title: Isolation of a non-fermentative bacterium, Pseudomonas aeruginosa, using intracellular carbon for denitrification and phosphorus-accumulation and relevant metabolic mechanisms.
abstract: A newly designed pilot-scale system was developed to enrich denitrifying phosphate-accumulating organisms (DNPAOs) for nitrogen and phosphorus nutrient removal synchronously. A strain of DNPAOs was isolated and its biochemical characteristics and metabolic mechanisms of this bacterial strain were analyzed. The results showed that compared with previously reported system, this newly designed system has higher removal rates of nutrients. Removal efficiencies of NH3-N, TN, TP, and COD in actual wastewater were 82.64%, 79.62%, 87.22%, and 90.41%, respectively. Metabolic activity of DNPAOs after anoxic stage in this study even reached 94.64%. Pseudomonas aeruginosa is a strain of non-fermentative DNPAOs with strong nitrogen and phosphorus removal abilities. Study on the metabolic mechanisms suggested that intracellular PHB of P. aeruginosa plays dual roles, supplying energy for phosphorus accumulation and serving as a major carbon source for denitrification.
authors.affiliation: Department of Environment Science and Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China; School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China; Key Laboratory of Beijing Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Environment Science and Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Environment Science and Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
authors.forename: Yanfu
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Environment Science and Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
authors.forename: Kangqun
authors.initials: K
authors.lastname: Zhou
authors.affiliation: Department of Environment Science and Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Environment Science and Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China. Electronic address: luxxx828@umn.edu.
authors.forename: Qian
authors.initials: Q
authors.lastname: Lu
authors.affiliation: Department of Environment Science and Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
authors.forename: Caibing
authors.initials: C
authors.lastname: Ming
authors.affiliation: Department of Environment Science and Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
authors.forename: Xidan
authors.initials: X
authors.lastname: Feng
authors.affiliation: Department of Environment Science and Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Du
authors.affiliation: School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Xiaoshan
authors.initials: X
authors.lastname: Jia
authors.affiliation: Key Laboratory of Beijing Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
mesh_terms: D019149:Bioreactors / Q000382:microbiology; D002244:Carbon / Q000032:analysis / Q000378:metabolism*; D058440:Denitrification; D009584:Nitrogen / Q000032:analysis / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification / Q000378:metabolism*; D062065:Wastewater / Q000737:chemistry; D018508:Water Purification / Q000379:methods
chemical_list: D002244:Carbon; D009584:Nitrogen; D010710:Phosphates; D010758:Phosphorus; D062065:Wastewater
keywords: Denitrifying phosphate-accumulating organisms; Identification; Intracellular carbon; Metabolism; Pseudomonas aeruginosa