title: UFLC-MS/MS analysis of four tanshinone components in Salvia miltiorrhizae after ultrasound-assisted extraction.
abstract: A sensitive and rapid ultra-fast liquid chromatography with tandem mass spectrometry (UFLC-MS/MS) method was developed for simultaneous qualitative and quantitative of four characteristic tanshione components including tanshinone IIA, cryptotanshinone, tanshinone I and dihydrotanshinone I in Salvia miltiorrhizae after ultrasound-assisted extraction. By using a C18 column, the four analytes were separated by gradient elution with acetonitrile and water both containing 0.1% formic acid at the flow rate of 0.3mL/min. Multiple-reaction monitoring (MRM) was used for quantification, and an information-dependent acquisition (IDA) method was used to trigger enhanced product ion scans (EPI) for supplementary characteristic identification for qualitative research. Calibration curves showed good linearities with correlation coefficients (r) higher than 0.9990. The method showed high sensitivity with limits of detection (LODs) and quantification (LOQs) less than 0.0002ng/mL and 0.0008ng/mL, respectively, as well as good precision and reproducibility. Mean recoveries for four analytes ranged from 92.5% to 106.2% with relative standard deviations (RSDs) lower than 14.59%. Real application of the developed method in 32 batches of S. miltiorrhizae samples demonstrated that the total contents of four analytes in all samples were in the range of 2.258-52.342mg/g. Ultrasound-assisted extraction technique took a small amount of sample and low time but giving high extraction efficiency. Combining with UFLC-MS/MS method in MRM-IDA-EPI mode, more components in other complicated matrices can be simultaneously analyzed for qualitation and quantitation in one run.
authors.affiliation: Pharmacy College, Liaoning Medical University, Jinzhou 121001, China; Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193 China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Pharmacy College, Liaoning Medical University, Jinzhou 121001, China; Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193 China.
authors.forename: Qiutao
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Luo
authors.affiliation: Guizhou Xinbang Pharmaceutical Co. Ltd., Guiyang 550014,China.
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.affiliation: Guizhou Xinbang Pharmaceutical Co. Ltd., Guiyang 550014,China.
authors.forename: Xiaoru
authors.initials: X
authors.lastname: Wu
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193 China.
authors.forename: Meihua
authors.initials: M
authors.lastname: Yang
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193 China. Electronic address: kongwj302@126.com.
authors.forename: Weijun
authors.initials: W
authors.lastname: Kong
authors.affiliation: Pharmacy College, Liaoning Medical University, Jinzhou 121001, China. Electronic address: wyinguo@163.com.
authors.forename: Weiying
authors.initials: W
authors.lastname: Guo
mesh_terms: D045784:Abietanes / Q000032:analysis*; D002138:Calibration; D057230:Limit of Detection; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014465:Ultrasonics
chemical_list: D045784:Abietanes; C021751:tanshinone
keywords: MRM-IDA-EPI; Salvia miltiorrhizae; Tanshinone; UFLC–MS/MS; Ultrasound-assisted extraction