title: Genotyping of Clostridium perfringens isolated from broiler meat in northeastern of Iran.
abstract: Clostridium perfringens (C. perfringens) is an important cause of bacterial food poisoning worldwide. The disease is caused by C. perfringens enterotoxin (CPE) encoded by cpe gene. The aim of this research was to identify the different types of C. perfringens and the presence of cpe gene in isolated bacteria from broilers' meat marketed in retail meat shops of Mashhad city in Northeastern of Iran. After isolation of C. perfringens using conventional culture method and confirmation by specific 16S rDNA gene, a multiplex polymerase chain reaction assay with specific primers, were performed for toxin typing of isolates. Clostridium perfringens was isolated from 31 broilers' meat samples (15.50%) out of 200 samples and for toxin typing the results showed 9 isolates as type A (29.03%) and 22 isolates as type C (70.96%). In this study, cpe-positive C. perfringens were detected in eight isolates of type C (25.00%). Our results indicated that C. perfringens type C is the most common type in broiler chicken carcasses.
authors.affiliation: Graduate Student, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran;
authors.forename: Asma
authors.initials: A
authors.lastname: Afshari
authors.affiliation: Department of Food Hygiene, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran;
authors.forename: Abdollah
authors.initials: A
authors.lastname: Jamshidi
authors.affiliation: Department of Clinical Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran;
authors.forename: Jamshid
authors.initials: J
authors.lastname: Razmyar
authors.affiliation: Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Mehrnaz
authors.initials: M
authors.lastname: Rad
keywords: Clostridium perfringens; Multiplex PCR; cpe gene