title: Comparison of 'HoBi'-like viral populations among persistent infected calves generated under experimental conditions and to inoculum virus.
abstract: Like other members from the Pestivirus genus, 'HoBi'-like pestiviruses cause economic losses for cattle producers due to both acute and persistent infections. The present study analyzed for the first time PI animals derived from a controlled infection with two different 'HoBi'-like strains where the animals were maintained under conditions where superinfection by other pestiviruses could be excluded. The sequence of the region coding for viral glycoproteins E1/E2 of variants within the swarms of viruses present in the PI calves and two viral inoculums used to generate them were compared. Differences in genetic composition of the viral swarms were observed suggesting that host factors can play a role in genetic variations among PIs. Moreover, PIs generated with the same inoculum showed amino acid substitutions in similar sites of the polyprotein, even in serum from PIs with different quasispecies composition, reinforcing that some specific sites in E2 are important for host adaptation.
authors.affiliation: Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil.
authors.forename: M N
authors.initials: MN
authors.lastname: Weber
authors.affiliation: Ruminant Diseases and Immunology Research Unit, Agricultural Research Service (ARS), National Animal Disease Center (NADC), United States Department of Agriculture (USDA), Ames, IA, United States.
authors.forename: F V
authors.initials: FV
authors.lastname: Bauermann
authors.affiliation: Ruminant Diseases and Immunology Research Unit, Agricultural Research Service (ARS), National Animal Disease Center (NADC), United States Department of Agriculture (USDA), Ames, IA, United States.
authors.forename: D O
authors.initials: DO
authors.lastname: Bayles
authors.affiliation: Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil.
authors.forename: C W
authors.initials: CW
authors.lastname: Canal
authors.affiliation: Ruminant Diseases and Immunology Research Unit, Agricultural Research Service (ARS), National Animal Disease Center (NADC), United States Department of Agriculture (USDA), Ames, IA, United States.
authors.forename: J D
authors.initials: JD
authors.lastname: Neill
authors.affiliation: Ruminant Diseases and Immunology Research Unit, Agricultural Research Service (ARS), National Animal Disease Center (NADC), United States Department of Agriculture (USDA), Ames, IA, United States. Electronic address: julia.ridpath@ars.usda.gov.
authors.forename: J F
authors.initials: JF
authors.lastname: Ridpath
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular; D004847:Epithelial Cells / Q000473:pathology / Q000821:virology; D015870:Gene Expression; D010576:Pestivirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D018182:Pestivirus Infections / Q000821:virology*; D010802:Phylogeny; D061251:Primary Cell Culture; D011994:Recombinant Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D014420:Turbinates / Q000473:pathology / Q000821:virology; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D011994:Recombinant Proteins; D014759:Viral Envelope Proteins
keywords: Persistent infection; Pestivirus; Quasispecies; Viral swarms; ‘HoBi’-like