title: Limited Dissemination of Extended-Spectrum β-Lactamase- and Plasmid-Encoded AmpC-Producing Escherichia coli from Food and Farm Animals, Sweden.
abstract: Extended-spectrum β-lactamase (ESBL)- and plasmid-encoded ampC (pAmpC)-producing Enterobacteriaceae might spread from farm animals to humans through food. However, most studies have been limited in number of isolates tested and areas studied. We examined genetic relatedness of 716 isolates from 4,854 samples collected from humans, farm animals, and foods in Sweden to determine whether foods and farm animals might act as reservoirs and dissemination routes for ESBL/pAmpC-producing Escherichia coli. Results showed that clonal spread to humans appears unlikely. However, we found limited dissemination of genes encoding ESBL/pAmpC and plasmids carrying these genes from foods and farm animals to healthy humans and patients. Poultry and chicken meat might be a reservoir and dissemination route to humans. Although we found no evidence of clonal spread of ESBL/pAmpC-producing E. coli from farm animals or foods to humans, ESBL/pAmpC-producing E. coli with identical genes and plasmids were present in farm animals, foods, and humans.
authors.forename: Stefan
authors.initials: S
authors.lastname: Börjesson
authors.forename: Sofia
authors.initials: S
authors.lastname: Ny
authors.forename: Maria
authors.initials: M
authors.lastname: Egervärn
authors.forename: Jakob
authors.initials: J
authors.lastname: Bergström
authors.forename: Åsa
authors.initials: Å
authors.lastname: Rosengren
authors.forename: Stina
authors.initials: S
authors.lastname: Englund
authors.forename: Sonja
authors.initials: S
authors.lastname: Löfmark
authors.forename: Sara
authors.initials: S
authors.lastname: Byfors
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000382:microbiology*; D016470:Bacteremia / Q000453:epidemiology* / Q000382:microbiology / Q000635:transmission; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002417:Cattle; D002645:Chickens / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000453:epidemiology* / Q000382:microbiology / Q000635:transmission; D005516:Food Microbiology; D015870:Gene Expression; D006801:Humans; D008460:Meat / Q000382:microbiology*; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D011201:Poultry Diseases / Q000453:epidemiology* / Q000382:microbiology / Q000635:transmission; D013548:Sweden / Q000453:epidemiology; D013552:Swine / Q000382:microbiology; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C048795:AmpC beta-lactamases; D001618:beta-Lactamases
keywords: ESBL; Escherichia coli; Sweden; antimicrobial resistance; bacteria; bloodstream infections; broilers; carriage; extended-spectrum cephalosporin; extended-spectrum β-lactamase; farm animals; foodborne infections; meat; national assessment; pAmpC; plasmid-encoded AmpC; poultry