title: [The Comparison of Real-Time PCR and direct immunofluorescence in laboratory diagnostics of chlamydiosis in patients of Department of Dermatology and Venereology Medical University of Warsaw].
abstract: BACKGROUND
  Chlamydial infection is one of the most common bacterial sexually transmitted diseases. The aim of the study was to compare Real Time PCR and Direct Immunofluorescence (DIF) in laboratory diagnostics of Chlamydia trachomatis infection in patients of Department of Dermatology and Venereology in Warsaw.
  
  
  METHODS
  We investigated the urethral, cervical, anal and pharyngeal specimens from 152 patients of Department of Dermatology and Venereology in Warsaw. We used DNA Bact Extra Pure Kit (Euroclone®) to isolate DNA. Real Time PCR DUPLICα® 2nd Generation Detection Real Time Advanced Dual Easy Chlamydia trachomatis Kit (Euroclone®) was performed on termocycler Smart Cycler® Dx. For direct immunofluorescence we used MicroTrack Chlamydia trachomatis Direct Specimen Test (Trinity Biotech).
  
  
  RESULTS
  In 90% of cases of Real Time PCR and DIF were consistent. 9% of samples were DIF negative and PCR positive and 1% were PCR negative and DIF positive. The results of PCR and DIF were identical more often in woman (98%)than in men (84%). In patients after antibiotics treatment the results of the two tests were consistent in 88%.
  
  
  CONCLUSIONS
  In laboratory diagnostics of C. trachomatis infections the results of the Real Time PCR and DIF were highly consistent although Real Time PCR. was more sensitive than DIF.
authors.forename: Ewa
authors.initials: E
authors.lastname: Skulska
authors.forename: Beata
authors.initials: B
authors.lastname: Młynarczyk-Bonikowska
authors.forename: Szymon
authors.initials: S
authors.lastname: Walter de Walthoffen
authors.forename: Grażyna
authors.initials: G
authors.lastname: Młynarczyk
authors.forename: Magdalena
authors.initials: M
authors.lastname: Malejczyk
authors.forename: Sławomir
authors.initials: S
authors.lastname: Majewski
mesh_terms: D001003:Anal Canal / Q000382:microbiology; D002584:Cervix Uteri / Q000382:microbiology; D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000302:isolation & purification; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D008297:Male; D010614:Pharynx / Q000382:microbiology; D060888:Real-Time Polymerase Chain Reaction; D014521:Urethra / Q000382:microbiology