title: Association of interleukin 18-607A/C and -137C/G polymorphisms with oxidative stress in renal transplant recipients.
abstract: Objectives IL-18 mediates various inflammatory and oxidative responses including renal injury, fibrosis, and graft rejection. It has been reported that the promoter -607 and -137 polymorphisms of IL-18 influence the level of IL-18. This prospective observational study investigated the association between oxidative stress with IL-18-607 and -137 polymorphisms in renal transplant recipients. Patients and methods This study included 75 renal transplant recipients (28 female, 47 male) from living-related donors. Blood samples were collected immediately before and after transplantation at day 7 and month 1. Serum IL-18, creatinine, cystatin C, CRP, and oxidative stress markers (TOS, TAC) were measured. The Oxidative Stress Index (OSI) was calculated. Polymorphisms of the promoter region of the IL-18 gene, IL18-607A/C, and -137C/G were determined by analysis of a "real-time PCR/Melting curve". Results Serum creatinine, cystatin C, CRP, IL-18, TOS, and OSI levels significantly decreased after transplantation. Post-transplant levels of serum TAC and estimated GFR demonstrated consistent significant increases. Serum IL-18 levels were significantly higher in patients with IL-18-137 GG and IL-18-607 CC genotypes before transplantation. Conclusion Our results indicate that the IL-18-137 GG and -607 CC genotypes contribute to higher IL-18 levels; however, the influence of these polymorphisms on oxidative stress has not been observed.
authors.affiliation: a Department of Biochemistry, Faculty of Medicine , Akdeniz University , Antalya , Turkey ;
authors.forename: Fatih
authors.initials: F
authors.lastname: Davran
authors.affiliation: b Division of Nephrology, Department of Internal Medicine, Faculty of Medicine , Akdeniz University , Antalya , Turkey ;
authors.forename: Vural Taner
authors.initials: VT
authors.lastname: Yilmaz
authors.affiliation: a Department of Biochemistry, Faculty of Medicine , Akdeniz University , Antalya , Turkey ;
authors.forename: Bilge Karatoy
authors.initials: BK
authors.lastname: Erdem
authors.affiliation: c Department of Microbiology, Faculty of Medicine , Akdeniz University , Antalya , Turkey.
authors.forename: Meral
authors.initials: M
authors.lastname: Gultekin
authors.affiliation: b Division of Nephrology, Department of Internal Medicine, Faculty of Medicine , Akdeniz University , Antalya , Turkey ;
authors.forename: Gultekin
authors.initials: G
authors.lastname: Suleymanlar
authors.affiliation: a Department of Biochemistry, Faculty of Medicine , Akdeniz University , Antalya , Turkey ;
authors.forename: Halide
authors.initials: H
authors.lastname: Akbas
mesh_terms: D000328:Adult; D005260:Female; D006084:Graft Rejection / Q000175:diagnosis / Q000209:etiology / Q000235:genetics; D006801:Humans; D007249:Inflammation / Q000235:genetics; D020382:Interleukin-18 / Q000235:genetics*; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D007677:Kidney Function Tests / Q000379:methods; D016030:Kidney Transplantation / Q000009:adverse effects*; D019520:Living Donors; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000235:genetics; D019990:Perioperative Care / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013223:Statistics as Topic; D014421:Turkey
chemical_list: D020382:Interleukin-18
keywords: Interleukin-18 gene polymorphisms; oxidative stress; renal transplantation