title: A method for detailed analysis of the structure of mast cell secretory granules by negative contrast imaging.
abstract: Secretory granules (SGs) in mast cells contain various molecules that elicit allergy symptoms and are generally considered therapeutic targets. However, the biogenesis, maintenance, regulation, and recycling of these granules remain controversial, mainly due to the lack of suitable live-cell imaging methods. In this study, we applied negative contrast imaging with soluble green fluorescent protein (GFP) expressed in the cytoplasm as a method to validate structural information of mast cell SGs. We evaluated the accuracy of the method in detail, and we demonstrated that it can be used for quantitative analysis. Using this technique, secretory granules, the nucleus, mitochondria, and the cell body were visualized in individual RBL-2H3 mast cells without any influence. When combined with conventional multicolor fluorescence imaging, visualization of SG-associated proteins and SG-SG fusion was achieved. Moreover, 3D images were constructed based on this method, and detailed information on the number, size, and shape of individual SGs was obtained. We found that cell volume was correlated with SG number. In summary, the technique provides valuable and unique data, and will therefore advance SG research.
authors.affiliation: Department of Biochemistry, School of Medicine, Tokyo Women's Medical University, Kawada 8-1, Shinjuku, Tokyo 162-8666, Japan.
authors.forename: Shotaro
authors.initials: S
authors.lastname: Tanaka
authors.affiliation: Department of Biochemistry, School of Medicine, Tokyo Women's Medical University, Kawada 8-1, Shinjuku, Tokyo 162-8666, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Takakuwa
mesh_terms: D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus; D048429:Cell Size; D049452:Green Fluorescent Proteins / Q000378:metabolism; D021621:Imaging, Three-Dimensional; D008407:Mast Cells / Q000166:cytology* / Q000378:metabolism*; D061848:Optical Imaging / Q000379:methods*; D051381:Rats; D022142:Secretory Vesicles
chemical_list: D049452:Green Fluorescent Proteins