title: Transcriptome profiling of Musculus longissimus dorsi in two cattle breeds with different intramuscular fat deposition.
abstract: Intramuscular fat (IMF) deposition is a physiological process in cattle and is highly variable among breeds suggesting a large influence of genetic factors besides environmental factors. In order to elucidate molecular pathways underlying the genetic variation in this trait we compared transcriptomes of Musculus longissimus dorsi (MLD) in steers of Japanese Black and Holstein Friesian cattle breeds fed a high energy diet typically applied in Japan to achieve maximum IMF content. We identified a total of 569 differentially expressed genes (DEGs) with the majority (433) up-regulated in Japanese Black cattle. This breed is characterized by an extreme capacity for IMF deposition. Subsequent Ingenuity Pathway Analysis (IPA) revealed a gene network linking parameters of cell morphology and maintenance with lipid metabolism. The data from this study were deposited in NCBI's Gene Expression Omnibus and are accessible through GEO Series accession number GSE75348. We provide here a dataset which is of potential value to dissect molecular pathways influencing differences in fat deposition under high-energy nutrition.
authors.affiliation: Institute for Muscle Biology and Growth, Leibniz Institute for Farm Animal Biology, D-18197 Dummerstorf, Germany.
authors.forename: Elke
authors.initials: E
authors.lastname: Albrecht
authors.affiliation: Institute for Muscle Biology and Growth, Leibniz Institute for Farm Animal Biology, D-18197 Dummerstorf, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Komolka
authors.affiliation: Institute for Genome Biology, Leibniz Institute for Farm Animal Biology, D-18197 Dummerstorf, Germany.
authors.forename: Siriluck
authors.initials: S
authors.lastname: Ponsuksili
authors.affiliation: Kuju Agricultural Research Center, Kyushu University, 878-0201 Kuju-cho, Oita, Japan.
authors.forename: Takafumi
authors.initials: T
authors.lastname: Gotoh
authors.affiliation: Institute for Genome Biology, Leibniz Institute for Farm Animal Biology, D-18197 Dummerstorf, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Wimmers
authors.affiliation: Institute for Muscle Biology and Growth, Leibniz Institute for Farm Animal Biology, D-18197 Dummerstorf, Germany.
authors.forename: Steffen
authors.initials: S
authors.lastname: Maak
keywords: Cattle; Holstein Friesian; Intramuscular fat; Japanese Black; Microarray; Musculus longissimus dorsi