title: Interleukin-33 facilitates neutrophil recruitment and bacterial clearance in S. aureus-caused peritonitis.
abstract: Interleukin (IL)-33, a newly recognized member of IL-1 family of cytokines, plays an important role in polarizing Th2-associated immunity. Recently growing evidence indicates that IL-33 also represents a crucial mediator of antimicrobial infection. In this study, we investigated the effect of IL-33 on antibacterial response using an acute Staphylococcus aureus peritoneal infection model. Our results showed that IL-33 administration induced a rapid bacterial clearance and markedly reduced the S. aureus infection-related mortality. IL-33-treated mice displayed increased neutrophil influx into the focus of infection and higher concentrations of chemokine CXCL2 in the peritoneum than untreated mice. The beneficial effect of IL-33 priming was related to reversal of the S. aureus-induced reduction of CXCR2 expression on the surface of neutrophils. Furthermore, conditioning of neutrophils by IL-33 led to the enhancement of complement receptor 3 expression induced by S. aureus, which in turn facilitates the phagocytosis of opsonized S. aureus. Finally, neutrophils primed by IL-33 upregulated the production of reactive oxygen species and the subsequent killing activity for S. aureus. All together, these findings suggest that IL-33, through regulating multiple steps of neutrophil-mediated bactericidal function, provides a profound effect in host antimicrobial defense response.
authors.affiliation: Department of Microbiology and Immunology, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Lan
authors.affiliation: Department of Microbiology and Immunology, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Baohong
authors.initials: B
authors.lastname: Yuan
authors.affiliation: Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Microbiology and Immunology, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Luo
authors.affiliation: Department of Microbiology and Immunology, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Huang
authors.affiliation: School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China. Electronic address: lyjche@163.com.
authors.forename: Yunjun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Intensive Care Unit, the First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510080, China.
authors.forename: Liangcheng
authors.initials: L
authors.lastname: Dai
authors.affiliation: Department of Microbiology and Immunology, Guangdong Pharmaceutical University, Guangzhou 510006, China; Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou 510006, China. Electronic address: huiyin0103@163.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Yin
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D000067596:Interleukin-33 / Q000276:immunology* / Q000627:therapeutic use; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D020556:Neutrophil Infiltration; D010538:Peritonitis / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D010587:Phagocytosis; D013203:Staphylococcal Infections / Q000276:immunology* / Q000382:microbiology
chemical_list: C506535:Il33 protein, mouse; D000067596:Interleukin-33
keywords: Interleukin-33; Neutrophils; Peritonitis; Phagocytosis; Reactive oxygen species