title: Rotational manipulation of single cells and organisms using acoustic waves.
abstract: The precise rotational manipulation of single cells or organisms is invaluable to many applications in biology, chemistry, physics and medicine. In this article, we describe an acoustic-based, on-chip manipulation method that can rotate single microparticles, cells and organisms. To achieve this, we trapped microbubbles within predefined sidewall microcavities inside a microchannel. In an acoustic field, trapped microbubbles were driven into oscillatory motion generating steady microvortices which were utilized to precisely rotate colloids, cells and entire organisms (that is, C. elegans). We have tested the capabilities of our method by analysing reproductive system pathologies and nervous system morphology in C. elegans. Using our device, we revealed the underlying abnormal cell fusion causing defective vulval morphology in mutant worms. Our acoustofluidic rotational manipulation (ARM) technique is an easy-to-use, compact, and biocompatible method, permitting rotation regardless of optical, magnetic or electrical properties of the sample under investigation.
authors.affiliation: Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Ahmed
authors.affiliation: Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
authors.forename: Adem
authors.initials: A
authors.lastname: Ozcelik
authors.affiliation: Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
authors.forename: Nagagireesh
authors.initials: N
authors.lastname: Bojanala
authors.affiliation: Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
authors.forename: Nitesh
authors.initials: N
authors.lastname: Nama
authors.affiliation: Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
authors.forename: Awani
authors.initials: A
authors.lastname: Upadhyay
authors.affiliation: Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
authors.forename: Yuchao
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
authors.forename: Wendy
authors.identifier: http://orcid.org/0000-0002-5566-4758
authors.initials: W
authors.lastname: Hanna-Rose
authors.affiliation: Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.|Department of Bioengineering, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
authors.forename: Tony Jun
authors.initials: TJ
authors.lastname: Huang
mesh_terms: D000162:Acoustics / Q000295:instrumentation; D000818:Animals; D017173:Caenorhabditis elegans; D004867:Equipment Design; D006367:HeLa Cells; D006801:Humans; D046210:Microfluidic Analytical Techniques; D008846:Micromanipulation / Q000295:instrumentation / Q000379:methods*; D012399:Rotation; D013016:Sound