title: Predictive Value of Plasma MicroRNA-216a/b in the Diagnosis of Esophageal Squamous Cell Carcinoma.
abstract: Esophageal squamous cell carcinoma (ESCC) is a common human malignancy with poor survival, which was usually diagnosed at an advanced stage. MicroRNAs (miRNAs), a class of single stranded noncoding RNAs with only 17-25 ribonucleotides, were demonstrated to play an important role in lots of cancers. In the recent years, increasing evidence revealed that circulating miRNAs exhibited great potential in the diagnosis of various types of cancers. The present study was designed to evaluate the diagnostic value of plasma miRNA-216a/b for ESCC. Our results showed that the expression level of plasma miRNA-216a/b was significantly lower in ESCC patients compared with that of healthy controls. The receiver operating characteristic (ROC) curve analysis yielded an area under the ROC curve (AUC) value of 0.877 [95% CI (confidence interval): 0.818-0.922] for miRNA-216a and 0.756 (95% CI: 0.685-0.819) for miRNA-216b. Clinical data indicated that plasma miRNA-216a/b were inversely correlated with lymph node metastasis and TNM stage. Additionally, the plasma miRNA-216b expression level was significantly upregulated in postoperative samples compared to preoperative samples. Our study, for the first time, demonstrated that plasma miRNA-216a/b might serve as potential biomarkers for the diagnosis of ESCC and dysregulation of miRNA-216a/b might be involved in the progression of ESCC.
authors.affiliation: Department of Medical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China; Key Medical Laboratory of Henan Province, Zhengzhou, Henan 450052, China.
authors.forename: Shuling
authors.initials: S
authors.lastname: Dong
authors.affiliation: Department of Medical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China; Key Medical Laboratory of Henan Province, Zhengzhou, Henan 450052, China.
authors.forename: Huiqing
authors.initials: H
authors.lastname: Yin
authors.affiliation: Department of Medical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China; Key Medical Laboratory of Henan Province, Zhengzhou, Henan 450052, China.
authors.forename: Cuicui
authors.initials: C
authors.lastname: Dong
authors.affiliation: Department of Medical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China; Key Medical Laboratory of Henan Province, Zhengzhou, Henan 450052, China.
authors.forename: Kaiyan
authors.initials: K
authors.lastname: Sun
authors.affiliation: Department of Medical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China; Key Medical Laboratory of Henan Province, Zhengzhou, Henan 450052, China.
authors.forename: Pin
authors.initials: P
authors.lastname: Lv
authors.affiliation: Department of Medical Laboratory, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Meng
authors.affiliation: Department of Medical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China; Key Medical Laboratory of Henan Province, Zhengzhou, Henan 450052, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Ming
authors.affiliation: Department of Medical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China; Key Medical Laboratory of Henan Province, Zhengzhou, Henan 450052, China.
authors.forename: Fucheng
authors.initials: F
authors.lastname: He
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D015536:Down-Regulation; D055088:Early Detection of Cancer; D004938:Esophageal Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D011237:Predictive Value of Tests; D012372:ROC Curve
chemical_list: D014408:Biomarkers, Tumor; C575482:MIRN216 microRNA, human; D035683:MicroRNAs