title: Yolk Sac Macrophages, Fetal Liver, and Adult Monocytes Can Colonize an Empty Niche and Develop into Functional Tissue-Resident Macrophages.
abstract: Tissue-resident macrophages can derive from yolk sac macrophages (YS-Macs), fetal liver monocytes (FL-MOs), or adult bone-marrow monocytes (BM-MOs). The relative capacity of these precursors to colonize a niche, self-maintain, and perform tissue-specific functions is unknown. We simultaneously transferred traceable YS-Macs, FL-MOs, and BM-MOs into the empty alveolar macrophage (AM) niche of neonatal Csf2rb(-/-) mice. All subsets produced AMs, but in competition preferential outgrowth of FL-MOs was observed, correlating with their superior granulocyte macrophage-colony stimulating factor (GM-CSF) reactivity and proliferation capacity. When transferred separately, however, all precursors efficiently colonized the alveolar niche and generated AMs that were transcriptionally almost identical, self-maintained, and durably prevented alveolar proteinosis. Mature liver, peritoneal, or colon macrophages could not efficiently colonize the empty AM niche, whereas mature AMs could. Thus, precursor origin does not affect the development of functional self-maintaining tissue-resident macrophages and the plasticity of the mononuclear phagocyte system is largest at the precursor stage.
authors.affiliation: Unit of Immunoregulation and Mucosal Immunology, VIB Inflammation Research Center, Ghent 9052, Belgium; Department of Internal Medicine, Ghent University, Ghent 9000, Belgium.
authors.forename: Lianne
authors.initials: L
authors.lastname: van de Laar
authors.affiliation: Department of Internal Medicine, Ghent University, Ghent 9000, Belgium; Data Mining and Modeling for Systems Immunology, VIB Inflammation Research Center, Ghent 9052, Belgium.
authors.forename: Wouter
authors.initials: W
authors.lastname: Saelens
authors.affiliation: Unit of Immunoregulation and Mucosal Immunology, VIB Inflammation Research Center, Ghent 9052, Belgium; Department of Biomedical Molecular Biology, Ghent University, Ghent 9000, Belgium.
authors.forename: Sofie
authors.initials: S
authors.lastname: De Prijck
authors.affiliation: Department of Internal Medicine, Ghent University, Ghent 9000, Belgium; Data Mining and Modeling for Systems Immunology, VIB Inflammation Research Center, Ghent 9052, Belgium.
authors.forename: Liesbet
authors.initials: L
authors.lastname: Martens
authors.affiliation: Unit of Immunoregulation and Mucosal Immunology, VIB Inflammation Research Center, Ghent 9052, Belgium; Department of Biomedical Molecular Biology, Ghent University, Ghent 9000, Belgium.
authors.forename: Charlotte L
authors.initials: CL
authors.lastname: Scott
authors.affiliation: Unit of Immunoregulation and Mucosal Immunology, VIB Inflammation Research Center, Ghent 9052, Belgium; Department of Internal Medicine, Ghent University, Ghent 9000, Belgium.
authors.forename: Gert
authors.initials: G
authors.lastname: Van Isterdael
authors.affiliation: Department of Biomedical Molecular Biology, Ghent University, Ghent 9000, Belgium; Unit of Molecular Signal Transduction in Inflammation, VIB Inflammation Research Center, Ghent 9052, Belgium.
authors.forename: Eik
authors.initials: E
authors.lastname: Hoffmann
authors.affiliation: Department of Biomedical Molecular Biology, Ghent University, Ghent 9000, Belgium; Unit of Molecular Signal Transduction in Inflammation, VIB Inflammation Research Center, Ghent 9052, Belgium.
authors.forename: Rudi
authors.initials: R
authors.lastname: Beyaert
authors.affiliation: Department of Internal Medicine, Ghent University, Ghent 9000, Belgium; Data Mining and Modeling for Systems Immunology, VIB Inflammation Research Center, Ghent 9052, Belgium.
authors.forename: Yvan
authors.initials: Y
authors.lastname: Saeys
authors.affiliation: Unit of Immunoregulation and Mucosal Immunology, VIB Inflammation Research Center, Ghent 9052, Belgium; Department of Internal Medicine, Ghent University, Ghent 9000, Belgium; Pulmonary Medicine, Erasmus University Medical Center, Rotterdam 3015CE, the Netherlands.
authors.forename: Bart N
authors.initials: BN
authors.lastname: Lambrecht
authors.affiliation: Unit of Immunoregulation and Mucosal Immunology, VIB Inflammation Research Center, Ghent 9052, Belgium; Department of Biomedical Molecular Biology, Ghent University, Ghent 9000, Belgium. Electronic address: martin.guilliams@ugent.be.
authors.forename: Martin
authors.initials: M
authors.lastname: Guilliams
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000276:immunology*; D049109:Cell Proliferation; D053648:Cytokine Receptor Common beta Subunit / Q000235:genetics; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology*; D008099:Liver / Q000166:cytology* / Q000196:embryology / Q000276:immunology; D016676:Macrophages, Alveolar / Q000166:cytology* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D059467:Transcriptome / Q000276:immunology; D015017:Yolk Sac / Q000166:cytology* / Q000276:immunology
chemical_list: D053648:Cytokine Receptor Common beta Subunit; C072186:Csf2rb protein, mouse; D016178:Granulocyte-Macrophage Colony-Stimulating Factor