title: Isoflavone supplementation in postmenopausal women does not affect leukocyte LDL receptor and scavenger receptor CD36 expression: A double-blind, randomized, placebo-controlled trial.
abstract: Isoflavones are discussed to improve serum lipoproteins and body composition and to reduce cardiovascular disease risk in postmenopausal women (PMW). LDL receptors (LDLR) and scavenger receptor CD36 (CD36) play a pivotal role in the regulation of plasma LDL-cholesterol concentrations (LDL-chol). We investigated the impact of isoflavones on the receptor expression of both receptors in leukocytes of PMW.
  
  
  
  A randomized, double-blind, placebo-controlled trial in parallel design was conducted to assess the effects of an isoflavone-enriched soy extract (117.4 mg/day isoflavone aglycone equivalents) for 12 weeks on serum LDL-chol, LDLR, and CD36 expression on leukocytes in 170 healthy PMW. Baseline and after 12 weeks, blood lipid concentrations, anthropometric data and body composition were determined. Receptor expression on leukocytes was measured by means of flow cytometry. After the intervention, no significant differences were found for LDLR and CD36 expression on leukocytes. A significant increase of serum LDL-chol was shown for the isoflavone group (p = 0.03) after 12 weeks. Body fat content and VAT were not affected.
  
  
  
  Isoflavone supplementation for 12 weeks did not change LDLR and CD36 expression on leukocytes of PMW and did not affect body fat content and visceral adipose tissue (VAT), but slightly increased serum LDL-chol.
authors.affiliation: Department of Physiology and Biochemistry of Nutrition, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Karlsruhe, Germany.
authors.forename: Ann Katrin
authors.initials: AK
authors.lastname: Engelbert
authors.affiliation: Department of Safety and Quality of Fruit and Vegetables, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Karlsruhe, Germany.
authors.forename: Sebastian T
authors.initials: ST
authors.lastname: Soukup
authors.affiliation: Department of Physiology and Biochemistry of Nutrition, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Karlsruhe, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Roth
authors.affiliation: Department of Physiology and Biochemistry of Nutrition, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Karlsruhe, Germany.
authors.forename: Nadine
authors.initials: N
authors.lastname: Hoffmann
authors.affiliation: Department of Physiology and Biochemistry of Nutrition, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Karlsruhe, Germany.
authors.forename: Daniela
authors.initials: D
authors.lastname: Graf
authors.affiliation: Department of Physiology and Biochemistry of Nutrition, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Karlsruhe, Germany.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Watzl
authors.affiliation: Department of Safety and Quality of Fruit and Vegetables, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Karlsruhe, Germany.
authors.forename: Sabine E
authors.initials: SE
authors.lastname: Kulling
authors.affiliation: Department of Physiology and Biochemistry of Nutrition, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Karlsruhe, Germany. Achim.Bub@mri.bund.de.
authors.forename: Achim
authors.initials: A
authors.lastname: Bub
mesh_terms: D000368:Aged; D001823:Body Composition / Q000187:drug effects; D018955:CD36 Antigens / Q000097:blood*; D019587:Dietary Supplements; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism; D008055:Lipids / Q000097:blood; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D008875:Middle Aged; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D017698:Postmenopause / Q000187:drug effects; D011973:Receptors, LDL / Q000097:blood*; D013025:Glycine max / Q000737:chemistry
chemical_list: D018955:CD36 Antigens; D007529:Isoflavones; D008055:Lipids; D011973:Receptors, LDL
keywords: Isoflavones; LDL cholesterol; LDL receptor; postmenopausal women; visceral adipose tissue