title: MiR-301a is involved in adipocyte dysfunction during obesity-related inflammation via suppression of PPARγ.
abstract: The present study explored the involvement and role of miR-301a in the adipose tissues. For the first time we identified the expression of miR-301a in the white adipose tissues of mice. A decreased level of miR-301a was correlated to increased chronic inflammation in the 3T3-L1 cells and circula- tion in an obese mouse model. Mechanistically, we demonstrated that miR-301a attenuated saturated free fatty acid-induced activation of peroxisome proliferator-activated receptor gamma (PPARy) and production of proinflammatory cytokines in 3T3-L1 cells. Target gene reporter assays showed that miR-301a directly targeted the 3'-untranslated region (3'UTR) of PPARγ, resulting in a decrease of PPARy protein expression. The miR-301a inhibition of adipocyte differentiation was reversed by PPARγ overexpression.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Mei
authors.initials: M
authors.lastname: Xue
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Qin
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D041721:3T3-L1 Cells; D017667:Adipocytes / Q000473:pathology*; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D016207:Cytokines / Q000378:metabolism; D017930:Genes, Reporter / Q000235:genetics; D007249:Inflammation / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics*; D009765:Obesity / Q000473:pathology*; D047495:PPAR gamma / Q000235:genetics*; D013489:Suppression, Genetic
chemical_list: D020413:3' Untranslated Regions; D016207:Cytokines; C573832:MIRN301 microRNA, mouse; D035683:MicroRNAs; D047495:PPAR gamma