title: Validation of a headspace trap gas chromatography and mass spectrometry method for the quantitative analysis of volatile compounds from degraded rapeseed oil.
abstract: Due to lipid oxidation, off-flavors, characterized by a fishy odor, are emitted during the heating of rapeseed oil in a fryer and affect the flavor of rapeseed oil even at low concentrations. Thus, there is a need for analytical methods to identify and quantify these products. To study the headspace composition of degraded rapeseed oil, and more specifically the compounds responsible for the fishy odor, a headspace trap gas chromatography with mass spectrometry method was developed and validated. Six volatile compounds formed during the degradation of rapeseed oil were quantified: 1-penten-3-one, (Z)-4-heptenal, hexanal, nonanal, (E,E)-heptadienal, and (E)-2-heptenal. Validation using accuracy profiles allowed us to determine the valid ranges of concentrations for each compound, with acceptance limits of 40% and tolerance limits of 80%. This method was then successfully applied to real samples of degraded oils.
authors.affiliation: Lesieur, R&D Center, Coudekerque-Branche, France.|Department of Analytical, Bioanalytical Sciences and Miniaturization (LSABM), Institute of Chemistry, Biology and Innovation (CBI) - ESPCI ParisTech, PSL* Research University, Paris, France.|AgroParisTech, UMR1145 GENIAL Analytical Chemistry Laboratory, Paris, France.
authors.forename: Lilia
authors.initials: L
authors.lastname: Sghaier
authors.affiliation: INRA, UMR1145 GENIAL Analytical Chemistry Laboratory, Paris, France.
authors.forename: Christophe B Y
authors.initials: CB
authors.lastname: Cordella
authors.affiliation: AgroParisTech, UMR1145 GENIAL Analytical Chemistry Laboratory, Paris, France.
authors.forename: Douglas N
authors.initials: DN
authors.lastname: Rutledge
authors.affiliation: Lesieur, R&D Center, Coudekerque-Branche, France.
authors.forename: Mickaël
authors.initials: M
authors.lastname: Watiez
authors.affiliation: Lesieur, R&D Center, Coudekerque-Branche, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Breton
authors.affiliation: Department of Analytical, Bioanalytical Sciences and Miniaturization (LSABM), Institute of Chemistry, Biology and Innovation (CBI) - ESPCI ParisTech, PSL* Research University, Paris, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Sassiat
authors.affiliation: Department of Analytical, Bioanalytical Sciences and Miniaturization (LSABM), Institute of Chemistry, Biology and Innovation (CBI) - ESPCI ParisTech, PSL* Research University, Paris, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Thiebaut
authors.affiliation: Department of Analytical, Bioanalytical Sciences and Miniaturization (LSABM), Institute of Chemistry, Biology and Innovation (CBI) - ESPCI ParisTech, PSL* Research University, Paris, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Vial
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry; D000074262:Rapeseed Oil / Q000737:chemistry*; D055549:Volatile Organic Compounds / Q000032:analysis*
chemical_list: D000074262:Rapeseed Oil; D055549:Volatile Organic Compounds
keywords: Accuracy profile; Gas chromatography; Headspace Trap / Rapeseed oil; Volatile compounds