title: Inhibition of platelet aggregation by vanilloid-like agents is not mediated by transient receptor potential vanilloid-1 channels or cannabinoid receptors.
abstract: Vanilloid-like agents, including capsaicin, N-arachidonoyl-dopamine and N-oleoyldopamine inhibit platelet aggregation, however little is known about the precise mechanism(s) of action. The authors have previously shown that blocking of the capsaicin receptor, transient receptor potential vanilloid-1 (TRPV1), does not interfere with capsaicin action during adenosine diphosphate (ADP)-induced aggregation. This research is extended to investigate the effect of these vanilloid-like-agents on platelet count, and to test whether the effect of these agents is mediated through TRPV1 and/or cannabinoid (CB1 and CB2) receptors in the presence of other agonists, including collagen and arachidonic acid. Incubation of platelets with each of the individual vanilloids, or with receptor antagonists of TRPV1 (SB452533), CB1 (AM251) and CB2 (AM630), for up to 2 h did not significantly affect the platelet count. Similarly, the effect of individual vanilloids on the inhibition of platelet aggregation was not significantly different in the presence of receptor agonists compared to control, irrespective of the agonist used, suggesting that the inhibitory effect of vanilloids on platelet aggregation is independent of TRPV1, CB1 and CB2 receptors. Further research on the antiplatelet activity of vanilloids should focus on mechanisms other than those associated with vanilloid receptors.
authors.affiliation: School of Health Sciences, University of Tasmania, Launceston, Tasmania, Australia.
authors.forename: Safa
authors.initials: S
authors.lastname: Almaghrabi
authors.affiliation: School of Health Sciences, University of Tasmania, Launceston, Tasmania, Australia.
authors.forename: Dominic
authors.initials: D
authors.lastname: Geraghty
authors.affiliation: School of Health Sciences, University of Tasmania, Launceston, Tasmania, Australia.
authors.forename: Kiran
authors.initials: K
authors.lastname: Ahuja
authors.affiliation: School of Health Sciences, University of Tasmania, Launceston, Tasmania, Australia.
authors.forename: Murray
authors.initials: M
authors.lastname: Adams
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D063386:Cannabinoid Receptor Agonists / Q000494:pharmacology*; D063387:Cannabinoid Receptor Antagonists / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010974:Platelet Aggregation / Q000187:drug effects / Q000502:physiology*; D050916:TRPV Cation Channels / Q000819:agonists / Q000037:antagonists & inhibitors / Q000502:physiology*; D055815:Young Adult
chemical_list: D063386:Cannabinoid Receptor Agonists; D063387:Cannabinoid Receptor Antagonists; D050916:TRPV Cation Channels; C494482:TRPV1 protein, human
keywords: N-arachidonoyl-dopamine; N-oleoyldopamine; cannabinoid; capsaicin; transient receptor potential vanilloid-1