title: Active site specificity profiling datasets of matrix metalloproteinases (MMPs) 1, 2, 3, 7, 8, 9, 12, 13 and 14.
abstract: The data described provide a comprehensive resource for the family-wide active site specificity portrayal of the human matrix metalloproteinase family. We used the high-throughput proteomic technique PICS (Proteomic Identification of protease Cleavage Sites) to comprehensively assay 9 different MMPs. We identified more than 4300 peptide cleavage sites, spanning both the prime and non-prime sides of the scissile peptide bond allowing detailed subsite cooperativity analysis. The proteomic cleavage data were expanded by kinetic analysis using a set of 6 quenched-fluorescent peptide substrates designed using these results. These datasets represent one of the largest specificity profiling efforts with subsequent structural follow up for any protease family and put the spotlight on the specificity similarities and differences of the MMP family. A detailed analysis of this data may be found in Eckhard et al. (2015) [1]. The raw mass spectrometry data and the corresponding metadata have been deposited in PRIDE/ProteomeXchange with the accession number PXD002265.
authors.affiliation: Centre for Blood Research, Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Eckhard
authors.affiliation: Centre for Blood Research, Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Pitter F
authors.initials: PF
authors.lastname: Huesgen
authors.affiliation: Centre for Blood Research, Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Oliver
authors.initials: O
authors.lastname: Schilling
authors.affiliation: Centre for Blood Research, Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Caroline L
authors.initials: CL
authors.lastname: Bellac
authors.affiliation: Centre for Blood Research, Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Georgina S
authors.initials: GS
authors.lastname: Butler
authors.affiliation: Centre for Blood Research, Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Jennifer H
authors.initials: JH
authors.lastname: Cox
authors.affiliation: Centre for Blood Research, Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Antoine
authors.initials: A
authors.lastname: Dufour
authors.affiliation: Centre for Blood Research, Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Verena
authors.initials: V
authors.lastname: Goebeler
authors.affiliation: Centre for Blood Research, Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Reinhild
authors.initials: R
authors.lastname: Kappelhoff
authors.affiliation: Centre for Blood Research, Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Auf dem Keller
authors.affiliation: Centre for Blood Research, Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Theo
authors.initials: T
authors.lastname: Klein
authors.affiliation: Centre for Blood Research, Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Philipp F
authors.initials: PF
authors.lastname: Lange
authors.affiliation: Centre for Blood Research, Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Giada
authors.initials: G
authors.lastname: Marino
authors.affiliation: Centre for Blood Research, Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Charlotte J
authors.initials: CJ
authors.lastname: Morrison
authors.affiliation: Centre for Blood Research, Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Anna
authors.initials: A
authors.lastname: Prudova
authors.affiliation: Centre for Blood Research, Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, BC, Canada.
authors.forename: David
authors.initials: D
authors.lastname: Rodriguez
authors.affiliation: Centre for Blood Research, Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Amanda E
authors.initials: AE
authors.lastname: Starr
authors.affiliation: Centre for Blood Research, Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Yili
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Centre for Blood Research, Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, BC, Canada; Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Overall
keywords: Cleavage sites; MMPs; Matrix metalloproteinases; PICS; Proteomics; Quenched fluorescence; Specificity profiling