title: Hyperspectral imaging of plasmon resonances in metallic nanoparticles.
abstract: The spectroscopy of metal nanoparticles shows great potential for label-free sensing. In this article we present a hyper-spectral imaging system combined with a microfluidic system, which allows full spectroscopic characterization of many individual nanoparticles simultaneously (>50 particles). With such a system we were able overcome several limitations that are present in LSPR sensing with nanoparticle ensemble. We experimentally quantified (incorporating atomic force microscopy as well) the correlation between geometry, position of plasmon resonance (λPeak) and sensitivity of the particles (Sb=1.63λPeak-812.47[nm/RIU]). We were able to follow the adsorption of protein layers and determined their spatial inhomogeneity with the help of the hyperspectral imaging.
authors.affiliation: Leibniz Institute of Photonic Technology (IPHT) Jena, Albert-Einstein-Straße 9, 07745 Jena, Germany.
authors.forename: David
authors.initials: D
authors.lastname: Zopf
authors.affiliation: Leibniz Institute of Photonic Technology (IPHT) Jena, Albert-Einstein-Straße 9, 07745 Jena, Germany.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Jatschka
authors.affiliation: Leibniz Institute of Photonic Technology (IPHT) Jena, Albert-Einstein-Straße 9, 07745 Jena, Germany.
authors.forename: André
authors.initials: A
authors.lastname: Dathe
authors.affiliation: Leibniz Institute of Photonic Technology (IPHT) Jena, Albert-Einstein-Straße 9, 07745 Jena, Germany.
authors.forename: Norbert
authors.initials: N
authors.lastname: Jahr
authors.affiliation: Leibniz Institute of Photonic Technology (IPHT) Jena, Albert-Einstein-Straße 9, 07745 Jena, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Fritzsche
authors.affiliation: Leibniz Institute of Photonic Technology (IPHT) Jena, Albert-Einstein-Straße 9, 07745 Jena, Germany. Electronic address: ondrej.stranik@ipht-jena.de.
authors.forename: Ondrej
authors.initials: O
authors.lastname: Stranik
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D004867:Equipment Design; D005583:Fourier Analysis; D053768:Metal Nanoparticles / Q000032:analysis*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D012710:Serum Albumin, Bovine / Q000032:analysis; D013057:Spectrum Analysis / Q000295:instrumentation*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*
chemical_list: D012710:Serum Albumin, Bovine
keywords: Dark-field spectroscopy; Hyperspectral imaging; Plasmonic nanoparticle sensor; Single particle spectroscopy