title: Hypocrellin B and paclitaxel-encapsulated hyaluronic acid-ceramide nanoparticles for targeted photodynamic therapy in lung cancer.
abstract: To increase the therapeutic efficacy of photodynamic therapy (PDT) in treating lung cancer, we developed both photosensitizer and anticancer drug encapsulated hyaluronic acid-ceramide nanoparticles. Based on our previous study, a co-delivery system of photosensitizers and anticancer agents greatly improves the therapeutic effect of PDT. Furthermore, hyaluronic acid-ceramide-based nanoparticles are ideal targeting carriers for lung cancer. In vitro phototoxicity in A549 (human lung adenocarcinoma) cells and in vivo antitumor efficacy in A549 tumor-bearing mice treated with hypocrellin B (HB)-loaded nanoparticles (HB-NPs) or hypocrellin B and paclitaxel loaded nanoparticles (HB-P-NPs) were evaluated. Cell viability assay, microscopic analysis and FACS analysis were performed for the in vitro studies and HB-P-NPs showed enhanced phototoxicity compared with HB-NPs. In the animal study, the tumor volume change and the histological analysis was studied and the anticancer efficacy improved in the order of free HB<HB-NPs<HB-P-NPs. In conclusion, the combination therapy of PDT and chemotherapy, and hyaluronic acid-ceramide nanoparticle-based targeted delivery improved the effects of PDT in lung cancer in mice.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Seoul National University Bundang Hospital, Seongnam-Si, Gyeonggi-do, Republic of Korea.
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Chang
authors.affiliation: College of Pharmacy, Kangwon National University, Chuncheon, Republic of Korea.
authors.forename: Hyun-Jong
authors.initials: HJ
authors.lastname: Cho
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Seoul National University Bundang Hospital, Seongnam-Si, Gyeonggi-do, Republic of Korea.
authors.forename: Eunjue
authors.initials: E
authors.lastname: Yi
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: Dae-Duk
authors.initials: DD
authors.lastname: Kim
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Seoul National University Bundang Hospital, Seongnam-Si, Gyeonggi-do, Republic of Korea; Department of Thoracic and Cardiovascular Surgery, Seoul National University College of Medicine, Seoul, Republic of Korea. Electronic address: jheon@snu.ac.kr.
authors.forename: Sanghoon
authors.initials: S
authors.lastname: Jheon
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage*; D002518:Ceramides / Q000737:chemistry*; D064593:Heterografts; D006820:Hyaluronic Acid / Q000737:chemistry*; D008175:Lung Neoplasms / Q000188:drug therapy*; D051379:Mice; D017239:Paclitaxel / Q000008:administration & dosage*; D010569:Perylene / Q000008:administration & dosage / Q000031:analogs & derivatives*; D010778:Photochemotherapy; D011809:Quinones / Q000008:administration & dosage*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002518:Ceramides; D011809:Quinones; D010569:Perylene; D006820:Hyaluronic Acid; D017239:Paclitaxel; C064522:hypocrellin B
keywords: Hyaluronic acid–ceramide nanoparticle; Hypocrellin B; Lung cancer; Paclitaxel; Photodynamic therapy