title: Bioactive lignan constituents from the twigs of Sambucus williamsii.
abstract: As part of our ongoing search for bioactive constituents of natural Korean medicinal plants, three new lignan derivatives, sambucasinol A-C (1-3), together with 7 known compounds (4-10) were isolated from the twigs of Sambucus williamsii. The structures of these new compounds were determined by a combination of 1D and 2D NMR spectroscopic data analysis, as well as circular dichroism (CD) spectroscopy studies. Here, we evaluated the anti-inflammatory effects of 1-10 in lipopolysaccharide (LPS)-stimulated murine microglia BV-2 cells. Compounds 1-3 exhibited significant inhibitory effects on nitric oxide production in LPS-activated BV-2 cells, with IC50 values of 6.82, 7.04, and 14.70 μM, respectively. Additionally, we evaluated the effects of compounds 1-10 on NGF induction in a C6 rat glioma cell line. Compounds 1-3 upregulated NGF secretion to 183.95 ± 2.63%, 153.99 ± 5.15%, and 155.96 ± 5.15%, respectively, without any significant cell toxicity. Moreover, all isolates were evaluated for their cytotoxicity against A549, SK-OV-3, SK-MEL-2, and XF498 cell lines. Compounds 1-3 showed consistent cytotoxicity against the four human cell lines, with IC50 values in the range of 11.07-19.62 μM.
authors.affiliation: Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University, 300 Chonchon-dong, Jangan-ku, Suwon 16419, Republic of Korea.
authors.forename: Won Se
authors.initials: WS
authors.lastname: Suh
authors.affiliation: Gachon Institute of Pharmaceutical Science, Gachon University, 191 Hambakmoero, Yeonsu-gu, Incheon 21936, Republic of Korea; College of Pharmacy, Gachon University, 191 Hambakmoero, Yeonsu-gu, Incheon 21936, Republic of Korea.
authors.forename: Lalita
authors.initials: L
authors.lastname: Subedi
authors.affiliation: Gachon Institute of Pharmaceutical Science, Gachon University, 191 Hambakmoero, Yeonsu-gu, Incheon 21936, Republic of Korea; College of Pharmacy, Gachon University, 191 Hambakmoero, Yeonsu-gu, Incheon 21936, Republic of Korea.
authors.forename: Sun Yeou
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.
authors.forename: Sang Un
authors.initials: SU
authors.lastname: Choi
authors.affiliation: Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University, 300 Chonchon-dong, Jangan-ku, Suwon 16419, Republic of Korea. Electronic address: krlee@skku.edu.
authors.forename: Kang Ro
authors.initials: KR
authors.lastname: Lee
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000494:pharmacology; D051379:Mice; D008968:Molecular Conformation; D009414:Nerve Growth Factors / Q000378:metabolism; D018547:Plant Stems / Q000737:chemistry; D051381:Rats; D027923:Sambucus / Q000737:chemistry*; D013329:Structure-Activity Relationship
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000972:Antineoplastic Agents, Phytogenic; D017705:Lignans; D008070:Lipopolysaccharides; D009414:Nerve Growth Factors
keywords: Anti-inflammation; Cytotoxicity; Lignan; NGF regulation; Sambucus williamsii