title: GENETIC ADAPTATION AND ACCLIMATION OF PHYTOPLANKTON ALONG A STRESS GRADIENT IN THE EXTREME WATERS OF THE AGRIO RIVER-CAVIAHUE LAKE (ARGENTINA)(1).
abstract: We tested if different adaptation strategies were linked to a stress gradient in phytoplankton cells. For this purpose, we studied the adaptation and acclimation of Dictyosphaerium chlorelloides (Naumann) Komárek et Perman (Chlorophyta) and Microcystis aeruginosa (Kütz.) Kütz. (Cyanobacteria) to different water samples (from extremely acid, metal-rich water to moderate stressful conditions) of the Agrio River-Caviahue Lake system (Neuquén, Argentina). Both experimental strains were isolated from pristine, slightly alkaline waters. To distinguish between physiological acclimation and genetic adaptation (an adaptive evolution event), a modified Luria-Delbrück fluctuation analysis was carried out with both species by using as selective agent sample waters from different points along the stress gradient. M. aeruginosa did not acclimate to any of the waters tested from different points along the stress gradient nor did D. chlorelloides to the two most acidic and metal-rich waters. However, D. chlorelloides proliferated by rapid genetic adaptation, as the consequence of a single mutation (5.4 × 10(-7) resistant mutants per cell per division) at one locus, in less extreme water and also by acclimation in the least extreme water. It is hypothesized that the stress gradient resulted in different strategies of adaptation in phytoplankton cells from nonextreme waters. Thus, very extreme conditions were lethal for both organisms, but as stressful conditions decreased, adaptation of D. chlorelloides cells was possible by the selection of resistant mutants, and in less extreme conditions, by acclimation.
authors.affiliation: Departamento de Producción Animal (Genética), Facultad de Veterinaria, Universidad Complutense, E-28040 Madrid, SpainDepartamento de Toxicología y Farmacología, Facultad de Veterinaria, Universidad Complutense, E-28040 Madrid, SpainDepartamento de Biología Vegetal (Botánica), Facultad de Ciencias, Universidad de Málaga, Campus de Teatinos s/n, E-29071 Málaga, SpainDepartamento de Producción Animal (Genética), Facultad de Veterinaria, Universidad Complutense, E-28040 Madrid, Spain.
authors.forename: Victoria
authors.initials: V
authors.lastname: López-Rodas
authors.affiliation: Departamento de Producción Animal (Genética), Facultad de Veterinaria, Universidad Complutense, E-28040 Madrid, SpainDepartamento de Toxicología y Farmacología, Facultad de Veterinaria, Universidad Complutense, E-28040 Madrid, SpainDepartamento de Biología Vegetal (Botánica), Facultad de Ciencias, Universidad de Málaga, Campus de Teatinos s/n, E-29071 Málaga, SpainDepartamento de Producción Animal (Genética), Facultad de Veterinaria, Universidad Complutense, E-28040 Madrid, Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: Rouco
authors.affiliation: Departamento de Producción Animal (Genética), Facultad de Veterinaria, Universidad Complutense, E-28040 Madrid, SpainDepartamento de Toxicología y Farmacología, Facultad de Veterinaria, Universidad Complutense, E-28040 Madrid, SpainDepartamento de Biología Vegetal (Botánica), Facultad de Ciencias, Universidad de Málaga, Campus de Teatinos s/n, E-29071 Málaga, SpainDepartamento de Producción Animal (Genética), Facultad de Veterinaria, Universidad Complutense, E-28040 Madrid, Spain.
authors.forename: Sebastián
authors.initials: S
authors.lastname: Sánchez-Fortún
authors.affiliation: Departamento de Producción Animal (Genética), Facultad de Veterinaria, Universidad Complutense, E-28040 Madrid, SpainDepartamento de Toxicología y Farmacología, Facultad de Veterinaria, Universidad Complutense, E-28040 Madrid, SpainDepartamento de Biología Vegetal (Botánica), Facultad de Ciencias, Universidad de Málaga, Campus de Teatinos s/n, E-29071 Málaga, SpainDepartamento de Producción Animal (Genética), Facultad de Veterinaria, Universidad Complutense, E-28040 Madrid, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Flores-Moya
authors.affiliation: Departamento de Producción Animal (Genética), Facultad de Veterinaria, Universidad Complutense, E-28040 Madrid, SpainDepartamento de Toxicología y Farmacología, Facultad de Veterinaria, Universidad Complutense, E-28040 Madrid, SpainDepartamento de Biología Vegetal (Botánica), Facultad de Ciencias, Universidad de Málaga, Campus de Teatinos s/n, E-29071 Málaga, SpainDepartamento de Producción Animal (Genética), Facultad de Veterinaria, Universidad Complutense, E-28040 Madrid, Spain.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Costas
keywords: Dictyosphaerium; Microcystis; acclimation; adaptation; extreme environments; mutation