title: Comparative proteomic analysis of extracellular secreted proteins expressed by two pathogenic Acanthamoeba castellanii clinical isolates and a non-pathogenic ATCC strain.
abstract: Acanthamoeba keratitis (AK) is a serious ocular disease caused by pathogenic Acanthamoeba gaining entry through wounds in the corneal injury; generally, patients at risk for contracting AK wear contact lenses, usually over a long period of time. Moreover, pathogenic Acanthamoeba causes serious consequences: it makes the cornea turbid and difficult to operate on, including procedures such as enucleation of the eyeball. At present, diagnosis of this disease is not straightforward, and treatment is very demanding. We have established the comparative transcriptome and extracellular secreted proteomic database according to the non-pathogenic strain ATCC 30010 and the pathogenic strains NCKU_B and NCKU_D. We identified 44 secreted proteins successfully, 10 consensus secreted proteins and 34 strain-specific secreted proteins. These proteins may provide targets for therapy and immuno-diagnosis of Acanthamoeba infections. This study shows a suitable approach to identify secreted proteins in Acanthamoeba and provides new perspectives for the study of molecules potentially involved in the AK.
authors.affiliation: Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Huang
authors.affiliation: Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Department of Parasitology, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Wei-Chen
authors.initials: WC
authors.lastname: Lin
authors.affiliation: Genomics and Proteomics Core Laboratory, Department of Medical Research, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan.
authors.forename: Sung-Chou
authors.initials: SC
authors.lastname: Li
authors.affiliation: Department of Ophthalmology, National Cheng Kung University Hospital, Tainan, Taiwan.
authors.forename: Min-Hsiu
authors.initials: MH
authors.lastname: Shih
authors.affiliation: Genomics and Proteomics Core Laboratory, Department of Medical Research, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan.
authors.forename: Wen-Ching
authors.initials: WC
authors.lastname: Chan
authors.affiliation: Department of Parasitology, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Jyh-Wei
authors.initials: JW
authors.lastname: Shin
authors.affiliation: Department of Ophthalmology, National Cheng Kung University Hospital, Tainan, Taiwan. Electronic address: huangfc@mail.ncku.edu.tw.
authors.forename: Fu-Chin
authors.initials: FC
authors.lastname: Huang
mesh_terms: D048248:Acanthamoeba castellanii / Q000145:classification / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D015153:Blotting, Western; D019295:Computational Biology; D018076:DNA, Complementary / Q000096:biosynthesis; D015180:Electrophoresis, Gel, Two-Dimensional; D063990:Gene Ontology; D005838:Genotype; D040901:Proteomics; D015800:Protozoan Proteins / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D016053:RNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D059467:Transcriptome
chemical_list: D018076:DNA, Complementary; D015800:Protozoan Proteins; D016053:RNA, Protozoan
keywords: Acanthamoeba; Pathogenic strain; Proteomic analysis; Secreted proteins