title: Tatarinan O, a lignin-like compound from the roots of Acorus tatarinowii Schott inhibits osteoclast differentiation through suppressing the expression of c-Fos and NFATc1.
abstract: Osteoclasts (OC) are large multinucleated cells derived from monocyte/macrophage precursors. Suppressing osteoclastogenesis is considered as an effective therapeutic approach to erosive bone disease. The root of Acorus tatarinowii Schott, a well-known traditional Chinese medicine was used to treat rheumatosis and other inflammatory disease. However, the effects of tatarinan O (TO), one of the lignin-like compounds isolated from the roots of Acorus tatarinowii Schott during bone development are still unclear. In the present study, we explored the effect of TO on RANKL-induced osteoclastogenesis in vitro. TO was found to suppress osteoclast differentiation from RANKL-stimulated mouse bone marrow macrophages (BMMs) without significant cytotoxicity. TO also dose-dependently suppressed bone resorption activity of mature osteoclasts. Additionally, TO apparently inhibited the expression of osteoclastic marker genes, such as MMP-9, Cts K and TRAP. Furthermore, our results showed that TO decreased RANKL-induced expression of c-Fos and NFATc1 without influencing NF-κB activation and MAPK phosphorylation. Hence, for the first time we revealed that TO dose-dependently inhibited osteoclastogenesis from RANKL-stimulated mouse BMMs via decreasing the expression of NFATc1 and c-Fos.
authors.affiliation: Key laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, PR China; School of Life Sciences and Technology, Tongji University, Shanghai 200092, PR China.
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Xu
authors.affiliation: Central Laboratory, The Second Clinical Hospital, Jilin University, Changchun 130041, PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.affiliation: Department of Gynaecology and obstetrics, China-Japan Union Hospital, Jilin University, Changchun 130033, PR China.
authors.forename: Yingjian
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, PR China.
authors.forename: Lei-Chang
authors.initials: LC
authors.lastname: Pan
authors.affiliation: Jilin Provincial Center for Disease Control and Prevention, 130062, PR China.
authors.forename: Donglin
authors.initials: D
authors.lastname: Wu
authors.affiliation: Key laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, PR China.
authors.forename: Qisheng
authors.initials: Q
authors.lastname: Peng
authors.affiliation: Key laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, PR China.
authors.forename: Maolin
authors.initials: M
authors.lastname: Zhang
authors.affiliation: School of Life Sciences and Technology, Tongji University, Shanghai 200092, PR China. Electronic address: hbwang@tongji.edu.cn.
authors.forename: Hong-Bing
authors.initials: HB
authors.lastname: Wang
authors.affiliation: Key laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, PR China. Electronic address: wanchunsun@jlu.edu.cn.
authors.forename: Wan-Chun
authors.initials: WC
authors.lastname: Sun
mesh_terms: D029023:Acorus / Q000276:immunology; D000818:Animals; D001862:Bone Resorption / Q000188:drug therapy*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D015536:Down-Regulation / Q000187:drug effects; D006801:Humans; D017705:Lignans / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000502:physiology; D008297:Male; D008516:Medicine, Chinese Traditional; D051379:Mice; D008810:Mice, Inbred C57BL; D050778:NFATC Transcription Factors / Q000235:genetics / Q000378:metabolism*; D010010:Osteoclasts / Q000187:drug effects* / Q000502:physiology; D018517:Plant Roots; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism*; D053245:RANK Ligand / Q000502:physiology
chemical_list: D017705:Lignans; D050778:NFATC Transcription Factors; D016760:Proto-Oncogene Proteins c-fos; D053245:RANK Ligand
keywords: Bone resorption; NFATc1; Osteoclast; RANKL; Tatarinan O; c-Fos