title: Down-regulation of tumor endothelial marker 8 suppresses cell proliferation mediated by ERK1/2 activity.
abstract: Tumor endothelial marker 8 (TEM8) was recently suggested as a putative anti-tumor target in several types of human cancer based on its selective overexpression in tumor versus normal endothelial cells. The objective of this study was to detect the potential functions of TEM8 in osteosarcoma. Overall, TEM8 was mainly located in cytoplasm and was up-regulated in osteosarcoma compared to benign bone lesions and adjacent non tumor tissue (ANT). High TEM8 expression group had a significant lower overall survival rate than that in the low TEM8 expression group. TEM8 knock-down by siRNA or shRNA results in significant reduction of osteosarcoma cell growth and proliferation both in vitro and in vivo. Ablation of TEM8 led to increasing of p21 and p27 and suppression of cyclin D1 mediated by Erk1/2 activity. These findings suggest that down-regulation of TEM8 play an important role in the inhibition of tumorigenesis and development of osteosarcoma.
authors.affiliation: Department of Pathology, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Chuangjie
authors.initials: C
authors.lastname: Cao
authors.affiliation: Department of Pathology, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Pathology, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Leilei
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Respiratory, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Bai
authors.affiliation: Department of Pathology, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Yuefeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Pathology, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Department of Hepatology, Qilu Hospital of Shandong University, Jinan 250012, China.
authors.forename: Chengyun
authors.initials: C
authors.lastname: Dou
authors.affiliation: Department of Pathology, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Liantang
authors.initials: L
authors.lastname: Wang
mesh_terms: D001859:Bone Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D006801:Humans; D020935:MAP Kinase Signaling System; D008840:Microfilament Proteins; D009363:Neoplasm Proteins / Q000378:metabolism*; D012516:Osteosarcoma / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: C473287:ANTXR1 protein, human; D008840:Microfilament Proteins; D009363:Neoplasm Proteins; D011956:Receptors, Cell Surface