title: Efficient Absorption of X-Hydroxyproline (Hyp)-Gly after Oral Administration of a Novel Gelatin Hydrolysate Prepared Using Ginger Protease.
abstract: Recent studies have reported that oral intake of gelatin hydrolysate has various beneficial effects, such as reduction of joint pain and lowering of blood sugar levels. In this study, we produced a novel gelatin hydrolysate using a cysteine-type ginger protease having unique substrate specificity with preferential peptide cleavage with Pro at the P2 position. Substantial amounts of X-hydroxyproline (Hyp)-Gly-type tripeptides were generated up to 2.5% (w/w) concomitantly with Gly-Pro-Y-type tripeptides (5%; w/w) using ginger powder. The in vivo absorption of the ginger-degraded gelatin hydrolysate was estimated using mice. The plasma levels of collagen-derived oligopeptides, especially X-Hyp-Gly, were significantly high (e.g., 2.3-fold for Glu-Hyp-Gly, p < 0.05) compared with those of the control gelatin hydrolysate, which was prepared using gastrointestinal proteases and did not contain detectable X-Hyp-Gly. This study demonstrated that orally administered X-Hyp-Gly was effectively absorbed into the blood, probably due to the high protease resistance of this type of tripeptide.
authors.affiliation: Nippi Research Institute of Biomatrix , 520-11 Kuwabara, Toride, Ibaraki 302-0017, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Taga
authors.affiliation: Nippi Research Institute of Biomatrix , 520-11 Kuwabara, Toride, Ibaraki 302-0017, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Kusubata
authors.affiliation: Nippi Research Institute of Biomatrix , 520-11 Kuwabara, Toride, Ibaraki 302-0017, Japan.
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Ogawa-Goto
authors.affiliation: Nippi Research Institute of Biomatrix , 520-11 Kuwabara, Toride, Ibaraki 302-0017, Japan.
authors.forename: Shunji
authors.initials: S
authors.lastname: Hattori
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D055162:Biocatalysis; D002417:Cattle; D005780:Gelatin / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism; D020939:Zingiber officinale / Q000201:enzymology*; D006909:Hydroxyproline / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010447:Peptide Hydrolases / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry; D011492:Protein Hydrolysates / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D011492:Protein Hydrolysates; D005780:Gelatin; D010447:Peptide Hydrolases; D006909:Hydroxyproline
keywords: LC−MS; absorption; collagen; gelatin hydrolysate; ginger; hydroxyproline