title: Discovery of a novel Parvovirinae virus, porcine parvovirus 7, by metagenomic sequencing of porcine rectal swabs.
abstract: Parvoviruses are a diverse group of viruses containing some of the smallest known species that are capable of infecting a wide range of animals. Metagenomic sequencing of pooled rectal swabs from adult pigs identified a 4103-bp contig consisting of two major open reading frames encoding proteins of 672 and 469 amino acids (aa) in length. BLASTP analysis of the 672-aa protein found 42.4 % identity to fruit bat (Eidolon helvum) parvovirus 2 (EhPV2) and 37.9 % to turkey parvovirus (TuPV) TP1-2012/HUN NS1 proteins. The 469-aa protein had no significant similarity to known proteins. Genetic and phylogenetic analyses suggest that PPV7, EhPV2, and TuPV represent a novel genus in the family Parvoviridae. Quantitative PCR screening of 182 porcine diagnostic samples found a total of 16 positives (8.6 %). Together, these data suggest that PPV7 is a highly divergent novel parvovirus prevalent within the US swine.
authors.affiliation: Kansas State Department of Diagnostic Medicine and Pathobiology, 1800 Denison Avenue, Manhattan, KS, 66506, USA.
authors.forename: Rachel M
authors.initials: RM
authors.lastname: Palinski
authors.affiliation: Kansas State Veterinary Diagnostic Laboratory, 1800 Denison Avenue, Manhattan, KS, 66506, USA.
authors.forename: Namita
authors.initials: N
authors.lastname: Mitra
authors.affiliation: Kansas State Department of Diagnostic Medicine and Pathobiology, 1800 Denison Avenue, Manhattan, KS, 66506, USA. bhause@vet.k-state.edu.|Kansas State Veterinary Diagnostic Laboratory, 1800 Denison Avenue, Manhattan, KS, 66506, USA. bhause@vet.k-state.edu.
authors.forename: Ben M
authors.initials: BM
authors.lastname: Hause
mesh_terms: D000818:Animals; D016679:Genome, Viral / Q000235:genetics*; D056186:Metagenomics / Q000379:methods; D010322:Parvoviridae Infections / Q000821:virology*; D028323:Parvovirus, Porcine / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000379:methods; D013552:Swine / Q000821:virology*; D013553:Swine Diseases / Q000821:virology*
keywords: Fecal; Metagenomic sequencing; Parvoviridae; Parvovirus; Swine