title: Single-Stranded Tile Stoppers for Interlocked DNA Architectures.
abstract: Interlocked DNA architectures are useful for DNA nanotechnology because of their mechanically bonded components, which can move relative to one another without disassembling. We describe the design, synthesis, and characterization of novel single-stranded tile (SST) stoppers for the assembly of interlocked DNA architectures. SST stoppers are shown to self-assemble into a square-shaped rigid structure upon mixing 97 oligodeoxynucleotide (ODN) strands. The structures are equipped with a sticky end that is designed for hybridization with the sticky ends of a dsDNA axle of a DNA rotaxane. Because the diameter of the macrocycle threaded onto the axle is 14 nm, the dimension of the square-shaped stopper was designed to be bulky enough to prevent the dethreading of the macrocycle. An asymmetric rotaxane with a SST- and a ring-shaped stopper featuring two stations for hybridization of the macrocycle to the axle was assembled. The macrocycle can be directed towards one or the other station upon triggering with fuel ODNs.
authors.affiliation: Life and Medical Science (LIMES) Institute, Chemical Biology & Medicinal Chemistry Unit, University of Bonn, Gerhard-Domagk Strasse 1, 53121, Bonn, Germany.
authors.forename: Julián
authors.initials: J
authors.lastname: Valero
authors.affiliation: Life and Medical Science (LIMES) Institute, Chemical Biology & Medicinal Chemistry Unit, University of Bonn, Gerhard-Domagk Strasse 1, 53121, Bonn, Germany.
authors.forename: Finn
authors.initials: F
authors.lastname: Lohmann
authors.affiliation: Life and Medical Science (LIMES) Institute, Chemical Biology & Medicinal Chemistry Unit, University of Bonn, Gerhard-Domagk Strasse 1, 53121, Bonn, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Keppner
authors.affiliation: Life and Medical Science (LIMES) Institute, Chemical Biology & Medicinal Chemistry Unit, University of Bonn, Gerhard-Domagk Strasse 1, 53121, Bonn, Germany. m.famulok@uni-bonn.de.|Center of Advanced European Studies and Research (CAESAR), Ludwig-Erhard-Allee 2, 53175, Bonn, Germany. m.famulok@uni-bonn.de.
authors.forename: Michael
authors.initials: M
authors.lastname: Famulok
mesh_terms: D004277:DNA, Single-Stranded / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D043862:Rotaxanes / Q000737:chemistry
chemical_list: D004277:DNA, Single-Stranded; D043862:Rotaxanes
keywords: DNA rotaxanes; DNA structures; interlocked DNA structures; nanotechnology; single-stranded tiles (SST)