title: Phosphoproteome Analysis of Invasion and Metastasis-Related Factors in Pancreatic Cancer Cells.
abstract: Mechanisms of abnormal protein phosphorylation that regulate cell invasion and metastasis in pancreatic cancer remain obscure. In this study, we used high-throughput phosphorylation array to test two pancreatic cancer cell lines (PC-1 cells with a low, and PC-1.0 cells with a high potential for invasion and metastasis). We noted that a total of 57 proteins revealed a differential expression (fold change ≥ 2.0). Six candidate proteins were further validated by western blot with results found to be accordance with the array. Of 57 proteins, 32 up-regulated proteins (e.g. CaMK1-α and P90RSK) were mainly involved in ErbB and neurotrophin signaling pathways as determined using DAVID software, while 25 down-regulated proteins (e.g. BID and BRCA1) were closely involved in apoptosis and p53 signaling pathways. Moreover, four proteins (AKT1, Chk2, p53 and P70S6K) with different phosphorylation sites were found, not only among up-regulated, but also among down-regulated proteins. Importantly, specific phosphorylation sites can affect cell biological functions. CentiScaPe software calculated topological characteristics of each node in the protein-protein interaction (PPI) network: we found that AKT1 owns the maximum node degrees and betweenness in the up-regulation protein PPI network (26 nodes, average path length: 1.89, node degrees: 6.62±4.18, betweenness: 22.23±35.72), and p53 in the down-regulation protein PPI network (17 nodes, average path length: 2.04, node degrees: 3.65±2.47, betweenness: 16.59±29.58). In conclusion, the identification of abnormal protein phosphorylation related to invasion and metastasis may allow us to identify new biomarkers in an effort to develop novel therapeutic drug targets for pancreatic cancer treatment.
authors.affiliation: Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning, P.R.China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Tan
authors.affiliation: Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning, P.R.China.
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.affiliation: Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning, P.R.China.
authors.forename: Yinpeng
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning, P.R.China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning, P.R.China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning, P.R.China.
authors.forename: Huaitao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning, P.R.China.
authors.forename: Boqiang
authors.initials: B
authors.lastname: Yu
authors.affiliation: Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning, P.R.China.
authors.forename: Xiangli
authors.initials: X
authors.lastname: Meng
authors.affiliation: Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning, P.R.China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning, P.R.China.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology; D010766:Phosphorylation / Q000235:genetics; D060066:Protein Interaction Maps / Q000235:genetics*; D020543:Proteome / Q000235:genetics*; D015398:Signal Transduction
chemical_list: D009363:Neoplasm Proteins; D020543:Proteome