title: Comparative analysis of chemical constituents, antimicrobial and antioxidant activities of ethylacetate extracts of Polygonum cuspidatum and its endophytic actinomycete, Streptomyces sp. A0916.
abstract: The present study investigated the chemical composition of ethylacetate extracts from an endophytic actinomycete Streptomyces sp. A0916 and its host Polygonum cuspidatum. A comparative analysis of the antimicrobial and antioxidant properties of the extracts was also conducted. 32 compounds of P. cuspidatum and 23 compounds of Streptomyces sp. A0916 were isolated and identified by GC/MS. Antimicrobial activities of the extracts were evaluated using eight microbial strains (3 Gram-positive bacteria, 3 Gram-negative bacteria, and 2 fungi). The Streptomyces sp. A0916 extracts showed a wide range of antimicrobial activities and presented greater antimicrobial effectiveness than the P. cuspidatum extracts. The minimum inhibitory concentration (MIC) of Streptomyces sp. A0916 extracts against the ampicillin-resistant strain Enterococcus faecium SIIA843 was 32 μg·mL(-1). Furthermore, the extracts had greater antimicrobial effect against Gram-positive bacteria than Gram-negative bacteria. Finally, the antioxidant activity of the Streptomyces sp. A0916 extracts was equal to that of the P. cuspidatum extracts. In conclusion, our results suggest that the endophytic actinomycetes of the medicinal plants are an important source of bioactive substances.
authors.affiliation: Key laboratory of Leather Chemistry and engineering, Ministry of Education and College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu 610065, China; Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key laboratory of Leather Chemistry and engineering, Ministry of Education and College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu 610065, China; Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Qiu
authors.affiliation: Key laboratory of Leather Chemistry and engineering, Ministry of Education and College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu 610065, China.
authors.forename: Xiu-Feng
authors.initials: XF
authors.lastname: Long
authors.affiliation: Key laboratory of Leather Chemistry and engineering, Ministry of Education and College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu 610065, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Sichuan Industrial Institute of Antibiotics, Chengdu 610052, China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Zeng
authors.affiliation: Key laboratory of Leather Chemistry and engineering, Ministry of Education and College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu 610065, China; Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, China. Electronic address: yqtian@scu.edu.cn.
authors.forename: Yong-Qiang
authors.initials: YQ
authors.lastname: Tian
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D045468:Fallopia japonica / Q000737:chemistry* / Q000382:microbiology*; D005658:Fungi / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D013302:Streptomyces / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000302:isolation & purification
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D010936:Plant Extracts
keywords: Antimicrobial activity; Antioxidant activity; Endophytic actinomycete; Ethylacetate extracts; GC/MS; Polygonum cuspidatum