title: B4GALT3 up-regulation by miR-27a contributes to the oncogenic activity in human cervical cancer cells.
abstract: β-1,4-Galactosyltransferase III (B4GALT3) is an enzyme responsible for the generation of poly-N-acetyllactosamine and is involved in tumorigenesis. However, B4GALT3-dysregulation and its role in cervical cancer cells are unknown. Herein, we found that B4GALT3 was upregulated in cervical cancer tissues compared to adjacent non-tumor tissues. B4GALT3-overexpression promoted, whereas B4GALT3-knockdown suppressed the cellular migration, invasion and EMT of HeLa and C33A cervical cancer cells. To explore the mechanism of dysregulation, B4GALT3 was predicted to be a target of miR-27a. EGFP and pGL3-promoter reporter assay showed miR-27a binds to B4GALT3 3'UTR region but enhanced its expression. RT-qPCR showed miR-27a was also upregulated and presented positive correlation with B4GALT3-expression in cervical cancer tissues. miR-27a-overexpression promoted, but blocking-miR-27a repressed these malignancies in HeLa and C33A cells. Furthermore, shR-B4GALT3 counteracted the promotion of malignancies induced by miR-27a, suggesting miR-27a upregulates B4GALT3 to enhance tumorigenic activities. In addition, we found that B4GALT3 significantly enhances β1-integrin stability, thus mediating promotion of B4GALT3 on malignancy in cervical cancer cells. Altogether, our findings evidenced that B4GALT3 upregulated by miR-27a contributes to the tumorigenic activities by β1-integrin pathway and might provide potential biomarkers for cervical cancer.
authors.affiliation: Tianjin Life Science Research Center and School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Yanrui
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Tianjin Life Science Research Center and School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Yang
authors.affiliation: Tianjin Life Science Research Center and School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: Tianjin Life Science Research Center and School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China. Electronic address: htang2002@yahoo.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Tang
mesh_terms: D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D005260:Female; D005700:Galactosyltransferases / Q000096:biosynthesis*; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D019012:Integrin beta1 / Q000235:genetics; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D019012:Integrin beta1; C531388:MIRN27 microRNA, human; D035683:MicroRNAs; D005700:Galactosyltransferases; C427753:beta4-galactosyltransferase III
keywords: B4GALT3; Cervical cancer cells; EMT; miR-27a; β1-integrin