title: Effect of antibiotics on extracellular protein level in Pseudomonas aeruginosa.
abstract: Pseudomonas aeruginosa PAO1 organisms harbouring different plasmids were cultured in broths containing appropriate antibiotic(s). Extracellular proteins were more abundant in the presence of tetracycline or kanamycin than in the presence of other antibiotics. Zymography revealed that alkaline protease (AprA) production was interfered by these antibiotics. Extracellular proteins were not observed at the same level when AprA-deficient EG03 strains were cultured in the presence of different antibiotics. The extracellular protein levels were dependent on the antibiotics and plasmid derivative groups. Levels of extracellular protein were not significantly different between PAO1 (pBBR1MCS-5) and EG03 (pAprcomp-MCS5), and profiles of the extracellular proteome were comparable. In contrast, the level of EG03 (pBBR1MCS-MCS5) extracellular protein was higher than those observed in the other two strains. These results suggested that although AprA partially contributes to the alteration of extracellular protein level, the effect is limited.
authors.affiliation: Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan; Laboratory of Insect Genome Science, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan.
authors.forename: Eigo
authors.initials: E
authors.lastname: Takahashi
authors.affiliation: Laboratory of Insect Genome Science, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan.
authors.forename: Jae Man
authors.initials: JM
authors.lastname: Lee
authors.affiliation: Laboratory of Insect Genome Science, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Mon
authors.affiliation: Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan.
authors.forename: Yuuka
authors.initials: Y
authors.lastname: Chieda
authors.affiliation: Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan.
authors.forename: Chisa
authors.initials: C
authors.lastname: Yasunaga-Aoki
authors.affiliation: Laboratory of Insect Genome Science, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kusakabe
authors.affiliation: Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan. Electronic address: iiyama@grt.kyushu-u.ac.jp.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Iiyama
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D010450:Endopeptidases / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D007612:Kanamycin / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics*; D013752:Tetracycline / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D007612:Kanamycin; D010450:Endopeptidases; C087822:alkaline protease; D013752:Tetracycline
keywords: Alkaline protease; Extracellular protein; Kanamycin; Pseudomonas aeruginosa; Tetracycline