title: Comparison of Serum Spec fPL(™) and 1,2-o-Dilauryl-Rac-Glycero-3-Glutaric Acid-(6'-Methylresorufin) Ester Assay in 60 Cats Using Standardized Assessment of Pancreatic Histology.
abstract: BACKGROUND
  Feline pancreas-specific lipase (Spec fPL) is considered a useful test for the antemortem diagnosis of pancreatitis in cats. A recent study found good agreement between the results of the Spec fPL and catalytic 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6'-methylresorufin) ester (DGGR) lipase assay. Prospective studies evaluating their sensitivity and specificity are lacking.
  
  
  OBJECTIVE
  To compare the results of the Spec fPL and the DGGR assays with a standardized histologic assessment of the pancreas.
  
  
  METHODS
  Sixty client-owned cats presented for necropsy.
  
  
  METHODS
  
  
  
  METHODS
  Spec fPL concentrations and serum DGGR lipase activity were measured from the same blood sample. The pancreas was removed within 3 hours after euthanasia; serial transverse sections were made every 0.5 cm throughout the entire pancreas and reviewed using a histologic grading scheme. Sensitivity and specificity for the Spec fPL and DGGR assay results were determined.
  
  
  RESULTS
  The sensitivity and specificity for the Spec fPL assay (cutoff value ≥5.4 μg/L) was 42.1 [95% confidence interval (95% CI), 29.4-55.9%] and 100% (95% CI, 31.0-100.0%). The sensitivity and specificity for the DGGR assay (cutoff value >26 U/L) was 36.8 (95% CI, 24.7-50.7%) and 100% (95% CI, 31.0-100.0%). When lymphocytic inflammation up to 10% of a section was considered normal, the sensitivity and specificity for Spec fPL assay (cutoff value ≥5.4 μg/L) was 61.1 (95% CI, 36.1-81.7%) and 69.0% (95% CI, 52.8-81.9%) and the sensitivity and specificity for the DGGR assay (cutoff value >26 U/L) was 66.7 (95% CI, 41.2-85.6%) and 78.6% (95% CI, 62.8-89.2%).
  
  
  CONCLUSIONS
  Both lipase assays performed similarly well, but their agreement with histologic pancreatic inflammation was limited.
authors.affiliation: Clinic for Small Animal Internal Medicine, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
authors.forename: S
authors.identifier: http://orcid.org/0000-0002-3815-8589
authors.initials: S
authors.lastname: Oppliger
authors.affiliation: Institute for Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Hilbe
authors.affiliation: Section of Epidemiology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
authors.forename: S
authors.initials: S
authors.lastname: Hartnack
authors.affiliation: Clinic for Small Animal Internal Medicine, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.|Department of Animal Medicine, Production and Health, University of Padova, Legnaro, PD, Italy.|Istituto Veterinario di Novara, Granozzo con Monticello, NO, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Zini
authors.affiliation: Clinic for Small Animal Internal Medicine, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
authors.forename: C E
authors.initials: CE
authors.lastname: Reusch
authors.affiliation: Clinic for Small Animal Internal Medicine, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
authors.forename: P H
authors.initials: PH
authors.lastname: Kook
mesh_terms: D000818:Animals; D053099:Azotemia / Q000097:blood / Q000662:veterinary*; D001681:Biological Assay / Q000662:veterinary; D002371:Cat Diseases / Q000097:blood* / Q000175:diagnosis / Q000201:enzymology; D002415:Cats; D005260:Female; D005977:Glutarates; D008049:Lipase / Q000097:blood*; D008297:Male; D010078:Oxazines; D010179:Pancreas / Q000201:enzymology / Q000473:pathology*; D010195:Pancreatitis / Q000175:diagnosis / Q000201:enzymology / Q000662:veterinary*; D012680:Sensitivity and Specificity
chemical_list: C501502:1,2-o-dilauryl-rac-glycero glutaric acid-(6'-methyl resorufin) ester; D005977:Glutarates; D010078:Oxazines; D008049:Lipase
keywords: Feline; Feline pancreas-specific lipase; Histopathology; Lipase; Pancreas