title: Enhanced Bone Repair by Guided Osteoblast Recruitment Using Topographically Defined Implant.
abstract: The rapid recruitment of osteoblasts in bone defects is an essential prerequisite for efficient bone repair. Conventionally, osteoblast recruitment to bone defects and subsequent bone repair has been achieved using growth factors. Here, we present a methodology that can guide the recruitment of osteoblasts to bone defects with topographically defined implants (TIs) for efficient in vivo bone repair. We compared circular TIs that had microgrooves in parallel or radial arrangements with nonpatterned implants for osteoblast migration and in vivo bone formation. In vitro, the microgrooves in the TIs enhanced both the migration and proliferation of osteoblasts. Especially, the microgrooves with radial arrangement demonstrated a much higher efficiency of osteoblast recruitment to the implants than did the other types of implants, which may be due to the efficient guidance of cell migration toward the cell-free area of the implants. The expression of the intracellular signaling molecules responsible for the cell migration was also upregulated in osteoblasts on the microgrooved TIs. In vivo, the TI with radially defined topography demonstrated much greater bone repair in mouse calvarial defect models than in the other types of implants. Taken together, these results indicate that implants with physical guidance can enhance tissue repair by rapid cell recruitment.
authors.affiliation: 1 School of Chemical and Biological Engineering, Seoul National University , Seoul, Republic of Korea.
authors.forename: Jeong-Kee
authors.initials: JK
authors.lastname: Yoon
authors.affiliation: 2 Center for BioMicrosystems, Brain Science Institute, Korea Institute of Science and Technology (KIST) , Seoul, Republic of Korea.|3 School of Mechanical and Aerospace Engineering, Seoul National University , Seoul, Republic of Korea.
authors.forename: Hong Nam
authors.initials: HN
authors.lastname: Kim
authors.affiliation: 4 School of Chemical Engineering, Sungkyunkwan University , Suwon, Republic of Korea.
authors.forename: Suk Ho
authors.initials: SH
authors.lastname: Bhang
authors.affiliation: 1 School of Chemical and Biological Engineering, Seoul National University , Seoul, Republic of Korea.
authors.forename: Jung-Youn
authors.initials: JY
authors.lastname: Shin
authors.affiliation: 1 School of Chemical and Biological Engineering, Seoul National University , Seoul, Republic of Korea.
authors.forename: Jin
authors.initials: J
authors.lastname: Han
authors.affiliation: 5 Department of Nanobiomedical Science, Dankook University , Chungnam, Republic of Korea.
authors.forename: Wan-Geun
authors.initials: WG
authors.lastname: La
authors.affiliation: 1 School of Chemical and Biological Engineering, Seoul National University , Seoul, Republic of Korea.
authors.forename: Gun-Jae
authors.initials: GJ
authors.lastname: Jeong
authors.affiliation: 1 School of Chemical and Biological Engineering, Seoul National University , Seoul, Republic of Korea.
authors.forename: Seokyung
authors.initials: S
authors.lastname: Kang
authors.affiliation: 1 School of Chemical and Biological Engineering, Seoul National University , Seoul, Republic of Korea.
authors.forename: Ju-Ro
authors.initials: JR
authors.lastname: Lee
authors.affiliation: 1 School of Chemical and Biological Engineering, Seoul National University , Seoul, Republic of Korea.
authors.forename: Jaesur
authors.initials: J
authors.lastname: Oh
authors.affiliation: 3 School of Mechanical and Aerospace Engineering, Seoul National University , Seoul, Republic of Korea.
authors.forename: Min Sung
authors.initials: MS
authors.lastname: Kim
authors.affiliation: 3 School of Mechanical and Aerospace Engineering, Seoul National University , Seoul, Republic of Korea.
authors.forename: Noo Li
authors.initials: NL
authors.lastname: Jeon
authors.affiliation: 1 School of Chemical and Biological Engineering, Seoul National University , Seoul, Republic of Korea.|6 Bio-MAX Institute, Institute of Chemical Processes, Seoul National University , Seoul, Republic of Korea.
authors.forename: Byung-Soo
authors.initials: BS
authors.lastname: Kim
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000473:pathology*; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology; D042541:Intracellular Space / Q000378:metabolism; D008813:Mice, Inbred ICR; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects; D019736:Prostheses and Implants; D015398:Signal Transduction / Q000187:drug effects; D014945:Wound Healing
chemical_list: D020099:Coated Materials, Biocompatible