title: Stromal Integrin α11β1 Affects RM11 Prostate and 4T1 Breast Xenograft Tumors Differently.
abstract: OBJECTIVE
  It has been implied that the collagen binding integrin α11β1 plays a role in carcinogenesis. As still relatively little is known about how the stromal integrin α11β1 affects different aspects of tumor development, we wanted to examine the direct effects on primary tumor growth, fibrosis, tumor interstitial fluid pressure (PIF) and metastasis in murine 4T1 mammary and RM11 prostate tumors, using an in vivo SCID integrin α11-deficient mouse model.
  
  
  METHODS
  Tumor growth was measured using a caliper, PIF by the wick-in-needle technique, activated fibroblasts by α-SMA immunofluorescence staining and fibrosis by transmission electron microscopy and picrosirius-red staining. Metastases were evaluated using hematoxylin and eosin stained sections.
  
  
  RESULTS
  RM11 tumor growth was significantly reduced in the SCID integrin α11-deficient (α11-KO) compared to in SCID integrin α11 wild type (WT) mice, whereas there was no similar effect in the 4T1 tumor model. The 4T1 model demonstrated an alteration in collagen fibril diameter in the integrin α11-KO mice compared to WT, which was not found in the RM11 model. There were no significant differences in the amount of activated fibroblasts, total collagen content, collagen organization or PIF in the tumors in integrin α11-deficient mice compared to WT mice. There was also no difference in lung metastases between the two groups.
  
  
  CONCLUSIONS
  Deficiency of stromal integrin α11β1 showed different effects on tumor growth and collagen fibril diameter depending on tumor type, but no effect on tumor PIF or development of lung metastasis.
authors.affiliation: Department of Biomedicine, University of Bergen, Bergen, Norway.|Matrix biology group, Haukeland University Hospital, Bergen, Norway.
authors.forename: Inga
authors.initials: I
authors.lastname: Reigstad
authors.affiliation: Department of Biomedicine, University of Bergen, Bergen, Norway.|Center of Cancer Biomarkers, University of Bergen, Bergen, Norway.
authors.forename: Hilde Y H
authors.initials: HY
authors.lastname: Smeland
authors.affiliation: Department of Biomedicine, University of Bergen, Bergen, Norway.|Matrix biology group, Haukeland University Hospital, Bergen, Norway.
authors.forename: Trude
authors.initials: T
authors.lastname: Skogstrand
authors.affiliation: Department of Biomedicine, University of Bergen, Bergen, Norway.|Matrix biology group, Haukeland University Hospital, Bergen, Norway.
authors.forename: Kristina
authors.initials: K
authors.lastname: Sortland
authors.affiliation: Department of Biomedicine, University of Bergen, Bergen, Norway.|Center of Cancer Biomarkers, University of Bergen, Bergen, Norway.
authors.forename: Marei Caroline
authors.initials: MC
authors.lastname: Schmid
authors.affiliation: Department of Biomedicine, University of Bergen, Bergen, Norway.|Center of Cancer Biomarkers, University of Bergen, Bergen, Norway.
authors.forename: Rolf K
authors.initials: RK
authors.lastname: Reed
authors.affiliation: Department of Biomedicine, University of Bergen, Bergen, Norway.
authors.forename: Linda
authors.initials: L
authors.lastname: Stuhr
mesh_terms: D000199:Actins / Q000096:biosynthesis; D000818:Animals; D063646:Carcinogenesis / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D003094:Collagen / Q000378:metabolism*; D005260:Female; D064593:Heterografts; D016023:Integrins / Q000096:biosynthesis / Q000235:genetics*; D008175:Lung Neoplasms / Q000556:secondary; D008297:Male; D015674:Mammary Neoplasms, Animal / Q000473:pathology*; D051379:Mice; D018345:Mice, Knockout; D016513:Mice, SCID; D046529:Microscopy, Electron, Transmission; D011471:Prostatic Neoplasms / Q000473:pathology*; D038981:Receptors, Collagen / Q000096:biosynthesis / Q000235:genetics*; D047368:Tumor Burden / Q000235:genetics
chemical_list: D000199:Actins; D016023:Integrins; D038981:Receptors, Collagen; C467800:integrin alpha11beta1; D003094:Collagen