title: Synthesis of functionalized gold nanoparticles capped with 3-mercapto-1-propansulfonate and 1-thioglucose mixed thiols and "in vitro" bioresponse.
abstract: The synthesis, characterization and assessment of biological behavior of innovative negatively charged functionalized gold nanoparticles is herein reported, for potential applications in the field of radiotherapy and drug delivery. Gold nanoparticles (AuNPs) functionalized with two capping agents, i.e., the 3-mercapto-1-propansulfonate (3-MPS) and 1-β-thio-D-glucose (TG), have been on purpose synthesized and fully characterized. Advanced characterization techniques including X-Ray Photoelectron Spectroscopy (XPS) were applied to probe the chemical structure of the synthesized nanomaterials. Z-potential and Dynamic Light Scattering measurements allowed assessing the nanodimension, dispersity, surface charge and stability of AuNPs. Transmission Electron Microscopy (TEM) and Flame Atomic Absorption Spectroscopy (FAAS) were applied to the "in vitro" HSG cell model, to investigate the nanoparticles-cells interaction and to evaluate the internalization efficiency, whereas short term cytotoxicity and long term cell killing were evaluated by means of MTT and SRB assays, respectively. In conclusion, in order to increase the amount of gold atoms inside the cell we have optimized the synthesis for a new kind of biocompatible and very stable negatively charged TG-functionalized nanoparticles, with diameters in a range that maximize the uptake in cells (i.e., ∼15nm). Such particles are very promising for radiotherapy and drug delivery application.
authors.affiliation: Roma Tre University, Dept. of Sciences, Via della Vasca Navale 79, 00146 Rome Italy.
authors.forename: F
authors.initials: F
authors.lastname: Porcaro
authors.affiliation: Roma Tre University, Dept. of Sciences, Via della Vasca Navale 79, 00146 Rome Italy.
authors.forename: C
authors.initials: C
authors.lastname: Battocchio
authors.affiliation: Roma Tre University, Dept. of Sciences, Via della Vasca Navale 79, 00146 Rome Italy.
authors.forename: A
authors.initials: A
authors.lastname: Antoccia
authors.affiliation: Sapienza University, Dept. of Chemistry, P.le A. Moro 5, 00085 Rome Italy. Electronic address: ilaria.fratoddi@uniroma1.it.
authors.forename: I
authors.initials: I
authors.lastname: Fratoddi
authors.affiliation: Sapienza University, Dept. of Chemistry, P.le A. Moro 5, 00085 Rome Italy.
authors.forename: I
authors.initials: I
authors.lastname: Venditti
authors.affiliation: Roma Tre University, Dept. of Sciences, Via della Vasca Navale 79, 00146 Rome Italy.
authors.forename: A
authors.initials: A
authors.lastname: Fracassi
authors.affiliation: Roma Tre University, Dept. of Sciences, Via della Vasca Navale 79, 00146 Rome Italy.
authors.forename: I
authors.initials: I
authors.lastname: Luisetto
authors.affiliation: Sapienza University, Dept. of Chemistry, P.le A. Moro 5, 00085 Rome Italy.
authors.forename: M V
authors.initials: MV
authors.lastname: Russo
authors.affiliation: Roma Tre University, Dept. of Sciences, Via della Vasca Navale 79, 00146 Rome Italy.
authors.forename: G
authors.initials: G
authors.lastname: Polzonetti
mesh_terms: D001692:Biological Transport; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers; D005947:Glucose / Q000031:analogs & derivatives* / Q000737:chemistry; D006046:Gold / Q000737:chemistry* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D012235:Rhodamines; D055672:Static Electricity; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013499:Surface Properties
chemical_list: C579787:3-mercapto-1-propanesulfonate; D004337:Drug Carriers; D012235:Rhodamines; D013438:Sulfhydryl Compounds; C022027:lissamine rhodamine B; D006046:Gold; C018399:1-thioglucose; D005947:Glucose
keywords: Functionalized gold nanoparticles; Nanoparticles-cells inteaction; Thioglucose