title: A SNP-Based Molecular Barcode for Characterization of Common Wheat.
abstract: Wheat is grown as a staple crop worldwide. It is important to develop an effective genotyping tool for this cereal grain both to identify germplasm diversity and to protect the rights of breeders. Single-nucleotide polymorphism (SNP) genotyping provides a means for developing a practical, rapid, inexpensive and high-throughput assay. Here, we investigated SNPs as robust markers of genetic variation for typing wheat cultivars. We identified SNPs from an array of 9000 across a collection of 429 well-known wheat cultivars grown in China, of which 43 SNP markers with high minor allele frequency and variations discriminated the selected wheat varieties and their wild ancestors. This SNP-based barcode will allow for the rapid and precise identification of wheat germplasm resources and newly released varieties and will further assist in the wheat breeding program.
authors.affiliation: Key Laboratory of Crop Gene Resources and Germplasm Enhancement, MOA, the National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, CAAS, Beijing, 100081, China.
authors.forename: LiFeng
authors.initials: L
authors.lastname: Gao
authors.affiliation: Key Laboratory of Crop Gene Resources and Germplasm Enhancement, MOA, the National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, CAAS, Beijing, 100081, China.
authors.forename: JiZeng
authors.initials: J
authors.lastname: Jia
authors.affiliation: Key Laboratory of Crop Gene Resources and Germplasm Enhancement, MOA, the National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, CAAS, Beijing, 100081, China.
authors.forename: XiuYing
authors.initials: X
authors.lastname: Kong
mesh_terms: D005787:Gene Frequency; D014644:Genetic Variation; D018745:Genome, Plant; D005838:Genotype; D000069600:Plant Breeding; D020641:Polymorphism, Single Nucleotide; D014908:Triticum / Q000235:genetics*