title: Successful xenogeneic germ cell transplantation from Jundia catfish (Rhamdia quelen) into adult Nile tilapia (Oreochromis niloticus) testes.
abstract: Fish germ cell transplantation presents several important potential applications for aquaculture, including the preservation of germplasm from endangered fish species with high genetic and commercial values. Using this technique in studies developed in our laboratory with adult male Nile tilapias (Oreochromis niloticus), all the necessary procedures were successfully established, allowing the production of functional sperm and healthy progeny approximately 2months after allogeneic transplantation. In the present study, we evaluated the viability of the adult Nile tilapia testis to generate sperm after xenogeneic transplant of germ cells from sexually mature Jundia catfish (Rhamdia quelen) that belong to a different taxonomic order. Therefore, in order to investigate at different time-periods post-transplantation, the presence and development of donor PKH26 labeled catfish germ cells were followed in the tilapia seminiferous tubules. From 7 to 20days post-transplantation, only PKH26 labeled spermatogonia were observed, whereas spermatocytes at different stages of development were found at 70days. Germ cell transplantation success and progression of spermatogenesis were indicated by the presence of labeled PKH26 spermatids and sperm on days 90 and 120 post-transplantation, respectively. Confirming the presence of the catfish genetic material in the tilapia testis, all recipient tilapias evaluated (n=8) showed the genetic markers evaluated. Therefore, we demonstrated for the first time that the adult Nile tilapia testis offers the functional conditions for development of spermatogenesis with sperm production from a fish species belonging to a different order, which provides an important new venue for aquaculture advancement.
authors.affiliation: Laboratory of Cellular Biology (Dept. of Morphology), Federal University of Minas Gerais, MG 31270-901, Brazil.
authors.forename: M A
authors.initials: MA
authors.lastname: Silva
authors.affiliation: Laboratory of Cellular Biology (Dept. of Morphology), Federal University of Minas Gerais, MG 31270-901, Brazil.
authors.forename: G M J
authors.initials: GM
authors.lastname: Costa
authors.affiliation: Laboratory of Cellular Biology (Dept. of Morphology), Federal University of Minas Gerais, MG 31270-901, Brazil.
authors.forename: S M S N
authors.initials: SM
authors.lastname: Lacerda
authors.affiliation: Laboratory of Biotechnology and Molecular Markers (Dept. of General Biology), Federal University of Minas Gerais, MG 31270-901, Brazil.
authors.forename: P F P
authors.initials: PF
authors.lastname: Brandão-Dias
authors.affiliation: Laboratory of Biotechnology and Molecular Markers (Dept. of General Biology), Federal University of Minas Gerais, MG 31270-901, Brazil.
authors.forename: E
authors.initials: E
authors.lastname: Kalapothakis
authors.affiliation: Laboratory of Aquaculture (Veterinary School), Federal University of Minas Gerais, MG 31270-901, Brazil.
authors.forename: A F
authors.initials: AF
authors.lastname: Silva Júnior
authors.affiliation: Laboratory of Aquaculture (Veterinary School), Federal University of Minas Gerais, MG 31270-901, Brazil.
authors.forename: E R
authors.initials: ER
authors.lastname: Alvarenga
authors.affiliation: Laboratory of Cellular Biology (Dept. of Morphology), Federal University of Minas Gerais, MG 31270-901, Brazil; National Institute for Amazonian Research, Manaus, AM 69067-375, Brazil. Electronic address: lrfranca@icb.ufmg.br.
authors.forename: L R
authors.initials: LR
authors.lastname: França
mesh_terms: D000818:Animals; D017756:Aquaculture / Q000379:methods; D002397:Catfishes / Q000235:genetics / Q000378:metabolism*; D017690:Cell Transplantation; D003247:Conservation of Natural Resources / Q000379:methods; D056727:Endangered Species; D064593:Heterografts / Q000166:cytology* / Q000254:growth & development; D008297:Male; D012671:Seminiferous Tubules / Q000166:cytology; D013087:Spermatids / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D013090:Spermatocytes / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D013091:Spermatogenesis; D013093:Spermatogonia / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D013094:Spermatozoa / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D013737:Testis / Q000166:cytology* / Q000502:physiology; D017210:Tilapia / Q000235:genetics / Q000378:metabolism*; D014183:Transplantation, Heterologous
keywords: Jundia catfish (Rhamdia quelen); Nile-tilapia (Oreochromis niloticus); Spermatogenesis; Spermatogonial stem cells; Xenogeneic germ cells transplantation