title: Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes.
abstract: We describe organelle thermometry using an endoplasmic reticulum-targeting small molecule dye and cytosolic mCherry, whose fluorescence lifetimes reduce with increasing temperature and can be monitored by fluorescence lifetime imaging microscopy. The results show that heat production in single myotubes is highly localized and is coupled to a Ca(2+) burst.
authors.affiliation: Department of Pure and Applied Physics, Graduate School of Advanced Science and Engineering, Waseda University, Tokyo 169-8555, Japan and Institute of Medical Biology, Agency for Science, Technology and Research (A*STAR), Singapore 138648, Singapore. birgit.lane@imb.a-star.edu.sg.
authors.forename: Hideki
authors.initials: H
authors.lastname: Itoh
authors.affiliation: Organization for University Research Initiatives, Waseda University, Tokyo 162-0041, Japan. suzu_mado@aoni.waseda.jp Ishiwata@waseda.jp and Waseda Bioscience Research Institute in Singapore (WABIOS), Singapore 138667, Singapore.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Arai
authors.affiliation: Institute of Medical Biology, Agency for Science, Technology and Research (A*STAR), Singapore 138648, Singapore. birgit.lane@imb.a-star.edu.sg.
authors.forename: Thankiah
authors.initials: T
authors.lastname: Sudhaharan
authors.affiliation: Aptabio Therapeutics Inc., Yongin 446-908, Korea.
authors.forename: Sung-Chan
authors.initials: SC
authors.lastname: Lee
authors.affiliation: Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), Singapore 138667, Singapore and Department of Chemistry and MedChem Program of Life Sciences Institute, National University of Singapore, Singapore 117543, Singapore.
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Chang
authors.affiliation: Organization for University Research Initiatives, Waseda University, Tokyo 162-0041, Japan. suzu_mado@aoni.waseda.jp Ishiwata@waseda.jp and Waseda Bioscience Research Institute in Singapore (WABIOS), Singapore 138667, Singapore and Department of Physics, Faculty of Advanced Science and Engineering, Waseda University, Tokyo 169-8555, Japan.
authors.forename: Shin'ichi
authors.initials: S
authors.lastname: Ishiwata
authors.affiliation: Organization for University Research Initiatives, Waseda University, Tokyo 162-0041, Japan. suzu_mado@aoni.waseda.jp Ishiwata@waseda.jp and Waseda Bioscience Research Institute in Singapore (WABIOS), Singapore 138667, Singapore.
authors.forename: Madoka
authors.initials: M
authors.lastname: Suzuki
authors.affiliation: Institute of Medical Biology, Agency for Science, Technology and Research (A*STAR), Singapore 138648, Singapore. birgit.lane@imb.a-star.edu.sg.
authors.forename: E Birgitte
authors.initials: EB
authors.lastname: Lane