title: Simultaneous flow cytometric measurement of antigen attachment to phagocytes and phagocytosis.
abstract: The current available assays cannot differentiate the stages of phagocytosis. We, therefore, established methods for concurrent detection of antigen attachment and engulfment by phagocyte using latex beads coated with lipopolysaccharide, rabbit IgG, and carboxyfluorescein diacetate succinimidyl ester. The generated beads were incubated with whole blood at 37°C for 1 hr and stained with PE-Cy5.5 anti-rabbit IgG antibody. By flow cytometry, attachment and phagocytic processes could be detected, simultaneously. The established method is a valuable tool for diagnosis of phagocytic disorder and study of molecules involved in phagocytosis.
authors.affiliation: a Division of Clinical Immunology, Department of Medical Technology , Chiang Mai University , Chiang Mai , Thailand.
authors.forename: Witida
authors.initials: W
authors.lastname: Laopajon
authors.affiliation: a Division of Clinical Immunology, Department of Medical Technology , Chiang Mai University , Chiang Mai , Thailand.
authors.forename: Nuchjira
authors.initials: N
authors.lastname: Takheaw
authors.affiliation: a Division of Clinical Immunology, Department of Medical Technology , Chiang Mai University , Chiang Mai , Thailand.|b Biomedical Technology Research Center, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency at the Faculty of Associated Medical Sciences , Chiang Mai University , Chiang Mai , Thailand.
authors.forename: Watchara
authors.initials: W
authors.lastname: Kasinrerk
authors.affiliation: a Division of Clinical Immunology, Department of Medical Technology , Chiang Mai University , Chiang Mai , Thailand.|b Biomedical Technology Research Center, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency at the Faculty of Associated Medical Sciences , Chiang Mai University , Chiang Mai , Thailand.
authors.forename: Supansa
authors.initials: S
authors.lastname: Pata
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood / Q000276:immunology; D000941:Antigens / Q000032:analysis* / Q000097:blood / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology; D008863:Microspheres; D010586:Phagocytes / Q000166:cytology* / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology*; D011817:Rabbits
chemical_list: D000906:Antibodies; D000941:Antigens; D007074:Immunoglobulin G; D008070:Lipopolysaccharides
keywords: attachment; engulfment; flow cytometry; opsonized beads; phagocytes; phagocytosis