title: Fatty Acids Profile, Phenolic Compounds and Antioxidant Capacity in Elicited Callus of Thevetia peruviana (Pers.) K. Schum.
abstract: The aim of this study was analyze the effect of jasmonic acid (JA) and abscisic acid (ABA) as elicitors on fatty acids profile (FAP), phenolic compounds (PC) and antioxidant capacity (AC) in callus of Thevetia peruviana. Schenk & Hildebrandt (SH) medium, supplemented with 2 mg/L 2, 4-dichlorophenoxyacetic (2, 4-D) and 0.5 mg/L kinetin (KIN) was used for callus induction. The effect of JA (50, 75 and 100 μM) and ABA (10, 55 and 100 μM) on FAP, PC and AC were analyzed using a response surface design. A maximum of 2.8 mg/g of TPC was obtained with 100 plus 10 µM JA and ABA, respectively, whereas AC maximum (2.17 μg/mL) was obtained with 75 plus 100 µM JA and ABA, respectively. The FAP was affected for JA but not for ABA. JA increased cis-9, cis-12-octadecadienoic acid and decreased dodecanoic acid. Eight fatty acids were identified by GC-MS analysis and cis-9-octadecenoic acid (18:1) was the principal fatty acid reaching 76 % in treatment with 50 μM JA plus 55 μM ABA. In conclusion, JA may be used in T. peruviana callus culture for obtain oil with different fatty acids profile.
authors.affiliation: Tecnológico Nacional de México. Instituto Tecnológico de Tuxtla Gutiérrez, Laboratorio de Biotecnología vegetal.
authors.forename: Jack
authors.initials: J
authors.lastname: Rincón-Pérez
authors.forename: Ludwi
authors.initials: L
authors.lastname: Rodríguez-Hernández
authors.forename: Víctor Manuel
authors.initials: VM
authors.lastname: Ruíz-Valdiviezo
authors.forename: Miguel
authors.initials: M
authors.lastname: Abud-Archila
authors.forename: María Celina
authors.initials: MC
authors.lastname: Luján-Hidalgo
authors.forename: Nancy
authors.initials: N
authors.lastname: Ruiz-Lau
authors.forename: Daniel
authors.initials: D
authors.lastname: González-Mendoza
authors.forename: Federico Antonio
authors.initials: FA
authors.lastname: Gutiérrez-Miceli
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology*; D000085:Acetates; D000975:Antioxidants / Q000032:analysis*; D003517:Cyclopentanes / Q000494:pharmacology*; D005227:Fatty Acids / Q000032:analysis* / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry; D007701:Kinetin; D007850:Lauric Acids / Q000032:analysis; D054883:Oxylipins / Q000494:pharmacology*; D010636:Phenols / Q000032:analysis*; D013229:Stearic Acids / Q000032:analysis; D031052:Thevetia / Q000737:chemistry*
chemical_list: C114874:5-octadecenoic acid; D000085:Acetates; D000975:Antioxidants; D003517:Cyclopentanes; D005227:Fatty Acids; D007850:Lauric Acids; D054883:Oxylipins; D010636:Phenols; D013229:Stearic Acids; C030358:lauric acid; C011006:jasmonic acid; D000040:Abscisic Acid; D007701:Kinetin; C415326:phenoxyacetic acid