title: Solid-phase extraction of flavonoids in honey samples using carbamate-embedded triacontyl-modified silica sorbent.
abstract: In this study, carbamate-embedded triacontyl-modified silica (Sil-CBM-C30) is successfully prepared and used as an efficient sorbent for solid-phase extraction. The extraction performance of the resultant sorbent is evaluated with five flavonoids including myricetin, quercetin, luteolin, kaempferol and isorhamnetin. Main parameters, which affect extraction efficiencies, are carefully investigated and optimized. Comparative experiments between Sil-CBM-C30 and commercial C18 sorbents indicate that the extraction efficiencies of the former one surpass the latter one. The modification of carbamate-embedded triacontyl group on surface of silica causes analytes extracted by hydrophobic, hydrogen bonding and π-π interactions. Under optimal conditions, good linearities and satisfied LODs and LOQs are achieved. The SPE-HPLC-DAD method is successfully developed and applied for the honey sample analysis.
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China; University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Houmei
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China; University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Mingliang
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China; State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China. Electronic address: hdqiu@licp.cas.cn.
authors.forename: Hongdeng
authors.initials: H
authors.lastname: Qiu
mesh_terms: D002219:Carbamates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D005504:Food Analysis; D006722:Honey / Q000032:analysis*; D044949:Kaempferols / Q000302:isolation & purification; D057230:Limit of Detection; D047311:Luteolin / Q000302:isolation & purification; D011794:Quercetin / Q000031:analogs & derivatives / Q000302:isolation & purification; D012822:Silicon Dioxide / Q000737:chemistry; D052616:Solid Phase Extraction
chemical_list: D002219:Carbamates; D005419:Flavonoids; D044949:Kaempferols; C047368:3-methylquercetin; C006552:kaempferol; D012822:Silicon Dioxide; C040015:myricetin; D011794:Quercetin; D047311:Luteolin
keywords: Carbamate; Flavonoids; Honey sample; Solid phase extraction; Triacontyl group