title: An easily regenerable enzyme reactor prepared from polymerized high internal phase emulsions.
abstract: A large-scale high-efficient enzyme reactor based on polymerized high internal phase emulsion monolith (polyHIPE) was prepared. First, a porous cross-linked polyHIPE monolith was prepared by in-situ thermal polymerization of a high internal phase emulsion containing styrene, divinylbenzene and polyglutaraldehyde. The enzyme of TPCK-Trypsin was then immobilized on the monolithic polyHIPE. The performance of the resultant enzyme reactor was assessed according to the conversion ability of Nα-benzoyl-l-arginine ethyl ester to Nα-benzoyl-l-arginine, and the protein digestibility of bovine serum albumin (BSA) and cytochrome (Cyt-C). The results showed that the prepared enzyme reactor exhibited high enzyme immobilization efficiency and fast and easy-control protein digestibility. BSA and Cyt-C could be digested in 10 min with sequence coverage of 59% and 78%, respectively. The peptides and residual protein could be easily rinsed out from reactor and the reactor could be regenerated easily with 4 M HCl without any structure destruction. Properties of multiple interconnected chambers with good permeability, fast digestion facility and easily reproducibility indicated that the polyHIPE enzyme reactor was a good selector potentially applied in proteomics and catalysis areas.
authors.affiliation: Guangxi Key Laboratory of Electrochemical and Magnetochemical Functional Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi 541004, China; Guangxi Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area, Guilin University of Technology, Guilin 541004, China. Electronic address: guihuaruan@hotmail.com.
authors.forename: Guihua
authors.initials: G
authors.lastname: Ruan
authors.affiliation: Guangxi Key Laboratory of Electrochemical and Magnetochemical Functional Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi 541004, China.
authors.forename: Zhenwei
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Guangxi Key Laboratory of Electrochemical and Magnetochemical Functional Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi 541004, China.
authors.forename: Yipeng
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Guangxi Key Laboratory of Electrochemical and Magnetochemical Functional Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi 541004, China.
authors.forename: Meiping
authors.initials: M
authors.lastname: Wei
authors.affiliation: Guangxi Key Laboratory of Electrochemical and Magnetochemical Functional Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi 541004, China.
authors.forename: Rihui
authors.initials: R
authors.lastname: Su
authors.affiliation: Guangxi Key Laboratory of Electrochemical and Magnetochemical Functional Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi 541004, China; Guangxi Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area, Guilin University of Technology, Guilin 541004, China. Electronic address: dufu2005@126.com.
authors.forename: Fuyou
authors.initials: F
authors.lastname: Du
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents / Q000737:chemistry; D003580:Cytochromes / Q000737:chemistry; D004655:Emulsions; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005976:Glutaral / Q000031:analogs & derivatives / Q000737:chemistry; D006851:Hydrochloric Acid / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D058105:Polymerization; D011108:Polymers / Q000737:chemistry; D011312:Pressure; D011506:Proteins / Q000737:chemistry; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013343:Styrenes / Q000737:chemistry; D053719:Tandem Mass Spectrometry; D014357:Trypsin / Q000737:chemistry; D014753:Vinyl Compounds / Q000737:chemistry
chemical_list: D003432:Cross-Linking Reagents; D003580:Cytochromes; D004655:Emulsions; D004800:Enzymes, Immobilized; D011108:Polymers; D011506:Proteins; D013343:Styrenes; D014753:Vinyl Compounds; C493734:polyHIPE; D012710:Serum Albumin, Bovine; C022841:poly(glutaraldehyde); D014357:Trypsin; C004985:divinyl benzene; D006851:Hydrochloric Acid; D005976:Glutaral
keywords: High internal phase emulsion; Immobilized enzymatic reactor; PolyHIPE monoliths; Protein digestion