title: Expression of Semaphorin 3C in Breast Cancer and its Impact on Adhesion and Invasion of Breast Cancer Cells.
abstract: BACKGROUND
  The aim of the current study was to examine the role of semaphorin 3C (SEMA3C) in breast cancer.
  
  
  METHODS
  SEMA3C transcripts expressed by breast tissues were determined using real-time polymerase chain reaction (PCR). Knock-down of SEMA3C was performed in MDA-MB-231 and MCF-7 breast cancer cell lines using anti-SEMA3C hammerhead ribozyme transgenes. The effect of SEMA3C knockdown on cancer cells was determined using in vitro cellular function assays.
  
  
  RESULTS
  Higher SEMA3C transcript levels were significantly associated with poor differentiation of cancer cells, and transcript levels were significantly reduced in oestrogen receptor-positive tumours. Knock-down of SEMA3C expression resulted in a decrease in cell proliferation, adhesion and invasion of breast cancer cells.
  
  
  CONCLUSIONS
  Higher SEMA3C expression is correlated with tumour differentiation. Inhibition of SEMA3C reduces adhesion and invasion of breast cancer cells. This suggests that SEMA3C may play a significant role in morphological changes of cancer cells, leading to enhanced growth and dissemination.
authors.affiliation: Metastasis and Angiogenesis Research Group, Cardiff University-Peking University Cancer Institute, Cardiff University School of Medicine, Cardiff, U.K. Department of Biosciences, COMSATS-Institute of Information Technology, Islamabad, Pakistan.
authors.forename: Muhammad Faraz Arshad
authors.initials: MF
authors.lastname: Malik
authors.affiliation: Metastasis and Angiogenesis Research Group, Cardiff University-Peking University Cancer Institute, Cardiff University School of Medicine, Cardiff, U.K.
authors.forename: Lucy K
authors.initials: LK
authors.lastname: Satherley
authors.affiliation: Cardiff Breast Centre, University Hospital Llandough, Cardiff and Vale University Health Board, Cardiff, U.K.
authors.forename: Eleri L
authors.initials: EL
authors.lastname: Davies
authors.affiliation: Metastasis and Angiogenesis Research Group, Cardiff University-Peking University Cancer Institute, Cardiff University School of Medicine, Cardiff, U.K.
authors.forename: Lin
authors.initials: L
authors.lastname: Ye
authors.affiliation: Metastasis and Angiogenesis Research Group, Cardiff University-Peking University Cancer Institute, Cardiff University School of Medicine, Cardiff, U.K. JiangW@Cardiff.ac.uk.
authors.forename: Wen G
authors.initials: WG
authors.lastname: Jiang
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002448:Cell Adhesion; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D061986:MCF-7 Cells; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D016337:RNA, Catalytic / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism; D039961:Semaphorins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014162:Transfection
chemical_list: D016337:RNA, Catalytic; D012333:RNA, Messenger; D011960:Receptors, Estrogen; C543908:Sema3C protein, human; D039961:Semaphorins; C427204:hammerhead ribozyme
keywords: Breast cancer; differentiation; semaphorin 3C