title: Affinity-based thermoresponsive precipitation of proteins modified with polymer-binding peptides.
abstract: A 12-mer peptide with an affinity for the meso diad sequence of poly(N-isopropylacrylamide) (PNIPAM) was identified through affinity-based peptide screening. A model protein (i.e., human serum albumin (HSA)) chemically modified with the peptide was successfully precipitated with PNIPAM above the lower critical solution temperature (LCST) of PNIPAM.
authors.affiliation: Department of Organic and Polymeric Materials, Tokyo Institute of Technology, 2-12-1-H121 Ookayama, Meguro-ku, Tokyo 152-8550, Japan. serizawa@polymer.titech.ac.jp.
authors.forename: Seigo
authors.initials: S
authors.lastname: Suzuki
authors.affiliation: Department of Organic and Polymeric Materials, Tokyo Institute of Technology, 2-12-1-H121 Ookayama, Meguro-ku, Tokyo 152-8550, Japan. serizawa@polymer.titech.ac.jp.
authors.forename: Toshiki
authors.initials: T
authors.lastname: Sawada
authors.affiliation: Department of Organic and Polymeric Materials, Tokyo Institute of Technology, 2-12-1-H121 Ookayama, Meguro-ku, Tokyo 152-8550, Japan. serizawa@polymer.titech.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ishizone
authors.affiliation: Department of Organic and Polymeric Materials, Tokyo Institute of Technology, 2-12-1-H121 Ookayama, Meguro-ku, Tokyo 152-8550, Japan. serizawa@polymer.titech.ac.jp.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Serizawa
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D020349:Surface Plasmon Resonance; D013696:Temperature
chemical_list: D000180:Acrylic Resins; D011506:Proteins; C052970:poly-N-isopropylacrylamide