title: Detection of influenza virus using peroxidase-mimic of gold nanoparticles.
abstract: A modified enzyme-linked immunosorbent assay (ELISA) with nanomaterials is an effective and powerful method to amplify the signal and reduce the cost of detecting and measuring trace biomarkers or proteins. In this study, an ultra-sensitive colorimetric immunoassay was designed, and its ability to detect influenza viruses using positively charged gold nanoparticles ((+)Au NPs) was assessed as a possible role for peroxidase-mimic inorganic enzymes. This method detected influenza virus A (H1N1) with a linear range up to 10 pg mL(-1) and clinically isolated influenza virus A (H3N2) up to 10 plaque forming units (PFU) mL(-1) , where its sensitivity improved to 500-fold higher than that of commercial virus kits. The sensitivity of this proposed method was not declined even though in complex biological media in compared to conventional ELISA. These results revealed that the (+)AuNP-based colorimetric immunoassay could be suitable for lab-on-a-chip device and open new opportunities for clinical protein diagnostics. Biotechnol. Bioeng. 2016;113: 2298-2303. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Research Institute of Green Science and Technology, Shizuoka University, Ohya Suruga-ku, Shizuoka, Japan.
authors.forename: Syed Rahin
authors.initials: SR
authors.lastname: Ahmed
authors.affiliation: Department of Cogno-Mechatronics Engineering, Pusan National University, Busan, 609-735, Republic of Korea.
authors.forename: Jeonghyo
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Infectious Diseases, Hamamatsu University School of Medicine, Higashi-ku, Handa-yama, Hamamatsu, Japan.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: Department of Cogno-Mechatronics Engineering, Pusan National University, Busan, 609-735, Republic of Korea. jaebeom@pusan.ac.kr.
authors.forename: Jaebeom
authors.initials: J
authors.lastname: Lee
authors.affiliation: Research Institute of Green Science and Technology, Shizuoka University, Ohya Suruga-ku, Shizuoka, Japan. park.enoch@shizuoka.ac.jp.|Graduate School of Science and Technology, Shizuoka University, 836 Ohya Suruga-ku, Shizuoka, 422-8529, Japan. park.enoch@shizuoka.ac.jp.
authors.forename: Enoch Y
authors.initials: EY
authors.lastname: Park
mesh_terms: D001560:Benzidines / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation; D003124:Colorimetry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D053122:Influenza A Virus, H3N2 Subtype / Q000276:immunology / Q000302:isolation & purification*; D053768:Metal Nanoparticles / Q000737:chemistry*; D009195:Peroxidase / Q000737:chemistry* / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D001560:Benzidines; C021758:3,3',5,5'-tetramethylbenzidine; D006046:Gold; D006861:Hydrogen Peroxide; D009195:Peroxidase
keywords: gold nanoparticles; immunoassay; influenza virus; peroxidase-mimic enzymatic reaction