title: Interleukin-13 is involved in the formation of liver fibrosis in Clonorchis sinensis-infected mice.
abstract: Clonorchiasis is a chronic infection disease often accompanied by formation of liver fibrosis. Previous study has identified that Clonorchis sinensis (C. sinensis, Cs) infection and CsRNASET2 (a member of CsESPs) immunization can drive Th2 immune response. IL-13, a multifunctional Th2 cytokine, has been widely confirmed to be profibrotic mediator. We want to determine whether IL-13 is involved in the generation of liver fibrosis during C. sinensis infection. A part of mice were infected with C. sinensis or immunized with CsRNASET2, respectively. Another part of mice were intravenously injected with rIL-13. Liver tissues of C. sinensis-infected mice were stained with hematoxylin-eosin and Masson's trichrome, respectively. The transcriptional levels of collagen-I, collagen-III, α-SMA, and TIMP-1 in the livers of infected mice and rIL-13-treated mice were measured by quantitative RT-PCR. Besides, splenocytes of C. sinensis-infected and CsRNASET2-immunized mice were isolated, respectively. The levels of IL-13 in splenocytes were detected by ELISA. Our results displayed that the livers of C. sinensis-infected mice had serious chronic inflammation and collagen deposition. The transcriptional levels of collagen-I, collagen-III, α-SMA, and TIMP-1 in the livers of C. sinensis-infected mice were obviously increased. Splenocytes from both C. sinensis-infected and CsRNASET2-immunized mice expressed high levels of IL-13. Moreover, rIL-13 treatment markedly promoted the transcriptional levels of collagen-I, collagen-III, α-SMA, and TIMP-1. These data implied that hepatic fibrosis was formed in the livers of C. sinensis-infected mice, and IL-13 induced by C. sinensis infection and CsRNASET2 immunization might favor this progression.
authors.affiliation: Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, People's Republic of China.
authors.forename: Yanquan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, People's Republic of China.|School of Tropical Medicine and Laboratory Medicine, Hainan Medical University, No. 3 Xueyuan Road, Haikou, Hainan, 571199, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Liang
authors.affiliation: Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, People's Republic of China.
authors.forename: Meng
authors.initials: M
authors.lastname: Bian
authors.affiliation: Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, People's Republic of China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, People's Republic of China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, People's Republic of China.
authors.forename: Jinsi
authors.initials: J
authors.lastname: Lin
authors.affiliation: Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, People's Republic of China.
authors.forename: Mei
authors.initials: M
authors.lastname: Shang
authors.affiliation: Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, People's Republic of China.
authors.forename: Hongling
authors.initials: H
authors.lastname: Qu
authors.affiliation: Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, People's Republic of China.
authors.forename: Zhongdao
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, People's Republic of China. huang66@mail.sysu.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, People's Republic of China. yuhxteam@163.com.
authors.forename: Xinbing
authors.initials: X
authors.lastname: Yu
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D003003:Clonorchiasis / Q000276:immunology* / Q000473:pathology; D003004:Clonorchis sinensis / Q000276:immunology; D003094:Collagen / Q000096:biosynthesis; D016207:Cytokines / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D018793:Interleukin-13 / Q000008:administration & dosage / Q000378:metabolism*; D008099:Liver / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D008103:Liver Cirrhosis / Q000469:parasitology* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000378:metabolism; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism
chemical_list: D000199:Actins; D016207:Cytokines; D018793:Interleukin-13; C560825:Timp1 protein, mouse; D019715:Tissue Inhibitor of Metalloproteinase-1; C544013:alpha-smooth muscle actin, mouse; D003094:Collagen
keywords: Clonorchis sinensis; Collagen; IL-13; Liver fibrosis