title: Enhanced bioconversion rate and released substrate inhibition in (R)-phenylephrine whole-cell bioconversion via partial acetone treatment.
abstract: An approach was developed to enhance the efficiency for the bioconversion of 1-(3-hydroxyphenyl)-2-(methyamino)-ethanone to (R)-phenylephrine. The strain Serratia marcescens N10612, giving the benefit of 99% enantiomeric excess in (R)-PE conversion, was used. The fermentation was devised to harvest cells with high hydrophobic prodigiosin content inside the cells. Then, the partial acetone extraction was applied to remove prodigiosin from the cells. The treatment was found to increase the cells conversion rate without loss of the cells NADPH redox system. When using 50% (v/v) acetone for 5min, the processed cells can give a specific conversion rate of 16.03μmol/h/g-cells. As compared the treated cells with cells under the basal medium, the maximum reaction rate (Vmax) increased from 6.69 to 10.27 (μmol/h/g-cells), the dissociation constant (Km) decreased from 0.236 to 0.167mM and the substrate inhibition constant (KSi) increased from 0.073 to 1.521mM. The 20-fold increase in substrate inhibition constant referred to a great release from the substrate inhibition for the use of S. marcescens N10612 in the bioconversion, which would greatly benefit the bioconversion to be industrialized.
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC.
authors.forename: Shu-Chen
authors.initials: SC
authors.lastname: Kan
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC.
authors.forename: Chi-Zong
authors.initials: CZ
authors.lastname: Zang
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC.
authors.forename: Chiung-Wen
authors.initials: CW
authors.lastname: Yeh
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC.
authors.forename: Wei-Feng
authors.initials: WF
authors.lastname: Chang
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC.
authors.forename: Chia-Chi
authors.initials: CC
authors.lastname: Lin
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC.
authors.forename: Tzu-Hsiang
authors.initials: TH
authors.lastname: Hung
authors.affiliation: Biotechnology Center, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC.
authors.forename: Chwen-Jen
authors.initials: CJ
authors.lastname: Shieh
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC. Electronic address: ycliu@dragon.nchu.edu.tw.
authors.forename: Yung-Chuan
authors.initials: YC
authors.lastname: Liu
mesh_terms: D000096:Acetone / Q000494:pharmacology; D055162:Biocatalysis; D001711:Biotransformation / Q000187:drug effects; D005285:Fermentation; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D010656:Phenylephrine / Q000737:chemistry / Q000378:metabolism*; D011353:Prodigiosin / Q000378:metabolism; D012706:Serratia marcescens / Q000187:drug effects / Q000378:metabolism; D013237:Stereoisomerism
chemical_list: D000096:Acetone; D010656:Phenylephrine; D009249:NADP; D011353:Prodigiosin
keywords: (R)-Phenylephrine; Bioconversion; Fermentation; Kinetics; Prodigiosin