title: Scaling-down antibody radiolabeling reactions with zirconium-89.
abstract: The most widely cited procedures for radiolabeling antibodies with zirconium-89 for immuno-PET require multi-milligram amounts of antibody which can be cost-prohibitive, particularly during the research and development process. We therefore sought to develop a reliable (89)Zr-radiolabeling procedure that provides high radiochemical yields at the microgram scale.
authors.affiliation: CR-UK/MRC Oxford Institute for Radiation Oncology, University of Oxford, Oxford, OX3 7LJ, UK.
authors.forename: James C
authors.initials: JC
authors.lastname: Knight
authors.affiliation: Wales Research & Diagnostic PET Imaging Centre (PETIC), Institute for Translation, Innovation, Methodology & Engagement (TIME), School of Medicine, Heath Park, Cardiff University, Cardiff, Wales, UK.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Paisey
authors.affiliation: Wales Research & Diagnostic PET Imaging Centre (PETIC), Institute for Translation, Innovation, Methodology & Engagement (TIME), School of Medicine, Heath Park, Cardiff University, Cardiff, Wales, UK.
authors.forename: Adam M
authors.initials: AM
authors.lastname: Dabkowski
authors.affiliation: CR-UK/MRC Oxford Institute for Radiation Oncology, University of Oxford, Oxford, OX3 7LJ, UK.
authors.forename: Cristina
authors.initials: C
authors.lastname: Marculescu
authors.affiliation: Institute of infection and immunity, School of Medicine, Heath Park, Cardiff University, Cardiff, Wales, UK.
authors.forename: Anwen S
authors.initials: AS
authors.lastname: Williams
authors.affiliation: Wales Research & Diagnostic PET Imaging Centre (PETIC), Institute for Translation, Innovation, Methodology & Engagement (TIME), School of Medicine, Heath Park, Cardiff University, Cardiff, Wales, UK.
authors.forename: Christopher
authors.initials: C
authors.lastname: Marshall
authors.affiliation: CR-UK/MRC Oxford Institute for Radiation Oncology, University of Oxford, Oxford, OX3 7LJ, UK.
authors.forename: Bart
authors.initials: B
authors.lastname: Cornelissen
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D018796:Immunoconjugates / Q000737:chemistry; D007553:Isotope Labeling / Q000379:methods*; D011868:Radioisotopes; D015040:Zirconium / Q000737:chemistry*
chemical_list: D000906:Antibodies; D018796:Immunoconjugates; D011868:Radioisotopes; D015040:Zirconium