title: Antioxidant capacity and phenolic compounds of Lonicerae macranthoides by HPLC-DAD-QTOF-MS/MS.
abstract: Lonicerae macranthoides with strong antioxidant activity is commonly used in traditional Chinese medicine and folk tea/beverage. However, detailed information about its antioxidant activity and bioactive compounds is limited. Then at first, we comparatively evaluated total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activities of water extract, petroleum ether, ethyl acetate and n-butanol fractions of L. macranthoides. Ethyl acetate fraction exhibited the highest level of TPC (207.38 mg GAE/g DW), TFC (53.06 mg RE/g DW) and the best DPPH scavenge activity and reducing power. n-Butanol fraction showed the best ABTS(+) and O2(-) scavenging activities. Interestingly, water extract, ethyl acetate and n-butanol fractions showed stronger antioxidant activities than positive control, butylated hydroxytoluene (BHT). After that, thirty-one antioxidant phenolic compounds, including twenty-two phenolic acids and nine flavonoids, were screened by DPPH-HPLC experiment and then identified using HPLC-DAD-QTOF-MS/MS. It is noted that twenty-one compounds (1, 3-4, 6-17, 19, 23, 26, 28-29, and 31), as far as was known, were discovered from L. macranthoide for the first time, and eleven of them (3-4, 10-17, and 23) were reported in Lonicera species for the first time. Results indicated that L. macranthoides could serve as promising source of rich antioxidants in foods, beverages and medicines for health promotion.
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Hu
authors.affiliation: Research Institute of Chinese Medicine, Hunan Academy of Chinese Medicine, Changsha 410013, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China. Electronic address: shuyshi@gmail.com.
authors.forename: Shuyun
authors.initials: S
authors.lastname: Shi
authors.affiliation: Research Institute of Chinese Medicine, Hunan Academy of Chinese Medicine, Changsha 410013, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Cai
authors.affiliation: Research Institute of Chinese Medicine, Hunan Academy of Chinese Medicine, Changsha 410013, PR China.
authors.forename: Xuejuan
authors.initials: X
authors.lastname: Liang
authors.affiliation: Research Institute of Chinese Medicine, Hunan Academy of Chinese Medicine, Changsha 410013, PR China. Electronic address: zhangshuihan0220@126.com.
authors.forename: Shuihan
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031239:Lonicera / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000975:Antioxidants; D010636:Phenols
keywords: Antioxidant activity; Flavonoid; HPLC–DAD–QTOF-MS/MS; Lonicerae macranthoides; Phenolic acid