title: Simultaneous determination of 8-oxo-2'-deoxyguanosine and 8-oxo-2'-deoxyadenosine in human retinal DNA by liquid chromatography nanoelectrospray-tandem mass spectrometry.
abstract: Age-related macular degeneration (AMD) is the leading cause of blindness among older adults in the developed world. Oxidative damage to mitochondrial DNA (mtDNA) in the retinal pigment epithelium (RPE) may play a key role in AMD. Measurement of oxidative DNA lesions such as 8-oxo-2'-deoxyguanosine (8-oxo-dG) and 8-oxo-2'-deoxyadenosine (8-oxo-dA) in diseased RPE could provide important insights into the mechanism of AMD development. We have developed a liquid chromatography-nanoelectrospray ionization-tandem mass spectrometry method for simultaneous analysis of 8-oxo-dG and 8-oxo-dA in human retinal DNA. The developed method was applied to the analysis of retinal DNA from 5 donors with AMD and 5 control donors without AMD. In mtDNA, the levels of 8-oxo-dG in controls and AMD donors averaged 170 and 188, and 8-oxo-dA averaged 11 and 17 adducts per 10(6) bases, respectively. In nuclear DNA, the levels of 8-oxo-dG in controls and AMD donors averaged 0.54 and 0.96, and 8-oxo-dA averaged 0.04 and 0.05 adducts per 10(6) bases, respectively. This highly sensitive method allows for the measurement of both adducts in very small amounts of DNA and can be used in future studies investigating the pathophysiological role of 8-oxo-dG and 8-oxo-dA in AMD and other oxidative damage-related diseases in humans.
authors.affiliation: Masonic Cancer Center, University of Minnesota, Mayo Mail Code 806, 420 Delaware Street SE, Minneapolis, Minnesota 55455, United States.
authors.forename: Bin
authors.initials: B
authors.lastname: Ma
authors.affiliation: Masonic Cancer Center, University of Minnesota, Mayo Mail Code 806, 420 Delaware Street SE, Minneapolis, Minnesota 55455, United States.
authors.forename: Meng
authors.initials: M
authors.lastname: Jing
authors.affiliation: Masonic Cancer Center, University of Minnesota, Mayo Mail Code 806, 420 Delaware Street SE, Minneapolis, Minnesota 55455, United States.
authors.forename: Peter W
authors.initials: PW
authors.lastname: Villalta
authors.affiliation: Department of Ophthalmology and Visual Neurosciences, University of Minnesota, Mayo Mail Code 493, 420 Delaware Street SE, Minneapolis, Minnesota 55455, United States.
authors.forename: Rebecca J
authors.initials: RJ
authors.lastname: Kapphahn
authors.affiliation: Department of Ophthalmology and Visual Neurosciences, University of Minnesota, Mayo Mail Code 493, 420 Delaware Street SE, Minneapolis, Minnesota 55455, United States.
authors.forename: Sandra R
authors.initials: SR
authors.lastname: Montezuma
authors.affiliation: Department of Ophthalmology and Visual Neurosciences, University of Minnesota, Mayo Mail Code 493, 420 Delaware Street SE, Minneapolis, Minnesota 55455, United States.
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Ferrington
authors.affiliation: Masonic Cancer Center, University of Minnesota, Mayo Mail Code 806, 420 Delaware Street SE, Minneapolis, Minnesota 55455, United States.|Division of Environmental Health Sciences, University of Minnesota, Mayo Mail Code 807, 420 Delaware Street SE, Minneapolis, Minnesota 55455, United States.
authors.forename: Irina
authors.initials: I
authors.lastname: Stepanov
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D002853:Chromatography, Liquid / Q000379:methods*; D004249:DNA Damage; D004272:DNA, Mitochondrial / Q000235:genetics; D003839:Deoxyadenosines / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008268:Macular Degeneration / Q000175:diagnosis / Q000235:genetics; D015394:Molecular Structure; D036103:Nanotechnology / Q000379:methods; D015203:Reproducibility of Results; D055213:Retinal Pigment Epithelium / Q000378:metabolism* / Q000473:pathology; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004272:DNA, Mitochondrial; D003839:Deoxyadenosines; C078251:2'-deoxy-7,8-dihydro-8-oxoadenosine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine