title: Functional phosphorylation sites in cardiac myofilament proteins are evolutionarily conserved in skeletal myofilament proteins.
abstract: Protein phosphorylation plays an important role in regulating cardiac contractile function, but phosphorylation is not thought to play a regulatory role in skeletal muscle. To examine how myofilament phosphorylation arose in the human heart, we analyzed the amino acid sequences of 25 cardiac phosphorylation sites in animals ranging from fruit flies to humans. These analyses indicated that of the 25 human phosphorylation sites examined, 11 have been conserved across vertebrates and four have been sporadically present in vertebrates. Furthermore, all 11 of the cardiac sites found across vertebrates were present in skeletal muscle isoforms, along with three sites that were sporadically present. Based on the conservation of amino acid sequences between cardiac and skeletal contractile proteins, we tested for phosphorylation in mammalian skeletal muscle using several biochemical techniques and found evidence that multiple myofilament proteins were phosphorylated. Several of these phosphorylation sites were validated using mass spectrometry, including one site that is present in slow- and fast-twitch troponin I (TnI), but was lost in cardiac TnI. Thus, several myofilament phosphorylation sites present in the human heart likely arose in invertebrate muscle, have been evolutionarily conserved in skeletal muscle, and potentially have functional effects in both skeletal and cardiac muscle.
authors.affiliation: Department of Integrative Biology, University of California, Berkeley, Berkeley, California; and Department of Biochemistry and Molecular Biology, Oregon Health & Science University, Portland, Oregon grossse@ohsu.edu.
authors.forename: Sean M
authors.initials: SM
authors.lastname: Gross
authors.affiliation: Department of Integrative Biology, University of California, Berkeley, Berkeley, California; and.
authors.forename: Steven L
authors.initials: SL
authors.lastname: Lehman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019143:Evolution, Molecular; D006321:Heart / Q000502:physiology*; D006801:Humans; D018482:Muscle, Skeletal / Q000378:metabolism*; D009200:Myocardial Contraction / Q000235:genetics; D009206:Myocardium / Q000378:metabolism*; D009210:Myofibrils / Q000235:genetics* / Q000378:metabolism*; D010766:Phosphorylation / Q000235:genetics*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D020318:Rats, Long-Evans; D016415:Sequence Alignment; D019210:Troponin I / Q000235:genetics / Q000378:metabolism
chemical_list: D020033:Protein Isoforms; D019210:Troponin I
keywords: myofilament proteins; phosphorylation; skeletal muscle