title: Ethanol/O2 biofuel cell using a biocathode consisting of laccase/ HOOC-MWCNTs/polydiallyldimethylammonium chloride.
abstract: In the present report we focused on the substitution of metallic catalysts by biocatalysts to develop a high efficient biofuel cell. A bioanode and a biocathode were designed using ADH and laccase, respectively. Carboxylated multiwall carbon nanotubes (HOOC-MWCNTs) and polydiallyldimethylammonium chloride (PDDA) were used for immobilizing the enzymes on either polymethylene green (PMG) modified glassy carbon or graphite electrodes. In this way, an ethanol-oxygen biofuel cell was designed in which PDDA/ADH/PDDA/HOOC-MWCNTs/PMG/GC and PDDA/Lac/PDDA/HOOC-MWCNTs/PMG/Gr operated as bioanode and biocathode, respectively. In the optimized condition of O2 saturated PBS (0.1M, pH 7.5) containing 1mM ethanol and 1mM NAD(+) the open-circuit voltage reached to a plateau at 504mV based of which the power density of 3.98mWcm(-2) was obtained.
authors.affiliation: Laboratory of Microanalysis, Institute of Biochemistry & Biophysics, University of Tehran, Tehran, Iran.
authors.forename: Farideh
authors.initials: F
authors.lastname: Gouranlou
authors.affiliation: Laboratory of Microanalysis, Institute of Biochemistry & Biophysics, University of Tehran, Tehran, Iran. Electronic address: ghourchian@ut.ac.ir.
authors.forename: Hedayatollah
authors.initials: H
authors.lastname: Ghourchian
mesh_terms: D000426:Alcohol Dehydrogenase; D001674:Bioelectric Energy Sources; D055664:Electrochemical Techniques; D004800:Enzymes, Immobilized; D000431:Ethanol; D042845:Laccase; D037742:Nanotubes, Carbon; D010100:Oxygen; D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds
chemical_list: D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; D000431:Ethanol; D000426:Alcohol Dehydrogenase; D042845:Laccase; D010100:Oxygen
keywords: Alcoholdehydrogenase; Biofuel cell; Carboxylated multiwall carbon nanotube; Laccase; Polydiallyldimethylammonium chloride; Polymethylene green