title: High glucose concentration-induced expression of pentraxin-3 in a rat model of continuous peritoneal dialysis.
abstract: Continuous exposure to peritoneal dialysis fluids (PDFs) is associated with pathological responses such as persistent micro-inflammation, which leads to ultrafiltration failure. Pentraxin-3 (PTX3), a multifunctional soluble pattern recognition receptor, is produced at sites of inflammation by a wide range of cell types. This study investigates the in vivo expression of PTX3 in the peritoneal membrane of a rat continuous peritoneal dialysis (PD) model, as well as the effect of high glucose on the in vitro expression of PTX3.
  
  
  
  The expression of PTX3 was analyzed using RT-PCR, real-time PCR, immunohistochemistry and western blotting in a PD rat model receiving saline or conventional PDF containing 3.86% glucose for 8 weeks. The effects of high glucose on the expression of PTX3 were examined in cultured rat peritoneal mesothelial cells (RPMCs), mouse macrophage-like cells, and mouse fibroblasts.
  
  
  
  In a rat model of PD, eight-week instillation of the conventional PDF produced increased submesothelial thickening, followed by substantially enhanced PTX3 protein levels in the submesothelial layer of peritoneal membrane. PTX3 was detected in peritoneal mesothelial cells, macrophages and fibroblasts in the thickened submesothelial area. Glucose was found to induce PTX3 protein expression in RPMCs as well as macrophage-like cells and fibroblasts.
  
  
  
  Continuous exposure to conventional PDF induces PTX3 expression in the peritoneal membrane of rats. High glucose may be involved in the mechanism of PDF-induced local micro-inflammation in the peritoneum.
authors.affiliation: The Second Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, Iseigaoka, Yahatanishi, Kitakyushu, Japan.|Department of Anatomy, School of Medicine, University of Occupational and Environmental Health, Iseigaoka, Yahatanishi, Kitakyushu, Japan.
authors.forename: Nana
authors.initials: N
authors.lastname: Ishimatsu
authors.affiliation: The Second Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, Iseigaoka, Yahatanishi, Kitakyushu, Japan. tetsum@med.uoeh-u.ac.jp.
authors.forename: Tetsu
authors.initials: T
authors.lastname: Miyamoto
authors.affiliation: The Second Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, Iseigaoka, Yahatanishi, Kitakyushu, Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Ueno
authors.affiliation: The Second Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, Iseigaoka, Yahatanishi, Kitakyushu, Japan.
authors.forename: Emi
authors.initials: E
authors.lastname: Hasegawa
authors.affiliation: The Second Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, Iseigaoka, Yahatanishi, Kitakyushu, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Kuma
authors.affiliation: The Second Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, Iseigaoka, Yahatanishi, Kitakyushu, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Fujimoto
authors.affiliation: Kidney Center, School of Medicine, University of Occupational and Environmental Health, Iseigaoka, Yahatanishi, Kitakyushu, Japan.
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Bando
authors.affiliation: The Second Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, Iseigaoka, Yahatanishi, Kitakyushu, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Nakamata
authors.affiliation: The Second Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, Iseigaoka, Yahatanishi, Kitakyushu, Japan.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Furuno
authors.affiliation: Kidney Center, School of Medicine, University of Occupational and Environmental Health, Iseigaoka, Yahatanishi, Kitakyushu, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Serino
authors.affiliation: Department of Anatomy, School of Medicine, University of Occupational and Environmental Health, Iseigaoka, Yahatanishi, Kitakyushu, Japan.
authors.forename: Ryoko
authors.initials: R
authors.lastname: Baba
authors.affiliation: Department of Anatomy, School of Medicine, University of Occupational and Environmental Health, Iseigaoka, Yahatanishi, Kitakyushu, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Morimoto
authors.affiliation: Department of Anatomy, School of Medicine, University of Occupational and Environmental Health, Iseigaoka, Yahatanishi, Kitakyushu, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Doi
authors.affiliation: Kidney Center, School of Medicine, University of Occupational and Environmental Health, Iseigaoka, Yahatanishi, Kitakyushu, Japan.
authors.forename: Masahito
authors.initials: M
authors.lastname: Tamura
authors.affiliation: The Second Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, Iseigaoka, Yahatanishi, Kitakyushu, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Otsuji
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002097:C-Reactive Protein / Q000096:biosynthesis*; D015314:Dialysis Solutions / Q000737:chemistry*; D004195:Disease Models, Animal; D005947:Glucose / Q000008:administration & dosage*; D007150:Immunohistochemistry; D007249:Inflammation / Q000209:etiology; D010531:Peritoneal Dialysis, Continuous Ambulatory / Q000009:adverse effects* / Q000379:methods*; D010537:Peritoneum / Q000378:metabolism; D016133:Polymerase Chain Reaction; D051381:Rats; D017208:Rats, Wistar; D000683:Serum Amyloid P-Component / Q000096:biosynthesis*; D000099153:Pentraxins
chemical_list: D002097:C-Reactive Protein; D015314:Dialysis Solutions; D005947:Glucose; D000683:Serum Amyloid P-Component; D000099153:Pentraxins