title: A novel label-free and reusable electrochemical cytosensor for highly sensitive detection and specific collection of CTCs.
abstract: Circulating tumor cells (CTCs) contain a great deal of information of tumor phenotype. Therefore, highly sensitive detection and specific enrichment of CTCs are of intense interest. Herein, a label-free electrochemical impedance spectroscopy cytosensor with effective surface recognition between specific epithelial cell adhesion molecules (EpCAM) over-expressed on the cell membrane and EpCAM aptamer was developed for the detection of CTCs. After immobilization of 6-mercapto-1-hexanol (MCH) onto the gold electrode, the capture probe can be directionally inserted in MCH interspaces, which can improve the sensitivity of the cytosensor. A wide detection range from 30 to 1×10(6)cellsmL(-1) with a detection limit as low as 10cellsmL(-1) is reached on the condition of acceptable stability and reproducibility. The cytosensor can easily distinguish CTCs from the real blood sample due to the specific combination of EpCAM and EpCAM aptamer. Furthermore, the cytosensor can be reused 8 times and enrich CTCs by Uracil DNA Excision Mix specific cleaving the deoxyuridines (dUs) of the aptamer. The collected CTCs can contribute to further study. Thus, we reported that this cytosensor is a promising technique for the early monitoring and therapy of cancer.
authors.affiliation: Key Laboratory of Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Huawei
authors.initials: H
authors.lastname: Shen
authors.affiliation: Key Laboratory of Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Clinical Laboratory, Chongqing jiulongpo First People's Hospital, Chongqing 400050, PR China.
authors.forename: Zhongping
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Key Laboratory of Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Xueping
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Medical Laboratory, Chongqing Emergency Medical Center (The Fourth People's Hospital of Chongqing), Chongqing 400014, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.affiliation: Key Laboratory of Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Feihu
authors.initials: F
authors.lastname: Ji
authors.affiliation: Key Laboratory of Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China. Electronic address: guomingxie@cqmu.edu.cn.
authors.forename: Guoming
authors.initials: G
authors.lastname: Xie
authors.affiliation: Key Laboratory of Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China. Electronic address: fengwlcqmu@sina.com.
authors.forename: Wenli
authors.initials: W
authors.lastname: Feng
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002452:Cell Count / Q000379:methods*; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods; D058266:Dielectric Spectroscopy / Q000379:methods*; D000071858:Epithelial Cell Adhesion Molecule / Q000032:analysis; D006801:Humans; D009360:Neoplastic Cells, Circulating / Q000473:pathology*
chemical_list: D052157:Aptamers, Nucleotide; D000071858:Epithelial Cell Adhesion Molecule
keywords: Circulating tumor cells; Collection; Electrochemical cytosensor; Label-free; Reuse