title: Multispectral Imaging of T and B Cells in Murine Spleen and Tumor.
abstract: Recent advances in multiplex immunohistochemistry techniques allow for quantitative, spatial identification of multiple immune parameters for enhanced diagnostic and prognostic insight. However, applying such techniques to murine fixed tissues, particularly sensitive epitopes, such as CD4, CD8α, and CD19, has been difficult. We compared different fixation protocols and Ag-retrieval techniques and validated the use of multiplex immunohistochemistry for detection of CD3(+)CD4(+) and CD3(+)CD8(+) T cell subsets in murine spleen and tumor. This allows for enumeration of these T cell subsets within immune environments, as well as the study of their spatial distribution.
authors.affiliation: Robert W. Franz Cancer Research Center, Earle A. Chiles Research Institute, Providence Cancer Center, Providence Portland Medical Center, Portland, OR 97213; Department of Cell, Developmental & Cancer Biology, Oregon Health & Science University, Portland, OR 97239;
authors.forename: Zipei
authors.identifier: 0000-0003-1396-9227
authors.initials: Z
authors.lastname: Feng
authors.affiliation: Robert W. Franz Cancer Research Center, Earle A. Chiles Research Institute, Providence Cancer Center, Providence Portland Medical Center, Portland, OR 97213;
authors.forename: Shawn M
authors.identifier: 0000-0002-4843-5930
authors.initials: SM
authors.lastname: Jensen
authors.affiliation: Robert W. Franz Cancer Research Center, Earle A. Chiles Research Institute, Providence Cancer Center, Providence Portland Medical Center, Portland, OR 97213; Department of Molecular Microbiology & Immunology, Oregon Health & Science University, Portland, OR 97239; and.
authors.forename: David J
authors.initials: DJ
authors.lastname: Messenheimer
authors.affiliation: Robert W. Franz Cancer Research Center, Earle A. Chiles Research Institute, Providence Cancer Center, Providence Portland Medical Center, Portland, OR 97213; Department of Cell, Developmental & Cancer Biology, Oregon Health & Science University, Portland, OR 97239;
authors.forename: Mohammad
authors.identifier: 0000-0002-3512-7509
authors.initials: M
authors.lastname: Farhad
authors.affiliation: Robert W. Franz Cancer Research Center, Earle A. Chiles Research Institute, Providence Cancer Center, Providence Portland Medical Center, Portland, OR 97213;
authors.forename: Michael
authors.identifier: 0000-0001-8453-5325
authors.initials: M
authors.lastname: Neuberger
authors.affiliation: Department of Pathology, Providence Portland Medical Center, Portland, OR 97213.
authors.forename: Carlo B
authors.initials: CB
authors.lastname: Bifulco
authors.affiliation: Robert W. Franz Cancer Research Center, Earle A. Chiles Research Institute, Providence Cancer Center, Providence Portland Medical Center, Portland, OR 97213; Department of Molecular Microbiology & Immunology, Oregon Health & Science University, Portland, OR 97239; and bernard.fox@providence.org.
authors.forename: Bernard A
authors.identifier: 0000-0002-4452-5947
authors.initials: BA
authors.lastname: Fox
mesh_terms: D000818:Animals; D018941:Antigens, CD19 / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology*; D017252:CD3 Complex / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D003952:Diagnostic Imaging / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D013154:Spleen / Q000378:metabolism* / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D018941:Antigens, CD19; D017252:CD3 Complex; D016827:CD8 Antigens