title: Complete genome sequence of 'Mycobacterium neoaurum' NRRL B-3805, an androstenedione (AD) producer for industrial biotransformation of sterols.
abstract: Microbial bioconversion of sterols into high value steroid precursors, such as 4-androstene-3,17-dione (AD), is an industrial challenge. Genes and enzymes involved in sterol degradation have been proposed, although the complete pathway is not yet known. The genome sequencing of the AD producer strain 'Mycobacterium neoaurum' NRRL B-3805 (formerly Mycobacterium sp. NRRL B-3805) will serve to elucidate the critical steps for industrial processes and will provide the basis for further genetic engineering. The genome comprises a circular chromosome (5 421 338bp), is devoid of plasmids and contains 4844 protein-coding genes.
authors.affiliation: INBIOTEC (Instituto de Biotecnología de León), Parque Científico de León, Avda. Real 1, 24006 León, Spain; Área de Microbiología, Departamento de Biología Molecular, Universidad de León, Campus de Vegazana, 24071 León, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Rodríguez-García
authors.affiliation: INBIOTEC (Instituto de Biotecnología de León), Parque Científico de León, Avda. Real 1, 24006 León, Spain.
authors.forename: Estela
authors.initials: E
authors.lastname: Fernández-Alegre
authors.affiliation: INBIOTEC (Instituto de Biotecnología de León), Parque Científico de León, Avda. Real 1, 24006 León, Spain.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Morales
authors.affiliation: INBIOTEC (Instituto de Biotecnología de León), Parque Científico de León, Avda. Real 1, 24006 León, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Sola-Landa
authors.affiliation: Department of Biology, University of York, Wentworth Way, YO10 5DD, United Kingdom.
authors.forename: Jess
authors.initials: J
authors.lastname: Lorraine
authors.affiliation: Department of Biology, University of York, Wentworth Way, YO10 5DD, United Kingdom.
authors.forename: Sandy
authors.initials: S
authors.lastname: Macdonald
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Prospekt Nauki 5, 142290 Pushchino, Moscow Region, Russia.
authors.forename: Dmitry
authors.initials: D
authors.lastname: Dovbnya
authors.affiliation: Department of Biology, University of York, Wentworth Way, YO10 5DD, United Kingdom.
authors.forename: Margaret C M
authors.initials: MC
authors.lastname: Smith
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Prospekt Nauki 5, 142290 Pushchino, Moscow Region, Russia.
authors.forename: Marina
authors.initials: M
authors.lastname: Donova
authors.affiliation: INBIOTEC (Instituto de Biotecnología de León), Parque Científico de León, Avda. Real 1, 24006 León, Spain. Electronic address: c.barreiro@unileon.es.
authors.forename: Carlos
authors.initials: C
authors.lastname: Barreiro
mesh_terms: D000735:Androstenedione / Q000378:metabolism; D001482:Base Composition; D059646:Genome Size; D016680:Genome, Bacterial; D009161:Mycobacterium / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011544:Pseudogenes; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D000735:Androstenedione
keywords: 4-Androstene-3,17-dione; AD; ADD; Actinobacteria; Genome sequence; Mycobacterium; Steroids