title: UFLC-MS/MS determination and pharmacokinetic studies of six Saikosaponins in rat plasma after oral administration of Bupleurum Dropping Pills.
abstract: A rapid and sensitive ultra fast liquid chromatography tandem mass spectrometry method (UFLC-MS/MS) was developed and validated for the simultaneous determination of six Saikosaponins (SSs) (SSa, SSb1, SSb2, SSd, SSc, SSf) of Bupleurum Dropping Pills (BDP) in rat plasma using chloramphenicol as the internal standard (IS). The SSs were separated using an ACQUITY UPLC(®) BEH C18 column (50 mm × 2.1mm, 1.7 μm) and detection of these compounds were done by using a Qtrap 5500 mass spectrometer coupled with negative electrospray ionization (ESI) source under the multiple reaction monitoring (MRM) mode. According to regulatory guidelines, the established method was fully validated and results were showed within acceptable limits. The lower limit of quantifications (LLOQs) of all analytes were 0.2 ng/mL. The validated method was successfully applied into a pharmacokinetic study of orally administered BDP in rats.
authors.affiliation: Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, China; Tasly Academy, Tianjin Tasly Group Co., Ltd., Tianjin 300410, China.
authors.forename: Xiufeng
authors.initials: X
authors.lastname: Guan
authors.affiliation: Tasly Academy, Tianjin Tasly Group Co., Ltd., Tianjin 300410, China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Tasly Academy, Tianjin Tasly Group Co., Ltd., Tianjin 300410, China.
authors.forename: Kaijing
authors.initials: K
authors.lastname: Yan
authors.affiliation: Tasly Academy, Tianjin Tasly Group Co., Ltd., Tianjin 300410, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chu
authors.affiliation: Tasly Academy, Tianjin Tasly Group Co., Ltd., Tianjin 300410, China.
authors.forename: Shuming
authors.initials: S
authors.lastname: Li
authors.affiliation: Tasly Academy, Tianjin Tasly Group Co., Ltd., Tianjin 300410, China; State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. Electronic address: liwei_wzhy@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, China. Electronic address: yanxueying00@163.com.
authors.forename: Xueying
authors.initials: X
authors.lastname: Yan
authors.affiliation: Tasly Academy, Tianjin Tasly Group Co., Ltd., Tianjin 300410, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Ma
authors.affiliation: Tasly Academy, Tianjin Tasly Group Co., Ltd., Tianjin 300410, China.
authors.forename: Shuiping
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Tasly Academy, Tianjin Tasly Group Co., Ltd., Tianjin 300410, China; Tasly Pharmaceuticals Inc., Rockvill, MD 20850, USA.
authors.forename: He
authors.initials: H
authors.lastname: Sun
authors.affiliation: Tianjin State Key Laboratory of Pharmacokinetics and Pharmacodynamics, Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China.
authors.forename: Changxiao
authors.initials: C
authors.lastname: Liu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D029972:Bupleurum / Q000737:chemistry*; D057230:Limit of Detection; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D010936:Plant Extracts / Q000008:administration & dosage*; D051381:Rats; D012015:Reference Standards; D012503:Saponins / Q000097:blood / Q000493:pharmacokinetics*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D010936:Plant Extracts; D012503:Saponins; D009828:Oleanolic Acid; C025759:saikosaponin D
keywords: Bupleurum dropping pills; Pharmacokinetics; Saikosaponin; UFLC–MS/MS