title: Membrane proteins involved in transport, vesicle traffic and Ca(2+) signaling increase in beetroots grown in saline soils.
abstract: By separating plasma membrane proteins according to their hydropathy from beetroots grown in saline soils, several proteins probably involved in salt tolerance were identified by mass spectrometry. Beetroots, as a salt-tolerant crop, have developed mechanisms to cope with stresses associated with saline soils. To observe which plasma membrane (PM) proteins were more abundant in beet roots grown in saline soils, beet root plants were irrigated with water or 0.2 M NaCl. PM-enriched membrane preparations were obtained from these plants, and their proteins were separated according to their hydropathy by serial phase partitioning with Triton X-114. Some proteins whose abundance increased visibly in membranes from salt-grown beetroots were identified by mass spectrometry. Among them, there was a V-type H(+)-ATPase (probably from contaminating vacuolar membranes), which increased with salt at all stages of beetroots' development. Proteins involved in solute transport (an H(+)-transporting PPase and annexins), vesicle traffic (clathrin and synaptotagmins), signal perception and transduction (protein kinases and phospholipases, mostly involved in calcium signaling) and metabolism, appeared to increase in salt-grown beetroot PM-enriched membranes. These results suggest that PM and vacuolar proteins involved in transport, metabolism and signal transduction increase in beet roots adapted to saline soils. In addition, these results show that serial phase partitioning with Triton X-114 is a useful method to separate membrane proteins for their identification by mass spectrometry.
authors.affiliation: Departamento de Biotecnología y Bioquímica, Unidad Irapuato, Cinvestav-IPN. Km 9.6 Libramiento Norte, 36821, Irapuato, Gto, Mexico.
authors.forename: Bárbara
authors.initials: B
authors.lastname: Lino
authors.affiliation: Departamento de Biotecnología y Bioquímica, Unidad Irapuato, Cinvestav-IPN. Km 9.6 Libramiento Norte, 36821, Irapuato, Gto, Mexico.
authors.forename: Alicia
authors.initials: A
authors.lastname: Chagolla
authors.affiliation: Departamento de Biotecnología y Bioquímica, Unidad Irapuato, Cinvestav-IPN. Km 9.6 Libramiento Norte, 36821, Irapuato, Gto, Mexico. lgonzale@ira.cinvestav.mx.
authors.forename: Luis
authors.initials: L
authors.lastname: E González de la Vara
mesh_terms: D027461:Beta vulgaris / Q000254:growth & development / Q000378:metabolism*; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D057927:Hydrophobic and Hydrophilic Interactions; D013058:Mass Spectrometry / Q000379:methods; D008565:Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism*; D012965:Sodium Chloride / Q000737:chemistry; D012987:Soil / Q000737:chemistry*; D022161:Transport Vesicles / Q000378:metabolism; D025262:Vacuolar Proton-Translocating ATPases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D010940:Plant Proteins; D012987:Soil; D012965:Sodium Chloride; D025262:Vacuolar Proton-Translocating ATPases; D002118:Calcium
keywords: Beta vulgaris; Mass spectrometry; Plasma membrane proteins; Salt tolerance; Triton X-114