title: Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants.
abstract: OBJECTIVE
  To develop and validate a microdilution method for measuring the minimum inhibitory concentration (MIC) of biosurfactants.
  
  
  RESULTS
  A standardized microdilution method including resazurin dye has been developed for measuring the MIC of biosurfactants and its validity was established through the replication of tetracycline and gentamicin MIC determination with standard bacterial strains.
  
  
  CONCLUSIONS
  This new method allows the generation of accurate MIC measurements, whilst overcoming critical issues related to colour and solubility which may interfere with growth measurements for many types of biosurfactant extracts.
authors.affiliation: School of Biomedical Sciences, Pharmaceutical Science and Practice Research Group, Ulster University, Cromore Road, Coleraine, County Londonderry, BT52 1SA, Northern Ireland, UK.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Elshikh
authors.affiliation: School of Biomedical Sciences, Pharmaceutical Science and Practice Research Group, Ulster University, Cromore Road, Coleraine, County Londonderry, BT52 1SA, Northern Ireland, UK.
authors.forename: Syed
authors.initials: S
authors.lastname: Ahmed
authors.affiliation: School of Biomedical Sciences, Pharmaceutical Science and Practice Research Group, Ulster University, Cromore Road, Coleraine, County Londonderry, BT52 1SA, Northern Ireland, UK.
authors.forename: Scott
authors.initials: S
authors.lastname: Funston
authors.affiliation: School of Biomedical Sciences, Pharmaceutical Science and Practice Research Group, Ulster University, Cromore Road, Coleraine, County Londonderry, BT52 1SA, Northern Ireland, UK.
authors.forename: Paul
authors.initials: P
authors.lastname: Dunlop
authors.affiliation: School of Biomedical Sciences, Pharmaceutical Science and Practice Research Group, Ulster University, Cromore Road, Coleraine, County Londonderry, BT52 1SA, Northern Ireland, UK.
authors.forename: Mark
authors.initials: M
authors.lastname: McGaw
authors.affiliation: School of Biomedical Sciences, Pharmaceutical Science and Practice Research Group, Ulster University, Cromore Road, Coleraine, County Londonderry, BT52 1SA, Northern Ireland, UK.
authors.forename: Roger
authors.initials: R
authors.lastname: Marchant
authors.affiliation: School of Biomedical Sciences, Pharmaceutical Science and Practice Research Group, Ulster University, Cromore Road, Coleraine, County Londonderry, BT52 1SA, Northern Ireland, UK. im.banat@ulster.ac.uk.
authors.forename: Ibrahim M
authors.identifier: http://orcid.org/0000-0001-8964-2840
authors.initials: IM
authors.lastname: Banat
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D005839:Gentamicins / Q000494:pharmacology; D007202:Indicators and Reagents; D008826:Microbial Sensitivity Tests / Q000295:instrumentation / Q000379:methods*; D010078:Oxazines; D015203:Reproducibility of Results; D013291:Streptococcus / Q000187:drug effects; D013501:Surface-Active Agents / Q000494:pharmacology*; D013752:Tetracycline / Q000494:pharmacology; D014966:Xanthenes
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D007202:Indicators and Reagents; D010078:Oxazines; D013501:Surface-Active Agents; D014966:Xanthenes; C005843:resazurin; D013752:Tetracycline
keywords: Antibiotics; Biosurfactant; Minimum inhibitory concentration; Resazurin