title: Immobilization of Bacillus subtilis lipase on a Cu-BTC based hierarchically porous metal-organic framework material: a biocatalyst for esterification.
abstract: Bacillus subtilis lipase (BSL2) has been successfully immobilized into a Cu-BTC based hierarchically porous metal-organic framework material for the first time. The Cu-BTC hierarchically porous MOF material with large mesopore apertures is prepared conveniently by using a template-free strategy under mild conditions. The immobilized BSL2 presents high enzymatic activity and perfect reusability during the esterification reaction. After 10 cycles, the immobilized BSL2 still exhibits 90.7% of its initial enzymatic activity and 99.6% of its initial conversion.
authors.affiliation: State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun, 130012, P. R. China. yunling@jlu.edu.cn.
authors.forename: Yu
authors.initials: Y
authors.lastname: Cao
authors.forename: Zhuofu
authors.initials: Z
authors.lastname: Wu
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiao
authors.forename: Qisheng
authors.initials: Q
authors.lastname: Huo
authors.forename: Yunling
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000327:Adsorption; D001412:Bacillus subtilis / Q000201:enzymology*; D055162:Biocatalysis; D003300:Copper / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D004951:Esterification; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D008968:Molecular Conformation; D009942:Organometallic Compounds / Q000737:chemistry*; D016062:Porosity
chemical_list: D004800:Enzymes, Immobilized; D009942:Organometallic Compounds; D003300:Copper; D008049:Lipase