title: Cloning and Transformation of EeHKT1;4 Gene from Elytrigia elongata.
abstract: The EeHKT1;4 gene was firstly cloned from Elytrigia elongata by RT-PCR technique with 1977 bp full-length cDNA encoding 1722 bp open reading frame (ORF) and 573 amino acids. The PCR fragment of EeHKT1;4 gene was inserted into the binary vector pBI121 and got the resulted expression vector, which named pBI121-35S-EeHKT1;4-Nos. The vector was further transformed into the agrobacterium EHA105, and then EeHKT1;4 gene was transferred into tobacco by the Agrobaterium- mediated genetic transformation method. The results showed that the target gene was inserted into the genomes of tobacco and expressed. Therefore, the transgenic tobacco (T0) plants overexpressing EeHKT1;4 gene were successfully obtained in this study. And EeHKT1;4 reduces Na+ concentration in the leaves of T0 plants, thereby plays a central role in protecting plant leaves from salinity stress.
authors.affiliation: Beijing Research and Development Center for Grass and Environment, Beijing Academy of Agriculture and Forestry Sciences, Haidian District, Beijing, 100097, China. menglin9599@sina.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Meng
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Guo
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Li
authors.forename: Pei-Chun
authors.initials: PC
authors.lastname: Mao
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Tian
mesh_terms: D027682:Cation Transport Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D006109:Poaceae / Q000235:genetics*; D027981:Symporters / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D027682:Cation Transport Proteins; C088864:HKT1 protein, plant; D010940:Plant Proteins; D027981:Symporters