title: A First Insight into the Genome of the Filter-Feeder Mussel Mytilus galloprovincialis.
abstract: Mussels belong to the phylum Mollusca, one of the largest and most diverse taxa in the animal kingdom. Despite their importance in aquaculture and in biology in general, genomic resources from mussels are still scarce. To broaden and increase the genomic knowledge in this family, we carried out a whole-genome sequencing study of the cosmopolitan Mediterranean mussel (Mytilus galloprovincialis). We sequenced its genome (32X depth of coverage) on the Illumina platform using three pair-end libraries with different insert sizes. The large number of contigs obtained pointed out a highly complex genome of 1.6 Gb where repeated elements seem to be widespread (~30% of the genome), a feature that is also shared with other marine molluscs. Notwithstanding the limitations of our genome sequencing, we were able to reconstruct two mitochondrial genomes and predict 10,891 putative genes. A comparative analysis with other molluscs revealed a gene enrichment of gene ontology categories related to multixenobiotic resistance, glutamate biosynthetic process, and the maintenance of ciliary structures.
authors.affiliation: Department of Biochemistry, Genetics and Immunology and Unidad Asociada CSIC, University of Vigo, Vigo, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Murgarella
authors.affiliation: Center for Computational Biology. McKusick-Nathans, Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.
authors.forename: Daniela
authors.initials: D
authors.lastname: Puiu
authors.affiliation: Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas, Vigo, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Novoa
authors.affiliation: Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas, Vigo, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Figueras
authors.affiliation: Department of Biochemistry, Genetics and Immunology and Unidad Asociada CSIC, University of Vigo, Vigo, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Posada
authors.affiliation: Department of Biochemistry, Genetics and Immunology and Unidad Asociada CSIC, University of Vigo, Vigo, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Canchaya
mesh_terms: D000220:Adaptation, Biological / Q000235:genetics; D000818:Animals; D003198:Computer Simulation; D020451:Contig Mapping; D004272:DNA, Mitochondrial / Q000235:genetics; D015723:Gene Library; D063990:Gene Ontology; D016678:Genome; D054629:Genome, Mitochondrial; D059014:High-Throughput Nucleotide Sequencing; D008957:Models, Genetic; D058977:Molecular Sequence Annotation; D008974:Mollusca / Q000235:genetics; D049878:Mytilus / Q000235:genetics* / Q000502:physiology; D012091:Repetitive Sequences, Nucleic Acid; D016415:Sequence Alignment; D013045:Species Specificity
chemical_list: D004272:DNA, Mitochondrial