title: Transcription Factors Expressed in Mouse Cochlear Inner and Outer Hair Cells.
abstract: Regulation of gene expression is essential to determining the functional complexity and morphological diversity seen among different cells. Transcriptional regulation is a crucial step in gene expression regulation because the genetic information is directly read from DNA by sequence-specific transcription factors (TFs). Although several mouse TF databases created from genome sequences and transcriptomes are available, a cell type-specific TF database from any normal cell populations is still lacking. We identify cell type-specific TF genes expressed in cochlear inner hair cells (IHCs) and outer hair cells (OHCs) using hair cell-specific transcriptomes from adult mice. IHCs and OHCs are the two types of sensory receptor cells in the mammalian cochlea. We show that 1,563 and 1,616 TF genes are respectively expressed in IHCs and OHCs among 2,230 putative mouse TF genes. While 1,536 are commonly expressed in both populations, 73 genes are differentially expressed (with at least a twofold difference) in IHCs and 13 are differentially expressed in OHCs. Our datasets represent the first cell type-specific TF databases for two populations of sensory receptor cells and are key informational resources for understanding the molecular mechanism underlying the biological properties and phenotypical differences of these cells.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China.|Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE, 68178, United States of America.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE, 68178, United States of America.
authors.forename: Huizhan
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE, 68178, United States of America.
authors.forename: Cody L
authors.initials: CL
authors.lastname: Barta
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, University of Nebraska Medical Center, Omaha, NE, 68198, United States of America.
authors.forename: Paul D
authors.initials: PD
authors.lastname: Judge
authors.affiliation: Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE, 68178, United States of America.|Department of Otorhinolaryngology-Head and Neck Surgery, Chinese PLA General Hospital, Beijing, 100853, China.
authors.forename: Lidong
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Pathophysiology, Second Military Medical University, Shanghai, 200433, China.
authors.forename: Weiping J
authors.initials: WJ
authors.lastname: Zhang
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Beijing Friendship Hospital, Beijing, China.
authors.forename: Shusheng
authors.initials: S
authors.lastname: Gong
authors.affiliation: Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE, 68178, United States of America.
authors.forename: Kirk W
authors.initials: KW
authors.lastname: Beisel
authors.affiliation: Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE, 68178, United States of America.
authors.forename: David Z Z
authors.initials: DZ
authors.lastname: He
mesh_terms: D000818:Animals; D015536:Down-Regulation / Q000235:genetics; D006199:Hair Cells, Auditory, Inner / Q000378:metabolism*; D018072:Hair Cells, Auditory, Outer / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D014157:Transcription Factors