title: Single Nucleotide Variant rs2232710 in the Protein Z-Dependent Protease Inhibitor (ZPI, SERPINA10) Gene Is Not Associated with Deep Vein Thrombosis.
abstract: Rare mutations in PROC, PROS1 or SERPINC1 as well as common variants in F5, F2, F11 and SERPINC1 have been identified as risk factors for deep vein thrombosis (DVT). To identify novel genetic risk factors for DVT, we have developed and applied next-generation DNA sequencing (NGS) of the coding area of hemostatic and proinflammatory genes. Using this strategy, we previously identified a single nucleotide variant (SNV) rs6050 in the FGA gene and novel, rare SNVs in the ADAMTS13 gene associated with DVT. To identify novel coding variants in the genetic predisposition to DVT, we applied NGS analysis of the coding area of 186 hemostatic and proinflammatory genes in 94 DVT cases and 98 controls and we identified 18 variants with putative role in DVT. A group of 585 Italian idiopathic DVT patients and 550 healthy controls was used to genotype all the 18 risk-associated variants identified by NGS. Replication study in the Italian population identified the rs2232710 variant in the protein Z-dependent protease inhibitor (ZPI) gene to be associated with an increased risk of DVT (OR 2.74; 95% CI 1.33-5.65; P = 0.0045; Bonferroni P = 0.081). However, the rs2232710 SNV showed no association with DVT in two Dutch replication cohorts the LETS study (454 patients and 451 controls) and the MEGA study (3799 patients and 4399 controls), indicating that the rs2232710 variant is not a risk factor for DVT.
authors.affiliation: Department of Pathophysiology and Transplantation, Università degli Studi di Milano, Milan, Italy.
authors.forename: Marcin M
authors.initials: MM
authors.lastname: Gorski
authors.affiliation: Angelo Bianchi Bonomi Hemophilia and Thrombosis Center, Fondazione IRCCS Cà Granda - Ospedale Maggiore Policlinico and Fondazione Luigi Villa, Milan, Italy.
authors.forename: Luca A
authors.initials: LA
authors.lastname: Lotta
authors.affiliation: Department of Pathophysiology and Transplantation, Università degli Studi di Milano, Milan, Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Pappalardo
authors.affiliation: Departments of Clinical Epidemiology, Leiden University Medical Center, Leiden, Netherlands.
authors.forename: Hugoline G
authors.initials: HG
authors.lastname: de Haan
authors.affiliation: Angelo Bianchi Bonomi Hemophilia and Thrombosis Center, Fondazione IRCCS Cà Granda - Ospedale Maggiore Policlinico and Fondazione Luigi Villa, Milan, Italy.
authors.forename: Serena M
authors.initials: SM
authors.lastname: Passamonti
authors.affiliation: Departments of Clinical Epidemiology, Leiden University Medical Center, Leiden, Netherlands.
authors.forename: Astrid
authors.initials: A
authors.lastname: van Hylckama Vlieg
authors.affiliation: Angelo Bianchi Bonomi Hemophilia and Thrombosis Center, Fondazione IRCCS Cà Granda - Ospedale Maggiore Policlinico and Fondazione Luigi Villa, Milan, Italy.
authors.forename: Ida
authors.initials: I
authors.lastname: Martinelli
authors.affiliation: Department of Pathophysiology and Transplantation, Università degli Studi di Milano, Milan, Italy.|Angelo Bianchi Bonomi Hemophilia and Thrombosis Center, Fondazione IRCCS Cà Granda - Ospedale Maggiore Policlinico and Fondazione Luigi Villa, Milan, Italy.
authors.forename: Flora
authors.initials: F
authors.lastname: Peyvandi
mesh_terms: D016022:Case-Control Studies; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D015843:Serpins / Q000235:genetics*; D020246:Venous Thrombosis / Q000235:genetics*
chemical_list: C416370:SERPINA10 protein, human; D015843:Serpins