title: Production of a single cyclic type of fructooligosaccharide structure by inulin-degrading Paenibacillus sp. LX16 newly isolated from Jerusalem artichoke root.
abstract: A novel inulin-degrading bacterium was isolated from a soil sample collected on Jerusalem artichoke roots. It is a Gram-positive, aerobic, motile and central endospore-forming straight rod, and exhibits phenotypic properties being consistent with its classification in the genus Paenibacillus. The predominant cellular fatty acids were anteiso-C15:0, C16:0 and anteiso-C17:0. This strain represents a novel species of the genus Paenibacillus on the basis of phenotypic data together with phylogenetic analysis, and it is here designated as LX16 and deposited in China centre for type collection, China (= CCTCC 2015256). Strain LX16 could produce a cyclofructooligosaccharide fructanotransferase catalysing the formation of one type of fructooligosaccharide (FOS) from inulin. The FOS was identified as a cyclofructooligosaccharide with a degree of polymerization of 6. Such homology in inulin degradation products may be beneficial for the functional FOS production.
authors.affiliation: School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Yao
authors.affiliation: School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China.
authors.forename: Jiqiang
authors.initials: J
authors.lastname: Guo
authors.affiliation: School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China.
authors.forename: Wenzhu
authors.initials: W
authors.lastname: Tang
authors.affiliation: School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Sun
authors.affiliation: School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China.
authors.forename: Yingmin
authors.initials: Y
authors.lastname: Hou
authors.affiliation: School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China.
authors.forename: Xianzhen
authors.initials: X
authors.lastname: Li
mesh_terms: D000332:Aerobiosis; D001711:Biotransformation; D002681:China; D003600:Cytosol / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis; D006368:Helianthus / Q000382:microbiology; D007444:Inulin / Q000378:metabolism*; D008124:Locomotion; D009844:Oligosaccharides / Q000378:metabolism*; D056507:Paenibacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D005227:Fatty Acids; D009844:Oligosaccharides; C116580:fructooligosaccharide; D007444:Inulin