title: Urinary markers of nucleic acid oxidation in Danish overweight/obese children and youths.
abstract: Urinary excretion of the RNA and DNA oxidation markers, 8-oxo-7,8-dihydroguanosine (8-oxoGuo) and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in newly diagnosed adult type 2 diabetics are reported to be long-term predictors of mortality independent of conventional risk factors. In the current study, we investigated the relationships between urinary markers of nucleic acid oxidation concentrations and the degree of obesity and glucose metabolism in overweight compared to lean children. Forty-two (24 girls) overweight and 35 lean (19 girls) children and adolescents were recruited from the Registry of the Danish Childhood Obesity Biobank. Anthropometric measurements were collected at baseline and glucose metabolism was assessed by an oral glucose tolerance test. A urine sample was obtained during the test. Linear regression did not demonstrate any associations between the urinary markers and the degree of obesity or glucose metabolism in lean and obese children. However, sub-analyses adjusted for age, sex, and the degree of obesity showed positive associations between the 2 h glucose and the urinary markers, 8-oxoGuo (p = 0.02, r(2)= 0.63) and 8-oxodG (p = 0.046, r(2)= 0.48), and between the insulinogenic index and 8-oxoGuo (p = 0.03, r(2 )=( )0.60) in the 12 obese children exhibiting impaired glucose tolerance. Excretion of the urinary markers of nucleic acid oxidation and the degree of obesity or the glucose metabolism were not associated in this study. Nevertheless, obese children with impaired glucose tolerance seem to exhibiting an increased oxidative stress level, but due to the small sample size in this study, further investigations are required to elucidate this correlation.
authors.affiliation: a Department of Pediatrics, The Children's Obesity Clinic , Copenhagen University Hospital Holbæk , Holbæk , Denmark ;|b Department of Pediatrics , Copenhagen University Hospital Herlev , Herlev , Denmark ;
authors.forename: Julie Tonsgaard
authors.initials: JT
authors.lastname: Kloppenborg
authors.affiliation: a Department of Pediatrics, The Children's Obesity Clinic , Copenhagen University Hospital Holbæk , Holbæk , Denmark ;|c The Novo Nordisk Foundation Center for Basic Metabolic Research , University of Copenhagen , Copenhagen , Denmark ;
authors.forename: Cilius Esmann
authors.initials: CE
authors.lastname: Fonvig
authors.affiliation: b Department of Pediatrics , Copenhagen University Hospital Herlev , Herlev , Denmark ;|d Faculty of Health and Medical Sciences , University of Copenhagen , Copenhagen , Denmark ;
authors.forename: Jesper
authors.initials: J
authors.lastname: Johannesen
authors.affiliation: e Department of Clinical Biochemistry , Copenhagen University Hospital Holbæk , Holbæk , Denmark ;
authors.forename: Poul Jannik
authors.initials: PJ
authors.lastname: Bjerrum
authors.affiliation: d Faculty of Health and Medical Sciences , University of Copenhagen , Copenhagen , Denmark ;|f Laboratory of Clinical Pharmacology , Rigshospitalet , Copenhagen , Denmark ;|g Department of Clinical Pharmacology , Bispebjerg Hospital , Copenhagen , Denmark.
authors.forename: Henrik Enghusen
authors.initials: HE
authors.lastname: Poulsen
authors.affiliation: a Department of Pediatrics, The Children's Obesity Clinic , Copenhagen University Hospital Holbæk , Holbæk , Denmark ;|c The Novo Nordisk Foundation Center for Basic Metabolic Research , University of Copenhagen , Copenhagen , Denmark ;|d Faculty of Health and Medical Sciences , University of Copenhagen , Copenhagen , Denmark ;
authors.forename: Jens-Christian
authors.initials: JC
authors.lastname: Holm
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000293:Adolescent; D015415:Biomarkers / Q000652:urine; D002648:Child; D003430:Cross-Sectional Studies; D003718:Denmark; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D003924:Diabetes Mellitus, Type 2 / Q000652:urine*; D005260:Female; D006151:Guanosine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D008297:Male; D009765:Obesity / Q000652:urine*; D018384:Oxidative Stress / Q000502:physiology; D010865:Pilot Projects
chemical_list: D015415:Biomarkers; D006151:Guanosine; C046215:8-hydroxyguanosine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine
keywords: 8-oxoGuo; 8-oxodG; Child; glucose metabolism; obesity