title: Electrochemical detection of protein glycosylation using lectin and protein-gold affinity interactions.
abstract: We report a new method for the electrochemical detection of glycosylation on proteins, which relies on lectin-protein interaction on a bare gold electrode. The target protein isolated by immunoaffinity is directly adsorbed onto a gold surface and its glycosylation status is retrieved by subsequent addition of specific lectins. The adsorption and subsequent recognition process is monitored electrochemically in the presence of [Fe(CN)6](3-/4-) redox system. By decoupling target protein capture from glycosylation read-out steps, this approach circumvents unwanted antibody-lectin crosstalk while enabling specific glycosylation detection of a glycoprotein in serum-spiked samples in less than 1 h.
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, QLD 4072, Australia. lgcarrascosa@uq.edu.au m.shiddiky@griffith.edu.au m.trau@uq.edu.au.
authors.forename: Sharda
authors.initials: S
authors.lastname: Yadav
authors.forename: Laura G
authors.initials: LG
authors.lastname: Carrascosa
authors.forename: Abu A I
authors.initials: AA
authors.lastname: Sina
authors.forename: Muhammad J A
authors.initials: MJ
authors.lastname: Shiddiky
authors.forename: Michelle M
authors.initials: MM
authors.lastname: Hill
authors.forename: Matt
authors.initials: M
authors.lastname: Trau
mesh_terms: D000327:Adsorption; D000818:Animals; D004563:Electrochemistry / Q000379:methods*; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism*; D006031:Glycosylation; D006046:Gold / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry*; D010047:Ovalbumin / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding
chemical_list: D006023:Glycoproteins; D037102:Lectins; D006046:Gold; D010047:Ovalbumin