title: Improved sensitivity of lateral flow assay using paper-based sample concentration technique.
abstract: Lateral flow assays (LFAs) hold great promise for point-of-care testing, especially in resource-poor settings. However, the poor sensitivity of LFAs limits their widespread applications. To address this, we developed a novel device by integrating dialysis-based concentration method into LFAs. The device successfully achieved 10-fold signal enhancement in Human Immunodeficiency Virus (HIV) nucleic acid detection with a detection limit of 0.1 nM and 4-fold signal enhancement in myoglobin (MYO) detection with a detection limit of 1.56 ng/mL in less than 25 min. This simple, low-cost and portable integrated device holds great potential for highly sensitive detection of various target analytes for medical diagnostics, food safety analysis and environmental monitoring.
authors.affiliation: School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, PR China; Key Laboratory for Space Bioscience and Biotechnology, Northwestern Polytechnical University, Xi'an 710072, PR China; The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, PR China.
authors.forename: Ruihua
authors.initials: R
authors.lastname: Tang
authors.affiliation: School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, PR China; Key Laboratory for Space Bioscience and Biotechnology, Northwestern Polytechnical University, Xi'an 710072, PR China. Electronic address: kittyyh@nwpu.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.affiliation: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, PR China; Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia.
authors.forename: Jane Ru
authors.initials: JR
authors.lastname: Choi
authors.affiliation: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Gong
authors.affiliation: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Hu
authors.affiliation: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, PR China.
authors.forename: Shangsheng
authors.initials: S
authors.lastname: Feng
authors.affiliation: Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia.
authors.forename: Belinda
authors.initials: B
authors.lastname: Pingguan-Murphy
authors.affiliation: School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, PR China; Key Laboratory for Space Bioscience and Biotechnology, Northwestern Polytechnical University, Xi'an 710072, PR China.
authors.forename: Qibing
authors.initials: Q
authors.lastname: Mei
authors.affiliation: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, PR China. Electronic address: fengxu@mail.xjtu.edu.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
mesh_terms: D001681:Biological Assay; D003956:Dialysis; D006046:Gold / Q000737:chemistry; D006678:HIV / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D009211:Myoglobin / Q000032:analysis*; D019095:Point-of-Care Systems; D012367:RNA, Viral / Q000032:analysis*
chemical_list: D009211:Myoglobin; D012367:RNA, Viral; D006046:Gold
keywords: Dialysis-based concentration; HIV; Lateral flow assay; MYO; Paper-based device