title: Integration-Free Reprogramming of Lamina Propria Progenitor Cells.
abstract: Producing induced pluripotent stem cells (iPSCs) from human tissue for use in personalized medicine strategies or therapeutic testing is at the forefront of medicine. Therefore, identifying a source of cells to reprogram that is easily accessible via a simple noninvasive procedure is of great clinical importance. Reprogramming these cells to iPSCs through nonintegrating methods for genetic manipulation is paramount for regenerative purposes. Here, we demonstrate reprogramming of oral mucosal lamina propria progenitor cells from patients undergoing routine dental treatment. Reprogramming was performed utilizing nonintegrating plasmids containing all 6 pluripotency genes (OCT4, SOX2, KLF4, NANOG, LIN28, and cMYC). Resulting iPSCs lacked genetic integration of the vector genes and had the ability to differentiate down mesoderm, ectoderm, and endoderm lineages, demonstrating pluripotency. In conclusion, oral mucosal lamina propria progenitor cells represent a source of cells that can be obtained with minimal invasion, as they can be taken concurrently with routine treatments. The resulting integration-free iPSCs therefore have great potential for use in personalized medicine strategies.
authors.affiliation: Wound Biology Group, Cardiff Institute of Tissue Engineering and Repair, Oral and Biomedical Sciences, School of Dentistry, Cardiff University, Cardiff, Wales, UK howardjonesRA1@cf.ac.uk.
authors.forename: R A
authors.initials: RA
authors.lastname: Howard-Jones
authors.affiliation: Wound Biology Group, Cardiff Institute of Tissue Engineering and Repair, Oral and Biomedical Sciences, School of Dentistry, Cardiff University, Cardiff, Wales, UK.
authors.forename: O K Y
authors.initials: OK
authors.lastname: Cheung
authors.affiliation: Wound Biology Group, Cardiff Institute of Tissue Engineering and Repair, Oral and Biomedical Sciences, School of Dentistry, Cardiff University, Cardiff, Wales, UK.
authors.forename: A
authors.initials: A
authors.lastname: Glen
authors.affiliation: School of Biosciences, College of Biomedical and Life Sciences, Cardiff University, Cardiff, Wales, UK.
authors.forename: N D
authors.initials: ND
authors.lastname: Allen
authors.affiliation: Wound Biology Group, Cardiff Institute of Tissue Engineering and Repair, Oral and Biomedical Sciences, School of Dentistry, Cardiff University, Cardiff, Wales, UK.
authors.forename: P
authors.initials: P
authors.lastname: Stephens
mesh_terms: D002478:Cells, Cultured; D065150:Cellular Reprogramming / Q000502:physiology*; D006801:Humans; D000090062:Kruppel-Like Factor 4; D009061:Mouth Mucosa / Q000166:cytology* / Q000502:physiology; D010957:Plasmids / Q000235:genetics; D039904:Pluripotent Stem Cells / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000502:physiology*
keywords: IPS cells; hiPSCs; human induced pluripotent stem cells; oral mucosa; regenerative medicine; stem cells