title: The effect of triiodothyronine on maturation and differentiation of oligodendrocyte progenitor cells during remyelination following induced demyelination in male albino rat.
abstract: Demyelination was induced by two weeks cuprizone treatment. Rats of +ve control and triiodothyronine (T3) then received three subcutaneous injections of either saline or T3 day after day and sacrificed at the end of the third and fifth weeks. Animals in -ve control group received only standard rodent chow. After one week of cuprizone withdrawal the corpus callosum in +ve control and T3 treated rats was still demyelinated as revealed by MBP immunohistochemistry. The assay of PLP gene showed significant increase of T3 treated group compared to both the -ve control and +ve control groups. After three weeks, significant improvement in myelination was detected in T3-treated group compared to +ve control as detected by both MBP immunohistochemistry and electron microscopy. After one week of cuprizone withdrawal, PDGFRα positive cells and gene expression showed significant increase in +ve control and T3-treated groups as compared to -ve control with insignificant difference in between the former two groups. After three weeks of cuprizone withdrawal, PDGFRα positive cells in T3-treated and +ve control groups decreased to the control levels. These results suggest that T3 was effective in improving remyelination when administered during acute phase and might direct progenitor lineage toward oligodendrocytes.
authors.affiliation: Anatomy Department, Faculty of Medicine, Mansoura University, Egypt.
authors.forename: H
authors.initials: H
authors.lastname: El-Tahry
authors.forename: Hany E
authors.initials: HE
authors.lastname: Marei
authors.affiliation: Anatomy Department, Faculty of Medicine, Mansoura University, Egypt.
authors.forename: A
authors.initials: A
authors.lastname: Shams
authors.affiliation: Anatomy Department, Faculty of Medicine, Mansoura University, Egypt. Electronic address: mshahat50@gmail.com.
authors.forename: M
authors.initials: M
authors.lastname: El-Shahat
authors.affiliation: Medical Biochemistry Department, Faculty of Medicine, Mansoura University, Egypt.
authors.forename: H
authors.initials: H
authors.lastname: Abdelaziz
authors.affiliation: Anatomy Department, Faculty of Medicine, Mansoura University, Egypt.
authors.forename: M
authors.initials: M
authors.lastname: Abd El-Kader
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D003337:Corpus Callosum / Q000187:drug effects / Q000648:ultrastructure; D003471:Cuprizone / Q000008:administration & dosage; D003711:Demyelinating Diseases / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D007279:Injections, Subcutaneous; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D009836:Oligodendroglia / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000096:biosynthesis; D013234:Stem Cells / Q000187:drug effects* / Q000378:metabolism; D014284:Triiodothyronine / Q000008:administration & dosage*
chemical_list: D014284:Triiodothyronine; D003471:Cuprizone; D020796:Receptor, Platelet-Derived Growth Factor alpha
keywords: Cuprizone; Oligodendrocyte progenitor cells; Remyelination; Triiodothyronine