title: Plastid ribosomal protein S5 is involved in photosynthesis, plant development, and cold stress tolerance in Arabidopsis.
abstract: Plastid ribosomal proteins are essential components of protein synthesis machinery and have diverse roles in plant growth and development. Mutations in plastid ribosomal proteins lead to a range of developmental phenotypes in plants. However, how they regulate these processes is not fully understood, and the functions of some individual plastid ribosomal proteins remain unknown. To identify genes responsible for chloroplast development, we isolated and characterized a mutant that exhibited pale yellow inner leaves with a reduced growth rate in Arabidopsis. The mutant (rps5) contained a missense mutation of plastid ribosomal protein S5 (RPS5), which caused a dramatically reduced abundance of chloroplast 16S rRNA and seriously impaired 16S rRNA processing to affect ribosome function and plastid translation. Comparative proteomic analysis revealed that the rps5 mutation suppressed the expression of a large number of core components involved in photosystems I and II as well as many plastid ribosomal proteins. Unexpectedly, a number of proteins associated with cold stress responses were greatly decreased in rps5, and overexpression of the plastid RPS5 improved plant cold stress tolerance. Our results indicate that RPS5 is an important constituent of the plastid 30S subunit and affects proteins involved in photosynthesis and cold stress responses to mediate plant growth and development.
authors.affiliation: College of Horticulture, State Key Laboratory of Crop Stress Biology for Arid Area, Northwest A&F University, Yangling, 712100, China Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Junxiang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Hui
authors.initials: H
authors.lastname: Yuan
authors.affiliation: Robert W. Holley Center for Agriculture and Health, USDA-ARS, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Robert W. Holley Center for Agriculture and Health, USDA-ARS, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Tara
authors.initials: T
authors.lastname: Fish
authors.affiliation: Robert W. Holley Center for Agriculture and Health, USDA-ARS, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Sangbom M
authors.initials: SM
authors.lastname: Lyi
authors.affiliation: Robert W. Holley Center for Agriculture and Health, USDA-ARS, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Theodore W
authors.initials: TW
authors.lastname: Thannhauser
authors.affiliation: College of Horticulture, State Key Laboratory of Crop Stress Biology for Arid Area, Northwest A&F University, Yangling, 712100, China.
authors.forename: Lugang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University, Ithaca, NY 14853, USA Robert W. Holley Center for Agriculture and Health, USDA-ARS, Cornell University, Ithaca, NY 14853, USA ll37@cornell.edu.
authors.forename: Li
authors.initials: L
authors.lastname: Li
mesh_terms: D017360:Arabidopsis / Q000254:growth & development / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology*; D003080:Cold Temperature; D020125:Mutation, Missense; D010788:Photosynthesis / Q000502:physiology; D018087:Plastids / Q000502:physiology; D012336:RNA, Ribosomal, 16S / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013312:Stress, Physiological / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; D012336:RNA, Ribosomal, 16S; C465630:RPS5 protein, Arabidopsis
keywords: Arabidopsis; RPS5.; cold stress tolerance; photosynthesis; plastid ribosomal protein; proteomics