title: Hydroxyl-modified magnetite nanoparticles as novel carrier for delivery of methotrexate.
abstract: In this work, novel hydroxyl-modified magnetite nanocarriers are introduced as efficient host for methotrexate conjugation. The modification was based on the Micheal type addition reaction between tris(hydroxymethyl) aminomethane and acrylamidopropyl functionalized, silica-coated magnetite nanoparticle. The chemical structure characterization was carried out by FT-IR and the organic content was determined by CHN analysis. The topography was studied by SEM, TEM, AFM. DLS was performed to show particles' mean diameter. Furthermore, the magnetite properties of modified particles were evaluated by VSM and the crystallinity was proved by XRD. To illustrate the efficiency of the modified particles, the anti-cancer drug methotrexate was conjugated to hydroxyl groups through estric bond formation. The controlled release activity of established nanoparticles was evaluated in simulated cellular fluid. Later, the anti-cancer behavior of drug conjugated nanoparticles was evaluated in vitro in MCF-7 cell line which showed enhanced toxicity after 48 h. Conclusively, the modified nanoparticles have remarked as powerful carrier to be applied as an anti-cancer agent.
authors.affiliation: Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Shiraz University of Medical Sciences, P.O. Box 71345-1583, Shiraz, Iran. Electronic address: farjadian_f@sums.ac.ir.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Farjadian
authors.affiliation: Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Shiraz University of Medical Sciences, P.O. Box 71345-1583, Shiraz, Iran.
authors.forename: Sahar
authors.initials: S
authors.lastname: Ghasemi
authors.affiliation: Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Shiraz University of Medical Sciences, P.O. Box 71345-1583, Shiraz, Iran; Department of Pharmaceutics, Faculty of Pharmacy, Shiraz University of Medical Sciences, P.O. Box 71345-1583, Shiraz, Iran. Electronic address: smsamani@sums.ac.ir.
authors.forename: Soliman
authors.initials: S
authors.lastname: Mohammadi-Samani
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000737:chemistry; D004337:Drug Carriers / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D006878:Hydroxides / Q000008:administration & dosage* / Q000737:chemistry; D061986:MCF-7 Cells; D058185:Magnetite Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D008727:Methotrexate / Q000008:administration & dosage* / Q000737:chemistry; D012822:Silicon Dioxide / Q000008:administration & dosage / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D006878:Hydroxides; D058185:Magnetite Nanoparticles; D012822:Silicon Dioxide; C031356:hydroxide ion; D008727:Methotrexate
keywords: Anti-cancer; Drug delivery; MCF-7; Magnetic nanoparticle (MNP); Magnetite nanoparticles; Methotrexate (MTX)