title: Esterase mediated resistance in deltamethrin resistant reference tick colony of Rhipicephalus (Boophilus) microplus.
abstract: Monitoring of acaricide resistance is considered as one of the important facets of integrated tick management. In an attempt of development of resistance monitoring indicators, in the present study two reference tick lines of Rhipicephalus (Boophilus) microplus maintained in the Entomology laboratory, Indian Veterinary Research Institute (IVRI), Izatnagar, India, were studied to determine the possible contributing factors involved in development of resistance to deltamethrin. Electrophoretic profiling of esterase enzymes detected high activities of EST-1 in reference resistant tick colony designated as IVRI-IV whereas it was not detectable in reference susceptible IVRI-I line of R. (B.) microplus. Esterases were further characterized as carboxylesterase or acetylcholinesterase based on inhibitor study using PMSF, eserine sulphate, malathion, TPP and copper sulphate. It was concluded that an acetylcholinesterase, EST-1, possibly plays an important role for development of deltamethrin resistance in IVRI-IV colony of R. (B.) microplus.
authors.affiliation: Entomology Laboratory, Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., 243122, India.
authors.forename: Snehil
authors.initials: S
authors.lastname: Gupta
authors.affiliation: Entomology Laboratory, Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., 243122, India.
authors.forename: K G
authors.initials: KG
authors.lastname: Ajith Kumar
authors.affiliation: Entomology Laboratory, Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., 243122, India.
authors.forename: Anil Kumar
authors.initials: AK
authors.lastname: Sharma
authors.affiliation: Entomology Laboratory, Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., 243122, India.
authors.forename: Gaurav
authors.initials: G
authors.lastname: Nagar
authors.affiliation: Entomology Laboratory, Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., 243122, India.
authors.forename: Sachin
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Entomology Laboratory, Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., 243122, India.
authors.forename: B C
authors.initials: BC
authors.lastname: Saravanan
authors.affiliation: Entomology Laboratory, Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., 243122, India.
authors.forename: Gandham
authors.initials: G
authors.lastname: Ravikumar
authors.affiliation: Entomology Laboratory, Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., 243122, India. sghoshp@yahoo.co.in.
authors.forename: Srikant
authors.initials: S
authors.lastname: Ghosh
mesh_terms: D056810:Acaricides / Q000494:pharmacology*; D000818:Animals; D060829:Arthropod Proteins / Q000378:metabolism*; D004351:Drug Resistance; D004950:Esterases / Q000378:metabolism*; D007194:India; D007814:Larva / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000502:physiology; D009570:Nitriles / Q000494:pharmacology*; D011722:Pyrethrins / Q000494:pharmacology*; D048494:Rhipicephalus / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development / Q000502:physiology
chemical_list: D056810:Acaricides; D060829:Arthropod Proteins; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin; D004950:Esterases
keywords: Esterases; IVRI-IV tick colony; India; Resistance; Rhipicephalus (Boophilus) microplus