title: Antifungal activity of a Saharan strain of Actinomadura sp. ACD1 against toxigenic fungi and other pathogenic microorganisms.
abstract: A new strain of actinobacteria, designated ACD1, was isolated from a Saharan soil sample in the Hoggar region (Algeria). Morphological study led to this strain being classified as a member of the Actinomadura genus. Phylogenetic analysis based on the 16S rRNA gene showed that the strain is closely related to Actinomadura sediminis DSM 45500(T) (98.5% sequence similarity). Furthermore, strain ACD1 presented a strong activity against mycotoxigenic and phytopathogenic fungi, including Aspergillus and Fusarium strains, and other pathogenic microorganisms. The kinetics of antimicrobial activity were investigated on ISP-2, Bennett and TSB media. Four solvents (n-hexane, dichloromethane, ethyl acetate and n-butanol) were used for the extraction of the produced antibiotic. The highest antimicrobial activity was obtained using the butanolic extract from the ISP-2 medium after seven days of fermentation culture. The active antibiotic was purified by reverse-phase HPLC using a C18 column. The UV-visible and mass spectra were determined. The minimum inhibitory concentrations (MIC) of this antibiotic were determined against pathogenic microorganisms.
authors.affiliation: Laboratoire de Biologie des Systèmes Microbiens (LBSM), École Normale Supérieure de Kouba, Alger, Algeria.
authors.forename: A
authors.initials: A
authors.lastname: Lahoum
authors.affiliation: Laboratoire de Biologie des Systèmes Microbiens (LBSM), École Normale Supérieure de Kouba, Alger, Algeria.
authors.forename: A
authors.initials: A
authors.lastname: Aouiche
authors.affiliation: Laboratoire de Biologie des Systèmes Microbiens (LBSM), École Normale Supérieure de Kouba, Alger, Algeria; Département de Biologie, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre, Université de Ghardaïa, BP 455, 47000 Ghardaïa, Algeria.
authors.forename: N
authors.initials: N
authors.lastname: Bouras
authors.affiliation: Laboratoire de Génie Chimique, LGC, Université de Toulouse, UMR 5503 (CNRS/INPT/UPS), Toulouse, France.
authors.forename: C
authors.initials: C
authors.lastname: Verheecke
authors.affiliation: School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne, NE1 7RU, United Kingdom.
authors.forename: H-P
authors.initials: HP
authors.lastname: Klenk
authors.affiliation: Laboratoire de Biologie des Systèmes Microbiens (LBSM), École Normale Supérieure de Kouba, Alger, Algeria. Electronic address: sabaou@yahoo.fr.
authors.forename: N
authors.initials: N
authors.lastname: Sabaou
authors.affiliation: Laboratoire de Génie Chimique, LGC, Université de Toulouse, UMR 5503 (CNRS/INPT/UPS), Toulouse, France.
authors.forename: F
authors.initials: F
authors.lastname: Mathieu
mesh_terms: D000192:Actinomycetales / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D000352:Africa, Northern; D000462:Algeria; D000935:Antifungal Agents / Q000378:metabolism* / Q000494:pharmacology*; D015373:Bacterial Typing Techniques; D004275:DNA, Ribosomal / Q000032:analysis; D005658:Fungi / Q000187:drug effects* / Q000254:growth & development / Q000472:pathogenicity; D008826:Microbial Sensitivity Tests; D012988:Soil Microbiology
chemical_list: D000935:Antifungal Agents; D004275:DNA, Ribosomal
keywords: Actinobacterium; Actinobactérie; Actinomadura; Activité antimicrobienne; Antimicrobial activity; Microorganismes pathogènes; Pathogenic microorganisms