title: Graphene Functionalized Scaffolds Reduce the Inflammatory Response and Supports Endogenous Neuroblast Migration when Implanted in the Adult Brain.
abstract: Electroactive materials have been investigated as next-generation neuronal tissue engineering scaffolds to enhance neuronal regeneration and functional recovery after brain injury. Graphene, an emerging neuronal scaffold material with charge transfer properties, has shown promising results for neuronal cell survival and differentiation in vitro. In this in vivo work, electrospun microfiber scaffolds coated with self-assembled colloidal graphene, were implanted into the striatum or into the subventricular zone of adult rats. Microglia and astrocyte activation levels were suppressed with graphene functionalization. In addition, self-assembled graphene implants prevented glial scarring in the brain 7 weeks following implantation. Astrocyte guidance within the scaffold and redirection of neuroblasts from the subventricular zone along the implants was also demonstrated. These findings provide new functional evidence for the potential use of graphene scaffolds as a therapeutic platform to support central nervous system regeneration.
authors.affiliation: Department of Materials Science and Engineering, Monash Institute of Medical Engineering, Monash University, Melbourne, VIC, Australia.|Australian Regenerative Medicine Institute, Monash University, Melbourne, VIC, Australia.|The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Melbourne, VIC, Australia.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhou
authors.affiliation: Department of Materials Science and Engineering, Monash Institute of Medical Engineering, Monash University, Melbourne, VIC, Australia.
authors.forename: Sepideh
authors.initials: S
authors.lastname: Motamed
authors.affiliation: The Ritchie Centre, Hudson Institute of Medical Research, Monash University, Melbourne, VIC, Australia.
authors.forename: George A
authors.initials: GA
authors.lastname: Thouas
authors.affiliation: Australian Regenerative Medicine Institute, Monash University, Melbourne, VIC, Australia.
authors.forename: Claude C
authors.initials: CC
authors.lastname: Bernard
authors.affiliation: Department of Materials Science and Engineering, Monash Institute of Medical Engineering, Monash University, Melbourne, VIC, Australia.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Physiology, Monash University, Melbourne, VIC, Australia.
authors.forename: Helena C
authors.initials: HC
authors.lastname: Parkington
authors.affiliation: Department of Physiology, Monash University, Melbourne, VIC, Australia.
authors.forename: Harold A
authors.initials: HA
authors.lastname: Coleman
authors.affiliation: The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Melbourne, VIC, Australia.
authors.forename: David I
authors.initials: DI
authors.lastname: Finkelstein
authors.affiliation: Department of Materials Science and Engineering, Monash Institute of Medical Engineering, Monash University, Melbourne, VIC, Australia.
authors.forename: John S
authors.initials: JS
authors.lastname: Forsythe
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects; D002921:Cicatrix / Q000517:prevention & control*; D020099:Coated Materials, Biocompatible / Q000633:toxicity; D003102:Colloids; D003342:Corpus Striatum / Q000293:injuries / Q000473:pathology* / Q000502:physiology; D025802:Electric Capacitance; D004553:Electric Conductivity; D005549:Foreign-Body Reaction / Q000209:etiology / Q000517:prevention & control*; D005911:Gliosis / Q000517:prevention & control*; D006108:Graphite / Q000008:administration & dosage / Q000494:pharmacology*; D007249:Inflammation; D020547:Lateral Ventricles / Q000293:injuries / Q000473:pathology* / Q000502:physiology; D008297:Male; D008422:Materials Testing; D017628:Microglia / Q000187:drug effects / Q000502:physiology; D055616:Microtechnology; D049329:Nanostructures / Q000008:administration & dosage* / Q000009:adverse effects; D009416:Nerve Regeneration / Q000187:drug effects*; D058953:Neural Stem Cells / Q000166:cytology*; D055495:Neurogenesis / Q000187:drug effects*; D019736:Prostheses and Implants / Q000009:adverse effects*; D051381:Rats; D017208:Rats, Wistar; D054457:Tissue Scaffolds / Q000009:adverse effects
chemical_list: D020099:Coated Materials, Biocompatible; D003102:Colloids; D006108:Graphite