title: H19 Functions as a ceRNA in Promoting Metastasis Through Decreasing miR-200s Activity in Osteosarcoma.
abstract: Osteosarcoma is the most common primary bone sarcoma around the world. The poor prognosis and high recurrence rate of osteosarcoma are largely due to the high rate of pulmonary metastasis. H19 has been reported to play a potential role in osteosarcoma progression. However, the exact molecular mechanism of metastasis involving H19 remains unclear. In the present study, we performed gain- and loss-of-function assays and found that H19 promotes migration and invasion in osteosarcoma cells. Furthermore, we showed that H19 promotes metastasis through upregulation of ZEB1 and ZEB2 by competitively binding the miR-200 family. Thus, our findings suggest important roles of H19 in osteosarcoma metastasis and indicate its potential application in osteosarcoma therapy.
authors.affiliation: Department of Orthopedics, Cangzhou Central Hospital , Hebei, China .
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Cangzhou Central Hospital , Hebei, China .
authors.forename: Hanwen
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, Cangzhou Central Hospital , Hebei, China .
authors.forename: Yuejiang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, Cangzhou Central Hospital , Hebei, China .
authors.forename: Shuming
authors.initials: S
authors.lastname: Gao
authors.affiliation: Department of Orthopedics, Cangzhou Central Hospital , Hebei, China .
authors.forename: Cai
authors.initials: C
authors.lastname: Cheng
mesh_terms: D001859:Bone Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D012516:Osteosarcoma / Q000235:genetics* / Q000556:secondary; D062085:RNA, Long Noncoding / Q000235:genetics*; D015854:Up-Regulation
chemical_list: C094335:H19 long non-coding RNA; D035683:MicroRNAs; D062085:RNA, Long Noncoding