title: Neuropeptidergic Signaling and Active Feeding State Inhibit Nociception in Caenorhabditis elegans.
abstract: Food availability and nutritional status are important cues affecting behavioral states. Here we report that, in Caenorhabditis elegans, a cascade of dopamine and neuropeptide signaling acts to inhibit nociception in food-poor environments. In the absence of food, animals show decreased sensitivity and increased adaptation to soluble repellents sensed by the polymodal ASH nociceptors. The effects of food on adaptation are affected by dopamine and neuropeptide signaling; dopamine acts via the DOP-1 receptor to decrease adaptation on food, whereas the neuropeptide receptors NPR-1 and NPR-2 act to increase adaptation off food. NPR-1 and NPR-2 function cell autonomously in the ASH neurons to increase adaptation off food, whereas the DOP-1 receptor controls neuropeptide release from interneurons that modulate ASH activity indirectly. These results indicate that feeding state modulates nociception through the interaction of monoamine and neuropeptide signaling pathways.
authors.affiliation: Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, United Kingdom, Department of Biosciences and Nutrition, Karolinska Institute, Hälsovägen 7, S-141 83 Huddinge, Sweden, and Institute of Healthy Ageing, and Research Department of Genetics, Evolution, and Environment, University College London, London WC1E 6BT, United Kingdom.
authors.forename: Marina
authors.identifier: http://orcid.org/0000-0003-2739-6984
authors.initials: M
authors.lastname: Ezcurra
authors.affiliation: Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, United Kingdom.
authors.forename: Denise S
authors.identifier: http://orcid.org/0000-0003-1534-1679
authors.initials: DS
authors.lastname: Walker
authors.affiliation: Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, United Kingdom.
authors.forename: Isabel
authors.initials: I
authors.lastname: Beets
authors.affiliation: Department of Biosciences and Nutrition, Karolinska Institute, Hälsovägen 7, S-141 83 Huddinge, Sweden, and.
authors.forename: Peter
authors.identifier: http://orcid.org/0000-0001-6416-8572
authors.initials: P
authors.lastname: Swoboda
authors.affiliation: Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, United Kingdom, wschafer@mrc-lmb.cam.ac.uk.
authors.forename: William R
authors.initials: WR
authors.lastname: Schafer
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D000818:Animals; D030801:Animals, Genetically Modified; D016466:CHO Cells; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism; D027202:Cationic Amino Acid Transporter 2 / Q000235:genetics / Q000378:metabolism; D003300:Copper / Q000494:pharmacology; D003412:Cricetulus; D004298:Dopamine / Q000235:genetics / Q000378:metabolism; D005215:Fasting; D005247:Feeding Behavior / Q000502:physiology*; D009479:Neuropeptides / Q000378:metabolism*; D059225:Nociception / Q000187:drug effects / Q000502:physiology*; D011401:Promoter Regions, Genetic / Q000235:genetics; D017447:Receptors, Dopamine D1 / Q000235:genetics / Q000378:metabolism; D017476:Receptors, Neuropeptide Y / Q000235:genetics / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000187:drug effects / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics / Q000502:physiology*
chemical_list: D029742:Caenorhabditis elegans Proteins; D002135:Calcium-Binding Proteins; D027202:Cationic Amino Acid Transporter 2; C488450:Dop-1 protein, C elegans; C468272:NPR-1 protein, C elegans; D009479:Neuropeptides; D017447:Receptors, Dopamine D1; D017476:Receptors, Neuropeptide Y; C107581:UNC-31 protein, C elegans; D003300:Copper; C029892:cupric chloride; D004298:Dopamine
keywords: C. elegans; chemosensation; dopamine; neuromodulation; neuropeptide