title: Longitudinal Frequencies of Blood Leukocyte Subpopulations Differ between NOD and NOR Mice but Do Not Predict Diabetes in NOD Mice.
abstract: Immune phenotyping provides insight into disease pathogenesis and prognostic markers. Trajectories from age of 4 to 36 weeks were modeled for insulin autoantibodies and for leukocyte subpopulations in peripheral blood from female NOD (n = 58) and NOR (n = 22) mice. NOD mice had higher trajectories of insulin autoantibodies, CD4(+) and CD8(+) T lymphocytes, B lymphocytes, IgD(+)IgM(-) B lymphocytes, and NK cells and lower trajectories of CD4(+)CD25(+) T lymphocytes, IgM(+) B lymphocytes, granulocytes, and monocytes than NOR mice (all p < 0.001). Of these, only the increased IAA trajectory was observed in NOD mice that developed diabetes as compared to NOD mice that remained diabetes-free. Therefore, the profound differences in peripheral blood leukocyte proportions observed between the diabetes-prone NOD mice and the diabetes-resistant mice do not explain the variation in diabetes development within NOD mice and do not provide markers for diabetes prediction in this model.
authors.affiliation: Institute for Diabetes and Obesity, Helmholtz Zentrum München, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany.
authors.forename: Tanja
authors.initials: T
authors.lastname: Telieps
authors.affiliation: Institute of Diabetes Research, Helmholtz Zentrum München and Forschergruppe Diabetes, Klinikum rechts der Isar, Technische Universität München, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany.
authors.forename: Meike
authors.initials: M
authors.lastname: Köhler
authors.affiliation: German Mouse Clinic, Institute of Experimental Genetics, Helmholtz Zentrum München, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany; Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Trogerstraße 30, 81675 Munich, Germany; German Center for Diabetes Research (DZD), Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany.
authors.forename: Irina
authors.initials: I
authors.lastname: Treise
authors.affiliation: Institute of Diabetes Research, Helmholtz Zentrum München and Forschergruppe Diabetes, Klinikum rechts der Isar, Technische Universität München, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany; Forschergruppe Diabetes e.V., Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Foertsch
authors.affiliation: German Mouse Clinic, Institute of Experimental Genetics, Helmholtz Zentrum München, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany; Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Trogerstraße 30, 81675 Munich, Germany; German Center for Diabetes Research (DZD), Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany.
authors.forename: Thure
authors.initials: T
authors.lastname: Adler
authors.affiliation: Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Trogerstraße 30, 81675 Munich, Germany.
authors.forename: Dirk H
authors.initials: DH
authors.lastname: Busch
authors.affiliation: German Mouse Clinic, Institute of Experimental Genetics, Helmholtz Zentrum München, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany; German Center for Diabetes Research (DZD), Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany; School of Life Science Weihenstephan, Technical University of Munich, Alte Akademie 8, 85354 Freising, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Hrabě de Angelis
authors.affiliation: Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Trogerstraße 30, 81675 Munich, Germany; Institute for Systemic Inflammation Research, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany.
authors.forename: Admar
authors.initials: A
authors.lastname: Verschoor
authors.affiliation: Institute of Diabetes Research, Helmholtz Zentrum München and Forschergruppe Diabetes, Klinikum rechts der Isar, Technische Universität München, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany; Forschergruppe Diabetes e.V., Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Adler
authors.affiliation: Institute for Diabetes and Obesity, Helmholtz Zentrum München, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany; German Center for Diabetes Research (DZD), Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany; Forschergruppe Diabetes e.V., Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany; DFG Research Center for Regenerative Therapies Dresden, Medical Faculty, Technische Universität Dresden, Fetscherstrasse 105, 01307 Dresden, Germany.
authors.forename: Ezio
authors.initials: E
authors.lastname: Bonifacio
authors.affiliation: Institute of Diabetes Research, Helmholtz Zentrum München and Forschergruppe Diabetes, Klinikum rechts der Isar, Technische Universität München, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany; German Center for Diabetes Research (DZD), Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany; Forschergruppe Diabetes e.V., Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany.
authors.forename: Anette-Gabriele
authors.initials: AG
authors.lastname: Ziegler
mesh_terms: D000367:Age Factors; D000818:Animals; D001323:Autoantibodies / Q000097:blood; D015415:Biomarkers / Q000097:blood; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000235:genetics / Q000276:immunology*; D005260:Female; D020022:Genetic Predisposition to Disease; D016130:Immunophenotyping; D007328:Insulin / Q000276:immunology; D007958:Leukocyte Count; D007962:Leukocytes / Q000276:immunology*; D016688:Mice, Inbred NOD; D010641:Phenotype; D013045:Species Specificity; D013997:Time Factors
chemical_list: D001323:Autoantibodies; D015415:Biomarkers; D007328:Insulin