title: Preliminary studies of new strains of Trametes sp. from Argentina for laccase production ability.
abstract: Oxidative enzymes secreted by white rot fungi can be applied in several technological processes within the paper industry, biofuel production and bioremediation. The discovery of native strains from the biodiverse Misiones (Argentina) forest can provide useful enzymes for biotechnological purposes. In this work, we evaluated the laccase and manganese peroxidase secretion abilities of four newly discovered strains of Trametes sp. that are native to Misiones. In addition, the copper response and optimal pH and temperature for laccase activity in culture supernatants were determined. The selected strains produced variable amounts of laccase and MnP; when Cu(2+) was added, both enzymes were significantly increased. Zymograms showed that two isoenzymes were increased in all strains in the presence of Cu(2+). Strain B showed the greatest response to Cu(2+) addition, whereas strain A was more stable at the optimal temperature and pH. Strain A showed interesting potential for future biotechnological approaches due to the superior thermo-stability of its secreted enzymes.
authors.affiliation: Laboratorio de Biotecnología Molecular, Módulo de Bioquímica y Farmacia, Facultad de Ciencias Exactas Químicas y Naturales, UNaM, Posadas, Misiones, Argentina. Electronic address: biotecmol2010@gmail.com.
authors.forename: María Isabel
authors.initials: MI
authors.lastname: Fonseca
authors.affiliation: Laboratorio de Biotecnología Molecular, Módulo de Bioquímica y Farmacia, Facultad de Ciencias Exactas Químicas y Naturales, UNaM, Posadas, Misiones, Argentina.
authors.forename: Marcos Raúl
authors.initials: MR
authors.lastname: Tejerina
authors.affiliation: Laboratorio de Biotecnología Molecular, Módulo de Bioquímica y Farmacia, Facultad de Ciencias Exactas Químicas y Naturales, UNaM, Posadas, Misiones, Argentina.
authors.forename: Silvana Soledad
authors.initials: SS
authors.lastname: Sawostjanik-Afanasiuk
authors.affiliation: Laboratorio de Biotecnología Molecular, Módulo de Bioquímica y Farmacia, Facultad de Ciencias Exactas Químicas y Naturales, UNaM, Posadas, Misiones, Argentina.
authors.forename: Ernesto Martin
authors.initials: EM
authors.lastname: Giorgio
authors.affiliation: Laboratorio de Biotecnología Molecular, Módulo de Bioquímica y Farmacia, Facultad de Ciencias Exactas Químicas y Naturales, UNaM, Posadas, Misiones, Argentina.
authors.forename: Mónica Lucrecia
authors.initials: ML
authors.lastname: Barchuk
authors.affiliation: Laboratorio de Biotecnología Molecular, Módulo de Bioquímica y Farmacia, Facultad de Ciencias Exactas Químicas y Naturales, UNaM, Posadas, Misiones, Argentina.
authors.forename: Pedro Darío
authors.initials: PD
authors.lastname: Zapata
authors.affiliation: Laboratorio de Biotecnología Molecular, Módulo de Bioquímica y Farmacia, Facultad de Ciencias Exactas Químicas y Naturales, UNaM, Posadas, Misiones, Argentina.
authors.forename: Laura Lidia
authors.initials: LL
authors.lastname: Villalba
mesh_terms: D001118:Argentina; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D042845:Laccase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013696:Temperature; D055454:Trametes / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D005656:Fungal Proteins; D042845:Laccase
keywords: Cu(2+) response; Laccase; Manganese peroxidase; Trametes; White rot fungi