title: DNA Sequential Logic Gate Using Two-Ring DNA.
abstract: Sequential DNA detection is a fundamental issue for elucidating the interactive relationships among complex gene systems. Here, a sequential logic DNA gate was achieved by utilizing the two-ring DNA structure, with the ability to recognize "before" and "after" triggering sequences of DNA signals. By taking advantage of a "loop-open" mechanism, separations of two-ring DNAs were controlled. Three triggering pathways with different sequential DNA treatments were distinguished by comparing fluorescent outputs. Programmed nanoparticle arrangement guided by "interlocked" two-ring DNA was also constructed to demonstrate the achievement of designed nanostrucutres. Such sequential logic DNA operation may guide future molecular sensors to monitor more complex gene network in biological systems.
authors.affiliation: Institute of Software, School of Electronics Engineering and Computer Science, Key Laboratory of High Confidence Software Technologies, Ministry of Education, Peking University , Beijing 100871, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Life Science, Shannxi Normal University , Xi'an 710062, China.
authors.forename: Linjing
authors.initials: L
authors.lastname: Shen
authors.affiliation: School of Control and Computer Engineering, North China Electric Power University , Beijing 102206, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Liang
authors.affiliation: College of Life Science, Shannxi Normal University , Xi'an 710062, China.
authors.forename: Yafei
authors.initials: Y
authors.lastname: Dong
authors.affiliation: School of Control and Computer Engineering, North China Electric Power University , Beijing 102206, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Institute of Software, School of Electronics Engineering and Computer Science, Key Laboratory of High Confidence Software Technologies, Ministry of Education, Peking University , Beijing 100871, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
mesh_terms: D039301:Computers, Molecular; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D053263:Gene Regulatory Networks / Q000235:genetics*; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D009690:Nucleic Acid Conformation
chemical_list: D006046:Gold; D004247:DNA
keywords: DNA logic gate; gold nanoparticle; interlocked structure; sequential detection; two-ring DNA