title: Infectious clones of the crinivirus cucurbit chlorotic yellows virus are competent for plant systemic infection and vector transmission.
abstract: Cucurbit chlorotic yellows virus (CCYV), a recently identified bipartite crinivirus, causes economic losses in cucurbit plants. CCYV is naturally transmitted only by whitefly Bemisia tabaci. Here we constructed full-length cDNA clones of CCYV (RNA1 and RNA2) fused to the T7 RNA polymerase promoter and the cauliflower mosaic virus 35S promoter. CCYV replicated and accumulated efficiently in Cucumis sativus protoplasts transfected with in vitro transcripts. Without RNA2, RNA1 replicated efficiently in C. sativus protoplasts. Agroinoculation with the infectious cDNA clones of CCYV resulted in systemic infection in the host plants of C. sativus and Nicotiana benthamiana. Virus derived from the infectious clones could be transmitted between cucumber plants by vector whiteflies. This system will greatly enhance the reverse genetic studies of CCYV gene functions.
authors.affiliation: College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Shi
authors.affiliation: College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, PR China.
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, PR China.
authors.forename: Qinsheng
authors.initials: Q
authors.lastname: Gu
authors.affiliation: College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, PR China.
authors.forename: Fengming
authors.initials: F
authors.lastname: Yan
authors.affiliation: College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, PR China.
authors.forename: Xinyan
authors.initials: X
authors.lastname: Sun
authors.affiliation: College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, PR China.
authors.forename: Honglian
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, PR China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Chen
authors.affiliation: College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, PR China.
authors.forename: Bingjian
authors.initials: B
authors.lastname: Sun
authors.affiliation: College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, PR China.
authors.forename: Zhenyue
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D029383:Crinivirus / Q000235:genetics* / Q000502:physiology*; D018553:Cucumis sativus / Q000821:virology*; D006430:Hemiptera / Q000821:virology*; D007303:Insect Vectors; D010935:Plant Diseases / Q000821:virology*; D014026:Nicotiana / Q000821:virology; D014779:Virus Replication