title: Magnetic nanoparticles based dispersive micro-solid-phase extraction as a novel technique for the determination of estrogens in pork samples.
abstract: A simple and rapid magnetic nanoparticles (MNPs) based dispersive micro-solid-phase extraction (D-μ-SPE) method coupled with HPLC-DAD has been proposed for simultaneous determination of three estrogens (17β-estradiol (E2), estrone (E1) and diethylstilbestrol (DES)) in pork samples. In this paper, the synthesis of cetyltrimethyl ammonium bromide (CTAB)-coated Fe3O4@caprylic acid NPs as an efficient sorbent for its high surface area, excellent adsorption capacity, good dispersion ability and high super-paramagnetic property was successfully applied to adsorb estrogens. Vortex was used to enhance mass transfer rate as it provided mild and effective mixing of sample solution and increased the contact between analytes and MNPs. The parameters affecting the extraction efficiency were investigated in detail. The dosages of sorbent and eluate are 100μL and 500μL, respectively. The extraction equilibrium was achieved within 2min and the MNPs can be reused. The proposed technique provided high recoveries (93.3-106.7%), good linearity (0.9993-0.9999), low LODs (0.021-0.033ngmL(-1)) and repeatability (RSD%=1.87-2.92).
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Yunnan Province 650500, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Technology Centre of China Tobacco Guangxi Industrial Co., Ltd, Guangxi Nanning 530001, China.
authors.forename: Zhiyan
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Yunnan Jianniu Bio Technology Co., Ltd, Kunming 650033, China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Li
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Yunnan Province 650500, China. Electronic address: yilyil8@163.com.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000327:Adsorption; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000032:analysis / Q000302:isolation & purification*; D004967:Estrogens / Q000032:analysis / Q000302:isolation & purification*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008460:Meat / Q000032:analysis*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D013552:Swine
chemical_list: D004967:Estrogens; D058185:Magnetite Nanoparticles
keywords: 17β-Estradiol; CTAB; Diethylstilbestrol; Estrone; Fe(3)O(4)@caprylic acid NPs; HPLC; Magnetic nanoparticles based dispersive micro-solid-phase extraction