title: Regulating effect of activated NF-κB on edema induced by traumatic brain injury of rats.
abstract: OBJECTIVE
  To observe the effect of nuclear transcription factor-κB (NF-κB) on cerebral edema in rats with traumatic brain injury (TBI).
  
  
  METHODS
  Male SD rats with fluid percussion injury (FPI) were selected. After separation and culture, rats' astrocytes all suffered FPI. The expression of NF-κB and the water content were detected at the animal and cellular levels, while the activity of NOX was evaluated at the cellular level.
  
  
  RESULTS
  According to the results, the positive expression of NF-κB and expression of mRNA were significantly increased and the water content was increased for rats after TBI, while NF-κB inhibitor BAY11-7082 could significantly reduce the effect of TBI. 1 and 3 h after FPI of astrocytes, the activation of NF-κB was increased and BAY 11-7082 could significantly improve the injury-induced swelling of astrocytes. After the injury of astrocytes, the activity of NOX was also increased, while BAY 11-7082 could reduce the activity of NOX.
  
  
  CONCLUSIONS
  The results show that the activation of NF-κB in astrocytes is a key factor in the process of cerebral edema after TBI of rats.
authors.affiliation: Department of Cerebral Surgery, Linyi People's Hospital, Shandong 276000, China.
authors.forename: Zi-Ran
authors.initials: ZR
authors.lastname: Wang
authors.affiliation: Department of Cerebral Surgery, Linyi People's Hospital, Shandong 276000, China.
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Li
authors.affiliation: Department of Cerebral Surgery, Linyi People's Hospital, Shandong 276000, China.
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Lei
authors.affiliation: Department of Cerebral Surgery, Linyi People's Hospital, Shandong 276000, China.
authors.forename: Dai-Qun
authors.initials: DQ
authors.lastname: Yang
authors.affiliation: Department of Cerebral Surgery, Linyi People's Hospital, Shandong 276000, China.
authors.forename: Li-Quan
authors.initials: LQ
authors.lastname: Wang
authors.affiliation: Department of Cerebral Surgery, Linyi People's Hospital, Shandong 276000, China. Electronic address: nyjzlhy@163.com.
authors.forename: Ming-Yu
authors.initials: MY
authors.lastname: Luo
keywords: Astrocytes; Nuclear transcription factor-κB; Rat; Traumatic brain injury