title: Conditional Control of CRISPR/Cas9 Function.
abstract: The recently discovered CRISPR/Cas9 endonuclease system, comprised of a guide RNA for the recognition of a DNA target and the Cas9 nuclease protein for binding and processing the target, has been extensively studied and has been widely applied in genome editing, synthetic biology, and transcriptional modulation in cells and animals. Toward more precise genomic modification and further expansion of the CRISPR/Cas9 system as a spatiotemporally controlled gene regulatory system, several approaches of conditional activation of Cas9 function using small molecules and light have recently been developed. These methods have led to improvements in the genome editing specificity of the CRISPR/Cas9 system and enabled its activation with temporal and spatial precision.
authors.affiliation: Department of Chemistry, University of Pittsburgh, Pittsburgh, PA, 15260, USA.
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Department of Chemistry, University of Pittsburgh, Pittsburgh, PA, 15260, USA. deiters@pitt.edu.
authors.forename: Alexander
authors.initials: A
authors.lastname: Deiters
mesh_terms: D000818:Animals; D064113:CRISPR-Cas Systems; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D004720:Endonucleases / Q000235:genetics / Q000378:metabolism*; D000072669:Gene Editing / Q000379:methods*; D018390:Gene Targeting / Q000379:methods*; D006801:Humans; D008027:Light; D000094704:RNA, Guide, CRISPR-Cas Systems / Q000235:genetics / Q000378:metabolism; D054852:Small Molecule Libraries / Q000378:metabolism
chemical_list: D000094704:RNA, Guide, CRISPR-Cas Systems; D054852:Small Molecule Libraries; D004720:Endonucleases
keywords: CRISPR; Cas9; conditional control; genomic editing; light; small molecules