title: Cloning and identification of the lobophorin biosynthetic gene cluster from marine Streptomyces olivaceus strain FXJ7.023.
abstract: A full length about 105 kb gene cluster containing 35 open reading frames involved in the biosynthesis of lobophorins was cloned and sequenced from a fosmid genomic library of Streptomyces olivaceus strain FXJ7.023. The cluster was identified by genome wide annotation and analysis of secondary metabolite biosynthesis gene clusters by anti SMASH and knockout of loading module-contained region of polyketide skeleton synthesis gene (the starter of lobS1). Gene cluster comparative analysis suggested that the cluster encoded the complete genes for lobophorin polyketide assembly, modification, substrate catalysis, regulation, transportation and resistance, and shows great identity to the newest reported lobophorin biosynthetic gene cluster from Streptomyces sp. SCSIO 01127, but with a significant gene rearrangement in the PKS modules.
authors.affiliation: Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical College, Zunyi, Guizhou, China / State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Changwu
authors.initials: C
authors.lastname: Yue
authors.affiliation: Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical College, Zunyi, Guizhou, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Niu
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.affiliation: Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical College, Zunyi, Guizhou, China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Lü
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Minghao
authors.initials: M
authors.lastname: Liu
authors.affiliation: Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical College, Zunyi, Guizhou, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Li
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D005285:Fermentation; D055786:Gene Knockout Techniques; D015723:Gene Library; D018942:Macrolides / Q000378:metabolism*; D005810:Multigene Family / Q000235:genetics*; D010957:Plasmids; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D018942:Macrolides