title: [Epidemiology of caprine and ovine brucellosis in Formosa province, Argentina].
abstract: An epidemiological study of brucellosis was carried out in 516 goats and mixed flocks (goat/sheep) from the three agro-ecological regions of Formosa province, Argentina. Serum samples from a total of 25401 goats and 2453 sheeps were analyzed using buffered plate agglutination test (BPAT) and complement fixation test (CFT). Bacteriological and PCR analyses on milk samples from goats in three flocks with a history of brucellosis and recent abortions were also performed. Brucellosis was detected in four of the nine departments of the province with an overall prevalence of 2% and an intra-flock prevalence ranging between 1% and 40%. The proportion of infected flocks was 3.6%, 12% and 36% for the eastern, central and western regions, respectively. Brucella melitensis bv. 1 was isolated efrom goats for the first time in the province. The expected fragments of 827bp from the omp2ab gene (Brucella spp.) and 731bp from the insert IS711 (B. melitensis) were amplified by PCR. Detection of antibodies by BPAT and FCT in sheep cohabiting with goats suggests that infections could have been caused by B. melitensis, posing an additional risk to public health. Control and eradication programs for brucellosis should consider mixed flocks as a single epidemiological unit. The results indicate that brucellosis by B. melitensis bv1 is highly endemic in the central and western regions of Formosa province.
authors.affiliation: Centro de Investigaciones y Transferencia-CIT Formosa (CONICET-Universidad Nacional de Formosa-Gobierno de la Provincia de Formosa), Formosa, Argentina. Electronic address: anabordoy@hotmail.com.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Russo
authors.affiliation: Centro de Investigaciones y Transferencia-CIT Formosa (CONICET-Universidad Nacional de Formosa-Gobierno de la Provincia de Formosa), Formosa, Argentina.
authors.forename: Orlando A
authors.initials: OA
authors.lastname: Mancebo
authors.affiliation: Centro de Investigaciones y Transferencia-CIT Formosa (CONICET-Universidad Nacional de Formosa-Gobierno de la Provincia de Formosa), Formosa, Argentina.
authors.forename: Carlos M
authors.initials: CM
authors.lastname: Monzón
authors.affiliation: Ministerio de la Producción y Ambiente de la Provincia de Formosa, Formosa, Argentina.
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Gait
authors.affiliation: Ministerio de la Producción y Ambiente de la Provincia de Formosa, Formosa, Argentina.
authors.forename: Rubén D
authors.initials: RD
authors.lastname: Casco
authors.affiliation: Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Rafaela, Laboratorio de Inmunología, Rafaela, Santa Fe, Argentina.
authors.forename: Susana M
authors.initials: SM
authors.lastname: Torioni de Echaide
mesh_terms: D000034:Abortion, Veterinary / Q000209:etiology / Q000382:microbiology; D000822:Animal Husbandry / Q000379:methods; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001118:Argentina / Q000453:epidemiology; D015373:Bacterial Typing Techniques; D017347:Brucella melitensis / Q000276:immunology / Q000302:isolation & purification*; D002006:Brucellosis / Q000453:epidemiology / Q000382:microbiology / Q000635:transmission / Q000662:veterinary*; D005260:Female; D062306:Geography, Medical; D015511:Goat Diseases / Q000453:epidemiology* / Q000382:microbiology / Q000635:transmission; D006041:Goats / Q000382:microbiology; D008297:Male; D008892:Milk / Q000382:microbiology; D011247:Pregnancy; D015995:Prevalence; D016036:Seroepidemiologic Studies; D012756:Sheep / Q000382:microbiology; D012757:Sheep Diseases / Q000453:epidemiology* / Q000382:microbiology / Q000635:transmission
chemical_list: D000907:Antibodies, Bacterial
keywords: Argentina; Brucella melitensis bv 1; Brucellosis; Brucelosis; CFT melitensis bv. 1; Caprinos; Formosa; Goats; Ovinos; Sheep