title: Quantification of leucocytes, T-lymphocytes and macrophages in autoptical endomyocardial tissue from 56 normal human hearts during the first year of life.
abstract: This study evaluated the normal number of inflammatory cells in the heart in the first year of life using two methods to compare their ability to quantitate physiological myocardial infiltration. Eight endomyocardial samples from both ventricles were obtained at autopsy from 56 structurally normal hearts during the first year of life. In each sample the numbers of leucocytes, T-lymphocytes and macrophages were counted once in 20 randomly chosen high-power fields (400×) as well as in a 10mm(2) area of randomly chosen myocardial tissue (100×) by two independent investigators. Compared to the literature a greater representative proportion of myocardial tissue was analyzed. The results of the enumeration in mm(2) were converted into high-power-fields to compare both methods. The mean numbers and standard deviations for leucocytes, T-lymphocytes and macrophages were calculated. Both counting methods showed similar results with low inflammatory cell counts per single heart and staining. A greater understanding of the physiological myocardial infiltration by leucocytes, T-lymphocytes and macrophages is important for postmortem forensic cases, and for the interpretation of endomyocardial biopsies in infants.
authors.affiliation: Institute of Forensic Medicine, University of Bonn, Stiftsplatz 12, D-53111 Bonn, Germany.
authors.forename: Sarah
authors.initials: S
authors.lastname: Grasmeyer
authors.affiliation: Institute of Forensic Medicine, University of Bonn, Stiftsplatz 12, D-53111 Bonn, Germany.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Oswald
authors.affiliation: Institute of Forensic Medicine, University of Bonn, Stiftsplatz 12, D-53111 Bonn, Germany. Electronic address: b.madea@uni-bonn.de.
authors.forename: Burkhard
authors.initials: B
authors.lastname: Madea
mesh_terms: D002452:Cell Count; D049429:Forensic Pathology; D006801:Humans; D007223:Infant; D007962:Leukocytes; D008264:Macrophages; D009206:Myocardium / Q000473:pathology*; D013601:T-Lymphocytes / Q000378:metabolism*
keywords: Endomyocardial autoptical samples; First year of life; Infants; Myocarditis; Normal hearts