title: [Application of Rapid PCR Technology on Authentication of Species in Panax Genus].
abstract: OBJECTIVE
  To establish an accurate, rapid and efficient method for authentication of Panax species by using PCR amplification of specific alleles.
  
  
  METHODS
  The samples of Panax species were collected for extracting the total DNA. matK sequence from the Panax species was amplified by PCR and sequenced directionally, and then aligned by using Clustal W. Specific primers were designed and amplified by two-steps PCR amplification method.
  
  
  RESULTS
  By optimizing the denatured and annealing temperature and time, cycle numbers, the rapid PCR methods for authentication of Panax species were established respectively. When SYBR Green I was added in the PCR product, strong green fluorescence was visualized under 365 nm UV lamp whereas adulterants were not.
  
  
  CONCLUSIONS
  The rapid PCR method can identify the Panax species rapidly. This study provides the technical support for authentication of Chinese medicinal materials.
authors.forename: Zhan-hu
authors.initials: ZH
authors.lastname: Cui
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Jing-jing
authors.initials: JJ
authors.lastname: Zhang
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.forename: Sheng-chen
authors.initials: SC
authors.lastname: Ding
authors.forename: Xian-zhang
authors.initials: XZ
authors.lastname: Huang
authors.forename: Chao
authors.initials: C
authors.lastname: Jiang
mesh_terms: D000483:Alleles; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques; D005894:Panax / Q000145:classification*; D010946:Plants, Medicinal / Q000145:classification*; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D018744:DNA, Plant