title: Effects of stimulation of muscarinic acetylcholine receptors in medial septum on some immune responses in rats.
abstract: OBJECTIVE
  Though the immunomodulatory role of medial septum (MS) has been indicated, but the contribution of the muscarinic acetylcholine (mAch) receptors presented in the internal network of the neurons of MS in this regard is not known. The aim of the present study is to assess the contribution of mAch receptors of MS on some immunological parameters.
  
  
  METHODS
  Different immunological parameters i.e. phagocytic activity of peripheral leukocytes, adhesibility and cytotoxicity of splenic mononuclear cells (MNC), delayed type of hypersensitivity (DTH) responses, Total Count (TC) and Differential Count (DC) of WBC with serum corticosterone (CORT) concentration have been measured after stimulation and blocking of mAch receptors of MS in rats. Ach or atropine has been micro infused into MS of separate groups of rats for stimulating or blocking of the mAch receptors respectively.
  
  
  RESULTS
  In Ach or atropine microinfused rats, the TC of WBC remained unaltered in the present study. The percentage of neutrophil has increased and the percentage of lymphocyte has decreased in Ach microinfused rats, but these parameters remain unaltered in atropine microinfused rats. The observed immunological parameters have increased after microinfusion of 0.60μM and 0.12μM of Ach and serum CORT concentration has decreased in those animals. These immunological parameters have decreased and serum CORT has increased after microinfusion of atropine (2 and 4mM) in MS of rats.
  
  
  CONCLUSIONS
  It appears that the Ach receptors in MS can modulate the observed immunological parameters, and serum CORT plays an important role for these immune changes.
authors.affiliation: Department of Physiology, University Colleges of Science and Technology, University of Calcutta, Kolkata, India. Electronic address: emailgdutta@gmail.com.
authors.forename: Goutam
authors.initials: G
authors.lastname: Dutta
authors.affiliation: Department of Physiology, University Colleges of Science and Technology, University of Calcutta, Kolkata, India. Electronic address: tusharkantighosh53@yahoo.in.
authors.forename: Tusharkanti
authors.initials: T
authors.lastname: Ghosh
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002448:Cell Adhesion; D003345:Corticosterone / Q000097:blood; D003602:Cytotoxicity, Immunologic; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007107:Immune System / Q000187:drug effects* / Q000502:physiology; D007962:Leukocytes / Q000187:drug effects / Q000276:immunology; D018721:Muscarinic Agonists / Q000494:pharmacology*; D018727:Muscarinic Antagonists / Q000494:pharmacology; D018377:Neurotransmitter Agents / Q000494:pharmacology*; D010587:Phagocytosis; D051381:Rats; D011976:Receptors, Muscarinic / Q000378:metabolism*; D012686:Septal Nuclei / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000276:immunology
chemical_list: D018721:Muscarinic Agonists; D018727:Muscarinic Antagonists; D018377:Neurotransmitter Agents; D011976:Receptors, Muscarinic; D001285:Atropine; D000109:Acetylcholine; D003345:Corticosterone
keywords: Immunomodulation; Medial septum; Microinfusion; mAch Receptor