title: Germline Competent Pluripotent Mouse Stem Cells Generated by Plasmid Vectors.
abstract: We developed nonintegrated methods to reprogram mouse embryonic fibroblast (MEF) cells into induced pluripotent stem cells (iPSCs) using pig pOct4, pSox2, and pc-Myc as well as human hKLF4, hAID, and hTDG that were carried by plasmid vectors. The 4F method employed pOct4, pSox2, pc-Myc, and hKLF4 to derive iPSC clones with naive embryonic stem cell (ESC)-like morphology. These 4F clones expressed endogenous mouse Nanog protein and could generate chimeras. In addition to the four conventional reprogramming factors used in the 4F method, hAID and hTDG were utilized in a 6F method to increase the conversion efficiency of reprogramming by approximately five-fold. One of the 6F plasmid derived iPSC (piPSC) clones was shown to be germline transmission competent.
authors.affiliation: a Division of Animal Technology, Laboratories of Animal Technology , Agricultural Technology Research Institute , Hsinchu City , Taiwan.
authors.forename: Chien-Hong
authors.initials: CH
authors.lastname: Chen
authors.affiliation: a Division of Animal Technology, Laboratories of Animal Technology , Agricultural Technology Research Institute , Hsinchu City , Taiwan.
authors.forename: Yu-Hsiu
authors.initials: YH
authors.lastname: Su
authors.affiliation: b Division of Biotechnology , Animal Technology Institute Taiwan , Chunan , Miaoli , Taiwan.
authors.forename: Kun-Hsiung
authors.initials: KH
authors.lastname: Lee
authors.affiliation: a Division of Animal Technology, Laboratories of Animal Technology , Agricultural Technology Research Institute , Hsinchu City , Taiwan.
authors.forename: Chin-kai
authors.initials: CK
authors.lastname: Chuang
mesh_terms: D000818:Animals; D000067470:Cellular Reprogramming Techniques / Q000379:methods*; D003001:Cloning, Molecular / Q000379:methods*; D018076:DNA, Complementary / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D057026:Induced Pluripotent Stem Cells; D051379:Mice; D010957:Plasmids / Q000235:genetics*; D013552:Swine
chemical_list: D018076:DNA, Complementary
keywords: Germline transmission; iPSC; plasmid