title: Determination and pharmacokinetic study of four lignans in rat plasma after oral administration of an extract of Valeriana amurensis by ultra-high performance liquid chromatography with tandem mass spectrometry.
abstract: A selective and sensitive ultra-high performance liquid chromatography with tandem mass spectrometry method was developed and validated for the determination and pharmacokinetic study of (+)-8-hydroxypinoresinol-4'-O-β -D-glucopyranoside, prinsepiol-4-O-β-D-glucopyranoside, (+)-pinoresinol-4,4'-di-O-β-D-glucopyranoside, and (-)-massoniresinol 3α-O-β-D-glucopyranoside in rat plasma after the oral administration of a Valeriana amurensis extract. The analytes and ethyl 4-hydroxybenzoate (internal standard) were separated on a Waters ACQUITY UPLC HSS T3 chromatographic column. The detection was performed on a triple quadrupole tandem mass spectrometer in multiple reaction monitoring mode using an electrospray ionization source operating in negative ionization mode. The linear ranges (ng/mL) of the standard curves were 0.39-154.00, 0.62-244.70, 0.50-198.60, and 0.34-134.50 for (+)-8-hydroxypinoresinol-4'-O-β-D-glucopyranoside, prinsepiol-4-O-β-D-glucopyranoside, (+)-pinoresinol-4,4'-di-O-β-D-glucopyranoside, and (-)-massoniresinol 3α-O-β-D-glucopyranoside, respectively. The inter- and intra-day precisions were less than 11.0%, the accuracies were between -5.9 and 7.7%, and the extraction recoveries of the four analytes were > 81.2% from rat plasma. The method was successfully applied to a pharmacokinetic study of the four analytes after oral administration of a Valeriana amurensis extract to rats. The developed method has the potential for pharmacokinetic analysis and to provide additional information in the clinical application of Valeriana amurensis.
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, 24 Heping Road, Xiangfang District, Harbin, 150040, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Xue
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, 24 Heping Road, Xiangfang District, Harbin, 150040, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Mi
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, 24 Heping Road, Xiangfang District, Harbin, 150040, China.
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, 24 Heping Road, Xiangfang District, Harbin, 150040, China.
authors.forename: Yichun
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, 24 Heping Road, Xiangfang District, Harbin, 150040, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, 24 Heping Road, Xiangfang District, Harbin, 150040, China.
authors.forename: Changfu
authors.initials: C
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, 24 Heping Road, Xiangfang District, Harbin, 150040, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, 24 Heping Road, Xiangfang District, Harbin, 150040, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, 24 Heping Road, Xiangfang District, Harbin, 150040, China.
authors.forename: Haixue
authors.initials: H
authors.lastname: Kuang
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, 24 Heping Road, Xiangfang District, Harbin, 150040, China.
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D017705:Lignans / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D008297:Male; D015394:Molecular Structure; D010936:Plant Extracts / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry; D014632:Valerian / Q000737:chemistry*
chemical_list: D017705:Lignans; D010936:Plant Extracts
keywords: Lignan; Pharmacokinetics; Tandem mass spectrometry; Valeriana amurensis