title: Cloning, Expression Profiling and Functional Analysis of CnHMGS, a Gene Encoding 3-hydroxy-3-Methylglutaryl Coenzyme A Synthase from Chamaemelum nobile.
abstract: Roman chamomile (Chamaemelum nobile L.) is renowned for its production of essential oils, which major components are sesquiterpenoids. As the important enzyme in the sesquiterpenoid biosynthesis pathway, 3-hydroxy-3-methylglutaryl coenzyme A synthase (HMGS) catalyze the crucial step in the mevalonate pathway in plants. To isolate and identify the functional genes involved in the sesquiterpene biosynthesis of C. nobile L., a HMGS gene designated as CnHMGS (GenBank Accession No. KU529969) was cloned from C. nobile. The cDNA sequence of CnHMGS contained a 1377 bp open reading frame encoding a 458-amino-acid protein. The sequence of the CnHMGS protein was highly homologous to those of HMGS proteins from other plant species. Phylogenetic tree analysis revealed that CnHMGS clustered with the HMGS of Asteraceae in the dicotyledon clade. Further functional complementation of CnHMGS in the mutant yeast strain YSC6274 lacking HMGS activity demonstrated that the cloned CnHMGS cDNA encodes a functional HMGS. Transcript profile analysis indicated that CnHMGS was preferentially expressed in flowers and roots of C. nobile. The expression of CnHMGS could be upregulated by exogenous elicitors, including methyl jasmonate and salicylic acid, suggesting that CnHMGS was elicitor-responsive. The characterization and expression analysis of CnHMGS is helpful to understand the biosynthesis of sesquiterpenoid in C. nobile at the molecular level and also provides molecular wealth for the biotechnological improvement of this important medicinal plant.
authors.affiliation: School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China. s_y_cheng@sina.com.
authors.forename: Shuiyuan
authors.initials: S
authors.lastname: Cheng
authors.affiliation: College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei, China. wxhunique@foxmail.com.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei, China. xufeng198@126.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.affiliation: College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei, China. chenqwx@foxmail.com.
authors.forename: Qiangwen
authors.initials: Q
authors.lastname: Chen
authors.affiliation: College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei, China. taotaoo2o3@foxmail.com.
authors.forename: Tingting
authors.initials: T
authors.lastname: Tao
authors.affiliation: College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei, China. 13872337364@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Lei
authors.affiliation: College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei, China. zww8312@163.com.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei, China. liaoyongling@yeah.net.
authors.forename: Yongling
authors.initials: Y
authors.lastname: Liao
authors.affiliation: Hubei Collaborative Innovation Center of Targeted Antitumor Drug, Jingchu University of Technology, Jingmen 448000, Hubei, China. 18986662379@163.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Chang
authors.affiliation: Medical School, Yangtze University, Jingzhou 434025, Hubei, China. lxx@yangtzeu.edu.cn.
authors.forename: Xingxiang
authors.initials: X
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D019659:Asteraceae / Q000235:genetics; D001483:Base Sequence; D031167:Chamaemelum / Q000201:enzymology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D006904:Hydroxymethylglutaryl-CoA Synthase / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D008798:Mevalonic Acid / Q000378:metabolism; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D006904:Hydroxymethylglutaryl-CoA Synthase; D008798:Mevalonic Acid
keywords: 3-hydroxy-3-methylglutaryl coenzyme A synthase; Chamaemelum nobile; cloning; expression profile; functional complementation analysis; sesquiterpenoid biosynthesis