title: Comparative microRNA profiling in relation to urinary albumin excretion in newly diagnosed hypertensive patients.
abstract: Microalbuminuria is an established early marker of endothelial dysfunction and damage. MicroRNAs (miRNAs) are emerging as essential modulators of cardiovascular physiology and disease. In the present study, we sought an association between the differential expression of related miRNAs in the peripheral blood mononuclear cells of untreated patients with newly diagnosed essential hypertension and the levels of urinary albumin excretion. We assessed the expression of the miRNAs miRNA-1, miRNA-133a, miRNA-26b, miRNA-208b, miRNA-499 and miRNA-21 in consecutive subjects with untreated newly diagnosed essential hypertension (aged 62.5±9.7 years) and with no indications of other organic heart disease. MiRNA expression levels in peripheral blood mononuclear cells were quantified by real-time reverse transcription-polymerase chain reaction. The prevalence of microalbuminuria was 9.8%. miRNA-208b and miRNA-133a were independently correlated with 24-h urinary albumin excretion. More specifically, a strong association was found between the gene expression levels of miRNA-208b in our patients' peripheral blood cells and urinary albumin (r=0.72, P<0.001). A similar association was found for miRNA-133a (r=0.372, P<0.001). In conclusion, miRNA-208b and miRNA-133a show distinct profiling in peripheral blood cells isolated from untreated patients with recently diagnosed essential hypertension. Their gene expression levels reveal a strong correlation with urinary albumin excretion levels. Our findings provide new perspectives on the development of a new generation of biomarkers for the better monitoring of end-organ damage in hypertension.
authors.affiliation: Department of Cardiology, Heraklion University Hospital, Heraklion, Greece.
authors.forename: F I
authors.initials: FI
authors.lastname: Parthenakis
authors.affiliation: Department of Cardiology, Heraklion University Hospital, Heraklion, Greece.
authors.forename: M E
authors.initials: ME
authors.lastname: Marketou
authors.affiliation: Molecular Cardiology Laboratory, School of Medicine, University of Crete, Heraklion, Greece.
authors.forename: J E
authors.initials: JE
authors.lastname: Kontaraki
authors.affiliation: Department of Cardiology, Heraklion University Hospital, Heraklion, Greece.
authors.forename: F
authors.initials: F
authors.lastname: Maragoudakis
authors.affiliation: Department of Cardiology, Heraklion University Hospital, Heraklion, Greece.
authors.forename: S
authors.initials: S
authors.lastname: Maragkoudakis
authors.affiliation: Department of Cardiology, Heraklion University Hospital, Heraklion, Greece.
authors.forename: H
authors.initials: H
authors.lastname: Nakou
authors.affiliation: Department of Cardiology, Heraklion University Hospital, Heraklion, Greece.
authors.forename: K
authors.initials: K
authors.lastname: Roufas
authors.affiliation: Department of Cardiology, Heraklion University Hospital, Heraklion, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Patrianakos
authors.affiliation: Division of Biostatistics, School of Medicine, University of Crete, Heraklion, Greece.
authors.forename: G
authors.initials: G
authors.lastname: Chlouverakis
authors.affiliation: Department of Clinical Chemistry-Biochemistry, Heraklion University Hospital, Heraklion, Greece.
authors.forename: N
authors.initials: N
authors.lastname: Malliaraki
authors.affiliation: Department of Cardiology, Heraklion University Hospital, Heraklion, Greece.
authors.forename: P E
authors.initials: PE
authors.lastname: Vardas
mesh_terms: D000368:Aged; D000419:Albuminuria / Q000097:blood / Q000175:diagnosis / Q000235:genetics* / Q000652:urine; D000074166:Circulating MicroRNA / Q000097:blood / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D005819:Genetic Markers; D006801:Humans; D006973:Hypertension / Q000097:blood / Q000175:diagnosis / Q000235:genetics* / Q000652:urine; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D016482:Urinalysis
chemical_list: D000074166:Circulating MicroRNA; D005819:Genetic Markers; C548234:MIRN133 microRNA, human; C550906:MIRN208 microRNA, human; D035683:MicroRNAs