title: Influence of fat intake and BMI on the association of rs1799983 NOS3 polymorphism with blood pressure levels in an Iberian population.
abstract: OBJECTIVE
  There is controversy about the effect of the rs1799983 nitric oxide synthase (NOS3) genetic variant on hypertension and blood pressure (BP) levels. The aims of the current study were to examine whether rs1799983 affects BP levels and to identify potential interactions between this polymorphism and other non-genetic risk factors.
  
  
  METHODS
  A total of 705 subjects were examined for anthropometric and body composition measurements, BP, dietary habits and physical activity. Oral epithelial cells were collected for the identification of rs1799983 using Luminex®100/200TM System.
  
  
  RESULTS
  After adjusted for covariates, TT genotype showed a 2.30-fold higher predisposition of hypertension than GG genotype subjects. According to BP levels, for each risk allele diastolic blood pressure (DBP) increased in 1.99 mmHg. Significant interactions between rs1799983 and saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) were found. Moreover, an interaction with body weight status was observed. Among overweight individuals, T allele carriers showed higher DBP than GG genotype.
  
  
  CONCLUSIONS
  The present study evidenced that rs1799983 NOS3 polymorphism could be associated with hypertension and DBP among Southern Europeans, being this association influenced by dietary fat (SFA and MUFA) and body mass index.
authors.affiliation: Department of Nutrition, Food Sciences and Physiology, University of Navarra, Irunlarrea, 1, 31008, Pamplona, Navarra, Spain.|Centre for Nutrition Research, University of Navarra, Pamplona, Spain.
authors.forename: Leticia
authors.initials: L
authors.lastname: Goni
authors.affiliation: Department of Nutrition, Food Sciences and Physiology, University of Navarra, Irunlarrea, 1, 31008, Pamplona, Navarra, Spain.|Centre for Nutrition Research, University of Navarra, Pamplona, Spain.|CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), Instituto de Salud Carlos III, Madrid, Spain.|Instituto de Investigación Sanitaria de Navarra (IdiSNA), Pamplona, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Cuervo
authors.affiliation: Department of Nutrition, Food Sciences and Physiology, University of Navarra, Irunlarrea, 1, 31008, Pamplona, Navarra, Spain.|Centre for Nutrition Research, University of Navarra, Pamplona, Spain.|CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Fermín I
authors.initials: FI
authors.lastname: Milagro
authors.affiliation: Department of Nutrition, Food Sciences and Physiology, University of Navarra, Irunlarrea, 1, 31008, Pamplona, Navarra, Spain. jalfmtz@unav.es.|Centre for Nutrition Research, University of Navarra, Pamplona, Spain. jalfmtz@unav.es.|CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), Instituto de Salud Carlos III, Madrid, Spain. jalfmtz@unav.es.|Instituto de Investigación Sanitaria de Navarra (IdiSNA), Pamplona, Spain. jalfmtz@unav.es.
authors.forename: J Alfredo
authors.initials: JA
authors.lastname: Martínez
mesh_terms: D000328:Adult; D000483:Alleles; D001794:Blood Pressure / Q000235:genetics*; D001795:Blood Pressure Determination; D015992:Body Mass Index; D003430:Cross-Sectional Studies; D004041:Dietary Fats / Q000008:administration & dosage; D005227:Fatty Acids / Q000008:administration & dosage; D005229:Fatty Acids, Monounsaturated / Q000008:administration & dosage; D005260:Female; D059647:Gene-Environment Interaction; D005838:Genotype; D060005:Genotyping Techniques; D006801:Humans; D006973:Hypertension / Q000453:epidemiology* / Q000235:genetics*; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D010865:Pilot Projects; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D013030:Spain / Q000453:epidemiology
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III
keywords: Blood pressure; Hypertension; Monounsaturated fatty acids; NOS3; Obesity; Saturated fatty acids