title: Total peroxiredoxin expression is associated with survival in patients with follicular lymphoma.
abstract: Redox state-regulating enzymes may have roles in chemoresistance and also in lymphomagenesis, but there have been only a limited number of studies on this topic in lymphomas. Our aim was to assess expression of the redox state-regulating enzymes peroxiredoxins (Prxs) I-VI and thioredoxin (Trx) and the oxidative stress marker nitrotyrosine in follicular lymphomas (FLs). We immunohistochemically assessed Prxs I-VI, Trx and nitrotyrosine in a cohort of 76 histologically confirmed, untreated FLs. We also studied the localisation of Prxs I, II, III, V and VI by means of immunoelectron microscopy (IEM). Immunohistochemistry results were correlated with disease-specific survival (DSS), progression-free survival (PFS), overall survival (OS) and clinical prognostic factors. When all Prx expression intensities were grouped as a single variable, we discovered that high total Prx intensity correlated with favourable DSS (p = 0.024) and OS (p = 0.035) but not with PFS. No deaths due to lymphoma were recorded amongst patients with high total Prx expression during the median follow-up period of 7.6 years. IEM results were in line with earlier ones demonstrating wide subcellular localisation of Prx isoenzymes. In conclusion, our results demonstrate an association between high total Prx expression and prolonged survival and suggest that Prxs may have a protective role in FL that cannot be compensated by other antioxidant mechanisms.
authors.affiliation: Department of Oncology and Radiotherapy, University of Oulu and Medical Research Center, Oulu University Hospital, P.O. Box 22, 90029, Oulu, Finland. pekka.peroja@student.oulu.fi.
authors.forename: Pekka
authors.initials: P
authors.lastname: Peroja
authors.affiliation: Department of Pathology, University of Oulu and Medical Research Center, Oulu, Finland.
authors.forename: Kirsi-Maria
authors.initials: KM
authors.lastname: Haapasaari
authors.affiliation: Cancer Center, Department of Oncology and Genome Scale Research Program, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
authors.forename: Susanna
authors.initials: S
authors.lastname: Mannisto
authors.affiliation: Biocenter Oulu, University of Oulu, Oulu, Finland.
authors.forename: Ilkka
authors.initials: I
authors.lastname: Miinalainen
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Oulu University Hospital and University of Oulu, Helsinki, Finland.
authors.forename: Petri
authors.initials: P
authors.lastname: Koivunen
authors.affiliation: Cancer Center, Department of Oncology and Genome Scale Research Program, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
authors.forename: Sirpa
authors.initials: S
authors.lastname: Leppä
authors.affiliation: HUSLAB, Department of Pathology, Helsinki University Hospital, Helsinki, Finland.
authors.forename: Marja-Liisa
authors.initials: ML
authors.lastname: Karjalainen-Lindsberg
authors.affiliation: Department of Oncology and Radiotherapy, University of Oulu and Medical Research Center, Oulu University Hospital, P.O. Box 22, 90029, Oulu, Finland.
authors.forename: Milla Elvi Linnea
authors.initials: ME
authors.lastname: Kuusisto
authors.affiliation: Department of Oncology and Radiotherapy, University of Oulu and Medical Research Center, Oulu University Hospital, P.O. Box 22, 90029, Oulu, Finland.
authors.forename: Taina
authors.initials: T
authors.lastname: Turpeenniemi-Hujanen
authors.affiliation: Department of Oncology and Radiotherapy, University of Oulu and Medical Research Center, Oulu University Hospital, P.O. Box 22, 90029, Oulu, Finland.
authors.forename: Outi
authors.initials: O
authors.lastname: Kuittinen
authors.affiliation: Department of Oncology and Radiotherapy, University of Oulu and Medical Research Center, Oulu University Hospital, P.O. Box 22, 90029, Oulu, Finland.
authors.forename: Peeter
authors.initials: P
authors.lastname: Karihtala
mesh_terms: D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000378:metabolism*; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008224:Lymphoma, Follicular / Q000378:metabolism* / Q000401:mortality* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology; D054464:Peroxiredoxins / Q000378:metabolism*; D013879:Thioredoxins / Q000378:metabolism
chemical_list: D000975:Antioxidants; D013879:Thioredoxins; D054464:Peroxiredoxins
keywords: Follicular lymphoma; Immunohistochemistry; Oxidative stress; Peroxiredoxins; Prognostic factors; Thioredoxin