title: Susceptibility and molecular characterization of Candida species from patients with vulvovaginitis.
abstract: Vulvovaginal candidiasis affects women of reproductive age, which represents approximately 15-25% of vaginitis cases. The present study aimed to isolate and characterize yeast from the patients irrespective of the presentation of clinical symptoms. The isolates were subjected to in vitro susceptibility profile and characterization by molecular markers, which intended to assess the distribution of species. A total of 40 isolates were obtained and identified through the CHROMagar, API20aux and by ITS and D1/D2 regions sequencing of DNAr gene. Candida albicans strains were genotyped by the ABC system and the isolates were divided into two genotypic groups. The identity of the C. albicans, C. glabrata, C. guilliermondii, C. kefyr and Saccharomyces cerevisiae isolates was confirmed by the multilocus analysis. The strains of Candida, isolated from patients with complications, were found to be resistant to nystatin but sensitive to fluconazole, amphotericin B and ketoconazole, as observed by in vitro sensitivity profile. The isolates from asymptomatic patients, i.e., the colonized group, showed a dose-dependent sensitivity to the anti-fungal agents, fluconazole and amphotericin B. However, the isolates of C. albicans that belong to distinct genotypic groups showed the same in vitro susceptibility profile.
authors.affiliation: Graduate Program in Microbiology, Parasitology and Pathology, Department of Basic Pathology, Laboratory of Microbiology and Molecular Biology-LabMicro, Federal University of Paraná, Curitiba, Paraná, Brazil.
authors.forename: Gheniffer
authors.initials: G
authors.lastname: Fornari
authors.affiliation: Graduate Program in Microbiology, Parasitology and Pathology, Department of Basic Pathology, Laboratory of Microbiology and Molecular Biology-LabMicro, Federal University of Paraná, Curitiba, Paraná, Brazil.
authors.forename: Vania Aparecida
authors.initials: VA
authors.lastname: Vicente
authors.affiliation: Graduate Program in Microbiology, Parasitology and Pathology, Department of Basic Pathology, Laboratory of Microbiology and Molecular Biology-LabMicro, Federal University of Paraná, Curitiba, Paraná, Brazil.
authors.forename: Renata Rodrigues
authors.initials: RR
authors.lastname: Gomes
authors.affiliation: Support and Diagnosis Unit, Mycology Laboratory, Federal University of Paraná, Brazil.
authors.forename: Marisol Dominguez
authors.initials: MD
authors.lastname: Muro
authors.affiliation: Support and Diagnosis Unit, Mycology Laboratory, Federal University of Paraná, Brazil.
authors.forename: Rosangela Lameira
authors.initials: RL
authors.lastname: Pinheiro
authors.affiliation: Clinical Hospital Federal University of Paraná, Brazil.
authors.forename: Carolina
authors.initials: C
authors.lastname: Ferrari
authors.affiliation: Graduate Program in Microbiology, Parasitology and Pathology, Department of Basic Pathology, Laboratory of Microbiology and Molecular Biology-LabMicro, Federal University of Paraná, Curitiba, Paraná, Brazil.
authors.forename: Patricia Fernanda
authors.initials: PF
authors.lastname: Herkert
authors.affiliation: Clinical Hospital Federal University of Paraná, Brazil.
authors.forename: Marcos
authors.initials: M
authors.lastname: Takimura
authors.affiliation: Clinical Hospital Federal University of Paraná, Brazil.
authors.forename: Newton Sérgio de
authors.initials: NS
authors.lastname: Carvalho
authors.affiliation: Graduate Program in Microbiology, Parasitology and Pathology, Department of Basic Pathology, Laboratory of Microbiology and Molecular Biology-LabMicro, Federal University of Paraná, Curitiba, Paraná, Brazil; Support and Diagnosis Unit, Mycology Laboratory, Federal University of Paraná, Brazil. Electronic address: Queiroz.telles@uol.com.br.
authors.forename: Flavio
authors.initials: F
authors.lastname: Queiroz-Telles
mesh_terms: D000293:Adolescent; D000328:Adult; D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D002181:Candidiasis, Vulvovaginal / Q000382:microbiology*; D025141:Drug Resistance, Fungal; D005260:Female; D015725:Fluconazole / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D010361:Patients / Q000706:statistics & numerical data; D055815:Young Adult
chemical_list: D000935:Antifungal Agents; D015725:Fluconazole
keywords: In vitro susceptibility; Variability genetic; Vulvovaginal candidiasis