title: Comparison of the Effects of Plateletrich Plasma Prepared in Various Forms on the Healing of Dermal Wounds in Rats.
abstract: OBJECTIVE
  This study was designed to evaluate the effects of 3 different forms of platelet-rich plasma (PRP) on the healing process, wound healing rate, and histopathological changes that occur during recovery of open dermal wounds.
  
  
  METHODS
  A 2 cm x 1 cm full-thickness skin defect was made on the backs of 40 Wistar female rats that were divided into 4 groups of 10. In group 1 (control group), the wounds were cleaned with saline; in group 2, the wounds were covered with PRP gel obtained by single centrifugation; in group 3, the wounds were covered with liquid PRP obtained by double centrifugation; and in group 4, PRP gel obtained by double centrifugation with added thrombin was applied on the dermal wounds. All treatments were applied on postoperative days 1, 4, 7, and 10.
  
  
  RESULTS
  In all PRP groups, the wound closure was almost complete on day 14 while the wound contraction progressed more slowly in the control group. The mean histopathological scores of epithelialization, inflammation, and fibrosis were significantly better in all PRP groups than the scores in the control group.
  
  
  CONCLUSIONS
  In conclusion, although all PRP preparations had positive effects on dermal wound healing, double centrifuged PRP topical treatments (with or without thrombin activation) are more effective than single centrifuged PRP, and double centrifugation methods should be preferred for the preparation of PRP.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Esat Duymus
authors.forename: Selim
authors.initials: S
authors.lastname: Temel
authors.forename: Hilal
authors.initials: H
authors.lastname: Ozer
authors.forename: Mustafa
authors.initials: M
authors.lastname: Kemal Urhan
authors.forename: Ferudun
authors.initials: F
authors.lastname: Kaya
authors.forename: Fatih
authors.initials: F
authors.lastname: Aslan
authors.forename: Pinar
authors.initials: P
authors.lastname: Celepli
authors.forename: Mehmet
authors.initials: M
authors.lastname: Senes
authors.forename: Kubra
authors.initials: K
authors.lastname: Dogan
authors.forename: Serdar
authors.initials: S
authors.lastname: Kuru
authors.forename: Aziz
authors.initials: A
authors.lastname: Mutlu Barlas
authors.forename: Kemal
authors.initials: K
authors.lastname: Kismet
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005347:Fibroblasts / Q000473:pathology*; D018919:Neovascularization, Physiologic; D053657:Platelet-Rich Plasma; D051381:Rats; D017208:Rats, Wistar; D017695:Soft Tissue Injuries / Q000473:pathology*; D014945:Wound Healing