title: Extracellular synthesis of silver and gold nanoparticles by Sporosarcina koreensis DC4 and their biological applications.
abstract: The present study highlights the microbial synthesis of silver and gold nanoparticles by Sporosarcina koreensis DC4 strain, in an efficient way. The synthesized nanoparticles were characterized by ultraviolet-visible spectrophotometry, which displayed maximum absorbance at 424nm and 531nm for silver and gold nanoparticles, respectively. The spherical shape of nanoparticles was characterized by field emission transmission electron microscopy. The energy dispersive X-ray spectroscopy and elemental mapping were displayed the purity and maximum elemental distribution of silver and gold elements in the respective nanoproducts. The X-ray diffraction spectroscopy results demonstrate the crystalline nature of synthesized nanoparticles. The particle size analysis demonstrate the nanoparticles distribution with respect to intensity, volume and number of nanoparticles. For biological applications, the silver nanoparticles have been explored in terms of MIC and MBC against pathogenic microorganisms such as Vibrio parahaemolyticus, Escherichia coli, Salmonella enterica, Bacillus anthracis, Bacillus cereus and Staphylococcus aureus. Moreover, the silver nanoparticles in combination with commercial antibiotics, such as vancomycin, rifampicin, oleandomycin, penicillin G, novobiocin, and lincomycin have been explored for the enhancement of antibacterial activity and the obtained results showed that 3μg concentration of silver nanoparticles sufficiently enhance the antimicrobial efficacy of commercial antibiotics against pathogenic microorganism. Furthermore, the silver nanoparticles potential has been reconnoitered for the biofilm inhibition by S. aureus, Pseudomonas aeruginosa and E. coli and the results revealed sufficient activity at 6μg concentration. In addition, gold nanoparticles have been applied for catalytic activity, for the reduction of 4-nitrophenol to 4-aminophenol using sodium borohydride and positive results were attained.
authors.affiliation: Department of Oriental Medicine Biotechnology and Ginseng Bank, College of Life Sciences, Kyung Hee University, Yongin 446-701, Republic of Korea.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Singh
authors.affiliation: Department of Oriental Medicine Biotechnology and Ginseng Bank, College of Life Sciences, Kyung Hee University, Yongin 446-701, Republic of Korea.
authors.forename: Hina
authors.initials: H
authors.lastname: Singh
authors.affiliation: Department of Oriental Medicine Biotechnology and Ginseng Bank, College of Life Sciences, Kyung Hee University, Yongin 446-701, Republic of Korea. Electronic address: yeonjukim@khu.ac.kr.
authors.forename: Yeon Ju
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Republic of Korea.
authors.forename: Ramya
authors.initials: R
authors.lastname: Mathiyalagan
authors.affiliation: Department of Oriental Medicine Biotechnology and Ginseng Bank, College of Life Sciences, Kyung Hee University, Yongin 446-701, Republic of Korea.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Oriental Medicine Biotechnology and Ginseng Bank, College of Life Sciences, Kyung Hee University, Yongin 446-701, Republic of Korea; Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Republic of Korea. Electronic address: dcyang@khu.ac.kr.
authors.forename: Deok Chun
authors.initials: DC
authors.lastname: Yang
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D006046:Gold / Q000737:chemistry* / Q000494:pharmacology; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests; D036103:Nanotechnology; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology; D058644:Sporosarcina / Q000145:classification / Q000235:genetics / Q000378:metabolism*
chemical_list: D000890:Anti-Infective Agents; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012834:Silver; D006046:Gold
keywords: Antimicrobial effect; Biofilm inhibition; Catalytic activity; Gold nanoparticles; Silver nanoparticles