title: Rapid and prodium iodide-compatible optical clearing method for brain tissue based on sugar/sugar-alcohol.
abstract: The developed optical clearing methods show great potential for imaging of large-volume tissues, but these methods present some nonnegligible limitations such as complexity of implementation and long incubation times. In this study, we tried to screen out rapid optical clearing agents by means of molecular dynamical simulation and experimental demonstration. According to the optical clearing potential of sugar and sugar-alcohol,we further evaluated the improvement in the optical clearing efficacy of mouse brain samples, imaging depth, fluorescence preservation, and linear deformation. The results showed that drops of sorbitol, sucrose, and fructose could quickly make the mouse brain sample transparent within 1 to 2 min, and induce about threefold enhancement in imaging depth. The former two could evidently enhance the fluorescence intensity of green fluorescent protein (GFP) and prodium iodide (PI) nuclear dye. Fructose could significantly increase the fluorescence intensity of PI, but slightly decrease the fluorescence intensity of GFP. Even though the three agents caused some shrinkage in samples, the contraction in horizontal and longitudinal directions are almost the same.
authors.affiliation: Huazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, 1037 Luoyu Road, Wuhan, Hubei 430074, ChinabHuazhong University of Science and Technology, MoE Key Laboratory for.
authors.forename: Tingting
authors.initials: T
authors.lastname: Yu
authors.affiliation: Huazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, 1037 Luoyu Road, Wuhan, Hubei 430074, ChinabHuazhong University of Science and Technology, MoE Key Laboratory for.
authors.forename: Yisong
authors.initials: Y
authors.lastname: Qi
authors.affiliation: Huazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, 1037 Luoyu Road, Wuhan, Hubei 430074, ChinabHuazhong University of Science and Technology, MoE Key Laboratory for.
authors.forename: Jianru
authors.initials: J
authors.lastname: Wang
authors.affiliation: Huazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, 1037 Luoyu Road, Wuhan, Hubei 430074, ChinabHuazhong University of Science and Technology, MoE Key Laboratory for.
authors.forename: Wei
authors.initials: W
authors.lastname: Feng
authors.affiliation: Huazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, 1037 Luoyu Road, Wuhan, Hubei 430074, ChinabHuazhong University of Science and Technology, MoE Key Laboratory for.
authors.forename: Jianyi
authors.initials: J
authors.lastname: Xu
authors.affiliation: Huazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, 1037 Luoyu Road, Wuhan, Hubei 430074, ChinabHuazhong University of Science and Technology, MoE Key Laboratory for.
authors.forename: Jingtan
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Huazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, 1037 Luoyu Road, Wuhan, Hubei 430074, ChinabHuazhong University of Science and Technology, MoE Key Laboratory for.
authors.forename: Yingtao
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Huazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, 1037 Luoyu Road, Wuhan, Hubei 430074, ChinabHuazhong University of Science and Technology, MoE Key Laboratory for.
authors.forename: Hui
authors.initials: H
authors.lastname: Gong
authors.affiliation: Huazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, 1037 Luoyu Road, Wuhan, Hubei 430074, ChinabHuazhong University of Science and Technology, MoE Key Laboratory for.
authors.forename: Qingming
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Huazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, 1037 Luoyu Road, Wuhan, Hubei 430074, ChinabHuazhong University of Science and Technology, MoE Key Laboratory for.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging*; D001923:Brain Chemistry / Q000502:physiology*; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000737:chemistry; D006651:Histocytochemistry / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D008853:Microscopy; D011419:Propidium / Q000737:chemistry*; D013402:Sugar Alcohols / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D013402:Sugar Alcohols; D049452:Green Fluorescent Proteins; D011419:Propidium