title: Highly specific quantification of microRNA by coupling probe-rolling circle amplification and Förster resonance energy transfer.
abstract: MicroRNA (miRNA) plays vital roles in various biological processes. In general, sensitivity and specificity are the major parameters for the quantification of miRNA. In this study, padlock probe-rolling circle amplification and Förster resonance energy transfer (pRCA-FRET) were coupled for specific and quantitative detection of miRNA. pRCA-FRET showed superior specificity to differentiate single-base mismatch and excellent sensitivity with a detection limit of 103 aM. The current method has the potential to quantify low amounts of miRNA in the same family for studies on their biological functions.
authors.affiliation: State Key Laboratory of Natural Medicines and Laboratory of Chemical Biology, China Pharmaceutical University, Nanjing, Jiangsu Province, 210009, People's Republic of China.
authors.forename: Xuri
authors.initials: X
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Natural Medicines and Laboratory of Chemical Biology, China Pharmaceutical University, Nanjing, Jiangsu Province, 210009, People's Republic of China.
authors.forename: Shenrong
authors.initials: S
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Natural Medicines and Laboratory of Chemical Biology, China Pharmaceutical University, Nanjing, Jiangsu Province, 210009, People's Republic of China.
authors.forename: Peiyu
authors.initials: P
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Natural Medicines and Laboratory of Chemical Biology, China Pharmaceutical University, Nanjing, Jiangsu Province, 210009, People's Republic of China. Electronic address: yjchen@cpu.edu.cn.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Chen
mesh_terms: D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D035683:MicroRNAs / Q000032:analysis*; D021141:Nucleic Acid Amplification Techniques
chemical_list: D035683:MicroRNAs
keywords: FRET probe; Förster resonance energy transfer (FRET); MicroRNA; Padlock probe; Rolling circle amplification (RCA)