title: Chronic methamphetamine exposure prior to middle cerebral artery occlusion increases infarct volume and worsens cognitive injury in Male mice.
abstract: Emerging evidence indicates that methamphetamine (MA) abuse can impact cardiovascular disease. In humans, MA abuse is associated with an increased risk of stroke as well as an earlier age at which the stroke occurs. However, little is known about how chronic daily MA exposure can impact ischemic outcome in either humans or animal models. In the present study, mice were injected with MA (10 mg/kg, i.p.) or saline once daily for 10 consecutive days. Twenty-four hours after the final injection, mice were subjected to transient middle cerebral artery occlusion (tMCAO) for one hour followed by reperfusion. Mice were tested for novel object memory at 96 h post-reperfusion, just prior to removal of brains for quantification of infarct volume using 2,3,5-Triphenyltetrazolium Chloride (TTC) staining. Mice treated with MA prior to tMCAO showed decreased object memory recognition and increased infarct volume compared to saline-treated mice. These findings indicate that chronic MA exposure can worsen both cognitive and morphological outcomes following cerebral ischemia.
authors.affiliation: Department of Behavioral Neuroscience, Oregon Health and Science University, 3181 SW S Jackson Pk Rd, Portland, OR, 97239, USA. dzuloaga@albany.edu.|Department of Psychology, University at Albany, SUNY, 1400 Washington Ave, Albany, NY, 12222, USA. dzuloaga@albany.edu.
authors.forename: Damian G
authors.initials: DG
authors.lastname: Zuloaga
authors.affiliation: Division of Neuroscience, Oregon National Primate Research Center, Oregon Health and Science University, Portland, OR, 97239, USA.
authors.forename: Jianming
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Behavioral Neuroscience, Oregon Health and Science University, 3181 SW S Jackson Pk Rd, Portland, OR, 97239, USA.
authors.forename: Sydney
authors.initials: S
authors.lastname: Weber
authors.affiliation: Department of Behavioral Neuroscience, Oregon Health and Science University, 3181 SW S Jackson Pk Rd, Portland, OR, 97239, USA.
authors.forename: Gregory P
authors.initials: GP
authors.lastname: Mark
authors.affiliation: Department of Behavioral Neuroscience, Oregon Health and Science University, 3181 SW S Jackson Pk Rd, Portland, OR, 97239, USA.|Department of Anesthesiology and Perioperative Medicine, Oregon Health and Science University, Portland, OR, 97239, USA.
authors.forename: Stephanie J
authors.initials: SJ
authors.lastname: Murphy
authors.affiliation: Department of Behavioral Neuroscience, Oregon Health and Science University, 3181 SW S Jackson Pk Rd, Portland, OR, 97239, USA. raberj@ohsu.edu.|Departments of Neurology and Radiation Medicine, Oregon Health and Science University, Portland, OR, 97239, USA. raberj@ohsu.edu.|Division of Neuroscience, Oregon National Primate Research Center, Oregon Health and Science University, Portland, OR, 97239, USA. raberj@ohsu.edu.
authors.forename: Jacob
authors.initials: J
authors.lastname: Raber
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology; D001921:Brain / Q000187:drug effects / Q000473:pathology*; D000697:Central Nervous System Stimulants / Q000008:administration & dosage*; D003071:Cognition / Q000187:drug effects* / Q000502:physiology; D003072:Cognition Disorders / Q000473:pathology*; D004195:Disease Models, Animal; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology*; D008297:Male; D008568:Memory / Q000187:drug effects* / Q000502:physiology; D008694:Methamphetamine / Q000008:administration & dosage*; D051379:Mice
chemical_list: D000697:Central Nervous System Stimulants; D008694:Methamphetamine
keywords: Methamphetamine; cognition; memory; middle cerebral artery occlusion