title: Validation, optimisation, and application data in support of the development of a targeted selected ion monitoring assay for degraded cardiac troponin T.
abstract: Cardiac troponin T (cTnT) fragmentation in human serum was investigated using a newly developed targeted selected ion monitoring assay, as described in the accompanying article: "Development of a targeted selected ion monitoring assay for the elucidation of protease induced structural changes in cardiac troponin T" [1]. This article presents data describing aspects of the validation and optimisation of this assay. The data consists of several figures, an excel file containing the results of a sequence identity search, and a description of the raw mass spectrometry (MS) data files, deposited in the ProteomeXchange repository with id PRIDE: PXD003187.
authors.affiliation: Central Diagnostic Laboratory, Maastricht University Medical Centre, Maastricht, the Netherlands.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Streng
authors.affiliation: Central Diagnostic Laboratory, Maastricht University Medical Centre, Maastricht, the Netherlands.
authors.forename: Douwe
authors.initials: D
authors.lastname: de Boer
authors.affiliation: Department of Human Biology, Maastricht University, Maastricht, the Netherlands.
authors.forename: Freek G
authors.initials: FG
authors.lastname: Bouwman
authors.affiliation: Department of Human Biology, Maastricht University, Maastricht, the Netherlands.
authors.forename: Edwin C M
authors.initials: EC
authors.lastname: Mariman
authors.affiliation: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands; Netherlands Proteomics Centre, Utrecht University, Utrecht, the Netherlands; Current working address: Janssen, Pharmaceutical Companies of Johnson&Johnson, Infectious Diseases and Vaccines, Leiden, the Netherlands.
authors.forename: Arjen
authors.initials: A
authors.lastname: Scholten
authors.affiliation: Central Diagnostic Laboratory, Maastricht University Medical Centre, Maastricht, the Netherlands.
authors.forename: Marja P
authors.initials: MP
authors.lastname: van Dieijen-Visser
authors.affiliation: Central Diagnostic Laboratory, Maastricht University Medical Centre, Maastricht, the Netherlands.
authors.forename: Will K W H
authors.initials: WK
authors.lastname: Wodzig