title: From single genes to entire genomes: the search for a function of nuclear organization.
abstract: The existence of different domains within the nucleus has been clear from the time, in the late 1920s, that heterochromatin and euchromatin were discovered. The observation that heterochromatin is less transcribed than euchromatin suggested that microscopically identifiable structures might correspond to functionally different domains of the nucleus. Until 15 years ago, studies linking gene expression and subnuclear localization were limited to a few genes. As we discuss in this Review, new genome-wide techniques have now radically changed the way nuclear organization is analyzed. These have provided a much more detailed view of functional nuclear architecture, leading to the emergence of a number of new paradigms of chromatin folding and how this folding evolves during development.
authors.affiliation: Cell Fate and Nuclear Organization, Institute of Cell Biology, University of Bern, 3012 Bern, Switzerland Graduate School for Cellular and Biomedical Sciences, University of Bern, Switzerland.
authors.forename: Ringo
authors.initials: R
authors.lastname: Pueschel
authors.affiliation: Cell Fate and Nuclear Organization, Institute of Cell Biology, University of Bern, 3012 Bern, Switzerland Graduate School for Cellular and Biomedical Sciences, University of Bern, Switzerland.
authors.forename: Francesca
authors.initials: F
authors.lastname: Coraggio
authors.affiliation: Cell Fate and Nuclear Organization, Institute of Cell Biology, University of Bern, 3012 Bern, Switzerland peter.meister@izb.unibe.ch.
authors.forename: Peter
authors.initials: P
authors.lastname: Meister
mesh_terms: D000818:Animals; D019070:Cell Lineage / Q000235:genetics; D002467:Cell Nucleus / Q000235:genetics* / Q000648:ultrastructure; D005796:Genes; D016678:Genome; D006570:Heterochromatin / Q000378:metabolism; D006801:Humans; D008853:Microscopy
chemical_list: D006570:Heterochromatin
keywords: Chromosome conformation capture; Epigenetics; Microscopy; Nuclear organization