title: Maliponia aquimaris gen. nov., sp. nov., isolated from seawater.
abstract: A Gram-stain-negative, coccoid- or oval-shaped and non-motile bacterial strain, designated MM-10T, belonging to the Alphaproteobacteria, was isolated from seawater of the Yellow Sea, Korea, and was subjected to a polyphasic taxonomic study. Strain MM-10T grew optimally at pH 7.0-8.0, at 30 °C and in the presence of 2-3 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain MM-10T fell within the clade comprising members of the genera Mameliella, Sagittula, Antarctobacter and Ponticoccus. Strain MM-10T exhibited a 16S rRNA gene sequence similarity of 97.7 % with respect to the type strain of Mameliella alba. The DNA G+C content of strain MM-10T was 66.0 mol% and its mean level of DNA-DNA relatedness with M. alba LMG 24665T was 12.3±1.4 % . Strain MM-10T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The major polar lipids were phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. Differential phenotypic properties, together with phylogenetic distinctiveness, demonstrate that strain MM-10T is distinguishable from recognized species of the genera Mameliella, Sagittula and Antarctobacter. On the basis of the data presented, strain MM-10T is considered to represent a novel species of a new genus, for which the name Maliponia aquimaris gen. nov., sp. nov. is proposed. The type strain of Maliponia aquimaris is MM-10T (=KCTC 42721T=CECT 8898T).
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.|University of Science and Technology (UST), 113 Gwahak-ro, Yuseong-gu, Daejeon, South Korea.
authors.forename: Yong-Taek
authors.initials: YT
authors.lastname: Jung
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, South Korea.
authors.forename: Jung-Sook
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C024989:coenzyme Q10