title: Importance of glucokinase -258G/A polymorphism in Asian Indians with post-transplant and type 2 diabetes mellitus.
abstract: Type 2 diabetes mellitus (T2DM) and post-transplant diabetes mellitus (PTDM) are non-synonymous forms of diabetes. Glucokinase (GCK) plays a key role in glucose metabolism. The relationship between the GCK promoter and specific types of diabetes, such as PTDM and T2DM, in the Asian Indian population is unknown. We examined the occurrence of a specific GCK promoter variant (-258G/A) in patients with T2DM and PTDM. The case-control study enrolled 640 Asian Indian subjects, including controls (n = 250) and T2DM (n = 250), PTDM (n = 42), and non-post-transplant diabetes mellitus (non-PTDM) (n = 98) patients. Purified Deoxyribonucleic acid (DNA) was genotyped with the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis. The digested PCR products were analyzed on 12% polyacrylamide gels. The anthropometric, biochemical, and clinical details of each group were documented. GCK -258G/A alleles and genotypes were not associated with T2DM. However, among PTDM subjects, we detected a higher frequency of heterozygotes (52.4%) and a positive association with alleles/genotypes. The results suggest that the promoter region (-258G/A) of GCK plays an important role in PTDM in Asian Indians.
authors.affiliation: Department of Genetics and Molecular medicine, Kamineni Hospitals, Hyderabad, India; Department of Genetics, Vasavi Medical and Research Centre, Khairathabad, Hyderabad, India; Department of Genetics and Biotechnology, Osmania University, Tarnaka, Hyderabad, India.
authors.forename: Imran Ali
authors.initials: IA
authors.lastname: Khan
authors.affiliation: Department of Genetics and Molecular medicine, Kamineni Hospitals, Hyderabad, India.
authors.forename: Kiran Kumar
authors.initials: KK
authors.lastname: Vattam
authors.affiliation: Department of Genetics and Biotechnology, Osmania University, Tarnaka, Hyderabad, India.
authors.forename: Parveen
authors.initials: P
authors.lastname: Jahan
authors.affiliation: Department of Genetics and Molecular medicine, Kamineni Hospitals, Hyderabad, India; Department of Genetics, Vasavi Medical and Research Centre, Khairathabad, Hyderabad, India.
authors.forename: Qurratulain
authors.initials: Q
authors.lastname: Hasan
authors.affiliation: Department of Biochemistry, Kasturba Medical College, Manipal University, Manipal, Karnataka, India.
authors.forename: Pragna
authors.initials: P
authors.lastname: Rao
keywords: Asian Indians; GCK; PTDM; T2DM; −258G/A