title: Differential expression of circulating microRNAs in blood and haematoma samples from patients with intracerebral haemorrhage.
abstract: OBJECTIVE
  To measure the differential expression of microRNAs (miRNAs) in peripheral blood samples from patients with intracerebral haemorrhage (ICH) and to measure the levels of hsa-miR-21-5p in peripheral blood and haematoma samples from patients with ICH.
  
  
  METHODS
  This case-control study enrolled individuals with ICH in the putamen treated by craniotomy and age- and sex-matched healthy control subjects. Serum miRNA expression profiles were determined in the patient and control groups using miRNA polymerase chain reaction (PCR) arrays. The ICH-related miRNA hsa-miR-21-5p was selected and its differential expression was assessed in peripheral blood and haematoma specimens from patients with ICH compared with peripheral blood samples controls using real-time PCR.
  
  
  RESULTS
  Seven patients and five control subjects were included in the miRNA expression profile analysis; and 31 patients and 22 control subjects provided samples for the real-time PCR of hsa-miR-21-5p expression. A total of 59 miRNAs were significantly downregulated in patients with ICH. Relative hsa-miR-21-5p levels of 0.43 and 0.31 for peripheral blood and haematoma samples, respectively, were obtained in the patient group compared with the control subjects.
  
  
  CONCLUSIONS
  Hsa-miR-21-5p levels were significantly reduced in both peripheral blood and haematoma samples in patients with ICH.
authors.affiliation: Department of Neurology, First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Jialu
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Neurology, First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Neurology, First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Jin
authors.affiliation: Department of Neurology, First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Tang
authors.affiliation: Department of Neurology, First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Zhenwei
authors.initials: Z
authors.lastname: He
authors.affiliation: Department of Neurology, First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China hezhiyi0301@sina.com.
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: He
mesh_terms: D016022:Case-Control Studies; D002543:Cerebral Hemorrhage / Q000097:blood* / Q000150:complications*; D003710:Demography; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006406:Hematoma / Q000097:blood* / Q000150:complications / Q000235:genetics*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics*; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D035683:MicroRNAs
keywords: Intracerebral haemorrhage; haematoma; has-miR-21-5p; microRNA; microRNA polymerase chain reaction array; peripheral blood