title: Carbapenemase-producing Enterobacteriaceae: a 2-year surveillance in a hospital in Iaşi, Romania.
abstract: OBJECTIVE
  Limited information is currently available about the prevalence of carbapenemase-producing Enterobacteriaceae (CPE) in Romania.
  
  
  METHODS
  Routine tests of 1,993 clinical isolates at a hospital in Iaşi yielded 46 isolates that were resistant to carbapenems. All 46 isolates were phenotypically and genotypically analyzed using VITEK-2 and DNA microarray-based assays.
  
  
  RESULTS
  Isolates were assigned to Klebsiella pneumoniae and Enterobacter cloacae. For 39 isolates, carbapenem resistance was confirmed and 37 harbored at least one carbapenem resistance gene. Two isolates were probably resistant due to AmpC β-lactamases in combination with a porin loss. The overall concordance between detected phenotype and genotype was 95%.
  
  
  CONCLUSIONS
  Our data show that carbapenemase-producing isolates with different underlying resistance mechanisms are still rare in Iaşi, but the global rise of CPE warrants intensified surveillance.
authors.affiliation: Alere Technologies GmbH, Jena, Germany.|InfectoGnostics Research Campus, Jena, Germany.
authors.forename: Sascha D
authors.initials: SD
authors.lastname: Braun
authors.affiliation: University of Medicine & Pharmacy ''Grigore T Popa,'' Iaşi, Romania.
authors.forename: Olivia S
authors.initials: OS
authors.lastname: Dorneanu
authors.affiliation: University of Medicine & Pharmacy ''Grigore T Popa,'' Iaşi, Romania.
authors.forename: Teodora
authors.initials: T
authors.lastname: Vremeră
authors.affiliation: Alere Technologies GmbH, Jena, Germany.|InfectoGnostics Research Campus, Jena, Germany.
authors.forename: Annett
authors.initials: A
authors.lastname: Reißig
authors.affiliation: Alere Technologies GmbH, Jena, Germany.|InfectoGnostics Research Campus, Jena, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Monecke
authors.affiliation: Alere Technologies GmbH, Jena, Germany.|InfectoGnostics Research Campus, Jena, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Ehricht
mesh_terms: D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D016972:Enterobacter cloacae / Q000187:drug effects / Q000235:genetics; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000453:epidemiology* / Q000382:microbiology* / Q000401:mortality; D062665:Epidemiological Monitoring; D005260:Female; D005838:Genotype; D006761:Hospitals / Q000706:statistics & numerical data*; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D010641:Phenotype; D015995:Prevalence; D012383:Romania / Q000453:epidemiology; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase
keywords: CPE; DNA microarray; Romania; carbapenem resistance; carbapenemase-producing Enterobacteriaceae; clinical isolates; genotyping; phenotyping