title: Secreted expression of truncated capsid protein from porcine circovirus type 2 in Pichia pastoris.
abstract: OBJECTIVE
  To achieve secreted expression of the truncated capsid protein from porcine circovirus type 2 (PCV2) in Pichia pastoris.
  
  
  RESULTS
  A truncated cap gene (tcap) with a deleted N-terminal nuclear localization signal was optimized and synthesized. Effective secreted expression was achieved in P. pastoris GS115. The high-productive recombinant strain for tCap was grown in a 5 l bioreactor and the productivity of tCap in supernatant reached 250 μg/ml. Furthermore, serum antibody test demonstrated that adjuvant-assisting tCap induced a significant increase of specific PCV2-Cap antibody over time in mice and a similar antibody level in pigs compared with a commercial Cap-based subunit vaccine.
  
  
  CONCLUSIONS
  This work establishes a secreted expression strategy in P. pastoris for the production of PCV2 Cap with superior bioactivity, and this strategy might provide potential uses in developing Cap-based subunit vaccine in the future.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, No. 130 Meilong Road, Shanghai, 200237, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, No. 130 Meilong Road, Shanghai, 200237, China.
authors.forename: Junjun
authors.initials: J
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, No. 130 Meilong Road, Shanghai, 200237, China.|Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai, 200237, China.
authors.forename: Yuanxing
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Beijing Wanmuyuan Agriculture S&T Co., Ltd, Beijing, 100081, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhu
authors.affiliation: China Institute of Veterinary Drug Control, No. 8 Zhongguancun South Street, Beijing, 100081, China. 478264313@qq.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, No. 130 Meilong Road, Shanghai, 200237, China. qinliu@ecust.edu.cn.|Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai, 200237, China. qinliu@ecust.edu.cn.
authors.forename: Qin
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000818:Animals; D019149:Bioreactors; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D017925:Circovirus / Q000737:chemistry*; D007114:Immunization; D008807:Mice, Inbred BALB C; D010843:Pichia / Q000235:genetics*; D015202:Protein Engineering / Q000295:instrumentation / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D034421:Sus scrofa; D014765:Viral Vaccines / Q000235:genetics
chemical_list: D036022:Capsid Proteins; D011994:Recombinant Proteins; D014765:Viral Vaccines
keywords: Immunogenicity; Pichia pastoris; Porcine circovirus diseases; Porcine circovirus type 2; Secretion expression; Truncated capsid protein