title: The Helicobacter pylori Cag Pathogenicity Island Protein Cag1 is Associated with the Function of T4SS.
abstract: The human pathogen Helicobacter pylori is involved in gastric diseases ranging from gastritis to gastric cancer. Virulent strains harboring the cag pathogenicity island (cag PAI) which encode a Type IV Secretion System (T4SS) can induce pro-inflammatory cytokines such as interleukin-8 and deliver their major effector proteins CagA into the gastric cells. While a subset of cag PAI genes have been identified to be the homologues of T4SS genes from Agrobacterium tumefaciens, a majority have unknown functions. We have identified one of such proteins, Cag1, which was predicted to be a non-classically secreted and virulent protein. Our results showed that Cag1 is a membrane-associated protein essential for the induction of multiple cytokine secretions, and cag1-deficient mutant has partial influence on CagA translocation; while the protein itself was not injected into host cells. Our data indicated that Cag1 is located in the bacterial membrane and is associated with the function of T4SS.
authors.affiliation: Department of Pathogenic Biology, School of Medical Science, Jiangsu University, Zhenjiang, 212013, Jiangsu, China.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, Kunshan Hospital of Traditional Chinese Medicine, Kunshan, 215300, Jiangsu, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Ling
authors.affiliation: Department of Pathogenic Biology, School of Medical Science, Jiangsu University, Zhenjiang, 212013, Jiangsu, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Pathogenic Biology, School of Medical Science, Jiangsu University, Zhenjiang, 212013, Jiangsu, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yu
authors.affiliation: Department of Pathogenic Biology, School of Medical Science, Jiangsu University, Zhenjiang, 212013, Jiangsu, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Pathogenic Biology, School of Medical Science, Jiangsu University, Zhenjiang, 212013, Jiangsu, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Pathogenic Biology, School of Medical Science, Jiangsu University, Zhenjiang, 212013, Jiangsu, China.
authors.forename: Jingyi
authors.initials: J
authors.lastname: Qian
authors.affiliation: Department of Pathogenic Biology, School of Medical Science, Jiangsu University, Zhenjiang, 212013, Jiangsu, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Pathogenic Biology, School of Medical Science, Jiangsu University, Zhenjiang, 212013, Jiangsu, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Pathogenic Biology, School of Medical Science, Jiangsu University, Zhenjiang, 212013, Jiangsu, China. shaoshihe2006@163.com.
authors.forename: Shihe
authors.initials: S
authors.lastname: Shao
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D044404:Genomic Islands; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D021381:Protein Transport; D000069319:Type IV Secretion Systems / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; C103937:PicB protein, Helicobacter pylori; D000069319:Type IV Secretion Systems