title: A wearable biochemical sensor for monitoring alcohol consumption lifestyle through Ethyl glucuronide (EtG) detection in human sweat.
abstract: We demonstrate for the first time a wearable biochemical sensor for monitoring alcohol consumption through the detection and quantification of a metabolite of ethanol, ethyl glucuronide (EtG). We designed and fabricated two co-planar sensors with gold and zinc oxide as sensing electrodes. We also designed a LED based reporting for the presence of EtG in the human sweat samples. The sensor functions on affinity based immunoassay principles whereby monoclonal antibodies for EtG were immobilized on the electrodes using thiol based chemistry. Detection of EtG from human sweat was achieved through chemiresistive sensing mechanism. In this method, an AC voltage was applied across the two coplanar electrodes and the impedance across the sensor electrodes was measured and calibrated for physiologically relevant doses of EtG in human sweat. EtG detection over a dose concentration of 0.001-100 μg/L was demonstrated on both glass and polyimide substrates. Detection sensitivity was lower at 1 μg/L with gold electrodes as compared to ZnO, which had detection sensitivity of 0.001 μg/L. Based on the detection range the wearable sensor has the ability to detect alcohol consumption of up to 11 standard drinks in the US over a period of 4 to 9 hours.
authors.affiliation: Department of Bioengineering, 800 W. Campbell Rd, University of Texas at Dallas, TX 75080.|EnLiSense LLC, 1813 Audubon Pond way, Allen, TX 75013, USA.
authors.forename: Anjan Panneer
authors.initials: AP
authors.lastname: Selvam
authors.affiliation: EnLiSense LLC, 1813 Audubon Pond way, Allen, TX 75013, USA.
authors.forename: Sriram
authors.initials: S
authors.lastname: Muthukumar
authors.affiliation: Department of Bioengineering, 800 W. Campbell Rd, University of Texas at Dallas, TX 75080.
authors.forename: Vikramshankar
authors.initials: V
authors.lastname: Kamakoti
authors.affiliation: Department of Bioengineering, 800 W. Campbell Rd, University of Texas at Dallas, TX 75080.
authors.forename: Shalini
authors.initials: S
authors.lastname: Prasad
mesh_terms: D000428:Alcohol Drinking / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation; D019719:Diagnostic Equipment; D016903:Drug Monitoring / Q000295:instrumentation*; D017097:Electric Impedance; D000431:Ethanol / Q000378:metabolism / Q000493:pharmacokinetics; D005965:Glucuronates / Q000378:metabolism*; D006801:Humans; D008019:Life Style; D013542:Sweat / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D005965:Glucuronates; C093924:ethyl glucuronide; D000431:Ethanol