title: Expression, localization and possible functions of aquaporins 3 and 8 in rat digestive system.
abstract: Although aquaporins (AQPs) play important roles in transcellular water movement, their precise quantification and localization remains controversial. We investigated expression levels and localizations of AQP3 and AQP8 and their possible functions in the rat digestive system using real-time polymerase chain reactions, western blot analysis and immunohistochemistry. We investigated the expression levels and localizations of AQP3 and AQP8 in esophagus, forestomach, glandular stomach, duodenum, jejunum, ileum, proximal and distal colon, and liver. AQP3 was expressed in the basolateral membranes of stratified epithelia (esophagus and forestomach) and simple columnar epithelia (glandular stomach, ileum, and proximal and distal colon). Expression was particularly abundant in the esophagus, and proximal and distal colon. AQP8 was found in the subapical compartment of columnar epithelial cells of the jejunum, ileum, proximal colon and liver; the most intense staining occurred in the jejunum. Our results suggest that AQP3 and AQP8 play significant roles in intestinal function and/or fluid homeostasis and may be an important subject for future investigation of disorders that involve disruption of intestinal fluid homeostasis, such as inflammatory bowel disease and irritable bowel syndrome.
authors.affiliation: a Department of Gastroenterology , Zhongshan Hospital of Fudan University , Xuhui District , Shanghai , China.
authors.forename: G X
authors.initials: GX
authors.lastname: Zhao
authors.affiliation: a Department of Gastroenterology , Zhongshan Hospital of Fudan University , Xuhui District , Shanghai , China.
authors.forename: P P
authors.initials: PP
authors.lastname: Dong
authors.affiliation: a Department of Gastroenterology , Zhongshan Hospital of Fudan University , Xuhui District , Shanghai , China.
authors.forename: R
authors.initials: R
authors.lastname: Peng
authors.affiliation: a Department of Gastroenterology , Zhongshan Hospital of Fudan University , Xuhui District , Shanghai , China.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.affiliation: a Department of Gastroenterology , Zhongshan Hospital of Fudan University , Xuhui District , Shanghai , China.
authors.forename: D Y
authors.initials: DY
authors.lastname: Zhang
authors.affiliation: a Department of Gastroenterology , Zhongshan Hospital of Fudan University , Xuhui District , Shanghai , China.
authors.forename: J Y
authors.initials: JY
authors.lastname: Wang
authors.affiliation: a Department of Gastroenterology , Zhongshan Hospital of Fudan University , Xuhui District , Shanghai , China.
authors.forename: X Z
authors.initials: XZ
authors.lastname: Shen
authors.affiliation: a Department of Gastroenterology , Zhongshan Hospital of Fudan University , Xuhui District , Shanghai , China.
authors.forename: L
authors.initials: L
authors.lastname: Dong
authors.affiliation: a Department of Gastroenterology , Zhongshan Hospital of Fudan University , Xuhui District , Shanghai , China.
authors.forename: J Y
authors.initials: JY
authors.lastname: Sun
mesh_terms: D000818:Animals; D051400:Aquaporin 3 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D020346:Aquaporins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D015153:Blotting, Western; D004064:Digestive System / Q000378:metabolism*; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D008297:Male; D021381:Protein Transport; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D020346:Aquaporins; C108330:aquaporin 8; D051400:Aquaporin 3
keywords: aquaporin 3; aquaporin 8; digestive system; expression; intestine; liver; localization; rat