title: Comparative Proteome Analysis of the Tuberous Roots of Six Cassava (Manihot esculenta) Varieties Reveals Proteins Related to Phenotypic Traits.
abstract: Cassava (Manihot esculenta Crantz) is a staple food and an important source of starch, and the attributes of its tuberous root largely depend on the variety. The proteome of cassava has been investigated; however, to date, no study has focused on varieties that reveal the molecular basis of phenotypical characteristics. Therefore, we aimed to compare the proteome of the tuberous roots of six cassava varieties that differed in carbohydrates, carotenoids, and resistance to diseases, among other attributes. Two-dimensional gels showed 146 differential spots between the varieties, and the functional roles of some differential proteins were correlated to phenotypic characteristics of the varieties, such as the amount of carbohydrates or carotenoids and the resistance to biotic or abiotic stresses. The results obtained here highlight elements that might help to direct the improvement of new cultivars of cassava, which is an economically and socially relevant crop worldwide.
authors.affiliation: Department of Food Science and Experimental Nutrition, FCF, University of São Paulo , São Paulo, Brazil.
authors.forename: Gabriela Justamante Händel
authors.initials: GJ
authors.lastname: Schmitz
authors.affiliation: Department of Food Science, University of Guelph , Guelph, Canada.
authors.forename: Jonathan
authors.initials: J
authors.lastname: de Magalhães Andrade
authors.affiliation: Agronomic Institute of the State of São Paulo , Campinas, Brazil.
authors.forename: Teresa Losada
authors.initials: TL
authors.lastname: Valle
authors.affiliation: Department of Genetics, University of São Paulo , Piracicaba, Brazil.
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: Labate
authors.affiliation: Department of Food Science and Experimental Nutrition, FCF, University of São Paulo , São Paulo, Brazil.|Food and Nutrition Research Center (NAPAN), University of São Paulo , São Paulo, Brazil.|Food Research Center (FoRC-CEPID) , São Paulo, Brazil.
authors.forename: João Roberto Oliveira
authors.initials: JR
authors.lastname: do Nascimento
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D002365:Manihot / Q000145:classification / Q000378:metabolism*; D010641:Phenotype; D010940:Plant Proteins / Q000378:metabolism*; D020543:Proteome; D013045:Species Specificity
chemical_list: D010940:Plant Proteins; D020543:Proteome
keywords: Manihot esculenta Crantz; bitter cassava; proteomic analysis; sweet cassava; tuberous roots