title: Development of a low resolution (1)H NMR spectroscopic technique for the study of matrix mobility in fresh and freeze-thawed hen egg yolk.
abstract: Three experiments were conducted in developing a low resolution proton nuclear magnetic resonance ((1)H NMR) spectroscopic technique to study matrix mobility in fresh and freeze-thawed gelled yolk. The Carr-Purcell-Meiboom-Gill (CPMG) sequence was used to measure spin-spin relaxation times of proton pools representing major yolk constituents. A component identification test distinguished 3-4 pools. The least mobile pool was assigned to proteins, protein-lipid and protein-water interactions, and the most mobile to unbound water. The remaining pools were assigned to lipids, lipid-protein and lipid-water interactions. A stability test indicated that yolk had varied matrix mobility within the same sample across five days of refrigeration storage. A reproducibility test demonstrated high repeatability of fresh yolk measurements, but significant differences (p<0.05) were found within gelled yolk samples. This research determined that (1)H NMR spectroscopy, a non-destructive technique, can identify yolk components and detect changes in the matrix.
authors.affiliation: Department of Food Science and Human Nutrition, Iowa State University, Ames, IA 50011, United States.
authors.forename: Carmen
authors.initials: C
authors.lastname: Au
authors.affiliation: Department of Food Science and Human Nutrition, Iowa State University, Ames, IA 50011, United States. Electronic address: tongwang@iastate.edu.
authors.forename: Tong
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Food Science and Human Nutrition, Iowa State University, Ames, IA 50011, United States. Electronic address: nacevedo@iastate.edu.
authors.forename: Nuria C
authors.initials: NC
authors.lastname: Acevedo
mesh_terms: D000818:Animals; D002645:Chickens; D004530:Egg Yolk / Q000737:chemistry*; D004531:Eggs / Q000032:analysis / Q000592:standards; D005260:Female; D005615:Freezing; D005782:Gels; D008055:Lipids / Q000032:analysis*; D066244:Proton Magnetic Resonance Spectroscopy / Q000379:methods*; D012034:Refrigeration; D015203:Reproducibility of Results; D014867:Water / Q000032:analysis*
chemical_list: D005782:Gels; D008055:Lipids; D014867:Water
keywords: Egg yolk; Freeze-thawed gelled yolk; Low-resolution (1)H NMR; Matrix mobility