title: Molecular Mechanism of Pin1-Tau Recognition and Catalysis.
abstract: Human peptidyl-prolyl isomerase (PPIase) Pin1 plays key roles in developmental processes, cell proliferation, and neuronal function. Extensive phosphorylation of the microtubule binding protein tau has been implicated in neurodegeneration and Alzheimer's disease. For the past 15years, these two players have been the focus of an enormous research effort to unravel the biological relevance of their interplay in health and disease, resulting in a series of proposed molecular mechanism of how Pin1 catalysis of tau results in biological phenotypes. Our results presented here refute these mechanisms of Pin1 action. Using NMR, isothermal calorimetry (ITC), and small angle x-ray scattering (SAXS), we dissect binding and catalysis on multiple phosphorylated tau with particular emphasis toward the Alzheimer's associated AT180 tau epitope containing phosphorylated THR231 and SER235. We find that phosphorylated (p-) SER235-PRO, but not pTHR231-PRO, is exclusively catalyzed by full-length Pin1 and isolated PPIase domain. Importantly, site-specific measurements of Pin1-catalysis of CDK2/CycA-phosphorylated full-length tau reveal a number of sites that are catalyzed simultaneously with different efficiencies. Furthermore, we show that the turnover efficiency at pSER235 by Pin1 is independent of both the WW domain and phosphorylation on THR231. Our mechanistic results on site-specific binding and catalysis together with the lack of an increase of dephosphorylation rates by PP2A counter a series of previously published models for the role of Pin1 catalysis of tau in Alzheimer's disease. Together, our data reemphasize the complicated scenario between binding and catalysis of multiple phosphorylated tau by Pin1 and the need for directly linking biological phenotypes and residue-specific turnover in Pin1 substrates.
authors.affiliation: Howard Hughes Medical Institute and Department of Biochemistry, Brandeis University, Waltham, 02454, MA, USA.
authors.forename: Timo
authors.initials: T
authors.lastname: Eichner
authors.affiliation: Howard Hughes Medical Institute and Department of Biochemistry, Brandeis University, Waltham, 02454, MA, USA.
authors.forename: Steffen
authors.initials: S
authors.lastname: Kutter
authors.affiliation: Howard Hughes Medical Institute and Department of Biochemistry, Brandeis University, Waltham, 02454, MA, USA.
authors.forename: Wladimir
authors.initials: W
authors.lastname: Labeikovsky
authors.affiliation: Howard Hughes Medical Institute and Department of Biochemistry, Brandeis University, Waltham, 02454, MA, USA.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Buosi
authors.affiliation: Howard Hughes Medical Institute and Department of Biochemistry, Brandeis University, Waltham, 02454, MA, USA. Electronic address: dkern@brandeis.edu.
authors.forename: Dorothee
authors.initials: D
authors.lastname: Kern
mesh_terms: D002151:Calorimetry; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D000072340:NIMA-Interacting Peptidylprolyl Isomerase / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D053838:Scattering, Small Angle; D016875:tau Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: C054369:MAPT protein, human; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D016875:tau Proteins; C000606846:PIN1 protein, human
keywords: binding kinetics; molecular mechanism; peptidyl-prolyl cis/trans isomerization; phosphorylation; protein dynamics