title: Domestication Syndrome Is Investigated by Proteomic Analysis between Cultivated Cassava (Manihot esculenta Crantz) and Its Wild Relatives.
abstract: Cassava (Manihot esculenta Crantz) wild relatives remain a largely untapped potential for genetic improvement. However, the domestication syndrome phenomena from wild species to cultivated cassava remain poorly understood. The analysis of leaf anatomy and photosynthetic activity showed significantly different between cassava cultivars SC205, SC8 and wild relative M. esculenta ssp. Flabellifolia (W14). The dry matter, starch and amylose contents in the storage roots of cassava cultivars were significantly more than that in wild species. In order to further reveal the differences in photosynthesis and starch accumulation of cultivars and wild species, the globally differential proteins between cassava SC205, SC8 and W14 were analyzed using 2-DE in combination with MALDI-TOF tandem mass spectrometry. A total of 175 and 304 proteins in leaves and storage roots were identified, respectively. Of these, 122 and 127 common proteins in leaves and storage roots were detected in SC205, SC8 and W14, respectively. There were 11, 2 and 2 unique proteins in leaves, as well as 58, 9 and 12 unique proteins in storage roots for W14, SC205 and SC8, respectively, indicating proteomic changes in leaves and storage roots between cultivated cassava and its wild relatives. These proteins and their differential regulation across plants of contrasting leaf morphology, leaf anatomy pattern and photosynthetic related parameters and starch content could contribute to the footprinting of cassava domestication syndrome. We conclude that these global protein data would be of great value to detect the key gene groups related to cassava selection in the domestication syndrome phenomena.
authors.affiliation: Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Ministry of Agriculture for Germplasm Resources Conservation and Utilization of Cassava, Danzhou 571737, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: An
authors.affiliation: Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Ministry of Agriculture for Germplasm Resources Conservation and Utilization of Cassava, Danzhou 571737, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Chen
authors.affiliation: Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Ministry of Agriculture for Germplasm Resources Conservation and Utilization of Cassava, Danzhou 571737, China.|Laboratory of Plant Physiology, Higher Teacher's Training College, University of Yaounde I, P. O. Box 47, Yaounde, Cameroon.
authors.forename: Djabou Mouafi Astride
authors.initials: DM
authors.lastname: Stéphanie
authors.affiliation: Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Ministry of Agriculture for Germplasm Resources Conservation and Utilization of Cassava, Danzhou 571737, China.
authors.forename: Kaimian
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Manoa, HI 96822, United States of America.
authors.forename: Qing X
authors.initials: QX
authors.lastname: Li
authors.affiliation: Genetic Resources and Biotechnology, Embrapa, Brasilia-DF 02372, Brazil.
authors.forename: Luiz J C B
authors.initials: LJ
authors.lastname: Carvalho
authors.affiliation: Natural Resources Institute, University of Greenwich, Chatham ME4 4TB, United Kingdom.
authors.forename: Keith
authors.initials: K
authors.lastname: Tomlins
authors.affiliation: Analysis and Testing Center, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Chemical Starch Institute, Guangxi University, Nanning 300004, China.
authors.forename: Bi
authors.initials: B
authors.lastname: Gu
authors.affiliation: Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Ministry of Agriculture for Germplasm Resources Conservation and Utilization of Cassava, Danzhou 571737, China.
authors.forename: Songbi
authors.initials: S
authors.lastname: Chen
mesh_terms: D000687:Amylopectin / Q000378:metabolism; D000688:Amylose / Q000378:metabolism; D015153:Blotting, Western; D018556:Crops, Agricultural / Q000254:growth & development* / Q000378:metabolism*; D002365:Manihot / Q000033:anatomy & histology / Q000254:growth & development* / Q000378:metabolism*; D010788:Photosynthesis; D018515:Plant Leaves / Q000033:anatomy & histology / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D060066:Protein Interaction Maps; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013213:Starch / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D013213:Starch; D000688:Amylose; D000687:Amylopectin