title: HLA-B allele dropout in PCR sequence-specific oligonucleotide probe typing due to intronic polymorphism in the novel B*58:01:01:02 allele.
abstract: Currently, Luminex technology based on the PCR sequence-specific oligonucleotide (SSO) probe method has been widely used for HLA genotyping in the immunogenetics laboratories. Here, we reported a case with HLA-B allele dropout by Luminex technology. The initial HLA-B result of the Luminex method with a commercial agent kit was inconclusive, and then, the result of PCR-SBT technology indicated the dropout as a HLA-B*58 allele. Subsequently, the full-length sequence of HLA-B allele was determined by TOPO-TA cloning, and a novel allele B*58:01:01:02 was identified in the individual. Compared with HLA-B*58:01:01:01, the novel allele showed some nucleotides difference at 509 C>T, 521 T>G and CCC insertion in position 503 of intron 2. According to the full-length sequence, the new mutations of intron 2 were contributed to HLA-B locus allele dropout in the sample. Our results indicated multiplatform should be used to improve the HLA typing accuracy when a conclusive HLA genotype cannot be determined.
authors.affiliation: Blood Center of Zhejiang Province, Hangzhou, China.|Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, China.|Zhejiang Provincial Key Laboratory of Blood Safety Research, Hangzhou, Zhejiang, China.
authors.forename: Y
authors.initials: Y
authors.lastname: He
authors.affiliation: Blood Center of Zhejiang Province, Hangzhou, China.|Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, China.
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.affiliation: Blood Center of Zhejiang Province, Hangzhou, China.|Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Han
authors.affiliation: Blood Center of Zhejiang Province, Hangzhou, China.|Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, China.|Zhejiang Provincial Key Laboratory of Blood Safety Research, Hangzhou, Zhejiang, China.
authors.forename: J
authors.initials: J
authors.lastname: He
authors.affiliation: Blood Center of Zhejiang Province, Hangzhou, China.|Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, China.
authors.forename: N
authors.initials: N
authors.lastname: Chen
authors.affiliation: Blood Center of Zhejiang Province, Hangzhou, China.|Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, China.
authors.forename: L
authors.initials: L
authors.lastname: Dong
authors.affiliation: Blood Center of Zhejiang Province, Hangzhou, China.|Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, China.|Zhejiang Provincial Key Laboratory of Blood Safety Research, Hangzhou, Zhejiang, China.
authors.forename: S
authors.initials: S
authors.lastname: Tao
authors.affiliation: Blood Center of Zhejiang Province, Hangzhou, China.|Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, China.|Zhejiang Provincial Key Laboratory of Blood Safety Research, Hangzhou, Zhejiang, China.
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Blood Center of Zhejiang Province, Hangzhou, China.|Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, China.|Zhejiang Provincial Key Laboratory of Blood Safety Research, Hangzhou, Zhejiang, China.
authors.forename: J
authors.initials: J
authors.lastname: He
authors.affiliation: Blood Center of Zhejiang Province, Hangzhou, China.|Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, China.|Zhejiang Provincial Key Laboratory of Blood Safety Research, Hangzhou, Zhejiang, China.
authors.forename: F
authors.initials: F
authors.lastname: Zhu
authors.affiliation: Blood Center of Zhejiang Province, Hangzhou, China.|Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, China.|Zhejiang Provincial Key Laboratory of Blood Safety Research, Hangzhou, Zhejiang, China.
authors.forename: H
authors.initials: H
authors.lastname: Lv
mesh_terms: D000483:Alleles; D005838:Genotype; D015235:HLA-B Antigens / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D007438:Introns / Q000235:genetics; D015345:Oligonucleotide Probes / Q000235:genetics
chemical_list: D015235:HLA-B Antigens; D015345:Oligonucleotide Probes