title: Expression Profiling of Developing Zebrafish Retinal Cells.
abstract: During retinal development, a variety of different types of neurons are produced. Understanding how each of these types of retinal nerve cells is generated is important from a developmental biology perspective. It is equally important if one is interested in how to regenerate cells after an injury or a disease. To gain more insight into how retinal neurons develop in the zebrafish, we performed single-cell mRNA profiling and in situ hybridizations (ISHs) on retinal sections and whole-mount zebrafish. Through the series of ISHs, designed and performed solely by undergraduate students in the laboratory, we were able to retrospectively identify our single-cell mRNA profiles as most likely coming from developing amacrine cells. Further analysis of these profiles will reveal genes that can be mutated using genome editing techniques. Together these studies increase our knowledge of the genes driving development of different cell types in the zebrafish retina.
authors.affiliation: Department of Genetics, Development and Cell Biology, Iowa State University , Ames, Iowa.
authors.forename: Madelyn
authors.initials: M
authors.lastname: Mullally
authors.affiliation: Department of Genetics, Development and Cell Biology, Iowa State University , Ames, Iowa.
authors.forename: Caitlin
authors.initials: C
authors.lastname: Albrecht
authors.affiliation: Department of Genetics, Development and Cell Biology, Iowa State University , Ames, Iowa.
authors.forename: Mary
authors.initials: M
authors.lastname: Horton
authors.affiliation: Department of Genetics, Development and Cell Biology, Iowa State University , Ames, Iowa.
authors.forename: Lauren A
authors.initials: LA
authors.lastname: Laboissonniere
authors.affiliation: Department of Genetics, Development and Cell Biology, Iowa State University , Ames, Iowa.
authors.forename: Jillian J
authors.initials: JJ
authors.lastname: Goetz
authors.affiliation: Department of Genetics, Development and Cell Biology, Iowa State University , Ames, Iowa.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Chowdhury
authors.affiliation: Department of Genetics, Development and Cell Biology, Iowa State University , Ames, Iowa.
authors.forename: Alicia
authors.initials: A
authors.lastname: Manning
authors.affiliation: Department of Genetics, Development and Cell Biology, Iowa State University , Ames, Iowa.
authors.forename: Andrea K
authors.initials: AK
authors.lastname: Wester
authors.affiliation: Department of Genetics, Development and Cell Biology, Iowa State University , Ames, Iowa.
authors.forename: Quinton
authors.initials: Q
authors.lastname: Bose
authors.affiliation: Department of Genetics, Development and Cell Biology, Iowa State University , Ames, Iowa.
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Trimarchi
mesh_terms: D025042:Amacrine Cells / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D012160:Retina / Q000254:growth & development* / Q000378:metabolism; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000378:metabolism*; D015027:Zebrafish / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism