title: Therapeutic efficacy of amniotic membrane stem cells and adipose tissue stem cells in rats with chemically induced ovarian failure.
abstract: The present study was conducted to compare between the therapeutic efficacies of human amniotic membrane-derived stem cells (hAM-MSCs) vs. adipose tissue derived stem cells (AD-MSCs) in cyclophosphamide (CTX)-induced ovarian failure in rats. Forty-eight adult female rats were included in the study; 10 rats were used as control group. Thirty-eight rats were injected with CTX to induce ovarian failure and divided into four groups: ovarian failure (IOF) (IOF group), IOF + phosphate buffer saline (PBS group), IOF + hAM-MSCs group and IOF + AD-MSCs group. Serum levels of FSH and estradiol (E2) were assessed. Histopathological examination of the ovarian tissues was performed and quantitative gene expressions of Oct-4, Stra8 and integrin beta-1 genes were conducted by quantitative real time PCR. Results showed that IOF and IOF + PBS rat groups exhibited decreased ovarian follicles, increased interstitial fibrosis with significant decrease of serum E2, significant increase serum FSH level and significant down-regulation of Stra8 and integrin beta-1. In hAM-MSCs and AD-MSCs rat groups, there were increased follicles and corpora with evident the presence of oocytes, significant increase in serum E2, significant decrease in serum FSH levels (in hAM-MSCs treated group only) and significant up-regulation of the three studied genes with higher levels in hAM-MSCs treated rats group when compared to AD-MSCs treated rats group. In Conclusion, administration of either hAM-derived MSCs or AD-MSCs exerts a significant therapeutic efficacy in chemotherapy induced ovarian insult in rats. hAM-MSCs exert higher therapeutic efficacy as compared to AD-MSCs.
authors.affiliation: Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, P.O. Box 11562, Egypt.
authors.forename: Hanan
authors.initials: H
authors.lastname: Fouad
authors.affiliation: Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, P.O. Box 11562, Egypt.
authors.forename: Dina
authors.initials: D
authors.lastname: Sabry
authors.affiliation: Obstetrics and Gynecology Department, Faculty of Medicine, Cairo University, Egypt.
authors.forename: Khaled
authors.initials: K
authors.lastname: Elsetohy
authors.affiliation: Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, P.O. Box 11562, Egypt.
authors.forename: Naglaa
authors.initials: N
authors.lastname: Fathy
keywords: AD-MSCs; Chemotherapy; Ovarian failure; Ovarian insufficiency; hAM-MSCs