title: Bioaccumulation characterization of uranium by a novel Streptomyces sporoverrucosus dwc-3.
abstract: The biosorption mechanisms of uranium on an aerobic bacterial strain Streptomyces sporoverrucosus dwc-3, isolated from a potential disposal site for (ultra-)low uraniferous radioactive waste in Southwest China, were evaluated by using transmission electron microscopy (TEM), energy dispersive X-ray (EDX) analysis, Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), proton induced X-ray emission (PIXE) and enhanced proton backscattering spectrometry (EPBS). Approximately 60% of total uranium at an initial concentration of 10mg/L uranium nitrate solution could be absorbed on 100mg S. sporoverrucosus dwc-3 with an adsorption capacity of more than 3.0mg/g (wet weight) after 12hr at room temperature at pH3.0. The dynamic biosorption process of S. sporoverrucosus dwc-3 for uranyl ions was well described by a pseudo second-order model. S. sporoverrucosus dwc-3 could accumulate uranium on cell walls and within the cell, as revealed by SEM and TEM analysis as well as EDX spectra. XPS and FT-IR analysis further suggested that the absorbed uranium was bound to amino, phosphate and carboxyl groups of the cells. Additionally, PIXE and EPBS results confirmed that ion exchange also contributed to the adsorption process of uranium.
authors.affiliation: Key Laboratory of Radiation Physics and Technology, Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064, China. Electronic address: lixiaolongscu720@126.com.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Radiation Physics and Technology, Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064, China; Key Laboratory of Biological Resource and Ecological Environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China.
authors.forename: Congcong
authors.initials: C
authors.lastname: Ding
authors.affiliation: Key Laboratory of Radiation Physics and Technology, Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064, China. Electronic address: liaojiali@scu.edu.cn.
authors.forename: Jiali
authors.initials: J
authors.lastname: Liao
authors.affiliation: Institute of Nuclear Physics and Chemistry, CAEP, Mianyang 621900, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Du
authors.affiliation: Key Laboratory of Biological Resource and Ecological Environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Key Laboratory of Radiation Physics and Technology, Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064, China.
authors.forename: Jijun
authors.initials: J
authors.lastname: Yang
authors.affiliation: Key Laboratory of Radiation Physics and Technology, Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064, China.
authors.forename: Yuanyou
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Institute of Nuclear Physics and Chemistry, CAEP, Mianyang 621900, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Radiation Physics and Technology, Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Tang
authors.affiliation: Key Laboratory of Radiation Physics and Technology, Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064, China. Electronic address: nliu720@scu.edu.cn.
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
mesh_terms: D001673:Biodegradation, Environmental; D002681:China; D004784:Environmental Monitoring; D012989:Soil Pollutants / Q000378:metabolism*; D013302:Streptomyces / Q000302:isolation & purification / Q000378:metabolism*; D014501:Uranium / Q000378:metabolism*
chemical_list: D012989:Soil Pollutants; D014501:Uranium
keywords: Bioaccumulation; Bioremediation; Nanoparticle; Streptomyces sporoverrucosus dwc-3; Uranium