title: Determination of anti-tuberculosis drug concentration and distribution from sustained release microspheres in the vertebrae of a spinal tuberculosis rabbit model.
abstract: There have been several sustained-release materials containing anti-tuberculosis drug to enhance concentration in foci of spinal tuberculosis but what map by which composite drug delivery system is released is not known. Isoniazid (INH) lactic-co-glycolic acid (PLGA) and INH hydroxyapatite (HA) microspheres were prepared, respectively. The rabbits were modeled by tuberculosis strains and sustained-release microspheres were incubated in the foci. INH concentrations in the HA group were higher than those of INH and PLGA groups at L3, L4, and L5 vertebra 2 days after the drugs were embedded (p < 0.05). INH concentrations in the HA group were higher than those of INH and PLGA groups in L3 vertebra 4 days after the drugs were embedded (p < 0.05). INH concentrations in the PLGA group were higher than those of INH and HA groups in L5 vertebra; INH concentrations of the HA group were higher than those of INH and PLGA groups in L4 vertebra 6 days after the drugs were embedded (p < 0.05). These microspheres can enhance INH concentration in the foci. Furthermore, HA microspheres elicit osteogenic effects. HA is better than PLGA in terms of infiltration, but PLGA is better than HA in terms of distribution. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:200-208, 2017.
authors.affiliation: Department of Orthopedic Surgery, East Branch, Sichuan Provincial People's Hospital, Sichuan Academy of Medical Science, 585 North Da Mian Hong He Ave, Long Quan District, Chengdu, 610110, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.affiliation: Department of Spinal Surgery, Nanfang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, Guangzhou, 510515, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Orthopedic Surgery, East Branch, Sichuan Provincial People's Hospital, Sichuan Academy of Medical Science, 585 North Da Mian Hong He Ave, Long Quan District, Chengdu, 610110, People's Republic of China.
authors.forename: Shijun
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Spinal Surgery, Nanfang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, Guangzhou, 510515, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Department of Spinal Surgery, Nanfang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, Guangzhou, 510515, People's Republic of China.
authors.forename: Jianming
authors.initials: J
authors.lastname: Jiang
mesh_terms: D000818:Animals; D000995:Antitubercular Agents / Q000008:administration & dosage* / Q000032:analysis / Q000493:pharmacokinetics*; D004195:Disease Models, Animal; D016503:Drug Delivery Systems; D017886:Durapatite; D005260:Female; D007538:Isoniazid; D019344:Lactic Acid; D008159:Lumbar Vertebrae / Q000378:metabolism*; D008297:Male; D008863:Microspheres; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits; D014399:Tuberculosis, Spinal / Q000188:drug therapy*
chemical_list: D000995:Antitubercular Agents; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D017886:Durapatite; D007538:Isoniazid
keywords: anti-tuberculosis drugs; spinal tuberculosis; sustained-release