title: Impact of Lipoprotein Lipase Gene Polymorphism, S447X, on Postprandial Triacylglycerol and Glucose Response to Sequential Meal Ingestion.
abstract: Lipoprotein lipase (LPL) is a key rate-limiting enzyme for the hydrolysis of triacylglycerol (TAG) in chylomicrons and very low-density lipoprotein. Given that postprandial assessment of lipoprotein metabolism may provide a more physiological perspective of disturbances in lipoprotein homeostasis compared to assessment in the fasting state, we have investigated the influence of two commonly studied LPL polymorphisms (rs320, HindIII; rs328, S447X) on postprandial lipaemia, in 261 participants using a standard sequential meal challenge. S447 homozygotes had lower fasting HDL-C (p = 0.015) and a trend for higher fasting TAG (p = 0.057) concentrations relative to the 447X allele carriers. In the postprandial state, there was an association of the S447X polymorphism with postprandial TAG and glucose, where S447 homozygotes had 12% higher TAG area under the curve (AUC) (p = 0.037), 8.4% higher glucose-AUC (p = 0.006) and 22% higher glucose-incremental area under the curve (IAUC) (p = 0.042). A significant gene-gender interaction was observed for fasting TAG (p = 0.004), TAG-AUC (Pinteraction = 0.004) and TAG-IAUC (Pinteraction = 0.016), where associations were only evident in men. In conclusion, our study provides novel findings of an effect of LPL S447X polymorphism on the postprandial glucose and gender-specific impact of the polymorphism on fasting and postprandial TAG concentrations in response to sequential meal challenge in healthy participants.
authors.affiliation: Hugh Sinclair Unit of Human Nutrition, Department of Food and Nutritional Sciences, University of Reading, Reading RG6 6AP, UK. i.m.a.shatwan@pgr.reading.ac.uk.|Food and Nutrition Department, Faculty of Home Economics, King Abdulaziz University, Jeddah 21589, Saudi Arabia. i.m.a.shatwan@pgr.reading.ac.uk.
authors.forename: Israa M
authors.initials: IM
authors.lastname: Shatwan
authors.affiliation: Hugh Sinclair Unit of Human Nutrition, Department of Food and Nutritional Sciences, University of Reading, Reading RG6 6AP, UK. A.Minihane@uea.ac.uk.|Department of Nutrition and Preventive Medicine, Norwich Medical School, University of East Anglia, Norwich NR4 7TJ, UK. A.Minihane@uea.ac.uk.
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Minihane
authors.affiliation: Institute for Cardiovascular and Metabolic Research (ICMR), University of Reading, Reading RG6 6AS, UK. c.m.williams@reading.ac.uk.
authors.forename: Christine M
authors.initials: CM
authors.lastname: Williams
authors.affiliation: Hugh Sinclair Unit of Human Nutrition, Department of Food and Nutritional Sciences, University of Reading, Reading RG6 6AP, UK. j.a.lovegrove@reading.ac.uk.|Institute for Cardiovascular and Metabolic Research (ICMR), University of Reading, Reading RG6 6AS, UK. j.a.lovegrove@reading.ac.uk.
authors.forename: Julie A
authors.initials: JA
authors.lastname: Lovegrove
authors.affiliation: Hugh Sinclair Unit of Human Nutrition, Department of Food and Nutritional Sciences, University of Reading, Reading RG6 6AP, UK. k.g.jackson@reading.ac.uk.|Institute for Cardiovascular and Metabolic Research (ICMR), University of Reading, Reading RG6 6AS, UK. k.g.jackson@reading.ac.uk.
authors.forename: Kim G
authors.initials: KG
authors.lastname: Jackson
authors.affiliation: Hugh Sinclair Unit of Human Nutrition, Department of Food and Nutritional Sciences, University of Reading, Reading RG6 6AP, UK. v.karani@reading.ac.uk.|Institute for Cardiovascular and Metabolic Research (ICMR), University of Reading, Reading RG6 6AS, UK. v.karani@reading.ac.uk.
authors.forename: Karani S
authors.initials: KS
authors.lastname: Vimaleswaran
mesh_terms: D000328:Adult; D000368:Aged; D001786:Blood Glucose / Q000378:metabolism*; D004063:Digestion; D005260:Female; D006720:Homozygote; D006801:Humans; D006949:Hyperlipidemias / Q000235:genetics*; D008071:Lipoprotein Lipase / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D019518:Postprandial Period; D012737:Sex Factors; D014280:Triglycerides / Q000097:blood*
chemical_list: D001786:Blood Glucose; D014280:Triglycerides; D008071:Lipoprotein Lipase
keywords: glucose; lipoprotein lipase; postprandial study; sequential meals; triacylglycerol