title: [Study on Monosaccharide Compositions of Polysaccharide in Dendrobium Stems of Different Resources by PMP-HPCE].
abstract: OBJECTIVE
  To establish a PMP-HPCE method for comparing the monosaccharides of polysaccharide in tissue-cultured and wild Dedrobium huoshanese and Dedrobium moniliforme as well as wild Dedrobium henanese, in order to investigate the similarities of their bioactive components.
  
  
  METHODS
  The PMP-monosaccharides of polysaccharide from the five investigated Dedrobium samples were separated by HPCE on a fused silica capillary column(100 cm x 50 µm) at 25 °C with 350 mmol/L BAS (adjusted to pH 10 with 1.0 mol/L NaOH) as running buffer for 34 min. The applied voltage was 20 kV and the detection wavelength was set at 250 nm.
  
  
  RESULTS
  Total six monosaccharides including xylose, glucose, mannose, galactose, galacturonic acid and ribose were detected in the five Dendrobiurms samples and the similarity coefficients between the ten batches of the same Dendrobium species were all above 0. 98,while remarkable dissimilarity were exhibited among species and different resources.
  
  
  CONCLUSIONS
  PMP-HPCE technique combined with chemometrics is simple, convenient, precise, reproducible and proved to be an effective strategy for identifying the species and origins, especially in the quality assessment of Dendrobium stems.
authors.forename: Nai-dong
authors.initials: ND
authors.lastname: Chen
authors.forename: Yun-fei
authors.initials: YF
authors.lastname: Meng
authors.forename: Hou-jun
authors.initials: HJ
authors.lastname: Yao
authors.forename: Cai-yun
authors.initials: CY
authors.lastname: Cao
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Li
mesh_terms: D031667:Dendrobium / Q000737:chemistry* / Q000145:classification; D005690:Galactose; D005947:Glucose; D006603:Hexuronic Acids; D008358:Mannose; D009005:Monosaccharides / Q000737:chemistry*; D018547:Plant Stems / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry / Q000145:classification; D011134:Polysaccharides / Q000737:chemistry*; D012266:Ribose; D014994:Xylose
chemical_list: D006603:Hexuronic Acids; D009005:Monosaccharides; D011134:Polysaccharides; C007819:galacturonic acid; D012266:Ribose; D014994:Xylose; D005947:Glucose; D008358:Mannose; D005690:Galactose