title: Chemerin as a Novel Crevicular Fluid Marker of Patients With Periodontitis and Type 2 Diabetes Mellitus.
abstract: The objectives of the present study are to: 1) determine whether gingival crevicular fluid (GCF) chemerin is a novel predictive marker for patients with chronic periodontitis (CP) with and without type 2 diabetes mellitus (t2DM); 2) analyze the relationship between chemerin and interleukin (IL)-6 in periodontally healthy individuals and in patients with CP and with and without t2DM; and 3) evaluate the effect of non-surgical periodontal therapy on GCF chemerin levels.
  
  
  
  Eighty individuals were split into four groups: 20 who were systemically and periodontally healthy (CTRL), 20 with t2DM and periodontally healthy (DM-CTRL), 20 systemically healthy with CP (CP), and 20 with CP and t2DM (DM-CP). Individuals with periodontitis were treated with non-surgical periodontal therapy. GCF sampling procedures and clinical periodontal measures were performed before and 6 weeks after treatment. Enzyme-linked immunosorbent assay was used to measure chemerin and IL-6 levels.
  
  
  
  Greater values for GCF chemerin and IL-6 levels were found in CP groups than in periodontally healthy groups, in DM-CP than in CP, and in DM-CTRL than in CTRL (P <0.008). GCF chemerin and IL-6 levels decreased following therapy in CP groups (P <0.02). A comprehensive overview of all groups showed a statistically significant positive correlation of chemerin with IL-6, glycated hemoglobin, sampled-site clinical attachment level, and gingival index (P <0.05).
  
  
  
  In this study, periodontitis and t2DM induced aberrant secretion of chemerin, and non-surgical periodontal therapy influenced the decrease of GCF chemerin levels in patients with CP with and without t2DM. Furthermore, it suggests GCF chemerin levels may be considered a potential proinflammatory marker for diabetes, periodontal disease, and treatment outcomes.
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Bülent Ecevit University, Zonguldak, Turkey.
authors.forename: Şeyma Bozkurt
authors.initials: ŞB
authors.lastname: Doğan
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Bülent Ecevit University, Zonguldak, Turkey.
authors.forename: Umut
authors.initials: U
authors.lastname: Ballı
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Bülent Ecevit University, Zonguldak, Turkey.
authors.forename: Figen Öngöz
authors.initials: FÖ
authors.lastname: Dede
authors.affiliation: Department of Medical Biochemistry, Elazig Military Hospital, Elazığ, Turkey.
authors.forename: Erdim
authors.initials: E
authors.lastname: Sertoğlu
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Bülent Ecevit University, Zonguldak, Turkey.
authors.forename: Kaan
authors.initials: K
authors.lastname: Tazegül
mesh_terms: D015415:Biomarkers; D016022:Case-Control Studies; D018925:Chemokines / Q000032:analysis*; D055113:Chronic Periodontitis / Q000150:complications*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications*; D005883:Gingival Crevicular Fluid / Q000737:chemistry; D006801:Humans; D007249:Inflammation; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis*; D015850:Interleukin-6 / Q000032:analysis; D010512:Periodontal Index
chemical_list: D015415:Biomarkers; D018925:Chemokines; D036341:Intercellular Signaling Peptides and Proteins; D015850:Interleukin-6; C478630:RARRES2 protein, human
keywords: Chemerin protein, human; diabetes mellitus; gingival crevicular fluid; interleukin-6; periodontal disease; periodontitis