title: [Optimization of extraction technology for salidroside, tyrosol, crenulatin and gallic acid in Rhodiolae Crenulatae Radix et Rhizoma with orthogonal test].
abstract: The extracting technology of salidroside, tyrosol, crenulatin and gallic acid from Rhodiolae Crenulatae Radix et Rhizoma was optimized. With extraction rate of salidroside, tyrosol, crenulatin and gallic acid as indexes, orthogonal test was used to evaluate effect of 4 factors on extracting technology, including concentration of solvent, the dosage of solvent, duration of extraction, and frequency of extraction. The results showed that, the best extracting technology was to extract in 70% alcohol with 8 times the weight of herbal medicine for 2 times, with 3 hours once. High extraction rate of salidroside, tyrosol, crenulatin and gallic acid were obtained with the present technology. The extracting technology was stable and feasible with high extraction rate of four compounds from Rhodiolae Crenulatae Radix et Rhizoma, it was suitable for industrial production.
authors.forename: Xin
authors.initials: X
authors.lastname: Luo
authors.forename: Xue-jing
authors.initials: XJ
authors.lastname: Wang
authors.forename: Yi-wu
authors.initials: YW
authors.lastname: Zhao
authors.forename: Wen-zhe
authors.initials: WZ
authors.lastname: Huang
authors.forename: Zhen-zhong
authors.initials: ZZ
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D003374:Coumarins / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification*; D005707:Gallic Acid / Q000302:isolation & purification*; D005960:Glucosides / Q000302:isolation & purification*; D010636:Phenols / Q000302:isolation & purification*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000302:isolation & purification; D027343:Rhizome / Q000737:chemistry*; D029771:Rhodiola / Q000737:chemistry*
chemical_list: D003374:Coumarins; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D010636:Phenols; C011867:4-hydroxyphenylethanol; C076945:crenulatin coumarin; D005707:Gallic Acid; C009172:rhodioloside; D010626:Phenylethyl Alcohol