title: The antimicrobial activity of ZnO nanoparticles against Vibrio cholerae: Variation in response depends on biotype.
abstract: The potency of zinc oxide nanoparticles (NPs), with a core size of ~7-10nm, to inhibit cholera disease was investigated by demonstrating the effect on two biotypes (classical and El Tor) of O1 serogroup of Vibrio cholerae-El Tor was more susceptible both in planktonic and in biofilm forms. Interaction with ZnO NP results in deformed cellular architecture. Increased fluidity and depolarization of membrane, and protein leakage further confirmed the damages inflicted on Vibrio by NP. NP was shown to produce reactive oxygen species (ROS) and induce DNA damage. These results suggest that the antibacterial mechanism of ZnO action is most likely due to generation of ROS and disruption of bacterial membrane. The antimicrobial efficacy of NP has been validated in animal model. The synergistic action of NP and antibiotic suggests an alternative for the treatment of cholera.
authors.affiliation: Department of Biochemistry, Bose Institute, P1/12 CIT Scheme VIIM, Kolkata, India.
authors.forename: Shamila
authors.initials: S
authors.lastname: Sarwar
authors.affiliation: Department of Biochemistry, Bose Institute, P1/12 CIT Scheme VIIM, Kolkata, India.
authors.forename: Soumyananda
authors.initials: S
authors.lastname: Chakraborti
authors.affiliation: Department of Biochemistry, Bose Institute, P1/12 CIT Scheme VIIM, Kolkata, India.
authors.forename: Supriyo
authors.initials: S
authors.lastname: Bera
authors.affiliation: Division of Molecular Pathophysiology, National Institute of Cholera and Enteric Diseases, Beliaghata, Kolkata, India.
authors.forename: Irshad Ali
authors.initials: IA
authors.lastname: Sheikh
authors.affiliation: Division of Molecular Pathophysiology, National Institute of Cholera and Enteric Diseases, Beliaghata, Kolkata, India.
authors.forename: Kazi Mirajul
authors.initials: KM
authors.lastname: Hoque
authors.affiliation: Department of Biochemistry, Bose Institute, P1/12 CIT Scheme VIIM, Kolkata, India. Electronic address: pinak@jcbose.ac.in.
authors.forename: Pinak
authors.initials: P
authors.lastname: Chakrabarti
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002771:Cholera / Q000188:drug therapy; D053758:Nanoparticles; D014734:Vibrio cholerae / Q000187:drug effects*; D015034:Zinc Oxide
chemical_list: D000890:Anti-Infective Agents; D015034:Zinc Oxide
keywords: Antibacterial activity; Biofilms; Membrane fluidity; Reactive oxygen species; Vibrio cholerae; ZnO nanoparticle