title: High-speed detection of DNA translocation in nanopipettes.
abstract: We present a high-speed electrical detection scheme based on a custom-designed CMOS amplifier which allows the analysis of DNA translocation in glass nanopipettes on a microsecond timescale. Translocation of different DNA lengths in KCl electrolyte provides a scaling factor of the DNA translocation time equal to p = 1.22, which is different from values observed previously with nanopipettes in LiCl electrolyte or with nanopores. Based on a theoretical model involving electrophoresis, hydrodynamics and surface friction, we show that the experimentally observed range of p-values may be the result of, or at least be affected by DNA adsorption and friction between the DNA and the substrate surface.
authors.affiliation: Imperial College London, Department of Chemistry, Exhibition Road, London SW7 2AZ, UK. t.albrecht@imperial.ac.uk.
authors.forename: Raquel L
authors.initials: RL
authors.lastname: Fraccari
authors.affiliation: Politecnico di Milano, Dipartimento di Elettronica, Informazione e Bioingegneria, P.za Leonardo da Vinci 32, Milano, Italy.
authors.forename: Pietro
authors.initials: P
authors.lastname: Ciccarella
authors.affiliation: Imperial College London, Department of Chemistry, Exhibition Road, London SW7 2AZ, UK. t.albrecht@imperial.ac.uk.
authors.forename: Azadeh
authors.initials: A
authors.lastname: Bahrami
authors.affiliation: Politecnico di Milano, Dipartimento di Elettronica, Informazione e Bioingegneria, P.za Leonardo da Vinci 32, Milano, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Carminati
authors.affiliation: Politecnico di Milano, Dipartimento di Elettronica, Informazione e Bioingegneria, P.za Leonardo da Vinci 32, Milano, Italy.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Ferrari
authors.affiliation: Imperial College London, Department of Chemistry, Exhibition Road, London SW7 2AZ, UK. t.albrecht@imperial.ac.uk.
authors.forename: Tim
authors.initials: T
authors.lastname: Albrecht
mesh_terms: D004247:DNA / Q000032:analysis / Q000737:chemistry; D018021:Lithium Chloride / Q000737:chemistry; D008956:Models, Chemical; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D011189:Potassium Chloride / Q000737:chemistry
chemical_list: D011189:Potassium Chloride; D004247:DNA; D018021:Lithium Chloride