title: Establishment and characterization of a canine soft tissue sarcoma patient-derived xenograft model.
abstract: Spontaneously occurring soft tissue sarcoma (STS) is relatively common in canine cancer patients. Because of the similarities to human disease, canine STSs are a valuable and readily available resource for the study of new therapeutics. In this study, a canine patient-derived xenograft (PDX) model, CDX-STS2, was established. The CDX-STS2 model was engrafted and expanded for systemic administration studies with chemotherapeutic agents commonly used to treat STS, including doxorubicin, docetaxel and gemcitabine. Immunohistochemistry for drug-specific biomarkers and tumour growth measurement revealed tumour sensitivity to doxorubicin and docetaxel, whereas gemcitabine had no effect on tumour growth. Although many human PDX tumour models have been established, relatively few canine PDX models have been reported to date. CDX-STS2 represents a new STS PDX research model of canine origin that will be useful in bridging preclinical research with clinical studies of STS in pet dogs.
authors.affiliation: Presage Biosciences, Seattle, WA, USA.
authors.forename: J P
authors.initials: JP
authors.lastname: Frazier
authors.affiliation: Presage Biosciences, Seattle, WA, USA.
authors.forename: E
authors.initials: E
authors.lastname: Beirne
authors.affiliation: Presage Biosciences, Seattle, WA, USA.
authors.forename: S H
authors.initials: SH
authors.lastname: Ditzler
authors.affiliation: Presage Biosciences, Seattle, WA, USA.
authors.forename: I
authors.initials: I
authors.lastname: Tretyak
authors.affiliation: Presage Biosciences, Seattle, WA, USA.
authors.forename: J R
authors.initials: JR
authors.lastname: Casalini
authors.affiliation: Presage Biosciences, Seattle, WA, USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Thirstrup
authors.affiliation: Department of Veterinary Biosciences, The Ohio State University, Columbus, OH, USA.
authors.forename: S
authors.initials: S
authors.lastname: Knoblaugh
authors.affiliation: Specialty VetPath, Shoreline, WA, USA.
authors.forename: J G
authors.initials: JG
authors.lastname: Ward
authors.affiliation: Veterinary Cancer Specialty Care, Lynnwood, WA, USA.
authors.forename: C D
authors.initials: CD
authors.lastname: Tripp
authors.affiliation: Presage Biosciences, Seattle, WA, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Klinghoffer
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D014408:Biomarkers, Tumor; D004195:Disease Models, Animal; D004285:Dogs; D064593:Heterografts; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation / Q000379:methods / Q000662:veterinary*; D012509:Sarcoma / Q000188:drug therapy / Q000473:pathology / Q000662:veterinary*; D012513:Sarcoma, Experimental / Q000188:drug therapy* / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor
keywords: comparative oncology; in vitro models; mouse models; oncology; pathology; tumor biology