title: Hepatoma-Targeted Radionuclide Immune Albumin Nanospheres: (131)I-antiAFPMcAb-GCV-BSA-NPs.
abstract: An effective strategy has been developed for synthesis of radionuclide immune albumin nanospheres ((131)I-antiAFPMcAb-GCV-BSA-NPs). In vitro as well as in vivo targeting of (131)I-antiAFPMcAb-GCV-BSA-NPs to AFP-positive hepatoma was examined. In cultured HepG2 cells, the uptake and retention rates of (131)I-antiAFPMcAb-GCV-BSA-NPs were remarkably higher than those of (131)I alone. As well, the uptake rate and retention ratios of (131)I-antiAFPMcAb-GCV-BSA-NPs in AFP-positive HepG2 cells were also significantly higher than those in AFP-negative HEK293 cells. Compared to (131)I alone, (131)I-antiAFPMcAb-GCV-BSA-NPs were much more easily taken in and retained by hepatoma tissue, with a much higher T/NT. Due to good drug-loading, high encapsulation ratio, and highly selective affinity for AFP-positive tumors, the (131)I-antiAFPMcAb-GCV-BSA-NPs are promising for further effective radiation-gene therapy of hepatoma.
authors.affiliation: Clinical Medical Institute, Taizhou People's Hospital Affiliated to Nantong University, Taizhou, Jiangsu 225300, China; Medical School, Southeast University, Nanjing, Jiangsu 210009, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Lin
authors.affiliation: Clinical Medical Institute, Taizhou People's Hospital Affiliated to Nantong University, Taizhou, Jiangsu 225300, China.
authors.forename: Junxing
authors.initials: J
authors.lastname: Huang
authors.affiliation: Medical School, Southeast University, Nanjing, Jiangsu 210009, China; Jiangsu Key Laboratory for Biomaterials and Devices, Nanjing, Jiangsu 210009, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Advanced Catalytic Material and Technology, Changzhou University, Changzhou, Jiangsu 213000, China.
authors.forename: Xingmao
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Medical School, Southeast University, Nanjing, Jiangsu 210009, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Clinical Medical Institute, Taizhou People's Hospital Affiliated to Nantong University, Taizhou, Jiangsu 225300, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yu
authors.affiliation: Clinical Medical Institute, Taizhou People's Hospital Affiliated to Nantong University, Taizhou, Jiangsu 225300, China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Clinical Medical Institute, Taizhou People's Hospital Affiliated to Nantong University, Taizhou, Jiangsu 225300, China.
authors.forename: Yujuan
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Clinical Medical Institute, Taizhou People's Hospital Affiliated to Nantong University, Taizhou, Jiangsu 225300, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Guo
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000276:immunology / Q000627:therapeutic use*; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D059248:Chemoradiotherapy / Q000379:methods; D016503:Drug Delivery Systems / Q000379:methods; D005260:Female; D015774:Ganciclovir / Q000008:administration & dosage / Q000627:therapeutic use*; D015316:Genetic Therapy / Q000379:methods; D057809:HEK293 Cells; D056945:Hep G2 Cells; D006801:Humans; D007457:Iodine Radioisotopes / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use*; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D046529:Microscopy, Electron, Transmission; D054118:Nanospheres / Q000008:administration & dosage / Q000737:chemistry / Q000648:ultrastructure; D012710:Serum Albumin, Bovine / Q000737:chemistry; D014018:Tissue Distribution; D023041:Xenograft Model Antitumor Assays; D000509:alpha-Fetoproteins / Q000235:genetics / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes; D000509:alpha-Fetoproteins; D012710:Serum Albumin, Bovine; D015774:Ganciclovir