title: Comparison of CBF1, CBF2, CBF3 and CBF4 expression in some grapevine cultivars and species under cold stress.
abstract: Grapevine, an important horticultural crop in the world, is moderately tolerant to cold conditions and is subjected to the cold injuries at different regions. So studies on different aspects of tolerance mechanism to unexpected cold of late spring as well as winter freezing seems necessary about this vine. For this reason, study on genes responsible for acquiring cold tolerance is very important. Transcription factors are among regulatory proteins that are responsible for cold acclimation. In this research work, expression levels of CBF1, CBF2, CBF3, and CBF4 transcription factors were studied on two cvs ofVitis vinifera("Khalili-Danedar" and "Shahroodi") as well as oneVitis ripariaat different times after treating at 4 °C. Results showed that twoviniferacultivars, "Khalili-Danedar" and "Shahroodi", had similar trend for each transcription factor. Gene expression increased at the beginning of cold stress and then decreased. Expression of these TF started some minutes (CBF1) after cold treatment and continued for several hours (CBF2), even till the tenth day (CBF4). All togetherV. ripariawhich is endemic to the cold regions behaved stronger and showed higher expression for all studied transcription factors. Among twoV. viniferacultivars, "Khalili-Danedar" showed significantly higher expression compared with "Shahroodi". The comparison of expression levels of these four transcription factors revealed that the least and the greatest expressions were recorded for CBF1 and CBF3 respectively, and two CBF2 and CBF4 had approximately the same expression levels.
authors.affiliation: University of Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Karimi
authors.affiliation: University of Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Ebadi
authors.affiliation: National Institue of Genetic Engineering and Biotechnology, Iran.
authors.forename: Seyed Amir
authors.initials: SA
authors.lastname: Mousavi
authors.affiliation: University of Tehran, Iran.
authors.forename: Seyed Alireza
authors.initials: SA
authors.lastname: Salami
authors.affiliation: Jahrom University, Iran.
authors.forename: Abdolkarim
authors.initials: A
authors.lastname: Zarei
keywords: CBF genes; Cold stress; Gene expression; Grape; Transcription factors