title: A possible connection between tumor necrosis factor alpha and adropin levels in polycystic ovary syndrome.
abstract: BACKGROUND
  Adropin is a peptide hormone implicated in the regulation of insulin sensitivity and energy homeostasis. Polycystic ovary syndrome (PCOS) is a metabolic and reproductive disease associated with insulin resistance. It has been demonstrated that various inflammatory markers increased in PCOS including TNF-α. TNF-α regulates the secretion of certain peptides which play a crucial role in glucose and lipid homeostasis. There is also some evidence of a link between TNF-α and adropin.
  
  
  OBJECTIVE
  To ascertain whether there is an association between circulating adropin levels and TNF-α in PCOS.
  
  
  METHODS
  152 women with PCOS and 152 age- and body mass index-matched controls without PCOS were recruited for this cross-sectional study.
  
  
  METHODS
  Adropin and TNF-α levels were measured using ELISA.
  
  
  RESULTS
  Adropin levels were lower in the PCOS group compared with the control group (7.43 ± 0.79 vs. 9.42 ± 0.76 ng/ml, P < 0.001), whereas TNF-α levels were higher (49.93 ± 3.39 vs. 35.83 ± 2.47 pg/ml, P < 0.001). A strongly negative correlation was found between circulating adropin levels and TNF-α levels in women with PCOS (r = -0.407, P < 0.001). Binary logistic regression analysis revealed that decreased adropin levels were significantly associated with high odds of having PCOS, although, after adjustment for TNF-α, this link vanished. Additionally, multiple linear regression analysis showed that HOMA-IR and TFN-α independently predicted adropin levels.
  
  
  CONCLUSIONS
  Serum adropin levels are significantly decreased in PCOS and are inversely associated with TNF-α. Further dissection of the nature of this association can open new therapeutic options for metabolic diseases.
authors.affiliation: Department of Biochemistry and Clinical Biochemistry, Dokuz Eylul University Faculty of Medicine, 35340, Inciralti, Izmir, Turkey.
authors.forename: T
authors.initials: T
authors.lastname: Kume
authors.affiliation: Division of Endocrinology and Metabolism (PCOS Research Group), Department of Internal Medicine, Dokuz Eylul University Faculty of Medicine, 35340, Inciralti, Izmir, Turkey. drmehmetcalan@gmail.com.
authors.forename: M
authors.initials: M
authors.lastname: Calan
authors.affiliation: Department of Obstetrics and Gynecology, Manisa Merkezefendi State Hospital, 45020, Merkezefendi, Manisa, Turkey.
authors.forename: O
authors.initials: O
authors.lastname: Yilmaz
authors.affiliation: Division of Endocrinology and Metabolism, Department of Internal Medicine (PCOS Research Group), Izmir Bozyaka Training and Research Hospital, 35170, Bozyaka, Izmir, Turkey.
authors.forename: G U
authors.initials: GU
authors.lastname: Kocabas
authors.affiliation: Department of Bioengineering, Ege University, 35100, Bornova, Izmir, Turkey.
authors.forename: P
authors.initials: P
authors.lastname: Yesil
authors.affiliation: Department of Obstetrics and Gynecology, Manisa Merkezefendi State Hospital, 45020, Merkezefendi, Manisa, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Temur
authors.affiliation: Department of Obstetrics and Gynecology, Medical Park Hospital, Izmir University Faculty of Medicine, 35575, Izmir, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Bicer
authors.affiliation: Department of Biochemistry and Clinical Biochemistry, Dokuz Eylul University Faculty of Medicine, 35340, Inciralti, Izmir, Turkey.
authors.forename: O G
authors.initials: OG
authors.lastname: Calan
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood*; D001798:Blood Proteins; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007333:Insulin Resistance; D036341:Intercellular Signaling Peptides and Proteins; D009765:Obesity; D010455:Peptides / Q000097:blood*; D011085:Polycystic Ovary Syndrome / Q000097:blood* / Q000473:pathology*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; C568440:Enho protein, human; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D014409:Tumor Necrosis Factor-alpha
keywords: Adropin; Cytokine; Insulin resistance; Polycystic ovary syndrome; Tumor necrosis factor-α