title: Directed Assembly and Development of Material-Free Tissues with Complex Architectures.
abstract: Material-free tissues are assembled using solely cells. Microstructured hydrogel templates and high content screening allow the formation of centimeter-scale tissues with precise architectures. Similar to developing tissues, these contract autonomously, controllably shift shape, self-scaffold by secreting extracellular matrix, and undergo morphogenesis.
authors.affiliation: Department of Complex Tissue Regeneration, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229, ER, Maastricht, The Netherland.
authors.forename: Erik
authors.initials: E
authors.lastname: Vrij
authors.affiliation: Department of Biomechanical Engineering, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Drienerlolaan 5, 7522, NB, Enschede, The Netherlands.
authors.forename: Jeroen
authors.initials: J
authors.lastname: Rouwkema
authors.affiliation: Department of Complex Tissue Regeneration, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229, ER, Maastricht, The Netherland.
authors.forename: Vanessa
authors.initials: V
authors.lastname: LaPointe
authors.affiliation: Department of Complex Tissue Regeneration, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229, ER, Maastricht, The Netherland.
authors.forename: Clemens
authors.initials: C
authors.lastname: van Blitterswijk
authors.affiliation: Department of Complex Tissue Regeneration, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229, ER, Maastricht, The Netherland.
authors.forename: Roman
authors.initials: R
authors.lastname: Truckenmüller
authors.affiliation: Department of Complex Tissue Regeneration, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229, ER, Maastricht, The Netherland.|Hubrecht Institute for Developmental Biology and Stem Cell Research, Uppsalalaan 8, 3584, CT, Utrecht, The Netherlands.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Rivron
mesh_terms: D005109:Extracellular Matrix; D020100:Hydrogels; D023822:Tissue Engineering
chemical_list: D020100:Hydrogels
keywords: bottom-up tissue engineering; cellular building blocks; directed development; high-throughput screening; tissues