title: Circulating miR-130a, miR-27b, and miR-210 in Patients With Peripheral Artery Disease and Their Potential Relationship With Oxidative Stress.
abstract: Some emerging risk factors such as oxidative stress biomarkers and microRNAs (miRs) may add additional value to the established risk factors for peripheral artery disease (PAD). We enrolled 27 patients with PAD and 27 age-matched controls. We examined the levels of a series of miRs (miR-130a, miR-27b, and miR-210) in serum samples. The level of well-established oxidative stress biomarkers, such as lipid hydroperoxides, isoprostanes, hemeoxygenase-1 (HO-1) and reduced glutathione, was also measured in plasma and their relationship with the miRs was determined. Levels of miR-130a, miR-27b, and miR-210 were significantly increased in patients with PAD when compared to the controls. The level of miR-130 was positively correlated with body mass index, whereas miR-210 was inversely associated with pain-free walking distance (PfWD). None of the evaluated miRs was associated with lowered PfWD of patients with PAD (stage IIa > 250 m, IIb < 250 m) or oxidative stress parameters. In conclusion, our findings suggest the need for more research to assess if miRs can serve as useful markers for the early diagnosis and monitoring of PAD.
authors.affiliation: 1 Department of Clinical and Experimental Medicine, University of Catania, Catania, Italy.
authors.forename: Salvatore Santo
authors.initials: SS
authors.lastname: Signorelli
authors.affiliation: 2 Biomedical and Biotechnological Science, University of Catania, Catania, Italy.
authors.forename: Guido Li
authors.initials: GL
authors.lastname: Volsi
authors.affiliation: 3 Department of Experimental Biomedicine and Clinical Neuroscience, University of Palermo, Palermo, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Pitruzzella
authors.affiliation: 1 Department of Clinical and Experimental Medicine, University of Catania, Catania, Italy.
authors.forename: Valerio
authors.initials: V
authors.lastname: Fiore
authors.affiliation: 1 Department of Clinical and Experimental Medicine, University of Catania, Catania, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Mangiafico
authors.affiliation: 4 Department of Drug Science, University of Catania, Catania, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Vanella
authors.affiliation: 2 Biomedical and Biotechnological Science, University of Catania, Catania, Italy.
authors.forename: Rosalba
authors.initials: R
authors.lastname: Parenti
authors.affiliation: 5 Biomedical Department of Internal Medicine and Medical Specialties, University of Palermo, Palermo, Italy.
authors.forename: Manfredi
authors.initials: M
authors.lastname: Rizzo
authors.affiliation: 2 Biomedical and Biotechnological Science, University of Catania, Catania, Italy.
authors.forename: Giovanni Li
authors.initials: GL
authors.lastname: Volti
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000097:blood; D005260:Female; D005978:Glutathione / Q000097:blood; D051547:Heme Oxygenase-1 / Q000097:blood; D006801:Humans; D028421:Isoprostanes / Q000097:blood; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D058729:Peripheral Arterial Disease / Q000097:blood* / Q000175:diagnosis; D010865:Pilot Projects; D012307:Risk Factors; D013223:Statistics as Topic
chemical_list: D015415:Biomarkers; D028421:Isoprostanes; D008054:Lipid Peroxides; C554019:MIRN130 microRNA, human; C543800:MIRN210 microRNA, human; C531388:MIRN27 microRNA, human; D035683:MicroRNAs; D051547:Heme Oxygenase-1; D005978:Glutathione
keywords: Heme Oxygenase; biomarker; microRNA; oxidative stress; peripheral arterial disease