title: Promotion of tumour proliferation, migration and invasion by miR-92b in targeting RECK in osteosarcoma.
abstract: MicroRNAs play important roles in the development of cancers. Although miR-92b has been reported to promote the tumorigenesis of some cancers, its role in osteosarcoma remains unknown. In the present study, we focused on the expression, function and mechanisms of miR-92b in osteosarcoma development. The miRNA miR-92b was up-regulated in osteosarcoma cell lines and tissues; miR-92b up-regulation correlated with poor prognosis in osteosarcoma. Overexpression of miR-92b promoted osteosarcoma cell proliferation, migration and invasion, which was abrogated by miR-92b inhibition. Reversion-inducing, cysteine-rich protein with kazal motifs (RECK) was identified as the direct and functional target of miR-92b in osteosarcoma. Importantly, restoring RECK expression abrogated increases in cell growth, motility and invasiveness induced by miR-92b RECK was down-regulated in osteosarcoma tissues, and its expression level negatively correlated with miR-92b Collectively, our results indicate that miR-92b acts as an oncogenic miRNA and may be a therapeutic target in osteosarcoma.
authors.affiliation: Department of Orthopaedic Oncology, Changzheng Hospital, Second Military Medical University, Shanghai, China Department of Orthopaedics, 169th Hospital of PLA, Hengyang, Hunan, China Department of Orthopaedics, Xiangnan Hospital, College of Medicine, Hunan Normal University, Hengyang, Hunan, China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Orthopaedic Oncology, Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Wei
authors.affiliation: Department of Orthopaedics, Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu, China.
authors.forename: Sujia
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Orthopaedic Oncology, Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Orthopaedic Oncology, Changzheng Hospital, Second Military Medical University, Shanghai, China jianruxiao63@126.com.
authors.forename: Jianru
authors.initials: J
authors.lastname: Xiao
mesh_terms: D001859:Bone Neoplasms / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D058851:GPI-Linked Proteins / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D012516:Osteosarcoma / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D058851:GPI-Linked Proteins; C541874:MIRN92 microRNA, human; D035683:MicroRNAs; C115460:RECK protein, human
keywords: invasion; miR-92b; migration; osteosarcoma; prognosis; proliferation