title: The IncP-6 Plasmid p10265-KPC from Pseudomonas aeruginosa Carries a Novel ΔISEc33-Associated bla KPC-2 Gene Cluster.
abstract: Pseudomonas aeruginosa strain 10265 was recovered from a patient with pneumonia in a Chinese public hospital, and it displays the carbapenem resistance phenotype due to the acquisition of a non-conjugative but mobilizable IncP-6-type plasmid p10265-KPC. p10265-KPC carries a Tn5563-borne defective mer locus, and a novel ΔISEc33-associated bla KPC-2 gene cluster without paired inverted repeats and paired direct repeats at both ends. Mobilization of this ΔISEc33-associated element in p10265-KPC would be attributed to homologous recombination-based insertion of a foreign structure Tn3-ISApu1-orf7-ISApu2- ISKpn27-Δbla TEM-1 -bla KPC-2 -ΔISKpn6- korC-orf6-klcA-ΔrepB into a pre-existent intact ISEc33, making ISEc33 truncated at the 3' end. The previously reported pCOL-1 represents the first sequenced KPC-producing IncP-6 plasmid, while p10265-KPC is the second one. These two plasmids carry two distinct bla KPC-2 gene clusters, which are inserted into the different sites of the IncP-6 backbone and have different evolutionary histories of assembly and mobilization. This is the first report of identification of the IncP-6-type resistance plasmid in China.
authors.affiliation: Department of Pulmonology, Southwest Hospital, Third Military Medical University Chongqing, China.
authors.forename: Xiaotian
authors.initials: X
authors.lastname: Dai
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology Beijing, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhou
authors.affiliation: Department of Pulmonology, Southwest Hospital, Third Military Medical University Chongqing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xiong
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology Beijing, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and EpidemiologyBeijing, China; Department of Pharmacy, Southwest Hospital, Third Military Medical UniversityChongqing, China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Luo
authors.affiliation: Department of Pulmonology, Southwest Hospital, Third Military Medical University Chongqing, China.
authors.forename: Guangming
authors.initials: G
authors.lastname: Luo
authors.affiliation: Department of Pulmonology, Southwest Hospital, Third Military Medical University Chongqing, China.
authors.forename: Haijing
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University Chongqing, China.
authors.forename: Fengjun
authors.initials: F
authors.lastname: Sun
authors.affiliation: Department of Pulmonology, Southwest Hospital, Third Military Medical University Chongqing, China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Zhou
keywords: IncP-6; KPC-2; Pseudomonas aeruginosa; p10265-KPC