title: Mutations in lysX as the new and reliable markers for tuberculosis Beijing and modern Beijing strains.
abstract: Genotyping results and DNA sequencing analysis of 235 Mycobacterium tuberculosis (M. tuberculosis) isolates from China indicated that mutations at codon 995 (Pro CCG to Pro CCA) and 701 (Ile ATT to Thr ACT) in lysX gene (Rv1640c), are specific markers for Beijing and modern Beijing strains, respectively. This observation was also confirmed by 24 genomes of M. tuberculosis strains from other countries. Moreover, a simple and fast multiplex allele-specific PCR (MAS-PCR) method for detecting mutations at codon 995 and 701 in lysX has been established and used to screen 235 DNA samples obtained from M. tuberculosis isolates. In all cases, Beijing and modern Beijing strains were identified correctly.
authors.affiliation: State Key Laboratory for Infectious Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Li-li
authors.initials: LL
authors.lastname: Zhao
authors.affiliation: State Key Laboratory for Infectious Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Tong-yang
authors.initials: TY
authors.lastname: Xiao
authors.affiliation: State Key Laboratory for Infectious Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Department of Medicine, Hunan Traditional Chinese Medical College, Zhuzhou 412012, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Sun
authors.affiliation: State Key Laboratory for Infectious Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Hai-can
authors.initials: HC
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Infectious Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Xiu-qin
authors.initials: XQ
authors.lastname: Zhao
authors.affiliation: State Key Laboratory for Infectious Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: State Key Laboratory for Infectious Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Gui-lian
authors.initials: GL
authors.lastname: Li
authors.affiliation: Hulunbeier People's Hospital, Hulunbeier 021000, China.
authors.forename: Chun-yan
authors.initials: CY
authors.lastname: Zeng
authors.affiliation: State Key Laboratory for Infectious Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Department of Medicine, Hunan Traditional Chinese Medical College, Zhuzhou 412012, China. Electronic address: wankanglin@icdc.cn.
authors.forename: Kang-lin
authors.initials: KL
authors.lastname: Wan
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001431:Bacteriological Techniques; D002681:China; D003062:Codon; D004252:DNA Mutational Analysis; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D008250:Lysine-tRNA Ligase / Q000235:genetics*; D060885:Multiplex Polymerase Chain Reaction; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000472:pathogenicity; D010641:Phenotype; D011237:Predictive Value of Tests; D011487:Protein Conformation; D055550:Protein Stability; D015203:Reproducibility of Results; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D003062:Codon; D005819:Genetic Markers; D008250:Lysine-tRNA Ligase; C000612992:lysX protein, Mycobacterium tuberculosis
keywords: Beijing genotype; Genotype; Marker; Modern Beijing subtype; Multiplex allele-specific PCR; Mycobacterium tuberculosis