title: Systematic Analysis of Absorbed Anti-Inflammatory Constituents and Metabolites of Sarcandra glabra in Rat Plasma Using Ultra-High-Pressure Liquid Chromatography Coupled with Linear Trap Quadrupole Orbitrap Mass Spectrometry.
abstract: Ultra-high-pressure liquid chromatography (UHPLC) was coupled with linear ion trap quadrupole Orbitrap mass spectrometry (LTQ-Orbitrap) and was used for the first time to systematically analyze the absorbed components and metabolites in rat plasma after oral administration of the water extract of Sarcandra glabra. This extract is a well-known Chinese herbal medicine for the treatment of inflammation and immunity related diseases. The anti-inflammatory activities of the absorbed components were evaluated by measuring nitric oxide (NO) production and proinflammatory genes expression in lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophages. As a result, 54 components in Sarcandra glabra were detected in dosed rat plasma, and 36 of them were positively identified. Moreover, 23 metabolites were characterized and their originations were traced. Furthermore, 20 of the 24 studied components showed anti-inflammatory activities. These results provide evidence that this method efficiency detected constituents in plasma based on the anti-inflammatory mechanism of multiple components and would be a useful technique for screening multiple targets for natural medicine research.
authors.affiliation: Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Li
authors.affiliation: Zhongshan City People's Hospital, Zhongshan, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhao
authors.affiliation: School of Chinese Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou, China.
authors.forename: Jianxing
authors.initials: J
authors.lastname: Liu
authors.affiliation: School of Chinese Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou, China.
authors.forename: Geng
authors.initials: G
authors.lastname: Li
authors.affiliation: Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, China.|The postdoctoral research station, Guangzhou University of Chinese Medicine, Guangzhou, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Zeng
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D019684:Magnoliopsida / Q000737:chemistry*; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts