title: Influence of Tanshinone IIA on the Apoptosis of Human Esophageal Ec-109 Cells.
abstract: The induced-apoptosis effect and mechanism of human esophageal cancer Ec-109 cells via tanshinone IIA was investigated. The Ec-109 cells were cultured in vitro with different concentrations of tanshinone IIA (2 µg/mL, 4 µg/mL, or 8 µg/mL) for 12, 24, 36, and 48 hours. MTT assay was used to evaluate the proliferative inhibition rate of tanshinone IIA on esophageal Ec-109 cells. After 24 hours of culturing in vitro, a control group was assigned. The apoptosis rate was detected by the AO/EB and annexin V-FITC/propidium iodide assay, and the protein levels of Caspase-4 and CHOP were determined by the Western blot technique. MTT data showed that tanshinone IIA could significantly inhibit the proliferation of Ec-109 cells with a dose- and time-dependent mode. Compared with the control group, tanshinone IIA could apparently induce apoptosis of Ec-109 cells, and the level of Caspase-4 and CHOP (p < 0.01) obviously increased. Tanshinone IIA can significantly induce the apoptosis of Ec-109 cells, which may take effect by the stress pathway of the endoplasmic reticulum.
authors.forename: Yan-Qin
authors.initials: YQ
authors.lastname: Zhu
authors.forename: Bai-Yan
authors.initials: BY
authors.lastname: Wang
authors.forename: Fang
authors.initials: F
authors.lastname: Wu
authors.forename: Yong-Kang
authors.initials: YK
authors.lastname: An
authors.forename: Xin-Qiang
authors.initials: XQ
authors.lastname: Zhou
mesh_terms: D045784:Abietanes / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053471:Caspases, Initiator / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004938:Esophageal Neoplasms / Q000188:drug therapy*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D027544:Salvia / Q000737:chemistry; D051742:Transcription Factor CHOP / Q000235:genetics / Q000378:metabolism
chemical_list: D045784:Abietanes; C497249:DDIT3 protein, human; C021751:tanshinone; D051742:Transcription Factor CHOP; C093516:CASP4 protein, human; D053471:Caspases, Initiator