title: Actylise treatment does not influence nitric oxide metabolites serum level.
abstract: BACKGROUND
  Nitric oxide (NO) is synthesized by Nitric Oxide Synthases (NOS), the family of enzymes capable to conduct the conversion of Arginine (Arg) into the NO and Citrulline (Cit). Currently, only the administration of recombinant tissue plasminogen activator (rtPA) is recommended for acute ischemic stroke (AIS) treatment. To allow solubility of rtPA, Arg is added as a constituent of the drug. Our purpose was to check the effect of alteplase administration on NO metabolites concentration in the blood.
  
  
  METHODS
  Eighteen AIS patients were selected into the study. Nine of them received thrombolytic therapy (rtPA group). The serum samples were obtained at 3 time-points for rtPA group (time-point 0: 1st-4th hour of stroke; time-point 1: immediately after rtPA administration; time-point 3: on day 5-7 from stroke onset). Remaining patients (non-rtPA group) had blood collection at two time-points: time-point 1: 1st-10th hour of stroke and time-point 2: on day 5-7 of stroke. Arg and Cit were determined by the automated ion-exchange chromatography using Amino Acids Analyzer. NO serum level was indirectly evaluated with the usage of commercially available kits that measuring the nitrate/nitrite level.
  
  
  RESULTS
  Significant increase of Arg serum level was noticed at time-point 1, directly after the iv thrombolysis in comparison to non-rtPA group. However, the products of the reaction catalyzed by NOS (NO and Cit) did not rise after the thrombolysis.
  
  
  CONCLUSIONS
  Current study showed that Arg administration simultaneously with rtPA, as a constituent of Actylise, does not affect serum NO metabolites level.
authors.affiliation: Department of Medical Chemistry, Medical University of Lublin, Lublin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Horecka
authors.affiliation: Department of Medical Chemistry, Medical University of Lublin, Lublin, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Szpetnar
authors.affiliation: Department of Medical Chemistry, Medical University of Lublin, Lublin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Hordyjewska
authors.affiliation: Department of Neurosurgery and Neurotraumatology, Saint Edvige Provincial Hospital No 2 in Rzeszow, Rzeszów, Poland.
authors.forename: Daniel
authors.initials: D
authors.lastname: Babula
authors.affiliation: Department of Internal Medicine, The Holy Family Specialist Hospital in Rudna Mala, Głogów, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Gołąb
authors.affiliation: Department of Medical Chemistry, Medical University of Lublin, Lublin, Poland. Electronic address: kurzepa@onet.pl.
authors.forename: Jacek
authors.initials: J
authors.lastname: Kurzepa
mesh_terms: D000368:Aged; D001120:Arginine / Q000008:administration & dosage / Q000097:blood*; D002956:Citrulline / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D009569:Nitric Oxide / Q000097:blood*; D020521:Stroke / Q000097:blood / Q000188:drug therapy*; D010959:Tissue Plasminogen Activator / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D002956:Citrulline; D009569:Nitric Oxide; D001120:Arginine; D010959:Tissue Plasminogen Activator
keywords: Arginine; Citrulline; Ischemic stroke; Nitric oxide; Thrombolysis