title: A Glimpse beyond Structures in Auxin-Dependent Transcription.
abstract: Auxin response factors (ARFs), transcription factors (TFs), and their Aux/IAA (IAA) repressors are central components of the auxin signalling pathway. They interact as homo- and heteromultimers. The structure of their interacting domains revealed a PB1 fold mediating electrostatic interactions through positive and negative faces. Detailed structural analysis revealed additional hydrophobic and polar determinants and started unveiling an ARF/IAA interaction code. Structural progress also shed new light on the DNA binding mode of ARFs showing how they dimerize to bind repeated DNA elements. Here, we discuss the in vitro and in vivo significance of these structural properties for the ARF family of TFs and identify some critical missing information on how specificity might be achieved in the auxin signalling pathway.
authors.affiliation: Laboratoire de Physiologie Cellulaire et Végétale, Université Grenoble Alpes, CNRS UMR5168, CEA/DRF/BIG, INRA UMR 1417, Grenoble, France. Electronic address: francois.parcy@cea.fr.
authors.forename: François
authors.initials: F
authors.lastname: Parcy
authors.affiliation: Laboratoire de Reproduction et Développement des Plantes, CNRS, INRA, ENS de Lyon, UCBL, Université de Lyon, 46 Allée d'Italie, F-69342 Lyon, France. Electronic address: teva.vernoux@ens-lyon.fr.
authors.forename: Teva
authors.initials: T
authors.lastname: Vernoux
authors.affiliation: Laboratoire de Physiologie Cellulaire et Végétale, Université Grenoble Alpes, CNRS UMR5168, CEA/DRF/BIG, INRA UMR 1417, Grenoble, France.
authors.forename: Renaud
authors.initials: R
authors.lastname: Dumas
mesh_terms: D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D007210:Indoleacetic Acids / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D007210:Indoleacetic Acids; D010940:Plant Proteins; D014157:Transcription Factors
keywords: ARF; Aux/IAA; DNA binding; PB1; auxin; oligomerization; structural studies