title: Application of small angle X-ray scattering synchrotron technology for measuring ovine meat quality.
abstract: A small angle X-ray scattering (SAXS) synchrotron was used to evaluate 100 ovine m. longissimus lumborum, representing lamb (n=50) and sheep (n=50). The diffraction of X-rays gives information on muscle myofibril structure and fat content. The linear relationships between SAXS measures with measures such as, shear force, intramuscular fat content (IMF) and collagen content/solubility, were investigated. A relationship was found between the d-spacing of the actin/myosin fibril spacing (SAX1 and SAX2) and the cross sectional area of the rhombohedral unit cell (Cell area) and shear force after 1 and 5day ageing. There was a positive relationship between IMF and a SAXS Fat area measure. There was a muscle site effect on SAX1, SAX2 and Cell area, with the cranial site having a larger distance between myofibrils. The potential of SAXS as a powerful research tool to determine not only the structural components of ovine tenderness, but also the fat content related to IMF is evident.
authors.affiliation: NSW Department of Primary Industries, Centre for Red Meat and Sheep Development, PO Box 129, Cowra, NSW 2794, Australia. Electronic address: jordan.hoban@dpi.nsw.gov.au.
authors.forename: J M
authors.initials: JM
authors.lastname: Hoban
authors.affiliation: NSW Department of Primary Industries, Centre for Red Meat and Sheep Development, PO Box 129, Cowra, NSW 2794, Australia.
authors.forename: D L
authors.initials: DL
authors.lastname: Hopkins
authors.affiliation: Australian Synchrotron, 800 Blackburn Road, Clayton, VIC 3168, Australia.
authors.forename: N
authors.initials: N
authors.lastname: Kirby
authors.affiliation: NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Private Bag 4008, Narellan, NSW 2567, Australia.
authors.forename: D
authors.initials: D
authors.lastname: Collins
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, Australia.
authors.forename: F R
authors.initials: FR
authors.lastname: Dunshea
authors.affiliation: Agriculture Research, Department of Economic Development, Jobs, Transport & Resources, 475 Mickleham Road, Attwood, VIC 3049, Australia.
authors.forename: M G
authors.initials: MG
authors.lastname: Kerr
authors.affiliation: NSW Department of Primary Industries, Wagga Wagga Agricultural Institute, Pine Gully Rd, PMB, Wagga Wagga, NSW 2650, Australia.
authors.forename: K
authors.initials: K
authors.lastname: Bailes
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, Australia.
authors.forename: J J
authors.initials: JJ
authors.lastname: Cottrell
authors.affiliation: NSW Department of Primary Industries, Centre for Red Meat and Sheep Development, PO Box 129, Cowra, NSW 2794, Australia.
authors.forename: B W B
authors.initials: BW
authors.lastname: Holman
authors.affiliation: Agriculture Research, Department of Economic Development, Jobs, Transport & Resources, 475 Mickleham Road, Attwood, VIC 3049, Australia.
authors.forename: W
authors.initials: W
authors.lastname: Brown
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, Australia; Agriculture Research, Department of Economic Development, Jobs, Transport & Resources, 475 Mickleham Road, Attwood, VIC 3049, Australia.
authors.forename: E N
authors.initials: EN
authors.lastname: Ponnampalam
mesh_terms: D000273:Adipose Tissue; D000818:Animals; D005504:Food Analysis / Q000295:instrumentation* / Q000379:methods; D063427:Food Quality; D008460:Meat / Q000592:standards*; D012756:Sheep; D017356:Synchrotrons / Q000295:instrumentation*; D014961:X-Ray Diffraction / Q000295:instrumentation* / Q000379:methods
keywords: Intramuscular fat content; Lamb; Meat quality; Particle size analysis; Sarcomere length; Shear force; X-ray diffraction