title: DSGOST inhibits tumor growth by blocking VEGF/VEGFR2-activated angiogenesis.
abstract: Tumor growth requires a process called angiogenesis, a new blood vessel formation from pre-existing vessels, as newly formed vessels provide tumor cells with oxygen and nutrition. Danggui-Sayuk-Ga-Osuyu-Saenggang-Tang (DSGOST), one of traditional Chinese medicines, has been widely used in treatment of vessel diseases including Raynaud's syndrome in Northeast Asian countries including China, Japan and Korea. Therefore, we hypothesized that DSGOST might inhibit tumor growth by targeting newly formed vessels on the basis of its historical prescription. Here, we demonstrate that DSGOST inhibits tumor growth by inhibiting VEGF-induced angiogenesis. DSGOST inhibited VEGF-induced angiogenic abilities of endothelial cells in vitro and in vivo, which resulted from its inhibition of VEGF/VEGFR2 interaction. Furthermore, DSGOST attenuated pancreatic tumor growth in vivo by reducing angiogenic vessel numbers, while not affecting pancreatic tumor cell viability. Thus, our data conclude that DSGOST inhibits VEGF-induced tumor angiogenesis, suggesting a new indication for DSGOST in treatment of cancer.
authors.affiliation: Department of Science in Korean Medicine, Graduate School, Kyung Hee University, 1 Hoegi, Seoul 130-701, Korea.
authors.forename: Hyeong Sim
authors.initials: HS
authors.lastname: Choi
authors.affiliation: Department of Science in Korean Medicine, Graduate School, Kyung Hee University, 1 Hoegi, Seoul 130-701, Korea.
authors.forename: Kangwook
authors.initials: K
authors.lastname: Lee
authors.affiliation: Department of Science in Korean Medicine, Graduate School, Kyung Hee University, 1 Hoegi, Seoul 130-701, Korea.
authors.forename: Min Kyoung
authors.initials: MK
authors.lastname: Kim
authors.affiliation: Department of Science in Korean Medicine, Graduate School, Kyung Hee University, 1 Hoegi, Seoul 130-701, Korea.
authors.forename: Kang Min
authors.initials: KM
authors.lastname: Lee
authors.affiliation: Department of Preventive Medicine, College of Korean Medicine, Kyung Hee University, 1 Hoegi, Seoul 130-701, Korea.
authors.forename: Yong Cheol
authors.initials: YC
authors.lastname: Shin
authors.affiliation: Department of Biotechnology, Korea National University of Transportation, Jeungpyeong, Chungbuk 368-701, Korea.
authors.forename: Sung-Gook
authors.initials: SG
authors.lastname: Cho
authors.affiliation: Department of Preventive Medicine, College of Korean Medicine, Kyung Hee University, 1 Hoegi, Seoul 130-701, Korea.
authors.forename: Seong-Gyu
authors.initials: SG
authors.lastname: Ko
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology; D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D006801:Humans; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D016328:NF-kappa B / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000517:prevention & control*; D010190:Pancreatic Neoplasms / Q000098:blood supply / Q000188:drug therapy* / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D047368:Tumor Burden / Q000187:drug effects; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism / Q000494:pharmacology*; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism*; D023041:Xenograft Model Antitumor Assays
chemical_list: D020533:Angiogenesis Inhibitors; D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; D042461:Vascular Endothelial Growth Factor A; D040301:Vascular Endothelial Growth Factor Receptor-2
keywords: DSGOST; VEGF; angiogenesis; herbal medicine; tumor