title: Lipocalin 2 produces insulin resistance and can be upregulated by glucocorticoids in human adipose tissue.
abstract: The adipokine lipocalin 2 is linked to obesity and metabolic disorders. However, its role in human adipose tissue glucose and lipid metabolism is not explored. Here we show that the synthetic glucocorticoid dexamethasone dose-dependently increased lipocalin 2 gene expression in subcutaneous and omental adipose tissue from pre-menopausal females, while it had no effect in post-menopausal females or in males. Subcutaneous adipose tissue from both genders treated with recombinant human lipocalin 2 showed a reduction in protein levels of GLUT1 and GLUT4 and in glucose uptake in isolated adipocytes. In subcutaneous adipose tissue, lipocalin 2 increased IL-6 gene expression whereas expression of PPARγ and adiponectin was reduced. Our findings suggest that lipocalin 2 can contribute to insulin resistance in human adipose tissue. In pre-menopausal females, it may partly mediate adverse metabolic effects exerted by glucocorticoid excess.
authors.affiliation: Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
authors.forename: Prasad G
authors.initials: PG
authors.lastname: Kamble
authors.affiliation: Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Pereira
authors.affiliation: Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
authors.forename: Cherno O
authors.initials: CO
authors.lastname: Sidibeh
authors.affiliation: Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
authors.forename: Sam
authors.initials: S
authors.lastname: Amini
authors.affiliation: Department of Surgery, Uppsala University, Uppsala, Sweden.
authors.forename: Magnus
authors.initials: M
authors.lastname: Sundbom
authors.affiliation: Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
authors.forename: Joey Lau
authors.initials: JL
authors.lastname: Börjesson
authors.affiliation: Department of Medical Sciences, Uppsala University, Uppsala, Sweden. Electronic address: jan.eriksson@medsci.uu.se.
authors.forename: Jan W
authors.initials: JW
authors.lastname: Eriksson
mesh_terms: D052242:Adiponectin / Q000235:genetics; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000328:Adult; D000368:Aged; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D005938:Glucocorticoids / Q000138:chemical synthesis / Q000502:physiology*; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007333:Insulin Resistance; D000071068:Lipocalin-2 / Q000378:metabolism / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D047495:PPAR gamma / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D050151:Subcutaneous Fat / Q000187:drug effects / Q000378:metabolism; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D052242:Adiponectin; D005938:Glucocorticoids; D000071068:Lipocalin-2; D047495:PPAR gamma; D011994:Recombinant Proteins; D003907:Dexamethasone; D005947:Glucose
keywords: Glucocorticoids; Human adipose tissue; Insulin resistance; Lipocalin 2; PPARγ; Type 2 diabetes