title: Biosurfactant production through Bacillus sp. MTCC 5877 and its multifarious applications in food industry.
abstract: In this study Bacillus sp. MTCC5877 was explored for the production of biosurfactant (BSs) and various carbon sources 1% (w/v), 0.5% (w/v) nitrogen sources were tested at different pH, and temperature. Yield was measured in terms of Emulsification index (EI), Oil Displacement Area (ODA) and Drop Collapse Area (DCA) and maximum emulsification activities of BSs were found (E24) 50%, 76% and 46%, respectively, and maximum ODA of 5.0, 6.2 and 4.7cm, were shown respectively. The BS was able to reduce the surface tension of water from 72 to 30mN/m and 72 to 32mN/m. Structural compositions of BS were confirmed by FTIR, GC-MS and NMR. Anti-adhesive property of BS was determined and found effective against biofilm formation. It could remove 73% Cd from vegetable which confirms its application in food industry.
authors.affiliation: Department of Biotechnology, Motilal Nehru National Institute of Technology Allahabad, Allahabad 211004, U.P., India.
authors.forename: Farhan
authors.initials: F
authors.lastname: Anjum
authors.affiliation: Department of Biotechnology, Motilal Nehru National Institute of Technology Allahabad, Allahabad 211004, U.P., India.
authors.forename: Gunjan
authors.initials: G
authors.lastname: Gautam
authors.affiliation: Bioenergy and Energy Planning Research Group, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland.
authors.forename: Gnansounou
authors.initials: G
authors.lastname: Edgard
authors.affiliation: Department of Biotechnology, Motilal Nehru National Institute of Technology Allahabad, Allahabad 211004, U.P., India. Electronic address: sn5@mnnit.ac.in.
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Negi
mesh_terms: D001407:Bacillus / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D002104:Cadmium / Q000302:isolation & purification; D002244:Carbon / Q000378:metabolism; D019649:Food Industry / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D009584:Nitrogen / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013500:Surface Tension; D013501:Surface-Active Agents / Q000737:chemistry* / Q000378:metabolism*; D013696:Temperature
chemical_list: D013501:Surface-Active Agents; D002104:Cadmium; D002244:Carbon; D009584:Nitrogen
keywords: Biosurfactant; Emulsification activity; GC–MS; Heavy metal removal; Oil Displacement Area