title: Co-encapsulation of Nigella sativa oil and plasmid DNA for enhanced gene therapy of Alzheimer's disease.
abstract: Alzheimer disease involves genetic and non-genetic factors and hence it is rational to be treated with genetic and non-genetic therapeutic agents. Nigella sativa has multiple therapeutic properties including neuroregeneration. Nigella sativa oil (NSO) was encapsulated in PLGA nanoparticles and pDNA was loaded either by adsorption on chitosan-modified particles or encapsulation within PLGA nanoparticles. The particle size and zeta potential of NSO-pDNA-chitosan-PLGA nanoparticles were highly dependent on the medium and exhibited high burst release. Meanwhile, NSO-pDNA-PLGA nanoparticles were more consistent with lower burst release. The fabricated nanoparticles revealed the expected outcomes of both pDNA and NSO. The pDNA transfected N2a cell while the encapsulated NSO promoted neurite outgrowth that is crucial for neuroregeneration. Results from this study suggest that NSO could be added to the gene delivery carrier to enhance treatment benefits for Alzheimer disease.
authors.affiliation: a Department of Pharmaceutical Technology, Kulliyyah of Pharmacy , International Islamic University Malaysia , Bandar Indera Mahkota , Kuantan , Malaysia.
authors.forename: Abd Almonem
authors.initials: AA
authors.lastname: Doolaanea
authors.affiliation: a Department of Pharmaceutical Technology, Kulliyyah of Pharmacy , International Islamic University Malaysia , Bandar Indera Mahkota , Kuantan , Malaysia.
authors.forename: Nur 'Izzati
authors.initials: N'
authors.lastname: Mansor
authors.affiliation: a Department of Pharmaceutical Technology, Kulliyyah of Pharmacy , International Islamic University Malaysia , Bandar Indera Mahkota , Kuantan , Malaysia.
authors.forename: Nurul Hafizah
authors.initials: NH
authors.lastname: Mohd Nor
authors.affiliation: a Department of Pharmaceutical Technology, Kulliyyah of Pharmacy , International Islamic University Malaysia , Bandar Indera Mahkota , Kuantan , Malaysia.
authors.forename: Farahidah
authors.initials: F
authors.lastname: Mohamed
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics / Q000503:physiopathology / Q000628:therapy*; D000818:Animals; D002460:Cell Line; D004247:DNA / Q000008:administration & dosage* / Q000627:therapeutic use; D004337:Drug Carriers / Q000737:chemistry*; D015316:Genetic Therapy; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D055495:Neurogenesis / Q000187:drug effects; D010938:Plant Oils / Q000008:administration & dosage* / Q000627:therapeutic use; D010957:Plasmids / Q000008:administration & dosage / Q000627:therapeutic use; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D014162:Transfection / Q000379:methods; D029974:Carum
chemical_list: D004337:Drug Carriers; D010938:Plant Oils; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D004247:DNA; C104525:caraway oil
keywords: Alzheimer; Chitosan; Nigella sativa; PLGA; gene therapy; nanoparticle; plasmid DNA