title: Synthesis and emulsifying properties of carbohydrate fatty acid esters produced from Agave tequilana fructans by enzymatic acylation.
abstract: Carbohydrate fatty acid esters are non-ionic surfactants with a broad spectrum of applications. These molecules are generally synthesized using short carbohydrates or linear fructans; however in this research carbohydrate fatty acid esters were produced for the first time with branched fructans from Agave tequilana. Using immobilized lipases we successfully acylated A. tequilana fructans with vinyl laurate, obtaining products with different degrees of polymerization (DP). Lipozyme 435 was the most efficient lipase to catalyze the transesterification reaction. HPLC and ESI-MS analysis proved the presence of a mixture of acylated products as a result of the chemical complexity of fructans in the A. tequilana. The ESI-MS spectra showed a molecular mass shift between 183 and 366g/mol for fructooligosaccharides with a DP lower than 6, which indicated the presence of Agave fructans that had been mono- and diacylated with lauric acid. The carbohydrate fatty acid esters (CFAE) obtained showed good emulsifying properties in W/O emulsions.
authors.affiliation: Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Av. Normalistas 800, Col. Colinas de la Normal, C.P. 44270 Guadalajara, Jal., Mexico.
authors.forename: Leticia
authors.initials: L
authors.lastname: Casas-Godoy
authors.affiliation: Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Av. Normalistas 800, Col. Colinas de la Normal, C.P. 44270 Guadalajara, Jal., Mexico.
authors.forename: Javier
authors.initials: J
authors.lastname: Arrizon
authors.affiliation: Instituto Politécnico Nacional - CNMN, Calle Luis Enrique Erro s/n, Unidad Profesional Adolfo López Mateos, Col. Zacatenco, México D.F. CP 07738, Mexico.
authors.forename: Daniel
authors.initials: D
authors.lastname: Arrieta-Baez
authors.affiliation: Instituto de Catálisis y Petroleoquímica, CSIC, 28049 Madrid, Spain.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Plou
authors.affiliation: Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Av. Normalistas 800, Col. Colinas de la Normal, C.P. 44270 Guadalajara, Jal., Mexico. Electronic address: gsandoval@ciatej.mx.
authors.forename: Georgina
authors.initials: G
authors.lastname: Sandoval
mesh_terms: D000215:Acylation; D027882:Agave / Q000737:chemistry*; D055162:Biocatalysis; D004655:Emulsions / Q000737:chemistry*; D004952:Esters / Q000737:chemistry*; D005227:Fatty Acids / Q000737:chemistry*; D005630:Fructans / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry; D058105:Polymerization
chemical_list: D004655:Emulsions; D004952:Esters; D005227:Fatty Acids; D005630:Fructans; D009844:Oligosaccharides; D008049:Lipase
keywords: 1-Kestose (PubChem CID: 440080); Branched Agave tequilana fructans; Carbohydrate fatty acid esters; Emulsifiers; Enzymatic acylation; Lauric acid (PubChem CID: 3893); Lipases; Vinyl laurate (PubChem CID: 75069)