title: Comparative Proteomic Analysis of Advanced Ovarian Cancer Tissue to Identify Potential Biomarkers of Responders and Nonresponders to First-Line Chemotherapy of Carboplatin and Paclitaxel.
abstract: Conventional treatment for advanced ovarian cancer is an initial debulking surgery followed by chemotherapy combination of carboplatin and paclitaxel. Despite initial high response, three-fourths of these women experience disease recurrence with a dismal prognosis. Patients with advanced-stage ovarian cancer who underwent cytoreductive surgery were enrolled and tissue samples were collected. Post surgery, these patients were started on chemotherapy and followed up till the end of the cycle. Fluorescence-based differential in-gel expression coupled with mass spectrometric analysis was used for discovery phase of experiments, and real-time polymerase chain reaction, Western blotting, and pathway analysis were performed for expression and functional validation of differentially expressed proteins. While aldehyde reductase, hnRNP, cyclophilin A, heat shock protein-27, and actin are upregulated in responders, prohibitin, enoyl-coA hydratase, peroxiredoxin, and fibrin-β are upregulated in the nonresponders. The expressions of some of these proteins correlated with increased apoptotic activity in responders and decreased apoptotic activity in nonresponders. Therefore, the proteins qualify as potential biomarkers to predict chemotherapy response.
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: Urmila
authors.initials: U
authors.lastname: Sehrawat
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: Ruchika
authors.initials: R
authors.lastname: Pokhriyal
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: Ashish Kumar
authors.initials: AK
authors.lastname: Gupta
authors.affiliation: Department of Medical Oncology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: Roopa
authors.initials: R
authors.lastname: Hariprasad
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: Mohd Imran
authors.initials: MI
authors.lastname: Khan
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: Divya
authors.initials: D
authors.lastname: Gupta
authors.affiliation: National Dairy Research Institute, Karnal, India.
authors.forename: Jasmine
authors.initials: J
authors.lastname: Naru
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: Sundararajan Baskar
authors.initials: SB
authors.lastname: Singh
authors.affiliation: National Dairy Research Institute, Karnal, India.
authors.forename: Ashok Kumar
authors.initials: AK
authors.lastname: Mohanty
authors.affiliation: Department of Obstetrics and Gynecology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: Perumal
authors.initials: P
authors.lastname: Vanamail
authors.affiliation: Department of Medical Oncology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: Lalit
authors.initials: L
authors.lastname: Kumar
authors.affiliation: Department of Obstetrics and Gynecology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: Sunesh
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: Gururao
authors.initials: G
authors.lastname: Hariprasad
keywords: advanced ovarian cancer; apoptotic pathways; biomarkers; chemotherapy response; gel-based proteomics