title: Survivin-targeted nanoparticles for pancreatic tumor imaging in mouse model.
abstract: We investigated the potential of targeting survivin, an inhibitor of apoptosis, in visualize pancreatic tumor in mouse model using targeted magnetic nanoparticles (MNPs) and magnetic resonance imaging (MRI). Chitosan-coated MNPS and survivin antisense oligonucleotide(ASON) were conjugated to give Sur-MNPs. Accumulations of targeted, non-targeted nanoparticles or nonsense oligonucleotide-MNPs (NSON-MNPs) in the liver, spleen, kidney and tumors were determined. Targeted nanoparticles were highly accumulated in BxPC-3 cells but not in non-cancer cells. In vivo MRI showed a significant T2 signal reduction in tumors of mice injected with targeted nanoparticles but slight signal change in tumors of mice injected with non-targeted nanoparticles or NSON-MNPs. Prussian blue staining demonstrated highly accumulated Sur-MNPs in tumor mass compared with normal pancreatic, kidney and liver tissues. Our data show that the MNPs functionalized with ASON lead to the targeted localization in pancreatic tumors. Survivin targeted nanoparticles could be used for detection of pancreatic tumors.
authors.affiliation: Department of Radiology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China. Electronic address: zhq2001us@163.com.
authors.forename: Zhongqiu
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Radiology, Suzhou Hospital, Nanjing Medical University, Suzhou, China.
authors.forename: Mingmin
authors.initials: M
authors.lastname: Tong
authors.affiliation: Department of Radiology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Oncology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China.
authors.forename: Shouyou
authors.initials: S
authors.lastname: Hu
authors.affiliation: Nanjing Origin Biosciences Inc., Nanjing, China.
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Bioelectronics, Jiangsu Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Radiology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Radiology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Yuefei
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Radiology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China.
authors.forename: Xiaoshuang
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Gastrointestinal Medical Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
authors.forename: Donghui
authors.initials: D
authors.lastname: Li
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D051034:Inhibitor of Apoptosis Proteins / Q000187:drug effects*; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000627:therapeutic use*; D051379:Mice; D010190:Pancreatic Neoplasms / Q000188:drug therapy*; D012097:Repressor Proteins / Q000187:drug effects*; D000077022:Survivin
chemical_list: C117749:Birc5 protein, mouse; D051034:Inhibitor of Apoptosis Proteins; D058185:Magnetite Nanoparticles; D012097:Repressor Proteins; D000077022:Survivin
keywords: Antisense oligonucleotide; Magnetic nanoparticles; Magnetic resonance imaging; Pancreatic cancer; Survivin