title: Effects of acute intra-abdominal hypertension on multiple intestinal barrier functions in rats.
abstract: Intra-abdominal hypertension (IAH) is a common and serious complication in critically ill patients for which there is no well-defined treatment strategy. Here, we explored the effect of IAH on multiple intestinal barriers and discussed whether the alteration in microflora provides clues to guide the rational therapeutic treatment of intestinal barriers during IAH. Using a rat model, we analysed the expression of tight junction proteins (TJs), mucins, chemotactic factors, and Toll-like receptor 4 (TLR4) by immunohistochemistry. We also analysed the microflora populations using 16S rRNA sequencing. We found that, in addition to enhanced permeability, acute IAH (20 mmHg for 90 min) resulted in significant disturbances to mucosal barriers. Dysbiosis of the intestinal microbiota was also induced, as represented by decreased Firmicutes (relative abundance), increased Proteobacteria and migration of Bacteroidetes from the colon to the jejunum. At the genus level, Lactobacillus species and Peptostreptococcaceae incertae sedis were decreased, whereas levels of lactococci remained unchanged. Our findings outline the characteristics of IAH-induced barrier changes, indicating that intestinal barriers might be treated to alleviate IAH, and the microflora may be an especially relevant target.
authors.affiliation: Department of Intensive Care Unit, Peking University Third Hospital, North Garden Road, No. 49, Haidian District, Beijing 100191, People's Republic of China.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Leng
authors.affiliation: Department of Intensive Care Unit, Peking University Third Hospital, North Garden Road, No. 49, Haidian District, Beijing 100191, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Yi
authors.affiliation: Department of Intensive Care Unit, Peking University Third Hospital, North Garden Road, No. 49, Haidian District, Beijing 100191, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Fan
authors.affiliation: Department of Intensive Care Unit, Peking University Third Hospital, North Garden Road, No. 49, Haidian District, Beijing 100191, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Department of Intensive Care Unit, Peking University Third Hospital, North Garden Road, No. 49, Haidian District, Beijing 100191, People's Republic of China.
authors.forename: Qinggang
authors.initials: Q
authors.lastname: Ge
authors.affiliation: Department of Intensive Care Unit, Peking University Third Hospital, North Garden Road, No. 49, Haidian District, Beijing 100191, People's Republic of China.
authors.forename: Gaiqi
authors.initials: G
authors.lastname: Yao
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development; D002630:Chemotactic Factors / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004195:Disease Models, Animal; D000069196:Gastrointestinal Microbiome / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology / Q000503:physiopathology*; D007422:Intestines / Q000382:microbiology / Q000503:physiopathology*; D059325:Intra-Abdominal Hypertension / Q000503:physiopathology*; D009077:Mucins / Q000378:metabolism; D010539:Permeability; D011157:Population Dynamics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017207:Rats, Sprague-Dawley; D017422:Sequence Analysis, DNA; D062725:Tight Junction Proteins / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000378:metabolism
chemical_list: D002630:Chemotactic Factors; D004269:DNA, Bacterial; D009077:Mucins; D012336:RNA, Ribosomal, 16S; D062725:Tight Junction Proteins; D051197:Toll-Like Receptor 4