title: Efficient production of polyhydroxyalkanoates (PHAs) from Pseudomonas mendocina PSU using a biodiesel liquid waste (BLW) as the sole carbon source.
abstract: Conditions for the optimal production of polyhydroxyalkanoate (PHA) by Pseudomonas mendocina PSU using a biodiesel liquid waste (BLW) were determined by response surface methodology. These were an initial carbon to nitrogen ratio (C/N) of 40 (mole/mole), an initial pH of 7.0, and a temperature of 35 °C. A biomass and PHA concentration of 3.65 g/L and about 2.6 g/L (77% DCW), respectively, were achieved in a growth associated process using 20 g/L glycerol in the BLW after 36 h of exponential growth. The PHA monomer compositions were 3HB (3-hydroxybutyrate), a short-chain-length-PHA, and the medium-chain-length-PHA e.g. 3-hydroxyoctanoate and 3-hydroxydecanoate. Both the phbC and phaC genes were characterized. The phbC enzyme had not been previously detected in a Pseudomonas mendocina species. A 2.15 g/L of an exopolysaccharide, alginate, was also produced with a similar composition to that of other Pseudomonas species.
authors.affiliation: a Faculty of Science, Department of Microbiology , Prince of Songkla University , Songkhla , Thailand.
authors.forename: Wankuson
authors.initials: W
authors.lastname: Chanasit
authors.affiliation: b Faculty of Pharmaceutical Sciences , Prince of Songkla University , Songkhla , Thailand.
authors.forename: Brian
authors.initials: B
authors.lastname: Hodgson
authors.affiliation: c Ecobiomaterial Research Laboratory, School of Biological Sciences , Universiti Sains Malaysia , Penang , Malaysia.
authors.forename: Kumar
authors.initials: K
authors.lastname: Sudesh
authors.affiliation: a Faculty of Science, Department of Microbiology , Prince of Songkla University , Songkhla , Thailand.
authors.forename: Kamontam
authors.initials: K
authors.lastname: Umsakul
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000096:biosynthesis; D000464:Alginates; D001673:Biodegradation, Environmental; D056804:Biofuels; D002210:Caprylates / Q000378:metabolism; D002244:Carbon / Q000378:metabolism*; D003652:Decanoic Acids / Q000378:metabolism; D005163:Factor Analysis, Statistical; D015870:Gene Expression; D005798:Genes, Bacterial; D020723:Glucuronic Acid / Q000096:biosynthesis; D005990:Glycerol / Q000378:metabolism; D006603:Hexuronic Acids; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D009584:Nitrogen / Q000378:metabolism; D010802:Phylogeny; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*; D044203:Pseudomonas mendocina / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D013696:Temperature
chemical_list: D000464:Alginates; D056804:Biofuels; D002210:Caprylates; D003652:Decanoic Acids; D006603:Hexuronic Acids; D007220:Industrial Waste; D054813:Polyhydroxyalkanoates; D002244:Carbon; D020723:Glucuronic Acid; C101940:3-hydroxyoctanoic acid; C017552:myrmicacin; D009584:Nitrogen; D005990:Glycerol; D020155:3-Hydroxybutyric Acid
keywords: Pseudomonas mendocina; biodiesel liquid waste (BLW); glycerol and Alginate; polyhydroxyalkanoate (PHA)