title: Preconcentration of valsartan by dispersive liquid-liquid microextraction based on solidification of floating organic drop and its determination in urine sample: Central composite design.
abstract: In this work, a fast, easy, and efficient dispersive liquid-liquid microextraction method based on solidification of floating organic drop followed by high-performance liquid chromatography with UV detection was developed for the separation/preconcentration and determination of the drug valsartan. Experimental design was applied for the optimization of the effective variables (such as volume of extracting and dispersing solvents, ionic strength, and pH) on the extraction efficiency of valsartan from urine samples. The optimized values were 250.0 μL ethanol, 65.0 μL 1-dodecanol, 4.0% w/v NaCl, pH 3.8, 1.0 min extraction time, and 4.0 min centrifugation at 4000 rpm min(-1) . The linear response (r(2) = 0.997) was obtained in the range of 0.013-10.0 μg mL(-1) with a limit of detection of 4.0 ng mL(-1) and relative standard deviations of less than 5.0 % (n = 6).
authors.affiliation: Department of Chemistry, Faculty of Science, Yazd University, Yazd, Iran.
authors.forename: Arezou Amiri
authors.initials: AA
authors.lastname: Pebdani
authors.affiliation: Department of Chemistry, Faculty of Science, Yazd University, Yazd, Iran.
authors.forename: Ali Mohammad Haji
authors.initials: AM
authors.lastname: Shabani
authors.affiliation: Department of Chemistry, Faculty of Science, Yazd University, Yazd, Iran.
authors.forename: Shayesteh
authors.initials: S
authors.lastname: Dadfarnia
authors.affiliation: Medicinal plants Research Center, Yasuj University of Medical Science, Yasuj, Iran.
authors.forename: Mohammad Sharif
authors.initials: MS
authors.lastname: Talebianpoor
authors.affiliation: Department of Chemistry, Khatam Alanbia University of Technology, Behbahan, Iran.
authors.forename: Saeid
authors.initials: S
authors.lastname: Khodadoust
mesh_terms: D002851:Chromatography, High Pressure Liquid; D064368:Healthy Volunteers; D006801:Humans; D059627:Liquid Phase Microextraction / Q000295:instrumentation; D010316:Particle Size; D012107:Research Design; D012984:Software; D014466:Ultraviolet Rays; D000068756:Valsartan / Q000737:chemistry / Q000302:isolation & purification* / Q000652:urine*
chemical_list: D000068756:Valsartan
keywords: Central composite design; Microextraction; Solidification; Urine; Valsartan