title: Sensitive liquid chromatography/tandem mass spectrometry method for the simultaneous determination of nine local anesthetic drugs.
abstract: A high-performance liquid chromatography-tandem mass spectrometry (LC/MS/MS) with electrospray ionization (ESI) procedure for the simultaneous determination of nine local anesthetic drugs (procaine, mepivacaine, lidocaine, ropivacaine, oxybuprocaine, tetracaine, bupivacaine, T-caine and dibucaine) in human serum is described. The chromatographic separation was performed on a Mightysil-RP-18 GP II column (2.0mm×150mm, particle size 5μm). The mobile phase consisted of 10mM acetic ammonium buffer (pH 5.4) and acetonitrile and was delivered at a flow rate of 0.20mL/min. The triple quadrupole mass spectrometer was operated in positive ion mode, and multiple reaction monitoring was used for drug quantification. Solid-phase extraction of the nine local anesthetic drugs added to the human serum was performed with an Oasis(®) HLB extraction cartridges column. The method was linear for the investigated drugs over the concentration range of 10-100ng/mL. The recoveries of these drugs were in the range of 81.4-144%. The standard deviation (SD) values for all analytes were <0.10 for both intraday and interday accuracy and precision. The selectivity, accuracy and precision of this method are satisfactory for clinical and forensic applications. The sensitive and selective method offers the opportunity for the simultaneous screening and quantification, for clinical and forensic purposes, of almost all local anesthetics available in Japan.
authors.affiliation: Department of Pathophysiology, Yokohama University of Pharmacy, 601 Matanocho, Totsuka-ku, Yokohama, Kanagawa 245-0066, Japan. Electronic address: k.tonooka@ym.hamayaku.ac.jp.
authors.forename: Keiko
authors.initials: K
authors.lastname: Tonooka
authors.affiliation: Department of Pathophysiology, Yokohama University of Pharmacy, 601 Matanocho, Totsuka-ku, Yokohama, Kanagawa 245-0066, Japan.
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Naruki
authors.affiliation: Department of Pathophysiology, Yokohama University of Pharmacy, 601 Matanocho, Totsuka-ku, Yokohama, Kanagawa 245-0066, Japan.
authors.forename: Kou
authors.initials: K
authors.lastname: Honma
authors.affiliation: Department of Pathophysiology, Yokohama University of Pharmacy, 601 Matanocho, Totsuka-ku, Yokohama, Kanagawa 245-0066, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Agei
authors.affiliation: Department of Pathophysiology, Yokohama University of Pharmacy, 601 Matanocho, Totsuka-ku, Yokohama, Kanagawa 245-0066, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Okutsu
authors.affiliation: Department of Pathophysiology, Yokohama University of Pharmacy, 601 Matanocho, Totsuka-ku, Yokohama, Kanagawa 245-0066, Japan.
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Hosono
authors.affiliation: Foundation for Promotion of Material Science and Technology of Japan, 1-18-6 Kitami, Setagaya-ku, Tokyo 157-0067, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kunisue
authors.affiliation: Department of Legal Medicine, School of Medicine, Toho University, 5-21-16 Oomorinishi, Ota-ku, Tokyo 143-8540.
authors.forename: Masaru
authors.initials: M
authors.lastname: Terada
authors.affiliation: Department of Pathophysiology, Yokohama University of Pharmacy, 601 Matanocho, Totsuka-ku, Yokohama, Kanagawa 245-0066, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Tomobe
authors.affiliation: Department of Pathophysiology, Yokohama University of Pharmacy, 601 Matanocho, Totsuka-ku, Yokohama, Kanagawa 245-0066, Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Shinozuka
mesh_terms: D000779:Anesthetics, Local / Q000097:blood*; D002853:Chromatography, Liquid; D053593:Forensic Toxicology; D006801:Humans; D011237:Predictive Value of Tests; D015813:Substance Abuse Detection / Q000379:methods; D019966:Substance-Related Disorders / Q000097:blood / Q000175:diagnosis*; D053719:Tandem Mass Spectrometry
chemical_list: D000779:Anesthetics, Local
keywords: LC/MS/MS, Local anesthetic, Analysis, Solid phase extraction