title: Flavonoids, Antioxidant Potential, and Acetylcholinesterase Inhibition Activity of the Extracts from the Gametophyte and Archegoniophore of Marchantia polymorpha L.
abstract: Marchantia polymorpha L. is a representative bryophyte used as a traditional Chinese medicinal herb for scald and pneumonia. The phytochemicals in M. polymorpha L. are terpenoids and flavonoids, among which especially the flavonoids show significant human health benefits. Many researches on the gametophyte of M. polymorpha L. have been reported. However, as the reproductive organ of M. polymorpha L., the bioactivity and flavonoids profile of the archegoniophore have not been reported, so in this work the flavonoid profiles, antioxidant and acetylcholinesterase inhibition activities of the extracts from the archegoniophore and gametophyte of M. polymorpha L. were compared by radical scavenging assay methods (DPPH, ABTS, O(2-)), reducing power assay, acetylcholinesterase inhibition assay and LC-MS analysis. The results showed that the total flavonoids content in the archegoniophore was about 10-time higher than that of the gametophyte. Differences between the archegoniophore and gametophyte of M. polymorpha L. were observed by LC-MS analysis. The archegoniophore extracts showed stronger bio-activities than those of the gametophyte. The archegoniophore extract showed a significant acetylcholinesterase inhibition, while the gametophyte extract hardly inhibited it.
authors.affiliation: Insititute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China. xinwang2015@yahoo.com.|University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China. xinwang2015@yahoo.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China. cao101@shnu.edu.cn.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Cao
authors.affiliation: Insititute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China. wangqx@shnu.edu.cn.|College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China. wangqx@shnu.edu.cn.
authors.forename: Yuhuan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China. yuhuanwu@iae.ac.cn.|Shanghai Key Laboratory of Plant Functional Genomics and Resources, Chinese Academy of Sciences, Shanghai Chenshan Botanical Garden, Shanghai 200234, China. yuhuanwu@iae.ac.cn.
authors.forename: Quanxi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau. jianboxiao@yahoo.com.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Xiao
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D002853:Chromatography, Liquid; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D055993:Germ Cells, Plant / Q000737:chemistry*; D006801:Humans; D020128:Inhibitory Concentration 50; D047008:Marchantia / Q000737:chemistry*; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D002800:Cholinesterase Inhibitors; D005419:Flavonoids; D016166:Free Radical Scavengers; D010936:Plant Extracts; D000110:Acetylcholinesterase
keywords: Marchantia polymorpha L.; acetylcholinesterase inhibition; antioxidant potential; archegoniophore; flavonoids; gametophyte