title: SIRT1, 2, 3 protect mouse oocytes from postovulatory aging.
abstract: The quality of metaphase II oocytes will undergo a time-dependent deterioration following ovulation as the result of the oocyte aging process. In this study, we determined that the expression of sirtuin family members (SIRT1, 2, 3) was dramatically reduced in mouse oocytes aged in vivo or in vitro. Increased intracellular ROS was observed when SIRT1, 2, 3 activity was inhibited. Increased frequency of spindle defects and disturbed distribution of mitochondria were also observed in MII oocytes aged in vitro after treatment with Nicotinamide (NAM), indicating that inhibition of SIRT1, 2, 3 may accelerate postovulatory oocyte aging. Interestingly, when MII oocytes were exposed to caffeine, the decline of SIRT1, 2, 3 mRNA levels was delayed and the aging-associated defective phenotypes could be improved. The results suggest that the SIRT1, 2, 3 pathway may play a potential protective role against postovulatory oocyte aging by controlling ROS generation.
authors.affiliation: Institute of Reproductive Sciences, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.|State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Teng
authors.initials: T
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.|Department of Reproductive Medicine, Guangdong Women and Children Hospital, Guangzhou, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Institute of Reproductive Sciences, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.|State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Hong-Hui
authors.initials: HH
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Xue-Shan
authors.initials: XS
authors.lastname: Ma
authors.affiliation: Department of Reproductive Medicine, Peking University Shenzhen Hospital, Medical Center of Peking University, Shenzhen, Guangdong, China.
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Qian
authors.affiliation: Institute of Reproductive Sciences, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
authors.affiliation: Department of Veterinary Pathobiology, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Heide
authors.initials: H
authors.lastname: Schatten
authors.affiliation: Institute of Reproductive Sciences, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.|State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Sun
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D016922:Cellular Senescence / Q000187:drug effects / Q000502:physiology*; D005260:Female; D051379:Mice; D009536:Niacinamide / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D056564:Sirtuin 1 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D056565:Sirtuin 2 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D056566:Sirtuin 3 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008941:Spindle Apparatus / Q000187:drug effects / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D009536:Niacinamide; D056564:Sirtuin 1; D056565:Sirtuin 2; D056566:Sirtuin 3
keywords: SIRT1, 2, 3; caffeine; nicotinamide; postovulatory aging