title: [Proteomics study on ficolin 3 in the human plasma of type 2 diabetics].
abstract: OBJECTIVE
  To explore the expression trend of ficolin 3 (FCN3) in type 2 diabetes (T2DM) plasma.
  
  
  METHODS
  Two-dimensional polyacrylamide gel electrophoresis (2DE) was used to separate the plasma proteins from T2DM patients and healthy control subjects. MALDI-TOF-TOF was used to identify the differential proteins. Western Blot and enzyme-linked immune response (ELISA) were used to verify the results from 2DE.
  
  
  RESULTS
  The experiment on 2DE showed complement C1s subcomponent, complement C3, C9 and FCN3 were up-regulated in the plasma of T2DM patients. The Western Blot results showed that C9 and FCN3 increased in the plasma of T2DM patients with respect to T2DM-free subjects. The experiment results on ELISA showed that the plasma concentration of FCN3 were (50.88 ± 3.85 ) and (36.20 ± 2.75 ) μg/mL (P = 0.0062) in T2DM and T2DM-free, respectively. The plasma concentration of C9 were (26.22 ± 1.43) and (19.23 ± 1.55) μg/mL (P = 0.0022) in T2DM and T2DM-free. FCN3 and C9 were up-regulated in T2DM plasma.
  
  
  CONCLUSIONS
  FCN3 over-expressed in the plasma of T2DM patients,which activated the complement system in greater degree.
authors.forename: Qinggang
authors.initials: Q
authors.lastname: Shang
authors.forename: Liru
authors.initials: L
authors.lastname: Feng
authors.forename: Wei
authors.initials: W
authors.lastname: Yu
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
mesh_terms: D001798:Blood Proteins / Q000378:metabolism*; D015153:Blotting, Western; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D037102:Lectins / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D000097604:Ficolins
chemical_list: D001798:Blood Proteins; D037102:Lectins; D000097604:Ficolins