title: MicroRNA-371-5p targets SOX2 in gastric cancer.
abstract: We evaluated miR-371-5p expression in gastric cancer (GC) tissues and its influence on the expression of downstream genes, especially SOX2. MiR-371-5p expression (measured using qRT-PCR) was upregulated in GC tissues and correlated positively with TNM staging and lymph node (LN) metastasis. MiR-371-5p expression was higher in human GC cell lines (AGS, MKN-28, BGC-823, MGC-803, SGC-7901 and MKN-45) than in human normal gastric epithelial (GES-1) cells (all P < 0.05). MGC-803 tumor cell growth (measured with an MTT assay), migration, and invasion (measured with Transwell chamber assays) were severely inhibited in cells transfected with a miR-371-5p inhibitor, whereas they were stimulated in cells transfected with SOX2 siRNA or miR-371-5p inhibitor + SOX2 siRNA. Expression of SOX2 mRNA and protein (assessed with qRT-PCR and Western blot) were greatly enhanced in the miR-371-5p inhibitor group. These results indicate that miR-371-5p expression is strongly upregulated in GC tissues and negatively correlated with SOX2 expression, while miR-371-5p expression is inversely related to proliferation, TNM stage, and LN metastasis of GC cells. Suppression of miR-371-5p may inhibit the growth and invasion of MGC-803 GC cells by upregulating SOX2 expression.
authors.affiliation: Department of General Surgery, The First Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning, P.R. China.
authors.forename: Yu-Ji
authors.initials: YJ
authors.lastname: Li
authors.affiliation: Department of General Surgery, The First Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning, P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Dong
authors.affiliation: Department of General Surgery, The First Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning, P.R. China.
authors.forename: Fan-Min
authors.initials: FM
authors.lastname: Kong
authors.affiliation: Department of General Surgery, The First Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning, P.R. China.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Zhou
authors.affiliation: Department of General Surgery, The First Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning, P.R. China.
authors.forename: Dong
authors.initials: D
authors.lastname: Liang
authors.affiliation: Department of General Surgery, The First Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning, P.R. China.
authors.forename: Huan-Zhou
authors.initials: HZ
authors.lastname: Xue
mesh_terms: D000328:Adult; D000368:Aged; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D034622:RNA Interference; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors; D014162:Transfection
chemical_list: D019394:Ki-67 Antigen; C554834:MIRN371 microRNA, human; D035683:MicroRNAs; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors
keywords: SOX2; diological significance; gastric cancer; microRNA-371-5p