title: [Determination of 8 hindered phenolic antioxidantsin olive oil by ultra-performance liquid chromatography and its migration research].
abstract: OBJECTIVE
  To develop a method for determination of PG, TBHQ, NDGA, BHA, Ionox100, OG, DG and BHT in olive oil by ultra-performance liquid chromatography (UPLC), and to study the migration rule of hindered phenolic antioxidants in food simulation solvent.
  
  
  METHODS
  The samples were extracted with acetonitrile, and C18 dispersion matrix solid-phase, the separation were performed by UPLC. The migration amount of PG, BHA, BHT from polyethylene film to different simulated solvent (olive oil, 20% ethanol, 10% ethanol, 3% acetic acid) were determined at 20, 60 and 100 degrees C.
  
  
  RESULTS
  The retention time of 8 hindered phenolic antioxidants was less than 8.5 min. The limits of detection were in the range of 0.01 - 0.05 μg/mL, and the linear correlations were > 0.999. The average recoveries were from 80.0% to 106.8%, and the relative standard deviations were between 0.69% and 7.94%. The original heating migration experiments of PG, BHA and BHT were performed by such method. As the extension of heating time, the migration amounts were increased, and the migration amounts of PG in water-based food simulation solvent was similar in olive oil. BHA and BHT easily migrated to simulate fat solvents. The migration amount was the maximum in olive oil, while the migration amounts of BHA and BHT were little as the ethanol decreased in other simulation solvents. The migration amount of BHA in 20% ethanol was more than in 10% ethanol and 3% acetic acid.
  
  
  CONCLUSIONS
  With the increase of temperature, the migration amount increase, and the hindered phenolic antioxidants easily migrate to simulate fat solvents.
authors.forename: Qifeng
authors.initials: Q
authors.lastname: Ma
authors.lastname: Zeng Fan'gang
authors.forename: Jie
authors.initials: J
authors.lastname: Lu
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol; D005520:Food Preservatives / Q000032:analysis* / Q000737:chemistry; D000069463:Olive Oil / Q000032:analysis* / Q000737:chemistry; D010636:Phenols / Q000032:analysis*; D012997:Solvents
chemical_list: D000975:Antioxidants; D005520:Food Preservatives; D000069463:Olive Oil; D010636:Phenols; D012997:Solvents; D000431:Ethanol