title: Detection of Recently Discovered Human Polyomaviruses in a Longitudinal Kidney Transplant Cohort.
abstract: A large number of human polyomaviruses have been discovered in the last 7 years. However, little is known about the clinical impact on vulnerable immunosuppressed patient populations. Blood, urine, and respiratory swabs collected from a prospective, longitudinal adult kidney transplant cohort (n = 167) generally pre-operatively, at day 4, months 1, 3, and 6 posttransplant, and at BK viremic episodes within the first year were screened for 12 human polyomaviruses using real-time polymerase chain reaction. Newly discovered polyomaviruses were most commonly detected in the respiratory tract, with persistent shedding seen for up to 6 months posttransplant. Merkel cell polyomavirus was the most common detection, but was not associated with clinical symptoms or subsequent development of skin cancer or other skin abnormalities. In contrast, KI polyomavirus was associated with respiratory disease in a subset of patients. Human polyomavirus 9, Malawi polyomavirus, and human polyomavirus 12 were not detected in any patient samples.
authors.affiliation: Centre for Children's Health Research, Children's Health Queensland, Brisbane, Australia.|Child Health Research Centre, The University of Queensland, Brisbane, Australia.
authors.forename: S
authors.identifier: 0000-0001-9474-6943
authors.initials: S
authors.lastname: Bialasiewicz
authors.affiliation: Child Health Research Centre, The University of Queensland, Brisbane, Australia.|Institute of Clinical Pathology and Medical Research, Westmead Hospital, Sydney, Australia.
authors.forename: R J
authors.initials: RJ
authors.lastname: Rockett
authors.affiliation: Department of Renal Medicine, Royal Melbourne Hospital, Melbourne, Australia.
authors.forename: K A
authors.initials: KA
authors.lastname: Barraclough
authors.affiliation: Department of Renal Medicine, Princess Alexandra Hospital, Brisbane, Australia.
authors.forename: D
authors.initials: D
authors.lastname: Leary
authors.affiliation: Institute for Future Environments, Central Analytical Research Facility, Queensland University of Technology, Brisbane, Australia.
authors.forename: K J
authors.initials: KJ
authors.lastname: Dudley
authors.affiliation: Department of Renal Medicine, Princess Alexandra Hospital, Brisbane, Australia.
authors.forename: N M
authors.initials: NM
authors.lastname: Isbel
authors.affiliation: Centre for Children's Health Research, Children's Health Queensland, Brisbane, Australia.|Child Health Research Centre, The University of Queensland, Brisbane, Australia.
authors.forename: T P
authors.initials: TP
authors.lastname: Sloots
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001315:Australia / Q000453:epidemiology; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005500:Follow-Up Studies; D005919:Glomerular Filtration Rate; D006084:Graft Rejection / Q000453:epidemiology / Q000821:virology*; D006085:Graft Survival; D006801:Humans; D016867:Immunocompromised Host; D007676:Kidney Failure, Chronic / Q000601:surgery*; D007677:Kidney Function Tests; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D011120:Polyomavirus / Q000235:genetics / Q000302:isolation & purification*; D027601:Polyomavirus Infections / Q000453:epidemiology / Q000821:virology*; D011379:Prognosis; D011446:Prospective Studies; D012137:Respiratory System / Q000821:virology*; D012307:Risk Factors; D014412:Tumor Virus Infections / Q000453:epidemiology / Q000821:virology*; D055815:Young Adult
chemical_list: D004279:DNA, Viral
keywords: basic (laboratory) research/science; complication: infectious; infection and infectious agents; infectious disease; kidney transplantation/nephrology; translational research/science; viral