title: Simultaneous determination of sucralose and related compounds by high-performance liquid chromatography with evaporative light scattering detection.
abstract: Sucralose is widely used in food and beverages as sweetener. Current synthesis approaches typically provide sucralose products with varying levels of related chlorinated carbohydrates which can affect the taste and flavor-modifying properties of sucralose. Quantification of related compounds in sucralose is often hampered by the lack of commercially available standards. In this work, nine related compounds were purified (purity>97%) and identified by liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance (NMR), then a rapid and simple HPLC coupled with evaporative light scattering detection (ELSD) method has been developed for the simultaneous determination of sucralose and related compounds. Under optimized conditions, the method showed good linearity in the range of 2-600μgmL(-1) with determination coefficients R(2)⩾0.9990. Moreover, low limits of detection in the range of 0.5-2.0μgmL(-1) and good repeatability (RSD<3%, n=6) were obtained. Recoveries were from 96.8% to 101.2%. Finally, the method has been successfully applied to sucralose quality control and purification process monitoring.
authors.affiliation: Department of Chemistry, Zhejiang University, Hangzhou 310028, China.
authors.forename: Wenwu
authors.initials: W
authors.lastname: Yan
authors.affiliation: Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310028, Zhejiang, China.
authors.forename: Nani
authors.initials: N
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Zhejiang University, Hangzhou 310028, China.
authors.forename: Peimin
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Zhejiang University, Hangzhou 310028, China.
authors.forename: Jiajie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Zhejiang Institute of Geology & Mineral Resources, Hangzhou 310007, China.
authors.forename: Shuchao
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Chemistry, Zhejiang University, Hangzhou 310028, China. Electronic address: zhuyan@zju.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D000067493:Dynamic Light Scattering; D005504:Food Analysis / Q000379:methods*; D057230:Limit of Detection; D009682:Magnetic Resonance Spectroscopy; D011786:Quality Control; D015203:Reproducibility of Results; D013395:Sucrose / Q000031:analogs & derivatives* / Q000032:analysis; D013549:Sweetening Agents / Q000032:analysis
chemical_list: D013549:Sweetening Agents; D013395:Sucrose; C026285:trichlorosucrose
keywords: HPLC–ELSD; Purification; Quality control; Related compounds; Sucralose