title: Analysis of migration rate and chemotaxis of human adipose-derived mesenchymal stem cells in response to LPS and LTA in vitro.
abstract: Mesenchymal stem cells (MSC) are able to stimulate the regeneration of injured tissue. Since bacterial infections are common complications in wound healing, bacterial pathogens and their components come into direct contact with MSC. The interaction with bacterial structures influences the proliferation, differentiation and migratory activity of the MSC, which might be of relevance during regeneration. Studies on MSC migration in response to bacterial components have shown different results depending on the cell type. Here, we analyzed the migration rate and chemotaxis of human adipose-derived MSC (adMSC) in response to the basic cell-wall components lipopolysaccharide (LPS) of Gram-negative bacteria and lipoteichoic acid (LTA) of Gram-positive bacteria in vitro. To this end, we used transwell and scratch assays, as well as a specific chemotaxis assay combined with live-cell imaging. We found no significant influence of LPS or LTA on the migration rate of adMSC in transwell or scratch assays. Furthermore, in the µ-slide chemotaxis assay, the stimulation with LPS did not exert any chemotactic effect on adMSC.
authors.affiliation: Department of Cell Biology, University Medicine Rostock, Schillingallee 69, D-18057 Rostock, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Herzmann
authors.affiliation: Department of Cell Biology, University Medicine Rostock, Schillingallee 69, D-18057 Rostock, Germany.
authors.forename: Achim
authors.initials: A
authors.lastname: Salamon
authors.affiliation: Institute for Medical Microbiology, Virology and Hygiene, University Medicine Rostock, Schillingallee 70, D-18057 Rostock, Germany.
authors.forename: Tomas
authors.initials: T
authors.lastname: Fiedler
authors.affiliation: Department of Cell Biology, University Medicine Rostock, Schillingallee 69, D-18057 Rostock, Germany. Electronic address: kirsten.peters@med.uni-rostock.de.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Peters
mesh_terms: D050153:Abdominal Fat / Q000166:cytology; D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D002633:Chemotaxis / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D008875:Middle Aged; D013682:Teichoic Acids / Q000494:pharmacology*; D055815:Young Adult
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D013682:Teichoic Acids; C009900:lipoteichoic acid
keywords: Adipose-derived mesenchymal stem cells (adMSC); Chemotaxis; Lipopolysaccharide (LPS); Lipoteichoic acid (LTA); Migration