title: No change in mRNA expression of immune-related genes in peripheral blood mononuclear cells challenged with Theileria annulata in Murrah buffalo (Bubalus bubalis).
abstract: Water buffaloes (Bubalus bubalis) act as carrier to Theileria annulata and show less clinical sign of tropical theileriosis as compared to indigenous and exotic cattle. Differential expression of immune-related genes such as major histocompatibility complex, class II, DQ alpha 1 (MHC-DQα), signal-regulatory protein alpha (SIRPA), prion protein (PRNP), Toll-like receptor 10 (TLR10), c-musculoaponeurotic fibrosarcoma oncogene homolog (cMAF) and V-maf avian musculoaponeurotic fibrosarcoma oncogene homolog B (MAFB) genes influence host resistance to this disease in exotic, crossbred and indigenous cattle. In the present study we examined the differential mRNA expression of the abovesaid immune-related genes in response to T. annulata infection in buffaloes. Peripheral blood mononuclear cells (PBMCs) harvested from blood samples of buffaloes were challenged with ground-up tick supernatant carrying T. annulata sporozoites in vitro. After 48h of in vitro challenge qPCR was employed to measure the relative mRNA expression of MHC-DQα, SIRPA, PRNP, TLR10, cMAF and MAFB genes in infected and control PBMCs. In the current study, the selected genes showed no change in mRNA expression after T.annulata infection which indicates that they have little role in providing host resistance to theileriosis in buffaloes.
authors.affiliation: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India. Electronic address: drmanjit@ivri.res.in.
authors.forename: Manjit
authors.initials: M
authors.lastname: Panigrahi
authors.affiliation: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
authors.forename: Amod
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
authors.forename: Bharat
authors.initials: B
authors.lastname: Bhushan
authors.affiliation: Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
authors.forename: Srikant
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
authors.forename: B C
authors.initials: BC
authors.lastname: Saravanan
authors.affiliation: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
authors.forename: Sourabh
authors.initials: S
authors.lastname: Sulabh
authors.affiliation: Division of Pharmacology & Toxicology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
authors.forename: Subhashree
authors.initials: S
authors.lastname: Parida
authors.affiliation: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
authors.forename: Gyanendra Kumar
authors.initials: GK
authors.lastname: Gaur
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000276:immunology / Q000469:parasitology*; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D007109:Immunity / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000378:metabolism; D051256:Oncogene Protein v-maf / Q000235:genetics; D000072002:Prion Proteins / Q000235:genetics; D051257:Proto-Oncogene Proteins c-maf / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D011971:Receptors, Immunologic / Q000235:genetics; D016796:Theileria annulata / Q000737:chemistry / Q000276:immunology*; D013801:Theileriasis / Q000097:blood / Q000235:genetics* / Q000276:immunology* / Q000469:parasitology; D013987:Ticks / Q000469:parasitology; D051201:Toll-Like Receptor 10 / Q000235:genetics
chemical_list: D000949:Histocompatibility Antigens Class II; D051256:Oncogene Protein v-maf; D000072002:Prion Proteins; D051257:Proto-Oncogene Proteins c-maf; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D051201:Toll-Like Receptor 10
keywords: Buffalo; Hyalomma tick; Immune-related genes; Theileria annulata.