title: [ITS sequences variation and phylogenetic analysis on 31 geographical populations of Notopterygium incisum].
abstract: In this study, 31 Notopterygium incisum populations were analyzed using ITS sequences to investigate the genetic structure. The results showed that: the ITS region ranged in size from 634 to 635 bp and base composition was with high G + C content of 57.8%. Thirty-one polymorphic sites were detected from 402 sequences of 31 populations of N. incisum, and the proportion of polymorphic sites was 4.88%, in which parsimony informative sites were up to 12. And 31 haplotypes were identified based on these polymorphic sites. Molecular variance analysis (AMOVA) indicated that high genetic differentiation (57%) existed among population, and gene flow was low (N(m) = 0.38) among populations. Phylogenetic relationships of 31 haplotypes were analyzed using NJ method with N. forbesiias an out-group. Phylogenetic analysis showed that 31 haplotypes from different populations mixed together and did not form distinct geographically separated clades.
authors.forename: Lu-cun
authors.initials: LC
authors.lastname: Yang
authors.forename: He-chun
authors.initials: HC
authors.lastname: Liu
authors.forename: Xue-li
authors.initials: XL
authors.lastname: Zhou
authors.forename: Wen-hua
authors.initials: WH
authors.lastname: Xu
authors.forename: Guo-ying
authors.initials: GY
authors.lastname: Zhou
mesh_terms: D019661:Apiaceae / Q000145:classification* / Q000235:genetics*; D001483:Base Sequence; D002681:China; D021901:DNA, Intergenic / Q000235:genetics*; D051456:Gene Flow; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny
chemical_list: D021901:DNA, Intergenic