title: Human TUBG2 gene is expressed as two splice variant mRNA and involved in cell growth.
abstract: In mammals, γ-tubulin, a key protein in microtubule nucleation, is encoded by two genes, TUBG1 and TUBG2. Human TUBG1 and TUBG2 mRNA are expressed ubiquitously and predominantly in preimplantation embryos and the brain, respectively, but specific detection of γ-tubulin2 protein expression is difficult due to their high sequence similarity. Here, we describe a protocol for differential detection of two human γ-tubulins by western blotting. In several cancer cell lines and the brain, expression of γ-tubulin2 along with γ-tubulin1 and a novel TUBG2 splice variant are identified. Contribution of ectopically expressed γ-tubulin2 in cancer growth was determined by depletion of γ-tubulin2.
authors.affiliation: Department of Life Sciences Graduate School of Arts and Sciences, The University of Tokyo, Japan.
authors.forename: Tsubasa
authors.initials: T
authors.lastname: Ohashi
authors.affiliation: Cell Signal Unit, Okinawa Institute of Science and Technology, Japan.|RIKEN Center for Integrative Medical Sciences, Yokohama, Kanagawa, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yamamoto
authors.affiliation: Division of Genetics, Department of Cancer Biology, Institute of Medical Science, University of Tokyo, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yamanashi
authors.affiliation: Department of Life Sciences Graduate School of Arts and Sciences, The University of Tokyo, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Ohsugi
mesh_terms: D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D012326:RNA Splicing / Q000235:genetics*; D034741:RNA, Small Interfering / Q000378:metabolism; D014162:Transfection; D014404:Tubulin / Q000235:genetics* / Q000378:metabolism
chemical_list: D034741:RNA, Small Interfering; C000619121:TUBG2 protein, human; D014404:Tubulin
keywords: cancer; centrosome; γ-tubulin