title: Protective effects of kaempferol against reactive oxygen species-induced hemolysis and its antiproliferative activity on human cancer cells.
abstract: The protective effects of kaempferol against reactive oxygen species (ROS)-induced hemolysis and its antiproliferative activity on human cancer cells were evaluated in this study. Kaempferol exhibited strong cellular antioxidant ability (CAA) with a CAA value of 59.80 ± 0.379 μM of quercetin (QE)/100 μM (EC50 = 7.74 ± 0.049 μM). Pretreatment with kaempferol significantly attenuated the ROS-induced hemolysis of human erythrocyte (87.4% hemolysis suppressed at 100 μg/mL) and reduced the accumulation of toxic lipid peroxidation product malondialdehyde (MDA). The anti-hemolytic activity of kaempferol was mainly through scavenging excessive ROS and preserving the intrinsic antioxidant enzymes (superoxide dismutase, SOD; catalase, CAT; and glutathione peroxidase, GPx) activities in normal levels. Additionally, kaempferol showed significant antiproliferative activity on a panel of human cancer cell lines including human breast carcinoma (MCF-7) cells, human stomach carcinoma (SGC-7901) cells, human cervical carcinoma (Hela) cells and human lung carcinoma (A549) cells. Kaemperol induced apoptosis of MCF-7 cells accompanied with nuclear condensation and mitochondria dysfunction.
authors.affiliation: Department of Food Science and Engineering, Jinan University, 601 West, Huangpu Road, Guangzhou, 510632, China; College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, 510640, China.
authors.forename: Wenzhen
authors.initials: W
authors.lastname: Liao
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, 510640, China.
authors.forename: Luying
authors.initials: L
authors.lastname: Chen
authors.affiliation: Research School of Chemistry, The Australian National University, Canberra ACT 2601, Australia.
authors.forename: Xiang
authors.initials: X
authors.lastname: Ma
authors.affiliation: Department of Food Science and Engineering, Jinan University, 601 West, Huangpu Road, Guangzhou, 510632, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Jiao
authors.affiliation: Department of Food Science and Engineering, Jinan University, 601 West, Huangpu Road, Guangzhou, 510632, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Food Science and Engineering, Jinan University, 601 West, Huangpu Road, Guangzhou, 510632, China. Electronic address: twyong@jnu.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D006367:HeLa Cells; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D044949:Kaempferols / Q000737:chemistry / Q000494:pharmacology*; D061986:MCF-7 Cells; D015394:Molecular Structure; D009369:Neoplasms / Q000473:pathology*; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D000975:Antioxidants; D044949:Kaempferols; D020011:Protective Agents; D017382:Reactive Oxygen Species; C006552:kaempferol
keywords: Anti-hemolytic activity; Antiproliferative activity; Apoptosis; Cellular antioxidant ability; Kaempferol; Reactive oxygen species