title: Leukocyte responses to immobilized patterns of CXCL8.
abstract: The attachment of neutrophils to the endothelial surface and their migration towards the site of inflammation following chemokine gradients play an essential role in the innate immune response. Chemokines adhere to glycosaminoglycans on the endothelial surface to be detected by leukocytes and trigger their movement along surface- bound gradients in a process called haptotaxis. In assays to systematically study the response of leukocytes to surface-bound compounds both the spatial arrangement of the compound as well as the mode of immobilization need to be controlled. In this study microcontact printing was employed to create patterns of hydrophobic or functionalized thiols on gold-coated glass slides and CXCL8 was immobilized on the thiol coated areas using three different strategies. Human neutrophils adhered to the CXCL8-coated lines but not to the PEG-coated background. We could show that more cells adhered to CXCL8 adsorbed to hydrophobic octadecanethiol than on CXCL8 covalently bound to amino undecanethiol or CXCL8 specifically bound to immobilized heparin on aminothiol. Likewise general cell activity such as lamellipodia formation and random migration were most pronounced for CXCL8 adsorbed on a hydrophobic surface which may be attributed to the larger amounts of protein immobilized on this type of surface.
authors.affiliation: Karlsruhe Institute of Technology, Center for Functional Nanostructures, Haid-und-Neu-Straße 6, 76131 Karlsruhe, Germany; Karlsruhe Institute of Technology, Institute of Functional Interfaces, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Girrbach
authors.affiliation: Technische Universität Darmstadt, Clemens-Schöpf-Institut für Organische Chemie und Biochemie, Alarich-Weiss-Str. 8, 64287 Darmstadt, Germany.
authors.forename: Ina
authors.initials: I
authors.lastname: Rink
authors.affiliation: Karlsruhe Institute of Technology, Institute of Functional Interfaces, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Ladnorg
authors.affiliation: Karlsruhe Institute of Technology, Institute of Functional Interfaces, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
authors.forename: Carlos
authors.initials: C
authors.lastname: Azucena
authors.affiliation: Karlsruhe Institute of Technology, Institute of Functional Interfaces, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Heißler
authors.affiliation: Max-Planck-Institute for Intelligent Systems, Heisenbergstr. 3, 70569 Stuttgart, Germany; Biophysical Chemistry, University of Heidelberg, Im Neuenheimer Feld 253, 69120 Heidelberg, Germany, Germany.
authors.forename: Tamás
authors.initials: T
authors.lastname: Haraszti
authors.affiliation: Karlsruhe Institute of Technology, Institute of Toxicology and Genetics, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
authors.forename: Ute
authors.initials: U
authors.lastname: Schepers
authors.affiliation: Technische Universität Darmstadt, Clemens-Schöpf-Institut für Organische Chemie und Biochemie, Alarich-Weiss-Str. 8, 64287 Darmstadt, Germany. Electronic address: schmitz@biochemie.tu-darmstadt.de.
authors.forename: Katja
authors.initials: K
authors.lastname: Schmitz
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D005898:Glass / Q000737:chemistry; D006046:Gold / Q000737:chemistry* / Q000494:pharmacology; D006493:Heparin / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D055767:Immobilized Proteins / Q000737:chemistry / Q000494:pharmacology*; D016209:Interleukin-8 / Q000737:chemistry / Q000494:pharmacology*; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D061251:Primary Cell Culture; D011327:Printing; D013438:Sulfhydryl Compounds / Q000737:chemistry* / Q000494:pharmacology; D013499:Surface Properties
chemical_list: C508606:CXCL8 protein, human; D055767:Immobilized Proteins; D016209:Interleukin-8; D013438:Sulfhydryl Compounds; D011092:Polyethylene Glycols; D006046:Gold; D006493:Heparin
keywords: Biofunctional surfaces; Chemokines; Microcontact printing; Protein patterns