title: Cathelicidin-BF ameliorates lipopolysaccharide-induced intestinal epithelial barrier disruption in rat.
abstract: OBJECTIVE
  The present study examined the effect of the antimicrobial peptide cathelicidin-BF (CBF) on LPS-induced mucosal injury and intestinal epithelial barrier dysfunction in a rat model and in the porcine intestinal epithelial cell line.
  
  
  METHODS
  Changes in barrier integrity were assessed in intestinal epithelium and IPEC-J2 monolayers by measuring nutrient absorption and transepithelial electrical resistance (TER), and the permeability of intestinal epithelium was examined by measuring plasma d-lactate and diamine oxidase levels. The expression levels of tight junction (TJ) proteins were quantified by real-time PCR, and immunofluorescence was used to analyse the location and distribution of TJs in cells.
  
  
  RESULTS
  In vivo, CBF improved epithelial barrier function through attenuating the alterations of the mucosal structure, nutrient absorption and TER in the jejunum, and preventing the down-regulation of TJ proteins in LPS-induced rat intestinal epithelium. In vitro, CBF prevented the disruption and the re-distribution of ZO-1 and occludin, and suppressed the increase in inflammatory cytokine levels in LPS-induced IPEC-J2. The CBF-induced upregulation of zonula occludens-1 and occludin was prevented by U0126 or SB203580, suggesting the involvement of the MEK and p38 MAPK pathways in the CBF-induced changes in tight junctions.
  
  
  CONCLUSIONS
  Our results showed that CBF prevents LPS-induced intestinal epithelial barrier dysfunction, suggesting its potential as a therapeutic agent for the prevention of LPS-mediated intestinal diseases. We found that exogenous CBF had protective effects on LPS-induced intestinal epithelial barrier disruption in rats and on epithelial damage in IPEC-J2 cells.
authors.affiliation: Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Han
authors.affiliation: Key Laboratory of Animal Nutrition and Feed Science (Hua Dong), Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Zeqing
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Key Laboratory of Animal Nutrition and Feed Science (Hua Dong), Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Animal Nutrition and Feed Science (Hua Dong), Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Xia
authors.affiliation: Key Laboratory of Animal Nutrition and Feed Science (Hua Dong), Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Haiwen
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Animal Nutrition and Feed Science (Hua Dong), Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xinxia
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Nutrition and Feed Science (Hua Dong), Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: yzwang@zju.edu.cn.
authors.forename: Yizhen
authors.initials: Y
authors.lastname: Wang
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000627:therapeutic use*; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D017097:Electric Impedance; D007408:Intestinal Absorption / Q000187:drug effects; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism; D019344:Lactic Acid / Q000097:blood; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000633:toxicity*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013552:Swine; D062725:Tight Junction Proteins / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects; D062826:Zonula Occludens-1 Protein / Q000096:biosynthesis; D054804:Cathelicidins
chemical_list: D023181:Antimicrobial Cationic Peptides; D016207:Cytokines; D008070:Lipopolysaccharides; D062725:Tight Junction Proteins; D062826:Zonula Occludens-1 Protein; D019344:Lactic Acid; D006631:Amine Oxidase (Copper-Containing); D054804:Cathelicidins
keywords: Cathelicidin-BF; Intestinal epithelial barrier disruption; Lipopolysaccharide; Tight junction