technical University of Athens, Athens 15780, Greece.
authors.forename: Marianneza
authors.initials: M
authors.lastname: Chatzipetrou
authors.affiliation: Biomedical Research Foundation of the Academy of Athens, Athens 11527, Greece.
authors.forename: Georgios
authors.initials: G
authors.lastname: Tsekenis
authors.affiliation: Department of Applied Physics, National Technical University of Athens, Athens 15780, Greece.
authors.forename: Lampros
authors.initials: L
authors.lastname: Patsiouras
authors.affiliation: Department of Applied Physics, National Technical University of Athens, Athens 15780, Greece.
authors.forename: Leonidas
authors.initials: L
authors.lastname: Madianos
authors.affiliation: Institute of Nanoscience and Nanotechnology NCSR Demokritos, Athens 60228, Greece.
authors.forename: Stavros
authors.initials: S
authors.lastname: Chatzandroulis
authors.affiliation: Department of Applied Physics, National Technical University of Athens, Athens 15780, Greece.
authors.forename: Ioanna
authors.initials: I
authors.lastname: Zergioti
authors.affiliation: Department of Applied Physics, National Technical University of Athens, Athens 15780, Greece. Electronic address: dtsouk@central.ntua.gr.
authors.forename: Dimitris
authors.initials: D
authors.lastname: Tsoukalas
mesh_terms: D020137:Base Pair Mismatch; D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation*; D004247:DNA / Q000032:analysis* / Q000235:genetics; D004553:Electric Conductivity; D055664:Electrochemical Techniques / Q000191:economics / Q000295:instrumentation; D004566:Electrodes; D004867:Equipment Design; D053768:Metal Nanoparticles / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D010984:Platinum / Q000737:chemistry*
chemical_list: D010984:Platinum; D004247:DNA
keywords: Biosensor; DNA; Electrochemical; Platinum nanoparticles; Resistance; Sputtering