title: Isolation, identification, and crude oil degradation characteristics of a high-temperature, hydrocarbon-degrading strain.
abstract: In this work, a hydrocarbon-degrading bacterium Y-1 isolated from petroleum contaminated soil in the Dagang Oilfield was investigated for its potential effect in biodegradation of crude oil. According to the analysis of 16S rRNA sequences, strain Y-1 was identified as Bacillus licheniformis. The growth parameters such as pH, temperature, and salinity were optimised and 60.2% degradation of crude oil removal was observed in 5days. The strain Y-1 showed strong tolerance to high salinity, alkalinity, and temperature. Emplastic produced by strain Y-1 at high temperatures could be applied as biosurfactant. Gas chromatography analysis demonstrated that the strain Y-1 efficiently degraded different alkanes from crude oil, and the emplastic produced by strain Y-1 promoted the degradation rates of long-chain alkanes when the temperature increased to 55°C. Therefore, strain Y-1 would play an important role in the area of crude oil contaminant bioremediation even in some extreme conditions.
authors.affiliation: Tianjin Biomass Solid Waste Reclamation Technology and Engineering Center, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, PR China.
authors.forename: Boqun
authors.initials: B
authors.lastname: Liu
authors.affiliation: Tianjin Biomass Solid Waste Reclamation Technology and Engineering Center, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, PR China. Electronic address: jumeit@nankai.edu.cn.
authors.forename: Meiting
authors.initials: M
authors.lastname: Ju
authors.affiliation: Tianjin Biomass Solid Waste Reclamation Technology and Engineering Center, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, PR China.
authors.forename: Jinpeng
authors.initials: J
authors.lastname: Liu
authors.affiliation: Tianjin Biomass Solid Waste Reclamation Technology and Engineering Center, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, PR China; Department of Agricultural and Biological Engineering, Pennsylvania State University, University Park, PA 16801, United States.
authors.forename: Wentao
authors.initials: W
authors.lastname: Wu
authors.affiliation: MOE Key Laboratory of Pollution Processes and Environmental Criteria, Nankai University, No 94 Weijin Road, PR China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Li
mesh_terms: D000473:Alkanes / Q000032:analysis* / Q000378:metabolism; D000069976:Bacillus licheniformis / Q000302:isolation & purification* / Q000378:metabolism; D001673:Biodegradation, Environmental; D008962:Models, Theoretical; D061329:Oil and Gas Fields; D010578:Petroleum / Q000032:analysis* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013696:Temperature; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000378:metabolism
chemical_list: D000473:Alkanes; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical
keywords: Bacillus licheniformis; Biodegradation; Biosurfactant; Crude oil; Emplastic; High temperature