title: Phylogenetic characterization of the first Ungulate tetraparvovirus 2 detected in pigs in Brazil.
abstract: Ungulate tetraparvovirus 2 (UTV2), formerly known as porcine hokovirus due to its discovery in Hong Kong, is closely related to a Primate tetraparvovirus (human PARV-4) and Ungulate tetraparvovirus 1 (bovine hokovirus). Until now, UTV2 was detected in European, Asian and North American countries, but its occurrence in Latin America is still unknown. This study describes the first report of UTV2 in Brazil, as well as its phylogenetic characterization. Tissue samples (lymph node, lung, liver, spleen and kidney) of 240 piglets from eight different herds (30 animals each herd) were processed for DNA extraction. UTV2 DNA was detected by PCR and the entire VP1/VP2 gene was sequenced for phylogenetic analysis. All pigs from this study displayed postweaning multisystemic wasting syndrome (PMWS). UTV2 was detected in 55.3% of the samples distributed in the variety of porcine tissues investigated, as well as detected in almost all herds, with one exception. The phylogenetic analysis demonstrated that Brazilian UTV2 sequences were more closely related to sequences from Europe and United States.
authors.affiliation: Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Brazil.
authors.forename: Carine Kunzler
authors.initials: CK
authors.lastname: Souza
authors.affiliation: Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Brazil.
authors.forename: André Felipe
authors.initials: AF
authors.lastname: Streck
authors.affiliation: Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Brazil.
authors.forename: Karla Ratje
authors.initials: KR
authors.lastname: Gonçalves
authors.affiliation: Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Brazil.
authors.forename: Luciane Dubina
authors.initials: LD
authors.lastname: Pinto
authors.affiliation: Laboratório de Imunologia e Biologia Molecular, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Brazil.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Ravazzolo
authors.affiliation: Setor de Suínos, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Brazil.
authors.forename: David Emílio Dos Santos Neves
authors.initials: DE
authors.lastname: de Barcellos
authors.affiliation: Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Brazil. Electronic address: claudio.canal@ufrgs.br.
authors.forename: Cláudio Wageck
authors.initials: CW
authors.lastname: Canal
mesh_terms: D000818:Animals; D001938:Brazil; D004279:DNA, Viral / Q000235:genetics; D010322:Parvoviridae Infections / Q000662:veterinary* / Q000821:virology; D019219:Parvovirinae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D013552:Swine; D013553:Swine Diseases / Q000821:virology*
chemical_list: D004279:DNA, Viral
keywords: Detection; Hokovirus; Phylogeny; Porcine; Tetraparvovirus; Ungulate