title: [Protective Effects of Total Saponins from Panax japonicus on H₂O₂Induced Injury via Mitochondia Pathway in SH-SY5Y Cells].
abstract: OBJECTIVE
  To investigate the protective mechanism of total saponins from Panax japonicus (SPJ) on H₂O₂ induced injury in SH-SY5Y cells.
  
  
  METHODS
  SH-SY5Y cells were divided into three groups: blank control group, model group (600 µmol/L H₂O₂) and drug treatment groups. Different concentrations of SPJ (0.1, 1, 5 and 20 µg/mL) were incubated with SH-SY5Y cells for 12 hours prior to exposing to 600 µmol/L H₂O₂ for another 12 h. Mitochondrial membrane potential (MMP) was detected by JC-1 method. Protein expressions of Sirt1 , PGC-1α, Foxo3a, LC3-II and Beclin1 were detected by Western blotting.
  
  
  RESULTS
  Compared to the H₂O₂ model group, SPJ pretreatment significantly increased MMP level and enhanced the protein expressions of Sirt1, PGC-1α, Foxo3a, LC3-II and Beclin1.
  
  
  CONCLUSIONS
  SPJ exerts protective effect on H₂O₂ induced SH-SY5Y cell injury through mitochondria pathway.
authors.forename: Li-li
authors.initials: LL
authors.lastname: Deng
authors.forename: Jing-zhi
authors.initials: JZ
authors.lastname: Wan
authors.forename: Ding
authors.initials: D
authors.lastname: Yuan
authors.forename: Chang-cheng
authors.initials: CC
authors.lastname: Zhang
authors.forename: Geng
authors.initials: G
authors.lastname: Li
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
mesh_terms: D002460:Cell Line; D006801:Humans; D006861:Hydrogen Peroxide / Q000009:adverse effects*; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D005894:Panax / Q000737:chemistry*; D012503:Saponins / Q000494:pharmacology*
chemical_list: D012503:Saponins; D006861:Hydrogen Peroxide