title: Fabrication, characterization, and biological assessment of multilayer laminin γ2 DNA coatings on titanium surfaces.
abstract: The purpose of this work was to fabricate a multilayer laminin γ2 DNA coating on a titanium surface and evaluate its biological properties. A multilayer laminin γ2 DNA coating was fabricated on titanium using a layer-by-layer assembly technique. The rate of coating degradation was evaluated by detecting the amount of cDNA remaining. Surface analysis using X-ray photoelectron spectroscopy, atomic force microscopy, and surface contact angle measurements revealed the multilayer structure to consist of cationic lipid and confirmed that a laminin γ2 DNA layer could be fabricated on titanium via the layer-by-layer assembly process. The transfection efficiency was highest for five layers in the multilayer structure. HEK293 cells cultured on the multilayer films displayed significantly higher adhesion activity than the control group. The expression of laminin γ2 and the co-localization of integrin β4 and plectin were more obvious in HN4 cells cultured on the multilayer laminin γ2 DNA coating, while weak immunoreactivities were observed in the control group. We concluded that the DNA-loaded multilayer provided a surface with good biocompatibility and that the multilayer laminin γ2 DNA coating might be effective in improving cell adhesion and the formation of hemidesmosomes on titanium surfaces.
authors.affiliation: Department of Implantology, Stomatology Hospital, School of Medical, Zhejiang University, Yan'an Road, Hangzhou, P. R. China.
authors.forename: Guoli
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of Implantology, Stomatology Hospital, School of Medical, Zhejiang University, Yan'an Road, Hangzhou, P. R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Implantology, Stomatology Hospital of Xuzhou, P. R. China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Dong
authors.affiliation: Department of Prosthodontics, Stomatology Hospital, School of Medical, Zhejiang University, Yan'an Road, Hangzhou, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Implantology, Stomatology Hospital, School of Medical, Zhejiang University, Yan'an Road, Hangzhou, P. R. China.
authors.forename: Jue
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Oral and Maxillofacial Surgery, Stomatology Hospital, School of Medical, Zhejiang University, Yan'an Road, Hangzhou, P. R. China.
authors.forename: Huiming
authors.initials: H
authors.lastname: Wang
mesh_terms: D002448:Cell Adhesion; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D018014:Gene Transfer Techniques; D057809:HEK293 Cells; D006801:Humans; D007797:Laminin / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D008422:Materials Testing; D056951:Photoelectron Spectroscopy; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology*; D014162:Transfection
chemical_list: D020099:Coated Materials, Biocompatible; C400567:LAMC2 protein, human; D007797:Laminin; D004247:DNA; D014025:Titanium