title: Detection of intra-brain cytoplasmic 1 (BC1) long noncoding RNA using graphene oxide-fluorescence beacon detector.
abstract: Detection of cellular expression of long noncoding RNAs (lncRNAs) was elusive due to the ambiguity of exposure of their reactive sequences associated with their secondary/tertiary structures and dynamic binding of proteins around lncRNAs. Herein, we developed graphene-based detection techniques exploiting the quenching capability of graphene oxide (GO) flakes for fluorescent dye (FAM)-labeled single-stranded siRNAs and consequent un-quenching by their detachment from GO by matching lncRNAs. A brain cytoplasmic 1 (BC1) lncRNA expression was significantly decreased by a siRNA, siBC1-1. GO quenched the FAM-labeled siBC1-1 peptide nucleic acid (PNA) probe, and this quenching was recovered by BC1. While FAM-siBC1-1-PNA-GO complex transfected spontaneously mouse or human neural stem cells, fluorescence was recovered only in mouse cells having high BC1 expression. Fluorescent dye-labeled single-stranded RNA-GO probe could detect the reactive exposed nucleic acid sequence of a cytoplasmic lncRNA expressing in the cytoplasm, which strategy can be used as a detection method of lncRNA expression.
authors.affiliation: Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Mee Young
authors.initials: MY
authors.lastname: Kim
authors.affiliation: Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul, Korea.|Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, Korea.
authors.forename: Do Won
authors.initials: DW
authors.lastname: Hwang
authors.affiliation: The National Creative Research Initiative Center for Intelligent Hybrids, School of Chemical &Biological Engineering, The WCU Program of Chemical Convergence for Energy &Environment, Seoul National University, Seoul, Korea.
authors.forename: Fangyuan
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Yoori
authors.initials: Y
authors.lastname: Choi
authors.affiliation: Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Jung Woo
authors.initials: JW
authors.lastname: Byun
authors.affiliation: Genolution Inc., Asan Institute for Life Sciences, Korea.
authors.forename: Dongho
authors.initials: D
authors.lastname: Kim
authors.affiliation: Genolution Inc., Asan Institute for Life Sciences, Korea.
authors.forename: Jee-Eun
authors.initials: JE
authors.lastname: Kim
authors.affiliation: The National Creative Research Initiative Center for Intelligent Hybrids, School of Chemical &Biological Engineering, The WCU Program of Chemical Convergence for Energy &Environment, Seoul National University, Seoul, Korea.
authors.forename: Kookheon
authors.initials: K
authors.lastname: Char
authors.affiliation: Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul, Korea.|Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, Korea.
authors.forename: Dong Soo
authors.initials: DS
authors.lastname: Lee
mesh_terms: D000818:Animals; D001483:Base Sequence; D015374:Biosensing Techniques; D002460:Cell Line; D005456:Fluorescent Dyes / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D055029:Inverted Repeat Sequences; D051379:Mice; D010087:Oxides / Q000737:chemistry; D020733:RNA, Small Cytoplasmic / Q000235:genetics* / Q000378:metabolism; D013050:Spectrometry, Fluorescence
chemical_list: C117734:BC1 RNA; D005456:Fluorescent Dyes; D010087:Oxides; D020733:RNA, Small Cytoplasmic; D006108:Graphite