title: 1,4-Butanediol diglycidyl ether-cross-linked hyaluronan inhibits fibrosis in rat primary tenocytes by down-regulating autophagy modulation.
abstract: Epidural fibrosis, an inevitable part of the postoperative healing process, is one of the important causes of failed back surgery syndrome after spinal surgery. The aim of this study was to examine the inhibitory effect of a novel material 1,4-butanediol diglycidyl ether-cross-linked hyaluronan (cHA) on fibrosis in primary tenocytes. cHA inhibited migration, cell proliferation, and suppressed the expression of fibronectin, but not transforming growth factor-β, in primary tenocytes. cHA significantly increased matrix metalloproteinase-3 but decreased collagen-1 and microtubule-associated protein light chain 3-II expression in a dose-dependent manner compared with control groups. We therefore concluded that suppressing autophagy activity may be involved in the anti-fibrotic effect of cHA in primary tenocytes. Further, cHA may have the potential for preventing epidural fibrosis and subsequent failed back syndrome in patients with laminectomy in the future.
authors.affiliation: Department of Orthopedics, National Cheng Kung University Hospital, Tainan, Taiwan.
authors.forename: Dur-Zong
authors.initials: DZ
authors.lastname: Hsu
authors.affiliation: Department of Orthopedics, National Cheng Kung University Hospital, Tainan, Taiwan. jming@mail.ncku.edu.tw.|Department of Orthopedics, College of Medicine, National Cheng Kung University, 138 Sheng-Li Road, Tainan, 704, Taiwan. jming@mail.ncku.edu.tw.
authors.forename: I-Ming
authors.initials: IM
authors.lastname: Jou
mesh_terms: D000818:Animals; D001343:Autophagy / Q000187:drug effects*; D002072:Butylene Glycols / Q000737:chemistry* / Q000494:pharmacology; D002465:Cell Movement; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D005355:Fibrosis; D005786:Gene Expression Regulation / Q000187:drug effects; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000494:pharmacology; D008422:Materials Testing; D019278:Matrix Metalloproteinase 3 / Q000235:genetics / Q000378:metabolism; D051381:Rats; D000070916:Tenocytes / Q000187:drug effects*
chemical_list: D002072:Butylene Glycols; D024042:Collagen Type I; D006820:Hyaluronic Acid; C014376:1,4-bis(2,3-epoxypropoxy)butane; D019278:Matrix Metalloproteinase 3