title: Multiple Subsets of Brain Tumor Initiating Cells Coexist in Glioblastoma.
abstract: Brain tumor-initiating cells (BTICs) are self-renewing multipotent cells critical for tumor maintenance and growth. Using single-cell microfluidic profiling, we identified multiple subpopulations of BTICs coexisting in human glioblastoma, characterized by distinct surface marker expression and single-cell molecular profiles relating to divergent bulk tissue molecular subtypes. These data suggest BTIC subpopulation heterogeneity as an underlying source of intra-tumoral bulk tissue molecular heterogeneity, and will support future studies into BTIC subpopulation-specific therapies. Stem Cells 2016;34:1702-1707.
authors.affiliation: Department of Surgery, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Robert C
authors.initials: RC
authors.lastname: Rennert
authors.affiliation: Department of Neurosurgery, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Achal S
authors.initials: AS
authors.lastname: Achrol
authors.affiliation: Department of Surgery, Stanford University School of Medicine, Stanford, California, USA.|Stanford Center for Biomedical Informatics, Stanford University, Stanford, California, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Januszyk
authors.affiliation: Department of Neurosurgery, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Suzana A
authors.initials: SA
authors.lastname: Kahn
authors.affiliation: Stanford Center for Biomedical Informatics, Stanford University, Stanford, California, USA.
authors.forename: Tiffany T
authors.initials: TT
authors.lastname: Liu
authors.affiliation: Stanford Center for Biomedical Informatics, Stanford University, Stanford, California, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Stanford Center for Biomedical Informatics, Stanford University, Stanford, California, USA.
authors.forename: Debashis
authors.initials: D
authors.lastname: Sahoo
authors.affiliation: Department of Surgery, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Melanie
authors.initials: M
authors.lastname: Rodrigues
authors.affiliation: Department of Surgery, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Zeshaan N
authors.initials: ZN
authors.lastname: Maan
authors.affiliation: Department of Surgery, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Victor W
authors.initials: VW
authors.lastname: Wong
authors.affiliation: Department of Neurosurgery, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Samuel H
authors.initials: SH
authors.lastname: Cheshier
authors.affiliation: Department of Neurosurgery, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Steven D
authors.initials: SD
authors.lastname: Chang
authors.affiliation: Department of Neurosurgery, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Gary K
authors.initials: GK
authors.lastname: Steinberg
authors.affiliation: Department of Neurosurgery, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Griffith R
authors.initials: GR
authors.lastname: Harsh
authors.affiliation: Department of Surgery, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Geoffrey C
authors.initials: GC
authors.lastname: Gurtner
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D005909:Glioblastoma / Q000235:genetics / Q000473:pathology*; D006801:Humans; D014411:Neoplastic Stem Cells / Q000473:pathology*; D010641:Phenotype; D059010:Single-Cell Analysis; D014158:Transcription, Genetic
chemical_list: D014408:Biomarkers, Tumor
keywords: Brain tumor initiating cells; Glioblastoma; Microfluidics; Molecular heterogeneity