title: Distinct Contribution of Adult-Born Hippocampal Granule Cells to Context Encoding.
abstract: Adult-born granule cells (abGCs) have been implicated in cognition and mood; however, it remains unknown how these cells behave in vivo. Here, we have used two-photon calcium imaging to monitor the activity of young abGCs in awake behaving mice. We find that young adult-born neurons fire at a higher rate in vivo but paradoxically exhibit less spatial tuning than their mature counterparts. When presented with different contexts, mature granule cells underwent robust remapping of their spatial representations, and the few spatially tuned adult-born cells remapped to a similar degree. We next used optogenetic silencing to confirm the direct involvement of abGCs in context encoding and discrimination, consistent with their proposed role in pattern separation. These results provide the first in vivo characterization of abGCs and reveal their participation in the encoding of novel information.
authors.affiliation: Department of Neuroscience, Columbia University, New York, NY 10032, USA; Doctoral Program in Neurobiology and Behavior, Columbia University, New York, NY 10032, USA.
authors.forename: Nathan B
authors.initials: NB
authors.lastname: Danielson
authors.affiliation: Department of Neuroscience, Columbia University, New York, NY 10032, USA; Doctoral Program in Neurobiology and Behavior, Columbia University, New York, NY 10032, USA.
authors.forename: Patrick
authors.initials: P
authors.lastname: Kaifosh
authors.affiliation: Department of Neuroscience, Columbia University, New York, NY 10032, USA; Doctoral Program in Neurobiology and Behavior, Columbia University, New York, NY 10032, USA.
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Zaremba
authors.affiliation: Department of Neuroscience, Columbia University, New York, NY 10032, USA; Department of Bioengineering, Stanford University, Stanford, CA 94305, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Lovett-Barron
authors.affiliation: Department of Neuroscience, Columbia University, New York, NY 10032, USA; Doctoral Program in Neurobiology and Behavior, Columbia University, New York, NY 10032, USA.
authors.forename: Joseph
authors.initials: J
authors.lastname: Tsai
authors.affiliation: Department of Psychiatry, Columbia University, New York, NY 10032, USA; Division of Integrative Neuroscience, New York State Psychiatric Institute, New York, NY 10032, USA.
authors.forename: Christine A
authors.initials: CA
authors.lastname: Denny
authors.affiliation: Department of Neuroscience, Columbia University, New York, NY 10032, USA; Doctoral Program in Neurobiology and Behavior, Columbia University, New York, NY 10032, USA.
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Balough
authors.affiliation: Division of Integrative Neuroscience, New York State Psychiatric Institute, New York, NY 10032, USA.
authors.forename: Alexander R
authors.initials: AR
authors.lastname: Goldberg
authors.affiliation: Department of Psychiatry, Columbia University, New York, NY 10032, USA; Division of Integrative Neuroscience, New York State Psychiatric Institute, New York, NY 10032, USA; Wolfson Institute for Biomedical Research, UCL, London WC1E 0BT, UK.
authors.forename: Liam J
authors.initials: LJ
authors.lastname: Drew
authors.affiliation: Department of Psychiatry, Columbia University, New York, NY 10032, USA; Division of Integrative Neuroscience, New York State Psychiatric Institute, New York, NY 10032, USA; Department of Neuroscience, Columbia University, New York, NY 10032, USA; Kavli Institute for Brain Science, Columbia University, New York, NY 10032, USA.
authors.forename: René
authors.initials: R
authors.lastname: Hen
authors.affiliation: Department of Neuroscience, Columbia University, New York, NY 10032, USA; Kavli Institute for Brain Science, Columbia University, New York, NY 10032, USA. Electronic address: al2856@cumc.columbia.edu.
authors.forename: Attila
authors.initials: A
authors.lastname: Losonczy
authors.affiliation: Department of Psychiatry, Columbia University, New York, NY 10032, USA; Division of Integrative Neuroscience, New York State Psychiatric Institute, New York, NY 10032, USA; Department of Psychiatry, University of California, San Francisco, San Francisco, CA 94158, USA; Center for Integrative Neuroscience, University of California, San Francisco, San Francisco, CA 94158, USA.
authors.forename: Mazen A
authors.initials: MA
authors.lastname: Kheirbek
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002454:Cell Differentiation; D018891:Dentate Gyrus / Q000166:cytology / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton; D055495:Neurogenesis; D009474:Neurons / Q000378:metabolism*; D062308:Optogenetics
chemical_list: D002118:Calcium