title: Molecular characterization of Sp110 gene in pigs.
abstract: Speckled 110 kDa (Sp110) plays an important role in infectious diseases, as revealed by studies in humans. However, little is known regarding porcine Sp110. To elucidate its potential role in porcine resistance to viral diseases, here, the complete coding sequence of porcine Sp110 gene and its 26 alternatively spliced isoforms were isolated using reverse transcription (RT)-polymerase chain reaction (PCR), and another seven splicing patterns were obtained using a minigene construct. Subcellular distribution of 11 representative isoforms was characterized in PK-15 cells transiently transfected with their respective GFP fusion constructs, and only isoforms (R and V) bearing all functional domains were localized in nucleus, indicating all the other isoforms lose normal functions of Sp110 owing to alternative splicing. Real-time quantitative PCR and competitive RT-PCR showed that both isoforms R and V had similar tissue expression profile, half-life and response to poly(I:C), a synthetic analog of viral double-stranded RNA, while the longer one (isoform R) was transcribed at a higher level. The results indicated that porcine Sp110 has a role in viral infection and that isoform R is the dominant active form. Overall the data provide potential resource for molecular breeding of pig resistant to diseases and contributes to breeding pigs resistant to viral infection.
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
authors.forename: Li-Zu
authors.initials: LZ
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
authors.forename: Qiu-Shi
authors.initials: QS
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Han
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
authors.forename: Jin-Kui
authors.initials: JK
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
authors.forename: Si-Yu
authors.initials: SY
authors.lastname: Shao
authors.affiliation: Institute of Animal Husbandry, Heilongjiang Academy of Agricultural Sciences, Harbin, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Institute of Animal Husbandry, Heilongjiang Academy of Agricultural Sciences, Harbin, China. liudi1963@163.com.
authors.forename: Di
authors.identifier: http://orcid.org/0000-0002-6618-6675
authors.initials: D
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, China. xiuqin163@163.com.
authors.forename: Xiu-Qin
authors.initials: XQ
authors.lastname: Yang
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods*; D005786:Gene Expression Regulation / Q000187:drug effects; D009687:Nuclear Proteins / Q000235:genetics*; D011070:Poly I-C; D011119:Polynucleotides / Q000494:pharmacology; D013552:Swine; D013556:Swine, Miniature / Q000235:genetics*; D014018:Tissue Distribution
chemical_list: D009687:Nuclear Proteins; D011119:Polynucleotides; D011070:Poly I-C
keywords: Alternative splicing; Expression; Pig; Sp110; Subcellular localization