title: Immunohistochemical Expression of GLUT-1 and HIF-1α in Tooth Germ, Ameloblastoma, and Ameloblastic Carcinoma.
abstract: Hypoxia-inducible factor-1α (HIF-1α) promotes proteins that enable cell survival during hypoxia, such as glucose transporter 1 (GLUT-1). Their coexpression has been associated with aggressiveness in malignancies and has not been studied in odontogenic tumors. Immunohistochemical expression of HIF-1α and GLUT-1 was analyzed in 13 tooth germs (TGs), 55 ameloblastomas (AMs), and 3 ameloblastic carcinomas (ACs). HIF-1α was negative in all TGs, and just 1 case of AM and 1 of AC had nuclear positivity. GLUT-1 expressed in ameloblastic cells of all TGs, AMs, and ACs, with an increasing intensity, respectively, and was significantly higher in solid AM than in unicystic AM (P = .041). Absence of nuclear HIF-1α in TGs and most AMs suggest that GLUT-1 may be induced by alternative pathways to hypoxia. However, in ACs, HIF-1α may be activated; however, to confirm this, additional cases are needed. GLUT-1 overexpression could be related to aggressiveness in AMs and ACs and must represent a normal metabolite in TGs.
authors.affiliation: University of Campinas (UNICAMP), São Paulo, Brazil csr_90@hotmail.com.
authors.forename: Celeste
authors.initials: C
authors.lastname: Sánchez-Romero
authors.affiliation: Universidad de La República (UDELAR), Montevideo, Uruguay.
authors.forename: Ronell
authors.initials: R
authors.lastname: Bologna-Molina
authors.affiliation: Universidad Autónoma Metropolitana Xochimilco, Mexico City, Mexico.
authors.forename: Adalberto
authors.initials: A
authors.lastname: Mosqueda-Taylor
authors.affiliation: University of Campinas (UNICAMP), São Paulo, Brazil.
authors.forename: Oslei
authors.initials: O
authors.lastname: Paes de Almeida
mesh_terms: D000564:Ameloblastoma / Q000175:diagnosis / Q000473:pathology; D014408:Biomarkers, Tumor / Q000032:analysis*; D002277:Carcinoma / Q000175:diagnosis / Q000473:pathology; D051272:Glucose Transporter Type 1 / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000032:analysis / Q000096:biosynthesis*; D007090:Image Interpretation, Computer-Assisted; D007150:Immunohistochemistry; D007573:Jaw Neoplasms / Q000175:diagnosis* / Q000473:pathology; D009808:Odontogenic Tumors / Q000175:diagnosis* / Q000473:pathology; D014083:Tooth Germ / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D051272:Glucose Transporter Type 1; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; C495677:SLC2A1 protein, human
keywords: GLUT-1; HIF-1α; ameloblastic carcinoma; ameloblastoma; odontogenic tumors