title: Characterization and Application of BiLA, a Psychrophilic α-Amylase from Bifidobacterium longum.
abstract: In this study, a novel α-amylase was cloned from Bifidobacterium longum and named BiLA. The enzyme exhibited optimal activity at 20 °C and a pH value of 5.0. Kinetic analysis using various carbohydrate substrates revealed that BiLA had the highest k(cat/)K(m) value for amylose. Interestingly, analysis of the enzymatic reaction products demonstrated that BiLA specifically catalyzed the hydrolysis of oligosaccharides and starches up to G5 from the nonreducing ends. To determine whether BiLA can be used to generate slowly digestible starch (SDS), starch was treated with BiLA, and the kinetic parameters were analyzed using porcine pancreatic α-amylase (PPA) and amyloglucosidase (AMG). Compared to normal starch, BiLA-treated starch showed lower k(cat)/K(m) values with PPA and AMG, suggesting that BiLA is a potential candidate for the production of SDS.
authors.affiliation: Department of Food Science and Nutrition and Center for Aging and Health Care, Hallym University , Hallymdaehak-gil 1, Chuncheon, Gangwon-do 200-702, South Korea.
authors.forename: Hye-Won
authors.initials: HW
authors.lastname: Lee
authors.affiliation: Department of Food Science and Nutrition and Center for Aging and Health Care, Hallym University , Hallymdaehak-gil 1, Chuncheon, Gangwon-do 200-702, South Korea.
authors.forename: Hye-Yeon
authors.initials: HY
authors.lastname: Jeon
authors.affiliation: Department of Food Science and Nutrition and Center for Aging and Health Care, Hallym University , Hallymdaehak-gil 1, Chuncheon, Gangwon-do 200-702, South Korea.
authors.forename: Hye-Jeong
authors.initials: HJ
authors.lastname: Choi
authors.affiliation: Department of Food Science and Nutrition and Center for Aging and Health Care, Hallym University , Hallymdaehak-gil 1, Chuncheon, Gangwon-do 200-702, South Korea.
authors.forename: Na-Ri
authors.initials: NR
authors.lastname: Kim
authors.affiliation: Department of Food Science and Nutrition and Center for Aging and Health Care, Hallym University , Hallymdaehak-gil 1, Chuncheon, Gangwon-do 200-702, South Korea.
authors.forename: Woo-Jae
authors.initials: WJ
authors.lastname: Choung
authors.affiliation: Department of Food Science and Nutrition and Center for Aging and Health Care, Hallym University , Hallymdaehak-gil 1, Chuncheon, Gangwon-do 200-702, South Korea.
authors.forename: Ye-Seul
authors.initials: YS
authors.lastname: Koo
authors.affiliation: Department of Food Science and Nutrition and Center for Aging and Health Care, Hallym University , Hallymdaehak-gil 1, Chuncheon, Gangwon-do 200-702, South Korea.
authors.forename: Dam-Seul
authors.initials: DS
authors.lastname: Ko
authors.affiliation: Department of Marine Food Science and Technology, Gangneung-Wonju National University , 120 Gangneung Daehangno, Gangneung, Gangwon 210-702, South Korea.
authors.forename: SangGuan
authors.initials: S
authors.lastname: You
authors.affiliation: Department of Food Science and Nutrition and Center for Aging and Health Care, Hallym University , Hallymdaehak-gil 1, Chuncheon, Gangwon-do 200-702, South Korea.
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Shim
mesh_terms: D000688:Amylose / Q000032:analysis / Q000378:metabolism; D000818:Animals; D001644:Bifidobacterium / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D055292:Pancreatic alpha-Amylases / Q000378:metabolism; D013213:Starch / Q000737:chemistry; D013552:Swine; D000516:alpha-Amylases / Q000235:genetics / Q000378:metabolism*
chemical_list: D013213:Starch; D000688:Amylose; D055292:Pancreatic alpha-Amylases; D000516:alpha-Amylases; D005087:Glucan 1,4-alpha-Glucosidase
keywords: Bifidobacterium longum; resistant starch; slowly digestible starch; α-amylase