title: Chemical composition and in vitro evaluation of antioxidant, antimicrobial, cytotoxicity and anti-acetylcholinesterase properties of Tunisian Origanum majorana L. essential oil.
abstract: This study investigated the chemical composition and evaluated the antioxidant, antimicrobial, cytotoxic and anti-acetylcholinesterase properties of Tunisian Origanum majorana essential oil. The findings showed that the oil exhibited high activity, particularly in terms of reducing power and β-Carotene bleaching, inducing higher IC50 values than BHT. The oil showed an important antimicrobial activity against 25 bacterial and fungal strains. In fact, the IZ, MIC and MBC values recorded for the bacterial strains were in the range of 8 ± 0-18.33 ± 0.57 mm, 0.097-3.125 and 0.39-6.25 mg/mL, respectively. The IZ, MIC and MFC values of the fungal strains varied between 11±0-28 ± 0 mm, 0.058-0.468 mg/mL and 0.234-1.875 mg/mL, respectively. A low cytotoxic effect was observed against cancer (Hep-2 and HT29) and continuous cell lineage (Vero), with CC50 values ranging from 13.73 to 85.63 mg/mL. The oil was also evaluated for anti-acetylcholinesterase effects, which showed that it exhibited significant activity with IC50 values reaching 150.33 ± 2.02 μg/mL.
authors.affiliation: Laboratory of Transmissible Diseases and Biologically Active Substances, Department of Microbiology, Faculty of Pharmacy, University of Monastir, Avicenne Street, 5000 Monastir, Tunisia. Electronic address: bio.hafedh@gmail.com.
authors.forename: Hafedh
authors.initials: H
authors.lastname: Hajlaoui
authors.affiliation: Arid Lands Institute, Range Ecology Laboratory, 4119 Medenine, Tunisia.
authors.forename: Hedi
authors.initials: H
authors.lastname: Mighri
authors.affiliation: Laboratory of Transmissible Diseases and Biologically Active Substances, Department of Microbiology, Faculty of Pharmacy, University of Monastir, Avicenne Street, 5000 Monastir, Tunisia.
authors.forename: Mahjoub
authors.initials: M
authors.lastname: Aouni
authors.affiliation: Laboratory of Plant Biotechnology, Faculty of Science, University of Sfax, B.P. 1171, 3000, Tunisia.
authors.forename: Néji
authors.initials: N
authors.lastname: Gharsallah
authors.affiliation: Laboratory of Plant Biotechnology, Faculty of Science, University of Sfax, B.P. 1171, 3000, Tunisia; College of Science and Arts in Baljurashi, Al Baha University, P.O.Box (1988) Al Baha, Saudi Arabia.
authors.forename: Adel
authors.initials: A
authors.lastname: Kadri
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005658:Fungi / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D031341:Origanum / Q000737:chemistry*; D010084:Oxidation-Reduction; D064209:Phytochemicals / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D019207:beta Carotene / Q000378:metabolism
chemical_list: D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D000975:Antioxidants; D009822:Oils, Volatile; D064209:Phytochemicals; D019207:beta Carotene; D000110:Acetylcholinesterase
keywords: Anti acetylcholinesterase; Antimicrobial; Antioxidant; Cytotoxicity; Essential oil; Origanum majorana