title: Physiological and proteomic responses to salt stress in chloroplasts of diploid and tetraploid black locust (Robinia pseudoacacia L.).
abstract: Salinity is an important abiotic stressor that negatively affects plant growth. In this study, we investigated the physiological and molecular mechanisms underlying moderate and high salt tolerance in diploid (2×) and tetraploid (4×) Robinia pseudoacacia L. Our results showed greater H2O2 accumulation and higher levels of important antioxidative enzymes and non-enzymatic antioxidants in 4× plants compared with 2× plants under salt stress. In addition, 4× leaves maintained a relatively intact structure compared to 2× leaves under a corresponding condition. NaCl treatment didn't significantly affect the photosynthetic rate, stomatal conductance or leaf intercellular CO2 concentrations in 4× leaves. Moreover, proteins from control and salt treated 2× and 4× leaf chloroplast samples were extracted and separated by two-dimensional gel electrophoresis. A total of 61 spots in 2× (24) and 4× (27) leaves exhibited reproducible and significant changes under salt stress. In addition, 10 proteins overlapped between 2× and 4× plants under salt stress. These identified proteins were grouped into the following 7 functional categories: photosynthetic Calvin-Benson Cycle (26), photosynthetic electron transfer (7), regulation/defense (5), chaperone (3), energy and metabolism (12), redox homeostasis (1) and unknown function (8). This study provides important information of use in the improvement of salt tolerance in plants.
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin 150040, P.R. China.
authors.forename: Fanjuan
authors.initials: F
authors.lastname: Meng
authors.affiliation: Key Laboratory of Saline-Alkaline Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Harbin, 150040, P.R. China.
authors.forename: Qiuxiang
authors.initials: Q
authors.lastname: Luo
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin 150040, P.R. China.
authors.forename: Qiuyu
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin 150040, P.R. China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin 150040, P.R. China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Qi
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin 150040, P.R. China.
authors.forename: Fuling
authors.initials: F
authors.lastname: Xu
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin 150040, P.R. China.
authors.forename: Xue
authors.initials: X
authors.lastname: Lei
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin 150040, P.R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Division of Plant Science, Research School of Biology, The Australian National University, 46 Biology Place, Acton 2601, ACT, Australia.
authors.forename: Wah Soon
authors.initials: WS
authors.lastname: Chow
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin 150040, P.R. China.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Sun
mesh_terms: D000975:Antioxidants / Q000502:physiology; D002736:Chloroplasts / Q000378:metabolism*; D004171:Diploidy; D006861:Hydrogen Peroxide / Q000378:metabolism; D010788:Photosynthesis; D018515:Plant Leaves / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D031301:Robinia / Q000235:genetics / Q000502:physiology*; D012965:Sodium Chloride / Q000378:metabolism* / Q000494:pharmacology; D013312:Stress, Physiological; D057891:Tetraploidy
chemical_list: D000975:Antioxidants; D010940:Plant Proteins; D020543:Proteome; D012965:Sodium Chloride; D006861:Hydrogen Peroxide