title: Human cathelicidin LL-37 inhibits platelet aggregation and thrombosis via Src/PI3K/Akt signaling.
abstract: Biological functions of human cathelicidin LL-37 have been widely reported, including antibacterial, immune and anti-tumor effects. However, the antiplatelet activity of LL-37 has not been addressed. The purpose of our study was to investigate the antiplatelet and antithrombotic actions of LL-37. We found that this peptide inhibited human platelet aggregation in vitro and attenuated thrombus formation in vivo. Furthermore, LL-37 reduced phosphorylation of Src kinase and Akt(Ser473), decreased platelet spreading on immobilized fibrinogen and inhibited P-selectin expression on platelets. These results demonstrate that LL-37 has antiplatelet and antithrombotic actions.
authors.affiliation: School of Life Science & Technology, China Pharmaceutical University, 24 Tong Jia Street, Nanjing 210009, People's Republic of China.
authors.forename: Wen
authors.initials: W
authors.lastname: Su
authors.affiliation: School of Life Science & Technology, China Pharmaceutical University, 24 Tong Jia Street, Nanjing 210009, People's Republic of China.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Life Science & Technology, China Pharmaceutical University, 24 Tong Jia Street, Nanjing 210009, People's Republic of China.
authors.forename: Caihui
authors.initials: C
authors.lastname: Wang
authors.affiliation: School of Life Science & Technology, China Pharmaceutical University, 24 Tong Jia Street, Nanjing 210009, People's Republic of China.
authors.forename: Xue
authors.initials: X
authors.lastname: Ding
authors.affiliation: School of Life Science & Technology, China Pharmaceutical University, 24 Tong Jia Street, Nanjing 210009, People's Republic of China.
authors.forename: Gamariel
authors.initials: G
authors.lastname: Rwibasira
authors.affiliation: School of Life Science & Technology, China Pharmaceutical University, 24 Tong Jia Street, Nanjing 210009, People's Republic of China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, 24 Tong Jia Street, Nanjing 210009, People's Republic of China. Electronic address: yikong668@163.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Kong
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry*; D001792:Blood Platelets / Q000378:metabolism; D005260:Female; D005340:Fibrinogen / Q000737:chemistry; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D019007:P-Selectin / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D010766:Phosphorylation; D015539:Platelet Activation / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D013927:Thrombosis / Q000188:drug therapy*; D019061:src-Family Kinases / Q000378:metabolism*; D054804:Cathelicidins
chemical_list: D023181:Antimicrobial Cationic Peptides; D005340:Fibrinogen; D019007:P-Selectin; D019869:Phosphatidylinositol 3-Kinases; D010975:Platelet Aggregation Inhibitors; D051057:Proto-Oncogene Proteins c-akt; D019061:src-Family Kinases; D054804:Cathelicidins; C494918:AKT1 protein, human; C494920:Akt1 protein, rat
keywords: Antiplatelet; Antithrombosis; LL-37; Src/PI3K/Akt signaling