title: Alpinate Oxyphyllae Fructus Inhibits IGFII-Related Signaling Pathway to Attenuate Ang II-Induced Pathological Hypertrophy in H9c2 Cardiomyoblasts.
abstract: Angiotensin II (Ang II) is a very important cardiovascular disease inducer and may cause cardiac pathological hypertrophy and remodeling. We evaluated a Chinese traditional medicine, alpinate oxyphyllae fructus (AOF), for therapeutic efficacy for treating Ang II-induced cardiac hypertrophy. AOF has been used to treat patients with various symptoms accompanying hypertension and cerebrovascular disorders in Korea. We investigated its protective effect against Ang II-induced cytoskeletal change and hypertrophy in H9c2 cells. The results showed that treating cells with Ang II resulted in pathological hypertrophy, such as increased expression of transcription factors NFAT-3/p-NFAT-3, hypertrophic response genes (atrial natriuretic peptide [ANP] and b-type natriuretic peptide [BNP]), and Gαq down-stream effectors (PLCβ3 and calcineurin). Pretreatment with AOF (60-100 μg/mL) led to significantly reduced hypertrophy. We also found that AOF pretreatment significantly suppressed the cardiac remodeling proteins, metalloproteinase (MMP9 and MMP2), and tissue plasminogen activator (tPA), induced by Ang II challenge. In conclusion, we provide evidence that AOF protects against Ang II-induced pathological hypertrophy by specifically inhibiting the insulin-like growth factor (IGF) II/IIR-related signaling pathway in H9c2 cells. AOF might be a candidate for cardiac hypertrophy and ventricular remodeling prevention in chronic cardiovascular diseases.
authors.affiliation: 1 1PT Biotechnology Co., Ltd. , Taichung, Taiwan .
authors.forename: Chuan-Te
authors.initials: CT
authors.lastname: Tsai
authors.affiliation: 1 1PT Biotechnology Co., Ltd. , Taichung, Taiwan .|2 The School of Chinese Medicine for Post-Baccalaureate, I-Shou University , Kaohsiung, Taiwan .|3 Chinese Medicine Department, E-DA Hospital , Kaohsiung, Taiwan .
authors.forename: Yung-Ming
authors.initials: YM
authors.lastname: Chang
authors.affiliation: 4 1PT Lukang Chinese Medicine Clinics , Changhua, Taiwan .
authors.forename: Shu-Luan
authors.initials: SL
authors.lastname: Lin
authors.affiliation: 5 School of Chinese Medicine, China Medical University , Taichung, Taiwan .
authors.forename: Yueh-Sheng
authors.initials: YS
authors.lastname: Chen
authors.affiliation: 6 Department of pathology, Changhua Christian Hospital , Changhua, Taiwan .|7 Department of Medical Technology, Jen-Teh Junior College of Medicine , Nursing and Management, Miaoli, Taiwan .
authors.forename: Yu-Lan
authors.initials: YL
authors.lastname: Yeh
authors.affiliation: 8 Department of Biotechnology, Bharathiar University , Coimbatore, India .
authors.forename: Viswanadha Vijaya
authors.initials: VV
authors.lastname: Padma
authors.affiliation: 2 The School of Chinese Medicine for Post-Baccalaureate, I-Shou University , Kaohsiung, Taiwan .|3 Chinese Medicine Department, E-DA Hospital , Kaohsiung, Taiwan .
authors.forename: Chin-Chuan
authors.initials: CC
authors.lastname: Tsai
authors.affiliation: 9 Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University , Taipei, Taiwan .
authors.forename: Ray-Jade
authors.initials: RJ
authors.lastname: Chen
authors.affiliation: 5 School of Chinese Medicine, China Medical University , Taichung, Taiwan .
authors.forename: Tsung-Jung
authors.initials: TJ
authors.lastname: Ho
authors.affiliation: 5 School of Chinese Medicine, China Medical University , Taichung, Taiwan .|10 Graduate Institute of Basic Medical Science, China Medical University , Taichung, Taiwan .|11 Department of Health and Nutrition Biotechnology, Asia University , Taichung, Taiwan .
authors.forename: Chih-Yang
authors.initials: CY
authors.lastname: Huang
mesh_terms: D032429:Alpinia / Q000737:chemistry*; D000804:Angiotensin II / Q000009:adverse effects / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D006801:Humans; D006984:Hypertrophy / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D007335:Insulin-Like Growth Factor II / Q000235:genetics / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects
chemical_list: C442779:Alpinia oxyphylla fruit extract; D010936:Plant Extracts; D000804:Angiotensin II; D007335:Insulin-Like Growth Factor II
keywords: H9c2 Cell; alpinate oxyphyllae fructus; angiotensin II; hypertrophy