title: Production of nanotubes in delignified porous cellulosic materials after hydrolysis with cellulase.
abstract: In this study, tubular cellulose (TC), a porous cellulosic material produced by delignification of sawdust, was treated with a Trichoderma reesei cellulase in order to increase the proportion of nano-tubes. The effect of enzyme concentration and treatment duration on surface characteristics was studied and the samples were analyzed with BET, SEM and XRD. Also, a composite material of gelatinized starch and TC underwent enzymatic treatment in combination with amylase (320U) and cellulase (320U) enzymes. For TC, the optimum enzyme concentration (640U) led to significant increase of TC specific surface area and pore volume along with the reduction of pore diameter. It was also shown that the enzymatic treatment did not result to a significant change of cellulose crystallinity index. The produced nano-tubular cellulose shows potential for application to drug and chemical preservative delivery systems.
authors.affiliation: Food Biotechnology Group, Department of Chemistry, University of Patras, Patras 26500, Greece. Electronic address: a.a.koutinas@upatras.gr.
authors.forename: Αthanasios Α
authors.initials: ΑΑ
authors.lastname: Koutinas
authors.affiliation: Food Biotechnology Group, Department of Chemistry, University of Patras, Patras 26500, Greece.
authors.forename: Evgenia
authors.initials: E
authors.lastname: Papafotopoulou-Patrinou
authors.affiliation: Food Biotechnology Group, Department of Chemistry, University of Patras, Patras 26500, Greece.
authors.forename: Angelika-Ioanna
authors.initials: AI
authors.lastname: Gialleli
authors.affiliation: Food Biotechnology Group, Department of Chemistry, University of Patras, Patras 26500, Greece.
authors.forename: Theano
authors.initials: T
authors.lastname: Petsi
authors.affiliation: Food Biotechnology Group, Department of Chemistry, University of Patras, Patras 26500, Greece.
authors.forename: Argyro
authors.initials: A
authors.lastname: Bekatorou
authors.affiliation: Food Biotechnology Group, Department of Chemistry, University of Patras, Patras 26500, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Kanellaki
mesh_terms: D000681:Amylases / Q000737:chemistry; D002480:Cellulase / Q000737:chemistry* / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry*; D006868:Hydrolysis; D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology / Q000379:methods*; D043942:Nanotubes / Q000737:chemistry*; D016062:Porosity; D013213:Starch; D013499:Surface Properties; D014242:Trichoderma / Q000201:enzymology; D014961:X-Ray Diffraction
chemical_list: D002482:Cellulose; D013213:Starch; D000681:Amylases; D002480:Cellulase
keywords: Cellulase; Chemical delivery systems; Enzymatic treatment; Nano-tube; Tubular cellulose