title: Development and validation of a method to determine thiabendazole and o-phenylphenol in wastewater using micellar liquid chromatography-fluorescence detection.
abstract: A micellar liquid chromatographic method to determine thiabendazole (TBZ) and o-phenylphenol in wastewater is described here. The sample was directly injected without any additional treatment other filtration. The pesticides were resolved in <11 min, using a mobile phase of 0.10 M SDS-6% 1-pentanol buffered at pH 3 running through a C18 column at 1 mL/min. The detection was performed by fluorescence at 305/360 and 245/345 nm excitation/emission wavelengths for TBZ and o-phenylphenol, respectively. The method was validated following the directives of the Validation and Peer Review of U.S. Environmental Protection Agency Chemical Methods of Analysis guidelines in terms of selectivity, quantitation range (0.01-0.02 to 2 mg/L), detection limit (0.005-0.008 mg/L), trueness (92.1-104.2%), precision (<13.9%), robustness (<6.6%), and stability under storage conditions. The procedure was applied to the screening of TBZ and o-phenylphenol in wastewater samples from citrus packing plants, agricultural gutters, urban sewage, as well as in influent and effluent wastewater treatment plants.
authors.affiliation: Departament de Química Física i Analítica, ESTCE, Universitat Jaume I, Castelló, Spain. vicentej@uji.es.
authors.forename: Juan
authors.initials: J
authors.lastname: Peris-Vicente
authors.affiliation: Departament de Química Física i Analítica, ESTCE, Universitat Jaume I, Castelló, Spain.
authors.forename: Pasqual
authors.initials: P
authors.lastname: Roca-Genovés
authors.affiliation: Departament de Química Física i Analítica, ESTCE, Universitat Jaume I, Castelló, Spain.
authors.forename: Khaled
authors.initials: K
authors.lastname: Tayeb-Cherif
authors.affiliation: Departament de Química Física i Analítica, ESTCE, Universitat Jaume I, Castelló, Spain.
authors.forename: Josep
authors.initials: J
authors.lastname: Esteve-Romero
mesh_terms: D000383:Agriculture; D001713:Biphenyl Compounds / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D002853:Chromatography, Liquid / Q000379:methods*; D002957:Citrus; D016018:Least-Squares Analysis; D057230:Limit of Detection; D008823:Micelles; D010573:Pesticide Residues / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D013827:Thiabendazole / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D062065:Wastewater / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D001713:Biphenyl Compounds; D008823:Micelles; D010573:Pesticide Residues; D013827:Thiabendazole; D062065:Wastewater; D014874:Water Pollutants, Chemical; C004369:2-phenylphenol
keywords: Direct injection; Micellar; Pesticides; Validation; Wastewater