title: Rapid and Sensitive Determination of the Major Steroidal Saponins of Ypsilandra thibetica Franch by Ultra High-Performance Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry.
abstract: A rapid and validated method using ultra high-performance liquid chromatography coupled with a triple quadrupole mass spectrometry (UHPLC-QQQ MS) was developed for simultaneous determination of four active steroidal saponins, i.e., dichotomin ( 1: ), pennogenin 3-O-α-l-arabinofuranosyl-(1→4)-[α-l-rhamnopyranosyl-(1→2)]-β-d-glucopyranoside ( 2: ), pennogenin 3-O-α-l-rhamnopyranosyl-(1→2)-[α-l-rhamnopyranosyl-(1→4)-α-l-rhamnopyranosyl-(1→4)]-β-d-glucopyranoside ( 3: ) and diosgenin 3-O-α-l-rhamnopyranosyl-(1→2)-[α-l-rhamnopyranosyl-(1→4)-α-l-rhamnopyranosyl-(1→4)]-β-d-glucopyranosidein ( 4: ), in Ypsilandra thibetica Franch. The optimized sample preparation and UHPLC-QQQ MS conditions were chosen for quantitative analysis. The separation was performed on an Agilent Zorbax Eclipse Plus C18 column (2.1 mm × 50 mm, 1.8 µm) with gradient elution of acetonitrile-0.1% formic acid in water. All calibration curves showed good linear regression (r> 0.9985) within the test range. The limits of detection and quantification were in the range of 0.02-4.40 and 0.04-22.0 ng/mL, respectively. The proposed method was applied to analyze two batches of Y. thibetica samples for target compounds within 10 min. This work promoted the quality control method for raw material or preparations of Y. thibetica.
authors.affiliation: School of Traditional Chinese Medicine, Guangdong Food and Drug Vocational College, Guangzhou 510520, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Xia
authors.affiliation: School of Traditional Chinese Medicine, Guangdong Food and Drug Vocational College, Guangzhou 510520, PR China.
authors.forename: Pu-Yue
authors.initials: PY
authors.lastname: Ouyang
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Wen
authors.initials: W
authors.lastname: Gao
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Kowloon, Hong Kong Special Administrative Region, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yi
authors.affiliation: School of Traditional Chinese Medicine, Guangdong Food and Drug Vocational College, Guangzhou 510520, PR China.
authors.forename: Xian-Tao
authors.initials: XT
authors.lastname: Zhang
authors.affiliation: School of Traditional Chinese Medicine, Guangdong Food and Drug Vocational College, Guangzhou 510520, PR China.
authors.forename: Zhen-Dong
authors.initials: ZD
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China yanghuacpu@126.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Yang
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D057230:Limit of Detection; D013058:Mass Spectrometry; D000070556:Melanthiaceae / Q000737:chemistry*; D012503:Saponins / Q000032:analysis*; D013995:Time
chemical_list: D012503:Saponins