title: Development and antigenic characterization of three recombinant proteins with potential for Glässer's disease prevention.
abstract: Haemophilus parasuis is the causative agent of Glässer's disease, which causes high morbidity and mortality in piglets, leading to severe economic losses. The lack of a commercial vaccine against a broad spectrum of strains has limited the disease control. H. parasuis outer membrane proteins (OMPs) are potentially essential components for vaccine formulation. In this study, seven putative OMPs were selected from the annotated H. parasuis serovar 5 genome; they were predicted by bioinformatics and annotated as potential virulence-related factors. These proteins were cloned, expressed, and purified as His-tagged proteins. Antigenicity of the candidate proteins was assessed using Western blotting with convalescent sera against H. parasuis serovar 5. The immunogenicity of the seven OMPs was assessed in a guinea pig model. Except VacJ, all the other six recombinant proteins elicited a detectable antibody response. Antisera against four of the selected proteins effectively killed the bacteria in vitro. Three proteins (Omp26, VacJ, and HAPS_0742) were found to confer signiﬁcant protection against challenge with a lethal dose of H. parasuis in a guinea pig model. The results suggest that these three proteins have a strong potential to be vaccine candidates against Glässer's disease.
authors.affiliation: Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong, China; Public Laboratory of Veterinary Public Health of Guangdong Province, Guangzhou 510640, Guangdong, China; Guangdong Key Laboratory of Animal Epidemic Diseases Prevention, Guangzhou 510640, Guangdong, China; College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, Guangdong, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Li
authors.affiliation: Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong, China; Public Laboratory of Veterinary Public Health of Guangdong Province, Guangzhou 510640, Guangdong, China; Guangdong Key Laboratory of Animal Epidemic Diseases Prevention, Guangzhou 510640, Guangdong, China. Electronic address: lclclare@163.com.
authors.forename: Chunling
authors.initials: C
authors.lastname: Li
authors.affiliation: Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong, China; Public Laboratory of Veterinary Public Health of Guangdong Province, Guangzhou 510640, Guangdong, China; Guangdong Key Laboratory of Animal Epidemic Diseases Prevention, Guangzhou 510640, Guangdong, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Song
authors.affiliation: Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong, China; Public Laboratory of Veterinary Public Health of Guangdong Province, Guangzhou 510640, Guangdong, China; Guangdong Key Laboratory of Animal Epidemic Diseases Prevention, Guangzhou 510640, Guangdong, China.
authors.forename: Huahua
authors.initials: H
authors.lastname: Kang
authors.affiliation: Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong, China; Public Laboratory of Veterinary Public Health of Guangdong Province, Guangzhou 510640, Guangdong, China; Guangdong Key Laboratory of Animal Epidemic Diseases Prevention, Guangzhou 510640, Guangdong, China.
authors.forename: Dongxia
authors.initials: D
authors.lastname: Yang
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, Guangdong, China. Electronic address: gqli@scau.edu.cn.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D005620:Freund's Adjuvant / Q000008:administration & dosage; D006168:Guinea Pigs; D006192:Haemophilus Infections / Q000517:prevention & control / Q000662:veterinary*; D018073:Haemophilus Vaccines / Q000276:immunology*; D044137:Haemophilus parasuis / Q000145:classification; D007106:Immune Sera / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D065288:Serogroup; D058248:Serum Bactericidal Antibody Assay; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D018073:Haemophilus Vaccines; D007106:Immune Sera; D011994:Recombinant Proteins; D005620:Freund's Adjuvant
keywords: Haemophilus parasuis; Immune responses; Outer membrane proteins; Protection; Vaccine candidate