title: Spatial conservation studies of nucleobases in 10-23 DNAzyme by 2'-positioned isonucleotides and enantiomers for increased activity.
abstract: 2'-Positioned isonucleotides and enantiomers were used to evaluate the conservation of the spatial location of five adenines and two thymines in the catalytic core of 10-23 DNAzyme. The positive effect of isonucleotides at A15 and T8 along with inherent enzymatic resistance could be a tangible solution for the practical applications of 10-23 DNAzyme.
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, China. yangzj@bjmu.edu.cn.
authors.forename: Xiantao
authors.initials: X
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, China. yangzj@bjmu.edu.cn.
authors.forename: Zhangping
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: College of Life Sciences, Guizhou University, Guiyang 550025, China.
authors.forename: Junfei
authors.initials: J
authors.lastname: Zhu
authors.affiliation: College of Life Sciences, Guizhou University, Guiyang 550025, China.
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Li
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Taiping Road 27, Beijing 100850, China. hejunlin@bmi.ac.cn.
authors.forename: Junlin
authors.initials: J
authors.lastname: He
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, China. yangzj@bjmu.edu.cn.
authors.forename: Lihe
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, China. yangzj@bjmu.edu.cn.
authors.forename: Zhenjun
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000225:Adenine / Q000737:chemistry* / Q000378:metabolism; D055162:Biocatalysis; D020134:Catalytic Domain; D021881:DNA, Catalytic / Q000737:chemistry* / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000378:metabolism; D004789:Enzyme Activation; D056004:Molecular Dynamics Simulation; D013237:Stereoisomerism; D013941:Thymine / Q000737:chemistry* / Q000378:metabolism
chemical_list: D021881:DNA, Catalytic; D004277:DNA, Single-Stranded; C402582:RNA-cleaving DNA 10-23; D000225:Adenine; D013941:Thymine