title: MALDI mass spectrometry imaging of N-glycans on tibial cartilage and subchondral bone proteins in knee osteoarthritis.
abstract: Magnetic resonance imaging (MRI) is a non-invasive technique routinely used to investigate pathological changes in knee osteoarthritis (OA) patients. MRI uniquely reveals zones of the most severe change in the subchondral bone (SCB) in OA, called bone marrow lesions (BMLs). BMLs have diagnostic and prognostic significance in OA, but MRI does not provide a molecular understanding of BMLs. Multiple N-glycan structures have been observed to play a pivotal role in the OA disease process. We applied matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) of N-glycans to formalin-fixed paraffin-embedded (FFPE) SCB tissue sections from patients with knee OA, and liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) was conducted on consecutive sections to structurally characterize and correlate with the N-glycans seen by MALDI-MSI. The application of this novel MALDI-MSI protocol has enabled the first steps to spatially investigate the N-glycome in the SCB of knee OA patients.
authors.affiliation: Adelaide Proteomics Centre, School of Biological Sciences, University of Adelaide, Adelaide, South Australia, Australia.|Institute of Photonics and Advanced Sensing (IPAS), University of Adelaide, Adelaide, South Australia, Australia.
authors.forename: Matthew T
authors.initials: MT
authors.lastname: Briggs
authors.affiliation: Discipline of Orthopaedics and Trauma, School of Medicine, University of Adelaide, Adelaide, South Australia, Australia.|Bone and Joint Research Laboratory, SA Pathology, Adelaide, South Australia, Australia.
authors.forename: Julia S
authors.initials: JS
authors.lastname: Kuliwaba
authors.affiliation: Discipline of Orthopaedics and Trauma, School of Medicine, University of Adelaide, Adelaide, South Australia, Australia.|Bone and Joint Research Laboratory, SA Pathology, Adelaide, South Australia, Australia.
authors.forename: Dzenita
authors.initials: D
authors.lastname: Muratovic
authors.affiliation: Biomolecular Frontiers Research Centre, Faculty of Science, Macquarie University, Sydney, New South Wales, Australia.|Centre of Excellence for Nanoscale BioPhotonics (CNBP), Macquarie University, Sydney, New South Wales, Australia.
authors.forename: Arun V
authors.initials: AV
authors.lastname: Everest-Dass
authors.affiliation: Biomolecular Frontiers Research Centre, Faculty of Science, Macquarie University, Sydney, New South Wales, Australia.|Centre of Excellence for Nanoscale BioPhotonics (CNBP), Macquarie University, Sydney, New South Wales, Australia.
authors.forename: Nicolle H
authors.initials: NH
authors.lastname: Packer
authors.affiliation: Discipline of Orthopaedics and Trauma, School of Medicine, University of Adelaide, Adelaide, South Australia, Australia.
authors.forename: David M
authors.initials: DM
authors.lastname: Findlay
authors.affiliation: Adelaide Proteomics Centre, School of Biological Sciences, University of Adelaide, Adelaide, South Australia, Australia.|Institute of Photonics and Advanced Sensing (IPAS), University of Adelaide, Adelaide, South Australia, Australia.
authors.forename: Peter
authors.initials: P
authors.lastname: Hoffmann
mesh_terms: D000368:Aged; D001853:Bone Marrow / Q000473:pathology; D002356:Cartilage / Q000737:chemistry / Q000000981:diagnostic imaging* / Q000473:pathology; D002853:Chromatography, Liquid / Q000379:methods; D005260:Female; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D008875:Middle Aged; D057054:Molecular Imaging / Q000379:methods; D020370:Osteoarthritis, Knee / Q000175:diagnosis / Q000000981:diagnostic imaging* / Q000473:pathology; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013977:Tibia / Q000000981:diagnostic imaging / Q000473:pathology
chemical_list: D011134:Polysaccharides
keywords: Bone marrow lesion; Glycans; Glycoproteomics; Maldi imaging; Mass spectrometry; Osteoarthritis