title: Asymptomatic faecal carriage of ESBL producing enterobacteriaceae in Hungarian healthy individuals and in long-term care applicants: A shift towards CTX-M producers in the community.
abstract: Background Faecal carriage of extended-spectrum beta-lactamase (ESBL) producing Enterobacteriaceae in healthy individuals was examined and compared to previous results obtained in such individuals a few years earlier. Methods Faecal samples from 779 individuals screened for employment purposes and from 225 applicants to long-term care (LTC) were screened between November 2013 and May 2014. Results The overall rate of faecal carriage was 3.0% (30/1004). The carriage rate was significantly higher in applicants for LTC (5.3% vs 2.3%; p = 0.019). All isolates carried CTX-M ESBLs, with an overwhelming dominance of blaCTX-M-15 (84.4%) in both groups and in both E. coli and Klebsiella pneumoniae. Conclusions The prevalences were comparable to those in the earlier study, but a marked decrease of the diversity of ESBL genes in E. coli from the employment screening group was found, suggesting that the ESBL-producing isolates originating from diverse sources are being replaced by highly successful blaCTX-M-15 producing strains.
authors.affiliation: a Department of Medical Microbiology , University of Debrecen , Debrecen , Hungary ;
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Ebrahimi
authors.affiliation: a Department of Medical Microbiology , University of Debrecen , Debrecen , Hungary ;
authors.forename: Julianna
authors.initials: J
authors.lastname: Mózes
authors.affiliation: b Synlab Ltd. , Microbiology Debrecen , Debrecen , Hungary.
authors.forename: Júlia
authors.initials: J
authors.lastname: Mészáros
authors.affiliation: b Synlab Ltd. , Microbiology Debrecen , Debrecen , Hungary.
authors.forename: Ágnes
authors.initials: Á
authors.lastname: Juhász
authors.affiliation: a Department of Medical Microbiology , University of Debrecen , Debrecen , Hungary ;
authors.forename: László
authors.initials: L
authors.lastname: Majoros
authors.affiliation: a Department of Medical Microbiology , University of Debrecen , Debrecen , Hungary ;
authors.forename: Krisztina
authors.initials: K
authors.lastname: Szarka
authors.affiliation: a Department of Medical Microbiology , University of Debrecen , Debrecen , Hungary ;
authors.forename: Gábor
authors.initials: G
authors.lastname: Kardos
mesh_terms: D000293:Adolescent; D000328:Adult; D001426:Bacterial Proteins; D002353:Carrier State / Q000453:epidemiology / Q000382:microbiology; D003430:Cross-Sectional Studies; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000201:enzymology*; D004756:Enterobacteriaceae Infections / Q000453:epidemiology / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D006814:Hungary / Q000453:epidemiology; D008134:Long-Term Care / Q000706:statistics & numerical data*; D008297:Male; D008875:Middle Aged; D055815:Young Adult; D001618:beta-Lactamases
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases
keywords: Escherichia coli; Extended-spectrum beta-lactamases; Klebsiella pneumoniae