title: Beyond PAINs: Chemotype Sensitivity of Protein Methyltransferases in Screens.
abstract: Screening of the relatively new target class, the lysine and arginine methyltransferases (MTases), presents unique challenges in the identification and confirmation of active chemical matter. Examination of high throughput screening data generated using Scintillation Proximity Assay (SPA) format for a number of protein MTase targets reveals sensitivity to both the known pan assay interference compounds (PAINS) and also other scaffolds not currently precedented as assay interferers. We find that, in general, true actives show significant selectivity within the MTase family. With the exception of slight modifications of SAM-like compounds, scaffolds that are observed frequently in multiple MTase assays should be viewed with caution and should be carefully validated before following up.
authors.affiliation: Lilly Research Laboratories, Eli Lilly and Company , Lilly Corporate Center, Indianapolis, Indiana 46285, United States.
authors.forename: Cen
authors.initials: C
authors.lastname: Gao
authors.affiliation: Lilly Research Laboratories, Eli Lilly and Company , Lilly Corporate Center, Indianapolis, Indiana 46285, United States.
authors.forename: Brandon J
authors.initials: BJ
authors.lastname: Margolis
authors.affiliation: Lilly Research Laboratories, Eli Lilly and Company , Lilly Corporate Center, Indianapolis, Indiana 46285, United States.
authors.forename: John M
authors.initials: JM
authors.lastname: Strelow
authors.affiliation: Research and Development, Eli Lilly and Company Ltd. , Sunninghill Road, Windlesham, Surrey GU20 6PH, United Kingdom.
authors.forename: Lewis R
authors.initials: LR
authors.lastname: Vidler
authors.affiliation: Lilly Research Laboratories, Eli Lilly and Company , Lilly Corporate Center, Indianapolis, Indiana 46285, United States.
authors.forename: Mary M
authors.initials: MM
authors.lastname: Mader
keywords: HTS; Methyltransferase; PAINS; epigenetics; promiscuity