title: Production of poly-3-hydroxybutyrate (P3HB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV) from synthetic wastewater using Hydrogenophaga palleronii.
abstract: In the present study, synthetic wastewater (SW) was used for production of poly-3-hydroxybutyrate (P3HB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV) using the bacteria Hydrogenophaga palleronii. SW at various volatile fatty acids concentrations (5-60g/l) was evaluated for the growth and biopolymer production using H. palleronii. Substrate degradation was analyzed using total organic carbon (TOC) analyzer and high pressure liquid chromatography (HPLC). H. palleronii showed highest and lowest removal of TOC at 5g/l (88±4%) and 60g/l (15±6%) respectively. Among all the concentrations evaluated, bacteria showed highest biopolymer production with 20g/l (63±5%), followed by 30g/l (58±3%) and 40g/l (56±2%). Lowest biopolymer production was observed at 5g/l concentration (21±3%). Structure, molecular weight, and thermal properties of the produced biopolymer were analyzed. These results denoted that the strain H. palleronii can be used for degradation of high concentration of volatile fatty acids persistent in wastewaters and their subsequent conversion into useable biopolymers.
authors.affiliation: Department of Applied Sciences, College of Environmental Technology, Muroran Institute of Technology, 27-1 Mizumoto, Muroran, Hokkaido 050-8585, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Venkateswar Reddy
authors.affiliation: Research Center for Environmentally Friendly Materials Engineering, Muroran Institute of Technology, 27-1 Mizumoto-cho, Muroran, Hokkaido 050-8585, Japan.
authors.forename: Yasuteru
authors.initials: Y
authors.lastname: Mawatari
authors.affiliation: Department of Applied Sciences, College of Environmental Technology, Muroran Institute of Technology, 27-1 Mizumoto, Muroran, Hokkaido 050-8585, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Yajima
authors.affiliation: Department of Information and Electronic Engineering, Muroran Institute of Technology, 27-1 Mizumoto-cho, Muroran, Hokkaido 050-8585, Japan.
authors.forename: Kohki
authors.initials: K
authors.lastname: Satoh
authors.affiliation: Bioengineering and Environmental Sciences (BEES), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500007, India.
authors.forename: S
authors.initials: S
authors.lastname: Venkata Mohan
authors.affiliation: Department of Applied Sciences, College of Environmental Technology, Muroran Institute of Technology, 27-1 Mizumoto, Muroran, Hokkaido 050-8585, Japan. Electronic address: ychang@mmm.muroran-it.ac.jp.
authors.forename: Young-Cheol
authors.initials: YC
authors.lastname: Chang
mesh_terms: D042621:Comamonadaceae / Q000378:metabolism*; D005232:Fatty Acids, Volatile / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006885:Hydroxybutyrates / Q000378:metabolism*; D008970:Molecular Weight; D010421:Pentanoic Acids / Q000378:metabolism; D011091:Polyesters / Q000378:metabolism*; D062065:Wastewater / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification; D000094122:Polyhydroxybutyrates
chemical_list: D005232:Fatty Acids, Volatile; D006885:Hydroxybutyrates; D010421:Pentanoic Acids; D011091:Polyesters; D062065:Wastewater; D014874:Water Pollutants, Chemical; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C013056:beta-hydroxyvaleric acid; C003182:poly-beta-hydroxybutyrate
keywords: Biopolymer; Gel permeation chromatography; Hydrogenophaga palleronii; Sodium acetate; Total organic carbon