title: Proinflammatory and Anti-Inflammatory Cytokines Mediated by NF-κB Factor as Prognostic Markers in Mammary Tumors.
abstract: Inflammation results in the production of cytokines, such as interleukin- (IL-) 4 and IL-10 with immunosuppressive properties or IL-6 and TNF-α with procarcinogenic activity. Furthermore, NF-κB is the major link between inflammation and tumorigenesis. This study verified the interaction between active inflammatory cytokines in the tumor microenvironment and serum of female dogs with mammary tumors and their correlation with the clinicopathological characteristics and overall survival. Measurement of gene expression was performed by qPCR and protein levels by ELISA/Luminex. High gene and protein expression levels of NF-κB, IL-6, and TNF-α were found in association with characteristics that reflect worse prognosis and a negative correlation between TNF-α protein expression and survival time was observed (p < 0.05). In contrast, high gene and protein expression levels of IL-4 and IL-10 were associated with characteristics of better prognosis and an increased level of IL-4 and a longer survival time of animals were obtained (p < 0.05). In addition, there was a positive correlation between TNF-α and IL-6 expression in association with NF-κB. The results show a significant correlation of these cytokines with tumor development, associated with NF-κB expression and cytokines promodulation, showing that these biological factors could be used as predictive and prognostic markers in breast cancer.
authors.affiliation: Laboratório de Investigação Molecular no Câncer (LIMC), Faculdade de Medicina de São José do Rio Preto (FAMERP), Department of Molecular Biology, Graduate Program in Health Science, Avenida Brigadeiro Faria Lima 5416, Vila São Pedro, 15090-000 São José do Rio Preto, SP, Brazil.
authors.forename: Gustavo Rodrigues
authors.initials: GR
authors.lastname: Martins
authors.affiliation: Laboratório de Investigação Molecular no Câncer (LIMC), Faculdade de Medicina de São José do Rio Preto (FAMERP), Department of Biology, Graduate Program in Genetics, Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP/IBILCE), Rua Cristóvão Colombo 2265, Jardim Nazareth, 15054-000 São José do Rio Preto, SP, Brazil.
authors.forename: Gabriela Bottaro
authors.initials: GB
authors.lastname: Gelaleti
authors.affiliation: Laboratório de Investigação Molecular no Câncer (LIMC), Faculdade de Medicina de São José do Rio Preto (FAMERP), Department of Molecular Biology, Graduate Program in Health Science, Avenida Brigadeiro Faria Lima 5416, Vila São Pedro, 15090-000 São José do Rio Preto, SP, Brazil.
authors.forename: Marina Gobbe
authors.initials: MG
authors.lastname: Moschetta
authors.affiliation: Laboratório de Investigação Molecular no Câncer (LIMC), Faculdade de Medicina de São José do Rio Preto (FAMERP), Department of Biology, Graduate Program in Genetics, Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP/IBILCE), Rua Cristóvão Colombo 2265, Jardim Nazareth, 15054-000 São José do Rio Preto, SP, Brazil.
authors.forename: Larissa Bazela
authors.initials: LB
authors.lastname: Maschio-Signorini
authors.affiliation: Laboratório de Investigação Molecular no Câncer (LIMC), Faculdade de Medicina de São José do Rio Preto (FAMERP), Avenida Brigadeiro Faria Lima 5416, Vila São Pedro, 15.090-000 São José do Rio Preto, SP, Brazil.
authors.forename: Debora Ap Pires de Campos
authors.initials: DA
authors.lastname: Zuccari
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism*; D004285:Dogs; D005260:Female; D016753:Interleukin-10 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; D015850:Interleukin-6; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D015847:Interleukin-4