title: Valorisation of untreated cane molasses for enhanced phytase production by Bacillus subtilis K46b and its potential role in dephytinisation.
abstract: BACKGROUND
  The high cost of phytase production is the most limiting factor in its application in animal feeds. The present study aimed to develop a low-cost medium for production of a novel phytase in submerged fermentation using inexpensive agro-industrial by-products. The applicability of phytase in dephytinisation of commonly used food/feed ingredients, i.e. soybean meal and wheat bran, was also investigated.
  
  
  RESULTS
  Using a one-factor-at-a-time approach, soybean meal and cane molasses were identified as significant agro-industrial by-products and these factors were subsequently optimised using response surface methodology (RSM). A central composite design was employed to further enhance phytase yield. Under optimum conditions of soybean meal 22.3 g L-1, cane molasses 100 g L-1and 39 h fermentation, phytase production increased to 56.562 U mL-1, indicating more than 28-fold enhancement. The enzyme efficiently dephytinised wheat bran and soybean meal after 24 h incubation at 56.5 °C and increased inorganic phosphate content by 240% and 155%, respectively.
  
  
  CONCLUSIONS
  Soybean meal and cane molasses were successfully used for enhancement of phytase production as economical carbon, nitrogen and phytic acid sources using RSM. The phytase showed a good capability to dephytinise wheat bran and soybean meal, demonstrating that the enzyme can be considered as a potential candidate for industrial food and feed applications. © 2016 Society of Chemical Industry.
authors.affiliation: Department of Food Science, Engineering and Technology, Faculty of Agricultural Engineering and Technology, University of Tehran, P.O. Box 4111, 31587-77871, Karaj, Iran.|Microbial Biotechnology Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research Education and Extension Organization (AREEO), P.O. Box 3135933151, 31535-1897, Karaj, Iran.
authors.forename: Karim
authors.initials: K
authors.lastname: Rocky-Salimi
authors.affiliation: Microbial Biotechnology Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research Education and Extension Organization (AREEO), P.O. Box 3135933151, 31535-1897, Karaj, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Hashemi
authors.affiliation: Department of Food Science, Engineering and Technology, Faculty of Agricultural Engineering and Technology, University of Tehran, P.O. Box 4111, 31587-77871, Karaj, Iran.|Center of Excellence for Application of Modern Technology for Producing Functional Foods and Drinks, University of Tehran, P.O. Box 4111, 31587-77871, Karaj, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Safari
authors.affiliation: Microbial Biotechnology Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research Education and Extension Organization (AREEO), P.O. Box 3135933151, 31535-1897, Karaj, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Mousivand
mesh_terms: D010832:6-Phytase / Q000096:biosynthesis*; D000383:Agriculture; D000821:Animal Feed; D001412:Bacillus subtilis / Q000201:enzymology* / Q000378:metabolism; D004043:Dietary Fiber / Q000032:analysis; D005285:Fermentation; D005511:Food Handling / Q000379:methods*; D007220:Industrial Waste; D008965:Molasses; D010833:Phytic Acid / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry
chemical_list: D004043:Dietary Fiber; D007220:Industrial Waste; D010833:Phytic Acid; D010832:6-Phytase
keywords: cane molasses; dephytinisation; phytase; response surface methodology; soybean meal; wheat bran