title: Selective ciprofloxacin antibiotic detection by fluorescent siderophore pyoverdin.
abstract: Fluorescent siderophore pyoverdin (PVD) was produced from a soil isolate Pseudomonas monteilii strain MKP 213. The PVD was purified near to homogeneity and applied for the fluorescent chemosensing of various antibiotics in aqueous solution (pH=7.0). Upon addition of ciprofloxacin, PVD showed new UV-vis absorption bands at 252 and 321nm due to an internal charge transfer mechanism. Also, the addition of ciprofloxacin induced a highly selective fluorescence enhancement of PVD with a 13nm blue shift from 458 to 445nm. The combination of a long peptide chain along with the chromophore unit of PVD generates a converging cleft for ciprofloxacin recognition with LOD and LOQ of 7.13μM and 21.6μM, respectively without interference from other studied antibiotics. The association constant (Ka) of PVD with ciprofloxacin was calculated to be as low as 1.40×10(5)M(-1) using Benesi-Hildebrand plot depicting its significance in detection. The pharmaceutical tablet analysis measures the sensing with negligible matrix effect and quantitative recovery.
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, 425001 Maharashtra, India.
authors.forename: Madhuri K
authors.initials: MK
authors.lastname: Pawar
authors.affiliation: School of Chemical Sciences, North Maharashtra University, Jalgaon, 425001 Maharashtra, India.
authors.forename: Kundan C
authors.initials: KC
authors.lastname: Tayade
authors.affiliation: Department of Applied Chemistry, SV National Institute of Technology, Surat, 395007 Gujarat, India.
authors.forename: Suban K
authors.initials: SK
authors.lastname: Sahoo
authors.affiliation: School of Chemical Sciences, North Maharashtra University, Jalgaon, 425001 Maharashtra, India.
authors.forename: Pramod P
authors.initials: PP
authors.lastname: Mahulikar
authors.affiliation: School of Chemical Sciences, North Maharashtra University, Jalgaon, 425001 Maharashtra, India. Electronic address: kuwaras@gmail.com.
authors.forename: Anil S
authors.initials: AS
authors.lastname: Kuwar
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, 425001 Maharashtra, India. Electronic address: blchaudhari@hotmail.com.
authors.forename: Bhushan L
authors.initials: BL
authors.lastname: Chaudhari
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D002939:Ciprofloxacin / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D061986:MCF-7 Cells; D009842:Oligopeptides / Q000737:chemistry*; D011549:Pseudomonas / Q000737:chemistry; D017262:Siderophores / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D000900:Anti-Bacterial Agents; D005456:Fluorescent Dyes; D009842:Oligopeptides; D017262:Siderophores; D002939:Ciprofloxacin; C042453:pyoverdin
keywords: Antibiotics sensing; Ciprofloxacin; Fluorescent siderophore; Pyoverdin