title: Identifying inhibitory effects of lignocellulosic by-products on growth of lactic acid producing micro-organisms using a rapid small-scale screening method.
abstract: Sugars obtained from pretreated lignocellulose are interesting as substrate for the production of lactic acid in fermentation processes. However, by-products formed during pretreatment of lignocellulose can inhibit microbial growth. In this study, a small-scale rapid screening method was used to identify inhibitory effects of single and combined by-products on growth of lactic acid producing micro-organisms. The small-scale screening was performed in 48-well plates using 5 bacterial species and 12 by-products. Large differences were observed in inhibitory effects of by-products between different species. Predictions can be made for growth behaviour of different micro-organisms on acid pretreated or alkaline pretreated bagasse substrates using data from the small-scale screening. Both individual and combined inhibition effects were shown to be important parameters to predict growth. Synergy between coumaric acid, formic acid and acetic acid is a key inhibitory parameter in alkaline pretreated lignocellulose, while furfural is a key inhibitor in acid pretreated lignocellulose.
authors.affiliation: Food and Biobased Research, Wageningen University and Research Centre, PO Box 17, 6700 AA Wageningen, Netherlands; Bioprocess Engineering, Wageningen University and Research Centre, PO Box 16, 6700 AA Wageningen, Netherlands. Electronic address: edwin.vanderpol@wur.nl.
authors.forename: Edwin C
authors.initials: EC
authors.lastname: van der Pol
authors.affiliation: Bioprocess Engineering, Wageningen University and Research Centre, PO Box 16, 6700 AA Wageningen, Netherlands.
authors.forename: Evelien
authors.initials: E
authors.lastname: Vaessen
authors.affiliation: Bioprocess Engineering, Wageningen University and Research Centre, PO Box 16, 6700 AA Wageningen, Netherlands.
authors.forename: Ruud A
authors.initials: RA
authors.lastname: Weusthuis
authors.affiliation: Food and Biobased Research, Wageningen University and Research Centre, PO Box 17, 6700 AA Wageningen, Netherlands; Bioprocess Engineering, Wageningen University and Research Centre, PO Box 16, 6700 AA Wageningen, Netherlands.
authors.forename: Gerrit
authors.initials: G
authors.lastname: Eggink
mesh_terms: D000143:Acids / Q000494:pharmacology; D000468:Alkalies / Q000494:pharmacology; D000693:Anaerobiosis / Q000187:drug effects; D001709:Biotechnology / Q000379:methods*; D005285:Fermentation / Q000187:drug effects; D019344:Lactic Acid / Q000096:biosynthesis*; D007778:Lactobacillus / Q000187:drug effects / Q000254:growth & development*; D008031:Lignin / Q000378:metabolism*
chemical_list: D000143:Acids; D000468:Alkalies; C036909:lignocellulose; D019344:Lactic Acid; D008031:Lignin
keywords: Bacteria; Inhibition; Lactic acid; Lignocellulosic by-products; Screening