title: Short communication: The effect of storage conditions over time on bovine colostral immunoglobulin G concentration, bacteria, and pH.
abstract: The objective of the present study was to measure the effect of storing colostrum in different conditions for varying amounts of time on IgG concentration, bacteria, and pH. In experiment 1, colostrum from 12 Holstein-Friesian cows (6 primiparous and 6 multiparous) was collected within 3h of calving, and colostrum from another 12 multiparous cows was collected within 3h of calving (6 cows) and >9h postpartum (6 cows). Aliquots were refrigerated or stored at room temperature for up to 72h, depending on treatment. In experiment 2, colostrum was collected from 6 multiparous cows within 9h of calving, and aliquots were stored for up to 72h in temperature-controlled units set at 4, 13, and 20°C. All colostrum samples were analyzed for IgG concentration, total bacteria count, and pH after 0, 6, 12, 24, 36, 48, 60, and 72h of storage. Storage conditions did not affect the IgG concentration of colostrum. Bacterial growth was most rapid in the first 6h of storage, reducing thereafter, but bacteria multiplied at a significantly greater rate when stored in warmer conditions (i.e., >4°C). The pH of colostrum was not significantly altered when stored at temperatures <13°C, but when stored at 20°C the pH significantly decreased after 24h of storage. Storing colostrum in warmer conditions significantly alters both total bacteria count and pH; consequently, colostrum should be stored at ≤4°C.
authors.affiliation: Teagasc, Animal and Grassland Research and Innovation Centre, Moorepark, Fermoy, Co. Cork, Ireland; School of Veterinary Medicine, University College Dublin, Dublin 4, Ireland.
authors.forename: C
authors.initials: C
authors.lastname: Cummins
authors.affiliation: School of Veterinary Medicine, University College Dublin, Dublin 4, Ireland.
authors.forename: I
authors.initials: I
authors.lastname: Lorenz
authors.affiliation: Teagasc, Animal and Grassland Research and Innovation Centre, Moorepark, Fermoy, Co. Cork, Ireland. Electronic address: Emer.Kennedy@teagasc.ie.
authors.forename: E
authors.initials: E
authors.lastname: Kennedy
mesh_terms: D000818:Animals; D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification; D002417:Cattle; D015169:Colony Count, Microbial; D003126:Colostrum / Q000737:chemistry* / Q000382:microbiology*; D005260:Female; D061353:Food Storage; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000032:analysis*; D010298:Parity; D011247:Pregnancy; D013696:Temperature
chemical_list: D007074:Immunoglobulin G
keywords: bacteria; colostrum storage; immunoglobulin G; pH