title: A preliminary study on epigenetic regulation of Acanthopanax senticosus in leukemia cell lines.
abstract: Conventional chemotherapy for leukemia inevitably causes systemic toxicity. Acanthopanax senticosus, a naturally occurring herb used in traditional Chinese medicine, has been found to be a multipotent bioflavonoid with great potential in the prevention and treatment of malignant diseases. However, the mechanism underlying the action of A. senticosus in epigenetic regulation is poorly understood. In the study described here, we focused on the efficacy of A. senticosus in inducing apoptosis of leukemia cells and a possible mechanism. By evaluating the inhibition ratio and morphologic changes, we found that A. senticosus can inhibit growth and induce apoptosis of human leukemia HL-60 and HL60/ADM cells in a dose- and time-dependent manner. Furthermore, A. senticosus induced Fas ligand (FasL) expression and blocked the cell cycle in S phase. In addition, A. senticosus exhibited a potential for inhibition of histone deacetylase (HADC), which contributes to histone acetylation. It possibly resulted in the promotion of the expression of FasL. It is suggested that A. senticosus could be recognized as a new HDAC inhibitor which was able to reactivate aberrantly silenced genes. We discuss the clinical aspects of using A. senticosus for treatment of leukemia.
authors.affiliation: Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Wang
authors.affiliation: Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China. Electronic address: zhh_lj@163.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhong
authors.affiliation: Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Fang-Yuan
authors.initials: FY
authors.lastname: Chen
authors.affiliation: Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Min-Yue
authors.initials: MY
authors.lastname: Zhang
authors.affiliation: Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Jia-Yi
authors.initials: JY
authors.lastname: Cai
authors.affiliation: Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Ji-Hua
authors.initials: JH
authors.lastname: Zhong
mesh_terms: D000107:Acetylation; D015415:Biomarkers; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D028001:Eleutherococcus / Q000737:chemistry*; D044127:Epigenesis, Genetic / Q000187:drug effects*; D053222:Fas Ligand Protein / Q000378:metabolism; D018922:HL-60 Cells; D006657:Histones / Q000378:metabolism; D006801:Humans; D007938:Leukemia / Q000235:genetics* / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D015415:Biomarkers; D053222:Fas Ligand Protein; D006657:Histones; D010936:Plant Extracts