title: Expression of Heme Oxygenase-1 and Leukemia Inhibitory Factor in Maternal Plasma and Placental Tissue in a Lipopolysaccharide-Induced Late Pregnancy Preterm Birth Mouse Model.
abstract: OBJECTIVE
  To investigate the expression of heme oxygenase-1 (HO-1) and leukemia inhibitory factor (LIF) in maternal plasma and placental tissue in intrauterine infection-induced preterm birth.
  
  
  METHODS
  Using a mouse model of intrauterine infection in preterm birth, we used magnetic beads to extract normal pregnant mouse spleen Treg cells, injecting them into lipopolysaccharide (LPS)-treated pregnant mice. The subjects were divided into 4 groups: control group, LPS group, LPS+PBS group, and LPS+Treg group. RT-PCR and Western blot were used to evaluate HO-1, LIF mRNA, and protein levels in the placenta. ELISA was used to detect these parameters in the peripheral blood of pregnant mice.
  
  
  RESULTS
  The expression of HO-1 and LIF in the placenta of the LPS group was significantly decreased when compared to that of the control group (p<0.05). Serum HO-1 and LIF levels were higher than in the control group (p<0.05). In the Treg cell-treated group placental tissue HO-1 and LIF expression were significantly higher than in the LPS group (p<0.05), and serum HO-1 and LIF expression were significantly lower than in the LPS group (p<0.05).
  
  
  CONCLUSIONS
  HO-1 and LIF participate with Treg cells in the maternal-fetal interface, producing a unique immune microenvironment.
authors.forename: Fan
authors.initials: F
authors.lastname: Wang
authors.forename: Mi
authors.initials: M
authors.lastname: Xiao
authors.forename: Xiao-Jie
authors.initials: XJ
authors.lastname: Lin
authors.forename: Siddiq
authors.initials: S
authors.lastname: Muhammad
authors.forename: Xian-Hua
authors.initials: XH
authors.lastname: Piao
authors.forename: Li
authors.initials: L
authors.lastname: Liu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D051547:Heme Oxygenase-1 / Q000097:blood* / Q000235:genetics; D053605:Leukemia Inhibitory Factor / Q000097:blood* / Q000235:genetics; D051379:Mice; D010920:Placenta / Q000737:chemistry / Q000378:metabolism*; D011247:Pregnancy; D047928:Premature Birth / Q000097:blood / Q000235:genetics / Q000378:metabolism*
chemical_list: D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D051547:Heme Oxygenase-1