title: The endosomal sorting complex required for transport (ESCRT) is required for the sensitivity of yeast cells to nickel ions in Saccharomyces cerevisiae.
abstract: Nickel is one of the toxic environment metal pollutants and is linked to various human diseases. In this study, through a functional genomics approach we have identified 16 nickel-sensitive and 22 nickel-tolerant diploid deletion mutants of budding yeast genes, many of which are novel players in the regulation of nickel homeostasis. The 16 nickel-sensitive mutants are of genes mainly involved in the protein folding, modification and destination and the cellular transport processes, while the 22 nickel-tolerant mutants are of genes encoding components of ESCRT complexes as well as protein factors involved in both the cell wall integrity maintenance and the vacuolar protein sorting process. In consistence with their phenotypes, most of these nickel-sensitive mutants show reduced intracellular nickel contents, while the majority of these nickel-tolerant mutants show elevated intracellular nickel contents, as compared to the wild type in response to nickel stress. Our data provides a basis for our understanding the regulation of nickel homeostasis and molecular mechanisms of nickel-induced human pathogenesis.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, No.1800, Lihu Road, Binhu District, Wuxi 214122, China The National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Luo
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, No.1800, Lihu Road, Binhu District, Wuxi 214122, China The National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Cao
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, No.1800, Lihu Road, Binhu District, Wuxi 214122, China The National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China linghuojiang@jiangnan.edu.cn.
authors.forename: Linghuo
authors.initials: L
authors.lastname: Jiang
mesh_terms: D004361:Drug Tolerance; D056827:Endosomal Sorting Complexes Required for Transport / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D005820:Genetic Testing; D007477:Ions / Q000378:metabolism / Q000633:toxicity*; D008826:Microbial Sensitivity Tests; D009532:Nickel / Q000378:metabolism / Q000633:toxicity*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*
chemical_list: D056827:Endosomal Sorting Complexes Required for Transport; D007477:Ions; D009532:Nickel
keywords: CWI; ESCRT; VPS; nickel; transition metal