title: Screening of dietary antioxidants against mitochondria-mediated oxidative stress by visualization of intracellular redox state.
abstract: Mitochondrial impairment and the resulting generation of reactive oxygen species (ROS) have been associated with aging and its related pathological conditions. Recently, dietary antioxidants have gained significant attention as potential preventive and therapeutic agents against ROS-generated aging and pathological conditions. We previously demonstrated that food-derived antioxidants prevented intracellular oxidative stress under proteasome inhibition conditions, which was attributed to mitochondrial dysfunction and ROS generation, followed by cell death. Here, we further screened dietary antioxidants for their activity as redox modulators by visualization of the redox state using Redoxfluor, a fluorescent protein redox probe. Direct alleviation of ROS by antioxidants, but not induction of antioxidative enzymes, prevented mitochondria-mediated intracellular oxidation. The effective antioxidants scavenged mitochondrial ROS and suppressed cell death. Our study indicates that redox visualization under mitochondria-mediated oxidative stress is useful for screening potential antioxidants to counteract mitochondrial dysfunction, which has been implicated in aging and the pathogenesis of aging-related diseases.
authors.affiliation: a Division of Applied Life Sciences , Graduate School of Agriculture, Kyoto University , Kyoto , Japan.
authors.forename: Sunita
authors.initials: S
authors.lastname: Maharjan
authors.affiliation: a Division of Applied Life Sciences , Graduate School of Agriculture, Kyoto University , Kyoto , Japan.|b Research Unit for Physiological Chemistry , The Center for the Promotion of Interdisciplinary Education and Research, Kyoto University , Kyoto , Japan.
authors.forename: Yasuyoshi
authors.initials: Y
authors.lastname: Sakai
authors.affiliation: b Research Unit for Physiological Chemistry , The Center for the Promotion of Interdisciplinary Education and Research, Kyoto University , Kyoto , Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Hoseki
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004032:Diet; D031541:Fluorescence Resonance Energy Transfer; D008928:Mitochondria / Q000187:drug effects*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*
chemical_list: D000975:Antioxidants
keywords: dietary antioxidant; mitochondria; reactive oxygen species; redox visualization; screening method