title: Snowmelt-driven changes in dissolved organic matter and bacterioplankton communities in the Heilongjiang watershed of China.
abstract: Bacterioplankton plays a significant role in the circulation of materials and ecosystem function in the biosphere. Dissolved organic matter (DOM) from dead plant material and surface soil leaches into water bodies when snow melts. In our study, water samples from nine sampling sites along the Heilongjiang watershed were collected in February and June 2014 during which period snowmelt occurred. The goal of this study was to characterize changes in DOM and bacterioplankton community composition (BCC) associated with snowmelt, the effects of DOM, environmental and geographical factors on the distribution of BCC and interactions of aquatic bacterioplankton populations with different sources of DOM in the Heilongjiang watershed. BCC was measured by denaturing gradient gel electrophoresis (DGGE). DOM was measured by excitation-emission matrix (EEM) fluorescence spectroscopy. Bacterioplankton exhibited a distinct seasonal change in community composition due to snowmelt at all sampling points except for EG. Redundancy analysis (RDA) indicated that BCC was more closely related to DOM (Components 1 and 4, dissolved organic carbon, biochemical oxygen demand and chlorophyll a) and environmental factors (water temperature and nitrate nitrogen) than geographical factors. Furthermore, DOM had a greater impact on BCC than environmental factors (29.80 vs. 15.90% of the variation). Overall, spring snowmelt played an important role in altering the quality and quantity of DOM and BCC in the Heilongjiang watershed.
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Qiu
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Hongyang
authors.initials: H
authors.lastname: Cui
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Junqiu
authors.initials: J
authors.lastname: Wu
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Baijie
authors.initials: B
authors.lastname: Wang
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China. Electronic address: zhao1970yue@163.com.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Heilongjiang Province Environmental Monitoring Centre, Harbin 150056, China.
authors.forename: Jiming
authors.initials: J
authors.lastname: Li
authors.affiliation: Heilongjiang Province Environmental Monitoring Centre, Harbin 150056, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Jia
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China. Electronic address: weizm691120@163.com.
authors.forename: Zimin
authors.initials: Z
authors.lastname: Wei
mesh_terms: D059001:Aquatic Organisms; D001419:Bacteria / Q000254:growth & development*; D002244:Carbon / Q000032:analysis; D002681:China; D002734:Chlorophyll; D000077194:Chlorophyll A; D004784:Environmental Monitoring; D005618:Fresh Water / Q000382:microbiology*; D009584:Nitrogen / Q000032:analysis; D010933:Plankton / Q000254:growth & development*; D012621:Seasons; D012914:Snow / Q000737:chemistry*; D014871:Water Microbiology
chemical_list: D002734:Chlorophyll; D002244:Carbon; D009584:Nitrogen; D000077194:Chlorophyll A
keywords: Bacterioplankton community composition; Dissolved organic matter; Heilongjiang watershed; Spring snowmelt