title: Sweet potato NAC transcription factor, IbNAC1, upregulates sporamin gene expression by binding the SWRE motif against mechanical wounding and herbivore attack.
abstract: Sporamin is a tuberous storage protein with trypsin inhibitory activity in sweet potato (Ipomoea batatas Lam.), which accounts for 85% of the soluble protein in tubers. It is constitutively expressed in tuberous roots but is expressed in leaves only after wounding. Thus far, its wound-inducible signal transduction mechanisms remain unclear. In the present work, a 53-bp DNA region, sporamin wound-response cis-element (SWRE), was identified in the sporamin promoter and was determined to be responsible for the wounding response. Using yeast one-hybrid screening, a NAC domain protein, IbNAC1, that specifically bound to the 5'-TACAATATC-3' sequence in SWRE was isolated from a cDNA library from wounded leaves. IbNAC1 was constitutively expressed in root tissues and was induced earlier than sporamin following the wounding of leaves. Transgenic sweet potato plants overexpressing IbNAC1 had greatly increased sporamin expression, increased trypsin inhibitory activity, and elevated resistance against Spodoptera litura. We further demonstrated that IbNAC1 has multiple biological functions in the jasmonic acid (JA) response, including the inhibition of root formation, accumulation of anthocyanin, regulation of aging processes, reduction of abiotic tolerance, and overproduction of reactive oxygen species (ROS). Thus, IbNAC1 is a core transcription factor that reprograms the transcriptional response to wounding via the JA-mediated pathway in sweet potato.
authors.affiliation: Institute of Plant Biology, National Taiwan University, Taipei, 106, Taiwan.
authors.forename: Shi-Peng
authors.initials: SP
authors.lastname: Chen
authors.affiliation: Institute of Plant Biology, National Taiwan University, Taipei, 106, Taiwan.
authors.forename: I Winnie
authors.initials: IW
authors.lastname: Lin
authors.affiliation: College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
authors.forename: Xuanyang
authors.initials: X
authors.lastname: Chen
authors.affiliation: Institute of Plant Biology, National Taiwan University, Taipei, 106, Taiwan.
authors.forename: Yin-Hao
authors.initials: YH
authors.lastname: Huang
authors.affiliation: Institute of Plant Biology, National Taiwan University, Taipei, 106, Taiwan.
authors.forename: Shiao-Chi
authors.initials: SC
authors.lastname: Chang
authors.affiliation: Institute of Plant Biology, National Taiwan University, Taipei, 106, Taiwan.
authors.forename: Hui-Shan
authors.initials: HS
authors.lastname: Lo
authors.affiliation: Institute of Plant Biology, National Taiwan University, Taipei, 106, Taiwan.
authors.forename: Hseuh-Han
authors.initials: HH
authors.lastname: Lu
authors.affiliation: Institute of Plant Biology, National Taiwan University, Taipei, 106, Taiwan.
authors.forename: Kai-Wun
authors.initials: KW
authors.lastname: Yeh
mesh_terms: D020816:Amino Acid Motifs; D003517:Cyclopentanes / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D060434:Herbivory; D027723:Ipomoea batatas / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D054883:Oxylipins / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000378:metabolism; D015398:Signal Transduction; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D020798:Two-Hybrid System Techniques; D015854:Up-Regulation
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; D014157:Transcription Factors; C067142:sporamin protein, Ipomoea batatas; C011006:jasmonic acid
keywords: NAC transcription factor; anti-herbivore; sporamin; sweet potato; trypsin inhibitory activity; wounding