title: Preparation of hybrid molecularly imprinted polymer with double-templates for rapid simultaneous purification of theophylline and chlorogenic acid in green tea.
abstract: A novel double-templates technique was adopted for solid-phase extraction packing agent, and the obtained hybrid molecularly imprinted polymers with double-templates (theophylline and chlorogenic acid) were characterized by fourier transform infrared and field emission scanning electron microscope. The molecular recognition ability and binding capability for theophylline and chlorogenic acid of polymers was evaluated by static absorption and dynamic adsorption curves. A rapid and accurate approach was established for simultaneous purification of theophylline and chlorogenic acid in green tea by coupling hybrid molecularly imprinted solid-phase extraction with high performance liquid chromatography. With optimization of SPE procedure, a reliable analytical method was developed for highly recognition towards theophylline and chlorogenic acid in green tea with satisfactory extraction recoveries (theophylline: 96.7% and chlorogenic acid: 95.8%). The limit of detection and limit of quantity of the method were 0.01 μg/mL and 0.03 μg/mL for theophylline, 0.05 μg/mL and 0.17 μg/mL for chlorogenic acid, respectively. The recoveries of proposed method at three spiked levels analysis were 98.7-100.8% and 98.3-100.2%, respectively, with the relative standard deviation less than 1.9%. Hybrid molecularly imprinted polymers with double-templates showed good performance for two kinds of targets, and the proposed approach with high affinity of hybrid molecularly imprinted polymers might offer a novel method for the purification of complex samples.
authors.affiliation: Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, School of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin 300384, PR China.
authors.forename: Weiyang
authors.initials: W
authors.lastname: Tang
authors.affiliation: Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, School of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin 300384, PR China.
authors.forename: Guizhen
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Chemistry and Chemical Engineering, Inha University, Incheon 402751, Republic of Korea.
authors.forename: Kyung Ho
authors.initials: KH
authors.lastname: Row
authors.affiliation: Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, School of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin 300384, PR China. Electronic address: zhutao@tjut.edu.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhu
mesh_terms: D000327:Adsorption; D002726:Chlorogenic Acid / Q000737:chemistry / Q000302:isolation & purification*; D054802:Molecular Imprinting; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*; D013662:Tea / Q000737:chemistry*; D013806:Theophylline / Q000737:chemistry / Q000302:isolation & purification*; D013997:Time Factors
chemical_list: D011108:Polymers; D013662:Tea; D002726:Chlorogenic Acid; D013806:Theophylline
keywords: Chlorogenic acid; Double-templates; Hybrid molecularly imprinted; Theophylline