title: The hemic response of white-spotted bamboo sharks (Chiloscyllium plagiosum) with inflammatory disease.
abstract: As elasmobranch medicine becomes more commonplace, there continues to be confusion with techniques and evaluation of the shark hemogram and it remains unknown if they are able to mount an inflammatory hemic response. The aims of this study were to compare two total white blood cell (WBC) count techniques, establish a reference interval for captive white-spotted bamboo sharks (Chiloscyllium plagiosum), and determine if elasmobranchs are capable of mounting an inflammatory hemic response. Correlation statistics were performed on hematologic results for healthy female bamboo sharks to assess the use of Natt-Herrick's and phloxine methods. Total WBC counts and differentials were obtained from males with severe traumatic clasper wounds and compared to the healthy females. We elected clasper amputation as the preferred treatment intervention and post-operative hematology was performed one month later. There was poor correlation of leukocyte counts between the two WBC count methods. Hematologic values were established for the females and males pre- and post-operatively. Males with wounds had a marked leukocytosis and heterophilia. Post-operative blood work showed a resolution of total WBC count and a trend toward resolution of the heterophilia. This study provides hematologic values for white-spotted bamboo sharks and confirms that the Natt-Herrick's method is preferred for lymphocytic species. Hematologic differences present in males with clasper wounds suggests that elasmobranchs do mount an inflammatory hemic response. Treatment via clasper amputation proved to be a safe and efficient means for clinical treatment that led to a trend toward resolution of the inflammatory leukogram. Zoo Biol. 35:251-259, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado.
authors.forename: Amy B
authors.initials: AB
authors.lastname: Alexander
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado.
authors.forename: Lily A
authors.initials: LA
authors.lastname: Parkinson
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado.
authors.forename: Krystan R
authors.initials: KR
authors.lastname: Grant
authors.affiliation: Denver Downtown Aquarium, Denver, Colorado.
authors.forename: Eric
authors.initials: E
authors.lastname: Carlson
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado.
authors.forename: Terry W
authors.initials: TW
authors.lastname: Campbell
mesh_terms: D000818:Animals; D000836:Animals, Zoo / Q000502:physiology*; D005260:Female; D005393:Fish Diseases / Q000097:blood / Q000473:pathology / Q000601:surgery; D007249:Inflammation / Q000097:blood / Q000473:pathology / Q000601:surgery / Q000662:veterinary*; D007958:Leukocyte Count / Q000662:veterinary*; D008297:Male; D012016:Reference Values; D015203:Reproducibility of Results; D012754:Sharks / Q000502:physiology*
keywords: cartilaginous fish; clasper; elasmobranchs; hematology; white blood cell count