title: The testis and ovary transcriptomes of the rock bream (Oplegnathus fasciatus): A bony fish with a unique neo Y chromosome.
abstract: The rock bream (Oplegnathus fasciatus) is considerably one of the most economically important marine fish in East Asia and has a unique neo-Y chromosome system that is a good model to study the sex determination and differentiation in fish. In the present study, we used Illumina sequencing technology (HiSeq2000) to sequence, assemble and annotate the transcriptome of the testis and ovary tissues of rock bream. A total of 40,004,378 (NCBI SRA database SRX1406649) and 53,108,992 (NCBI SRA database SRX1406648) high quality reads were obtained from testis and ovary RNA sequencing, respectively, and 60,421 contigs (with average length of 1301 bp) were obtained after de novo assembling with Trinity software. Digital gene expression analysis reveals 14,036 contigs that show gender-enriched expressional profile with either testis-enriched (237 contigs) or ovary-enriched (581 contigs) with RPKM > 100. There are 237 male- and 582 female-abundant expressed genes that show sex dimorphic expression. We hope that the gonad transcriptome and those gender-enriched transcripts of rock bream can provide some insight into the understanding of genome-wide transcriptome profile of teleost gonad tissue and give useful information in fish gonad development.
authors.affiliation: Key Lab of Mariculture and Enhancement of Zhejiang Province, Marine Fishery Institute of Zhejiang Province, 316100 Zhoushan, China; Marine and Fishery Institute, Zhejiang Ocean University, 316100 Zhoushan, Zhejiang Province, China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Xu
authors.affiliation: Center of Excellence for the Oceans, National Taiwan Ocean University, 20224 Keelung, Taiwan.
authors.forename: Kang-Ning
authors.initials: KN
authors.lastname: Shen
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, 266071 Qingdao, China.
authors.forename: Zhaofei
authors.initials: Z
authors.lastname: Fan
authors.affiliation: Marine and Fishery Institute, Zhejiang Ocean University, 316100 Zhoushan, Zhejiang Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, 266071 Qingdao, China.
authors.forename: Feng
authors.initials: F
authors.lastname: You
authors.affiliation: Key Lab of Mariculture and Enhancement of Zhejiang Province, Marine Fishery Institute of Zhejiang Province, 316100 Zhoushan, China.
authors.forename: Bao
authors.initials: B
authors.lastname: Lou
authors.affiliation: Department of Bioscience Technology, Chung Yuan Christian University, 32023 Chung-Li, Taiwan.
authors.forename: Chung-Der
authors.initials: CD
authors.lastname: Hsiao
keywords: Gonad transcriptome; Ovary; Rock bream; Testis