title: Influence of enamel matrix derivative on healing of root surfaces after bonding treatment and intentional replantation of vertically fractured roots.
abstract: OBJECTIVE
  The objectives of this study were to histopathologically evaluate cementum regeneration on root surfaces when an enamel matrix derivative (EMD) was used to bond a vertically fractured root and to evaluate the effectiveness of EMD in inhibiting root resorption.
  
  
  METHODS
  A total of 40 roots from 24 maxillary premolars in beagles were used. The root was vertically fractured using a chisel and mallet. Super-Bond was then used to bond the fractured root. In the experimental group, the root surface was treated with ethylenediaminetetraacetic acid and an EMD. The control group received no treatment. The root was then replanted in its original location. Histopathological observation and measurement using image-analyzing software were carried out after 8 weeks.
  
  
  RESULTS
  In the experimental group, shallow resorption cavities developed on the root planed surfaces with new acellular cementum appearing over them. In the control group, no new cementum was seen on the planed surfaces, and there was connective tissue joining the roots. In some of the samples, resorption and multinucleated giant cells were seen. The experimental group showed a significantly larger volume of cementum formation (P < 0.001), and the volume of root resorption was significantly smaller (P = 0.004).
  
  
  CONCLUSIONS
  When bonding and replanting tooth roots after a vertical fracture, the application of EMD was effective in regenerating cementum on root surfaces from which periodontal ligament had been lost in the area around the fracture line and in reducing the incidence of root resorption.
authors.affiliation: Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan. sugaya@den.hokudai.ac.jp.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Sugaya
authors.affiliation: Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan.
authors.forename: Mahito
authors.initials: M
authors.lastname: Tomita
authors.affiliation: Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan.
authors.forename: Youji
authors.initials: Y
authors.lastname: Motoki
authors.affiliation: Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Miyaji
authors.affiliation: Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan.
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Kawamami
mesh_terms: D000818:Animals; D001896:Boron Compounds; D003746:Dental Enamel Proteins; D004285:Dogs; D008689:Methacrylates; D008768:Methylmethacrylates; D010513:Periodontal Ligament; D014090:Tooth Replantation; D014092:Tooth Root
chemical_list: D001896:Boron Compounds; D003746:Dental Enamel Proteins; D008689:Methacrylates; D008768:Methylmethacrylates; C049495:Super-bond
keywords: enamel matrix derivative; intentional replantation; new cementum; root resorption; vertical root fracture