title: Evaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cells.
abstract: This in vitro study was conducted to evaluate lymphocyte blastogenic and cytokine production by bovine peripheral blood mononuclear cells (PBMCs) stimulated with phytohemagglutinin (PHA), pokeweed mitogen (PWM) and concanavalin A (Con A) mitogens, by using tetrazolium salt and ELISA tests, respectively. The results presented that Interleukin-2 (IL-2), IL-4, IL-5, IL-10, IL-17 and IFN-γ production in response to PWM mitogens was the highest and Con A the lowest amount and the median values of three mitogens were in the following order: PWM > PHA > Con A > cell control. In the case of IL-6, the production of this cytokine was the same amount for PWM and Con A and a lower amount for PHA stimulation. The results of this study not only showed a normal range for the production of these cytokines from PBMCs that were affected by mitogens, but it demonstrated that the bovine immune system at 2.5 to 3 months was post-natally matured enough to mount an effective immune response to mitogens as well as specific antigens.
authors.affiliation: Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;
authors.forename: Reza
authors.initials: R
authors.lastname: Norian
authors.affiliation: Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;
authors.forename: Nowruz
authors.initials: N
authors.lastname: Delirezh
authors.affiliation: Department of Immunology, Qazvin University of Medical Sciences, Qazvin, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Azadmehr
keywords: Bovine; Concanavalin A; Cytokine; Phytohemagglutinin; Pokeweed mitogen