title: NMR assignments of the macro domain from Middle East respiratory syndrome coronavirus (MERS-CoV).
abstract: The newly emerging human pathogen, Middle East respiratory syndrome coronavirus (MERS-CoV), contains a macro domain in the highly conserved N-terminal region of non-structural protein 3. Intense research has shown that macro domains bind ADP-ribose and other derivatives, but it still remains intangible about their exact function. In this study we report the preliminary structural analysis through solution NMR spectroscopy of the MERS-CoV macro domain. The near complete NMR assignments of MERS-CoV macro domain provide the basis for subsequent structural and biochemical investigation in the context of protein function.
authors.affiliation: Genomics Research Center, Academia Sinica, Nankang, Taipei, 11529, Taiwan.
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Huang
authors.affiliation: Genome and Systems Biology Degree Program, National Taiwan University and Academia Sinica, Taipei, 10617, Taiwan.|Department of Agricultural Chemistry, National Taiwan University, Taipei, 10617, Taiwan.
authors.forename: Chao-Cheng
authors.initials: CC
authors.lastname: Cho
authors.affiliation: Genomics Research Center, Academia Sinica, Nankang, Taipei, 11529, Taiwan. chifon@gate.sinica.edu.tw.
authors.forename: Chi-Fon
authors.initials: CF
authors.lastname: Chang
authors.affiliation: Genome and Systems Biology Degree Program, National Taiwan University and Academia Sinica, Taipei, 10617, Taiwan. andyhsu@ntu.edu.tw.|Department of Agricultural Chemistry, National Taiwan University, Taipei, 10617, Taiwan. andyhsu@ntu.edu.tw.|Center for Systems Biology, National Taiwan University, Taipei, 10617, Taiwan. andyhsu@ntu.edu.tw.
authors.forename: Chun-Hua
authors.identifier: 0000-0002-0008-7383
authors.initials: CH
authors.lastname: Hsu
mesh_terms: D065207:Middle East Respiratory Syndrome Coronavirus / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D000072417:Protein Domains; D017361:Viral Nonstructural Proteins / Q000737:chemistry*
chemical_list: D017361:Viral Nonstructural Proteins
keywords: ADP-ribose binding module; MERS-CoV; NMR spectroscopy; Viral macro domain