title: Effects of prenatal diclofenac sodium exposure on newborn testis: a histomorphometric study.
abstract: Diclofenac sodium (DS) is used primarily to treat fever and to alleviate pain and inflammation. We investigated the effects of DS exposure during gestation on the testes of rat pups to investigate the safety of its use during the prenatal period. Pregnant rats were separated into control, saline, low dose, medium dose and high dose groups. DS was given between weeks 15 and 21 of gestation. Total numbers of spermatogonia and Sertoli cells were counted in the testes of 7-day-old male rats using the physical disector method. By the end of the study, the total number of Sertoli cells was decreased significantly in a dose dependent manner in the medium and high dose groups compared to controls. No significant differences were found in the total number of spermatogonia in the control, saline and low dose DS groups. Medium and high dose DS administration reduced the total number of spermatogonia compared to other groups. We suggest that prenatal administration of DS can cause deleterious effects on the testis development, especially in high doses.
authors.affiliation: a Department of Molecular and Technical Medicine , Faculty of Medical Life Sciences, Hochschule Furtwangen University , Villingen-Schwenningen , Germany.
authors.forename: H
authors.initials: H
authors.lastname: Arslan
authors.affiliation: b Department of Histology and Embryology , School of Veterinary Medicine, İstanbul University Istanbul , Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Aktaş
authors.affiliation: c Department of Histology and Embryology , Medical School, Adıyaman University , Adıyaman , Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Elibol
authors.affiliation: b Department of Histology and Embryology , School of Veterinary Medicine, İstanbul University Istanbul , Turkey.
authors.forename: O B B
authors.initials: OB
authors.lastname: Esener
authors.affiliation: d Department of Histology and Embryology , Medical School, Ondokuz Mayıs University , Samsun , Turkey.
authors.forename: A P
authors.initials: AP
authors.lastname: Türkmen
authors.affiliation: d Department of Histology and Embryology , Medical School, Ondokuz Mayıs University , Samsun , Turkey.
authors.forename: K K
authors.initials: KK
authors.lastname: Yurt
authors.affiliation: d Department of Histology and Embryology , Medical School, Ondokuz Mayıs University , Samsun , Turkey.
authors.forename: M E
authors.initials: ME
authors.lastname: Onger
authors.affiliation: d Department of Histology and Embryology , Medical School, Ondokuz Mayıs University , Samsun , Turkey.
authors.forename: B Z
authors.initials: BZ
authors.lastname: Altunkaynak
authors.affiliation: d Department of Histology and Embryology , Medical School, Ondokuz Mayıs University , Samsun , Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Kaplan
mesh_terms: D000818:Animals; D004008:Diclofenac / Q000633:toxicity*; D005260:Female; D008297:Male; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D012708:Sertoli Cells / Q000187:drug effects / Q000473:pathology; D013737:Testis / Q000187:drug effects* / Q000196:embryology / Q000473:pathology
chemical_list: D004008:Diclofenac
keywords: Sertoli cells; diclofenac sodium; physical disector; rat; spermatogonia; stereology; testis