title: Interplay between ABA and GA Modulates the Timing of Asymmetric Cell Divisions in the Arabidopsis Root Ground Tissue.
abstract: In multicellular organisms, controlling the timing and extent of asymmetric cell divisions (ACDs) is crucial for correct patterning. During post-embryonic root development in Arabidopsis thaliana, ground tissue (GT) maturation involves an additional ACD of the endodermis, which generates two different tissues: the endodermis (inner) and the middle cortex (outer). It has been reported that the abscisic acid (ABA) and gibberellin (GA) pathways are involved in middle cortex (MC) formation. However, the molecular mechanisms underlying the interaction between ABA and GA during GT maturation remain largely unknown. Through transcriptome analyses, we identified a previously uncharacterized C2H2-type zinc finger gene, whose expression is regulated by GA and ABA, thus named GAZ (GA- AND ABA-RESPONSIVE ZINC FINGER). Seedlings ectopically overexpressing GAZ (GAZ-OX) were sensitive to ABA and GA during MC formation, whereas GAZ-SRDX and RNAi seedlings displayed opposite phenotypes. In addition, our results indicated that GAZ was involved in the transcriptional regulation of ABA and GA homeostasis. In agreement with previous studies that ABA and GA coordinate to control the timing of MC formation, we also confirmed the unique interplay between ABA and GA and identified factors and regulatory networks bridging the two hormone pathways during GT maturation of the Arabidopsis root.
authors.affiliation: Department of Systems Biotechnology, Konkuk University, Seoul 05029, Korea.
authors.forename: Shin Ae
authors.initials: SA
authors.lastname: Lee
authors.affiliation: Department of Systems Biotechnology, Konkuk University, Seoul 05029, Korea.
authors.forename: Sejeong
authors.initials: S
authors.lastname: Jang
authors.affiliation: Department of Systems Biotechnology, Konkuk University, Seoul 05029, Korea.
authors.forename: Eun Kyung
authors.initials: EK
authors.lastname: Yoon
authors.affiliation: Department of Systems Biotechnology, Konkuk University, Seoul 05029, Korea.
authors.forename: Jung-Ok
authors.initials: JO
authors.lastname: Heo
authors.affiliation: Department of Systems Biotechnology, Konkuk University, Seoul 05029, Korea.
authors.forename: Kwang Suk
authors.initials: KS
authors.lastname: Chang
authors.affiliation: Department of Systems Biotechnology, Konkuk University, Seoul 05029, Korea.
authors.forename: Ji Won
authors.initials: JW
authors.lastname: Choi
authors.affiliation: Department of Systems Biotechnology, Konkuk University, Seoul 05029, Korea.
authors.forename: Souvik
authors.initials: S
authors.lastname: Dhar
authors.affiliation: Department of Systems Biotechnology, Konkuk University, Seoul 05029, Korea.
authors.forename: Gyuree
authors.initials: G
authors.lastname: Kim
authors.affiliation: Department of Systems Biotechnology, Konkuk University, Seoul 05029, Korea.
authors.forename: Jeong-Eun
authors.initials: JE
authors.lastname: Choe
authors.affiliation: Department of Molecular Biotechnology, Dong-A University, Busan 49201, Korea.
authors.forename: Jae Bok
authors.initials: JB
authors.lastname: Heo
authors.affiliation: Department of Molecular Biology, Dankook University, Yongin 16890, Korea.
authors.forename: Chian
authors.initials: C
authors.lastname: Kwon
authors.affiliation: Department of Plant and Environmental New Resources, Kyung Hee University, Yongin 17104, Korea.
authors.forename: Jae-Heung
authors.initials: JH
authors.lastname: Ko
authors.affiliation: Department of Systems Biotechnology, Konkuk University, Seoul 05029, Korea.
authors.forename: Yong-Sic
authors.initials: YS
authors.lastname: Hwang
authors.affiliation: Department of Systems Biotechnology, Konkuk University, Seoul 05029, Korea. Electronic address: jlim@konkuk.ac.kr.
authors.forename: Jun
authors.initials: J
authors.lastname: Lim
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D060049:Asymmetric Cell Division / Q000235:genetics / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D005875:Gibberellins / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D005875:Gibberellins; D014157:Transcription Factors; D000040:Abscisic Acid
keywords: ABA; GA; GRAS; ground tissue; root development; transcription factor