title: Measuring Poliovirus Antigenicity by Surface Plasmon Resonance. Application for Potency Indicating Assays.
abstract: The D-antigen ELISA is the commonly accepted test for release of inactivated poliovirus containing vaccines. However, this test has a few drawbacks regarding the many variations in the method to quantify the D-unit. The result may depend on method and reagents used which makes standardization of inactivated polio vaccines, based on D-units, to a real challenge. This chapter describes a surface plasmon resonance based method to quantify D-units. The advantage of the calibrated D-antigen assay is the decrease in test variations because no labels, [no incubation times] and no washing steps are necessary. For standardization of both IPV and Sabin IPV, the calibration free concentration analysis could be an improvement as compared to ELISA or other SPR methods because this method combines quantity (particle concentration) and quality (antigenicity) in one assay.
authors.affiliation: Institute for Translational Vaccinology, P.O. Box 450, Antonie van Leeuwenhoeklaan 9, 3720 AL, Bilthoven, The Netherlands. janny.westdijk@intravacc.nl.
authors.forename: Janny
authors.initials: J
authors.lastname: Westdijk
authors.affiliation: Institute for Translational Vaccinology, P.O. Box 450, Antonie van Leeuwenhoeklaan 9, 3720 AL, Bilthoven, The Netherlands.
authors.forename: Larissa
authors.initials: L
authors.lastname: van der Maas
authors.affiliation: Institute for Translational Vaccinology, P.O. Box 450, Antonie van Leeuwenhoeklaan 9, 3720 AL, Bilthoven, The Netherlands.
authors.forename: Rimko
authors.initials: R
authors.lastname: Ten Have
authors.affiliation: Institute for Translational Vaccinology, P.O. Box 450, Antonie van Leeuwenhoeklaan 9, 3720 AL, Bilthoven, The Netherlands.
authors.forename: Gideon
authors.initials: G
authors.lastname: Kersten
mesh_terms: D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D000071497:Immunogenicity, Vaccine; D011051:Poliomyelitis / Q000276:immunology* / Q000517:prevention & control; D017955:Poliovirus / Q000276:immunology*; D011054:Poliovirus Vaccine, Inactivated / Q000276:immunology*; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D000956:Antigens, Viral; D011054:Poliovirus Vaccine, Inactivated
keywords: Calibrated D-ag assay; Calibration free concentration assay; D-antigen ELISA; IPV; Polio vaccine; Sabin; Surface plasmon resonance