title: Identification, expression, and innate immune responses of two insulin-like peptide genes in the razor clam Sinonovacula constricta.
abstract: Insulin-like peptide (ILP) has emerged as a cell regulatory factor with multiple functions in vertebrates and invertebrates. In the present study, we identified and characterized two ILP genes, ILP1 and ILP2, in the razor clam Sinonovacula constricta. Both ILPs have a signal peptide and a mature domain consisting of six strictly conserved cysteines. The tertiary structure is divided into three main α-helices with a C-domain loop that separates helix 1 from helix 2. Both of ILPs were found to be regulated according to tissue type and developmental stage. After challenge with Vibrio anguillarum, Vibrio parahaemolyticus and Micrococcus lysodeikticus, the expression of two ILP genes was significantly up-regulated in the liver, hemocytes and mantle tissues, suggesting that the ILPs may play roles in the innate immunity in the razor clam Sinonovacula constricta.
authors.affiliation: Shanghai Engineering Research Center of Aquaculture and College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Donghong
authors.initials: D
authors.lastname: Niu
authors.affiliation: Shanghai Engineering Research Center of Aquaculture and College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Shanghai Engineering Research Center of Aquaculture and College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China; Department of Fisheries and Allied Aquacultures, Auburn University, Auburn, AL 36849, USA.
authors.forename: Honggang
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Shanghai Engineering Research Center of Aquaculture and College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Ze
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Shanghai Engineering Research Center of Aquaculture and College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Shumei
authors.initials: S
authors.lastname: Xie
authors.affiliation: Shanghai Engineering Research Center of Aquaculture and College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China. Electronic address: jlli2009@126.com.
authors.forename: Jiale
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D001424:Bacterial Infections / Q000235:genetics* / Q000276:immunology / Q000662:veterinary; D049872:Bivalvia / Q000235:genetics* / Q000276:immunology*; D006434:Hemocytes / Q000378:metabolism; D007113:Immunity, Innate; D008099:Liver / Q000378:metabolism; D008837:Micrococcus; D036361:Peptide Hormones / Q000235:genetics* / Q000276:immunology*; D010802:Phylogeny; D014733:Vibrio
chemical_list: D036361:Peptide Hormones
keywords: ILP; Innate immunity; Insulin-like peptide; Sinonovacula constricta