title: Highly Branched Pentasaccharide-Bearing Amphiphiles for Membrane Protein Studies.
abstract: Detergents are essential tools for membrane protein manipulation. Micelles formed by detergent molecules have the ability to encapsulate the hydrophobic domains of membrane proteins. The resulting protein-detergent complexes (PDCs) are compatible with the polar environments of aqueous media, making structural and functional analysis feasible. Although a number of novel agents have been developed to overcome the limitations of conventional detergents, most have traditional head groups such as glucoside or maltoside. In this study, we introduce a class of amphiphiles, the PSA/Es with a novel highly branched pentasaccharide hydrophilic group. The PSA/Es conferred markedly increased stability to a diverse range of membrane proteins compared to conventional detergents, indicating a positive role for the new hydrophilic group in maintaining the native protein integrity. In addition, PDCs formed by PSA/Es were smaller and more suitable for electron microscopic analysis than those formed by DDM, indicating that the new agents have significant potential for the structure-function studies of membrane proteins.
authors.affiliation: Department of Bionanotechnology, Hanyang University , Ansan, 426-791, Korea.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ehsan
authors.affiliation: Molecular and Cellular Physiology, Stanford University , Stanford, California 94305, United States.
authors.forename: Yang
authors.initials: Y
authors.lastname: Du
authors.affiliation: Department of Life Sciences, Imperial College London , London, SW7 2AZ, U.K.
authors.forename: Nicola J
authors.initials: NJ
authors.lastname: Scull
authors.affiliation: Department of Cell Physiology and Molecular Biophysics, Center for Membrane Protein Research, School of Medicine, Texas Tech University Health Sciences Center , Lubbock, Texas 79430, United States.
authors.forename: Elena
authors.initials: E
authors.lastname: Tikhonova
authors.affiliation: Life Sciences Institute, University of Michigan , Ann Arbor, Michigan 48109, United States.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Tarrasch
authors.affiliation: Department of Neuroscience and Pharmacology, University of Copenhagen , Copenhagen, DK-2200, Denmark.
authors.forename: Jonas S
authors.initials: JS
authors.lastname: Mortensen
authors.affiliation: Department of Neuroscience and Pharmacology, University of Copenhagen , Copenhagen, DK-2200, Denmark.
authors.forename: Claus J
authors.initials: CJ
authors.lastname: Loland
authors.affiliation: Life Sciences Institute, University of Michigan , Ann Arbor, Michigan 48109, United States.
authors.forename: Georgios
authors.initials: G
authors.lastname: Skiniotis
authors.affiliation: Department of Cell Physiology and Molecular Biophysics, Center for Membrane Protein Research, School of Medicine, Texas Tech University Health Sciences Center , Lubbock, Texas 79430, United States.
authors.forename: Lan
authors.initials: L
authors.lastname: Guan
authors.affiliation: Department of Life Sciences, Imperial College London , London, SW7 2AZ, U.K.
authors.forename: Bernadette
authors.initials: B
authors.lastname: Byrne
authors.affiliation: Molecular and Cellular Physiology, Stanford University , Stanford, California 94305, United States.
authors.forename: Brian K
authors.initials: BK
authors.lastname: Kobilka
authors.affiliation: Department of Bionanotechnology, Hanyang University , Ansan, 426-791, Korea.
authors.forename: Pil Seok
authors.initials: PS
authors.lastname: Chae
mesh_terms: D017920:Antiporters / Q000032:analysis / Q000737:chemistry; D029681:Arabidopsis Proteins / Q000032:analysis / Q000737:chemistry; D002240:Carbohydrate Sequence; D003902:Detergents / Q000138:chemical synthesis / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008565:Membrane Proteins / Q000032:analysis / Q000737:chemistry*; D008823:Micelles; D008958:Models, Molecular; D025942:Oligosaccharides, Branched-Chain / Q000138:chemical synthesis / Q000737:chemistry*; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship
chemical_list: D017920:Antiporters; D029681:Arabidopsis Proteins; C469216:BOR1 protein, Arabidopsis; D003902:Detergents; D008565:Membrane Proteins; D008823:Micelles; D025942:Oligosaccharides, Branched-Chain; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins