title: Neuronal pannexin-1 channels are not molecular routes of water influx during spreading depolarization-induced dendritic beading.
abstract: Spreading depolarization-induced focal dendritic swelling (beading) is an early hallmark of neuronal cytotoxic edema. Pyramidal neurons lack membrane-bound aquaporins posing a question of how water enters neurons during spreading depolarization. Recently, we have identified chloride-coupled transport mechanisms that can, at least in part, participate in dendritic beading. Yet transporter-mediated ion and water fluxes could be paralleled by water entry through additional pathways such as large-pore pannexin-1 channels opened by spreading depolarization. Using real-time in vivo two-photon imaging in mice with pharmacological inhibition or conditional genetic deletion of pannexin-1, we showed that pannexin-1 channels are not required for spreading depolarization-induced focal dendritic swelling.
authors.affiliation: 1 Brain and Behavior Discovery Institute, Medical College of Georgia, Augusta, GA, USA.
authors.forename: Jeremy
authors.initials: J
authors.lastname: Sword
authors.affiliation: 1 Brain and Behavior Discovery Institute, Medical College of Georgia, Augusta, GA, USA.
authors.forename: Deborah
authors.initials: D
authors.lastname: Croom
authors.affiliation: 1 Brain and Behavior Discovery Institute, Medical College of Georgia, Augusta, GA, USA.
authors.forename: Phil L
authors.initials: PL
authors.lastname: Wang
authors.affiliation: 2 Hotchkiss Brain Institute, Department of Cell Biology and Anatomy, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Roger J
authors.initials: RJ
authors.lastname: Thompson
authors.affiliation: 1 Brain and Behavior Discovery Institute, Medical College of Georgia, Augusta, GA, USA.|3 Department of Neurosurgery, Medical College of Georgia, Augusta, GA, USA.
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Kirov
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism / Q000473:pathology; D001692:Biological Transport; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D017630:Connexins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D013181:Cortical Spreading Depression / Q000187:drug effects / Q000502:physiology*; D003712:Dendrites / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D015767:Mefloquine / Q000494:pharmacology; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D036641:Microscopy, Fluorescence, Multiphoton; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D014867:Water / Q000378:metabolism*
chemical_list: D017630:Connexins; D009419:Nerve Tissue Proteins; C503631:Panx1 protein, mouse; D014867:Water; D015767:Mefloquine
keywords: Conditional pannexin-1 knock-out; mefloquine; neuronal swelling; two-photon imaging; viral vectors