title: Correlation of bioelectrical impedance analysis phase angle with changes in oxidative stress on end-stage renal disease patients, before, during, and after dialysis.
abstract: Chronic kidney disease is a condition that promotes oxidative stress. There are conflicting evidence about the role of hemodialysis on oxidative stress, that are mostly related with the various types of membrane materials used, the quality and type of dialysate, the method used, etc. The phase angle (PhA), which is determined with bioelectrical impedance analysis (BIA), measures the functionality of cell membranes. In this study, the correlation of the PhA with parameters of oxidative stress is attempted for the first time. We evaluated parameters of oxidative status as total antioxidant capacity (TAC) in erythrocytes (RBCs) and plasma of patients with ESRD undergoing hemodialysis with low flux synthetic polysulfone membranes. Measurements were recorded from 30 patients (16 men and 14 women) aged 64 ± 14 years before, during, and after dialysis, and in 15 healthy volunteers aged 56 ± 12 years The PhA was obtained by BIA. The plasma TAC increased significantly (41%, p < 0.05). Intracellular TAC noted a non-significant increase. Total antioxidant capacity of the patients before and after hemodialysis was significantly lower from the healthy volunteers (p < 0.05) showing that ESRD patients are at the state of increased oxidative stress. The PhA increased in significantly positive correlation with plasma TAC at the end of hemodialysis. The process of hemodialysis with biocompatible synthetic membranes and bicarbonate dialysate improved plasma TAC. The positive correlation of PhA with extracellular TAC could evolve to a method of oxidative stress estimation by BIA but further research is needed.
authors.affiliation: a Laboratory of Physiology, Faculty of Medicine , University of Ioannina , Ioannina , Greece ;|b Renal Unit, General Hospital of Filiates , Filiates , Greece ;
authors.forename: Andreas
authors.initials: A
authors.lastname: Zouridakis
authors.affiliation: a Laboratory of Physiology, Faculty of Medicine , University of Ioannina , Ioannina , Greece ;
authors.forename: Yannis V
authors.initials: YV
authors.lastname: Simos
authors.affiliation: a Laboratory of Physiology, Faculty of Medicine , University of Ioannina , Ioannina , Greece ;
authors.forename: Ioannis I
authors.initials: II
authors.lastname: Verginadis
authors.affiliation: c Department of Physiology , Medical School, Democritus University , Alexandroupoli , Greece ;
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Charalabopoulos
authors.affiliation: d Maxillofacial Surgery, Faculty of Medicine , University of Ioannina , Ioannina , Greece ;
authors.forename: Vasilios
authors.initials: V
authors.lastname: Ragos
authors.affiliation: e Department of Nephrology , Medical School, University of Ioannina , Ioannina , Greece.
authors.forename: Evangelia
authors.initials: E
authors.lastname: Dounousi
authors.affiliation: a Laboratory of Physiology, Faculty of Medicine , University of Ioannina , Ioannina , Greece ;
authors.forename: Georgios
authors.initials: G
authors.lastname: Boudouris
authors.affiliation: a Laboratory of Physiology, Faculty of Medicine , University of Ioannina , Ioannina , Greece ;
authors.forename: Spyridon
authors.initials: S
authors.lastname: Karkabounas
authors.affiliation: a Laboratory of Physiology, Faculty of Medicine , University of Ioannina , Ioannina , Greece ;
authors.forename: Angelos
authors.initials: A
authors.lastname: Evangelou
authors.affiliation: a Laboratory of Physiology, Faculty of Medicine , University of Ioannina , Ioannina , Greece ;
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Peschos
mesh_terms: D000368:Aged; D000975:Antioxidants / Q000378:metabolism*; D001672:Biocompatible Materials / Q000494:pharmacology; D015314:Dialysis Solutions / Q000494:pharmacology*; D017097:Electric Impedance; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy; D008297:Male; D008875:Middle Aged; D010043:Outcome and Process Assessment, Health Care; D018384:Oxidative Stress; D011108:Polymers / Q000494:pharmacology*; D006435:Renal Dialysis / Q000009:adverse effects / Q000295:instrumentation / Q000379:methods; D013223:Statistics as Topic; D013450:Sulfones / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D001672:Biocompatible Materials; D015314:Dialysis Solutions; D011108:Polymers; D013450:Sulfones; C017662:polysulfone P 1700
keywords: Antioxidant capacity; dialysis; oxidative stress; phase angle