title: Improved thermostable polyvinyl alcohol electrospun nanofibers with entangled naringinase used in a novel mini-packed bed reactor.
abstract: Polyvinyl alcohol (PVA) electrospun nanofibers were produced using an electrospinning technique. Key parameters (e.g. collectors, distance from needle tip to collector, among others) that influence the structure and morphology of fibers were optimized. The naringinase entrapped in PVA nanofibers retained over 100% of its initial activity after 212h of operation, at 25°C. Chemical crosslinking with several boronic acids further increased the hydrolysis temperature (up to 85°C) and yielded nanofibers with thermal stability up to 121°C. A mini packed bed reactor (PBR) developed to establish the feasibility for continuous enzymatic operation, ran for 16days at 45°C. Highest naringenin biosynthesis was attained at a flow rate of 10mLh(-1). Highest volumetric (78molL(-1)h(-1)) and specific (26molh(-1)genzyme(-1)) productivities were attained at 30mLh(-1). The activity of NGase in electrospun nanofibers remained constant for almost 16days of operation at 10mLh(-1).
authors.affiliation: Research Institute for Medicines (iMed.ULisboa), Faculdade de Farmácia, Universidade Lisboa, Av. Gama Pinto, 1649-003 Lisboa, Portugal.
authors.forename: Mário A P
authors.initials: MAP
authors.lastname: Nunes
authors.affiliation: Research Institute for Medicines (iMed.ULisboa), Faculdade de Farmácia, Universidade Lisboa, Av. Gama Pinto, 1649-003 Lisboa, Portugal.
authors.forename: Samuel
authors.initials: S
authors.lastname: Martins
authors.affiliation: Instituto de Ciência e Engenharia de Materiais e Superfícies, Departamento de Engenharia de Materiais, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.
authors.forename: M Emilia
authors.initials: ME
authors.lastname: Rosa
authors.affiliation: Research Institute for Medicines (iMed.ULisboa), Faculdade de Farmácia, Universidade Lisboa, Av. Gama Pinto, 1649-003 Lisboa, Portugal.
authors.forename: Pedro M P
authors.initials: PMP
authors.lastname: Gois
authors.affiliation: Institute of Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.
authors.forename: Pedro C B
authors.initials: PCB
authors.lastname: Fernandes
authors.affiliation: Research Institute for Medicines (iMed.ULisboa), Faculdade de Farmácia, Universidade Lisboa, Av. Gama Pinto, 1649-003 Lisboa, Portugal. Electronic address: mhribeiro@ff.ulisboa.pt.
authors.forename: Maria H L
authors.initials: MHL
authors.lastname: Ribeiro
mesh_terms: D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods; D001897:Boronic Acids / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D044950:Flavanones / Q000096:biosynthesis; D006868:Hydrolysis; D009097:Multienzyme Complexes / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D013696:Temperature; D001617:beta-Glucosidase / Q000737:chemistry*
chemical_list: D001897:Boronic Acids; D003432:Cross-Linking Reagents; D044950:Flavanones; D009097:Multienzyme Complexes; D011142:Polyvinyl Alcohol; C039827:naringinase; D001617:beta-Glucosidase; C005273:naringenin
keywords: Electrospun nanofibers; Mini-packed bed reactor; Naringinase; Operational stability; Polyvinyl alcohol (PVA)