title: Screening of Toxic Chemicals in a Single Drop of Human Whole Blood Using Ordered Mesoporous Carbon as a Mass Spectrometry Probe.
abstract: Surface-enhanced laser desorption/ionization (SELDI) is a versatile and high-throughput mass spectrometry (MS) technique that uses a probe for extraction, enrichment, desorption, and ionization of target analytes. Here we report ordered mesoporous carbon as a new SELDI probe for rapid screening and identification of trace amount of toxic chemicals in a single drop of human whole blood without complicated sample preparation procedures. We demonstrate that ordered mesoporous carbon not only can selectively enrich a wide variety of low-mass toxic compounds from whole blood samples but also can be used as an excellent matrix to assist the laser desorption/ionization process of small molecules with low background noise, high repeatability, and good salt tolerance. High sensitivity (detection limits at ppt levels) and good reproducibility for typical toxic compounds were obtained. With CMK-8 as a SELDI probe, we successfully identified and screened six perfluorinated compounds in a single drop of whole blood collected from workers in a perfluorochemical plant. The method was also validated with complex samples such as human urine and environmental water samples. With distinct advantages such as simplicity, rapidness, minimal sample requirement, and high reliability, this method keeps great promise for various aspects of application.
authors.affiliation: State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences , Beijing 100085, China.|State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, China.
authors.forename: Xiu
authors.initials: X
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences , Beijing 100085, China.|Institute of Environment and Health, Jianghan University , Wuhan 430056, China.|College of Resources and Environment, University of Chinese Academy of Sciences , Beijing 100190, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences , Beijing 100085, China.
authors.forename: Jianjie
authors.initials: J
authors.lastname: Fu
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Nie
authors.affiliation: State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences , Beijing 100085, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences , Beijing 100085, China.|College of Resources and Environment, University of Chinese Academy of Sciences , Beijing 100190, China.
authors.forename: Guibin
authors.initials: G
authors.lastname: Jiang
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D005466:Fluorocarbons / Q000097:blood* / Q000652:urine; D064368:Healthy Volunteers; D057166:High-Throughput Screening Assays; D006801:Humans; D007834:Lasers; D013058:Mass Spectrometry / Q000379:methods*; D015335:Molecular Probes / Q000737:chemistry*; D010316:Particle Size; D016062:Porosity; D013499:Surface Properties; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D005466:Fluorocarbons; D015335:Molecular Probes; D014874:Water Pollutants, Chemical; D002244:Carbon