title: Biosynthesis of Monascus pigments by resting cell submerged culture in nonionic surfactant micelle aqueous solution.
abstract: Growing cell submerged culture is usually applied for fermentative production of intracellular orange Monascus pigments, in which accumulation of Monascus pigments is at least partially associated to cell growth. In the present work, extractive fermentation in a nonionic surfactant micelle aqueous solution was utilized as a strategy for releasing of intracellular Monascus pigments. Those mycelia with low content of intracellular Monascus pigments were utilized as biocatalyst in resting cell submerged culture. By this means, resting cell submerged culture for production of orange Monascus pigments was carried out successfully in the nonionic surfactant micelle aqueous solution, which exhibited some advantages comparing with the corresponding conventional growing cell submerged culture, such as non-sterilization operation, high cell density (24 g/l DCW) leading to high productivity (14 AU of orange Monascus pigments at 470 nm per day), and recycling of cells as biocatalyst leading to high product yield (approximately 1 AU of orange Monascus pigments at 470 nm per gram of glucose) based on energy metabolism.
authors.affiliation: School of Pharmacy, State Key Laboratory of Microbial Metabolism, and Engineering Research Center of Cell & Therapeutic Antibody, Ministry of Education, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China.|School of Biological Science and Engineering, South China University of Technology, Guangzhou, 510006, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: School of Life Science and Biotechnology, and State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China.
authors.forename: Xuehong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Biological Science and Engineering, South China University of Technology, Guangzhou, 510006, People's Republic of China.
authors.forename: Zhenqiang
authors.initials: Z
authors.lastname: Wu
authors.affiliation: School of Pharmacy, State Key Laboratory of Microbial Metabolism, and Engineering Research Center of Cell & Therapeutic Antibody, Ministry of Education, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China. zlwang@sjtu.edu.cn.
authors.forename: Zhilong
authors.initials: Z
authors.lastname: Wang
mesh_terms: D001688:Biological Products / Q000378:metabolism*; D018929:Cell Culture Techniques; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D008823:Micelles; D032741:Monascus / Q000378:metabolism*; D025282:Mycelium / Q000378:metabolism; D017830:Octoxynol / Q000494:pharmacology; D010860:Pigments, Biological / Q000096:biosynthesis*; D013501:Surface-Active Agents / Q000378:metabolism*
chemical_list: D001688:Biological Products; D008823:Micelles; D010860:Pigments, Biological; D013501:Surface-Active Agents; D017830:Octoxynol; D005947:Glucose
keywords: Extractive fermentation; Intracellular product; Monascus pigments; Nonionic surfactant; Resting cells