title: Molecular mechanism of brain impairment caused by drinking-acquired fluorosis and selenium intervention.
abstract: This study investigated the molecular mechanism of brain impairment induced by drinking fluoridated water and selenium intervention. Results showed that the learning and memory of rats in NaF group significantly decreased. Moreover, the number of apoptotic cells, the expression levels of Cytc mRNA and protein, and the expression levels of Caspase-9 and Caspase-3 mRNA significantly increased; by contrast, Caspase-9 and Caspase-3 protein levels significantly decreased. Compared with the NaF group, the mRNA levels of Cytc and Caspase-9, as well as the protein levels of Cytc in NaF+Se group, significantly decreased. Conversely, the protein levels of Caspase-3 and Caspase-9, as well as the mRNA levels of Caspase-3, significantly increased. Thus, the mitochondrial CytC-Caspase-9-Caspase-3 apoptosis pathway in the hippocampus was one of the mechanisms leading to fluorosis-induced brain damage. Furthermore, the Cytc signaling molecules were possibly the key target molecules in fluorosis-induced apoptosis, and selenium could alleviate fluorosis-induced brain injury.
authors.affiliation: College of Chemistry and Life Science at Zhejiang Normal University, Jinhua 321004, PR China.
authors.forename: Xiangren
authors.initials: X
authors.lastname: Zheng
authors.affiliation: College of Chemistry and Life Science at Zhejiang Normal University, Jinhua 321004, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: College of Chemistry and Life Science at Zhejiang Normal University, Jinhua 321004, PR China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Ke
authors.affiliation: College of Sports and Health Science, Zhejiang Normal University, Jinhua 321004, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ouyang
authors.affiliation: College of Chemistry and Life Science at Zhejiang Normal University, Jinhua 321004, PR China; College of Xing Zhi, Zhejiang Normal University, Jinhua 321004, PR China. Electronic address: zzg@zjnu.cn.
authors.forename: Zigui
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis; D001921:Brain / Q000187:drug effects / Q000502:physiology*; D053148:Caspase 3 / Q000235:genetics; D053453:Caspase 9 / Q000235:genetics; D060766:Drinking Water; D005459:Fluorides / Q000633:toxicity*; D009050:Fluorosis, Dental / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012643:Selenium / Q000494:pharmacology*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D060766:Drinking Water; D018696:Neuroprotective Agents; D020011:Protective Agents; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D053148:Caspase 3; D053453:Caspase 9; D012643:Selenium; D005459:Fluorides
keywords: Apoptosis; Brain damage; Fluorosis; Hippocampus; Selenium