title: cDNA cloning and molecular characterization of a defensin-like antimicrobial peptide from larvae of Protaetia brevitarsis seulensis (Kolbe).
abstract: We identified new defensin-like cDNA (called Psdefensin) by searching data set of high-throughput RNA sequencing (RNA-seq) expression profiling of immunized larva of white-spotted flower chafers, Protaetia brevitarsis seulensis. The length of the analyzed new defensin-like sequences were 240 base pair (bp) and encoded the deduced polypeptide of 79 amino acid residues with signal peptides (amino acids 1-20), pro-peptide region (amino acids 21-36), and mature peptide region (amino acids 37-79). The Psdefensin transcript levels were slightly up-regulated at 4 h post-infection and were highly expressed at 8 h post-infection compared to control larvae injected with sterile water. In addition, the Psdefensin did have antimicrobial activity against both Gram-negative bacteria, E. coli and Gram-positive bacteria, B. subtilis suggesting potentially pharmacologic agent.
authors.affiliation: Department of Applied Biology, Division of Bioresource Science, College of Agriculture and Life Science, Kangwon National University, Kangwon-do, Chuncheon, 200-701, Republic of Korea.
authors.forename: Jiae
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Applied Biology, Division of Bioresource Science, College of Agriculture and Life Science, Kangwon National University, Kangwon-do, Chuncheon, 200-701, Republic of Korea.
authors.forename: Kyeongrin
authors.initials: K
authors.lastname: Bang
authors.affiliation: Department of Applied Biology, Division of Bioresource Science, College of Agriculture and Life Science, Kangwon National University, Kangwon-do, Chuncheon, 200-701, Republic of Korea.
authors.forename: Sejung
authors.initials: S
authors.lastname: Hwang
authors.affiliation: Department of Applied Biology, Division of Bioresource Science, College of Agriculture and Life Science, Kangwon National University, Kangwon-do, Chuncheon, 200-701, Republic of Korea. saeyoullcho@kangwon.ac.kr.|Environmental Friendly Agriculture Center, Kangwon National University, Chuncheon, Republic of Korea. saeyoullcho@kangwon.ac.kr.
authors.forename: Saeyoull
authors.initials: S
authors.lastname: Cho
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D001517:Coleoptera / Q000737:chemistry*; D018076:DNA, Complementary; D023082:Defensins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D019476:Insect Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D007814:Larva / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology
chemical_list: D018076:DNA, Complementary; D023082:Defensins; D019476:Insect Proteins; D011994:Recombinant Proteins
keywords: Antimicrobial peptides; Defensin; Immune; Protaetia brevitarsis seulensis