title: Biodegradable, Tethered Lipid Bilayer-Microsphere Systems with Membrane-Integrated α-Helical Peptide Anchors.
abstract: Supported lipid bilayers (SLBs) are ideally suited for the study of biomembrane-biomembrane interactions and for the biomimicry of cell-to-cell communication, allowing for surface ligand displays that contain laterally mobile elements. However, the SLB paradigm does not include three-dimensionality and biocompatibility. As a way to bypass these limitations, we have developed a biodegradable form of microsphere SLBs, also known as proteolipobeads (PLBs), using PLGA microspheres. Microspheres were synthesized using solvent evaporation and size selected with fluorescence activated cell sorting (FACS). Biomembranes were covalently tethered upon fusion to microsphere supports via short-chain PEG spacers connecting membrane-integrated α-helical peptides and the microsphere surface, affecting membrane diffusivity and mobility as indicated by confocal FRAP analysis. Membrane heterogeneities, which are attributed to PLGA hydrophobicity and rough surface topography, are curtailed by the addition of PEG tethers. This method allows for the presentation of tethered, laterally mobile biomembranes in three dimensions with functionally embedded attachment peptides for mobile ligand displays.
authors.affiliation: Department of Chemical Engineering and ‡Department of Biomedical Engineering, The City College of the City University of New York , 140th Street and Convent Avenue, New York, New York 10031, United States.
authors.forename: Eric S
authors.initials: ES
authors.lastname: Fried
authors.affiliation: Department of Chemical Engineering and ‡Department of Biomedical Engineering, The City College of the City University of New York , 140th Street and Convent Avenue, New York, New York 10031, United States.
authors.forename: Joshua
authors.initials: J
authors.lastname: Luchan
authors.affiliation: Department of Chemical Engineering and ‡Department of Biomedical Engineering, The City College of the City University of New York , 140th Street and Convent Avenue, New York, New York 10031, United States.
authors.forename: M Lane
authors.initials: ML
authors.lastname: Gilchrist
mesh_terms: D063566:Biodegradable Plastics / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry*; D008863:Microspheres; D010316:Particle Size; D010455:Peptides / Q000737:chemistry*; D010713:Phosphatidylcholines; D011092:Polyethylene Glycols / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D017433:Protein Structure, Secondary
chemical_list: D063566:Biodegradable Plastics; D008051:Lipid Bilayers; D008565:Membrane Proteins; D010455:Peptides; D010713:Phosphatidylcholines; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011092:Polyethylene Glycols; C017251:1,2-oleoylphosphatidylcholine