title: Exploring the glycan interaction in vivo: Future prospects of neo-glycoproteins for diagnostics.
abstract: Herein the biodistributions and in vivo kinetics of chemically prepared neoglycoproteins are reviewed. Chemical methods can be used to conjugate various mono- and oligosaccharides onto a protein surface. The kinetics and organ-specific accumulation profiles of these glycoconjugates, which are introduced through intravenous injections, have been analyzed using conventional dissection studies as well as noninvasive methods such as single photon emission computed tomography, positron emission tomography and fluorescence imaging. These studies suggest that glycan-dependent protein distribution kinetics may be useful for pharmacological and diagnostic applications.
authors.affiliation: Biofunctional Synthetic Chemistry Laboratory, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ogura
authors.affiliation: Biofunctional Chemistry Laboratory, A. Butlerov Institute of Chemistry, Kazan Federal University, 18 Kremlyovskaya street, Kazan 420008, Russia.
authors.forename: Almira
authors.initials: A
authors.lastname: Kurbangalieva
authors.affiliation: Biofunctional Synthetic Chemistry Laboratory, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan Biofunctional Chemistry Laboratory, A. Butlerov Institute of Chemistry, Kazan Federal University, 18 Kremlyovskaya street, Kazan 420008, Russia JST PRESTO, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan kotzenori@riken.jp.
authors.forename: Katsunori
authors.initials: K
authors.lastname: Tanaka
mesh_terms: D000586:Amination; D000818:Animals; D002240:Carbohydrate Sequence; D003545:Cysteine / Q000737:chemistry; D006001:Glycoconjugates / Q000737:chemistry* / Q000378:metabolism / Q000493:pharmacokinetics; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism / Q000493:pharmacokinetics; D006031:Glycosylation; D007275:Injections, Intravenous; D008239:Lysine / Q000737:chemistry; D051379:Mice; D009005:Monosaccharides / Q000737:chemistry* / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism; D061848:Optical Imaging / Q000379:methods; D010084:Oxidation-Reduction; D000072078:Positron Emission Tomography Computed Tomography / Q000379:methods; D000072098:Single Photon Emission Computed Tomography Computed Tomography / Q000379:methods
chemical_list: D006001:Glycoconjugates; D006023:Glycoproteins; D009005:Monosaccharides; D009844:Oligosaccharides; D008239:Lysine; D003545:Cysteine
keywords: N-glycans; albumin; in vivo imaging; neoglycoprotein; tumor targeting