title: Identification of Mycobacterial Antigens in Human Urine by Use of Immunoglobulin G Isolated from Sera of Patients with Active Pulmonary Tuberculosis.
abstract: Point-of-care (POC) diagnostic testing of tuberculosis (TB) is a tremendous unmet need. In this study, four urinary mycobacterial antigens were identified through two independent approaches using IgG capture and immunodepletion methods. Among these, ModC was validated by a multiple reaction monitoring (MRM) method. As expected, the biomarkers elevated the clinical validity of TB diagnosis when combined with preexisting markers.
authors.affiliation: Genome Editing Research Center, KRIBB, Deajeon, South Korea.
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Genome Editing Research Center, KRIBB, Deajeon, South Korea.
authors.forename: Nan-Ee
authors.initials: NE
authors.lastname: Lee
authors.affiliation: International Tuberculosis Research Center, Changwon-si, South Korea.
authors.forename: Jong Seok
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of Laboratory Medicine and Paik Institute for Clinical Research, Inje University College of Medicine, Busan, South Korea.
authors.forename: Jeong Hwan
authors.initials: JH
authors.lastname: Shin
authors.affiliation: Division of Mass Spectrometry, Korea Basic Science Institute, Cheongwon-Gun, South Korea.
authors.forename: Ju Yeon
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Genome Editing Research Center, KRIBB, Deajeon, South Korea.|Korea University of Science and Technology, Daejeon, South Korea.
authors.forename: Jeong-Heon
authors.initials: JH
authors.lastname: Ko
authors.affiliation: Department of Laboratory Medicine, School of Medicine, Pusan National University, Busan, South Korea omsys1@kribb.re.kr cchl@pusan.ac.kr.
authors.forename: Chulhun Ludgerus
authors.initials: CL
authors.lastname: Chang
authors.affiliation: Genome Editing Research Center, KRIBB, Deajeon, South Korea omsys1@kribb.re.kr cchl@pusan.ac.kr.|Korea University of Science and Technology, Daejeon, South Korea.
authors.forename: Yong-Sam
authors.initials: YS
authors.lastname: Kim
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology*; D000942:Antigens, Bacterial / Q000652:urine*; D015415:Biomarkers / Q000652:urine*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000276:immunology*; D009161:Mycobacterium / Q000737:chemistry*; D019095:Point-of-Care Systems; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D015415:Biomarkers; D007074:Immunoglobulin G