title: Mycosynthesis of silver nanoparticles bearing antibacterial activity.
abstract: Mycosynthesis of silver nanoparticles was achieved by endophytic Colletotrichum sp. ALF2-6 inhabiting Andrographis paniculata. Well dispersed nanoparticles were characterized using UV-Visible spectrometry with maximum absorption conferring at 420 nm. FTIR analysis revealed possible biomolecules reducing the metal salt and stabilization of nanoparticles. XRD analysis depicted the diffraction intensities exhibiting between 20 and 80 °C at 2theta angle thus conferring the crystalline nature of nanoparticles. Morphological characteristic using TEM revealed the polydispersity of nanoparticles with size ranging from 20 to 50 nm. Synthesized nanoparticles exhibited bactericidal activity against selected human pathogens. Nanoparticles mode of action was carried out to reveal DNA damage activity. Thus the present investigation reports facile fabrication of silver nanoparticles from endophytic fungi.
authors.affiliation: Bionano Technological Laboratory, Department of Studies in Microbiology, University of Mysore, Manasagangotri, India.
authors.forename: Pasha
authors.initials: P
authors.lastname: Azmath
authors.affiliation: Bionano Technological Laboratory, Department of Studies in Microbiology, University of Mysore, Manasagangotri, India.
authors.forename: Syed
authors.initials: S
authors.lastname: Baker
authors.affiliation: Bionano Technological Laboratory, Department of Studies in Microbiology, University of Mysore, Manasagangotri, India.
authors.forename: Devaraju
authors.initials: D
authors.lastname: Rakshith
authors.affiliation: Bionano Technological Laboratory, Department of Studies in Microbiology, University of Mysore, Manasagangotri, India.
authors.forename: Sreedharamurthy
authors.initials: S
authors.lastname: Satish
keywords: Andrographis paniculata; Antibacterial activity; Colletotrichum sp.; Mycosynthesis; Nanoparticles