title: Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets.
abstract: Here, we demonstrate a simple method for the rapid production of size-controllable, monodisperse, W/O microdroplets using a capillary-based centrifugal microfluidic device. W/O microdroplets have recently been used in powerful methods that enable miniaturized chemical experiments. Therefore, developing a versatile method to yield monodisperse W/O microdroplets is needed. We have developed a method for generating monodisperse W/O microdroplets based on a capillary-based centrifugal axisymmetric co-flowing microfluidic device. We succeeded in controlling the size of microdroplets by adjusting the capillary orifice. Our method requires equipment that is easier-to-use than with other microfluidic techniques, requires only a small volume (0.1-1 µl) of sample solution for encapsulation, and enables the production of hundreds of thousands number of W/O microdroplets per second. We expect this method will assist biological studies that require precious biological samples by conserving the volume of the samples for rapid quantitative analysis biochemical and biological studies.
authors.affiliation: Department of Computational Intelligence and Systems Science, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology.
authors.forename: Masamune
authors.initials: M
authors.lastname: Morita
authors.affiliation: Department of Computational Intelligence and Systems Science, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology; Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology.
authors.forename: Hitoyoshi
authors.initials: H
authors.lastname: Yamashita
authors.affiliation: Department of Computational Intelligence and Systems Science, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Hayakawa
authors.affiliation: Department of Mechanical Engineering, Keio University.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Onoe
authors.affiliation: Department of Computational Intelligence and Systems Science, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology; PRESTO, Japan Science and Technology Agency; takinoue@dis.titech.ac.jp.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Takinoue
mesh_terms: D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D044085:Microfluidics / Q000379:methods*; D012996:Solutions / Q000737:chemistry*
chemical_list: D012996:Solutions