title: FAST Real Time PCR for control of intra-species recycling in aquaculture feed, focused to the most relevant fish species farmed in Europe.
abstract: Recent regulations in animal feed composition prohibit intra-species recycling, the recycling of one given animal species to the same species, in order to avoid potential safety risks to human and animal health. These regulations have generated the need of their control in aquaculture by effective and specific analytical techniques. To date, most studies of species identification and detection in feedstuffs are focused on land species, but few studies are focused on species composition in fish feed. The present work describes five methodologies based in Real Time PCR for detection of the most relevant fish species farmed in Europe: gilthead sea bream (Sparus aurata); sea bass (Dicentrarchus labrax); turbot (Scophthalmus maximus); rainbow trout (Onchorynchus mykiss); and salmon (Salmo salar), in order to guarantee the intra-species recycling regulation in aquaculture feedstuffs.
authors.affiliation: Specialized Molecular Biology Unit, ANFACO-CECOPESCA, Vigo 36310, Pontevedra, Spain. Electronic address: montse@anfaco.es.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Espiñeira
authors.affiliation: Specialized Molecular Biology Unit, ANFACO-CECOPESCA, Vigo 36310, Pontevedra, Spain.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Vieites
mesh_terms: D000821:Animal Feed / Q000032:analysis / Q000592:standards*; D000818:Animals; D001492:Bass; D005060:Europe; D005398:Fisheries; D005412:Flatfishes; D017686:Oncorhynchus mykiss; D060888:Real-Time Polymerase Chain Reaction; D059027:Recycling; D015203:Reproducibility of Results; D020317:Salmo salar; D021541:Sea Bream; D017422:Sequence Analysis, DNA
keywords: Aquaculture; Feed; Feedstuffs; Gilthead sea bream (Sparus aurata); Rainbow trout (Onchorynchus mykiss); Real Time PCR; Salmon (Salmo salar); Sea bass (Dicentrarchus labrax); Turbot (Scophthalmus maximus)