title: Genome sequence analysis of five Canadian isolates of strawberry mottle virus reveals extensive intra-species diversity and a longer RNA2 with increased coding capacity compared to a previously characterized European isolate.
abstract: In this study, we report the genome sequence of five isolates of strawberry mottle virus (family Secoviridae, order Picornavirales) from strawberry field samples with decline symptoms collected in Eastern Canada. The Canadian isolates differed from the previously characterized European isolate 1134 in that they had a longer RNA2, resulting in a 239-amino-acid extension of the C-terminal region of the polyprotein. Sequence analysis suggests that reassortment and recombination occurred among the isolates. Phylogenetic analysis revealed that the Canadian isolates are diverse, grouping in two separate branches along with isolates from Europe and the Americas.
authors.affiliation: Summerland Research and Development Centre, Agriculture and Agri-Food Canada, 4200 Highway 97, Summerland, BC, V0H 1Z0, Canada.
authors.forename: Basdeo
authors.initials: B
authors.lastname: Bhagwat
authors.affiliation: Fredericton Research and Development Centre, Agriculture and Agri-Food Canada, P.O. Box 20280, 850 Lincoln Road, Fredericton, NB, E3B 4Z7, Canada.
authors.forename: Virginia
authors.initials: V
authors.lastname: Dickison
authors.affiliation: Key Laboratory of Plant Virology of Fujian Province, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, People's Republic of China.|London Research and Development Centre, Agriculture and Agri-Food Canada, 1391 Sanford St., London, ON, N5V 4T3, Canada.
authors.forename: Xinlun
authors.initials: X
authors.lastname: Ding
authors.affiliation: Summerland Research and Development Centre, Agriculture and Agri-Food Canada, 4200 Highway 97, Summerland, BC, V0H 1Z0, Canada.
authors.forename: Melanie
authors.initials: M
authors.lastname: Walker
authors.affiliation: Summerland Research and Development Centre, Agriculture and Agri-Food Canada, 4200 Highway 97, Summerland, BC, V0H 1Z0, Canada.
authors.forename: Michael
authors.initials: M
authors.lastname: Bernardy
authors.affiliation: Summerland Research and Development Centre, Agriculture and Agri-Food Canada, 4200 Highway 97, Summerland, BC, V0H 1Z0, Canada.
authors.forename: Michel
authors.initials: M
authors.lastname: Bouthillier
authors.affiliation: Fredericton Research and Development Centre, Agriculture and Agri-Food Canada, P.O. Box 20280, 850 Lincoln Road, Fredericton, NB, E3B 4Z7, Canada.|Biology Program, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada.
authors.forename: Alexa
authors.initials: A
authors.lastname: Creelman
authors.affiliation: Summerland Research and Development Centre, Agriculture and Agri-Food Canada, 4200 Highway 97, Summerland, BC, V0H 1Z0, Canada.
authors.forename: Robyn
authors.initials: R
authors.lastname: DeYoung
authors.affiliation: London Research and Development Centre, Agriculture and Agri-Food Canada, 1391 Sanford St., London, ON, N5V 4T3, Canada.
authors.forename: Yinzi
authors.initials: Y
authors.lastname: Li
authors.affiliation: Fredericton Research and Development Centre, Agriculture and Agri-Food Canada, P.O. Box 20280, 850 Lincoln Road, Fredericton, NB, E3B 4Z7, Canada. Xianzhou.Nie@agr.gc.ca.
authors.forename: Xianzhou
authors.initials: X
authors.lastname: Nie
authors.affiliation: London Research and Development Centre, Agriculture and Agri-Food Canada, 1391 Sanford St., London, ON, N5V 4T3, Canada. Aiming.Wang@agr.gc.ca.
authors.forename: Aiming
authors.initials: A
authors.lastname: Wang
authors.affiliation: Summerland Research and Development Centre, Agriculture and Agri-Food Canada, 4200 Highway 97, Summerland, BC, V0H 1Z0, Canada. Yu.Xiang@agr.gc.ca.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiang
authors.affiliation: Summerland Research and Development Centre, Agriculture and Agri-Food Canada, 4200 Highway 97, Summerland, BC, V0H 1Z0, Canada. Helene.Sanfacon@agr.gc.ca.
authors.forename: Hélène
authors.identifier: http://orcid.org/0000-0003-3807-3590
authors.initials: H
authors.lastname: Sanfaçon
mesh_terms: D002170:Canada; D005060:Europe; D031985:Fragaria / Q000821:virology*; D014644:Genetic Variation; D016679:Genome, Viral; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012328:RNA Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics; D016865:Reassortant Viruses / Q000235:genetics / Q000302:isolation & purification; D011995:Recombination, Genetic
chemical_list: D012367:RNA, Viral