title: Simple Purification of Adeno-Associated Virus-DJ for Liver-Specific Gene Expression.
abstract: Recombinant gene expression using adeno-associated viruses (AAVs) has become a valuable tool in animal studies, as they mediate safe expression of transduced genes for several months. The liver is a major organ of metabolism, and liver-specific expression of a gene can be an invaluable tool for metabolic studies. AAV-DJ is a recombinant AAV generated by the gene shuffling of various AAV serotypes and shares characteristics of AAV2 and AAV8. AAV-DJ contains a heparin-binding domain in its capsid, which suggests that a heparin column could be used for the purification of the AAV. Given that AAV-DJ has been only recently available, relatively little is known about the optimal preparation/purification and application of AAV-DJ. Here, we present a simple large-scale preparation method that can generate 3×10¹³ viral particles for in vivo experiments and demonstrate liver-specific gene expression via systemic injection in mice.
authors.affiliation: Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX, USA.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX, USA.|Department of Molecular Medicine, Inha University School of Medicine, Incheon, Korea. yamoon15@inha.ac.kr.
authors.forename: Young-Ah
authors.initials: YA
authors.lastname: Moon
mesh_terms: D000818:Animals; D002213:Capsid; D036022:Capsid Proteins / Q000235:genetics*; D000229:Dependovirus / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors; D016679:Genome, Viral / Q000235:genetics; D056945:Hep G2 Cells; D006801:Humans; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D036022:Capsid Proteins
keywords: AAV-DJ; large scale production; liver-specific expression