title: Assessment of Polygonum capitatum Buch.-Ham. ex D.Don by metabolomics based on gas chromatography with mass spectrometry.
abstract: Polygonum capitatum is widely used in southwest China. It has considerable therapeutic efficacy for urinary tract infections. P. capitatum contains multiple components and quality assessment can be achieved by means of metabolic fingerprinting. In this paper, a new strategy for P. capitatum quality determination was developed. Eleven batches of P. capitatum were collected from five geographical areas in China including a standard batch regulated by Good Agriculture Practice. Gas chromatography with mass spectrometry was used to generate fingerprints from triplicate extractions to each batch (n = 33). Hierarchical clustering analysis was applied to assess similarities among the ten batches to the standard batches. Orthogonal projection to latent structures discriminate analysis, cross-validated with permutation tests, was performed to investigate discriminating metabolites. Results demonstrated that the overall evaluation hierarchical clustering analysis clustered two batches with distance > 3. Orthogonal projection to latent structures discriminate analysis (R(2) Y (cum) = 0.997, Q(2) (cum) = 0.97, CV-ANOVA = 8.48 × 10(-11) ) indicated that several sugars contributed to batch classification. This method is a rational approach that can classify against a regulated plant standard and distinguishes samples from different origins or processing time in a holistic manner and metabolites driving any differences can be easily identified.
authors.affiliation: Institute of Pharmaceutical Science, Faculty of Life Sciences and Medicine, King's College London, London, UK.
authors.forename: Pei
authors.initials: P
authors.lastname: Han
authors.affiliation: Institute of Pharmaceutical Science, Faculty of Life Sciences and Medicine, King's College London, London, UK.|Provincial Key Laboratory of Pharmaceutics in Guizhou Province, School of Pharmacy, Guiyang Medical University, Guiyang, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Institute of Pharmaceutical Science, Faculty of Life Sciences and Medicine, King's College London, London, UK.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Hylands
authors.affiliation: Institute of Pharmaceutical Science, Faculty of Life Sciences and Medicine, King's College London, London, UK.
authors.forename: Cristina
authors.initials: C
authors.lastname: Legido-Quigley
mesh_terms: D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D008516:Medicine, Chinese Traditional; D055432:Metabolomics; D028502:Polygonum / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004365:Drugs, Chinese Herbal
keywords: Chemometrics; Fingerprinting; Metabolomics; Polygonum capitatum; Quality assessment