title: Quantitative Evaluation of BAFF, HMGB1, TLR 4 AND TLR 7 Expression in Patients with Relapsing Remitting Multiple Sclerosis.
abstract: Multiple sclerosis is a chronic inflammatory disease of the central nervous system characterized by a complex immune response. Because of the complex nature of MS pathogenesis, a panel of biomarkers derived from different platforms will be required to reflect disease-related alterations. Monitoring and evaluation of molecules associated with the pathogenesis of the disease would provide useful information on disease progression and therapeutic assessment. In view of this, we evaluated the mRNA expression levels of B-cell activating factor (BAFF), high mobility group box 1 (HMGB-1), Toll like receptor (TLR) 4 and TLR7 in MS. These molecules are implicated in the pathogenesis of MS; however, they havereceived little attention. PBMCs were isolated from whole blood of 84 Relapsing Remitting Multiple Sclerosis patients and 70 healthy controls. Relative quantitative RT-PCR was applied to quantify the transcriptional levels of the immune markers. The mRNA expression levels of TLR7 were significantly elevated in RRMS patients than healthy controls. Whereas, TLR4 expression was found to be significantly lower in the patients than control group. We found no difference analyzing the mRNA levels of BAFF and HMGB1. Our data highlights the immune marker correlates in RRMS patients. However, further in-depth studies are warranted to check for their reliability of biomarkers in autoimmune diseases such as MS.
authors.affiliation: Department of Pathobiology, Immunology Division, School of Public Health, Tehran University of Medical Sciences- International Campus (TUMS-IC), Tehran, Iran AND Department of Immunology, Faculty of Medical Laboratory Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria.
authors.forename: Kabir Magajir
authors.initials: KM
authors.lastname: Hamid
authors.affiliation: Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Nejati
authors.affiliation: Department of Virology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Zabihollah
authors.initials: Z
authors.lastname: Shoja
authors.affiliation: Immunology Department, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Yaghoub
authors.initials: Y
authors.lastname: Mollaei-Kandelousd
authors.affiliation: MS Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Rozita
authors.initials: R
authors.lastname: Doosti
authors.affiliation: Department of Pathobiology, Immunology Division, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Mirshafiey
authors.affiliation: Iranian Center of Neurological Research, Department of Neurology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Tafakhori
authors.affiliation: MS Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Sahraian
authors.affiliation: Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Sayed Mahdi
authors.initials: SM
authors.lastname: Marashi
mesh_terms: D000328:Adult; D053264:B-Cell Activating Factor / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005786:Gene Expression Regulation; D024243:HMGB1 Protein / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051197:Toll-Like Receptor 4 / Q000235:genetics*; D051199:Toll-Like Receptor 7 / Q000235:genetics*; D055815:Young Adult
chemical_list: D053264:B-Cell Activating Factor; D024243:HMGB1 Protein; C585491:HMGB1 protein, human; D012333:RNA, Messenger; C495345:TLR4 protein, human; C495348:TLR7 protein, human; C505719:TNFSF13B protein, human; D051197:Toll-Like Receptor 4; D051199:Toll-Like Receptor 7
keywords: Biomarkers; Cytokines; HMGB proteins; Inflammation; Multiple sclerosis; RNA messenger