title: Polymorphisms of Selected DNA Repair Genes and Lung Cancer in Chromium Exposure.
abstract: Chromium is a well-known mutagen and carcinogen involved in lung cancer development. DNA repair genes play an important role in the elimination of genetic changes caused by chromium exposure. In the present study, we investigated the polymorphisms of the following DNA repair genes: XRCC3, participating in the homologous recombination repair, and hMLH1 and hMSH2, functioning in the mismatch repair. We focused on the risk the polymorphisms present in the development of lung cancer regarding the exposure to chromium. We analyzed 106 individuals; 45 patients exposed to chromium with diagnosed lung cancer and 61 healthy controls. Genotypes were determined by a PCR-RFLP method. We unravelled a potential for increased risk of lung cancer development in the hMLH1 (rs1800734) AA genotype in the recessive model. In conclusion, gene polymorphisms in the DNA repair genes underscores the risk of lung cancer development in chromium exposed individuals.
authors.affiliation: Division of Molecular Medicine, Biomedical Center Martin, Comenius University in Bratislava, 4/C Mala Hora St, 03601, Martin, Slovakia.|Department of Medical Biology, Comenius University in Bratislava, 4 Mala Hora St, 03601, Martin, Slovakia.
authors.forename: E
authors.initials: E
authors.lastname: Halasova
authors.affiliation: Department of Medical Biochemistry, Comenius University in Bratislava, 4/D Mala Hora St, 03601, Martin, Slovakia. matakova@jfmed.uniba.sk.
authors.forename: T
authors.initials: T
authors.lastname: Matakova
authors.affiliation: Department of Clinical Biochemistry, University Hospital Martin, 2 Kollárova St, 03601, Martin, Slovakia.
authors.forename: M
authors.initials: M
authors.lastname: Skerenova
authors.affiliation: Division of Molecular Medicine, Biomedical Center Martin, Comenius University in Bratislava, 4/C Mala Hora St, 03601, Martin, Slovakia.
authors.forename: M
authors.initials: M
authors.lastname: Krutakova
authors.affiliation: Division of Molecular Medicine, Biomedical Center Martin, Comenius University in Bratislava, 4/C Mala Hora St, 03601, Martin, Slovakia.
authors.forename: P
authors.initials: P
authors.lastname: Slovakova
authors.affiliation: Clinic of Thoracic Surgery and University Hospital Martin, Comenius University in Bratislava, 2 Kollárova St, 03601, Martin, Slovakia.
authors.forename: A
authors.initials: A
authors.lastname: Dzian
authors.affiliation: Clinic of Paediatrics, Central Military Hospital, 21 Generala Milosa Vesela St, 03426, Ruzomberok, Slovakia.
authors.forename: S
authors.initials: S
authors.lastname: Javorkova
authors.affiliation: Department of Medical Biology, Comenius University in Bratislava, 4 Mala Hora St, 03601, Martin, Slovakia.
authors.forename: M
authors.initials: M
authors.lastname: Pec
authors.affiliation: Department of Medical Biology, Comenius University in Bratislava, 4 Mala Hora St, 03601, Martin, Slovakia.
authors.forename: K
authors.initials: K
authors.lastname: Kypusova
authors.affiliation: Clinic of Thoracic Surgery and University Hospital Martin, Comenius University in Bratislava, 2 Kollárova St, 03601, Martin, Slovakia.
authors.forename: J
authors.initials: J
authors.lastname: Hamzik
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D002857:Chromium / Q000009:adverse effects*; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008175:Lung Neoplasms / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D000070957:MutL Protein Homolog 1 / Q000235:genetics*; D051718:MutS Homolog 2 Protein / Q000235:genetics*; D009367:Neoplasm Staging; D016273:Occupational Exposure / Q000009:adverse effects*; D011110:Polymorphism, Genetic / Q000235:genetics*; D011379:Prognosis; D012307:Risk Factors; D015996:Survival Rate
chemical_list: D004268:DNA-Binding Proteins; C501486:MLH1 protein, human; C110901:X-ray repair cross complementing protein 3; D002857:Chromium; C497172:MSH2 protein, human; D000070957:MutL Protein Homolog 1; D051718:MutS Homolog 2 Protein
keywords: Carcinogens; Chromium exposure; DNA repair; Genes; Genotype; Lung cancer; Polymorphism