title: Studies of RNA Sequence and Structure Using Nanopores.
abstract: Nanopores are powerful single-molecule sensors with nanometer scale dimensions suitable for detection, quantification, and characterization of nucleic acids and proteins. Beyond sequencing applications, both biological and solid-state nanopores hold great promise as tools for studying the biophysical properties of RNA. In this review, we highlight selected landmark nanopore studies with regards to RNA sequencing, microRNA detection, RNA/ligand interactions, and RNA structural/conformational analysis.
authors.affiliation: Department of Physics, Northeastern University, Boston, Massachusetts, USA.
authors.forename: Robert Y
authors.initials: RY
authors.lastname: Henley
authors.affiliation: Department of Physics, Northeastern University, Boston, Massachusetts, USA.
authors.forename: Spencer
authors.initials: S
authors.lastname: Carson
authors.affiliation: Department of Physics, Northeastern University, Boston, Massachusetts, USA; Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA. Electronic address: wanunu@neu.edu.
authors.forename: Meni
authors.initials: M
authors.lastname: Wanunu
mesh_terms: D000818:Animals; D001483:Base Sequence; D006801:Humans; D058608:Nanopores; D009690:Nucleic Acid Conformation; D019095:Point-of-Care Systems; D012313:RNA / Q000737:chemistry* / Q000235:genetics*; D017423:Sequence Analysis, RNA
chemical_list: D012313:RNA
keywords: RNA; RNA conformation; RNA sequencing; RNA–protein interaction; drug screening assay; microRNA; nanopore; single-molecule