title: Chemical composition and biological activities of Eruca vesicaria subsp. longirostris essential oils.
abstract: Context To date, there are no reports to validate the Tunisian traditional and folklore claims of Eruca vesicaria (L) Cav. subsp. longirostris (Brassicaceae) for the treatment of disease. Objective Investigation of the chemical composition antimicrobial and antioxidant activity of essential oils from Eruca longirostris leaves, stems, roots and fruits. Materials and methods The essential oils of E. longirostris from leaves, stems, roots and fruits were obtained after 4 h of hydrodistillation. Chemical compositions were determined using a combination of GC/FID and GC/MS. The in vitro antimicrobial activity of the volatile constituents of E. longirostris was performed in sterile 96-well microplates against three Gram-positive, four Gram-negative bacteria and one strain as yeast. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration values were reported. Furthermore, the antioxidant activity was evaluated by DPPH and ABTS assays. Results The main compound for fruits, stems and roots was the erucin (96.6%, 85.3% and 83.7%, respectively), while β-elemene (35.7%), hexahydrofarnesylacetone (23.9%), (E)-β-damascone (15.4%), erucin (10.6%) and α-longipinene (9.6%) constituted the major compounds in the essential oil of the leaves. The experimental results showed that in all tests, essential oil of fruits showed the better antioxidant activity than the others. On the other hand, the oils of stems, fruits and roots showed significant antimicrobial activity with MIC values ranging from 0.125 to 0.31 mg/mL against Candida species, Gram-positive and Gram-negative bacteria, mainly Salmonella enterica. Conclusions The present results indicate that essential oils of E. longirostris can be used as a source of erucin.
authors.affiliation: a Laboratoire Des Maladies Transmissibles Et Des Substances Biologiquement Actives, Faculté De Pharmacie , Monastir , Tunisia ;
authors.forename: Amel
authors.initials: A
authors.lastname: Omri Hichri
authors.affiliation: b Laboratoire De Bioressources: Biologie Integrative Et Valorisation (BIOLIVAL) , Institut Supérieur De Biotechnologie De Monastir, Université De Monastir , Monastir , Tunisia ;
authors.forename: Habib
authors.initials: H
authors.lastname: Mosbah
authors.affiliation: c Laboratoire De Biochimie, Mécanismes Moléculaires Et Maladie, Faculté De Médecine De Monastir , Université De Monastir , Monastir , Tunisia ;
authors.forename: Kaouther
authors.initials: K
authors.lastname: Majouli
authors.affiliation: a Laboratoire Des Maladies Transmissibles Et Des Substances Biologiquement Actives, Faculté De Pharmacie , Monastir , Tunisia ;
authors.forename: Malek
authors.initials: M
authors.lastname: Besbes Hlila
authors.affiliation: d Laboratoire De Chimie Hétérocyclique, Produits Naturels Et Réactivité, Equipe: Chimie Médicinale Et Produits Naturels, Faculté Des Sciences De Monastir , Université De Monastir , Monastir , Tunisia ;
authors.forename: Hichem
authors.initials: H
authors.lastname: Ben Jannet
authors.affiliation: e Dipartimento Di Farmacia , via Bonanno , Pisa , Italy.
authors.forename: Guido
authors.initials: G
authors.lastname: Flamini
authors.affiliation: a Laboratoire Des Maladies Transmissibles Et Des Substances Biologiquement Actives, Faculté De Pharmacie , Monastir , Tunisia ;
authors.forename: Mahjoub
authors.initials: M
authors.lastname: Aouni
authors.affiliation: b Laboratoire De Bioressources: Biologie Integrative Et Valorisation (BIOLIVAL) , Institut Supérieur De Biotechnologie De Monastir, Université De Monastir , Monastir , Tunisia ;
authors.forename: Boulbaba
authors.initials: B
authors.lastname: Selmi
mesh_terms: D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D019607:Brassicaceae / Q000737:chemistry; D002176:Candida albicans / Q000187:drug effects / Q000254:growth & development; D005410:Flame Ionization; D005638:Fruit; D008401:Gas Chromatography-Mass Spectrometry; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000302:isolation & purification / Q000494:pharmacology*; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D018515:Plant Leaves; D010938:Plant Oils / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots; D018547:Plant Stems; D010946:Plants, Medicinal; D013440:Sulfides / Q000302:isolation & purification / Q000494:pharmacology; D013451:Sulfonic Acids / Q000737:chemistry; D013861:Thiocyanates / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D009822:Oils, Volatile; D010851:Picrates; D010938:Plant Oils; D013440:Sulfides; D013451:Sulfonic Acids; D013861:Thiocyanates; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C073539:erucin; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Antimicrobial activity; Brassicaceae; antioxidant activity; chemical compounds identification