title: Gene expression profile of Musculus longissimus dorsi in bulls of a Charolais × Holstein F2-cross with divergent intramuscular fat content.
abstract: Transcriptomes of Musculus longissimus dorsi (MLD) were compared between bulls from a F2-cross derived from Charolais and Holstein Friesian. Two groups of 10 bulls were selected which differed significantly in intramuscular fat (IMF) deposition despite standardized husbandry and feeding conditions and identical sires in both groups. Consequently, genetic factors underlying the different capability of IMF deposition should be identified. A total of 32 differentially expressed genes (DEGs) were found of which 11 were up-regulated and 21 were down-regulated in the high IMF group. Ingenuity Pathway Analysis (IPA) identified a gene network comprising DEGs with functions in carbohydrate metabolism, lipid metabolism and molecular transport. The data from this study were deposited in NCBI's Gene Expression Omnibus and are accessible through GEO Series accession number GSE75347. We provide here a dataset which is of potential value to dissect molecular pathways influencing differences in IMF deposition in crossbred cattle with standardized genetic background.
authors.affiliation: Institute for Muscle Biology and Growth, Leibniz Institute for Farm Animal Biology, D-18197 Dummerstorf, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Komolka
authors.affiliation: Institute for Genome Biology, Leibniz Institute for Farm Animal Biology, D-18197 Dummerstorf, Germany.
authors.forename: Siriluck
authors.initials: S
authors.lastname: Ponsuksili
authors.affiliation: Institute for Muscle Biology and Growth, Leibniz Institute for Farm Animal Biology, D-18197 Dummerstorf, Germany.
authors.forename: Elke
authors.initials: E
authors.lastname: Albrecht
authors.affiliation: Institute for Genome Biology, Leibniz Institute for Farm Animal Biology, D-18197 Dummerstorf, Germany.
authors.forename: Christa
authors.initials: C
authors.lastname: Kühn
authors.affiliation: Institute for Genome Biology, Leibniz Institute for Farm Animal Biology, D-18197 Dummerstorf, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Wimmers
authors.affiliation: Institute for Muscle Biology and Growth, Leibniz Institute for Farm Animal Biology, D-18197 Dummerstorf, Germany.
authors.forename: Steffen
authors.initials: S
authors.lastname: Maak
keywords: Cattle; Charolais; Holstein; Intramuscular fat; Microarray; Musculus longissimus dorsi