title: Preinduced intestinal HSP70 improves visceral hypersensitivity and abnormal intestinal motility in PI-IBS mouse model.
abstract: OBJECTIVE
  To investigate the impact of the preinduced intestinal heat shock protein 70 (HSP70) on the visceral hypersensitivity and abnormal intestinal motility in a post-infectious irritable bowel syndrome (PI-IBS) mouse model.
  
  
  METHODS
  Eighty-four female C57BL/6 mice were randomly assigned to four groups: control group (n = 21) and induction + PI-IBS group (n = 21), PI-IBS group (n = 21) and induction group (n = 21). The mice in PI-IBS group were infected in vivo with Trichinella spiralis by oral administration. The visceral hypersensitivity and intestinal motility were evaluated respectively with abdominal withdrawal reflex and colon transportation test. The intestinal HSP70 protein and mRNA level was measured by Western blot and real-time PCR. Meanwhile, the intestinal proinflammatory cytokines IL-10 and TNF-α level was detected by ELISA.
  
  
  RESULTS
  Compared with their counterparts in PI-IBS group, the animals in the Induction + PI-IBS group show significantly increased intestinal level of HSP70 and obviously ameliorative clinical figures, including abdominal withdrawal reflex score, intestine transportation time and Bristol scores (P < 0.05). Meanwhile, the intestinal post-inflammatory cytokines remarkably changed, including increased IL-10 level and decreased TNF-α level (P < 0.05).
  
  
  CONCLUSIONS
  Intestinal HSP70 may play a potential protective role through improving the imbalance between the intestinal post-inflammatory and anti-inflammatory cytokines in PI-IBS.
authors.affiliation: Department of Gastroenterology, Hainan Provincial General Hospital, Haikou 570311, Hainan Province, China. Electronic address: lancheng71@163.com.
authors.forename: Cheng
authors.initials: C
authors.lastname: Lan
authors.affiliation: Department of Gastroenterology, Hainan Provincial General Hospital, Haikou 570311, Hainan Province, China.
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: Sun
authors.affiliation: Department of Gastroenterology, Hainan Provincial General Hospital, Haikou 570311, Hainan Province, China.
authors.forename: Xu-Chun
authors.initials: XC
authors.lastname: Zhou
authors.affiliation: Department of Gastroenterology, 1st Affiliated Hospital of Chongqing Medical University, Chongqing 400016, Chongqing, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Gastroenterology, Hainan Provincial General Hospital, Haikou 570311, Hainan Province, China.
authors.forename: Bai-Li
authors.initials: BL
authors.lastname: Huang
authors.affiliation: Department of Gastroenterology, Hainan Provincial General Hospital, Haikou 570311, Hainan Province, China.
authors.forename: Tao-Zhi
authors.initials: TZ
authors.lastname: Deng
authors.affiliation: Department of Gastroenterology, Hainan Provincial General Hospital, Haikou 570311, Hainan Province, China.
authors.forename: Zhou-Tao
authors.initials: ZT
authors.lastname: He
authors.affiliation: Department of Gastroenterology, Hainan Provincial General Hospital, Haikou 570311, Hainan Province, China.
authors.forename: Xiang-Yang
authors.initials: XY
authors.lastname: Han
keywords: Heat shock protein 70; Intestinal motility; Post-infectious irritable bowel syndrome; Proinflammatory cytokine; Visceral hypersensitivity