title: Synthesis and biological evaluation of salicylic acid conjugated isoxazoline analogues on immune cell proliferation and angiogenesis.
abstract: Mitogenicity is the ability of the natural or synthetic compounds to induce cell division or proliferation. A series of salicylic acid derivatives containing isoxazoline moiety (8a-j) were synthesized and their immunopharmacological activities targeting lymphocyte proliferation and angiogenesis were evaluated. The compounds 8a-j mitogenicity were investigated on immunological cells that include human peripheral blood lymphocytes and murine splenocytes in-vitro. The results implicate that among the series of 8a-j, compound 8e showed a potent proliferative response on both human and murine lymphocytes. The proliferative index of the compound 8e was comparable to the reference mitogen Con A and mitogenecity is due to increased secretion IL-2. In -vivo CAM and rat corneal angiogenesis assays were performed to assess the compound's effect on endothelial cell migration and proliferation which inferred that 8e also induces the proliferation of endothelial cells. The study reports the synthetic immunostimulatory and pro-angiogenic activity of novel mitogen 8e which could be translated into new drug in future.
authors.affiliation: Department of Chemistry, Yuvaraja's College, University of Mysore, Mysore, 570005 Karnataka, India; Department of Chemistry, JSS College of Arts, Commerce & Science, Ooty Road, Mysore, 570025, India.
authors.forename: Naveen
authors.initials: N
authors.lastname: Puttaswamy
authors.affiliation: Molecular Biomedicine Laboratory, Postgraduate Department of Studies and Research in Biotechnology, Sahyadri Science College (Autonomous), Kuvempu University, Shimoga, 577203, Karnataka, India.
authors.forename: G S
authors.initials: GS
authors.lastname: Pavan Kumar
authors.affiliation: Department of Chemistry, Yuvaraja's College, University of Mysore, Mysore, 570005 Karnataka, India.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Al-Ghorbani
authors.affiliation: Molecular Biomedicine Laboratory, Postgraduate Department of Studies and Research in Biotechnology, Sahyadri Science College (Autonomous), Kuvempu University, Shimoga, 577203, Karnataka, India.
authors.forename: V
authors.initials: V
authors.lastname: Vigneshwaran
authors.affiliation: Molecular Biomedicine Laboratory, Postgraduate Department of Studies and Research in Biotechnology, Sahyadri Science College (Autonomous), Kuvempu University, Shimoga, 577203, Karnataka, India.
authors.forename: B T
authors.initials: BT
authors.lastname: Prabhakar
authors.affiliation: Department of Chemistry, Yuvaraja's College, University of Mysore, Mysore, 570005 Karnataka, India. Electronic address: shaukathara@yahoo.co.in.
authors.forename: Shaukath Ara
authors.initials: SA
authors.lastname: Khanum
mesh_terms: D000293:Adolescent; D000328:Adult; D043925:Angiogenesis Inducing Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects / Q000276:immunology; D057809:HEK293 Cells; D006801:Humans; D007155:Immunologic Factors / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D007555:Isoxazoles / Q000737:chemistry / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D008875:Middle Aged; D015394:Molecular Structure; D018919:Neovascularization, Physiologic / Q000187:drug effects* / Q000276:immunology; D051381:Rats; D020156:Salicylic Acid / Q000737:chemistry / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D013329:Structure-Activity Relationship; D055815:Young Adult
chemical_list: D043925:Angiogenesis Inducing Agents; D007155:Immunologic Factors; D007555:Isoxazoles; D020156:Salicylic Acid
keywords: IL-2; Isoxazaoline; Lymphocytes; Mitogenecity; Salicylic acid