title: Comparison of three methods for the detection of Angiostrongylus vasorum in the final host.
abstract: Angiostrongylosis is potentially fatal parasitic nematode infection affecting dogs which can be difficult to diagnose. In recent years several microscopical, serological and molecular detection methods have been developed, however there are few studies that have compared the relative performance of these methods. Screening necropsy material from an opportunistic sample of 140 foxes (82 of which were considered to be infected with Angiostrongylus vasorum), indicated sensitivities of 84.1% for dissection and visual examination of plucks, 69.5% for nested PCR of an 18S rRNA fragment and 76.8% for a canine A. vasorum antigen detection test (IDEXX Angio Detect) of tissue fluid samples respectively. Agreement between the tests ranged from 45.6 to 79.7%. A novel nested PCR-RFLP for the detection and identification of canid lungworm spp. is described.
authors.affiliation: Ecole Nationale Vétérinaire de Toulouse (ENVT), France. Electronic address: houpin.emilie@gmail.com.
authors.forename: E
authors.initials: E
authors.lastname: Houpin
authors.affiliation: School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: G
authors.initials: G
authors.lastname: McCarthy
authors.affiliation: Department de Genie Biologique, Institut Universitaire de Technologie, Universite Claude Bernard, Lyon, France. Electronic address: maxence.ferrand@etu.univ-lyon1.fr.
authors.forename: M
authors.initials: M
authors.lastname: Ferrand
authors.affiliation: School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: T
authors.initials: T
authors.lastname: De Waal
authors.affiliation: School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: E J
authors.initials: EJ
authors.lastname: O'Neill
authors.affiliation: School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland. Electronic address: annetta.zintl@ucd.ie.
authors.forename: A
authors.initials: A
authors.lastname: Zintl
mesh_terms: D000801:Angiostrongylus / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis; D004210:Dissection / Q000592:standards*; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards*; D005589:Foxes / Q000469:parasitology*; D025202:Molecular Diagnostic Techniques / Q000592:standards*; D010274:Parasitology / Q000379:methods*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017206:Strongylida Infections / Q000175:diagnosis*
chemical_list: D000947:Antigens, Helminth; D012337:RNA, Ribosomal, 18S
keywords: Angiostrongylus vasorum; Crenosoma vulpis; Diagnosis; IDEXX Angio Detect kit; Nested PCR-RFLP; Post-mortem examination; Vulpes vulpes