title: Universally Conserved Relationships between Nuclear Shape and Cytoplasmic Mechanical Properties in Human Stem Cells.
abstract: The ability of cells to proliferate, differentiate, transduce extracellular signals and assemble tissues involves structural connections between nucleus and cytoskeleton. Yet, how the mechanics of these connections vary inside stem cells is not fully understood. To address those questions, we combined two-dimensional particle-tracking microrheology and morphological measures using variable reduction techniques to measure whether cytoplasmic mechanics allow for discrimination between different human adherent stem cell types and across different culture conditions. Here we show that nuclear shape is a quantifiable discriminant of mechanical properties in the perinuclear cytoskeleton (pnCSK) of various stem cell types. Also, we find the pnCSK is a region with different mechanical properties than elsewhere in the cytoskeleton, with heterogeneously distributed locations exhibiting subdiffusive features, and which obeys physical relations conserved among various stem cell types. Finally, we offer a prospective basis to discriminate between stem cell types by coupling perinuclear mechanical properties to nuclear shape.
authors.affiliation: Epigenetics and Stem Cell Biology Laboratory, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA.
authors.forename: Oswaldo A
authors.initials: OA
authors.lastname: Lozoya
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Gilchrist
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.|Departments of Orthopaedic Surgery, Biomedical Engineering, and Developmental Biology, Washington University in St. Louis, St. Louis, MO 63110, USA.|Shriners Hospitals for Children - St. Louis, St. Louis, MO 63110, USA.
authors.forename: Farshid
authors.initials: F
authors.lastname: Guilak
mesh_terms: D000199:Actins / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology; D000704:Analysis of Variance; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism / Q000502:physiology*; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism / Q000502:physiology*; D003599:Cytoskeleton / Q000378:metabolism / Q000502:physiology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D018613:Microscopy, Confocal; D011446:Prospective Studies; D012212:Rheology / Q000379:methods; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D059008:Time-Lapse Imaging / Q000379:methods
chemical_list: D000199:Actins