title: The lack of aspirin resistance in patients with coronary artery disease.
abstract: BACKGROUND
  Aspirin resistance established by different laboratory methods is still a debated problem. Using COX1 specific methods no aspirin resistance was detected among healthy volunteers. Here we tested the effect of chronic aspirin treatment on platelets from patients with stable coronary artery disease. The expression of COX2 mRNA in platelets and its influences on the effect of aspirin was also investigated.
  
  
  METHODS
  One hundred and forty four patients were enrolled in the study. The direct measurement of COX1 acetylation was carried out by monoclonal antibodies specific to acetylated and non-acetylated COX1 (acCOX1 and nacCOX1) using Western blotting technique. Arachidonic acid (AA) induced TXB2 production by platelets was measured by competitive immunoassay. AA induced platelet aggregation, ATP secretion and VerifyNow Aspirin Assay were also performed. COX2 and COX1 mRNA expression in platelets were measured in 56 patients by RT-qPCR.
  
  
  RESULTS
  In 138 patients only acCOX1 was detected, in the remaining six patients nacCOX1 disappeared after a compliance period. AA induced TXB2 production by platelets was very low in all patients including the 6 patients after compliance. AA induced platelet aggregation, secretion and with a few exceptions the VerifyNow Assay also demonstrated the effect of aspirin. Smoking, diabetes mellitus and inflammatory conditions did not influence the results. The very low amount of COX2 mRNA detected in 39 % of the investigated platelets did not influence the effect of aspirin.
  
  
  CONCLUSIONS
  No aspirin resistance was detected among patients with stable coronary artery disease. COX2 expression in platelets did not influence the effect of aspirin.
authors.affiliation: Institute of Cardiology and Heart Surgery, University of Debrecen, 22 Móricz Zsigmond Krt., 4032, Debrecen, Hungary.
authors.forename: Nóra
authors.initials: N
authors.lastname: Homoródi
authors.affiliation: Institute of Cardiology and Heart Surgery, University of Debrecen, 22 Móricz Zsigmond Krt., 4032, Debrecen, Hungary.|Division of Clinical Laboratory Science, Department of Laboratory Medicine, University of Debrecen, 98 Nagyerdei Krt., 4032, Debrecen, Hungary.
authors.forename: Emese G
authors.initials: EG
authors.lastname: Kovács
authors.affiliation: Department of Cardiology, Military Hospital, 44 Róbert Károly Krt., 1134, Budapest, Hungary.
authors.forename: Sarolta
authors.initials: S
authors.lastname: Leé
authors.affiliation: Division of Clinical Laboratory Science, Department of Laboratory Medicine, University of Debrecen, 98 Nagyerdei Krt., 4032, Debrecen, Hungary.
authors.forename: Éva
authors.initials: É
authors.lastname: Katona
authors.affiliation: Vascular Biology, Thrombosis and Hemostasis Research Group of the Hungarian Academy of Science, University of Debrecen, 98 Nagyerdei Krt., 4032, Debrecen, Hungary.
authors.forename: Amir H
authors.initials: AH
authors.lastname: Shemirani
authors.affiliation: Division of Clinical Laboratory Science, Department of Laboratory Medicine, University of Debrecen, 98 Nagyerdei Krt., 4032, Debrecen, Hungary.
authors.forename: Gizella
authors.initials: G
authors.lastname: Haramura
authors.affiliation: Institute of Cardiology and Heart Surgery, University of Debrecen, 22 Móricz Zsigmond Krt., 4032, Debrecen, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Balogh
authors.affiliation: Division of Clinical Laboratory Science, Department of Laboratory Medicine, University of Debrecen, 98 Nagyerdei Krt., 4032, Debrecen, Hungary.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Bereczky
authors.affiliation: Diagnosticum Co., Research Laboratory, 126 Attila u., 1046, Budapest, Hungary.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Szőke
authors.affiliation: Diagnosticum Co., Research Laboratory, 126 Attila u., 1046, Budapest, Hungary.
authors.forename: Hajna
authors.initials: H
authors.lastname: Péterfy
authors.affiliation: Department of Cardiology, Military Hospital, 44 Róbert Károly Krt., 1134, Budapest, Hungary.
authors.forename: Róbert G
authors.initials: RG
authors.lastname: Kiss
authors.affiliation: Institute of Cardiology and Heart Surgery, University of Debrecen, 22 Móricz Zsigmond Krt., 4032, Debrecen, Hungary.
authors.forename: István
authors.initials: I
authors.lastname: Édes
authors.affiliation: Division of Clinical Laboratory Science, Department of Laboratory Medicine, University of Debrecen, 98 Nagyerdei Krt., 4032, Debrecen, Hungary. muszbek@med.unideb.hu.|Vascular Biology, Thrombosis and Hemostasis Research Group of the Hungarian Academy of Science, University of Debrecen, 98 Nagyerdei Krt., 4032, Debrecen, Hungary. muszbek@med.unideb.hu.
authors.forename: László
authors.identifier: http://orcid.org/0000-0002-3798-9962
authors.initials: L
authors.lastname: Muszbek
mesh_terms: D000107:Acetylation / Q000187:drug effects; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016718:Arachidonic Acid / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology* / Q000627:therapeutic use; D003324:Coronary Artery Disease / Q000188:drug therapy / Q000517:prevention & control; D051545:Cyclooxygenase 1 / Q000235:genetics / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D004351:Drug Resistance; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D008297:Male; D008875:Middle Aged; D010974:Platelet Aggregation / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013929:Thromboxane B2 / Q000096:biosynthesis
chemical_list: D012333:RNA, Messenger; D016718:Arachidonic Acid; D013929:Thromboxane B2; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; C496511:PTGS1 protein, human; C496540:PTGS2 protein, human; D001241:Aspirin
keywords: Aspirin; Coronary artery disease; Cyclooxygenase-1; Cyclooxygenase-2; Platelet; Thromboxane B2