title: Influence of silk-silica fusion protein design on silica condensationin vitroand cellular calcification.
abstract: Biomaterial design via genetic engineering can be utilized for the rational functionalization of proteins to promote biomaterial integration and tissue regeneration. Spider silk has been extensively studied for its biocompatibility, biodegradability and extraordinary material properties. As a protein-based biomaterial, recombinant DNA derived derivatives of spider silks have been modified with biomineralization domains which lead to silica deposition and potentially accelerated bone regeneration. However, the influence of the location of the R5 (SSKKSGSYSGSKGSKRRIL) silicifying domain fused with the spider silk protein sequence on the biosilicification process remains to be determined. Here we designed two silk-R5 fusion proteins that differed in the location of the R5 peptide, C-vs. N-terminus, where the spider silk domain consisted of a 15mer repeat of a 33 amino acid consensus sequence of the major ampullate dragline Spidroin 1 fromNephila clavipes(SGRGGLGGQG AGAAAAAGGA GQGGYGGLGSQGT). The chemical, physical and silica deposition properties of these recombinant proteins were assessed and compared to a silk 15mer control without the R5 present. The location of the R5 peptide did not have a significant effect on wettability and surface energies, while the C-terminal location of the R5 promoted more controlled silica precipitation, suggesting differences in protein folding and possibly different access to charged amino acids that drive the silicification process. Further, cell compatibilityin vitro, as well as the ability to promote human bone marrow derived mesenchymal stem cell (hMSC) differentiation were demonstrated for both variants of the fusion proteins.
authors.affiliation: Biomolecular and Materials Interface Research Group, Interdisciplinary Biomedical Research Centre, School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham, UK NG11 8NS.
authors.forename: Robyn
authors.initials: R
authors.lastname: Plowright
authors.affiliation: Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, Massachusetts, 02155, United States.
authors.forename: Nina
authors.initials: N
authors.lastname: Dinjaski
authors.affiliation: Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, Massachusetts, 02155, United States.
authors.forename: Shun
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Biomolecular and Materials Interface Research Group, Interdisciplinary Biomedical Research Centre, School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham, UK NG11 8NS.
authors.forename: David J
authors.initials: DJ
authors.lastname: Belton
authors.affiliation: Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, Massachusetts, 02155, United States.
authors.forename: David L
authors.initials: DL
authors.lastname: Kaplan
authors.affiliation: Biomolecular and Materials Interface Research Group, Interdisciplinary Biomedical Research Centre, School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham, UK NG11 8NS.
authors.forename: Carole C
authors.initials: CC
authors.lastname: Perry
keywords: biomaterials; biomineralization; fusion proteins; silaffin; spider silk