title: Effective Strategy for Conformer-Selective Detection of Short-Lived Excited State Species: Application to the IR Spectroscopy of the N1H Keto Tautomer of Guanine.
abstract: The ultrafast deactivation processes in the excited state of biomolecules, such as the most stable tautomers of guanine, forbid any state-of-the-art gas phase spectroscopic studies on these species with nanosecond lasers. This drawback can be overcome by grafting a chromophore having a long-lived excited state to the molecule of interest, allowing thus a mass-selective detection by nanosecond R2PI and therefore double resonance IR/UV conformer-selective spectroscopic studies. The principle is presently demonstrated on the keto form of a modified 9-methylguanine, for which the IR/UV double resonance spectrum in the C═O stretch region, reported for the first time, provides evidence for extensive vibrational couplings within the guanine moiety. Such a successful strategy opens up a route to mass-selective IR/UV spectroscopic investigations on molecules exhibiting natural chromophores having ultrashort-lived excited states, such as DNA bases, their complexes as well as peptides containing short-lived aromatic residues.
authors.affiliation: Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology , 4259-J2-12, Nagatsuta-cho Midori, Yokohama 226-8501, Japan.|LIDYL, CEA, CNRS, Université Paris-Saclay , CEA Saclay, 91191 Gif-sur-Yvette, France.
authors.forename: Hiroya
authors.initials: H
authors.lastname: Asami
authors.affiliation: Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology , 4259-J2-12, Nagatsuta-cho Midori, Yokohama 226-8501, Japan.
authors.forename: Munefumi
authors.initials: M
authors.lastname: Tokugawa
authors.affiliation: Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology , 4259-J2-12, Nagatsuta-cho Midori, Yokohama 226-8501, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Masaki
authors.affiliation: Laboratory for Chemistry and Life Science, Tokyo Institute of Technology , 4259-R1-15, Nagatsuta-cho Midori, Yokohama 226-8503, Japan.
authors.forename: Shun-Ichi
authors.initials: S
authors.lastname: Ishiuchi
authors.affiliation: LIDYL, CEA, CNRS, Université Paris-Saclay , CEA Saclay, 91191 Gif-sur-Yvette, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Gloaguen
authors.affiliation: Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology , 4259-J2-12, Nagatsuta-cho Midori, Yokohama 226-8501, Japan.
authors.forename: Kohji
authors.initials: K
authors.lastname: Seio
authors.affiliation: Graduate School of Bio- and Nanosystem Science, Yokohama City University , Yokohama 236-0027, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Saigusa
authors.affiliation: Laboratory for Chemistry and Life Science, Tokyo Institute of Technology , 4259-R1-15, Nagatsuta-cho Midori, Yokohama 226-8503, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Fujii
authors.affiliation: Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology , 4259-J2-12, Nagatsuta-cho Midori, Yokohama 226-8501, Japan.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sekine
authors.affiliation: LIDYL, CEA, CNRS, Université Paris-Saclay , CEA Saclay, 91191 Gif-sur-Yvette, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Mons
mesh_terms: D006147:Guanine / Q000031:analogs & derivatives* / Q000737:chemistry*; D011789:Quantum Theory; D013055:Spectrophotometry, Infrared
chemical_list: D006147:Guanine