title: MicroRNA-30c promotes natural killer cell cytotoxicity via up-regulating the expression level of NKG2D.
abstract: OBJECTIVE
  Natural killer (NK) cells play critical roles in antitumor immunity. Our previous study showed that over-expression of miR-30c-1* enhanced NKL cell cytotoxicity through up-regulation of tumor necrosis factor-α via directly targeting transcription factor homeobox containing 1. MiR-30c, the complimentary microRNA of miR-30c-1*, has been found to exert regulatory effect on T cell function. However, the effect of miR-30c on NK cells is unknown. Therefore, this study aimed to investigate whether miR-30c could play a role to enhance NK cell activation and cytotoxicity.
  
  
  METHODS
  Chemosynthesis exogenous miR-30c mimics and miR-30c inhibitor were transfected into NKL cells and isolated human peripheral blood NK cells, respectively. The expression levels of NK group 2, member D (NKG2D), CD107a and FasL on cell surface and cytotoxic ability of miRNAs transfected NKL cells against SMMC-7721 cells were evaluated.
  
  
  RESULTS
  MiR-30c could increase the expression of NKG2D and CD107a on NKL cells, and enhance cytotoxic ability of NKL cells to kill SMMC-7721 cells. Moreover, miR-30c could up-regulate the expression of FasL on both NKL cells and human peripheral blood NK cells. However, the peripheral blood NK cells from only four in ten healthy donors appeared high expression levels of NKG2D and CD107a after miR-30c transfection.
  
  
  CONCLUSIONS
  MiR-30c could promote the cytotoxicity of NKL cells in vitro by up-regulating the expression levels of NKG2D, CD107a and FasL. However, the effect of miR-30c on ex vivo NK cells from different human individuals is diverse, indicating that miR-30c may play complicate and fine adjustment in immune system.
authors.affiliation: Department of Immunology, the Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Immunology, the Fourth Military Medical University, Xi'an 710032, China; Hospital of Hubei Armed Police Corps, Wuhan, Hubei 430000, China.
authors.forename: Jiuyu
authors.initials: J
authors.lastname: Gong
authors.affiliation: Department of Immunology, the Fourth Military Medical University, Xi'an 710032, China; Department of Gynecology and Obstetrics, Tangdu Hospital, the Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Immunology, the Fourth Military Medical University, Xi'an 710032, China; Department of Gynecology and Obstetrics, Tangdu Hospital, the Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Wenwei
authors.initials: W
authors.lastname: Guo
authors.affiliation: Department of Immunology, the Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Boquan
authors.initials: B
authors.lastname: Jin
authors.affiliation: Department of Gynecology and Obstetrics, Tangdu Hospital, the Fourth Military Medical University, Xi'an 710038, China. Electronic address: xhwang_1968@126.com.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Immunology, the Fourth Military Medical University, Xi'an 710032, China. Electronic address: chenlh@fmmu.edu.cn.
authors.forename: Lihua
authors.initials: L
authors.lastname: Chen
mesh_terms: D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D053222:Fas Ligand Protein / Q000096:biosynthesis; D006801:Humans; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D052118:Lysosomal-Associated Membrane Protein 1 / Q000096:biosynthesis; D035683:MicroRNAs / Q000494:pharmacology*; D055655:NK Cell Lectin-Like Receptor Subfamily K / Q000096:biosynthesis*; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: C505661:FASLG protein, human; D053222:Fas Ligand Protein; C527085:KLRK1 protein, human; D052118:Lysosomal-Associated Membrane Protein 1; C546086:MIRN30b microRNA, human; D035683:MicroRNAs; D055655:NK Cell Lectin-Like Receptor Subfamily K
keywords: CD107a; Cytotoxicity; Fas ligand; MicroRNA-30c; NK group 2, member D (NKG2D); Natural killer cell