title: SPRY4 Intronic Transcript 1 Promotes Epithelial-Mesenchymal Transition Through Association with Snail1 in Osteosarcoma.
abstract: Osteosarcoma is an aggressive tumor and the most common malignancy of the skeleton. Due to pulmonary metastasis, the 5-year survival rate is still unsatisfactory. It has been reported that SPRY4 intronic transcript 1 (SPRY4-IT1) promotes cell growth, invasion, and inhibits apoptosis in several cancers. However, the role of SPRY4-IT1 in osteosarcoma remains unclear. In the present study, we investigated the role of SPRY4-IT1 in osteosarcoma cells. Loss- and gain-of-function assays demonstrated that SPRY4-IT1 promoted cell proliferation, migration, and invasion in osteosarcoma. Moreover, SPRY4-IT1 induced epithelial-mesenchymal transition phenotype in osteosarcoma cells. Subsequent investigations revealed that SPRY4-IT1 promoted migration and invasion through association with Snail1 and regulating its stability. Based on these findings, the SPRY4-IT1/Snail1/E-cadherin pathway may play a crucial role in promoting osteosarcoma metastasis. Thus, SPRY4-IT1 may be a potential target for new therapies of osteosarcoma.
authors.affiliation: Department of Orthopedics, The People's Hospital of Three Gorges University , Yichang, China .
authors.forename: Neng
authors.initials: N
authors.lastname: Ru
authors.affiliation: Department of Orthopedics, The People's Hospital of Three Gorges University , Yichang, China .
authors.forename: Jie
authors.initials: J
authors.lastname: Liang
authors.affiliation: Department of Orthopedics, The People's Hospital of Three Gorges University , Yichang, China .
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, The People's Hospital of Three Gorges University , Yichang, China .
authors.forename: Weifei
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Orthopedics, The People's Hospital of Three Gorges University , Yichang, China .
authors.forename: Feifan
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, The People's Hospital of Three Gorges University , Yichang, China .
authors.forename: Xinzong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, The People's Hospital of Three Gorges University , Yichang, China .
authors.forename: Yuanli
authors.initials: Y
authors.lastname: Du
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D058750:Epithelial-Mesenchymal Transition; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology*; D015398:Signal Transduction; D000071250:Snail Family Transcription Factors / Q000378:metabolism
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; C000604407:SNAI1 protein, human; C499038:SPRY4 protein, human; D000071250:Snail Family Transcription Factors