title: Development of buffalo (Bubalus bubalis) embryonic stem cell lines from somatic cell nuclear transferred blastocysts.
abstract: We developed buffalo embryonic stem cell lines from somatic cell nuclear transfer derived blastocysts, produced by hand-guided cloning technique. The inner cell mass of the blastocyst was cut mechanically using a Microblade and cultured onto feeder cells in buffalo embryonic stem (ES) cell culture medium at 38 °C in a 5% CO2 incubator. The stem cell colonies were characterized for alkaline phosphatase activity, karyotype, pluripotency and self-renewal markers like OCT4, NANOG, SOX2, c-Myc, FOXD3, SSEA-1, SSEA-4, TRA-1-60, TRA-1-81 and CD90. The cell lines also possessed the capability to differentiate across all the three germ layers under spontaneous differentiation conditions.
authors.affiliation: Embryo Biotechnology Lab, Animal Biotechnology Center, National Dairy Research Institute, Karnal, India , 132001.
authors.forename: Syed Mohmad
authors.initials: SM
authors.lastname: Shah
authors.affiliation: Embryo Biotechnology Lab, Animal Biotechnology Center, National Dairy Research Institute, Karnal, India , 132001.
authors.forename: Neha
authors.initials: N
authors.lastname: Saini
authors.affiliation: Embryo Biotechnology Lab, Animal Biotechnology Center, National Dairy Research Institute, Karnal, India , 132001.
authors.forename: Syma
authors.initials: S
authors.lastname: Ashraf
authors.affiliation: Embryo Biotechnology Lab, Animal Biotechnology Center, National Dairy Research Institute, Karnal, India , 132001.
authors.forename: Manoj K
authors.initials: MK
authors.lastname: Singh
authors.affiliation: Embryo Biotechnology Lab, Animal Biotechnology Center, National Dairy Research Institute, Karnal, India , 132001.
authors.forename: Radheysham
authors.initials: R
authors.lastname: Manik
authors.affiliation: Embryo Biotechnology Lab, Animal Biotechnology Center, National Dairy Research Institute, Karnal, India , 132001.
authors.forename: Suresh K
authors.initials: SK
authors.lastname: Singla
authors.affiliation: Embryo Biotechnology Lab, Animal Biotechnology Center, National Dairy Research Institute, Karnal, India , 132001.
authors.forename: Prabhat
authors.initials: P
authors.lastname: Palta
authors.affiliation: Embryo Biotechnology Lab, Animal Biotechnology Center, National Dairy Research Institute, Karnal, India , 132001.
authors.forename: Manmohan Singh
authors.initials: MS
authors.lastname: Chauhan
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism*; D002020:Buffaloes; D002454:Cell Differentiation; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans