title: Collagen-Hydroxyapatite Scaffolds Induce Human Adipose Derived Stem Cells Osteogenic Differentiation In Vitro.
abstract: Mesenchymal stem cells (MSCs) play a crucial role in regulating normal skeletal homeostasis and, in case of injury, in bone healing and reestablishment of skeletal integrity. Recent scientific literature is focused on the development of bone regeneration models where MSCs are combined with biomimetic three-dimensional scaffolds able to direct MSC osteogenesis. In this work the osteogenic potential of human MSCs isolated from adipose tissue (hADSCs) has been evaluated in vitro in combination with collagen/Mg doped hydroxyapatite scaffolds. Results demonstrate the high osteogenic potential of hADSCs when cultured in specific differentiation induction medium, as revealed by the Alizarin Red S staining and gene expression profile analysis. In combination with collagen/hydroxyapatite scaffold, hADSCs differentiate into mature osteoblasts even in the absence of specific inducing factors; nevertheless, the supplement of the factors markedly accelerates the osteogenic process, as confirmed by the expression of specific markers of pre-osteoblast and mature osteoblast stages, such as osterix, osteopontin (also known as bone sialoprotein I), osteocalcin and specific markers of extracellular matrix maturation and mineralization stages, such as ALPL and osteonectin. Hence, the present work demonstrates that the scaffold per se is able to induce hADSCs differentiation, while the addition of osteo-inductive factors produces a significant acceleration of the osteogenic process. This observation makes the use of our model potentially interesting in the field of regenerative medicine for the treatment of bone defects.
authors.affiliation: IOM Ricerca, Viagrande, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Calabrese
authors.affiliation: IOM Ricerca, Viagrande, Italy.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Giuffrida
authors.affiliation: Finceramica, Faenza, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Fabbi
authors.affiliation: Finceramica, Faenza, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Figallo
authors.affiliation: Department of Biomedical and Biotechnological Sciences, Physiology Section, University of Catania, Catania, Italy.
authors.forename: Debora
authors.initials: D
authors.lastname: Lo Furno
authors.affiliation: IOM Ricerca, Viagrande, Italy.|Department of Biomedical and Biotechnological Sciences, Physiology Section, University of Catania, Catania, Italy.
authors.forename: Rosario
authors.initials: R
authors.lastname: Gulino
authors.affiliation: Department of Experimental Oncology, Mediterranean Institute of Oncology, Viagrande, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Colarossi
authors.affiliation: Department of Experimental Oncology, Mediterranean Institute of Oncology, Viagrande, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Fullone
authors.affiliation: Department of Biomedical and Biotechnological Sciences, Physiology Section, University of Catania, Catania, Italy.
authors.forename: Rosario
authors.initials: R
authors.lastname: Giuffrida
authors.affiliation: Department of Biomedical and Biotechnological Sciences, Physiology Section, University of Catania, Catania, Italy.
authors.forename: Rosalba
authors.initials: R
authors.lastname: Parenti
authors.affiliation: IOM Ricerca, Viagrande, Italy.|Department of Experimental Oncology, Mediterranean Institute of Oncology, Viagrande, Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Memeo
authors.affiliation: IOM Ricerca, Viagrande, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Forte
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D001672:Biocompatible Materials; D032701:Biomimetics; D001842:Bone and Bones / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D003094:Collagen / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D020869:Gene Expression Profiling; D006801:Humans; D066298:In Vitro Techniques; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D003094:Collagen; D017886:Durapatite