title: Kinesin-1 inhibits the aggregation of amyloid-β peptide as detected by fluorescence cross-correlation spectroscopy.
abstract: Although the exact etiology and pathogenesis of Alzheimer's disease (AD) are still unclear, amyloid-β (Aβ) generated by the proteolytic processing of amyloid-β precursor protein (APP) aggregate to form toxic amyloid species. Kinesin-1 is the first identified ATP-dependent axonal transport motor protein that has been proven to affect Aβ generation and deposition. In this paper, we applied dual-color fluorescence cross-correlation spectroscopy (DC-FCCS) to investigate the direct interaction of Aβ with kinesin-1 at the single-molecule fluorescence level in vitro. The results showed that two kinds of enhanced green fluorescent protein (EGFP)-tagged kinesin light-chain subunits of kinesin-1(KLCs), KLC-E and E-KLC inhibited the aggregation of Aβ over a period of time, providing additional insight into the mechanism of axonal transport deficits in AD.
authors.affiliation: College of Life Sciences and Bioengineering, Beijing Jiaotong University, China.
authors.forename: Yanpeng
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: College of Life Sciences and Bioengineering, Beijing Jiaotong University, China.
authors.forename: Shijun
authors.initials: S
authors.lastname: Tian
authors.affiliation: College of Life Sciences and Bioengineering, Beijing Jiaotong University, China.
authors.forename: Xianglei
authors.initials: X
authors.lastname: Peng
authors.affiliation: Beijing National Laboratory for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China.
authors.forename: Jingfa
authors.initials: J
authors.lastname: Yang
authors.affiliation: College of Life Sciences and Bioengineering, Beijing Jiaotong University, China.
authors.forename: Yuanhui
authors.initials: Y
authors.lastname: Fu
authors.affiliation: College of Life Sciences and Bioengineering, Beijing Jiaotong University, China.
authors.forename: Yueying
authors.initials: Y
authors.lastname: Jiao
authors.affiliation: Beijing National Laboratory for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhao
authors.affiliation: College of Life Sciences and Bioengineering, Beijing Jiaotong University, China.
authors.forename: Jinsheng
authors.initials: J
authors.lastname: He
authors.affiliation: College of Life Sciences and Bioengineering, Beijing Jiaotong University, China.|Institute for Viral Disease Control and Prevention, Chinese Centre for Disease Control and Prevention, Beijing, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Hong
mesh_terms: D000465:Algorithms; D019943:Amino Acid Substitution; D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001057:Apolipoproteins E / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D005456:Fluorescent Dyes / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D016547:Kinesins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D009154:Mutation; D010446:Peptide Fragments / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D066263:Protein Aggregation, Pathological / Q000378:metabolism / Q000517:prevention & control*; D054730:Protein Interaction Domains and Motifs; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence
chemical_list: D016229:Amyloid beta-Peptides; D001057:Apolipoproteins E; D005456:Fluorescent Dyes; C488101:KIF5B protein, human; D010446:Peptide Fragments; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins; C075222:amyloid beta-protein (1-42); C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D016547:Kinesins
keywords: Alzheimer's disease; amyloid β; fluorescence cross-correlation spectroscopy; kinesin-1