title: Relationship between Virucidal Efficacy and Free Iodine Concentration of Povidone-Iodine in Buffer Solution.
abstract: Povidone-iodine solutions prepared to various concentrations (0.01, 0.1, 1 and 10%) with 0.2M phosphate buffer (pH 7.0) (PVP-I PB) were analyzed to determine their free iodine concentrations using membrane permeation cells, and their inactivation effects on three viruses (influenza A virus, poliovirus type 1 and adenovirus type 3) were examined. The free iodine concentrations in the 0.01-10% PVP-I PB were determined to be 1.84, 4.88, 1.58 and 0.17 ppm (approximate values), respectively, with the maximum obtained for the 0.1% solution. The virucidal efficacy of these PVP-I PB against poliovirus type 1 and adenovirus type 3 was found to be generally dependent on free iodine concentration, with the 0.1% solution being the most effective. Influenza A virus was inactivated with an action time of 15 s at all four concentrations examined. The results of this study suggested an association between free iodine concentration and virucidal efficacy for the 0.01-10% PVP-I PB.
authors.affiliation: Yoshida Pharmaceutical CO., LTD. Quality Control Dept.
authors.forename: Hideki
authors.initials: H
authors.lastname: Wada
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Nojima
authors.forename: Satoko
authors.initials: S
authors.lastname: Ogawa
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Hayashi
authors.forename: Noriyoshi
authors.initials: N
authors.lastname: Sugiyama
authors.forename: Takumi
authors.initials: T
authors.lastname: Kajiura
authors.forename: Tomofumi
authors.initials: T
authors.lastname: Ueda
authors.forename: Shota
authors.initials: S
authors.lastname: Morimoto
authors.forename: Katuhiro
authors.initials: K
authors.lastname: Yokota
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D002021:Buffers; D007455:Iodine / Q000737:chemistry / Q000494:pharmacology*; D050296:Microbial Viability / Q000187:drug effects; D011206:Povidone-Iodine / Q000737:chemistry / Q000494:pharmacology*; D014780:Viruses / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D002021:Buffers; D011206:Povidone-Iodine; D007455:Iodine