title: Morphology, proliferation, and gene expression of gingival fibroblasts on Laser-Lok, titanium, and zirconia surfaces.
abstract: Soft tissue seal plays a critical role in long-term success of dental implants, and the effects of implant surface treatments such as laser ablation have been a topic of particular interest in this respect. Considering the existing controversy regarding soft tissue behavior in contact with implant surfaces, this study sought to assess the morphology, proliferation, and gene expression of human gingival fibroblasts (HGFs) on different abutment surfaces. In this in vitro, experimental study, HGFs were cultured on 45 discs (Laser-Lok, titanium, and zirconia). Cell morphology, proliferation rate, and interleukin 10 (IL-10), tumor necrosis factor alpha (TNFα), fibronectin, and integrin gene expressions were assessed by electron microscopy, methyl thiazol tetrazolium (MTT) assay, and real-time polymerase chain reaction (PCR), respectively. Data were analyzed using ANOVA and the Kruskal-Wallis H test. Fibroblast attachment was noted in all the three groups. Spindle-shaped cells with pseudopod-like processes were more frequently seen in the Laser-Lok group. Cell proliferation was significantly higher in the Laser-Lok group compared to those in the other groups (P = 0.0002). Significant differences were found in the expression of IL-10, TNFα, fibronectin, and integrin genes among the groups (P < 0.01). Within the limitations of this study, HGFs on Laser-Lok surfaces had a more mature morphology and greater proliferation and differentiation as compared to those on zirconia and titanium surfaces. This indicates better attachment of these cells to laser-modified surfaces, creating a more efficient soft tissue seal around dental implants.
authors.affiliation: Department of Periodontics, Dental Branch, Islamic Azad University, Tehran, Iran.|Dental Implant Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Nasrin
authors.initials: N
authors.lastname: Esfahanizadeh
authors.affiliation: Department of Periodontics, Dental Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Sara
authors.initials: S
authors.lastname: Motalebi
authors.affiliation: , 1501 Harvey Rd, #586, College Station, TX, 77840, USA. nildan89@yahoo.com.
authors.forename: Niloufar
authors.initials: N
authors.lastname: Daneshparvar
authors.affiliation: Department of Mathematics, South Tehran Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Nasrin
authors.initials: N
authors.lastname: Akhoundi
authors.affiliation: National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Shahin
authors.initials: S
authors.lastname: Bonakdar
mesh_terms: D049109:Cell Proliferation / Q000528:radiation effects*; D002478:Cells, Cultured; D015921:Dental Implants; D005347:Fibroblasts / Q000528:radiation effects*; D005353:Fibronectins / Q000528:radiation effects; D015870:Gene Expression / Q000528:radiation effects*; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D028022:Low-Level Light Therapy / Q000295:instrumentation* / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction; D013499:Surface Properties; D014025:Titanium; D015040:Zirconium
chemical_list: D015921:Dental Implants; C067625:FN1 protein, human; D005353:Fibronectins; D015040:Zirconium; D014025:Titanium; C028541:zirconium oxide
keywords: Fibroblasts; Implant abutment; Laser-Lok; Titanium; Zirconia