title: Chikusetsusaponin V attenuates lipopolysaccharide-induced liver injury in mice.
abstract: Chikusetsusaponin V (CsV), a saponin from Panax japonicus, has been reported to inhibit inflammatory responses in lipopolysaccharide (LPS)-induced macrophage cells. However, whether CsV could alleviate LPS-induced liver injury in vivo and the potential mechanisms involved remain unclear. In the present study, we investigated the anti-inflammatory effects of CsV on LPS-induced acute liver injury in mice and further explored the potential mechanisms involved. Our results showed that CsV significantly attenuated elevation of alanine transaminase (ALT) and aspartate aminotransferase (AST) levels and improved liver histopathological changes in LPS-induced mice. In addition, CsV decreased serum tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) levels and inhibited mRNA expressions of inducible nitric oxide synthase (iNOS), TNF-α and IL-1β in LPS challenged mice. Furthermore, CsV inhibited nuclear factor kappa B (NF-κB) activation by downregulating phosphorylated NF-κB, IκB-α, ERK, c-Jun N-terminal kinase (JNK) and p38 levels in the liver tissue, which ultimately decreased nucleus NF-κB protein level. In conclusion, our data suggested that CsV could be a promising drug for preventing LPS challenged liver injury since it attenuated LPS-induced inflammatory responses, partly via inhibiting NF-κB and MAPK signaling pathways.
authors.affiliation: a Department of Pharmacology , China Three Gorges University , Yichang , Hubei , China ;
authors.forename: Yan Wen
authors.initials: YW
authors.lastname: Dai
authors.affiliation: a Department of Pharmacology , China Three Gorges University , Yichang , Hubei , China ;|b Third-Grade Pharmacological Laboratory on Chinese Medicine Approved by State Administration of Traditional Chinese Medicine , China Three Gorges University , Yichang , Hubei , China ;
authors.forename: Chang Cheng
authors.initials: CC
authors.lastname: Zhang
authors.affiliation: a Department of Pharmacology , China Three Gorges University , Yichang , Hubei , China ;|b Third-Grade Pharmacological Laboratory on Chinese Medicine Approved by State Administration of Traditional Chinese Medicine , China Three Gorges University , Yichang , Hubei , China ;
authors.forename: Hai Xia
authors.initials: HX
authors.lastname: Zhao
authors.affiliation: a Department of Pharmacology , China Three Gorges University , Yichang , Hubei , China ;
authors.forename: Jing Zhi
authors.initials: JZ
authors.lastname: Wan
authors.affiliation: a Department of Pharmacology , China Three Gorges University , Yichang , Hubei , China ;
authors.forename: Li Li
authors.initials: LL
authors.lastname: Deng
authors.affiliation: a Department of Pharmacology , China Three Gorges University , Yichang , Hubei , China ;|b Third-Grade Pharmacological Laboratory on Chinese Medicine Approved by State Administration of Traditional Chinese Medicine , China Three Gorges University , Yichang , Hubei , China ;
authors.forename: Zhi Yong
authors.initials: ZY
authors.lastname: Zhou
authors.affiliation: a Department of Pharmacology , China Three Gorges University , Yichang , Hubei , China ;|b Third-Grade Pharmacological Laboratory on Chinese Medicine Approved by State Administration of Traditional Chinese Medicine , China Three Gorges University , Yichang , Hubei , China ;
authors.forename: Yao Yan
authors.initials: YY
authors.lastname: Dun
authors.affiliation: c Department of Molecular Biology , College of Medical Science, China Three Gorges University , Yichang , Hubei , China ;
authors.forename: Chao Qi
authors.initials: CQ
authors.lastname: Liu
authors.affiliation: a Department of Pharmacology , China Three Gorges University , Yichang , Hubei , China ;|d Renhe Hospital, China Three Gorges University , Yichang , Hubei , China.
authors.forename: Ding
authors.initials: D
authors.lastname: Yuan
authors.affiliation: a Department of Pharmacology , China Three Gorges University , Yichang , Hubei , China ;|b Third-Grade Pharmacological Laboratory on Chinese Medicine Approved by State Administration of Traditional Chinese Medicine , China Three Gorges University , Yichang , Hubei , China ;
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
mesh_terms: D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000276:immunology / Q000517:prevention & control*; D053583:Interleukin-1beta / Q000276:immunology; D008070:Lipopolysaccharides / Q000633:toxicity*; D048670:MAP Kinase Kinase 4 / Q000276:immunology; D020935:MAP Kinase Signaling System / Q000187:drug effects* / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000276:immunology; D005894:Panax / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D048051:p38 Mitogen-Activated Protein Kinases / Q000276:immunology
chemical_list: C587672:IL1B protein, mouse; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D016328:NF-kappa B; D012503:Saponins; D014409:Tumor Necrosis Factor-alpha; C588229:chikusetsusaponin V; D048051:p38 Mitogen-Activated Protein Kinases; D048670:MAP Kinase Kinase 4
keywords: Chikusetsusaponin V; inflammatory; lipopolysaccharide; mitogen-activated protein kinase; nuclear factor kappa B