title: Biotechnological advancement in genetic improvement of broccoli (Brassica oleracea L. var. italica), an important vegetable crop.
abstract: With the advent of molecular biotechnology, plant genetic engineering techniques have opened an avenue for the genetic improvement of important vegetable crops. Vegetable crop productivity and quality are seriously affected by various biotic and abiotic stresses which destabilize rural economies in many countries. Moreover, absence of proper post-harvest storage and processing facilities leads to qualitative and quantitative losses. In the past four decades, conventional breeding has significantly contributed to the improvement of vegetable yields, quality, post-harvest life, and resistance to biotic and abiotic stresses. However, there are many constraints in conventional breeding, which can only be overcome by advancements made in modern biology. Broccoli (Brassica oleracea L. var. italica) is an important vegetable crop, of the family Brassicaceae; however, various biotic and abiotic stresses cause enormous crop yield losses during the commercial cultivation of broccoli. Thus, genetic engineering can be used as a tool to add specific characteristics to existing cultivars. However, a pre-requisite for transferring genes into plants is the availability of efficient regeneration and transformation techniques. Recent advances in plant genetic engineering provide an opportunity to improve broccoli in many aspects. The goal of this review is to summarize genetic transformation studies on broccoli to draw the attention of researchers and scientists for its further genetic advancement.
authors.affiliation: Department of Biotechnology, Dr Yashwant Singh Parmar University of Horticulture and Forestry, Solan, Himachal Pradesh, 173230, India. pksharmabiotech@gmail.com.
authors.forename: Pankaj
authors.identifier: http://orcid.org/0000-0002-2275-9144
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Department of Biotechnology, Dr Yashwant Singh Parmar University of Horticulture and Forestry, Solan, Himachal Pradesh, 173230, India.
authors.forename: Dinesh Kumar
authors.initials: DK
authors.lastname: Srivastava
mesh_terms: D001709:Biotechnology / Q000379:methods*; D001937:Brassica / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D018556:Crops, Agricultural / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D014675:Vegetables / Q000235:genetics / Q000378:metabolism / Q000502:physiology
keywords: Abiotic; Agrobacterium; Biotic resistance genes; Direct gene transfer; Genetic transformation; Plant biotechnology; Transgenic