title: Specific effects of c-Jun NH2-terminal kinase-interacting protein 1 in neuronal axons.
abstract: c-Jun NH2-terminal kinase (JNK)-interacting protein 3 plays an important role in brain-derived neurotrophic factor/tropomyosin-related kinase B (TrkB) anterograde axonal transport. It remains unclear whether JNK-interacting protein 1 mediates similar effects, or whether JNK-interacting protein 1 affects the regulation of TrkB anterograde axonal transport. In this study, we isolated rat embryonic hippocampus and cultured hippocampal neurons in vitro. Coimmunoprecipitation results demonstrated that JNK-interacting protein 1 formed TrkB complexes in vitro and in vivo. Immunocytochemistry results showed that when JNK-interacting protein 1 was highly expressed, the distribution of TrkB gradually increased in axon terminals. However, the distribution of TrkB reduced in axon terminals after knocking out JNK-interacting protein 1. In addition, there were differences in distribution of TrkB after JNK-interacting protein 1 was knocked out compared with not. However, knockout of JNK-interacting protein 1 did not affect the distribution of TrkB in dendrites. These findings confirm that JNK-interacting protein 1 can interact with TrkB in neuronal cells, and can regulate the transport of TrkB in axons, but not in dendrites.
authors.affiliation: Department of Pharmacy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China.
authors.forename: Shu
authors.identifier: 0000-0002-3268-692X
authors.initials: S
authors.lastname: Tang
authors.affiliation: Department of Clinical Pharmacology, Basic Medical College, Zhengzhou University, Zhengzhou, Henan Province, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wen
authors.affiliation: Department of Pharmacy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China.
authors.forename: Xiao-Jian
authors.initials: XJ
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China.
authors.forename: Quan-Cheng
authors.initials: QC
authors.lastname: Kan
keywords: axons; brain-derived neurotrophic factor; c-Jun NH2-terminal kinase-interacting protein; dendrites; hippocampus; nerve regeneration; neural regeneration; neurons; regulation; tropomyosin-related kinase B