title: Kinetic flux profiling dissects nitrogen utilization pathways in the oleaginous green alga Chlorella protothecoides.
abstract: As a promising candidate for biodiesel production, the green alga Chlorella protothecoides can efficiently produce oleaginous biomass and the lipid biosynthesis is greatly influenced by the availability of nitrogen source and corresponding nitrogen assimilation pathways. Based on isotope-assisted kinetic flux profiling (KFP), the fluxes through the nitrogen utilization pathway were quantitatively analyzed. We found that autotrophic C. protothecoides cells absorbed ammonium mainly through glutamate dehydrogenase (GDH), and partially through glutamine synthetase (GS), which was the rate-limiting enzyme of nitrogen assimilation process with rare metabolic activity of glutamine oxoglutarate aminotransferase (GOGAT, also known as glutamate synthase); whereas under heterotrophic conditions, the cells adapted to GS-GOGAT cycle for nitrogen assimilation in which GS reaction rate was associated with GOGAT activity. The fact that C. protothecoides chooses the adenosine triphosphate-free and less ammonium-affinity GDH pathway, or alternatively the energy-consuming GS-GOGAT cycle with high ammonium affinity for nitrogen assimilation, highlights the metabolic adaptability of C. protothecoides exposed to altered nitrogen conditions.
authors.affiliation: MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wu
authors.affiliation: Biosciences Center, National Renewable Energy Laboratory, Golden, Colorado, 80401, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Xiong
authors.affiliation: MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing, China.
authors.forename: Junbiao
authors.initials: J
authors.lastname: Dai
authors.affiliation: MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing, China.
authors.forename: Qingyu
authors.initials: Q
authors.lastname: Wu
mesh_terms: D000643:Ammonium Chloride / Q000737:chemistry / Q000378:metabolism; D052818:Autotrophic Processes; D002708:Chlorella / Q000254:growth & development* / Q000378:metabolism*; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D052836:Heterotrophic Processes; D007700:Kinetics; D053858:Metabolic Networks and Pathways; D008954:Models, Biological; D009584:Nitrogen / Q000378:metabolism*; D009587:Nitrogen Isotopes / Q000032:analysis / Q000378:metabolism
chemical_list: D009587:Nitrogen Isotopes; D000643:Ammonium Chloride; D005969:Glutamate Dehydrogenase; D005974:Glutamate-Ammonia Ligase; D009584:Nitrogen
keywords: Chlorella protothecoides; GDH pathway; GS-GOGAT cycle; KFP