title: Effects of immunological and hematological parameter in mice exposed to mixture of volatile organic compounds.
abstract: Exposure to some kinds of volatile organic compounds (VOCs) leads to immune system disorders, liver and kidney damage, hematological change. However, there is little information about the effect of VOCs mixture on immune system and hematological parameter. In this study, 50 Kunming male mice were exposed in five similar chambers, 0 (control) and four different doses of VOCs mixture (G1-4) for consecutively 10 days at 2 h/day. The concentrations of VOCs mixture were as follows: formaldehyde, benzene, toluene and xylene 1.0 + 1.1 + 2.0 + 2.0, 3.0 + 3.3 + 6.0 + 6.0, 5.0 + 5.5 + 10.0 + 10.0 and 10.0 + 11.0 + 20.0 + 20.0 mg/m(3), respectively, which corresponded to 10, 30, 50 and 100 times of indoor air quality standard in china. One day following VOCs exposure, spleen T lymphocyte subpopulation, serum biochemical markers and peripheral blood cells in mice were analyzed, respectively. VOCs exposure decreased significantly erythrocyte count (RBC), platelet (PLT) in peripheral blood in mice. While aspartate aminotransaminase (AST), alanine aminotransaminase (ALT), alkaline phosphatase (ALP) and creatinine (CREA) in serum increased significantly in G4 mice versus controls. Flow cytometry analysis showed that the number of splenic lymphocyte subpopulation cells decreased significantly in G2, 3 and 4 mice in comparison with normal Kunming mice. These results indicate inhalation of VOCs mixture affects CD4/8 subpopulations, liver, kidney function and some hematological parameters in mice.
authors.affiliation: a School of Biological Science, Luoyang Normal University , Luoyang , China .
authors.forename: Fan
authors.initials: F
authors.lastname: Wang
authors.affiliation: a School of Biological Science, Luoyang Normal University , Luoyang , China .
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: b School of Energy and Environment Engineering, Zhongyuan University of Technology , Zhengzhou , China , and.
authors.forename: Haifang
authors.initials: H
authors.lastname: Liu
authors.affiliation: a School of Biological Science, Luoyang Normal University , Luoyang , China .
authors.forename: Wanguang
authors.initials: W
authors.lastname: Chen
authors.affiliation: c College of Animal Science & Technology, Henan University of Science and Technology , Luoyang , China.
authors.forename: Xianli
authors.initials: X
authors.lastname: Si
authors.affiliation: c College of Animal Science & Technology, Henan University of Science and Technology , Luoyang , China.
authors.forename: Xiuying
authors.initials: X
authors.lastname: Ma
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D003404:Creatinine / Q000097:blood; D004906:Erythrocyte Count; D008297:Male; D051379:Mice; D009930:Organic Chemicals / Q000633:toxicity*; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
chemical_list: D000393:Air Pollutants; D009930:Organic Chemicals; D003404:Creatinine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase
keywords: Hematological parameters; VOCs; immunity function; lymphocyte subpopulation