title: Label-free and selective sensing of uric acid with gold nanoclusters as optical probe.
abstract: Clinically, the amount of uric acid (UA) in biological fluids is closely related to some diseases such as hyperuricemia and gout, thus it is of great significance to sense UA in clinical samples. In this work, red gold nanoclusters (AuNCs) with relatively high fluorescence quantum yield and strong fluorescence emission were facilely available using bovine serum albumin (BSA) as template. The fluorescence of BSA-protected AuNCs can be sensitively quenched by H2O2, which is further capable of sensing UA through the specific catalytic oxidation with uricase, since it generates stoichiometric quantity of H2O2 by-product. The proposed assay allows for the selective detection of UA in the range of 10-800 μM with a detection limit of 6.6 μM, which is applicable to sense UA in clinical samples with satisfactory results, suggesting its great potential for diagnostic purposes.
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Chongqing Key Laboratory of Biomedical Analysis (Southwest University), Chongqing Science & Technology Commission, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chang
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
authors.forename: Wen Bi
authors.initials: WB
authors.lastname: Wu
authors.affiliation: College of Physical Science and Technology, Southwest University, Chongqing 400715, China.
authors.forename: Pu
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Chongqing Key Laboratory of Biomedical Analysis (Southwest University), Chongqing Science & Technology Commission, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Shao Qing
authors.initials: SQ
authors.lastname: Lie
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China. Electronic address: chengzhi@swu.edu.cn.
authors.forename: Cheng Zhi
authors.initials: CZ
authors.lastname: Huang
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D002417:Cattle; D005453:Fluorescence; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D014503:Urate Oxidase / Q000737:chemistry; D014527:Uric Acid / Q000097:blood*
chemical_list: D014527:Uric Acid; D012710:Serum Albumin, Bovine; D006046:Gold; D006861:Hydrogen Peroxide; D014503:Urate Oxidase
keywords: Clinical application; Gold nanoclusters; Urate oxidase; Uric acid; Visual analysis