title: Transcriptome analysis of phycocyanin inhibitory effects on SKOV-3 cell proliferation.
abstract: Phycocyanin (PC) from Spirulina platensis has inhibitory effects on tumor cell growth. In this research, the transcriptome study was designed to investigate the underlying molecular mechanisms of PC inhibition on human ovarian cancer cell SKOV-3 proliferation. The PC IC50 was 216.6μM and 163.8μM for 24h and 48h exposure, respectively, as determined by CCK-8 assay. The morphological changes of SKOV-3 cells after PC exposure were recorded using HE staining. Cells arrested in G2/M stages as determined by flow cytometry. The transcriptome analysis showed that 2031 genes (with > three-fold differences) were differentially expressed between the untreated and the PC-treated cells, including 1065 up-regulated and 966 down-regulated genes. Gene ontology and KEGG pathway analysis identified 18 classical pathways that were remarkably enriched, such as neurotrophin signaling pathway, VEGF signaling pathway and P53 signaling pathway. qPCR results further showed that PTPN12, S100A2, RPL26, and LAMA3 increased while HNRNPA1P10 decreased in PC-treated cells. Molecules and genes in those pathways may be potential targets to develop treatments for ovarian cancer.
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ying
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325035, China; The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325035, China; The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Ji
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325035, China; The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Chaoqing
authors.initials: C
authors.lastname: Lin
authors.affiliation: 118 Hospital of PLA, Wenzhou 325000, China.
authors.forename: Ruowang
authors.initials: R
authors.lastname: Pan
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan, China; University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Song
authors.affiliation: 118 Hospital of PLA, Wenzhou 325000, China.
authors.forename: Enyong
authors.initials: E
authors.lastname: Zhang
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Ren
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Jishun
authors.initials: J
authors.lastname: Chen
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Teng
authors.initials: T
authors.lastname: Xu
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Huiguang
authors.initials: H
authors.lastname: Yi
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325035, China. Electronic address: baoqy@genomics.cn.
authors.forename: Qiyu
authors.initials: Q
authors.lastname: Bao
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325035, China; The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China. Electronic address: hyl@wmu.edu.cn.
authors.forename: Yunliang
authors.initials: Y
authors.lastname: Hu
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325035, China. Electronic address: lpz@wmu.edu.cn.
authors.forename: Peizhen
authors.initials: P
authors.lastname: Li
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002630:Chemotactic Factors / Q000096:biosynthesis; D005260:Female; D005434:Flow Cytometry; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects*; D020869:Gene Expression Profiling; D006801:Humans; D007797:Laminin / Q000096:biosynthesis; D009414:Nerve Growth Factors / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000473:pathology; D010798:Phycocyanin / Q000494:pharmacology*; D054594:Protein Tyrosine Phosphatase, Non-Receptor Type 12 / Q000096:biosynthesis; D011509:Proteoglycans / Q000378:metabolism; D012269:Ribosomal Proteins / Q000096:biosynthesis; D009418:S100 Proteins / Q000096:biosynthesis; D015398:Signal Transduction / Q000187:drug effects; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D002630:Chemotactic Factors; D007797:Laminin; D009414:Nerve Growth Factors; D011509:Proteoglycans; C489216:RPL26 protein, human; D012269:Ribosomal Proteins; D009418:S100 Proteins; C098997:S100A2 protein, human; D016159:Tumor Suppressor Protein p53; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D010798:Phycocyanin; C095567:laminin alpha 3; C516738:PTPN12 protein, human; D054594:Protein Tyrosine Phosphatase, Non-Receptor Type 12
keywords: Cell proliferation; Phycocyanin; RNA-seq; SKOV-3 cell; qRT-PCR