title: Detection and Characteristics of Rifampicin-Resistant Isolates of Mycobacterium tuberculosis.
abstract: Genotyping and analysis the drug resistance of 59 isolates of M. tuberculosis obtained from patients living in Altai Territory were performed using a BACTEC MGIT 960 fluorometric system by means of VNTR typing (variable number tandem repeat), PCR-RFLP analysis, and sequence analysis. The occurrence frequency was highest for isolates of the Beijing family (n=30, 50.8%). Analysis of mutation spectrum in the rpoB gene associated with rifampicin resistance revealed the major mutation (codon 531 of the rpoB gene) in 93% samples, which allows us to use rapid test systems.
authors.affiliation: Research Institute of Tuberculosis, Ministry of Health of the Russian Federation, Novosibirsk, Russia.
authors.forename: A G
authors.initials: AG
authors.lastname: Cherednichenko
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia. maya.a.rot@gmail.com.|Novosibirsk State University, Novosibirsk, Russia. maya.a.rot@gmail.com.
authors.forename: M A
authors.initials: MA
authors.lastname: Dymova
authors.affiliation: Altai Regional Antituberculous Dispensary, Barnaul, Russia.
authors.forename: O A
authors.initials: OA
authors.lastname: Solodilova
authors.affiliation: Research Institute of Tuberculosis, Ministry of Health of the Russian Federation, Novosibirsk, Russia.
authors.forename: T I
authors.initials: TI
authors.lastname: Petrenko
authors.affiliation: Altai Regional Antituberculous Dispensary, Barnaul, Russia.
authors.forename: A I
authors.initials: AI
authors.lastname: Prozorov
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.|Novosibirsk State University, Novosibirsk, Russia.
authors.forename: M L
authors.initials: ML
authors.lastname: Filipenko
mesh_terms: D000328:Adult; D000995:Antitubercular Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D018598:Minisatellite Repeats / Q000235:genetics; D058889:Molecular Typing; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D012150:Polymorphism, Restriction Fragment Length; D012293:Rifampin / Q000494:pharmacology*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D055815:Young Adult
chemical_list: D000995:Antitubercular Agents; D001426:Bacterial Proteins; C505805:rpoB protein, Mycobacterium tuberculosis; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin
keywords: Mycobacterium tuberculosis; drug resistance; rifampicin; rpoB gene