title: Bacterial diversity assessment of pristine mangrove microbial community from Dhulibhashani, Sundarbans using 16S rRNA gene tag sequencing.
abstract: The global knowledge of microbial diversity and function in Sundarbans ecosystem is still scarce, despite global advancement in understanding the microbial diversity. In the present study, we have analyzed the diversity and distribution of bacteria in the tropical mangrove sediments of Sundarbans using 16S rRNA gene amplicon sequencing. Metagenome is comprised of 1,53,926 sequences with 108.8 Mbp data and with 55 ± 2% G + C content. Metagenome sequence data are available at NCBI under the Bioproject database with accession no. PRJNA245459. Bacterial community metagenome sequences were analyzed by MG-RAST software representing the presence of 56,547 species belonging to 44 different phyla. The taxonomic analysis revealed the dominance of phyla Proteobacteria within our dataset. Further taxonomic analysis revealed abundance of Bacteroidetes, Acidobactreia, Firmicutes, Actinobacteria, Nitrospirae, Cyanobacteria, Planctomycetes and Fusobacteria group as the predominant bacterial assemblages in this largely pristine mangrove habitat. The distribution of different community datasets obtained from four sediment samples originated from one sampling station at two different depths providing better understanding of the sediment bacterial diversity and its relationship to the ecosystem dynamics of this pristine mangrove sediment of Dhulibhashani in, Sundarbans.
authors.affiliation: Department of Biochemistry, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, West Bengal, India.
authors.forename: Pijush
authors.initials: P
authors.lastname: Basak
authors.affiliation: Department of Biochemistry, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, West Bengal, India.
authors.forename: Arnab
authors.initials: A
authors.lastname: Pramanik
authors.affiliation: Department of Biochemistry, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, West Bengal, India.
authors.forename: Sohan
authors.initials: S
authors.lastname: Sengupta
authors.affiliation: Department of Biochemistry, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, West Bengal, India.
authors.forename: Sudip
authors.initials: S
authors.lastname: Nag
authors.affiliation: Department of Biochemistry, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, West Bengal, India.
authors.forename: Anish
authors.initials: A
authors.lastname: Bhattacharyya
authors.affiliation: Department of Biochemistry, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, West Bengal, India.
authors.forename: Debojyoti
authors.initials: D
authors.lastname: Roy
authors.affiliation: Department of Biochemistry, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, West Bengal, India.
authors.forename: Rudradip
authors.initials: R
authors.lastname: Pattanayak
authors.affiliation: Department of Biochemistry, Bose Institute, P1/12, C. I. T. Road, Scheme VIIM, Kolkata 700054, West Bengal, India.
authors.forename: Abhrajyoti
authors.initials: A
authors.lastname: Ghosh
authors.affiliation: Amity University, Major Arterial Road, Action Area II, Kadampukur Village, Rajarhat, Newtown, Kolkata, India.
authors.forename: Dhrubajyoti
authors.initials: D
authors.lastname: Chattopadhyay
authors.affiliation: Department of Biochemistry, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, West Bengal, India.
authors.forename: Maitree
authors.initials: M
authors.lastname: Bhattacharyya
keywords: Mangrove sediment, Dhulibhashani, Metagenome, Pyrosequencing, Bacterial diversity