title: Magnetic solid lipid nanoparticles in hyperthermia against colon cancer.
abstract: A reproducible double emulsion/solvent evaporation procedure is developed to formulate magnetic solid lipid nanoparticles (average size≈180 nm) made of iron oxide cores embedded within a glyceryl trimyristate solid matrix. The physicochemical characterization of the nanocomposites ascertained the efficacy of the preparation conditions in their production, i.e. surface properties (electrokinetic and thermodynamic data) were almost indistinguishable from those of the solid lipid nanomatrix, while electron microscopy characterizations and X-ray diffraction patterns confirmed the satisfactory coverage of the magnetite nuclei. Hemocompatibility of the particles was established in vitro. Hysteresis cycle determinations defined the appropriate magnetic responsiveness of the nanocomposites, and their heating characteristics were investigated in a high frequency alternating gradient of magnetic field: a constant maximum temperature of 46 °C was obtained within 40 min. Finally, in vitro tests performed on human HT29 colon adenocarcinoma cells demonstrated a promising decrease in cell viability after treatment with the nanocomposites and exposure to that alternating electromagnetic field. To the best of our knowledge, this is the first time that such type of nanoformulation with very promising hyperthermia characteristics has been developed for therapeutic aims.
authors.affiliation: Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Granada, Granada, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Muñoz de Escalona
authors.affiliation: Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Granada, Granada, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Sáez-Fernández
authors.affiliation: Institute of Biopathology and Regenerative Medicine (IBIMER), University of Granada, Granada, Spain; Biosanitary Institute of Granada (ibs.GRANADA), Andalusian Health Service (SAS)-University of Granada, Granada, Spain.
authors.forename: José C
authors.initials: JC
authors.lastname: Prados
authors.affiliation: Institute of Biopathology and Regenerative Medicine (IBIMER), University of Granada, Granada, Spain; Biosanitary Institute of Granada (ibs.GRANADA), Andalusian Health Service (SAS)-University of Granada, Granada, Spain.
authors.forename: Consolación
authors.initials: C
authors.lastname: Melguizo
authors.affiliation: Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Granada, Granada, Spain; Institute of Biopathology and Regenerative Medicine (IBIMER), University of Granada, Granada, Spain; Biosanitary Institute of Granada (ibs.GRANADA), Andalusian Health Service (SAS)-University of Granada, Granada, Spain. Electronic address: jlarias@ugr.es.
authors.forename: José L
authors.initials: JL
authors.lastname: Arias
mesh_terms: D000328:Adult; D001777:Blood Coagulation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms / Q000628:therapy*; D005260:Female; D019073:HT29 Cells; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D053761:Nanocomposites / Q000008:administration & dosage* / Q000737:chemistry; D015539:Platelet Activation / Q000187:drug effects; D014280:Triglycerides / Q000737:chemistry; D055815:Young Adult
chemical_list: D058185:Magnetite Nanoparticles; D014280:Triglycerides; C036694:trimyristin
keywords: Cancer therapy; Colon cancer; Hemocompatibility; Magnetic colloid; Magnetic fluid hyperthermia; Magnetic solid lipid nanoparticle; Nanocomposite