title: Roles of the Laodelphax striatellus Down syndrome cell adhesion molecule in Rice stripe virus infection of its insect vector.
abstract: The arthropod Down syndrome cell adhesion molecule (Dscam) mediates pathogen-specific recognition via an extensive protein isoform repertoire produced by alternative splicing. To date, most studies have focused on the subsequent pathogen-specific immune response, and few have investigated the entry into cells of viruses or endosymbionts. In the present study, we cloned and characterized the cDNA of Laodelphax striatellus Dscam (LsDscam) and investigated the function of LsDscam in rice stripe virus (RSV) infection and the influence on the endosymbiont Wolbachia. LsDscam displayed a typical Dscam domain architecture, including 10 immunoglobulin (Ig) domains, six fibronectin type III domains, one transmembrane domain and a cytoplasmic tail. Alternative splicing occurred at the N-termini of the Ig2 and Ig3 domains, the complete Ig7 domain, the transmembrane domain and the C-terminus, comprising 10, 51, 35, two and two variable exons, respectively. Potentially LsDscam could encode at least 71 400 unique isoforms and 17 850 types of extracellular regions. LsDscam was expressed in various L. striatellus tissues. Knockdown of LsDscam mRNA via RNA interference decreased the titres of both RSV and Wolbachia, but did not change the numbers of the extracellular symbiotic bacterium Acinetobacter rhizosphaerae. Specific Dscam isoforms may play roles in enhancing the infection of vector-borne viruses or endosymbionts.
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.|National Plant Gene Research Center, Beijing, China.|University of the Chinese Academy of Sciences, Beijing, China.
authors.forename: F
authors.initials: F
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.|National Plant Gene Research Center, Beijing, China.|University of the Chinese Academy of Sciences, Beijing, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Li
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.|National Plant Gene Research Center, Beijing, China.
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.|National Plant Gene Research Center, Beijing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Huo
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.|National Plant Gene Research Center, Beijing, China.
authors.forename: H
authors.initials: H
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.|National Plant Gene Research Center, Beijing, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Song
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: F
authors.initials: F
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.|National Plant Gene Research Center, Beijing, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.|National Plant Gene Research Center, Beijing, China.
authors.forename: R
authors.initials: R
authors.lastname: Fang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D006430:Hemiptera / Q000235:genetics / Q000254:growth & development / Q000382:microbiology* / Q000502:physiology*; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007303:Insect Vectors / Q000235:genetics / Q000254:growth & development / Q000382:microbiology / Q000502:physiology; D019006:Neural Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D009758:Nymph / Q000235:genetics / Q000254:growth & development / Q000382:microbiology / Q000502:physiology; D012275:Oryza / Q000821:virology*; D010935:Plant Diseases / Q000821:virology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D013559:Symbiosis; D029364:Tenuivirus / Q000502:physiology*; D020577:Wolbachia / Q000502:physiology
chemical_list: D018076:DNA, Complementary; D019476:Insect Proteins; D019006:Neural Cell Adhesion Molecules; D012333:RNA, Messenger
keywords: Down syndrome cell adhesion molecule; Laodelphax striatellus; rice stripe virus infection