title: Optimized Biotransformation of Icariin into Icariside II by β-Glucosidase from Trichoderma viride Using Central Composite Design Method.
abstract: A crude β-glucosidase has been produced from Trichoderma viride and used to explore a simple method to prepare icariside II from icariin. The crude enzyme has been studied by zymography method and used for hydrolysis of ICA. To achieve a high conversion rate of ICA, various factors have been studied including pH, reaction time, temperature, initial concentration of enzyme, and initial concentration of ICA through central composite design experiments. In the condition of the optimum hydrolysis parameters with pH 4.0, 41°C, 1.0 mg/mL ICA, and 9.8 U/mL crude β-glucosidase, the conversion rate of ICA reached 95.03% at 1 h. Moreover, the cytotoxicity test showed that ICA II performed inhibition effects on proliferation of A549 cell, while ICA has no cytotoxicity. It indicated that the hydrolysis transformation study of ICA is valuable for exploration of active new drugs.
authors.affiliation: Experiment Center of Teaching & Learning, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Cheng
authors.affiliation: Shanghai Xiangshan Hospital of Traditional Chinese Medicine, No. 528 in Middle of Renaissance Road, Shanghai 201203, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.affiliation: Experiment Center of Teaching & Learning, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Experiment Center of Teaching & Learning, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Yi-Shun
authors.initials: YS
authors.lastname: Yang
authors.affiliation: Experiment Center of Teaching & Learning, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; Department of Pharmaceutics, University of Washington, Seattle, WA 98105, USA.
authors.forename: Yue
authors.identifier: 0000-0002-7525-1221
authors.initials: Y
authors.lastname: Ding
mesh_terms: D001711:Biotransformation; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003603:Cytotoxins / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D005419:Flavonoids / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D013696:Temperature; D014242:Trichoderma / Q000201:enzymology; D001617:beta-Glucosidase / Q000737:chemistry*
chemical_list: D003603:Cytotoxins; D005419:Flavonoids; C060988:baohuoside I; D001617:beta-Glucosidase; C056599:icariin