title: Synthesis, Characterization and in Vitro Evaluation of Manganese Ferrite (MnFe2O4) Nanoparticles for Their Biocompatibility with Murine Breast Cancer Cells (4T1).
abstract: Manganese ferrite (MnFe2O4) magnetic nanoparticles were successfully prepared by a sol-gel self-combustion technique using iron nitrate and manganese nitrate, followed by calcination at 150 °C for 24 h. Calcined sample was systematically characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and vibrational sample magnetometry (VSM) in order to identify the crystalline phase, functional group, morphology, particle size, shape and magnetic behavior. It was observed that the resultant spinal ferrites obtained at low temperature exhibit single phase, nanoparticle size and good magnetic behavior. The study results have revealed the existence of a potent dose dependent cytotoxic effect of MnFe2O4 nanoparticles against 4T1 cell lines at varying concentrations with IC50 values of 210, 198 and 171 μg/mL after 24 h, 48 h and 72 h of incubation, respectively. Cells exposed to higher concentrations of nanoparticles showed a progressive increase of apoptotic and necrotic activity. Below 125 μg/mL concentration the nanoparticles were biocompatible with 4T1 cells.
authors.affiliation: Materials Synthesis and Characterization Laboratory (MSCL), Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia. kanagu1980@gmail.com.
authors.forename: Samikannu
authors.initials: S
authors.lastname: Kanagesan
authors.affiliation: Materials Synthesis and Characterization Laboratory (MSCL), Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia. sidekaziz@gmail.com.|Department of Physics, Faculty of Science, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia. sidekaziz@gmail.com.
authors.forename: Sidek Bin Ab
authors.initials: SB
authors.lastname: Aziz
authors.affiliation: Materials Synthesis and Characterization Laboratory (MSCL), Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia. mansorhashim@gmail.com.
authors.forename: Mansor
authors.initials: M
authors.lastname: Hashim
authors.affiliation: Materials Synthesis and Characterization Laboratory (MSCL), Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia. kayzen@gmail.com.
authors.forename: Ismayadi
authors.initials: I
authors.lastname: Ismail
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia. drstamilselvan@gmail.com.
authors.forename: Subramani
authors.initials: S
authors.lastname: Tamilselvan
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia. noorjahan@upm.edu.my.
authors.forename: Noorjahan Banu Binti Mohammed
authors.initials: NB
authors.lastname: Alitheen
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia. swamy.bio@gmail.com.|Department of Crop Science, Universiti Putra Malaysia (UPM), Serdang 43400, Selangor, Malaysia. swamy.bio@gmail.com.
authors.forename: Mallappa Kumara
authors.initials: MK
authors.lastname: Swamy
authors.affiliation: Department of Applied Science and Humanities, Sasi Intitute of Technology and Engineering, Tadepalligudem, West Godavari District 534101, Andhra Pradesh, India. nanopurna@gmail.com.
authors.forename: Bandaru
authors.initials: B
authors.lastname: Purna Chandra Rao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001672:Biocompatible Materials / Q000737:chemistry / Q000627:therapeutic use; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D005260:Female; D005290:Ferric Compounds / Q000008:administration & dosage / Q000737:chemistry* / Q000627:therapeutic use*; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use* / Q000648:ultrastructure; D008325:Mammary Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology; D017895:Manganese Compounds / Q000008:administration & dosage / Q000737:chemistry* / Q000627:therapeutic use*; D008422:Materials Testing; D051379:Mice; D046529:Microscopy, Electron, Transmission; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D017895:Manganese Compounds; C551151:manganese ferrite
keywords: breast cancer; cytotoxicity; magnetization; manganese ferrite; nanoparticles