title: Long Noncoding RNA Highly Upregulated in Liver Cancer Regulates the Tumor Necrosis Factor-α-Induced Apoptosis in Human Vascular Endothelial Cells.
abstract: Atherosclerosis is the major cause of myocardial infarction and stroke, which is a leading cause of morbidity and mortality in developed countries. During the pathological process of atherosclerosis, inflammation participates in all stages of atherosclerosis. Tumor necrosis factor-α (TNF-α), one of the most important inflammatory factor, induces apoptosis of endothelial cells, which play a central role in endothelial dysfunction. However, the underlying mechanism involved in long noncoding RNA (lncRNA) remains unclear. In the present study, we demonstrated the role of lncRNA highly upregulated in liver cancer (HULC) in TNF-α-induced apoptosis. HULC expression was decreased with TNF-α treatment. Restoring HULC expression rescued the apoptosis induced by TNF-α. HULC regulated TNF-α-induced apoptosis through regulation of miR-9 expression. Furthermore, RNA immunoprecipitation and RNA pull-down assays showed that HULC modulated miR-9 expression through association with DNA methyltransferases and suppression of miR-9 expression. HULC-miR-9 pathway may be a potential target for treating atherosclerosis.
authors.affiliation: Department of Cardiology, First Affiliated Hospital of General Hospital of Chinese People's Liberation Army , Beijing, China .
authors.forename: Yongjiang
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Cardiology, First Affiliated Hospital of General Hospital of Chinese People's Liberation Army , Beijing, China .
authors.forename: Dangsheng
authors.initials: D
authors.lastname: Huang
authors.affiliation: Department of Cardiology, First Affiliated Hospital of General Hospital of Chinese People's Liberation Army , Beijing, China .
authors.forename: Feifei
authors.initials: F
authors.lastname: Yang
authors.affiliation: Department of Cardiology, First Affiliated Hospital of General Hospital of Chinese People's Liberation Army , Beijing, China .
authors.forename: Miao
authors.initials: M
authors.lastname: Tian
authors.affiliation: Department of Cardiology, First Affiliated Hospital of General Hospital of Chinese People's Liberation Army , Beijing, China .
authors.forename: Yumei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Cardiology, First Affiliated Hospital of General Hospital of Chinese People's Liberation Army , Beijing, China .
authors.forename: Dong
authors.initials: D
authors.lastname: Shen
authors.affiliation: Department of Cardiology, First Affiliated Hospital of General Hospital of Chinese People's Liberation Army , Beijing, China .
authors.forename: Qiushuang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Cardiology, First Affiliated Hospital of General Hospital of Chinese People's Liberation Army , Beijing, China .
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Cardiology, First Affiliated Hospital of General Hospital of Chinese People's Liberation Army , Beijing, China .
authors.forename: Liwei
authors.initials: L
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis; D050197:Atherosclerosis / Q000276:immunology / Q000378:metabolism*; D018899:CpG Islands; D019175:DNA Methylation; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D011401:Promoter Regions, Genetic; D062085:RNA, Long Noncoding / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: C574685:HULC long non-coding RNA, human; C541874:MIRN92 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding; D014409:Tumor Necrosis Factor-alpha