title: Construction of retroviral vector carrying HSV-tk gene under control of human AFP enhancer core sequence and human pgk promotor.
abstract: OBJECTIVE
  To construct retroviral vector bringing HSV-tk gene under control of human AFP enhancer core sequence and human pgk promoter.
  
  
  METHODS
  Internal SV40 promoter was deleted by SalI from retroviral vector pMNSM to construct pMNM. HSV-tk gene driven by pgk promoter was released by BamH I from an eukaryotic expression vector pBPGK-tk, and inserted into polylinker site of pMNM to construct pMNP-tk retroviral vector. Human α-fetoprotein gene enhancer core sequence was released by EcoR I from pGEM. 7Z-AFPe plasmid was inserted into the immediate upstream of pgk promoter of pMNP-tk vector. Construction of hepatoma specific retroviral vector pMNAP-tk was completed.
  
  
  RESULTS
  The structure of pMNP-tk and pMNAP-tk vector was confirmed by restriction analysis.
  
  
  CONCLUSIONS
  The vector is of great significance for hepatoma specific prodrug transformation gene therapy.
authors.affiliation: Jun Gao, Guang-Wen Cao, Zhong-Tian Qi, Xiao-Fang Qiu, Zhong-Di Wu, Ping Du, Wen-Guo Yang, Department of Microbiology, Faculty of Basic Medical Sciences, the Second Military Medical University, Shanghai 200433, China.
authors.forename: J
authors.initials: J
authors.lastname: Gao
authors.affiliation: Jun Gao, Guang-Wen Cao, Zhong-Tian Qi, Xiao-Fang Qiu, Zhong-Di Wu, Ping Du, Wen-Guo Yang, Department of Microbiology, Faculty of Basic Medical Sciences, the Second Military Medical University, Shanghai 200433, China.
authors.forename: G W
authors.initials: GW
authors.lastname: Cao
authors.affiliation: Jun Gao, Guang-Wen Cao, Zhong-Tian Qi, Xiao-Fang Qiu, Zhong-Di Wu, Ping Du, Wen-Guo Yang, Department of Microbiology, Faculty of Basic Medical Sciences, the Second Military Medical University, Shanghai 200433, China.
authors.forename: Z T
authors.initials: ZT
authors.lastname: Qi
authors.affiliation: Jun Gao, Guang-Wen Cao, Zhong-Tian Qi, Xiao-Fang Qiu, Zhong-Di Wu, Ping Du, Wen-Guo Yang, Department of Microbiology, Faculty of Basic Medical Sciences, the Second Military Medical University, Shanghai 200433, China.
authors.forename: X F
authors.initials: XF
authors.lastname: Qiu
authors.affiliation: Jun Gao, Guang-Wen Cao, Zhong-Tian Qi, Xiao-Fang Qiu, Zhong-Di Wu, Ping Du, Wen-Guo Yang, Department of Microbiology, Faculty of Basic Medical Sciences, the Second Military Medical University, Shanghai 200433, China.
authors.forename: Z D
authors.initials: ZD
authors.lastname: Wu
authors.affiliation: Jun Gao, Guang-Wen Cao, Zhong-Tian Qi, Xiao-Fang Qiu, Zhong-Di Wu, Ping Du, Wen-Guo Yang, Department of Microbiology, Faculty of Basic Medical Sciences, the Second Military Medical University, Shanghai 200433, China.
authors.forename: P
authors.initials: P
authors.lastname: Du
authors.affiliation: Jun Gao, Guang-Wen Cao, Zhong-Tian Qi, Xiao-Fang Qiu, Zhong-Di Wu, Ping Du, Wen-Guo Yang, Department of Microbiology, Faculty of Basic Medical Sciences, the Second Military Medical University, Shanghai 200433, China.
authors.forename: W G
authors.initials: WG
authors.lastname: Yang
authors.affiliation: Jun Gao, Guang-Wen Cao, Zhong-Tian Qi, Xiao-Fang Qiu, Zhong-Di Wu, Ping Du, Wen-Guo Yang, Department of Microbiology, Faculty of Basic Medical Sciences, the Second Military Medical University, Shanghai 200433, China.
authors.forename: L
authors.initials: L
authors.lastname: Cui
keywords: Alpha fetoproteins; Enhancer elements; Gene therapy; Herpes; Liver neoplasms; Retroviral; Simplex virus