title: Physiological effects and cellular responses of metamorphic larvae and juveniles of sea urchin exposed to ionic and nanoparticulate silver.
abstract: The widespread use of silver nanoparticles (AgNPs) would likely result in their discharge into wastewater and inevitable release in densely populated coastal areas. It is known that AgNPs can cause harmful effects to marine fauna, but how they affect development stages is still an open question. In order to understand in details how polymer-coated AgNPs (PAAm-AgNPs) (from 0.19 to 4.64mM as Ag) can affect critical stages of marine invertebrate development, metamorphic larvae and juveniles of sea urchins were used as biological models. Multidimensional scaling (MDS) approach based on Bray-Curtis similarity matrix with PERMANOVA showed organisms in a multivariate space undergoing through different physiological conditions as a function of time, chemical forms of silver, nominal concentrations, and presence or absence of food. Sublethal effects such as lethargy, oedema and immobility mainly characterized PAAm-AgNPs effects with juveniles and postlarvae, whereas necrosis and death arose in Ag(+) conditions in short-term tests. Chronically exposed metamorphic larvae had their morphogenic processes interrupted by PAAm-AgNPs and a high mortality rate was observed in recovery period. On the contrary, Ag(+) ions caused progressive mortality during exposure, but a quick recovery in uncontaminated seawater was observed. By means of fluorescent markers we showed that nanosilver could be transferred between consecutive stages (swimming larvae and postlarvae) and highlighted how important is food to enhance PAAm-AgNPs uptake. Using TEM we observed that unfed juveniles had nanosilver aggregates mostly restricted to their coelomic sinuses, while metamorphic larvae already had nano-contamination overspread in different tissues and blastocoel. Our main hypothesis for nanotoxicity of PAAM-AgNPs relies on the slow dissolution of nano-core over time, but in this study the effects of particulate silver form itself are also evoked. Main mechanisms governing tissular and cellular responses to nano-intoxication such as inflammatory response and detoxification based on the role of sentinel cells (peritoneal cells and coelomocytes) for general homeostasis are discussed. This paper is first to detail physiological states, main uptake routes and cellular response against polymer-coated AgNPs in developmental stages of marine invertebrate species.
authors.affiliation: Institut de Sciences de la mer (ISMER), Université du Québec à Rimouski. 300, allée des Ursulines, C.P. 3300, succ. A., Rimouski, Québec G5L 3A1, Canada.
authors.forename: Adriano
authors.initials: A
authors.lastname: Magesky
authors.affiliation: Departamento de Biologia Celular, Universidade Federal do Paraná, C.P. 19031, CEP 81531-990 Curitiba, PR, Brazil.
authors.forename: Ciro A Oliveiro
authors.initials: CA
authors.lastname: Ribeiro
authors.affiliation: Institut de Sciences de la mer (ISMER), Université du Québec à Rimouski. 300, allée des Ursulines, C.P. 3300, succ. A., Rimouski, Québec G5L 3A1, Canada. Electronic address: emilien_pelletier@uqar.ca.
authors.forename: Émilien
authors.initials: É
authors.lastname: Pelletier
mesh_terms: D000818:Animals; D007477:Ions / Q000633:toxicity; D007814:Larva / Q000187:drug effects; D053768:Metal Nanoparticles / Q000633:toxicity*; D046529:Microscopy, Electron, Transmission; D012617:Sea Urchins / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D012834:Silver / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D007477:Ions; D014874:Water Pollutants, Chemical; D012834:Silver
keywords: Dissolved silver; Juveniles; Nanoparticles internalization; Organic coated silver nanoparticles toxicity; Sea urchin larvae