title: Detection and Phylogenetic Analysis of Porcine Deltacoronavirus in Korean Swine Farms, 2015.
abstract: This study applied molecular-based method to investigate the presence of porcine deltacoronavirus (PDCoV) in 59 commercial pig farms in South Korea. The results of RT-PCR screening on a relatively large collection of faeces samples (n = 681) from January 2013 to March 2015 did not reveal the presence of PDCoV until the end of 2014. However, on March 2015, PDCoV-positive samples (SL2, SL5) were detected from SL swine farm in Gyeongbuk province. The phylogenetic trees based on the complete spike- and nucleocapsid protein-coding genes showed that SL2 and SL5 closely related to the US PDCoV strains rather than those in China. Thought Korean strains of PDCoV isolated in 2014 (KNU14.04) and in 2015 (SL2 and SL5) grouped within US PDCoV cluster, the reconstruction of ancestral amino acid changes suggested that they are different.
authors.affiliation: Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Korea.
authors.forename: J H
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Korea.
authors.forename: H C
authors.initials: HC
authors.lastname: Chung
authors.affiliation: Department of Veterinary Microbiology and Infectious Diseases, Faculty of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi, Vietnam.
authors.forename: V G
authors.initials: VG
authors.lastname: Nguyen
authors.affiliation: Research Unit, Green Cross Veterinary Products, Yongin, Korea.
authors.forename: H J
authors.initials: HJ
authors.lastname: Moon
authors.affiliation: Viral Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea.
authors.forename: H K
authors.initials: HK
authors.lastname: Kim
authors.affiliation: Forensic Medicine Division, Daegu Institute, National Forensic Service, Chilgok, Korea.
authors.forename: S J
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Korea.
authors.forename: C H
authors.initials: CH
authors.lastname: Lee
authors.affiliation: Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Korea.
authors.forename: G E
authors.initials: GE
authors.lastname: Lee
authors.affiliation: Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Korea.
authors.forename: B K
authors.initials: BK
authors.lastname: Park
mesh_terms: D000818:Animals; D003332:Coronaviridae / Q000235:genetics / Q000302:isolation & purification*; D003333:Coronaviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D003967:Diarrhea / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D005243:Feces / Q000821:virology; D005260:Female; D019590:Nucleocapsid Proteins / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D056910:Republic of Korea / Q000453:epidemiology; D017422:Sequence Analysis, DNA / Q000662:veterinary; D064370:Spike Glycoprotein, Coronavirus / Q000235:genetics; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000821:virology
chemical_list: D019590:Nucleocapsid Proteins; D064370:Spike Glycoprotein, Coronavirus
keywords: South Korea; porcine deltacoronavirus; swine