title: Protection of donor lung inflation in the setting of cold ischemia against ischemia-reperfusion injury with carbon monoxide, hydrogen, or both in rats.
abstract: OBJECTIVE
  Lung ischemia-reperfusion injury (IRI) may be attenuated through carbon monoxide (CO)'s anti-inflammatory effect or hydrogen (H2)'s anti-oxidant effect. In this study, the effects of lung inflation with CO, H2, or both during the cold ischemia phase on graft function were observed.
  
  
  METHODS
  Rat donor lungs, inflated with 40% oxygen (control group), 500ppm CO (CO group), 3% H2 (H2 group) or 500ppm CO+3% H2 (COH group), were kept at 4°C for 180min. After transplantation, the recipients' artery blood gas and pressure-volume (P-V) curves were analyzed. The inflammatory response, oxidative stress and apoptosis in the recipients were assessed at 180min after reperfusion.
  
  
  RESULTS
  Oxygenation in the CO and H2 groups were improved compared with the control group. The CO and H2 groups also exhibited significantly improved P-V curves, reduced lung injury, and decreased inflammatory response, malonaldehyde content, and cell apoptosis in the grafts. Furthermore, the COH group experienced enhanced improvements in oxygenation, P-V curves, inflammatory response, lipid peroxidation, and graft apoptosis compared to the CO and H2 groups.
  
  
  CONCLUSIONS
  Lung inflation with CO or H2 protected against IRI via anti-inflammatory, anti-oxidant and anti-apoptotic mechanisms in a model of lung transplantation in rats, which was enhanced by combined treatment with CO and H2.
authors.affiliation: Department of Anesthesiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Hei Longjiang Province 150001, China; The Hei Longjiang Province Key Lab of Research on Anesthesiology and Critical Care Medicine, Harbin, Hei Longjiang Province 150001, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Meng
authors.affiliation: Department of Dermatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Hei Longjiang Province 150001, China.
authors.forename: Liangjuan
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Anesthesiology, The 211 Hospital of the Chinese People's Liberation Army, Harbin, Hei Longjiang Province 150001, China.
authors.forename: Li
authors.initials: L
authors.lastname: Niu
authors.affiliation: Department of Anesthesiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Hei Longjiang Province 150001, China; The Hei Longjiang Province Key Lab of Research on Anesthesiology and Critical Care Medicine, Harbin, Hei Longjiang Province 150001, China.
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Cui
authors.affiliation: Department of Anesthesiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Hei Longjiang Province 150001, China; The Hei Longjiang Province Key Lab of Research on Anesthesiology and Critical Care Medicine, Harbin, Hei Longjiang Province 150001, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, Hei Longjiang Province 150001, China.
authors.forename: Jiyu
authors.initials: J
authors.lastname: Kang
authors.affiliation: Department of Anesthesiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Hei Longjiang Province 150001, China; The Hei Longjiang Province Key Lab of Research on Anesthesiology and Critical Care Medicine, Harbin, Hei Longjiang Province 150001, China.
authors.forename: Rongfang
authors.initials: R
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Hei Longjiang Province 150001, China; The Hei Longjiang Province Key Lab of Research on Anesthesiology and Critical Care Medicine, Harbin, Hei Longjiang Province 150001, China.
authors.forename: Jingchun
authors.initials: J
authors.lastname: Xing
authors.affiliation: Department of Anesthesiology, The General Hospital of Daqing Oilfield, Daqing, Hei Longjiang Province 163000, China.
authors.forename: Changlin
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Department of Anesthesiology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, Hei Longjiang Province 150001, China. Electronic address: zhouhuacheng@163.com.
authors.forename: Huacheng
authors.initials: H
authors.lastname: Zhou
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001784:Blood Gas Analysis; D002248:Carbon Monoxide / Q000494:pharmacology*; D050377:Cold Ischemia / Q000379:methods*; D004357:Drug Synergism; D006859:Hydrogen / Q000494:pharmacology*; D018836:Inflammation Mediators / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D055370:Lung Injury / Q000517:prevention & control*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D015427:Reperfusion Injury / Q000517:prevention & control*; D019737:Transplants / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D018836:Inflammation Mediators; D002248:Carbon Monoxide; D006859:Hydrogen
keywords: Carbon monoxide; Cold ischemia phase; Hydrogen; Ischemia-reperfusion injury; Lung inflation; Lung transplantation