title: Bimodal X-ray and Infrared Imaging of an Organometallic Derivative of Praziquantel in Schistosoma mansoni.
abstract: An organometallic derivative of praziquantel was studied directly in worms by using inductively coupled plasma-mass spectrometry (ICP-MS) for quantification and synchrotron-based imaging. X-ray fluorescence (XRF) and IR absorption spectromicroscopy were used for the first time in combination to directly locate this organometallic drug candidate in schistosomes. The detection of both CO (IR) and Cr (XRF) signatures proved that the Cr(CO)3 core remained intact in the worms. Images showed a preferential accumulation at the worm's tegument, consistent with a possible targeting of the calcium channel but not excluding other biological targets inside the worm.
authors.affiliation: École Normale Supérieure, PSL Research University, Département de Chimie, 24 rue Lhomond, 75005, Paris, France.|Sorbonne Universités, UPMC Univ Paris 06, LBM, 4 place Jussieu, 75005, Paris, France.|Centre National de la Recherche Scientifique (CNRS), UMR 7203 LBM, 75005, Paris, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Clède
authors.affiliation: Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute Basel, Switzerland, University of Basel, P. O. Box, 4003, Basel, Switzerland.
authors.forename: Noemi
authors.initials: N
authors.lastname: Cowan
authors.affiliation: École Normale Supérieure, PSL Research University, Département de Chimie, 24 rue Lhomond, 75005, Paris, France.|Sorbonne Universités, UPMC Univ Paris 06, LBM, 4 place Jussieu, 75005, Paris, France.|Centre National de la Recherche Scientifique (CNRS), UMR 7203 LBM, 75005, Paris, France.
authors.forename: François
authors.initials: F
authors.lastname: Lambert
authors.affiliation: École Normale Supérieure, PSL Research University, Département de Chimie, 24 rue Lhomond, 75005, Paris, France.|Sorbonne Universités, UPMC Univ Paris 06, LBM, 4 place Jussieu, 75005, Paris, France.|Centre National de la Recherche Scientifique (CNRS), UMR 7203 LBM, 75005, Paris, France.
authors.forename: Hélène C
authors.initials: HC
authors.lastname: Bertrand
authors.affiliation: Department of Chemistry, University of Zürich, Winterthurerstrasse 190, 8057, Zürich, Switzerland.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Rubbiani
authors.affiliation: Department of Chemistry, University of Zürich, Winterthurerstrasse 190, 8057, Zürich, Switzerland.
authors.forename: Malay
authors.initials: M
authors.lastname: Patra
authors.affiliation: Department of Chemistry, University of Zürich, Winterthurerstrasse 190, 8057, Zürich, Switzerland.
authors.forename: Jeannine
authors.initials: J
authors.lastname: Hess
authors.affiliation: Synchrotron SOLEIL, L'Orme des Merisiers, Saint Aubin, B. P. 48, 91192, Gif-sur-Yvette, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Sandt
authors.affiliation: Synchrotron SOLEIL, L'Orme des Merisiers, Saint Aubin, B. P. 48, 91192, Gif-sur-Yvette, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Trcera
authors.affiliation: Department of Chemistry, University of Zürich, Winterthurerstrasse 190, 8057, Zürich, Switzerland. gilles.gasser@chem.uzh.ch.
authors.forename: Gilles
authors.initials: G
authors.lastname: Gasser
authors.affiliation: Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute Basel, Switzerland, University of Basel, P. O. Box, 4003, Basel, Switzerland. jennifer.keiser@unibas.ch.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Keiser
authors.affiliation: École Normale Supérieure, PSL Research University, Département de Chimie, 24 rue Lhomond, 75005, Paris, France. clotilde.policar@ens.fr.|Sorbonne Universités, UPMC Univ Paris 06, LBM, 4 place Jussieu, 75005, Paris, France. clotilde.policar@ens.fr.|Centre National de la Recherche Scientifique (CNRS), UMR 7203 LBM, 75005, Paris, France. clotilde.policar@ens.fr.
authors.forename: Clotilde
authors.initials: C
authors.lastname: Policar
mesh_terms: D000818:Animals; D002857:Chromium / Q000737:chemistry; D013058:Mass Spectrometry; D008853:Microscopy; D061848:Optical Imaging; D011223:Praziquantel / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D012550:Schistosoma mansoni / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D013055:Spectrophotometry, Infrared; D013237:Stereoisomerism; D056928:X-Ray Absorption Spectroscopy
chemical_list: D002857:Chromium; D011223:Praziquantel
keywords: IR absorption spectroscopy; X-ray fluorescence; chromium; organometallic chemistry; schistosomes