title: Early application of negative pressure wound therapy to acute wounds contaminated withStaphylococcus aureus: An effective approach to preventing biofilm formation.
abstract: Negative pressure wound therapy (NPWT) has been demonstrated to be effective at preventing biofilm-associated infections; however, its role in biofilm prevention is unknown. The present study evaluated the effect of NPWT on biofilm prevention when rapidly initiated following wound contamination. Full-thickness dermal wounds (8 mm) were created in rabbit ears and inoculated with green fluorescent protein-labeledStaphylococcus aureus(S. aureus). At 6 h following inoculation, continuous NPWT at -125 mmHg was initiated, with the wounds on the contralateral ear left untreated in order to serve as self-controls.S. aureusrapidly formed mature biofilms in the wound beds post-inoculation, with a persistent bacterial burden of ~105-107colony-forming units (CFUs)/wound and impaired wound healing. Compared with the untreated group, NPWT resulted in a significant reduction in biofilm matrix, which was verified by scanning electron microscopy and epifluorescence. A reduction in bacterial counts followed (P<0.05) with ~103CFUs/wound on postoperative day 13 and improvement in all healing parameters (P<0.05) relative to control wounds. The results of the present investigation suggest that NPWT is an effective strategy to impeding the formation ofS. aureuswound biofilms when initiated rapidly following bacterial contamination. The early application of NPWT, aimed at biofilm prevention, may improve wound care.
authors.affiliation: Department of Clinical Medicine, Medical College, Nankai University, Tianjin 300071, P.R. China; Department of Orthopedics, Chinese PLA General Hospital, Beijing 100853, P.R. China.
authors.forename: Tongtong
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Chinese PLA General Hospital, Beijing 100853, P.R. China.
authors.forename: Lihai
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Center for Hospital Infection Control, Chinese PLA Institute for Disease Control and Prevention, Beijing 100071, P.R. China.
authors.forename: L I
authors.initials: LI
authors.lastname: Han
authors.affiliation: Department of Orthopedics, Chinese PLA General Hospital, Beijing 100853, P.R. China.
authors.forename: Guoqi
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Clinical Medicine, Medical College, Nankai University, Tianjin 300071, P.R. China; Department of Orthopedics, Chinese PLA General Hospital, Beijing 100853, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Yin
authors.affiliation: Department of Orthopedics, Chinese PLA General Hospital, Beijing 100853, P.R. China.
authors.forename: Zhirui
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Chinese PLA General Hospital, Beijing 100853, P.R. China.
authors.forename: Licheng
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Clinical Medicine, Medical College, Nankai University, Tianjin 300071, P.R. China; Department of Orthopedics, Chinese PLA General Hospital, Beijing 100853, P.R. China.
authors.forename: Q I
authors.initials: QI
authors.lastname: Guo
authors.affiliation: Department of Orthopedics, The 309 Hospital of PLA, Beijing 100091, P.R. China.
authors.forename: Daohong
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, Chinese PLA General Hospital, Beijing 100853, P.R. China.
authors.forename: Peifu
authors.initials: P
authors.lastname: Tang
keywords: acute wound; bacterial contamination; biofilm prevention; early application; negative pressure wound therapy