title: MicroRNA expression profile of human periodontal ligament cells under the influence of Porphyromonas gingivalis LPS.
abstract: Periodontitis is a chronic inflammatory disease which is caused by bacterial infection and leads to the destruction of periodontal tissues and resorption of alveolar bone. Thus, special attention should be paid to the mechanism under lipopolysaccharide (LPS)-induced periodontitis because LPS is the major cause of periodontitis. However, to date, miRNA expression in the LPS-induced periodontitis has not been well characterized. In this study, we investigated miRNA expression patterns in LPS-treated periodontal ligament cells (PDLCs). Through miRNA array and differential analysis, 22 up-regulated miRNAs and 28 down-regulated miRNAs in LPS-treated PDLCs were identified. Seven randomly selected up-regulated (miR-21-5p, 498, 548a-5p) and down-regulated (miR-495-3p, 539-5p, 34c-3p and 7a-2-3p) miRNAs were examined by qRT-PCR, and the results proved the accuracy of the miRNA array. Moreover, targets of these deregulated miRNAs were analysed using the miRWalk database. Database for Annotation, Visualization and Integration Discovery software were performed to analyse the Gene Ontology and Kyoto Encyclopaedia of Genes and Genomes pathway of differential expression miRNAs, and the results shown that Toll-like receptor signalling pathway, cAMP signalling pathway, transforming growth factor-beta signalling pathway, mitogen-activated protein kinase (MAPK) signalling pathway and other pathways were involved in the molecular mechanisms underlying LPS-induced periodontitis. In conclusion, this study provides clues for enhancing our understanding of the mechanisms and roles of miRNAs as key regulators of LPS-induced periodontitis.
authors.affiliation: Department of Stomatology, JinShan Hospital, FuDan University, JinShan District, ShangHai, China.
authors.forename: Anqing
authors.initials: A
authors.lastname: Du
authors.affiliation: Department of Orthodontics, Dental Hospital of HeNan Province, ZhengZhou University, ZhengZhou, HeNan, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Orthodontics, State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, ChengDu, China.
authors.forename: LingYun
authors.initials: L
authors.lastname: Wan
authors.affiliation: Key Laboratory of Oral Medicine, GuangZhou Institute of Oral Disease, Stomatology Hospital of GuangZhou Medical University, GuangZhou, China.
authors.forename: TianTao
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Stomatology, The First Teaching Hospital of Xinjiang Medical University, Urumqi, China.
authors.forename: Zaoxia
authors.initials: Z
authors.lastname: Peng
authors.affiliation: Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, China.
authors.forename: ZiYu
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Affliated Stomatological Hospital of NanChang University, NanChang, JiangXi Province, China.
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Liao
authors.affiliation: Department of Pharmacy, JinShan Hospital, FuDan University, JinShan District, ShangHai, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Fang
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D016000:Cluster Analysis; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D020869:Gene Expression Profiling; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D010513:Periodontal Ligament / Q000166:cytology*; D016966:Porphyromonas gingivalis / Q000737:chemistry*; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D015398:Signal Transduction / Q000235:genetics; D013997:Time Factors; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
chemical_list: D008070:Lipopolysaccharides; D035683:MicroRNAs
keywords: LPS; P. gingivalis; PDLCs; miRNAs; periodontitis