title: (1)H, (13)C and (15)N resonance assignments of the Cdc42-binding domain of TOCA1.
abstract: TOCA1 is a downstream effector protein of the small GTPase, Cdc42. It is a multi-domain protein that includes a membrane binding F-BAR domain, a homology region 1 (HR1) domain, which binds selectively to active Cdc42 and an SH3 domain. TOCA1 is involved in the regulation of actin dynamics in processes such as endocytosis, filopodia formation, neurite elongation, cell motility and invasion. Structural insight into the interaction between TOCA1 and Cdc42 will contribute to our understanding of the role of TOCA1 in actin dynamics. The (1)H, (15)N and (13)C NMR backbone and sidechain resonance assignment of the HR1 domain (12 kDa) presented here provides the foundation for structural studies of the domain and its interactions.
authors.affiliation: Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1GA, UK.
authors.forename: Joanna R
authors.initials: JR
authors.lastname: Watson
authors.affiliation: Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1GA, UK.
authors.forename: Daniel
authors.initials: D
authors.lastname: Nietlispach
authors.affiliation: Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1GA, UK.
authors.forename: Darerca
authors.initials: D
authors.lastname: Owen
authors.affiliation: Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1GA, UK. hrm28@bioc.cam.ac.uk.
authors.forename: Helen R
authors.identifier: 0000-0002-7890-7097
authors.initials: HR
authors.lastname: Mott
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism*; D050556:Fatty Acid-Binding Proteins; D019906:Nuclear Magnetic Resonance, Biomolecular; D000072417:Protein Domains; D017433:Protein Structure, Secondary; D014981:Xenopus; D029867:Xenopus Proteins / Q000737:chemistry* / Q000378:metabolism*; D020764:cdc42 GTP-Binding Protein / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; C488976:FNBP1L protein, Xenopus; D050556:Fatty Acid-Binding Proteins; D029867:Xenopus Proteins; D020764:cdc42 GTP-Binding Protein
keywords: Cdc42; Cytoskeleton; Endocytosis; GTPase; HR1; Signalling; TOCA1