title: Composition and biological activities of the essential oil of Piper corcovadensis (Miq.) C. DC (Piperaceae).
abstract: Essential oil from fresh leaves of the shrub Piper corcovadensis (Miq.) C. DC was obtained in 0.21% (w/w) yield by hydrodistillation in a Clevenger type apparatus. Thirty-one components, accounting for 96.61% of the leaf oil, were identified by gas chromatography-mass spectrometry. The major constituents of the oil were 1-butyl-3,4-methylenedioxybenzene (30.62%), terpinolene (17.44%), trans -caryophyllene (6.27%), α-pinene (5.92%), δ-cadinene (4.92%), and Limonene (4.46%). Bioassays against larvae of the Dengue mosquito (Aedes aegypti) revealed that leaf oil (LC50 = 30.52 ppm), terpinolene (LC50 = 31.16 ppm), and pure 1-butyl-3,4-methylenedioxybenzene (LC50 = 22.1 ppm) possessed larvicidal activities and are able to interfere with the activity of proteases from L4 gut enzymes. Additionally, the essential oil exhibited a strong oviposition deterrent activity at 50 and 5 ppm. This paper constitutes the first report of biological activities associated with the essential oil of leaves of P. corcovadensis.
authors.affiliation: Departamento de Química Fundamental, Universidade Federal de Pernambuco, 50740-560, Recife, PE, Brazil.
authors.forename: Marcelo Felipe Rodrigues
authors.initials: MF
authors.lastname: da Silva
authors.affiliation: Departamento de Química Fundamental, Universidade Federal de Pernambuco, 50740-560, Recife, PE, Brazil.
authors.forename: Patrícia Cristina
authors.initials: PC
authors.lastname: Bezerra-Silva
authors.affiliation: Departamento de Química Fundamental, Universidade Federal de Pernambuco, 50740-560, Recife, PE, Brazil.
authors.forename: Camila Soledade
authors.initials: CS
authors.lastname: de Lira
authors.affiliation: Departamento de Química Fundamental, Universidade Federal de Pernambuco, 50740-560, Recife, PE, Brazil.
authors.forename: Bheatriz Nunes
authors.initials: BN
authors.lastname: de Lima Albuquerque
authors.affiliation: Departamento de Bioquímica, Universidade Federal de Pernambuco, 50670-420, Recife, PE, Brazil; Jardim Botânico de Recife, Pernambuco, 54240-450, Recife, PE, Brazil.
authors.forename: Afonso Cordeiro
authors.initials: AC
authors.lastname: Agra Neto
authors.affiliation: Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, 52171-900, Recife, PE, Brazil.
authors.forename: Emmanuel Viana
authors.initials: EV
authors.lastname: Pontual
authors.affiliation: Jardim Botânico de Recife, Pernambuco, 54240-450, Recife, PE, Brazil.
authors.forename: Jefferson Rodrigues
authors.initials: JR
authors.lastname: Maciel
authors.affiliation: Departamento de Bioquímica, Universidade Federal de Pernambuco, 50670-420, Recife, PE, Brazil.
authors.forename: Patrícia Maria Guedes
authors.initials: PM
authors.lastname: Paiva
authors.affiliation: Departamento de Química Fundamental, Universidade Federal de Pernambuco, 50740-560, Recife, PE, Brazil. Electronic address: dmafn@ufpe.br.
authors.forename: Daniela Maria do Amaral Ferraz
authors.initials: DM
authors.lastname: Navarro
mesh_terms: D000330:Aedes / Q000187:drug effects / Q000502:physiology; D000818:Animals; D000081008:Bicyclic Monoterpenes; D001681:Biological Assay; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes / Q000032:analysis; D003715:Dengue / Q000635:transmission; D004591:Electrophoresis, Polyacrylamide Gel; D008401:Gas Chromatography-Mass Spectrometry; D007814:Larva / Q000201:enzymology; D000077222:Limonene; D039821:Monoterpenes / Q000032:analysis; D000072138:Mosquito Vectors / Q000187:drug effects / Q000502:physiology; D009822:Oils, Volatile / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D010058:Oviposition / Q000187:drug effects; D010447:Peptide Hydrolases / Q000032:analysis; D031701:Piper / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D000081223:Polycyclic Sesquiterpenes; D011480:Protease Inhibitors / Q000494:pharmacology; D012717:Sesquiterpenes / Q000032:analysis; D013729:Terpenes / Q000032:analysis; D014357:Trypsin / Q000187:drug effects / Q000378:metabolism
chemical_list: D000081008:Bicyclic Monoterpenes; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D039821:Monoterpenes; D009822:Oils, Volatile; D000081223:Polycyclic Sesquiterpenes; D011480:Protease Inhibitors; D012717:Sesquiterpenes; D013729:Terpenes; C000607799:delta-cadinene; D000077222:Limonene; C024714:caryophyllene; D010447:Peptide Hydrolases; D014357:Trypsin; C005451:alpha-pinene; C027009:terpinolene
keywords: 1-Butyl-3,4-methylenedioxybenzene; Essential oil; Larvicidal activity; Oviposition activity; Piper corcovadensis; Piperaceae; Proteases enzymes