title: Low Temperature Treatment Affects Concentration and Distribution of Chrysanthemum Stunt Viroid in Argyranthemum.
abstract: Chrysanthemum stunt viroid (CSVd) can infect Argyranthemum and cause serious economic loss. Low temperature treatment combined with meristem culture has been applied to eradicate viroids from their hosts, but without success in eliminating CSVd from diseased Argyranthemum. The objectives of this work were to investigate (1) the effect of low temperature treatment combined with meristem culture on elimination of CSVd, (2) the effect of low temperature treatment on CSVd distribution pattern in shoot apical meristem (SAM), and (3) CSVd distribution in flowers and stems of two infected Argyranthemum cultivars. After treatment with low temperature combined with meristem tip culture, two CSVd-free plants were found in 'Border Dark Red', but none in 'Yellow Empire'. With the help of in situ hybridization, we found that CSVd distribution patterns in the SAM showed no changes in diseased 'Yellow Empire' following 5°C treatment, compared with non-treated plants. However, the CSVd-free area in SAM was enlarged in diseased 'Border Dark Red' following prolonged 5°C treatment. Localization of CSVd in the flowers and stems of infected 'Border Dark Red' and 'Yellow Empire' indicated that seeds could not transmit CSVd in these two cultivars, and CSVd existed in phloem. Results obtained in the study contributed to better understanding of the distribution of CSVd in systemically infected plants and the combination of low temperature treatment and meristem tip culture for production of viroid-free plants.
authors.affiliation: The Plant Health and Biotechnology Division, Norwegian Institute of Bioeconomy ResearchÅs, Norway; State Key Laboratory of Crop Stress Biology for Arid Areas, Key Laboratory of Genetic Improvement of Horticultural Crops of Northwest China, Department of Plant Sciences, College of Horticulture, Northwest A&F UniversityYangling, China.
authors.forename: Zhibo
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Plant Sciences, Norwegian University of Life Science Ås, Norway.
authors.forename: YeonKyeong
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Department of Plant Sciences, Norwegian University of Life Science Ås, Norway.
authors.forename: Astrid
authors.initials: A
authors.lastname: Sivertsen
authors.affiliation: Department of Plant Sciences, Norwegian University of Life Science Ås, Norway.
authors.forename: Gry
authors.initials: G
authors.lastname: Skjeseth
authors.affiliation: The Plant Health and Biotechnology Division, Norwegian Institute of Bioeconomy Research Ås, Norway.
authors.forename: Sissel
authors.initials: S
authors.lastname: Haugslien
authors.affiliation: The Plant Health and Biotechnology Division, Norwegian Institute of Bioeconomy Research Ås, Norway.
authors.forename: Jihong Liu
authors.initials: JL
authors.lastname: Clarke
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, Key Laboratory of Genetic Improvement of Horticultural Crops of Northwest China, Department of Plant Sciences, College of Horticulture, Northwest A&F University Yangling, China.
authors.forename: Qiao-Chun
authors.initials: QC
authors.lastname: Wang
authors.affiliation: The Plant Health and Biotechnology Division, Norwegian Institute of Bioeconomy Research Ås, Norway.
authors.forename: Dag-Ragnar
authors.initials: DR
authors.lastname: Blystad
keywords: CSVd; in situ hybridization; meristem culture; shoot apical meristem; viroid localization