title: Extracts of Bauhinia championii (Benth.) Benth. inhibit NF-<kappa>B-signaling in a rat model of collagen-induced arthritis and primary synovial cells.
abstract: BACKGROUND
  Bauhinia championii (Benth.) Benth. is used in Chinese traditional medicine to treat arthritis, especially has been used a long time ago on rheumatoid arthritis (RA) in She ethnic minority group.
  
  
  OBJECTIVE
  To investigate the anti-RA effect of Bauhinia championii (Benth.) Benth ethyl acetate extract (BCBEE) and the molecular bases of it.
  
  
  METHODS
  BCBEE was studied on a rat model of RA induced by Ⅱcollagen in vivo, as well as on primary synovial cells in vitro.
  
  
  RESULTS
  After BCBEE treatment, in vivo, it was showed that paw and joint edema was inhibited, pathological joint changes was ameliorated and the levels of interleukin (IL)-1β and tumor necrosis factor-<alpha>(TNF-α) was decreased significantly. The protein and mRNA expressions of nuclear factor-<kappa>B (NF-κB)(p65), IκB, p-IκB and IκB kinase beta (IκKβ) were also down-regulated. Moreover, the in vitro study revealed that BCBEE treatment inhibited primary synovial cells proliferation, and promoted down-regulation of NF-κB(p65), IκB, p-IκB and IκKβ.
  
  
  CONCLUSIONS
  Taken together, the present study demonstrates that BCBEE produces a protection in a rat model of RA induced by Ⅱcollagen via inhibiting paw and joint edema, ameliorating pathological joint changes and regulating the levels of cytokines and its action mechanism maybe is via down-regulating NF-κB(p65), IκB, p-IκB and IκKβ expression.
authors.affiliation: Pharmacy College of Fujian University of Traditional Chinese Medicine, Minhou Shangjie, Fuzhou, Fujian, China. Electronic address: xwfjlab@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.affiliation: Pharmacy College of Fujian University of Traditional Chinese Medicine, Minhou Shangjie, Fuzhou, Fujian, China.
authors.forename: Mingqing
authors.initials: M
authors.lastname: Huang
authors.affiliation: Pharmacy College of Fujian University of Traditional Chinese Medicine, Minhou Shangjie, Fuzhou, Fujian, China.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Pharmacy College of Fujian University of Traditional Chinese Medicine, Minhou Shangjie, Fuzhou, Fujian, China.
authors.forename: Huang
authors.initials: H
authors.lastname: Li
authors.affiliation: Integrative Medicine College of Fujian University of Traditional Chinese Medicine, Minhou Shangjie, Fuzhou, Fujian, China.
authors.forename: Haiyin
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Pharmacy College of Fujian University of Traditional Chinese Medicine, Minhou Shangjie, Fuzhou, Fujian, China.
authors.forename: Lishuang
authors.initials: L
authors.lastname: Yu
authors.affiliation: Pharmacy College of Fujian University of Traditional Chinese Medicine, Minhou Shangjie, Fuzhou, Fujian, China.
authors.forename: Kedan
authors.initials: K
authors.lastname: Chu
mesh_terms: D000818:Animals; D001168:Arthritis / Q000139:chemically induced / Q000188:drug therapy*; D036481:Bauhinia / Q000737:chemistry*; D003094:Collagen / Q000633:toxicity*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects; D013583:Synovial Membrane / Q000187:drug effects*
chemical_list: D016207:Cytokines; D010936:Plant Extracts; D003094:Collagen
keywords: Bauhinia championii (Benth.) Benth.; IκKβ; NF-κB; Primary synovial cells; Rheumatoid arthritis; p-IκB