title: [Effect of radio frequency discharge plasma on surface properties and biocompatibility of polycaprolactone matrices].
abstract: Surface modification of bioresorbable polymer material (polycaprolactone, PCL) with abnormal glow discharge, initiated during radio-frequency magnetron sputtering of a hydroxyapatite target was investigated. Plasma treatment resulted in an increase of surface roughness of PCL, crystallite size, the surface free energy and hydrophilicity. Increased treatment time (30, 60, 150 seconds) provoked the polymer surface saturation with the sputtering target ions (calcium, phosphorus). The assessment of plasma exposure of PCL surface on bone marrow multipotent mesenchymal stromal cells behavior (BM MSCs) has been performed. Modification of the polymer surface with the abnormal glow discharge stimulated adhesion and subsequent proliferation of BM MSCs; thus, maximum values were achieved with the surface treatment for 60 s. This type of plasma modification did not affect cell viability (apoptosis, necrosis). Thus, the surface modification with abnormal glow discharge, initiated during radio-frequency magnetron sputtering of a hydroxyapatite target, appear to be a promising method of surface modification of bioresorbable polymer material (PCL) for tissue engineering.
authors.affiliation: National Research Tomsk Polytechnic University, Tomsk, Russia.
authors.forename: E N
authors.initials: EN
authors.lastname: Bolbasov
authors.affiliation: Research Institute for Complex Issues of Cardiovascular Disease, Kemerovo, Russia.
authors.forename: L V
authors.initials: LV
authors.lastname: Antonova
authors.affiliation: Research Institute for Complex Issues of Cardiovascular Disease, Kemerovo, Russia.
authors.forename: V G
authors.initials: VG
authors.lastname: Matveeva
authors.affiliation: National Research Tomsk State University, Tomsk, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Novikov
authors.affiliation: National Research Tomsk Polytechnic University, Tomsk, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Shesterikov
authors.affiliation: National Research Tomsk Polytechnic University, Tomsk, Russia.
authors.forename: N L
authors.initials: NL
authors.lastname: Bogomolova
authors.affiliation: Research Institute for Complex Issues of Cardiovascular Disease, Kemerovo, Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Golovkin
authors.affiliation: National Research Tomsk Polytechnic University, Tomsk, Russia.
authors.forename: S I
authors.initials: SI
authors.lastname: Tverdohlebov
authors.affiliation: Research Institute for Complex Issues of Cardiovascular Disease, Kemerovo, Russia.
authors.forename: O L
authors.initials: OL
authors.lastname: Barbarash
authors.affiliation: Research Institute for Complex Issues of Cardiovascular Disease, Kemerovo, Russia.
authors.forename: L S
authors.initials: LS
authors.lastname: Barbarash
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism*; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D058626:Plasma Gases / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013499:Surface Properties
chemical_list: D058626:Plasma Gases; D011091:Polyesters; C016240:polycaprolactone
keywords: biocompatibility; free energy; hydrophilicity; polycaprolactone; roughness; surface modification