title: Strong NFκB Expression is Associated With High-grade Dysplasia in Barrett's Esophagus.
abstract: Nuclear factor kappa B (NFκB) is a transcription factor that regulates the activation of genes involved in proinflammatory response and growth. In this study, we utilized immunohistochemical stains for 2 of the NFκB molecules (RELA and NFκB-1) to evaluate the expression of NFκB in Barrett's esophagus (BE). Forty-three cases of BE [17 cases with no dysplasia, 16 cases with low-grade dysplasia (LGD), and 10 cases with high-grade dysplasia (HGD)], 10 normal esophageal biopsies, and 9 cases of esophageal adenocarcinoma were evaluated. Expression of NFκB-1 and RELA did not occur in normal esophageal squamous mucosa. BE without dysplasia showed weak expression of RELA and NFκB-1 in 35% and 65% of cases, respectively. BE with LGD showed weak expression of RELA and NFκB-1 in 50% and 75% of cases, respectively. Strong expression of RELA and NFκB-1 did not occur in BE without dysplasia or with LGD. BE with HGD showed strong expression of RELA and NFκB-1 in 80% and 90% of cases, respectively. All cases of adenocarcinoma showed strong expression of both RELA and NFκB-1. There was a progressive increase in staining intensity of RELA and NFκB-1 along the metaplasia-dysplasia-adenocarcinoma pathway. Strong expression of NFκB is associated with HGD and adenocarcinoma (P<0.0001). We showed that strong expression of NFκB-1 and RELA correlates highly with BE with HGD and adenocarcinoma.
authors.affiliation: *Department of Pathology, University of Mississippi Medical Center, Jackson, MS †Department of Pathology, University of Louisville, Louisville, KY.
authors.forename: Siraj M
authors.initials: SM
authors.lastname: El Jamal
authors.forename: Alae A
authors.initials: AA
authors.lastname: Yaseen
authors.forename: Houda
authors.initials: H
authors.lastname: Alatassi
authors.forename: Stephen
authors.initials: S
authors.lastname: Slone
authors.forename: Barbara J
authors.initials: BJ
authors.lastname: Bishop
authors.forename: Mostafa
authors.initials: M
authors.lastname: Fraig
mesh_terms: D001471:Barrett Esophagus / Q000175:diagnosis / Q000503:physiopathology*; D000071041:Esophageal Mucosa / Q000378:metabolism / Q000503:physiopathology; D005786:Gene Expression Regulation; D006801:Humans; D006965:Hyperplasia / Q000473:pathology; D007150:Immunohistochemistry; D016328:NF-kappa B / Q000235:genetics* / Q000378:metabolism; D052002:NF-kappa B p50 Subunit / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D052002:NF-kappa B p50 Subunit; C489548:NFKB1 protein, human; C498143:RELA protein, human; D051996:Transcription Factor RelA