title: Whole genome sequence of the emerging oomycete pathogen Pythium insidiosum strain CDC-B5653 isolated from an infected human in the USA.
abstract: Pythium insidiosum ATCC 200269 strain CDC-B5653, an isolate from necrotizing lesions on the mouth and eye of a 2-year-old boy in Memphis, Tennessee, USA, was sequenced using a combination of Illumina MiSeq (300 bp paired-end, 14 millions reads) and PacBio (10  Kb fragment library, 356,001 reads). The sequencing data were assembled using SPAdes version 3.1.0, yielding a total genome size of 45.6 Mb contained in 8992 contigs, N50 of 13 Kb, 57% G + C content, and 17,867 putative protein-coding genes. This Whole Genome Shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession JRHR00000000.
authors.affiliation: Emerging Pathogens Institute, University of Florida, Gainesville, FL, United States; Department of Plant Pathology, University of Florida, Gainesville, FL, United States.
authors.forename: Marina S
authors.initials: MS
authors.lastname: Ascunce
authors.affiliation: Emerging Pathogens Institute, University of Florida, Gainesville, FL, United States.
authors.forename: Jose C
authors.initials: JC
authors.lastname: Huguet-Tapia
authors.affiliation: Department of Biology and Genetics Institute, University of Florida, Gainesville, FL, United States.
authors.forename: Edward L
authors.initials: EL
authors.lastname: Braun
authors.affiliation: Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, United States.
authors.forename: Almudena
authors.initials: A
authors.lastname: Ortiz-Urquiza
authors.affiliation: Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, United States.
authors.forename: Nemat O
authors.initials: NO
authors.lastname: Keyhani
authors.affiliation: Emerging Pathogens Institute, University of Florida, Gainesville, FL, United States; Department of Plant Pathology, University of Florida, Gainesville, FL, United States.
authors.forename: Erica M
authors.initials: EM
authors.lastname: Goss
keywords: Genome sequencing; Human emerging pathogen; Oomycete; Pythiosis; Pythium insidiosum