title: Combined effects of low-level laser therapy and human bone marrow mesenchymal stem cell conditioned medium on viability of human dermal fibroblasts cultured in a high-glucose medium.
abstract: Low-level laser therapy (LLLT) exhibited biostimulatory effects on fibroblasts viability. Secretomes can be administered to culture mediums by using bone marrow mesenchymal stem cells conditioned medium (BM-MSCs CM). This study investigated the combined effects of LLLT and human bone marrow mesenchymal stem cell conditioned medium (hBM-MSCs CM) on the cellular viability of human dermal fibroblasts (HDFs), which was cultured in a high-glucose (HG) concentration medium. The HDFs were cultured either in a concentration of physiologic (normal) glucose (NG; 5.5 mM/l) or in HG media (15 mM/l) for 4 days. LLLT was performed with a continuous-wave helium-neon laser (632.8 nm, power density of 0.00185 W/cm(2) and energy densities of 0.5, 1, and 2 J/cm(2)). About 10% of hBM-MSCs CM was added to the HG HDF culture medium. The viability of HDFs was evaluated using dimethylthiazol-diphenyltetrazolium bromide (MTT) assay. A significantly higher cell viability was observed when laser of either 0.5 or 1 J/cm(2) was used to treat HG HDFs, compared to the control groups. The cellular viability of HG-treated HDFs was significantly lower compared to the LLLT + HG HDFs, hBM-MSCs CM-treated HG HDFs, and LLLT + hBM-MSCs CM-treated HG HDFs. In conclusion, hBM-MSCs CM or LLLT alone increased the survival of HG HDFs cells. However, the combination of hBM-MSCs CM and LLLT improved these results in comparison to the conditioned medium.
authors.affiliation: Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, 1985717443, 19395/4719, Tehran, Iran.
authors.forename: Farzane
authors.initials: F
authors.lastname: Hendudari
authors.affiliation: Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, 1985717443, 19395/4719, Tehran, Iran. piryae@sbmu.ac.ir.|Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. piryae@sbmu.ac.ir.
authors.forename: Abbas
authors.initials: A
authors.lastname: Piryaei
authors.affiliation: Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
authors.forename: Seyedeh-Nafiseh
authors.initials: SN
authors.lastname: Hassani
authors.affiliation: Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Hasan
authors.initials: H
authors.lastname: Darbandi
authors.affiliation: Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, 1985717443, 19395/4719, Tehran, Iran. mohbayat@sbmu.ac.ir.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Bayat
mesh_terms: D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D054020:Lasers, Gas / Q000627:therapeutic use*; D028022:Low-Level Light Therapy; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D017077:Culture Media, Conditioned; D005947:Glucose
keywords: Conditioned medium; Diabetes mellitus; Human bone marrow-mesenchymal stem cell; Human dermal fibroblasts; Hyperglycemia; Low-level laser therapy