title: IL-22 regulation of functional gene expression in salivary gland cells.
abstract: TH17 cells and their associated signature cytokines, IL-17 and IL-22, are highly elevated in primary Sjögren's syndrome (pSjS). The levels of IL-22 present in sera showed significant correlations with many disease parameters, specifically hyposalivation, anti-SSB, anti-SSA/SSB, hypergammaglobulinemia and rheumatoid factor. The present study aims to examine the biological function of IL-22 on human salivary glands. To accomplish the goal, microarray analysis using the HumanHT-12 v4 Expression BeadChip was utilized to determine the biological function of IL-22. Differential expression analyses were conducted using the LIMMA package from the Bioconductor project. MTT assay, flow cytometry and Western blotting were used to identify the function of IL-22 on human salivary gland cells. Results indicate an extensive effect of IL-22 on many major molecular functions including activation of antimicrobial genes and downregulation of immune-associated pathways. Functional studies performed in-vitro using human salivary gland cells treated with IL-22 indicated a direct effect of IL-22 on cell cycling, specifically reducing cellular proliferation at the G2-M phase by activation of STAT3. These results suggest the important role of IL-22 in the salivary gland function. The present study suggests that IL-22 might be involved in regulating inflammation and controlling the cell proliferation in SjS.
authors.affiliation: Department of Infectious Diseases and Pathology, University of Florida College of Veterinary Medicine, 2015 SW 16th Ave, Gainesville, FL 32611, USA.
authors.forename: Tegan N
authors.initials: TN
authors.lastname: Lavoie
authors.affiliation: Department of Infectious Diseases and Pathology, University of Florida College of Veterinary Medicine, 2015 SW 16th Ave, Gainesville, FL 32611, USA.
authors.forename: Wendy C
authors.initials: WC
authors.lastname: Carcamo
authors.affiliation: Department of Infectious Diseases and Pathology, University of Florida College of Veterinary Medicine, 2015 SW 16th Ave, Gainesville, FL 32611, USA.
authors.forename: Arun
authors.initials: A
authors.lastname: Wanchoo
authors.affiliation: Center for Biotechnology and Genomic Medicine, Georgia Health Sciences University, Augusta, GA 30912, USA.
authors.forename: Ashok
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Department of Infectious Diseases and Pathology, University of Florida College of Veterinary Medicine, 2015 SW 16th Ave, Gainesville, FL 32611, USA.
authors.forename: Afife
authors.initials: A
authors.lastname: Gulec
authors.affiliation: Department of Oral and Maxillofacial Diagnostic Sciences, University of Florida College of Dentistry, 1600 SW Archer Rd, Gainesville, FL 32610, USA; Center for Orphan Autoimmune Disorders, University of Florida College of Dentistry, 1600 SW Archer Rd, Gainesville, FL 32610, USA.
authors.forename: Kathleen M
authors.initials: KM
authors.lastname: Berg
authors.affiliation: Department of Oral and Maxillofacial Diagnostic Sciences, University of Florida College of Dentistry, 1600 SW Archer Rd, Gainesville, FL 32610, USA; Center for Orphan Autoimmune Disorders, University of Florida College of Dentistry, 1600 SW Archer Rd, Gainesville, FL 32610, USA.
authors.forename: Carol M
authors.initials: CM
authors.lastname: Stewart
authors.affiliation: Department of Infectious Diseases and Pathology, University of Florida College of Veterinary Medicine, 2015 SW 16th Ave, Gainesville, FL 32611, USA; Center for Orphan Autoimmune Disorders, University of Florida College of Dentistry, 1600 SW Archer Rd, Gainesville, FL 32610, USA.
authors.forename: Cuong Q
authors.initials: CQ
authors.lastname: Nguyen
keywords: Cytokine; Gene expression; IL-22; Microarray; Sjogren's syndrome