title: mRNA expression in different developmental stages of the chicken bursa of Fabricius.
abstract: The bursa of Fabricius, the central humoral immune organ unique to birds, plays an important role in B-lymphocyte differentiation. In order to gain a better understanding of the molecular mechanism of critical biological processes like B-cell immigration, differentiation, and final emigration, the transcriptional changes during embryonic and posthatch development of this organ were investigated. We generated a cDNA library from total RNA isolated from 3 representative developmental stages (embryonic day [ED] 10, posthatch d 2 and d 21). We generated over 70 million high-quality reads from the cDNA library by using deep sequencing. The uniquely mapped sequences of ED 10, d 2 and d 21 were 71087280, 59167491 and 70263675 respectively. All of the differential expressed genes were involved in Vitamin A metabolism, regulation of actin cytoskeleton, Wnt signaling pathway, MAPK signaling pathway, Jak-STAT signaling pathway, Notch signaling pathway, Toll-like receptor signaling pathway. The RNA-seq analysis provides a powerful method for analyzing the transcriptome and investigating the transcriptional changes of different development stages of bursa of Fabricius. The assembled bursa transcriptome provides an essential resource for future investigations about chicken Bursa development.
authors.affiliation: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China lxdau86@163.com xueqinzdxk888@163.com.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Liu
authors.affiliation: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Fubo
authors.initials: F
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China lxdau86@163.com xueqinzdxk888@163.com.
authors.forename: Hu
authors.initials: H
authors.lastname: Shan
authors.affiliation: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Shu-Bai
authors.initials: SB
authors.lastname: Wang
authors.affiliation: Division of Key Lab of Animal Disease Diagnosis and Immunology of China's Department of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Pu-Yan
authors.initials: PY
authors.lastname: Chen
mesh_terms: D000818:Animals; D002060:Bursa of Fabricius / Q000254:growth & development*; D002645:Chickens / Q000254:growth & development*; D015870:Gene Expression / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D063990:Gene Ontology; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D012333:RNA, Messenger
keywords: Bursa of Fabricius; RNA-seq; chicken