title: Transferase Activity of Lactobacillal and Bifidobacterial β-Galactosidases with Various Sugars as Galactosyl Acceptors.
abstract: The β-galactosidases from Lactobacillus reuteri L103 (Lreuβgal), Lactobacillus delbrueckii subsp. bulgaricus DSM 20081 (Lbulβgal), and Bifidobacterium breve DSM 20281 (Bbreβgal-I and Bbreβgal-II) were investigated in detail with respect to their propensity to transfer galactosyl moieties onto lactose, its hydrolysis products D-glucose and D-galactose, and certain sugar acceptors such as N-acetyl-D-glucosamine (GlcNAc), N-acetyl-D-galactosamine (GalNAc), and L-fucose (Fuc) under defined, initial velocity conditions. The rate constants or partitioning ratios (kNu/kwater) determined for these different acceptors (termed nucleophiles, Nu) were used as a measure for the ability of a certain substance to act as a galactosyl acceptor of these β-galactosidases. When using Lbulβgal or Bbreβgal-II, the galactosyl transfer to GlcNAc was 6 and 10 times higher than that to lactose, respectively. With lactose and GlcNAc used in equimolar substrate concentrations, Lbulβgal and Bbreβgal-II catalyzed the formation of N-acetyl-allolactosamine with the highest yields of 41 and 24%, respectively, as calculated from the initial GlcNAc concentration.
authors.affiliation: Food Biotechnology Laboratory, Department of Food Science and Technology, BOKU - University of Natural Resources and Life Sciences , Muthgasse 18, A-1190 Vienna, Austria.|Institute of Chemistry, University of the Philippines Los Baños, College , Laguna, Philippines.
authors.forename: Sheryl Lozel
authors.initials: SL
authors.lastname: Arreola
authors.affiliation: Food Biotechnology Laboratory, Department of Food Science and Technology, BOKU - University of Natural Resources and Life Sciences , Muthgasse 18, A-1190 Vienna, Austria.|Department of Veterinary Bioscience and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University , Chiang Mai, Thailand.
authors.forename: Montira
authors.initials: M
authors.lastname: Intanon
authors.affiliation: Food Biotechnology Laboratory, Department of Food Science and Technology, BOKU - University of Natural Resources and Life Sciences , Muthgasse 18, A-1190 Vienna, Austria.|Faculty of Science, Maejo University , Chiang Mai, Thailand.
authors.forename: Pairote
authors.initials: P
authors.lastname: Wongputtisin
authors.affiliation: Division of Organic Chemistry, Department of Chemistry, BOKU - University of Natural Resources and Life Sciences , Muthgasse 18, A-1190 Vienna, Austria.
authors.forename: Paul
authors.initials: P
authors.lastname: Kosma
authors.affiliation: Food Biotechnology Laboratory, Department of Food Science and Technology, BOKU - University of Natural Resources and Life Sciences , Muthgasse 18, A-1190 Vienna, Austria.
authors.forename: Dietmar
authors.initials: D
authors.lastname: Haltrich
authors.affiliation: Food Biotechnology Laboratory, Department of Food Science and Technology, BOKU - University of Natural Resources and Life Sciences , Muthgasse 18, A-1190 Vienna, Austria.
authors.forename: Thu-Ha
authors.initials: TH
authors.lastname: Nguyen
mesh_terms: D000116:Acetylgalactosamine / Q000031:analogs & derivatives / Q000378:metabolism / Q000493:pharmacokinetics; D000117:Acetylglucosamine / Q000378:metabolism / Q000493:pharmacokinetics; D001644:Bifidobacterium / Q000378:metabolism; D005688:Galactosamine / Q000378:metabolism; D005690:Galactose / Q000378:metabolism*; D005944:Glucosamine / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007778:Lactobacillus / Q000201:enzymology*; D052200:Limosilactobacillus reuteri / Q000378:metabolism; D007785:Lactose / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism*; D013379:Substrate Specificity; D014166:Transferases / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D009844:Oligosaccharides; D005688:Galactosamine; D014166:Transferases; D001616:beta-Galactosidase; D005947:Glucose; D007785:Lactose; D000116:Acetylgalactosamine; D005944:Glucosamine; D000117:Acetylglucosamine; D005690:Galactose
keywords: galacto-oligosaccharides; galactosyl acceptors; hetero-oligosaccharides; transgalactosylation; β-galactosidases