title: MGL2/YMR210w encodes a monoacylglycerol lipase in Saccharomyces cerevisiae.
abstract: In silico analysis of the uncharacterized open reading frame YMR210w in Saccharomyces cerevisiae revealed that it possesses both an α/β hydrolase domain (ABHD) and a typical lipase (GXSXG) motif. The purified protein displayed monoacylglycerol (MAG) lipase activity and preferred palmitoyl-MAG. Overexpression of YMR210w in the known MAG lipase mutant yju3Δ clearly revealed that the protein had MAG lipase activity, hence we named the ORF MGL2. Overexpression of YMR210w decreased the cellular triacylglycerol levels. Analysis of the overexpressed strains showed reduction in the lipid droplets number and size. Phenotype studies revealed that the double deletion yju3Δmgl2Δ displayed a growth defect that was partially restored by MGL2 overexpression.
authors.affiliation: Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
authors.forename: Kandasamy
authors.initials: K
authors.lastname: Selvaraju
authors.affiliation: Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
authors.forename: Ramachandran
authors.initials: R
authors.lastname: Gowsalya
authors.affiliation: Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
authors.forename: Rajendran
authors.initials: R
authors.lastname: Vijayakumar
authors.affiliation: Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
authors.forename: Vasanthi
authors.initials: V
authors.lastname: Nachiappan
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D002250:Carbon Radioisotopes; D005816:Genetic Complementation Test; D066292:Lipid Droplets / Q000378:metabolism; D054878:Lipoylation; D008563:Membrane Lipids / Q000378:metabolism; D050296:Microbial Viability; D008994:Monoacylglycerol Lipases / Q000737:chemistry / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D009154:Mutation / Q000235:genetics; D010743:Phospholipids / Q000378:metabolism; D010802:Phylogeny; D011994:Recombinant Proteins / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D013194:Staining and Labeling
chemical_list: D002250:Carbon Radioisotopes; D008563:Membrane Lipids; D010743:Phospholipids; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; C000619074:MGL2 protein, S cerevisiae; D008994:Monoacylglycerol Lipases
keywords: lipase; lipid droplet; lipid metabolism; monoacylglycerol; neutral lipid; αβ hydrolase