title: Transcriptome analyses of insect cells to facilitate baculovirus-insect expression.
abstract: The High Five cell line (BTI-TN-5B1-4) isolated from the cabbage looper, Trichoplusia ni is an insect cell line widely used for baculovirus-mediated recombinant protein expression. Despite its widespread application in industry and academic laboratories, the genomic background of this cell line remains unclear. Here we sequenced the transcriptome of High Five cells and assembled 25,234 transcripts. Codon usage analysis showed that High Five cells have a robust codon usage capacity and therefore suit for expressing proteins of both eukaryotic- and prokaryotic-origin. Genes involved in glycosylation were profiled in our study, providing guidance for engineering glycosylated proteins in the insect cells. We also predicted signal peptides for transcripts with high expression abundance in both High Five and Sf21 cell lines, and these results have important implications for optimizing the expression level of some secretory and membrane proteins.
authors.affiliation: Biodynamic Optical Imaging Center, School of Life Science, Peking University, Beijing, 100871, China.|State Key Laboratory of Protein and Plant Gene Research, Peking University, Beijing, 100871, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yu
authors.affiliation: Biodynamic Optical Imaging Center, School of Life Science, Peking University, Beijing, 100871, China.|State Key Laboratory of Protein and Plant Gene Research, Peking University, Beijing, 100871, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Biodynamic Optical Imaging Center, School of Life Science, Peking University, Beijing, 100871, China.|State Key Laboratory of Protein and Plant Gene Research, Peking University, Beijing, 100871, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Tang
authors.affiliation: Biodynamic Optical Imaging Center, School of Life Science, Peking University, Beijing, 100871, China.|State Key Laboratory of Protein and Plant Gene Research, Peking University, Beijing, 100871, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, Peking University, Beijing, 100871, China. junyuxiao@pku.edu.cn.|Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China. junyuxiao@pku.edu.cn.
authors.forename: Junyu
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Biodynamic Optical Imaging Center, School of Life Science, Peking University, Beijing, 100871, China. xdsu@pku.edu.cn.|State Key Laboratory of Protein and Plant Gene Research, Peking University, Beijing, 100871, China. xdsu@pku.edu.cn.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Su
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D003062:Codon; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006031:Glycosylation; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D061987:Sf9 Cells; D018411:Spodoptera / Q000235:genetics*
chemical_list: D003062:Codon; D021382:Protein Sorting Signals; D011994:Recombinant Proteins
keywords: High Five cell line; baculovirus-insect cell system; codon usage; glycosylation; signal peptide