title: Liquid Biopsy and its Potential for Management of Hepatocellular Carcinoma.
abstract: OBJECTIVE
  We summarized the recent findings of liquid biopsy in cancer field and discussed its potential utility in hepatocellular carcinoma.
  
  
  METHODS
  Literature published in MEDLINE, EMBASE, and Science Direct electronic databases was searched and reviewed.
  
  
  RESULTS
  Liquid biopsy specially referred to the detection of nucleic acids (circulating cell-free DNA, cfDNA) and circulating tumor cells (CTCs) in the blood of cancer patients. Compared to conventional single-site sampling or biopsy method, liquid biopsy had the advantages such as non-invasiveness, dynamic monitoring, and the most important of all, overcoming the limit of spatial and temporal heterogeneity. The genomic information of cancer could be profiled by genotyping cfDNA/CTC and subsequently applied to make molecular classification, targeted therapy guidance, and unveil drug resistance mechanisms. The serial sampling feature of liquid biopsy made it possible to monitor treatment response in a real-time manner and predict tumor metastasis/recurrence in advance.
  
  
  CONCLUSIONS
  Liquid biopsy is a non-invasive, dynamic, and informative sampling method with important clinical translational significance in cancer research and practice. Much work needs to be done before it is used in the management of HCC.
authors.affiliation: Liver Surgery Department, Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai, 20032, China. zhou.jian@zs-hospital.sh.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Liver Surgery Department, Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai, 20032, China.
authors.forename: Ao
authors.initials: A
authors.lastname: Huang
authors.affiliation: Liver Surgery Department, Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai, 20032, China.
authors.forename: Xin-Rong
authors.initials: XR
authors.lastname: Yang
mesh_terms: D001706:Biopsy / Q000379:methods*; D006528:Carcinoma, Hepatocellular / Q000628:therapy*; D004273:DNA, Neoplasm / Q000235:genetics*; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000628:therapy*; D008297:Male; D009360:Neoplastic Cells, Circulating / Q000473:pathology*
chemical_list: D004273:DNA, Neoplasm
keywords: Genetic aberrations; Hepatocellular carcinoma; Liquid biopsy; Next-generation sequencing