title: Cell-mediated drug delivery by gingival interdental papilla mesenchymal stromal cells (GinPa-MSCs) loaded with paclitaxel.
abstract: Gingival tissue is composed of cell types that contribute to the body's defense against many agents in oral environment, wound healing and tissue regeneration. Thanks to their easy and scarcely invasive withdrawal procedure, interdental papilla provide a good source of mesenchymal stromal cells (GinPa-MSCs). We isolated GinPa-MSCs and verified their ability to uptake/release the anticancer agent Paclitaxel (PTX).
  
  
  
  In vitro expanded GinPa-MSCs were characterized for CD markers by FACS, tested for differentiation ability and analyzed by TEM. Their ability to uptake/release PTX was assessed according to a standardized procedure.
  
  
  
  The CD expression and chondro-adipo-osteo differentiation ability confirmed the mesenchymal feature of GinPa-MSCs. Surprisingly, 28% of GinPa-MSCs expressed CD14 marker and had an impressive pinocytotic activity. GinPa-MSCs were able to take up and release a sufficient amount of PTX to demonstrate effective in vitro activity against pancreatic carcinoma cells, suggesting that the drug was not inactivated.
  
  
  
  The procedure to obtain MSCs from interdental papilla is less invasive than that used for both bone marrow and adipose tissue, GinPa-MSCs are easy to expand and can be efficiently loaded with PTX. Taken together these qualities suggest that GinPa-MSCs may prove to be a good tool for cell-mediated drug delivery in cancer, particularly if related to stomatognathic system.
authors.affiliation: a Department of Biomedical, Surgical and Dental Sciences , University of Milan , Milan , Italy.|b I.R.C.C.S. Istituto Ortopedico Galeazzi , Milan , Italy.
authors.forename: Anna Teresa
authors.initials: AT
authors.lastname: Brini
authors.affiliation: a Department of Biomedical, Surgical and Dental Sciences , University of Milan , Milan , Italy.|d Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, Maxillofacial and Dental Unit, Department of Biomedical, Surgical and Dental Sciences , University of Milan , Milan , Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Coccè
authors.affiliation: a Department of Biomedical, Surgical and Dental Sciences , University of Milan , Milan , Italy.
authors.forename: Lorena M Josè
authors.initials: LM
authors.lastname: Ferreira
authors.affiliation: a Department of Biomedical, Surgical and Dental Sciences , University of Milan , Milan , Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Giannasi
authors.affiliation: c Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, Unit of Orthodontics and Paediatric Dentistry, School of Dentistry, Department of Biomedical, Surgical and Dental Sciences , University of Milan , Milan , Italy.
authors.forename: Gianguido
authors.initials: G
authors.lastname: Cossellu
authors.affiliation: d Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, Maxillofacial and Dental Unit, Department of Biomedical, Surgical and Dental Sciences , University of Milan , Milan , Italy.
authors.forename: Aldo Bruno
authors.initials: AB
authors.lastname: Giannì
authors.affiliation: e Department of Medical Sciences and Diagnostic Integrated , S. Martino Hospital, University of Genoa , Genoa Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Angiero
authors.affiliation: a Department of Biomedical, Surgical and Dental Sciences , University of Milan , Milan , Italy.
authors.forename: Arianna
authors.initials: A
authors.lastname: Bonomi
authors.affiliation: f Department of Veterinary Medicine , University of Perugia , Perugia , Italy.
authors.forename: Luisa
authors.initials: L
authors.lastname: Pascucci
authors.affiliation: g CNR - Institute of Cell Biology and Neurobiology , Rome , Italy.
authors.forename: Maria Laura
authors.initials: ML
authors.lastname: Falchetti
authors.affiliation: h Laboratory of Clinical Pathology and Neurogenetic Medicine , Fondazione IRCCS Neurological Institute Carlo Besta , Milan , Italy.
authors.forename: Emilio
authors.initials: E
authors.lastname: Ciusani
authors.affiliation: i Department of Medical Biotechnology and Translational Medicine , University of Milan , Milan , Italy.
authors.forename: Gianpietro
authors.initials: G
authors.lastname: Bondiolotti
authors.affiliation: a Department of Biomedical, Surgical and Dental Sciences , University of Milan , Milan , Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Sisto
authors.affiliation: j Cellular Neurobiology Laboratory, Department of Cerebrovascular Diseases , IRCCS Neurological Institute C. Besta , Milan , Italy.
authors.forename: Giulio
authors.initials: G
authors.lastname: Alessandri
authors.affiliation: a Department of Biomedical, Surgical and Dental Sciences , University of Milan , Milan , Italy.
authors.forename: Augusto
authors.initials: A
authors.lastname: Pessina
authors.affiliation: a Department of Biomedical, Surgical and Dental Sciences , University of Milan , Milan , Italy.|c Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, Unit of Orthodontics and Paediatric Dentistry, School of Dentistry, Department of Biomedical, Surgical and Dental Sciences , University of Milan , Milan , Italy.
authors.forename: Giampietro
authors.initials: G
authors.lastname: Farronato
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D017239:Paclitaxel / Q000008:administration & dosage*; D010190:Pancreatic Neoplasms / Q000188:drug therapy; D014945:Wound Healing / Q000187:drug effects
chemical_list: D017239:Paclitaxel
keywords: CFPAC-1; drug delivery; gingival mesenchymal stromal cells; paclitaxel