title: Hyperbaric Oxygen Therapy Suppresses Apoptosis and Promotes Renal Tubular Regeneration After Renal Ischemia/Reperfusion Injury in Rats.
abstract: BACKGROUND
  Renal ischemia/reperfusion (I/R) injury remains a major cause of acute kidney injury (AKI), in addition to I/R injury-induced tissue inflammation, necrosis and apoptosis. Hyperbaric oxygen therapy (HBO) is defined as a treatment in which a patient is intermittently exposed to 100% oxygen pressurized to a pressure above sea level (> 2.0 atmospheres absolute (ATA), 1.0 ATA = 760 mmHg). It has been used in a number of medical conditions with a proven efficacy in a limited number of disorders. However, the effects of HBO therapy on apoptosis and proliferative activity after I/R injury have not been fully understood.
  
  
  OBJECTIVE
  We studied the possible beneficial effects of HBO therapy on apoptosis and tubular cell regeneration after renal I/R injury in rats.
  
  
  METHODS
  Sprague-Dawley (SD) rats were randomized into three groups: Sham (Sham-operated rats); I/R (animals submitted to I/R); and I/R + HBO (I/R rats exposed to HBO). Tubular cell apoptosis was confirmed by DNA laddering and the terminal deoxynucleotidyl transferase-mediated uridine triphosphate nick end labeling (TUNEL) assay. Cellular proliferation activity was determined using the anti-Ki-67 antibody.
  
  
  RESULTS
  A significant decrease in apoptotic cells and increase in proliferative reaction were observed in the I/R + HBO group compared to the I/R group.
  
  
  CONCLUSIONS
  We demonstrated that HBO suppressed apoptosis, which caused inflammation after renal I/R, and promoted tubular cell regeneration. HBO has protective effects against AKI caused by renal I/R through the inhibition of apoptosis.
authors.affiliation: Department of Medical Engineering, Faculty of Health Sciences, Kyushu University of Health and Welfare, Yoshinomachi, Nobeoka, Japan.
authors.forename: Heihachi
authors.initials: H
authors.lastname: Migita
authors.affiliation: Department of Medical Engineering, Faculty of Health Sciences, Kyushu University of Health and Welfare, Yoshinomachi, Nobeoka, Japan.
authors.forename: Shigenori
authors.initials: S
authors.lastname: Yoshitake
authors.affiliation: Department of Medical Engineering, Faculty of Health Sciences, Kyushu University of Health and Welfare, Yoshinomachi, Nobeoka, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Tange
authors.affiliation: Department of Anatomy, Faculty of Medicine, Division of Histochemistry and Cell Biology, University of Miyazaki, Kyotake, Miyazaki, Japan.
authors.forename: Narantsog
authors.initials: N
authors.lastname: Choijookhuu
authors.affiliation: Department of Anatomy, Faculty of Medicine, Division of Histochemistry and Cell Biology, University of Miyazaki, Kyotake, Miyazaki, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Hishikawa
keywords: Apoptosis; Hyperbaric Oxygen Therapy; Ischemia/Reperfusion Injury; Rats