title: Serum Helicobacter pylori FliD antibody and the risk of gastric cancer.
abstract: FliD and CagA are important virulence factors of H. pylori. We aimed to evaluate the screening values of FliD and CagA for gastric cancer (GC). Serum samples were obtained from 232 cases and 266 controls in a case-control study. Unconditional multivariate logistic regression with odds ratios (ORs) and 95% confidence intervals (CIs) was used to analyze the relationships between FliD, CagA and GC. The sensitivities, specificities and receiver operating characteristic (ROC) curves were calculated. Finally, the combined screening values of FliD, FlaA, NapA and CagA were assessed based on discriminant analysis. In all subjects, the associations of FliD and CagA with GC were evident with ORs (95% CIs) of 7.6 (4.7-12.3) and 2.5 (1.6-3.8), respectively (*p<0.001). The areas under ROC curves (AUCs) for FliD and CagA were 0.800 and 0.653, respectively. The AUC for the combination of FliD, FlaA and NapA was 0.915, which represented an increase of 0.115 over that of FliD alone (*p<0.001). These findings indicate that the FliD antibody is associated with GC and could exhibit high validity as a biomarker in screening for GC patients. The combination of FliD, FlaA and NapA improved the screening validity.
authors.affiliation: Department of Epidemiology, College of Public Health, Harbin Medical University, Harbin, Heilongjiang Province, P. R. China.
authors.forename: Hailin
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Epidemiology, College of Public Health, Harbin Medical University, Harbin, Heilongjiang Province, P. R. China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Epidemiology, College of Public Health, Harbin Medical University, Harbin, Heilongjiang Province, P. R. China.
authors.forename: Xiaomeng
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Epidemiology, College of Public Health, Harbin Medical University, Harbin, Heilongjiang Province, P. R. China.
authors.forename: Yingzi
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Xiangfang Center for Disease Control and Prevention, Harbin, Heilongjiang Province, P. R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Fan
authors.affiliation: Department of Epidemiology, College of Public Health, Harbin Medical University, Harbin, Heilongjiang Province, P. R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Guo
authors.affiliation: Department of Epidemiology, College of Public Health, Harbin Medical University, Harbin, Heilongjiang Province, P. R. China.
authors.forename: Xiyun
authors.initials: X
authors.lastname: Ren
authors.affiliation: Department of Epidemiology, College of Public Health, Harbin Medical University, Harbin, Heilongjiang Province, P. R. China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Epidemiology, College of Public Health, Harbin Medical University, Harbin, Heilongjiang Province, P. R. China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Tian
authors.affiliation: Department of Epidemiology, College of Public Health, Harbin Medical University, Harbin, Heilongjiang Province, P. R. China.
authors.forename: Yashuang
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood*; D019540:Area Under Curve; D001426:Bacterial Proteins / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000032:analysis*; D016022:Case-Control Studies; D005260:Female; D005408:Flagellin / Q000276:immunology; D016481:Helicobacter Infections / Q000150:complications* / Q000276:immunology; D016480:Helicobacter pylori; D006801:Humans; D008297:Male; D008875:Middle Aged; D012372:ROC Curve; D012680:Sensitivity and Specificity; D013274:Stomach Neoplasms / Q000097:blood / Q000382:microbiology*
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D014408:Biomarkers, Tumor; D005408:Flagellin; C067138:flaA protein, bacteria; C076733:FlaD protein, Bacteria
keywords: H. pylori CagA protein; H. pylori FliD protein; case-control study; gastric cancer; serum antibody