title: Adaptive engineering of a hyperthermophilic archaeon on CO and discovering the underlying mechanism by multi-omics analysis.
abstract: The hyperthermophilic archaeon Thermococcus onnurineus NA1 can grow and produce H2 on carbon monoxide (CO) and its H2 production rates have been improved through metabolic engineering. In this study, we applied adaptive evolution to enhance H2 productivity. After over 150 serial transfers onto CO medium, cell density, CO consumption rate and H2 production rate increased. The underlying mechanism for those physiological changes could be explained by using multi-omics approaches including genomic, transcriptomic and epigenomic analyses. A putative transcriptional regulator was newly identified to regulate the expression levels of genes related to CO oxidation. Transcriptome analysis revealed significant changes in the transcript levels of genes belonging to the categories of transcription, translation and energy metabolism. Our study presents the first genome-scale methylation pattern of hyperthermophilic archaea. Adaptive evolution led to highly enhanced H2 productivity at high CO flow rates using synthesis gas produced from coal gasification.
authors.affiliation: Korea Institute of Ocean Science and Technology, Ansan, Republic of Korea.|Department of Marine Biotechnology, Korea University of Science and Technology, Daejeon, Republic of Korea.
authors.forename: Seong Hyuk
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Korea Institute of Energy Research, Daejeon, Republic of Korea.
authors.forename: Min-Sik
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Korea Institute of Ocean Science and Technology, Ansan, Republic of Korea.
authors.forename: Jae-Hak
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Korea Institute of Ocean Science and Technology, Ansan, Republic of Korea.|Department of Marine Biotechnology, Korea University of Science and Technology, Daejeon, Republic of Korea.
authors.forename: Tae Wan
authors.initials: TW
authors.lastname: Kim
authors.affiliation: Korea Institute of Ocean Science and Technology, Ansan, Republic of Korea.
authors.forename: Seung Seob
authors.initials: SS
authors.lastname: Bae
authors.affiliation: Korea Institute of Ocean Science and Technology, Ansan, Republic of Korea.
authors.forename: Sung-Mok
authors.initials: SM
authors.lastname: Lee
authors.affiliation: Korea Institute of Ocean Science and Technology, Ansan, Republic of Korea.|Department of Marine Biotechnology, Korea University of Science and Technology, Daejeon, Republic of Korea.
authors.forename: Hae Chang
authors.initials: HC
authors.lastname: Jung
authors.affiliation: Korea Institute of Ocean Science and Technology, Ansan, Republic of Korea.
authors.forename: Tae-Jun
authors.initials: TJ
authors.lastname: Yang
authors.affiliation: Korea Institute of Ocean Science and Technology, Ansan, Republic of Korea.
authors.forename: Ae Ran
authors.initials: AR
authors.lastname: Choi
authors.affiliation: Chunlab, Inc., Seoul, Republic of Korea.
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Cho
authors.affiliation: Korea Institute of Ocean Science and Technology, Ansan, Republic of Korea.|Department of Marine Biotechnology, Korea University of Science and Technology, Daejeon, Republic of Korea.
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Korea Institute of Ocean Science and Technology, Ansan, Republic of Korea.|Department of Marine Biotechnology, Korea University of Science and Technology, Daejeon, Republic of Korea.
authors.forename: Kae Kyoung
authors.initials: KK
authors.lastname: Kwon
authors.affiliation: Korea Institute of Ocean Science and Technology, Ansan, Republic of Korea.|Department of Marine Biotechnology, Korea University of Science and Technology, Daejeon, Republic of Korea.
authors.forename: Hyun Sook
authors.initials: HS
authors.lastname: Lee
authors.affiliation: Korea Institute of Ocean Science and Technology, Ansan, Republic of Korea.|Department of Marine Biotechnology, Korea University of Science and Technology, Daejeon, Republic of Korea.
authors.forename: Sung Gyun
authors.initials: SG
authors.lastname: Kang
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D002248:Carbon Monoxide / Q000378:metabolism*; D019175:DNA Methylation; D004734:Energy Metabolism / Q000235:genetics; D057890:Epigenomics / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods*; D019766:Genes, Archaeal / Q000235:genetics; D020745:Genome, Archaeal / Q000235:genetics; D023281:Genomics / Q000379:methods*; D006358:Hot Temperature; D006859:Hydrogen / Q000378:metabolism; D060847:Metabolic Engineering / Q000379:methods; D009154:Mutation; D010084:Oxidation-Reduction; D019712:Thermococcus / Q000235:genetics* / Q000378:metabolism
chemical_list: D002248:Carbon Monoxide; D006859:Hydrogen