title: Paraoxonase 1 and 2 gene variants and the ischemic stroke risk in Gran Canaria population: an association study and meta-analysis.
abstract: OBJECTIVE
  The present study aims to evaluate the relationship between rs662 (Gln(Q)192Arg(R)) and rs854560 (L55M) and the rs7493 (S311C) in the paraoxonase genes and ischemic stroke (IS) in the population of Gran Canaria (Canary Islands). The association with stroke was also evaluated using systematic review and meta-analysis.
  
  
  METHODS
  A total of 129 IS patients and 176 age and gender matched controls were enrolled. For meta-analysis, eligible studies were identified through search in public databases.
  
  
  RESULTS
  In multivariate regression analysis only the PON2 S311C variant showed to be an independent predictor of IS (OR = 0.093, 95% CI: 0.014-0.627). Overall, no significant association was found between L55M and IS when all studies were pooled nor by subgroup analysis by ethnicity. Gln192Arg showed a modest risk for IS in the global and in Asian population but with high heterogeneity among studies. A modest risk under a dominant inheritance model was found for the S311C variant with an overall random effect OR of 1.004 (95% CI: 1.00-1.35). There was strong evidence of heterogeneity among studies (  p = 0.0097, I2= 25.35%) which did not disappear after stratification by ethnicity.
  
  
  CONCLUSIONS
  The overall analysis shows a significant contribution of the rs662 variant to IS risk. We found that the CC genotype of the PON2 S311C polymorphism is a risk factor for IS. Results of the meta-analysis partially support this conclusion.
authors.affiliation: a Unidad de Investigación, Hospital Universitario de Gran Canaria Doctor Negrín , Gran Canaria , Spain.|c Universidad de Las Palmas de Gran Canaria (ULPGC) , Gran Canaria , Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Rodríguez-Esparragón
authors.affiliation: a Unidad de Investigación, Hospital Universitario de Gran Canaria Doctor Negrín , Gran Canaria , Spain.|b Servicio de Neurología, Hospital Universitario de Gran Canaria Doctor Negrín , Gran Canaria , Spain.|c Universidad de Las Palmas de Gran Canaria (ULPGC) , Gran Canaria , Spain.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: López-Fernández
authors.affiliation: a Unidad de Investigación, Hospital Universitario de Gran Canaria Doctor Negrín , Gran Canaria , Spain.
authors.forename: Nisa
authors.initials: N
authors.lastname: Buset-Ríos
authors.affiliation: a Unidad de Investigación, Hospital Universitario de Gran Canaria Doctor Negrín , Gran Canaria , Spain.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: García-Bello
authors.affiliation: a Unidad de Investigación, Hospital Universitario de Gran Canaria Doctor Negrín , Gran Canaria , Spain.
authors.forename: Erika
authors.initials: E
authors.lastname: Hernández-Velazquez
authors.affiliation: a Unidad de Investigación, Hospital Universitario de Gran Canaria Doctor Negrín , Gran Canaria , Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Cappiello
authors.affiliation: a Unidad de Investigación, Hospital Universitario de Gran Canaria Doctor Negrín , Gran Canaria , Spain.|b Servicio de Neurología, Hospital Universitario de Gran Canaria Doctor Negrín , Gran Canaria , Spain.|c Universidad de Las Palmas de Gran Canaria (ULPGC) , Gran Canaria , Spain.
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Rodríguez-Pérez
mesh_terms: D000110:Acetylcholinesterase / Q000097:blood; D000328:Adult; D000368:Aged; D043303:Aryldialkylphosphatase / Q000235:genetics*; D002545:Brain Ischemia / Q000150:complications; D018672:Community Health Planning; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011446:Prospective Studies; D013030:Spain / Q000453:epidemiology; D018709:Statistics, Nonparametric; D020521:Stroke / Q000097:blood / Q000201:enzymology* / Q000209:etiology / Q000235:genetics*
chemical_list: D000110:Acetylcholinesterase; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human; C468411:PON2 protein, human
keywords: genetics; paraoxonase; polymorphisms; stroke