title: Degradation of oxytetracycline and its impacts on biogas-producing microbial community structure.
abstract: The effect of veterinary antibiotics in anaerobic digesters is a concern where methane production efficiency is highly dependent on microbial community structure. In this study, both anaerobic degradation of a common veterinary antibiotic, oxytetracycline (OTC), and its effects on an anaerobic digester microbial community were investigated. Qualitative and quantitative molecular tools were used to monitor changes in microbial community structure during a 60-day batch incubation period of cow manure with the addition of different concentrations of the antibiotic. Molecular data were interpreted by a further redundancy analysis as a multivariate statistics approach. At the end of the experiment, approximately 48, 33, and 17 % of the initially added 50, 100, and 200 mg l(-1) of OTC was still present in the serum bottles which reduced the biogas production via accumulation of some of the volatile fatty acids (VFAs). Biogas production was highly correlated with Methanobacteriales and Methanosarcinales gene copy numbers, and those parameters were negatively affected with oxytetracycline and VFA concentrations.
authors.affiliation: Institute of Environmental Sciences, Bogazici University, Bebek, 34342, Istanbul, Turkey. halil.coban@gmail.com.
authors.forename: Halil
authors.initials: H
authors.lastname: Coban
authors.affiliation: Institute of Environmental Sciences, Bogazici University, Bebek, 34342, Istanbul, Turkey.
authors.forename: Emine
authors.initials: E
authors.lastname: Ertekin
authors.affiliation: Department of Environmental Engineering, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey.
authors.forename: Orhan
authors.initials: O
authors.lastname: Ince
authors.affiliation: Institute of Environmental Sciences, Bogazici University, Bebek, 34342, Istanbul, Turkey.
authors.forename: Gokhan
authors.initials: G
authors.lastname: Turker
authors.affiliation: Institute of Environmental Sciences, Bogazici University, Bebek, 34342, Istanbul, Turkey.
authors.forename: Çağrı
authors.initials: Ç
authors.lastname: Akyol
authors.affiliation: Institute of Environmental Sciences, Bogazici University, Bebek, 34342, Istanbul, Turkey.
authors.forename: Bahar
authors.initials: B
authors.lastname: Ince
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D056804:Biofuels; D002417:Cattle; D008372:Manure / Q000382:microbiology; D010118:Oxytetracycline / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D056804:Biofuels; D008372:Manure; D012336:RNA, Ribosomal, 16S; D010118:Oxytetracycline
keywords: Biogas; Microbial community; Oxytetracycline; Real-time PCR