title: Pharmacokinetics and metabolism study of veratramine in mice after oral administration using LC-MS/MS.
abstract: A simple and sensitive high-performance liquid chromatography coupled with hybrid triple quadrupole-linear ion trap mass spectrometry (Q-trap-MS) method was developed and validated for the determination of veratramine, the major bioactive and neurotoxic component in Veratrum nigrum L. Veratramine and the internal standard (IS) were separated with a Waters Symmetry C18 column and eluted with a gradient mobile phase system containing acetonitrile and 0.1% aqueous formic acid. The analysis was performed by using positive electrospray ionization mode with multiple reaction monitoring (MRM). Transition ions of m/z 410.2 → 295.2 for veratramine and m/z 426.1 → 113.8 for the IS were monitored. The method was validated with a good linearity in the range of 1-1000 ng/mL and lower limit of quantification of 1 ng/mL. The precision (CV) of intra- and inter-day ranged from 3.92 to 7.29%, while the accuracy (bias) intra- and inter-day were between -4.78 and 1.65%. The recovery, stability and matrix effect were within the acceptable ranges. Five metabolites of veratramine, including four hydroxylated and one sulfated metabolites, were tentatively identified using predictive MRM-information dependent acquisition-enhanced product ion mode (predictive MRM-IDA-EPI). The developed method was successfully applied to the pharmacokinetic and metabolic study of veratramine in mice after oral administration of veratramine. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Institute of Pharmacy, Pharmaceutical College, Henan University, Kaifeng, 475004, People's Republic of China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Cong
authors.affiliation: State Key Laboratory of Cotton Biology, College of Life Sciences, Henan University, Kaifeng, 475004, People's Republic of China.
authors.forename: Jun-Li
authors.initials: JL
authors.lastname: Zhang
authors.affiliation: Institute of Pharmacy, Pharmaceutical College, Henan University, Kaifeng, 475004, People's Republic of China.
authors.forename: Sha-Sha
authors.initials: SS
authors.lastname: Li
authors.affiliation: Institute of Pharmacy, Pharmaceutical College, Henan University, Kaifeng, 475004, People's Republic of China.
authors.forename: Shan
authors.initials: S
authors.lastname: Shen
authors.affiliation: Institute of Pharmacy, Pharmaceutical College, Henan University, Kaifeng, 475004, People's Republic of China.
authors.forename: Jiang-Ying
authors.initials: JY
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Hong Kong Baptist University, Hong Kong SAR, People's Republic of China.
authors.forename: Zongwei
authors.initials: Z
authors.lastname: Cai
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D057230:Limit of Detection; D051379:Mice; D012015:Reference Standards; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014704:Veratrum Alkaloids / Q000008:administration & dosage / Q000493:pharmacokinetics*
chemical_list: D014704:Veratrum Alkaloids; C009649:veratramine
keywords: Veratrum nigrum; metabolism; mouse; pharmacokinetics; veratramine