title: The Role of Lumbar Sympathetic Nerves in Regulation of Blood Flow to Skeletal Muscle during Anaphylactic Hypotension in Anesthetized Rats.
abstract: During hypovolemic shock, skeletal muscle blood flow could be redistributed to vital organs via vasoconstriction in part evoked by activation of the innervating sympathetic nerve activity. However, it is not well known whether this mechanism operates during anaphylactic shock. We determined the femoral artery blood flow (FBF) and lumbar sympathetic nerve activity (LSNA) mainly regulating the hindquater muscle blood flow during anaphylactic hypotension in anesthetized rats. Anesthetized Sprague-Dawley rats were randomly allocated to the following groups (n = 7/group): (1) non-sensitized, (2) anaphylaxis, (3) anaphylaxis-lumbar sympathectomy (LS) and (4) anaphylaxis-sinoaortic denervation (SAD) groups. Anaphylaxis was induced by an intravenous injection of the ovalbumin antigen to the sensitized rats. The systemic arterial pressure (SAP), heart rate (HR), central venous pressure (CVP), FBF and LSNA were continuously measured. In the anaphylaxis group, LSNA and HR increased, while SAP and FBF decreased after antigen injection. In the anaphylaxis-SAD group, LSNA did not significantly change during the early phase, but the responses of SAP and FBF were similar to those in the anaphylaxis group. In the anaphylaxis-LS group, both FBF and SAP decreased similarly to the anaphylaxis group during anaphylactic hypotension. These results indicated that LSNA increased via baroreceptor reflex, but this sympathoexcitation or LS did not affect antigen-induced decreases in FBF or SAP. Lumbar sympathetic nerves are not involved in regulation of the blood flow to the hindlimb or systemic blood pressure during anaphylactic hypotension in anesthetized rats.
authors.affiliation: Department of Physiology II, Kanazawa Medical University, Uchinada, 920-0293, Japan.|Department of Anesthesiology, China-Japan Friendship Hospital, Beijing, 100029, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Song
authors.affiliation: Department of Physiology II, Kanazawa Medical University, Uchinada, 920-0293, Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Tanida
authors.affiliation: Department of Physiology II, Kanazawa Medical University, Uchinada, 920-0293, Japan.
authors.forename: Toshishige
authors.initials: T
authors.lastname: Shibamoto
authors.affiliation: Department of Physiology II, Kanazawa Medical University, Uchinada, 920-0293, Japan.|Department of Colorectal and Hernia Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang, 110032, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Physiology II, Kanazawa Medical University, Uchinada, 920-0293, Japan.|Department of Diabetes Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang, 110032, China.
authors.forename: Mofei
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Physiology II, Kanazawa Medical University, Uchinada, 920-0293, Japan.
authors.forename: Yuhichi
authors.initials: Y
authors.lastname: Kuda
authors.affiliation: Department of Physiology II, Kanazawa Medical University, Uchinada, 920-0293, Japan.
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Kurata
mesh_terms: D000707:Anaphylaxis / Q000150:complications* / Q000503:physiopathology*; D000758:Anesthesia; D000818:Animals; D003714:Denervation; D005263:Femoral Artery / Q000503:physiopathology; D006439:Hemodynamics; D007022:Hypotension / Q000150:complications* / Q000503:physiopathology*; D008159:Lumbar Vertebrae / Q000503:physiopathology*; D008297:Male; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000503:physiopathology; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow; D013562:Sympathectomy; D013564:Sympathetic Nervous System / Q000503:physiopathology*; D014655:Vascular Resistance