title: Anti-trypanosomal compound, sagamilactam, a new polyene macrocyclic lactam from Actinomadura sp. K13-0306.
abstract: A new antibiotic, designated sagamilactam, was isolated from the cultured broth of Actinomadura sp. K13-0306 by Diaion HP-20, silica gel and octadecylsilane column chromatography followed by purification by HPLC. The chemical structure of the new compound was elucidated by spectroscopic analyses, including NMR and MS. The structure of sagamilactam proved to be a new compound consisting of a polyunsaturated and polyoxygenated 34-membered macrocyclic lactam containing diene, triene and tetraene conjugated olefins and a decalin moiety. Sagamilactam showed antitrypanosomal activity with an IC50value of 0.25±0.11 μM.
authors.affiliation: Department of Drug Discovery Sciences, Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Tōru
authors.initials: T
authors.lastname: Kimura
authors.affiliation: Department of Drug Discovery Sciences, Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.|Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Iwatsuki
authors.affiliation: Department of Drug Discovery Sciences, Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.|Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Asami
authors.affiliation: Department of Drug Discovery Sciences, Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.|Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Aki
authors.initials: A
authors.lastname: Ishiyama
authors.affiliation: Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Rei
authors.initials: R
authors.lastname: Hokari
authors.affiliation: Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Otoguro
authors.affiliation: Department of Drug Discovery Sciences, Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.|Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Matsumoto
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Sato
authors.affiliation: Department of Drug Discovery Sciences, Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.|Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Kazuro
authors.initials: K
authors.lastname: Shiomi
authors.affiliation: Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Yōko
authors.initials: Y
authors.lastname: Takahashi
authors.affiliation: Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ōmura
authors.affiliation: Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Takuji
authors.initials: T
authors.lastname: Nakashima
mesh_terms: D000192:Actinomycetales / Q000737:chemistry*; D000475:Alkenes / Q000737:chemistry / Q000494:pharmacology; D000981:Antiprotozoal Agents / Q000737:chemistry / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D047029:Lactams, Macrocyclic / Q000737:chemistry / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D008970:Molecular Weight; D011090:Polyenes / Q000737:chemistry / Q000494:pharmacology*; D014346:Trypanosoma brucei brucei / Q000187:drug effects
chemical_list: D000475:Alkenes; D000981:Antiprotozoal Agents; D047029:Lactams, Macrocyclic; D011090:Polyenes