title: Detectors for evaluating the cellular landscape of sphingomyelin- and cholesterol-rich membrane domains.
abstract: Although sphingomyelin and cholesterol are major lipids of mammalian cells, the detailed distribution of these lipids in cellular membranes remains still obscure. However, the recent development of protein probes that specifically bind sphingomyelin and/or cholesterol provides new information about the landscape of the lipid domains that are enriched with sphingomyelin or cholesterol or both. Here, we critically summarize the tools to study distribution and dynamics of sphingomyelin and cholesterol. This article is part of a Special Issue entitled: The cellular lipid landscape edited by Tim P. Levine and Anant K. Menon.
authors.affiliation: Lipid Biology Laboratory, RIKEN, Wako, Saitama 351-0198, Japan.
authors.forename: Takuma
authors.initials: T
authors.lastname: Kishimoto
authors.affiliation: Lipid Biology Laboratory, RIKEN, Wako, Saitama 351-0198, Japan.
authors.forename: Reiko
authors.initials: R
authors.lastname: Ishitsuka
authors.affiliation: Lipid Biology Laboratory, RIKEN, Wako, Saitama 351-0198, Japan; INSERM U1060, Université Lyon 1, Villeurbanne 69621, France. Electronic address: kobayasi@riken.jp.
authors.forename: Toshihide
authors.initials: T
authors.lastname: Kobayashi
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry; D006801:Humans; D008563:Membrane Lipids / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D021962:Membrane Microdomains / Q000737:chemistry* / Q000378:metabolism*; D015336:Molecular Probe Techniques; D015335:Molecular Probes / Q000032:analysis / Q000737:chemistry; D008970:Molecular Weight; D013109:Sphingomyelins / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D008563:Membrane Lipids; D015335:Molecular Probes; D013109:Sphingomyelins; D002784:Cholesterol
keywords: Aegerolysin; Equinatoxin II; Lipid domains; Lipid imaging; Lysenin; Perfringolysin O