title: Histopathological, oxidative damage, biochemical, and genotoxicity alterations in hepatic rats exposed to deltamethrin: modulatory effects of garlic (Allium sativum).
abstract: Deltamethrin is a pesticide widely used as a synthetic pyrethroid. The aim of this study was undertaken to investigate the effects of deltamethrin to induce oxidative stress and changes in biochemical parameters, hepatotoxicity and genotoxicity in female rats following a short-term (30 days) oral exposure and attenuation of these effects by Allium sativum extract. Indeed, Allium sativum is known to be a good antioxidant food resource which helps destroy free radical particles. Our results showed that deltamethrin treatment caused an increase in liver enzyme activities of aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH); and hepatic lipid peroxidation (LPO) level. However, it induced a decrease in activities of hepatic catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) (p < 0.01). Allium sativum extract normalized significantly (p < 0.01) the mentioned parameters in deltamethrin-treated rats. For genotoxic evaluation, deltamethrin treatment showed a significant increase in frequencies of micronucleus in bone-marrow cells. Micronucleus formation is an indicator of chromosomal damage which has been increasingly used to detect the genotoxic potential of environmental pests. The present study showed that Allium sativum diminished the adverse effects induced by this synthetic pyrethroid insecticide.
authors.affiliation: a Animal Eco-Physiology Laboratory, Sciences Faculty of Sfax, Tunisia.
authors.forename: Marwa
authors.initials: M
authors.lastname: Ncir
authors.affiliation: b Laboratory of Human Molecular Genetics, Faculty of Medicine, Sfax, Tunisia.|c College of Pharmacy, Quassim University, Saudi Arabia.
authors.forename: Ghada
authors.initials: G
authors.lastname: Ben Salah
authors.affiliation: b Laboratory of Human Molecular Genetics, Faculty of Medicine, Sfax, Tunisia.
authors.forename: Hassen
authors.initials: H
authors.lastname: Kamoun
authors.affiliation: d Biochemistry Laboratory, CHU Habib Bourguiba of Sfax, Tunisia.
authors.forename: Fatma
authors.initials: F
authors.lastname: Makni Ayadi
authors.affiliation: e Histopathology Laboratory, CHU Habib Bourguiba of Sfax, Tunisia.
authors.forename: Abdelmajid
authors.initials: A
authors.lastname: Khabir
authors.affiliation: a Animal Eco-Physiology Laboratory, Sciences Faculty of Sfax, Tunisia.
authors.forename: Abdelfattah
authors.initials: A
authors.lastname: El Feki
authors.affiliation: a Animal Eco-Physiology Laboratory, Sciences Faculty of Sfax, Tunisia.
authors.forename: Mongi
authors.initials: M
authors.lastname: Saoudi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D004249:DNA Damage / Q000502:physiology*; D005260:Female; D005737:Garlic; D009570:Nitriles / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D011722:Pyrethrins / Q000633:toxicity*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D009570:Nitriles; D010936:Plant Extracts; D011722:Pyrethrins; C017180:decamethrin
keywords: Allium sativum; deltamethrin; deltaméthrine; genotoxicity; génotoxicité; oxidative stress; stress oxydant