title: Biological in situ characterization of polymeric microbubble contrast agents.
abstract: Polymeric microbubbles (MBs) are gas filled particles composed of a thin stabilized polymer shell that have been recently developed as valid contrast agents for the combined use of ultrasonography (US), magnetic resonance imaging (MRI) and single photon emission computer tomography (SPECT) imaging. Due to their buoyancy, the commonly available approaches to study their behaviour in complex media are not easily applicable and their use in modern medicine requires such behaviour to be fully elucidated. Here we have used for the first time flow cytometry as a new high throughput approach that allows characterisation of the MB dispersion, prior to and after exposure in different biological media and we have additionally developed a method that allows characterisation of the strongly bound proteins adsorbed on the MBs, to fully predict their biological behaviour in biological milieu.
authors.affiliation: Centre for BioNano Interactions, School of Chemistry & Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: Sha
authors.initials: S
authors.lastname: Wan
authors.affiliation: Surflay Nanotec GmbH, Max-Planck-Strasse 3, 12489 Berlin, Germany.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Egri
authors.affiliation: Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor Vergata. Via della Ricerca Scientifica 00133 Rome, Italy.
authors.forename: Letizia
authors.initials: L
authors.lastname: Oddo
authors.affiliation: Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor Vergata. Via della Ricerca Scientifica 00133 Rome, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Cerroni
authors.affiliation: Surflay Nanotec GmbH, Max-Planck-Strasse 3, 12489 Berlin, Germany.
authors.forename: Lars
authors.initials: L
authors.lastname: Dähne
authors.affiliation: Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor Vergata. Via della Ricerca Scientifica 00133 Rome, Italy.
authors.forename: Gaio
authors.initials: G
authors.lastname: Paradossi
authors.affiliation: Centre for BioNano Interactions, School of Chemistry & Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland; Groningen Research Institute of Pharmacy, Division Pharmacokinetics, Toxicology and Targeting, University of Groningen, A. Deusinglaan 1, 9713 AV Groningen, The Netherlands.
authors.forename: Anna
authors.initials: A
authors.lastname: Salvati
authors.affiliation: School of Geography, Earth and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom.
authors.forename: Iseult
authors.initials: I
authors.lastname: Lynch
authors.affiliation: Centre for BioNano Interactions, School of Chemistry & Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland. Electronic address: kenneth.a.dawson@cbni.ucd.ie.
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Dawson
authors.affiliation: Centre for BioNano Interactions, School of Chemistry & Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland; Department of Pharmacy and Medical Chemistry, Royal College of Surgeons in Ireland, 123St. Stephen Green, Dublin 2, Ireland. Electronic address: marcomonopoli@rcsi.ie.
authors.forename: Marco P
authors.initials: MP
authors.lastname: Monopoli
mesh_terms: D000818:Animals; D055598:Chemical Phenomena; D003287:Contrast Media / Q000737:chemistry*; D006801:Humans; D045423:Microbubbles; D011108:Polymers / Q000737:chemistry*; D000066970:Protein Corona / Q000737:chemistry; D013552:Swine
chemical_list: D003287:Contrast Media; D011108:Polymers; D000066970:Protein Corona
keywords: Bionano interactions; Dysopsonin proteins; Flow cytometry; Microbubbles; Opsonin proteins; Protein corona