title: Expression of microRNA-210 in tissue and serum of renal carcinoma patients and its effect on renal carcinoma cell proliferation, apoptosis, and invasion.
abstract: This study investigated the expression of microRNA-210 (miR-210) in tissue and serum of renal carcinoma patients and its effect on renal carcinoma cell proliferation, apoptosis, and invasion. Thirty-two renal carcinoma patients in our hospital were selected as the study group and 32 people receiving a physical examination were selected as the control group. miR-210 expression in the serum of renal carcinoma patients and in healthy subjects was quantified by real-time polymerase chain reaction. After miR-210 overexpression and inhibition in ACHN cells in human renal carcinoma, ACHN cell proliferation, apoptosis, and invasion were detected by CCK-8, flow cytometry, and a transwell invasion assay. The expression of miR-210 was significantly higher in renal carcinoma than in corresponding paracarcinoma tissues (P < 0.001). The expression of miR-210 was significantly higher in the serum of renal carcinoma than in the control group (P < 0.001). ACHN cell proliferation and invasion were significantly increased and apoptosis was significantly decreased (P < 0.05) when miR-210 was overexpressed. ACHN cell proliferation and invasion were significantly decreased and apoptosis was significantly increased (P < 0.05) when miR-210 was inhibited. In conclusion, miR-210 was highly expressed in tissues and serum of renal carcinoma patients. miR-210 could promote the proliferation and invasion of renal carcinoma cells and inhibit the apoptosis of renal cell carcinoma cells.
authors.affiliation: Department of Urology, Zhumadian Central Hospital, Zhumadian, China.
authors.forename: T Y
authors.initials: TY
authors.lastname: Liu
authors.affiliation: Department of Nursing, Huanghuai University, Zhumadian, China.
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Minor Operation Room, Zhumadian Central Hospital, Zhumadian, China.
authors.forename: S M
authors.initials: SM
authors.lastname: Du
authors.affiliation: Department of Urology, Zhumadian Central Hospital, Zhumadian, China.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Urology, Zhumadian Central Hospital, Zhumadian, China.
authors.forename: X H
authors.initials: XH
authors.lastname: Wen
mesh_terms: D000368:Aged; D017209:Apoptosis; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D015854:Up-Regulation
chemical_list: C543800:MIRN210 microRNA, human; D035683:MicroRNAs