title: Studies on influence of polymer modifiers for fluorescent nanocrystals' cytotoxicity.
abstract: The presented studies aimed at investigation of the effect of CdSeS/ZnS quantum dots (QDs) stabilized with hyperbranched polyglycidol and its carboxylated derivative on adenocarcinomic human alveolar basal epithelial cells (A549). The first stage of studies concerned the modification of quantum dots with both types of the tested polymers with the use of pyridine as an intermediate agent. Subsequently, cytotoxic effect of the prepared nanoparticles was examined after various incubation time using MTT test (cell metabolic activity assay). Our studies revealed that CdSeS/ZnS with a diameter of 6nm, which were stabilized with hyperbranched polymers do not penetrate into cells, even after prolonged incubation time. Moreover, the cytotoxic effect of the tested QDs was observed over a range of tested concentrations (5-90μM of Cd(2+)). It was confirmed that tested nanoparticles had significant influence on cell culture viability. The examined cytotoxic effect of the tested quantum dots was dependent on the type of polymer applied and the experiments indicated, that the one bearing carboxylic moieties is more toxic to A549 cells.
authors.affiliation: Warsaw University of Technology, Faculty of Chemistry, Department of Microbioanalytics, Noakowskiego 3, 00 664 Warsaw, Poland. Electronic address: ilona@ch.pw.edu.pl.
authors.forename: Ilona
authors.initials: I
authors.lastname: Grabowska-Jadach
authors.affiliation: Warsaw University of Technology, Faculty of Chemistry, Department of Microbioanalytics, Noakowskiego 3, 00 664 Warsaw, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Drozd
authors.affiliation: Warsaw University of Technology, Faculty of Chemistry, Department of Microbioanalytics, Noakowskiego 3, 00 664 Warsaw, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Biegala
authors.affiliation: Warsaw University of Technology, Faculty of Chemistry, Department of Microbioanalytics, Noakowskiego 3, 00 664 Warsaw, Poland.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Pietrzak
authors.affiliation: Warsaw University of Technology, Faculty of Materials Science and Engineering, Woloska 141, 02-507 Warsaw, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Mazurkiewicz-Pawlicka
authors.affiliation: Warsaw University of Technology, Faculty of Chemistry, Chair of Polymer Chemistry and Technology, Noakowskiego 3, 00 664 Warsaw, Poland.
authors.forename: Paweł G
authors.initials: PG
authors.lastname: Parzuchowski
authors.affiliation: Warsaw University of Technology, Faculty of Chemistry, Department of Microbioanalytics, Noakowskiego 3, 00 664 Warsaw, Poland.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Brzózka
mesh_terms: D000072283:A549 Cells; D019187:Cadmium Compounds / Q000737:chemistry / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes / Q000737:chemistry / Q000633:toxicity*; D006801:Humans; D010316:Particle Size; D011409:Propylene Glycols / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry / Q000633:toxicity*; D018036:Selenium Compounds / Q000737:chemistry / Q000633:toxicity*; D013440:Sulfides / Q000737:chemistry / Q000633:toxicity*; D013499:Surface Properties; D017967:Zinc Compounds / Q000737:chemistry / Q000633:toxicity*
chemical_list: D019187:Cadmium Compounds; D005456:Fluorescent Dyes; D011409:Propylene Glycols; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; C502907:polyglycidol; C058667:cadmium selenide; C031238:zinc sulfide
keywords: Cytotoxicity; Hyperbranched polymer; Polymer coating; Quantum dots