title: Antioxidant activity of olive wine, a byproduct of olive mill wastewater.
abstract: Context Although olive mill wastewater (OMWW) is a good source of bioactive phenolic compounds, disposing OMWW is a serious environmental challenge. Production of wine via fermenting OMWW may be a promising alternative to deal with OMWW. However, whether or not olive wine from OMWW still reserves its original bioactivities remains unclear. Objective This study examines antioxidant activity of olive wine fermented from OMWW. Materials and methods Hydroxytyrosol in olive oil was determined by HPLC. Total flavonoid, total polyphenol and in vitro antioxidant activities were measured by spectrophotometry. Aged mice were intragastricly administered 7, 14 and 28 mL/kg olive wine consecutively for 30 d. Afterward, levels of malonaldehyde (MDA), protein carbonyl, reduced glutathione (GSH) and activity of superoxide dismutase (SOD) were assayed in mouse plasma and liver. Results Contents of hydroxytyrosol, total flavonoid and total polyphenol in olive wine were 0.14 ± 0.01, 0.29 ± 0.06 and 0.43 ± 0.03 mg/mL, respectively. The IC50 value of olive wine to scavenge DPPH and hydroxyl free radicals was 2.5% and 3.2% (v/v), respectively. Compared with the solvent control group, olive wine with a dose of 28 mL/kg remarkably lowered mouse MDA concentration in liver, and reduced protein carbonyl level in plasma (p < 0.05). Meanwhile, olive wine at doses of 7 and 28 mL/kg notably enhanced SOD activity in both mouse plasma and liver (p < 0.05). The beneficial effect on liver was superior to that of γ-tocopherol. Conclusion The study demonstrated that olive wine from OMWW has potential for treating oxidative stress-associated diseases.
authors.affiliation: a Department of Key Laboratory of Medicinal and Edible Plant Resource Development of Sichuan Education , Chengdu University , Chengdu , China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yao
authors.affiliation: a Department of Key Laboratory of Medicinal and Edible Plant Resource Development of Sichuan Education , Chengdu University , Chengdu , China.
authors.forename: Gang
authors.initials: G
authors.lastname: He
authors.affiliation: a Department of Key Laboratory of Medicinal and Edible Plant Resource Development of Sichuan Education , Chengdu University , Chengdu , China.
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Guo
authors.affiliation: a Department of Key Laboratory of Medicinal and Edible Plant Resource Development of Sichuan Education , Chengdu University , Chengdu , China.
authors.forename: Yibing
authors.initials: Y
authors.lastname: Hu
authors.affiliation: a Department of Key Laboratory of Medicinal and Edible Plant Resource Development of Sichuan Education , Chengdu University , Chengdu , China.
authors.forename: Yuanfu
authors.initials: Y
authors.lastname: Shen
authors.affiliation: a Department of Key Laboratory of Medicinal and Edible Plant Resource Development of Sichuan Education , Chengdu University , Chengdu , China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Gou
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D015415:Biomarkers / Q000097:blood; D001713:Biphenyl Compounds / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D005260:Female; D005285:Fermentation; D005525:Food-Processing Industry; D005638:Fruit; D005978:Glutathione / Q000097:blood; D017665:Hydroxyl Radical / Q000737:chemistry; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D031658:Olea / Q000737:chemistry; D018384:Oxidative Stress / Q000187:drug effects*; D010626:Phenylethyl Alcohol / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D010946:Plants, Medicinal; D050050:Protein Carbonylation; D013482:Superoxide Dismutase / Q000097:blood; D062065:Wastewater / Q000737:chemistry*; D014920:Wine / Q000032:analysis*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D001713:Biphenyl Compounds; D005978:Glutathione; D017665:Hydroxyl Radical; D008315:Malondialdehyde; D010626:Phenylethyl Alcohol; D010851:Picrates; D013482:Superoxide Dismutase; D062065:Wastewater; C005975:3,4-dihydroxyphenylethanol; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Ferric reducing antioxidant power; hydroxyl radicals; malonaldehyde; protein carbonyl; superoxide dismutase