title: Intraluminal Drug Delivery to the Mouse Arteriovenous Fistula Endothelium.
abstract: Delivery of therapeutic agents to enhance arteriovenous fistula (AVF) maturation can be administered either via intraluminal or external routes. The simple murine AVF model was combined with intraluminal administration of drug solution to the venous endothelium at the same time as fistula creation. Technical aspects of this model are discussed. Under general anesthesia, an abdominal incision is made and the aorta and inferior vena cava (IVC) are exposed. The infra-renal aorta and IVC are dissected for clamping. After proximal and distal clamping, the puncture site is exposed and a 25 G needle is used to puncture both walls of the aorta and into the IVC. Immediately after the puncture, a reporter gene-expressing viral vector was infused in the IVC via the same needle, followed by 15 min of incubation. The intraluminal administration method enabled more robust viral gene delivery to the venous endothelium compared to administration by the external route. This novel method of delivery will facilitate studies that explore the role of the endothelium in AVF maturation and enable intraluminal drug delivery at the time of surgical operation.
authors.affiliation: Department of Surgery and the Vascular Biology and Therapeutics Program, Yale University; Department of Vascular Surgery, University of Tokyo; Department of Vascular Surgery, VA Connecticut Healthcare Systems.
authors.forename: Takuya
authors.initials: T
authors.lastname: Hashimoto
authors.affiliation: Department of Surgery and the Vascular Biology and Therapeutics Program, Yale University; Department of Vascular Surgery, University of Tokyo; Department of Vascular Surgery, VA Connecticut Healthcare Systems.
authors.forename: Kota
authors.initials: K
authors.lastname: Yamamoto
authors.affiliation: Department of Surgery and the Vascular Biology and Therapeutics Program, Yale University.
authors.forename: Trenton
authors.initials: T
authors.lastname: Foster
authors.affiliation: Department of Surgery and the Vascular Biology and Therapeutics Program, Yale University.
authors.forename: Hualong
authors.initials: H
authors.lastname: Bai
authors.affiliation: Department of Vascular Surgery, International University of Health and Welfare Mita Hospital.
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Shigematsu
authors.affiliation: Department of Surgery and the Vascular Biology and Therapeutics Program, Yale University; Department of Vascular Surgery, VA Connecticut Healthcare Systems; alan.dardik@yale.edu.
authors.forename: Alan
authors.initials: A
authors.lastname: Dardik
mesh_terms: D000818:Animals; D001011:Aorta; D001164:Arteriovenous Fistula; D004195:Disease Models, Animal; D016503:Drug Delivery Systems / Q000379:methods*; D004727:Endothelium / Q000187:drug effects; D051379:Mice; D014682:Vena Cava, Inferior / Q000601:surgery