title: Antibacterial effects of electrospun chitosan/poly(ethylene oxide) nanofibrous membranes loaded with chlorhexidine and silver.
abstract: To prevent percutaneous device associated infections (PDAIs), we prepared electrospun chitosan/poly(ethylene oxide) (PEO) nanofibrous membrane containing silver nanoparticles as an implantable delivery vehicle for the dual release of chlorhexidine and silver ions. We observed that the silver nanoparticles were distributed homogeneously throughout the fibers, and a fast release of chlorhexidine in 2days and a sustained release of silver ions for up to 28days. The antibacterial efficacy of the membranes against Staphylococcus aureus showed that the membranes exhibited an obvious inhibition zone upon loading with either chlorhexidine (20μg or more per membrane) or AgNO3 (1 and 5wt% to polymer). Furthermore, long-term antibacterial effect up to 4days was verified using membranes containing 5wt% AgNO3. The results suggest that the membranes have strong potential to act as an active antibacterial dressing for local delivery of antibacterial agents to prevent PDAIs.
authors.affiliation: Department of Biomaterials, Radboud University Medical Centre, Nijmegen, the Netherlands.
authors.forename: Jiankang
authors.initials: J
authors.lastname: Song
authors.affiliation: Department of Biomaterials, Radboud University Medical Centre, Nijmegen, the Netherlands.
authors.forename: Stefan J A
authors.initials: SJ
authors.lastname: Remmers
authors.affiliation: Department of Biomaterials, Radboud University Medical Centre, Nijmegen, the Netherlands.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Shao
authors.affiliation: Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, the Netherlands.
authors.forename: Eva
authors.initials: E
authors.lastname: Kolwijck
authors.affiliation: Department of Biomaterials, Radboud University Medical Centre, Nijmegen, the Netherlands.
authors.forename: X Frank
authors.initials: XF
authors.lastname: Walboomers
authors.affiliation: Department of Biomaterials, Radboud University Medical Centre, Nijmegen, the Netherlands.
authors.forename: John A
authors.initials: JA
authors.lastname: Jansen
authors.affiliation: Department of Biomaterials, Radboud University Medical Centre, Nijmegen, the Netherlands.
authors.forename: Sander C G
authors.initials: SC
authors.lastname: Leeuwenburgh
authors.affiliation: Department of Biomaterials, Radboud University Medical Centre, Nijmegen, the Netherlands. Electronic address: fang.yang@radboudumc.nl.
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D048271:Chitosan / Q000494:pharmacology*; D002710:Chlorhexidine / Q000494:pharmacology*; D005027:Ethylene Oxide; D007239:Infections; D057139:Nanofibers; D011092:Polyethylene Glycols; D012834:Silver / Q000494:pharmacology*; D013211:Staphylococcus aureus
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver; D011092:Polyethylene Glycols; D048271:Chitosan; D005027:Ethylene Oxide; D002710:Chlorhexidine
keywords: Chitosan; Chlorhexidine; Controlled release; Electrospinning; Silver nanoparticles