title: Photocatalysis-Induced Renewable Field-Effect Transistor for Protein Detection.
abstract: The field-effect transistor (FET) biosensor has attracted extensive attentions, due to its unique features in detecting various biomolecules with high sensitivity and selectivity. However, currently used FET biosensors obtaining from expensive and elaborate micro/nanofabrication are always disposable because the analyte cannot be efficiently removed after detection. In this work, we established a photocatalysis-induced renewable graphene-FET (G-FET) biosensor for protein detection, by layer-to-layer assembling reduced graphene oxide (RGO) and RGO-encapsulated TiO2 composites to form a sandwiching RGO@TiO2 structure on a prefabricated FET sensor surface. After immobilization of anti-D-Dimer on the graphene surface, sensitive detection of D-Dimer was achieved with the detection limits of 10 pg/mL in PBS and 100 pg/mL in serum, respectively. Notably, renewal of the FET biosensor for recycling measurements was significantly realized by photocatalytically cleaning the substances on the graphene surface. This work demonstrates for the first time the development and application of photocatalytically renewable G-FET biosensor, paving a new way for G-FET sensor toward a plethora of diverse applications.
authors.affiliation: School of Laboratory Medicine, Hubei University of Chinese Medicine , 1 Huangjia Lake West Road, Wuhan 430065, P.R. China.
authors.forename: Changliang
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University ,Wuhan 430072, P.R. China.
authors.forename: Jia-Quan
authors.initials: JQ
authors.lastname: Xu
authors.affiliation: School of Laboratory Medicine, Hubei University of Chinese Medicine , 1 Huangjia Lake West Road, Wuhan 430065, P.R. China.
authors.forename: Yu-Tao
authors.initials: YT
authors.lastname: Li
authors.affiliation: Key Laboratory for the Physics and Chemistry of Nanodevices, Department of Electronics, Peking University , No. 5 Yiheyuan Road, Haidian District, Beijing 100871, P.R. China.
authors.forename: Le
authors.initials: L
authors.lastname: Huang
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University ,Wuhan 430072, P.R. China.
authors.forename: Dai-Wen
authors.initials: DW
authors.lastname: Pang
authors.affiliation: School of Laboratory Medicine, Hubei University of Chinese Medicine , 1 Huangjia Lake West Road, Wuhan 430065, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Ning
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University ,Wuhan 430072, P.R. China.
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Huang
authors.affiliation: Key Laboratory for the Physics and Chemistry of Nanodevices, Department of Electronics, Peking University , No. 5 Yiheyuan Road, Haidian District, Beijing 100871, P.R. China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: School of Laboratory Medicine, Hubei University of Chinese Medicine , 1 Huangjia Lake West Road, Wuhan 430065, P.R. China.
authors.forename: Guo-Jun
authors.initials: GJ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D002384:Catalysis; D002417:Cattle; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis*; D006801:Humans; D010316:Particle Size; D055668:Photochemical Processes; D012709:Serum Albumin / Q000032:analysis*; D013499:Surface Properties; D014173:Transistors, Electronic
chemical_list: D005338:Fibrin Fibrinogen Degradation Products; D012709:Serum Albumin; C036309:fibrin fragment D