title: Photo-protective effect of sargachromenol against UVB radiation-induced damage through modulating cellular antioxidant systems and apoptosis in human keratinocytes.
abstract: The aim of this study was to evaluate the photo-preventive effects of sargachromenol (SC) against ultraviolet B (UVB)-induced oxidative stress in human keratinocytes via assessing the antioxidant properties and underlying molecular mechanisms. SC exhibited a significant scavenging effect on UVB-induced intracellular reactive oxygen species (ROS). SC attenuated UVB-induced oxidative macromolecular damage, including the protein carbonyl content, DNA strand break, and 8-isoprostane level. Furthermore, SC decreased UVB-induced Bax, cleaved caspase-9, and cleaved caspase-3 protein levels, but increased that of Bcl-2, which are well-known key mediators of apoptosis. Moreover, SC increased superoxide dismutase, catalase, and heme oxygenase-1 protein expression. Pre-treatment with SC upregulated the main transcription factor of antioxidant enzymes, erythroid 2-related factor 2 level, which was reduced by UVB irradiation. Extracellular signal-regulated kinase (ERK) and Jun N-terminal kinases (JNK) are involved in the regulation of many cellular events, including apoptosis. SC treatment reversed ERK and JNK activation induced by UVB. Collectively, these data indicate that SC can provide remarkable cytoprotection against the adverse effects of UVB radiation by modulating cellular antioxidant systems, and suggest the potential of developing a medical agent for ROS-induced skin diseases.
authors.affiliation: Department of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Pattage Madushan Dilhara Jayatissa
authors.initials: PM
authors.lastname: Fernando
authors.affiliation: Department of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Mei Jing
authors.initials: MJ
authors.lastname: Piao
authors.affiliation: Department of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Susara Ruwan Kumara Madduma
authors.initials: SR
authors.lastname: Hewage
authors.affiliation: Department of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Hee Kyoung
authors.initials: HK
authors.lastname: Kang
authors.affiliation: Department of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Eun Sook
authors.initials: ES
authors.lastname: Yoo
authors.affiliation: Department of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Young Sang
authors.initials: YS
authors.lastname: Koh
authors.affiliation: Jeju Biodiversity Research Institute, Jeju Technopark, Jeju 63243, Republic of Korea.
authors.forename: Mi Hee
authors.initials: MH
authors.lastname: Ko
authors.affiliation: Jeju Biodiversity Research Institute, Jeju Technopark, Jeju 63243, Republic of Korea.
authors.forename: Chang Sik
authors.initials: CS
authors.lastname: Ko
authors.affiliation: Department of Chemistry and Cosmetics, College of Natural Sciences, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Byeon
authors.affiliation: Department of Chemistry and Cosmetics, College of Natural Sciences, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Seung Ri
authors.initials: SR
authors.lastname: Mun
authors.affiliation: Department of Chemistry and Cosmetics, College of Natural Sciences, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Nam Ho
authors.initials: NH
authors.lastname: Lee
authors.affiliation: Department of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, Republic of Korea. Electronic address: jinwonh@jejunu.ac.kr.
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Hyun
mesh_terms: D001578:Benzopyrans / Q000494:pharmacology*; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D019610:Cytoprotection; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000502:physiology / Q000528:radiation effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013473:Sunscreening Agents / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D001578:Benzopyrans; D017382:Reactive Oxygen Species; D013473:Sunscreening Agents; C503204:sargachromenol; D002374:Catalase; D013482:Superoxide Dismutase; D048049:Extracellular Signal-Regulated MAP Kinases; D053148:Caspase 3; D053453:Caspase 9
keywords: Human keratinocytes; Reactive oxygen species; Sargachromenol; Ultraviolet B radiation