title: Polydimethyl siloxane based nanocomposites with antibiofilm properties for biomedical applications.
abstract: Polydimethyl siloxane (PDMS) is an excellent implant material for biomedical applications, but often fails as it is prone to microbial colonization which forms biofilms. In the present study CuO, CTAB capped CuO, and ZnO nanoparticles were tested as nanofillers to enhance the antibiofilm property of PDMS against Staphylococcus aureus and Escherichia coli. In general S. aurues (Gram positive and more hydrophobic) favor PDMS surface than glass while E. coli (Gram negative and more hydrophilic) behaves in a reverse way. Incorporation of nanofillers renders the PDMS surface antibacterial and reduces the attachment of both bacteria. These surfaces are also not cytotoxic nor show any cell damage. Contact angle of the material and the cell surface hydrophobicity influenced the extent of bacterial attachment. Cell viability in biofilms was dependent on the antimicrobial property of the nanoparticles incorporated in the PDMS matrix. Simple regression relationships were able to predict the bacterial attachment and number of dead cells on these nanocomposites. Among the nanocomposites tested, PDMS incorporated with CTAB (cetyl trimethylammonium bromide)-capped CuO appears to be the best antibacterial material with good cyto-compatibility. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1075-1082, 2017.
authors.affiliation: Bioengineering and Drug Design Lab, Department of Biotechnology, Indian Institute of Technology, Chennai, India.|Biofouling and Biofilm Processes Section, Bhabha Atomic Research Centre, Kalpakkam, India.
authors.forename: G Gomathi
authors.initials: GG
authors.lastname: Sankar
authors.affiliation: Biofouling and Biofilm Processes Section, Bhabha Atomic Research Centre, Kalpakkam, India.
authors.forename: P Sriyutha
authors.initials: PS
authors.lastname: Murthy
authors.affiliation: Surface and Nanoscience Division, Indira Gandhi Centre for Atomic Research, Kalpakkam, India.
authors.forename: Arindam
authors.initials: A
authors.lastname: Das
authors.affiliation: Bioengineering and Drug Design Lab, Department of Biotechnology, Indian Institute of Technology, Chennai, India.|Biofouling and Biofilm Processes Section, Bhabha Atomic Research Centre, Kalpakkam, India.
authors.forename: S
authors.initials: S
authors.lastname: Sathya
authors.affiliation: Bioengineering and Drug Design Lab, Department of Biotechnology, Indian Institute of Technology, Chennai, India.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Nankar
authors.affiliation: Biofouling and Biofilm Processes Section, Bhabha Atomic Research Centre, Kalpakkam, India.
authors.forename: V P
authors.initials: VP
authors.lastname: Venugopalan
authors.affiliation: Bioengineering and Drug Design Lab, Department of Biotechnology, Indian Institute of Technology, Chennai, India.
authors.forename: Mukesh
authors.initials: M
authors.lastname: Doble
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000254:growth & development*; D002460:Cell Line; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry / Q000494:pharmacology; D003300:Copper / Q000737:chemistry / Q000494:pharmacology; D004129:Dimethylpolysiloxanes / Q000737:chemistry / Q000494:pharmacology; D004926:Escherichia coli / Q000502:physiology*; D053761:Nanocomposites / Q000737:chemistry*; D051381:Rats; D013211:Staphylococcus aureus / Q000502:physiology*
chemical_list: D002593:Cetrimonium Compounds; D004129:Dimethylpolysiloxanes; C013830:baysilon; D003300:Copper; C030973:cupric oxide; D000077286:Cetrimonium
keywords: E. coli; S. aureus; biofilm; nanocomposites; polydimethyl siloxane