title: The Arabidopsis Protein CGLD11 Is Required for Chloroplast ATP Synthase Accumulation.
abstract: ATP synthases in chloroplasts (cpATPase) and mitochondria (mtATPase) are responsible for ATP production during photosynthesis and oxidative phosphorylation, respectively. Both enzymes consist of two multisubunit complexes, the membrane-bound coupling factor O and the soluble coupling factor 1. During cpATPase biosynthesis, several accessory factors facilitate subunit production and orchestrate complex assembly. Here, we describe a new auxiliary protein in Arabidopsis thaliana, which is required for cpATPase accumulation. AtCGLD11 (CONSERVED IN THE GREEN LINEAGE AND DIATOMS 11) is a protein without any known functional domain and shows dual localization to chloroplasts and mitochondria. Loss of AtCGLD11 function results in reduced levels of cpATPase and impaired photosynthetic performance with lower rates of ATP synthesis. In yeast two-hybrid experiments, AtCGLD11 interacts with the β subunits of the cpATPase and mtATPase. Our results suggest that AtCGLD11 functions in F1 assembly during cpATPase biogenesis, while its role in mtATPase biosynthesis may not, or not yet, be essential.
authors.affiliation: Lehrstuhl für Biochemie und Physiologie der Pflanzen (Botanik), Department Biologie I, Ludwig-Maximilians-Universität München, 82152 Planegg-Martinsried, Germany; Munich Centre for Integrated Protein Science CiPSM, Ludwig-Maximilians Universität München, Butenandtstr. 5 - 13, 81377 Munich, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Grahl
authors.affiliation: Lehrstuhl für Molekularbiologie der Pflanzen (Botanik), Department Biologie I, Ludwig-Maximilians-Universität München, 82152 Planegg-Martinsried, Germany.
authors.forename: Bennet
authors.initials: B
authors.lastname: Reiter
authors.affiliation: Lehrstuhl für Biochemie und Physiologie der Pflanzen (Botanik), Department Biologie I, Ludwig-Maximilians-Universität München, 82152 Planegg-Martinsried, Germany; Munich Centre for Integrated Protein Science CiPSM, Ludwig-Maximilians Universität München, Butenandtstr. 5 - 13, 81377 Munich, Germany.
authors.forename: Irene Luise
authors.initials: IL
authors.lastname: Gügel
authors.affiliation: Lehrstuhl für Molekularbiologie der Pflanzen (Botanik), Department Biologie I, Ludwig-Maximilians-Universität München, 82152 Planegg-Martinsried, Germany.
authors.forename: Evgenia
authors.initials: E
authors.lastname: Vamvaka
authors.affiliation: Lehrstuhl für Molekularbiologie der Pflanzen (Botanik), Department Biologie I, Ludwig-Maximilians-Universität München, 82152 Planegg-Martinsried, Germany.
authors.forename: Chiara
authors.initials: C
authors.lastname: Gandini
authors.affiliation: Plant Biochemistry, Heinrich-Heine-University Düsseldorf, Universitätsstrasse 1, 40225 Düsseldorf, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Jahns
authors.affiliation: Lehrstuhl für Biochemie und Physiologie der Pflanzen (Botanik), Department Biologie I, Ludwig-Maximilians-Universität München, 82152 Planegg-Martinsried, Germany; Munich Centre for Integrated Protein Science CiPSM, Ludwig-Maximilians Universität München, Butenandtstr. 5 - 13, 81377 Munich, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Soll
authors.affiliation: Lehrstuhl für Molekularbiologie der Pflanzen (Botanik), Department Biologie I, Ludwig-Maximilians-Universität München, 82152 Planegg-Martinsried, Germany. Electronic address: leister@lrz.uni-muenchen.de.
authors.forename: Dario
authors.initials: D
authors.lastname: Leister
authors.affiliation: Lehrstuhl für Molekularbiologie der Pflanzen (Botanik), Department Biologie I, Ludwig-Maximilians-Universität München, 82152 Planegg-Martinsried, Germany.
authors.forename: Thilo
authors.initials: T
authors.lastname: Rühle
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D025222:Chloroplast Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*; D002736:Chloroplasts / Q000201:enzymology / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D025222:Chloroplast Proton-Translocating ATPases
keywords: ATP synthase; assembly; chloroplast; dual targeting; mitochondria; photosynthesis