title: Discriminative power of Campylobacter phenotypic and genotypic typing methods.
abstract: The aim of this study was to compare different typing methods, individually and combined, for use in the monitoring of Campylobacter in food. Campylobacter jejuni (n=94) and Campylobacter coli (n=52) isolated from different broiler meat carcasses were characterized using multilocus sequence typing (MLST), flagellin gene A restriction fragment length polymorphism typing (flaA-RFLP), antimicrobial resistance profiling (AMRp), the presence/absence of 5 putative virulence genes; and, exclusively for C. jejuni, the determination of lipooligosaccharide (LOS) class. Discriminatory power was calculated by the Simpson's index of diversity (SID) and the congruence was measured by the adjusted Rand index and adjusted Wallace coefficient. MLST was individually the most discriminative typing method for both C. jejuni (SID=0.981) and C. coli (SID=0.957). The most discriminative combination with a SID of 0.992 for both C. jejuni and C. coli was obtained by combining MLST with flaA-RFLP. The combination of MLST with flaA-RFLP is an easy and feasible typing method for short-term monitoring of Campylobacter in broiler meat carcass.
authors.affiliation: Laboratory of Food Microbiology and Food Preservation, Dept. Food Safety & Food Quality Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Gent, Belgium; Institute of Public Health, Scientific service Food-borne pathogens, Juliette Wytsman Street 14, 1050 Brussels, Belgium.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Duarte
authors.affiliation: Laboratory of Food Microbiology and Food Preservation, Dept. Food Safety & Food Quality Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Gent, Belgium; Dept. Veterinary Public Health & Food Safety, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Seliwiorstow
authors.affiliation: USDA, ARS, WRRC, Produce Safety and Microbiology, 800 Buchanan Street, Alabany, CA, 94710, USA.
authors.forename: William G
authors.initials: WG
authors.lastname: Miller
authors.affiliation: Dept. Veterinary Public Health & Food Safety, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: Lieven
authors.initials: L
authors.lastname: De Zutter
authors.affiliation: Laboratory of Food Microbiology and Food Preservation, Dept. Food Safety & Food Quality Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Gent, Belgium.
authors.forename: Mieke
authors.initials: M
authors.lastname: Uyttendaele
authors.affiliation: Institute of Public Health, Scientific service Food-borne pathogens, Juliette Wytsman Street 14, 1050 Brussels, Belgium.
authors.forename: Katelijne
authors.initials: K
authors.lastname: Dierick
authors.affiliation: Institute of Public Health, Scientific service Food-borne pathogens, Juliette Wytsman Street 14, 1050 Brussels, Belgium.
authors.forename: Nadine
authors.initials: N
authors.lastname: Botteldoorn
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods* / Q000592:standards; D017000:Campylobacter coli / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D005408:Flagellin / Q000235:genetics; D005516:Food Microbiology / Q000379:methods*; D060005:Genotyping Techniques / Q000379:methods / Q000592:standards; D008070:Lipopolysaccharides / Q000145:classification; D008460:Meat / Q000382:microbiology*; D058885:Multilocus Sequence Typing / Q000379:methods; D010641:Phenotype; D012150:Polymorphism, Restriction Fragment Length; D011200:Poultry / Q000382:microbiology; D037521:Virulence Factors / Q000235:genetics
chemical_list: D008070:Lipopolysaccharides; D037521:Virulence Factors; C023023:lipid-linked oligosaccharides; D005408:Flagellin
keywords: Antimicrobial resistance; Broiler meat; Campylobacter coli; Campylobacter jejuni; Food; MLST; Typing methods; flaA-RFLP