title: A preliminary study for novel use of two Mg alloys (WE43 and Mg3Gd).
abstract: In this study, two types of magnesium alloys (WE43 and Mg3Gd) were compared with Heal-All membrane (a biodegradable membrane used in guided bone regeneration) in vitro to determine whether the alloys could be used as biodegradable membranes. Degradation behavior was assessed using immersion testing with simulated body fluid (SBF). Microstructural characteristics before and after immersion were evaluated through scanning electron microscopy, and degradation products were analyzed with energy dispersive spectrometry (EDS). To evaluate the biocompatibility of the three types of materials, we performed cytotoxicity, adhesion, and mineralization tests using human osteoblast-like MG63 cells. Immersion testing results showed no significant difference in degradation rate between WE43 and Mg3Gd alloys. However, both Mg alloys corroded faster than the Heal-All membrane, with pitting corrosion as the main corrosion mode for the alloys. Degradation products mainly included P- and Ca-containing apatites on the surface of WE43 and Mg3Gd, whereas these apatites were rarely detected on the surface of the Heal-All membrane. All three type of materials exhibited good biocompatibility. In the mineralization experiment, the alkaline phosphatase (ALP) activity of 10 % Mg3Gd extract was significantly higher than the extracts of the two other materials and the negative control. This study highlighted the potential of these Mg-REE alloys for uses in bone regeneration and further studies and refinements are obviously required.
authors.affiliation: Department of Dental Implantology, School and Hospital of Stomatology, Ji Lin University, Changchun, People's Republic of China.|Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Ji Lin University, Changchun, People's Republic of China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Dental Implantology, School and Hospital of Stomatology, Ji Lin University, Changchun, People's Republic of China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Ma
authors.affiliation: Department of Dental Implantology, School and Hospital of Stomatology, Ji Lin University, Changchun, People's Republic of China.
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Dental Implantology, School and Hospital of Stomatology, Ji Lin University, Changchun, People's Republic of China.
authors.forename: Jingting
authors.initials: J
authors.lastname: Zou
authors.affiliation: Department of Dental Implantology, School and Hospital of Stomatology, Ji Lin University, Changchun, People's Republic of China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Dental Implantology, School and Hospital of Stomatology, Ji Lin University, Changchun, People's Republic of China. zhaojh_1986@126.com.
authors.forename: Jinghui
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Dental Implantology, School and Hospital of Stomatology, Ji Lin University, Changchun, People's Republic of China. 2743416623@qq.com.
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000469:Alkaline Phosphatase; D000497:Alloys / Q000737:chemistry*; D001672:Biocompatible Materials; D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D006801:Humans; D017616:Magnesium Compounds / Q000737:chemistry*; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000502:physiology; D013314:Stress, Mechanical; D013499:Surface Properties
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; D017616:Magnesium Compounds; D008567:Membranes, Artificial; D000469:Alkaline Phosphatase