title: Enzymatic hydrolysis of chitin pretreated by rapid depressurization from supercritical 1,1,1,2-tetrafluoroethane toward highly acetylated oligosaccharides.
abstract: The hydrolysis of chitin treated under supercritical conditions was successfully carried out using chitinases obtained by an optimized fermentation of the fungus Lecanicillium lecanii. The biopolymer was subjected to a pretreatment based on suspension in supercritical 1,1,1,2-tetrafluoroethane (scR134a), which possesses a critical temperature and pressure of 101°C and 40bar, respectively, followed by rapid depressurization to atmospheric pressure and further fibrillation. This methodology was compared to control untreated chitins and chitin subjected to steam explosion showing improved production of reducing sugars (0.18mg/mL), enzymatic hydrolysis and high acetylation (FA of 0.45) in products with degrees of polymerization between 2 and 5.
authors.affiliation: Universidad Autónoma Metropolitana, Biotechnology Department, Laboratory of Biopolymers and Pilot Plant of Bioprocessing of Agro-Industrial and Food By-Products, Av. San Rafael Atlixco No. 186, Col. Vicentina, C.P. 09340, Iztapalapa, México City, Mexico.
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Villa-Lerma
authors.affiliation: Universidad Autónoma Metropolitana, Biotechnology Department, Laboratory of Biopolymers and Pilot Plant of Bioprocessing of Agro-Industrial and Food By-Products, Av. San Rafael Atlixco No. 186, Col. Vicentina, C.P. 09340, Iztapalapa, México City, Mexico.
authors.forename: Humberto
authors.initials: H
authors.lastname: González-Márquez
authors.affiliation: Universidad Nacional Autónoma de México, Facultad de Química, Ciudad Universitaria, C.P. 04510, Mexico City, Mexico.
authors.forename: Miquel
authors.initials: M
authors.lastname: Gimeno
authors.affiliation: Ingénierie des Matériaux Polymères IMP@Lyon1, UMR CNRS 5223, Université Claude Bernard Lyon 1, Université de Lyon, 15 bd A. Latarjet, Villeurbanne Cedex, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Trombotto
authors.affiliation: Ingénierie des Matériaux Polymères IMP@Lyon1, UMR CNRS 5223, Université Claude Bernard Lyon 1, Université de Lyon, 15 bd A. Latarjet, Villeurbanne Cedex, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: David
authors.affiliation: Graduate School of Engineering, Department of Chemistry and Biotechnology, Tottori University, Koyamacho-minami 4-101, Tottori City, Tottori Prefecture 680-8550, Japan.
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Ifuku
authors.affiliation: Universidad Autónoma Metropolitana, Biotechnology Department, Laboratory of Biopolymers and Pilot Plant of Bioprocessing of Agro-Industrial and Food By-Products, Av. San Rafael Atlixco No. 186, Col. Vicentina, C.P. 09340, Iztapalapa, México City, Mexico. Electronic address: smk@xanum.uam.mx.
authors.forename: Keiko
authors.initials: K
authors.lastname: Shirai
mesh_terms: D000107:Acetylation; D002686:Chitin / Q000378:metabolism*; D002688:Chitinases / Q000737:chemistry* / Q000302:isolation & purification; D005285:Fermentation; D006845:Hydrocarbons, Fluorinated / Q000737:chemistry*; D006868:Hydrolysis; D006999:Hypocreales / Q000201:enzymology; D009844:Oligosaccharides / Q000737:chemistry*; D013227:Steam; D013696:Temperature; D004980:Ethane / Q000031:analogs & derivatives; D005466:Fluorocarbons
chemical_list: D002686:Chitin; D002688:Chitinases; D006845:Hydrocarbons, Fluorinated; D009844:Oligosaccharides; D013227:Steam; C063006:norflurane; D004980:Ethane; D005466:Fluorocarbons
keywords: Chitin; Chitinases; Lecanicillium lecanii; Supercritical 1,1,1,2-tetrafluoroethane; scR134a