title: Antibiotic resistance mechanisms of Myroides sp.
abstract: Bacteria of the genus Myroides (Myroides spp.) are rare opportunistic pathogens. Myroides sp. infections have been reported mainly in China. Myroides sp. is highly resistant to most available antibiotics, but the resistance mechanisms are not fully elucidated. Current strain identification methods based on biochemical traits are unable to identify strains accurately at the species level. While 16S ribosomal RNA (rRNA) gene sequencing can accurately achieve this, it fails to give information on the status and mechanisms of antibiotic resistance, because the 16S rRNA sequence contains no information on resistance genes, resistance islands or enzymes. We hypothesized that obtaining the whole genome sequence of Myroides sp., using next generation sequencing methods, would help to clarify the mechanisms of pathogenesis and antibiotic resistance, and guide antibiotic selection to treat Myroides sp. infections. As Myroides sp. can survive in hospitals and the environment, there is a risk of nosocomial infections and pandemics. For better management of Myroides sp. infections, it is imperative to apply next generation sequencing technologies to clarify the antibiotic resistance mechanisms in these bacteria.
authors.affiliation: Yun Leung Laboratory for Molecular Diagnostics, School of Biomedical Sciences and Institute of Molecular Medicine, Huaqiao University / Engineering Research Center of Molecular Medicine, Ministry of Education, Xiamen 361021, China.
authors.forename: Shao-hua
authors.initials: SH
authors.lastname: Hu
authors.affiliation: Department of Neurosurgery, Linyi People's Hospital, Linyi 276000, China.
authors.forename: Shu-xing
authors.initials: SX
authors.lastname: Yuan
authors.affiliation: Linyi Health School of Shandong Province, Linyi 276000, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Qu
authors.affiliation: Yun Leung Laboratory for Molecular Diagnostics, School of Biomedical Sciences and Institute of Molecular Medicine, Huaqiao University / Engineering Research Center of Molecular Medicine, Ministry of Education, Xiamen 361021, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Yun Leung Laboratory for Molecular Diagnostics, School of Biomedical Sciences and Institute of Molecular Medicine, Huaqiao University / Engineering Research Center of Molecular Medicine, Ministry of Education, Xiamen 361021, China.
authors.forename: Ya-jun
authors.initials: YJ
authors.lastname: Zhou
authors.affiliation: Yun Leung Laboratory for Molecular Diagnostics, School of Biomedical Sciences and Institute of Molecular Medicine, Huaqiao University / Engineering Research Center of Molecular Medicine, Ministry of Education, Xiamen 361021, China.|Institute of Nanomedicine, Department of Medical Laboratory, Weifang Medical College, Weifang 261053, China.
authors.forename: Ming-xi
authors.initials: MX
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, Quanzhou First Hospital Affiliated to Fujian Medical University, Quanzhou 362000, China.
authors.forename: De-song
authors.initials: DS
authors.lastname: Ming
mesh_terms: D004352:Drug Resistance, Microbial; D041965:Flavobacteriaceae / Q000187:drug effects* / Q000235:genetics; D045826:Flavobacteriaceae Infections / Q000188:drug therapy; D016680:Genome, Bacterial; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008826:Microbial Sensitivity Tests
keywords: 16S ribosomal RNA gene sequencing; Antibiotic resistance; Identification methods; Myroides sp.; Next generation sequencing