title: Effects of dietary supplementation with epidermal growth factor-expressing Saccharomyces cerevisiae on duodenal development in weaned piglets.
abstract: The aim of the present study was to assess the effects of dietary supplementation with epidermal growth factor (EGF)-expressing Saccharomyces cerevisiae on duodenal development in weaned piglets. In total, forty piglets weaned at 21-26 d of age were assigned to one of the five groups that were provided basic diet (control group) or diet supplemented with S. cerevisiae expressing either empty-vector (INVSc1(EV) group), tagged EGF (T-EGF) (INVSc1-TE(-) group), extracellular EGF (EE-EGF) (INVSc1-EE(+) group) or intracellular EGF (IE-EGF) (INVSc1-IE(+) group). All treatments were delivered as 60·00 μg/kg body weight EGF/d. On 0, 7, 14 and 21 d, eight piglets per treatment were sacrificed to analyse the morphology, activities and mRNA expressions of digestive enzymes, as well as Ig levels (IgA, IgM, IgG) in duodenal mucosa. The results showed significant improvement on 7, 14 and 21 d, with respect to average daily gain (P<0·05), mucosa morphology (villus height and crypt depth) (P<0·05), Ig levels (P<0·01), activities and mRNA expressions of digestive enzymes (creatine kinase, alkaline phosphatase, lactate dehydrogenase and sucrase) (P<0·05) and the mRNA expression of EGF-receptor (P<0·01) in NVSc1-TE(-), INVSc1-EE(+) and INVSc1-IE(+) groups compared with control and INVSc1(EV) groups. In addition, a trend was observed in which the INVSc1-IE(+) group showed an improvement in Ig levels (0·05<P<0·10), mRNA expressions of digestive enzymes and EGF-receptor (P<0·05) compared with NVSc1-TE(-) and INVSc1-EE(+) groups. These results indicate that supplementing recombinant EGF-expressing S. cerevisiae to the diet of weaned piglets enhanced duodenal development. Moreover, biological activity (Ig levels, mRNA expressions of digestive enzymes and EGF-receptor) of IE-EGF was better than either EE-EGF or T-EGF.
authors.affiliation: 1College of Life Science and Technology,Southwest University for Nationalities,Chengdu 610041,People's Republic of China.
authors.forename: Shujin
authors.initials: S
authors.lastname: Wang
authors.affiliation: 1College of Life Science and Technology,Southwest University for Nationalities,Chengdu 610041,People's Republic of China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Guo
authors.affiliation: 3Shenzhen Premix Inve Nutrition Co. Ltd,Shenzhen518103,People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhou
authors.affiliation: 4Chengdu LiLai Biotechnology Co. Ltd,Chengdu610041,People's Republic of China.
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Zhong
authors.affiliation: 1College of Life Science and Technology,Southwest University for Nationalities,Chengdu 610041,People's Republic of China.
authors.forename: Wuzheng
authors.initials: W
authors.lastname: Zhu
authors.affiliation: 1College of Life Science and Technology,Southwest University for Nationalities,Chengdu 610041,People's Republic of China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Huang
authors.affiliation: 1College of Life Science and Technology,Southwest University for Nationalities,Chengdu 610041,People's Republic of China.
authors.forename: Zhengfan
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: 2Maastricht University Medical Center+,University Eye Clinic Maastricht,6229 ER Maastricht,The Netherlands.
authors.forename: Theo G M F
authors.initials: TG
authors.lastname: Gorgels
authors.affiliation: 2Maastricht University Medical Center+,University Eye Clinic Maastricht,6229 ER Maastricht,The Netherlands.
authors.forename: Tos T J M
authors.initials: TT
authors.lastname: Berendschot
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D003402:Creatine Kinase / Q000235:genetics / Q000378:metabolism; D019587:Dietary Supplements; D004386:Duodenum / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000008:administration & dosage / Q000494:pharmacology*; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism; D007136:Immunoglobulins / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D013294:Lactococcus lactis; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013393:Sucrase / Q000235:genetics / Q000378:metabolism; D013552:Swine; D014886:Weaning
chemical_list: D007136:Immunoglobulins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D004815:Epidermal Growth Factor; D007770:L-Lactate Dehydrogenase; D066246:ErbB Receptors; D003402:Creatine Kinase; D000469:Alkaline Phosphatase; D013393:Sucrase
keywords: ALP alkaline phosphatase; Administration forms of epidermal growth factor; BW body weight; CK creatine kinase; Duodenal development; EE-EGF extracellular expressing epidermal growth factor; EGF epidermal growth factor; EGF-R epidermal growth factor receptor; GIT gastrointestinal tract; IE-EGF intracellular expressing epidermal growth factor; INVSc1(EV) empty vector-expressing-Saccharomyces cerevisiae; INVSc1-EE(+) EE-EGF-expressing-Saccharomyces cerevisiae; INVSc1-IE(+) IE-EGF-expressing-Saccharomyces cerevisiae; INVSc1-TE(−) T-EGF protein-expressing-Saccharomyces cerevisiae; LDH lactate dehydrogenase; T-EGF tagged epidermal growth factor; Weaned piglets; Weaning stress