title: Cyclic volatile methylsiloxanes in human blood as markers for ruptured silicone gel-filled breast implants.
abstract: The replacement of medical-grade silicone with industrial-grade silicone material in some silicone gel-filled breast implants (SBI) manufactured by Poly Implant Prothèse and Rofil Medical Nederland B.V., reported in 2010, which resulted in a higher rupture tendency of these SBI, demonstrates the need for non-invasive, sensitive monitoring and screening methods. Therefore a sensitive method based on large volume injection-gas chromatography coupled to mass spectrometry (LVI-GC/MS) was developed to determine octamethylcyclotetrasiloxane (D4), decamethylcyclopentasiloxane (D5), and dodecamethylcyclo-hexasiloxane (D6) in blood samples from women with intact (n = 13) and ruptured SBI (n = 11). With dichloromethane extraction, sample cooling during preparation, and analysis extraction efficiencies up to 100 % and limits of detection of 0.03-0.05 ng D4-D6/g blood were achieved. Blood samples from women with SBI were investigated. In contrast to women with intact SBI, in blood from women with ruptured SBI higher D4 and D6 concentrations up to 0.57 ng D4/g blood and 0.16 ng D6/g blood were detected. With concentrations above 0.18 D4 ng/blood and 0.10 ng D6/g blood as significant criteria for ruptured SBI, this developed analytical preoperative diagnostic method shows a significant increase of the recognition rate. Finally a higher precision (error rate 17%) than the commonly used clinical diagnostic method, mamma sonography (error rate 46%), was achieved.
authors.affiliation: Applied Analytical Chemistry, University of Duisburg-Essen, Universitaetsstr. 5, 45141, Essen, Germany.
authors.forename: Pia
authors.initials: P
authors.lastname: Rosendahl
authors.affiliation: Applied Analytical Chemistry, University of Duisburg-Essen, Universitaetsstr. 5, 45141, Essen, Germany.
authors.forename: Joerg
authors.initials: J
authors.lastname: Hippler
authors.affiliation: Applied Analytical Chemistry, University of Duisburg-Essen, Universitaetsstr. 5, 45141, Essen, Germany. oliver.schmitz@uni-due.de.
authors.forename: Oliver J
authors.initials: OJ
authors.lastname: Schmitz
authors.affiliation: Department of Gynecology and Obstetrics, School of Medicine, University of Duisburg-Essen, Hufelandstraße 55, 45147, Essen, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Hoffmann
authors.affiliation: Department of Gynecology and Obstetrics, School of Medicine, University of Duisburg-Essen, Hufelandstraße 55, 45147, Essen, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Rusch
mesh_terms: D001769:Blood; D018427:Breast Implants; D006801:Humans; D011786:Quality Control; D020034:Silicone Gels; D012833:Siloxanes / Q000737:chemistry*
chemical_list: D020034:Silicone Gels; D012833:Siloxanes
keywords: GC/MS; Human blood; Large volume injection; Silicone breast implants; Siloxanes