title: Demonstration of Herpes Simplex Virus, Cytomegalovirus, and Epstein-Barr Virus in Colorectal Cancer.
abstract: BACKGROUND
  The present study sought to investigate molecular evidence for association between the presence of herpes simplex virus (HSV), cytomegalovirus (CMV), and Epstein-Barr virus (EBV) in CRC and colorectal polyp by using the PCR method in Iran.
  
  
  METHODS
  In this analytical case-control study, we selected 15 patients with CRC, 20 patients with colorectal polyp, and 35 patients without malignancy as controls. After DNA extraction, PCR was used to determine HSV, CMV, and EBV genome by specific primers. Statistical analysis was performed using χ2 tests.
  
  
  RESULTS
  Our findings demonstrated that there is no direct molecular evidence to support the association between HSV, CMV, and EBV and human colorectal malignancies.
  
  
  CONCLUSIONS
  The results from this study do not exclude a possible oncogenic role of these viruses in neoplastic development of colon cells.
authors.affiliation: Department of Microbiology, Tonekabon Branch, Islamic Azad University, Mazandaran, Iran.
authors.forename: Sahar
authors.initials: S
authors.lastname: Mehrabani-Khasraghi
authors.affiliation: Department of Medicine, Tonekabon Branch, Islamic Azad University, Mazandaran, Iran.
authors.forename: Mitra
authors.initials: M
authors.lastname: Ameli
authors.affiliation: Gastroenterology and Hepatology Research Center, Karaj University of Medical Sciences and Health Services, Alborz, Iran.
authors.forename: Farzad
authors.initials: F
authors.lastname: Khalily
mesh_terms: D016022:Case-Control Studies; D003111:Colonic Polyps / Q000821:virology*; D015179:Colorectal Neoplasms / Q000821:virology*; D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D016133:Polymerase Chain Reaction; D018139:Simplexvirus / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D017931:DNA Primers; D004279:DNA, Viral
keywords: Colorectal cancer; Cytomegalovirus; Epstein-Bar virus; Herpes simplex virus; PCR