title: EFFECTS OF HIGH PRESSURE IN THE ARMORED DINOFLAGELLATE SCRIPPSIELLA HEXAPRAECINGULA (PERIDINIALES, DINOPHYCEAE): CHANGES IN THECAL PLATE PATTERN AND MICROTUBULE ASSEMBLY(1).
abstract: The possible role of cortical microtubules in dinoflagellates was studied using high-pressure treatments applied to nonmotile cells (just after ecdysis) of Scrippsiella hexapraecingula T. Horig. et Chihara. Whereas considerable disorganization of cortical microtubules was observed when cells were exposed to high-pressure treatments of 98 MPa or more for 5-15 min, they were mostly intact in cells exposed to a pressure of <98 MPa for 5 min. After nonmotile cells were exposed to high-pressure treatments sufficient to disorganize the cortical microtubules, they produced new motile cells with thecal plate patterns that differed considerably from the pattern known for this species. Increasing the intensity of high pressure applied to nonmotile cells resulted in an increase in the number of cells that exhibited disorganized cortical microtubules as well as a change in their thecal plate pattern, suggesting that high pressure disorganizes cortical microtubules leading to a change in the thecal plate pattern.
authors.affiliation: Graduate School of Science, Kochi University, Kochi 780-8520, JapanGraduate School of Kuroshio Science, Kochi University, Kochi 780-8520, JapanDivision of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, 060-0810, JapanGraduate School of Kuroshio Science, Kochi University, Kochi 780-8520, Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Sekida
authors.affiliation: Graduate School of Science, Kochi University, Kochi 780-8520, JapanGraduate School of Kuroshio Science, Kochi University, Kochi 780-8520, JapanDivision of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, 060-0810, JapanGraduate School of Kuroshio Science, Kochi University, Kochi 780-8520, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Takahira
authors.affiliation: Graduate School of Science, Kochi University, Kochi 780-8520, JapanGraduate School of Kuroshio Science, Kochi University, Kochi 780-8520, JapanDivision of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, 060-0810, JapanGraduate School of Kuroshio Science, Kochi University, Kochi 780-8520, Japan.
authors.forename: Takeo
authors.initials: T
authors.lastname: Horiguchi
authors.affiliation: Graduate School of Science, Kochi University, Kochi 780-8520, JapanGraduate School of Kuroshio Science, Kochi University, Kochi 780-8520, JapanDivision of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, 060-0810, JapanGraduate School of Kuroshio Science, Kochi University, Kochi 780-8520, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Okuda
keywords: Scrippsiella hexapraecingula; cell covering; dinoflagellate; high pressure; microtubule; tabulation; thecal plate