title: Protective effects of cyclosporine A and hypothermia on neuronal mitochondria in a rat asphyxial cardiac arrest model.
abstract: BACKGROUND
  Cyclosporine A (CsA) was neuroprotective in the settings of traumatic brain injury and stroke. We sought to investigate the protective effects of CsA and hypothermia on neuronal mitochondria after cardiac arrest.
  
  
  RESULTS
  Five groups were included: sham (S), normothermia (N), CsA (C), hypothermia (H), and CsA plus hypothermia (C+H). Cardiac arrest was induced by 10min of asphyxia. CsA (10mg/kg) was administered immediately after return of spontaneous circulation in the CsA groups. Temperature of the rats was maintained at 33±0.5°C after return of spontaneous circulation in the hypothermia groups. Hippocampal mitochondria were measured after 2h of resuscitation. Mitochondrial transmembrane potential was significantly higher in the C, the H, and the C+H groups than in the N group and was higher in the C+H group than in the C and the H groups. Cytosolic cytochrome c was significantly higher in the N group. Superoxide dismutase activity was significantly lower in the N group than in the other groups and was higher in the C and the C+H groups than in the H group. Malondialdehyde concentration was significantly higher in the N group.
  
  
  CONCLUSIONS
  CsA or hypothermia used immediately after resuscitation enhanced mitochondrial transmembrane potential, kept cytochrome c from releasing out of the mitochondria, increased superoxide dismutase activity, and decreased malondialdehyde concentration in hippocampus. Moreover, the protective effects of CsA were reinforced by hypothermia. One of the mechanisms that hypothermia protected neuronal mitochondria from damage was inhibiting the opening of mitochondrial permeability transition pore.
authors.affiliation: Department of Anesthesiology, the Third Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, China 150081.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology, the Third Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, China 150081.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, the Third Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, China 150081.
authors.forename: Qiwei
authors.initials: Q
authors.lastname: Zhuang
authors.affiliation: Department of Anesthesiology, the Third Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, China 150081.
authors.forename: Meng
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Anesthesiology, the Third Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, China 150081.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, the Third Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, China 150081.
authors.forename: Lina
authors.initials: L
authors.lastname: Hou
authors.affiliation: Department of Anesthesiology, the Third Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, China 150081. Electronic address: fh.feih@yahoo.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Han
mesh_terms: D000818:Animals; D001237:Asphyxia / Q000150:complications; D016572:Cyclosporine / Q000494:pharmacology*; D004195:Disease Models, Animal; D006323:Heart Arrest / Q000209:etiology / Q000473:pathology* / Q000628:therapy; D006624:Hippocampus / Q000187:drug effects* / Q000473:pathology; D007036:Hypothermia, Induced; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008315:Malondialdehyde / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects*; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D007166:Immunosuppressive Agents; D008315:Malondialdehyde; D016572:Cyclosporine; D013482:Superoxide Dismutase