title: GABARAP activates ULK1 and traffics from the centrosome dependent on Golgi partners WAC and GOLGA2/GM130.
abstract: WAC and GOLGA2/GM130 are 2 Golgi proteins that affect autophagy; however, their mechanism of action was unknown. We have shown that WAC binding to GOLGA2 at the Golgi displaces GABARAP from GOLGA2 to allow the maintenance of a nonlipidated centrosomal GABARAP pool. Centrosomal GABARAP can traffic to autophagic structures during starvation. In addition GABARAP specifically promotes ULK1 activation and this is independent of GABARAP lipidation but likely requires a LIR-mediated GABARAP-ULK1 interaction.
authors.affiliation: a The Francis Crick Institute, Lincoln's Inn Fields Laboratories , London , UK.
authors.forename: Justin
authors.initials: J
authors.lastname: Joachim
authors.affiliation: a The Francis Crick Institute, Lincoln's Inn Fields Laboratories , London , UK.
authors.forename: Sharon A
authors.initials: SA
authors.lastname: Tooze
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D051017:Apoptosis Regulatory Proteins; D001324:Autoantigens / Q000378:metabolism*; D001343:Autophagy / Q000502:physiology*; D000071189:Autophagy-Related Protein-1 Homolog / Q000378:metabolism*; D018385:Centrosome / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; D001324:Autoantigens; C116741:GABARAP protein, human; C097741:Golgin subfamily A member 2; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D000071189:Autophagy-Related Protein-1 Homolog
keywords: Atg8; GABARAP; GM130; GOLGA2; ULK1; WAC; centrosome; golgi; photoactivation