title: Concomitant use of Congo red staining and confocal laser scanning microscopy to detect amyloidosis in oral biopsy: A clinicopathological study of 16 patients.
abstract: Twenty oral biopsies from 16 patients were analyzed both by traditional microscopy and by confocal laser scanning microscopy. Using conventional histopathological techniques, the diagnosis of amyloidosis was confirmed only in 15 biopsies. Using confocal laser scanning microscopy, amyloid deposits were detected in all of the samples. The current study shows that confocal laser scanning analysis helps to identify minimal amyloid deposits that could be overlooked using traditional microscopy, thus raising the sensitivity of oral biopsy up to 100%.
authors.affiliation: a Interdisciplinary Department of Medicine , Complex Operating Unit of Odontostomatology, "Aldo Moro" University of Bari , Bari , Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Scivetti
authors.affiliation: a Interdisciplinary Department of Medicine , Complex Operating Unit of Odontostomatology, "Aldo Moro" University of Bari , Bari , Italy.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Favia
authors.affiliation: a Interdisciplinary Department of Medicine , Complex Operating Unit of Odontostomatology, "Aldo Moro" University of Bari , Bari , Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Fatone
authors.affiliation: b Department of Emergency and Organ Transplantation , Operating Unit of Pathological Anatomy, "Aldo Moro" University of Bari , Bari , Italy.
authors.forename: Eugenio
authors.initials: E
authors.lastname: Maiorano
authors.affiliation: a Interdisciplinary Department of Medicine , Complex Operating Unit of Odontostomatology, "Aldo Moro" University of Bari , Bari , Italy.
authors.forename: Vito
authors.initials: V
authors.lastname: Crincoli
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000682:Amyloid / Q000032:analysis*; D000686:Amyloidosis / Q000378:metabolism* / Q000473:pathology; D015415:Biomarkers / Q000032:analysis; D001706:Biopsy; D004396:Coloring Agents; D003224:Congo Red; D005260:Female; D006801:Humans; D008297:Male; D018613:Microscopy, Confocal; D008875:Middle Aged; D009055:Mouth / Q000737:chemistry* / Q000473:pathology; D058225:Plaque, Amyloid; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D013194:Staining and Labeling / Q000379:methods*; D055815:Young Adult
chemical_list: D000682:Amyloid; D015415:Biomarkers; D004396:Coloring Agents; D003224:Congo Red
keywords: Amyloidosis; confocal laser scanning microscopy; oral biopsy