title: Relation between iron metabolism and antioxidants enzymes and δ-ALA-D activity in rats experimentally infected by Fasciola hepatica.
abstract: The aim of this study was to evaluate the iron metabolism in serum, as well as antioxidant enzymes, in addition to the Delta-Aminolevulinic Acid Dehydratase (δ-ALA-D) activity in the liver of rats experimentally infected by Fasciola hepatica. Thirty male adult rats (Wistar) specific pathogen free were divided into four groups: two uninfected group (CTRL 1 and CTRL 2) with five animals each and two infected groups (INF 1 and INF 2) with 10 animals each. Infection was performed orally with 20 metacercariae at day 1. On day 15 (CTRL 1 and INF 1 groups) and 87 PI (CTRL 2 and INF 2 groups) blood and bone marrow were collected and the animals were subsequently euthanized for liver sampling. Blood was allocated in tubes without anticoagulant for serum acquisition to measure iron, transferrin and unsaturated iron binding capacity (UIBC). δ-ALA-D, superoxide dismutase (SOD), and catalase (CAT) activities were measured in the liver. A decrease in iron, transferrin and UIBC levels was observed in all infected animals compared to control groups (P < 0.05). Furthermore, iron accumulation was observed in bone marrow of infected mice. Infected animals showed an increase in δ-ALA-D activity at 87 post-infection (PI) (INF 2) as well as in SOD activity at days 15 (INF 1) and 87 PI (INF 2). On the other hand, CAT activity was reduced in rats infected by F. hepatica during acute and chronic phase of fasciolosis (INF 1 and INF 2 groups), when moderate (acute) and severe necrosis in the liver histopathology were observed. These results may suggest that oxidative damage to tissues along with antioxidant mechanisms might have taken part in fasciolosis pathogenesis and are also involved in iron deficiency associated to changes in δ-ALA-D activity during chronic phase of disease.
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Nathieli B
authors.initials: NB
authors.lastname: Bottari
authors.affiliation: Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil. Electronic address: ricardo.mendes@ifc-concordia.edu.br.
authors.forename: Ricardo E
authors.initials: RE
authors.lastname: Mendes
authors.affiliation: Department of Microbiology and Parasitology, UFSM, Santa Maria, RS, Brazil.
authors.forename: Matheus D
authors.initials: MD
authors.lastname: Baldissera
authors.affiliation: Department of Clinical and Toxicological Analysis, UFSM, Santa Maria, RS, Brazil.
authors.forename: Guilherme V
authors.initials: GV
authors.lastname: Bochi
authors.affiliation: Department of Clinical and Toxicological Analysis, UFSM, Santa Maria, RS, Brazil.
authors.forename: Rafael N
authors.initials: RN
authors.lastname: Moresco
authors.affiliation: Department of Large Animals, UFSM, Santa Maria, RS, Brazil.
authors.forename: Marta L R
authors.initials: ML
authors.lastname: Leal
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Vera M
authors.initials: VM
authors.lastname: Morsch
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Maria R C
authors.initials: MR
authors.lastname: Schetinger
authors.affiliation: Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Christ
authors.affiliation: Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil.
authors.forename: Larissa
authors.initials: L
authors.lastname: Gheller
authors.affiliation: Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil.
authors.forename: Éder J
authors.initials: ÉJ
authors.lastname: Marques
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil; Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil. Electronic address: aleksandro_ss@yahoo.com.br.
authors.forename: Aleksandro S
authors.initials: AS
authors.lastname: Da Silva
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001853:Bone Marrow / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D005210:Fasciola hepatica / Q000302:isolation & purification; D005211:Fascioliasis / Q000201:enzymology / Q000378:metabolism*; D005243:Feces / Q000469:parasitology; D007501:Iron / Q000097:blood / Q000378:metabolism*; D008099:Liver / Q000201:enzymology / Q000469:parasitology; D008297:Male; D010100:Oxygen / Q000378:metabolism; D000623:Porphobilinogen Synthase / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D012756:Sheep; D012908:Snails; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D007501:Iron; D002374:Catalase; D013482:Superoxide Dismutase; D000623:Porphobilinogen Synthase; D010100:Oxygen
keywords: Antioxidant; Delta-Aminolevulinic Acid Dehydratase; Fasciolosis; Iron