title: Sequential recycling of enzymatic lipid-extracted hydrolysate in fermentations with a thraustochytrid.
abstract: This study extends the findings of prior studies proposing and validating nutrient recycling for the heterotrophic microalgae, Thraustochytrium sp. (T18), grown in optimized fed-batch conditions. Sequential nutrient recycling of enzymatically-derived hydrolysate in fermentors succeeded at growing the tested thraustochytrid strain, with little evidence of inhibition or detrimental effects upon culture health. The average maximum biomass obtained in the recycled hydrolysate was 63.68±1.46gL(-1) in 90h the first recycle followed by 65.27±1.15gL(-1) in 90h in the subsequent recycle of the same material. These compared to 58.59gL(-1) and 64.92gL(-1) observed in fresh media in the same time. Lipid production was slightly impaired, however, with a maximum total fatty acid content of 62.2±0.30% in the recycled hydrolysate compared to 69.4% in fresh control media.
authors.affiliation: Department of Process Engineering and Applied Science, Faculty of Engineering, Dalhousie University, Halifax, Nova Scotia B3H 4R2, Canada. Electronic address: Joshua.Lowrey@Dal.ca.
authors.forename: Joshua
authors.initials: J
authors.lastname: Lowrey
authors.affiliation: Department of Process Engineering and Applied Science, Faculty of Engineering, Dalhousie University, Halifax, Nova Scotia B3H 4R2, Canada; Mara Renewables Corporation, 101 Research Drive, Dartmouth, Nova Scotia B2Y 4T6, Canada.
authors.forename: Roberto E
authors.initials: RE
authors.lastname: Armenta
authors.affiliation: Department of Process Engineering and Applied Science, Faculty of Engineering, Dalhousie University, Halifax, Nova Scotia B3H 4R2, Canada.
authors.forename: Marianne S
authors.initials: MS
authors.lastname: Brooks
mesh_terms: D018533:Biomass; D019149:Bioreactors; D005227:Fatty Acids; D005285:Fermentation; D052836:Heterotrophic Processes; D008055:Lipids; D058086:Microalgae / Q000378:metabolism*; D011492:Protein Hydrolysates / Q000737:chemistry*; D059027:Recycling / Q000379:methods; D058009:Stramenopiles / Q000737:chemistry*
chemical_list: D005227:Fatty Acids; D008055:Lipids; D011492:Protein Hydrolysates
keywords: Amino acids; Enzyme hydrolysate; Heterotrophic microalgae; Nitrogen recycling; Sequential nutrient recycling