title: B cells establish, but do not maintain, long-lived murine anti-peanut IgE(a).
abstract: BACKGROUND
  Peanut allergy (PNA) has been reported to be transferred to tolerant recipients through organ and bone marrow (BM) transplantation. The roles T and B cells play in establishing, and the roles B cell subsets play in maintaining lifelong anti-peanut IgE levels are unknown.
  
  
  OBJECTIVE
  To determine the cellular requirements for the transfer of murine PNA and to determine the role CD20(+) cells play in maintaining long-lived anti-peanut IgE levels.
  
  
  METHODS
  We developed a novel adoptive transfer model to investigate the cellular requirements for transferring murine PNA. We also treated peanut-allergic (PA) mice with anti-CD20 antibody and measured IgE levels throughout treatment.
  
  
  RESULTS
  Purified B220(+) cells from PA splenocytes and purified CD4(+) cells from naïve (NA) splenocytes are the minimal requirements for the adoptive transfer of PNA. Prolonged treatment of allergic mice with anti-CD20 antibody results in significant depletion of B cell subsets but does not affect anti-peanut IgE levels, symptoms, or numbers of IgE antibody secreting cells (ASCs) in the BM. Adoptive transfer of BM and spleen cells from allergic donors treated with anti-CD20 antibody does not result in the transfer of PNA in NA recipients, demonstrating that anti-CD20 antibody treatment depletes B cells capable of differentiating into peanut-specific IgE ASCs.
  
  
  CONCLUSIONS
  Peanut allergy can be established in a NA hosts with B220(+) cells from PA donors and CD4(+) cells from peanut-NA donors. However, long-term depletion of B220(+) cells with anti-CD20 antibody does not affect anti-peanut IgE levels. These results highlight a novel role for B cells in the development of PNA and provide evidence that long-lived anti-peanut IgE levels may be maintained by long-lived ASCs.
authors.affiliation: Division of Allergy and Clinical Immunology, Departments of Medicine and Immunology, University of Colorado Denver, Aurora, CO, USA.
authors.forename: D M
authors.identifier: http://orcid.org/0000-0002-4071-3756
authors.initials: DM
authors.lastname: Moutsoglou
authors.affiliation: Division of Allergy and Clinical Immunology, Departments of Medicine and Immunology, University of Colorado Denver, Aurora, CO, USA.
authors.forename: S C
authors.initials: SC
authors.lastname: Dreskin
mesh_terms: D019264:Adoptive Transfer; D000485:Allergens / Q000276:immunology*; D000818:Animals; D010367:Arachis / Q000009:adverse effects*; D016175:B-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D015415:Biomarkers; D018932:Chemokine CCL2 / Q000378:metabolism; D004195:Disease Models, Animal; D007073:Immunoglobulin E / Q000276:immunology*; D016130:Immunophenotyping; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008407:Mast Cells / Q000276:immunology / Q000378:metabolism; D051379:Mice; D021183:Peanut Hypersensitivity / Q000276:immunology* / Q000378:metabolism
chemical_list: D000485:Allergens; D015415:Biomarkers; D018932:Chemokine CCL2; D007073:Immunoglobulin E; D017493:Leukocyte Common Antigens
keywords: IgE; adoptive transfer; anaphylaxis; antibody secreting cell; memory B cell