title: The effect of metamizole on ischemia/reperfusion injury in the rat ovary: An analysis of biochemistry, molecular gene expression, and histopathology.
abstract: OBJECTIVE
  In this study, we investigated the effect of metamizole on ischemia/reperfusion (I/R) injury an analysis of biochemistry, molecular gene expression, and histopathology in the rat ovary of female albino Wistar rats.
  
  
  METHODS
  Animals were divided into four groups; control group with induced ischemia-reperfusion (IRC), ischemia-reperfusion +100 mg/kg metamizole sodium (MS) (IRM-100), ischemia-reperfusion +200 mg/kg MS (IRM-200), and healthy group applied sham operation (SG).
  
  
  RESULTS
  Myeloperoxidase (MPO) activity and gene expression increased significantly in IRC and IRM-100 group rat ovarian tissue compared with the SG group (P < 0.0001). However, MPO activity and gene expression in IRM-200 group ovarian tissue decreased significantly compared with the IRC and IRM-100 groups (P < 0.0001). Histopathologically, pronounced congestion, dilated vessels, hemorrhage, edema, degenerative cells, and neutrophil migration and adhesion to the endothelium were observed in the IRC and IRM-100 group ovarian tissues. A small number of congested dilated vessels, mild congestion, and edema were observed in the IRM-200 group, but no neutrophil migration and adhesion to the endothelium or degenerative cells.
  
  
  CONCLUSIONS
  At 200 mg/kg dose metamizole prevented ovarian injury induced with I/R. This data show that metamizole can be used in the ovarian I/R injury treatment.
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Sakarya University, Sakarya, Turkey.
authors.forename: Serkan
authors.initials: S
authors.lastname: Kumbasar
authors.affiliation: Department of Obstetrics and Gynecology, Gaziosmanpasa Taksim Education and Research Hospital, Istanbul, Turkey.
authors.forename: Suleyman
authors.initials: S
authors.lastname: Salman
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
authors.forename: Ragip Atakan
authors.initials: RA
authors.lastname: Al
authors.affiliation: Department of Anatomy, Erzurum Region Education and Research Hospital, Erzurum, Turkey.
authors.forename: Cengiz
authors.initials: C
authors.lastname: Ozturk
authors.affiliation: Department of Medical Genetics, İstanbul Medeniyet University Goztepe Training and Research Hospital, Istanbul, Turkey.
authors.forename: Oguzhan
authors.initials: O
authors.lastname: Yarali
authors.affiliation: Department of Biochemistry, Faculty of Medicine, 100. Yil University, Van, Turkey.
authors.forename: Hamit Hakan
authors.initials: HH
authors.lastname: Alp
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Erzincan University, Erzincan, Turkey.
authors.forename: Durdu
authors.initials: D
authors.lastname: Altuner
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Erzincan University, Erzincan, Turkey.
authors.forename: Bahadir
authors.initials: B
authors.lastname: Suleyman
mesh_terms: D000818:Animals; D004177:Dipyrone / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D010053:Ovary / Q000187:drug effects*; D009195:Peroxidase / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*
chemical_list: D004177:Dipyrone; D009195:Peroxidase
keywords: Gene expression; metamizole; myeloperoxidase; ovary; rat