title: Effects of C-xylopyranoside derivative on epithelial regeneration in an in vitro 3D oral mucosa model.
abstract: Identifying substandard tissue-engineered oral mucosa grafts with a poor epithelium before clinical use is critical to ensure quality assurance/control in regenerative medicine, leading to success of grafting. This study investigated the effects of one of the C-xylopyranoside derivatives, β-D-xylopyranoside-n-propane-2-one (XPP), on oral epithelial regeneration. Using a three-dimensional oral mucosa model, we analyzed changes of the epithelial structure, glycosaminoglycan (GAG) synthesis, the expression levels of basement membrane zone markers, and substrates of Akt/mTOR signaling. Compared with the control, 2 mM XPP treatment increased the mean and minimal epithelial thickness, and reduced the variation of epithelial thickness. It also stimulated expressions of decorin and syndecan-1 with change of GAG amount and/or composition, and enhanced the expressions of integrin α6, CD44, and Akt/mTOR signaling substrates. These findings suggest that XPP supplementation contributes to consistent epithelial regeneration. Moreover, upregulation of those markers may play a role in increasing the quality of the oral mucosal epithelium.
authors.affiliation: a Division of Oral and Maxillofacial Surgery, Graduate School of Medical and Dental Sciences , Niigata University , Niigata , Japan.|b Division of Oral Anatomy, Graduate School of Medical and Dental Sciences , Niigata University , Niigata , Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Uenoyama
authors.affiliation: c Department of Glycotechnology, Center for Advanced Medical Research , Hirosaki University, Graduate School of Medicine , Hirosaski , Japan.
authors.forename: Ikuko
authors.initials: I
authors.lastname: Kakizaki
authors.affiliation: d Division of Dental Educational Research Development, Graduate School of Medical and Dental Sciences , Niigata University , Niigata , Japan.
authors.forename: Aki
authors.initials: A
authors.lastname: Shiomi
authors.affiliation: b Division of Oral Anatomy, Graduate School of Medical and Dental Sciences , Niigata University , Niigata , Japan.
authors.forename: Naoaki
authors.initials: N
authors.lastname: Saito
authors.affiliation: a Division of Oral and Maxillofacial Surgery, Graduate School of Medical and Dental Sciences , Niigata University , Niigata , Japan.|e Division of Biomimetics, Graduate School of Medical and Dental Sciences , Niigata University , Niigata, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Hara
authors.affiliation: a Division of Oral and Maxillofacial Surgery, Graduate School of Medical and Dental Sciences , Niigata University , Niigata , Japan.
authors.forename: Taro
authors.initials: T
authors.lastname: Saito
authors.affiliation: a Division of Oral and Maxillofacial Surgery, Graduate School of Medical and Dental Sciences , Niigata University , Niigata , Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Ohnuki
authors.affiliation: e Division of Biomimetics, Graduate School of Medical and Dental Sciences , Niigata University , Niigata, Japan.|f Research Center for Advanced Oral Science, Graduate School of Medical and Dental Sciences , Niigata University , Niigata , Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kato
authors.affiliation: a Division of Oral and Maxillofacial Surgery, Graduate School of Medical and Dental Sciences , Niigata University , Niigata , Japan.
authors.forename: Ritsuo
authors.initials: R
authors.lastname: Takagi
authors.affiliation: b Division of Oral Anatomy, Graduate School of Medical and Dental Sciences , Niigata University , Niigata , Japan.|f Research Center for Advanced Oral Science, Graduate School of Medical and Dental Sciences , Niigata University , Niigata , Japan.
authors.forename: Takeyasu
authors.initials: T
authors.lastname: Maeda
authors.affiliation: e Division of Biomimetics, Graduate School of Medical and Dental Sciences , Niigata University , Niigata, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Izumi
mesh_terms: D001485:Basement Membrane / Q000187:drug effects / Q000378:metabolism; D058575:Decorin / Q000235:genetics / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation; D006025:Glycosaminoglycans / Q000819:agonists / Q000096:biosynthesis*; D006027:Glycosides / Q000494:pharmacology*; D006801:Humans; D018960:Hyaluronan Receptors; D039503:Integrin alpha6 / Q000235:genetics / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008954:Models, Biological; D009061:Mouth Mucosa / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D061251:Primary Cell Culture; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism; D012038:Regeneration / Q000187:drug effects* / Q000235:genetics; D015398:Signal Transduction; D053668:Syndecan-1 / Q000235:genetics / Q000378:metabolism; D058570:TOR Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D023822:Tissue Engineering
chemical_list: C473647:CD44S antigen; C546878:DCN protein, human; D058575:Decorin; D006025:Glycosaminoglycans; D006027:Glycosides; D018960:Hyaluronan Receptors; D039503:Integrin alpha6; C508241:SDC1 protein, human; D053668:Syndecan-1; C546842:MTOR protein, human; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases
keywords: epithelium; glycosaminoglycan; in vitro model; oral mucosa; regenerative medicine