title: Constructing Proteome Reference Map of the Porcine Jejunal Cell Line (IPEC-J2) by Label-Free Mass Spectrometry.
abstract: In this study, the global proteome of the IPEC-J2 cell line was evaluated using ultra-high performance liquid chromatography coupled to a quadrupole Q Exactive™ Orbitrap mass spectrometer. Proteins were isolated from highly confluent IPEC-J2 cells in biological replicates and analyzed by label-free mass spectrometry prior to matching against a porcine genomic dataset. The results identified 1,517 proteins, accounting for 7.35% of all genes in the porcine genome. The highly abundant proteins detected, such as actin, annexin A2, and AHNAK nucleoprotein, are involved in structural integrity, signaling mechanisms, and cellular homeostasis. The high abundance of heat shock proteins indicated their significance in cellular defenses, barrier function, and gut homeostasis. Pathway analysis and annotation using the Kyoto Encyclopedia of Genes and Genomes database resulted in a putative protein network map of the regulation of immunological responses and structural integrity in the cell line. The comprehensive proteome analysis of IPEC-J2 cells provides fundamental insights into overall protein expression and pathway dynamics that might be useful in cell adhesion studies and immunological applications.
authors.affiliation: Department of Animal Resources Science, Dankook, University, Cheonan 31116, Republic of Korea.
authors.forename: Sang Hoon
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Animal Resources Science, Dankook, University, Cheonan 31116, Republic of Korea.
authors.forename: Edward Alain B
authors.initials: EA
authors.lastname: Pajarillo
authors.affiliation: Department of Animal Resources Science, Dankook, University, Cheonan 31116, Republic of Korea.
authors.forename: Marilen P
authors.initials: MP
authors.lastname: Balolong
authors.affiliation: National Instrumentation Center for Environmental Management, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Ji Yoon
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Department of Animal Resources Science, Dankook, University, Cheonan 31116, Republic of Korea.
authors.forename: Dae-Kyung
authors.initials: DK
authors.lastname: Kang
mesh_terms: D000199:Actins / Q000302:isolation & purification; D000818:Animals; D017306:Annexin A2 / Q000302:isolation & purification; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D030562:Databases, Protein; D004847:Epithelial Cells / Q000378:metabolism*; D016678:Genome; D006360:Heat-Shock Proteins / Q000302:isolation & purification; D006706:Homeostasis; D007413:Intestinal Mucosa / Q000276:immunology / Q000378:metabolism*; D007583:Jejunum / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D013058:Mass Spectrometry; D053858:Metabolic Networks and Pathways; D020543:Proteome / Q000032:analysis* / Q000302:isolation & purification; D015398:Signal Transduction; D013552:Swine
chemical_list: D000199:Actins; D017306:Annexin A2; D006360:Heat-Shock Proteins; D020543:Proteome
keywords: IPEC-J2 cell line; Mass spectrometry; Proteome; QExactive™ Orbitrap