title: Colocalization of Granulysin Protein Forms with Perforin and LAMP-1 in Decidual Lymphocytes During Early Pregnancy.
abstract: Granulysin (GNLY) occurs in two forms, which have molecular weights of 9 and 15 kDa. We analyzed the cytotoxic potential of decidual lymphocytes (DLs) and peripheral blood lymphocytes (PBLs) based on the forms of GNLY that colocalizes with perforin (PER) and LAMP-1 following activation.
  
  
  
  The forms of GNLY were detected by using confocal microscopy. We investigated the colocalization with PER and LAMP-1 in freshly isolated and activated DLs and PBLs.
  
  
  
  Activation of DLs and PBLs by K-562 cells increased the colocalization of 9 kDa GNLY with PER and LAMP-1. K-562 cells transfected with HLA-C decreased 9 kDa GNLY colocalization with PER in DLs only. IL-15 in DLs decreased 9 kDa GNLY and LAMP-1 colocalization, but increased both 15 kDa GNLY and LAMP-1, and PER and LAMP-1 colocalization.
  
  
  
  Activated DLs and PBLs show greater cytotoxic potential based on increased colocalization of 9 kDa GNLY and PER. HLA-C and IL-15 affect DLs, indicating their role in maintaining the pregnancy tolerance.
authors.affiliation: Department of Physiology and Immunology, Medical Faculty, University of Rijeka, Rijeka, Croatia.
authors.forename: Marin
authors.initials: M
authors.lastname: Dominovic
authors.affiliation: Department of Physiology and Immunology, Medical Faculty, University of Rijeka, Rijeka, Croatia.|Division of Cardiology, Hospital for Medical Rehabilitation of the Hearth and Lung Diseases and Rheumatism "Thalassotherapia" Opatija, Opatija, Croatia.
authors.forename: Gordana
authors.initials: G
authors.lastname: Laskarin
authors.affiliation: Department of Obstetrics and Gynecology, Clinical Hospital Center Rijeka, Rijeka, Croatia.
authors.forename: Lana
authors.initials: L
authors.lastname: Glavan Gacanin
authors.affiliation: Department of Obstetrics and Gynecology, Clinical Hospital Center Rijeka, Rijeka, Croatia.
authors.forename: Herman
authors.initials: H
authors.lastname: Haller
authors.affiliation: Department of Physiology and Immunology, Medical Faculty, University of Rijeka, Rijeka, Croatia.|Department of Clinical and Transplantation Immunology and Molecular Medicine in Rijeka, Croatian Academy of Sciences and Arts, Rijeka, Croatia.
authors.forename: Daniel
authors.initials: D
authors.lastname: Rukavina
mesh_terms: D000328:Adult; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism*; D003602:Cytotoxicity, Immunologic; D003656:Decidua / Q000276:immunology*; D005260:Female; D006801:Humans; D020014:K562 Cells; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D051907:Lysosomal Membrane Proteins / Q000378:metabolism*; D054353:Perforin / Q000378:metabolism*; D011247:Pregnancy; D021381:Protein Transport; D055815:Young Adult; D052118:Lysosomal-Associated Membrane Protein 1
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D051907:Lysosomal Membrane Proteins; D054353:Perforin; C075005:GNLY protein, human; C489116:LAMP1 protein, human; D052118:Lysosomal-Associated Membrane Protein 1
keywords: Granulysin; lymphocytes; perforin; pregnancy