title: Hepatic and seric levels of purines in rats experimentally infected by Fasciola hepatica.
abstract: The aim of this study was to evaluate hepatic and seric levels of purines, as well as their breakdown products in rats infected by Fasciola hepatica on days 15 and 87 post-infection (PI). Rats were divided into two groups: uninfected (n = 10) and infected (n = 20). On day 15 (n = 5 for uninfected group and n = 10 for infected group) and 87 PI (n = 5 for uninfected group and n = 10 for infected group), animals were euthanized for sampling to evaluate levels of purines by high-performance liquid chromatography. In serum, ATP increased (P < 0.05) and ADP decreased (P < 0.05) on days 15 and 87 PI, while AMP increased (P < 0.05) only on day 15 PI. Hypoxanthine levels increased (P < 0.05) on days 15 and 87 PI, while adenosine and xanthine levels decreased and increased (P < 0.05), respectively, on day 87 PI. No difference was observed regarding seric inosine and uric acid (P > 0.05). Hepatic ATP, adenosine, and uric acid levels decreased (P < 0.05) on days 15 and 87 PI. AMP levels decreased (P < 0.05) on day 87 PI, while xanthine levels increased (P < 0.05) on day 15 PI in the liver. Also in the liver, hypoxanthine levels increased (P < 0.05) on day 15 PI and decreased (P < 0.05) on day 87 PI. On the other hand, there was no difference on hepatic ADP and inosine levels (P > 0.05). Therefore, it is possible to conclude that F. hepatica infection can change purine levels, which may be associated with an inflammatory process, and these alterations may influence fasciolosis pathogenesis.
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil.
authors.forename: Matheus D
authors.initials: MD
authors.lastname: Baldissera
authors.affiliation: Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil. ricardo.mendes@ifc-concordia.edu.br.
authors.forename: Ricardo E
authors.initials: RE
authors.lastname: Mendes
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil.
authors.forename: Pedro H
authors.initials: PH
authors.lastname: Doleski
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil.
authors.forename: Nathieli B
authors.initials: NB
authors.lastname: Bottari
authors.affiliation: Department of Morphological Science, Instituto de Ciências Básicas da Saúde (ICBA), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.|Department of Biochemistry, ICBS, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
authors.forename: Emerson A
authors.initials: EA
authors.lastname: Casali
authors.affiliation: Department of Morphological Science, Instituto de Ciências Básicas da Saúde (ICBA), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
authors.forename: Cesar Eduardo Jacintho
authors.initials: CE
authors.lastname: Moritz
authors.affiliation: Department of Morphological Science, Instituto de Ciências Básicas da Saúde (ICBA), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.|Department of Biochemistry, ICBS, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
authors.forename: Valesca V
authors.initials: VV
authors.lastname: Cardoso
authors.affiliation: Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil.
authors.forename: Luan C
authors.initials: LC
authors.lastname: Henker
authors.affiliation: Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Christ
authors.affiliation: Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil.
authors.forename: Fernanda A
authors.initials: FA
authors.lastname: Stedille
authors.affiliation: Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil.
authors.forename: Lenita M
authors.initials: LM
authors.lastname: Stefani
authors.affiliation: Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil. aleksandro.silva@udesc.br.
authors.forename: Aleksandro S
authors.initials: AS
authors.lastname: Da Silva
mesh_terms: D000227:Adenine Nucleotides / Q000097:blood / Q000378:metabolism; D000818:Animals; D005210:Fasciola hepatica / Q000502:physiology*; D005211:Fascioliasis / Q000469:parasitology*; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D011687:Purines / Q000097:blood / Q000378:metabolism; D051381:Rats
chemical_list: D000227:Adenine Nucleotides; D011687:Purines
keywords: Adenine nucleoside; Fasciolosis; Nucleotide adenine