title: Stereoselective analysis of novel chiral fungicide pyrisoxazole in cucumber, tomato and soil under different application methods with supercritical fluid chromatography/tandem mass spectrometry.
abstract: Various new chiral pesticides have been registered and used in crop yields. However, few studies have focused on the environmental behavior of such new registered chiral compounds on the stereoisomer level. In this study, an effective and sensitive chiral analytical method was first developed to detect pyrisoxazole stereoisomers and then further applied to investigate the stereoselective dissipation in vegetables and soil using supercritical fluid chromatography/tandem triple quadrupole mass spectrometry. Optimal separation condition was achieved with IA column using CO2/MeOH (75:25) as mobile phase at 2.0 mL/min in 5 min, 35 °C and 2400 psi. The average recoveries in all of the matrices at four spiking levels ranged from 84.0% to 105.6%. Significant stereoselective dissipation was observed in cucumber and tomato under both application modes. (-) Pyrisoxazole A and (-) pyrisoxazole B were preferentially degraded in cucumber under foliar spraying mode. In contrast, (+) pyrisoxazole A and (-) pyrisoxazole B were preferentially degraded in cucumber under soil irrigation mode. (-) Pyrisoxazole A and (-) pyrisoxazole B were degraded faster than their antipodes in tomato under both application modes. However, no significant stereoselectivity was observed in soil. The results of this study could help facilitate more accurate risk assessments of pyrisoxazole.
authors.affiliation: State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, PR China.
authors.forename: Xinglu
authors.initials: X
authors.lastname: Pan
authors.affiliation: State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, PR China.
authors.forename: Fengshou
authors.initials: F
authors.lastname: Dong
authors.affiliation: State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.affiliation: State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, PR China.
authors.forename: Xingang
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, PR China.
authors.forename: Zenglong
authors.initials: Z
authors.lastname: Chen
authors.affiliation: State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, PR China. Electronic address: zhengyongquan@ippcaas.cn.
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D025924:Chromatography, Supercritical Fluid; D018553:Cucumis sativus / Q000737:chemistry* / Q000378:metabolism; D005638:Fruit / Q000737:chemistry / Q000378:metabolism; D005659:Fungicides, Industrial / Q000032:analysis* / Q000378:metabolism; D018551:Solanum lycopersicum / Q000737:chemistry* / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D012989:Soil Pollutants / Q000032:analysis* / Q000378:metabolism; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry; D014230:Triazoles / Q000032:analysis* / Q000378:metabolism
chemical_list: D005659:Fungicides, Industrial; D012989:Soil Pollutants; D014230:Triazoles
keywords: Dissipation; Pyrisoxazole; SFC-MS/MS; Stereoselective