title: The spatial-temporal expression and functional divergence of bach homologs in zebrafish Danio rerio.
abstract: The spatial-temporal expressions of bach1a, bach1b, bach2a and bach2b in early development of zebrafish Danio rerio embryos were examined in the present study by whole mount in situ hybridization. Transcripts of all genes were found at the one cell stage, suggesting that these four genes are maternally inherited. From the phylogenetic analyses, Bach1a and Bach1b from fishes form a well-supported group with two sub-groups. Bach2a and Bach2b, however, did not fall into one clade, suggesting that Bach2 proteins diverged faster or earlier than Bach1 proteins in fishes. The differentially regulated expression of the exocrine zymogen (such as the trypsin-like gene, tryl) of both paralogs of bach1 and bach2, respectively, showed that their functions are still active and already divergent, coinciding with subfunction partitioning.
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, P.R. China.
authors.forename: Q
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, P.R. China.
authors.forename: C
authors.initials: C
authors.lastname: Wu
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, P.R. China.
authors.forename: S
authors.initials: S
authors.lastname: Sun
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, P.R. China.
authors.forename: F
authors.initials: F
authors.lastname: Lu
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, P.R. China.
authors.forename: L
authors.initials: L
authors.lastname: Xie
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, P.R. China.
authors.forename: H
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, P.R. China.
authors.forename: X
authors.initials: X
authors.lastname: Zhong
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, P.R. China.
authors.forename: Q
authors.initials: Q
authors.lastname: Zhou
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D010802:Phylogeny; D020539:Sequence Analysis, Protein; D015027:Zebrafish / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics*
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; D029961:Zebrafish Proteins
keywords: bach; expression pattern; functional divergence; zebrafish