title: [Antagonistic effect of gingerols against TNF-α release, ROS overproduction and RIP3 expression increase induced by lectin from Pinellia ternata].
abstract: To explore the antagonistic effect of gingerols against the inflammation induced by lectin from Pinellia ternata. In this study, ELISA method was used to determine the effect of different extracts from gingerols on the release of inflammatory factor TNF-α from macrophages induced by lectin from P. ternata. The fluorescence probe was used to determine the effect of gingerols on the changes in ROS of macrophages induced by lectin from P. ternata. The western-blot method was applied to study the effect of gingerols on the increase in expression of cell receptor interacting protein RIP3 in macrophages induced by lectin from P. ternata. The scanning electron microscope (SEM) was used to study the effect of gingerols on morphological changes in macrophages induced by lectin from P. ternata. According to the results, gingerols can significantly inhibit the release of inflammatory factor from macrophages induced by lectin from P. ternata, ROS overproduction and increase in RIP3 expression. SEM results showed that gingerols can inhibit the cytomorphosis and necrocytosis induced by lectin from P. ternata. Fresh ginger's detoxication may be related to gingerols' effects in inhibiing release of inflammatory factor, ROS overproduction and increase in RIP3 expression caused by macrophages induced by lectin from P. ternata, which are mainly inflammatory development.
authors.forename: Hong-li
authors.initials: HL
authors.lastname: Yu
authors.forename: Shan-hu
authors.initials: SH
authors.lastname: Mao
authors.forename: Teng-fei
authors.initials: TF
authors.lastname: Zhao
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.forename: Yao-zong
authors.initials: YZ
authors.lastname: Pan
authors.forename: Chen-yan
authors.initials: CY
authors.lastname: Shu
mesh_terms: D000818:Animals; D002396:Catechols / Q000494:pharmacology*; D002478:Cells, Cultured; D004336:Drug Antagonism; D005233:Fatty Alcohols / Q000494:pharmacology*; D020939:Zingiber officinale / Q000737:chemistry*; D037102:Lectins / Q000633:toxicity*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D030021:Pinellia / Q000737:chemistry / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D053422:Receptor-Interacting Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D002396:Catechols; D005233:Fatty Alcohols; D037102:Lectins; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; C007845:gingerol; D053422:Receptor-Interacting Protein Serine-Threonine Kinases; C485615:Ripk3 protein, mouse