title: Analysis of telomerase target gene expression effects from murine models in patient cohorts by homology translation and random survival forest modeling.
abstract: Acute myeloid leukemia (AML) is an aggressive and rapidly fatal blood cancer that affects patients of any age group. Despite an initial response to standard chemotherapy, most patients relapse and this relapse is mediated by leukemia stem cell (LSC) populations. We identified a functional requirement for telomerase in sustaining LSC populations in murine models of AML and validated this requirement using an inhibitor of telomerase in human AML. Here, we describe in detail the contents, quality control and methods of the gene expression analysis used in the published study (Gene Expression Omnibus GSE63242). Additionally, we provide annotated gene lists of telomerase regulated genes in AML and R code snippets to access and analyze the data used in the original manuscript.
authors.affiliation: The Finsen Laboratory, Bioinformatics Centre at Department of Biology, and Biotech Research and Innovation Center (BRIC), University of Copenhagen, 2200 Copenhagen N, Denmark.
authors.forename: Frederik Otzen
authors.initials: FO
authors.lastname: Bagger
authors.affiliation: Division of Immunology, QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia.
authors.forename: Claudia
authors.initials: C
authors.lastname: Bruedigam
authors.affiliation: Division of Immunology, QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia; University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Steven W
authors.initials: SW
authors.lastname: Lane
keywords: AML; Leukemia; Stem cells; Telomerase; Telomere