title: Effects of macroporous hydroxyapatite carriers on the growth and function of human hepatoblasts derived from fetal hepatocytes.
abstract: Improvement of three-dimensional (3D) culture conditions, including substrates for cell growth, is needed for various cell-based applications. In this study, we developed hydroxyapatite (HAp) macroporous carriers having several pore size distributions and tried to obtain the findings about the effective pore sizes for the growth and function of hepatoblasts derived from human fetal hepatocytes. Cellular CYP3A4 activity was significantly enhanced when 20% HAp macroporous carrier was used, reaching 1.49±0.28 pmol/10(6) cells/min of benzyloxyresorufin-O-dealkylation activity, which is comparable to that of primary human hepatocytes from livers of adult donors. Analysis of the pore size (the radius of curvature) distribution of each HAp carrier using a 3D-electron beam surface roughness analyzer revealed two peaks of pore size distribution at 30-40 μm and 70-80 μm, respectively. Thirty-five percent of the pores in the 20% carrier had a size distribution within 50-80 μm. Especially, pores of 70-80 μm were more abundant in the 20% HAp carrier than in the 10% and 30% HAp carriers. These results suggested that a HAp carrier with the pore size distribution of 50-80 μm might be effective for cell growth and function in human hepatoblasts derived from fetal hepatocytes.
authors.affiliation: Division of Applied Life Science, Graduate School of Engineering, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Ishii
authors.affiliation: Division of Applied Life Science, Graduate School of Engineering, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Saito
authors.affiliation: Division of Applied Life Science, Graduate School of Engineering, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Komizu
authors.affiliation: Division of Applied Chemistry, Graduate School of Engineering, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan.
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Tomoshige
authors.affiliation: Division of Applied Life Science, Graduate School of Engineering, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan. Electronic address: matusita@life.sojo-u.ac.jp.
authors.forename: Taku
authors.initials: T
authors.lastname: Matsushita
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017886:Durapatite / Q000737:chemistry* / Q000494:pharmacology*; D005333:Fetus / Q000166:cytology*; D022781:Hepatocytes / Q000166:cytology* / Q000187:drug effects*; D006801:Humans; D008099:Liver / Q000166:cytology; D016062:Porosity
chemical_list: D017886:Durapatite
keywords: CYP3A4; Cell proliferation; Human fetal hepatocytes; Human hepatoblast; Hydroxyapatite; Macroporous carrier; Pore size; Radius of curvature