title: Overexpression of tissue microRNA10b may help predict glioma prognosis.
abstract: We investigated the relationship between microRNA-10b (miR-10b) expression and prognosis in human glioma patients. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis was used to characterize the expression patterns of miR-10b in 128 glioma and 20 normal brain tissues. Clinical information - age, sex, Karnofsky Performance Status (KPS) and World Health Organization (WHO) grade - were also collected. The associations between miR-10b expression and the clinicopathological factors and outcome of glioma patients were statistically analyzed. Expression levels of miR-10b in glioma tissue were significantly higher than in normal brain tissue (P<0.001). High-grade glioma (WHO grade III and IV) had much higher miR-10b expression levels than low-grade tumors (WHO grade I and II). Additionally, the increased miR-10b expression in the glioma tissues was significantly associated with a low KPS (P=0.03). Kaplan-Meier survival curves and Cox regression analyses showed that overexpression of miR-10b (P=0.01) and high grade (P=0.02) were independent factors predicting poor outcome for glioma patients. Furthermore, subgroup analyses showed that the miR-10b expression level was significantly associated with poor overall survival in glioma patients with high grades (P<0.001). Up-regulation of miR-10b may have value in predicting clinical outcome in glioma patients, particularly for those with high pathological grades.
authors.affiliation: Shandong Cancer Hospital and Institute, 440 Jiyan, Jinan 250117, Shandong Province, China.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: The Second Hospital of Shandong University, Shandong Province, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Affiliated Hospital of Weifang Medical University, Weifang, Shandong Province, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Fu
authors.affiliation: Shandong Cancer Hospital and Institute, 440 Jiyan, Jinan 250117, Shandong Province, China. Electronic address: qingshui20150507@sina.com.
authors.forename: Qingshui
authors.initials: Q
authors.lastname: Li
mesh_terms: D000359:Aftercare; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001932:Brain Neoplasms / Q000378:metabolism* / Q000601:surgery; D005260:Female; D005910:Glioma / Q000378:metabolism* / Q000601:surgery; D006801:Humans; D053208:Kaplan-Meier Estimate; D017567:Karnofsky Performance Status; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D060787:Neoplasm Grading; D017063:Outcome Assessment, Health Care; D011379:Prognosis; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C547183:MIRN10 microRNA, human; D035683:MicroRNAs
keywords: Glioma; Prognosis; RT-PCR; miR-10b; microRNA-10b