title: Cranberry Product Decreases Fat Accumulation in Caenorhabditis elegans.
abstract: Cranberry phenolic compounds have been linked to many health benefits. A recent report suggested that cranberry bioactives inhibit adipogenesis in 3T3-L1 adipocytes. Thus, we investigated the effects and mechanisms of the cranberry product (CP) on lipid metabolism using the Caenorhabditis elegans (C. elegans) model. CP (0.016% and 0.08%) dose-dependently reduced overall fat accumulation in C. elegans (N2, wild type) by 43% and 74%, respectively, without affecting its pumping rates or locomotive activities. CP decreased fat accumulation in aak-2 (an ortholog of AMP-activated kinase α) and tub-1 (an ortholog of TUBBY) mutants significantly, but only minimal effects were observed in sbp-1 (an ortholog of sterol response element-binding protein-1) and nhr-49 (an ortholog of peroxisome proliferator-activated receptor-α) mutant strains. We further confirmed that CP downregulated sbp-1, cebp, and hosl-1 (an ortholog of hormone-sensitive lipase homolog) expression, while increasing the expression of nhr-49 in wild-type C. elegans. These results suggest that CP could effectively reduce fat accumulation in C. elegans dependent on sbp-1, cebp, and nhr-49, but not aak-2 and tub-1.
authors.affiliation: 1 Department of Food Science, University of Massachusetts , Amherst, Massachusetts, USA .
authors.forename: Quancai
authors.initials: Q
authors.lastname: Sun
authors.affiliation: 1 Department of Food Science, University of Massachusetts , Amherst, Massachusetts, USA .
authors.forename: Yiren
authors.initials: Y
authors.lastname: Yue
authors.affiliation: 1 Department of Food Science, University of Massachusetts , Amherst, Massachusetts, USA .
authors.forename: Peiyi
authors.initials: P
authors.lastname: Shen
authors.affiliation: 2 Amherst Regional High School , Amherst, Massachusetts, USA.
authors.forename: Jeremy J
authors.initials: JJ
authors.lastname: Yang
authors.affiliation: 1 Department of Food Science, University of Massachusetts , Amherst, Massachusetts, USA .
authors.forename: Yeonhwa
authors.initials: Y
authors.lastname: Park
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects; D005223:Fats / Q000378:metabolism*; D005260:Female; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D029799:Vaccinium macrocarpon / Q000737:chemistry*
chemical_list: D029742:Caenorhabditis elegans Proteins; D005223:Fats; D010936:Plant Extracts
keywords: C. elegans; cranberry; fat accumulation; nhr-49; sbp-1