title: Functional second harmonic generation microscopy probes molecular dynamics with high temporal resolution.
abstract: Second harmonic generation (SHG) microscopy is a powerful tool for label free ex vivo or in vivo imaging, widely used to investigate structure and organization of endogenous SHG emitting proteins such as myosin or collagen. Polarization resolved SHG microscopy renders supplementary information and is used to probe different molecular states. This development towards functional SHG microscopy is calling for new methods for high speed functional imaging of dynamic processes. In this work we present two approaches with linear polarized light and demonstrate high speed line scan measurements of the molecular dynamics of the motor protein myosin with a time resolution of 1 ms in mammalian muscle cells. Such a high speed functional SHG microscopy has high potential to deliver new insights into structural and temporal molecular dynamics under ex vivo or in vivo conditions.
authors.affiliation: Medical Biophysics Unit, Institute for Physiology and Pathophysiology, University of Heidelberg, Heidelberg, Germany; m.foerderer@physiologie.uni-heidelberg.de.
authors.forename: Moritz
authors.initials: M
authors.lastname: Förderer
authors.affiliation: Medical Biophysics Unit, Institute for Physiology and Pathophysiology, University of Heidelberg, Heidelberg, Germany.
authors.forename: Tihomir
authors.initials: T
authors.lastname: Georgiev
authors.affiliation: Medical Biophysics Unit, Institute for Physiology and Pathophysiology, University of Heidelberg, Heidelberg, Germany.
authors.forename: Matias
authors.initials: M
authors.lastname: Mosqueira
authors.affiliation: Medical Biophysics Unit, Institute for Physiology and Pathophysiology, University of Heidelberg, Heidelberg, Germany.
authors.forename: Rainer H A
authors.initials: RH
authors.lastname: Fink
authors.affiliation: Medical Biophysics Unit, Institute for Physiology and Pathophysiology, University of Heidelberg, Heidelberg, Germany; Current address: Max Planck Institute of Biophysics, Frankfurt/Main, Germany; martin.vogel@biophys.mpg.de.
authors.forename: Martin
authors.initials: M
authors.lastname: Vogel
keywords: (170.2655) Functional monitoring and imaging; (180.4315) Nonlinear microscopy; (190.2620) Harmonic generation and mixing