title: Pattern of infection and antibiotic activity among Streptococcus agalactiae isolates from adults in Mashhad, Iran.
abstract: BACKGROUND
  One of the main causes of sexually transmitted diseases is group B β- hemolytic streptococci (GBS) multiplying in the genital tracts. Penicillin is the most common drug for the treatment of infections caused by these bacteria, but in patients suffering from Penicillin allergy, Erythromycin and Clindamycin are used as alternative therapeutic drugs against GBS. Recently, resistance to these drugs has been reported more often. In this study, efforts have been made to determine the prevalence and antibiotic resistance of GBS.
  
  
  METHODS
  Modified Christie Atkins Munch-Petersen (CAMP) test was conducted on over 2400 samples of urine and discharge taken from vagina, urethra and prostate. The drug sensitivity was performed by double disk sensitivity tests to Bacitracin, Trimethoprim, and Sulfamethoxazole and then the resistant samples were investigated by E-test to determine the minimal inhibitory concentrations (MICs) value.
  
  
  RESULTS
  Twenty-three vaginal and 10 urethral discharge, 27urine and 6 prostatic secretion samples were GBS positive. The most symbiotic microorganisms with GBS were strains of Enterococci (90%), Staphylococcus saprophyticus (25%) and Candida albicans (6%). The disk diffusion method showed 18 cases with Penicillin resistance (MIC: 1.5 mg/ml).
  
  
  CONCLUSIONS
  Taken together, GBS carriers' rate in this study was found 20.65% (8.24% men and 12.4% women). Furthermore, findings showed high-level resistance to Erythromycin and Clindamycin.
authors.affiliation: Department of Laboratory Medicine, School of Paramedicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Masoumeh
authors.initials: M
authors.lastname: Malek-Jafarian
authors.affiliation: Department of Laboratory Medicine, School of Paramedicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Fatemeh-Sadat
authors.initials: FS
authors.lastname: Hosseini
authors.affiliation: Department of Laboratory Medicine, School of Paramedicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Abodol-Reza
authors.initials: AR
authors.lastname: Ahmadi
keywords: Antibiotic resistance; Genitourinary system; Streptococcus agalactiae; minimal inhibitory concentration (MIC)