title: Antimicrobial and antifouling efficacy of urinary catheters impregnated with a combination of macrolide and fluoroquinolone antibiotics against Pseudomonas aeruginosa.
abstract: The incidence of catheter associated urinary tract infections (CAUTIs) is increasing worldwide. This study was designed to modify a biomaterial by impregnating a silicone urinary catheter with combination of a macrolide, azithromycin (AZM) and a fluoroquinolone, ciprofloxacin (CIP). Drug release profiles showed slow yet continuous release of antibiotics from catheters for one month. In vitro efficacy testing showed that group B catheters [3% (w v(-1)) CIP + 6% (w v(-1)) AZM] outperformed group A catheters [2% (w v(-1)) CIP + 5% (w v(-1)) AZM] by (1) showing larger zones of inhibition (>31 mm) compared to group A (<28 mm) for up to 30 days against Pseudomonas aeruginosa PAO1; (2) killing adhered bacteria in 24 h compared to 24-48 h in group A; (3) showing longer antimicrobial durability for four weeks; and (4) exhibiting a stable real-time shelf life of one year, suggesting that these catheters can be explored in clinical settings, especially in long-term CAUTI.
authors.affiliation: a Department of Microbiology, Basic Medical Sciences Block I , Panjab University , Chandigarh , India.
authors.forename: Hina
authors.initials: H
authors.lastname: Saini
authors.affiliation: a Department of Microbiology, Basic Medical Sciences Block I , Panjab University , Chandigarh , India.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Chhibber
authors.affiliation: a Department of Microbiology, Basic Medical Sciences Block I , Panjab University , Chandigarh , India.
authors.forename: Kusum
authors.initials: K
authors.lastname: Harjai
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D017963:Azithromycin / Q000008:administration & dosage*; D018441:Biofilms / Q000187:drug effects; D055499:Catheter-Related Infections / Q000517:prevention & control*; D002939:Ciprofloxacin / Q000008:administration & dosage*; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D062885:Urinary Catheters / Q000382:microbiology*; D014552:Urinary Tract Infections / Q000517:prevention & control*
chemical_list: D000900:Anti-Bacterial Agents; D002939:Ciprofloxacin; D017963:Azithromycin
keywords: Azithromycin; Pseudomonas aeruginosa; catheter associated urinary tract infections; ciprofloxacin; urinary catheter