title: Fasciola gigantica enolase is a major component of worm tegumental fraction protective against sheep fasciolosis.
abstract: Infection of cattle and sheep with the parasite Fasciola gigantica is a cause of important economic losses throughout Asia and Africa. Many of the available anthelmintics have undesirable side effects, and the parasite may acquire drug resistance as a result of mass and repeated treatments of livestock. Accordingly, the need for developing a vaccine is evident. Triton-soluble surface membrane and tegumental proteins (TSMTP) of 60, 32, and 28 kDa previously shown to elicit protective immunity in mice against challenge F. gigantica infection were found to be strongly immunogenic in sheep eliciting vigorous specific antibody responses to a titer>1:16,000 as assessed by enzyme-linked immunosorbent assay. Furthermore, the 60 kDa fraction induced production of antibodies able to bind to the surface membrane of newly excysted juvenile flukes and mediate their attrition in antibody-dependent complement- and cell-mediated cytotoxicity assays, and significant (P<0.05) 40% protection of sheep against F. gigantica challenge infection. Amino acid micro sequencing of the 60 kDa-derived tryptic peptides revealed the fraction predominantly consists of F. gigantica enolase. The cDNA nucleotide and translated amino acid sequences of F. gigantica enolase showed homology of 92% and 95%, respectively to Fasciola hepatica enolase, suggesting that a fasciolosis vaccine might be effective against both tropical and temperate liver flukes.
authors.affiliation: Zoology Department, Faculty of Science, Cairo University, Cairo 12613, Egypt.
authors.forename: N
authors.initials: N
authors.lastname: Mahana
authors.affiliation: Zoology Department, Faculty of Science, Cairo University, Cairo 12613, Egypt.
authors.forename: H A-S
authors.initials: HA
authors.lastname: Abd-Allah
authors.affiliation: Schistosoma Biological Materials Supply Program, Theodore Bilharz Research Institute, Giza 12411, Egypt.
authors.forename: M
authors.initials: M
authors.lastname: Salah
authors.affiliation: Department of Chemistry, School of Science and Engineering, American University in Cairo, New Cairo 11835, Cairo, Egypt.
authors.forename: H
authors.initials: H
authors.lastname: Tallima
authors.affiliation: Zoology Department, Faculty of Science, Cairo University, Cairo 12613, Egypt. Electronic address: rashikaelridi@hotmail.com.
authors.forename: R
authors.initials: R
authors.lastname: El Ridi
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D005209:Fasciola / Q000201:enzymology / Q000276:immunology*; D005211:Fascioliasis / Q000517:prevention & control / Q000662:veterinary*; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010751:Phosphopyruvate Hydratase / Q000276:immunology* / Q000378:metabolism; D012756:Sheep; D012757:Sheep Diseases / Q000517:prevention & control*; D014612:Vaccines / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D014612:Vaccines; D010751:Phosphopyruvate Hydratase
keywords: Enolase; Fasciola gigantica; Fasciolosis; Vaccination