title: Validation of a Multiplex Real-Time PCR Assay for Detection of Mycobacterium spp., Mycobacterium tuberculosis Complex, and Mycobacterium avium Complex Directly from Clinical Samples by Use of the BD Max Open System.
abstract: A multiplex real-time PCR was validated on the BD Max open system to detect different Mycobacterium tuberculosis complex, Mycobacterium avium complex, and Mycobacterium spp. directly from clinical samples. The PCR results were compared to those with traditional cultures. The multiplex PCR assay was found to be a specific and sensitive method for the rapid detection of mycobacteria directly from clinical specimens.
authors.affiliation: Esoteric Testing Laboratory, Pathology Department, Tampa General Hospital, Tampa, Florida, USA.|Laboratório Especial de Microbiologia Clínica (LEMC), Federal University of São Paulo/UNIFESP, São Paulo, Brazil.
authors.forename: Talita T
authors.initials: TT
authors.lastname: Rocchetti
authors.affiliation: Esoteric Testing Laboratory, Pathology Department, Tampa General Hospital, Tampa, Florida, USA ssilbert@tgh.org.
authors.forename: Suzane
authors.initials: S
authors.lastname: Silbert
authors.affiliation: Esoteric Testing Laboratory, Pathology Department, Tampa General Hospital, Tampa, Florida, USA.
authors.forename: Alicia
authors.initials: A
authors.lastname: Gostnell
authors.affiliation: Esoteric Testing Laboratory, Pathology Department, Tampa General Hospital, Tampa, Florida, USA.
authors.forename: Carly
authors.initials: C
authors.lastname: Kubasek
authors.affiliation: Esoteric Testing Laboratory, Pathology Department, Tampa General Hospital, Tampa, Florida, USA.
authors.forename: Raymond
authors.initials: R
authors.lastname: Widen
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D009165:Mycobacterium Infections, Nontuberculous / Q000175:diagnosis; D015269:Mycobacterium avium Complex / Q000235:genetics / Q000302:isolation & purification*; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D009170:Nontuberculous Mycobacteria / Q000235:genetics / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D014376:Tuberculosis / Q000175:diagnosis