title: Preparation of magnetic glycoprotein-imprinted nanoparticles with dendritic polyethyleneimine as a monomer for the specific recognition of ovalbumin from egg white.
abstract: Glycoproteins are crucial in massive physiological events and clinical application. It is necessary to prepare new materials to isolate the specific glycoprotein. New and simple core-shell molecularly imprinted polymers were prepared by surface imprinting. The polymers are synthesized with magnetic nanoparticles as the core, water-soluble dendritic polyethyleneimine as the monomer and the ovalbumin as the template. The prepared imprinted polymers showed thin imprinted shell, biocompatibility and superparamagnetic properties. The resultant materials exhibited fast kinetics, high adsorption capacity, perfect selectivity and reusability. More important, they can absorb the template glycoprotein from the neutral solution and successfully be applied to recognize the ovalbumin from egg white, which means that they can provide an alternate method to isolate glycoprotein from bodily fluids.
authors.affiliation: Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an, China.|College of Pharmacy, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an, China.|College of Pharmacy, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Yuhai
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an, China.|College of Pharmacy, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Hao
authors.affiliation: Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an, China.|College of Pharmacy, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Gaiyan
authors.initials: G
authors.lastname: He
authors.affiliation: State key laboratory of multiphase flow in power engineering, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Bianbian
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Ruixia
authors.initials: R
authors.lastname: Gao
authors.affiliation: College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
mesh_terms: D004529:Egg White / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D054802:Molecular Imprinting; D010047:Ovalbumin / Q000032:analysis*; D011094:Polyethyleneimine / Q000737:chemistry*
chemical_list: D006023:Glycoproteins; D058185:Magnetite Nanoparticles; D011094:Polyethyleneimine; D010047:Ovalbumin
keywords: Dendritic polyethyleneimine; Glycoproteins; Magnetic separation; Surface imprinting