title: Rapid Assembly of Heterogeneous 3D Cell Microenvironments in a Microgel Array.
abstract: Heterogeneous 3D cell microenvironment arrays are rapidly assembled by combining surface-wettability-guided assembly and microdroplet-array-based operations. This approach enables precise control over individual shapes, sizes, chemical concentrations, cell density, and 3D spatial distribution of multiple components. This technique provides a cost-effective solution to meet the increasing demand of stem cell research, tissue engineering, and drug screening.
authors.affiliation: Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China.|Department of Applied Physics, John A. Paulson School of Engineering and Applied Sciences, Harvard University Cambridge, MA, 02138, USA.
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Radiology, School of Medicine, Stanford University, Palo Alto, CA, 94304, USA.
authors.forename: Pu
authors.initials: P
authors.lastname: Chen
authors.affiliation: Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China.
authors.forename: Yachao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China.
authors.forename: Shuangqian
authors.initials: S
authors.lastname: Yan
authors.affiliation: Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Feng
authors.affiliation: Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Du
authors.affiliation: Department of Applied Physics, John A. Paulson School of Engineering and Applied Sciences, Harvard University Cambridge, MA, 02138, USA.
authors.forename: Stephan A
authors.initials: SA
authors.lastname: Koehler
authors.affiliation: Department of Radiology, School of Medicine, Stanford University, Palo Alto, CA, 94304, USA.
authors.forename: Utkan
authors.initials: U
authors.lastname: Demirci
authors.affiliation: Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China.
authors.forename: Bi-Feng
authors.initials: BF
authors.lastname: Liu
mesh_terms: D060833:Cellular Microenvironment; D023822:Tissue Engineering
keywords: cell microenvironment; heterogeneous microenvironment arrays; microgel assembly; tissue engineering