title: Inverse correlation between reactive oxygen species in unwashed semen and sperm motion parameters as measured by a computer-assisted semen analyzer.
abstract: This study investigated the correlation between sperm motion parameters obtained by a computer-assisted semen analyzer and levels of reactive oxygen species in unwashed semen. In total, 847 patients, except for azoospermic patients were investigated. At the time of each patient's first consultation, semen parameters were measured using SMAS™ or CellSoft 3000™, and production of reactive oxygen species was measured using a computer-driven LKB Wallac Luminometer 1251 Analyzer. The patients were divided into two groups: reactive oxygen species - positive and negative. The semen parameters within each group were measured using one of the two computer-assisted semen analyzer systems and then compared. Correlations between reactive oxygen species levels and sperm motion parameters in semen from the reactive oxygen species - positive group were also investigated. Reactive oxygen species were detected in semen samples of 282 cases (33.3%). Sperm concentration (P < 0.01; P < 0.01), motility (P < 0.01; P < 0.05), and progressive motility (P < 0.01; P < 0.01) were markedly lower in the reactive oxygen species - positive group than in the reactive oxygen species - negative group. Among the sperm motion parameters in the reactive oxygen species - positive group, sperm concentration (P < 0.01; P < 0.01), motility (P < 0.05; P < 0.01), mALH (P < 0.05; P < 0.01), and progressive motility (P < 0.05; P < 0.01) also showed inverse correlations with the logarithmic transformed reactive oxygen species levels. Therefore, this study demonstrated that excessive reactive oxygen species in semen damage sperm concentration, motility, and other sperm motion parameters.
authors.affiliation: Department of Urology, Reproductive Center, Yokohama City University Medical Center, Yokohama, Kanagawa, Japan.
authors.forename: Teppei
authors.initials: T
authors.lastname: Takeshima
authors.affiliation: Department of Urology, Reproductive Center, Yokohama City University Medical Center, Yokohama, Kanagawa, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Yumura
authors.affiliation: Department of Urology, Reproductive Center, Yokohama City University Medical Center, Yokohama, Kanagawa, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Yasuda
authors.affiliation: Department of Urology, Reproductive Center, Yokohama City University Medical Center, Yokohama, Kanagawa, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sanjo
authors.affiliation: Department of Urology, Reproductive Center, Yokohama City University Medical Center, Yokohama, Kanagawa, Japan.
authors.forename: Shinnosuke
authors.initials: S
authors.lastname: Kuroda
authors.affiliation: Department of Urology, Reproductive Center, Yokohama City University Medical Center, Yokohama, Kanagawa, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yamanaka
authors.affiliation: Department of Urology, Reproductive Center, Yokohama City University Medical Center, Yokohama, Kanagawa, Japan.|YMRAC Urology Office, Yokohama, Kanagawa, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Iwasaki
mesh_terms: D000328:Adult; D000368:Aged; D003936:Diagnosis, Computer-Assisted; D006801:Humans; D007248:Infertility, Male; D049449:Luminescence; D008297:Male; D008875:Middle Aged; D017382:Reactive Oxygen Species / Q000032:analysis*; D012661:Semen / Q000737:chemistry*; D055101:Semen Analysis; D013081:Sperm Motility / Q000502:physiology*; D055815:Young Adult
chemical_list: D017382:Reactive Oxygen Species