title: Study on the Synthesis, Characterization and Bioactivities of 3-Methyl-9'-fluorenespiro-5-hydantoin.
abstract: This work describes a method for synthesis, as well as in vitro antiproliferative and antibacterial investigation of 3-methyl-9'-fluorenespiro-5-hydantoin. The structure of the substituted fluorenylspirohydantoin derivative was verified by UV-Vis, FT-IR, Raman, (1)H-NMR and (13)C-NMR spectroscopy, and by using a combination of 2D NMR experiments, which included (1)H-(1)H COSY, HMQC and HMBC sequences. The geometry of the compound was optimized by the B3LYP density functional with 6-31G(d) basis set and the (1)H and (13)C NMR spectra were predicted with the HF/6-31G(d) calculations at the optimized geometry. The anticancer activity of the 3-methyl-9'-fluorenespiro-5-hydantoin was determined in suspension cell lines originating from tumors in humans (WERI-Rb-1). The cytotoxic effect was evaluated by WST-assay (Roche Applied Science). The antimicrobial effect of the compound against Gram-negative, Gram-positive bacteria and the yeast Candida albicans was investigated.
authors.forename: Petja
authors.initials: P
authors.lastname: Marinova
authors.forename: Marin
authors.initials: M
authors.lastname: Marinov
authors.forename: Maria
authors.initials: M
authors.lastname: Kazakova
authors.forename: Yana
authors.initials: Y
authors.lastname: Feodorova
authors.forename: Alexander
authors.initials: A
authors.lastname: Slavchev
authors.forename: Denica
authors.initials: D
authors.lastname: Blazheva
authors.forename: Danail
authors.initials: D
authors.lastname: Georgiev
authors.forename: Plamen
authors.initials: P
authors.lastname: Penchev
authors.forename: Victoria
authors.initials: V
authors.lastname: Sarafian
authors.forename: Neyko
authors.initials: N
authors.lastname: Stoyanov
mesh_terms: D000890:Anti-Infective Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000138:chemical synthesis*; D045744:Cell Line, Tumor; D006801:Humans; D006827:Hydantoins / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy
chemical_list: D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D006827:Hydantoins