title: Rewiring Cas9 to Target New PAM Sequences.
abstract: Papers by Anders et al. (2016) and Hirano et al. (2016b), published in this issue of Molecular Cell, show that SpCas9 uses an induced fit mechanism to recognize altered protospacer adjacent motif (PAM) sequences.
authors.affiliation: Institute of Biotechnology, Vilnius University, Graiciuno 8, Vilnius LT-02241, Lithuania. Electronic address: siksnys@ibt.lt.
authors.forename: Virginijus
authors.initials: V
authors.lastname: Siksnys
authors.affiliation: Institute of Biotechnology, Vilnius University, Graiciuno 8, Vilnius LT-02241, Lithuania.
authors.forename: Giedrius
authors.initials: G
authors.lastname: Gasiunas
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D064113:CRISPR-Cas Systems; D021901:DNA, Intergenic / Q000235:genetics*; D004720:Endonucleases / Q000737:chemistry*; D000094704:RNA, Guide, CRISPR-Cas Systems
chemical_list: D001426:Bacterial Proteins; D021901:DNA, Intergenic; D004720:Endonucleases; D000094704:RNA, Guide, CRISPR-Cas Systems