title: Toxicity analysis of various Pluronic F-68-coated carbon nanotubes on mesenchymal stem cells.
abstract: Carbon nanotubes (CNTs) have poor colloid stability in biological media and exert cytotoxic effects on mesenchymal stem cells (MSCs). Modification with polymeric surfactant is a widely used strategy to enhance water dispersibility of CNTs. This study investigated the toxic effects of various Pluronic F-68 (PF68)-coated multi-walled CNTs (MWCNTs) on rat bone marrow-derived MSCs.PF68-coated MWCNTs showed favorable biocompatibility to MSCs that the cell viability, apoptosis, and reactive oxygen species (ROS) were not altered after 24 h of co-incubation. Nevertheless, significant apoptosis induction and massive ROS release were found following extended exposure (48 and 72 h), and the toxic impact was dependent on the initial surface properties of the encapsulated MWCNTs. All the types of PF68-coated MWCNTs did not affect the cell-surface markers and in vivo biodistribution of MSCs. Our results suggest that proper polymer coating can reduce the acute toxicity of MWCNTs to MSCs but without altering their biological fate.
authors.affiliation: Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, Zhejiang, PR China.
authors.forename: Meng-Zhu
authors.initials: MZ
authors.lastname: Yao
authors.affiliation: Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, Zhejiang, PR China.
authors.forename: Yu-Lan
authors.initials: YL
authors.lastname: Hu
authors.affiliation: Hangzhou SoliPharma Co., Ltd, 6th Street Hangzhou Economic and Technological Development Zone, Hangzhou 310058, Zhejiang, PR China.
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Sheng
authors.affiliation: First Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310058, Zhejiang, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lin
authors.affiliation: Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, Zhejiang, PR China. Electronic address: lingds@zju.edu.cn.
authors.forename: Daishun
authors.initials: D
authors.lastname: Ling
authors.affiliation: Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, Zhejiang, PR China. Electronic address: gaojianqing@zju.edu.cn.
authors.forename: Jian-Qing
authors.initials: JQ
authors.lastname: Gao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D008297:Male; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D037742:Nanotubes, Carbon / Q000032:analysis / Q000737:chemistry / Q000633:toxicity* / Q000648:ultrastructure; D020442:Poloxamer / Q000737:chemistry / Q000493:pharmacokinetics / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D013499:Surface Properties; D014018:Tissue Distribution
chemical_list: D037742:Nanotubes, Carbon; D017382:Reactive Oxygen Species; D020442:Poloxamer
keywords: Biodistribution; Chemical functionalized carbon nanotubes; Cytotoxicity; Mesenchymal stem cells; Pluronic F-68