title: Correlation Between the Clinical Parameters and Tissue Phenotype in Patients Affected by Deep-Infiltrating Endometriosis.
abstract: The current study aimed to identify and validate an applicable immunohistochemistry panel including Ki-67, c-MYC, estrogen receptor-α (ER-α), and progesterone receptor isoforms A/B (PR-A/B) in correlation with clinicopathological parameters in patients affected by deep infiltrating endometriosis. Tissue microarrays were prepared from a cohort of 113 patients. Phenotypic profile of the panel molecules was evaluated in glands and stroma in parallel with microvessels and stroma density measurements. Principal component analysis was performed on 8 immunohistochemical variables, 2 histological variables, and 8 subgroups of clinical parameters. The immunohistochemical profiling showed consistent Ki-67 immunostaining in 17.9% of the samples and c-MYC in 83.1%, while intense ER-α immunoreactivity was detected in 84% of the samples and PR-A/B isoforms in 24.1% of them. The combination of clinical parameters and tissue phenotype allowed a stratification of endometriosis-affected patients. Such novel phenotypical and clinical correlation could be helpful in the future studies for a better stratification of the disease aiming at a personalized patient care.
authors.affiliation: Cochin Institute, Inserm U1016, CNRS 8104, Université Paris Descartes, Paris, France.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Vinci
authors.affiliation: Department of Pathology, Assistance Publique-Hôpitaux de Paris, Hôpitaux Universitaires Paris Centre, Cochin Hospital, Paris, France.
authors.forename: Sylviane
authors.initials: S
authors.lastname: Arkwright
authors.affiliation: Department of Pathology, Assistance Publique-Hôpitaux de Paris, Hôpitaux Universitaires Paris Centre, Cochin Hospital, Paris, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Audebourg
authors.affiliation: Department of Pathology, Assistance Publique-Hôpitaux de Paris, Hôpitaux Universitaires Paris Centre, Cochin Hospital, Paris, France.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Radenen
authors.affiliation: Department of Gynecology Obstetrics II and Reproductive Medicine, Faculté de Médecine, AP-HP, Groupe Hospitalier Ouest, Centre Hospitalier Universitaire Paris Centre, Paris, France.
authors.forename: Charles
authors.initials: C
authors.lastname: Chapron
authors.affiliation: Department of Gynecology Obstetrics II and Reproductive Medicine, Faculté de Médecine, AP-HP, Groupe Hospitalier Ouest, Centre Hospitalier Universitaire Paris Centre, Paris, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Borghese
authors.affiliation: Service de Chirurgie Digestive Hépatobiliaire et Endocrinienne Faculté de Médecine, AP-HP, Groupe Hospitalier Ouest, Centre Hospitalier Universitaire Paris Centre, Paris, France.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Dousset
authors.affiliation: Cochin Institute, Inserm U1016, CNRS 8104, Université Paris Descartes, Paris, France.
authors.forename: Celine
authors.initials: C
authors.lastname: Mehats
authors.affiliation: Cochin Institute, Inserm U1016, CNRS 8104, Université Paris Descartes, Paris, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Vaiman
authors.affiliation: Department of Pathology, Assistance Publique-Hôpitaux de Paris, Hôpitaux Universitaires Paris Centre, Cochin Hospital, Paris, France.
authors.forename: Marie-Cécile
authors.initials: MC
authors.lastname: Vacher-Lavenu
authors.affiliation: Cochin Institute, Inserm U1016, CNRS 8104, Université Paris Descartes, Paris, France jean.gogusev@gmail.com.
authors.forename: Jean
authors.initials: J
authors.lastname: Gogusev
mesh_terms: D000328:Adult; D004268:DNA-Binding Proteins / Q000378:metabolism; D004715:Endometriosis / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology*; D047628:Estrogen Receptor alpha / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D055806:Microvessels / Q000378:metabolism; D010641:Phenotype; D011980:Receptors, Progesterone / Q000378:metabolism; D046888:Tissue Array Analysis; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D019394:Ki-67 Antigen; C116115:MYCBP protein, human; D011980:Receptors, Progesterone; D014157:Transcription Factors; C101118:progesterone receptor A; C097314:progesterone receptor B
keywords: DIE; IHC; PCA; TMAs; deep infiltrating endometriosis; immunohistochemistry; principal component analysis; tissue microarrays