title: Ammonia-LCFA synergetic co-inhibition effect in manure-based continuous biomethanation process.
abstract: In the current study it has been hypothesized that, when organic loading of an anaerobic reactor is increased, the additional cell biomass biosynthesis would capture more ammonia nitrogen and thereby reduce the ammonia toxicity. Therefore, the alleviation of the toxicity of high ammonia levels using lipids (glycerol trioleate-GTO) or carbohydrates (glucose-GLU) as co-substrates in manure-based thermophilic continuous stirred-tank reactors (R(GTO) and R(GLU), respectively) was tested. At 5gNH4(+)-NL(-1), relative methane production of R(GTO) and R(GLU), was 10.5% and 41% compared to the expected uninhibited production, respectively. At the same time control reactor (R(CTL)), only fed with manure, reached 32.7% compared to the uninhibited basis production. Therefore, it seems that using lipids to counteract the ammonia effect in CSTR reactors creates an "ammonia-LCFA (long chain fatty acids) synergetic co-inhibition" effect. Moreover, co-digestion with glucose in R(GLU) was more robust to ammonia toxicity compared to R(CTL).
authors.affiliation: Department of Environmental Engineering, Technical University of Denmark, Building 113, DK-2800 Kgs. Lyngby, Denmark.
authors.forename: Han
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Environmental Engineering, Technical University of Denmark, Building 113, DK-2800 Kgs. Lyngby, Denmark. Electronic address: ioanf@env.dtu.dk.
authors.forename: Ioannis A
authors.initials: IA
authors.lastname: Fotidis
authors.affiliation: Department of Environmental Engineering, Technical University of Denmark, Building 113, DK-2800 Kgs. Lyngby, Denmark.
authors.forename: Irini
authors.initials: I
authors.lastname: Angelidaki
mesh_terms: D000641:Ammonia / Q000032:analysis*; D000693:Anaerobiosis; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D005227:Fatty Acids / Q000032:analysis*; D005232:Fatty Acids, Volatile / Q000032:analysis; D005990:Glycerol / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008372:Manure / Q000032:analysis*; D008697:Methane / Q000096:biosynthesis*; D009586:Nitrogen Fixation
chemical_list: D005227:Fatty Acids; D005232:Fatty Acids, Volatile; D008372:Manure; D000641:Ammonia; D008697:Methane; D005990:Glycerol
keywords: Ammonia toxicity; Anaerobic digestion; Co-digestion; Hydrogenotrophic methanogens; Lipids