title: ADAM29 Expression in Human Breast Cancer and its Effects on Breast Cancer Cells In Vitro.
abstract: BACKGROUND
  A Disintegrin and Metalloprotease Domain 29 (ADAM29) is involved in many important physiological processes. Recent studies have demonstrated that ADAM29 is a susceptibility locus showing traits as a risk factor for breast cancer under genome-wide significance, however, the clinical relevance and cellular function of ADAM29 in breast cancer have not been reported.
  
  
  METHODS
  In this study, we assessed the expression levels of ADAM29 in a cohort of human breast cancer specimens. We also used MDA-MB-231 cells with differing ADAM29 expression and assessed the influence of ADAM29 and its mutations on the MDA-MB-231 cell line.
  
  
  RESULTS
  Increased transcript expression of ADAM29 was observed in breast cancer tissues compared to normal ones. The expression of ADAM29 and its mutations in different domains significantly influenced proliferation, migration and invasion of breast cancer cells in vitro.
  
  
  CONCLUSIONS
  ADAM29 may represent a prognostic factor in human breast cancer, as well as a novel molecular candidate to be used as a therapeutic target.
authors.affiliation: Department of Breast Surgery, Beijing Tian Tan Hospital, Capital Medical University, Beijing, P.R. China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, Beijing Tian Tan Hospital, Capital Medical University, Beijing, P.R. China.
authors.forename: Wang
authors.initials: W
authors.lastname: Jia
authors.affiliation: Department of Biochemistry and Molecular Biology, Capital Medical University, Beijing, P.R. China Beijing Key Laboratory of Cancer&Metastasis Research, Capital Medical University, Beijing, P.R. China Cardiff China Medical Research Collaborative, Cardiff University School of Medicine, Heath Park, Cardiff, U.K.
authors.forename: Wen G
authors.initials: WG
authors.lastname: Jiang
authors.affiliation: Department of Breast Surgery, Beijing Tian Tan Hospital, Capital Medical University, Beijing, P.R. China.
authors.forename: Pilin
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, Capital Medical University, Beijing, P.R. China Beijing Key Laboratory of Cancer&Metastasis Research, Capital Medical University, Beijing, P.R. China.
authors.forename: Guifang
authors.initials: G
authors.lastname: DU
authors.affiliation: Department of Biochemistry and Molecular Biology, Capital Medical University, Beijing, P.R. China Beijing Key Laboratory of Cancer&Metastasis Research, Capital Medical University, Beijing, P.R. China chengs@ccmu.edu.cn songmaomin@163.com.
authors.forename: Shan
authors.initials: S
authors.lastname: Cheng
authors.affiliation: Department of General Surgery, Beijing Tian Tan Hospital, Capital Medical University, Beijing, P.R. China chengs@ccmu.edu.cn songmaomin@163.com.
authors.forename: Maomin
authors.initials: M
authors.lastname: Song
mesh_terms: D051722:ADAM Proteins / Q000235:genetics / Q000378:metabolism*; D001943:Breast Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009154:Mutation; D009361:Neoplasm Invasiveness; D010641:Phenotype; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection; D015854:Up-Regulation
chemical_list: D051722:ADAM Proteins; C122189:ADAM29 protein, human
keywords: ADAM family; ADAM29; breast cancer mutation