title: Potential application of silver nanoparticles to control the infectivity of Rift Valley fever virus in vitro and in vivo.
abstract: In this work we have tested the potential antiviral activity of silver nanoparticles formulated as Argovit™ against Rift Valley fever virus (RVFV). The antiviral activity of Argovit was tested on Vero cell cultures and in type-I interferon receptor deficient mice (IFNAR (-/-) mice) by two different approaches: (i) different dilutions of Argovit were added to previously infected cells or administrated to animals infected with a lethal dose of virus; (ii) virus was pre-incubated with different dilutions of Argovit before inoculation in mice or cells. Though the ability of silver nanoparticles to control an ongoing RVFV infection in the conditions tested was limited, the incubation of virus with Argovit before the infection led to a reduction of the infectivity titers both in vitro and in vivo. These results reveal the potential application of silver nanoparticles to control the infectivity of RVFV, which is an important zoonotic pathogen.
authors.affiliation: Centro de Investigación en Sanidad Animal - INIA (National Research Institute for Agricultural and Food Technology), Valdeolmos, Madrid, Spain. Electronic address: borrego@inia.es.
authors.forename: Belén
authors.initials: B
authors.lastname: Borrego
authors.affiliation: Centro de Investigación en Sanidad Animal - INIA (National Research Institute for Agricultural and Food Technology), Valdeolmos, Madrid, Spain.
authors.forename: Gema
authors.initials: G
authors.lastname: Lorenzo
authors.affiliation: CONACYT Research Fellow - Centro de Nanociencias y Nanotecnología (CNyN), Universidad Nacional Autónoma de México (UNAM), Ensenada, BC, Mexico. Electronic address: jmota@cnyn.unam.mx.
authors.forename: Josué D
authors.initials: JD
authors.lastname: Mota-Morales
authors.affiliation: Facultad de Medicina y Psicología-Universidad Autónoma Baja California, Tijuana, Mexico.
authors.forename: Horacio
authors.initials: H
authors.lastname: Almanza-Reyes
authors.affiliation: Centro de Investigación en Sanidad Animal - INIA (National Research Institute for Agricultural and Food Technology), Valdeolmos, Madrid, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Mateos
authors.affiliation: Centro de Investigación en Sanidad Animal - INIA (National Research Institute for Agricultural and Food Technology), Valdeolmos, Madrid, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: López-Gil
authors.affiliation: Centro de Investigación en Sanidad Animal - INIA (National Research Institute for Agricultural and Food Technology), Valdeolmos, Madrid, Spain.
authors.forename: Nuria
authors.initials: N
authors.lastname: de la Losa
authors.affiliation: Scientific-Production Centre "Vektor Vita", Novosibirsk, Russia.
authors.forename: Vasily A
authors.initials: VA
authors.lastname: Burmistrov
authors.affiliation: Tomsk Polytechnic University, Tomsk, Russia.
authors.forename: Alexey N
authors.initials: AN
authors.lastname: Pestryakov
authors.affiliation: Centro de Investigación en Sanidad Animal - INIA (National Research Institute for Agricultural and Food Technology), Valdeolmos, Madrid, Spain.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Brun
authors.affiliation: Centro de Nanociencias y Nanotecnología (CNyN), Universidad Nacional Autónoma de México (UNAM), Ensenada, Mexico.
authors.forename: Nina
authors.initials: N
authors.lastname: Bogdanchikova
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D051379:Mice; D053758:Nanoparticles / Q000627:therapeutic use*; D012295:Rift Valley Fever / Q000517:prevention & control; D012296:Rift Valley fever virus / Q000187:drug effects* / Q000472:pathogenicity; D012834:Silver / Q000627:therapeutic use*
chemical_list: D000998:Antiviral Agents; D012834:Silver
keywords: Antivirals; In vivo infection; Rift Valley fever virus; Silver nanoparticles