title: Glycosidases Interact Selectively With Mannose-6-Phosphate Receptors of Bull Spermatozoa.
abstract: Glycosidases may play a role in sperm maturation during epididymal transit. In this work, we describe the interaction of these enzymes with bull spermatozoa. We found that β-galactosidase associated to spermatozoa can be released under low ionic strength conditions, whereas the interaction of N-acetyl-β-D-glucosaminidase and β-glucuronidase with spermatozoa appeared to be stronger. On the other hand, α-mannosidase and α-fucosidase cannot be removed from the gametes. In addition, part of N-acetyl-β-D-glucosaminidase, β-galactosidase, and β-glucuronidase can also be released by mannose-6-phosphate. Taking into account these data, we explored the presence of cation-independent- and cation-dependent-mannose-6-phosphate receptors in the spermatozoa and found that cation-independent mannose-6-phosphate receptor is highly expressed in bull spermatozoa and cation-dependent-mannose-6-phosphate receptor is expressed at a lesser extent. In addition, by immunofluorescence, we observed that cation-independent-mannose-6-phosphate receptor is mostly located at the acrosomal zone, whereas cation-dependent-mannose-6-phosphate receptor presents a different distribution pattern on spermatozoa during the epididymal transit. N-acetyl-β-D-glucosaminidase and β-glucuronidase isolated from epididymal fluid interacted mostly with cation-independent-mannose-6-phosphate receptor, while β-galactosidase was recognized by both receptors. We concluded that glycosidases might play different roles in bull spermatozoa and that mannos-6-phosphate receptors may act as recruiters of some enzymes. J. Cell. Biochem. 117: 2464-2472, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Laboratorio de Biología y Fisiología Celular "Dr. Franciso Bertini," Instituto de Histología y Embriología-CONICET-FCM-UNCuyo, Facultad de Ciencias Exactas y Naturales-Universidad Nacional de Cuyo, 5500, Mendoza, Argentina.
authors.forename: Andrea C
authors.initials: AC
authors.lastname: Aguilera
authors.affiliation: Laboratorio de Biología y Fisiología Celular "Dr. Franciso Bertini," Instituto de Histología y Embriología-CONICET-FCM-UNCuyo, 5500, Mendoza, Argentina.
authors.forename: Verónica
authors.initials: V
authors.lastname: Boschin
authors.affiliation: Laboratorio de Biología y Fisiología Celular "Dr. Franciso Bertini," Instituto de Histología y Embriología-CONICET-FCM-UNCuyo, Facultad de Ciencias Exactas y Naturales-Universidad Nacional de Cuyo, 5500, Mendoza, Argentina.
authors.forename: Lorena
authors.initials: L
authors.lastname: Carvelli
authors.affiliation: Laboratorio de Biología y Fisiología Celular "Dr. Franciso Bertini," Instituto de Histología y Embriología-CONICET-FCM-UNCuyo, 5500, Mendoza, Argentina.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Cavicchia
authors.affiliation: Laboratorio de Biología y Fisiología Celular "Dr. Franciso Bertini," Instituto de Histología y Embriología-CONICET-FCM-UNCuyo, Facultad de Ciencias Exactas y Naturales-Universidad Nacional de Cuyo, 5500, Mendoza, Argentina. msosa@fcm.uncu.edu.ar.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Sosa
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D004822:Epididymis / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006026:Glycoside Hydrolases / Q000378:metabolism*; D008297:Male; D008360:Mannosephosphates / Q000378:metabolism*; D017527:Receptor, IGF Type 2 / Q000378:metabolism*; D013094:Spermatozoa / Q000378:metabolism*
chemical_list: D008360:Mannosephosphates; D017527:Receptor, IGF Type 2; C034304:cation-dependent mannose-6-phosphate receptor; C027693:mannose-6-phosphate; D006026:Glycoside Hydrolases
keywords: BULL EPIDIDYMIS; LYSOSOMAL ENZYMES; MALE REPRODUCTIVE TRACT; MANNOSE-6-PHOSPHATE RECEPTORS; SPERMATOZOA