title: [The role of oxidative protein modification and the gluthatione system in modulation of the redox status of breast epithelial cells].
abstract: The effects of the SH-group blocker N-ethylmaleimide (NEM) and thiol group protector 1,4-dithioerythritol (DTE) on the redox status of cells HBL-100 cells, oxidative modification of their proteins and the state of glutathione and thioredoxin systems have been investigated. Breast epithelial cells cultivated in the presence of NEM were characterized by decreased redox status, increased glutathione reductase activity, and increased concentrations of products of irreversible oxidative modification of protein and amino acids. Cultivation of HBL-100 cells in the presence of DTE resulted in a shift of the redox status towards reduction processes and increased reversible protein modification by glutathionylation. The proposed model of intracellular redox modulation may be used in the development of new therapeutic approaches to treat diseases accompanied by impaired redox homeostasis (e.g. oncologic, inflammatory, cardiovascular and neurodegenerative disease).
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Stepovaya
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Shakhristova
authors.affiliation: Siberian Federal University, Krasnoyarsk, Russia; Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Ryazantseva
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: O L
authors.initials: OL
authors.lastname: Nosareva
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: V D
authors.initials: VD
authors.lastname: Yakushina
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: A I
authors.initials: AI
authors.lastname: Nosova
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: V S
authors.initials: VS
authors.lastname: Gulaya
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Stepanova
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: R I
authors.initials: RI
authors.lastname: Chil'chigashev
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Novitsky
mesh_terms: D002460:Cell Line; D004226:Dithioerythritol / Q000494:pharmacology*; D004847:Epithelial Cells / Q000378:metabolism*; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D006801:Humans; D042361:Mammary Glands, Human / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D011499:Protein Processing, Post-Translational / Q000187:drug effects*
chemical_list: D004226:Dithioerythritol; D005978:Glutathione
keywords: breast epithelial cells; cell redox status; oxidative stress; protein oxidative modification