title: A game of tag: MAPS catches up on RNA interactomes.
abstract: In the last few decades, small regulatory RNA (sRNA) molecules emerged as key regulators in every kingdom of life. Resolving the full targetome of sRNAs has however remained a challenge. To address this, we used an in vivo tagging MS2-affinity purification protocol coupled with RNA sequencing technology, namely MAPS, to assemble full bacterial small RNAs targetomes. The impressive potential of MAPS has been supported by a number of reports. Here, we concisely overview RNA-tagging history that preceded the development of the MAPS assay and expose the range of possible uses of this technology.
authors.affiliation: a Department of Biochemistry , RNA Group, Université de Sherbrooke , Sherbrooke, Québec , Canada.
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Carrier
authors.affiliation: a Department of Biochemistry , RNA Group, Université de Sherbrooke , Sherbrooke, Québec , Canada.
authors.forename: David
authors.initials: D
authors.lastname: Lalaouna
authors.affiliation: a Department of Biochemistry , RNA Group, Université de Sherbrooke , Sherbrooke, Québec , Canada.
authors.forename: Eric
authors.initials: E
authors.lastname: Massé
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001419:Bacteria / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D007202:Indicators and Reagents / Q000737:chemistry; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism; D058727:RNA, Small Untranslated / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry; D013307:Streptomycin / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D001426:Bacterial Proteins; D007202:Indicators and Reagents; D012333:RNA, Messenger; D058727:RNA, Small Untranslated; D016601:RNA-Binding Proteins; D019809:Streptavidin; D013307:Streptomycin
keywords: Affinity purification; MS2; RNA purification; RNA sponge; RNA tag; RybB; RyhB; antisense; sRNA; tRNA