title: Micro-CT evaluation of the radioprotective effect of resveratrol on the mandibular incisors of irradiated rats.
abstract: The purpose of this study was to perform a microcomputed tomographic evaluation of the radioprotective effect of resveratrol on the volume of mandibular incisors of irradiated rats. A second aim was to make a quantitative assessment of the effect of x-ray exposure on these dental tissues. Twenty adult male rats were divided into four groups: control, irradiated control, resveratrol, and irradiated resveratrol. The resveratrol groups received 100 mg/kg of resveratrol, whereas the irradiated groups were exposed to 15 Gy of irradiation. The animals were sacrificed 30 days after the irradiation procedure, and their mandibles were removed and scanned in a microcomputed tomography unit. The images were loaded into Mimics software to allow segmentation of the mandibular incisor and assessment of its volume. The results were compared by One-way ANOVA and Tukey's post hoc test, considering a 5% significance level. The irradiated groups showed significantly diminished volumes of the evaluated teeth, as compared with the control group (p < 0.05). The resveratrol group presented higher values than those of the irradiated groups, and volumes similar to those of the control group. High radiation doses significantly affected tooth formation, resulting in alterations in the dental structure, and thus lower volumes. Moreover, resveratrol showed no effective radioprotective impact on dental tissues. Future studies are needed to evaluate different concentrations of this substance, in an endeavor to verify its potential as a radioprotector for these dental tissues.
authors.affiliation: Department of Oral Diagnosis, Piracicaba Dental School, Universidade de Campinas, Piracicaba, SP, Brazil.
authors.forename: Gabriella Lopes de Rezende
authors.initials: GL
authors.lastname: Barbosa
authors.affiliation: Department of Dental Ecology, School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
authors.forename: Luiz André
authors.initials: LA
authors.lastname: Pimenta
authors.affiliation: Department of Oral Diagnosis, Piracicaba Dental School, Universidade de Campinas, Piracicaba, SP, Brazil.
authors.forename: Solange Maria de
authors.initials: SM
authors.lastname: Almeida
mesh_terms: D000818:Animals; D021621:Imaging, Three-Dimensional; D007180:Incisor / Q000000981:diagnostic imaging / Q000187:drug effects / Q000528:radiation effects*; D008297:Male; D008334:Mandible / Q000000981:diagnostic imaging / Q000187:drug effects / Q000528:radiation effects*; D009805:Odontogenesis / Q000528:radiation effects; D011833:Radiation Injuries, Experimental / Q000000981:diagnostic imaging / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013997:Time Factors; D055114:X-Ray Microtomography / Q000379:methods*
chemical_list: D011837:Radiation-Protective Agents; D013267:Stilbenes; D000077185:Resveratrol