title: Insulin-Like Growth Factor-I Increases Laminin, Integrin Subunits and Metalloprotease ADAM12 in Mouse Myoblasts.
abstract: The extracellular matrix (ECM) is considered a part of the myogenesis signaling mechanism. we hypothesized that insulin-like growth factor-I (IGF-I) modifies ECM during differentiation of mouse C2C12 cells. The myogenic effect of IGF-I (30 nmol/l) was manifested by increased myogenin and myosin heavy chain (MyHC) levels as well as fusion index (2.6 times over control) on the 3rd day of differentiation. IGF-I markedly augmented laminin, but not fibronectin. Cellular contents of integrin α3, α5 and β1 during 3-day differentiation increased in the presence of IGF-I. Treatment with IGF-I increased the expression of the long form of metalloprotease ADAM12 (100 kDa) in myocytes. In conclusion: i) IGF-I caused an increase of laminin, integrin α3 and β1 in C2C12 myogenic cells that can be secondary to stimulation of myogenesis; ii) IGF-I augmented integrin α5 and ADAM12 levels, suggesting a role of this growth factor in determination of the pool of reserve cells during myogenesis.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Grzelkowska-Kowalczyk
authors.forename: Kamil
authors.initials: K
authors.lastname: Grabiec
authors.forename: Justyna
authors.initials: J
authors.lastname: Tokarska
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Gajewska
authors.forename: Maciej
authors.initials: M
authors.lastname: Błaszczyk
authors.forename: Marta
authors.initials: M
authors.lastname: Milewska
mesh_terms: D051722:ADAM Proteins / Q000235:genetics / Q000378:metabolism*; D000072199:ADAM12 Protein; D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation / Q000502:physiology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D016023:Integrins / Q000235:genetics / Q000378:metabolism*; D007797:Laminin / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D024510:Muscle Development / Q000502:physiology; D032446:Myoblasts / Q000378:metabolism*; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism
chemical_list: D016023:Integrins; D007797:Laminin; D008565:Membrane Proteins; D021122:Protein Subunits; D007334:Insulin-Like Growth Factor I; D051722:ADAM Proteins; D000072199:ADAM12 Protein