title: Hydrogen sulfide from a NaHS source attenuates dextran sulfate sodium (DSS)-induced inflammation via inhibiting nuclear factor-κB.
abstract: This study investigated the alleviating effects of hydrogen sulfide (H2S), derived from sodium hydrosulfide (NaHS), on inflammation induced by dextran sulfate sodium (DSS) in both in vivo and in vitro models. We found that NaHS injection markedly decreased rectal bleeding, diarrhea, and histological injury in DSS-challenged mice. NaHS (20 μmol/L) reversed DSS-induced inhibition in cell viability in Caco-2 cells and alleviated pro-inflammation cytokine expression in vivo and in vitro, indicating an anti-inflammatory function for H2S. It was also found that H2S may regulate cytokine expression by inhibiting the nuclear factor-κB (NF-κB) signaling pathway. In conclusion, our results demonstrated that H2S alleviated DSS-induced inflammation in vivo and in vitro and that the signal mechanism might be associated with the NF-κB signaling pathway.
authors.affiliation: Medical College, Qingdao University, Qingdao 266021, China.|Department of Gastroenterology, Yantai Municipal Laiyang Central Hospital, Yantai 265200, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Medical College, Qingdao University, Qingdao 266021, China.|Department of Gastroenterology, the Affiliated Hospital of Qingdao University, Qingdao 266071, China.
authors.forename: Xi-shuang
authors.initials: XS
authors.lastname: Liu
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D003092:Colitis / Q000139:chemically induced / Q000517:prevention & control*; D016207:Cytokines / Q000235:genetics; D016264:Dextran Sulfate / Q000633:toxicity*; D006801:Humans; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D007136:Immunoglobulins / Q000097:blood; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D015398:Signal Transduction / Q000187:drug effects; D013440:Sulfides / Q000494:pharmacology
chemical_list: D016207:Cytokines; D007136:Immunoglobulins; D016328:NF-kappa B; D013440:Sulfides; D016264:Dextran Sulfate; C025451:sodium bisulfide; D006862:Hydrogen Sulfide
keywords: Dextran sulfate sodium (DSS); Hydrogen sulfide; Inflammation; Nuclear factor-κB (NF-κB)