title: An ethanol extract of Ramulus mori improves blood circulation by inhibiting platelet aggregation.
abstract: Inappropriate platelet aggregation can cause blood coagulation and thrombosis. In this study, the effect of an ethanol extract of Ramulus mori (ERM) on blood circulation was investigated. The antithrombotic activity of ERM on rat carotid arterial thrombosis was evaluated in vivo, and the effect of ERM on platelet aggregation and blood coagulation time was evaluated ex vivo. To evaluate the safety of ERM, its cytotoxicity to platelets and its effect on tail bleeding time were assessed; ERM was not toxic to rat platelets and did not prolong bleeding time. Moreover, administering ERM to rats had a significant preventive effect on carotid arterial thrombosis in vivo, and significantly inhibited adenosine diphosphate- and collagen-induced platelet aggregation ex vivo, whereas it did not prolong coagulation periods, such as prothrombin time and activated partial thromboplastin time. The results suggest that ERM is effective in improving blood circulation via antiplatelet activity rather than anticoagulation activity.
authors.affiliation: a Departmentof Public Health Science (BK21 PLUS Program) , Graduate School, Korea University , Seoul , Republic of Korea.
authors.forename: Jiyun
authors.initials: J
authors.lastname: Lee
authors.affiliation: a Departmentof Public Health Science (BK21 PLUS Program) , Graduate School, Korea University , Seoul , Republic of Korea.
authors.forename: Gayeung
authors.initials: G
authors.lastname: Kwon
authors.affiliation: a Departmentof Public Health Science (BK21 PLUS Program) , Graduate School, Korea University , Seoul , Republic of Korea.
authors.forename: Jieun
authors.initials: J
authors.lastname: Park
authors.affiliation: b Department of Chemical Engineering and Biotechnology , Korea Polytechnic University , Shihung-si , Republic of Korea.
authors.forename: Jeong-Keun
authors.initials: JK
authors.lastname: Kim
authors.affiliation: c Department of Biology , Chungbuk National University , Chungbuk , Republic of Korea.
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Choe
authors.affiliation: c Department of Biology , Chungbuk National University , Chungbuk , Republic of Korea.
authors.forename: Yoonhee
authors.initials: Y
authors.lastname: Seo
authors.affiliation: a Departmentof Public Health Science (BK21 PLUS Program) , Graduate School, Korea University , Seoul , Republic of Korea.|d School of Biosystem and Biomedical Science, College of Health Science , Korea University , Seoul , Republic of Korea.
authors.forename: Young-Hee
authors.initials: YH
authors.lastname: Lim
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D002339:Carotid Arteries / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D003094:Collagen / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000431:Ethanol / Q000737:chemistry; D005343:Fibrinolytic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D031625:Morus / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D018547:Plant Stems / Q000737:chemistry; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013267:Stilbenes / Q000302:isolation & purification / Q000494:pharmacology; D013927:Thrombosis / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*
chemical_list: D005343:Fibrinolytic Agents; D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors; D013267:Stilbenes; D000431:Ethanol; C034912:puag-haad; D000244:Adenosine Diphosphate; D003094:Collagen
keywords: Ramulus mori; blood circulation; blood coagulation; oxyresveratrol; platelet aggregation