title: Metabolic phenotyping for monitoring ovarian cancer patients.
abstract: Epithelial ovarian cancer (EOC) is the most deadly of the gynecological cancers. New approaches and better tools for monitoring treatment efficacy and disease progression of EOC are required. In this study, metabolomics using rapid resolution liquid chromatography mass spectrometry was applied to a systematic investigation of metabolic changes in response to advanced EOC, surgery and recurrence. The results revealed considerable metabolic differences between groups. Moreover, 37, 30, and 26 metabolites were identified as potential biomarkers for primary, surgical and recurrent EOC, respectively. Primary EOC was characterized by abnormal lipid metabolism and energy disorders. Oxidative stress and surgical efficacy were clear in the post-operative EOC patients. Recurrent EOC patients showed increased amino acid and lipid metabolism compared with primary EOC patients. After cytoreductive surgery, eight metabolites (e.g. l-kynurenine, retinol, hydroxyphenyllactic acid, 2-octenoic acid) corrected towards levels of the control group, and four (e.g. hydroxyphenyllactic acid, 2-octenoic acid) went back again to primary EOC levels after disease relapse. In conclusion, this study delineated metabolic changes in response to advanced EOC, surgery and recurrence, and identified biomarkers that could facilitate both understanding and monitoring of EOC development and progression.
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Harbin Medical University, Harbin 150086, P.R. China.
authors.forename: Chaofu
authors.initials: C
authors.lastname: Ke
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Harbin Medical University, Harbin 150086, P.R. China.
authors.forename: Ang
authors.initials: A
authors.lastname: Li
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Harbin Medical University, Harbin 150086, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital of Harbin Medical University, and The Key Laboratory of Myocardial Ischemia, Harbin Medical University, Harbin 150086, P.R. China.
authors.forename: Meng
authors.initials: M
authors.lastname: Sun
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Harbin Medical University, Harbin 150086, P.R. China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yang
authors.affiliation: Department of Gynecology Oncology, The Tumor Hospital, Harbin Medical University, Harbin 150086, P.R. China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Harbin Medical University, Harbin 150086, P.R. China.
authors.forename: Jingtao
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Gynecology Oncology, The Tumor Hospital, Harbin Medical University, Harbin 150086, P.R. China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Ge
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Harbin Medical University, Harbin 150086, P.R. China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Harbin Medical University, Harbin 150086, P.R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Harbin Medical University, Harbin 150086, P.R. China.
authors.forename: Junnan
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Harbin Medical University, Harbin 150086, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Gynecology Oncology, The Tumor Hospital, Harbin Medical University, Harbin 150086, P.R. China.
authors.forename: Ge
authors.initials: G
authors.lastname: Lou
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Harbin Medical University, Harbin 150086, P.R. China.
authors.forename: Kang
authors.initials: K
authors.lastname: Li
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D000077216:Carcinoma, Ovarian Epithelial; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D055432:Metabolomics; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000097:blood*; D010051:Ovarian Neoplasms / Q000097:blood*; D010641:Phenotype
chemical_list: D014408:Biomarkers, Tumor