title: Role of annexin gene and its regulation during zebrafish caudal fin regeneration.
abstract: The molecular mechanism of epimorphic regeneration is elusive due to its complexity and limitation in mammals. Epigenetic regulatory mechanisms play a crucial role in development and regeneration. This investigation attempted to reveal the role of epigenetic regulatory mechanisms, such as histone H3 and H4 lysine acetylation and methylation during zebrafish caudal fin regeneration. It was intriguing to observe that H3K9,14 acetylation, H4K20 trimethylation, H3K4 trimethylation and H3K9 dimethylation along with their respective regulatory genes, such as GCN5, SETd8b, SETD7/9, and SUV39h1, were differentially regulated in the regenerating fin at various time points of post-amputation. Annexin genes have been associated with regeneration; this study reveals the significant up-regulation of ANXA2a and ANXA2b transcripts and their protein products during the regeneration process. Chromatin immunoprecipitation and PCR analysis of the regulatory regions of the ANXA2a and ANXA2b genes demonstrated the ability to repress two histone methylations, H3K27me3 and H4K20me3, in transcriptional regulation during regeneration. It is hypothesized that this novel insight into the diverse epigenetic mechanisms that play a critical role during the regeneration process may help to strategize the translational efforts, in addition to identifying the molecules involved in vertebrate regeneration.
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Saxena
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India.
authors.forename: Sruthi
authors.initials: S
authors.lastname: Purushothaman
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India.
authors.forename: Vuppalapaty
authors.initials: V
authors.lastname: Meghah
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India.
authors.forename: Bhawna
authors.initials: B
authors.lastname: Bhatti
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India.
authors.forename: Akhila
authors.initials: A
authors.lastname: Poruri
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India.
authors.forename: Mula G
authors.initials: MG
authors.lastname: Meena Lakshmi
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India.
authors.forename: Nukala
authors.initials: N
authors.lastname: Sarath Babu
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India.
authors.forename: Ch Lakshmi
authors.initials: ChL
authors.lastname: Narasimha Murthy
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India.
authors.forename: Komal K
authors.initials: KK
authors.lastname: Mandal
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India.
authors.forename: Arvind
authors.initials: A
authors.lastname: Kumar
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India.
authors.forename: Mohammed M
authors.initials: MM
authors.lastname: Idris
mesh_terms: D000671:Amputation, Surgical; D058500:Animal Fins / Q000293:injuries* / Q000502:physiology*; D000818:Animals; D017302:Annexins / Q000235:genetics* / Q000378:metabolism*; D015153:Blotting, Western; D004195:Disease Models, Animal; D044127:Epigenesis, Genetic; D018507:Gene Expression Regulation, Developmental; D006657:Histones / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D008745:Methylation; D011401:Promoter Regions, Genetic; D060888:Real-Time Polymerase Chain Reaction; D012038:Regeneration / Q000235:genetics*; D015027:Zebrafish / Q000235:genetics*
chemical_list: D017302:Annexins; D006657:Histones; D008239:Lysine
keywords: Annexin; histone methylation; histone modifications; regeneration; zebrafish