title: Potential Diagnostic and Prognostic Value of Plasma Circulating MicroRNA-182 in Human Glioma.
abstract: BACKGROUND
  Previous studies showed the aberrant expression of microRNA-182 (miR-182) in glioma tissue. However, the exact role of circulating miR-182 in glioma remains unclear. Here, we confirmed the expression of plasma circulating miR-182 in glioma patients, and further explored its potential diagnostic and prognostic value.
  
  
  METHODS
  Real-time quantitative PCR (RT-PCR) was used to measure circulating cell-free miR-182 from 112 glioma patients and 54 healthy controls.
  
  
  RESULTS
  Our findings showed that the level of circulating miR-182 in glioma patients was higher than that in healthy controls (P<0.001), which was significantly associated with KPS score (P=0.025) and WHO grade (P<0.001). The area under the receiver operating characteristic (ROC) curve (AUC) was 0.778. The optimal cut-off value was 1.56, and the sensitivity and specificity were 58.5% and 85.2%, respectively. Interestingly, a high predictive value of circulating miR-182 was observed in high-grade glioma (AUC=0.815). However, the AUC was lower in low-grade glioma (AUC=0.621). Kaplan-Meier analysis demonstrated that the cumulative 5-year overall survival rate in the high miR-182 group was significantly lower than that in the low miR-182 group in both overall survival (OS) (P=0.003) and disease-free survival (DFS) (P=0.006). Moreover, multivariate Cox analysis revealed that circulating miR-182 was an independent prognostic indicator for OS (P=0.034) and DFS (P=0.013).
  
  
  CONCLUSIONS
  These results suggest that circulating miR-182 may be a potential noninvasive biomarker for the diagnosis and prognosis of human glioma.
authors.affiliation: Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong, China (mainland).
authors.forename: Yilei
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Department of Intensive Care Medicine, Liaocheng People's Hospital, Liaocheng, Shandong, China (mainland).
authors.forename: Lina
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Intensive Care Medicine, The People's Second Hospital of Liaocheng, Linqing, Shandong, China (mainland).
authors.forename: Zikun
authors.initials: Z
authors.lastname: Song
authors.affiliation: Department of Neurosurgery, Liaocheng People's Hospital, Liaocheng, Shandong, China (mainland).
authors.forename: Chuanjun
authors.initials: C
authors.lastname: Guo
authors.affiliation: Department of Neurosurgery, Liaocheng People's Hospital, Liaocheng, Shandong, China (mainland).
authors.forename: Jianxin
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Neurosurgery, Liaocheng People's Hospital, Liaocheng, Shandong, China (mainland).
authors.forename: Zhongmin
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong, China (mainland).
authors.forename: Shugan
authors.initials: S
authors.lastname: Zhu
mesh_terms: D001932:Brain Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000235:genetics*; D016022:Case-Control Studies; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000097:blood* / Q000175:diagnosis / Q000235:genetics*; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics*; D008875:Middle Aged; D015999:Multivariate Analysis; D011379:Prognosis; D012372:ROC Curve
chemical_list: D035683:MicroRNAs; C538984:Mirn182 microRNA, human