title: Kinetic ESI-MS Studies of Potent Anti-HIV Aptamers Based on the G-Quadruplex Forming Sequence d(TGGGAG).
abstract: To investigate what properties make tetramolecular G-quadruplex ODNs good anti-HIV aptamers, we studied the stoichiometry and the self-assembly kinetics of the highly active 5'-end modified G-quadruplexes based on the d(TGGGAG) sequence. Our results demonstrate that the 5'-end conjugation does not necessarily increase the folding rate of the G-quadruplex; indeed, it ascribes anti-HIV activity. Unexpectedly, the G4-folding kinetics of the inactive G4 is similar to that of the 5'-end modified sequences. ESI-MS studies also revealed the formation of higher order G4 structures identified as octameric complexes along with tetramolecular G-quadruplexes.
authors.affiliation: Department of Chemical Sciences, University of Napoli Federico II , Via Cintia, I-80126 Napoli, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Romanucci
authors.affiliation: IECB, ARNA Laboratory, University of Bordeaux, 33600 Pessac, France; Inserm, U869, ARNA Laboratory, Institut National de la Santé et de la Recherche Médicale, 33000 Bordeaux, France.
authors.forename: Adrien
authors.initials: A
authors.lastname: Marchand
authors.affiliation: IECB, ARNA Laboratory, University of Bordeaux, 33600 Pessac, France; Inserm, U869, ARNA Laboratory, Institut National de la Santé et de la Recherche Médicale, 33000 Bordeaux, France.
authors.forename: Oscar
authors.initials: O
authors.lastname: Mendoza
authors.affiliation: Department of Chemical Sciences, University of Napoli Federico II , Via Cintia, I-80126 Napoli, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: D'Alonzo
authors.affiliation: Department of Chemical Sciences, University of Napoli Federico II , Via Cintia, I-80126 Napoli, Italy.
authors.forename: Armando
authors.initials: A
authors.lastname: Zarrelli
authors.affiliation: IECB, ARNA Laboratory, University of Bordeaux, 33600 Pessac, France; Inserm, U869, ARNA Laboratory, Institut National de la Santé et de la Recherche Médicale, 33000 Bordeaux, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Gabelica
authors.affiliation: Department of Chemical Sciences, University of Napoli Federico II , Via Cintia, I-80126 Napoli, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Di Fabio
keywords: Anti-HIV aptamers; kinetic studies; mass spectrometry; modified oligonucleotides; tetramolecular G-quadruplexes