title: Immuno-enhancement of Taishan Pinus massoniana pollen polysaccharides on recombinant Bordetella avium ompA expressed in Pichia pastoris.
abstract: Bordetellosis, caused by Bordetella avium, continues to be an economic problem in the poultry industry of China. Vaccines with good protective ability are lacking. Thus, developing a novel vaccine against the B. avium infection is crucial. Here, we constructed a recombinant Pichia pastoris transformant capable of expressing the outer membrane protein A (ompA) of B. avium to prepare the recombinant ompA subunit vaccine and then evaluated its immune effects. To further investigate the immunomodulation effects of Taishan Pinus massoniana pollen polysaccharides (TPPPS) on this subunit vaccine, three concentrations (20, 40, and 60 mg/mL) of TPPPS were used as the adjuvants of the ompA subunit vaccine respectively. The conventional Freund's incomplete adjuvant served as the control of TPPPS. Chickens in different groups were separately vaccinated with these vaccines thrice. During the monitoring period, serum antibody titers, concentrations of serum IL-4, percentages of CD4(+) and CD8(+) T-lymphocytes in the peripheral blood, lymphocyte transformation rate, and protection rate were detected. Results showed that the pure ompA vaccine induced the production of anti-ompA antibody, the secretion of IL-4, the increase of CD4(+) T-lymphocytes counts and lymphocyte transformation rate in the peripheral blood. Moreover, the pure ompA vaccine provided a protection rate of 71.67% after the B. avium challenge. Notably, TPPPS adjuvant vaccines induced higher levels of immune responses than the pure ompA vaccine, and 60 mg/mL TPPPS adjuvant vaccine showed optimal immune effects and had a 91.67% protection rate. Our findings indicated that this recombinant B. avium ompA subunit vaccine combined with TPPPS had high immunostimulatory potential. Results provided a new perspective for B. avium subunit vaccine research.
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Taian, 271018, Shandong, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Taian, 271018, Shandong, China.
authors.forename: Cuilian
authors.initials: C
authors.lastname: Yu
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Taian, 271018, Shandong, China.
authors.forename: Chuanwen
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Taian, 271018, Shandong, China.
authors.forename: Mingxu
authors.initials: M
authors.lastname: Shao
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Taian, 271018, Shandong, China.
authors.forename: Zhengui
authors.initials: Z
authors.lastname: Yan
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Taian, 271018, Shandong, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Jiang
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Taian, 271018, Shandong, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Chi
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Taian, 271018, Shandong, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Taian, 271018, Shandong, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wei
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Taian, 271018, Shandong, China. Electronic address: zhurl@sdau.edu.cn.
authors.forename: Ruiliang
authors.initials: R
authors.lastname: Zhu
mesh_terms: D000276:Adjuvants, Immunologic / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D001885:Bordetella Infections / Q000517:prevention & control / Q000662:veterinary*; D042482:Bordetella avium / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002645:Chickens; D002681:China; D004337:Drug Carriers; D015847:Interleukin-4 / Q000097:blood; D010843:Pichia / Q000235:genetics; D028223:Pinus / Q000737:chemistry*; D011058:Pollen / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification / Q000378:metabolism*; D011201:Poultry Diseases / Q000517:prevention & control; D016896:Treatment Outcome; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D004337:Drug Carriers; D011134:Polysaccharides; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic; C078167:OMPA outer membrane proteins; D015847:Interleukin-4
keywords: Bordetella avium; OmpA; Pichia pastoris expression; Subunit vaccine; TPPPS