title: A single cytosine deletion in the OsPLS1 gene encoding vacuolar-type H+-ATPase subunit A1 leads to premature leaf senescence and seed dormancy in rice.
abstract: Leaf senescence is a programmed developmental process orchestrated by many factors, but its molecular regulation is not yet fully understood. In this study, a novel Oryza sativa premature leaf senescence mutant (ospls1) was examined. Despite normal development in early seedlings, the ospls1 mutant leaves displayed lesion-mimics and early senescence, and a high transpiration rate after tillering. The mutant also showed seed dormancy attributable to physical (defect of micropyle structure) and physiological (abscisic acid sensitivity) factors. Using a map-based cloning approach, we determined that a cytosine deletion in the OsPLS1 gene encoding vacuolar H(+)-ATPase subunit A1 (VHA-A1) underlies the phenotypic abnormalities in the ospls1 mutant. The OsPSL1/VHA-A1 transcript levels progressively declined with the age-dependent leaf senescence in both the ospls1 mutant and its wild type. The significant decrease in both OsPSL1/VHA-A1 gene expression and VHA enzyme activity in the ospls1 mutant strongly suggests a negative regulatory role for the normal OsPLS1/VHA-A1 gene in the onset of rice leaf senescence. The ospls1 mutant featured higher salicylic acid (SA) levels and reactive oxygen species (ROS) accumulation, and activation of signal transduction by up-regulation of WRKY genes in leaves. Consistent with this, the ospls1 mutant exhibited hypersensitivity to exogenous SA and/or H2O2 Collectively, these results indicated that the OsPSL1/VAH-A1 mutation played a causal role in premature leaf senescence through a combination of ROS and SA signals. To conclude, OsPLS1 is implicated in leaf senescence and seed dormancy in rice.
authors.affiliation: Department of Agronomy, Zijingang Campus, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Xi
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Agronomy, Zijingang Campus, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Pan
authors.initials: P
authors.lastname: Gong
authors.affiliation: Department of Agronomy, Zijingang Campus, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Kunyu
authors.initials: K
authors.lastname: Li
authors.affiliation: Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.
authors.forename: Fudeng
authors.initials: F
authors.lastname: Huang
authors.affiliation: Department of Agronomy, Zijingang Campus, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Fangmin
authors.initials: F
authors.lastname: Cheng
authors.affiliation: Department of Agronomy, Zijingang Campus, Zhejiang University, Hangzhou 310058, PR China pangang12@126.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Pan
mesh_terms: D000375:Aging / Q000235:genetics* / Q000502:physiology; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003596:Cytosine; D017343:Genes, Plant / Q000235:genetics* / Q000502:physiology; D012275:Oryza / Q000235:genetics* / Q000502:physiology; D057445:Plant Dormancy / Q000235:genetics / Q000502:physiology*; D018515:Plant Leaves / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D017421:Sequence Analysis; D017384:Sequence Deletion / Q000235:genetics / Q000502:physiology; D015398:Signal Transduction; D025262:Vacuolar Proton-Translocating ATPases / Q000235:genetics* / Q000502:physiology
chemical_list: D017382:Reactive Oxygen Species; D003596:Cytosine; D025262:Vacuolar Proton-Translocating ATPases
keywords: Oryza sativa L.; OsPLS1; ROS; SA; premature leaf senescence; seed dormancy; vacuolar-type H+-ATPase.