title: Self-assembled and covalently linked capillary coating of diazoresin and cyclodextrin-derived dendrimer for analysis of proteins by capillary electrophoresis.
abstract: Self-assembled and covalently linked capillary coatings of cyclodextrin-derived (CD) dendrimer were prepared using photosensitive diazoresin (DR) as a coupling agent. Layer by layer (LBL) self-assembled DR/CD-dendrimer coatings based on ionic bonding was fabricated first on the inner surface of capillary, and subsequently converted into covalent bonding after treatment with UV light through a unique photochemistry reaction of DR. Protein adsorption on the inner surface of capillary was suppressed by the DR/CD-dendrimer coating, and thus a baseline separation of lysozyme (Lys), myoglobin (Mb), bovine serum albumin (BSA) and ribonuclease A (RNase A) was achieved using capillary electrophoresis (CE). Compared with the bare capillary, the DR/CD-dendrimer covalently linked capillary coatings showed excellent protein separation performance with good stability and repeatability. Because of the replacement of highly toxic and moisture sensitive silane coupling agent by DR in the covalent coating preparation, this method may provide an environmentally friendly and simple way to prepare the covalently coated capillaries for CE.
authors.affiliation: Laboratory for New Fiber Materials and Modern Textile, Growing Base for State Key Laboratory, College of Materials Science and Engineering, Qingdao University, Qingdao 266071, PR China. Electronic address: yubingqdu@yahoo.com.
authors.forename: Bing
authors.initials: B
authors.lastname: Yu
authors.affiliation: Laboratory for New Fiber Materials and Modern Textile, Growing Base for State Key Laboratory, College of Materials Science and Engineering, Qingdao University, Qingdao 266071, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Chi
authors.affiliation: Key Laboratory of Biomass Chemical Engineering of Ministry of Education and Center for Bionanoengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, PR China.
authors.forename: Yuxing
authors.initials: Y
authors.lastname: Han
authors.affiliation: Laboratory for New Fiber Materials and Modern Textile, Growing Base for State Key Laboratory, College of Materials Science and Engineering, Qingdao University, Qingdao 266071, PR China. Electronic address: hailincong@yahoo.com.
authors.forename: Hailin
authors.initials: H
authors.lastname: Cong
authors.affiliation: Key Laboratory of Biomass Chemical Engineering of Ministry of Education and Center for Bionanoengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, PR China.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Tang
authors.affiliation: Laboratory for New Fiber Materials and Modern Textile, Growing Base for State Key Laboratory, College of Materials Science and Engineering, Qingdao University, Qingdao 266071, PR China.
authors.forename: Qiaohong
authors.initials: Q
authors.lastname: Peng
mesh_terms: D000818:Animals; D001391:Azo Compounds / Q000737:chemistry*; D002417:Cattle; D003505:Cyclodextrins / Q000737:chemistry*; D050091:Dendrimers / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D055668:Photochemical Processes; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D014466:Ultraviolet Rays
chemical_list: D001391:Azo Compounds; D003505:Cyclodextrins; D050091:Dendrimers; D011506:Proteins; C504851:diazoresin
keywords: CE; Capillary coatings; Dendrimer; Diazoresin; Protein