title: Dynamics of the T cell response to Mycobacterium tuberculosis DosR and Rpf antigens in a Colombian population of household contacts of recently diagnosed pulmonary tuberculosis patients.
abstract: Immune response to DosR and Rpf antigens from Mycobacterium tuberculosis (Mtb) seems to be important for latency maintenance. Little is known about the dynamics of the immune response to these antigens in an endemic community. Thus, the IFNγ response and cytokine production in response to PPD, Esat6-Cfp10 (E6-C10), DosR and Rpf antigens in healthy HHC of tuberculosis (TB) patients over a 12 (T12) months period (short-term, stLTBI) was investigated. This response was compared with a group of LTBI, who have remained healthy for 5-7 years (long-term, ltLTBI). According to the IFNγ response, two groups of HHCs were identified in stLTBI in response to E6-C10. At T12, E6-C10(+) HHCs displayed a decrease in the IFNγ levels and a generalized decrease in cytokines production. The E6-C10(-) HHC showed an increase in the IFNγ response and cytokine levels. In stLTBI, the responses to E6-C10, DosR, and Rpf may be interpreted as a protective immune response controlling Mtb infection and may be leading to a state of latent infection. Comparing the response of stLTBI and ltLTBI, we observed significant changes in the proportions of CD45RO(+)CD27(+) T cells to specific DosR and Rpf, which may indicate a persistent immune response to Mtb antigens in ltLTBI.
authors.affiliation: Grupo de Inmunología Celular e Inmunogenética (GICIG), Colombia. Electronic address: larroyoga@gmail.com.
authors.forename: Leonar
authors.initials: L
authors.lastname: Arroyo
authors.affiliation: Grupo de Inmunología Celular e Inmunogenética (GICIG), Colombia; Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Antioquia, UdeA, Calle 70 No. 52-21, Medellín, Colombia. Electronic address: mrojaslop@hotmail.com.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Rojas
authors.affiliation: Grupo de Inmunología Celular e Inmunogenética (GICIG), Colombia; Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Antioquia, UdeA, Calle 70 No. 52-21, Medellín, Colombia. Electronic address: blanca.ortiz@udea.edu.co.
authors.forename: Blanca L
authors.initials: BL
authors.lastname: Ortíz
authors.affiliation: Department of Infectious Diseases, Leiden University Medical Centre, Albinusdreef 2, 2333 ZA Leiden, Netherlands. Electronic address: c.l.m.c.franken@lumc.nl.
authors.forename: Kees L M C
authors.initials: KL
authors.lastname: Franken
authors.affiliation: Grupo de Inmunología Celular e Inmunogenética (GICIG), Colombia; Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Antioquia, UdeA, Calle 70 No. 52-21, Medellín, Colombia. Electronic address: lfernando.garcia@udea.edu.co.
authors.forename: Luis F
authors.initials: LF
authors.lastname: García
authors.affiliation: Department of Infectious Diseases, Leiden University Medical Centre, Albinusdreef 2, 2333 ZA Leiden, Netherlands. Electronic address: t.h.m.ottenhoff@lumc.nl.
authors.forename: Tom H M
authors.initials: TH
authors.lastname: Ottenhoff
authors.affiliation: Grupo de Inmunología Celular e Inmunogenética (GICIG), Colombia; Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Antioquia, UdeA, Calle 70 No. 52-21, Medellín, Colombia. Electronic address: luis.barrera@udea.edu.co.
authors.forename: Luis F
authors.initials: LF
authors.lastname: Barrera
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001426:Bacterial Proteins / Q000276:immunology*; D002478:Cells, Cultured; D003105:Colombia; D016358:Contact Tracing; D016207:Cytokines / Q000276:immunology*; D004268:DNA-Binding Proteins; D005191:Family Characteristics; D005260:Female; D006801:Humans; D055985:Latent Tuberculosis / Q000175:diagnosis / Q000276:immunology* / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D011494:Protein Kinases / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology* / Q000382:microbiology; D013997:Time Factors; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000276:immunology* / Q000382:microbiology; D055815:Young Adult
chemical_list: D001426:Bacterial Proteins; D016207:Cytokines; D004268:DNA-Binding Proteins; C542863:DosR protein, Mycobacterium tuberculosis; C113704:resuscitation-promoting factor, bacteria; D011494:Protein Kinases
keywords: DosR; Latent tuberculosis infection; M. tuberculosis; RD-1; Rpf; T-cell response