title: DRACULA2 is a dynamic nucleoporin with a role in regulating the shade avoidance syndrome in Arabidopsis.
abstract: When plants grow in close proximity basic resources such as light can become limiting. Under such conditions plants respond to anticipate and/or adapt to the light shortage, a process known as the shade avoidance syndrome (SAS). Following genetic screening using a shade-responsive luciferase reporter line (PHYB:LUC), we identified DRACULA2 (DRA2), which encodes an Arabidopsis homolog of mammalian nucleoporin 98, a component of the nuclear pore complex (NPC). DRA2, together with other nucleoporins, participates positively in the control of the hypocotyl elongation response to plant proximity, a role that can be considered dependent on the nucleocytoplasmic transport of macromolecules (i.e. is transport dependent). In addition, our results reveal a specific role for DRA2 in controlling shade-induced gene expression. We suggest that this novel regulatory role of DRA2 is transport independent and that it might rely on its dynamic localization within and outside of the NPC. These results provide mechanistic insights in to how SAS responses are rapidly established by light conditions. They also indicate that nucleoporins have an active role in plant signaling.
authors.affiliation: Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB, 08193 Barcelona, Spain.
authors.forename: Marçal
authors.initials: M
authors.lastname: Gallemí
authors.affiliation: Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB, 08193 Barcelona, Spain.
authors.forename: Anahit
authors.initials: A
authors.lastname: Galstyan
authors.affiliation: Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB, 08193 Barcelona, Spain.
authors.forename: Sandi
authors.initials: S
authors.lastname: Paulišić
authors.affiliation: Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB, 08193 Barcelona, Spain.
authors.forename: Christiane
authors.initials: C
authors.lastname: Then
authors.affiliation: Instituto de Bioingeniería, Universidad Miguel Hernández, Campus de Elche, 03202 Elche, Spain.
authors.forename: Almudena
authors.initials: A
authors.lastname: Ferrández-Ayela
authors.affiliation: Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB, 08193 Barcelona, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Lorenzo-Orts
authors.affiliation: Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB, 08193 Barcelona, Spain.
authors.forename: Irma
authors.initials: I
authors.lastname: Roig-Villanova
authors.affiliation: School of Biological Sciences, Royal Holloway University of London, Egham TW20 0EX, UK.
authors.forename: Xuewen
authors.initials: X
authors.lastname: Wang
authors.affiliation: Instituto de Bioingeniería, Universidad Miguel Hernández, Campus de Elche, 03202 Elche, Spain.
authors.forename: Jose Luis
authors.initials: JL
authors.lastname: Micol
authors.affiliation: Instituto de Bioingeniería, Universidad Miguel Hernández, Campus de Elche, 03202 Elche, Spain.
authors.forename: Maria Rosa
authors.initials: MR
authors.lastname: Ponce
authors.affiliation: School of Biological Sciences, Royal Holloway University of London, Egham TW20 0EX, UK.
authors.forename: Paul F
authors.initials: PF
authors.lastname: Devlin
authors.affiliation: Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB, 08193 Barcelona, Spain Institució Catalana de Recerca i Estudis Avançats (ICREA), Ps. Lluís Companys 10, 08010 Barcelona, Spain jaume.martinez@cragenomica.es.
authors.forename: Jaime F
authors.initials: JF
authors.lastname: Martínez-García
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000235:genetics / Q000502:physiology*; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D018546:Hypocotyl / Q000235:genetics / Q000254:growth & development*; D008027:Light; D022022:Nuclear Pore / Q000235:genetics / Q000378:metabolism; D028861:Nuclear Pore Complex Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; C000622646:DRACULA2 protein, Arabidopsis; D028861:Nuclear Pore Complex Proteins; C093350:nuclear pore complex protein 98
keywords: Arabidopsis thaliana; Hypocotyl elongation; Nucleoporin; Nup98; Shade avoidance syndrome; Shade-induced gene expression