title: [Optimization of Ethanol Extraction and Water Extraction Technologies of Zuogui Pills by Orthogonal Test].
abstract: OBJECTIVE
  To optimize the ethanol extraction and water extraction technologies of Luogui Pills.
  
  
  METHODS
  An orthogonal test was used to study the influence of ethanol concentration, amount of ethanol and reflux time on hyperoside in Cuscutae Semen, ursolic acid in Corni Fructus and the yield of dry paste. Also, The effect of soaking time, amount of water and extraction time on acteoside in Rehmanniae Radix Praeparata and the yield of dry paste were investigated. All these factors were adopted as the indices for choosing the best ethanol extraction and water extraction technologies.
  
  
  RESULTS
  The best ethanol extraction technology for Cuscutae Semen, Corni Fructus and Achyranthis Bidentatae Radix was reflux extracting for 1 h each time for two times with eight times of 70% ethanol. The best water extraction technology for the residue and the rest herbs after the ethanol extraction was soaking for 0. 5 h and then decocting for 0. 5 h each time for two times with twelve times of water.
  
  
  CONCLUSIONS
  The optimal extraction technologies are stable and feasible, the extraction rate of the alcohol soluble and water soluble effective components is high, and it provides the reference basis for the secondary development of Zuogui Pills.
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.forename: Tong
authors.initials: T
authors.lastname: Zhang
authors.forename: Ben-liang
authors.initials: BL
authors.lastname: Xu
authors.forename: Xiu-rong
authors.initials: XR
authors.lastname: Yuan
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D000431:Ethanol; D005960:Glucosides; D010636:Phenols; D010936:Plant Extracts / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*; D014867:Water; D059808:Polyphenols
chemical_list: D004365:Drugs, Chinese Herbal; D000431:Ethanol; D005960:Glucosides; D010636:Phenols; D010936:Plant Extracts; D014867:Water; C552040:zuogui; C058956:acteoside; D059808:Polyphenols