title: Anti-MRSA and anti-TB metabolites from marine-derived Verrucosispora sp. MS100047.
abstract: Microbes belonging to the genus Verrucosispora possess significant chemical diversity and biological properties. They have attracted the interests of many researchers and are becoming promising resources in the marine natural product research field. A bioassay-guided isolation from the crude extract of Verrucosispora sp. strain MS100047, isolated from sediments collected from the South China Sea, has led to the identification of a new salicylic derivative, glycerol 1-hydroxy-2,5-dimethyl benzoate (1), along with three known compounds, brevianamide F (2), abyssomicin B (3), and proximicin B (4). Compound 1 showed selective activity against methicillin-resistant Staphylococcus aureus (MRSA) with a minimum inhibitory concentration (MIC) value of 12.5 μg/mL. Brevianamide F (2), which was isolated from actinomycete for the first time, showed a good anti-BCG activity with a MIC value of 12.5 μg/mL that has not been reported previously in literatures. Proximicin B (4) showed significant anti-MRSA (MIC = 3.125 μg/mL), anti-BCG (MIC = 6.25 μg/mL), and anti-tuberculosis (TB) (MIC = 25 μg/mL) activities. This is the first report on the anti-tubercular activities of proximicins. In addition, Verrucosispora sp. strain MS100047 was found to harbor 18 putative secondary metabolite gene clusters based on genomic sequence analysis. These include the biosynthetic loci encoding polyketide synthase (PKS) and non-ribosomal peptide synthetase (NRPS) consistent with abyssomicins and proximicins, respectively. The biosynthetic pathways of these isolated compounds have been proposed. These results indicate that MS100047 possesses a great potential as a source of active secondary metabolites.
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Huang
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.|School of Life Science, University of Science and Technology of China, Hefei, 230026, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Xie
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.|State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Ren
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.|Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, 71526, Egypt.
authors.forename: Wael M
authors.initials: WM
authors.lastname: Abdel-Mageed
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.|Eskitis Institute, Griffith University, Brisbane, QLD, 4111, Australia.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Liu
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Jianying
authors.initials: J
authors.lastname: Han
authors.affiliation: Forestry Research Institute of Nigeria, Jericho, Ibadan, Nigeria.
authors.forename: Ayokunmi
authors.initials: A
authors.lastname: Oyeleye
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Jinzhao
authors.initials: J
authors.lastname: Shen
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Fuhang
authors.initials: F
authors.lastname: Song
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Huanqin
authors.initials: H
authors.lastname: Dai
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. Liuxueting_cn@hotmail.com.
authors.forename: Xueting
authors.initials: X
authors.lastname: Liu
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. zhanglixin@im.ac.cn.
authors.forename: Lixin
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000995:Antitubercular Agents / Q000302:isolation & purification / Q000494:pharmacology*; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000302:isolation & purification / Q000494:pharmacology; D019015:Geologic Sediments / Q000382:microbiology; D005989:Glycerides / Q000302:isolation & purification / Q000494:pharmacology*; D026121:Indole Alkaloids / Q000302:isolation & purification / Q000494:pharmacology; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D008848:Micromonosporaceae / Q000378:metabolism*; D009163:Mycobacterium bovis / Q000187:drug effects*; D009429:Netropsin / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000494:pharmacology; D010453:Peptide Synthases / Q000235:genetics*; D048630:Polyketide Synthases / Q000235:genetics*; D012459:Salicylates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000995:Antitubercular Agents; D019086:Bridged Bicyclo Compounds, Heterocyclic; D005989:Glycerides; D026121:Indole Alkaloids; D012459:Salicylates; C000590722:brevianamide F; C000614445:glycerol 1-hydroxy-2,5-dimethyl benzoate; C529560:proximicin B; D009429:Netropsin; D048630:Polyketide Synthases; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase
keywords: Anti-BCG; Anti-MRSA; Marine-derived Verrucosispora; Salicylic derivative