title: Surface Analysis of Gold Nanoparticles Functionalized with Thiol-Modified Glucose SAMs for Biosensor Applications.
abstract: In this work, Time of Flight Secondary Ion Mass Spectrometry (ToF-SIMS), Principal Component Analysis (PCA) and X-ray Photoelectron Spectroscopy (XPS) have been used to characterize the surface chemistry of gold substrates before and after functionalization with thiol-modified glucose self-assembled monolayers and subsequent biochemical specific recognition of maltose binding protein (MBP). The results indicate that the surface functionalization is achieved both on flat and nanoparticles gold substrates thus showing the potential of the developed system as biodetection platform. Moreover, the method presented here has been found to be a sound and valid approach to characterize the surface chemistry of nanoparticles functionalized with large molecules. Both techniques were proved to be very useful tools for monitoring all the functionalization steps, including the investigation of the biological behavior of the glucose-modified particles in the presence of the maltose binding protein.
authors.affiliation: European Commission, Joint Research Centre, Institute for Health and Consumer ProtectionIspra, Italy; Istituto di Fisica dei Plasmi, Consiglio Nazionale delle RicercheMilano, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Spampinato
authors.affiliation: European Commission, Joint Research Centre, Institute for Health and Consumer ProtectionIspra, Italy; Technical Department, Nanoimmunotech S.LZaragoza, Spain.
authors.forename: Maria Antonietta
authors.initials: MA
authors.lastname: Parracino
authors.affiliation: European Commission, Joint Research Centre, Institute for Health and Consumer Protection Ispra, Italy.
authors.forename: Rita
authors.initials: R
authors.lastname: La Spina
authors.affiliation: European Commission, Joint Research Centre, Institute for Health and Consumer Protection Ispra, Italy.
authors.forename: Francois
authors.initials: F
authors.lastname: Rossi
authors.affiliation: European Commission, Joint Research Centre, Institute for Health and Consumer Protection Ispra, Italy.
authors.forename: Giacomo
authors.initials: G
authors.lastname: Ceccone
keywords: SAMs; ToF-SIMS; XPS; biodetection; gold nanoparticles; surface functionalization