title: [BRCA1 associated protein 1 (BAP1) expression in pleural diffuse malignant mesothelioma: A comparative cytological and histological analyses on 50 patients].
abstract: BACKGROUND
  Diffuse malignant mesothelioma (MMD) is a rare disease. The diagnosis is difficult and needs an antibody panel. The tumor suppressor gene BRCA1 associated protein 1 (BAP1) is involved in several cancers, including MMD. Loss of BAP1 expression is correlated with BAP1 somatic or constitutional genetic defects. Our work assesses the value of integrating BAP1 in the panel of antibodies used for the diagnosis of MMD.
  
  
  METHODS
  Immunohistochemical techniques were performed on cytological and histological specimens of MMD and adenocarcinoma pleural metastasis.
  
  
  RESULTS
  Of the 26 patients with MMD and the 24 patients with adenocarcinoma pleural metastasis, loss of BAP1 expression was observed in 11 (48%) and one adenocarcinoma (6%) on cytological specimens and in 12 MMD (48%) and in one adenocarcinoma (5%) on biopsy specimens. The concordance between immunocytochemistry and immunohistochemistry was 100%. The specificity of BAP1 was 100% on cytological and biopsy specimen for the diagnosis of malignancy in case of mesothelial proliferation.
  
  
  CONCLUSIONS
  Loss of BAP1 expression is an indicator of MMD in a context of mesothelial proliferation. This immunohistochemistry could be integrated in the panel of immunostaining used for MMD diagnosis, either on histological or cytological samples. Furthermore, loss of BAP1 expression guides the patient to an oncology genetic counseling in order to eliminate a MMD developed as part of a constitutional genetic defect.
authors.affiliation: Service de pathologie et de neuropathologie Est, centre de biologie et de pathologie Est (CBPE), 59, boulevard Pinel, 69577 Bron cedex, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Jaouen
authors.affiliation: Service de pathologie et de neuropathologie Est, centre de biologie et de pathologie Est (CBPE), 59, boulevard Pinel, 69577 Bron cedex, France; Institut de cancérologie des hospices civils de Lyon (IC-HCL), 2, quai des Célestins, 69002 Lyon, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Thivolet-Bejui
authors.affiliation: Service de pathologie et de neuropathologie Est, centre de biologie et de pathologie Est (CBPE), 59, boulevard Pinel, 69577 Bron cedex, France; Institut de cancérologie des hospices civils de Lyon (IC-HCL), 2, quai des Célestins, 69002 Lyon, France.
authors.forename: Lara
authors.initials: L
authors.lastname: Chalabreysse
authors.affiliation: Service de pathologie et de neuropathologie Est, centre de biologie et de pathologie Est (CBPE), 59, boulevard Pinel, 69577 Bron cedex, France; Institut de cancérologie des hospices civils de Lyon (IC-HCL), 2, quai des Célestins, 69002 Lyon, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Piaton
authors.affiliation: Institut de cancérologie des hospices civils de Lyon (IC-HCL), 2, quai des Célestins, 69002 Lyon, France; Service d'anatomie et de cytologie pathologiques, centre hospitalier Lyon sud, chemin du Grand-Revoyet, 69495 Pierre-Bénite, France.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Traverse-Glehen
authors.affiliation: Institut de cancérologie des hospices civils de Lyon (IC-HCL), 2, quai des Célestins, 69002 Lyon, France; Service d'anatomie et de cytologie pathologiques, centre hospitalier Lyon sud, chemin du Grand-Revoyet, 69495 Pierre-Bénite, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Isaac
authors.affiliation: Institut de cancérologie des hospices civils de Lyon (IC-HCL), 2, quai des Célestins, 69002 Lyon, France; Service d'anatomie et de cytologie pathologiques, centre hospitalier Lyon sud, chemin du Grand-Revoyet, 69495 Pierre-Bénite, France.
authors.forename: Myriam
authors.initials: M
authors.lastname: Decaussin-Petrucci
authors.affiliation: Institut de cancérologie des hospices civils de Lyon (IC-HCL), 2, quai des Célestins, 69002 Lyon, France; Service d'anatomie et de cytologie pathologiques, centre hospitalier Lyon sud, chemin du Grand-Revoyet, 69495 Pierre-Bénite, France.
authors.forename: Laurianne
authors.initials: L
authors.lastname: Depaepe
authors.affiliation: Institut de cancérologie des hospices civils de Lyon (IC-HCL), 2, quai des Célestins, 69002 Lyon, France; Service d'anatomie et de cytologie pathologiques, centre hospitalier Lyon sud, chemin du Grand-Revoyet, 69495 Pierre-Bénite, France.
authors.forename: Juliette
authors.initials: J
authors.lastname: Fontaine
authors.affiliation: Service de pathologie et de neuropathologie Est, centre de biologie et de pathologie Est (CBPE), 59, boulevard Pinel, 69577 Bron cedex, France; Institut de cancérologie des hospices civils de Lyon (IC-HCL), 2, quai des Célestins, 69002 Lyon, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Remy
authors.affiliation: Institut de cancérologie des hospices civils de Lyon (IC-HCL), 2, quai des Célestins, 69002 Lyon, France; Service de chirurgie thoracique, hôpital Louis-Pradel, 59, boulevard Pinel, 69577 Bron, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Maury
authors.affiliation: Service de pathologie et de neuropathologie Est, centre de biologie et de pathologie Est (CBPE), 59, boulevard Pinel, 69577 Bron cedex, France; Institut de cancérologie des hospices civils de Lyon (IC-HCL), 2, quai des Célestins, 69002 Lyon, France. Electronic address: marie.brevet01@chu-lyon.fr.
authors.forename: Marie
authors.initials: M
authors.lastname: Brevet
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D001706:Biopsy; D003937:Diagnosis, Differential; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D005817:Genetic Counseling; D006801:Humans; D008654:Mesothelioma / Q000737:chemistry* / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis*; D010997:Pleural Neoplasms / Q000737:chemistry* / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D012680:Sensitivity and Specificity; D025521:Tumor Suppressor Proteins / Q000032:analysis*; D043222:Ubiquitin Thiolesterase / Q000032:analysis*
chemical_list: C400134:BAP1 protein, human; D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D025521:Tumor Suppressor Proteins; D043222:Ubiquitin Thiolesterase
keywords: BAP1; Cytopathologie; Cytopathology; Malignant diffuse mesothelioma; Mésothéliome malin diffus; Pleura; Plèvre