title: Mesangiogenic Progenitor Cells Derived from One Novel CD64(bright)CD31(bright)CD14(neg) Population in Human Adult Bone Marrow.
abstract: Mesenchymal stromal cells (MSCs) have been the object of extensive research for decades, due to their intrinsic clinical value. Nonetheless, the unambiguous identification of a unique in vivo MSC progenitor is still lacking, and the hypothesis that these multipotent cells could possibly arise from different in vivo precursors has been gaining consensus in the last years. We identified a novel multipotent cell population in human adult bone marrow that we first named Mesodermal Progenitor Cells (MPCs) for the ability to differentiate toward the mesenchymal lineage, while still retaining angiogenic potential. Despite extensive characterization, MPCs positioning within the differentiation pathway and whether they can be ascribed as possible distinctive progenitor of the MSC lineage is still unclear. In this study, we describe the ex vivo isolation of one novel bone marrow subpopulation (Pop#8) with the ability to generate MPCs. Multicolor flow cytometry in combination with either fluorescence-activated cell sorting or magnetic-activated cell sorting were applied to characterize Pop#8 as CD64(bright)CD31(bright)CD14(neg). We defined Pop#8 properties in culture, including the potential of Pop#8-derived MPCs to differentiate into MSCs. Gene expression data were suggestive of Pop#8 in vivo involvement in hematopoietic stem cell niche constitution/maintenance. Pop#8 resulted over three logs more frequent than other putative MSC progenitors, corroborating the idea that most of the controversies regarding culture-expanded MSCs could be the consequence of different culture conditions that select or promote particular subpopulations of precursors.
authors.affiliation: 1 Hematology Division, Department of Clinical and Experimental Medicine, University of Pisa , Pisa, Italy .
authors.forename: Simone
authors.initials: S
authors.lastname: Pacini
authors.affiliation: 1 Hematology Division, Department of Clinical and Experimental Medicine, University of Pisa , Pisa, Italy .
authors.forename: Serena
authors.initials: S
authors.lastname: Barachini
authors.affiliation: 1 Hematology Division, Department of Clinical and Experimental Medicine, University of Pisa , Pisa, Italy .
authors.forename: Marina
authors.initials: M
authors.lastname: Montali
authors.affiliation: 2 Department of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of Pisa , Pisa, Italy .
authors.forename: Vittoria
authors.initials: V
authors.lastname: Carnicelli
authors.affiliation: 1 Hematology Division, Department of Clinical and Experimental Medicine, University of Pisa , Pisa, Italy .
authors.forename: Rita
authors.initials: R
authors.lastname: Fazzi
authors.affiliation: 3 First Orthopedic Division, Department of Translational Research and New Technology in Medicine and Surgery, University of Pisa , Pisa, Italy .
authors.forename: Paolo
authors.initials: P
authors.lastname: Parchi
authors.affiliation: 1 Hematology Division, Department of Clinical and Experimental Medicine, University of Pisa , Pisa, Italy .
authors.forename: Mario
authors.initials: M
authors.lastname: Petrini
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology*; D019070:Cell Lineage; D002469:Cell Separation / Q000379:methods*; D048430:Cell Shape; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D008648:Mesoderm / Q000166:cytology*; D008875:Middle Aged; D018919:Neovascularization, Physiologic; D013234:Stem Cells / Q000166:cytology*
chemical_list: D015703:Antigens, CD