title: [Effects of high temperature on Bt protein content and nitrogen metabolic physiology in boll wall of Bt cotton].
abstract: Bt cotton cultivar Sikang 1 (a conventional cultivar) and Sikang 3 (a hybrid cultivar) from China, and 99B (a conventional cultivar) and Daiza 1 (a hybrid cultivar) from USA were selected as experimental materials, the ball wall Bt protein content and nitrogen metabolic physiology were investigated under different high temperature levels at peak boll stage. The results showed that the Bt protein content of boll wall decreased with the increasing temperature. Compared with the control (32 °C, the boll wall Bt protein content decreased significantly when the temperature was above 38 °C for the conventional cultivars and above 40 °C for the hybrid cultivars. The Bt protein contents of cultivar Sikang 1 and 99B decreased by 53.0% and 69.5% respectively with the temperature at 38 °C, and that of cultivar Sikang 3 and Daiza 1 decreased by 64.8% and 54.1% respectively with the temperature at 40 °C. Greater reductions in the boll wall soluble protein contents and GPT activities, larger increments for the boll wall free amino acid contents and proteinsase activities were also observed when the boll wall Bt protein content was significantly reduced. Therefore, high temperature resulted in the reduction of Bt protein synthesis and increase of the insecticidal protein degradation in the boll wall significantly, which caused the reductions in boll wall Bt protein content and insect resistance.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Eltayib H M A
authors.initials: EH
authors.lastname: Abidallah
authors.forename: Ming-ming
authors.initials: MM
authors.lastname: Hua
authors.forename: Li
authors.initials: L
authors.lastname: Heng
authors.forename: Chun-hua
authors.initials: CH
authors.lastname: Lyu
authors.forename: De-hua
authors.initials: DH
authors.lastname: Chen
mesh_terms: D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry*; D002681:China; D004731:Endotoxins / Q000737:chemistry*; D003368:Gossypium / Q000737:chemistry*; D006460:Hemolysin Proteins / Q000737:chemistry*; D006358:Hot Temperature; D007313:Insecta; D009584:Nitrogen / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000737:chemistry*
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D009584:Nitrogen