title: Endophytic fungus strain 28 isolated from Houttuynia cordata possesses wide-spectrum antifungal activity.
abstract: The aim of this paper is to identify and investigate an endophytic fungus (strain 28) that was isolated from Houttuynia cordata Thunb, a famous and widely-used Traditional Chinese Medicine. Based on morphological methods and a phylogenetic analysis of ITS sequences, this strain was identified as Chaetomium globosum. An antifungal activity bioassay demonstrated that the crude ethyl acetate (EtOAc) extracts of strain 28 had a wide antifungal spectrum and strong antimicrobial activity, particularly against Exserohilum turcicum (Pass.) Leonard et Suggs, Botrytis cinerea persoon and Botrytis cinerea Pers. ex Fr. Furthermore, the fermentation conditions, extraction method and the heat stability of antifungal substances from strain 28 were also studied. The results showed that optimal antifungal activity can be obtained with the following parameters: using potato dextrose broth (PDB) as the base culture medium, fermentation for 4-8d (initial pH: 7.5), followed by extraction with EtOAc. The extract was stable at temperatures up to 80°C. This is the first report on the isolation of endophytic C. globosum from H. cordata to identify potential alternative biocontrol agents that could provide new opportunities for practical applications involving H. cordata.
authors.affiliation: Agronomy College, Sichuan Agricultural University, Chengdu, Sichuan, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Pan
authors.affiliation: Agronomy College, Sichuan Agricultural University, Chengdu, Sichuan, PR China.
authors.forename: Zheng-Qiong
authors.initials: ZQ
authors.lastname: Liu
authors.affiliation: Agronomy College, Sichuan Agricultural University, Chengdu, Sichuan, PR China.
authors.forename: Que
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Agronomy College, Sichuan Agricultural University, Chengdu, Sichuan, PR China.
authors.forename: Ying-Wen
authors.initials: YW
authors.lastname: Xu
authors.affiliation: Agronomy College, Sichuan Agricultural University, Chengdu, Sichuan, PR China.
authors.forename: Kai
authors.initials: K
authors.lastname: Hou
authors.affiliation: Agronomy College, Sichuan Agricultural University, Chengdu, Sichuan, PR China. Electronic address: ewuwei@gmail.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
mesh_terms: D000935:Antifungal Agents / Q000378:metabolism* / Q000494:pharmacology; D002597:Chaetomium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D060026:Endophytes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005658:Fungi / Q000187:drug effects / Q000254:growth & development; D052859:Houttuynia / Q000382:microbiology*; D010802:Phylogeny
chemical_list: D000935:Antifungal Agents
keywords: Antifungal activity; Chaetomium globosum; Endophytic fungi; Houttuynia cordata Thunb.; Process parameter