title: Translocation frequency of double-stranded DNA through a solid-state nanopore.
abstract: Solid-state nanopores are single-molecule sensors that measure changes in ionic current as charged polymers such as DNA pass through. Here, we present comprehensive experiments on the length, voltage, and salt dependence of the frequency of double-stranded DNA translocations through conical quartz nanopores with mean opening diameter 15 nm. We observe an entropic barrier-limited, length-dependent translocation frequency at 4M LiCl salt concentration and a drift-dominated, length-independent translocation frequency at 1M KCl salt concentration. These observations are described by a unifying convection-diffusion equation, which includes the contribution of an entropic barrier for polymer entry.
authors.affiliation: Cavendish Laboratory, University of Cambridge, CB3 0HE, United Kingdom.
authors.forename: Nicholas A W
authors.initials: NA
authors.lastname: Bell
authors.affiliation: Cavendish Laboratory, University of Cambridge, CB3 0HE, United Kingdom.|Polymer Science and Engineering Department, University of Massachusetts, Amherst, Massachusetts 01003, USA.
authors.forename: Murugappan
authors.initials: M
authors.lastname: Muthukumar
authors.affiliation: Cavendish Laboratory, University of Cambridge, CB3 0HE, United Kingdom.
authors.forename: Ulrich F
authors.initials: UF
authors.lastname: Keyser
mesh_terms: D020029:Base Pairing; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D009068:Movement; D058608:Nanopores; D011189:Potassium Chloride / Q000737:chemistry
chemical_list: D011189:Potassium Chloride; D004247:DNA