title: Antioxidant and antifungal activities of Camellia sinensis (L.) Kuntze leaves obtained by different forms of production.
abstract: The antioxidant and anticandidal activities of leaves obtained from Camellia sinensis by non-fermentation (green and white teas), semi-fermentation (red tea) and fermentation method (black tea) were investigated. It was evaluated the total phenolic content by Folin-Ciocalteau assay; antioxidant capacities were evaluated in vitro using DPPH and ABTS radicals, hypochlorous acid and superoxide anion scavenger assays, induced hemolysis, lipid peroxidation by conjugated diene formation and myeloperoxidase activity. Anticandidal activity was performed on three strains of Candida spp. The results showed that non-fermented teas have a higher concentration of phenolic compounds, and then presented the best inhibitory activity of AAPH-induced hemolysis, the best inhibition of conjugated diene formation and more pronounced antioxidant activity in all tests. The highest anticandidal activity was obtained from fermented tea, followed by non-fermented tea. These results indicate that the antioxidant activity demonstrated has no direct relation with the anticandidal activity.
authors.affiliation: Pharmacy Department, Faculdade Guairacá, Guarapuava, PR, Brazil.
authors.forename: L E A
authors.initials: LE
authors.lastname: Camargo
authors.affiliation: Pharmacy Department, Faculdade Guairacá, Guarapuava, PR, Brazil.
authors.forename: L S
authors.initials: LS
authors.lastname: Pedroso
authors.affiliation: Laboratory of Pharmaceutical Nanotechnology, Universidade Estadual do Centro-Oeste, Guarapuava, PR, Brazil.
authors.forename: S C
authors.initials: SC
authors.lastname: Vendrame
authors.affiliation: Laboratory of Pharmaceutical Nanotechnology, Universidade Estadual do Centro-Oeste, Guarapuava, PR, Brazil.
authors.forename: R M
authors.initials: RM
authors.lastname: Mainardes
authors.affiliation: Laboratory of Pharmaceutical Nanotechnology, Universidade Estadual do Centro-Oeste, Guarapuava, PR, Brazil.
authors.forename: N M
authors.initials: NM
authors.lastname: Khalil
mesh_terms: D000935:Antifungal Agents / Q000032:analysis / Q000494:pharmacology*; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D028241:Camellia sinensis / Q000737:chemistry; D005285:Fermentation; D006461:Hemolysis; D015227:Lipid Peroxidation; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000378:metabolism; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts; D018515:Plant Leaves / Q000737:chemistry; D013662:Tea / Q000737:chemistry
chemical_list: D000935:Antifungal Agents; D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts; D013662:Tea; C011471:folin; D009195:Peroxidase