title: Enhanced production of erythritol and mannitol by Yarrowia lipolytica in media containing surfactants.
abstract: Various chemical compounds, including surfactants, when introduced to culture media may increase the permeability of cellular membranes and thereby affect the quantity of metabolites excreted by cells. The aim of the present study was to evaluate the impact of detergents including Triton X-100, Span 20 and Tween 80 on erythritol production from glycerol by Yarrowia lipolytica Wratislavia K1 in a shake-flask experiment, batch and fed-batch cultures. When Span 20 was added to a fed-batch culture with glycerol as a carbon source (300gL(-1)), erythritol production increased by 15% compared to the culture without the surfactant where it reached 142gL(-1) after 5 days, which corresponded to 0.47gg(-1) yield and productivity of 1.1gL(-1)h(-1). Therefore, it was concluded that Span 20 considerably enhanced the production of this polyol from glycerol.
authors.affiliation: Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences,Wrocław, Poland. Electronic address: magdalena.rakicka@up.wroc.pl.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Rakicka
authors.affiliation: Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences,Wrocław, Poland.
authors.forename: Anita
authors.initials: A
authors.lastname: Rywińska
authors.affiliation: Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences,Wrocław, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Cybulski
authors.affiliation: Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences,Wrocław, Poland.
authors.forename: Waldemar
authors.initials: W
authors.lastname: Rymowicz
mesh_terms: D003470:Culture Media / Q000737:chemistry / Q000378:metabolism*; D004896:Erythritol / Q000032:analysis / Q000096:biosynthesis*; D008353:Mannitol / Q000032:analysis / Q000378:metabolism*; D017830:Octoxynol / Q000032:analysis / Q000378:metabolism; D011136:Polysorbates / Q000032:analysis / Q000378:metabolism; D013501:Surface-Active Agents / Q000032:analysis / Q000378:metabolism*; D025062:Yarrowia / Q000378:metabolism*
chemical_list: D003470:Culture Media; D011136:Polysorbates; D013501:Surface-Active Agents; D008353:Mannitol; D017830:Octoxynol; D004896:Erythritol
keywords: Erythritol; Glycerol; Span 20; Surfactants; Yarrowia lipolytica