title: Identification and functional characterization of fish-egg lectin in zebrafish.
abstract: Fish-egg lectins (FELs) are identified in several species of fishes, but their activity and mode of action remain largely unknown in early life stages. Here we showed that zebrafish FEL (zFEL) was a maternal factor, which was capable of interacting with Gram-negative and Gram-positive bacteria and enhancing the phagocytosis of the bacteria by macrophages. Interestingly, microinjection of purified native zFEL into the embryos (resulting in the increase of zFEL in the embryos) markedly promoted the resistance of the embryos to the pathogenic Aeromonas hydrophila. Taken together, zFEL appears a maternal immune-relevant molecule capable of defending the developing embryos/larvae from pathogenic attacks.
authors.affiliation: Laboratory for Evolution & Development, Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China; Department of Marine Biology, Ocean University of China, Qingdao 266003, China.
authors.forename: Yashuo
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Laboratory for Evolution & Development, Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China.
authors.forename: Lingzhen
authors.initials: L
authors.lastname: Bu
authors.affiliation: Laboratory for Evolution & Development, Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Yang
authors.affiliation: Laboratory for Evolution & Development, Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China; Department of Marine Biology, Ocean University of China, Qingdao 266003, China. Electronic address: hongyanli@ouc.edu.cn.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
authors.affiliation: Laboratory for Evolution & Development, Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China; Department of Marine Biology, Ocean University of China, Qingdao 266003, China. Electronic address: sczhang@ouc.edu.cn.
authors.forename: Shicui
authors.initials: S
authors.lastname: Zhang
mesh_terms: D016980:Aeromonas hydrophila / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000276:immunology; D029941:Fish Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D006090:Gram-Negative Bacteria / Q000502:physiology; D006094:Gram-Positive Bacteria / Q000502:physiology; D007113:Immunity, Innate / Q000187:drug effects / Q000276:immunology*; D037102:Lectins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015027:Zebrafish / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D037102:Lectins; D012333:RNA, Messenger
keywords: Fish-egg lectin; Maternal immunity; Opsonization; Phagocytosis; Zebrafish