title: Ring finger protein 10 is a novel synaptonuclear messenger encoding activation of NMDA receptors in hippocampus.
abstract: Synapses and nuclei are connected by bidirectional communication mechanisms that enable information transfer encoded by macromolecules. Here, we identified RNF10 as a novel synaptonuclear protein messenger. RNF10 is activated by calcium signals at the postsynaptic compartment and elicits discrete changes at the transcriptional level. RNF10 is enriched at the excitatory synapse where it associates with the GluN2A subunit of NMDA receptors (NMDARs). Activation of synaptic GluN2A-containing NMDARs and induction of long term potentiation (LTP) lead to the translocation of RNF10 from dendritic segments and dendritic spines to the nucleus. In particular, we provide evidence for importin-dependent long-distance transport from synapto-dendritic compartments to the nucleus. Notably, RNF10 silencing prevents the maintenance of LTP as well as LTP-dependent structural modifications of dendritic spines.
authors.affiliation: Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Milano, Italy.
authors.forename: Margarita C
authors.initials: MC
authors.lastname: Dinamarca
authors.affiliation: Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Milano, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Guzzetti
authors.affiliation: RG Neuroplasticity, Leibniz Institute for Neurobiology, Magdeburg, Germany.
authors.forename: Anna
authors.initials: A
authors.lastname: Karpova
authors.affiliation: Dipartimento di Scienze del Farmaco, Università degli Studi del Piemonte Orientale "Amedeo Avogadro", Novara, Italy.
authors.forename: Dmitry
authors.initials: D
authors.lastname: Lim
authors.affiliation: Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Milano, Italy.
authors.forename: Nico
authors.initials: N
authors.lastname: Mitro
authors.affiliation: Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Milano, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Musardo
authors.affiliation: Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Milano, Italy.
authors.forename: Manuela
authors.initials: M
authors.lastname: Mellone
authors.affiliation: Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Milano, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Marcello
authors.affiliation: Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Milano, Italy.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Stanic
authors.affiliation: Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Milano, Italy.
authors.forename: Tanmoy
authors.initials: T
authors.lastname: Samaddar
authors.affiliation: CNRS, Institut de Génomique Fonctionnelle, Montpellier, France.
authors.forename: Adeline
authors.initials: A
authors.lastname: Burguière
authors.affiliation: Dipartimento di Scienze del Farmaco, Università degli Studi del Piemonte Orientale "Amedeo Avogadro", Novara, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Caldarelli
authors.affiliation: Dipartimento di Scienze del Farmaco, Università degli Studi del Piemonte Orientale "Amedeo Avogadro", Novara, Italy.
authors.forename: Armando A
authors.initials: AA
authors.lastname: Genazzani
authors.affiliation: CNRS, Institut de Génomique Fonctionnelle, Montpellier, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Perroy
authors.affiliation: CNRS, Institut de Génomique Fonctionnelle, Montpellier, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Fagni
authors.affiliation: Dipartimento di Scienze del Farmaco, Università degli Studi del Piemonte Orientale "Amedeo Avogadro", Novara, Italy.
authors.forename: Pier Luigi
authors.initials: PL
authors.lastname: Canonico
authors.affiliation: RG Neuroplasticity, Leibniz Institute for Neurobiology, Magdeburg, Germany.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Kreutz
authors.affiliation: Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Milano, Italy.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Gardoni
authors.affiliation: Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Milano, Italy.
authors.forename: Monica
authors.identifier: http://orcid.org/0000-0003-2298-615X
authors.initials: M
authors.lastname: Di Luca
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D006624:Hippocampus / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D021381:Protein Transport; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D013569:Synapses / Q000502:physiology*
chemical_list: D002352:Carrier Proteins; D009419:Nerve Tissue Proteins; C000612391:RNF10 protein, rat; D016194:Receptors, N-Methyl-D-Aspartate
keywords: NMDA receptors; dendritic spines; hippocampus; importins; neuroscience; rat; rattus; synaptonuclear messengers