title: Food peptidomics of in vitro gastrointestinal digestions of partially purified bovine hemoglobin: low-resolution versus high-resolution LC-MS/MS analyses.
abstract: Consumers and governments have become aware how the daily diet may affect the human health. All proteins from both plant and animal origins are potential sources of a wide range of bioactive peptides and the large majority of those display health-promoting effects. In the meat production food chain, the slaughterhouse blood is an inevitable co-product and, today, the blood proteins remain underexploited despite their bioactive potentiality. Through a comparative food peptidomics approach we illustrate the impact of resolving power, accuracy, sensitivity, and acquisition speed of low-resolution (LR)- and high-resolution (HR)-LC-ESI-MS/MS on the obtained peptide mappings and discuss the limitations of MS-based peptidomics. From in vitro gastrointestinal digestions of partially purified bovine hemoglobin, we have established the peptide maps of each hemoglobin chain. LR technique (normal bore C18 LC-LR-ESI-MS/MS) allows us to identify without ambiguity 75 unique peptides while the HR approach (nano bore C18 LC-HR-ESI-MS/MS) unambiguously identify more than 950 unique peptides (post-translational modifications included). Herein, the food peptidomics approach using the most performant separation methods and mass spectrometers with high-resolution capabilities appears as a promising source of information to assess the health potentiality of proteins.
authors.affiliation: Univ. Lille, Univ. Artois, EA 7394 - ICV - Institut Charles Viollette, Lille, France.
authors.forename: Juliette
authors.initials: J
authors.lastname: Caron
authors.affiliation: Univ. Lille, Univ. Artois, EA 7394 - ICV - Institut Charles Viollette, Lille, France.
authors.forename: Gabrielle
authors.initials: G
authors.lastname: Chataigné
authors.affiliation: Laboratoire PRISM/clic imaging-U 1192 INSERM Protéomique, réponse inflammatoire et spectrométrie de masse, Université Lille 1, Villeneuve d'Ascq, France.
authors.forename: Jean-Pascal
authors.initials: JP
authors.lastname: Gimeno
authors.affiliation: CUMA, Faculté de Pharmacie, Lille, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Duhal
authors.affiliation: CUMA, Faculté de Pharmacie, Lille, France.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Goossens
authors.affiliation: Univ. Lille, Univ. Artois, EA 7394 - ICV - Institut Charles Viollette, Lille, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Dhulster
authors.affiliation: Univ. Lille, Univ. Artois, EA 7394 - ICV - Institut Charles Viollette, Lille, France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Cudennec
authors.affiliation: Univ. Lille, Univ. Artois, EA 7394 - ICV - Institut Charles Viollette, Lille, France.
authors.forename: Rozenn
authors.initials: R
authors.lastname: Ravallec
authors.affiliation: Univ. Lille, Univ. Artois, EA 7394 - ICV - Institut Charles Viollette, Lille, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Flahaut
mesh_terms: D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D004063:Digestion; D005504:Food Analysis; D006454:Hemoglobins / Q000378:metabolism*; D066298:In Vitro Techniques; D010449:Peptide Mapping; D010455:Peptides / Q000378:metabolism*; D040901:Proteomics; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D006454:Hemoglobins; D010455:Peptides
keywords: Foodomics; Gastrointestinal digestion; NanoLC-HR-ESI-MS/MS; Peptidomics