title: Dissection of Individual Prostate Lobes in Mouse Models of Prostate Cancer to Obtain High Quality RNA.
abstract: Genetically engineered mouse models of prostate cancer allow for study of disease progression from localized tumor formation through distal metastasis. The anatomy of the mouse prostate differs dramatically from the human prostate, being composed of four lobe pairs (anterior, dorsal, lateral, and ventral), making the identification and dissection technically challenging. Although the entire murine prostate and surrounding tissue, including urethra, bladder, seminal vesicles, and associated adipose tissue, can be quickly dissected for en bloc analysis, it is necessary to isolate individual prostate lobes for gene expression studies elucidating the molecular mechanisms of prostate cancer. The procedure as described here includes full color images, allowing the researcher to appreciate the unique prostate morphology and tissue manipulation required to harvest individual prostate lobes. Along with removing all extraneous tissue, the procedure allows for direct comparison of the different prostate lobes by established downstream techniques. Importantly, high quality RNA required for next-generation gene expression analysis can only consistently be obtained from ventral and lateral lobes. Finally, preclinical studies using prostate targeted therapies can be monitored specifically in individual prostate lobes for histological and gene expression studies. J. Cell. Physiol. 232: 14-18, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Biochemistry, University of Vermont Cancer Center, University of Vermont College of Medicine, Burlington, Vermont.
authors.forename: Areg
authors.initials: A
authors.lastname: Zingiryan
authors.affiliation: Department of Biochemistry, University of Vermont Cancer Center, University of Vermont College of Medicine, Burlington, Vermont. nicholas.farina@med.uvm.edu.
authors.forename: Nicholas H
authors.initials: NH
authors.lastname: Farina
authors.affiliation: Department of Biochemistry, University of Vermont Cancer Center, University of Vermont College of Medicine, Burlington, Vermont.
authors.forename: Kristiaan H
authors.initials: KH
authors.lastname: Finstad
authors.affiliation: Department of Biochemistry, University of Vermont Cancer Center, University of Vermont College of Medicine, Burlington, Vermont.
authors.forename: Janet L
authors.initials: JL
authors.lastname: Stein
authors.affiliation: Department of Biochemistry, University of Vermont Cancer Center, University of Vermont College of Medicine, Burlington, Vermont.
authors.forename: Jane B
authors.initials: JB
authors.lastname: Lian
authors.affiliation: Department of Biochemistry, University of Vermont Cancer Center, University of Vermont College of Medicine, Burlington, Vermont.
authors.forename: Gary S
authors.initials: GS
authors.lastname: Stein
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D008297:Male; D051379:Mice; D009919:Orchiectomy / Q000379:methods; D011467:Prostate / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D012313:RNA / Q000378:metabolism*
chemical_list: D012313:RNA