title: Plasma from pre-eclamptic patients induces the expression of the anti-angiogenic miR-195-5p in endothelial cells.
abstract: We examined the effect of plasma incubation from preeclampsia pregnant on the antiangiogenic miR-195-5p expression. Higher miR-195-5p expression was found in cultures incubated with preeclampsia plasma compared to those incubated with healthy pregnant plasma. Next, as VEGF is a target of miR-195-5p we have quantified its expression by real-time qPCR and ELISA. We found reduced VEGF levels in culture incubated with preeclampsia plasma. Therefore, we have concluded that the higher expression of miR-195-5p in endothelial cell cultures incubated with preeclampsia plasma may contribute to decreased expression of VEGFA (gene and protein) and increased antiangiogenic status in preeclampsia. Therefore, this miR may be an important target in preeclampsia.
authors.affiliation: Department of Pharmacology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brazil.
authors.forename: Valeria Cristina
authors.initials: VC
authors.lastname: Sandrim
authors.affiliation: Department of Pharmacology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brazil.
authors.forename: Mayara Caldeira
authors.initials: MC
authors.lastname: Dias
authors.affiliation: Blood Transfusion Center, Cell Engineering Laboratory, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, SP, Brazil.
authors.forename: Ana Lívia de Carvalho
authors.initials: AL
authors.lastname: Bovolato
authors.affiliation: Department of Pharmacology, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil.
authors.forename: Jose E
authors.initials: JE
authors.lastname: Tanus-Santos
authors.affiliation: Blood Transfusion Center, Cell Engineering Laboratory, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, SP, Brazil.
authors.forename: Elenice
authors.initials: E
authors.lastname: Deffune
authors.affiliation: Department of Gynecology and Obstetrics, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil.
authors.forename: Ricardo C
authors.initials: RC
authors.lastname: Cavalli
mesh_terms: D000328:Adult; D020533:Angiogenesis Inhibitors / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D018919:Neovascularization, Physiologic; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D020533:Angiogenesis Inhibitors; C552722:MIRN195 microRNA, human; D035683:MicroRNAs; D042461:Vascular Endothelial Growth Factor A
keywords: HUVECs; VEGF; angiogenesis; miR-195; microRNA; pre-eclampsia