title: Developmental regulation of chicken surfactant protein A and its localization in lung.
abstract: Surfactant Protein A (SP-A) is a collagenous C-type lectin (collectin) that plays an important role in the early stage of the host immune response. In chicken, SP-A (cSP-A) is expressed as a 26 kDa glycosylated protein in the lung. Using immunohistochemistry, cSP-A protein was detected mainly in the lung lining fluid covering the parabronchial epithelia. Specific cSP-A producing epithelial cells, resembling mammalian type II cells, were identified in the parabronchi. Gene expression of cSP-A markedly increased from embryonic day 14 onwards until the time of hatch, comparable to the SP-A homologue chicken lung lectin, while mannan binding lectin and collectins CL-L1 and CL-K1 only showed slightly changed expression during development. cSP-A protein could be detected as early as ED 18 in lung tissue using Western blotting, and expression increased steadily until day 28 post-hatch. Our observations are a first step towards understanding the role of this protein in vivo.
authors.affiliation: Department of Infectious Diseases and Immunology, Division of Molecular Host Defence, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands.
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Infectious Diseases and Immunology, Division of Molecular Host Defence, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands.
authors.forename: Tryntsje
authors.initials: T
authors.lastname: Cuperus
authors.affiliation: Department of Infectious Diseases and Immunology, Division of Molecular Host Defence, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands.
authors.forename: Albert
authors.initials: A
authors.lastname: van Dijk
authors.affiliation: Department of Cancer and Inflammation Research, Institute of Molecular Medicine, University of Southern Denmark, DK-5000, Odense, Denmark.
authors.forename: Karsten
authors.initials: K
authors.lastname: Skjødt
authors.affiliation: Department of Cancer and Inflammation Research, Institute of Molecular Medicine, University of Southern Denmark, DK-5000, Odense, Denmark.
authors.forename: Søren
authors.initials: S
authors.lastname: Hansen
authors.affiliation: Department of Infectious Diseases and Immunology, Division of Molecular Host Defence, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands.
authors.forename: Henk P
authors.initials: HP
authors.lastname: Haagsman
authors.affiliation: Department of Infectious Diseases and Immunology, Division of Molecular Host Defence, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands. Electronic address: E.J.A.Veldhuizen@uu.nl.
authors.forename: Edwin J A
authors.initials: EJ
authors.lastname: Veldhuizen
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D030161:Avian Proteins / Q000235:genetics / Q000378:metabolism*; D002645:Chickens / Q000276:immunology*; D037222:Collectins / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007113:Immunity, Innate; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008322:Mammals; D037601:Mannose-Binding Lectin / Q000378:metabolism; D010802:Phylogeny; D037662:Pulmonary Surfactant-Associated Protein A / Q000235:genetics / Q000378:metabolism*; D020545:Respiratory Mucosa / Q000378:metabolism*
chemical_list: D030161:Avian Proteins; C445459:COLEC10 protein, human; C518521:Colec11 protein, human; D037222:Collectins; D037601:Mannose-Binding Lectin; D037662:Pulmonary Surfactant-Associated Protein A
keywords: Chicken; Collectin; Innate immunity; Pulmonary surfactant protein