title: [The reduced level of antibodies to acetylcholine receptor alpha 7 fragment in the blood serum of patients with Alzheimer's disease].
abstract: OBJECTIVE
  Determination of antibodies to neuronal membrane proteins in the blood serum of patients is of interest for diagnosis and optimization of treatment of Alzheimer's disease (AD). Authors studied the level of antibodies to acetylcholine receptor alpha 7 protein fragment (AChR), prion protein (РrР) and glycation end-products (RAGE) as well as to intracellular proteins nucleophosmin (Nuc) and survivin (Sur) in the serum of AD patients.
  
  
  METHODS
  Serum samples of 26 patients with probable AD and 13 healthy people were studied. Exposed sections of each protein were used for the choice of peptides for antibody visualization. ELIZA was a main method in this study.
  
  
  CONCLUSIONS
  Antibodies to several proteins were identified but significant differences were found only for AChR-(173-193). The results demonstrated the involvement of AChR and AChR-antibodies in the development of AD. Determination of antibodies to AChR-(173-193) may be a marker of AD and a method for specifying the diagnosis of AD.
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
authors.forename: A V
authors.initials: AV
authors.lastname: Kamynina
authors.affiliation: Research Center of Mental Health, Moscow.
authors.forename: E V
authors.initials: EV
authors.lastname: Ponomareva
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
authors.forename: D O
authors.initials: DO
authors.lastname: Koroev
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
authors.forename: T D
authors.initials: TD
authors.lastname: Volkova
authors.affiliation: Research Center of Mental Health, Moscow.
authors.forename: I V
authors.initials: IV
authors.lastname: Kolykhalov
authors.affiliation: Research Center of Mental Health, Moscow.
authors.forename: N D
authors.initials: ND
authors.lastname: Selezneva
authors.affiliation: Research Center of Mental Health, Moscow.
authors.forename: S I
authors.initials: SI
authors.lastname: Gavrilova
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
authors.forename: O M
authors.initials: OM
authors.lastname: Vol'pina
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000097:blood / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000276:immunology; D064569:alpha7 Nicotinic Acetylcholine Receptor / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D015415:Biomarkers; D010446:Peptide Fragments; D064569:alpha7 Nicotinic Acetylcholine Receptor