title: ATF3 provides protection from Staphylococcus aureus and Listeria monocytogenes infections.
abstract: Activating transcription factor 3 (ATF3) is a stress-induced transcriptional regulator in eukaryote. The role of ATF3 in cancer has been well defined, but how ATF3 functions in bacterial infection is not well understood. Pneumococcal infection has been shown to induce ATF3 expression, which subsequently enhances cytokine production and provides protection from lethal Streptococcus pneumoniae infection, but the role of ATF3 in other Gram-positive (G(+)) infections remains unclear. Here, we report that infection with other G(+) bacteria (Staphylococcus aureus and Listeria monocytogenes) and with G(-) bacteria (uropathogenic Escherichia coli) also significantly induced ATF3 expression. Moreover, the production of cytokines (tumor necrosis factor alpha [TNF]-α, interleukin [IL]-1β, IL-6 and interferon [IFN]-γ) was enhanced by ATF3 in S. aureus and L. monocytogenes infection, but decreased in uropathogenic E. coli (UPEC) infection. In addition, in S. aureus and L. monocytogenes infections, ATF3 WT mice cleared bacteria more efficiently and had higher survival rates than ATF3 knockout mice. However, in UPEC infection, no significant difference was found in survival rate. Taken together, these data suggest that ATF3 provides protection from S. aureus and L. monocytogenes infections; however, the role of ATF3 in UPEC infection is more complicated and should be further elucidated.
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Su-Won 440-746, South Korea.
authors.forename: Cuong T
authors.initials: CT
authors.lastname: Nguyen
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Su-Won 440-746, South Korea.
authors.forename: Truc T
authors.initials: TT
authors.lastname: Luong
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Su-Won 440-746, South Korea.
authors.forename: Seungyeop
authors.initials: S
authors.lastname: Lee
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Su-Won 440-746, South Korea.
authors.forename: Gyu-Lee
authors.initials: GL
authors.lastname: Kim
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Su-Won 440-746, South Korea.
authors.forename: Suhkneung
authors.initials: S
authors.lastname: Pyo
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Su-Won 440-746, South Korea dkrhee@skku.ac.kr.
authors.forename: Dong-Kwon
authors.initials: DK
authors.lastname: Rhee
mesh_terms: D000072283:A549 Cells; D051700:Activating Transcription Factor 3 / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D004927:Escherichia coli Infections / Q000517:prevention & control*; D007371:Interferon-gamma / Q000096:biosynthesis; D053583:Interleukin-1beta / Q000096:biosynthesis; D008089:Listeria monocytogenes / Q000254:growth & development; D008088:Listeriosis / Q000517:prevention & control*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D013203:Staphylococcal Infections / Q000517:prevention & control*; D013211:Staphylococcus aureus / Q000254:growth & development; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D056689:Uropathogenic Escherichia coli / Q000254:growth & development
chemical_list: D051700:Activating Transcription Factor 3; C103002:Atf3 protein, mouse; C587672:IL1B protein, mouse; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma
keywords: Activating transcription factor 3 (ATF3); Listeria monocytogenes; Staphylococcus aureus; bacterial infection; cytokine