title: High level soluble expression and one-step purification of IBDV VP2 protein in Escherichia coli.
abstract: OBJECTIVE
  To improve the expression of soluble IBDV VP2 protein by using different tagged vectors in Escherichia coli.
  
  
  RESULTS
  Fusion tags, Grifin, MBP, SUMO, thioredoxin, γ-crystallin, ArsC and PpiB, enhanced the expression and solubility of VP2 protein. The fusion proteins were purified by Ni-NTA chromatography, MBP-VP2 showed the highest purity about 90 %. After removing the MBP tag, VP2 self-assembled into virus-like particles, ~25 nm diam. Results from AGP suggested the recombinant IBDV VP2 protein identified by reference serum like IBDV.
  
  
  CONCLUSIONS
  All the seven tags enhanced the expression and solubility of IBDV VP2 protein. The recombinant protein self-assembly into virus like particles and possess antigenicity as reference IBDV.
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.|Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Jiang
authors.affiliation: Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China.
authors.forename: Yunchao
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Life Sciences, Zhengzhou University, Zhengzhou, 450001, People's Republic of China.
authors.forename: Aiping
authors.initials: A
authors.lastname: Wang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China. zhanggaiping2003@163.com.|Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China. zhanggaiping2003@163.com.
authors.forename: Gaiping
authors.initials: G
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.
authors.forename: Guoyu
authors.initials: G
authors.lastname: Yang
authors.affiliation: Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China.|School of Life Sciences, Zhengzhou University, Zhengzhou, 450001, People's Republic of China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China.
authors.forename: Pengchao
authors.initials: P
authors.lastname: Ji
authors.affiliation: Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.|Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China.
authors.forename: Yapeng
authors.initials: Y
authors.lastname: Song
authors.affiliation: Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China.
authors.forename: Yunfang
authors.initials: Y
authors.lastname: Su
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.|Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Wang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.|Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China.
authors.forename: Jucai
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.|Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China.
authors.forename: Baolei
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China.
authors.forename: Ruiguang
authors.initials: R
authors.lastname: Deng
mesh_terms: D000818:Animals; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D007106:Immune Sera; D047468:Immunoprecipitation; D008854:Microscopy, Electron / Q000379:methods; D015202:Protein Engineering / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D012995:Solubility; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D007106:Immune Sera; D011993:Recombinant Fusion Proteins; C082513:VP2 protein, infectious bursal disease virus; D015678:Viral Structural Proteins
keywords: Capsid protein; Fusion protein; Infectious bursal disease; Infectious bursal disease virus; Protein expression; Vaccine