title: High expression of Zinc-finger protein X-linked promotes tumor growth and predicts a poor outcome for stage II/III colorectal cancer patients.
abstract: Zinc-finger protein X-linked (ZFX) was recently identified as a novel oncoprotein in several human malignancies. In this study, we examined the correlation between ZFX expression and the clinical characteristics of stage II/III CRC patients, as well as the molecular mechanism by which ZFX apparently contributes to CRC tumor progression. Using immunohistochemistry, we detected expression of ZFX in CRC tissues collected from stage II/III patients and determined that its expression correlated with tumor differentiation and stage. Survival analysis indicated that patients with high ZFX expression had poorer overall and disease-free survival. ZFX knockdown in SW620 and SW480 CRC cells significantly inhibited cell proliferation and colony formation, enhanced apoptosis and induced cell cycle arrest. It also enhanced the sensitivity of CRC cells to 5-Fu. In a xenograft model, ZFX knockdown suppressed in vivo CRC tumor growth. Microarray analysis revealed the primary target of ZFX to be DUSP5. Whereas ZFX knockdown increased DUSP5 expression, DUSP5 knockdown rescued ZFX-mediated cell proliferation in ZFX knockdown cells. These findings demonstrate that ZFX promotes CRC progression by suppressing DUSP5 expression and suggest that ZFX is a novel prognostic biomarker and potentially useful therapeutic target in stage II/III CRC patients.
authors.affiliation: Department of General Surgery, The Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Xuebing
authors.initials: X
authors.lastname: Yan
authors.affiliation: Department of General Surgery, The Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Zezhi
authors.initials: Z
authors.lastname: Shan
authors.affiliation: Department of General Surgery, The Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Leilei
authors.initials: L
authors.lastname: Yan
authors.affiliation: Department of General Surgery, The Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Qingchao
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: Department of General Surgery, The Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Liguo
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Medicine, Soochow University, Suzhou, Jiangsu Province, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Xu
authors.affiliation: Department of Medicine, Soochow University, Suzhou, Jiangsu Province, China.
authors.forename: Sihong
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of General Surgery, The Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Jin
authors.affiliation: Center for Reproductive Medicine, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Gao
mesh_terms: D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D018938:Caco-2 Cells; D059447:Cell Cycle Checkpoints / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D018450:Disease Progression; D054637:Dual-Specificity Phosphatases / Q000235:genetics / Q000378:metabolism; D005472:Fluorouracil / Q000494:pharmacology; D020869:Gene Expression Profiling / Q000379:methods; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D007150:Immunohistochemistry; D051741:Kruppel-Like Transcription Factors / Q000096:biosynthesis* / Q000235:genetics*; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009367:Neoplasm Staging; D011379:Prognosis; D034622:RNA Interference; D014183:Transplantation, Heterologous
chemical_list: D000964:Antimetabolites, Antineoplastic; D051741:Kruppel-Like Transcription Factors; C062076:zinc finger protein, X-linked; C442320:DUSP5 protein, human; D054637:Dual-Specificity Phosphatases; D005472:Fluorouracil
keywords: DUSP5; ZFX; colorectal cancer; prognosis; tumor growth