title: Sensorless adaptive optics implementation in widefield optical sectioning microscopy inside in vivo Drosophila brain.
abstract: We present an implementation of a sensorless adaptive optics loop in a widefield fluorescence microscope. This setup is designed to compensate for aberrations induced by the sample on both excitation and emission pathways. It allows fast optical sectioning inside a living Drosophila brain. We present a detailed characterization of the system performances. We prove that the gain brought to optical sectioning by realizing structured illumination microscopy with adaptive optics down to 50 μm deep inside living Drosophila brain.
authors.affiliation: Université Paris-Saclay, Laboratoire Aimé Cotton, CNRS, Université Paris-Sud, ENS Cachan, Orsay Cedex 91405, France.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Pedrazzani
authors.affiliation: CNRS, Laboratoire de Physique et d'Étude des Matériaux, ESPCI, 10 Rue Vauquelin, Paris 75005, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Loriette
authors.affiliation: Université Paris-Saclay, Laboratoire Aimé Cotton, CNRS, Université Paris-Sud, ENS Cachan, Orsay Cedex 91405, FrancecCNRS, Gènes et Dynamiques des Systèmes de Mémoire, Unité de Neurobiologie, ESPCI, 10 Rue Vauquelin, Paris 75005, France.
authors.forename: Paul
authors.initials: P
authors.lastname: Tchenio
authors.affiliation: Université Paris-Saclay, Laboratoire Aimé Cotton, CNRS, Université Paris-Sud, ENS Cachan, Orsay Cedex 91405, France.
authors.forename: Sakina
authors.initials: S
authors.lastname: Benrezzak
authors.affiliation: Université Paris-Saclay, Laboratoire Aimé Cotton, CNRS, Université Paris-Sud, ENS Cachan, Orsay Cedex 91405, France.
authors.forename: Daniele
authors.initials: D
authors.lastname: Nutarelli
authors.affiliation: CNRS, Laboratoire de Physique et d'Étude des Matériaux, ESPCI, 10 Rue Vauquelin, Paris 75005, France.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Fragola
mesh_terms: D000465:Algorithms; D000818:Animals; D030801:Animals, Genetically Modified; D001921:Brain / Q000000981:diagnostic imaging*; D001923:Brain Chemistry; D004330:Drosophila; D004867:Equipment Design; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008164:Luminescent Proteins / Q000737:chemistry / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D059906:Neuroimaging / Q000379:methods*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D008164:Luminescent Proteins; D011994:Recombinant Proteins