title: MALDI-TOF mass spectrometry for differentiation between Streptococcus pneumoniae and Streptococcus pseudopneumoniae.
abstract: We compared the Vitek MS and Microflex MALDI-TOF mass spectrometry platform for species differentiation within the Streptococcus mitis group with PCR assays targeted at lytA, Spn9802, and recA as reference standard. The Vitek MS correctly identified 10/11 Streptococcus pneumoniae, 13/13 Streptococcus pseudopneumoniae, and 12/13 S. mitis/oralis. The Microflex correctly identified 9/11 S. pneumoniae, 0/13 S. pseudopneumoniae, and 13/13 S. mitis/oralis. MALDI-TOF is a powerful tool for species determination within the mitis group. Diagnostic accuracy varies depending on platform and database used.
authors.affiliation: Department of Medical Microbiology and Infection Control, VU University Medical Center, Amsterdam, The Netherlands; Department of Medical Microbiology, Onze Lieve Vrouwe Gasthuis, Amsterdam, The Netherlands. Electronic address: j.vanprehn@vumc.nl.
authors.forename: Joffrey
authors.initials: J
authors.lastname: van Prehn
authors.affiliation: Department of Medical Microbiology, Onze Lieve Vrouwe Gasthuis, Amsterdam, The Netherlands.
authors.forename: Suzanne Q
authors.initials: SQ
authors.lastname: van Veen
authors.affiliation: Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Jacqueline J G
authors.initials: JJ
authors.lastname: Schelfaut
authors.affiliation: Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Els
authors.initials: E
authors.lastname: Wessels
mesh_terms: D006801:Humans; D058889:Molecular Typing; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013291:Streptococcus / Q000145:classification* / Q000235:genetics; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000235:genetics
keywords: Diagnostic accuracy; MALDI-TOF; Mass spectrometry; Mitis group; PCR; Streptococcus