title: Cartilage Oligomeric Matrix Protein Increases in Photodamaged Skin.
abstract: Cartilage oligomeric matrix protein (COMP) is a structural component of cartilage. Recent studies have described COMP as a pathogenic factor that promotes collagen deposition in fibrotic skin disorders such as scleroderma and keloid skin. Although collagen, a major dermis component, is thought to decrease in photoaged skin, recent reports have demonstrated the presence of tightly packed collagen fibrils with a structural resemblance to fibrosis in the papillary dermis of photoaged skin. Here we examined how photoaging damage relates to COMP expression and localization in photoaged skin. In situ hybridization revealed an increase in COMP-mRNA-positive cells with the progress of photoaging in preauricular skin (sun-exposed skin). The signal intensity of immunostaining for COMP increased with photoaging in not only the papillary dermis but also the reticular dermis affected by advancing solar elastosis. Immunoelectron microscopy detected the colocalization of COMP with both elastotic materials and collagen fibrils in photoaged skin. Ultraviolet light A irradiation of human dermal fibroblasts induced COMP expression at both the mRNA and protein levels. Ultraviolet light A-induced COMP expression was inhibited by an anti-transforming growth factor-β antibody or SB431542, an activin receptor-like kinase 5 inhibitor. These results suggest that the transforming growth factor-β-mediated upregulation of COMP expression may contribute to the modulation of dermal extracellular matrix in the photoaging process.
authors.affiliation: Biological Science Research, Kao Corporation, Odawara, Kanagawa, Japan; Department of Plastic Surgery, Tokai University School of Medicine, Isehara, Kanagawa, Japan. Electronic address: kobayashi.masaki2@kao.co.jp.
authors.forename: Masaki
authors.initials: M
authors.lastname: Kobayashi
authors.affiliation: Biological Science Research, Kao Corporation, Odawara, Kanagawa, Japan.
authors.forename: Keigo
authors.initials: K
authors.lastname: Kawabata
authors.affiliation: Biological Science Research, Kao Corporation, Odawara, Kanagawa, Japan.
authors.forename: Ayumi
authors.initials: A
authors.lastname: Kusaka-Kikushima
authors.affiliation: Health Beauty Products Research, Kao Corporation, Odawara, Kanagawa, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Sugiyama
authors.affiliation: Department of Dermatology, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
authors.forename: Tomotaka
authors.initials: T
authors.lastname: Mabuchi
authors.affiliation: Department of Cell Biology, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
authors.forename: Susumu
authors.initials: S
authors.lastname: Takekoshi
authors.affiliation: Department of Plastic Surgery, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
authors.forename: Muneo
authors.initials: M
authors.lastname: Miyasaka
authors.affiliation: Department of Dermatology, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Ozawa
authors.affiliation: Health Beauty Products Research, Kao Corporation, Odawara, Kanagawa, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Sakai
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000378:metabolism; D001707:Biopsy, Needle; D064236:Cartilage Oligomeric Matrix Protein / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007564:Japan; D008297:Male; D016253:Microscopy, Immunoelectron; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012016:Reference Values; D012720:Severity of Illness Index; D015595:Skin Aging / Q000473:pathology*; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D014466:Ultraviolet Rays / Q000009:adverse effects*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D064236:Cartilage Oligomeric Matrix Protein; D016212:Transforming Growth Factor beta