title: [Preliminary results of femtosecond laser-assisted Descemet stripping endothelial keratoplasty for bullous keratopathy].
abstract: OBJECTIVE
  To evaluate the preliminary results of femtosecond laser-assisted Descemet stripping endothelial keratoplasty (FS-DSEK) for the treatment of bullous keratopathy.
  
  
  METHODS
  In the nonrandomized, prospective, consecutive case series, 7 eyes with bullous keratopathy induced by cataract surgery received FS-DSEK surgery between September and December 2013. Standard Descemet stripping endothelial keratoplasty procedure was performed. The Descemet membrane and abnormal endothelial layer were stripped from the central recipient posterior surface in a diameter of 7.75 mm. The graft was fixed to the edge of recipient cornea with one single stitch. The donor lenticule was produced by Intralase 150 femtosecond laser with a diameter of 7.75 mm. The adherence of donor lenticule to the recipient posterior stroma and postoperative donor lenticule dislocation were monitored in the early stage after surgery. Best spectacle-corrected visual acuity, refraction, endothelial cell density and thickness of grafts were measured preoperatively, and at 1 week, 1, 3, 6 and 12 months after FS-DSEK.
  
  
  RESULTS
  Two eyes had graft dislocation, with a gap between the graft and recipent posterior stroma at 2 days after surgery. The grafts were repositioned after bubbles were added into the anterior chamber. All grafts were well adherent to the recipent posterior stromal surface at 1 week postoperatively. One graft failed at 3 months postoperatively, and penetrating keratoplasty was performed. At 12 months, the best spectacle-corrected visual acuity was 0.04 to 0.7, the mean endothelial cell density was (1 698.0±251.8) cells/mm(2), and the mean thickness of grafts was (124.2 ± 11.4) μm.
  
  
  CONCLUSIONS
  Femtosecond laser-assisted Descemet stripping endothelial keratoplasty was effective in treating bullous keratopathy. Stitching the graft to the recipent cornea can prevent postoperative graft dislocation effectively.
authors.affiliation: The Third Affiliated Hospital of Nanchang University, Nanchang 330008, China.
authors.forename: G F
authors.initials: GF
authors.lastname: Huang
authors.forename: Y
authors.initials: Y
authors.lastname: Gao
authors.forename: X J
authors.initials: XJ
authors.lastname: Charlotte Aimee
authors.forename: G B
authors.initials: GB
authors.lastname: Liao
authors.forename: Y J
authors.initials: YJ
authors.lastname: Xiao
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: S Y
authors.initials: SY
authors.lastname: Shi
authors.forename: Shiyou
authors.initials: S
authors.lastname: Zhou
mesh_terms: D001768:Blister / Q000209:etiology / Q000601:surgery*; D002387:Cataract Extraction / Q000009:adverse effects; D003316:Corneal Diseases / Q000209:etiology / Q000601:surgery*; D003886:Descemet Membrane / Q000601:surgery; D057111:Descemet Stripping Endothelial Keratoplasty; D004728:Endothelium, Corneal; D006085:Graft Survival; D006801:Humans; D015948:Keratoplasty, Penetrating; D011183:Postoperative Complications / Q000601:surgery; D011446:Prospective Studies; D014019:Tissue Donors; D014792:Visual Acuity