title: Lipase coated clusters of iron oxide nanoparticles for biodiesel synthesis in a solvent free medium.
abstract: Methyl or ethyl esters of long chain fatty acids are called biodiesel. Biodiesel is synthesized by the alcoholysis of oils/fats. In this work, lipase from Thermomyces lanuginosus was precipitated over the clusters of Fe3O4 nanoparticles. This biocatalyst preparation was used for obtaining biodiesel from soybean oil. After optimization of both immobilization conditions and process parameters, complete conversion to biodiesel was obtained in 3h and on lowering the enzyme amount, as little as 1.7U of lipase gave 96% conversion in 7h. The solvent free media with oil:ethanol (w/w) of 1:4 and 40°C with 2% (w/w) water along with 20% (w/w) silica (for facilitating acyl migration) were employed for reaching this high % of conversion. The biocatalyst design enables one to use a rather small amount of lipase. This should help in switching over to a biobased production of biodiesel.
authors.affiliation: Department of Chemistry, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India.
authors.forename: Joyeeta
authors.initials: J
authors.lastname: Mukherjee
authors.affiliation: Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India. Electronic address: munishwar48@yahoo.co.uk.
authors.forename: Munishwar Nath
authors.initials: MN
authors.lastname: Gupta
mesh_terms: D001203:Ascomycota / Q000201:enzymology; D055162:Biocatalysis; D056804:Biofuels; D003246:Conservation of Energy Resources; D004800:Enzymes, Immobilized; D004952:Esters; D000431:Ethanol; D005227:Fatty Acids; D005290:Ferric Compounds / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D053758:Nanoparticles; D013024:Soybean Oil / Q000737:chemistry
chemical_list: D056804:Biofuels; D004800:Enzymes, Immobilized; D004952:Esters; D005227:Fatty Acids; D005290:Ferric Compounds; C000499:ferric oxide; D000431:Ethanol; D013024:Soybean Oil; D008049:Lipase
keywords: Biodiesel; Bioimprinting; Lipases; Magnetic nanoparticles; Transesterification