title: Preliminary Evaluation of Platelet Rich Fibrin-Mediated Tissue Repair in Immature Canine Pulpless Teeth.
abstract: OBJECTIVE
  To evaluate the use of platelet-rich fibrin (PRF) in the regenerative therapy of immature canine permanent teeth.
  
  
  METHODS
  Eight immature premolars of beagle dogs were pulp extracted and cleaned with irrigation, then divided into two groups of empty root canals and those filled with a PRF clot. All of the eight premolars were sealed with mineral trioxide aggregate and glass ionomer cement. Two premolars were left naturally grown as a positive control. The root development was assessed radiographically and histologically after 12 weeks.
  
  
  RESULTS
  The radiological findings showed greater increases in the thickness of lateral dentinal wall in the PRF group than in the vacant group. Histologically, dental-associated mineral tissue, connective tissue, and bone-like mineral tissue grew into the root canals independent of PRF clot use. The PRF was able to increase the thickness of dental-associated mineral tissue. However, the vital tissue differed from the pulp dentin complex.
  
  
  CONCLUSIONS
  Our study demonstrated the feasibility of using PRF-mediated regenerative therapy in pulpless immature teeth for improving tissue repair.
authors.forename: Qi Lin
authors.initials: QL
authors.lastname: Wang
authors.forename: Pan Pan
authors.initials: PP
authors.lastname: Yang
authors.forename: Li Hong
authors.initials: LH
authors.lastname: Ge
authors.forename: He
authors.initials: H
authors.lastname: Liu
mesh_terms: D017607:Aluminum Compounds / Q000627:therapeutic use; D000818:Animals; D056624:Apexification / Q000379:methods*; D001641:Bicuspid / Q000187:drug effects / Q000473:pathology; D001792:Blood Platelets / Q000502:physiology*; D002113:Calcification, Physiologic / Q000187:drug effects; D017610:Calcium Compounds / Q000627:therapeutic use; D003238:Connective Tissue / Q000187:drug effects / Q000473:pathology; D003786:Dental Pulp Cavity / Q000187:drug effects* / Q000254:growth & development / Q000473:pathology; D003804:Dentin / Q000187:drug effects; D003809:Dentin, Secondary / Q000187:drug effects / Q000473:pathology; D004285:Dogs; D004338:Drug Combinations; D005337:Fibrin / Q000627:therapeutic use*; D005899:Glass Ionomer Cements / Q000737:chemistry; D008297:Male; D010087:Oxides / Q000627:therapeutic use; D012038:Regeneration / Q000187:drug effects; D012387:Root Canal Filling Materials / Q000627:therapeutic use; D012388:Root Canal Irrigants / Q000627:therapeutic use; D018915:Root Canal Preparation / Q000379:methods; D017640:Silicates / Q000627:therapeutic use; D007507:Therapeutic Irrigation / Q000379:methods; D019227:Tooth Apex / Q000187:drug effects* / Q000254:growth & development / Q000473:pathology
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D005899:Glass Ionomer Cements; D010087:Oxides; D012387:Root Canal Filling Materials; D012388:Root Canal Irrigants; D017640:Silicates; C086631:mineral trioxide aggregate; D005337:Fibrin