title: Postnatal High-Fat Diet Increases Liver Steatosis and Apoptosis Threatened by Prenatal Dexamethasone through the Oxidative Effect.
abstract: The objective of this study was to investigate cellular apoptosis in prenatal glucocorticoid overexposure and a postnatal high fat diet in rats. Pregnant Sprague-Dawley rats at gestational days 14 to 21 were administered saline (vehicle) or dexamethasone and weaned onto either a normal fat diet or a high fat diet for 180 days; in total four experimental groups were designated, i.e., vehicle treated group (VEH), dexamethasone treated group (DEX), vehicle treated plus high-fat diet (VHF), and dexamethasone treated plus high-fat diet (DHF). Chronic effects of prenatal liver programming were assessed at postnatal day 180. The apoptotic pathways involved proteins were analyzed by Western blotting for their expressions. Apoptosis and liver steatosis were also examined by histology. We found that liver steatosis and apoptosis were increased in the DHF, DEX, and VHF treated groups, and that the DHF treated group was increased at higher levels than the DEX and VHF treated groups. The expression of leptin was decreased more in the DHF treated group than in the DEX and VHF treated groups. Decreased peroxisome proliferator-activated receptor-gamma coactivator 1α, phosphoinositide-3-kinase, manganese superoxide dismutase and increased malondialdehyde expression levels were seen in DHF treated group relative to the DEX treated group. The DHF treated group exhibited higher levels of oxidative stress, apoptosis and liver steatosis than the DEX treated group. These results indicate that the environment of high-fat diet plays an important role in the development of liver injury after prenatal stress.
authors.affiliation: Department of Pediatrics, College of Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University, No. 123, Ta-Pei road, Niaohsung, Kaohsiung 833, Taiwan. yhhuang123@yahoo.com.tw.
authors.forename: Ying-Hsien
authors.initials: YH
authors.lastname: Huang
authors.affiliation: Department of Pediatrics, College of Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University, No. 123, Ta-Pei road, Niaohsung, Kaohsiung 833, Taiwan. superarthy2@yahoo.com.
authors.forename: Chih-Jen
authors.initials: CJ
authors.lastname: Chen
authors.affiliation: Department of Pediatrics, College of Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University, No. 123, Ta-Pei road, Niaohsung, Kaohsiung 833, Taiwan. tangl004@cgmh.org.tw.
authors.forename: Kuo-Shu
authors.initials: KS
authors.lastname: Tang
authors.affiliation: Department of Pediatrics, College of Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University, No. 123, Ta-Pei road, Niaohsung, Kaohsiung 833, Taiwan. ray.sheen@gmail.com.
authors.forename: Jiunn-Ming
authors.initials: JM
authors.lastname: Sheen
authors.affiliation: Department of Pediatrics, College of Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University, No. 123, Ta-Pei road, Niaohsung, Kaohsiung 833, Taiwan. pc006581@yahoo.com.tw.
authors.forename: Mao-Meng
authors.initials: MM
authors.lastname: Tiao
authors.affiliation: Department of Pediatrics, College of Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University, No. 123, Ta-Pei road, Niaohsung, Kaohsiung 833, Taiwan. tainyl@adm.cgmh.com.tw.
authors.forename: You-Lin
authors.initials: YL
authors.lastname: Tain
authors.affiliation: Department of Pediatrics, College of Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University, No. 123, Ta-Pei road, Niaohsung, Kaohsiung 833, Taiwan. charllysc@cgmh.org.tw.
authors.forename: Chih-Cheng
authors.initials: CC
authors.lastname: Chen
authors.affiliation: Department of Pediatrics, College of Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University, No. 123, Ta-Pei road, Niaohsung, Kaohsiung 833, Taiwan. 3823212123456789@yahoo.com.tw.
authors.forename: En-Wei
authors.initials: EW
authors.lastname: Chu
authors.affiliation: Department of Pediatrics, College of Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University, No. 123, Ta-Pei road, Niaohsung, Kaohsiung 833, Taiwan. violet7053@gmail.com.
authors.forename: Shih-Wen
authors.initials: SW
authors.lastname: Li
authors.affiliation: Department of Pediatrics, College of Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University, No. 123, Ta-Pei road, Niaohsung, Kaohsiung 833, Taiwan. yuu2004taiwan@yahoo.com.tw.
authors.forename: Hong-Ren
authors.initials: HR
authors.lastname: Yu
authors.affiliation: Department of Pediatrics, College of Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University, No. 123, Ta-Pei road, Niaohsung, Kaohsiung 833, Taiwan. litung.huang@gmail.com.
authors.forename: Li-Tung
authors.initials: LT
authors.lastname: Huang
mesh_terms: D000818:Animals; D017209:Apoptosis; D003907:Dexamethasone / Q000009:adverse effects*; D059305:Diet, High-Fat / Q000009:adverse effects*; D005234:Fatty Liver / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D005260:Female; D020738:Leptin / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D018811:Maternal Exposure / Q000009:adverse effects*; D018384:Oxidative Stress; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D020738:Leptin; D003907:Dexamethasone
keywords: apoptosis; dexamethasone; high-fat diet; liver steatosis