title: 1,8-naphthalimide modified [12]aneN₃ compounds as selective and sensitive probes for Cu²⁺ ions and ATP in aqueous solution and living cells.
abstract: A new fluorescent probe 1 featuring one 1,8-naphthalimide and two [12]aneN3 units was synthesized. In the presence of Cu(2+) ions, the fluorescence emission of 1 was quenched by a factor of 127-fold and no interference by other metal ions was observed under physiological conditions. By means of titration and a Job's plot it was established that 1 forms a complex with Cu(2+) ions in a 1:2 ratio. The fluorescence of the 1-Cu(2+) complex was recovered by the addition of Adenosine-5'-triphosphate (ATP) in aqueous solution. Due to its low cytotoxicity, good water solubility, and high sensitivity, probe 1 was successfully applied in the sequential recognition of Cu(2+) and ATP in aqueous solution and HeLa cells. The highly selective and sensitive ability of 1-Cu(2+) complex to detect ATP even enables its bio-analytical applications in real-time imaging in living cells.
authors.affiliation: Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University, Xinjiekouwai Street 19, Beijing 100875, China.
authors.forename: Yong-Guang
authors.initials: YG
authors.lastname: Gao
authors.affiliation: Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University, Xinjiekouwai Street 19, Beijing 100875, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University, Xinjiekouwai Street 19, Beijing 100875, China.
authors.forename: You-Di
authors.initials: YD
authors.lastname: Shi
authors.affiliation: Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University, Xinjiekouwai Street 19, Beijing 100875, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University, Xinjiekouwai Street 19, Beijing 100875, China. Electronic address: luzl@bnu.edu.cn.
authors.forename: Zhong-Lin
authors.initials: ZL
authors.lastname: Lu
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis* / Q000737:chemistry*; D002470:Cell Survival; D003300:Copper / Q000032:analysis* / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D057230:Limit of Detection; D057054:Molecular Imaging; D053644:Naphthalimides / Q000138:chemical synthesis / Q000737:chemistry*; D012996:Solutions; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D053644:Naphthalimides; D012996:Solutions; D014867:Water; D003300:Copper; D000255:Adenosine Triphosphate
keywords: ATP; Cu(2+); Naphthalimide; Phosphates; [12]aneN(3)