title: Dual-Quantum-Dots-Labeled Lateral Flow Strip Rapidly Quantifies Procalcitonin and C-reactive Protein.
abstract: In the article, a dual-quantum-dots-labeled (dual-QDs-labeled) lateral flow strip (LFS) method was developed for the simultaneous and rapid quantitative detection of procalcitonin (PCT) and C-reactive protein (CRP) in the blood. Two QD-antibody conjugates with different fluorescence emission spectra were produced and sprayed on the LFS to capture PCT and CRP in the blood. Furthermore, a double antibody sandwich method for PCT and, meanwhile, a competitive inhibition method for CRP were employed in the LFS. For PCT and CRP in serum assayed by the dual-QDs-labeled LFS, their detection sensitivities reached 0.1 and 1 ng/mL, respectively, and their linear quantitative detection ranges were from 0.3 to 200 ng/mL and from 50 to 250 μg/mL, respectively. There was little evidence that the PCT and CRP assays would be interfered with each other. The correlations for testing CRP and PCT in clinical samples were 99.75 and 97.02 %, respectively, between the dual-QDs-labeled LFS we developed and commercial methods. The rapid quantification of PCT and CRP on dual-QDs-labeled LFS is of great clinical value to distinguish inflammation, bacterial infection, or viral infection and to provide guidance for the use of antibiotics or other medicines.
authors.affiliation: Shenzhen Sixth People's Hospital, Shenzhen, 518052, People's Republic of China.
authors.forename: XiaoPing
authors.initials: X
authors.lastname: Qi
authors.affiliation: Biomedical Engineering, Shenzhen Polytechnic, Shenzhen, 518055, People's Republic of China.
authors.forename: YunYe
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Shenzhen Institute for Drug Control, Shenzhen, 518057, People's Republic of China.
authors.forename: ZhongShi
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Shenzhen Institute for Drug Control, Shenzhen, 518057, People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Xu
authors.affiliation: Biomedical Engineering, Shenzhen Polytechnic, Shenzhen, 518055, People's Republic of China. yuhao_zju@163.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Yu
keywords: Blood; C-reactive protein; Lateral flow strip; Procalcitonin; Quantum dots