title: Ca2+/H+ exchange by acidic organelles regulates cell migration in vivo.
abstract: Increasing evidence implicates Ca(2+) in the control of cell migration. However, the underlying mechanisms are incompletely understood. Acidic Ca(2+) stores are fast emerging as signaling centers. But how Ca(2+) is taken up by these organelles in metazoans and the physiological relevance for migration is unclear. Here, we identify a vertebrate Ca(2+)/H(+)exchanger (CAX) as part of a widespread family of homologues in animals. CAX is expressed in neural crest cells and required for their migration in vivo. It localizes to acidic organelles, tempers evoked Ca(2+) signals, and regulates cell-matrix adhesion during migration. Our data provide new molecular insight into how Ca(2+) is handled by acidic organelles and link this to migration, thereby underscoring the role of noncanonical Ca(2+) stores in the control of Ca(2+)-dependent function.
authors.affiliation: Department of Cell and Developmental Biology, University College London, London WC1E 6BT, England, UK.
authors.forename: Manuela
authors.initials: M
authors.lastname: Melchionda
authors.affiliation: Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, England, UK.
authors.forename: Jon K
authors.initials: JK
authors.lastname: Pittman
authors.affiliation: Department of Cell and Developmental Biology, University College London, London WC1E 6BT, England, UK r.mayor@ucl.ac.uk patel.s@ucl.ac.uk.
authors.forename: Roberto
authors.initials: R
authors.lastname: Mayor
authors.affiliation: Department of Cell and Developmental Biology, University College London, London WC1E 6BT, England, UK r.mayor@ucl.ac.uk patel.s@ucl.ac.uk.
authors.forename: Sandip
authors.initials: S
authors.lastname: Patel
mesh_terms: D000818:Animals; D017920:Antiporters / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D002448:Cell Adhesion; D002465:Cell Movement; D030562:Databases, Protein; D005838:Genotype; D006367:HeLa Cells; D006801:Humans; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D009432:Neural Crest / Q000378:metabolism*; D015388:Organelles / Q000378:metabolism*; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D047270:Strongylocentrotus purpuratus / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D014162:Transfection; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis / Q000235:genetics / Q000378:metabolism
chemical_list: D017920:Antiporters; D029867:Xenopus Proteins; D006859:Hydrogen; D002118:Calcium