title: Role of heme Oxygenase-1 in low dose Radioadaptive response.
abstract: Radioadaptive response (RAR) is an important phenomenon induced by low dose radiation. However, the molecular mechanism of RAR is obscure. In this study, we focused on the possible role of heme oxygenase 1 (HO-1) in RAR. Consistent with previous studies, priming dose of X-ray radiation (1-10cGy) induced significant RAR in normal human skin fibroblasts (AG 1522 cells). Transcription and translation of HO-1 was up-regulated more than two fold by a priming dose of radiation (5cGy). Zinc protoporphyrin Ⅸ, a specific competitive inhibitor of HO-1, efficiently inhibited RAR whereas hemin, an inducer of HO-1, could mimic priming dose of X-rays to induce RAR. Knocking down of HO-1 by transfection of HO-1 siRNA significantly attenuated RAR. Furthermore, the expression of HO-1 gene was modulated by the nuclear factor (erythroid-derived 2)-like 2 (Nrf2), which translocated from cytoplasm to nucleus after priming dose radiation and enhance the antioxidant level of cells.
authors.affiliation: Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, China.
authors.forename: Lingzhi
authors.initials: L
authors.lastname: Bao
authors.affiliation: Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, China; University of Science and Technology of China, Hefei, Anhui, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
authors.affiliation: Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Chen
authors.affiliation: Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, China.
authors.forename: Jue
authors.initials: J
authors.lastname: Hou
authors.affiliation: Center for Radiological Research, Columbia University, New York, NY, USA.
authors.forename: Tom K
authors.initials: TK
authors.lastname: Hei
authors.affiliation: Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon Tong, Hong Kong; Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, China.
authors.forename: K N
authors.initials: KN
authors.lastname: Yu
authors.affiliation: Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, China; Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions and School for Radiological and Interdisciplinary Sciences (RAD-X), Soochow University, Suzhou, Jiangsu, China. Electronic address: hanw@hfcas.ac.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Han
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D002460:Cell Line; D002466:Cell Nucleolus / Q000378:metabolism / Q000528:radiation effects; D003593:Cytoplasm / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000378:metabolism* / Q000528:radiation effects; D005786:Gene Expression Regulation / Q000528:radiation effects; D051547:Heme Oxygenase-1 / Q000037:antagonists & inhibitors / Q000235:genetics*; D006427:Hemin / Q000235:genetics / Q000378:metabolism; D006801:Humans; D051267:NF-E2-Related Factor 2 / Q000235:genetics* / Q000378:metabolism; D011524:Protoporphyrins / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D014965:X-Rays
chemical_list: D000975:Antioxidants; D051267:NF-E2-Related Factor 2; C495635:NFE2L2 protein, human; D011524:Protoporphyrins; D034741:RNA, Small Interfering; C017803:zinc protoporphyrin; D006427:Hemin; D051547:Heme Oxygenase-1
keywords: Heme Oxygenase 1; Hemin; Nrf2; Radioadaptive response; Reactive oxidative species; Znpp