title: One-pot synthesis of a gold nanoparticle-Vmh2 hydrophobin nanobiocomplex for glucose monitoring.
abstract: HydrophobinVmh2 is a small amphiphilic protein, which self-assembles on different surfaces and naturally interacts with glucose. Here, we report on the synthesis of a nanobiocomplex made of polyethylene glycol, Vmh2 and gold nanoparticles by a one-step process and on its ability to recognise glucose in an aqueous solution at 0.3-0.6-1.2 mg ml(-1) concentrations. Even though the Vmh2 proteins are intrinsically bonded to the gold core, effective glucose interaction monitoring was demonstrated by using dynamic light scattering, ultraviolet-visible, polarization-modulated infrared reflection-absorption and x-ray photoelectron spectroscopies. Experimental results highlighted an affinity constant of 7.3 ± 0.3 mg ml(-1) between the nanobiosystem and the sugar, and a detection sensitivity of 0.13 ± 0.06 a.u./mg ml(-1).
authors.affiliation: Sorbonne Universités, UPMC Paris 06, Laboratoire de Réactivité de Surface, 4 place Jussieu, F-75005 Paris, France.|Institute for Microelectronics and Microsystems, Unit of Naples-National Research Council, Via P. Castellino 111, 80127, Italy.|Department of Chemical Sciences, University of Naples «Federico II», Via Cinthia, 80126 Naples, Italy.
authors.forename: Jane
authors.initials: J
authors.lastname: Politi
authors.affiliation: Institute for Microelectronics and Microsystems, Unit of Naples-National Research Council, Via P. Castellino 111, 80127, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: De Stefano
authors.affiliation: Institute for Microelectronics and Microsystems, Unit of Naples-National Research Council, Via P. Castellino 111, 80127, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Rea
authors.affiliation: Department of Chemical Sciences, University of Naples «Federico II», Via Cinthia, 80126 Naples, Italy.
authors.forename: Alfredo Maria
authors.initials: AM
authors.lastname: Gravagnuolo
authors.affiliation: Department of Chemical Sciences, University of Naples «Federico II», Via Cinthia, 80126 Naples, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Giardina
authors.affiliation: Sorbonne Universités, UPMC Paris 06, Laboratoire de Réactivité de Surface, 4 place Jussieu, F-75005 Paris, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Methivier
authors.affiliation: Sorbonne Universités, UPMC Paris 06, Laboratoire de Réactivité de Surface, 4 place Jussieu, F-75005 Paris, France.|CNRS, UMR 7244, CSPBAT, Laboratoire de Chimie, Structures et Propriétés de Biomateriaux et d'Agents Therapeutiques Université Paris 13, Sorbonne Paris Cité, Bobigny, France CNRS, Paris, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Casale
authors.affiliation: Sorbonne Universités, UPMC Paris 06, Laboratoire de Réactivité de Surface, 4 place Jussieu, F-75005 Paris, France.|CNRS, UMR 7244, CSPBAT, Laboratoire de Chimie, Structures et Propriétés de Biomateriaux et d'Agents Therapeutiques Université Paris 13, Sorbonne Paris Cité, Bobigny, France CNRS, Paris, France.
authors.forename: Jolanda
authors.initials: J
authors.lastname: Spadavecchia
mesh_terms: D005656:Fungal Proteins / Q000737:chemistry*; D005947:Glucose; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry; D013057:Spectrum Analysis
chemical_list: D005656:Fungal Proteins; D011092:Polyethylene Glycols; D006046:Gold; D005947:Glucose