title: Hermes (Rbpms) is a Critical Component of RNP Complexes that Sequester Germline RNAs during Oogenesis.
abstract: The germ cell lineage inXenopusis specified by the inheritance of germ plasm that assembles within the mitochondrial cloud or Balbiani body in stage I oocytes. Specific RNAs, such asnanos1, localize to the germ plasm.nanos1has the essential germline function of blocking somatic gene expression and thus preventing Primordial Germ Cell (PGC) loss and sterility. Hermes/Rbpms protein andnanosRNA co-localize within germinal granules, diagnostic electron dense particles found within the germ plasm. Previous work indicates thatnanosaccumulates within the germ plasm through a diffusion/entrapment mechanism. Here we show that Hermes/Rbpms interacts withnanosthrough sequence specific RNA localization signals found in thenanos-3'UTR. Importantly, Hermes/Rbpms specifically bindsnanos, but notVg1RNA in the nucleus of stage I oocytes.In vitrobinding data show that Hermes/Rbpms requires additional factors that are present in stage I oocytes in order to bindnanos1. One such factor may be hnRNP I, identified in a yeast-2-hybrid screen as directly interacting with Hermes/Rbpms. We suggest that Hermes/Rbpms functions as part of a RNP complex in the nucleus that facilitates selection of germline RNAs for germ plasm localization. We propose that Hermes/Rbpms is required fornanosRNA to form within the germinal granules and in this way, participates in the germline specific translational repression and sequestration ofnanos RNA.
authors.affiliation: Department of Cell Biology, University of Miami School of Medicine, Miami, FL 33136, USA.
authors.forename: Tristan
authors.initials: T
authors.lastname: Aguero
authors.affiliation: Department of Cell Biology, University of Miami School of Medicine, Miami, FL 33136, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Houston Methodist Research Institute, The Houston Methodist Hospital, Bertner Avenue, Houston, TX 77030, USA.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Kloc
authors.affiliation: Laboratoire de Biologie du Développement de Villefranche-sur-mer (LBDV), Sorbonne Universités, UPMC Univ Paris 06, CNRS, Villefranche-sur-mer 06230, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Chang
authors.affiliation: Laboratoire de Biologie du Développement de Villefranche-sur-mer (LBDV), Sorbonne Universités, UPMC Univ Paris 06, CNRS, Villefranche-sur-mer 06230, France.
authors.forename: Evelyn
authors.initials: E
authors.lastname: Houliston
authors.affiliation: Department of Cell Biology, University of Miami School of Medicine, Miami, FL 33136, USA.
authors.forename: Mary Lou
authors.initials: ML
authors.lastname: King
keywords: RNP particle; Xenopus oogenesis; germinal granules; germline; nanos ribonucleoprotein complex; nanos1 RNA localization