title: A murine model of subglottic granulation.
abstract: OBJECTIVE
  This study aimed to develop a functional model of subglottic stenosis by inducing direct airway irritation in transplanted mouse laryngotracheal complexes.
  
  
  METHODS
  Laryngotracheal complexes from C57BL/6 mice were harvested and divided into three groups: uninjured, mechanically injured and chemically injured. Donor laryngotracheal complexes from each group were placed in dorsal subcutaneous pockets of recipient mice. Each week, the transplanted laryngotracheal complexes were harvested, and tissues were fixed, sectioned, and stained with haematoxylin and eosin. Representative slides were reviewed by a blinded pathologist, to determine the formation of granulation tissue, and graded as to the degree of granulation formation.
  
  
  RESULTS
  Direct airway irritation induced granulation tissue formation under the disrupted epithelium of airway mucosa; this was seen as early as two weeks after chemical injury.
  
  
  CONCLUSIONS
  Results indicate that granulation tissue formation in a murine model may be an efficient tool for investigating the development and treatment of subglottic stenosis.
authors.affiliation: Department of Otorhinolaryngology: Head and Neck Surgery,University of Pennsylvania,Philadelphia.
authors.forename: A
authors.initials: A
authors.lastname: Ghosh
authors.affiliation: Department of Otorhinolaryngology: Head and Neck Surgery,University of Pennsylvania,Philadelphia.
authors.forename: K P
authors.initials: KP
authors.lastname: Leahy
authors.affiliation: Department of Thoracic Surgery,University of Pennsylvania,Philadelphia.
authors.forename: S
authors.initials: S
authors.lastname: Singhal
authors.affiliation: Department of Pathology,Philadelphia Veterans' Affairs Medical Center,Pennsylvania,USA.
authors.forename: E
authors.initials: E
authors.lastname: Einhorn
authors.affiliation: Department of Pathology,Philadelphia Veterans' Affairs Medical Center,Pennsylvania,USA.
authors.forename: P
authors.initials: P
authors.lastname: Howlett
authors.affiliation: Department of Otorhinolaryngology: Head and Neck Surgery,University of Pennsylvania,Philadelphia.
authors.forename: N A
authors.initials: NA
authors.lastname: Cohen
authors.affiliation: Department of Otorhinolaryngology: Head and Neck Surgery,University of Pennsylvania,Philadelphia.
authors.forename: N
authors.initials: N
authors.lastname: Mirza
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006097:Granulation Tissue / Q000473:pathology*; D007829:Laryngostenosis / Q000139:chemically induced / Q000473:pathology*; D007830:Larynx / Q000473:pathology / Q000637:transplantation; D051379:Mice; D008810:Mice, Inbred C57BL; D014132:Trachea / Q000473:pathology / Q000637:transplantation
keywords: Acquired; Airway Obstruction; Animal Model; Granulation Tissue; Subglottic Stenosis