title: MiR-128-2 inhibits common lymphoid progenitors from developing into progenitor B cells.
abstract: A considerable number of studies revealed that B cell development is finely regulated by transcription factors (TFs). Recent studies suggested that TFs are coordinated with microRNAs to control the development of B cells in numerous checkpoints. In the present study, we first found that miR-128-2 was differentially expressed in various immune organs and immunocytes. B cell development was inhibited in miR-128-2-overexpressed chimera and transgenic (TG) mice in bone marrow with decreased preproB, preB, proB, immature B, and recirculating B cells, as well as increased common lymphoid progenitors (CLPs). Further experiments showed that the apoptosis of CLP decreased, but proliferation was not altered in miR-128-2-overexpressed mice. Extensive studies suggested that the inhibition of apoptosis of CLP may be caused by miR-128-2 targeting A2B and MALT1, thereby increasing the phosphorylation of ERK and P38 MAPK. Such findings have prompted future investigations on the function of miR-128-2 in lymph genesis.
authors.affiliation: Institutes of Biology and Medical Sciences, Soochow University, Suzhou, Jiangsu Province, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Institutes of Biology and Medical Sciences, Soochow University, Suzhou, Jiangsu Province, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.affiliation: Institutes of Biology and Medical Sciences, Soochow University, Suzhou, Jiangsu Province, People's Republic of China.
authors.forename: Huo
authors.initials: H
authors.lastname: Chen
authors.affiliation: Institutes of Biology and Medical Sciences, Soochow University, Suzhou, Jiangsu Province, People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Fei
authors.affiliation: Institutes of Biology and Medical Sciences, Soochow University, Suzhou, Jiangsu Province, People's Republic of China.
authors.forename: YuXu
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Institutes of Biology and Medical Sciences, Soochow University, Suzhou, Jiangsu Province, People's Republic of China.
authors.forename: Yunqiu
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Institutes of Biology and Medical Sciences, Soochow University, Suzhou, Jiangsu Province, People's Republic of China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Institutes of Biology and Medical Sciences, Soochow University, Suzhou, Jiangsu Province, People's Republic of China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology; D020169:Caspases / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D054503:Lymphoid Progenitor Cells / Q000166:cytology* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D000074884:Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein; D009363:Neoplasm Proteins / Q000378:metabolism; D010766:Phosphorylation; D054448:Precursor Cells, B-Lymphoid / Q000166:cytology* / Q000276:immunology; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D035683:MicroRNAs; C550324:Mirn128 microRNA, mouse; D009363:Neoplasm Proteins; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D020169:Caspases; C498509:Malt1 protein, mouse; D000074884:Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein
keywords: B cell development; CLP; Immune response; Immunity; Immunology and Microbiology Section; apoptosis; miR-128-2