title: Arcobacter butzleri survives within trophozoite of Acanthamoeba castellanii.
abstract: The survival of three Arcobacter butzleri strains inside Acanthamoeba castellanii was assessed using axenic cultures of A. castellanii that were inoculated with the tested strains and incubated at 26°C under aerobic conditions for 240h. The behavior of bacteria in contact with amoebae was monitored using phase contrast microscopy. The bacterial survival rate within amoebae was assessed through counting colony forming units, using the gentamicin protection assay. All A. butzleri strains were able to survive during 240h within the amoebae, thus suggesting that (i) A. butzleri resists the amoebic digestion processes at least for the analyzed time; (ii) that A. castellanii could serve as an environmental reservoir for this bacterium, probably acting as a transmission vehicle for A. butzleri.
authors.affiliation: Instituto de Microbiología Clínica, Universidad Austral de Chile, PO Box 567, Valdivia, Chile.
authors.forename: María P
authors.initials: MP
authors.lastname: Villanueva
authors.affiliation: Facultad de Ciencias de la Salud, Universidad Católica de Temuco, PO Box 15-D, Temuco, Chile.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Medina
authors.affiliation: Instituto de Microbiología Clínica, Universidad Austral de Chile, PO Box 567, Valdivia, Chile. Electronic address: hfernand@uch.cl.
authors.forename: Heriberto
authors.initials: H
authors.lastname: Fernández
mesh_terms: D048248:Acanthamoeba castellanii / Q000254:growth & development / Q000382:microbiology* / Q000648:ultrastructure; D000332:Aerobiosis; D020640:Arcobacter / Q000502:physiology*; D061245:Axenic Culture; D004197:Disease Reservoirs; D008858:Microscopy, Phase-Contrast; D014617:Vacuoles / Q000382:microbiology / Q000648:ultrastructure; D014871:Water Microbiology
keywords: Acanthamoeba castellanii; Arcobacter butzleri; Environmental reservoir; Reservorio ambiental; Sobrevida; Survival