title: Anti-obesity potential of enzymatic fragments of hyaluronan on high-fat diet-induced obesity in C57BL/6 mice.
abstract: Hyaluronan has diverse biological activities depending on its molecular size. The hyaluronan fragments (50 kDa) can decrease adipogenic differentiation in vitro. However, in vivo anti-obesitic effects of hyaluronan fragments have not been elucidated. Therefore, we examined the anti-obesity effects of hyaluronan fragments on high-fat diet induced obesity in C57BL/6 mice. Oral administration of hyaluronan fragments (200 mg/kg for 8 weeks) decreased body weight, adipose tissues, serum lipid (low-density lipoprotein cholesterol, triglyceride), and leptin level. Hyaluronan fragments decreased the hypertrophy of adipose tissue and ameliorated liver steatosis. The mRNA expression of leptin was reduced in adipocyte by treatment with hyaluronan fragments. Additionally, hyaluronan fragments enhanced the mRNA expression of PPAR-α and its target genes UCP-2 and decreased mRNA expression of PPAR- γ and fatty acid synthase in liver. In conclusions, hyaluronan fragments had marked effects on inhibiting the development of obesity in obese mice fed the high-fat diet. It suggested that enhancing PPAR-α and suppressing PPAR-γ expression are two possible mechanisms for the anti-obesitic effect of hyaluronan fragments.
authors.affiliation: Department of Physiology, Catholic Kwandong University College of Medicine, Gangneung, 25601, Republic of Korea. Electronic address: bgpark@cku.ac.kr.
authors.forename: Byong-Gon
authors.initials: BG
authors.lastname: Park
authors.affiliation: Department of Microbiology, Catholic Kwandong University College of Medicine, Gangneung, 25601, Republic of Korea. Electronic address: pyskth@cku.ac.kr.
authors.forename: Yoon-Sun
authors.initials: YS
authors.lastname: Park
authors.affiliation: Biostream Technologies, Yongin-si, Gyeonggi-do, 17098, Republic of Korea. Electronic address: pjwrnds@empas.com.
authors.forename: Joo Woong
authors.initials: JW
authors.lastname: Park
authors.affiliation: Biostream Technologies, Yongin-si, Gyeonggi-do, 17098, Republic of Korea. Electronic address: eunji.shin0930@gmail.com.
authors.forename: Eunji
authors.initials: E
authors.lastname: Shin
authors.affiliation: Department of Microbiology, Catholic Kwandong University College of Medicine, Gangneung, 25601, Republic of Korea. Electronic address: shinws@cku.ac.kr.
authors.forename: Woon-Seob
authors.initials: WS
authors.lastname: Shin
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D052242:Adiponectin / Q000097:blood; D000818:Animals; D001835:Body Weight; D059305:Diet, High-Fat / Q000009:adverse effects*; D005234:Fatty Liver / Q000473:pathology; D006820:Hyaluronic Acid / Q000494:pharmacology*; D006949:Hyperlipidemias / Q000378:metabolism; D020738:Leptin / Q000097:blood / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008099:Liver / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D008970:Molecular Weight; D009765:Obesity / Q000503:physiopathology / Q000628:therapy*; D047493:PPAR alpha / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D014280:Triglycerides / Q000097:blood
chemical_list: D052242:Adiponectin; D020738:Leptin; D008055:Lipids; D008077:Lipoproteins, LDL; D047493:PPAR alpha; D014280:Triglycerides; D006820:Hyaluronic Acid
keywords: Antiobesity; High-fat diet; Hyaluronan fragments; Liver steatosis; PPAR-Alpha