title: Sample-specific adaption of an improved electro-mechanical model of in vitro cardiac tissue.
abstract: We present an electromechanically coupled computational model for the investigation of a thin cardiac tissue construct consisting of human-induced pluripotent stem cell-derived atrial, ventricular and sinoatrial cardiomyocytes. The mechanical and electrophysiological parts of the finite element model, as well as their coupling are explained in detail. The model is implemented in the open source finite element code Code_Aster and is employed for the simulation of a thin circular membrane deflected by a monolayer of autonomously beating, circular, thin cardiac tissue. Two cardio-active drugs, S-Bay K8644 and veratridine, are applied in experiments and simulations and are investigated with respect to their chronotropic effects on the tissue. These results demonstrate the potential of coupled micro- and macroscopic electromechanical models of cardiac tissue to be adapted to experimental results at the cellular level. Further model improvements are discussed taking into account experimentally measurable quantities that can easily be extracted from the obtained experimental results. The goal is to estimate the potential to adapt the presented model to sample specific cell cultures.
authors.affiliation: Aachen University of Applied Sciences, Institute of Bioengineering, Biomechanics Lab, Heinrich-Mußmann-Straße 1, 52428 Jülich, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Frotscher
authors.affiliation: Aachen University of Applied Sciences, Institute of Bioengineering, Biomechanics Lab, Heinrich-Mußmann-Straße 1, 52428 Jülich, Germany.
authors.forename: Danita
authors.initials: D
authors.lastname: Muanghong
authors.affiliation: Aachen University of Applied Sciences, Institute of Bioengineering, Lab of Medical and Molecular Biology, Heinrich-Mußmann-Straße 1, 52428 Jülich, Germany.
authors.forename: Gözde
authors.initials: G
authors.lastname: Dursun
authors.affiliation: Axiogenesis AG, Nattermannallee 1, 50829 Cologne, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Goßmann
authors.affiliation: Aachen University of Applied Sciences, Institute of Bioengineering, Lab of Medical and Molecular Biology, Heinrich-Mußmann-Straße 1, 52428 Jülich, Germany.
authors.forename: Ayşegül
authors.initials: A
authors.lastname: Temiz-Artmann
authors.affiliation: Aachen University of Applied Sciences, Institute of Bioengineering, Biomechanics Lab, Heinrich-Mußmann-Straße 1, 52428 Jülich, Germany. Electronic address: m.staat@fh-aachen.de.
authors.forename: Manfred
authors.initials: M
authors.lastname: Staat
mesh_terms: D001696:Biomechanical Phenomena; D055724:Electrophysiological Phenomena; D020342:Finite Element Analysis; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology; D055595:Mechanical Phenomena; D008955:Models, Cardiovascular; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000502:physiology
keywords: Cardiac tissue; Computational biomechanics; Drug simulation; Electromechanical modeling; Frequency adaption; Hodgkin–Huxley models; Homogenization; hiPS cardiomyocytes