title: Secretion and Reversible Assembly of Extracellular-like Matrix by Enzyme-Active Colloidosome-Based Protocells.
abstract: The secretion and reversible assembly of an extracellular-like matrix by enzyme-active inorganic protocells (colloidosomes) is described. Addition of N-fluorenyl-methoxycarbonyl-tyrosine-(O)-phosphate to an aqueous suspension of alkaline phosphatase-containing colloidosomes results in molecular uptake and dephosphorylation to produce a time-dependent sequence of supramolecular hydrogel motifs (outer membrane wall, cytoskeletal-like interior and extra-protocellular matrix) that are integrated and remodelled within the microcapsule architecture and surrounding environment. Heat-induced disassembly of the extra-protocellular matrix followed by cooling produces colloidosomes with a densely packed hydrogel interior. These procedures are exploited for the fabrication of nested colloidosomes with spatially delineated regions of hydrogelation.
authors.affiliation: Centre for Protolife Research and Centre for Organized Matter Chemistry, School of Chemistry, University of Bristol , Bristol BS8 1TS, United Kingdom.
authors.forename: Khrongkhwan
authors.initials: K
authors.lastname: Akkarachaneeyakorn
authors.affiliation: Centre for Protolife Research and Centre for Organized Matter Chemistry, School of Chemistry, University of Bristol , Bristol BS8 1TS, United Kingdom.
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.affiliation: Centre for Protolife Research and Centre for Organized Matter Chemistry, School of Chemistry, University of Bristol , Bristol BS8 1TS, United Kingdom.
authors.forename: Sean A
authors.initials: SA
authors.lastname: Davis
authors.affiliation: Centre for Protolife Research and Centre for Organized Matter Chemistry, School of Chemistry, University of Bristol , Bristol BS8 1TS, United Kingdom.
authors.forename: Stephen
authors.initials: S
authors.lastname: Mann
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry*; D000818:Animals; D058045:Artificial Cells / Q000737:chemistry*; D006690:Bisbenzimidazole; D002417:Cattle; D003102:Colloids; D005456:Fluorescent Dyes; D020100:Hydrogels / Q000737:chemistry*; D044367:Phase Transition; D013696:Temperature; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D003102:Colloids; D005456:Fluorescent Dyes; D020100:Hydrogels; C000614792:N-fluorenyl-methoxycarbonyl-tyrosine-(O)-phosphate; D014443:Tyrosine; D000469:Alkaline Phosphatase; D006690:Bisbenzimidazole