title: Influence of an Enterococcus faecium probiotic on the development of Peyer's patches B cells in piglets.
abstract: To study the interactions between a probiotic bacterium and the host's immune system, we undertook a feeding trial with Enterococcus faecium SF68 (NCIMB 10415). Starting at an age of 1 day, piglets received a daily oral dose of the probiotic bacteria. Immune cells were isolated from the blood and the distal continuous Peyer's patch (PP) of the piglets. While the percentage of B cells in the distal continuous PP was not influenced by the probiotic treatment, an elevated expression of CD1 on Peyer's patch B cells was observed after probiotic treatment. Furthermore, the fraction of CD4-CD8+ cells was decreased in this organ. In blood lymphocyte fractions of the probiotic-treated piglets, the proportion of CD16-positive cells was also diminished, whereas the portion of γδ T cells and CD4-positive T cells increased. The data indicate that early administration of the probiotic Enterococcus faecium can modulate the composition of blood lymphocyte populations in piglets.
authors.affiliation: a Institut für Immunologie und Molekularbiologie, Freie Universität Berlin.
authors.forename: Lydia
authors.initials: L
authors.lastname: Scharek-Tedin
authors.affiliation: b Charité-Universitätsmedizin Berlin, Campus Benjamin Franklin, Institut für Molekularbiologie und Bioinformatik , Berlin.
authors.forename: Matthias
authors.initials: M
authors.lastname: Filter
authors.affiliation: c Institut für Tierernährung, Freie Universität Berlin , Germany.
authors.forename: David
authors.initials: D
authors.lastname: Taras
authors.affiliation: b Charité-Universitätsmedizin Berlin, Campus Benjamin Franklin, Institut für Molekularbiologie und Bioinformatik , Berlin.
authors.forename: Paul
authors.initials: P
authors.lastname: Wrede
authors.affiliation: a Institut für Immunologie und Molekularbiologie, Freie Universität Berlin.
authors.forename: Michael F G
authors.initials: MF
authors.lastname: Schmidt
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D018949:Antigens, CD1 / Q000378:metabolism*; D001402:B-Lymphocytes / Q000378:metabolism*; D016984:Enterococcus faecium / Q000737:chemistry*; D007082:Ileum / Q000187:drug effects / Q000378:metabolism; D010581:Peyer's Patches / Q000254:growth & development*; D019936:Probiotics / Q000008:administration & dosage; D034421:Sus scrofa / Q000378:metabolism*
chemical_list: D018949:Antigens, CD1
keywords: B lymphocytes; CD1 cells; Enterococcus faecium; Peyer's patches; piglets; probiotics