title: Recombinant endo-mannanase (ManB-1601) production using agro-industrial residues: Development of economical medium and application in oil extraction from copra.
abstract: Expression of pRSETA manb-1601 construct in Hi-Control Escherichia coli BL21 (DE3) cells improved recombinant endo-mannanase (ManB-1601) production by 2.73-fold (1821±100U/ml). A low-cost, agro-industrial residue supplemented industrial medium for enhanced and economical production of ManB-1601 was developed in two mutual phases. Phase-I revealed the potential of various pre- (induction time: 5h, induction mode: lactose 0.5mM) and post-induction [peptone supplementation: 0.94%(w/v), glycerol 0.123%(v/v)] parameters for enhanced production of ManB-1601 and resulted in 4.61-fold (8406±400U/ml) and 2.53-fold (3.30g/l) higher ManB-1601 and biomass production, respectively. Under phase-II, economization of phase-I medium was carried out by reducing/replacing costly ingredients with solubilized-defatted flax seed meal (S-DFSM), which resulted in 3.25-fold (5926U/ml) higher ManB-1601 production. Industrial potential of ManB-1601 was shown in oil extraction from copra as enzyme treatment led to cracks, peeling, fracturing and smoothening of copra, which facilitated higher (18.75%) oil yield.
authors.affiliation: Department of Protein Chemistry and Technology, CSIR-Central Food Technological Research Institute, Mysuru 570 020, India; Academy of Scientific and Innovative Research (AcSIR), CSIR-CFTRI Campus, Mysuru, India.
authors.forename: Gaurav Singh
authors.initials: GS
authors.lastname: Kaira
authors.affiliation: Department of Protein Chemistry and Technology, CSIR-Central Food Technological Research Institute, Mysuru 570 020, India; Academy of Scientific and Innovative Research (AcSIR), CSIR-CFTRI Campus, Mysuru, India.
authors.forename: Deepesh
authors.initials: D
authors.lastname: Panwar
authors.affiliation: Department of Protein Chemistry and Technology, CSIR-Central Food Technological Research Institute, Mysuru 570 020, India; Academy of Scientific and Innovative Research (AcSIR), CSIR-CFTRI Campus, Mysuru, India. Electronic address: mkapoor@cftri.res.in.
authors.forename: Mukesh
authors.initials: M
authors.lastname: Kapoor
mesh_terms: D018533:Biomass; D000074263:Coconut Oil; D003059:Cocos / Q000737:chemistry* / Q000187:drug effects; D003470:Culture Media; D004926:Escherichia coli / Q000378:metabolism*; D008361:Mannosidases / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D010938:Plant Oils / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000494:pharmacology
chemical_list: D003470:Culture Media; D010938:Plant Oils; D011994:Recombinant Proteins; D008361:Mannosidases; D000074263:Coconut Oil
keywords: Copra oil extraction; Endo-mannanase; Flax seed meal; Industrial medium; Process optimization