title: Quantitative Susceptibility Mapping-Based Microscopy of Magnetic Resonance Venography (QSM-mMRV) for In Vivo Morphologically and Functionally Assessing Cerebromicrovasculature in Rat Stroke Model.
abstract: Abnormal cerebral oxygenation and vessel structure is a crucial feature of stroke. An imaging method with structural and functional information is necessary for diagnosis of stroke. This study applies QSM-mMRV (quantitative susceptibility mapping-based microscopic magnetic resonance venography) for noninvasively detecting small cerebral venous vessels in rat stroke model. First, susceptibility mapping is optimized and calculated from magnetic resonance (MR) phase images of a rat brain. Subsequently, QSM-mMRV is used to simultaneously provide information on microvascular architecture and venous oxygen saturation (SvO2), both of which can be used to evaluate the physiological and functional characteristics of microvascular changes for longitudinally monitoring and therapeutically evaluating a disease model. Morphologically, the quantification of vessel sizes using QSM-mMRV was 30% smaller than that of susceptibility-weighted imaging (SWI), which eliminated the overestimation of conventional SWI. Functionally, QSM-mMRV estimated an average SvO2 ranging from 73% to 85% for healthy rats. Finally, we also applied QSM to monitor the revascularization of post-stroke vessels from 3 to 10 days after reperfusion. QSM estimations of SvO2 were comparable to those calculated using the pulse oximeter standard metric. We conclude that QSM-mMRV is useful for longitudinally monitoring blood oxygen and might become clinically useful for assessing cerebrovascular diseases.
authors.affiliation: Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei 106, Taiwan.|Molecular Imaging Center, National Taiwan University, Taipei 106, Taiwan.|Department of Electrical Engineering, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Meng-Chi
authors.initials: MC
authors.lastname: Hsieh
authors.affiliation: Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 833, Taiwan.
authors.forename: Ching-Yi
authors.initials: CY
authors.lastname: Tsai
authors.affiliation: Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 833, Taiwan.
authors.forename: Min-Chiao
authors.initials: MC
authors.lastname: Liao
authors.affiliation: Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 833, Taiwan.
authors.forename: Jenq-Lin
authors.initials: JL
authors.lastname: Yang
authors.affiliation: Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 833, Taiwan.|Department of Biomedical Imaging and Radiological Sciences, National Yang Ming University, Taipei 112, Taiwan.
authors.forename: Chia-Hao
authors.initials: CH
authors.lastname: Su
authors.affiliation: Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei 106, Taiwan.|Molecular Imaging Center, National Taiwan University, Taipei 106, Taiwan.|Department of Electrical Engineering, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Jyh-Horng
authors.initials: JH
authors.lastname: Chen
mesh_terms: D000818:Animals; D002560:Cerebrovascular Circulation; D004195:Disease Models, Animal; D018810:Magnetic Resonance Angiography / Q000379:methods*; D008297:Male; D008833:Microcirculation; D010092:Oximetry / Q000379:methods; D010100:Oxygen / Q000008:administration & dosage* / Q000097:blood; D010690:Phlebography; D051381:Rats; D017207:Rats, Sprague-Dawley; D020521:Stroke / Q000097:blood / Q000000981:diagnostic imaging / Q000503:physiopathology
chemical_list: D010100:Oxygen