title: Comprehensive two-dimensional liquid chromatographic analysis of poloxamers.
abstract: Poloxamers are low molar mass triblock copolymers of poly(ethylene oxide) (PEO) and poly(propylene oxide) (PPO), having number of applications as non-ionic surfactants. Comprehensive one and two-dimensional liquid chromatographic (LC) analysis of these materials is proposed in this study. The separation of oligomers of both types (PEO and PPO) is demonstrated for several commercial poloxamers. This is accomplished at the critical conditions for one of the block while interaction for the other block. Reversed phase LC at CAP of PEO allowed for oligomeric separation of triblock copolymers with regard to PPO block whereas normal phase LC at CAP of PPO renders oligomeric separation with respect to PEO block. The oligomeric separation with regard to PEO and PPO are coupled online (comprehensive 2D-LC) to reveal two-dimensional contour plots by unconventional 2D IC×IC (interaction chromatography) coupling. The study provides chemical composition mapping of both PEO and PPO, equivalent to combined molar mass and chemical composition mapping for several commercial poloxamers.
authors.affiliation: Division of Advanced Materials Science and Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea; H.E.J. Research Institute of Chemistry, International Centre for Chemical and Biological Sciences (ICCBS), University of Karachi, Karachi 75270, Pakistan. Electronic address: mimran.malik@iccs.edu.
authors.forename: Muhammad Imran
authors.initials: MI
authors.lastname: Malik
authors.affiliation: Division of Advanced Materials Science and Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea.
authors.forename: Sanghoon
authors.initials: S
authors.lastname: Lee
authors.affiliation: Division of Advanced Materials Science and Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea. Electronic address: tc@postech.ac.kr.
authors.forename: Taihyun
authors.initials: T
authors.lastname: Chang
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002853:Chromatography, Liquid; D008970:Molecular Weight; D020442:Poloxamer / Q000032:analysis*; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011409:Propylene Glycols / Q000737:chemistry
chemical_list: D011108:Polymers; D011409:Propylene Glycols; D020442:Poloxamer; C012504:polypropylene glycol; D011092:Polyethylene Glycols
keywords: IC–IC coupling; Interaction chromatography (IC); Liquid chromatography at critical conditions; Poloxamers; Two-dimensional liquid chromatography