title: A new cytotoxic diterpenoid glycoside from the leaves of Blumea lacera and its effects on apoptosis and cell cycle.
abstract: A new diterpenoid glycoside, 6E,10E,14Z-(3S)-17-hydroxygeranyllinalool-17-O-β-d-glucopyranosyl-(1 → 2)-[α-l-rhamnopyranosyl-(1 → 6)]-β-d-glucopyranoside (1) together with the known diterpenoid glycoside (2) and two known flavonoid glycosides (3, 4) were isolated from the methanol extract of Blumea lacera leaves. The structures were determined by the interpretation of their spectroscopic data and comparison with the literature. All compounds were isolated for the first time from B. lacera and evaluated for their cytotoxic activity. Only the new compound (1) showed strong cytotoxic activity with the lowest IC50value (8.3 μM) being displayed against MCF-7 breast cancer cells. In apoptosis and cell cycle analysis, 1 revealed strong apoptotic activity against MCF-7 cells (45.5% AV+/PI-) after 24 h, but showed no arresting of any of the cell cycle phases in MCF-7.
authors.affiliation: a School of Pharmacy , Griffith University , Queensland , Australia.|b Department of Pharmacy , BRAC University , Dhaka , Bangladesh.
authors.forename: Raushanara
authors.initials: R
authors.lastname: Akter
authors.affiliation: a School of Pharmacy , Griffith University , Queensland , Australia.|c Pharmacy Discipline , Khulna University , Khulna , Bangladesh.
authors.forename: Shaikh J
authors.initials: SJ
authors.lastname: Uddin
authors.affiliation: d Institute for Glycomics , Griffith University , Queensland , Australia.
authors.forename: Joe
authors.initials: J
authors.lastname: Tiralongo
authors.affiliation: d Institute for Glycomics , Griffith University , Queensland , Australia.|e School of Medical Science , Griffith University , Queensland , Australia.
authors.forename: I Darren
authors.initials: ID
authors.lastname: Grice
authors.affiliation: a School of Pharmacy , Griffith University , Queensland , Australia.|f Menzies Health Institute Queensland , Griffith University , Queensland , Australia.
authors.forename: Evelin
authors.initials: E
authors.lastname: Tiralongo
keywords:  leaves; apoptosis; cell cycle analysis; cytotoxicity; diterpenoid glycoside; flavonoid glycoside