title: [(η(6)-p-cymene)Ru(H2O)3](2+) binding capability of aminohydroxamates - A solution and solid state study.
abstract: Complex forming capabilities of [(η(6)-p-cymene)Ru(H2O)3](2+) with aminohydroxamates (2-amino-N-hydroxyacetamide (α-alahaH), 3-amino-N-hydroxypropanamide (β-alahaH) and 4-amino-N-hydroxybutanamide (γ-abhaH)) having the primary amino group in different chelatable position to the hydroxamic function were studied by pH-potentiometry, NMR and MS methods. Formation of stable [O,O] and mixed [O,O][N,N] chelated mono- and dinuclear species is detected in partially slow with α-alahaH and β-alahaH or in fast processes with γ-abhaH and the formation constants of the complexes present in aqueous solution are reported. Synthesis, spectral (NMR, IR) and ESI mass spectrometric characterization of novel dinuclear α-alaninehydroximato complexes containing the half-sandwich type Ru(II) core is described. The crystal and molecular structure of [{(η(6)-p-cymene)Ru}2(μ(2)-α-alahaH-1)(H2O)Br]Br∙H2O (1) and [{(η(6)-p-cymene)Ru}2(μ(2)-α-alahaH-1)(H2O)Cl]BF4∙H2O (2) was determined by single crystal X-ray diffraction method. In the complexes one half-sandwich core is coordinated by a hydroxamate [O,O] chelate while the other one by [Namino,Nhydroxamate] fashion of the bridging ligand. In both cases the remaining coordination sites of one of the Ru cores are taken by a halide ion whiles the other one by a water molecule. Reaction of 2 with 9-methylguanine indicates the N7 coordination of this simple DNA model. Complexes 1 and 2 were tested for their in vitro cytotoxicity using human-derived cancer cell lines (A2780, MCF-7, SKOV-3, HCT-116, HeLa) and showed no anti-proliferative activity in the micromolar concentration range.
authors.affiliation: Department of Inorganic and Analytical Chemistry, University of Debrecen, H-4010 Debrecen, P.O. Box 21, Hungary.
authors.forename: Péter László
authors.initials: PL
authors.lastname: Parajdi-Losonczi
authors.affiliation: Department of Physical Chemistry, University of Debrecen, H-4010 Debrecen, P.O.Box 7, Hungary.
authors.forename: Attila Csaba
authors.initials: AC
authors.lastname: Bényei
authors.affiliation: HAS Wigner Research Centre for Physics, 1121 Budapest, Konkoly Thege Miklós u. 29-33, Hungary.
authors.forename: Éva
authors.initials: É
authors.lastname: Kováts
authors.affiliation: Department of Inorganic and Analytical Chemistry, University of Debrecen, H-4010 Debrecen, P.O. Box 21, Hungary.
authors.forename: István
authors.initials: I
authors.lastname: Timári
authors.affiliation: Department of Biophysics, Faculty of Science, Palacky University, 17. Listopadu 12, 77146 Olomouc, Czech Republic.
authors.forename: Tereza Radosova
authors.initials: TR
authors.lastname: Muchova
authors.affiliation: Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i. Kralovopolska 135, 61565 Brno, Czech Republic.
authors.forename: Vojtech
authors.initials: V
authors.lastname: Novohradsky
authors.affiliation: Department of Biophysics, Faculty of Science, Palacky University, 17. Listopadu 12, 77146 Olomouc, Czech Republic; Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i. Kralovopolska 135, 61565 Brno, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Kasparkova
authors.affiliation: Department of Inorganic and Analytical Chemistry, University of Debrecen, H-4010 Debrecen, P.O. Box 21, Hungary. Electronic address: buglyo@science.unideb.hu.
authors.forename: Péter
authors.initials: P
authors.lastname: Buglyó
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D002413:Cations, Divalent; D045744:Cell Line, Tumor; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000494:pharmacology; D018360:Crystallography, X-Ray; D000081007:Cymenes; D006147:Guanine / Q000031:analogs & derivatives / Q000737:chemistry; D045325:HCT116 Cells; D006367:HeLa Cells; D006801:Humans; D006877:Hydroxamic Acids / Q000737:chemistry*; D061986:MCF-7 Cells; D008958:Models, Molecular; D039821:Monoterpenes / Q000737:chemistry*; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000494:pharmacology; D012428:Ruthenium / Q000737:chemistry*; D005680:gamma-Aminobutyric Acid / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D002413:Cations, Divalent; D056831:Coordination Complexes; D000081007:Cymenes; D006877:Hydroxamic Acids; D039821:Monoterpenes; D009942:Organometallic Compounds; C007210:4-cymene; C116701:9-methylguanine; C006358:acetohydroxamic acid; C023814:4-amino-3-hydroxybutanamide; D005680:gamma-Aminobutyric Acid; D006147:Guanine; D012428:Ruthenium
keywords: Aminohydroxamates; Anticancer; Ruthenium complexes; Speciation; X-ray structure