title: Isoprenaline Induces Periostin Expression in Gastric Cancer.
abstract: OBJECTIVE
  Periostin mediates critical steps in gastric cancer and is involved in various signaling pathways. However, the roles of periostin in promoting gastric cancer metastasis are not clear. The aim of this study was to investigate the relevance between periostin expression and gastric cancer progression and the role of stress-related hormones in the regulation of cancer development and progression.
  
  
  METHODS
  Normal, cancerous and metastatic gastric tissues were collected from patients diagnosed with advanced gastric cancer. The in vivo expression of periostin was evaluated by in situ hybridization and immunofluorescent staining. Meanwhile, human gastric adenocarcinoma cell lines MKN-45 and BGC-803 were used to detect the in vitro expression of periostin by using quantitative real-time polymerase chain reaction (PCR) and western blotting.
  
  
  RESULTS
  Periostin is expressed in the stroma of the primary gastric tumors and metastases, but not in normal gastric tissue. In addition, we observed that periostin is located mainly in pericryptal fibroblasts, but not in the tumor cells, and strongly correlated to the expression of α-smooth muscle actin (SMA). Furthermore, the distribution patterns of periostin were broader as the clinical staging of tumors progressed. We also identified a role of stress-related signaling in promoting cancer development and progression, and found that isoprenaline upregulated expression levels of periostin in gastric cancer cells.
  
  
  CONCLUSIONS
  These findings suggest that the distribution pattern of periostin was broader as the clinical staging of the tumor progressed and found that isoprenaline upregulated expression levels of periostin in gastric cancer cells.
authors.affiliation: Department of General Surgery, Chinese PLA General Hospital, Beijing, China.
authors.forename: Guo-Xiao
authors.initials: GX
authors.lastname: Liu
authors.affiliation: Department of General Surgery, Chinese PLA General Hospital, Beijing, China.
authors.forename: Hong-Qing
authors.initials: HQ
authors.lastname: Xi
authors.affiliation: Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Science, Trauma Center of Postgraduate Medical School, Chinese PLA General Hospital, Beijing, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Sun
authors.affiliation: Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Science, Trauma Center of Postgraduate Medical School, Chinese PLA General Hospital, Beijing, China.
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Geng
authors.affiliation: Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Science, Trauma Center of Postgraduate Medical School, Chinese PLA General Hospital, Beijing, China.
authors.forename: Shao-Wei
authors.initials: SW
authors.lastname: Yang
authors.affiliation: Department of Pathology, Chengde Medical College, Chengde, Hebei Province, China.
authors.forename: Yan-Jie
authors.initials: YJ
authors.lastname: Lu
authors.affiliation: Department of General Surgery, Chinese PLA General Hospital, Beijing, China. weibo@vip.163.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Wei
authors.affiliation: Department of General Surgery, Chinese PLA General Hospital, Beijing, China. 13381326128@163.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000368:Aged; D015153:Blotting, Western; D015815:Cell Adhesion Molecules / Q000187:drug effects / Q000378:metabolism*; D045744:Cell Line, Tumor; D005347:Fibroblasts / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D009367:Neoplasm Staging; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction; D013270:Stomach / Q000473:pathology; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D015854:Up-Regulation
chemical_list: D000318:Adrenergic beta-Agonists; D015815:Cell Adhesion Molecules; C512835:POSTN protein, human; D012333:RNA, Messenger; D007545:Isoproterenol
keywords: Gastric cancer; extracellular matrix; metastasis; nich; periostin; stress