title: Overexpression of the truncated version of ILV2 enhances glycerol production in Saccharomyces cerevisiae.
abstract: Acetolactate synthase is a mitochondrial enzyme that catalyses the conversion of two pyruvate molecules to an acetolactate molecule with release of carbon dioxide. The overexpression of the truncated version of the corresponding gene, ILV2, that codes for presumably cytosolic acetolactate synthase in the yeast Saccharomyces cerevisiae, led to a decrease in intracellular pyruvate concentration. This recombinant strain was also characterized by a four-fold increase in glycerol production, with a concomitant 1.8-fold reduction in ethanol production, when compared to that of the wild-type strain under anaerobic conditions in a glucose alcoholic fermentation. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, NAS of Ukraine, Lviv, Ukraine.
authors.forename: Lidiia
authors.initials: L
authors.lastname: Murashchenko
authors.affiliation: Archer Daniels Midland Co Research Center, 1001 N. Brush College Road, Decatur, IL, USA.
authors.forename: Charles
authors.initials: C
authors.lastname: Abbas
authors.affiliation: Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, NAS of Ukraine, Lviv, Ukraine.
authors.forename: Kostyantyn
authors.initials: K
authors.lastname: Dmytruk
authors.affiliation: Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, NAS of Ukraine, Lviv, Ukraine.|Department of Biotechnology and Microbiology, University of Rzeszow, Poland.
authors.forename: Andriy
authors.initials: A
authors.lastname: Sibirny
mesh_terms: D019342:Acetic Acid / Q000032:analysis / Q000378:metabolism; D000095:Acetolactate Synthase / Q000235:genetics / Q000378:metabolism*; D000431:Ethanol / Q000032:analysis / Q000378:metabolism; D005285:Fermentation; D015870:Gene Expression; D005800:Genes, Fungal; D005947:Glucose / Q000032:analysis / Q000378:metabolism; D005990:Glycerol / Q000032:analysis / Q000378:metabolism*; D060847:Metabolic Engineering; D008928:Mitochondria / Q000201:enzymology*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D000431:Ethanol; D000095:Acetolactate Synthase; D005947:Glucose; D005990:Glycerol; D019342:Acetic Acid
keywords: Saccharomyces cerevisiae; alcoholic fermentation; ethanol; glycerol; metabolic engineering; pyruvate