title: Ginger hexane extract suppresses RANKL-induced osteoclast differentiation.
abstract: Osteoporosis is a debilitating disease caused by decreased bone density. Compounds with anti-osteoclastic activity, such as bisphosphonates, may help in the prevention and treatment of osteoporosis. Herein, we determined the inhibitory effects of ginger hexane extract (GHE) on receptor activator of nuclear factor kappa-B ligand (RANKL)-induced osteoclastogenesis in RAW264.7 cells. The results showed that GHE (1) suppressed osteoclast differentiation and the formation of actin rings; (2) inhibited the expression of Nfatc1, a master transcriptional factor for osteoclast differentiation, in a dose-dependent manner (10-20 μg/mL); and (3) inhibited other osteoclastogenesis-related genes, such as Oscar, Dc-stamp, Trap, and Mmp9. These findings suggest that GHE may be used to prevent and treat osteoporosis by inhibiting osteoclast differentiation.
authors.affiliation: a Tsuji Health & Beauty Science Laboratory , Mie University , Tsu, Japan.|b Tsuji Oil Mills Co., Ltd. , Matsusaka , Japan.|c Graduate School of Regional Innovation Studies , Mie University , Tsu, Japan.
authors.forename: Suguru
authors.initials: S
authors.lastname: Ito
authors.affiliation: b Tsuji Oil Mills Co., Ltd. , Matsusaka , Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ohmi
authors.affiliation: c Graduate School of Regional Innovation Studies , Mie University , Tsu, Japan.
authors.forename: Akiyo
authors.initials: A
authors.lastname: Sakamiya
authors.affiliation: c Graduate School of Regional Innovation Studies , Mie University , Tsu, Japan.
authors.forename: Takeo
authors.initials: T
authors.lastname: Yano
authors.affiliation: d Department of Life Sciences, Graduate School of Bioresources , Mie University , Tsu, Japan.
authors.forename: Katsuzumi
authors.initials: K
authors.lastname: Okumura
authors.affiliation: a Tsuji Health & Beauty Science Laboratory , Mie University , Tsu, Japan.|e Department of Translational Medical Sciences, Graduate School of Medicine , Mie University , Tsu, Japan.
authors.forename: Norihiro
authors.initials: N
authors.lastname: Nishimura
authors.affiliation: a Tsuji Health & Beauty Science Laboratory , Mie University , Tsu, Japan.|b Tsuji Oil Mills Co., Ltd. , Matsusaka , Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kagontani
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D020939:Zingiber officinale / Q000737:chemistry*; D006586:Hexanes / Q000737:chemistry*; D051379:Mice; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D053245:RANK Ligand / Q000502:physiology*
chemical_list: D006586:Hexanes; D010936:Plant Extracts; D053245:RANK Ligand; C505689:Tnfsf11 protein, mouse
keywords: RANKL; ginger; hexane extract; osteoclast; osteoporosis