title: Calibrating Sickle Cell Disease.
abstract: Sickle cell disease is fundamentally a kinetic disorder, in which cells containing the mutated hemoglobin (hemoglobin S; HbS) will cause occlusion if they sickle in the microvasculature, but have minimal (or no) consequences if they sickle in the venous return. Physiologically, sickling always occurs when some ligands are present; nonetheless, the kinetics in the presence of ligands are virtually unstudied. Sickling arises from nucleation-controlled polymer formation, triggered when the HbS loses ligands (e.g., oxygen). Thus, understanding how nucleation responds to the presence of oxygen is the key to understanding how sickling proceeds in a physiological context. We have measured the rate of nucleus formation in HbS partially liganded with NO or CO, which we find have equivalent effects in reducing the nucleation rates. We find that hemoglobin must be in the T (tense) quaternary structure for nucleation, but the presence of ligands inhibits nucleus formation even when the correct quaternary structure is present. From these results, we can predict the fraction of cells that will sickle at any given partial ligand saturations. The ability to make such predictions may prove especially useful in designing future therapies, particularly those where the oxygen affinity is perturbed.
authors.affiliation: Department of Physics, Drexel University, Philadelphia, PA 19104, USA.
authors.forename: Donna
authors.initials: D
authors.lastname: Yosmanovich
authors.affiliation: Department of Physics, Drexel University, Philadelphia, PA 19104, USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Rotter
authors.affiliation: Department of Physics, Drexel University, Philadelphia, PA 19104, USA.
authors.forename: Alexey
authors.initials: A
authors.lastname: Aprelev
authors.affiliation: Department of Physics, Drexel University, Philadelphia, PA 19104, USA. Electronic address: fferrone@drexel.edu.
authors.forename: Frank A
authors.initials: FA
authors.lastname: Ferrone
mesh_terms: D000755:Anemia, Sickle Cell / Q000175:diagnosis* / Q000503:physiopathology*; D006451:Hemoglobin, Sickle / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry; D006801:Humans; D008024:Ligands; D008833:Microcirculation; D010100:Oxygen / Q000737:chemistry; D010101:Oxygen Consumption; D011108:Polymers / Q000737:chemistry; D011336:Probability; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary
chemical_list: D006451:Hemoglobin, Sickle; D006454:Hemoglobins; D008024:Ligands; D011108:Polymers; D010100:Oxygen
keywords: kinetics; ligands; nucleation; polymerization; therapy