title: In vitro and in cell analysis of chemically synthesized histone H2A with multiple modifications.
abstract: The chemical synthetic route to histone H2A is described. An H2A-H2B dimer, histone octamer, and nucleosome were reconstituted with the synthetic H2A. Fluorescein-labeled H2A and multiply modified H2A, which has three different posttranslational modifications, were also synthesized, and applied to live-cell imaging and in vitro nucleosome stability assays, respectively.
authors.affiliation: Department of Chemistry and Biotechnology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. okamoto@chembio.t.u-tokyo.ac.jp.
authors.forename: Gosuke
authors.initials: G
authors.lastname: Hayashi
authors.affiliation: Department of Chemistry and Biotechnology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. okamoto@chembio.t.u-tokyo.ac.jp.
authors.forename: Takuma
authors.initials: T
authors.lastname: Sueoka
authors.affiliation: Department of Chemistry and Biotechnology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. okamoto@chembio.t.u-tokyo.ac.jp and Address Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Okamoto