title: Application of ionic liquids for elution of bioactive flavonoid glycosides from lime fruit by miniaturized matrix solid-phase dispersion.
abstract: In this work, two flavonoid glycosides (neohesperidin and naringin) in lime fruit were effectively extracted by miniaturized matrix solid phase dispersion (MSPD), followed by ultra-performance liquid chromatography-ultraviolet detection. The best results were obtained using Florisil (150mg) as the sorbent and 1-butyl-3-methylimidazolium tetrafluoroborate (0.4mL, 250mM) as the elution solvent. This work represents the first attempt of using ionic liquids as a green eluent for extraction of the investigated compounds in miniaturized MSPD. Compared with the conventional methods, the proposed method is advantageous due to improved enrichment factor and reduced reagent consumption. A good linearity was observed with r(2) values (>0.998). Meanwhile, the method gave acceptable recoveries (90.16-96.47%) for the determination of flavonoids in plant samples. The limits of detection of the two analytes ranged between 4.08 and 5.04μg/g. The results showed that the optimized method has a great potential for sample preparation in routine analysis of complex plant samples.
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Xu
authors.affiliation: Department of Surgery, University of Michigan Medical Center, Ann Arbor, MI 48109, United States.
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
authors.affiliation: Hangzhou Red Cross Hospital, Department of Traditional Chinese Medicine, Hangzhou 310003, China.
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Ye
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China. Electronic address: caojun91@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Wan
authors.initials: W
authors.lastname: Cao
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Shuai-Shuai
authors.initials: SS
authors.lastname: Hu
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Li-Qing
authors.initials: LQ
authors.lastname: Peng
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D032082:Citrus aurantiifolia / Q000737:chemistry*; D044950:Flavanones / Q000032:analysis*; D005638:Fruit / Q000737:chemistry; D006569:Hesperidin / Q000031:analogs & derivatives* / Q000032:analysis; D007093:Imidazoles / Q000737:chemistry; D052578:Ionic Liquids / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods; D012997:Solvents / Q000737:chemistry
chemical_list: C419324:1-butyl-3-methylimidazolium tetrafluoroborate; D044950:Flavanones; D007093:Imidazoles; D052578:Ionic Liquids; D012997:Solvents; D006569:Hesperidin; C005274:naringin; C546526:neohesperidin
keywords: Flavonoid glycosides; Ionic liquids; Lime fruit; Miniaturized matrix solid-phase dispersion; Ultra-performance liquid chromatography