title: Involvement of an SRF-MADS protein McmA in regulation of extracellular enzyme production and asexual/sexual development in Aspergillus nidulans.
abstract: SRF-MADS proteins are transcription factors conserved among eukaryotes that regulate a variety of cellular functions; however, their physiological roles are still not well understood in filamentous fungi. Effects of a mutation in mcmA gene that encodes the sole SRF-MADS protein in the fungus Aspergillus nidulans were examined by RNA sequencing. Sequencing data revealed that expression levels of cellulase genes were significantly decreased by the mutation as reported previously. However, expression levels of various hemicellulolytic enzyme genes, several extracellular protease genes, the nosA and rosA genes involved in sexual development, and AN4394 encoding an ortholog of EcdR involved in Aspergillus oryzae conidiation, were also significantly decreased by the mutation. As expected from the RNA sequencing data, the mcmA mutant had reduced protease production, cleistothecial development, and conidiation. This is the first report describing the involvement of SRF-MADS proteins in protease production in fungi, and asexual and sexual development in Aspergillus.
authors.forename: Nuo
authors.initials: N
authors.lastname: Li
authors.affiliation: a Department of Biological Mechanisms and Functions , Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya , Japan.
authors.forename: Emi
authors.initials: E
authors.lastname: Kunitake
authors.affiliation: a Department of Biological Mechanisms and Functions , Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya , Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Endo
authors.affiliation: a Department of Biological Mechanisms and Functions , Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya , Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Aoyama
authors.affiliation: a Department of Biological Mechanisms and Functions , Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya , Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Kanamaru
authors.affiliation: a Department of Biological Mechanisms and Functions , Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya , Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kimura
authors.affiliation: a Department of Biological Mechanisms and Functions , Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya , Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Kato
authors.affiliation: a Department of Biological Mechanisms and Functions , Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya , Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Kobayashi
mesh_terms: D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D001236:Aspergillus oryzae / Q000235:genetics; D002480:Cellulase / Q000096:biosynthesis / Q000235:genetics*; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D059014:High-Throughput Nucleotide Sequencing; D026161:MADS Domain Proteins / Q000235:genetics*; D009154:Mutation; D012100:Reproduction, Asexual / Q000235:genetics*; D046468:Sexual Development / Q000235:genetics; D013172:Spores, Fungal / Q000201:enzymology / Q000235:genetics / Q000254:growth & development
chemical_list: D005656:Fungal Proteins; D026161:MADS Domain Proteins; D002480:Cellulase
keywords: asexual development; cellulase; hemicellulase; protease; sexual development