title: Association of Catalase and Glutathione Peroxidase 1 Polymorphisms with Chronic Hepatitis C Outcome.
abstract: The hepatic damage caused by hepatitis C virus (HCV) infection is associated with the host immune response and viral regulatory factors. Catalase (CAT) and glutathione peroxidase 1 (GPX1) are antioxidant enzymes located in the peroxisomes and mitochondria, respectively, and are responsible for the control of intracellular hydrogen peroxide levels. Polymorphisms in CAT (C-262T) and GPX1 (Pro198Leu) are correlated with serum levels and enzyme activity. This study aimed to investigate the association of genetic polymorphisms of CAT C-262T (rs1001179) and GPX1 Pro198Leu (rs1050450) with different stages of liver fibrosis and development of hepatocellular carcinoma (HCC). This study included 445 patients with chronic hepatitis C, of whom 139 patients had mild fibrosis (F0-F1), 200 had moderate/severe fibrosis (F2-F4), and 106 had HCC. Genotyping of SNPs was performed by real-time PCR using TaqMan probes. The Pro/Pro genotype of GPX1 was significantly associated with fibrosis severity, HCC, Child Pugh score, and BCLC staging. Additionally, patients carrying both CT+TT genotypes in the CAT gene and the Pro/Pro genotype in the GPX1 gene had higher risk for developing moderate/severe fibrosis or HCC (p = 0.009, OR 2.40 and p = 0.002, OR 3.56, respectively). CAT and GPX1 polymorphisms may be implicated in the severity of liver fibrosis and HCC caused by HCV.
authors.affiliation: Instituto de Ciências Biológicas - ICB, Universidade de Pernambuco - UPE, Brazil.
authors.forename: Vanessa C S D
authors.initials: VC
authors.lastname: Sousa
authors.affiliation: Colegiado de Farmácia, Universidade Federal do Vale do São Francisco - UNIVASF, Brazil.|Rede Nordeste de Biotecnologia - RENORBIO, Brazil.
authors.forename: Rodrigo F
authors.initials: RF
authors.lastname: Carmo
authors.affiliation: Instituto do Fígado e Transplantes de Pernambuco - IFP, Brazil.|Centro de Pesquisas Aggeu Magalhães (FIOCRUZ), Brazil.
authors.forename: Luydson R S
authors.initials: LR
authors.lastname: Vasconcelos
authors.affiliation: Instituto do Fígado e Transplantes de Pernambuco - IFP, Brazil.|Faculdade de Ciências Médicas, UPE, Brazil.
authors.forename: Dayse C B L
authors.initials: DC
authors.lastname: Aroucha
authors.affiliation: Instituto do Fígado e Transplantes de Pernambuco - IFP, Brazil.|Faculdade de Ciências Médicas, UPE, Brazil.
authors.forename: Leila M M B
authors.initials: LM
authors.lastname: Pereira
authors.affiliation: Instituto de Ciências Biológicas - ICB, Universidade de Pernambuco - UPE, Brazil.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Moura
authors.affiliation: Instituto de Ciências Biológicas - ICB, Universidade de Pernambuco - UPE, Brazil.
authors.forename: Maria S M
authors.initials: MS
authors.lastname: Cavalcanti
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D006528:Carcinoma, Hepatocellular / Q000235:genetics; D002374:Catalase / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D005979:Glutathione Peroxidase / Q000235:genetics*; D019698:Hepatitis C, Chronic / Q000235:genetics*; D006801:Humans; D008103:Liver Cirrhosis / Q000235:genetics; D008113:Liver Neoplasms / Q000235:genetics; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D000094765:Glutathione Peroxidase GPX1
chemical_list: C577219:CAT protein, human; D002374:Catalase; D005979:Glutathione Peroxidase; D000094765:Glutathione Peroxidase GPX1; C000722067:GPX1 protein, human
keywords: CAT; GPX1; HCC; HCV; ROS