title: Peripheral blood collection: the first step towards gene expression profiling.
abstract: A crucial challenge for gene expression analysis in human biomonitoring studies on whole blood samples is rapid sample handling and mRNA stabilization. This study was designed to evaluate the impact of short bench times (less than 30 min) on yield, quality and gene expression of mRNA in the presence of different stabilization buffers (Tempus(TM) Blood RNA tube and RNAlater(®) Stabilization Reagent). Microarray analyzes showed significant changes over short periods of time in expression of a considerate part of the transcriptome (2356 genes) with a prominent role for NFкB-, cancer- and glucocorticoid-mediated networks, and specifically interleukin-8 (IL-8). These findings suggest that even short bench times affect gene expression, requiring to carry out blood collection in a strictly standardized way.
authors.affiliation: a Unit Environmental Risk and Health, Flemish Institute for Technological Research (VITO NV) , Mol , Belgium ;|b Department of Biomedical Sciences ;
authors.forename: Carmen
authors.initials: C
authors.lastname: Franken
authors.affiliation: a Unit Environmental Risk and Health, Flemish Institute for Technological Research (VITO NV) , Mol , Belgium ;|c Department of Epidemiology and Social Medicine , University of Antwerp , Antwerp , Belgium ;
authors.forename: Sylvie
authors.initials: S
authors.lastname: Remy
authors.affiliation: a Unit Environmental Risk and Health, Flemish Institute for Technological Research (VITO NV) , Mol , Belgium ;
authors.forename: Nathalie
authors.initials: N
authors.lastname: Lambrechts
authors.affiliation: a Unit Environmental Risk and Health, Flemish Institute for Technological Research (VITO NV) , Mol , Belgium ;
authors.forename: Karen
authors.initials: K
authors.lastname: Hollanders
authors.affiliation: a Unit Environmental Risk and Health, Flemish Institute for Technological Research (VITO NV) , Mol , Belgium ;
authors.forename: Elly
authors.initials: E
authors.lastname: Den Hond
authors.affiliation: a Unit Environmental Risk and Health, Flemish Institute for Technological Research (VITO NV) , Mol , Belgium ;|b Department of Biomedical Sciences ;|d Department of Environmental Medicine , University of Southern Denmark , Odense , Denmark.
authors.forename: Greet
authors.initials: G
authors.lastname: Schoeters
mesh_terms: D001800:Blood Specimen Collection / Q000379:methods* / Q000592:standards; D002021:Buffers; D020869:Gene Expression Profiling / Q000379:methods* / Q000592:standards; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics; D046228:Microarray Analysis; D012333:RNA, Messenger / Q000096:biosynthesis; D013997:Time Factors; D059467:Transcriptome / Q000235:genetics
chemical_list: D002021:Buffers; C508606:CXCL8 protein, human; D016209:Interleukin-8; D012333:RNA, Messenger
keywords: Bench time; IL-8; RNA stability; biomarker; human blood sampling