title: Multiplex real-time PCR for identification of canine parvovirus antigenic types.
abstract: Canine parvovirus (CPV) is an important disease causing gastroenteritis and/or haemorrhagic gastroenteritis in dogs. There are four antigenic types of CPV reported worldwide viz. CPV 2, CPV 2a, CPV 2b and CPV 2c. The diagnosis of CPV with the identification of the antigen type responsible remains problematic. In the present study, identification as well as antigenic typing of CPV was done using a de novo multiplex real time PCR to combat the problem of antigenic type identification. From the study it could be concluded that the here developed multiplex real time PCR assay could be used for rapid detection of CPV as well as typing of its three antigenic types.
authors.affiliation: Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141001, India. Electronic address: gurpreet7502@rediffmail.com.
authors.forename: Gurpreet
authors.initials: G
authors.lastname: Kaur
authors.affiliation: Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141001, India.
authors.forename: Mudit
authors.initials: M
authors.lastname: Chandra
authors.affiliation: Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141001, India.
authors.forename: P N
authors.initials: PN
authors.lastname: Dwivedi
authors.affiliation: Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141001, India.
authors.forename: Deepti
authors.initials: D
authors.lastname: Narang
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000175:diagnosis* / Q000821:virology*; D004285:Dogs; D005260:Female; D008297:Male; D060885:Multiplex Polymerase Chain Reaction; D010322:Parvoviridae Infections / Q000662:veterinary*; D017993:Parvovirus, Canine / Q000235:genetics* / Q000276:immunology; D060888:Real-Time Polymerase Chain Reaction
keywords: Antigenic types; Canine parvovirus; Multiplex real time PCR; VP2 gene