title: Microdialysis combined with liquid chromatography-tandem mass spectrometry for the determination of nimodipine in the guinea pig hippocampus.
abstract: Nimodipine is a dihydropyridine calcium-channel blocker that has been recently shown to be effective on the function of central nervous system. It has been reported that treatment against deficits of learning and memory in animals and human by maintain the calcium homeostasis in hippocampus with nimodipine may be promising therapeutic strategies. A rapid and sensitive liquid chromatography-tandem mass spectrometric method was developed to determination the nimodipine in hippocampus using microdialysis technique. The separation was accomplished on an Agilent Zorbax SB-C18 column (100mm×2.1mm ID, 3.5μm) with the mobile phase composed of methanol-water (80:20, v/v) containing 0.2% formic acid at a flow rate of 0.2ml/min. Multiple reaction monitoring of the precursor-product ion transitions 419→343 for nimodipine and 361→315 nitrendipine (IS) was used for quantitation. The method was sensitive with a lower limit of quantitation (LLOQ) of 0.2ng/ml for nimodipine, with good linearity in the range of 0.2-20ng/ml. All the validation data, such as accuracy, precision, intra- and inter-day repeatability and stability were within the required limits. The method was successfully applied to p harmacokinetic study of the nimodipine in the guinea pig hippocampus.
authors.affiliation: State Key Laboratory of NBC Protection for Civilian, Beijing 102205, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.affiliation: State Key Laboratory of NBC Protection for Civilian, Beijing 102205, PR China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Lu
authors.affiliation: State Key Laboratory of NBC Protection for Civilian, Beijing 102205, PR China. Electronic address: liliqinpla@163.com.
authors.forename: Liqin
authors.initials: L
authors.lastname: Li
authors.affiliation: State Key Laboratory of NBC Protection for Civilian, Beijing 102205, PR China.
authors.forename: Ruihua
authors.initials: R
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of NBC Protection for Civilian, Beijing 102205, PR China.
authors.forename: Tong
authors.initials: T
authors.lastname: Shi
authors.affiliation: Department of Pharmacy, Navy General Hospital, Beijing 100048, PR China. Electronic address: judy_lishu@163.com.
authors.forename: Shu
authors.initials: S
authors.lastname: Li
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D006168:Guinea Pigs; D006624:Hippocampus / Q000378:metabolism*; D017551:Microdialysis; D009553:Nimodipine / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009553:Nimodipine
keywords: Hippocampus; LC–MS/MS; Microdialysis; Nimodipine; Pharmacokinetics