title: Therapeutic effects of three trichothecenes in the silkworm infection assay with Candida albicans.
abstract: The silkworm infection assay is a useful method for directly evaluating the in vivo therapeutic effects of drug candidates. In the present study, 3 known trichothecenes, trichodermin, epiisororidin E, and verrucarin A, were evaluated as antifungal agents in the silkworm-Candida albicans assay. Trichodermin and epiisororidin E yielded effective therapeutic effects, while verrucarin A exhibited no efficacy in this assay system. These results strongly suggest that trichodermin and epiisororidin E are the lead compounds for developing a new antifungal agent.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kitasato University.
authors.forename: Ryuji
authors.initials: R
authors.lastname: Uchida
authors.forename: Shingo
authors.initials: S
authors.lastname: Namiguchi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ishijima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tomoda
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology* / Q000633:toxicity; D012831:Bombyx / Q000187:drug effects* / Q000196:embryology / Q000382:microbiology; D016466:CHO Cells; D002176:Candida albicans / Q000187:drug effects* / Q000472:pathogenicity; D002177:Candidiasis / Q000188:drug therapy* / Q000382:microbiology; D002470:Cell Survival / Q000187:drug effects; D003412:Cricetulus; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D055808:Drug Discovery / Q000379:methods*; D006801:Humans; D007814:Larva / Q000187:drug effects / Q000382:microbiology; D014243:Trichodermin / Q000494:pharmacology; D014255:Trichothecenes / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D000935:Antifungal Agents; D014255:Trichothecenes; D014243:Trichodermin; C005348:muconomycin A