title: Olfactory perception and behavioral effects of sex pheromone gland components in Helicoverpa armigera and Helicoverpa assulta.
abstract: Two sympatric species Helicoverpa armigera and Helicoverpa assulta use (Z)-11-hexadecenal and (Z)-9-hexadecenal as sex pheromone components in reverse ratio. They also share several other pheromone gland components (PGCs). We present a comparative study on the olfactory coding mechanism and behavioral effects of these additional PGCs in pheromone communication of the two species using single sensillum recording, in situ hybridization, calcium imaging, and wind tunnel. We classify antennal sensilla types A, B and C into A, B1, B2, C1, C2 and C3 based on the response profiles, and identify the glomeruli responsible for antagonist detection in both species. The abundance of these sensilla types when compared with the number of OSNs expressing each of six pheromone receptors suggests that HarmOR13 and HassOR13 are expressed in OSNs housed within A type sensilla, HarmOR14b within B and C type sensilla, while HassOR6 and HassOR16 within some of C type sensilla. We find that for H. armigera, (Z)-11-hexadecenol and (Z)-11-hexadecenyl acetate act as behavioral antagonists. For H. assulta, instead, (Z)-11-hexadecenyl acetate acts as an agonist, while (Z)-9-hexadecenol, (Z)-11-hexadecenol and (Z)-9-hexadecenyl acetate are antagonists. The results provide an overall picture of intra- and interspecific olfactory and behavioral responses to all PGCs in two sister species.
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China.
authors.forename: Meng
authors.initials: M
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Hou
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China.
authors.forename: Han
authors.initials: H
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China.
authors.forename: Ling-Qiao
authors.initials: LQ
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China.
authors.forename: Chen-Zhu
authors.initials: CZ
authors.lastname: Wang
mesh_terms: D000447:Aldehydes / Q000378:metabolism*; D000818:Animals; D057506:Arthropod Antennae / Q000294:innervation / Q000378:metabolism / Q000502:physiology; D055724:Electrophysiological Phenomena; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D017403:In Situ Hybridization; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D009036:Moths / Q000737:chemistry / Q000145:classification / Q000502:physiology*; D055696:Olfactory Perception / Q000502:physiology*; D018034:Olfactory Receptor Neurons / Q000502:physiology; D043563:Receptors, Pheromone / Q000235:genetics / Q000378:metabolism; D012543:Scent Glands / Q000737:chemistry / Q000502:physiology*; D058618:Sensilla / Q000294:innervation / Q000378:metabolism / Q000502:physiology; D012724:Sex Attractants / Q000378:metabolism*; D013045:Species Specificity
chemical_list: C587835:9-hexadecenal; D000447:Aldehydes; D019476:Insect Proteins; D043563:Receptors, Pheromone; D012724:Sex Attractants; C061128:11-hexadecenal