title: Altered bioenergetics and developmental effects in striped marsh frog (Limnodynastes peronii) tadpoles exposed to UV treated sewage.
abstract: Effectively treating domestic wastewater so that it can be safely discharged or reused is critical for maintaining the integrity of freshwater resources, and for protecting the health of animals that rely on these systems. Amphibians are currently facing widespread population declines, so there is a particularly urgent need to investigate exposure scenarios that might result in weakened amphibian populations. Domestic sewage has received little attention as a possible factor that could influence the survival, growth and development, or general health of amphibians. However, wastewater reuse for crop irrigation and other purposes is increasing and holding ponds and constructed wetlands exist at many wastewater treatment facilities, introducing conceivable pathways that could result in the exposure of amphibians to treated wastewater. We exposed developing striped marsh frog (Limnodynastes peronii) tadpoles, to control water and 12.5, 25, 50 and 100% UV treated domestic sewage, and quantified effects on growth and development, hepatic energy reserves, and enzymatic pathways associated with detoxification and oxidative stress. Growth and development were accelerated and relative liver size was increased in exposed animals. The exposure resulted in an apparently hormetic increase in hepatic triglycerides and dose-dependent reduction in glycogen stores, as well as increased lipase and NADPH activity, indicating a general disruption to energy metabolism and/or mobilization. Contrary to expectations based on published studies with fish, we found no evidence of lipid peroxidation or induction of the detoxification enzyme Superoxide Dismutase (SOD), however, this may reflect the use of UV treatment as opposed to chlorination for disinfection. Chemical analysis and risk-based prioritization consistently identified fluoxetine, triclosan and diazinon as high-risk contaminants in the wastewater, with nonylphenol and mestranol flagged as risks during one early collection. Research is needed to explore the potential for these specific contaminants to elicit the responses identified in the present study, and to perform similar assessments using wastewater from other locations with different treatment options.
authors.affiliation: Australian Rivers Institute, Griffith School of Environment, Griffith University, Southport, QLD 4222, Australia; School of Medical and Applied Sciences, Central Queensland University, Gladstone, QLD 4702, Australia. Electronic address: s.melvin@griffith.edu.au.
authors.forename: Steven D
authors.initials: SD
authors.lastname: Melvin
authors.affiliation: Australian Rivers Institute, Griffith School of Environment, Griffith University, Southport, QLD 4222, Australia; School of Medical and Applied Sciences, Central Queensland University, Gladstone, QLD 4702, Australia.
authors.forename: Chantal M
authors.initials: CM
authors.lastname: Lanctôt
authors.affiliation: Australian Rivers Institute, Griffith School of Environment, Griffith University, Southport, QLD 4222, Australia.
authors.forename: Jason P
authors.initials: JP
authors.lastname: van de Merwe
authors.affiliation: Australian Rivers Institute, Griffith School of Environment, Griffith University, Southport, QLD 4222, Australia.
authors.forename: Frederic D L
authors.initials: FD
authors.lastname: Leusch
mesh_terms: D000818:Animals; D001001:Anura / Q000502:physiology*; D004734:Energy Metabolism / Q000502:physiology*; D016166:Free Radical Scavengers / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D007814:Larva / Q000187:drug effects* / Q000254:growth & development; D008049:Lipase / Q000378:metabolism; D008099:Liver / Q000254:growth & development / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology; D011898:Ranidae / Q000378:metabolism / Q000502:physiology*; D012722:Sewage / Q000009:adverse effects* / Q000737:chemistry; D014280:Triglycerides / Q000378:metabolism; D014466:Ultraviolet Rays; D062065:Wastewater / Q000737:chemistry / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*; D018508:Water Purification / Q000379:methods*; D053833:Wetlands
chemical_list: D016166:Free Radical Scavengers; D006003:Glycogen; D008049:Lipase; D009249:NADP; D012722:Sewage; D014280:Triglycerides; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Amphibian; Development; Energy; Glycogen; Lipase; Metabolic disruption; Sewage; Toxicity; Triglycerides; Wastewater