title: Doxorubicin loaded 17β-estradiol based SWNT dispersions for target specific killing of cancer cells.
abstract: The present work reports the synthesis of a 17β-estradiol based amphiphiles comprising of polyethylene glycol (PEG) moiety linked through succinic acid that non-covalently dispersed (76%) the single walled carbon nanotubes (SWNTs) in water. The superior exfoliation of carbon nanotubes was characterized by microscopic and spectroscopic studies. Significant stability of these SWNT dispersions was observed in the presence of protein in cell culture media and the nanohybrids were highly biocompatible toward mammalian cells. Anticancer drug doxorubicin loaded on these nanohybrids was selectively delivered within estrogen receptor rich cancer cells, MCF7 (breast cancer cell) and A549 (lung cancer cell). Microscopic studies showed the localization of doxorubicin within the cancer cell nucleus whereas no such localization was observed in ER negative cells. Both these ER positive cancer cells were killed by ∼3 fold higher efficiency than that of ER negative MDA-MB-231 (advanced breast cancer cell) and HeLa cells that are deprived of estrogen receptors. Thus, judiciously designed estradiol based nanohybrids proved to be excellent tool for SWNT dispersion and also for selectively killing of ER positive cancer cells. To the best of our knowledge, for the first time non-covalently modified SWNTs by estradiol based amphiphilic dispersing agent have been used for selective killing of ER positive cancer cells by doxorubicin loaded on dispersed SWNTs. It holds immense promise to be exploited as a cancer therapeutic agent.
authors.affiliation: Department of Biological Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Kolkata 700 032, India.
authors.forename: Moumita
authors.initials: M
authors.lastname: Ghosh
authors.affiliation: Department of Biological Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Kolkata 700 032, India. Electronic address: bcpkd@iacs.res.in.
authors.forename: Prasanta Kumar
authors.initials: PK
authors.lastname: Das
mesh_terms: D000072283:A549 Cells; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D002467:Cell Nucleus / Q000187:drug effects; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology*; D004337:Drug Carriers; D004339:Drug Compounding; D004958:Estradiol / Q000737:chemistry* / Q000378:metabolism; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D058990:Molecular Targeted Therapy; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009928:Organ Specificity; D011092:Polyethylene Glycols / Q000737:chemistry*; D011485:Protein Binding; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism; D019802:Succinic Acid / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D037742:Nanotubes, Carbon; D011960:Receptors, Estrogen; D013501:Surface-Active Agents; D011092:Polyethylene Glycols; D004958:Estradiol; D004317:Doxorubicin; D019802:Succinic Acid
keywords: Amphiphiles; Carbon nanotube; Dispersion; Doxorubicin; Estradiol; Non-covalent; Target specific delivery