title: Light scattering microscopy measurements of single nuclei compared with GPU-accelerated FDTD simulations.
abstract: Single cell nuclei were investigated using two-dimensional angularly and spectrally resolved scattering microscopy. We show that even for a qualitative comparison of experimental and theoretical data, the standard Mie model of a homogeneous sphere proves to be insufficient. Hence, an accelerated finite-difference time-domain method using a graphics processor unit and domain decomposition was implemented to analyze the experimental scattering patterns. The measured cell nuclei were modeled as single spheres with randomly distributed spherical inclusions of different size and refractive index representing the nucleoli and clumps of chromatin. Taking into account the nuclear heterogeneity of a large number of inclusions yields a qualitative agreement between experimental and theoretical spectra and illustrates the impact of the nuclear micro- and nanostructure on the scattering patterns.
authors.affiliation: Institut für Lasertechnologien in der Medizin und Meßtechnik (ILM), Helmholtzstr. 12, 89081 Ulm, Germany.
authors.forename: Julian
authors.initials: J
authors.lastname: Stark
authors.forename: Thomas
authors.initials: T
authors.lastname: Rothe
authors.forename: Steffen
authors.initials: S
authors.lastname: Kieß
authors.forename: Sven
authors.initials: S
authors.lastname: Simon
authors.forename: Alwin
authors.initials: A
authors.lastname: Kienle
mesh_terms: D016475:3T3 Cells; D000465:Algorithms; D000818:Animals; D016466:CHO Cells; D002467:Cell Nucleus / Q000648:ultrastructure*; D006224:Cricetinae; D003412:Cricetulus; D008027:Light; D051379:Mice; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods; D012542:Scattering, Radiation; D059010:Single-Cell Analysis / Q000295:instrumentation / Q000379:methods