title: CdSe/ZnS Quantum Dot-Labeled Lateral Flow Strips for Rapid and Quantitative Detection of Gastric Cancer Carbohydrate Antigen 72-4.
abstract: Carbohydrate antigen 72-4 (CA72-4) is an important biomarker associated closely with diagnosis and prognosis of early gastric cancer. How to realize quick, sensitive, specific, and quantitative detection of CA72-4 in clinical specimens has become a great requirement. Herein, we reported a CdSe/ZnS quantum dot-labeled lateral flow test strip combined with a charge-coupled device (CCD)-based reader was developed for rapid, sensitive, and quantitative detection of CA72-4. Two mouse monoclonal antibodies (mAbs) against CA72-4 were employed. One of them was coated as a test line, while another mAb was labeled with quantum dots and coated onto conjugate pad. The goat anti-mouse IgG was immobilized as a control line. After sample was added, a sandwich structure was formed with CA72-4 and these two mAbs. The fluorescent signal from quantum dots (QD)-labeled mAb in sandwich structure was related to the amount of detected CA72-4. A CCD-based reader was used to realize quantitative detection of CA72-4. Results showed that developed QD-labeled lateral flow strips to detect CA72-4 biomarker with the sensitivity of 2 IU/mL and 10 min detection time. One hundred sera samples from clinical patients with gastric cancer and healthy people were used to confirm specificity of this strip method; results showed that established strip method own 100 % reproducibility and 100 % specificity compared with Roche electrochemiluminescence assay results. In conclusion, CdSe/ZnS quantum dot-labeled lateral flow strips for detection of CA72-4 could realize rapid, sensitive, and specific detection of clinical samples and could own great potential in clinical translation in near future.
authors.affiliation: Out-Patient Department, Zhujiang Hospital, Southern Medical University, 253 Gongye Road, 510280, Guangzhou, Guangdong, People's Republic of China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Yan
authors.affiliation: Institute of Nano Biomedical and Engineering, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, 200240, Shanghai, People's Republic of China. wk_xa@163.com.|National Center for Translational Medicine, Collaborative Innovational Center for System Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, 200240, Shanghai, People's Republic of China. wk_xa@163.com.
authors.forename: Kan
authors.initials: K
authors.lastname: Wang
authors.affiliation: Out-Patient Department, Zhujiang Hospital, Southern Medical University, 253 Gongye Road, 510280, Guangzhou, Guangdong, People's Republic of China.
authors.forename: Wenting
authors.initials: W
authors.lastname: Lu
authors.affiliation: Institute of Nano Biomedical and Engineering, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, 200240, Shanghai, People's Republic of China.
authors.forename: Weijian
authors.initials: W
authors.lastname: Qin
authors.affiliation: Institute of Nano Biomedical and Engineering, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, 200240, Shanghai, People's Republic of China. dxcui@sjtu.edu.cn.|National Center for Translational Medicine, Collaborative Innovational Center for System Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, 200240, Shanghai, People's Republic of China. dxcui@sjtu.edu.cn.
authors.forename: Daxiang
authors.initials: D
authors.lastname: Cui
authors.affiliation: Out-Patient Department, Zhujiang Hospital, Southern Medical University, 253 Gongye Road, 510280, Guangzhou, Guangdong, People's Republic of China. hjh5258@foxmail.com.
authors.forename: Jinghua
authors.initials: J
authors.lastname: He
keywords: CA72-4; CdSe/ZnS quantum dots; Gastric cancer; Lateral flow strip