title: Identification and Biological Activities of Long-Chain Peptaibols Produced by a Marine-Derived Strain of Trichoderma longibrachiatum.
abstract: Six long-chain peptaibols, 1 - 6, were identified from agar cultures of a marine-derived Trichoderma longibrachiatum Rifai strain (MMS151) isolated from blue mussels. The structure elucidation was carried out using electrospray ionization ion trap mass spectrometry (ESI-IT-MS) and GC/EI-MS. The long-chain peptaibols exhibited the general building scheme Ac-Aib-Ala-Aib-Ala-Aib-XXX-Gln-Aib-Vxx-Aib-Gly-XXX-Aib-Pro-Vxx-Aib-XXX-Gln-Gln-Pheol and were similar or identical to recurrent 20-residue peptaibols produced by Trichoderma spp. Three new sequences were identified and were called longibrachins A-0, A-II-a, and A-IV-b. The isolated peptaibols were assayed for cytotoxic, antibacterial, and antifungal activities, and acute toxicity on Dipteran larvae.
authors.affiliation: Département de Biotechnologie, Faculté des Sciences de la Nature et de la Vie, Université des Sciences et de la Technologie d'Oran-Mohamed Boudiaf (USTO-MB), El Mnaouar, B.P. 1505, Bir El Djir, 31000, Oran, Algeria. professionnelle@hotmail.ca.
authors.forename: Mustapha
authors.initials: M
authors.lastname: Mohamed-Benkada
authors.affiliation: Faculté de Pharmacie, MMS, Université de Nantes, FR-44000, Nantes.
authors.forename: Yves
authors.initials: Y
authors.lastname: François Pouchus
authors.affiliation: Laboratoire de Chimie Analytique, Faculté de Médecine et Pharmacie, Université de Rouen, FR-76000, Rouen.
authors.forename: Philippe
authors.initials: P
authors.lastname: Vérité
authors.affiliation: Laboratoire de Parasitologie et Mycologie Médicale, IICiMed, Faculté de Pharmacie, Université de Nantes, FR-44000, Nantes.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Pagniez
authors.affiliation: Laboratoire Thérapeutiques Cliniques et Expérimentales des Infections, Faculté de Médecine, Université de Nantes, FR-44000, Nantes.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Caroff
authors.affiliation: Faculté de Pharmacie, MMS, Université de Nantes, FR-44000, Nantes.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Ruiz
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D001688:Biological Products / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004175:Diptera / Q000187:drug effects / Q000196:embryology; D004354:Drug Screening Assays, Antitumor; D005658:Fungi / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D054848:Peptaibols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D014242:Trichoderma / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D000972:Antineoplastic Agents, Phytogenic; D001688:Biological Products; D054848:Peptaibols
keywords: Long-chain peptaibols; Longibrachins A-0, A-II-a, and A-IV-b; Marine-derived fungi; Trichoderma longibrachiatum