title: The role of immunohistochemical adrenomedullin and Bcl-2 expression in development of type-1 endometrial adenocarcinoma: Adrenomedullin expression in endometrium.
abstract: BACKGROUND
  Adrenomedullin (AM) is a pluripotent peptide first discovered from human pheochromocytoma. AM expression has been shown in various cancer types including endometrium cancer. Bcl-2 is an antiapoptotic protein which might be regulated by AM in hypoxic conditions. The aim of the present study was to investigate the role of AM and Bcl-2 expressions in carcinogenesis of type-1 endometrium cancer.
  
  
  METHODS
  Study group consisted of 10 proliferative endometrium, 22 simple endometrial hyperplasia, 23 endometrial intraepithelial neoplasia (EIN) and 30 Grade 1 endometrioid adenocarcinoma patients. AM and Bcl-2 expressions were investigated by immunohistochemistry.
  
  
  RESULTS
  Mean AM Allred score was 3±2.6, 5.6±1.6 and 5.7±2.5 in benign, EIN and adenocarcinoma groups, respectively. AM expression was significantly higher in EIN and adenocarcinoma groups than in benign endometrium group (p<0.05). Mean Bcl-2 Allred score was 6.4±2.1, 5.2±2.6, 2.3±2 in benign endometrium, EIN and adenocarcinoma groups, respectively. Mean Bcl-2 Allred score was similar between benign endometrium and EIN groups (p>0.05). However, it was significantly lower in adenocarcinoma group (p<0.05). An inverse correlation between AM and Bcl-2 expressions was found (r: -0.4, p<0.001).
  
  
  CONCLUSIONS
  Our findings showed that AM expression increased in progression from benign endometrium to EIN and type-1 adenocarcinoma while expression of Bcl-2 decreased in transition from EIN to carcinoma.
authors.affiliation: Süleyman Demirel University, Faculty of Medicine, Department of Pathology, Isparta, Turkey. Electronic address: kemalkbozkurt@hotmail.com.
authors.forename: Kemal Kürşat
authors.initials: KK
authors.lastname: Bozkurt
authors.affiliation: Suleyman Demirel University, Faculty of Medicine, Department of Gynecologic Oncology, Isparta, Turkey. Electronic address: dryakupyalcin@gmail.com.
authors.forename: Yakup
authors.initials: Y
authors.lastname: Yalçın
authors.affiliation: Isparta Maternity Hospital, Department of Obstetrics and Gynecology, Isparta, Turkey. Electronic address: ebru.md@hotmail.com.
authors.forename: Ebru
authors.initials: E
authors.lastname: Erdemoğlu
authors.affiliation: Suleyman Demirel University, Faculty of Medicine, Department of Gynecologic Oncology, Isparta, Turkey. Electronic address: buraktatar@yahoo.com.
authors.forename: Burak
authors.initials: B
authors.lastname: Tatar
authors.affiliation: Suleyman Demirel University, Faculty of Medicine, Department of Gynecologic Oncology, Isparta, Turkey. Electronic address: evrimmd@yahoo.com.
authors.forename: Evrim
authors.initials: E
authors.lastname: Erdemoğlu
authors.affiliation: Suleyman Demirel University, Faculty of Medicine, Department of Nuclear Medicine, Isparta, Turkey. Electronic address: sureyyacerci@hotmail.com.
authors.forename: Sevim Süreyya
authors.initials: SS
authors.lastname: Çerçi
authors.affiliation: Süleyman Demirel University, Faculty of Medicine, Department of Pathology, Isparta, Turkey. Electronic address: metin@metinciris.com.tr.
authors.forename: İbrahim Metin
authors.initials: İM
authors.lastname: Çiriş
authors.affiliation: Süleyman Demirel University, Faculty of Medicine, Department of Pathology, Isparta, Turkey. Electronic address: sirinbaspinar@gmail.com.
authors.forename: Şirin
authors.initials: Ş
authors.lastname: Başpınar
authors.affiliation: Süleyman Demirel University, Faculty of Medicine, Department of Pathology, Isparta, Turkey. Electronic address: afifenalinci@hotmail.com.
authors.forename: Afife
authors.initials: A
authors.lastname: Uğuz
authors.affiliation: Acıbadem Maslak Hospital, Department of Pathology, Istanbul, Turkey. Electronic address: nilgunkap@hotmail.com.
authors.forename: Nilgün
authors.initials: N
authors.lastname: Kapucuoğlu
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D053607:Adrenomedullin / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018450:Disease Progression; D004714:Endometrial Hyperplasia / Q000378:metabolism / Q000473:pathology; D016889:Endometrial Neoplasms / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D060787:Neoplasm Grading; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D053607:Adrenomedullin
keywords: Adenocarcinoma; Adrenomedullin; Bcl-2; EIN; Endometrium