title: Comparison of the cryo-tolerance of vitrified gorgonian oocytes.
abstract: Coral reefs have been declining considerably in recent years because of changes to the environment and climate. The cryopreservation of coral gametes is an essential alternative method that yields immense success in preserving corals. This study focuses on developing vitrification techniques for Junceella fragilis and Ellisella robusta oocytes, and presents a comparison on the cryotolerance of their vitrified oocytes. The results revealed that these coral oocytes could be preserved for a longer period in equilibration solution 2 and vitrification solution (VS) 2 at 5 °C than at 26 °C. Oocyte viability decreased significantly when VS2 was used for >4 min at 26 °C compared with the control. Cryoprotectant tolerance was higher in E. robusta oocytes than in J. fragilis oocytes. However, E. robusta was determined to be more cryo-tolerant, with differences attributed to their habitats, thus making E. robusta is likely a superior candidate species for further study. The results of this study on the effects of coral cryopreservation provide a foundation for developing protocols further for the cryopreservation of the oocytes of gorgonian corals.
authors.affiliation: Department of Biotechnology, Mingdao University, 369 Wen-Hua Road, Peetow, ChangHua, 52345, Taiwan.|Department of Post Modern Agriculture, Mingdao University, 369 Wen-Hua Road, Peetow, Chang Hua, 52345, Taiwan.
authors.forename: Sujune
authors.initials: S
authors.lastname: Tsai
authors.affiliation: Institute of Marine Biology, National Dong Hwa University, 2 Houwan Road, Checheng, Pingtung, 944, Taiwan.
authors.forename: Vivian
authors.initials: V
authors.lastname: Yang
authors.affiliation: Institute of Marine Biology, National Dong Hwa University, 2 Houwan Road, Checheng, Pingtung, 944, Taiwan.|National museum of Marine Biology &Aquarium, 2 Houwan Road, Checheng, Pingtung, 944, Taiwan.
authors.forename: Chiahsin
authors.initials: C
authors.lastname: Lin
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D037421:Anthozoa / Q000254:growth & development / Q000502:physiology*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D017753:Ecosystem; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D013696:Temperature; D058989:Vitrification / Q000187:drug effects*
chemical_list: D003451:Cryoprotective Agents; D000255:Adenosine Triphosphate