title: Biodiesel production from crude Jatropha oil catalyzed by non-commercial immobilized heterologous Rhizopus oryzae and Carica papaya lipases.
abstract: The aim of this study was to evaluate the feasibility of biodiesel production by transesterification of Jatropha oil with methanol, catalyzed by non-commercial sn-1,3-regioselective lipases. Using these lipases, fatty acid methyl esters (FAME) and monoacylglycerols are produced, avoiding the formation of glycerol as byproduct. Heterologous Rhizopus oryzae lipase (rROL) immobilized on different synthetic resins and Carica papaya lipase (rCPL) immobilized on Lewatit VP OC 1600 were tested. Reactions were performed at 30°C, with seven stepwise methanol additions. For all biocatalysts, 51-65% FAME (theoretical maximum=67%, w/w) was obtained after 4h transesterification. Stability tests were performed in 8 or 10 successive 4h-batches, either with or without rehydration of the biocatalyst between each two consecutive batches. Activity loss was much faster when biocatalysts were rehydrated. For rROL, half-life times varied from 16 to 579h. rROL on Lewatit VPOC 1600 was more stable than for rCPL on the same support.
authors.affiliation: Instituto Superior de Agronomia, Universidade de Lisboa, LEAF, Lisbon, Portugal.
authors.forename: J
authors.initials: J
authors.lastname: Rodrigues
authors.affiliation: Departament d'Enginyeria Quimica, Biològica i Ambiental (EE), Universitat Autònoma de Barcelona, Barcelona, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Canet
authors.affiliation: Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), Guadalajara, Jalisco, Mexico.
authors.forename: I
authors.initials: I
authors.lastname: Rivera
authors.affiliation: Instituto Superior de Agronomia, Universidade de Lisboa, LEAF, Lisbon, Portugal.
authors.forename: N M
authors.initials: NM
authors.lastname: Osório
authors.affiliation: Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), Guadalajara, Jalisco, Mexico.
authors.forename: G
authors.initials: G
authors.lastname: Sandoval
authors.affiliation: Departament d'Enginyeria Quimica, Biològica i Ambiental (EE), Universitat Autònoma de Barcelona, Barcelona, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Valero
authors.affiliation: Instituto Superior de Agronomia, Universidade de Lisboa, LEAF, Lisbon, Portugal. Electronic address: suzanafdias@mail.telepac.pt.
authors.forename: S
authors.initials: S
authors.lastname: Ferreira-Dias
mesh_terms: D056804:Biofuels; D001709:Biotechnology / Q000379:methods*; D029441:Carica / Q000201:enzymology*; D002384:Catalysis; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004951:Esterification; D004952:Esters / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D031286:Jatropha / Q000737:chemistry*; D008049:Lipase / Q000378:metabolism*; D008962:Models, Theoretical; D010578:Petroleum; D012233:Rhizopus / Q000201:enzymology*; D013997:Time Factors
chemical_list: D056804:Biofuels; D004800:Enzymes, Immobilized; D004952:Esters; D005227:Fatty Acids; D010578:Petroleum; D008049:Lipase
keywords: Biodiesel; Carica papaya lipase; Jatropha oil; Rhizopus oryzae lipase; sn-1,3 regioselective lipase