title: Structuring Au nanoparticles on two-dimensional MoS2nanosheets for electrochemical glucose biosensors.
abstract: Two-dimensional (2D) bioelectronics is an emerging field of research which fuses the advantages of 2D nanomaterials with those of nanobiotechnology. Due to the various physical and chemical properties present in layered counterparts of 2D materials, including high charge density, large surface area, remarkable electron mobility, ready electron transport, sizeable band gaps and ease of hybridisation, they are set to become a versatile tool to fabricate sensitive and selective novel biodevices, which might offer an unique advantages to tackle key energy, medical and environmental issues. Current 2D bioelectronics research is focused on the design of simple-to-use and cheaper biodevices, while improving their selectivity, sensitivity and stability. However, current designs generally suffer from a lack of efficiency, relatively low sensitivity, slow electron transfer kinetics, high background charging current and low current density arising from poor mass transport. Here, we report a nanoparticle-structured MoS2nanosheet as an ideal semiconductor interface, which is able to form a homogenous layer on the electrode surface for the assembly of gold nanoparticles. This not only enhances electrocatalytic reactions, but also provides excellent electrochemical properties such as high faradic-to-capacitive current ratios, high current density and electron mobility, and faster mass transport, due to the dominance of radial diffusion. The MoS2/Au NPs/GOx bioelectrode exhibits a linear response to glucose from 0.25 to 13.2mM, with a detection limit of 0.042µM (S/N=3) and sensitivity of 13.80µA/µM/cm2.
authors.affiliation: Biosensors and Bioelectronics Centre, IFM, Linköping University, 581 83 Linköping, Sweden. Electronic address: onur.parlak@liu.se.
authors.forename: Onur
authors.initials: O
authors.lastname: Parlak
authors.affiliation: Biosensors and Bioelectronics Centre, IFM, Linköping University, 581 83 Linköping, Sweden.
authors.forename: Anıl
authors.initials: A
authors.lastname: İncel
authors.affiliation: Biosensors and Bioelectronics Centre, IFM, Linköping University, 581 83 Linköping, Sweden; Biochemistry Division, Department of Chemistry, Hacettepe University, Ankara, Turkey.
authors.forename: Lokman
authors.initials: L
authors.lastname: Uzun
authors.affiliation: Biosensors and Bioelectronics Centre, IFM, Linköping University, 581 83 Linköping, Sweden.
authors.forename: Anthony P F
authors.initials: APF
authors.lastname: Turner
authors.affiliation: Biosensors and Bioelectronics Centre, IFM, Linköping University, 581 83 Linköping, Sweden.
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Tiwari
mesh_terms: D001234:Aspergillus niger / Q000737:chemistry / Q000201:enzymology; D015374:Biosensing Techniques / Q000295:instrumentation; D004220:Disulfides / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D008958:Models, Molecular; D008982:Molybdenum / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D012666:Semiconductors
chemical_list: D004220:Disulfides; D004800:Enzymes, Immobilized; D006046:Gold; D008982:Molybdenum; D005949:Glucose Oxidase; D005947:Glucose; C082964:molybdenum disulfide
keywords: 2D materials; Biosensor; MoS(2) nanosheets; Nano-biointerface; Self-assembly