title: Altered function of monocytes/macrophages in patients with autoimmune hepatitis.
abstract: The pathogenesis of autoimmune hepatitis (AIH) involves the intervention of the innate and adaptive immune responses. In the current study, the alterations in monocytes/Kupffer cells (KCs) were investigated in patients with AIH. A total of 21 patients with AIH at different stages of the disease, and 7 controls with non‑alcoholic fatty liver disease were selected. The abundance of VAV1 and p21‑activated kinase 1 (PAK1) in the liver and KCs was analyzed. In addition, the expression levels of HLA‑DR and CD80 in the peripheral blood monocytes (PBMs) were measured, and phagocytosis of PBMs was assessed. KCs of AIH patients exhibited higher expression levels of VAV1 and PAK1. This upregulated expression was associated with disease progression. A reduced expression of HLA‑DR and CD80, and reduced capacity of E. coli phagocytosis in PBMs was observed for patients with AIH. This downregulated expression was associated with disease progression. The results of the current study indicated that defective function of KCs and PBMs may be involved in the pathogenesis of AIH.
authors.affiliation: Department of Digestive Diseases, General Hospital, Tianjin Medical University, Tianjin 300052, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Lin
authors.affiliation: Department of Digestive Diseases, General Hospital, Tianjin Medical University, Tianjin 300052, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Digestive Diseases, General Hospital, Tianjin Medical University, Tianjin 300052, P.R. China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Digestive Diseases, General Hospital, Tianjin Medical University, Tianjin 300052, P.R. China.
authors.forename: Bangmao
authors.initials: B
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D018122:B7-1 Antigen / Q000276:immunology / Q000378:metabolism; D006684:HLA-DR Antigens / Q000276:immunology / Q000378:metabolism; D019693:Hepatitis, Autoimmune / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007728:Kupffer Cells / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D010587:Phagocytosis; D051567:Proto-Oncogene Proteins c-vav / Q000276:immunology / Q000378:metabolism; D054462:p21-Activated Kinases / Q000276:immunology / Q000378:metabolism
chemical_list: D018122:B7-1 Antigen; D006684:HLA-DR Antigens; D051567:Proto-Oncogene Proteins c-vav; C496618:VAV1 protein, human; C515903:PAK1 protein, human; D054462:p21-Activated Kinases