title: G-CSF Administration after the Intraosseous Infusion of Hypertonic Hydroxyethyl Starches Accelerating Wound Healing Combined with Hemorrhagic Shock.
abstract: OBJECTIVE
  To evaluate the therapeutic effects of G-CSF administration after intraosseous (IO) resuscitation in hemorrhagic shock (HS) combined with cutaneous injury rats.
  
  
  METHODS
  The rats were randomly divided into four groups: (1) HS with resuscitation (blank), (2) HS with resuscitation + G-CSF (G-CSF, 200 μg/kg body weight, subcutaneous injection), (3) HS with resuscitation + normal saline solution injection (normal saline), and (4) HS + G-CSF injection without resuscitation (Unres/G-CSF). To estimate the treatment effects, the vital signs of alteration were first evaluated, and then wound closure rates and homing of MSCs and EPCs to the wound skins and vasculogenesis were measured. Besides, inflammation and vasculogenesis related mRNA expressions were also examined.
  
  
  RESULTS
  IO infusion hypertonic hydroxyethyl starch (HHES) exhibited beneficial volume expansion roles and G-CSF administration accelerated wound healing 3 days ahead of other groups under hemorrhagic shock. Circulating and the homing of MSCs and EPCs at wound skins were significantly elevated at 6 h after G-CSF treatment. Inflammation was declined since 3 d while angiogenesis was more obvious in G-CSF treated group on day 9.
  
  
  CONCLUSIONS
  These results suggested that the synergistical application of HHES and G-CSF has life-saving effects and is beneficial for improving wound healing in HS combined with cutaneous injury rats.
authors.affiliation: Institute of Basic Medicine, Chinese PLA General Hospital, Beijing 100853, China; Medical College of Nankai University, Tianjin 300071, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Huang
authors.affiliation: Institute of Basic Medicine, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Jiejie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Basic Medicine, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Haojie
authors.initials: H
authors.lastname: Hao
authors.affiliation: Institute of Basic Medicine, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Tong
authors.affiliation: Institute of Basic Medicine, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Ti
authors.affiliation: Institute of Basic Medicine, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Huiling
authors.initials: H
authors.lastname: Liu
authors.affiliation: Institute of Basic Medicine, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Haijing
authors.initials: H
authors.lastname: Song
authors.affiliation: Institute of Basic Medicine, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Chaoguang
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Institute of Basic Medicine, Chinese PLA General Hospital, Beijing 100853, China; Burns Institute, First Affiliated Hospital of Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Fu
authors.affiliation: Institute of Basic Medicine, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Han
mesh_terms: D000818:Animals; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage*; D006801:Humans; D006895:Hydroxyethyl Starch Derivatives / Q000008:administration & dosage; D007249:Inflammation / Q000188:drug therapy* / Q000473:pathology; D017148:Infusions, Intraosseous; D051381:Rats; D012151:Resuscitation; D012771:Shock, Hemorrhagic / Q000188:drug therapy* / Q000473:pathology; D012867:Skin / Q000187:drug effects / Q000293:injuries / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D006895:Hydroxyethyl Starch Derivatives; D016179:Granulocyte Colony-Stimulating Factor