title: The Effects of Granulocyte-Colony Stimulating Factor on Regeneration in Nerve Crush Injuries in Rats.
abstract: Granulocyte-colony stimulating factor (G-CSF) is widely known to have a neuroprotective effect, but its effects on function and morphology in mechanical nerve injury are not well understood. The aim of this study was to confirm the time course of the functional changes and morphological effects of G-CSF in a rat model of nerve crush injury. Twelve-eight rats were divided into three group: sham-operated control group, G-CSF-treated group, and saline treated group. 2 weeks after the nerve crush injury, G-CSF was injected for 5 days. After 4 weeks, functional tests such as motor nerve conduction velocity (MNCV), mechanical and cold allodynia tests, and morphological studies were performed. G-CSF-treated rats had significantly improved nerve function including MNCV and mechanical and cold allodynia. In addition, G-CSF-treated rats had significantly higher the density of myelinated fibers than saline-treated rats. In conclusion, we found that 100 μg/kg administration of G-CSF promoted long-term functional recovery in a rat model of nerve crush injury.
authors.affiliation: Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, South Korea.
authors.forename: Yi-Sun
authors.initials: YS
authors.lastname: Song
authors.affiliation: Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, South Korea.
authors.forename: Jun-Ho
authors.initials: JH
authors.lastname: Joe
authors.affiliation: Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, South Korea.
authors.forename: Hyun-Woo
authors.initials: HW
authors.lastname: Joo
authors.affiliation: Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, South Korea.
authors.forename: In-Hwa
authors.initials: IH
authors.lastname: Park
authors.affiliation: Cardiology Division, Department of Internal Medicine, Hanyang University College of Medicine, Seoul, South Korea.
authors.forename: Guang-Yin
authors.initials: GY
authors.lastname: Shen
authors.affiliation: Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, South Korea.
authors.forename: Ki-Jun
authors.initials: KJ
authors.lastname: Kim
authors.affiliation: Department of Cardiology, Sungae Hospital, Seoul, South Korea.
authors.forename: Yonggu
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Cardiology Division, Department of Internal Medicine, Hanyang University College of Medicine, Seoul, South Korea.
authors.forename: Jeong Hun
authors.initials: JH
authors.lastname: Shin
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Hanyang University College of Medicine, Seoul, South Korea.
authors.forename: Hyuck
authors.initials: H
authors.lastname: Kim
authors.affiliation: Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, South Korea. kskim@hanyang.ac.kr.|Cardiology Division, Department of Internal Medicine, Hanyang University College of Medicine, Seoul, South Korea. kskim@hanyang.ac.kr.
authors.forename: Kyung-Soo
authors.initials: KS
authors.lastname: Kim
mesh_terms: D000818:Animals; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D009409:Nerve Crush / Q000379:methods; D009416:Nerve Regeneration / Q000187:drug effects / Q000502:physiology*; D009431:Neural Conduction / Q000187:drug effects / Q000502:physiology; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020426:Sciatic Neuropathy / Q000188:drug therapy* / Q000473:pathology; D016896:Treatment Outcome
chemical_list: D018696:Neuroprotective Agents; D016179:Granulocyte Colony-Stimulating Factor
keywords: Allodynia; Granulocyte-colony stimulating factor; Mechanical nerve injury; Nerve crush injury; Nerve function