title: First report of novel genetic array aacA4-blaIMP-25-oxa30-catB3 and identification of novel metallo-β-lactamase gene blaIMP25: A Retrospective Study of antibiotic resistance surveillance on Psuedomonas aeruginosa in Guangzhou of South China, 2003-2007.
abstract: Carbapenem, imipenem and meropenem have been broadly prescribed contributing to the global occurrence and prevalence of carbapenem resistance in Psuedomonas aeruginosa, and the associated resistance genotypes remains clinically significant. A retrospective surveillance had been conducted on 499 P. aeruginosa isolates in South China during 2003-2007, including antimicrobial resistance and characterization of MBLs on carbapenem-resistant strains. One hundred and sixty-four out of 499 strains were carbapenem-resistant, with 11, 4 and 5 strains positive for blaIMP-9, blaIMP-25 and blaVIM-2, respectively. Sixteen out of 20 isolates were positive for intI1 and contained identical flanking regions (as indicated in KM384735), and all tested isolates containing the qacE△1-sul1 of the typical 3'-conserved region. A novel blaIMP-25 metallo-β-lactamase and a genetic array of aacA4-blaIMP-25-oxa30-catB3 have been discovered from this retrospective surveillance on antimicrobial resistance of P. aeruginosa.
authors.affiliation: Clinical Laboratory Center, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China.
authors.forename: Guangchao
authors.initials: G
authors.lastname: Yu
authors.affiliation: Clinical Laboratory Center, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China.
authors.forename: Wangrong
authors.initials: W
authors.lastname: Wen
authors.affiliation: Department of Clinical Pharmacy, College of Pharmacy, University of Tennessee Health Sciences Center, Memphis, TN 38163, USA.
authors.forename: Brian M
authors.initials: BM
authors.lastname: Peters
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors.forename: Junyan
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Dermatology and Venerology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Congxiu
authors.initials: C
authors.lastname: Ye
authors.affiliation: Clinical Laboratory Center, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China.
authors.forename: Yuchuan
authors.initials: Y
authors.lastname: Che
authors.affiliation: Clinical Laboratory Center, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China.
authors.forename: Juzhen
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Microbiology, Zhongshan Medical School of Sun Yat-sen University, Guangzhou 510080, China. Electronic address: caoky@mail.sysu.edu.cn.
authors.forename: Kaiyuan
authors.initials: K
authors.lastname: Cao
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China; Department of Microbial Pathogenesis, School of Dentistry, University of Maryland, Baltimore, MD, USA. Electronic address: zhenbo.xu@hotmail.com.
authors.forename: Zhenbo
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Microbial Pathogenesis, School of Dentistry, University of Maryland, Baltimore, MD, USA.
authors.forename: Mark E
authors.initials: ME
authors.lastname: Shirtliff
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015780:Carbapenems / Q000494:pharmacology; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial; D062665:Epidemiological Monitoring; D023061:Gene Order; D005798:Genes, Bacterial; D006801:Humans; D032023:Integrons; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010802:Phylogeny; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics; D012189:Retrospective Studies; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D004269:DNA, Bacterial; D001618:beta-Lactamases
keywords: Carbapenem-resistant; Metallo-β-lactamase; P. aeruginosa; aacA4-blaIMP-25-oxa30-catB3; bla(IMP-25)