title: Rapid lateral-flow immunoassay for the quantum dot-based detection of puerarin.
abstract: In this study, a rapid (within 10min) quantitative lateral-flow immunoassay using a quantum dots (QDs)-antibody probe was developed for the analysis of puerarin (PUE) in water and biological samples. The competitive immunoassay was based on anti-PUE monoclonal antibody conjugated with QDs (detection reagent). Secondary antibody was immobilized on one end of a nitrocellulose membrane (control line) and PUE-bovine serum albumin conjugate was immobilized on the other end (test line). In the quantitative experiment, the detection results were scanned using a membrane strip reader and a detection curve (regression equation: y=-0.11ln(x)+0.979, R(2)=0.9816) representing the averages of the scanned data was obtained. This curve was linear from 1 to 10μg/mL. The IC50 value was 75.58ng/mL and the qualitative detection limit of PUE was 5.8ng/mL. The recovery of PUE added to phosphate-buffered saline and biological samples was in the range of 97.38-116.56%. To our knowledge, this is the first report of the quantitative detection of a natural product by QDs-based immunochromatography, which represents a powerful tool for rapidly screening PUE in plant materials and other biological samples.
authors.affiliation: Center of Scientific Experiment, Beijing University of Chinese Medicine, China.
authors.forename: Huihua
authors.initials: H
authors.lastname: Qu
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Basic Medical Sciences, Beijing University of Chinese Medicine, China.
authors.forename: Baoping
authors.initials: B
authors.lastname: Qu
authors.affiliation: School of Basic Medical Sciences, Beijing University of Chinese Medicine, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Kong
authors.affiliation: School of Basic Medical Sciences, Beijing University of Chinese Medicine, China.
authors.forename: Gaofeng
authors.initials: G
authors.lastname: Qin
authors.affiliation: School of Basic Medical Sciences, Beijing University of Chinese Medicine, China.
authors.forename: Shuchen
authors.initials: S
authors.lastname: Liu
authors.affiliation: School of Basic Medical Sciences, Beijing University of Chinese Medicine, China.
authors.forename: Jinjun
authors.initials: J
authors.lastname: Cheng
authors.affiliation: School of Basic Medical Sciences, Beijing University of Chinese Medicine, China. Electronic address: wangqg8558@sina.com.
authors.forename: Qingguo
authors.initials: Q
authors.lastname: Wang
authors.affiliation: School of Basic Medical Sciences, Beijing University of Chinese Medicine, China. Electronic address: zhaoyandr@gmail.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry*; D000911:Antibodies, Monoclonal / Q000737:chemistry; D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation; D002417:Cattle; D002846:Chromatography, Affinity / Q000191:economics / Q000295:instrumentation*; D004867:Equipment Design; D007887:Fabaceae / Q000737:chemistry*; D007529:Isoflavones / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D007529:Isoflavones; D010936:Plant Extracts; D012710:Serum Albumin, Bovine; C033607:puerarin
keywords: Lateral flow immunoassay; Monoclonal antibody; Puerarin; Quantum dot