title: [The protective effect of bone marrow mesenchymal stem cells carrying antioxidant gene superoxide dismutase on paraquat lung injury in mice].
abstract: OBJECTIVE
  To explore the possible mechanism and protective effect of BMSCs (bone mesenchymal stem cells) carrying superoxide dismutase (SOD) gene on mice with paraquat-induced acute lung injury.
  
  
  METHODS
  To establish the cell line of BMSCs bringing SOD gene, lentiviral vector bringing SOD gene was built and co-cultured with BMSCs. A total of 100 BALB/c mice were randomly divided into five groups, namely Control group, poisoning group (PQ group) , BMSCs therapy group (BMSC group) , BMSCs-Cherry therapy group (BMSC-Cherry group) , BMSCs-SOD therapy group (BMSC-SOD group) . PQ poisoning model was produced by stomach lavaged once with 1 ml of 25 mg/kg PQ solution, and the equal volume of normal saline (NS) was given to Control group mice instead of PQ. The corresponding BMSCs therapy cell lines were delivered to mice through the tail vein of mice 4h after PQ treatment.Five mice of each group were sacrificed 3 d, 7 d, 14 d and 21 days after corresponding BMSCs therapy cell lines administration, and lung tissues of mice were taken to make sections for histological analysis. The serum levels of glutathione (GSH) , malondialdehyde (MDA) , SOD, and the levels of transforming growth factor-β (TGF-β) and tumor necrosis factor-α (TNF-α) in lung tissue were determined. The level of SOD was assayed by Westen-blot.
  
  
  RESULTS
  Compared with Control group, the early (3 days) levels of SOD protein in lung tissue of PQ group obviously decreased, and the late (21 days) levels of SOD obviously increased, while in therapy groups, that was higher than that in PQ group, and the BMSCs-SOD group showed most obvious (all P<0.05) . Compared with Control group, the levels of plasma GSH and SOD of PQ group and each therapy group wae significantly lower than those in Control group, while in therapy groups, those were higher than those of PQ group, and the BMSCs-SOD group showed most obvious (all P<0.05) .Compared with Control group, the level of plasma MDA, TNF-α and TGF-β in PQ group and therapy groups were significantly higher, while in therapy groups, that was lower than that in PQ group, and the BMSCs-SOD group showed most obvious (all P<0.05) . Lung biopsy showed that, the degree of lung tissue damage in each therapy group obviously reduced.
  
  
  CONCLUSIONS
  SOD is the key factor of the removal of reactive oxygen species (ROS) in cells, that can obviously inhibit the oxidative stress damage and the apoptosis induced by PQ, thus significantly increasing alveolar epithelial cell ability to fight outside harmful environment.
authors.affiliation: Emergency Department, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Yingwei
authors.initials: Y
authors.lastname: Ding
authors.forename: Yuehui
authors.initials: Y
authors.lastname: Hou
authors.forename: Guangju
authors.initials: G
authors.lastname: Zhao
authors.forename: Yang
authors.initials: Y
authors.lastname: Lu
authors.forename: Xiao
authors.initials: X
authors.lastname: Chen
authors.forename: Qiqi
authors.initials: Q
authors.lastname: Cai
authors.forename: Guangliang
authors.initials: G
authors.lastname: Hong
authors.forename: Qiaomeng
authors.initials: Q
authors.lastname: Qiu
authors.forename: Zhongqiu
authors.initials: Z
authors.lastname: Lu
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000628:therapy*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002460:Cell Line; D005978:Glutathione / Q000097:blood; D008168:Lung / Q000473:pathology; D008315:Malondialdehyde / Q000097:blood; D045164:Mesenchymal Stem Cell Transplantation; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress; D010269:Paraquat / Q000506:poisoning*; D013482:Superoxide Dismutase / Q000097:blood / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione; D010269:Paraquat