title: Application of a Mass Spectrometric Approach to Detect the Presence of Fatty Acid Biosynthetic Phosphopeptides.
abstract: The details of plant lipid metabolism are relatively well known but the regulation of fatty acid production at the protein level is still not understood. Hence this study explores the importance of phosphorylation as a mechanism to control the activity of fatty acid biosynthetic enzymes using low and high oleic acid mesocarps of oil palm fruit (Elaeis guineensis variety of Tenera). Adaptation of neutral loss-triggered tandem mass spectrometry and selected reaction monitoring to detect the neutral loss of phosphoric acid successfully found several phosphoamino acid-containing peptides. These peptides corresponded to the peptides from acetyl-CoA carboxylase and 3-enoyl-acyl carrier protein reductase as identified by their precursor ion masses. These findings suggest that these enzymes were phosphorylated at 20th week after anthesis. Phosphorylation could have reduce their activities towards the end of fatty acid biosynthesis at ripening stage. Implication of phosphorylation in the regulation of fatty acid biosynthesis at protein level has never been reported.
authors.affiliation: Malaysian Palm Oil Board, Persiaran Institusi, Bandar Baru Bangi, 43000, Kajang, Selangor, Malaysia. benjamin@mpob.gov.my.|AgResearch Lincoln Research Centre, Private Bag 4749, Christchurch, 8140, New Zealand. benjamin@mpob.gov.my.|Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln, New Zealand. benjamin@mpob.gov.my.
authors.forename: Benjamin Yii Chung
authors.identifier: http://orcid.org/0000-0001-7211-8284
authors.initials: BY
authors.lastname: Lau
authors.affiliation: AgResearch Lincoln Research Centre, Private Bag 4749, Christchurch, 8140, New Zealand.
authors.forename: Stefan
authors.initials: S
authors.lastname: Clerens
authors.affiliation: Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln, New Zealand.
authors.forename: James D
authors.initials: JD
authors.lastname: Morton
authors.affiliation: AgResearch Lincoln Research Centre, Private Bag 4749, Christchurch, 8140, New Zealand.|Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln, New Zealand.
authors.forename: Jolon M
authors.initials: JM
authors.lastname: Dyer
authors.affiliation: AgResearch Lincoln Research Centre, Private Bag 4749, Christchurch, 8140, New Zealand.
authors.forename: Santanu
authors.initials: S
authors.lastname: Deb-Choudhury
authors.affiliation: Malaysian Palm Oil Board, Persiaran Institusi, Bandar Baru Bangi, 43000, Kajang, Selangor, Malaysia.
authors.forename: Umi Salamah
authors.initials: US
authors.lastname: Ramli
mesh_terms: D028023:Arecaceae / Q000737:chemistry / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis*; D009829:Oleic Acids / Q000096:biosynthesis; D010748:Phosphopeptides / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D010766:Phosphorylation; D010940:Plant Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D012639:Seeds / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005227:Fatty Acids; D009829:Oleic Acids; D010748:Phosphopeptides; D010940:Plant Proteins
keywords: Fatty acid biosynthesis; Neutral loss; Neutral loss-triggered MS3; Oil palm; Phosphorylation; Selected reaction monitoring