title: Aryl Diazonium Chemistry for the Surface Functionalization of Glassy Biosensors.
abstract: Nanostring resonator and fiber-optics-based biosensors are of interest as they offer high sensitivity, real-time measurements and the ability to integrate with electronics. However, these devices are somewhat impaired by issues related to surface modification. Both nanostring resonators and photonic sensors employ glassy materials, which are incompatible with electrochemistry. A surface chemistry approach providing strong and stable adhesion to glassy surfaces is thus required. In this work, a diazonium salt induced aryl film grafting process is employed to modify a novel SiCN glassy material. Sandwich rabbit IgG binding assays are performed on the diazonium treated SiCN surfaces. Fluorescently labelled anti-rabbit IgG and anti-rabbit IgG conjugated gold nanoparticles were used as markers to demonstrate the absorption of anti-rabbit IgG and therefore verify the successful grafting of the aryl film. The results of the experiments support the effectiveness of diazonium chemistry for the surface functionalization of SiCN surfaces. This method is applicable to other types of glassy materials and potentially can be expanded to various nanomechanical and optical biosensors.
authors.affiliation: Department of Electrical and Computer Engineering, University of Alberta, Edmonton, Alberta, AB T6G 2V4, Canada. wzheng2@ualberta.ca.
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Department of Electrical and Computer Engineering, University of Alberta, Edmonton, Alberta, AB T6G 2V4, Canada. Remko@ualberta.ca.
authors.forename: Remko
authors.initials: R
authors.lastname: van den Hurk
authors.affiliation: Department of Chemistry and National Institute for Nanotechnology, University of Alberta, Edmonton, Alberta, AB T6G 2G2, Canada. yong2@ualberta.ca.
authors.forename: Yong
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Chemistry and National Institute for Nanotechnology, University of Alberta, Edmonton, Alberta, AB T6G 2G2, Canada. rdu@ualberta.ca.
authors.forename: Rongbing
authors.initials: R
authors.lastname: Du
authors.affiliation: Department of Experimental Oncology, Cross Cancer Institute, University of Alberta, Edmonton, Alberta, AB T6G 1Z2, Canada. xuejun.sun@ualberta.ca.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Alberta, AB T6G 1H9, Canada. yiyu@ualberta.ca.
authors.forename: Yiyu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Chemistry and National Institute for Nanotechnology, University of Alberta, Edmonton, Alberta, AB T6G 2G2, Canada. mmcdermo@ualberta.ca.
authors.forename: Mark T
authors.initials: MT
authors.lastname: McDermott
authors.affiliation: Department of Electrical and Computer Engineering, University of Alberta, Edmonton, Alberta, AB T6G 2V4, Canada. sevoy@ualberta.ca.
authors.forename: Stephane
authors.initials: S
authors.lastname: Evoy
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D003979:Diazonium Compounds / Q000737:chemistry*; D005139:Eyeglasses; D006046:Gold; D053768:Metal Nanoparticles / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D011817:Rabbits; D013499:Surface Properties
chemical_list: D003979:Diazonium Compounds; D006046:Gold
keywords: biosensors; diazonium; glasses; nanostrings; optical fibers; surface linkers