title: The C-terminal region of the transcriptional regulator THAP11 forms a parallel coiled-coil domain involved in protein dimerization.
abstract: Thanatos associated protein 11 (THAP11) is a cell cycle and cell growth regulator differentially expressed in cancer cells. THAP11 belongs to a distinct family of transcription factors recognizing specific DNA sequences via an atypical zinc finger motif and regulating diverse cellular processes. Outside the extensively characterized DNA-binding domain, THAP proteins vary in size and predicted domains, for which structural data are still lacking. We report here the crystal structure of the C-terminal region of human THAP11 protein, providing the first 3D structure of a coiled-coil motif from a THAP family member. We further investigate the stability, dynamics and oligomeric properties of the determined structure combining molecular dynamics simulations and biophysical experiments. Our results show that the C-ter region of THAP11 forms a left-handed parallel homo-dimeric coiled-coil structure possessing several unusual features.
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, UPS, France. Electronic address: cyprian.cukier@gmx.com.
authors.forename: Cyprian D
authors.initials: CD
authors.lastname: Cukier
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, UPS, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Maveyraud
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, UPS, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Saurel
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, UPS, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Guillet
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, UPS, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Milon
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, UPS, France. Electronic address: virginie.gervais@ipbs.fr.
authors.forename: Virginie
authors.initials: V
authors.lastname: Gervais
mesh_terms: D018360:Crystallography, X-Ray; D006801:Humans; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D000072417:Protein Domains / Q000502:physiology; D055503:Protein Multimerization; D055550:Protein Stability; D017433:Protein Structure, Secondary; D012097:Repressor Proteins / Q000737:chemistry* / Q000502:physiology
chemical_list: D012097:Repressor Proteins; C498470:THAP11 protein, human
keywords: Coiled-coil; Crystal structure; Molecular dynamics; NMR; Oligomerization; THAP11