title: [URINE UNDER CHRONIC DYSFUNCTION OF RENAL ALLO-TRANSPLANT].
abstract: The study was organized to provide additional characteristic of chronic dysfunction of renal allo-transplant using such biomarkers of serum and urine as enzymes (alanine aminotransferase), aspartate aminotransferase, gamma- glutamiltransferase, alkaline phosphatase, N-acetyl-β-D-glucosaminidase, interleukins (IL-2, IL-8, IL-10), beta-2- microglobulin. The chronic dysfunction of renal allo-transplant is characterized by increasing of concentration of IL-10 and beta-2-microglobulin in serum and increasing of concentration of beta-2-microglobulin, IL-2, IL-8 in urine and increasing of activity of N-acetyl-β-D-glucosaminidase, alkaline phosphatase, aspartate aminotransferase, gamma-glutamiltransferase as compared with patients with satisfactory function of renal allo-transplant. The multivariant logistic regression analysis established that only activity of N-acetyl-β-D-glucosaminidase in urine was reliably independently related to chronic dysfunction of renal allo-transplant. It is assumed that increasing of concentration of beta-2-microglobulin in serum testifies glomerular dysfunction and in urine--tubular dysfunction of renal allo-transplant. The enzymeuria indicates continuing damage of epithelium of proximal tubules of nephron. The classification of patients with satisfactory function and chronic dysfunction of renal allo-transplant established that the highest indicators of square under ROC-curves had concentration of beta-2-microglobulin in serum (0.858 ± 0.061) and urine (0.733 ± 0.079) and activity of N-acetyl-β-D-glucosaminidase in urine (0.701 ± 0.061). To specify diagnosis of chronic dysfunction of renal allo-transplant the most useful (ratio of likelihood of positive result 10 and 11 correspondingly) are tests of beta-2- microglobulin in serum (more than 8.55 mkg/ml) and N-acetyl-β-D-glucosaminidase/creatinine in urine (more than 34 nmol/(sl)/ mmol/l). These discoveries require further validation and confirmation by implementation of morphological analysis of bioptat of renal allo-transplant.
authors.forename: A V
authors.initials: AV
authors.lastname: Trailin
authors.forename: M V
authors.initials: MV
authors.lastname: Pleten
authors.forename: A S
authors.initials: AS
authors.lastname: Nikonenko
authors.forename: N E
authors.initials: NE
authors.lastname: Efimenko
authors.forename: T I
authors.initials: TI
authors.lastname: Ostapenko
mesh_terms: D000118:Acetylglucosaminidase / Q000097:blood / Q000652:urine*; D000293:Adolescent; D000328:Adult; D000410:Alanine Transaminase / Q000097:blood / Q000652:urine; D000469:Alkaline Phosphatase / Q000097:blood / Q000652:urine; D001219:Aspartate Aminotransferases / Q000097:blood / Q000652:urine; D015415:Biomarkers / Q000097:blood / Q000652:urine; D006801:Humans; D016753:Interleukin-10 / Q000097:blood* / Q000652:urine; D007376:Interleukin-2 / Q000097:blood / Q000652:urine*; D016209:Interleukin-8 / Q000097:blood / Q000652:urine*; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D012372:ROC Curve; D051436:Renal Insufficiency, Chronic / Q000097:blood / Q000175:diagnosis* / Q000473:pathology / Q000652:urine; D012189:Retrospective Studies; D014184:Transplantation, Homologous; D001613:beta 2-Microglobulin / Q000097:blood / Q000652:urine; D005723:gamma-Glutamyltransferase / Q000097:blood / Q000652:urine
chemical_list: D015415:Biomarkers; C508609:IL10 protein, human; D007376:Interleukin-2; D016209:Interleukin-8; D001613:beta 2-Microglobulin; D016753:Interleukin-10; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D000118:Acetylglucosaminidase