title: Organ Length Control by an ADAMTS Extracellular Protease in Caenorhabditis elegans.
abstract: MIG-17, a secreted protease of the ADAMTS family, acts in the directed migration of gonadal distal tip cells (DTCs) through regulation of the gonadal basement membrane in Caenorhabditis elegans Here, we show that MIG-17 is also required for the control of pharynx elongation during animal growth. We found that the pharynx was elongated in mig-17 mutants compared with wild type. MIG-17 localized to the pharyngeal basement membrane as well as to the gonadal basement membrane. The number of nuclei in the pharynx, and the pumping rate of the pharynx, were not affected in mig-17 mutants, suggesting that cells constituting the pharynx are elongated, although the pharynx functions normally in these mutants. In contrast to the control of DTC migration, MIG-18, a secreted cofactor of MIG-17, was not essential for pharynx length regulation. In addition, the downstream pathways of MIG-17 involving LET-2/type IV collagen, FBL-1/fibulin-1, and NID-1/nidogen, partly diverged from those in gonad development. These results indicate that basement membrane remodeling is important for organ length regulation, and suggest that MIG-17/ADAMTS functions in similar but distinct molecular machineries in pharyngeal and gonadal basement membranes.
authors.affiliation: Department of Bioscience, Kwansei Gakuin University, 2-1 Gakuen, Sanda 669-1337, Japan.
authors.forename: Yukimasa
authors.initials: Y
authors.lastname: Shibata
authors.affiliation: Department of Bioscience, Kwansei Gakuin University, 2-1 Gakuen, Sanda 669-1337, Japan.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Kawakado
authors.affiliation: Department of Bioscience, Kwansei Gakuin University, 2-1 Gakuen, Sanda 669-1337, Japan.
authors.forename: Noriyoshi
authors.initials: N
authors.lastname: Hori
authors.affiliation: Department of Bioscience, Kwansei Gakuin University, 2-1 Gakuen, Sanda 669-1337, Japan.
authors.forename: Kota
authors.initials: K
authors.lastname: Tanaka
authors.affiliation: Department of Bioscience, Kwansei Gakuin University, 2-1 Gakuen, Sanda 669-1337, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Inoue
authors.affiliation: Department of Bioscience, Kwansei Gakuin University, 2-1 Gakuen, Sanda 669-1337, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Takano
authors.affiliation: Department of Bioscience, Kwansei Gakuin University, 2-1 Gakuen, Sanda 669-1337, Japan Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan.
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Kubota
authors.affiliation: Department of Bioscience, Kwansei Gakuin University, 2-1 Gakuen, Sanda 669-1337, Japan nishiwaki@kwansei.ac.jp.
authors.forename: Kiyoji
authors.initials: K
authors.lastname: Nishiwaki
mesh_terms: D000071096:ADAMTS Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D001485:Basement Membrane / Q000378:metabolism; D017173:Caenorhabditis elegans / Q000033:anatomy & histology* / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D002384:Catalysis; D056726:Genetic Association Studies; D006031:Glycosylation; D006066:Gonads / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D054730:Protein Interaction Domains and Motifs; D021381:Protein Transport; D019655:Quantitative Trait, Heritable
chemical_list: D029742:Caenorhabditis elegans Proteins; D000071096:ADAMTS Proteins
keywords: ADAMTS protease; basement membrane; organ elongation; pharynx