title: Selective fractionation of Sugar Beet Pulp for release of fermentation and chemical feedstocks; optimisation of thermo-chemical pre-treatment.
abstract: The effect of time and pressure on the selective extraction of sugar beet pectin using steam pre-treatment on unprocessed Sugar Beet Pulp was evaluated using a design of experiments approach. This process gave the highest solubilisation of pectin oligomers at a relatively low pressure and longer time (5Bar, 24min), whilst leaving the majority of the cellulose fraction intact. This method of steam pre-treatment fits into the concept of a sugar beet biorefinery as it valorises an existing waste stream without requiring any further physical processing such as milling or dilution with water. The residual cellulose fraction was enriched in cellulose and could be effectively fermented into ethanol by yeast after enzymatic digestion, producing 0.48g ethanol per gram of glucose.
authors.affiliation: Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK.
authors.forename: C
authors.initials: C
authors.lastname: Hamley-Bennett
authors.affiliation: The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, Gordon Street, London WC1H 0AH, UK.
authors.forename: G J
authors.initials: GJ
authors.lastname: Lye
authors.affiliation: Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK.
authors.forename: D J
authors.initials: DJ
authors.lastname: Leak
mesh_terms: D001089:Arabinose / Q000032:analysis; D027461:Beta vulgaris / Q000737:chemistry*; D019149:Bioreactors; D002241:Carbohydrates / Q000032:analysis; D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D006868:Hydrolysis; D008962:Models, Theoretical; D011506:Proteins / Q000302:isolation & purification; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D012995:Solubility; D013696:Temperature
chemical_list: D002241:Carbohydrates; D011506:Proteins; D000431:Ethanol; D001089:Arabinose
keywords: Biorefinery; Design of experiments; Ethanol fermentation; Steam explosion; Sugar Beet Pulp