title: Early short-term treatment with neutralizing human monoclonal antibodies halts SHIV infection in infant macaques.
abstract: Prevention of mother-to-child transmission (MTCT) of HIV remains a major objective where antenatal care is not readily accessible. We tested HIV-1-specific human neutralizing monoclonal antibodies (NmAbs) as a post-exposure therapy in an infant macaque model for intrapartum MTCT. One-month-old rhesus macaques were inoculated orally with the simian-human immunodeficiency virus SHIVSF162P3. On days 1, 4, 7 and 10 after virus exposure, we injected animals subcutaneously with NmAbs and quantified systemic distribution of NmAbs in multiple tissues within 24 h after antibody administration. Replicating virus was found in multiple tissues by day 1 in animals that were not treated. All NmAb-treated macaques were free of virus in blood and tissues at 6 months after exposure. We detected no anti-SHIV T cell responses in blood or tissues at necropsy, and no virus emerged after CD8(+) T cell depletion. These results suggest that early passive immunotherapy can eliminate early viral foci and thereby prevent the establishment of viral reservoirs.
authors.affiliation: Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.|Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USA.
authors.forename: Ann J
authors.initials: AJ
authors.lastname: Hessell
authors.affiliation: Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
authors.forename: J Pablo
authors.identifier: http://orcid.org/0000-0002-1311-060X
authors.initials: JP
authors.lastname: Jaworski
authors.affiliation: Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
authors.forename: Erin
authors.initials: E
authors.lastname: Epson
authors.affiliation: Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), Bethesda, Maryland, USA.
authors.forename: Kenta
authors.initials: K
authors.lastname: Matsuda
authors.affiliation: Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
authors.forename: Shilpi
authors.initials: S
authors.lastname: Pandey
authors.affiliation: Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
authors.forename: Christoph
authors.initials: C
authors.lastname: Kahl
authors.affiliation: Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USA.
authors.forename: Jason
authors.initials: J
authors.lastname: Reed
authors.affiliation: Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
authors.forename: William F
authors.initials: WF
authors.lastname: Sutton
authors.affiliation: Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USA.
authors.forename: Katherine B
authors.initials: KB
authors.lastname: Hammond
authors.affiliation: Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
authors.forename: Tracy A
authors.initials: TA
authors.lastname: Cheever
authors.affiliation: Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
authors.forename: Philip T
authors.initials: PT
authors.lastname: Barnette
authors.affiliation: Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
authors.forename: Alfred W
authors.initials: AW
authors.lastname: Legasse
authors.affiliation: Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
authors.forename: Shannon
authors.initials: S
authors.lastname: Planer
authors.affiliation: Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: Stanton
authors.affiliation: Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Amarendra
authors.initials: A
authors.lastname: Pegu
authors.affiliation: Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Chen
authors.affiliation: Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Keyun
authors.initials: K
authors.lastname: Wang
authors.affiliation: Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
authors.forename: Don
authors.initials: D
authors.lastname: Siess
authors.affiliation: Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
authors.forename: David
authors.initials: D
authors.lastname: Burke
authors.affiliation: Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
authors.forename: Byung S
authors.initials: BS
authors.lastname: Park
authors.affiliation: Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.|Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USA.
authors.forename: Michael K
authors.initials: MK
authors.lastname: Axthelm
authors.affiliation: Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
authors.forename: Anne
authors.initials: A
authors.lastname: Lewis
authors.affiliation: Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), Bethesda, Maryland, USA.
authors.forename: Vanessa M
authors.initials: VM
authors.lastname: Hirsch
authors.affiliation: Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Barney S
authors.initials: BS
authors.lastname: Graham
authors.affiliation: Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: John R
authors.initials: JR
authors.lastname: Mascola
authors.affiliation: Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.|Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USA.
authors.forename: Jonah B
authors.initials: JB
authors.lastname: Sacha
authors.affiliation: Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.|Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USA.
authors.forename: Nancy L
authors.initials: NL
authors.lastname: Haigwood
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage* / Q000276:immunology; D057134:Antibodies, Neutralizing / Q000008:administration & dosage* / Q000276:immunology; D004195:Disease Models, Animal; D015658:HIV Infections / Q000188:drug therapy / Q000635:transmission / Q000821:virology; D015497:HIV-1 / Q000276:immunology / Q000472:pathogenicity; D006801:Humans; D008251:Macaca / Q000821:virology; D009034:Mother-Child Relations; D016097:Simian Acquired Immunodeficiency Syndrome / Q000188:drug therapy* / Q000276:immunology / Q000635:transmission / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000187:drug effects* / Q000276:immunology / Q000472:pathogenicity
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing