title: Mitochondrial energy-dissipation pathway and cellular redox disruption compromises Arabidopsis resistance to turnip crinkle virus infection.
abstract: Members of the plant mitochondrial energy-dissipation pathway (MEDP) coordinate cellular energy metabolism, redox homeostasis and the balance of ROS production. However, the roles of MEDP members, particularly uncoupling protein (UCP), in resistance to turnip crinkle virus infection (TCV) are poorly understood. Here, we showed that disrupting some MEDP genes compromises plant resistance to TCV viral infection and this is partly associated with damaged photosynthetic characteristics, altered cellular redox and increased ROS production. Experiments using mutant plants with impaired cellular compartment redox poising further demonstrated that impaired chloroplast/mitochondria and cystosol redox increases the susceptibility of plants to viral infection. Our results illustrate a mechanism by which MEDP and cellular compartment redox act in concert to regulate plant resistance to viral infections.
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource & Eco-Environment and Plant Physiology Laboratory, State Key Laboratory of Hydraulics and Mountain River Engineering, College of Life Science, Sichuan University, Chengdu 610064, China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Pu
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource & Eco-Environment and Plant Physiology Laboratory, State Key Laboratory of Hydraulics and Mountain River Engineering, College of Life Science, Sichuan University, Chengdu 610064, China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Li
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource & Eco-Environment and Plant Physiology Laboratory, State Key Laboratory of Hydraulics and Mountain River Engineering, College of Life Science, Sichuan University, Chengdu 610064, China.
authors.forename: Li-Jie
authors.initials: LJ
authors.lastname: Wei
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource & Eco-Environment and Plant Physiology Laboratory, State Key Laboratory of Hydraulics and Mountain River Engineering, College of Life Science, Sichuan University, Chengdu 610064, China.
authors.forename: De-Hui
authors.initials: DH
authors.lastname: Xi
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource & Eco-Environment and Plant Physiology Laboratory, State Key Laboratory of Hydraulics and Mountain River Engineering, College of Life Science, Sichuan University, Chengdu 610064, China. Electronic address: hhlin@scu.edu.cn.
authors.forename: Hong-Hui
authors.initials: HH
authors.lastname: Lin
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000502:physiology* / Q000821:virology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001205:Ascorbic Acid / Q000235:genetics / Q000378:metabolism; D019184:Carmovirus / Q000502:physiology*; D002736:Chloroplasts / Q000378:metabolism / Q000821:virology; D017343:Genes, Plant; D005978:Glutathione / Q000235:genetics / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism / Q000821:virology*; D010084:Oxidation-Reduction; D010788:Photosynthesis; D010935:Plant Diseases / Q000235:genetics / Q000821:virology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; D017382:Reactive Oxygen Species; D005978:Glutathione; D001205:Ascorbic Acid
keywords: Alternative oxidase; Cyanide-resistant respiration; Proton gradient regulation 5; Redox homeostasis; Turnip crinkle virus; Uncoupling protein