title: Discovery of a Regulatory Motif for Human Satellite DNA Transcription in Response to BATF2 Overexpression.
abstract: BACKGROUND
  One of the basic leucine zipper transcription factors, BATF2, has been found to suppress cancer growth and migration. However, little is known about the genes downstream of BATF2.
  
  
  METHODS
  HeLa cells were stably transfected with BATF2, then chromatin immunoprecipitation-sequencing was employed to identify the DNA motifs responsive to BATF2.
  
  
  RESULTS
  Comprehensive bioinformatics analyses indicated that the most significant motif discovered as TTCCATT[CT]GATTCCATTC[AG]AT was primarily distributed among the chromosome centromere regions and mostly within human type II satellite DNA. Such motifs were able to prime the transcription of type II satellite DNA in a directional and asymmetrical manner. Consistently, satellite II transcription was up-regulated in BATF2-overexpressing cells.
  
  
  CONCLUSIONS
  The present study provides insight into understanding the role of BATF2 in tumours and the importance of satellite DNA in the maintenance of genomic stability.
authors.affiliation: Department of Medical Genetics, Capital Medical University, Beijing, P.R. China.
authors.forename: Xuejia
authors.initials: X
authors.lastname: Bai
authors.affiliation: Department of Biochemistry and Molecular Biology, Capital Medical University, Beijing, P.R. China.
authors.forename: Wenqiu
authors.initials: W
authors.lastname: Huang
authors.affiliation: Department of Biochemistry and Molecular Biology, Capital Medical University, Beijing, P.R. China.
authors.forename: Chenguang
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, Capital Medical University, Beijing, P.R. China weiding@ccmu.edu.cn niujing2006@sina.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Niu
authors.affiliation: Department of Biochemistry and Molecular Biology, Capital Medical University, Beijing, P.R. China Beijing Key Laboratory for Tumor Invasion and Metastasis, Cancer Institute of Capital Medical University, Beijing, P.R. China Beijing Institute of Brain Disorders, Beijing, P.R. China weiding@ccmu.edu.cn niujing2006@sina.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Ding
mesh_terms: D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics* / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly; D047369:Chromatin Immunoprecipitation; D019295:Computational Biology; D004276:DNA, Satellite / Q000235:genetics* / Q000378:metabolism; D005260:Female; D006367:HeLa Cells; D006570:Heterochromatin / Q000235:genetics / Q000378:metabolism; D006801:Humans; D012045:Regulatory Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D013997:Time Factors; D014158:Transcription, Genetic; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; C569485:Batf2 protein, human; D004276:DNA, Satellite; D006570:Heterochromatin; D025521:Tumor Suppressor Proteins
keywords: BATF2/SARI; ChIP-seq; genomic stability; satellite DNA