title: Adhesion of human gingival fibroblasts/Streptococcus mitis co-culture on the nanocomposite system Chitlac-nAg.
abstract: Composite materials are increasingly used as dental restoration. In the field of biomaterials, infections remain the main reason of dental devices failure. Silver, in the form of nanoparticles (AgNPs), ions and salt, well known for its antimicrobial properties, is used in several medical applications in order to avoid bacterial infection. To reduce both bacterial adhesion to dental devices and cytotoxicity against eukaryotic cells, we coated BisGMA/TEGDMA methacrylic thermosets with a new material, Chitlac-nAg, formed by stabilized AgNPs with a polyelectrolyte solution containing Chitlac. Here we analyzed the proliferative and adhesive ability of human gingival fibroblasts (HGFs) on BisGMA/TEGDMA thermosets uncoated and coated with AgNPs in a coculture model system with Streptococcus mitis. After 48 h, HGFs well adhered onto both surfaces, while S. mitis cytotoxic response was higher in the presence of AgNPs coated thermosets. After 24 h thermosets coated with Chitlac as well as those coated with Chitlac-nAg exerted a minimal cytotoxic effect on HGFs, while after 48 h LDH release raised up to 20 %. Moreover the presence of S. mitis reduced this release mainly when HGFs adhered to Chitlac-nAg coated thermosets. The reduced secretion of collagen type I was significant in the presence of both surfaces with the co-culture system even more when saliva is added. Integrin β1 localized closely to cell membranes onto Chitlac-nAg thermosets and PKCα translocated into nuclei. These data confirm that Chitlac-nAg have a promising utilization in the field of restorative dentistry exerting their antimicrobial activity due to AgNPs without cytotoxicity for eukaryotic cells.
authors.affiliation: Department of Pharmacy, G. d'Annunzio University, Chieti-Pescara, Via dei Vestini 31, 66100, Chieti Scalo, CH, Italy.
authors.forename: Amelia
authors.initials: A
authors.lastname: Cataldi
authors.affiliation: Department of Pharmacy, G. d'Annunzio University, Chieti-Pescara, Via dei Vestini 31, 66100, Chieti Scalo, CH, Italy. marialucia.gallorini@unich.it.
authors.forename: Marialucia
authors.initials: M
authors.lastname: Gallorini
authors.affiliation: Department of Pharmacy, G. d'Annunzio University, Chieti-Pescara, Via dei Vestini 31, 66100, Chieti Scalo, CH, Italy.
authors.forename: Mara
authors.initials: M
authors.lastname: Di Giulio
authors.affiliation: Center for Aging Science (Ce.S.I.), G. d'Annunzio University Foundation, Chieti, Italy.|Department of Neuroscience, Imaging and Clinical Sciences, G. d'Annunzio University, Chieti-Pescara, Chieti, Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Guarnieri
authors.affiliation: Center for Aging Science (Ce.S.I.), G. d'Annunzio University Foundation, Chieti, Italy.|Department of Neuroscience, Imaging and Clinical Sciences, G. d'Annunzio University, Chieti-Pescara, Chieti, Italy.
authors.forename: Maria Addolorata
authors.initials: MA
authors.lastname: Mariggiò
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences, G. d'Annunzio University, Chieti-Pescara, Chieti, Italy.
authors.forename: Tonino
authors.initials: T
authors.lastname: Traini
authors.affiliation: Department of Medicine and Ageing Sciences, G. d'Annunzio University, Chieti-Pescara, Chieti, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Di Pietro
authors.affiliation: Department of Pharmacy, G. d'Annunzio University, Chieti-Pescara, Via dei Vestini 31, 66100, Chieti Scalo, CH, Italy.
authors.forename: Luigina
authors.initials: L
authors.lastname: Cellini
authors.affiliation: Department of Medical, Surgical and Health Sciences, University of Trieste, Trieste, Italy.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Marsich
authors.affiliation: Department of Pharmacy, G. d'Annunzio University, Chieti-Pescara, Via dei Vestini 31, 66100, Chieti Scalo, CH, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Sancilio
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology*; D002470:Cell Survival; D018920:Coculture Techniques; D003470:Culture Media; D005347:Fibroblasts / Q000382:microbiology* / Q000502:physiology; D006801:Humans; D008422:Materials Testing; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry*; D012463:Saliva; D034361:Streptococcus mitis / Q000502:physiology*; D013499:Surface Properties
chemical_list: D003470:Culture Media