title: Isolation of Secondary Metabolites from the Soil-Derived Fungus Clonostachys rosea YRS-06, a Biological Control Agent, and Evaluation of Antibacterial Activity.
abstract: The fungus Clonostachys rosea is widely distributed all over the world. The destructive force of this fungus, as a biological control agent, is very strong to lots of plant pathogenic fungi. As part of the ongoing search for antibiotics from fungi obtained from soil samples, the secondary metabolites of C. rosea YRS-06 were investigated. Through efficient bioassay-guided isolation, three new bisorbicillinoids possessing open-ended cage structures, tetrahydrotrichodimer ether (1) and dihydrotrichodimer ether A and B (2 and 3), and 12 known compounds were obtained. Their structures were determined via extensive NMR, HR-ESI-MS, and CD spectroscopic analyses and X-ray diffraction data. Compounds 1-3 are rare bisorbicillinoids with a γ-pyrone moiety. The biological properties of 1-15 were evaluated against six different Gram-positive and Gram-negative bacteria. Bisorbicillinoids, 2-5, and TMC-151 C and E, 14 and 15, showed potent antibacterial activity.
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University , Lanzhou 730000, People's Republic of China.
authors.forename: Ming-Ming
authors.initials: MM
authors.lastname: Zhai
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University , Lanzhou 730000, People's Republic of China.
authors.forename: Feng-Ming
authors.initials: FM
authors.lastname: Qi
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University , Lanzhou 730000, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University , Lanzhou 730000, People's Republic of China.
authors.forename: Chun-Xiao
authors.initials: CX
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University , Lanzhou 730000, People's Republic of China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences , Lanzhou 730000, People's Republic of China.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Shi
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences , Lanzhou 730000, People's Republic of China.
authors.forename: Duo-Long
authors.initials: DL
authors.lastname: Di
authors.affiliation: Key Laboratory of Botanical Pesticide R&D in Shaanxi Province, Northwest A&F University , Yangling, Shaanxi 712100, People's Republic of China.
authors.forename: Ji-Wen
authors.initials: JW
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University , Lanzhou 730000, People's Republic of China.|Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences , Lanzhou 730000, People's Republic of China.
authors.forename: Quan-Xiang
authors.initials: QX
authors.lastname: Wu
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D061046:Biological Control Agents / Q000737:chemistry / Q000302:isolation & purification; D001952:Bridged-Ring Compounds; D002942:Circular Dichroism; D006999:Hypocreales / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D012987:Soil; D012988:Soil Microbiology; D021241:Spectrometry, Mass, Electrospray Ionization; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; D061046:Biological Control Agents; D001952:Bridged-Ring Compounds; D012987:Soil
keywords: Clonostachys rosea YRS-06; TMC-151; antibacterial activity; antifungal activity; dihydrotrichodimer ether; tetrahydrotrichodimer ether