title: β-lactams resistance gene quantification in an antibiotic resistant Escherichia coli water suspension treated by advanced oxidation with UV/H2O2.
abstract: Water is one of the most important habitats and route for the spread of antibiotic resistance (AR) in the environment and disinfection processes can be a potential barrier to minimise this risk. In this study the effect of UV/H2O2process on the potential of AR transfer was investigated through cultivation methods vs (polymerase chain reaction) PCR based methods. blaTEMwas selected as target antibiotic resistance gene (ARG) and was quantified by qPCR in the survived colonies and the whole suspension (total DNA). The detection limit of residual antibiotic resistant Escherichia coli (E. coli) colonies (5CFUmL-1) was reached after 240min treatment, but blaTEMgene was still present in total DNA after 300min (2.8×106copies mL-1), and no effect was observed in DNA extracted from cell cultures (3.8×108copies mL-1after 90min). Accordingly, the investigated disinfection process may select for unaffected ARGs, therefore contributing to the potential transfer of AR in the environment.
authors.affiliation: Department of Civil Engineering, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano, SA, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Ferro
authors.affiliation: Department of Chemistry and Biology, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano, SA, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Guarino
authors.affiliation: Department of Chemistry and Biology, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano, SA, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Cicatelli
authors.affiliation: Department of Civil Engineering, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano, SA, Italy. Electronic address: l.rizzo@unisa.it.
authors.forename: Luigi
authors.initials: L
authors.lastname: Rizzo
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000528:radiation effects; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000235:genetics; D004203:Disinfection; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics* / Q000528:radiation effects; D006861:Hydrogen Peroxide / Q000737:chemistry*; D010084:Oxidation-Reduction; D016133:Polymerase Chain Reaction; D014466:Ultraviolet Rays; D018440:beta-Lactam Resistance; D047090:beta-Lactams / Q000494:pharmacology* / Q000528:radiation effects
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D047090:beta-Lactams; D006861:Hydrogen Peroxide
keywords: Antibiotic resistance; DNA; Water disinfection; β- lactams resistance gene