title: Effects of overexpression of PKAc genes on expressions of lignin-modifying enzymes by Pleurotus ostreatus.
abstract: We studied the role of genes encoding the cAMP-dependent protein kinase A catalytic subunit (PKAc) in the ligninolytic system in Pleurotus ostreatus. The wild-type P. ostreatus strain PC9 has two PKAc-encoding genes: PKAc1 and PKAc2 (protein ID 114122 and 85056). In the current study, PKAc1 and PKAc2 were fused with a β-tubulin promoter and introduced into strain PC9 to produce the overexpression strains PKAc1-97 and PKAc2-69. These strains showed significantly higher transcription levels of isozyme genes encoding lignin-modifying enzymes than strain PC9, but the specific gene expression patterns differed between the two recombinant strains. Both recombinants showed 2.05-2.10-fold faster degradation of beechwood lignin than strain PC9. These results indicate that PKAc plays an important role in inducing the wood degradation system in P. ostreatus.
authors.affiliation: a School of Environmental Science , University of Shiga Prefecture , Hikone , Japan.
authors.forename: Chihana
authors.initials: C
authors.lastname: Toyokawa
authors.affiliation: a School of Environmental Science , University of Shiga Prefecture , Hikone , Japan.
authors.forename: Misaki
authors.initials: M
authors.lastname: Shobu
authors.affiliation: a School of Environmental Science , University of Shiga Prefecture , Hikone , Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Tsukamoto
authors.affiliation: a School of Environmental Science , University of Shiga Prefecture , Hikone , Japan.
authors.forename: Saki
authors.initials: S
authors.lastname: Okamura
authors.affiliation: b Graduate School of Agriculture , Kyoto University , Kyoto , Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Honda
authors.affiliation: c Gifu Prefectural Research Institute for Forests , Gifu , Japan.
authors.forename: Hisatoshi
authors.initials: H
authors.lastname: Kamitsuji
authors.affiliation: a School of Environmental Science , University of Shiga Prefecture , Hikone , Japan.
authors.forename: Kousuke
authors.initials: K
authors.lastname: Izumitsu
authors.affiliation: a School of Environmental Science , University of Shiga Prefecture , Hikone , Japan.
authors.forename: Kazumi
authors.initials: K
authors.lastname: Suzuki
authors.affiliation: a School of Environmental Science , University of Shiga Prefecture , Hikone , Japan.
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Irie
mesh_terms: D001673:Biodegradation, Environmental; D054751:Cyclic AMP-Dependent Protein Kinase Catalytic Subunits / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D007527:Isoenzymes / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D008031:Lignin / Q000737:chemistry*; D020076:Pleurotus / Q000201:enzymology* / Q000235:genetics
chemical_list: D007527:Isoenzymes; D008031:Lignin; D054751:Cyclic AMP-Dependent Protein Kinase Catalytic Subunits; C463959:protein kinase A Calpha
keywords: holocellulose; mushroom; signal transduction; white-rot