title: GATA4 negatively regulates bone sialoprotein expression in osteoblasts.
abstract: GATA4 has been reported to act as a negative regulator in osteoblast differentiation by inhibiting the Dlx5 transactivation of Runx2 via the attenuation of the binding ability of Dlx5 to the Runx2 promoter region. Here, we determine the role of GATA4 in the regulation of bone sialoprotein (Bsp) in osteoblasts. We observed that the overexpression of Runx2 or Sox9 induced the Bsp expression in osteoblastic cells. Silencing GATA4 further enhanced the Runx2- and Sox9-mediated Bsp promoter activity, whereas GATA4 overexpression down-regulated Bsp promoter activity mediated by Runx2 and Sox9. GATA4 also interacted with Runx2 and Sox9, by attenuating the binding ability of Runx2 and Sox9 to the Bsp promoter region. Our data suggest that GATA4 acts as a negative regulator of Bsp expression in osteoblasts. [BMB Reports 2016; 49(6): 343-348].
authors.affiliation: Shool of Biological Sciences, Seoul National University, Seoul 08826, Korea.
authors.forename: Insun
authors.initials: I
authors.lastname: Song
authors.affiliation: Departments of Pharmacology and Biomedical Sciences, Chonnam National University Medical School, Gwangju 61469, Korea.
authors.forename: Byung-Chul
authors.initials: BC
authors.lastname: Jeong
authors.affiliation: Department of Endocrinology, Ajou University, Suwon 16499, Korea.
authors.forename: Yong Jun
authors.initials: YJ
authors.lastname: Choi
authors.affiliation: Department of Endocrinology, Ajou University, Suwon 16499, Korea.
authors.forename: Yoon-Sok
authors.initials: YS
authors.lastname: Chung
authors.affiliation: Departments of Pharmacology and Biomedical Sciences, Chonnam National University Medical School, Gwangju 61469, Korea.
authors.forename: Nacksung
authors.initials: N
authors.lastname: Kim
mesh_terms: D000818:Animals; D002460:Cell Line; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D050991:GATA4 Transcription Factor / Q000378:metabolism*; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D018398:Homeodomain Proteins / Q000378:metabolism; D006801:Humans; D058574:Integrin-Binding Sialoprotein / Q000235:genetics* / Q000378:metabolism; D008810:Mice, Inbred C57BL; D010006:Osteoblasts / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D055758:SOX9 Transcription Factor / Q000378:metabolism
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; C501120:Dlx5 protein, mouse; D050991:GATA4 Transcription Factor; D018398:Homeodomain Proteins; D058574:Integrin-Binding Sialoprotein; D055758:SOX9 Transcription Factor