title: Whole genome sequence analysis of unidentified genetically modified papaya for development of a specific detection method.
abstract: Identification of transgenic sequences in an unknown genetically modified (GM) papaya (Carica papaya L.) by whole genome sequence analysis was demonstrated. Whole genome sequence data were generated for a GM-positive fresh papaya fruit commodity detected in monitoring using real-time polymerase chain reaction (PCR). The sequences obtained were mapped against an open database for papaya genome sequence. Transgenic construct- and event-specific sequences were identified as a GM papaya developed to resist infection from a Papaya ringspot virus. Based on the transgenic sequences, a specific real-time PCR detection method for GM papaya applicable to various food commodities was developed. Whole genome sequence analysis enabled identifying unknown transgenic construct- and event-specific sequences in GM papaya and development of a reliable method for detecting them in papaya food commodities.
authors.affiliation: National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan. Electronic address: kosnakamura@nihs.go.jp.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Nakamura
authors.affiliation: National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan. Electronic address: kondo@nihs.go.jp.
authors.forename: Kazunari
authors.initials: K
authors.lastname: Kondo
authors.affiliation: National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Akiyama
authors.affiliation: National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Ishigaki
authors.affiliation: National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Noguchi
authors.affiliation: FASMAC CO., LTD, 5-1-3 Midorigaoka, Atsugi, Kanagawa 243-0041, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Katsumata
authors.affiliation: FASMAC CO., LTD, 5-1-3 Midorigaoka, Atsugi, Kanagawa 243-0041, Japan.
authors.forename: Kazuto
authors.initials: K
authors.lastname: Takasaki
authors.affiliation: FASMAC CO., LTD, 5-1-3 Midorigaoka, Atsugi, Kanagawa 243-0041, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Futo
authors.affiliation: National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Kozue
authors.initials: K
authors.lastname: Sakata
authors.affiliation: National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Nozomi
authors.initials: N
authors.lastname: Fukuda
authors.affiliation: Analytical Science Division, National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Mano
authors.affiliation: Analytical Science Division, National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
authors.forename: Kazumi
authors.initials: K
authors.lastname: Kitta
authors.affiliation: Graduate School of Agriculture, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Tanaka
authors.affiliation: Graduate School of Agriculture, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Akashi
authors.affiliation: National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Nishimaki-Mogami
mesh_terms: D029441:Carica / Q000737:chemistry / Q000235:genetics*; D005638:Fruit / Q000737:chemistry / Q000235:genetics*; D023281:Genomics; D030821:Plants, Genetically Modified / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D017421:Sequence Analysis / Q000379:methods*
keywords: Carica papaya L.; Genetically modified; Genome sequence; Next-generation sequencer; Real-time PCR; Transgenic sequence