title: Fetuin A is down-regulated in rats during heterotopic ossification after Achilles tenotomy.
abstract: We investigated the role of fetuin A during heterotopic ossification (HO) in rats following Achilles tenotomy. We performed a right midpoint Achilles tenotomy on 24 rats. At 5 and 10 h after surgery, we investigated the formation of ectopic bone using X-ray and histological examination. We evaluated the mRNA level of fetuin A using real-time PCR. Presence of fetuin A in the Achilles tendon was assessed by immunohistochemical staining. We also measured the serum concentration of fetuin A using enzyme linked immunosorbent assay (ELISA). The expression of fetuin A was significantly decreased in both the liver and Achilles tendon during HO. ELISA showed a small amount of fetuin A in blood throughout the development of HO. Immunohistochemical staining showed that fetuin A was abundant in the ectopic bone. Fetuin A appears to be involved in the formation of ectopic bone induced by Achilles tenotomy, and a deficiency of fetuin A plays a role in the development of HO.
authors.affiliation: a Department of Orthopedics , Changsha Hospital of Traditional Chinese Medicine (Changsha Eighth Hospital) , Changsha , China.
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.affiliation: b Department of Orthopedics , Southern Medical University Affiliated Fengxian Hospital , Shanghai , China.
authors.forename: F
authors.initials: F
authors.lastname: Xue
authors.affiliation: b Department of Orthopedics , Southern Medical University Affiliated Fengxian Hospital , Shanghai , China.
authors.forename: W Z
authors.initials: WZ
authors.lastname: Shi
authors.affiliation: b Department of Orthopedics , Southern Medical University Affiliated Fengxian Hospital , Shanghai , China.
authors.forename: H J
authors.initials: HJ
authors.lastname: Xiao
mesh_terms: D000125:Achilles Tendon / Q000601:surgery*; D000818:Animals; D004195:Disease Models, Animal; D015536:Down-Regulation; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009999:Ossification, Heterotopic / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D058971:Tenotomy; D060749:alpha-2-HS-Glycoprotein / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D060749:alpha-2-HS-Glycoprotein
keywords: fetuin A; heterotopic ossification; rat