title: From Cloning Neural Development Genes to Functional Studies in Mice, 30 Years of Advancements.
abstract: The invention of new mouse molecular genetics techniques, initiated in the 1980s, has repeatedly expanded our ability to tackle exciting developmental biology problems. The brain is the most complex organ, and as such the more sophisticated the molecular genetics technique, the more impact they have on uncovering new insights into how our brain functions. I provide a general time line for the introduction of new techniques over the past 30 years and give examples of new discoveries in the neural development field that emanated from them. I include a look to what the future holds and argue that we are at the dawn of a very exciting age for young scientists interested in studying how the nervous system is constructed and functions with such precision.
authors.affiliation: Developmental Biology Program, Sloan Kettering Institute, New York, USA. Electronic address: joynera@mskcc.org.
authors.forename: Alexandra L
authors.initials: AL
authors.lastname: Joyner
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D050437:Genes, Developmental / Q000235:genetics*; D051379:Mice; D009420:Nervous System / Q000254:growth & development*; D055495:Neurogenesis / Q000502:physiology*; D013997:Time Factors
keywords: CRISPER/Cas9; Cerebellum; Clonal analysis; Cre/loxP; Embryonic stem cells; Gene targeting; Genetic fate mapping; Genome engineering; Neocortex; Neurogenesis