title: Purification of porcine reproductive and respiratory syndrome virus using ultrafiltration and liquid chromatography.
abstract: Porcine reproductive and respiratory syndrome (PRRS) virus causes severe and persistent disease in pigs worldwide. Its heterogeneity poses a major challenge for the effective prevention and control of PRRS. Purified viruses are essential for serological studies. Traditional methods for purifying PRRS virus are time- and labor-intensive and difficult to scale-up and requires long processing time. Here, we describe a rapid, simple, scalable process for PRRS virus purification. Highly pure viral particles were obtained after ultrafiltration and liquid chromatography, as confirmed by SDS-PAGE and electron microscopy. The overall process achieved a recovery of 50% of raw virus, with a purity close to that obtained by CsCl coupled with sucrose density gradients. The purification process described here should be useful in large-scale production of highly pure PRRS virus.
authors.affiliation: Beijing Anheal Laboratories Co., Ltd., Beijing 100094, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Bohua
authors.affiliation: Beijing Anheal Laboratories Co., Ltd., Beijing 100094, China.
authors.forename: Sun
authors.initials: S
authors.lastname: Ming
authors.affiliation: Beijing Anheal Laboratories Co., Ltd., Beijing 100094, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Beijing Anheal Laboratories Co., Ltd., Beijing 100094, China.
authors.forename: Deng
authors.initials: D
authors.lastname: Xiaoyu
authors.affiliation: Beijing Anheal Laboratories Co., Ltd., Beijing 100094, China.
authors.forename: Li
authors.initials: L
authors.lastname: Baochun
authors.affiliation: Beijing Anheal Laboratories Co., Ltd., Beijing 100094, China.
authors.forename: Shentu
authors.initials: S
authors.lastname: Fenqin
authors.affiliation: Beijing Anheal Laboratories Co., Ltd., Beijing 100094, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Beijing Anheal Laboratories Co., Ltd., Beijing 100094, China. Electronic address: chenxizhao@anheal.com.
authors.forename: Chen
authors.initials: C
authors.lastname: Xizhao
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D008854:Microscopy, Electron; D019316:Porcine respiratory and reproductive syndrome virus / Q000302:isolation & purification*; D013552:Swine; D014462:Ultrafiltration / Q000379:methods*
keywords: Chromatography; Porcine reproductive and respiratory syndrome virus; Purification