title: Plant and soil fungal but not soil bacterial communities are linked in long-term fertilized grassland.
abstract: Inorganic fertilization and mowing alter soil factors with subsequent effects-direct and indirect - on above- and below-ground communities. We explored direct and indirect effects of long-term fertilization (N, P, NPK, Liming) and twice yearly mowing on the plant, bacterial and fungal communities and soil factors. We analyzed co-variation using 16S and 18S rRNA genes surveys, and plant frequency and edaphic factors across treatments. The plant and fungal communities were distinct in the NPK and L treatments, while the bacterial communities and soil factors were distinct in the N and L treatments. Plant community diversity and evenness had low diversity in the NPK and high diversity in the liming treatment, while the diversity and evenness of the bacterial and fungal communities did not differ across treatments, except of higher diversity and evenness in the liming treatment for the bacteria. We found significant co-structures between communities based on plant and fungal comparisons but not between plant and bacterial nor bacterial and fungal comparisons. Our results suggested that the plant and fungal communities are more tightly linked than either community with the bacterial community in fertilized soils. We found co-varying plant, bacterial and fungal taxa in different treatments that may indicate ecological interactions.
authors.affiliation: Netherlands Institute of Ecology NIOO-KNAW, Department of Microbial Ecology, Wageningen, 6708 PB, Netherlands.|Leiden University, Department of Biology, Leiden, 2311 EZ, Netherlands.
authors.forename: Noriko A
authors.initials: NA
authors.lastname: Cassman
authors.affiliation: Netherlands Institute of Ecology NIOO-KNAW, Department of Microbial Ecology, Wageningen, 6708 PB, Netherlands.|Maranhão State University, Department of Agroecology, São Luís Maranhão, 65055-000, Brazil.
authors.forename: Marcio F A
authors.initials: MF
authors.lastname: Leite
authors.affiliation: Netherlands Institute of Ecology NIOO-KNAW, Department of Microbial Ecology, Wageningen, 6708 PB, Netherlands.
authors.forename: Yao
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Netherlands Institute of Ecology NIOO-KNAW, Department of Microbial Ecology, Wageningen, 6708 PB, Netherlands.
authors.forename: Mattias
authors.initials: M
authors.lastname: de Hollander
authors.affiliation: Netherlands Institute of Ecology NIOO-KNAW, Department of Microbial Ecology, Wageningen, 6708 PB, Netherlands.|Leiden University, Department of Biology, Leiden, 2311 EZ, Netherlands.
authors.forename: Johannes A
authors.initials: JA
authors.lastname: van Veen
authors.affiliation: Netherlands Institute of Ecology NIOO-KNAW, Department of Microbial Ecology, Wageningen, 6708 PB, Netherlands.
authors.forename: Eiko E
authors.initials: EE
authors.lastname: Kuramae
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D005308:Fertilizers; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000502:physiology*; D065948:Grassland; D054884:Host-Pathogen Interactions; D064307:Microbiota / Q000235:genetics / Q000502:physiology*; D010944:Plants / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D013045:Species Specificity; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial; D004271:DNA, Fungal; D005308:Fertilizers; D012336:RNA, Ribosomal, 16S; D012337:RNA, Ribosomal, 18S; D012987:Soil