title: Antioxidant and gene expression responses of Eisenia fetida following repeated exposure to BDE209 and Pb in a soil-earthworm system.
abstract: This study first adopted repeated treatment model to investigate stress responses in earthworms (Eisenia fetida) following exposure to decabromodiphenyl ether (BDE209) and lead (Pb), which are the mainly co-existed contaminants at e-waste recycling sites. We evaluated the impacts of BDE209-Pb on antioxidative enzyme (superoxide dismutase, SOD; catalase, CAT) activities, malondialdehyde (MDA) contents and transcriptional levels of three target genes (SOD, CAT and Hsp70), and further explored the relationships among these biomarkers. Results demonstrated that almost all the parameters were generally induced and the responses followed certain dose-effect relationships. Compared to the controls, a significant (P<0.05) up-regulation trend of expression levels of the three genes could be clearly observed after 14days incubation. Additionally, there existed good correlations between target genes expression levels and antioxidant enzyme activities (R>0.64). The observations could provide important information of ecotoxicological effects of BDE209-Pb in a soil-earthworm system as well as the mechanism of antioxidant defense.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China; State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Shuangqing
authors.initials: S
authors.lastname: Hu
authors.affiliation: State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China. Electronic address: wzhang@ecust.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Kuangfei
authors.initials: K
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China. Electronic address: ji@nju.edu.cn.
authors.forename: Rong
authors.initials: R
authors.lastname: Ji
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D059029:Electronic Waste; D015870:Gene Expression; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D007854:Lead; D008315:Malondialdehyde / Q000378:metabolism; D009835:Oligochaeta / Q000502:physiology*; D059027:Recycling; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism
chemical_list: D015415:Biomarkers; D055768:Halogenated Diphenyl Ethers; D012987:Soil; D012989:Soil Pollutants; D007854:Lead; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase
keywords: Antioxidant enzyme; BDE209-Pb stress; Eisenia fetida; Gene transcription; Repeated exposure