title: Proteomic analysis allows for identifying targets of Yinchenwuling Powder in hyperlipidemic rats.
abstract: BACKGROUND
  Yinchenwuling Powder (YCL) is a traditional Chinese medicine formula originated from Synopsis of Golden Chamber, which is effective in positively modulating lipid levels in clinics. In this study, we utilized proteomic technology to identify the therapeutic targets of YCL on hyperlipidemic rats.
  
  
  METHODS
  We established hyperlipidemic model rats and administrated them with different doses of YCL extracts (0.35g/ml, 0.75g/ml and 1.5g/ml). Serum lipid levels were quantified and proteomic analysis was performed on plasma samples at the end of the study. Total plasma proteins were separated by two-dimensional electrophoresis (2-DE), and protein spots with 1.5-fold difference were excised and then analyzed by MALDI-TOF MS. Proteomic results were verified by Western blotting.
  
  
  RESULTS
  The results showed that the serum levels of TC, TG, and LDL-C were significantly decreased, while the HDL-C levels were significantly increased in different doses of YCL treatment groups. After being analyzed by 2-DE and MALDI-TOF MS, 12 proteins were identified. Eight proteins (T-kininogen, C3, C4, C4BPA, Igλ-2 chain C, Mbl2, Hpx and FGL1) were up-regulated in hyperlipidemic model rats, while four proteins (ApoE, ALB, TTR and VDBP) were up-regulated in the control and the YCL-treated rats. Two plasma proteins, ApoE and FGL1, involved in lipid metabolism, were confirmed by western blotting, and the results were consistent with the data from the proteomics results.
  
  
  CONCLUSIONS
  In this experiment, we identified 12 differentially-expressed plasma proteins associated with therapeutic effects of YCL. The functions of those proteins are related with lipid metabolism, blood coagulation, anti-inflammation and substance transport. This study provided a clue for the mechanism that underlies the therapeutic effect of YCL on lipid metabolism.
authors.affiliation: Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China. Electronic address: liruomeng1983@aliyun.com.
authors.forename: Ruomeng
authors.initials: R
authors.lastname: Li
authors.affiliation: Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China. Electronic address: 121570535@qq.com.
authors.forename: Linlin
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China. Electronic address: 373840809@qq.com.
authors.forename: Ning
authors.initials: N
authors.lastname: Wu
authors.affiliation: Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China. Electronic address: 1194430092@qq.com.
authors.forename: Ruiyi
authors.initials: R
authors.lastname: Wang
authors.affiliation: Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China. Electronic address: 564796567@qq.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Cao
authors.affiliation: Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China. Electronic address: qiuxyxy@126.com.
authors.forename: Xinjian
authors.initials: X
authors.lastname: Qiu
authors.affiliation: Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China. Electronic address: wdsh666@126.com.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Wang
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D006949:Hyperlipidemias / Q000188:drug therapy*; D008055:Lipids / Q000097:blood; D008516:Medicine, Chinese Traditional; D040901:Proteomics; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D004365:Drugs, Chinese Herbal; D008055:Lipids; C000614375:yinchenwuling
keywords: Hyperlipidemia; Lipid metabilism; Proteomic; Therapeutic targets; Yinchenwuling Powder