title: Bacurd1/Kctd13 and Bacurd2/Tnfaip1 are interacting partners to Rnd proteins which influence the long-term positioning and dendritic maturation of cerebral cortical neurons.
abstract: BACKGROUND
  The development of neural circuits within the embryonic cerebral cortex relies on the timely production of neurons, their positioning within the embryonic cerebral cortex as well as their terminal differentiation and dendritic spine connectivity. The RhoA GTPases Rnd2 and Rnd3 are important for neurogenesis and cell migration within the embryonic cortex (Nat Commun 4:1635, 2013), and we recently identified the BTB/POZ domain-containing Adaptor for Cul3-mediated RhoA Degradation family member Bacurd2 (also known as Tnfaip1) as an interacting partner to Rnd2 for the migration of embryonic mouse cortical neurons (Neural Dev 10:9, 2015).
  
  
  RESULTS
  We have extended this work and report that Bacurd1/Kctd13 and Bacurd2/Tnfaip1 are interacting partners to Rnd2 and Rnd3 in vitro. Given that these genes are expressed during cortical development, we performed a series of in utero electroporation studies in mice and found that disruptions to Bacurd1/Kctd13 or Bacurd2/Tnfaip1 expression impair the long-term positioning of E14.5-born cortical neurons within the postnatal (P17) mouse cerebral cortex. We also find that forced expression of Bacurd1/Kctd13 and Bacurd2/Tnfaip1 alters the branching and dendritic spine properties of layer II/III projection neurons.
  
  
  CONCLUSIONS
  We identify Bacurd1/Kctd13 and Bacurd2/Tnfaip1 as interacting partners to Rnd proteins which influence the development of cortical neurons. Their neurodevelopmental functions are likely to be relevant to human brain development and disease.
authors.affiliation: EMBL-Australia, The Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, VIC, 3800, Australia.|The Harry Perkins Institute of Medical Research, 6 Verdun St, Crawley, WA, 6009, Australia.|The Centre for Medical Research, The University of Western Australia, Crawley Avenue, Crawley, WA, 6009, Australia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Gladwyn-Ng
authors.affiliation: EMBL-Australia, The Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, VIC, 3800, Australia.
authors.forename: Lieven
authors.initials: L
authors.lastname: Huang
authors.affiliation: EMBL-Australia, The Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, VIC, 3800, Australia.|The Harry Perkins Institute of Medical Research, 6 Verdun St, Crawley, WA, 6009, Australia.|The Centre for Medical Research, The University of Western Australia, Crawley Avenue, Crawley, WA, 6009, Australia.
authors.forename: Linh
authors.initials: L
authors.lastname: Ngo
authors.affiliation: EMBL-Australia, The Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, VIC, 3800, Australia.
authors.forename: Shan Shan
authors.initials: SS
authors.lastname: Li
authors.affiliation: EMBL-Australia, The Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, VIC, 3800, Australia.
authors.forename: Zhengdong
authors.initials: Z
authors.lastname: Qu
authors.affiliation: The Harry Perkins Institute of Medical Research, 6 Verdun St, Crawley, WA, 6009, Australia.|The Centre for Medical Research, The University of Western Australia, Crawley Avenue, Crawley, WA, 6009, Australia.
authors.forename: Hannah Kate
authors.initials: HK
authors.lastname: Vanyai
authors.affiliation: The Harry Perkins Institute of Medical Research, 6 Verdun St, Crawley, WA, 6009, Australia.|The Centre for Medical Research, The University of Western Australia, Crawley Avenue, Crawley, WA, 6009, Australia.
authors.forename: Hayley Daniella
authors.initials: HD
authors.lastname: Cullen
authors.affiliation: EMBL-Australia, The Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, VIC, 3800, Australia.
authors.forename: John Michael
authors.initials: JM
authors.lastname: Davis
authors.affiliation: EMBL-Australia, The Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, VIC, 3800, Australia. julian.heng@perkins.uwa.edu.au.|The Harry Perkins Institute of Medical Research, 6 Verdun St, Crawley, WA, 6009, Australia. julian.heng@perkins.uwa.edu.au.|The Centre for Medical Research, The University of Western Australia, Crawley Avenue, Crawley, WA, 6009, Australia. julian.heng@perkins.uwa.edu.au.
authors.forename: Julian Ik-Tsen
authors.initials: JI
authors.lastname: Heng
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism* / Q000502:physiology; D002465:Cell Movement; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D049229:Dendritic Spines / Q000502:physiology*; D057809:HEK293 Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D011506:Proteins / Q000378:metabolism* / Q000502:physiology; D043743:Ubiquitin-Protein Ligase Complexes; D020741:rho GTP-Binding Proteins / Q000378:metabolism* / Q000502:physiology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; C485474:Tnfaip1 protein, mouse; C499862:PDIP1 protein, mouse; D043743:Ubiquitin-Protein Ligase Complexes; C475019:Rnd2 protein, mouse; C513341:Rnd3 protein, mouse; D020741:rho GTP-Binding Proteins
keywords: Bacurd; Dendritic branching; Dendritic spines; Neuronal migration