title: [Effects of Ethanol Extracts of Phellinus lonicerinus on Hepatic Stellate Cells of Fibrosis Liver in Rats].
abstract: OBJECTIVE
  To study the effects of ethanol extracts of Phellinus lonicerinus on hepatic stellate cells of fibrosis liver in rats.
  
  
  METHODS
  The model rats of hepatic fibrosis were established by intraperitoneally injection of CCl₄ olive oil solution at the dose of 2 mL/kg. And then the model rats were administered orally ethanol extract of Phellinus lonicerinus with 1 g/(kg · d) and 0.5 g/( kg · d) for consecutive eight weeks. Serum transforming growth factor-β₁, (TGF-β₁), liver total antioxidant capacity (T-AOC), total superoxide dismutase( T-SOD) activity and the content of malondialdehyde ( MDA) were determined. The α-smooth muscle actin (α-SMA), matrix metalloproteinases-1 (MMP-1) and tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) were determined by immunohistochemical.
  
  
  RESULTS
  Compared to the model group, the ethanol extracts of Phellinus lonicerinus decreased the content of serum TGF-β₁ and MDA in liver tissue, as well as increased the activity of T-SOD and the level of T-AOC. Immunohistochemical showed that the ethanol extracts of Phellinus lonicerinus downregulated the expression of α-SMA in liver tissue and the expression of TIMP-1, as well as upregulated the expression of MMP-1.
  
  
  CONCLUSIONS
  The ethanol extracts of Phellinus lonicerinus has good antioxidative activity with application prospects in prevention of hepatic fibrosis. The mechanism may be related to inhibiting the activation of hepatic stellate cells induced by oxidative stress,reducing the TGF-β₁, and cytokines activating the hepatic stellate cells,and upregulating the expression of MMP-1 promoting the collagen degradation.
authors.forename: Jun-zhi
authors.initials: JZ
authors.lastname: Wang
authors.forename: Ling
authors.initials: L
authors.lastname: Wu
authors.forename: You-cheng
authors.initials: YC
authors.lastname: Luo
authors.forename: Hong-qi
authors.initials: HQ
authors.lastname: Zhang
authors.forename: Wen-feng
authors.initials: WF
authors.lastname: Huang
authors.forename: Xi-ming
authors.initials: XM
authors.lastname: Yan
authors.forename: Pan
authors.initials: P
authors.lastname: Zhang
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001487:Basidiomycota / Q000737:chemistry*; D001688:Biological Products / Q000494:pharmacology*; D002251:Carbon Tetrachloride; D055166:Hepatic Stellate Cells / Q000187:drug effects*; D008103:Liver Cirrhosis / Q000188:drug therapy*; D008315:Malondialdehyde / Q000378:metabolism; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D018384:Oxidative Stress; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000097:blood
chemical_list: D000199:Actins; D000975:Antioxidants; D001688:Biological Products; D002251:Carbon Tetrachloride; D008315:Malondialdehyde; D020781:Matrix Metalloproteinase 1; D013482:Superoxide Dismutase; D019715:Tissue Inhibitor of Metalloproteinase-1; D053773:Transforming Growth Factor beta1; C576839:TIMP1 protein, rat; C508962:Tgfb1 protein, rat; C576840:MMP1 protein, rat; C498862:Acta2 protein, rat