title: Evaluation of the RapidHIT™ 200 and RapidHIT GlobalFiler(®) Express kit for fully automated STR genotyping.
abstract: The RapidHIT™ 200 Human Identification System and RapidHIT GlobalFiler(®) Express kit were evaluated and validated for use with single-source reference samples. It was of primary interest to evaluate the system for its efficacy as an expert system and to estimate a first pass success rate, as well as to identify the technical variables impacting that result. While results indicated that this instrument/kit combination can be used to accurately type single-source buccal samples, substantial variability in sensitivity and intra-color balance were observed, as were multiple artifacts, requiring extensive manual editing of the profiles. Artifacts included dye "blobs" and spectral overlap (pull-up) peaks that often originated from relatively low intensity allele peaks. Reduced intra-color balance, in combination with low sensitivity, occasionally resulted in instances of allelic dropout. Overall, 50% of the buccal samples analyzed in this study would have been successfully typed to give full GlobalFiler(®) profiles without the need for manual review and editing.
authors.affiliation: Jan Bashinski DNA Laboratory, Bureau of Forensic Services, California Department of Justice, Richmond, CA 94804, USA. Electronic address: mavis.datechong@doj.ca.gov.
authors.forename: Mavis
authors.initials: M
authors.lastname: Date-Chong
authors.affiliation: Jan Bashinski DNA Laboratory, Bureau of Forensic Services, California Department of Justice, Richmond, CA 94804, USA. Electronic address: bill.hudlow@doj.ca.gov.
authors.forename: William R
authors.initials: WR
authors.lastname: Hudlow
authors.affiliation: Jan Bashinski DNA Laboratory, Bureau of Forensic Services, California Department of Justice, Richmond, CA 94804, USA. Electronic address: martin.buoncristiani@doj.ca.gov.
authors.forename: Martin R
authors.initials: MR
authors.lastname: Buoncristiani
mesh_terms: D016477:Artifacts; D016172:DNA Fingerprinting / Q000295:instrumentation*; D019075:Electrophoresis, Capillary; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D018895:Microsatellite Repeats; D009061:Mouth Mucosa / Q000166:cytology; D015203:Reproducibility of Results
chemical_list: D005819:Genetic Markers
keywords: GlobalFiler(®) Express; Modified Rapid DNA analysis; Rapid DNA; RapidHIT™ 200