title: Positioning of centrioles is a conserved readout of Frizzled planar cell polarity signalling.
abstract: Planar cell polarity (PCP) signalling is a well-conserved developmental pathway regulating cellular orientation during development. An evolutionarily conserved pathway readout is not established and, moreover, it is thought that PCP mediated cellular responses are tissue-specific. A key PCP function in vertebrates is to regulate coordinated centriole/cilia positioning, a function that has not been associated with PCP in Drosophila. Here we report instructive input of Frizzled-PCP (Fz/PCP) signalling into polarized centriole positioning in Drosophila wings. We show that centrioles are polarized in pupal wing cells as a readout of PCP signalling, with both gain and loss-of-function Fz/PCP signalling affecting centriole polarization. Importantly, loss or gain of centrioles does not affect Fz/PCP establishment, implicating centriolar positioning as a conserved PCP-readout, likely downstream of PCP-regulated actin polymerization. Together with vertebrate data, these results suggest a unifying model of centriole/cilia positioning as a common downstream effect of PCP signalling from flies to mammals.
authors.affiliation: Department of Developmental &Regenerative Biology, Tisch Cancer Institute, and Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, New York, New York 10029, USA.
authors.forename: Jose Maria
authors.initials: JM
authors.lastname: Carvajal-Gonzalez
authors.affiliation: Champalimaud Neuroscience Programme, Avenida de Brasilia, Lisbon 1400-038, Portugal.
authors.forename: Angel-Carlos
authors.initials: AC
authors.lastname: Roman
authors.affiliation: Department of Developmental &Regenerative Biology, Tisch Cancer Institute, and Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, New York, New York 10029, USA.
authors.forename: Marek
authors.initials: M
authors.lastname: Mlodzik
mesh_terms: D000818:Animals; D016764:Cell Polarity; D002502:Centrioles / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster; D051157:Frizzled Receptors / Q000378:metabolism*; D011679:Pupa / Q000166:cytology; D015398:Signal Transduction; D014921:Wings, Animal / Q000166:cytology
chemical_list: D029721:Drosophila Proteins; D051157:Frizzled Receptors; C058784:fz protein, Drosophila