title: Bevacizumab and cetuximab with conventional chemotherapy reduced pancreatic tumor weight in mouse pancreatic cancer xenografts.
abstract: Pancreatic cancer remains the fourth leading cause of cancer-related death in the USA with a 5-year survival rate of 5 %. The effects of epidermal growth factor receptor and vascular endothelial growth factor A blockade with chemotherapy on pancreatic tumor growth were examined. Mice bearing human PANC-1 cell xenografts were divided into three groups: T-CR (gemcitabine, cisplatin, and 5-fluorouracil), T-TR (cetuximab, bevacizumab, gemcitabine, cisplatin, and 5-fluorouracil), and vehicle control (T). The therapies were administered via intraperitoneal injections every 4 days for seven cycles from 7 weeks after cancer cell implantation. Mice treated with T-TR had significant reductions in tumor weight as compared to the control group (p < 0.05). Although mice in the T-CR group experienced a significant reduction in body weight gain, serum albumin, and gastrocnemius muscle mass (p < 0.05), no such reductions were observed in the T-TR group. Mice treated with T-TR had slightly increased CD11c+DC and CD49b+NK cell levels in the spleen (p < 0.05) and significantly lower tumor VEGF expression (p < 0.05). Tumor carcinoembryonic antigen expression was significantly reduced in both treatment groups (p < 0.05). Thus, addition of bevacizumab and cetuximab to gemcitabine, cisplatin, and fluorouracil may represent an effective treatment option for pancreatic cancer that warrants further study.
authors.affiliation: Division of Hematology and Oncology, Department of Internal Medicine, Taipei Medical University Hospital, No. 252, Wuxing St., Taipei, 11031, Taiwan. cjtai@tmu.edu.tw.|Division of Hematology and Oncology, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, 11031, Taiwan. cjtai@tmu.edu.tw.
authors.forename: Cheng-Jeng
authors.initials: CJ
authors.lastname: Tai
authors.affiliation: Institute of Biomedical Nutrition, Hung Kuang University, Taichung, 43302, Taiwan.
authors.forename: Hang
authors.initials: H
authors.lastname: Wang
authors.affiliation: Division of Hematology and Oncology, Department of Internal Medicine, Taipei Medical University Hospital, No. 252, Wuxing St., Taipei, 11031, Taiwan.|Division of Hematology and Oncology, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, 11031, Taiwan.|Department of Obstetrics and Gynecology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, 11031, Taiwan.|Department of Chinese Medicine, Taipei Medical University Hospital, Taipei, 11031, Taiwan.
authors.forename: Chien-Kai
authors.initials: CK
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, 11031, Taiwan.|Department of Chinese Medicine, Taipei Medical University Hospital, Taipei, 11031, Taiwan.|Traditional Herbal Medicine Research Center, Taipei Medical University Hospital, Taipei City, 11031, Taiwan.
authors.forename: Chen-Jei
authors.initials: CJ
authors.lastname: Tai
authors.affiliation: Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, 11031, Taiwan.|Division of General Surgery, Department of Surgery, Shuang Ho Hospital, Taipei Medical University, Taipei, 23561, Taiwan.
authors.forename: Ming-Te
authors.initials: MT
authors.lastname: Huang
authors.affiliation: Division of General Surgery, Department of Surgery, Shuang Ho Hospital, Taipei Medical University, Taipei, 23561, Taiwan.|Center of Excellence for Cancer Research, Taipei Medical University, Taipei, 11031, Taiwan.|Department of Surgery, Taipei Medical University-Shuang Ho Hospital, Taipei, 23561, Taiwan.
authors.forename: Chih-Hsiung
authors.initials: CH
authors.lastname: Wu
authors.affiliation: Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, 11031, Taiwan.|Division of General Surgery, Department of Surgery, Shuang Ho Hospital, Taipei Medical University, Taipei, 23561, Taiwan.
authors.forename: Ray-Jade
authors.initials: RJ
authors.lastname: Chen
authors.affiliation: Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, 11031, Taiwan.|Division of General Surgery, Department of Surgery, Shuang Ho Hospital, Taipei Medical University, Taipei, 23561, Taiwan.
authors.forename: Li-Jen
authors.initials: LJ
authors.lastname: Kuo
authors.affiliation: Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, 11031, Taiwan.|Division of General Surgery, Department of Surgery, Shuang Ho Hospital, Taipei Medical University, Taipei, 23561, Taiwan.
authors.forename: Po-Lei
authors.initials: PL
authors.lastname: Wei
authors.affiliation: Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, 11031, Taiwan.|Cancer Research Center, Taipei Medical University and Hospital, Taipei, 11031, Taiwan.|Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, 11031, Taiwan.
authors.forename: Yu-Jia
authors.initials: YJ
authors.lastname: Chang
authors.affiliation: Division of Hematology and Oncology, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, 11031, Taiwan.|Division of Gastroenterology, Department of Internal Medicine, Taipei Medical University Hospital, Taipei, 110, Taiwan.
authors.forename: Chun-Chao
authors.initials: CC
authors.lastname: Chang
authors.affiliation: School of Public Health, College of Public Health and Nutrition, Taipei Medical University, No. 250 Wu-Hsing Street, Taipei, 11031, Taiwan.
authors.forename: Hung-Yi
authors.initials: HY
authors.lastname: Chiou
authors.affiliation: Department of Food Science, National Taiwan Ocean University, Keelung, 20224, Taiwan. wuchangjer@yahoo.com.tw.|Department of Health and Nutrition Biotechnology, Asia University, Taichung, 41354, Taiwan. wuchangjer@yahoo.com.tw.|Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, 40447, Taiwan. wuchangjer@yahoo.com.tw.
authors.forename: Chang-Jer
authors.initials: CJ
authors.lastname: Wu
mesh_terms: D000818:Animals; D000074322:Antineoplastic Agents, Immunological / Q000008:administration & dosage*; D000068258:Bevacizumab / Q000008:administration & dosage*; D000068818:Cetuximab / Q000008:administration & dosage*; D004195:Disease Models, Animal; D004358:Drug Therapy / Q000379:methods*; D004359:Drug Therapy, Combination / Q000379:methods; D005260:Female; D064593:Heterografts; D006801:Humans; D008807:Mice, Inbred BALB C; D010190:Pancreatic Neoplasms / Q000188:drug therapy*; D016896:Treatment Outcome; D047368:Tumor Burden
chemical_list: D000074322:Antineoplastic Agents, Immunological; D000068258:Bevacizumab; D000068818:Cetuximab
keywords: 5-Fluorouracil; Bevacizumab; Cetuximab; Cisplatin; Gemcitabine; Pancreatic cancer; Xenograft