title: Ferulone A and ferulone B: two new coumarin esters from Ferula orientalis L. roots.
abstract: Ferula orientalis (Apiaceae) is a well-known perennial herb growing wild in Iran used in traditional medicine. To perform phytochemical studies, dried ground roots of F. orientalis were sequentially Soxhlet-extracted using n-hexane, dichloromethane and methanol. A combination of vacuum liquid chromatography and preparative thin-layer chromatographic analyses were performed to isolate coumarin esters. The structures of the isolated compounds were elucidated by spectroscopic means, and in vitro free-radical-scavenging property was determined by the DPPH assay. Two new coumarin esters, 7-O-(4,8,12,16-tetrahydroxy-4,8,12,16-tetramethyl-heptadecanoyl)-coumarinand 7-O-(4-hydroxy-4,8,12-trimethyl-trideca-7,11-dienoyl)-coumarin, named ferulone A and ferulone B, respectively, were isolated from the n-hexane extract of the roots of F. orientalis. Both compounds showed a low level of free-radical-scavenging property with the RC50 values of 0.252 and 0.556 mg/mL for compounds 1 and 2, respectively, as opposed to that of the positive control (quercetin) 0.004 mg/mL. This is the first report on the purification of coumarin esters from the genus Ferula.
authors.affiliation: a Faculty of Sciences, Department of Biology , University of Mohaghegh Ardabili , Ardabil , Iran.
authors.forename: Seyed Mehdi
authors.initials: SM
authors.lastname: Razavi
authors.affiliation: b School of Pharmacy and Biomolecular Sciences, Medicinal Chemistry and Natural Products Research Group , Liverpool John Moores University , Liverpool , UK.
authors.forename: Lutfun
authors.initials: L
authors.lastname: Nahar
authors.affiliation: a Faculty of Sciences, Department of Biology , University of Mohaghegh Ardabili , Ardabil , Iran.
authors.forename: Hamideh
authors.initials: H
authors.lastname: Talischi
authors.affiliation: b School of Pharmacy and Biomolecular Sciences, Medicinal Chemistry and Natural Products Research Group , Liverpool John Moores University , Liverpool , UK.
authors.forename: Satyajit Dey
authors.initials: SD
authors.lastname: Sarker
mesh_terms: D005591:Chemical Fractionation; D002855:Chromatography, Thin Layer; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004952:Esters / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005233:Fatty Alcohols / Q000302:isolation & purification* / Q000494:pharmacology; D005309:Ferula / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D006586:Hexanes / Q000737:chemistry; D007492:Iran; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry
chemical_list: D003374:Coumarins; D004952:Esters; D005233:Fatty Alcohols; D016166:Free Radical Scavengers; D006586:Hexanes; D010936:Plant Extracts; C000619191:ferulone A; C000619192:ferulone B; C026385:n-hexane
keywords: DPPH; Ferula orientalis; ferulone; free-radical-scavenging; umbelliferone