title: Fully distributed absolute blood flow velocity measurement for middle cerebral arteries using Doppler optical coherence tomography.
abstract: Doppler optical coherence tomography (DOCT) is considered one of the most promising functional imaging modalities for neuro biology research and has demonstrated the ability to quantify cerebral blood flow velocity at a high accuracy. However, the measurement of total absolute blood flow velocity (BFV) of major cerebral arteries is still a difficult problem since it is related to vessel geometry. In this paper, we present a volumetric vessel reconstruction approach that is capable of measuring the absolute BFV distributed along the entire middle cerebral artery (MCA) within a large field-of-view. The Doppler angle at each point of the MCA, representing the vessel geometry, is derived analytically by localizing the artery from pure DOCT images through vessel segmentation and skeletonization. Our approach could achieve automatic quantification of the fully distributed absolute BFV across different vessel branches. Experiments on rodents using swept-source optical coherence tomography showed that our approach was able to reveal the consequences of permanent MCA occlusion with absolute BFV measurement.
authors.affiliation: Institute of Optical Communication Engineering and College of Engineering and Applied Sciences, Nanjing University, Nanjing, Jiangsu, 210093, China; Beckman Laser Institute, University of California, Irvine, Irvine, California 92612, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Qi
authors.affiliation: Beckman Laser Institute, University of California, Irvine, Irvine, California 92612, USA; Department of Biomedical Engineering, University of California, Irvine, Irvine, California 92697, USA.
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Neurobiology and Behavior, University of California, Irvine, Irvine, California, USA; The Center for the Neurobiology of Learning and Memory, University of California, Irvine, Irvine, California, USA.
authors.forename: Aneeka M
authors.initials: AM
authors.lastname: Hancock
authors.affiliation: Beckman Laser Institute, University of California, Irvine, Irvine, California 92612, USA.
authors.forename: Cuixia
authors.initials: C
authors.lastname: Dai
authors.affiliation: Institute of Optical Communication Engineering and College of Engineering and Applied Sciences, Nanjing University, Nanjing, Jiangsu, 210093, China.
authors.forename: Xuping
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Biomedical Engineering, University of California, Irvine, Irvine, California 92697, USA; Department of Neurobiology and Behavior, University of California, Irvine, Irvine, California, USA; The Center for the Neurobiology of Learning and Memory, University of California, Irvine, Irvine, California, USA.
authors.forename: Ron D
authors.initials: RD
authors.lastname: Frostig
authors.affiliation: Beckman Laser Institute, University of California, Irvine, Irvine, California 92612, USA; Department of Biomedical Engineering, University of California, Irvine, Irvine, California 92697, USA.
authors.forename: Zhongping
authors.initials: Z
authors.lastname: Chen
keywords: (100.2960) Image analysis; (110.4500) Optical coherence tomography; (170.2655) Functional monitoring and imaging