title: Decoration of heparin and bovine serum albumin on polysulfone membrane assisted via polydopamine strategy for hemodialysis.
abstract: Renal failure brings about abnormality of waste and toxins and deposition in the body. In clinic, the waste and toxins in vitro are eliminated by hemodialysis device with polysulfone (PSF) porous membranes. In the work, decoration of heparin (Hep) and bovine serum albumin (BSA) on PSF membranes would be beneficial to improve the hemocompatibility and reduce the anaphylatoxin formation during hemodialysis. The PSF porous membranes are surface-modified by simply dipping them into dopamine aqueous solution for 8 h. Then, Hep and BSA are immobilized covalently onto the resultant membrane. Attenuated total reflectance Fourier transform infrared spectra (ATR-FTIR) confirms that Hep and BSA are successfully introduced onto the surface of PSF membranes. Scanning electronic microscopy (SEM) and atomic force microscopy (AFM) display the changes of surface morphologies after modification. The result of water contact angle measurement shows that the hydrophilicity of PSF membranes is remarkably improved after coating polydopamine (pDA) and binding Hep and BSA. The experiments of hemocompatibility indicate that Hep and BSA grafted onto membranes suppress the adhesion of platelet and enhance the anticoagulation ability of PSF membranes. Furthermore, the protein adsorption tests reveal that Hep and BSA immobilized onto membranes depress the protein absorption and develop antifouling-protein ability of pristine membrane. This study proves a convenient and simple approach to graft two functional organic polymers which, respectively, play a vital role and then improve the hemocompatibility and biocompatibility of PSF membranes for their biomedical and blood-contacting applications.
authors.affiliation: a Department of Orthodontics, College of Stomatology , Chongqing Medical University , Chongqing , China.|b Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences , Chongqing Medical University , Chongqing , China.
authors.forename: Bingwu
authors.initials: B
authors.lastname: Xie
authors.affiliation: c Department of Stomatology, Beijing Anzhen Hospital , Capital Medical University , Beijing , China.
authors.forename: Ranran
authors.initials: R
authors.lastname: Zhang
authors.affiliation: d Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies , Peking University , Beijing , China.
authors.forename: Huan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: a Department of Orthodontics, College of Stomatology , Chongqing Medical University , Chongqing , China.|b Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences , Chongqing Medical University , Chongqing , China.
authors.forename: Anxiu
authors.initials: A
authors.lastname: Xu
authors.affiliation: d Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies , Peking University , Beijing , China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Deng
authors.affiliation: c Department of Stomatology, Beijing Anzhen Hospital , Capital Medical University , Beijing , China.
authors.forename: Yalin
authors.initials: Y
authors.lastname: Lv
authors.affiliation: a Department of Orthodontics, College of Stomatology , Chongqing Medical University , Chongqing , China.|b Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences , Chongqing Medical University , Chongqing , China.
authors.forename: Feng
authors.initials: F
authors.lastname: Deng
authors.affiliation: b Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences , Chongqing Medical University , Chongqing , China.|d Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies , Peking University , Beijing , China.|e Laboratory of Interdisciplinary Studies, Department of Oral and Maxillofacial Surgery , Peking University School and Hospital of Stomatology , Beijing , China.
authors.forename: Shicheng
authors.initials: S
authors.lastname: Wei
mesh_terms: D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects; D002417:Cattle; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D007211:Indoles / Q000737:chemistry*; D008422:Materials Testing; D008567:Membranes, Artificial; D010973:Platelet Adhesiveness / Q000187:drug effects; D011108:Polymers / Q000737:chemistry*; D006435:Renal Dialysis / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012844:Sincalide / Q000378:metabolism; D013450:Sulfones / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D007211:Indoles; D008567:Membranes, Artificial; D011108:Polymers; D013450:Sulfones; C568283:polydopamine; D014867:Water; C017662:polysulfone P 1700; D012710:Serum Albumin, Bovine; D006493:Heparin; D012844:Sincalide
keywords: Polydopamine; bovine serum albumin; hemodialysis; heparin; polysulfone