title: Generation of integration-free induced pluripotent stem cells from a patient with Familial Mediterranean Fever (FMF).
abstract: Fibroblasts from a Familial Mediterranean Fever (FMF) patient were reprogrammed with episomal vectors by using the Neon Transfection System for the generation of integration-free induced pluripotent stem cells (iPSCs). The resulting iPSC line was characterized to determine the expression of pluripotency markers, proper differentiation into three germ layers, the presence of normal chromosomal structures as well as the lack of genomic integration. A homozygous missense mutation in the MEFV gene (p.Met694Val), which lead to typical FMF phenotype, was shown to be present in the generated iPSC line.
authors.affiliation: School of Medicine, Koc University, Istanbul 34450, Turkey.
authors.forename: Kerem
authors.initials: K
authors.lastname: Fidan
authors.affiliation: School of Medicine, Koc University, Istanbul 34450, Turkey.
authors.forename: Gülnihal
authors.initials: G
authors.lastname: Kavaklıoğlu
authors.affiliation: School of Medicine, Koc University, Istanbul 34450, Turkey.
authors.forename: Ayyub
authors.initials: A
authors.lastname: Ebrahimi
authors.affiliation: School of Medicine, Koc University, Istanbul 34450, Turkey.
authors.forename: Can
authors.initials: C
authors.lastname: Özlü
authors.affiliation: School of Medicine, Koc University, Istanbul 34450, Turkey.
authors.forename: Nur Zeynep
authors.initials: NZ
authors.lastname: Ay
authors.affiliation: School of Medicine, Koc University, Istanbul 34450, Turkey.
authors.forename: Arzu
authors.initials: A
authors.lastname: Ruacan
authors.affiliation: Faculty of Medicine, Istanbul University, Istanbul 34093, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Gül
authors.affiliation: School of Medicine, Koc University, Istanbul 34450, Turkey.
authors.forename: Tamer T
authors.initials: TT
authors.lastname: Önder
mesh_terms: D002454:Cell Differentiation; D010505:Familial Mediterranean Fever / Q000276:immunology* / Q000473:pathology; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*