title: Proteomic analysis and identification of cell surface-associated proteins of Clostridium chauvoei.
abstract: Blackleg is a highly fatal disease of cattle and sheep, caused by Clostridium chauvoei, a Gram positive, anaerobic, spore forming bacteria. Cell surface-associated proteins play a major role in inducing the protective immunity. However, the identity of a majority of cell surface-associated proteins of C. chauvoei is not known. In the present investigation, we have used SDS-PAGE, 2D-gel electrophoresis and Western blotting followed by mass spectrometry to identify cell surface-associated proteins of C. chauvoei. Among the identified proteins, which have shown to offer protective antigencity in other bacteria, Enolase, Chaperonin, Ribosomal protein L10, Glycosyl Hydrolase and Flavoprotein were characterized by sequencing and their overexpression in Escherichia coli. In conclusion, cell surface-associated proteins were identified using proteomic approach and the genes for the immunoreactive proteins were expressed, which may prove to be potential diagnostic or vaccine candidates.
authors.affiliation: Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India.
authors.forename: Usharani
authors.initials: U
authors.lastname: Jayaramaiah
authors.affiliation: Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India.
authors.forename: Neetu
authors.initials: N
authors.lastname: Singh
authors.affiliation: Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India.
authors.forename: Sabarinath
authors.initials: S
authors.lastname: Thankappan
authors.affiliation: Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal 132001, India.
authors.forename: Ashok Kumar
authors.initials: AK
authors.lastname: Mohanty
authors.affiliation: Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India.
authors.forename: Pallab
authors.initials: P
authors.lastname: Chaudhuri
authors.affiliation: Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India.
authors.forename: Vijendra Pal
authors.initials: VP
authors.lastname: Singh
authors.affiliation: Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India. Electronic address: vkn111@gmail.com.
authors.forename: Viswas Konasagara
authors.initials: VK
authors.lastname: Nagaleekar
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D015153:Blotting, Western; D018833:Chaperonins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D003001:Cloning, Molecular; D046975:Clostridium chauvoei / Q000235:genetics* / Q000276:immunology; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005420:Flavoproteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D015870:Gene Expression; D007106:Immune Sera / Q000737:chemistry / Q000302:isolation & purification; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D010751:Phosphopyruvate Hydratase / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D040901:Proteomics; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D000080882:Ribosomal Protein L10; D012269:Ribosomal Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D017422:Sequence Analysis, DNA
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D005420:Flavoproteins; D007106:Immune Sera; D008565:Membrane Proteins; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; D018833:Chaperonins; D010751:Phosphopyruvate Hydratase
keywords: Cell surface-associated proteins; Clostridium chauvoei; Proteomics