title: Actin3 promoter reveals undulating F-actin bundles at shanks and dynamic F-actin meshworks at tips of tip-growing pollen tubes.
abstract: The dynamic actin cytoskeleton of pollen tubes is both the driver of the tip growth and the organizer of cell polarity. In order to understand this fast re-arranging cytoskeletal system, we need reliable constructs expressed under relevant promoters. Here we are reporting that the Lifeact reporter, expressed under the pollen-specific Actin3 promoter, visualizes very dynamic F-actin elements both in germinating pollen grains and tip-growing pollen tubes. Importantly, we have documented very active actin polymerization at the cell periphery, especially in the bulging area during pollen germination and in the apical clear zone. Expression of the Lifeact reporter under control of the pollen-specific Actin3 promoter revealed 2 new aspects: (i) long F-actin bundles in pollen tube shanks are dynamic, showing undulating movements, (ii) subapical 'actin collars' or 'fringes' are absent.
authors.affiliation: a Comenius University Science Park, Comenius University , Bratislava , Slovakia.|b Institute of Botany, Slovak Academy of Sciences , Bratislava , Slovakia.
authors.forename: Ján
authors.initials: J
authors.lastname: Jásik
authors.affiliation: a Comenius University Science Park, Comenius University , Bratislava , Slovakia.|c Department of Botany , Faculty of Natural Science, Comenius University , Bratislava , Slovakia.
authors.forename: Karol
authors.initials: K
authors.lastname: Mičieta
authors.affiliation: d Department of Plant Cell Biology , IZMB, University of Bonn , Bonn , Germany.
authors.forename: Wei
authors.initials: W
authors.lastname: Siao
authors.affiliation: c Department of Botany , Faculty of Natural Science, Comenius University , Bratislava , Slovakia.
authors.forename: Boris
authors.initials: B
authors.lastname: Voigt
authors.affiliation: a Comenius University Science Park, Comenius University , Bratislava , Slovakia.|e Department of Molecular Biology , Faculty of Natural Sciences , Mlynská dolina , Slovakia.
authors.forename: Stanislav
authors.initials: S
authors.lastname: Stuchlík
authors.affiliation: f Max Planck Institute for Plant Breeding Research , Köln , Germany.
authors.forename: Elmon
authors.initials: E
authors.lastname: Schmelzer
authors.affiliation: a Comenius University Science Park, Comenius University , Bratislava , Slovakia.|e Department of Molecular Biology , Faculty of Natural Sciences , Mlynská dolina , Slovakia.
authors.forename: Ján
authors.initials: J
authors.lastname: Turňa
authors.affiliation: b Institute of Botany, Slovak Academy of Sciences , Bratislava , Slovakia.|d Department of Plant Cell Biology , IZMB, University of Bonn , Bonn , Germany.
authors.forename: František
authors.initials: F
authors.lastname: Baluška
mesh_terms: D000199:Actins / Q000032:analysis / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D016764:Cell Polarity; D018525:Germination; D053205:Pollen Tube / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D058105:Polymerization; D011401:Promoter Regions, Genetic
chemical_list: D000199:Actins
keywords: Actin cytoskeleton; actin3 promoter; lifeact construct; polarity; pollen tubes