title: Dual effect of LPS on murine myeloid leukemia cells: Pro-proliferation and anti-proliferation.
abstract: Modification of the bone marrow microenvironment is considered as a promising strategy to control leukemic cell proliferation, diseases progression and relapse after treatment. However, due to the diversity and complexity of the cellular and molecular compartments in the leukemic microenvironment, it is extremely difficult to dissect the role of each individual molecule or cell type in vivo. Here we established an in vitro system to dissect the role of lipopolysaccharide (LPS), stromal cells and endothelial cells in the growth of mouse myeloid tumor cells and B-lymphoma cells. We found that either LPS or bone marrow stromal cells as a feeder layer in culture is required for the proliferation of myeloid tumor cells. Surprisingly, the growth of myeloid leukemic cells on stromal cells is strongly inhibited when coupled with LPS in culture. This opposing effect of LPS, a complete switch from pro-proliferation to antitumor growth is due, at least in part, to the rapidly increased production of interleukin 12, Fas ligand and tissue inhibitor of metalloproteinases-2 from stromal cells stimulated by LPS. These results demonstrate that LPS can either facilitate or attenuate tumor cell proliferation, thus changing the disease course of myeloid leukemias through its direct effect or modulation of the tumor microenvironment.
authors.affiliation: Department of Pediatrics, Jingjiang People's Hospital, Yangzhou University, Jingjiang 214500, China; Noncoding RNA Center, Yangzhou University, Yangzhou 225001, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Pediatrics, Jingjiang People's Hospital, Yangzhou University, Jingjiang 214500, China.
authors.forename: Yingmin
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Noncoding RNA Center, Yangzhou University, Yangzhou 225001, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Gu
authors.affiliation: Noncoding RNA Center, Yangzhou University, Yangzhou 225001, China.
authors.forename: Jijun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Noncoding RNA Center, Yangzhou University, Yangzhou 225001, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Pang
authors.affiliation: Noncoding RNA Center, Yangzhou University, Yangzhou 225001, China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Noncoding RNA Center, Yangzhou University, Yangzhou 225001, China.
authors.forename: Yaoyao
authors.initials: Y
authors.lastname: Li
authors.affiliation: Noncoding RNA Center, Yangzhou University, Yangzhou 225001, China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Jia
authors.affiliation: Noncoding RNA Center, Yangzhou University, Yangzhou 225001, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Hematology, Yangzhou University School of Clinical Medicine, Yangzhou 225001, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Gu
authors.affiliation: Department of Pediatrics, Jingjiang People's Hospital, Yangzhou University, Jingjiang 214500, China; Noncoding RNA Center, Yangzhou University, Yangzhou 225001, China; Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Disease, Yangzhou 225001, China; Institute of Comparative Medicine, Yangzhou University, Yangzhou 225001, China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou 225001, China. Electronic address: duonan@yahoo.com.
authors.forename: Duonan
authors.initials: D
authors.lastname: Yu
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D019610:Cytoprotection / Q000187:drug effects; D007951:Leukemia, Myeloid / Q000473:pathology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D012995:Solubility; D017154:Stromal Cells / Q000166:cytology* / Q000187:drug effects
chemical_list: D008070:Lipopolysaccharides
keywords: Cytokines; Endothelial cells; Lipopolysaccharides; Myeloid leukemia; Stromal cells; Tumor microenvironment