title: Isolation and Complete Genome Sequence of Algibacter alginolytica sp. nov., a Novel Seaweed-Degrading Bacteroidetes Bacterium with Diverse Putative Polysaccharide Utilization Loci.
abstract: The members of the phylum Bacteroidetes are recognized as some of the most important specialists for the degradation of polysaccharides. However, in contrast to research on Bacteroidetes in the human gut, research on polysaccharide degradation by marine Bacteroidetes is still rare. The genus Algibacter belongs to the Flavobacteriaceae family of the Bacteroidetes, and most species in this genus are isolated from or near the habitat of algae, indicating a preference for the complex polysaccharides of algae. In this work, a novel brown-seaweed-degrading strain designated HZ22 was isolated from the surface of a brown seaweed (Laminaria japonica). On the basis of its physiological, chemotaxonomic, and genotypic characteristics, it is proposed that strain HZ22 represents a novel species in the genus Algibacter with the proposed name Algibacter alginolytica sp. nov. The genome of strain HZ22, the type strain of this species, harbors 3,371 coding sequences (CDSs) and 255 carbohydrate-active enzymes (CAZymes), including 104 glycoside hydrolases (GHs) and 18 polysaccharide lyases (PLs); this appears to be the highest proportion of CAZymes (∼7.5%) among the reported strains in the class Flavobacteria Seventeen polysaccharide utilization loci (PUL) are predicted to be specific for marine polysaccharides, especially algal polysaccharides from red, green, and brown seaweeds. In particular, PUL N is predicted to be specific for alginate. Taking these findings together with the results of assays of crude alginate lyases, we prove that strain HZ22(T) can completely degrade alginate. This work reveals that strain HZ22(T) has good potential for the degradation of algal polysaccharides and that the structure and related mechanism of PUL in strain HZ22(T) are worth further research.
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, People's Republic of China.
authors.forename: Cong
authors.identifier: http://orcid.org/0000-0003-1587-0634
authors.initials: C
authors.lastname: Sun
authors.affiliation: Ocean College, Zhejiang University, Hangzhou, People's Republic of China.
authors.forename: Ge-Yi
authors.initials: GY
authors.lastname: Fu
authors.affiliation: Ocean College, Zhejiang University, Hangzhou, People's Republic of China.
authors.forename: Chong-Ya
authors.initials: CY
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.affiliation: Ocean College, Zhejiang University, Hangzhou, People's Republic of China.
authors.forename: Rui-Jun
authors.initials: RJ
authors.lastname: Wang
authors.affiliation: Ocean College, Zhejiang University, Hangzhou, People's Republic of China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Su
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, People's Republic of China.
authors.forename: Shuai-Bo
authors.initials: SB
authors.lastname: Han
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, People's Republic of China.
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Yu
authors.affiliation: Laboratory of Marine Ecosystem and Biogeochemistry, State Oceanic Administration, Hangzhou, People's Republic of China.|Second Institute of Oceanography, State Oceanic Administration, Hangzhou, People's Republic of China.
authors.forename: Hong
authors.identifier: http://orcid.org/0000-0002-3420-7777
authors.initials: H
authors.lastname: Cheng
authors.affiliation: School of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Linan, People's Republic of China.
authors.forename: Xin-Qi
authors.initials: XQ
authors.lastname: Zhang
authors.affiliation: Laboratory of Marine Ecosystem and Biogeochemistry, State Oceanic Administration, Hangzhou, People's Republic of China.|Second Institute of Oceanography, State Oceanic Administration, Hangzhou, People's Republic of China.
authors.forename: Ying-Yi
authors.initials: YY
authors.lastname: Huo
authors.affiliation: Laboratory of Marine Ecosystem and Biogeochemistry, State Oceanic Administration, Hangzhou, People's Republic of China wumin@zju.edu.cn xuxw@sio.org.cn.|Second Institute of Oceanography, State Oceanic Administration, Hangzhou, People's Republic of China.
authors.forename: Xue-Wei
authors.initials: XW
authors.lastname: Xu
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, People's Republic of China wumin@zju.edu.cn xuxw@sio.org.cn.|Ocean College, Zhejiang University, Hangzhou, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Wu
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D056426:Genetic Loci; D016680:Genome, Bacterial; D005838:Genotype; D007795:Laminaria / Q000378:metabolism* / Q000382:microbiology*; D053858:Metabolic Networks and Pathways / Q000235:genetics; D010802:Phylogeny; D011134:Polysaccharides / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D011134:Polysaccharides; D012336:RNA, Ribosomal, 16S