title: Nanopuller-open data acquisition platform for AFM force spectroscopy experiments.
abstract: Atomic Force Microscope (AFM) is a widely used tool in force spectroscopy studies. Presently, this instrument is accessible from numerous vendors, albeit commercial solutions are expensive and almost always hardware and software closed. Approaches for open setups were published, as with modern low cost and readily available piezoelectric actuators, data acquisition interfaces and optoelectronic components building such force spectroscopy AFM is relatively easy. However, suitable software to control such laboratory made instrument was not released. Developing it in the lab requires significant time and effort. Our Nanopuller software described in this paper is intended to eliminate this obstacle. With only minimum adjustments this program can be used to control and acquire data with any suitable National Instruments universal digital/analog interface and piezoelectric actuator analog controller, giving significant freedom and flexibility in designing force spectroscopy experiment. Since the full code, written in a graphical LabVIEW environment is available, our Nanopuller can be easily customized. In this paper we describe the program and test its performance in controlling different setups. Successful and accurate force curve acquisition for standard samples (single molecules of I27O reference titin polyprotein and DNA as well as red blood cells) is shown.
authors.affiliation: Institute of Physics, Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University, Grudziadzka 5, 87-100 Torun, Poland.
authors.forename: Konrad
authors.initials: K
authors.lastname: Pawlak
authors.affiliation: Institute of Physics, Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University, Grudziadzka 5, 87-100 Torun, Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Strzelecki
mesh_terms: D064211:Connectin / Q000648:ultrastructure*; D004247:DNA / Q000648:ultrastructure*; D004912:Erythrocytes / Q000648:ultrastructure*; D006801:Humans; D018625:Microscopy, Atomic Force / Q000295:instrumentation / Q000379:methods*; D012984:Software; D014584:User-Computer Interface
chemical_list: D064211:Connectin; D004247:DNA
keywords: Atomic force microscope; DNA; Force spectroscopy; Nanoindentation; Proteins; Single molecule experiments