title: Purification and characterisation of processive-type endoglucanase and β-glucosidase from Aspergillus ochraceus MTCC 1810 through saccharification of delignified coir pith to glucose.
abstract: The study describes purification and characterisation of processive-type endoglucanase and β-glucosidase from Aspergillus ochraceus MTCC 1810 through bioconversion of delignified coir pith to fermentable glucose. The purified processive endoglucanase (AS-HT-Celuz A) and β-glucosidase (AS-HT-Celuz B) were found to have molecular mass of ≈78-kDa and 43-kDa respectively with optimum endoglucanase (35.63U/ml), total cellulase (28.15FPU/ml) and β-glucosidase (15.19U/ml) activities at 40°C/pH 6. The unique feature of AS-HT-Celuz A is the multiple substrate specificity and processivity towards both amorphous and crystalline cellulose. Zymogram indicated both endo and exoglucanase activities residing in different binding sites of a single protein exhibiting sequential synergy with its own β-glucosidase. Accordingly, the identified enzymes could be implemented as synergistic cellulases for complete cellulose saccharification which still considered an unresolved issue in bio-refineries.
authors.affiliation: National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Kochi, Kerala 682016, India.
authors.forename: P
authors.initials: P
authors.lastname: Asha
authors.affiliation: National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Kochi, Kerala 682016, India.
authors.forename: Jose
authors.initials: J
authors.lastname: Divya
authors.affiliation: National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Kochi, Kerala 682016, India. Electronic address: isbsingh@gmail.com.
authors.forename: I S
authors.initials: IS
authors.lastname: Bright Singh
mesh_terms: D001235:Aspergillus ochraceus / Q000201:enzymology / Q000378:metabolism*; D002480:Cellulase / Q000302:isolation & purification* / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism; D005285:Fermentation; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D008031:Lignin / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity; D001617:beta-Glucosidase / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; C507903:coir; D002482:Cellulose; D008031:Lignin; D001617:beta-Glucosidase; D002480:Cellulase; D005947:Glucose
keywords: Aspergillus ochraceus; Delignified coir pith; Glucose; Processive endoglucanase; β-Glucosidase