title: miR-152 induces human dental pulp stem cell senescence by inhibiting SIRT7 expression.
abstract: Human dental pulp stem cells (HDPSCs) have potential applications in regenerative medicine. The molecular mechanisms underlying HDPSC senescence are not completely understood. Here, we investigated the significance of miR-152 in HDPSC senescence. We show that miR-152 is upregulated during HDPSC senescence and its overexpression in early passaged HDPSCs induced senescence. Sirtuin 7 (SIRT7) was identified as a target of miR-152. SIRT7 was downregulated in senescent HDPSCs, whereas miR-152 inhibition upregulated SIRT7 and suppressed the senescent phenotype and SIRT7 overexpression rescued miR-152-induced senescence. Our results demonstrate that the miR-152/SIRT7 axis plays a key role in the regulation of HDPSC senescence and provide a candidate target to improve the functional and therapeutic potential of HDPSCs.
authors.affiliation: Department of Endodontics, Ninth People's Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai Key Laboratory of Stomatology, China.
authors.forename: Shensheng
authors.initials: S
authors.lastname: Gu
authors.affiliation: Department of Endodontics, Ninth People's Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai Key Laboratory of Stomatology, China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Ran
authors.affiliation: Department of Endodontics, Ninth People's Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai Key Laboratory of Stomatology, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Endodontics, Ninth People's Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai Key Laboratory of Stomatology, China.
authors.forename: Jingping
authors.initials: J
authors.lastname: Liang
mesh_terms: D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D049109:Cell Proliferation; D016922:Cellular Senescence / Q000235:genetics*; D003782:Dental Pulp / Q000166:cytology*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D037761:Sirtuins / Q000235:genetics* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*; D014158:Transcription, Genetic; D015854:Up-Regulation / Q000235:genetics; D055815:Young Adult
chemical_list: C550856:MIRN152 microRNA, human; D035683:MicroRNAs; C510661:SIRT7 protein, human; D037761:Sirtuins
keywords: SIRT7; dental pulp stem cells; miR-152; senescence