title: Ethanol production from lignocellulosic hydrolysates using engineered Saccharomyces cerevisiae harboring xylose isomerase-based pathway.
abstract: The efficient co-fermentation of glucose and xylose is necessary for the economically feasible bioethanol production from lignocellulosic biomass. Even with xylose utilizing Saccharomyces cerevisiae, the efficiency of the lignocellulosic ethanol production remains suboptimal mainly due to the low conversion yield of xylose to ethanol. In this study, we evaluated the co-fermentation performances of SXA-R2P-E, a recently engineered isomerase-based xylose utilizing strain, in mixed sugars and in lignocellulosic hydrolysates. In a high-sugar fermentation with 70g/L of glucose and 40g/L of xylose, SXA-R2P-E produced 50g/L of ethanol with an yield of 0.43gethanol/gsugars at 72h. From dilute acid-pretreated hydrolysates of rice straw and hardwood (oak), the strain produced 18-21g/L of ethanol with among the highest yield of 0.43-0.46gethanol/gsugars ever reported. This study shows a highly promising potential of a xylose isomerase-expressing strain as an industrially relevant ethanol producer from lignocellulosic hydrolysates.
authors.affiliation: Clean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea.
authors.forename: Ja Kyong
authors.initials: JK
authors.lastname: Ko
authors.affiliation: Clean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea.
authors.forename: Youngsoon
authors.initials: Y
authors.lastname: Um
authors.affiliation: Clean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea.
authors.forename: Han Min
authors.initials: HM
authors.lastname: Woo
authors.affiliation: Department of Biotechnology, Graduate School, Korea University, Seoul 02841, Republic of Korea.
authors.forename: Kyoung Heon
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Clean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea. Electronic address: smlee@kist.re.kr.
authors.forename: Sun-Mi
authors.initials: SM
authors.lastname: Lee
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000378:metabolism*; D018533:Biomass; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods*; D012275:Oryza / Q000737:chemistry; D029963:Quercus / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D014866:Waste Products / Q000032:analysis; D014934:Wood / Q000737:chemistry; D014994:Xylose / Q000378:metabolism
chemical_list: D014866:Waste Products; C036909:lignocellulose; D000431:Ethanol; D008031:Lignin; D014994:Xylose; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase; D005947:Glucose
keywords: Co-fermentation; Ethanol; Lignocellulosic hydrolysate; Saccharomyces cerevisiae; Xylose isomerase