title: In vivo acoustic and photoacoustic focusing of circulating cells.
abstract: In vivo flow cytometry using vessels as natural tubes with native cell flows has revolutionized the study of rare circulating tumor cells in a complex blood background. However, the presence of many blood cells in the detection volume makes it difficult to count each cell in this volume. We introduce method for manipulation of circulating cells in vivo with the use of gradient acoustic forces induced by ultrasound and photoacoustic waves. In a murine model, we demonstrated cell trapping, redirecting and focusing in blood and lymph flow into a tight stream, noninvasive wall-free transportation of blood, and the potential for photoacoustic detection of sickle cells without labeling and of leukocytes targeted by functionalized nanoparticles. Integration of cell focusing with intravital imaging methods may provide a versatile biological tool for single-cell analysis in circulation, with a focus on in vivo needleless blood tests, and preclinical studies of human diseases in animal models.
authors.affiliation: Arkansas Nanomedicine Center, University of Arkansas for Medical Sciences (UAMS), Little Rock, Arkansas 72205.
authors.forename: Ekaterina I
authors.initials: EI
authors.lastname: Galanzha
authors.affiliation: Arkansas Nanomedicine Center, University of Arkansas for Medical Sciences (UAMS), Little Rock, Arkansas 72205.
authors.forename: Mark G
authors.initials: MG
authors.lastname: Viegas
authors.affiliation: Bauman Moscow State Technical University, Moscow, Russia, 107005.
authors.forename: Taras I
authors.initials: TI
authors.lastname: Malinsky
authors.affiliation: Moscow Institute of Physics and Technology (MIPT), Moscow Region, 141700, Russia.
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Melerzanov
authors.affiliation: Arkansas Nanomedicine Center, University of Arkansas for Medical Sciences (UAMS), Little Rock, Arkansas 72205.
authors.forename: Mazen A
authors.initials: MA
authors.lastname: Juratli
authors.affiliation: Arkansas Nanomedicine Center, University of Arkansas for Medical Sciences (UAMS), Little Rock, Arkansas 72205.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Sarimollaoglu
authors.affiliation: Arkansas Nanomedicine Center, University of Arkansas for Medical Sciences (UAMS), Little Rock, Arkansas 72205.
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Nedosekin
authors.affiliation: Arkansas Nanomedicine Center, University of Arkansas for Medical Sciences (UAMS), Little Rock, Arkansas 72205.|Moscow Institute of Physics and Technology (MIPT), Moscow Region, 141700, Russia.
authors.forename: Vladimir P
authors.initials: VP
authors.lastname: Zharov
mesh_terms: D000162:Acoustics / Q000295:instrumentation*; D000755:Anemia, Sickle Cell / Q000097:blood / Q000000981:diagnostic imaging / Q000235:genetics; D000818:Animals; D001808:Blood Vessels / Q000166:cytology / Q000502:physiology; D045744:Cell Line, Tumor; D004912:Erythrocytes / Q000473:pathology; D005434:Flow Cytometry; D006801:Humans; D007834:Lasers; D007962:Leukocytes / Q000166:cytology; D008819:Mice, Nude; D008822:Mice, Transgenic; D053758:Nanoparticles; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D061088:Photoacoustic Techniques / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D059010:Single-Cell Analysis / Q000295:instrumentation* / Q000379:methods; D014159:Transducers; D014465:Ultrasonics