title: p53MutaGene: an online tool to estimate the effect of p53 mutational status on gene regulation in cancer.
abstract: p53MutaGene is the first online tool for statistical validation of hypotheses regarding the effect of p53 mutational status on gene regulation in cancer. This tool is based on several large-scale clinical gene expression data sets and currently covers breast, colon and lung cancers. The tool detects differential co-expression patterns in expression data between p53 mutated versus p53 normal samples for the user-specified genes. Statistically significant differential co-expression for a gene pair is indicative that regulation of two genes is sensitive to the presence of p53 mutations. p53MutaGene can be used in 'single mode' where the user can test a specific pair of genes or in 'discovery mode' designed for analysis of several genes. Using several examples, we demonstrate that p53MutaGene is a useful tool for fast statistical validation in clinical data of p53-dependent gene regulation patterns. The tool is freely available at http://www.bioprofiling.de/tp53.
authors.affiliation: Medical Research Council Toxicology Unit, Leicester LE1 9HN, UK.
authors.forename: I
authors.identifier: http://orcid.org/0000-0002-9126-5391
authors.initials: I
authors.lastname: Amelio
authors.affiliation: Medical Research Council Toxicology Unit, Leicester LE1 9HN, UK.
authors.forename: R A
authors.initials: RA
authors.lastname: Knight
authors.affiliation: Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: A
authors.initials: A
authors.lastname: Lisitsa
authors.affiliation: Medical Research Council Toxicology Unit, Leicester LE1 9HN, UK.|Department of Experimental Medicine & Surgery, University of Rome Tor Vergata, Rome 00133, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Melino
authors.affiliation: Medical Research Council Toxicology Unit, Leicester LE1 9HN, UK.
authors.forename: A V
authors.initials: AV
authors.lastname: Antonov
mesh_terms: D000818:Animals; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020407:Internet; D009154:Mutation; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism; D012984:Software; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53