title: Effect of calcium on cell-wall degrading enzymes of Botrytis cinerea.
abstract: Effective anti-Botrytis strategies leading to reduce pesticides on strawberries are examined to provide the protection that is harmless to humans, higher animals and plants. Calcium treatments significantly inhibited the spore germination and mycelial growth of B. cinerea. The intracellular polygalacturonase and CMCase showed low activities in B. cinerea cultivated by medium containing calcium. On the other hand, calcium-stimulated β-glucosidases production occurred. Our findings suggest that the calcium treatments keep CMCase activity low and cause low activities of cell-wall degrading enzymes of B. cinerea in the late stage of growth.
authors.affiliation: a Department of Materials Chemistry and Bioengineering, National Institute of Technology , Oyama College , Oyama , Japan.
authors.forename: Izumi
authors.initials: I
authors.lastname: Sasanuma
authors.affiliation: a Department of Materials Chemistry and Bioengineering, National Institute of Technology , Oyama College , Oyama , Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Suzuki
mesh_terms: D020171:Botrytis / Q000737:chemistry / Q000201:enzymology*; D002118:Calcium / Q000494:pharmacology*; D002473:Cell Wall / Q000187:drug effects; D002480:Cellulase / Q000096:biosynthesis / Q000737:chemistry; D031985:Fragaria / Q000254:growth & development / Q000382:microbiology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D010575:Pesticides / Q000633:toxicity; D011096:Polygalacturonase / Q000096:biosynthesis / Q000737:chemistry; D013172:Spores, Fungal / Q000187:drug effects* / Q000472:pathogenicity
chemical_list: D010575:Pesticides; D011096:Polygalacturonase; D002480:Cellulase; C019826:carboxymethylcellulase; D002118:Calcium
keywords: Botrytis cinerea; calcium; cell-wall degrading enzyme; phytopathogen; strawberry