title: Spatial-Temporal Changes of Bacterioplankton Community along an Exhorheic River.
abstract: To date, few aquatic microbial ecology studies have discussed the variability of the microbial community in exorheic river ecosystems on both the spatial and seasonal scales. In this study, we examined the spatio-temporal variation of bacterioplankton community composition in an anthropogenically influenced exorheic river, the Haihe River in Tianjin, China, using pyrosequencing analysis of 16S rRNA genes. It was verified by one-way ANOVA that the spatial variability of the bacterioplankton community composition over the whole river was stronger than the seasonal variation. Salinity was a major factor leading to spatial differentiation of the microbial community structure into riverine and estuarial parts. A high temperature influence on the seasonal bacterial community variation was only apparent within certain kinds of environments (e.g., the riverine part). Bacterial community richness and diversity both exhibited significant spatial changes, and their seasonal variations were completely different in the two environments studied here. Furthermore, riverine bacterial community assemblages were subdivided into urban and rural groups due to changes in the nutritional state of the river. In addition, the nutrient-loving group including Limnohabitans, Hydrogenophaga, and Polynucleobacter were abundant in the urbanized Haihe River, indicating the environmental factors in these anthropogenic waterbodies heavily influence the core freshwater community composition.
authors.affiliation: Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai UniversityTianjin, China; Department of Environmental Engineering and Safety Engineering, College of Chemistry and Chemical Engineering, Southwest Petroleum UniversityChengdu, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Ma
authors.affiliation: Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University Tianjin, China.
authors.forename: Guannan
authors.initials: G
authors.lastname: Mao
authors.affiliation: Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University Tianjin, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University Tianjin, China.
authors.forename: Guanghai
authors.initials: G
authors.lastname: Gao
authors.affiliation: LPMC and Institute of Statistics, Nankai University Tianjin, China.
authors.forename: Changliang
authors.initials: C
authors.lastname: Zou
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University Tianjin, China.
authors.forename: Mark G
authors.initials: MG
authors.lastname: Bartlam
authors.affiliation: Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University Tianjin, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Wang
keywords: bacterial diversity; bacterioplankton community; seasonal pattern; spatial pattern