title: Suppressing H19 Modulates Tumorigenicity and Stemness in U251 and U87MG Glioma Cells.
abstract: Glioblastoma multiforme (GBM) is a type of malignant carcinoma found in the brain. Its high frequency of occurrence and poor survival rate have garnered much research attention in recent years. Long non-coding RNAs (lncRNAs) are known to be related to the formation and progression of several cancer types by both promoting and suppressing tumor transformation. H19 is one such lncRNA and has been shown to be upregulated in a few types of cancer. In this study, we discovered that the expression of H19 increased in GBM cell lines. H19 knocked down GBM cells also displayed decreased cellular proliferation and a higher apoptosis rate when induced by temozolomide. Interestingly, the GBM cell lines U87MG and U251 were found to express cancer stem cell markers CD133, NANOG, Oct4 and Sox2. Expression of these markers was downregulated in H19-deficient cells. Collectively, these data suggest a role for H19 in contributing to GBM malignancy and the maintenance of its stem cell properties.
authors.affiliation: Neurosurgery Department, Qilu Hospital, Shandong University, 107 Wenhua West Road, Lixia District, Jinan, 250012, Shandong, China. leeweiguo_777@126.com.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Li
authors.affiliation: Yantai Yuhuangding Hospital, Yantai, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Jiang
authors.affiliation: Yantai Yuhuangding Hospital, Yantai, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Sun
authors.affiliation: Neurosurgery Department, Qilu Hospital, Shandong University, 107 Wenhua West Road, Lixia District, Jinan, 250012, Shandong, China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Xu
authors.affiliation: Neurosurgery Department, Qilu Hospital, Shandong University, 107 Wenhua West Road, Lixia District, Jinan, 250012, Shandong, China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Ma
authors.affiliation: Neurosurgery Department of No. 3 Hospital of Jinan, Jinan, China.
authors.forename: Rucai
authors.initials: R
authors.lastname: Zhan
mesh_terms: D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D014408:Biomarkers, Tumor / Q000378:metabolism; D063646:Carcinogenesis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003606:Dacarbazine / Q000031:analogs & derivatives / Q000494:pharmacology; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D005909:Glioblastoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D014411:Neoplastic Stem Cells; D062085:RNA, Long Noncoding / Q000235:genetics*; D012334:RNA, Neoplasm / Q000235:genetics*; D000077204:Temozolomide; D015854:Up-Regulation
chemical_list: D018906:Antineoplastic Agents, Alkylating; D014408:Biomarkers, Tumor; D062085:RNA, Long Noncoding; D012334:RNA, Neoplasm; D003606:Dacarbazine; D000077204:Temozolomide
keywords: Glioblastoma; Glioma stem-like cells; H19; LncRNA