title: Determination of Candesartan in Human Plasma with Liquid Chromatography - Tandem Mass Spectrometry.
abstract: A sensitive, specific and rapid liquid chromatography - tandem mass spectrometry method was developed and validated for the determination of candesartan in human plasma. Analyte was separated from endogenous components present in plasma by solid phase extraction. Chromatographic separation was performed on Gemini C18 analytical column using mobile phase acetonitrile - 5 mM ammonium formate pH 2 (90:10, v/v) at flow rate of 0.3 mL/min. For detection, tandem mass spectrometry in SRM mode with positive electrospray ionization was used. The mass transitions m/z 441.1 > 263.1 and 445.1 > 267.1 were used to determine candesartan by using candesartan-d4 as an internal standard. After development, the method was validated according to the requirements of EMA regulatory guidelines in the concentration range 1 - 400 ng/ml in human plasma. Limit of quantification (LLOQ) was 1 ng/ml. The developed and validated method proved to be very fast and reproducible and was therefore successfully implemented in pharmacokinetic and bioequivalence studies with large number of study samples.
authors.forename: Vanja
authors.initials: V
authors.lastname: Forjan
authors.forename: Lea
authors.initials: L
authors.lastname: Cvitkovič Maričič
authors.forename: Helena
authors.initials: H
authors.lastname: Prosen
authors.forename: Darinka
authors.initials: D
authors.lastname: Brodnjak Vončina
mesh_terms: D001562:Benzimidazoles / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D001713:Biphenyl Compounds; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D012995:Solubility; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013777:Tetrazoles / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics
chemical_list: D001562:Benzimidazoles; D001713:Biphenyl Compounds; D013777:Tetrazoles; C081643:candesartan