title: First insight into the molecular epidemiology of Mycobacterium tuberculosis in Santa Catarina, southern Brazil.
abstract: Molecular epidemiology of Mycobacterium tuberculosis is useful for understanding disease transmission dynamics, and to establish strategic measures for TB control and prevention. The aim of this study was to analyze clinical, epidemiological and molecular characteristics of MTBC clinical isolates from Santa Catarina state, southern Brazil. During one-year period, 406 clinical isolates of MTBC were collected from Central Laboratory of Public Health and typed by spoligotyping. Demographic and clinical data were collected from the Brazilian National Mandatory Disease Reporting System. The majority of cases occurred in highest population densities regions and about 50% had some condition associated with TB. Among all isolates, 5.7% were MDR, which showed association with drug addiction. LAM was the most predominant lineage with 47.5%, followed by the T superfamily with 25.9% and Haarlem with 12.3%. The MST showed two major groups: the first was formed mainly by the LAM lineage and the second was mainly formed by the T and Haarlem lineages. Others lineages were distributed in peripheral positions. This study provides the first insight into the population structure of M. tuberculosis in SC State. Spoligotyping and other genotyping analyses are important to establish strategic measures for TB control and prevention.
authors.affiliation: Universidade Federal de Santa Catarina - UFSC, Campus Universitário, s/n. Florianópolis, Santa Catarina, Brazil. Electronic address: nogueira.chris@gmail.com.
authors.forename: Christiane Lourenço
authors.initials: CL
authors.lastname: Nogueira
authors.affiliation: Universidade Federal de Santa Catarina - UFSC, Campus Universitário, s/n. Florianópolis, Santa Catarina, Brazil. Electronic address: rodrigo.prim@posgrad.ufsc.br.
authors.forename: Rodrigo Ivan
authors.initials: RI
authors.lastname: Prim
authors.affiliation: Universidade Federal de Santa Catarina - UFSC, Campus Universitário, s/n. Florianópolis, Santa Catarina, Brazil. Electronic address: simonesenna@hotmail.com.
authors.forename: Simone Gonçalves
authors.initials: SG
authors.lastname: Senna
authors.affiliation: Laboratório Central Do Estado de Santa Catarina - LACEN/SC, Florianópolis, Santa Catarina, Brazil. Electronic address: darcitarovaris@gmail.com.
authors.forename: Darcita Büerger
authors.initials: DB
authors.lastname: Rovaris
authors.affiliation: Universidade Federal de Santa Catarina - UFSC, Campus Universitário, s/n. Florianópolis, Santa Catarina, Brazil. Electronic address: rosemaurici@gmail.com.
authors.forename: Rosemeri
authors.initials: R
authors.lastname: Maurici
authors.affiliation: Fundação Estadual de Produção e Pesquisa em Saúde Do Rio Grande Do Sul - FEEPS/RS, Porto Alegre, Rio Grande Do Sul, Brazil. Electronic address: mrossett@terra.com.br.
authors.forename: Maria Lúcia
authors.initials: ML
authors.lastname: Rossetti
authors.affiliation: WHO Supranational TB Reference Laboratory, Institut Pasteur de la Guadeloupe, Abymes, Guadeloupe, France. Electronic address: david.couvin@googlemail.com.
authors.forename: David
authors.initials: D
authors.lastname: Couvin
authors.affiliation: WHO Supranational TB Reference Laboratory, Institut Pasteur de la Guadeloupe, Abymes, Guadeloupe, France. Electronic address: nrastogi@pasteur-guadeloupe.fr.
authors.forename: Nalin
authors.initials: N
authors.lastname: Rastogi
authors.affiliation: Universidade Federal de Santa Catarina - UFSC, Campus Universitário, s/n. Florianópolis, Santa Catarina, Brazil. Electronic address: m.l.bazzo@ufsc.br.
authors.forename: Maria Luiza
authors.initials: ML
authors.lastname: Bazzo
mesh_terms: D000437:Alcoholism; D001431:Bacteriological Techniques; D001938:Brazil / Q000453:epidemiology; D060085:Coinfection; D015897:Comorbidity; D005260:Female; D005838:Genotype; D015658:HIV Infections / Q000453:epidemiology; D006801:Humans; D008297:Male; D025202:Molecular Diagnostic Techniques; D017720:Molecular Epidemiology; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000472:pathogenicity; D010641:Phenotype; D012307:Risk Factors; D019966:Substance-Related Disorders / Q000453:epidemiology; D014376:Tuberculosis / Q000453:epidemiology* / Q000382:microbiology* / Q000517:prevention & control / Q000635:transmission
keywords: Molecular epidemiology; Santa Catarina; Spoligotyping; Tuberculosis