title: Neurotrophin-4 in the brain of adult Nothobranchius furzeri.
abstract: Neurotrophin-4 (NT-4) is a member of the well-known family of neurotrophins that regulate the development of neuronal networks by participating in neuronal survival and differentiation, the growth of neuronal processes, synaptic development and plasticity, as well as myelination. NT-4 interacts with two distinct receptors: TrkB, high affinity receptor and p75 low-affinity neurotrophin receptor (p75(NTR)). In the present survey, we identified the gene encoding NT-4 in the teleost Nothobranchius furzeri, a model species for aging research. The identified gene shows a similarity of about 72% with medaka, the closest related species. The neuroanatomical localization of NT-4 mRNA is obtained by using an LNA probe. NT-4 mRNA expression is observed in neurons and glial cells of the forebrain and hindbrain, with very low signal found in the midbrain. This survey confirms that NT-4 is expressed in the brain of N. furzeri during adulthood, suggesting that it could also be implicated in the maintenance and regulation of neuronal functions.
authors.affiliation: Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy.
authors.forename: L
authors.initials: L
authors.lastname: D'Angelo
authors.affiliation: Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Avallone
authors.affiliation: Scuola Normale Superiore di Pisa, Pisa, Italy; Laboratory of Aging, Leibniz Institute on Aging - Fritz Lipmann Institute, Jena, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Cellerino
authors.affiliation: Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy.
authors.forename: P
authors.initials: P
authors.lastname: de Girolamo
authors.affiliation: Department of Sciences and Technologies, University of Sannio, Benevento, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Paolucci
authors.affiliation: Department of Sciences and Technologies, University of Sannio, Benevento, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Varricchio
authors.affiliation: Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Lucini
mesh_terms: D000375:Aging / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D023781:Fundulidae / Q000033:anatomy & histology / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000378:metabolism*; D009928:Organ Specificity / Q000502:physiology; D014018:Tissue Distribution
chemical_list: D009414:Nerve Growth Factors; C073922:neurotrophin 4
keywords: Central nervous system, LNA probe; In situ hybridization; Neurotrophin 4