title: A quinone mediator drives oxidations catalysed by alcohol dehydrogenase-containing cell lysates.
abstract: Spontaneous electron transport to molecular oxygen led to regeneration of oxidised nicotinamide cofactor in cell lysates that contain an alcohol dehydrogenase, a quinone reductase and a quinone mediator. This concept allows the efficient oxidation of alcohols in the presence of alcohol dehydrogenase-containing E. coli lysates and catalytic amounts of the quinone lawsone.
authors.affiliation: Institute of Pharmaceutical Sciences, University of Freiburg, Albertstraße 25, 79104 Freiburg, Germany. steffen.luedeke@pharmazie.uni-freiburg.de.
authors.forename: Julian
authors.initials: J
authors.lastname: Haas
authors.forename: Michael A
authors.initials: MA
authors.lastname: Schätzle
authors.forename: Syed M
authors.initials: SM
authors.lastname: Husain
authors.forename: Johannes
authors.initials: J
authors.lastname: Schulz-Fincke
authors.forename: Manfred
authors.initials: M
authors.lastname: Jung
authors.forename: Werner
authors.initials: W
authors.lastname: Hummel
authors.forename: Michael
authors.initials: M
authors.lastname: Müller
authors.forename: Steffen
authors.initials: S
authors.lastname: Lüdeke
mesh_terms: D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D000438:Alcohols / Q000378:metabolism*; D055162:Biocatalysis; D004579:Electron Transport; D004926:Escherichia coli / Q000201:enzymology*; D010084:Oxidation-Reduction; D011809:Quinones / Q000378:metabolism*
chemical_list: D000438:Alcohols; D011809:Quinones; D000426:Alcohol Dehydrogenase