title: Blocking B7-1/CD28 Pathway Diminished Long-Range Brain Damage by Regulating the Immune and Inflammatory Responses in a Mouse Model of Intracerebral Hemorrhage.
abstract: Acute brain injuries can activate bidirectional crosstalk between the injured brain and the immune system. The immune system, particularly T lymphocytes and cytokines, has been implicated in the progression of brain injury after intracerebral hemorrhage (ICH). Co-stimulatory molecules B7-1 (CD80)/B7-2 (CD86) binding cognate receptor provides a secondary signaling to T cell activation. The aim of our study was to explore the effects of anti-B7-1 antibody on the development and prognosis of cerebral hemorrhage and to investigate the possible underlying mechanism. Mice were inner canthus veniplex administered with anti-B7-1 antibody at 10 min and 24 h after ICH and sacrificed on the third day after ICH. Immune function was assessed via splenocyte proliferation assay and organism index, respectively. IFN-γ and IL-4 were detected by enzyme-linked immuno sorbent assay. The cerebral edema was evaluated via brain water content. The levels of autophagy and apoptosis related proteins were measured by western blotting analysis. In addition, functional outcome was studied with pole-climbing test and morris water maze. The mice were weighed on 0, 1, 3, 14 and 21 days after ICH. The treatment with anti-B7-1 antibody significantly lowered immune function, and reduced the latency of water maze on 18 and 20 days, the ratio of IFN-γ/IL-4 as well as body weight on day 3 after cerebral hemorrhage. Our study suggests that in the cerebral hemorrhage mice brain anti-B7-1 antibody may reduce long-range brain damage by reversing immune imbalance.
authors.affiliation: Department of Forensic Medicine, Medical School of Soochow University, No. 178, Ganjiang East Road, Soochow, 215123, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Forensic Medicine, Medical School of Soochow University, No. 178, Ganjiang East Road, Soochow, 215123, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Shen
authors.affiliation: Department of Forensic Medicine, Medical School of Soochow University, No. 178, Ganjiang East Road, Soochow, 215123, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Forensic Medicine, Medical School of Soochow University, No. 178, Ganjiang East Road, Soochow, 215123, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Forensic Medicine, Medical School of Soochow University, No. 178, Ganjiang East Road, Soochow, 215123, China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Ji
authors.affiliation: Department of Forensic Medicine, Medical School of Soochow University, No. 178, Ganjiang East Road, Soochow, 215123, China.
authors.forename: Chengliang
authors.initials: C
authors.lastname: Luo
authors.affiliation: Department of Forensic Medicine, Medical School of Soochow University, No. 178, Ganjiang East Road, Soochow, 215123, China.
authors.forename: Mingyang
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Forensic Medicine, Medical School of Soochow University, No. 178, Ganjiang East Road, Soochow, 215123, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Forensic Medicine, Medical School of Soochow University, No. 178, Ganjiang East Road, Soochow, 215123, China.
authors.forename: Xiping
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Forensic Medicine, Medical School of Soochow University, No. 178, Ganjiang East Road, Soochow, 215123, China. luyang.tao@163.com.
authors.forename: Luyang
authors.initials: L
authors.lastname: Tao
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology / Q000627:therapeutic use; D018122:B7-1 Antigen / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000378:metabolism; D001930:Brain Injuries / Q000188:drug therapy / Q000276:immunology* / Q000378:metabolism; D018106:CD28 Antigens / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000378:metabolism; D002478:Cells, Cultured; D002543:Cerebral Hemorrhage / Q000188:drug therapy / Q000276:immunology* / Q000378:metabolism; D004195:Disease Models, Animal; D007111:Immunity, Cellular / Q000187:drug effects / Q000502:physiology*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000502:physiology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology
chemical_list: D000888:Antibodies, Anti-Idiotypic; D018122:B7-1 Antigen; D018106:CD28 Antigens
keywords: Anti-B7-1 antibody; Behavioristics; Cytokines; Immune; Intracerebral hemorrhage