title: Potential Role of Placental Klotho in the Pathogenesis of Preeclampsia.
abstract: The aim of this study was to analyze the placental expression and allele status of promoter region of Klotho in association with preeclampsia, which represents the most common hypertensive disease of pregnancy. Klotho mRNA and protein levels were determined using real-time PCR and Western blot, respectively, in placental tissue samples obtained from 34 patients affected with preeclampsia and 34 controls. A PCR-based genotyping analysis was carried out in the promoter region of Klotho gene. Moreover, expression levels of pluripotency markers, Nanog and Oct4, and telomere length were assessed using real-time PCR. Klotho mRNA and protein levels were reduced in preeclamptic placentas compared with controls. -744delA single-nucleotide polymorphism was significantly associated with preeclampsia. In pathological placentas, there was a downregulation of pluripotency markers and a reduced telomere length. This study is the first to evaluate the placental expression level of Klotho in association with preeclampsia. Further analyses will clarify its role in the pathogenesis of this pregnancy hypertensive disorder.
authors.affiliation: Department of Clinical Sciences, Polytechnic University of Marche, Via Ranieri 65, 60131, Ancona, Italy.
authors.forename: Monia
authors.initials: M
authors.lastname: Cecati
authors.affiliation: Department of Clinical Sciences, Polytechnic University of Marche, Via Ranieri 65, 60131, Ancona, Italy.
authors.forename: Stefano Raffaele
authors.initials: SR
authors.lastname: Giannubilo
authors.affiliation: Department of Clinical Sciences, Polytechnic University of Marche, Via Ranieri 65, 60131, Ancona, Italy. f.saccucci@univpm.it.
authors.forename: Franca
authors.initials: F
authors.lastname: Saccucci
authors.affiliation: Department of Clinical Sciences, Polytechnic University of Marche, Via Ranieri 65, 60131, Ancona, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Sartini
authors.affiliation: Department of Clinical Sciences, Polytechnic University of Marche, Via Ranieri 65, 60131, Ancona, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Ciavattini
authors.affiliation: Department of Clinical Sciences, Polytechnic University of Marche, Via Ranieri 65, 60131, Ancona, Italy.|New York-Marche Structural Biology Center (NY-MaSBiC), Polytechnic University of Marche, Via Ranieri 65, 60131, Ancona, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Emanuelli
authors.affiliation: Department of Clinical Sciences, Polytechnic University of Marche, Via Ranieri 65, 60131, Ancona, Italy.
authors.forename: Andrea Luigi
authors.initials: AL
authors.lastname: Tranquilli
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D015536:Down-Regulation; D005260:Female; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D000090265:Klotho Proteins; D000071317:Nanog Homeobox Protein / Q000235:genetics / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D011225:Pre-Eclampsia / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D011247:Pregnancy; D059505:Telomere Homeostasis
chemical_list: D015415:Biomarkers; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D005966:Glucuronidase; D000090265:Klotho Proteins
keywords: Gene expression analysis; Hypertensive disease; Pregnancy; Single-nucleotide polymorphism; Telomere length