title: Establishment and characterization of fetal and maternal mesenchymal stem/stromal cell lines from the human term placenta.
abstract: BACKGROUND
  Human placental mesenchymal stem/stromal cells (MSC) are an attractive source of MSC with great therapeutic potential. However, primary MSC are difficult to study in vitro due to their limited lifespan and patient-to-patient variation.
  
  
  METHODS
  Fetal and maternal MSC were prepared from cells of the chorionic and basal plates of the placenta, respectively. Fetal and maternal MSC were transduced with the human telomerase reverse transcriptase (hTERT). Conventional stem cell assays assessed the MSC characteristics of the cell lines. Functional assays for cell proliferation, cell migration and ability to form colonies in soft agar were used to assess the whether transduced cells retained properties of primary MSC.
  
  
  RESULTS
  Fetal chorionic and maternal MSC were successfully transduced with hTERT to create the cell lines CMSC29 and DMSC23 respectively. The lifespans of CMSC29 and DMSC23 were extended in cell culture. Both cell lines retained important MSC characteristics including cell surface marker expression and multipotent differentiation potential. Neither of the cell lines was tumourigenic in vitro. Gene expression differences were observed between CMSC29 and DMSC23 cells and their corresponding parent, primary MSC. Both cell lines show similar migration potential to their corresponding primary, parent MSC.
  
  
  CONCLUSIONS
  The data show that transduced MSC retained important functional properties of the primary MSC. There were gene expression and functional differences between cell lines CMSC29 and DMSC23 that reflect their different tissue microenvironments of the parent, primary MSC. CMSC29 and DMSC23 cell lines could be useful tools for optimisation and functional studies of MSC.
authors.affiliation: University of Melbourne, Department of Obstetrics and Gynaecology, Royal Women's Hospital, Parkville, Victoria, 3052, Australia; Pregnancy Research Centre, Department of Maternal-Fetal Medicine, Royal Women's Hospital, Parkville, Victoria, 3052, Australia.
authors.forename: Sharon Q
authors.initials: SQ
authors.lastname: Qin
authors.affiliation: University of Melbourne, Department of Obstetrics and Gynaecology, Royal Women's Hospital, Parkville, Victoria, 3052, Australia; Pregnancy Research Centre, Department of Maternal-Fetal Medicine, Royal Women's Hospital, Parkville, Victoria, 3052, Australia.
authors.forename: Gina D
authors.initials: GD
authors.lastname: Kusuma
authors.affiliation: University of Melbourne, Department of Obstetrics and Gynaecology, Royal Women's Hospital, Parkville, Victoria, 3052, Australia; Pregnancy Research Centre, Department of Maternal-Fetal Medicine, Royal Women's Hospital, Parkville, Victoria, 3052, Australia.
authors.forename: Batla
authors.initials: B
authors.lastname: Al-Sowayan
authors.affiliation: University of Melbourne, Department of Obstetrics and Gynaecology, Royal Women's Hospital, Parkville, Victoria, 3052, Australia; Pregnancy Research Centre, Department of Maternal-Fetal Medicine, Royal Women's Hospital, Parkville, Victoria, 3052, Australia.
authors.forename: Rishika A
authors.initials: RA
authors.lastname: Pace
authors.affiliation: Mesenchymal Stem Cell Group, Division of Haematology, Institute of Medical and Veterinary Science/Hanson Institute/CSCR, University of Adelaide, Adelaide, SA, 5000, Australia.
authors.forename: Sandra
authors.initials: S
authors.lastname: Isenmann
authors.affiliation: Victorian Clinical Genetics Services (VCGS), Murdoch Children's Research Institute, Royal Children's Hospital, Parkville, Victoria, 3052, Australia; University of Melbourne, Department of Paediatrics, Royal Children's Hospital, Parkville, Victoria, 3052, Australia.
authors.forename: Mark D
authors.initials: MD
authors.lastname: Pertile
authors.affiliation: Mesenchymal Stem Cell Group, Division of Haematology, Institute of Medical and Veterinary Science/Hanson Institute/CSCR, University of Adelaide, Adelaide, SA, 5000, Australia.
authors.forename: Stan
authors.initials: S
authors.lastname: Gronthos
authors.affiliation: King Abdullah International Medical Research Center, College of Science and Health Professions, King Abdulaziz Medical City, National Guard Health Affairs, Mail Code 3124, P.O. Box 3660, Riyadh, 11481, Saudi Arabia; King Saud Bin Abdulaziz University for Health Sciences, College of Science and Health Professions, King Abdulaziz Medical City, National Guard Health Affairs, Mail Code 3124, P.O. Box 3660, Riyadh, 11481, Saudi Arabia.
authors.forename: Mohamed H
authors.initials: MH
authors.lastname: Abumaree
authors.affiliation: University of Melbourne, Department of Obstetrics and Gynaecology, Royal Women's Hospital, Parkville, Victoria, 3052, Australia; Pregnancy Research Centre, Department of Maternal-Fetal Medicine, Royal Women's Hospital, Parkville, Victoria, 3052, Australia.
authors.forename: Shaun P
authors.initials: SP
authors.lastname: Brennecke
authors.affiliation: University of Melbourne, Department of Obstetrics and Gynaecology, Royal Women's Hospital, Parkville, Victoria, 3052, Australia; Pregnancy Research Centre, Department of Maternal-Fetal Medicine, Royal Women's Hospital, Parkville, Victoria, 3052, Australia. Electronic address: bill.kalionis@thewomens.org.au.
authors.forename: Bill
authors.initials: B
authors.lastname: Kalionis
mesh_terms: D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D002823:Chorion / Q000166:cytology; D005260:Female; D005333:Fetus / Q000166:cytology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy; D061251:Primary Cell Culture / Q000379:methods*; D019098:Telomerase / Q000235:genetics; D047929:Term Birth; D014161:Transduction, Genetic
chemical_list: C509186:TERT protein, human; D019098:Telomerase
keywords: Fetal; Maternal; Mesenchymal stem cells; Mesenchymal stromal cells; Placenta; hTERT