title: Imidazolium-tagged glycan probes for non-covalent labeling of live cells.
abstract: Selective, bioorthogonal and fast labeling of glycoconjugates in living cells is a major challenge for synthetic and cellular biology. Here we report the use imidazolium tagged-mannosamine derivative (ITag-Man) for the non-covalent, rapid and site-specific labeling of sialic acid containing glycoproteins using commercial N-nitrilotriacetate fluorescent reagents in a range of cell lines.
authors.affiliation: School of Chemistry, University of Bristol, Cantock's Close, BS8 1TS, UK. M.C.Galan@bristol.ac.uk.
authors.forename: David
authors.initials: D
authors.lastname: Benito-Alifonso
authors.forename: Shirley
authors.initials: S
authors.lastname: Tremell
authors.forename: Joanna C
authors.initials: JC
authors.lastname: Sadler
authors.forename: Monica
authors.initials: M
authors.lastname: Berry
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Galan
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D007093:Imidazoles / Q000737:chemistry*; D015335:Molecular Probes / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D007093:Imidazoles; D015335:Molecular Probes; D011134:Polysaccharides