title: Repulsive guidance molecule a blockade exerts the immunoregulatory function in DCs stimulated with ABP and LPS.
abstract: Repulsive guidance molecule a (RGMa) is an axonal guidance molecule that has recently found to exert function in immune system. This study evaluated the function of RGMa in modulation of dendritic cells (DCs) function stimulated with Achyranthes bidentata polysaccharide (ABP) and lipopolysaccharide (LPS) using a RGMa-neutralizing antibody. Compared with the Control-IgG/ABP and Control-IgG/LPS groups, DCs in the Anti-RGMa/ABP and Anti-RGMa/LPS groups 1) showed small, round cells with a few cell processes and organelles, and many pinocytotic vesicles; 2) had decreased MHC II, CD86, CD80, and CD40 expression; 3) displayed the decreased IL-12p70, IL-1β and TNF-α levels and increased IL-10 secretion; 4) had a high percentage of FITC-dextran uptake; and 5) displayed a reduced ability to drive T cell proliferation and reinforced T cell polarization toward a Th2 cytokine pattern. We conclude that DCs treated with RGMa-neutralizing antibodies present with tolerogenic and immunoregulatory characteristics, which provides new insights into further understanding of the function of RGMa.
authors.affiliation: a Department of Neurology, Shengjing Hospital , Affiliated Hospital of China Medical University , Shenyang , PR China.
authors.forename: Xuxu
authors.initials: X
authors.lastname: Xu
authors.affiliation: a Department of Neurology, Shengjing Hospital , Affiliated Hospital of China Medical University , Shenyang , PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: a Department of Neurology, Shengjing Hospital , Affiliated Hospital of China Medical University , Shenyang , PR China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Zhai
authors.affiliation: a Department of Neurology, Shengjing Hospital , Affiliated Hospital of China Medical University , Shenyang , PR China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Zhang
authors.affiliation: b Department of Immunology, School of Basic Medical Science , China Medical University , Shenyang , PR China.
authors.forename: Fengping
authors.initials: F
authors.lastname: Shan
authors.affiliation: a Department of Neurology, Shengjing Hospital , Affiliated Hospital of China Medical University , Shenyang , PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Feng
mesh_terms: D029044:Achyranthes / Q000276:immunology; D000818:Animals; D001419:Bacteria / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology*; D058851:GPI-Linked Proteins / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011134:Polysaccharides / Q000276:immunology*
chemical_list: D058851:GPI-Linked Proteins; D009419:Nerve Tissue Proteins; D011134:Polysaccharides; C483496:Rgma protein, mouse
keywords: ABP; LPS; RGMa; dendritic cells; immune regulation; tolerance