title: Synthesis, characterization of α-amino acid Schiff base derived Ru/Pt complexes: Induces cytotoxicity in HepG2 cell via protein binding and ROS generation.
abstract: We have synthesized two new complexes of platinum (1) and ruthenium (2) with α-amino acid, l-alanine, and 2,3-dihydroxybenzaldehyde derived Schiff base (L). The ligand and both complexes were characterized by using elemental analysis and several other spectroscopic techniques viz; IR, (1)H, (13)C NMR, EPR, and ESI-MS. Furthermore, the protein-binding ability of synthesized complexes was monitored by UV-visible, fluorescence and circular dichroism techniques with a model protein, human serum albumin (HSA). Both the PtL2 and RuL2 complexes displayed significant binding towards HSA. Also, in vitro cytotoxicity assay for both complexes was carried out on human hepatocellular carcinoma cancer (HepG2) cell line. The results showed concentration-dependent inhibition of cell viability. Moreover, the generation of reactive oxygen species was also evaluated, and results exhibited substantial role in cytotoxicity.
authors.affiliation: Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
authors.forename: Ali
authors.initials: A
authors.lastname: Alsalme
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering and Technology, Aligarh Muslim University, Aligarh 202002, India.
authors.forename: Sameen
authors.initials: S
authors.lastname: Laeeq
authors.affiliation: Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh 202002, India.
authors.forename: Sourabh
authors.initials: S
authors.lastname: Dwivedi
authors.affiliation: Department of Biochemistry, College of Science, King Saud University, Saudi Arabia.
authors.forename: Mohd Shahnawaz
authors.initials: MS
authors.lastname: Khan
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering and Technology, Aligarh Muslim University, Aligarh 202002, India.
authors.forename: Khalid
authors.initials: K
authors.lastname: Al Farhan
authors.affiliation: Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh 202002, India.
authors.forename: Javed
authors.initials: J
authors.lastname: Musarrat
authors.affiliation: Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia. Electronic address: raischem@gmail.com.
authors.forename: Rais Ahmad
authors.initials: RA
authors.lastname: Khan
mesh_terms: D000596:Amino Acids / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002942:Circular Dichroism; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000494:pharmacology*; D056945:Hep G2 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D007700:Kinetics; D008247:Lysosomes / Q000378:metabolism; D009499:Neutral Red / Q000378:metabolism; D010984:Platinum / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects; D017433:Protein Structure, Secondary; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012428:Ruthenium / Q000494:pharmacology*; D012545:Schiff Bases / Q000138:chemical synthesis / Q000494:pharmacology*; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence
chemical_list: D000596:Amino Acids; D056831:Coordination Complexes; D017382:Reactive Oxygen Species; D012545:Schiff Bases; D012709:Serum Albumin; D009499:Neutral Red; D010984:Platinum; D012428:Ruthenium
keywords: HSA binding; MTT assay; Metal complexes; ROS generation