title: Rapid amplification of the RM-Yplex assay.
abstract: A multiplex PCR assay consisting of 13 Rapidly Mutating Y STR loci called RM-Yplex was previously developed. Platinum®Taq DNA polymerase was used to amplify the 13 Y STR loci in a single reaction at an amplification time of approximately 2.5 h. In order to shorten the process with reliable results, two DNA polymerases were tested with the multiplex. Phusion®Flash High Fidelity, TAKARA Z-taqTM, and Platinum®Taq DNA polymerases were investigated for conducting RM-Yplex assay at various PCR cycling conditions. Rapid, robust, and efficient amplification of all the markers within the multiplex were achieved. The amplification time was reduced from 2.5 h to less than 28 min with Phusion®Flash High Fidelity DNA polymerase using Veriti®PCR thermal cycler.
authors.affiliation: Directorate of Forensic Science Evidence, Public Prosecution, Manama, Bahrain. Aqeela.s@ppb.gov.bh, aqeela.gx@gmail.com.
authors.forename: Aqeela S
authors.initials: AS
authors.lastname: Abuidrees
authors.affiliation: Department of Forensic Sciences and Criminology, Dubai Police General Head Quarters, Dubai, United Arab Emirates.
authors.forename: Rashed H
authors.initials: RH
authors.lastname: Alghafri
authors.affiliation: School of Forensic and Investigative Sciences, University of Central Lancashire, Preston, UK.
authors.forename: Sibte
authors.initials: S
authors.lastname: Hadi
mesh_terms: D016172:DNA Fingerprinting / Q000379:methods*; D004586:Electrophoresis; D005260:Female; D053803:Forensic Genetics / Q000379:methods; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D009154:Mutation / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase
chemical_list: D019914:Taq Polymerase
keywords: DNA typing; Fast DNA polymerases; Forensic science; Rapid PCR; Rapidly mutating Y-short tandem repeats