title: Separation of catechins and O-methylated (-)-epigallocatechin gallate using polyamide thin-layer chromatography.
abstract: Thin-layer chromatography (TLC) method for the separation and quantitative determination of seven related compounds: (+)-catechin (C), (-)-epicatechin (EC), (-)-epigallocatechin (EGC), (-)-epicatechin gallate (ECG), (-)-epigallocatechin gallate (EGCG), (-)-epigallocatechin-3-O-(3-O-methyl) gallate (EGCG3″Me) and (-)-epigallocatechin- 3-O-(4-O-methyl) gallate (EGCG4″Me) has been developed. The above-mentioned seven compounds have been resolved using polyamide TLC plates using a double-development with methanol followed by acetone/acetic acid (2:1, v/v). In addition, separation of the phenolic acids namely gallic acid, chlorogenic acid, and caffeic acid was achieved using the same solvent system. The applicability of the method was checked by screening of extracts of green, black, oolong, white tea and tea cultivars leaves.
authors.affiliation: National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients & Hunan Co-innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, PR China; Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, Hunan 410128, PR China.
authors.forename: Kunbo
authors.initials: K
authors.lastname: Wang
authors.affiliation: Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, Hunan 410128, PR China.
authors.forename: Qincao
authors.initials: Q
authors.lastname: Chen
authors.affiliation: National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients & Hunan Co-innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, PR China; Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, Hunan 410128, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, Hunan 410128, PR China.
authors.forename: Shuangshang
authors.initials: S
authors.lastname: Yu
authors.affiliation: National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients & Hunan Co-innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, PR China; Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, Hunan 410128, PR China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Lin
authors.affiliation: National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients & Hunan Co-innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, PR China; Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, Hunan 410128, PR China.
authors.forename: Jianan
authors.initials: J
authors.lastname: Huang
authors.affiliation: National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients & Hunan Co-innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, PR China; Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, Hunan 410128, PR China. Electronic address: zhonghualiu163@163.com.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Liu
mesh_terms: D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification*; D002855:Chromatography, Thin Layer / Q000379:methods*; D008745:Methylation; D009757:Nylons / Q000737:chemistry*
chemical_list: D009757:Nylons; D002392:Catechin; C045651:epigallocatechin gallate
keywords: Catechins; Double-development; O-methylated (−)-epigallocatechin gallate; Tea; Thin-layer chromatography