title: Diterpenoids from the branch and leaf of Abies fargesii.
abstract: Five new abietane diterpenoids, named abifadines A-E (1-5), and nine known ones (6-14), together with two known podocarpenes (15 and 16), were isolated from the branch and leaf of Abies fargesii. Their structures including the relative configurations were established on the basis of extensive spectroscopic analyses. The absolute configuration of 1 was determined through single-crystal X-ray diffraction analysis with Cu Kα irradiation. All the isolates were evaluated for their in vitro cytotoxicities. Only compounds 5 and 12 exhibited weak anti-proliferative effects against three cancer cell lines (B16, MCF7, and HepG2) with IC50 values range from 14.8 to 42.8 μM. The antimicrobial activities against Staphylococcus aureus, Escherichia coli and Monilia albicans of all compounds were also tested, but none of them showed significant activities.
authors.affiliation: Faculty of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology, Hangkonglu 13, Wuhan 430030, People's Republic of China.
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Wu
authors.affiliation: Faculty of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology, Hangkonglu 13, Wuhan 430030, People's Republic of China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Faculty of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology, Hangkonglu 13, Wuhan 430030, People's Republic of China.
authors.forename: Xu
authors.initials: X
authors.lastname: Chen
authors.affiliation: Faculty of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology, Hangkonglu 13, Wuhan 430030, People's Republic of China.
authors.forename: An
authors.initials: A
authors.lastname: Jin
authors.affiliation: Faculty of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology, Hangkonglu 13, Wuhan 430030, People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Faculty of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology, Hangkonglu 13, Wuhan 430030, People's Republic of China.
authors.forename: Tian
authors.initials: T
authors.lastname: Tian
authors.affiliation: Faculty of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology, Hangkonglu 13, Wuhan 430030, People's Republic of China. Electronic address: ruanhl@mails.tjmu.edu.cn.
authors.forename: Han-Li
authors.initials: HL
authors.lastname: Ruan
mesh_terms: D028202:Abies / Q000737:chemistry*; D045784:Abietanes / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification; D002176:Candida albicans / Q000187:drug effects; D045744:Cell Line, Tumor; D002681:China; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D051379:Mice; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D045784:Abietanes; D000890:Anti-Infective Agents; D000972:Antineoplastic Agents, Phytogenic
keywords: Abies fargesii; Abietane diterpenoids; Antimicrobial activity; Cytotoxicity; Single-crystal X-ray diffraction