title: Inhibition of P38 MAPK Downregulates the Expression of IL-1β to Protect Lung from Acute Injury in Intestinal Ischemia Reperfusion Rats.
abstract: Acute lung injury (ALI) induced by intestinal ischemia/reperfusion (II/R) has high incidence and mortality, in which IL-1β was essential for the full development of ALI. However, the detailed regulating mechanism for this phenomenon remains to be unclear. The purpose of this study was to investigate whether inhibition of P38 MAPK could downregulate the expression of IL-1β to protect lung from acute injury in II/R rats. Here, we found that the level of pulmonary edema at 16 hours after operation (hpo) was obviously enhanced compared to that in 8hpo and sham groups. Immunofluorescent staining demonstrated that IL-1β and P38 MAPK were detected in lung tissues. And rats with II/R have the highest translation level for IL-1β and phosphorylation of P38 MAPK in lung tissues at 16hpo compared with 8hpo and sham groups. Moreover, administration of SB239063, an inhibitor of P38 α and β, could effectively downregulate the expressions of IL-1β and protects lung tissues from injury in II/R rats. Our findings indicate that the inhibition of P38 α and β may downregulate the expression of IL-1β to protect lung from acute injury in II/R, which could be used as a potential target for reducing ALI induced by II/R in the future clinical trial.
authors.affiliation: Department of Burn and Plastic Surgery, Guizhou Provincial People's Hospital, Guiyang 550002, China.
authors.forename: De-Yi
authors.initials: DY
authors.lastname: Zheng
authors.affiliation: Institute of Neuroscience, Kunming Medical University, Kunming 650031, China; Institute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Department of Pediatrics, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Jin
authors.affiliation: Institute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Mu
authors.initials: M
authors.lastname: He
authors.affiliation: Department of Burn and Plastic Surgery, Guizhou Provincial People's Hospital, Guiyang 550002, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Burn and Plastic Surgery, Guizhou Provincial People's Hospital, Guiyang 550002, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Du
authors.affiliation: Department of Burn and Plastic Surgery, Guizhou Provincial People's Hospital, Guiyang 550002, China.
authors.forename: Xiang-Yang
authors.initials: XY
authors.lastname: Xiao
authors.affiliation: Department of Burn and Plastic Surgery, Guizhou Provincial People's Hospital, Guiyang 550002, China.
authors.forename: Ping-Yang
authors.initials: PY
authors.lastname: Li
authors.affiliation: Department of Pediatrics, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China.
authors.forename: Ai-Zhu
authors.initials: AZ
authors.lastname: Ye
authors.affiliation: Institute of Neuroscience, Kunming Medical University, Kunming 650031, China; Experimental Animal Center, Kunming Medical University, Kunming 650031, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Neuroscience, Kunming Medical University, Kunming 650031, China; Institute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, China; Experimental Animal Center, Kunming Medical University, Kunming 650031, China.
authors.forename: Ting-Hua
authors.initials: TH
authors.lastname: Wang
mesh_terms: D055371:Acute Lung Injury / Q000378:metabolism* / Q000473:pathology / Q000517:prevention & control; D000818:Animals; D004791:Enzyme Inhibitors / Q000627:therapeutic use; D053583:Interleukin-1beta / Q000378:metabolism*; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008297:Male; D010766:Phosphorylation; D011654:Pulmonary Edema / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D053583:Interleukin-1beta; D048051:p38 Mitogen-Activated Protein Kinases