title: Ribosomal protein L7 as a suitable reference gene for quantifying gene expression in gastropod Bellamya aeruginosa.
abstract: Expression levels of eight candidate reference genes were quantified in tissues of gastropod Bellamya aeruginosa exposed for 10 d to various stressors, including fasting, 17β-estradiol, 17α-methyltestosterone, and Cd(2+). The results showed that 18s rRNA was the most highly expressed of the candidate reference genes, while H2A was the least expressed. There were no significant changes (p>0.05) in the expression of the eight genes in tissues among the different treatments. Using RefFinder to evaluate the expression stabilities of the eight candidate reference genes, ribosomal protein was shown to be the most stable reference gene, and no effects were observed among the different stressor treatments. These results indicate that RPL 7 is the most suitable reference gene for quantifying gene expression in B. aeruginosa under environmental stress, which was verified in B. aeruginosa exposed to high doses of E2 for 24 and 72h.
authors.affiliation: College of Resources and Civil Engineering, Northeastern University, Shenyang 110819, China; State Environmental Protection Key Laboratory of Estuarine and Coastal Research, Chinese Research Academy of Environment Sciences, Beijing 100012, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: State Environmental Protection Key Laboratory of Estuarine and Coastal Research, Chinese Research Academy of Environment Sciences, Beijing 100012, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Lei
authors.affiliation: State Environmental Protection Key Laboratory of Estuarine and Coastal Research, Chinese Research Academy of Environment Sciences, Beijing 100012, China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Ma
authors.affiliation: State Environmental Protection Key Laboratory of Estuarine and Coastal Research, Chinese Research Academy of Environment Sciences, Beijing 100012, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Qiao
authors.affiliation: State Environmental Protection Key Laboratory of Estuarine and Coastal Research, Chinese Research Academy of Environment Sciences, Beijing 100012, China.
authors.forename: Zi-Cheng
authors.initials: ZC
authors.lastname: Li
authors.affiliation: State Environmental Protection Key Laboratory of Estuarine and Coastal Research, Chinese Research Academy of Environment Sciences, Beijing 100012, China. Electronic address: anlhui@163.com.
authors.forename: Li-Hui
authors.initials: LH
authors.lastname: An
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods; D049851:Gastropoda / Q000235:genetics*; D015870:Gene Expression / Q000502:physiology*; D012269:Ribosomal Proteins / Q000235:genetics*; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D015415:Biomarkers; D012269:Ribosomal Proteins
keywords: Gastropod; Gene expression; Real-time qPCR; Reference gene; Ribosomal protein L 7