title: Intervolume analysis to achieve four-dimensional optical microangiography for observation of dynamic blood flow.
abstract: We demonstrate in vivo volumetric optical microangiography at ∼ 200 volumes/s by the use of 1.6 MHz Fourier domain mode-locking swept source optical coherence tomography and an effective 36 kHz microelectromechanical system (MEMS) scanner. We propose an intervolume analysis strategy to contrast the dynamic blood flow signal from the static tissue background. The proposed system is demonstrated by imaging cerebral blood flow in mice in vivo. For the first time, imaging speed, sensitivity, and temporal resolution become possible for a direct four-dimensional observation of microcirculations within live body parts.
authors.affiliation: University of Washington, Department of Bioengineering, 3720 15th Avenue NE, Seattle, Washington 98195, United States.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: University of Washington, Department of Bioengineering, 3720 15th Avenue NE, Seattle, Washington 98195, United States.
authors.forename: Jingjiang
authors.initials: J
authors.lastname: Xu
authors.affiliation: University of Washington, Department of Bioengineering, 3720 15th Avenue NE, Seattle, Washington 98195, United StatesbUniversity of Washington, Department of Electrical Engineering, 185 Stevens Way, Seattle, Washington 98195, United States.
authors.forename: Utku
authors.initials: U
authors.lastname: Baran
authors.affiliation: University of Washington, Department of Bioengineering, 3720 15th Avenue NE, Seattle, Washington 98195, United States.
authors.forename: Shaozhen
authors.initials: S
authors.lastname: Song
authors.affiliation: University of Washington, Department of Bioengineering, 3720 15th Avenue NE, Seattle, Washington 98195, United States.
authors.forename: Wan
authors.initials: W
authors.lastname: Qin
authors.affiliation: University of Washington, Department of Bioengineering, 3720 15th Avenue NE, Seattle, Washington 98195, United States.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Qi
authors.affiliation: University of Washington, Department of Bioengineering, 3720 15th Avenue NE, Seattle, Washington 98195, United States.
authors.forename: Ruikang K
authors.initials: RK
authors.lastname: Wang
mesh_terms: D000465:Algorithms; D000792:Angiography / Q000379:methods*; D000818:Animals; D002540:Cerebral Cortex / Q000098:blood supply / Q000000981:diagnostic imaging; D002560:Cerebrovascular Circulation / Q000502:physiology; D004867:Equipment Design; D021621:Imaging, Three-Dimensional / Q000379:methods*; D051379:Mice; D008833:Microcirculation / Q000502:physiology; D041623:Tomography, Optical Coherence / Q000379:methods*