title: Effects of selenium on ischaemia-reperfusion injury in a rat testis model.
abstract: Selenium is shown to have beneficial effects on ischaemia-reperfusion (IR) injury. Our aim was to assess the effects of selenium on IR-induced testicular damage in terms of biochemical and histopathological evaluation. A total of 32 rats were randomised into four groups: control, IR, IR + selenium (IR + S) and S. Detorsion was applied after 3 h of torsion. Testicular tissue superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), total antioxidant capacity (TAC) and DNA fragmentation levels were determined. Testicular tissue samples were examined by histopathological examination and terminal deoxynucleotidyl transferase dUTP nick end-labelling staining. The control, IR and IR + S groups had higher SOD values compared with the S group; SOD levels of the control and IR + S groups were higher than those of the IR group (P < 0.05). Further, MDA levels of the IR group were higher than those in the other three groups (P < 0.05). The IR group revealed lower TAC levels than the three groups (P < 0.05 for all). GSH levels of the IR group were significantly lower than those in the other three groups (P < 0.05 for all). In contrast, GSH levels of the IR + S group increased compared with those of the S group. The IR group had more DNA fragmentation than the control and S groups (P < 0.05). It is concluded that selenium possibly reduces oxidative stress and apoptosis caused by testicular IR injury in rats. The testicular protective effect of selenium appears to be mediated through its anti-apoptotic and antioxidative effects. However, selenium does not affect DNA fragmentation.
authors.affiliation: Department of Urology, Amasya University Faculty of Medicine, Amasya, Turkey.
authors.forename: Ö
authors.initials: Ö
authors.lastname: Kara
authors.affiliation: Department of Urology, Balikesir University Faculty of Medicine, Balikesir, Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Sari
authors.affiliation: Department of Biochemistry, Balikesir University Faculty of Veterinary, Balikesir, Turkey.
authors.forename: H
authors.initials: H
authors.lastname: Akşit
authors.affiliation: Department of Histology and Embriyology, Erciyes University Faculty of Medicine, Kayseri, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Yay
authors.affiliation: Department of Pharmacology, Balikesir University Faculty of Veterinary, Balikesir, Turkey.
authors.forename: D
authors.initials: D
authors.lastname: Akşit
authors.affiliation: Department of Urology, İstanbul University İstanbul Faculty of Medicine, İstanbul, Turkey.
authors.forename: M I
authors.initials: MI
authors.lastname: Dönmez
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004195:Disease Models, Animal; D005978:Glutathione / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D012643:Selenium / Q000494:pharmacology* / Q000627:therapeutic use; D013086:Spermatic Cord Torsion / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D013091:Spermatogenesis / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione; D012643:Selenium
keywords: Animal model; ischaemia; reperfusion injury; selenium; testicular torsion