title: Hydrophilic CeO2 nanocubes protect pancreatic β-cell line INS-1 from H2O2-induced oxidative stress.
abstract: Oxidative stress plays a key role in the occurrence and development of diabetes. With their unique redox properties, CeO2 nanoparticles (nanoceria) exhibit promising potential for the treatment of diabetes resulting from oxidative stress. Here, we develop a novel preparation of hydrophilic CeO2 nanocubes (NCs) with two different sizes (5 nm and 25 nm) via an acetate assisted hydrothermal method. Dynamic light scattering, zeta potential measurements and thermogravimetric analyses were utilized to investigate the changes in the physico-chemical characteristics of CeO2 NCs when exposed to in vitro cell culture conditions. CCK-8 assays revealed that the CeO2 NCs did not impair cell proliferation in the pancreatic β-cell line INS-1 at the highest dose of 200 μg mL(-1) over the time scale of 72 h, while being able to protect INS-1 cells from H2O2-induced cytotoxicity even after protein adsorption. It is also noteworthy that nanoceria with a smaller hydrodynamic radius exhibit stronger antioxidant and anti-apoptotic effects, which is consistent with their H2O2 quenching capability in biological systems. These findings suggest that nanoceria can be used as an excellent antioxidant for controlling oxidative stress-induced pancreatic β-cell damage.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Rare Earth Materials Chemistry and Applications, PKU-HKU Joint Laboratory in Rare Earth Materials and Bioinorganic Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. sun@pku.edu.cn yan@pku.edu.cn.
authors.forename: Guang-Ming
authors.initials: GM
authors.lastname: Lyu
authors.affiliation: Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Rare Earth Materials Chemistry and Applications, PKU-HKU Joint Laboratory in Rare Earth Materials and Bioinorganic Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. sun@pku.edu.cn yan@pku.edu.cn and Key Laboratory for Advanced Battery Materials and System (MOE), School of Materials Science and Engineering, Huazhong University of Science and Technology (HUST), Wuhan, Hubei 430074, China.
authors.forename: Yan-Jie
authors.initials: YJ
authors.lastname: Wang
authors.affiliation: Department of Endocrinology, 306 Hospital of People's Liberation Army, Beijing 100101, China. damaoermaosanmao@aliyun.com.
authors.forename: Xue
authors.initials: X
authors.lastname: Huang
authors.affiliation: Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Rare Earth Materials Chemistry and Applications, PKU-HKU Joint Laboratory in Rare Earth Materials and Bioinorganic Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. sun@pku.edu.cn yan@pku.edu.cn.
authors.forename: Huai-Yuan
authors.initials: HY
authors.lastname: Zhang
authors.affiliation: Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Rare Earth Materials Chemistry and Applications, PKU-HKU Joint Laboratory in Rare Earth Materials and Bioinorganic Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. sun@pku.edu.cn yan@pku.edu.cn.
authors.forename: Ling-Dong
authors.initials: LD
authors.lastname: Sun
authors.affiliation: Department of Endocrinology, 306 Hospital of People's Liberation Army, Beijing 100101, China. damaoermaosanmao@aliyun.com.
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Liu
authors.affiliation: Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Rare Earth Materials Chemistry and Applications, PKU-HKU Joint Laboratory in Rare Earth Materials and Bioinorganic Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. sun@pku.edu.cn yan@pku.edu.cn.
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Yan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002563:Cerium / Q000008:administration & dosage / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D057927:Hydrophobic and Hydrophilic Interactions; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats
chemical_list: D000975:Antioxidants; D002563:Cerium; C030583:ceric oxide; D006861:Hydrogen Peroxide