title: Simultaneous Determination and Pharmacokinetic Study of Protocatechuic Aldehyde and Its Major Active Metabolite Protocatechuic Acid in Rat Plasma by Liquid Chromatography-Tandem Mass Spectrometry.
abstract: A very simple and selective high-performance liquid chromatography electrospray ionization tandem mass spectrometry (LC-MS-MS) method was developed for simultaneous determination and pharmacokinetic study of protocatechuic aldehyde (PAL) and its active metabolite protocatechuic acid (PCA). The method involves a simple liquid-liquid extraction with ethyl acetate. The separation was performed on a Hypersil GOLD C18 column (2.1 × 150 mm, 3.0 µm; particle, Thermo, USA) with isocratic elution using a mobile phase consisted of methanol and water (containing 0.1% formic acid) at a flow rate of 0.2 mL/min. The detection of target compounds was done by using low-energy collision dissociation tandem mass spectrometry (CID-MS-MS) using the selective reaction monitoring scan mode. The method was linear for all analytes over the investigated range for all correlation coefficients greater than 0.9950. The lower limits of quantification were 2.0 ng/mL for PAL and PCA. The intra- and interday precisions (relative standard deviation, RSD %) were <6.84 and 5.54%, and the accuracy (relative error, RE %) was between -2.85 and 0.74% (n = 6). The developed method was applied to study the pharmacokinetics of PAL and its major active metabolite PCA in rat plasma after oral and intravenous administration of PAL.
authors.affiliation: Tasly Academy, Tianjin Tasly Group Co., Ltd., Tianjin, China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Tasly Academy, Tianjin Tasly Group Co., Ltd., Tianjin, China.
authors.forename: Kaijing
authors.initials: K
authors.lastname: Yan
authors.affiliation: Tasly Academy, Tianjin Tasly Group Co., Ltd., Tianjin, China School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Ma
authors.affiliation: Tasly Academy, Tianjin Tasly Group Co., Ltd., Tianjin, China Liwei_wzhy@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Tasly Academy, Tianjin Tasly Group Co., Ltd., Tianjin, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chu
authors.affiliation: Tasly Academy, Tianjin Tasly Group Co., Ltd., Tianjin, China.
authors.forename: Jiahua
authors.initials: J
authors.lastname: Guo
authors.affiliation: Tasly Academy, Tianjin Tasly Group Co., Ltd., Tianjin, China.
authors.forename: Shuming
authors.initials: S
authors.lastname: Li
authors.affiliation: Tasly Academy, Tianjin Tasly Group Co., Ltd., Tianjin, China.
authors.forename: Shuiping
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Tasly Academy, Tianjin Tasly Group Co., Ltd., Tianjin, China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Tianjin State Key Laboratory of Pharmacokinetics and Pharmacodynamics, Tianjin Institute of Pharmaceutical Research, Tianjin, China.
authors.forename: Changxiao
authors.initials: C
authors.lastname: Liu
mesh_terms: D000818:Animals; D001547:Benzaldehydes / Q000097:blood* / Q000493:pharmacokinetics; D002396:Catechols / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D062385:Hydroxybenzoates / Q000097:blood* / Q000493:pharmacokinetics; D057230:Limit of Detection; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D001547:Benzaldehydes; D002396:Catechols; D062385:Hydroxybenzoates; C009091:protocatechuic acid; C005581:protocatechualdehyde