title: [Somatostatin receptor expression in the ileal mucosa of patients with inflammatory bowel disease].
abstract: OBJECTIVE
  to investigate the specific features of somatostatin receptor expression in the ileal mucosa of patients with inflammatory bowel disease (IBD).
  
  
  METHODS
  Ileal biopsy specimens from 42 patients aged 22 to 55 years, including 16 patients with Crohn's disease (CD), 14 with indeterminate colitis of IBD unclassified (IBDU), and 12 with the unchanged ileal mucosa (a comparison group). Immunohistochemical examination was performed using anti-somatostatin receptor type 2 (SSTR-2) antibodies (Epitomics, USA). SSTR2-positively stained cells were counted per 100 epitheliocytes.
  
  
  RESULTS
  There were three receptor expression types: predominantly membranous, cytoplasmic, and mixed (membranous-cytoplasmic). Calculation of cells with membranous expression indicated that the median was 1 in the patients with Crohn's disease (interquartile range (IQR) 0.5), 3 in those with IBDU (IQR 2.5), and 2 (IQR 2) in the comparison group. These indicators were 1 (IQR 2), 3 (IQR 2), and 2 (IQR 1) for cytoplasmic expression and 5 (IQR 4), 2 (IQR 3), and 3 (IQR 2) for membranous-cytoplasmic one.
  
  
  CONCLUSIONS
  The number of SSTR-2-expressing cells was significantly smaller in the patients with Crohn's disease than that in those with the unchanged mucosa and in those with indeterminate (unclassified) colitis. There were three SSTR2 staining types: predominantly membranous, cytoplasmic, and mixed (membranous-cytoplasmic). The cells with membranous staining appeared to be neuroendocrine, those with cytoplasmic expression were intraepithelial T lymphocytes, and those with membranous-cytoplasmic expression were both neuroendocrine and epithelial.
authors.affiliation: Academician A.I. Strukov Department of Pathological Anatomy, I.M. Sechenov First Moscow State Medical University, Moscow, Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Tertychnyi
authors.affiliation: Department of Intermediate-Level Therapy, Medical Faculty, Ingush State University, Nazran, Russia.
authors.forename: Kh M
authors.initials: KM
authors.lastname: Akhrieva
authors.affiliation: Department of Pathological Anatomy, A.I. Evdokimov Moscow State University of Medicine and Dentistry, Ministry of Health of Russia, Moscow, Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Zayratyants
authors.affiliation: Academician A.I. Strukov Department of Pathological Anatomy, I.M. Sechenov First Moscow State Medical University, Moscow, Russia.
authors.forename: L S
authors.initials: LS
authors.lastname: Selivanova
authors.affiliation: Academician A.I. Strukov Department of Pathological Anatomy, I.M. Sechenov First Moscow State Medical University, Moscow, Russia.
authors.forename: T M
authors.initials: TM
authors.lastname: Birg
mesh_terms: D000328:Adult; D003093:Colitis, Ulcerative / Q000235:genetics* / Q000473:pathology; D003424:Crohn Disease / Q000235:genetics* / Q000473:pathology; D005260:Female; D015870:Gene Expression; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000235:genetics* / Q000473:pathology; D007413:Intestinal Mucosa / Q000473:pathology; D008297:Male; D008875:Middle Aged; D017481:Receptors, Somatostatin / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D017481:Receptors, Somatostatin; C574734:SSTR2 protein, human