title: Liver Transcriptome Analysis of the Large Yellow Croaker (Larimichthys crocea) during Fasting by Using RNA-Seq.
abstract: The large yellow croaker (Larimichthys crocea) is an economically important fish species in Chinese mariculture industry. To understand the molecular basis underlying the response to fasting, Illumina HiSeqTM 2000 was used to analyze the liver transcriptome of fasting large yellow croakers. A total of 54,933,550 clean reads were obtained and assembled into 110,364 contigs. Annotation to the NCBI database identified a total of 38,728 unigenes, of which 19,654 were classified into Gene Ontology and 22,683 were found in Kyoto Encyclopedia of Genes and Genomes (KEGG). Comparative analysis of the expression profiles between fasting fish and normal-feeding fish identified a total of 7,623 differentially expressed genes (P < 0.05), including 2,500 upregulated genes and 5,123 downregulated genes. Dramatic differences were observed in the genes involved in metabolic pathways such as fat digestion and absorption, citrate cycle, and glycolysis/gluconeogenesis, and the similar results were also found in the transcriptome of skeletal muscle. Further qPCR analysis confirmed that the genes encoding the factors involved in those pathways significantly changed in terms of expression levels. The results of the present study provide insights into the molecular mechanisms underlying the metabolic response of the large yellow croaker to fasting as well as identified areas that require further investigation.
authors.affiliation: College of Marine Sciences, Ningbo University, Ningbo, Zhejiang, China.|School of life science, Taizhou University, Taizhou, Zhejiang, China.
authors.forename: Baoying
authors.initials: B
authors.lastname: Qian
authors.affiliation: College of Marine Sciences, Ningbo University, Ningbo, Zhejiang, China.|Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo University, Ningbo, Zhejiang, China.
authors.forename: Liangyi
authors.initials: L
authors.lastname: Xue
authors.affiliation: College of Marine Sciences, Ningbo University, Ningbo, Zhejiang, China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Huang
mesh_terms: D000818:Animals; D005215:Fasting; D029941:Fish Proteins / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D063990:Gene Ontology; D008099:Liver / Q000473:pathology; D018482:Muscle, Skeletal / Q000378:metabolism; D010473:Perciformes / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods*
chemical_list: D029941:Fish Proteins