title: Schistosoma japonicum HSP60-derived peptide SJMHE1 suppresses delayed-type hypersensitivity in a murine model.
abstract: BACKGROUND
  Parasite-derived molecules with immunomodulatory properties, which have been optimised during host-parasite co-evolution, exhibit potential applications as novel immunotherapeutics. We have previously demonstrated that Schistosoma japonicum HSP60-derived peptide SJMHE1 induces CD4(+)CD25(+) regulatory T-cells (Tregs) and that adoptively transferred SJMHE1-induced CD4(+)CD25(+) Tregs inhibit delayed-type hypersensitivity (DTH) in mice. However, multiple concerns regarding this method render this treatment unsuitable. To gain further insights into the potential effects of SJMHE1, we used ovalbumin (OVA)-induced DTH and evaluated the effect of SJMHE1 on DTH mice.
  
  
  METHODS
  BALB/c mice were sensitised with OVA alone or combined with SJMHE1 and then challenged with OVA to induce DTH. We first analysed the potential effects of SJMHE1 by measuring DTH responses, T-cell responses, cytokine secretion, and Treg proportions. We then evaluated the expression levels of IL-10 and TGF-β1 in CD4(+)CD25(+) T-cells during DTH and Treg generation to identify the mechanism by which SJMHE1 suppresses DTH.
  
  
  RESULTS
  SJMHE1 modulated the effector response against OVA-induced DTH and stimulated the production of the anti-inflammatory cytokines IL-10 and TGF-β1 in immunised mice through a mechanism involving CD4(+)CD25(+) Tregs. SJMHE1-induced CD4(+)CD25(+) Tregs expressed high levels of CTLA-4, IL-10, and TGF-β1, which substantially contributed to the suppressive activity during DTH. The administration of SJMHE1 to DTH in mice led to the expansion of CD4(+)CD25(+) Tregs from CD4(+)CD25(-) T-cells in the periphery, which inhibited DTH responses.
  
  
  CONCLUSIONS
  Our study proves that the parasite-driven peptide suppresses DTH in mice, which may confer a new option for inflammation treatment.
authors.affiliation: Department of Central Laboratory, The Affiliated Hospital of Jiangsu University, Zhenjiang, 212001, China. wangxuefeng1023@126.com.|Department of Nuclear Medicine and Institute of Oncology, The Affiliated Hospital of Jiangsu University, Zhenjiang, 212001, China. wangxuefeng1023@126.com.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Nuclear Medicine, The Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu, 212002, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Clinical Laboratory, Huai'an Hospital Affiliated of Xuzhou Medical College, Huaian, Jiangsu, 223300, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Department of Nuclear Medicine, The Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu, 212002, China.
authors.forename: Hongchang
authors.initials: H
authors.lastname: Ni
authors.affiliation: Department of Central Laboratory, The Affiliated Hospital of Jiangsu University, Zhenjiang, 212001, China.|Department of Nuclear Medicine and Institute of Oncology, The Affiliated Hospital of Jiangsu University, Zhenjiang, 212001, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Shi
authors.affiliation: Department of Central Laboratory, The Affiliated Hospital of Jiangsu University, Zhenjiang, 212001, China.|Department of Nuclear Medicine and Institute of Oncology, The Affiliated Hospital of Jiangsu University, Zhenjiang, 212001, China.
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Central Laboratory, The Affiliated Hospital of Jiangsu University, Zhenjiang, 212001, China.|Department of Nuclear Medicine and Institute of Oncology, The Affiliated Hospital of Jiangsu University, Zhenjiang, 212001, China.
authors.forename: Yuepeng
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Central Laboratory, The Affiliated Hospital of Jiangsu University, Zhenjiang, 212001, China.|Department of Nuclear Medicine and Institute of Oncology, The Affiliated Hospital of Jiangsu University, Zhenjiang, 212001, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Su
authors.affiliation: Department of Central Laboratory, The Affiliated Hospital of Jiangsu University, Zhenjiang, 212001, China.|Department of Nuclear Medicine and Institute of Oncology, The Affiliated Hospital of Jiangsu University, Zhenjiang, 212001, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Mou
authors.affiliation: Department of Central Laboratory, The Affiliated Hospital of Jiangsu University, Zhenjiang, 212001, China.|Department of Nuclear Medicine and Institute of Oncology, The Affiliated Hospital of Jiangsu University, Zhenjiang, 212001, China.
authors.forename: Deyu
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Central Laboratory, The Affiliated Hospital of Jiangsu University, Zhenjiang, 212001, China.|Department of Nuclear Medicine and Institute of Oncology, The Affiliated Hospital of Jiangsu University, Zhenjiang, 212001, China.
authors.forename: Chaoming
authors.initials: C
authors.lastname: Mao
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D018834:Chaperonin 60 / Q000008:administration & dosage* / Q000235:genetics / Q000494:pharmacology; D016207:Cytokines / Q000032:analysis; D004195:Disease Models, Animal; D015801:Helminth Proteins / Q000008:administration & dosage* / Q000235:genetics / Q000494:pharmacology; D006968:Hypersensitivity, Delayed / Q000517:prevention & control*; D007155:Immunologic Factors / Q000008:administration & dosage* / Q000235:genetics / Q000494:pharmacology; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D012549:Schistosoma japonicum / Q000235:genetics / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
chemical_list: D000485:Allergens; D018834:Chaperonin 60; D016207:Cytokines; D015801:Helminth Proteins; D007155:Immunologic Factors; D010047:Ovalbumin
keywords: Delayed-type hypersensitivity; SJMHE1; Schistosoma japonicum-derived peptide; Suppress