title: Vascular responses to compound 48/80 in rat mesenteric vascular beds.
abstract: A further investigation was performed on the vascular effect of endogenous histamine using the histamine releaser, compound 48/80, in rat mesenteric vascular beds with active tone. In preparations with intact endothelium, low concentrations of compound 48/80 (1.53 × 10(-5) - 3 × 1.53 × 10(-5) mg/mL) perfusion for 1 min only induced a small vasodilation. High concentrations of compound 48/80 (1.53 × 10(-4) - 3 × 1.53 × 10(-2) mg/mL) induced a biphasic vascular responses, an initial vasoconstriction followed a subsequent long-lasting vasodilation. The vasodilation induced by low concentrations of compound 48/80 and the vasoconstriction induced by high concentration of compound 48/80 was inhibited by olopatadine. However, cimetidine did not affect the responses induced by compound 48/80. Endothelium removal enlarged the compound 48/80-induced phase-2 vasoconstriction, while it attenuated the phase-3 vasodilation. Additionally, indomethacin and seratrodast significantly inhibited vasoconstriction but it did not affect the long-lasting vasodilation induced by high concentrations of compound 48/80. Ruthenium red inhibited the vasodilation induced by low concentrations and high concentrations of compound 48/80. These results suggest that the vasoconstriction induce by high concentrations of compound 48/80 is mediated by endogenous histamine released from mast cells. It is also suggested that thromboxane A2 released from mast cells is related to the vasoconstriction.
authors.affiliation: a Department of Pharmacy, Affiliated Hospital of Yanbian University, Yanji 133000, China.
authors.forename: Honghua
authors.initials: H
authors.lastname: Jin
authors.affiliation: b Department of Pharmacology, College of Pharmacy, Dalian Medical University, Dalian 116044, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.affiliation: c Department of Clinical Pharmaceutical Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8530, Japan.|d Department of Clinical Pharmacy, College of Pharmaceutical Sciences, Matsuyama University, Matsuyama, Ehime 790-8578, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Takatori
authors.affiliation: c Department of Clinical Pharmaceutical Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8530, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Koyama
authors.affiliation: c Department of Clinical Pharmaceutical Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8530, Japan.
authors.forename: Xin
authors.initials: X
authors.lastname: Jin
authors.affiliation: c Department of Clinical Pharmaceutical Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8530, Japan.
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Zamami
authors.affiliation: c Department of Clinical Pharmaceutical Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8530, Japan.|d Department of Clinical Pharmacy, College of Pharmaceutical Sciences, Matsuyama University, Matsuyama, Ehime 790-8578, Japan.
authors.forename: Hiromu
authors.initials: H
authors.lastname: Kawasaki
authors.affiliation: b Department of Pharmacology, College of Pharmacy, Dalian Medical University, Dalian 116044, China.
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Sun
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology; D008297:Male; D008638:Mesenteric Arteries / Q000187:drug effects* / Q000502:physiology; D009924:Organ Culture Techniques; D051381:Rats; D017208:Rats, Wistar; D014661:Vasoconstriction / Q000187:drug effects* / Q000502:physiology; D014664:Vasodilation / Q000187:drug effects* / Q000502:physiology; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology*
chemical_list: D003189:p-Methoxy-N-methylphenethylamine
keywords: artère de résistance mésentérique du rat; composé 48/80; compound 48/80; endogenous histamine; histamine endogène; master cell; mastocytes; perfusion; rat mesenteric resistance artery; réponse vasculaire; vascular response