title: Expression Depression of CD300LG-γ in Human Pulmonary Carcinoma.
abstract: The expression of CD300LG-γ at mRNA and protein level was detected by semiquantitative reverse transcription-polymerase chain reaction (PCR), quantitative real-time PCR, and western blot, and compared by t-test analysis. The results of semiquantitative reverse transcription-PCR showed that the mRNA expression of CD300LG-γ was significantly lower in pulmonary carcinoma tissues, compared to tumor-adjacent tissues. The results of quantitative real-time PCR confirmed this finding. The mRNA level of CD300LG-γ in pulmonary carcinoma tissues compared with tumor-adjacent tissues was 0.436-fold in a median. The results of western blot indicated that CD300LG-γ protein was expressed in both pulmonary carcinoma and tumor-adjacent tissues, and the expression level was significantly lower in pulmonary carcinoma tissues compared with tumor-adjacent tissues. Both mRNA and protein levels of CD300LG-γ in pulmonary carcinoma tissues were significantly lower than that in tumor-adjacent tissues, which might lead to inhibition of killing function of immunocytes, resulting in immune escape of lung cancer cells.
authors.affiliation: 1 Department of Pediatrics, West China Second University Hospital, Sichuan University , Chengdu, China .
authors.forename: Songhui
authors.initials: S
authors.lastname: Zhai
authors.affiliation: 2 Department of Immunology, College of Preclinical and Forensic Medicine, Sichuan University , Chengdu, China .
authors.forename: He
authors.initials: H
authors.lastname: Xu
authors.affiliation: 2 Department of Immunology, College of Preclinical and Forensic Medicine, Sichuan University , Chengdu, China .
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Jiang
authors.affiliation: 3 Insitute of Forensic Science, Chongqing Public Security Bureau , Chongqing, China .
authors.forename: Sheng
authors.initials: S
authors.lastname: Sun
authors.affiliation: 4 The Reproductive Centre, Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University , Chengdu, China .
authors.forename: Lang
authors.initials: L
authors.lastname: Qin
authors.affiliation: 2 Department of Immunology, College of Preclinical and Forensic Medicine, Sichuan University , Chengdu, China .
authors.forename: Dapeng
authors.initials: D
authors.lastname: Wei
authors.affiliation: 2 Department of Immunology, College of Preclinical and Forensic Medicine, Sichuan University , Chengdu, China .
authors.forename: Lijuan
authors.initials: L
authors.lastname: Hu
mesh_terms: D015703:Antigens, CD / Q000096:biosynthesis / Q000276:immunology*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000276:immunology*
chemical_list: D015703:Antigens, CD; C584286:CD300LG protein, human; D012333:RNA, Messenger; D011971:Receptors, Immunologic