title: The effect of anaerobic-aerobic and feast-famine cultivation pattern on bacterial diversity during poly-β-hydroxybutyrate production from domestic sewage sludge.
abstract: The main objective of this work was to investigate the influence of different oxygen supply patterns on poly-β-hydroxybutyrate (PHB) yield and bacterial community diversity. The anaerobic-aerobic (A/O) sequencing batch reactors (SBR1) and feast-famine (F/F) SBR2 were used to cultivate activated sludge to produce PHB. The mixed microbial communities were collected and analyzed after 3 months cultivation. The PHB maximum yield was 64 wt% in SBR1 and 53 wt% in SBR2. Pyrosequencing analysis 16S rRNA gene of two microbial communities indicated there were nine and four bacterial phyla in SBR1 and SBR2, respectively. Specifically, Proteobacteria (36.4 % of the total bacterial community), Actinobacteria (19.7 %), Acidobacteria (14.1 %), Firmicutes (4.4 %), Bacteroidetes (1.7 %), Cyanobacteria/Chloroplast (1.5 %), TM7 (0.8 %), Gemmatimonadetes (0.2 %), and Nitrospirae (0.1 %) were present in SBR1. Proteobacteria (94.2 %), Bacteroidetes (2.9 %), Firmicutes (1.9 %), and Actinobacteria (0.7 %) were present in SBR2. Our results indicated the SBR1 fermentation system was more stable than that of SBR2 for PHB accumulation.
authors.affiliation: College of Life Sciences, Northeast Forestry University, Harbin, 150040, China.
authors.forename: Changli
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Energy, Environmental and Chemical Engineering, Washington University, St. Louis, MO, 63130, USA.
authors.forename: Di
authors.initials: D
authors.lastname: Liu
authors.affiliation: College of Mechanical and Electrical Engineering, Northeast Forestry University, Harbin, 150040, China.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Qi
authors.affiliation: Key laboratory of forest plant ecology, ministry of education, Northeast Forestry University, Harbin, 150040, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Forestry, Northeast Forestry University, Harbin, 150040, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Northeast Forestry University, Harbin, 150040, China. 704828816@qq.com.
authors.forename: Min
authors.initials: M
authors.lastname: Zhao
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D006885:Hydroxybutyrates / Q000378:metabolism*; D011091:Polyesters / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*; D000094122:Polyhydroxybutyrates
chemical_list: D006885:Hydroxybutyrates; D011091:Polyesters; D012336:RNA, Ribosomal, 16S; D012722:Sewage; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
keywords: Anaerobic–aerobic; Bacterial diversity; Feast–famine; Poly-β-hydroxybutyrate; Sequencing batch reactors