title: Dissecting Electrostatic Contributions to Folding and Self-Assembly Using Designed Multicomponent Peptide Systems.
abstract: We investigate formation of nano- to microscale peptide fibers and sheets where assembly requires association of two distinct collagen mimetic peptides (CMPs). The multicomponent nature of these designs allows the decoupling of amino acid contributions to peptide folding versus higher-order assembly. While both arginine and lysine containing CMP sequences can favor triple-helix folding, only arginine promotes rapid supramolecular assembly in each of the three two-component systems examined. Unlike lysine, the polyvalent guanidyl group of arginine is capable of both intra- and intermolecular contacts, promoting assembly. This is consistent with the supramolecular diversity of CMP morphologies observed throughout the literature. It also connects CMP self-assembly with a broad range of biomolecular interaction phenomena, providing general principles for modeling and design.
authors.affiliation: Department of Physics, Indian Institute of Technology (Banaras Hindu University) , Varanasi 221005, Uttar Pradesh, India.
authors.forename: Avanish S
authors.initials: AS
authors.lastname: Parmar
authors.affiliation: Center for Advanced Biotechnology and Medicine, Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical School, Rutgers University , 679 Hoes Lane West, Piscataway, New Jersey 08854, United States.
authors.forename: Jose K
authors.initials: JK
authors.lastname: James
authors.affiliation: Center for Advanced Biotechnology and Medicine, Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical School, Rutgers University , 679 Hoes Lane West, Piscataway, New Jersey 08854, United States.
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Grisham
authors.affiliation: Center for Advanced Biotechnology and Medicine, Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical School, Rutgers University , 679 Hoes Lane West, Piscataway, New Jersey 08854, United States.
authors.forename: Douglas H
authors.initials: DH
authors.lastname: Pike
authors.affiliation: Center for Advanced Biotechnology and Medicine, Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical School, Rutgers University , 679 Hoes Lane West, Piscataway, New Jersey 08854, United States.
authors.forename: Vikas
authors.initials: V
authors.lastname: Nanda
mesh_terms: D000595:Amino Acid Sequence; D032701:Biomimetics; D003094:Collagen / Q000737:chemistry*; D008239:Lysine / Q000737:chemistry*; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry*; D055672:Static Electricity
chemical_list: D010455:Peptides; D003094:Collagen; D008239:Lysine