title: Microcin determinants are associated with B2 phylogroup of human fecal Escherichia coli isolates.
abstract: Escherichia coli strains are classified into four main phylogenetic groups (A, B1, B2, and D) and strains of these phylogroups differ in a number of characteristics. This study tested whether human fecal E. coli isolates belonging to different phylogroups differ in prevalence of bacteriocinogenic isolates and prevalence of individual bacteriocinogenic determinants. A set of 1283 fecal E. coli isolates from patients with different diseases was tested for the presence of DNA regions allowing classification into E. coli phylogroups and for the ability to produce bacteriocins (23 colicins and 7 microcins). Of the isolates tested, the most common was phylogroup B2 (38.3%) followed by phylogroups A (28.3%), D (26.3%) and B1 (7.2%). Altogether, 695 bacteriocin producers were identified representing 54.2% of all tested isolates. The highest prevalence of bacteriocin producers was found in group B2 (60.3%) and the lowest in group B1 (44.6%). Determinants encoding colicins E1, Ia, and microcin mV were most common in phylogroup A, determinants encoding microcins mM and mH47 were most common in phylogroup B2, and determinant encoding mB17 was most common in phylogroup D. The highest prevalence of bacteriocinogeny was found in phylogroup B2, suggesting that bacteriocinogeny and especially the synthesis of microcins was associated with virulent and resident E. coli strains.
authors.affiliation: Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Building A6, 625 00, Brno, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Micenková
authors.affiliation: Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Building A6, 625 00, Brno, Czech Republic.
authors.forename: Juraj
authors.initials: J
authors.lastname: Bosák
authors.affiliation: Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Building A6, 625 00, Brno, Czech Republic.
authors.forename: Barbora
authors.initials: B
authors.lastname: Štaudová
authors.affiliation: 2nd Department of Internal Medicine - Gastroenterology, Charles University in Prague, Faculty of Medicine at Hradec Králové, University Teaching Hospital, Sokolská 581, 500 05, Hradec Králové, Czech Republic.
authors.forename: Darina
authors.initials: D
authors.lastname: Kohoutová
authors.affiliation: Department of Immunology, Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic.
authors.forename: Darina
authors.initials: D
authors.lastname: Čejková
authors.affiliation: Department of Microbiology, Faculty of Medicine, Masaryk University and St. Anne's University Hospital, Pekarˇská 53, 656 91, Brno, Czech Republic.
authors.forename: Vladana
authors.initials: V
authors.lastname: Woznicová
authors.affiliation: Department of Clinical Microbiology, Faculty Hospital Brno, Jihlavská 20, 625 00, Brno, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Vrba
authors.affiliation: Department of Clinical Microbiology, Faculty Hospital Brno, Jihlavská 20, 625 00, Brno, Czech Republic.
authors.forename: Alena
authors.initials: A
authors.lastname: Ševčíková
authors.affiliation: 2nd Department of Internal Medicine - Gastroenterology, Charles University in Prague, Faculty of Medicine at Hradec Králové, University Teaching Hospital, Sokolská 581, 500 05, Hradec Králové, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Bureš
authors.affiliation: Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Building A6, 625 00, Brno, Czech Republic.
authors.forename: David
authors.initials: D
authors.lastname: Šmajs
mesh_terms: D001430:Bacteriocins / Q000378:metabolism*; D003087:Colicins / Q000378:metabolism*; D004926:Escherichia coli / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism* / Q000472:pathogenicity; D005243:Feces / Q000382:microbiology; D041981:Gastrointestinal Tract / Q000382:microbiology*; D006801:Humans; D010802:Phylogeny
chemical_list: D001430:Bacteriocins; D003087:Colicins; C020411:microcin
keywords: Bacteriocin; E. coli; colicin; microcin; phylogroup.