title: Decreased Expression of Tumor-suppressor Gene LKB1 Correlates with Poor Prognosis in Human Gastric Cancer.
abstract: OBJECTIVE
  The present report describes the correlation of liver kinase B1 (LKB1) expression with tumorigenesis and prognosis in gastric cancer.
  
  
  METHODS
  LKB1 mRNA and protein expression was detected in gastric-cancer cell lines and patient specimens. Patients were followed-up and clinico-pathological parameters and overall survival (OS) were evaluated.
  
  
  RESULTS
  The expression of LKB1 mRNA and protein was lower in gastric-cancer cell lines and tumor tissues compared to normal gastric cells (p<0.05) and tissues (p<0.001). Decreased expression of LKB1 mRNA and protein in patients with gastric cancer was significantly inversely related to TNM stage, T-stage (depth of invasion), lymph-node metastasis and vascular invasion (p<0.05). Patients showing high LKB1 mRNA and high LKB1 protein expression had a significantly longer OS and better 5-year survival rate than those with low mRNA expression (61.3 months vs. 56.1 months and 75% vs. 58.7%, p<0.05, respectively) and low protein expression (64.8 months vs. 55.7 months and 72.9% vs. 64.5%, p<0.05, respectively). Multivariate Cox regression analysis indicated that both LKB1 mRNA and protein expression in gastric cancer were independent prognostic factors for OS.
  
  
  CONCLUSIONS
  Patients with gastric cancer with decreased expression of LKB1 have a poor prognosis with a lower survival rate.
authors.affiliation: Department of Oncology, Affiliated Cancer Hospital, Nanjing Medical University, Nanjing, Jiangsu, P.R. China Department of Oncology, Wuxi No.4 Hospital, Affiliated to Jiangnan University, Nanjing, Jiangsu, P.R. China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Oncology, Affiliated Cancer Hospital, Nanjing Medical University, Nanjing, Jiangsu, P.R. China.
authors.forename: Binxun
authors.initials: B
authors.lastname: Ling
authors.affiliation: Department of Pathology, Affiliated Cancer Hospital, Nanjing Medical University, Nanjing, Jiangsu, P.R. China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Xu
authors.affiliation: Experimental Center of Clinical Oncology, Affiliated Cancer Hospital, Nanjing Medical University, Nanjing, Jiangsu, P.R. China.
authors.forename: Rong
authors.initials: R
authors.lastname: Ma
authors.affiliation: Department of General Surgery, Affiliated Cancer Hospital, Nanjing Medical University, Nanjing, Jiangsu, P.R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: Origin Biosciences Inc., Nanjing, Jiangsu, P.R. China.
authors.forename: Xinghua
authors.initials: X
authors.lastname: Cao
authors.affiliation: Origin Biosciences Inc., Nanjing, Jiangsu, P.R. China.
authors.forename: Wang
authors.initials: W
authors.lastname: Ling
authors.affiliation: Origin Biosciences Inc., Nanjing, Jiangsu, P.R. China AntiCancer Inc., San Diego, CA, U.S.A.
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Yang
authors.affiliation: AntiCancer Inc., San Diego, CA, U.S.A. Department of Surgery, UCSD, San Diego, CA, U.S.A. lujw@medmail.com.cn all@anticancer.com.n.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
authors.affiliation: Department of Oncology, Affiliated Cancer Hospital, Nanjing Medical University, Nanjing, Jiangsu, P.R. China lujw@medmail.com.cn all@anticancer.com.n.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Lu
mesh_terms: D000091162:AMP-Activated Protein Kinase Kinases; D045744:Cell Line, Tumor; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D009367:Neoplasm Staging; D011379:Prognosis; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism*; D012189:Retrospective Studies; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D016019:Survival Analysis
chemical_list: D017346:Protein Serine-Threonine Kinases; C109790:STK11 protein, human; D000091162:AMP-Activated Protein Kinase Kinases
keywords: LKB1 expression; prognosis and gastric cancer; survival