title: Evaluation of Safety and Antioxidant Activity of Yellow Tea (Camellia sinensis) Extract for Application in Food.
abstract: The article presents an evaluation of the safety of yellow tea (Camellia sinensis) extract consumption and its antioxidant activity in an animal model. Wistar rats were exposed through diet to 2, 6, and 10 g yellow tea extract/kg feed for 90 days. No signs of toxicity and no differences in mean body weight gain in the treated and control rats were recorded throughout the experiment. No statistically significant differences in hematology findings and clinical chemistry parameters were observed between controls and treated groups. Microscopic examination of tissue sections revealed no pathology attributable to yellow tea extract intake. Lipid peroxidation level in the liver was slightly increased in medium-dose males and high-dose females and decreased in two female groups receiving 2 and 6 g/kg of the extract tested. Content of carbonyl groups in protein, as well as the basal level of DNA damage, was not changed. In a majority of rats, the activity of antioxidant enzymes was increased except superoxide dismutase in high-dose groups, glutathione peroxidase in high-dose females, glutathione reductase in low- and mid-dose groups, and glutathione S-transferase in mid-dose females and high-dose males. It could be concluded that rats tolerated well dietary treatment with yellow tea extract up to 0.8 g/kg b.w./day for 90 days. Results showed that yellow tea extract at the doses tested did not demonstrate adverse effects and improved the antioxidant status in the liver of rats.
authors.affiliation: 1 Department of Toxicology, Poznań University of Medical Sciences , Poznań, Poland .
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Kujawska
authors.affiliation: 1 Department of Toxicology, Poznań University of Medical Sciences , Poznań, Poland .
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Ewertowska
authors.affiliation: 2 Department of Pharmaceutical Biochemistry, Poznań University of Medical Sciences , Poznań, Poland .
authors.forename: Ewa
authors.initials: E
authors.lastname: Ignatowicz
authors.affiliation: 1 Department of Toxicology, Poznań University of Medical Sciences , Poznań, Poland .
authors.forename: Teresa
authors.initials: T
authors.lastname: Adamska
authors.affiliation: 2 Department of Pharmaceutical Biochemistry, Poznań University of Medical Sciences , Poznań, Poland .
authors.forename: Hanna
authors.initials: H
authors.lastname: Szaefer
authors.affiliation: 3 Department of Food Technology and Nutrition, Poznań University of Life Sciences , Poznań, Poland .
authors.forename: Anna
authors.initials: A
authors.lastname: Gramza-Michałowska
authors.affiliation: 3 Department of Food Technology and Nutrition, Poznań University of Life Sciences , Poznań, Poland .
authors.forename: Józef
authors.initials: J
authors.lastname: Korczak
authors.affiliation: 1 Department of Toxicology, Poznań University of Medical Sciences , Poznań, Poland .
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Jodynis-Liebert
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000009:adverse effects / Q000378:metabolism*; D028241:Camellia sinensis / Q000009:adverse effects / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D010936:Plant Extracts / Q000009:adverse effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005982:Glutathione Transferase
keywords: Camellia sinensis; antioxidant enzymes; clinical chemistry; hematology; lipid peroxidation