title: Calcium imaging of neural circuits with extended depth-of-field light-sheet microscopy.
abstract: Increasing the volumetric imaging speed of light-sheet microscopy will improve its ability to detect fast changes in neural activity. Here, a system is introduced for brain-wide imaging of neural activity in the larval zebrafish by coupling structured illumination with cubic phase extended depth-of-field (EDoF) pupil encoding. This microscope enables faster light-sheet imaging and facilitates arbitrary plane scanning-removing constraints on acquisition speed, alignment tolerances, and physical motion near the sample. The usefulness of this method is demonstrated by performing multi-plane calcium imaging in the fish brain with a 416×832×160  μm field of view at 33 Hz. The optomotor response behavior of the zebrafish is monitored at high speeds, and time-locked correlations of neuronal activity are resolved across its brain.
authors.forename: Sean
authors.initials: S
authors.lastname: Quirin
authors.forename: Nikita
authors.initials: N
authors.lastname: Vladimirov
authors.forename: Chao-Tsung
authors.initials: CT
authors.lastname: Yang
authors.forename: Darcy S
authors.initials: DS
authors.lastname: Peterka
authors.forename: Rafael
authors.initials: R
authors.lastname: Yuste
authors.forename: Misha B
authors.initials: MB
authors.lastname: Ahrens
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology; D017173:Caenorhabditis elegans; D002118:Calcium / Q000378:metabolism*; D008027:Light; D008853:Microscopy / Q000379:methods*; D057054:Molecular Imaging; D009474:Neurons / Q000378:metabolism*
chemical_list: D002118:Calcium