title: Paraoxonase 1 (PON1) and pomegranate influence circadian gene expression and period length.
abstract: The circadian timing system regulates key aspects of mammalian physiology. Here, we analyzed the effect of the endogenous antioxidant paraoxonase 1 (PON1), a high-density lipoprotein-associated lipolactonase that hydrolyses lipid peroxides and attenuates atherogenesis, on circadian gene expression in C57BL/6J and PON1KO mice fed a normal chow diet or a high-fat diet (HFD). Expression levels of core-clock transcripts Nr1d1, Per2, Cry2 and Bmal1 were altered in skeletal muscle in PON1-deficient mice in response to HFD. These findings were supported by circadian bioluminescence reporter assessments in mouse C2C12 and human primary myotubes, synchronized in vitro, where administration of PON1 or pomegranate juice modulated circadian period length.
authors.affiliation: a Division of Endocrinology, Diabetes and Nutrition, Department of Clinical Medicine, Faculty of Medicine , University of Geneva , Geneva , Switzerland.
authors.forename: Ursula
authors.initials: U
authors.lastname: Loizides-Mangold
authors.affiliation: b The Lipid Research Laboratory, Technion Faculty of Medicine , the Rappaport Family Institute for Research in the Medical Sciences, and Rambam Medical Center , Haifa , Israel.
authors.forename: Marie
authors.initials: M
authors.lastname: Koren-Gluzer
authors.affiliation: a Division of Endocrinology, Diabetes and Nutrition, Department of Clinical Medicine, Faculty of Medicine , University of Geneva , Geneva , Switzerland.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Skarupelova
authors.affiliation: a Division of Endocrinology, Diabetes and Nutrition, Department of Clinical Medicine, Faculty of Medicine , University of Geneva , Geneva , Switzerland.
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Makhlouf
authors.affiliation: b The Lipid Research Laboratory, Technion Faculty of Medicine , the Rappaport Family Institute for Research in the Medical Sciences, and Rambam Medical Center , Haifa , Israel.
authors.forename: Tony
authors.initials: T
authors.lastname: Hayek
authors.affiliation: b The Lipid Research Laboratory, Technion Faculty of Medicine , the Rappaport Family Institute for Research in the Medical Sciences, and Rambam Medical Center , Haifa , Israel.
authors.forename: Michael
authors.initials: M
authors.lastname: Aviram
authors.affiliation: a Division of Endocrinology, Diabetes and Nutrition, Department of Clinical Medicine, Faculty of Medicine , University of Geneva , Geneva , Switzerland.
authors.forename: Charna
authors.initials: C
authors.lastname: Dibner
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D043303:Aryldialkylphosphatase / Q000235:genetics*; D002478:Cells, Cultured; D057906:Circadian Clocks / Q000235:genetics*; D002940:Circadian Rhythm / Q000235:genetics* / Q000502:physiology; D059305:Diet, High-Fat; D015870:Gene Expression / Q000235:genetics*; D029561:Lythraceae / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D000975:Antioxidants; D012333:RNA, Messenger; D043303:Aryldialkylphosphatase; C560867:PON1 protein, mouse
keywords: Circadian bioluminescence recording; PON1; circadian clock; pomegranate; skeletal muscle