title: Generation of iPSC lines from primary human amniotic fluid cells.
abstract: By means of retroviral transduction using the four Yamanaka-factors OCT4, SOX2, KLF4 and c-MYC primary human amniotic fluid cells (AFCs) were reprogrammed into several iPSC lines. Pluripotency was confirmed both in vitro and in vivo. A comparative transcriptome analysis of the AF-derived iPSC line 41 and the human embryonic stem cell lines (H1 and H9) revealed a Pearson correlation of 0.953 and 0.941 respectively.
authors.affiliation: Max Planck Institute for Molecular Genetics, 14195, Berlin, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Drews
authors.affiliation: Max Planck Institute for Molecular Genetics, 14195, Berlin, Germany; Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany.
authors.forename: Peggy
authors.initials: P
authors.lastname: Matz
authors.affiliation: Max Planck Institute for Molecular Genetics, 14195, Berlin, Germany; Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany. Electronic address: James.Adjaye@med.uni-duesseldorf.de.
authors.forename: James
authors.initials: J
authors.lastname: Adjaye
mesh_terms: D000653:Amniotic Fluid / Q000378:metabolism*; D002454:Cell Differentiation; D065150:Cellular Reprogramming; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism*; D000090062:Kruppel-Like Factor 4; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism*
chemical_list: C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D016271:Proto-Oncogene Proteins c-myc