title: Involvement of a universal amino acid synthesis impediment in cytoplasmic male sterility in pepper.
abstract: To explore the mechanisms of pepper (Capsicum annuum L.) cytoplasmic male sterility (CMS), we studied the different maturation processes of sterile and fertile pepper anthers. A paraffin section analysis of the sterile anthers indicated an abnormality of the tapetal layer and an over-vacuolization of the cells. The quantitative proteomics results showed that the expression of histidinol dehydrogenase (HDH), dihydroxy-acid dehydratase (DAD), aspartate aminotransferase (ATAAT), cysteine synthase (CS), delta-1-pyrroline-5-carboxylate synthase (P5CS), and glutamate synthetase (GS) in the amino acid synthesis pathway decreased by more than 1.5-fold. Furthermore, the mRNA and protein expression levels of DAD, ATAAT, CS and P5CS showed a 2- to 16-fold increase in the maintainer line anthers. We also found that most of the amino acid content levels decreased to varying degrees during the anther tapetum period of the sterile line, whereas these levels increased in the maintainer line. The results of our study indicate that during pepper anther development, changes in amino acid synthesis are significant and accompany abnormal tapetum maturity, which is most likely an important cause of male sterility in pepper.
authors.affiliation: Institute of Biology, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China.
authors.forename: Xianping
authors.initials: X
authors.lastname: Fang
authors.affiliation: Institute of Vegetables, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China.
authors.forename: Hong-Fei
authors.initials: HF
authors.lastname: Fu
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling 712100, China.
authors.forename: Zhen-Hui
authors.initials: ZH
authors.lastname: Gong
authors.affiliation: Institute of Vegetables, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China.
authors.forename: Wei-Guo
authors.initials: WG
authors.lastname: Chai
mesh_terms: D000596:Amino Acids / Q000096:biosynthesis*; D002212:Capsicum / Q000502:physiology*; D035264:Flowers / Q000254:growth & development / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D051479:Plant Infertility; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods
chemical_list: D000596:Amino Acids; D010940:Plant Proteins