title: Response of enzyme activities and microbial communities to soil amendment with sugar alcohols.
abstract: Changes in microbial community structure are widely known to occur after soil amendment with low-molecular-weight organic compounds; however, there is little information on concurrent changes in soil microbial functional diversity and enzyme activities, especially following sorbitol and mannitol amendment. Soil microbial functional diversity and enzyme activities can be impacted by sorbitol and mannitol, which in turn can alter soil fertility and quality. The objective of this study was to investigate the effects of sorbitol and mannitol addition on microbial functional diversity and enzyme activities. The results demonstrated that sorbitol and mannitol addition altered the soil microbial community structure and improved enzyme activities. Specifically, the addition of sorbitol enhanced the community-level physiological profile (CLPP) compared with the control, whereas the CLPP was significantly inhibited by the addition of mannitol. The results of a varimax rotated component matrix demonstrated that carbohydrates, polymers, and carboxylic acids affected the soil microbial functional structure. Additionally, we found that enzyme activities were affected by both the concentration and type of inputs. In the presence of high concentrations of sorbitol, the urease, catalase, alkaline phosphatase, β-glucosidase, and N-acetyl-β-d-glucosaminidase activities were significantly increased, while invertase activity was decreased. Similarly, this increase in invertase, catalase, and alkaline phosphatase and N-acetyl-β-d-glucosaminidase activities was especially evident after mannitol addition, and urease activity was only slightly affected. In contrast, β-glucosidase activity was suppressed at the highest concentration. These results indicate that microbial community diversity and enzyme activities are significantly affected by soil amendment with sorbitol and mannitol.
authors.affiliation: Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, Henan, 450000, China.
authors.forename: Huili
authors.initials: H
authors.lastname: Yu
authors.affiliation: Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, Henan, 450000, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Si
authors.affiliation: Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, Henan, 450000, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shao
authors.affiliation: Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, Henan, 450000, China.
authors.forename: Xiansheng
authors.initials: X
authors.lastname: Qiao
authors.affiliation: Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, Henan, 450000, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Yang
authors.affiliation: Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, Henan, 450000, China.
authors.forename: Dengtao
authors.initials: D
authors.lastname: Gao
authors.affiliation: Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, Henan, 450000, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D001419:Bacteria / Q000201:enzymology*; D044822:Biodiversity; D018533:Biomass; D002374:Catalase / Q000378:metabolism; D008353:Mannitol / Q000494:pharmacology*; D064307:Microbiota / Q000187:drug effects*; D025341:Principal Component Analysis; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000737:chemistry; D013012:Sorbitol / Q000494:pharmacology*; D013402:Sugar Alcohols / Q000494:pharmacology*; D014510:Urease / Q000378:metabolism; D043324:beta-Fructofuranosidase / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D012987:Soil; D012989:Soil Pollutants; D013402:Sugar Alcohols; D008353:Mannitol; D013012:Sorbitol; D002374:Catalase; D000469:Alkaline Phosphatase; D001617:beta-Glucosidase; D043324:beta-Fructofuranosidase; D000118:Acetylglucosaminidase; D014510:Urease
keywords: Biolog CLPP; enzyme activity; soil microbial community; sugar alcohols