title: MiR-30a-5p/UBE3C axis regulates breast cancer cell proliferation and migration.
abstract: Aberrant expression of ubiquitin Protein ligase E3C (UBE3C) has been documented in breast cancer (BC). MicroRNAs (miRNAs) were shown to play an important role in the regulation of tumor properties in BC. However, whether miRNAs contributes to UBE3C expression in BC cells remains poorly understood. In this study, we report that UBE3C was a direct target of miR-30a-5p. Expression of miR-30a-5p in BC cells reduced UBE3C expression. MCF-7 and MDA-MB-453 cells were transfected miR-30a-5p-overexpression, and found that cell proliferation and migration were inhibited. In contrast, when miR-30a-5p inhibitor were transfected into MCF-7 and MDA-MB-453 cells, cell proliferation and migration were promoted. We study demonstrated that upregulation of miR-30a-5p was significantly suppressed levels of cyclin B1, cyclin D1 and c-myc. Moreover, Correlation analysis indicated that expression of miR-30a-5p was highly negatively correlated with UBE3C, which was upregulated in BC specimens. These data highlight the important role of miR-30a-5p/UBE3C axis in BC development and progression. Therefore, miR-30a-5p activation or UBE3C inhibition may be provide a novel strategy for the treatment of BC.
authors.affiliation: Basic Medical College, Inner Mongolia Medical University, Hohhot, People's Republic of China; Beijing Institute of Radiation Medicine, Beijing, People's Republic of China.
authors.forename: Jiaxiu
authors.initials: J
authors.lastname: Xiong
authors.affiliation: Department of General Surgery, Chinese PLA General Hospital, Beijing, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wei
authors.affiliation: Beijing Institute of Radiation Medicine, Beijing, People's Republic of China.
authors.forename: Qinong
authors.initials: Q
authors.lastname: Ye
authors.affiliation: Shenhua Shendong General Hospital, Yulin, People's Republic of China. Electronic address: lwz19682002@163.com.
authors.forename: Wenzhong
authors.initials: W
authors.lastname: Liu
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D044767:Ubiquitin-Protein Ligases / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C546086:MIRN30b microRNA, human; D035683:MicroRNAs; C555759:UBE3C protein, human; D044767:Ubiquitin-Protein Ligases
keywords: Breast cancer; MiR-30a-5p; Proliferation and migration; UBE3C; miRNA