title: More for less: Improving the biomass yield of a pear cell suspension culture by design of experiments.
abstract: Plant cell suspension cultures are widely used for the production of recombinant proteins and secondary metabolites. One of the most important steps during process development is the optimization of yields by testing different cultivation parameters, including the components of the growth medium. However, we have shown that the biomass yield of a cell suspension culture derived from the pear cultivar Pyrus communis cv. Champagner Bratbirne can be significantly improved solely by varying the temperature, inoculum density, illumination, and incubation time. In contrast to medium optimization, these simple physical factors are easily controlled and varied, thereby reducing the effort required. Using an experimental design approach, we improved the biomass yield from 146 g fresh weight (FW)/L to 407 g FW/L in only 5 weeks, simultaneously reducing the costs of goods sold per kg biomass from € 125 to € 45. Our simple approach therefore offers a rapid, efficient and economical process for the optimization of plant cell suspension cultures.
authors.affiliation: Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstraße 6, 52074 Aachen, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Rasche
authors.affiliation: Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstraße 6, 52074 Aachen, Germany.
authors.forename: Denise
authors.initials: D
authors.lastname: Herwartz
authors.affiliation: Institute for Molecular Biotechnology, Worringerweg 1, RWTH Aachen University, 52074 Aachen, Germany.
authors.forename: Flora
authors.initials: F
authors.lastname: Schuster
authors.affiliation: Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstraße 6, 52074 Aachen, Germany.
authors.forename: Natalia
authors.initials: N
authors.lastname: Jablonka
authors.affiliation: Dr. Babor GmbH &Co. KG, Neuenhofstraße 180, 52078 Aachen, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Weber
authors.affiliation: Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstraße 6, 52074 Aachen, Germany.|Institute for Molecular Biotechnology, Worringerweg 1, RWTH Aachen University, 52074 Aachen, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Fischer
authors.affiliation: Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstraße 6, 52074 Aachen, Germany.|Justus-Liebig University Giessen, Institute for Phytopathology and Applied Zoology, Phytopathology Department, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Schillberg
mesh_terms: D018533:Biomass; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D003470:Culture Media; D031989:Pyrus / Q000166:cytology* / Q000254:growth & development*
chemical_list: D003470:Culture Media