title: Reprogramming of mouse amniotic fluid cells using a PiggyBac transposon system.
abstract: Induced pluripotent stem (iPS) cells are generated from mouse and human somatic cells by forced expression of defined transcription factors using different methods. Amniotic fluid (AF) cells are easy to obtain from routinely scheduled procedures for prenatal diagnosis and iPS cells have been generated from human AF. Here, we generated iPS cells from mouse AF cells, using a non-viral-based approach constituted by the PiggyBac (PB) transposon system. All iPS cell lines obtained exhibited characteristics of pluripotent cells, including the ability to differentiate toward derivatives of all three germ layers in vitro and in vivo.
authors.affiliation: Stem Cells and Regenerative Medicine Laboratory, Fondazione Istituto di Ricerca Pediatrica Città della Speranza, Padova, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Bertin
authors.affiliation: Stem Cells and Regenerative Medicine Laboratory, Fondazione Istituto di Ricerca Pediatrica Città della Speranza, Padova, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Piccoli
authors.affiliation: Stem Cells and Regenerative Medicine Laboratory, Fondazione Istituto di Ricerca Pediatrica Città della Speranza, Padova, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Franzin
authors.affiliation: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Nagy
authors.affiliation: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Mileikovsky
authors.affiliation: Stem Cells and Regenerative Medicine Section, DBC Programme UCL, Institute of Child Health, Great Ormond Street Hospital for Children, London, UK.
authors.forename: P
authors.initials: P
authors.lastname: De Coppi
authors.affiliation: Stem Cells and Regenerative Medicine Laboratory, Fondazione Istituto di Ricerca Pediatrica Città della Speranza, Padova, Italy. Electronic address: m.pozzobon@irpcds.org.
authors.forename: M
authors.initials: M
authors.lastname: Pozzobon
mesh_terms: D000653:Amniotic Fluid; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D065150:Cellular Reprogramming / Q000235:genetics* / Q000276:immunology*; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism*; D051379:Mice