title: A green analytical method for rapid determination of pectin degree of esterification using micro sequential injection lab-on-valve system.
abstract: A novel method for automated determination pectin degree of esterification (DE) using micro sequential injection lab-on-valve (μSI-LOV) system is developed. A face-centered central composite response surface methodology (RSM) was used to optimise system parameters. A calibration graph for determination of non-esterified galacturonic acid (GalA) content in pectin solutions with linear range of 0.08-0.34% (w/v) and the limit of detection (LOD) of 0.057% (w/v) under optimal condition was achieved. The difference between concentrations (w/v, %) of total GalA and non-esterified GalA was applied to estimate DE (%) of pectin samples. Results indicated a good agreement (tstat<tcrit) and correlation (R(2)=0.998) between proposed automated method and the manual reference method. The current method provided precision of less than 6% RSD, (n=10) with sample throughput of 15 samples h(-1). The presented method requires remarkably low consumption of sample and reagents and is thereby environmental friendly.
authors.affiliation: Department of Food Science, Faculty of Science, University of Copenhagen, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark; Chemistry Laboratory, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece.
authors.forename: Mahsa
authors.initials: M
authors.lastname: Naghshineh
authors.affiliation: CP Kelco, Ved Banen 16, 4623 Lille Skensved, Denmark.
authors.forename: Jan
authors.initials: J
authors.lastname: Larsen
authors.affiliation: Chemistry Laboratory, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece.
authors.forename: Constantinos
authors.initials: C
authors.lastname: Georgiou
authors.affiliation: Department of Food Science, Faculty of Science, University of Copenhagen, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark. Electronic address: ko@food.ku.dk.
authors.forename: Karsten
authors.initials: K
authors.lastname: Olsen
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000295:instrumentation / Q000379:methods*; D004951:Esterification; D055772:Green Chemistry Technology / Q000295:instrumentation; D057230:Limit of Detection; D010368:Pectins / Q000737:chemistry*
chemical_list: D010368:Pectins
keywords: Degree of esterification; Green analytical method; Micro sequential injection analysis; Non-esterified galacturonic acid; Pectin; Response surface methodology