title: Significantly reduced adsorption and activation of blood components in a membrane oxygenator system coated with crosslinkable zwitterionic copolymer.
abstract: A crosslinkable zwitterionic copolymer PMBT was coated onto the surfaces of polypropylene hollow fiber membrane (PP-HFM) oxygenator and its connecting tubes. The PMBT copolymer coating on the oxygenator circuit formed a cell outer membrane mimetic surface with excellent stability. The hemocompatibility of the PMBT copolymer coated PP-HFM oxygenator circuit was evaluated by animal extracorporeal circulation. The concentrations of clotting components fibrinogen and platelet in the blood were almost unchanged during the circulation through the PMBT copolymer coated oxygenator circuits. By contrast, the concentrations of fibrinogen and platelet were significantly reduced to 52% and 56% respectively in the uncoated oxygenator group due to adsorption and thrombogenesis of the blood during 2h circulation. Moreover, concentration of activation marker beta-thromboglobulin for platelet in the blood was remarkably lower in the PMBT group than the uncoated control group (p<0.01). All the results strongly supported that the hemocompatibility of the PP-HFM oxygenator circuit could be improved significantly by coating a stable and densely assembled zwitterionic polymer film. This simple, stable and highly effective cell membrane mimetic coating strategy may be applicable in developing advanced oxygenator systems and other artificial organs.
  
  
  
  Although a number of studies have reported the fabrication of zwitterionic phosphorylcholine coated oxygenators to resist the adsorption and activation of blood components and eliminate heparin-induced thrombocytopenia, none of them have fabricated stable and densely assembled film, especially with crosslinkable amphiphilic random copolymer described in our manuscript. The novel features of our work include.
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an, Shaanxi 710127, PR China.
authors.forename: Yan-Bing
authors.initials: YB
authors.lastname: Wang
authors.affiliation: Dialysis Department of Nephrology Center, First Affiliated Hospital of Medicine School, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, PR China.
authors.forename: Ke-Hui
authors.initials: KH
authors.lastname: Shi
authors.affiliation: Dialysis Department of Nephrology Center, First Affiliated Hospital of Medicine School, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, PR China. Electronic address: j92106@sina.com.
authors.forename: Hong-Li
authors.initials: HL
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an, Shaanxi 710127, PR China. Electronic address: gongyk@nwu.edu.cn.
authors.forename: Yong-Kuan
authors.initials: YK
authors.lastname: Gong
mesh_terms: D000327:Adsorption; D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D002448:Cell Adhesion; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004285:Dogs; D008297:Male; D008567:Membranes, Artificial; D010107:Oxygenators, Membrane; D010973:Platelet Adhesiveness; D011126:Polypropylenes / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; D011126:Polypropylenes
keywords: Cell membrane mimetic coating; Hemocompatibility; Oxygenator; Phosphorylcholine; Zwitterion