title: Chemical tags for site-specific fluorescent labeling of biomolecules.
abstract: This review focuses on the various approaches to covalently attach a chromophore to a biomolecule of interest in site-specific manner. Novel methods like inverse electron-demand Diels-Alder reaction, Pictet-Spengler ligation and enzyme tags like SNAP and Halo-tags are critically discussed and compared to established techniques like copper-free click reaction and native chemical ligation. Selected examples in which the tags have been exploited for in vitro or in vivo imaging are reviewed and evaluated.
authors.affiliation: Max Planck Institute for Polymer Research, Ackermannweg 10, 55128, Mainz, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Freidel
authors.affiliation: Max Planck Institute for Polymer Research, Ackermannweg 10, 55128, Mainz, Germany.
authors.forename: Stefka
authors.initials: S
authors.lastname: Kaloyanova
authors.affiliation: Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich Schiller University Jena, Jena Center of Soft Matter, Lessingstr. 8, 07743, Jena, Germany. kalina.peneva@uni-jena.de.
authors.forename: Kalina
authors.initials: K
authors.lastname: Peneva
mesh_terms: D057930:Click Chemistry / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D061848:Optical Imaging / Q000379:methods; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes
keywords: Bioorthogonal reactions; Chemical tags; Fluorescent dyes; Fluorescent labeling; Site-specific labeling