title: Bacteriophage application to control the contaminated water with Shigella.
abstract: Shigella is one of the most important waterborne and foodborne pathogens around the world. Emergence of antibiotic-resistant Shigella has made the development of alternatives to conventional antibiotics necessary. In this study, a virulent Myoviridae bacteriophage, pSs-1 was isolated from environmental water in South Korea and showed infectivity to S. flexneri as well as S. sonnei strains. One-step growth analysis showed that pSs-1 has a short latent period (25 min) and a large burst size (97 PFU/cell). According to the genomic analysis, pSs-1 contains 164,999 bp of genome with a G + C content of 35.54% and it is considered as a member of the T4-like bacteriophage group. These results showed that pSs-1 may have potential as a biocontrol agent instead of conventional antibiotics for shigellosis.
authors.affiliation: College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, Republic of Korea.
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Jun
authors.affiliation: College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, Republic of Korea.
authors.forename: Sib Sankar
authors.initials: SS
authors.lastname: Giri
authors.affiliation: College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, Republic of Korea.
authors.forename: Hyoun Joong
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, Republic of Korea.
authors.forename: Sae Kil
authors.initials: SK
authors.lastname: Yun
authors.affiliation: College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, Republic of Korea.
authors.forename: Cheng
authors.initials: C
authors.lastname: Chi
authors.affiliation: Departments of Rheumatology, Bundang Jesaeng Hospital, Seongnam, Republic of Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Chai
authors.affiliation: College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, Republic of Korea.
authors.forename: Byeong Chun
authors.initials: BC
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, Republic of Korea.
authors.forename: Se Chang
authors.initials: SC
authors.lastname: Park
mesh_terms: D001482:Base Composition; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics; D016679:Genome, Viral / Q000235:genetics; D054884:Host-Pathogen Interactions; D008854:Microscopy, Electron; D017900:Myoviridae / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012763:Shigella flexneri / Q000821:virology*; D012764:Shigella sonnei / Q000821:virology*; D013045:Species Specificity; D013997:Time Factors; D017735:Virus Latency; D014871:Water Microbiology; D014876:Water Pollution / Q000517:prevention & control*
chemical_list: D004279:DNA, Viral