title: The complete mitochondrial genome of Gasterophilus intestinalis, the first representative of the family Gasterophilidae.
abstract: Gasterophilus spp. (Diptera: Gasterophilidae) has a worldwide distribution; however, no complete mitochondrial (mt) genome data is available for Diptera which has greatly impeded population genetics, phylogenetics, and systematics studies in Gasterophilidae. Mt genome is known to provide genetic markers for investigations in these areas, but complete mt genomic datasets have been lacking for many Gasterophilidae species. Herein, we present the complete mt genome of the third-stage larvae (L3) of Gasterophilus intestinalis from the stomach wall of naturally infected horses in Heilongjiang province (HLJ) and Xinjiang Uygur Autonomous Region (XJ), China. The complete mt genome of G. intestinalis was 15,687 bp (HLJ) and 15,660 bp (XJ) in length and consists of 37 genes, including 13 genes for proteins, 22 genes for tRNA, and 2 genes for rRNA. The gene arrangement is the same as those of Oestroidae species. Phylogenetic analyses using concatenated amino acid sequences of 12 protein-coding genes by Bayesian inference (BI) and maximum likelihood (ML), suggested that the families Gasterophilidae and Oestroidae were more closely related than to Tachinidae. The mt genome of G. intestinalis represents the first mt genome of any member of the family Gasterophilidae. These data provide novel mtDNA markers for studying the molecular epidemiology and population genetics of the G. intestinalis and its congeners.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, 730046, People's Republic of China.|College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province, 163319, People's Republic of China.
authors.forename: De-Zhen
authors.initials: DZ
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, 730046, People's Republic of China. liuguohua5202008@163.com.
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, 730046, People's Republic of China.
authors.forename: Hui-Qun
authors.initials: HQ
authors.lastname: Song
authors.affiliation: Veterinary Research Institute, Xinjiang Academy of Animal Sciences, Urumqi, Xinjiang Uyghur Autonomous Region, 830000, People's Republic of China.
authors.forename: Guang-Lei
authors.initials: GL
authors.lastname: Wang
authors.affiliation: College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province, 163319, People's Republic of China.
authors.forename: Chun-Ren
authors.initials: CR
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, 730046, People's Republic of China. xingquanzhu1@hotmail.com.|College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province, 163319, People's Republic of China. xingquanzhu1@hotmail.com.|Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University College of Veterinary Medicine, Yangzhou, Jiangsu Province, 225009, People's Republic of China. xingquanzhu1@hotmail.com.
authors.forename: Xing-Quan
authors.initials: XQ
authors.lastname: Zhu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002681:China; D004272:DNA, Mitochondrial / Q000235:genetics*; D004175:Diptera / Q000145:classification* / Q000235:genetics*; D023061:Gene Order; D005819:Genetic Markers; D054629:Genome, Mitochondrial / Q000235:genetics*; D006736:Horses / Q000469:parasitology*; D007814:Larva / Q000235:genetics; D010271:Parasites / Q000145:classification / Q000235:genetics / Q000254:growth & development; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D013270:Stomach / Q000469:parasitology* / Q000473:pathology
chemical_list: D004272:DNA, Mitochondrial; D005819:Genetic Markers; D012335:RNA, Ribosomal; D012343:RNA, Transfer
keywords: Gasterophilus; Intestinalis; Mitochondrial DNA; Mitochondrial genome; Phylogenetic analyses