title: Facile analysis of contents and compositions of the chondroitin sulfate/dermatan sulfate hybrid chain in shark and ray tissues.
abstract: The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain was extracted from specific tissues of several kinds of sharks and rays. The contents and sulfation patterns of the CS/DS hybrid chain were precisely analyzed by digestion with chondroitinases ABC and AC. All samples predominantly contained the A- and C-units. Furthermore, all samples characteristically contained the D-unit. Species-specific differences were observed in the contents of the CS/DS hybrid chain, which were the highest in Mako and Blue sharks and Sharpspine skates, but were lower in Hammerhead sharks. Marked differences were observed in the ratio of the C-unit/A-unit between sharks and rays. The contents of the CS/DS hybrid chain and the ratio of the C-unit/A-unit may be related to an oxidative stress-decreasing ability.
authors.affiliation: Organization for Regional Industrial Academic Cooperation, Tottori University, 4-101 Koyamacho-Minami, Tottori 680-8550, Japan. Electronic address: ntakeda@rs.tottori-u.ac.jp.
authors.forename: Naoko
authors.initials: N
authors.lastname: Takeda
authors.affiliation: Department of Regional Environment, Faculty of Regional Sciences, Tottori University, 4-101 Koyamacho-Minami, Tottori 680-8551, Japan. Electronic address: horais@rs.tottori-u.ac.jp.
authors.forename: Sawako
authors.initials: S
authors.lastname: Horai
authors.affiliation: Department of Regional Environment, Faculty of Regional Sciences, Tottori University, 4-101 Koyamacho-Minami, Tottori 680-8551, Japan. Electronic address: jtamura@rs.tottori-u.ac.jp.
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Tamura
mesh_terms: D000818:Animals; D019765:Chondroitin ABC Lyase / Q000737:chemistry; D002808:Chondroitin Lyases / Q000737:chemistry; D002809:Chondroitin Sulfates / Q000737:chemistry*; D003871:Dermatan Sulfate / Q000737:chemistry*; D018384:Oxidative Stress; D012754:Sharks; D012860:Skates, Fish
chemical_list: D003871:Dermatan Sulfate; D002809:Chondroitin Sulfates; D002808:Chondroitin Lyases; D019765:Chondroitin ABC Lyase
keywords: Chondroitin sulfate; Dermatan sulfate; Glycosaminoglycan; Ray; Shark