title: Genetic Diversity of NHE1, Receptor for Subgroup J Avian Leukosis Virus, in Domestic Chicken and Wild Anseriform Species.
abstract: J subgroup avian leukosis virus (ALV-J) infects domestic chicken, jungle fowl, and turkey and enters the host cell through a receptor encoded by tvj locus and identified as Na+/H+ exchanger 1 (NHE1). The resistance to ALV-J in a great majority of examined galliform species was explained by deletions or substitutions of the critical tryptophan 38 in the first extracellular loop of NHE1, and genetic polymorphisms around this site predict the susceptibility or resistance of a given species or individual. In this study, we examined the NHE1 polymorphism in domestic chicken breeds and documented quantitative differences in their susceptibility to ALV-J in vitro. In a panel of chicken breeds assembled with the aim to cover the maximum variability encountered in domestic chickens, we found a completely uniform sequence of NHE1 extracellular loop 1 (ECL1) without any source of genetic variation for the selection of ALV-J-resistant poultry. In parallel, we studied the natural polymorphisms of NHE1 in wild ducks and geese because of recent reports on ALV-J positivity in feral Asian species. In anseriform species, we demonstrate a specific and highly conserved critical ECL1 sequence without any homologue of tryptophan 38 in accordance with the resistance of duck cells to prototype ALV-J. Last, we demonstrated that the new Asian strains of ALV-J have not evolved their envelope glycoprotein to the entry the duck cells. Our results contribute substantially to the current discussion of possible heterotransmission of ALV-J and its spill-over into the wild ducks and geese.
authors.affiliation: Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, CZ-14220, Prague 4, Czech Republic.
authors.forename: Markéta
authors.initials: M
authors.lastname: Reinišová
authors.affiliation: Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, CZ-14220, Prague 4, Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Plachý
authors.affiliation: Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, CZ-14220, Prague 4, Czech Republic.
authors.forename: Dana
authors.initials: D
authors.lastname: Kučerová
authors.affiliation: Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, CZ-14220, Prague 4, Czech Republic.
authors.forename: Filip
authors.initials: F
authors.lastname: Šenigl
authors.affiliation: Charles University in Prague, Faculty of Science, Department of Zoology, Vinicna 7, CZ-12844, Prague 2, Czech Republic.
authors.forename: Michal
authors.initials: M
authors.lastname: Vinkler
authors.affiliation: Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, CZ-14220, Prague 4, Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Hejnar
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000829:Animals, Domestic; D000835:Animals, Wild; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D014644:Genetic Variation; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D017923:Sodium-Hydrogen Exchangers / Q000737:chemistry / Q000235:genetics*
chemical_list: D017923:Sodium-Hydrogen Exchangers