title: De novo assembly and comparison of the ovarian transcriptomes of the common Chinese cuttlefish (Sepiella japonica) with different gonadal development.
abstract: The common Chinese cuttlefish (Sepiella japonica) has been considered one of the most economically important marine Cephalopod species in East Asia and seed breeding technology has been established for massive aquaculture and stock enhancement. In the present study, we used Illumina HiSeq2000 to sequence, assemble and annotate the transcriptome of the ovary tissues of S. japonica for the first time. A total of 53,116,650 and 53,446,640 reads were obtained from the immature and matured ovaries, respectively (NCBI SRA database SRX1409472 and SRX1409473), and 70,039 contigs (N50 = 1443 bp) were obtained after de novo assembling with Trinity software. Digital gene expression analysis reveals 47,288 contigs show differential expression profile and 793 contigs are highly expressed in the immature ovary, while 38 contigs are highly expressed in the mature ovary with FPKM > 100. We hope that the ovarian transcriptome and those stage-enriched transcripts of S. japonica can provide some insight into the understanding of genome-wide transcriptome profile of cuttlefish gonad tissue and give useful information in cuttlefish gonad development.
authors.affiliation: National Engineering Research Center for Facilitated Marine Aquaculture, Marine Science College, Zhejiang Ocean University, Zhoushan 316004, China.
authors.forename: Zhenming
authors.initials: Z
authors.lastname: Lü
authors.affiliation: National Engineering Research Center for Facilitated Marine Aquaculture, Marine Science College, Zhejiang Ocean University, Zhoushan 316004, China.
authors.forename: Wan
authors.initials: W
authors.lastname: Liu
authors.affiliation: National Engineering Research Center for Facilitated Marine Aquaculture, Marine Science College, Zhejiang Ocean University, Zhoushan 316004, China.
authors.forename: Liqin
authors.initials: L
authors.lastname: Liu
authors.affiliation: Marine Fisheries Research Institute of Zhejiang, Zhoushan 316021, China.
authors.forename: Huilai
authors.initials: H
authors.lastname: Shi
authors.affiliation: Marine Fisheries Research Institute of Zhejiang, Zhoushan 316021, China.
authors.forename: Hongling
authors.initials: H
authors.lastname: Ping
authors.affiliation: National Engineering Research Center for Facilitated Marine Aquaculture, Marine Science College, Zhejiang Ocean University, Zhoushan 316004, China.
authors.forename: Tianming
authors.initials: T
authors.lastname: Wang
authors.affiliation: National Engineering Research Center for Facilitated Marine Aquaculture, Marine Science College, Zhejiang Ocean University, Zhoushan 316004, China.
authors.forename: Changfeng
authors.initials: C
authors.lastname: Chi
authors.affiliation: National Engineering Research Center for Facilitated Marine Aquaculture, Marine Science College, Zhejiang Ocean University, Zhoushan 316004, China.
authors.forename: Changwen
authors.initials: C
authors.lastname: Wu
authors.affiliation: WeThink Biotech Inc., Taoyuan, Taiwan.
authors.forename: Ching-Hung
authors.initials: CH
authors.lastname: Chen
authors.affiliation: Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung 20224, Taiwan.
authors.forename: Kang-Ning
authors.initials: KN
authors.lastname: Shen
authors.affiliation: Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li 32023, Taiwan.
authors.forename: Chung-Der
authors.initials: CD
authors.lastname: Hsiao
keywords: Cuttlefish; Gonad development; Transcriptome