title: Depletion of mitochondrial enzyme system in liver, lung, brain, stomach and kidney induced by benzo(a)pyrene.
abstract: Mitochondrial dysfunction has recently received considerable attention as it plays an important role in adult human pathology caused by various drugs, endogenous agents and environmental agents. Benzo(a)pyrene (BaP), is a ubiquitous environmental contaminant mainly derived from anthropogenic activity during incomplete combustion of organic materials from various sources. The present study aimed to evaluate the effects of benzo(a)pyrene (BaP) on mitochondrial enzymes in the multiple organs including liver, lung, brain, stomach and kidney. ICR mice were exposed to different doses of BaP (2.5, 5 and 10mg/kg body weight) through oral gavage and intraperitoneal injection treatment for 13 weeks consecutively. The induced mitochondrial damage in the examined organs was assayed in terms of significant increase in lipid peroxidation (LPO) and prominent decrease in antioxidant enzymes. Non enzymatic antioxidants and Krebs cycle's enzymes were also significantly decreased in mitochondria. Additionally, BaP induced the body growth retardation and decrease in relative liver weight, increase in relative lung, stomach, kidney and brain weights, and this was further certified through histopathological lesions. Liver and lungs were more prominently damaged by BaP. The mitochondrial depletion increased in BaP dose-dependent manner.
authors.affiliation: Department of Biological Science and Engineering, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, 710049, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Ji
authors.affiliation: School of Materials and Chemical Engineering, Xi'an Technological University, Xi'an 710032, China.
authors.forename: Yongfei
authors.initials: Y
authors.lastname: Li
authors.affiliation: Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Jianlong
authors.initials: J
authors.lastname: He
authors.affiliation: Institute of Basic Medical Sciences, Khyber Medical University, Peshawar, Pakistan.
authors.forename: Walayat
authors.initials: W
authors.lastname: Shah
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Biopharmaceutics School of Pharmacy, Fourth Military Medical University, Xi'an, Shaanxi 710032, China.
authors.forename: Xiaochang
authors.initials: X
authors.lastname: Xue
authors.affiliation: Department of Neurology, Xijing Hospital, Fourth Military Medical University, 17 Changle West Road, Shaanxi, Xi'an 710032, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Feng
authors.affiliation: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Huqin
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Biological Science and Engineering, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, 710049, China. Electronic address: gaomeili@mail.xjtu.edu.cn.
authors.forename: Meili
authors.initials: M
authors.lastname: Gao
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D015386:Hazardous Substances / Q000633:toxicity*; D006801:Humans; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D051379:Mice; D008813:Mice, Inbred ICR; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology*; D013270:Stomach / Q000201:enzymology; D018675:Toxicity Tests
chemical_list: D015386:Hazardous Substances; D001564:Benzo(a)pyrene
keywords: Benzo(a)pyrene; Depletion of mitochondrial enzymes; Histopathological lesion; Multiple organs