title: Characterization of Arabidopsis thaliana FLAVONOL SYNTHASE 1 (FLS1) -overexpression plants in response to abiotic stress.
abstract: Flavonoids are an important group of secondary metabolites that are involved in plant growth and contribute to human health. Many studies have focused on the biosynthesis pathway, biochemical characters, and biological functions of flavonoids. In this report, we showed that overexpression of FLS1 (FLS1-OX) not only altered seed coat color (resulting in a light brown color), but also affected flavonoid accumulation. Whereas fls1-3 mutants accumulated higher anthocyanin levels, FLS1-OX seedlings had lower levels than those of the wild-type. Besides, shoot tissues of FLS1-OX plants exhibited lower flavonol levels than those of the wild-type. However, growth performance and abiotic stress tolerance of FLS1-OX, fls1-3, and wild-type plants were not significantly different. Taken together, FLS1 can be manipulated (i.e., silenced or overexpressed) to redirect the flavonoid biosynthetic pathway toward anthocyanin production without negative effects on plant growth and development.
authors.affiliation: Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Seongbuk-gu, Seoul 136-713, Republic of Korea; Institute of Life Science and Natural Resources, Korea University, Seoul 136-713, Republic of Korea.
authors.forename: Nguyen Hoai
authors.initials: NH
authors.lastname: Nguyen
authors.affiliation: Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Seongbuk-gu, Seoul 136-713, Republic of Korea.
authors.forename: Jun Hyeok
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Seongbuk-gu, Seoul 136-713, Republic of Korea.
authors.forename: Jaeyoung
authors.initials: J
authors.lastname: Kwon
authors.affiliation: Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Seongbuk-gu, Seoul 136-713, Republic of Korea; Institute of Life Science and Natural Resources, Korea University, Seoul 136-713, Republic of Korea.
authors.forename: Chan Young
authors.initials: CY
authors.lastname: Jeong
authors.affiliation: Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Seongbuk-gu, Seoul 136-713, Republic of Korea.
authors.forename: Wonje
authors.initials: W
authors.lastname: Lee
authors.affiliation: Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Seongbuk-gu, Seoul 136-713, Republic of Korea.
authors.forename: Dongho
authors.initials: D
authors.lastname: Lee
authors.affiliation: Department of Molecular Biotechnology, College of Agriculture and Life Sciences, Bioenergy Research Center, Chonnam National University, Gwangju, Republic of Korea.
authors.forename: Suk-Whan
authors.initials: SW
authors.lastname: Hong
authors.affiliation: Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Seongbuk-gu, Seoul 136-713, Republic of Korea; Institute of Life Science and Natural Resources, Korea University, Seoul 136-713, Republic of Korea. Electronic address: lhojoung@korea.ac.kr.
authors.forename: Hojoung
authors.initials: H
authors.lastname: Lee
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D000872:Anthocyanins / Q000378:metabolism; D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D053898:Biosynthetic Pathways; D005419:Flavonoids / Q000378:metabolism*; D035264:Flowers / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D036226:Seedlings / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D012639:Seeds / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D013312:Stress, Physiological
chemical_list: D000872:Anthocyanins; D005419:Flavonoids; D010937:Plant Growth Regulators; D010940:Plant Proteins; D000040:Abscisic Acid; D010088:Oxidoreductases; C085313:flavonol synthase
keywords: Abiotic stress; Abscisic acid (ABA); Anthocyanin; Arabidopsis thaliana; FLS1; Flavonol