title: A three-dimensional cell culture system as an in vitro canine mammary carcinoma model for the expression of connective tissue modulators.
abstract: In this study, derived complex carcinoma (CC) and simple carcinoma (SC) cell lines were established and cultured under two-dimensional (2D) and three-dimensional (3D) conditions. The 3D was performed in six-well AlgiMatrix™ (LifeTechnologies®, Carlsbad, CA, USA) scaffolds, resulting in spheroids sized 50-125 µm for CC and 175-200 µm for SC. Cell viability was demonstrated up to 14 days for both models. Epidermal growth factor receptor (EGFR) was expressed in CC and SC in both systems. However, higher mRNA and protein levels were observed in SC 2D and 3D systems when compared with CC (P < 0.005). The connective tissue modulators, metalloproteinases-1, -2, -9 and -13 (MMPs), relaxin receptors 1 and 2 (RXR1 and RXR2) and E-cadherin (CDH1) were quantitated. All were upregulated similarly when canine mammary tumour (CMT)-derived cell lines were cultured under 3D AlgiMatrix, except CDH1 that was downregulated (P < 0.005). These results are promising towards the used of 3D system to increase a high throughput in vitro canine tumour model.
authors.affiliation: Veterinary Medicine School, Department of Support, Production and Animal Health, University of São Paulo State, Laboratory of Animal Virology and Cell Culture, Araçatuba, Brazil.|Veterinary Medicine School, Department of Clinical, Surgery and Animal Reproduction, University of São Paulo State, Veterinary Hospital Section, Araçatuba, Brazil.
authors.forename: T C
authors.initials: TC
authors.lastname: Cardoso
authors.affiliation: Veterinary Medicine School, Department of Clinical, Surgery and Animal Reproduction, University of São Paulo State, Veterinary Hospital Section, Araçatuba, Brazil.
authors.forename: S S
authors.initials: SS
authors.lastname: Sakamoto
authors.affiliation: Veterinary Medicine School, Department of Clinical, Surgery and Animal Reproduction, University of São Paulo State, Veterinary Hospital Section, Araçatuba, Brazil.
authors.forename: D
authors.initials: D
authors.lastname: Stockmann
authors.affiliation: Veterinary Medicine School, Department of Clinical, Surgery and Animal Reproduction, University of São Paulo State, Veterinary Hospital Section, Araçatuba, Brazil.
authors.forename: T F B
authors.initials: TFB
authors.lastname: Souza
authors.affiliation: Department of de Veterinary Medicine, Faculdade Zotecnia e Engenharia de Alimentos-USP, Pirassununga, SP, Brazil.
authors.forename: H L
authors.initials: HL
authors.lastname: Ferreira
authors.affiliation: Veterinary Medicine School, Department of Support, Production and Animal Health, University of São Paulo State, Laboratory of Animal Virology and Cell Culture, Araçatuba, Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Gameiro
authors.affiliation: Veterinary Medicine School, Department of Support, Production and Animal Health, University of São Paulo State, Laboratory of Animal Virology and Cell Culture, Araçatuba, Brazil.
authors.forename: F V
authors.initials: FV
authors.lastname: Vieira
authors.affiliation: Veterinary Medicine School, Department of Support, Production and Animal Health, University of São Paulo State, Laboratory of Animal Virology and Cell Culture, Araçatuba, Brazil.
authors.forename: M J Q
authors.initials: MJQ
authors.lastname: Louzada
authors.affiliation: Veterinary Medicine School, Department of Clinical, Surgery and Animal Reproduction, University of São Paulo State, Veterinary Hospital Section, Araçatuba, Brazil.
authors.forename: A L
authors.initials: AL
authors.lastname: Andrade
authors.affiliation: Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, RS, Brazil.
authors.forename: E F
authors.initials: EF
authors.lastname: Flores
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival; D003238:Connective Tissue / Q000378:metabolism*; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D066246:ErbB Receptors / Q000378:metabolism; D005260:Female; D015674:Mammary Neoplasms, Animal / Q000378:metabolism*; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D018000:Receptors, Peptide / Q000378:metabolism; D054457:Tissue Scaffolds
chemical_list: D015820:Cadherins; D043562:Receptors, G-Protein-Coupled; D018000:Receptors, Peptide; C033395:relaxin receptors; D066246:ErbB Receptors; D053509:Matrix Metalloproteinase 13; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D020781:Matrix Metalloproteinase 1
keywords: cancer markers; canine mammary tumours; cell phenotype; in vitro 3D culture