title: Bio-guided identification of proteins for the diagnosis of cysticercosis in swine.
abstract: Neurocysticercosis (NCC) is one of the most prevalent parasitic infection of the brain and the most common cause of seizures in adults in tropical countries. Cysticercosis is caused by larvae of Taenia solium, a human tapeworm. Pig or humans are infected by ingestion of eggs in food contaminated by human feces. Diagnosis and treatment of pigs is a pillar of the control of the disease in a country. However current diagnostic tests are based on ELISA and/or Western blot using native antigens needing laboratory facilities not available in rural areas. Development of a pen side diagnostic test for swines, makes sense. Immunochromatographic test should be adapted for this purpose. To design it we started a bio-guided identification of new proteins in cysticercus fluid. Proteins were analyzed using ion exchange chromatography and 2D separation and were selected by Western blot analysis using sera from infected/non infected pigs. Spots from the Coomassie-stained gel corresponding to these proteins were then analyzed by mass spectroscopy and proteins were identified using a bank of Expressed Sequence Tags (EST) of T. solium. Eighteen new proteins of interest were identified and nine were selected for further development.
authors.affiliation: Unité d'Immunologie, Institut Pasteur de Madagascar, Antananarivo, Madagascar, Madagascar. Electronic address: priscilla.nativel@hotmail.fr.
authors.forename: Priscilla
authors.initials: P
authors.lastname: Nativel
authors.affiliation: Unité d'Immunologie, Institut Pasteur de Madagascar, Antananarivo, Madagascar, Madagascar. Electronic address: anjanirina@pasteur.mg.
authors.forename: Anjanirina
authors.initials: A
authors.lastname: Rahantamalala
authors.affiliation: Unité d'Immunologie, Institut Pasteur de Madagascar, Antananarivo, Madagascar, Madagascar. Electronic address: sixcool@hotmail.com.
authors.forename: Sitraka
authors.initials: S
authors.lastname: Ramiandrisoa
authors.affiliation: Unité d'Immunologie, Institut Pasteur de Madagascar, Antananarivo, Madagascar, Madagascar. Electronic address: virginie.rasoamampianina@hotmail.com.
authors.forename: Virginie
authors.initials: V
authors.lastname: Rasoamampianinaa
authors.affiliation: Unité de Spectrométrie de Masse Structurale et Protéomique, Institut Pasteur, Paris, France. Electronic address: magalie.duchateau@pasteur.fr.
authors.forename: Magalie
authors.initials: M
authors.lastname: Duchateau
authors.affiliation: Unité de Spectrométrie de Masse Structurale et Protéomique, Institut Pasteur, Paris, France. Electronic address: julia.chamot-rooke@pasteur.fr.
authors.forename: Julia
authors.initials: J
authors.lastname: Chamot-Rooke
authors.affiliation: Unité d'Immunologie, Institut Pasteur de Madagascar, Antananarivo, Madagascar, Madagascar. Electronic address: rguebey@yahoo.fr.
authors.forename: Remy
authors.initials: R
authors.lastname: Guebey
authors.affiliation: FOFIFA-Département de Recherches Zootechniques et Vétérinaires/Faculté de Médecine- Département Vétérinaire, Antananarivo, Madagascar. Electronic address: harena23@yahoo.fr.
authors.forename: Harentsoaniaina
authors.initials: H
authors.lastname: Rasamoelina-Andriamanivo
authors.affiliation: Unité d'Immunologie, Institut Pasteur de Madagascar, Antananarivo, Madagascar, Madagascar. Electronic address: ronan.jambou@pasteur.fr.
authors.forename: Ronan
authors.initials: R
authors.lastname: Jambou
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000097:blood* / Q000235:genetics* / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000592:standards / Q000662:veterinary*; D003551:Cysticercosis / Q000097:blood / Q000175:diagnosis*; D003552:Cysticercus / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000175:diagnosis*
chemical_list: D000947:Antigens, Helminth
keywords: 2D electrophoresis; Cysticercosis; LC/MS-MS; Madagascar; Taenia solium