title: Schisandra polysaccharide increased glucose consumption by up-regulating the expression of GLUT-4.
abstract: In our previous study, a polysaccharide was extracted from Schisandra Chinensis (Trucz.) Baill and found with anti-diabetic effects. The aim of this study was to investigate the anti-diabetic effects of the low weight molecular polysaccharide (SCPP11) purified from crude Schisandra polysaccharide and illustrate the underlying mechanism in buffalo rat liver cells. The insulin resistance model of BRL cells was established by incubating with insulin solution for 24h. The effects of SCPP11 on regulating related protein and mRNA expression in an insulin and AMPK signal pathway were investigated by western blot and RT-PCR analysis. SCPP11 showed no cytotoxicity to BRL cells and could improve the glucose consumption in BRL cells. SCPP11 increased the protein expression of Akt, p-AMPK and GLUT-4 in BRL cells. Moreover, SCPP11 could enhance the mRNA expression levels of IRS-1, PI3K, Akt, GLUT-4, AMPKα and PPAR-γ in BRL cells at the same time. In conclusion, SCPP11 possessed effects in improving glucose consumption by up-regulating the expression of GLUT-4 which might occur via insulin and AMPK signal pathway and could be a potential functional food to prevent and mitigate the insulin resistance condition.
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Rd., Zhenjiang 212013, Jiangsu, China.
authors.forename: Dun
authors.initials: D
authors.lastname: Jin
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Rd., Zhenjiang 212013, Jiangsu, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhao
authors.affiliation: School of the Environment and Safety Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Feng
authors.affiliation: School of the Environment and Safety Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Guang-Hua
authors.initials: GH
authors.lastname: Mao
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Rd., Zhenjiang 212013, Jiangsu, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zou
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Rd., Zhenjiang 212013, Jiangsu, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Rd., Zhenjiang 212013, Jiangsu, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: School of the Environment and Safety Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Rd., Zhenjiang 212013, Jiangsu, China.
authors.forename: Xin-Tong
authors.initials: XT
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Rd., Zhenjiang 212013, Jiangsu, China. Electronic address: yangliuqing@ujs.edu.cn.
authors.forename: Liu-Qing
authors.initials: LQ
authors.lastname: Yang
authors.affiliation: School of the Environment and Safety Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China. Electronic address: wuxy@ujs.edu.cn.
authors.forename: Xiang-Yang
authors.initials: XY
authors.lastname: Wu
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D005947:Glucose / Q000378:metabolism*; D051275:Glucose Transporter Type 4 / Q000235:genetics* / Q000378:metabolism*; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism; D007333:Insulin Resistance; D047495:PPAR gamma / Q000235:genetics; D010750:Phosphoproteins / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D032141:Schisandra / Q000737:chemistry*; D015398:Signal Transduction / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D051275:Glucose Transporter Type 4; D007004:Hypoglycemic Agents; D007328:Insulin; D047495:PPAR gamma; D010750:Phosphoproteins; D011134:Polysaccharides; D012333:RNA, Messenger; D051057:Proto-Oncogene Proteins c-akt; D055372:AMP-Activated Protein Kinases; C568305:Prkaa1 protein, rat; D005947:Glucose
keywords: Diabetes; GLUT-4; S. chinensis (Trucz.) baill polysaccharide