title: Delivery of mesenchymal stem cells in biomimetic engineered scaffolds promotes healing of diabetic ulcers.
abstract: OBJECTIVE
  We hypothesized that delivery of mesenchymal stem cells (MSCs) in a biomimetic collagen scaffold improves wound healing in a diabetic mouse model.
  
  
  METHODS
  Rolled collagen scaffolds containing MSCs were implanted or applied topically to diabetic C57BL/6 mice with excisional wounds.
  
  
  RESULTS
  Rolled scaffolds were hypoxic, inducing MSC synthesis and secretion of VEGF. Diabetic mice with wounds treated with rolled scaffolds containing MSCs showed increased healing compared with controls. Histologic examination showed increased cellular proliferation, increased VEGF expression and capillary density, and increased numbers of macrophages, fibroblasts and smooth muscle cells. Addition of laminin to the collagen scaffold enhanced these effects.
  
  
  CONCLUSIONS
  Activated MSCs delivered in a biomimetic-collagen scaffold enhanced wound healing in a translationally relevant diabetic mouse model.
authors.affiliation: Vascular Biology & Therapeutics Program & the Department of Surgery, Yale University School of Medicine, New Haven, CT, USA.
authors.forename: Roland
authors.initials: R
authors.lastname: Assi
authors.affiliation: Vascular Biology & Therapeutics Program & the Department of Surgery, Yale University School of Medicine, New Haven, CT, USA.
authors.forename: Trenton R
authors.initials: TR
authors.lastname: Foster
authors.affiliation: Vascular Biology & Therapeutics Program & the Department of Surgery, Yale University School of Medicine, New Haven, CT, USA.|Department of Vascular Surgery, The Second Xiangya Hospital of Central South University, Changsha, China.
authors.forename: Hao
authors.initials: H
authors.lastname: He
authors.affiliation: UCL Institute of Orthopaedics & Musculoskeletal Sciences, UCL Division of Surgery & Interventional Sciences, University College London, London, UK.
authors.forename: Katerina
authors.initials: K
authors.lastname: Stamati
authors.affiliation: Vascular Biology & Therapeutics Program & the Department of Surgery, Yale University School of Medicine, New Haven, CT, USA.
authors.forename: Hualong
authors.initials: H
authors.lastname: Bai
authors.affiliation: Departments of Diagnostic Radiology & Biomedical Engineering, Yale University, New Haven, CT, USA.
authors.forename: Yuegao
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Departments of Diagnostic Radiology & Biomedical Engineering, Yale University, New Haven, CT, USA.
authors.forename: Fahmeed
authors.initials: F
authors.lastname: Hyder
authors.affiliation: Departments of Diagnostic Radiology & Biomedical Engineering, Yale University, New Haven, CT, USA.
authors.forename: Douglas
authors.initials: D
authors.lastname: Rothman
authors.affiliation: Department of Vascular Surgery, The Second Xiangya Hospital of Central South University, Changsha, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Shu
authors.affiliation: UCL Institute of Orthopaedics & Musculoskeletal Sciences, UCL Division of Surgery & Interventional Sciences, University College London, London, UK.
authors.forename: Shervanthi
authors.initials: S
authors.lastname: Homer-Vanniasinkam
authors.affiliation: UCL Institute of Orthopaedics & Musculoskeletal Sciences, UCL Division of Surgery & Interventional Sciences, University College London, London, UK.
authors.forename: Umber
authors.initials: U
authors.lastname: Cheema
authors.affiliation: Vascular Biology & Therapeutics Program & the Department of Surgery, Yale University School of Medicine, New Haven, CT, USA.|Department of Surgery, VA Connecticut Healthcare Systems, West Haven, CT, USA.
authors.forename: Alan
authors.initials: A
authors.lastname: Dardik
mesh_terms: D064591:Allografts; D000818:Animals; D040761:Biomimetic Materials; D018914:Cells, Immobilized / Q000378:metabolism / Q000637:transplantation; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000628:therapy*; D003925:Diabetic Angiopathies / Q000378:metabolism / Q000628:therapy*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice; D018919:Neovascularization, Physiologic; D054457:Tissue Scaffolds; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis
chemical_list: D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse
keywords: VEGF; angiogenesis; collagen I; diabetic ulcer; extracellular matrix proteins; laminin; mesenchymal stem cells; tissue engineering; tissue scaffolds