title: Tetramethoxy-Aminorhodamine (TMARh): A Bichromophore, an Improved Fluorophore, and a pH Switch.
abstract: Rhodamine is one of the most widely used fluorescent dyes. Here, a new synthetic pathway to the popular dyes is reported and the effect of adding four methoxy groups to the molecular structure is investigated. Tetramethoxy-aminorhodamine (TMARh) is found to show superior pH switching compared to the rhodamine without the four methoxy groups, owing to changed properties of the dark "off" state and increased fluorescence intensity in the protonated "on" state.
authors.affiliation: Nano-Science Center & Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100, København Ø, Denmark.
authors.forename: Thomas Just
authors.identifier: 0000-0003-1491-5116
authors.initials: TJ
authors.lastname: Sørensen
authors.affiliation: Nano-Science Center & Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100, København Ø, Denmark.
authors.forename: Dong
authors.initials: D
authors.lastname: Shi
authors.affiliation: Nano-Science Center & Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100, København Ø, Denmark. bwl@nano.ku.dk.
authors.forename: Bo W
authors.identifier: 0000-0002-1120-3191
authors.initials: BW
authors.lastname: Laursen
mesh_terms: D000586:Amination; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D011522:Protons; D012235:Rhodamines / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D011522:Protons; D012235:Rhodamines
keywords: bichromophores; dyes/pigments; fluorescence; pH responsive dyes; photophysics; rhodamine