title: Color-Coded Super-Resolution Small-Molecule Imaging.
abstract: Although the development of super-resolution microscopy dates back to 1994, its applications have been primarily focused on visualizing cellular structures and targets, including proteins, DNA and sugars. We now report on a system that allows both monitoring of the localization of exogenous small molecules in live cells at low resolution and subsequent super-resolution imaging by using stochastic optical reconstruction microscopy (STORM) on fixed cells. This represents a powerful new tool to understand the dynamics of subcellular trafficking associated with the mode and mechanism of action of exogenous small molecules.
authors.affiliation: Waitt Advanced Biophotonics Center, The Salk Institute for Biological Sciences, 10010 N Torrey Pines Road, La Jolla, CA, 92037, USA.
authors.forename: Paolo
authors.initials: P
authors.lastname: Beuzer
authors.affiliation: Waitt Advanced Biophotonics Center, The Salk Institute for Biological Sciences, 10010 N Torrey Pines Road, La Jolla, CA, 92037, USA. i@xenobe.org.|Xenobe Research Institute, P. O. Box 3052, San Diego, CA, 92163-1052, USA. i@xenobe.org.
authors.forename: James J
authors.identifier: 0000-0001-6500-4107
authors.initials: JJ
authors.lastname: La Clair
authors.affiliation: Waitt Advanced Biophotonics Center, The Salk Institute for Biological Sciences, 10010 N Torrey Pines Road, La Jolla, CA, 92037, USA. hucang@salk.edu.
authors.forename: Hu
authors.initials: H
authors.lastname: Cang
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000378:metabolism; D002232:Carbocyanines / Q000737:chemistry; D045744:Cell Line, Tumor; D003116:Color; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008856:Microscopy, Fluorescence; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D054852:Small Molecule Libraries / Q000737:chemistry*
chemical_list: C569686:Alexa Fluor 647; D000911:Antibodies, Monoclonal; D002232:Carbocyanines; D011506:Proteins; D054852:Small Molecule Libraries; D004247:DNA
keywords: drug discovery; microscopy; mode of action; natural products; super-resolution imaging