title: An efficient dual-loaded multifunctional nanocarrier for combined photothermal and photodynamic therapy based on copper sulfide and chlorin e6.
abstract: The therapeutic effectiveness of photodynamic therapy (PDT) was hampered by the poor water solubility and instability in physiological conditions of the photosensitizers. Here, we designed folate conjugated thermosensitive liposomes (TSL) as the nanocarrier to improve the solubility, stability and biocompatibility of photosensitizer Chlorin e6 (Ce6). Based on the photothermal effect, we combined copper sulfide (CuS) as the photothermal agent to realize heat-triggered Ce6 release as well as synergistic effect of photothermal and photodynamic therapy. In vitro MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay showed that Ce6-CuS-TSL had low dark toxicity, while performed excellent phototoxicity under the combined 660 and 808 nm laser irradiation compared to any single laser irradiation alone. Moreover, in vivo combination therapy study revealed that Ce6-CuS-TSL inhibited tumor growth to a great extent without evident side effect under the laser irradiation. All detailed evidence demonstrated a considerable potential of Ce6-CuS-TSL for synergistic cancer treatment.
authors.affiliation: Tianjin Key Laboratory of Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, PR China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Tan
authors.affiliation: Tianjin Key Laboratory of Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, PR China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Pang
authors.affiliation: Tianjin Key Laboratory of Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, PR China.
authors.forename: Mingzhu
authors.initials: M
authors.lastname: Lei
authors.affiliation: Tianjin Key Laboratory of Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, PR China.
authors.forename: Man
authors.initials: M
authors.lastname: Ma
authors.affiliation: Tianjin Key Laboratory of Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Guo
authors.affiliation: Tianjin Key Laboratory of Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, PR China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Wang
authors.affiliation: Tianjin Key Laboratory of Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, PR China.
authors.forename: Meng
authors.initials: M
authors.lastname: Yu
authors.affiliation: Tianjin Key Laboratory of Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, PR China.
authors.forename: Fengping
authors.initials: F
authors.lastname: Tan
authors.affiliation: Tianjin Key Laboratory of Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, PR China. Electronic address: linan19850115@163.com.
authors.forename: Nan
authors.initials: N
authors.lastname: Li
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002735:Chlorophyllides; D003300:Copper / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D004355:Drug Stability; D005260:Female; D005492:Folic Acid / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008027:Light; D008081:Liposomes; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use / Q000648:ultrastructure; D009369:Neoplasms / Q000188:drug therapy*; D010778:Photochemotherapy; D011166:Porphyrins / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D017382:Reactive Oxygen Species / Q000378:metabolism; D012995:Solubility
chemical_list: D002735:Chlorophyllides; D008081:Liposomes; D011166:Porphyrins; D017382:Reactive Oxygen Species; C062985:phytochlorin; D003300:Copper; D005492:Folic Acid; C017846:cupric sulfide
keywords: 1,2-Dialmitoyl-sn-glycero-3-phosphocholine (PubChem CID: 452110); 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (PubChem CID: 406952); Chlorin e6; Chlorin e6 (PubChem CID: 5479494); Cholesterol (PubChem CID: 5997); Copper chloride (PubChem CID: 24014); Copper sulfide; Photodynamic therapy; Photothermal therapy; Sodium sulfide hydrate (PubChem CID: 73972); Thermosensitive liposomes