title: [Effect of 2-methacryloyloxyethyl phosphorylcholine on the protein-repellent property of dental adhesive].
abstract: OBJECTIVE
  To evaluate the effect of 2-methacryloyloxyethyl phosphorylcholine (MPC) and nanoparticles of amorphous calcium phosphate (NACP) on the protein-repellent property of dental adhesive.
  
  
  METHODS
  MPC and NACP were incorporated into SBMP as the test group. Scotchbond Multi-Purpose (SBMP) was used as control group. Human dentin shear bond strengths were measured. Protein adsorption onto samples was determined by micro bicinchoninic acid (BCA) method. A dental plaque microcosm biofilm model with human saliva as inoculum was used to investigate biofilm viability.
  
  
  RESULTS
  The dentin bond strength of modified group was (28.7±2.2) MPa, which was not significantly different from that of the SBMP control group. The amount of protein adsorption in the modified group and the SBMP control group were (0.21±0.02) µg/cm(2) and (4.17±0.45) µg/cm(2) respectively. Lactic acid production of biofilms in modified group and SBMP control were (7.71 ± 1.01) mmol/L and (19.18 ± 2.34) mmol/L repectively.
  
  
  CONCLUSIONS
  MPC-NACP based dental adhesive greatly reduce the protein adsorption and bacterial adhesion, without compromising dentin shear bond strength. This novel bonding agent may have wide application.
authors.affiliation: Department of Orthodontics, Capital Medical University School of Stomatology, Beijing 100050, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Department of Orthodontics, Capital Medical University School of Stomatology, Beijing 100050, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Biomaterials & Tissue Engineering Division, University of Maryland Dental School, Baltimore, MD 21201, U S A.
authors.forename: Huakun
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Orthodontics, Capital Medical University School of Stomatology, Beijing 100050, China.
authors.forename: Yuxing
authors.initials: Y
authors.lastname: Bai
mesh_terms: D000327:Adsorption; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D002130:Calcium Phosphates / Q000494:pharmacology*; D003738:Dental Cements / Q000494:pharmacology*; D003773:Dental Plaque; D003804:Dentin / Q000737:chemistry; D006801:Humans; D019344:Lactic Acid / Q000096:biosynthesis; D008689:Methacrylates / Q000494:pharmacology*; D053758:Nanoparticles; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000494:pharmacology; D019279:Resin Cements / Q000494:pharmacology; D012463:Saliva; D013718:Tensile Strength
chemical_list: D002130:Calcium Phosphates; D003738:Dental Cements; D008689:Methacrylates; D019279:Resin Cements; C084018:Scotchbond Multi-Purpose; C519480:amorphous calcium phosphate; D010767:Phosphorylcholine; D019344:Lactic Acid; C070638:2-methacryloyloxyethyl phosphorylcholine