title: A novel dengue virus serotype 1 vaccine candidate based on Japanese encephalitis virus vaccine strain SA14-14-2 as the backbone.
abstract: To develop a potential dengue vaccine candidate, a full-length cDNA clone of a novel chimeric virus was constructed using recombinant DNA technology, with Japanese encephalitis virus (JEV) vaccine strain SA14-14-2 as the backbone, with its premembrane (prM) and envelope (E) genes substituted by their counterparts from dengue virus type 1 (DENV1). The chimeric virus (JEV/DENV1) was successfully recovered from primary hamster kidney (PHK) cells by transfection with the in vitro transcription products of JEV/DENV1 cDNA and was identified by complete genome sequencing and immunofluorescent staining. No neuroinvasiveness of this chimeric virus was observed in mice inoculated by the subcutaneous route (s.c.) or by the intraperitoneal route (i.p.), while some neurovirulence was displayed in mice that were inoculated directly by the intracerebral route (i.c.). The chimeric virus was able to stimulate high-titer production of antibodies against DENV1 and provided protection against lethal challenge with neuroadapted dengue virus in mice. These results suggest that the chimeric virus is a promising dengue vaccine candidate.
authors.affiliation: Department of Viral Vaccine, Chengdu Institute of Biological Products Co., Ltd, China National Biotech Group, Chengdu, 610023, People's Republic of China.
authors.forename: Huiqiang
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Viral Vaccine, Chengdu Institute of Biological Products Co., Ltd, China National Biotech Group, Chengdu, 610023, People's Republic of China.
authors.forename: Zhushi
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Viral Vaccine, Chengdu Institute of Biological Products Co., Ltd, China National Biotech Group, Chengdu, 610023, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Lin
authors.affiliation: Department of Viral Vaccine, Chengdu Institute of Biological Products Co., Ltd, China National Biotech Group, Chengdu, 610023, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Viral Vaccine, Chengdu Institute of Biological Products Co., Ltd, China National Biotech Group, Chengdu, 610023, People's Republic of China.|Department of Microbiology and Immunology, North Sichuan Medical College, Nanchong, 637007, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Viral Vaccine, Chengdu Institute of Biological Products Co., Ltd, China National Biotech Group, Chengdu, 610023, People's Republic of China.
authors.forename: Lina
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Viral Vaccine, Chengdu Institute of Biological Products Co., Ltd, China National Biotech Group, Chengdu, 610023, People's Republic of China.
authors.forename: Xianwu
authors.initials: X
authors.lastname: Zeng
authors.affiliation: China National Biotech Group Co., Ltd, Beijing, 100029, People's Republic of China.
authors.forename: Yonglin
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Arbovirus Vaccine, National Institutes for Food and Drug Control, No. 2, Tiantan Xili, Chongwen District, Beijing, 100050, People's Republic of China.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Arbovirus Vaccine, National Institutes for Food and Drug Control, No. 2, Tiantan Xili, Chongwen District, Beijing, 100050, People's Republic of China. liyuhua@hotmail.com.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000096:biosynthesis; D000914:Antibodies, Viral / Q000096:biosynthesis; D002460:Cell Line; D006224:Cricetinae; D003715:Dengue / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D053059:Dengue Vaccines / Q000235:genetics* / Q000276:immunology*; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics / Q000276:immunology*; D004664:Encephalitis Virus, Japanese / Q000235:genetics* / Q000276:immunology*; D005260:Female; D016679:Genome, Viral; D006801:Humans; D022321:Japanese Encephalitis Vaccines / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D065288:Serogroup; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology; D010948:Viral Plaque Assay
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D053059:Dengue Vaccines; D022321:Japanese Encephalitis Vaccines; D014614:Vaccines, Synthetic