title: Differential Response of Two Human Breast Cancer Cell Lines to the Phenolic Extract from Flaxseed Oil.
abstract: Many studies have evidenced that the phenolic components from flaxseed (FS) oil have potential health benefits. The effect of the phenolic extract from FS oil has been evaluated on two human breast cancer cell lines, MCF7 and MDA-MB231, and on the human non-cancerous breast cell line, MCF10A, by SRB assay, cellular death, cell cycle, cell signaling, lipid peroxidation and expression of some key genes. We have evidenced that the extract shows anti-proliferative activity on MCF7 cells by inducing cellular apoptosis, increase of the percentage of cells in G0/G1 phase and of lipid peroxidation, activation of the H2AX signaling pathway, and upregulation of a six gene signature. On the other hand, on the MDA-MB2131 cells we verified only an anti-proliferative activity, a weak lipid peroxidation, the activation of the PI3K signaling pathway and an up-regulation of four genes. Overall these data suggest that the extract has both cytotoxic and pro-oxidant effects only on MCF7 cells, and can act as a metabolic probe, inducing differences in the gene expression. For this purpose, we have performed an interactomic analysis, highlighting the existing associations. From this approach, we show that the phenotypic difference between the two cell lines can be explained through their differential response to the phenolic extract.
authors.affiliation: CROM, Istituto Nazionale Tumori "Fondazione G. Pascale"-IRCCS, Napoli 80131, Italy. a.sorice@istitutotumori.na.it.
authors.forename: Angela
authors.initials: A
authors.lastname: Sorice
authors.affiliation: CROM, Istituto Nazionale Tumori "Fondazione G. Pascale"-IRCCS, Napoli 80131, Italy. e.guerriero@istitutotumori.na.it.
authors.forename: Eliana
authors.initials: E
authors.lastname: Guerriero
authors.affiliation: Istituto di Scienze dell'Alimentazione, CNR, Avellino 83100, Italy. mgvolpe@isa.cnr.it.
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Volpe
authors.affiliation: CROM, Istituto Nazionale Tumori "Fondazione G. Pascale"-IRCCS, Napoli 80131, Italy. f.capone@istitutotumori.na.it.
authors.forename: Francesca
authors.initials: F
authors.lastname: Capone
authors.affiliation: Istituto di Biologia Agro-ambientale e Forestale, CNR, UOS di Napoli, Napoli 80131, Italy. francesco.lacara@cnr.it.
authors.forename: Francesco
authors.initials: F
authors.lastname: La Cara
authors.affiliation: Direttore Scientifico, Istituto Nazionale Tumori "Fondazione G. Pascale"-IRCCS, Napoli 80131, Italy. g.ciliberto@istitutotumori.na.it.
authors.forename: Gennaro
authors.initials: G
authors.lastname: Ciliberto
authors.affiliation: Servizio di Informatica Medica, Azienda Ospedaliera Universitaria, Seconda Università di Napoli, Napoli 80138, Italy. giovanni.colonna@unina2.it.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Colonna
authors.affiliation: CROM, Istituto Nazionale Tumori "Fondazione G. Pascale"-IRCCS, Napoli 80131, Italy. s.costantini@istitutotumori.na.it.
authors.forename: Susan
authors.initials: S
authors.lastname: Costantini
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006657:Histones / Q000096:biosynthesis; D006801:Humans; D008043:Linseed Oil / Q000008:administration & dosage*; D015227:Lipid Peroxidation / Q000187:drug effects; D061986:MCF-7 Cells; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: C468783:H2AX protein, human; D006657:Histones; D017382:Reactive Oxygen Species; D008043:Linseed Oil
keywords: breast cancer cell lines; flaxseed; gene regulation; phenolic extract