title: Nanofiber Expansion of Umbilical Cord Blood Hematopoietic Stem Cells.
abstract: BACKGROUND
  The aim of this study was the ex vivo expansion of Umbilical Cord Blood hematopoietic stem cells on biocompatible nanofiber scaffolds.
  
  
  METHODS
  CD133+ hematopoietic stem cells were separated from umbilical cord blood using MidiMacs (positive selection) system by means of monocolonal antibody CD133 (microbeads); subsequently, flowcytometry method was done to assess the purity of separated cells. Isolated cells were cultured on plate (2 Dimensional) and fibronectin conjugated polyethersulfon nanofiber scaffold, simultaneously (3 Dimensional). Colony assay test was performed to show colonization ability of expanded cells.
  
  
  RESULTS
  Cell count analysis revealed that expansion of hematopoietic stem cells in 2dimensional (2D) environment was greater than 3dimensional (3D) condition (p= 0.01). Assessment of stem cell- phenotype after expansions was performed by flowcytometric analysis which is showed that the maintenance of CD133 marker in expanded cells in 3 dimensional condition were higher than expanded cells in 2 dimensional condition (p=0.01). Moreover, colony assay test was performed before and after of expansion to show colonization ability of expanded cells both in 3D and 2D culture and results revealed more ability of 3D culture compared with 2D culture (p= 0.03).
  
  
  CONCLUSIONS
  The results of current study confirmed that umbilical cord blood CD133+ haematopoietic stem cells are able to expand on fibronectin conjugated polyethersulfon scaffold. These findings indicated that 3D is a proper and valuable cell culture system for hematopoietic stem cells expansion, compared to 2D in invitro situation.
authors.affiliation: Department of Hematology and Blood Banking, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran.
authors.forename: F
authors.initials: F
authors.lastname: Eskandari
authors.affiliation: Department of Hematology and Blood Banking, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Allahverdi
authors.affiliation: Hematology-Oncology and Stem cell Transplantation Research Center, Tehran university of Medical Science, Tehran, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Nasiri
authors.affiliation: Department of Medical Laboratory Sciences, Faculty of Allied Medicine, Qazvin University of Medical Science, Qazvin, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Azad
authors.affiliation: Department of Hematology and Blood Banking, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran.
authors.forename: N
authors.initials: N
authors.lastname: Kalantari
authors.affiliation: Department of Hematology and Blood Banking, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Soleimani
authors.affiliation: Hematology and Oncology Research Center, Shahid Sadoughi University Medical Sciences and Health Services, Yazd, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Zare-Zardini
keywords: Nanofiber scaffold; Polyethersulfon; Umbilical cord blood