title: Post-ovulatory aging of mouse oocytes in vivo and in vitro: Effects of caffeine on exocytosis and translocation of cortical granules.
abstract: The developmental potential of post-ovulatory oocytes decreases with aging in vivo and in vitro. In this study, we aimed to investigate the effects of a potent antioxidant caffeine on cortical granules (CGs) distribution in mouse oocytes aging in vivo and in vitro. We found that in vivo administration of 150 mg/kg caffeine caused ovulation of some morphologically abnormal oocytes showing premature exocytosis or congregation of CGs, but significantly decreased abnormal distribution of CGs in oocytes aging for 6 h, 12 h and 18 h in vivo compared to those without caffeine treatment. Unexpectedly, supplementation of oocyte culture medium with 10 mmol/L caffeine accelerated CGs release of oocytes and the normal CG distribution rate dramatically decreased from 6 h in oocytes aging in vitro. It appeared that oocytes showed a high degree of abnormal CG distribution by aging for 18 h, and caffeine might delay oocyte CG exocytosis in vivo, but accelerates CG exocytosis in vitro. Our findings may have implications for improving assisted reproduction technologies.
authors.affiliation: Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong, China.|Institute of Reproductive Sciences, Qingdao Agricultural University, Qingdao, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong, China.|Institute of Reproductive Sciences, Qingdao Agricultural University, Qingdao, China.
authors.forename: Xun-Qiang
authors.initials: XQ
authors.lastname: Yin
authors.affiliation: Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong, China.|Institute of Reproductive Sciences, Qingdao Agricultural University, Qingdao, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ge
authors.affiliation: College of Life Science, Qingdao Agricultural University, Qingdao, China.|Institute of Reproductive Sciences, Qingdao Agricultural University, Qingdao, China.
authors.forename: Gui-Fang
authors.initials: GF
authors.lastname: He
authors.affiliation: Department of Reproductive Medicine; Peking University Shenzhen Hospital, Medical Center of Peking University, Shenzhen, Guangdong, China.
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Qian
authors.affiliation: Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong, China.|Institute of Reproductive Sciences, Qingdao Agricultural University, Qingdao, China.
authors.forename: Jun-Yu
authors.initials: JY
authors.lastname: Ma
authors.affiliation: Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong, China.|Institute of Reproductive Sciences, Qingdao Agricultural University, Qingdao, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
authors.affiliation: Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong, China.|Institute of Reproductive Sciences, Qingdao Agricultural University, Qingdao, China.
authors.forename: Shen
authors.initials: S
authors.lastname: Yin
authors.affiliation: Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong, China. sunqy@ioz.ac.cn.|Institute of Reproductive Sciences, Qingdao Agricultural University, Qingdao, China. sunqy@ioz.ac.cn.|State Key Laboratory of Reproductive Biology; Institute of Zoology, Chinese Academy of Sciences, Beijing, China. sunqy@ioz.ac.cn.
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Sun
mesh_terms: D000818:Animals; D002110:Caffeine / Q000008:administration & dosage / Q000494:pharmacology*; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects* / Q000502:physiology*; D003594:Cytoplasmic Granules / Q000378:metabolism*; D005089:Exocytosis / Q000187:drug effects*; D005260:Female; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism* / Q000473:pathology; D010060:Ovulation / Q000187:drug effects; D027724:Reproductive Techniques, Assisted; D013997:Time Factors
chemical_list: D002110:Caffeine
keywords: aging oocytes; caffeine; cortical granules