title: Brief Communication: Discordant ability of the triglyceride to apolipoprotein B ratio to predict triglyceride-rich lipoprotein particle size in normal-weight and obese men.
abstract: The atherogenicity of triglyceride-rich lipoproteins (TRLs) is dependent of their particle size as it determines their metabolic fate. Since TRL possess a single apolipoprotein B (Apo B) molecule per particle, the triglyceride (TG)/Apo B ratio has been used as a convenient method to estimate TRL size. The aim of this study was to validate this approach by correlating the serum TG/Apo B ratio, and the TRL particle size measured by dynamic light scattering (DLS). Twenty-four male volunteers (12 normal-weight and 12 obese individuals) received a high-fat meal. Preprandial (0 h) and postprandial (2 and 4 h) serum samples were collected after meal ingestion, and TRLs were isolated. Serum TG and Apo B levels were quantified, and the TG/Apo B ratio was plotted against TRL particle size measured by DLS for correlation. A strong association between TRL particle size and serum TG/Apo B ratio for normal-weight subjects (P ≤ 0.001) was observed but not for obese subjects (P = 0.6116). TG/Apo B ratio correlates with particle size in healthy normal-weight males but not in obese individuals. Whether this ratio is useful to estimate TRL size in females and in other dyslipidemic patients should be subject of future investigations.
authors.affiliation: Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (IG-CSIC), Campus Universidad Pablo de Olavide, Ctra. Utrera, km1. E-41013 Sevilla, Spain.
authors.forename: Miryam
authors.initials: M
authors.lastname: Amigo-Benavent
authors.affiliation: Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (IG-CSIC), Campus Universidad Pablo de Olavide, Ctra. Utrera, km1. E-41013 Sevilla, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Sinausia
authors.affiliation: HHUU Virgen del Rocío. Av. Manuel Siurot s/n. E-41013. Seville, Spain.
authors.forename: Emilio
authors.initials: E
authors.lastname: Montero
authors.affiliation: Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (IG-CSIC), Campus Universidad Pablo de Olavide, Ctra. Utrera, km1. E-41013 Sevilla, Spain perona@ig.csic.es.
authors.forename: Javier S
authors.initials: JS
authors.lastname: Perona
mesh_terms: D000328:Adult; D001055:Apolipoproteins B / Q000097:blood*; D015992:Body Mass Index; D016022:Case-Control Studies; D004041:Dietary Fats / Q000494:pharmacology; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008297:Male; D009765:Obesity / Q000097:blood* / Q000378:metabolism; D010316:Particle Size; D014280:Triglycerides / Q000097:blood*
chemical_list: D001055:Apolipoproteins B; D004041:Dietary Fats; D008074:Lipoproteins; D014280:Triglycerides
keywords: Apolipoprotein B; particle size; ratio; triglyceride; triglyceride-rich lipoprotein