title: The Impact of TIMM17A on Aggressiveness of Human Breast Cancer Cells.
abstract: BACKGROUND
  The mitochondrial protein translocase of inner mitochondrial membrane 17 homolog A (TIMM17A) has been identified as a biomarker of breast cancer. The present study aimed to investigate the biological role of TIMM17A in human breast cancer cells.
  
  
  METHODS
  Anti-TIMM17A transgenes were stably transfected into MDA MB-231 and MCF-7 breast cancer cell lines. The impact of TIMM17A knock-down on cell migration and invasion were evaluated using the respective cell models.
  
  
  RESULTS
  Reducing the expression of TIMM17A in breast cancer cells resulted in reduction of cell migration using electric cell-substrate impedance sensing. It was also found that reduction of TIMM17A expression resulted in reduction of cell invasion compared to vector control.
  
  
  CONCLUSIONS
  TIMM17A has a profound impact on the cellular function of breast cancer cells. A decrease of TIMM17A expression is associated with the reduction of the aggressiveness of breast cancer cells. TIMM17A, therefore, has potential in prognosis and treatment of breast cancer.
authors.affiliation: Department of Biochemistry and Molecular Biology, Basic Medical School, Capital Medical University, Beijing, P.R. China Cancer Institute of Capital Medical University, Capital Medical University, Beijing, P.R. China Capital Medical University-Cardiff University Joint Centre For Biomedical Research, Capital Medical University, Beijing, P.R. China Beijing Key Laboratory for Cancer Invasion and Metastasis Research, Capital Medical University, Beijing, P.R. China Beijing International Cooperation Base for Science and Technology on China-UK Cancer Research, Capital Medical University, Beijing, P.R. China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Biochemistry and Molecular Biology, Basic Medical School, Capital Medical University, Beijing, P.R. China Cancer Institute of Capital Medical University, Capital Medical University, Beijing, P.R. China Capital Medical University-Cardiff University Joint Centre For Biomedical Research, Capital Medical University, Beijing, P.R. China Beijing Key Laboratory for Cancer Invasion and Metastasis Research, Capital Medical University, Beijing, P.R. China Beijing International Cooperation Base for Science and Technology on China-UK Cancer Research, Capital Medical University, Beijing, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Si
authors.affiliation: Department of Biochemistry and Molecular Biology, Basic Medical School, Capital Medical University, Beijing, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Tao
authors.affiliation: Metastasis & Angiogenesis Research Group, Cardiff China Medical Research Collaborative, Cardiff University School of Medicine, Cardiff, U.K.
authors.forename: Tracey A
authors.initials: TA
authors.lastname: Martin
authors.affiliation: Department of Biochemistry and Molecular Biology, Basic Medical School, Capital Medical University, Beijing, P.R. China Cancer Institute of Capital Medical University, Capital Medical University, Beijing, P.R. China Capital Medical University-Cardiff University Joint Centre For Biomedical Research, Capital Medical University, Beijing, P.R. China Beijing Key Laboratory for Cancer Invasion and Metastasis Research, Capital Medical University, Beijing, P.R. China Beijing International Cooperation Base for Science and Technology on China-UK Cancer Research, Capital Medical University, Beijing, P.R. China.
authors.forename: Shan
authors.initials: S
authors.lastname: Cheng
authors.affiliation: Department of Biochemistry and Molecular Biology, Basic Medical School, Capital Medical University, Beijing, P.R. China Cancer Institute of Capital Medical University, Capital Medical University, Beijing, P.R. China Capital Medical University-Cardiff University Joint Centre For Biomedical Research, Capital Medical University, Beijing, P.R. China Beijing Key Laboratory for Cancer Invasion and Metastasis Research, Capital Medical University, Beijing, P.R. China Beijing International Cooperation Base for Science and Technology on China-UK Cancer Research, Capital Medical University, Beijing, P.R. China.
authors.forename: Hefen
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Biochemistry and Molecular Biology, Basic Medical School, Capital Medical University, Beijing, P.R. China Cancer Institute of Capital Medical University, Capital Medical University, Beijing, P.R. China Capital Medical University-Cardiff University Joint Centre For Biomedical Research, Capital Medical University, Beijing, P.R. China Beijing Key Laboratory for Cancer Invasion and Metastasis Research, Capital Medical University, Beijing, P.R. China Beijing International Cooperation Base for Science and Technology on China-UK Cancer Research, Capital Medical University, Beijing, P.R. China.
authors.forename: Jinyao
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, Basic Medical School, Capital Medical University, Beijing, P.R. China Cancer Institute of Capital Medical University, Capital Medical University, Beijing, P.R. China Capital Medical University-Cardiff University Joint Centre For Biomedical Research, Capital Medical University, Beijing, P.R. China Beijing Key Laboratory for Cancer Invasion and Metastasis Research, Capital Medical University, Beijing, P.R. China Beijing International Cooperation Base for Science and Technology on China-UK Cancer Research, Capital Medical University, Beijing, P.R. China jq_he@ccmu.edu.cn JiangW@cardiff.ac.uk.
authors.forename: Junqi
authors.initials: J
authors.lastname: He
authors.affiliation: Beijing Key Laboratory for Cancer Invasion and Metastasis Research, Capital Medical University, Beijing, P.R. China Metastasis & Angiogenesis Research Group, Cardiff China Medical Research Collaborative, Cardiff University School of Medicine, Cardiff, U.K. jq_he@ccmu.edu.cn JiangW@cardiff.ac.uk.
authors.forename: Wen G
authors.initials: WG
authors.lastname: Jiang
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002465:Cell Movement; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D061986:MCF-7 Cells; D033681:Mitochondrial Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014162:Transfection
chemical_list: D033681:Mitochondrial Membrane Transport Proteins; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D012333:RNA, Messenger; C534731:TIMM17A protein, human
keywords: TIMM17A; human breast cancer; invasion; migration