title: Reactive Microcontact Printing of DNA Probes on (DMA-NAS-MAPS) Copolymer-Coated Substrates for Efficient Hybridization Platforms.
abstract: High-performing hybridization platforms fabricated by reactive microcontact printing of DNA probes are presented. Multishaped PDMS molds are used to covalently bind oligonucleotides over a functional copolymer (DMA-NAS-MAPS) surface. Printed structures with minimum width of about 1.5 μm, spaced by 10 μm, are demonstrated, with edge corrugation lower than 300 nm. The quantification of the immobilized surface probes via fluorescence imaging gives a remarkable concentration of 3.3 × 10(3) oligonucleotides/μm(2), almost totally active when used as probes in DNA-DNA hybridization assays. Indeed, fluorescence and atomic force microscopy show a 95% efficiency in target binding and uniform DNA hybridization over printed areas.
authors.affiliation: Dipartimento di Elettronica Informazione e Bioingegneria, Politecnico di Milano , Via G. Colombo 81, 20133, Milano, Italy.
authors.forename: Rossella
authors.initials: R
authors.lastname: Castagna
authors.affiliation: Institut de Science et d'Ingénierie Supramoléculaires (ISIS), Université de Strasbourg , 8 allée Gaspard Monge, 67000, Strasbourg, France.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Bertucci
authors.affiliation: Institut de Science et d'Ingénierie Supramoléculaires (ISIS), Université de Strasbourg , 8 allée Gaspard Monge, 67000, Strasbourg, France.
authors.forename: Eko Adi
authors.initials: EA
authors.lastname: Prasetyanto
authors.affiliation: Dipartimento di Fisica, Politecnico di Milano , Via G. Colombo 81, 20133, Milano, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Monticelli
authors.affiliation: Dipartimento di Fisica, Politecnico di Milano , Via G. Colombo 81, 20133, Milano, Italy.
authors.forename: Dario Valter
authors.initials: DV
authors.lastname: Conca
authors.affiliation: Dipartimento di Fisica, Politecnico di Milano , Via G. Colombo 81, 20133, Milano, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Massetti
authors.affiliation: Dipartimento di Fisica, Politecnico di Milano , Via G. Colombo 81, 20133, Milano, Italy.
authors.forename: Parikshit Pratim
authors.initials: PP
authors.lastname: Sharma
authors.affiliation: Istituto di Chimica del Riconoscimento Molecolare, Consiglio Nazionale delle Ricerche , Via Mario Bianco 9, 20131, Milano, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Damin
authors.affiliation: Istituto di Chimica del Riconoscimento Molecolare, Consiglio Nazionale delle Ricerche , Via Mario Bianco 9, 20131, Milano, Italy.
authors.forename: Marcella
authors.initials: M
authors.lastname: Chiari
authors.affiliation: Institut de Science et d'Ingénierie Supramoléculaires (ISIS), Université de Strasbourg , 8 allée Gaspard Monge, 67000, Strasbourg, France.
authors.forename: Luisa
authors.initials: L
authors.lastname: De Cola
authors.affiliation: Dipartimento di Fisica, Politecnico di Milano , Via G. Colombo 81, 20133, Milano, Italy.|IFN-CNR Via Colombo 81, 20133 Milano, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Bertacco
mesh_terms: D002232:Carbocyanines / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D013388:Succinimides / Q000737:chemistry*
chemical_list: D002232:Carbocyanines; D015342:DNA Probes; D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D008689:Methacrylates; C000613817:N,N-dimethylacrylamide-N-acryloyloxysuccinimide-3-(trimethoxysilyl)propyl methacrylate; D009838:Oligodeoxyribonucleotides; D013388:Succinimides; C089187:cyanine dye 3