title: Poly(l-Lactic Acid)/Gelatin Fibrous Scaffold Loaded with Simvastatin/Beta-Cyclodextrin-Modified Hydroxyapatite Inclusion Complex for Bone Tissue Regeneration.
abstract: Recently, the application of nanostructured materials in the field of tissue engineering has garnered attention to mediate treatment and regeneration of bone defects. In this study, poly(l-lactic acid) (PLLA)/gelatin (PG) fibrous scaffolds are fabricated and β-cyclodextrin (βCD) grafted nano-hydroxyapatite (HAp) is coated onto the fibrous scaffold surface via an interaction between βCD and adamantane. Simvastatin (SIM), which is known to promote osteoblast viability and differentiation, is loaded into the remaining βCD. The specimen morphologies are characterized by scanning electron microscopy. The release profile of SIM from the drug loaded scaffold is also evaluated. In vitro proliferation and osteogenic differentiation of human adipose derived stem cells on SIM/HAp coated PG composite scaffolds is characterized by alkaline phosphatase (ALP) activity, mineralization (Alizarin Red S staining), and real time Polymerase chain reaction (PCR). The scaffolds are then implanted into rabbit calvarial defects and analyzed by microcomputed tomography for bone formation after four and eight weeks. These results demonstrate that SIM loaded PLLA/gelatin/HAp-(βCD) scaffolds promote significantly higher ALP activity, mineralization, osteogenic gene expression, and bone regeneration than control scaffolds. This suggests the potential application of this material toward bone tissue engineering.
authors.affiliation: Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, 37235, USA.
authors.forename: Jung Bok
authors.initials: JB
authors.lastname: Lee
authors.affiliation: Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, 02447, Republic of Korea.
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Kim
authors.affiliation: Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, 37235, USA.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Balikov
authors.affiliation: Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, 02447, Republic of Korea.
authors.forename: Min Soo
authors.initials: MS
authors.lastname: Bae
authors.affiliation: Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, 02447, Republic of Korea.
authors.forename: Dong Nyoung
authors.initials: DN
authors.lastname: Heo
authors.affiliation: Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, 02447, Republic of Korea.
authors.forename: Donghyun
authors.initials: D
authors.lastname: Lee
authors.affiliation: Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, 02447, Republic of Korea.
authors.forename: Hyun Joon
authors.initials: HJ
authors.lastname: Rim
authors.affiliation: Department of Oral and Maxillofacial Surgery, Kyung Hee University Dental Hospital at Gang-dong, Seoul, 05278, Republic of Korea.
authors.forename: Deok-Won
authors.initials: DW
authors.lastname: Lee
authors.affiliation: Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, 37235, USA.
authors.forename: Hak-Joon
authors.initials: HJ
authors.lastname: Sung
authors.affiliation: Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, 02447, Republic of Korea.
authors.forename: Il Keun
authors.initials: IK
authors.lastname: Kwon
mesh_terms: D000818:Animals; D001846:Bone Development / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D017886:Durapatite / Q000008:administration & dosage / Q000737:chemistry; D005780:Gelatin / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D010012:Osteogenesis / Q000187:drug effects*; D011091:Polyesters / Q000008:administration & dosage / Q000737:chemistry*; D011817:Rabbits; D019821:Simvastatin / Q000008:administration & dosage / Q000737:chemistry; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry; D014945:Wound Healing / Q000187:drug effects; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D011091:Polyesters; D047392:beta-Cyclodextrins; C033616:poly(lactide); D005780:Gelatin; D017886:Durapatite; D019821:Simvastatin; C031215:betadex
keywords: adamantine; adipose derived stem cell; beta-cyclodextrin; bone tissue engineering; simvastatin