title: Arabidopsis CLAVATA1 and CLAVATA2 receptors contribute to Ralstonia solanacearum pathogenicity through a miR169-dependent pathway.
abstract: Bacterial wilt caused by Ralstonia solanacearum is one of the most destructive bacterial plant diseases. Although many molecular determinants involved in R. solanacearum adaptation to hosts and pathogenesis have been described, host components required for disease establishment remain poorly characterized. Phenotypical analysis of Arabidopsis mutants for leucine-rich repeat (LRR)-receptor-like proteins revealed that mutations in the CLAVATA1 (CLV1) and CLAVATA2 (CLV2) genes confer enhanced disease resistance to bacterial wilt. We further investigated the underlying mechanisms using genetic, transcriptomic and molecular approaches. The enhanced resistance of both clv1 and clv2 mutants to the bacteria did not require the well characterized CLV signalling modules involved in shoot meristem homeostasis, and was conditioned by neither salicylic acid nor ethylene defence-related hormones. Gene expression microarray analysis performed on clv1 and clv2 revealed deregulation of genes encoding nuclear transcription factor Y subunit alpha (NF-YA) transcription factors whose post-transcriptional regulation is known to involve microRNAs from the miR169 family. Both clv mutants showed a defect in miR169 accumulation. Conversely, overexpression of miR169 abrogated the resistance phenotype of clv mutants. We propose that CLV1 and CLV2, two receptors involved in CLV3 perception during plant development, contribute to bacterial wilt through a signalling pathway involving the miR169/NF-YA module.
authors.affiliation: Laboratoire des Interactions Plantes-Microorganismes (LIPM), INRA, UMR441, Chemin de Borde Rouge, F-31326, Castanet-Tolosan, France.|Laboratoire des Interactions Plantes-Microorganismes (LIPM), CNRS, UMR2594, Chemin de Borde Rouge, F-31326, Castanet-Tolosan, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Hanemian
authors.affiliation: Laboratoire des Interactions Plantes-Microorganismes (LIPM), INRA, UMR441, Chemin de Borde Rouge, F-31326, Castanet-Tolosan, France.|Laboratoire des Interactions Plantes-Microorganismes (LIPM), CNRS, UMR2594, Chemin de Borde Rouge, F-31326, Castanet-Tolosan, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Barlet
authors.affiliation: CNRS, Institut des Sciences du Végétal, Saclay Plant Sciences, UPR2355, 91198, Gif-sur-Yvette, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Sorin
authors.affiliation: Laboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, the Netherlands.
authors.forename: Koste A
authors.initials: KA
authors.lastname: Yadeta
authors.affiliation: INRA, Univ. Nice Sophia Antipolis, CNRS, UMR 1355-7254 Institut Sophia Agrobiotech, 06900, Sophia Antipolis, France.
authors.forename: Harald
authors.initials: H
authors.lastname: Keller
authors.affiliation: INRA, Univ. Nice Sophia Antipolis, CNRS, UMR 1355-7254 Institut Sophia Agrobiotech, 06900, Sophia Antipolis, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Favery
authors.affiliation: Institut für Entwicklungsgenetik, Heinrich-Heine-Universität, Universitätstr. 1, 40225, Düsseldorf, Germany.
authors.forename: Rüdiger
authors.initials: R
authors.lastname: Simon
authors.affiliation: Laboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, the Netherlands.
authors.forename: Bart P H J
authors.initials: BP
authors.lastname: Thomma
authors.affiliation: Université Paris Diderot, 5 rue Thomas Mann, 75205, Paris Cedex 13, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Hartmann
authors.affiliation: CNRS, Institut des Sciences du Végétal, Saclay Plant Sciences, UPR2355, 91198, Gif-sur-Yvette, France.
authors.forename: Martin
authors.initials: M
authors.lastname: Crespi
authors.affiliation: Laboratoire des Interactions Plantes-Microorganismes (LIPM), INRA, UMR441, Chemin de Borde Rouge, F-31326, Castanet-Tolosan, France.|Laboratoire des Interactions Plantes-Microorganismes (LIPM), CNRS, UMR2594, Chemin de Borde Rouge, F-31326, Castanet-Tolosan, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Marco
authors.affiliation: Laboratoire des Interactions Plantes-Microorganismes (LIPM), INRA, UMR441, Chemin de Borde Rouge, F-31326, Castanet-Tolosan, France.|Laboratoire des Interactions Plantes-Microorganismes (LIPM), CNRS, UMR2594, Chemin de Borde Rouge, F-31326, Castanet-Tolosan, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Tremousaygue
authors.affiliation: Laboratoire des Interactions Plantes-Microorganismes (LIPM), INRA, UMR441, Chemin de Borde Rouge, F-31326, Castanet-Tolosan, France.|Laboratoire des Interactions Plantes-Microorganismes (LIPM), CNRS, UMR2594, Chemin de Borde Rouge, F-31326, Castanet-Tolosan, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Deslandes
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D060467:Disease Resistance; D005030:Ethylenes / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D043368:Ralstonia solanacearum / Q000472:pathogenicity*; D020156:Salicylic Acid / Q000378:metabolism; D015398:Signal Transduction; D014774:Virulence
chemical_list: D029681:Arabidopsis Proteins; C401621:CLV2 protein, Arabidopsis; D005030:Ethylenes; C000591832:MIRN169 microRNA, Arabidopsis; D008565:Membrane Proteins; D035683:MicroRNAs; C036216:ethylene; C118166:CLV1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases; D020156:Salicylic Acid
keywords: Affymetrix gene Chip; Arabidopsis thaliana; CLAVATA; NF-YA; bacterial wilt disease; miR169; plant susceptibility