title: Influence of 5% dextrose volume on amphotericin B deoxycholate preparation.
abstract: OBJECTIVE
  Preparation of amphotericin B deoxycholate (AmB-d) in different volumes of 5% dextrose (D5W) was studied to investigate a interesting phenomenon that AmB-d was easy to bring pipe blockage when diluted in 500 ml but not in 50 ml.
  
  
  METHODS
  AmB-d (25 mg/vial) in 50 ml, 250 ml or 500 ml D5W was prepared. Fluids were collected before and after infusion, then were assayed by validated high-performance liquid chromatography (HPLC) method. Light obscuration assay was used to detect the particles in transfusions.
  
  
  RESULTS
  pH values of different volumes of D5W were all about 3.7, which was lower than the requirement of AmB-d package insert (pH > 4.2). The number of insoluble particles >10 μm/25 μm in 25 mg/500 ml infusions exceeded China Pharmacopoeia limit. Filters in 25 mg/500 ml infusion set were full of AmB-d after dripping slowly for 6 h, and 331.3 ml solution was left in the bottles and only 11.3% of AmB-d could flow out. Whereas the AmB-d infusion consists of 25 mg/50 ml, 25 mg/250 ml and 50 mg/500 ml could meet with China Pharmacopoeia standards, and they flowed out easily and completely.
  
  
  CONCLUSIONS
  In practice, 25 mg/250 ml and 50 mg/500 ml would be more suitable for clinical use, rather than 25 mg/500 ml. We provided a convenient method for AmB-d preparation.
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors.forename: Pingping
authors.initials: P
authors.lastname: Sun
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors.forename: Zhihao
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Chen
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors.forename: Shuxia
authors.initials: S
authors.lastname: Li
mesh_terms: D000666:Amphotericin B / Q000008:administration & dosage / Q000737:chemistry* / Q000592:standards; D000935:Antifungal Agents / Q000008:administration & dosage / Q000737:chemistry* / Q000592:standards; D002851:Chromatography, High Pressure Liquid; D003840:Deoxycholic Acid / Q000008:administration & dosage / Q000737:chemistry* / Q000592:standards; D004338:Drug Combinations; D004339:Drug Compounding; D005947:Glucose / Q000737:chemistry* / Q000592:standards; D006863:Hydrogen-Ion Concentration; D007263:Infusions, Parenteral; D010316:Particle Size; D019999:Pharmaceutical Solutions; D012995:Solubility
chemical_list: D000935:Antifungal Agents; D004338:Drug Combinations; D019999:Pharmaceutical Solutions; D003840:Deoxycholic Acid; D000666:Amphotericin B; C059765:amphotericin B, deoxycholate drug combination; D005947:Glucose
keywords: 5% dextrose; amphotericin B deoxycholate; high-performance liquid chromatography; preparation; solubility