title: 2,4-D resistance in wild radish: reduced herbicide translocation via inhibition of cellular transport.
abstract: Resistance to auxinic herbicides is increasing in a range of dicotyledonous weed species, but in most cases the biochemical mechanism of resistance is unknown. Using (14)C-labelled herbicide, the mechanism of resistance to 2,4-dichlorophenoxyacetic acid (2,4-D) in two wild radish (Raphanus raphanistrum L.) populations was identified as an inability to translocate 2,4-D out of the treated leaf. Although 2,4-D was metabolized in wild radish, and in a different manner to the well-characterized crop species wheat and bean, there was no difference in metabolism between the susceptible and resistant populations. Reduced translocation of 2,4-D in the latter was also not due to sequestration of the herbicide, or to reduced uptake by the leaf epidermis or mesophyll cells. Application of auxin efflux or ABCB transporter inhibitors to 2,4-D-susceptible plants caused a mimicking of the reduced-translocation resistance phenotype, suggesting that 2,4-D resistance in the populations under investigation could be due to an alteration in the activity of a plasma membrane ABCB-type auxin transporter responsible for facilitating long-distance transport of 2,4-D.
authors.affiliation: Australian Herbicide Resistance Initiative, University of Western Australia, 35 Stirling Highway, Crawley 6009, Australia School of Plant Biology, University of Western Australia, 35 Stirling Highway, Crawley 6009, Australia danica.goggin@uwa.edu.au.
authors.forename: Danica E
authors.initials: DE
authors.lastname: Goggin
authors.affiliation: School of Plant Biology, University of Western Australia, 35 Stirling Highway, Crawley 6009, Australia.
authors.forename: Gregory R
authors.initials: GR
authors.lastname: Cawthray
authors.affiliation: Australian Herbicide Resistance Initiative, University of Western Australia, 35 Stirling Highway, Crawley 6009, Australia School of Plant Biology, University of Western Australia, 35 Stirling Highway, Crawley 6009, Australia.
authors.forename: Stephen B
authors.initials: SB
authors.lastname: Powles
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology*; D001693:Biological Transport, Active; D053209:Herbicide Resistance; D006540:Herbicides / Q000494:pharmacology*; D031224:Raphanus / Q000187:drug effects* / Q000378:metabolism
chemical_list: D006540:Herbicides; D015084:2,4-Dichlorophenoxyacetic Acid
keywords: ABCB transporters; Brassicaceae; auxin mimics; herbicide resistance; translocation; weeds.