title: Sonic hedgehog is a chemotactic neural crest cell guide that is perturbed by ethanol exposure.
abstract: Our aim was to understand the involvement of Sonic hedgehog (Shh) morphogen in the oriented distribution of neural crest cells (NCCs) toward the optic vesicle and to look for potential disorders of this guiding mechanism after ethanol exposure. In vitro directional analysis showed the chemotactic response of NCCs up Shh gradients and to notochord co-cultures (Shh source) or to their conditioned medium, a response inhibited by anti-Shh antibody, receptor inhibitor cyclopamine and anti-Smo morpholino (MO). Expression of the Ptch-Smo receptor complex on in vitro NCCs was also shown. In whole embryos, the expression of Shh mRNA and protein was seen in the ocular region, and of Ptch, Smo and Gli/Sufu system on cephalic NCCs. Anti-Smo MO or Ptch-mutated plasmid (Ptch1(Δloop2)) impaired cephalic NCC migration/distribution, with fewer cells invading the optic region and with higher cell density at the homolateral mesencephalic level. Beads embedded with cyclopamine (Smo-blocking) or Shh (ectopic signal) supported the role of Shh as an in vivo guide molecule for cephalic NCCs. Ethanol exposure perturbed in vitro and in vivo NCC migration. Early stage embryos treated with ethanol, in a model reproducing Fetal Alcohol Syndrome, showed later disruptions of craniofacial development associated with abnormal in situ expression of Shh morphogen. The results show the Shh/Ptch/Smo-dependent migration of NCCs toward the optic vesicle, with the support of specific inactivation with genetic and pharmacological tools. They also help to understand mechanisms of accurate distribution of embryonic cells and of their perturbation by a commonly consumed teratogen, and demonstrate, in addition to its other known developmental functions, a new biological activity of cellular guidance for Shh.
authors.affiliation: Center for Cellular and Molecular Biology, CEBICEM-IIBYT (UNC-CONICET), National University of Córdoba, Av. Vélez Sarsfield 1611, 5016 Córdoba, Argentina; Schulze Center for Novel Therapeutics, División of Oncology Research, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Ezequiel J
authors.initials: EJ
authors.lastname: Tolosa
authors.affiliation: Schulze Center for Novel Therapeutics, División of Oncology Research, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Martín E
authors.initials: ME
authors.lastname: Fernández-Zapico
authors.affiliation: Center for Cellular and Molecular Biology, CEBICEM-IIBYT (UNC-CONICET), National University of Córdoba, Av. Vélez Sarsfield 1611, 5016 Córdoba, Argentina.
authors.forename: Natalia L
authors.initials: NL
authors.lastname: Battiato
authors.affiliation: Center for Cellular and Molecular Biology, CEBICEM-IIBYT (UNC-CONICET), National University of Córdoba, Av. Vélez Sarsfield 1611, 5016 Córdoba, Argentina. Electronic address: rrovasio@yahoo.com.ar.
authors.forename: Roberto A
authors.initials: RA
authors.lastname: Rovasio
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D002633:Chemotaxis / Q000187:drug effects*; D002645:Chickens; D000431:Ethanol / Q000494:pharmacology*; D053823:Hedgehog Proteins / Q000378:metabolism*; D009432:Neural Crest / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism
chemical_list: D053823:Hedgehog Proteins; D000431:Ethanol
keywords: Cell guiding; Cell migration; Chemotaxis; Chick embryo; Ethanol; Neural crest cells; Sonic hedgehog