title: 2,3 Butanediol production in an obligate photoautotrophic cyanobacterium in dark conditions via diverse sugar consumption.
abstract: Cyanobacteria are under investigation as a means to utilize light energy to directly recycle CO2 into chemical compounds currently derived from petroleum. Any large-scale photosynthetic production scheme must rely on natural sunlight for energy, thereby limiting production time to only lighted hours during the day. Here, an obligate photoautotrophic cyanobacterium was engineered for enhanced production of 2,3-butanediol (23BD) in continuous light, 12h:12h light-dark diurnal, and continuous dark conditions via supplementation with glucose or xylose. This study achieved 23BD production under diurnal conditions comparable to production under continuous light conditions. The maximum 23BD titer was 3.0gL(-1) in 10d. Also achieving chemical production under dark conditions, this work enhances the feasibility of using cyanobacteria as industrial chemical-producing microbes.
authors.affiliation: Department of Chemistry, University of California, Davis, One Shields Ave, Davis, CA 95616, USA.
authors.forename: Jordan T
authors.initials: JT
authors.lastname: McEwen
authors.affiliation: Department of Chemistry, University of California, Davis, One Shields Ave, Davis, CA 95616, USA; Asahi Kasei Corporation, 2-1 Samejima, Fuji, Shizuoka 416-8501, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kanno
authors.affiliation: Department of Chemistry, University of California, Davis, One Shields Ave, Davis, CA 95616, USA. Electronic address: satsumi@ucdavis.edu.
authors.forename: Shota
authors.initials: S
authors.lastname: Atsumi
mesh_terms: D053898:Biosynthetic Pathways / Q000502:physiology; D002072:Butylene Glycols / Q000302:isolation & purification / Q000378:metabolism*; D000458:Cyanobacteria / Q000502:physiology* / Q000528:radiation effects; D003624:Darkness; D024861:Genetic Enhancement / Q000379:methods; D008027:Light; D060847:Metabolic Engineering / Q000379:methods*; D053858:Metabolic Networks and Pathways / Q000502:physiology*; D010788:Photosynthesis / Q000502:physiology* / Q000528:radiation effects; D000073893:Sugars / Q000378:metabolism*
chemical_list: D002072:Butylene Glycols; D000073893:Sugars; C026978:2,3-butylene glycol
keywords: 2,3 Butanediol; Cyanobacteria; Metabolic engineering; Obligate photoautotrophy