title: Delimiting species in the Phacus longicauda complex (Euglenida) through morphological and molecular analyses.
abstract: Although Phacus longicauda is the type species of the genus Phacus and one of the most common species among autotrophic euglenids, its correct identification is nearly impossible. Over 30 morphologically similar taxa appear in the literature, but there are no good diagnostic features to distinguish them. Using environmental sampling and whole genome amplification, we delimited species within the Phacus longicauda complex. Morphological and molecular characters were analyzed for 36 strains isolated from environmental samples (mainly from Poland). DNA was obtained from a small number of cells (20-30) isolated with a micropipette from every sample (i.e., without setting up laboratory cultures), and phylogenetic analyses were based on variation in nSSU rDNA. Apart from Phacus longicauda, three other species (Phacus circumflexus, Phacus helikoides, and Phacus tortus) were distinguished. Phacus cordata comb. nov. Zakryś et M. Łukomska and Phacus rotunda comb. nov. Zakryś et M. Łukomska had their taxonomic ranks changed and two species new to science, Phacus cristatus sp. nov. Zakryś et M. Łukomska and Phacus crassus sp. nov. Zakryś et M. Łukomska, were described. For all verified species, diagnostic descriptions were amended and epitypes designated.
authors.affiliation: Department of Molecular Phylogenetics and Evolution, Faculty of Biology, University of Warsaw, Al. Ujazdowskie 4, Warszawa, PL-00-478, Poland.
authors.forename: Maja
authors.initials: M
authors.lastname: Łukomska-Kowalczyk
authors.affiliation: Department of Molecular Phylogenetics and Evolution, Faculty of Biology, University of Warsaw, Al. Ujazdowskie 4, Warszawa, PL-00-478, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Karnkowska
authors.affiliation: Department of Molecular Phylogenetics and Evolution, Faculty of Biology, University of Warsaw, Al. Ujazdowskie 4, Warszawa, PL-00-478, Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Milanowski
authors.affiliation: Department of Molecular Phylogenetics and Evolution, Faculty of Biology, University of Warsaw, Al. Ujazdowskie 4, Warszawa, PL-00-478, Poland.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Łach
authors.affiliation: Department of Molecular Phylogenetics and Evolution, Faculty of Biology, University of Warsaw, Al. Ujazdowskie 4, Warszawa, PL-00-478, Poland.
authors.forename: Bożena
authors.initials: B
authors.lastname: Zakryś
keywords: Euglenida; Phacus longicauda; environmental sampling; nSSU rDNA; new species; phylogeny; taxonomic revision