title: Coupling a DNA-ligand ensemble with Ag cluster formation for the label-free and ratiometric detection of intracellular biothiols.
abstract: We have demonstrated the synthesis of a smart nanoprobe by coupling a DNA-ligand ensemble with Ag cluster formation that allows the label-free and ratiometric detection of intracellular biothiols with high sensitivity and selectivity as well as discrimination of normal cells and cancer cells.
authors.affiliation: Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China. jren@ciac.ac.cn xqu@ciac.ac.cn.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Cao
authors.forename: Enguo
authors.initials: E
authors.lastname: Ju
authors.forename: Chaoqun
authors.initials: C
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: Pu
authors.forename: Jinsong
authors.initials: J
authors.lastname: Ren
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Qu
mesh_terms: D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005978:Glutathione / Q000032:analysis*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008024:Ligands; D053768:Metal Nanoparticles / Q000737:chemistry*; D018613:Microscopy, Confocal; D009369:Neoplasms / Q000175:diagnosis; D061848:Optical Imaging; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D008024:Ligands; D013438:Sulfhydryl Compounds; D012834:Silver; D004247:DNA; D005978:Glutathione