title: Plant Tandem CCCH Zinc Finger Proteins Interact with ABA, Drought, and Stress Response Regulators in Processing-Bodies and Stress Granules.
abstract: Although multiple lines of evidence have indicated that Arabidopsis thaliana Tandem CCCH Zinc Finger proteins, AtTZF4, 5 and 6 are involved in ABA, GA and phytochrome mediated seed germination responses, the interacting proteins involved in these processes are unknown. Using yeast two-hybrid screens, we have identified 35 putative AtTZF5 interacting protein partners. Among them, Mediator of ABA-Regulated Dormancy 1 (MARD1) is highly expressed in seeds and involved in ABA signal transduction, while Responsive to Dehydration 21A (RD21A) is a well-documented stress responsive protein. Co-immunoprecipitation (Co-IP) and bimolecular fluorescence complementation (BiFC) assays were used to confirm that AtTZF5 can interact with MARD1 and RD21A in plant cells, and the interaction is mediated through TZF motif. In addition, AtTZF4 and 6 could also interact with MARD1 and RD21A in Y-2-H and BiFC assay, respectively. The protein-protein interactions apparently take place in processing bodies (PBs) and stress granules (SGs), because AtTZF5, MARD1 and RD21A could interact and co-localize with each other and they all can co-localize with the same PB and SG markers in plant cells.
authors.affiliation: Department of Horticulture and Crop Science, The Ohio State University, Columbus, Ohio, United States of America.
authors.forename: Srimathi
authors.initials: S
authors.lastname: Bogamuwa
authors.affiliation: Department of Horticulture and Crop Science, The Ohio State University, Columbus, Ohio, United States of America.|Department of Molecular Genetics, The Ohio State University, Columbus, Ohio, United States of America.|Center for Applied Plant Sciences, The Ohio State University, Columbus, Ohio, United States of America.|Ohio Agriculture Research and Development Center, The Ohio State University, The Ohio State University, Columbus, Ohio, United States of America.
authors.forename: Jyan-Chyun
authors.initials: JC
authors.lastname: Jang
mesh_terms: D000040:Abscisic Acid / Q000502:physiology*; D017360:Arabidopsis / Q000502:physiology* / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D057056:Cysteine Proteases / Q000378:metabolism*; D003681:Dehydration / Q000503:physiopathology*; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics; D047468:Immunoprecipitation; D008856:Microscopy, Fluorescence; D015388:Organelles / Q000502:physiology; D010446:Peptide Fragments / Q000378:metabolism; D025941:Protein Interaction Mapping; D011523:Protoplasts / Q000378:metabolism / Q000648:ultrastructure; D012323:RNA Processing, Post-Transcriptional; D018749:RNA, Plant / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013312:Stress, Physiological / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D016335:Zinc Fingers
chemical_list: D000040:Abscisic Acid; D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D057056:Cysteine Proteases; D010446:Peptide Fragments; D018749:RNA, Plant; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C491109:MARD1 protein, Arabidopsis; C586036:TZF4 protein, Arabidopsis; C586037:TZF5 protein, Arabidopsis; C549570:RD21 protein, Arabidopsis; C111376:At5g07500 protein, Arabidopsis