title: Transcriptome response to copper heavy metal stress in hard-shelled mussel (Mytilus coruscus).
abstract: The hard-shelled mussel (Mytilus coruscus) has considerably one of the most economically important marine shellfish worldwide and considered as a good invertebrate model for ecotoxicity study for a long time. In the present study, we used Illumina sequencing technology (HiSeq2000) to sequence, assemble and annotate the transcriptome of the hard-shelled mussel which challenged with copper pollution. A total of 21,723,913 paired-end clean reads (NCBI SRA database SRX1411195) were generated from HiSeq2000 sequencer and 96,403 contigs (with N50 = 1118 bp) were obtained after de novo assembling with Trinity software. Digital gene expression analysis reveals 1156 unigenes are upregulated and 1681 unigenes are downregulated when challenged with copper. By KEGG pathway enrichment analysis, we found that unigenes in four KEGG pathways (aminoacyl-tRNA biosynthesis, apoptosis, DNA replication and mismatch repair) show significant differential expressed between control and copper treated groups. We hope that the gill transcriptome in copper treated hard-shelled mussel can give useful information to understand how mussel handles with heavy metal stress at molecular level.
authors.affiliation: The Key Laboratory of Molecular Biophysics of Ministry of Education, Huazhong University of Science and Technology (HUST), Luoyo Road 1037, Wuhan 430074, China; National Engineering Research Center of Marine Facilities Aquaculture, College of Marine Science, Zhejiang Ocean University, Zhoushan 316022, China.
authors.forename: Meiying
authors.initials: M
authors.lastname: Xu
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, College of Marine Science, Zhejiang Ocean University, Zhoushan 316022, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung 20224, Taiwan.
authors.forename: Kang-Ning
authors.initials: KN
authors.lastname: Shen
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, College of Marine Science, Zhejiang Ocean University, Zhoushan 316022, China.
authors.forename: Changwen
authors.initials: C
authors.lastname: Wu
authors.affiliation: The Key Laboratory of Molecular Biophysics of Ministry of Education, Huazhong University of Science and Technology (HUST), Luoyo Road 1037, Wuhan 430074, China.
authors.forename: Guangyuan
authors.initials: G
authors.lastname: He
authors.affiliation: Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li 32023, Taiwan.
authors.forename: Chung-Der
authors.initials: CD
authors.lastname: Hsiao
keywords: Ecotoxicity; Hard-shelled mussel; Heavy metal; Transcriptome