title: Antisense non-coding RNAs and regulation of gene transcription.
abstract: Transcriptome analyses have revealed the existence of a large variety of non-coding RNAs (ncRNAs) that, although specifically expressed, are largely unknown in function. The best-studied role of ncRNAs is the regulation of gene expression, mostly participating in transcription. We report here the role of an antisense ncRNA that represses transcription of LEF1 by recruiting Polycomb repressive complex 2 (PRC2), which trimethylates H3K27. This antisense LEF1 transcript undergoes splicing losing its inhibitory function. We also discuss the possible general relevance of this finding.
authors.affiliation: a UCL Cancer Institute, University College of London , London , United Kingdom.
authors.forename: Manuel
authors.initials: M
authors.lastname: Beltran
authors.affiliation: b Programa de Recerca en Càncer, Institut Hospital del Mar d'Investigacions Mèdiques (IMIM) , Barcelona , Spain.|c Departament de Ciències Experimentals i de la Salut , Universitat Pompeu Fabra , Barcelona , Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: García de Herreros
mesh_terms: D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000235:genetics*; D056484:Jumonji Domain-Containing Histone Demethylases / Q000378:metabolism; D051821:Lymphoid Enhancer-Binding Factor 1 / Q000235:genetics; D008745:Methylation; D063151:Polycomb Repressive Complex 2 / Q000235:genetics; D012326:RNA Splicing; D022661:RNA, Untranslated / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D051821:Lymphoid Enhancer-Binding Factor 1; D022661:RNA, Untranslated; D056484:Jumonji Domain-Containing Histone Demethylases; C523761:Kdm6b protein, mouse; D063151:Polycomb Repressive Complex 2
keywords: antisense transcription; Epithelial-to-Mesenchymal transition; LEF1 gene; Polycomb repressive complex 2