title: Immunohistochemical expression of GLUT-1, GLUT-3, and carbonic anhydrase IX in benign odontogenic lesions.
abstract: BACKGROUND
  Some benign odontogenic lesions have a distinct biological behavior with high recurrence rates and local aggressive behavior. To determine whether glucose transporters proteins (GLUT-1 and GLUT-3) and carbonic anhydrase IX (CA IX) are associated with the development of as dentigerous cyst (DC), odontogenic keratocyst (OK), and ameloblastoma (AM), we evaluated the immunohistochemical expression of these proteins in these lesions.
  
  
  METHODS
  Immunoexpression of GLUT-1, GLUT-3, and CA IX was evaluated semiquantitative fields in each of the 20 cases of OK, AM, and DC. The cases were classified according to the scores: 0 (0% positive cells), 1 (<10% of positive cells), 2 (10-50% of positive cells), and 3 (>50% of positive cells). The statistical analysis was performed using Pearson's chi-square, Kruskal-Wallis and Mann-Whitney tests.
  
  
  RESULTS
  All cases were positive for GLUT-1 and 65% of OK showed scored 3. Staining was diffuse in 90% of OK and 85% of DC cases (P < 0.001). In 50% of OK and AM, staining was only observed in the membrane (P = 0.01). Most of the samples (66.7%) were negative for GLUT-3. Staining intensity for anhydrase was higher in the epithelium of DC when compared to OK (P = 0.01). Strong staining was observed in 55% of DC and 20% of OK samples (P = 0.01).
  
  
  CONCLUSIONS
  These results suggest that GLUT-1 may be involved in the metabolic regulation of glucose in odontogenic lesions studied. In addition, CA IX appears to influence the development of AM, OK, and DC which can explain the differences their biological behavior.
authors.affiliation: Postgraduate Program in Oral Pathology, Department of Dentistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Roseane Carvalho
authors.initials: RC
authors.lastname: Vasconcelos
authors.affiliation: Postgraduate Program in Oral Pathology, Department of Dentistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Jamile Marinho Bezerra
authors.initials: JM
authors.lastname: de Oliveira Moura
authors.affiliation: Postgraduate Program in Oral Pathology, Department of Dentistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Vilson
authors.initials: V
authors.lastname: Lacerda Brasileiro Junior
authors.affiliation: Postgraduate Program in Oral Pathology, Department of Dentistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Éricka Janine Dantas
authors.initials: ÉJ
authors.lastname: da Silveira
authors.affiliation: Postgraduate Program in Oral Pathology, Department of Dentistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil. leliabsouza@gmail.com, r.roseane@hotmail.com.
authors.forename: Lélia Batista
authors.initials: LB
authors.lastname: de Souza
mesh_terms: D000564:Ameloblastoma / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism; D000071231:Carbonic Anhydrase IX / Q000378:metabolism*; D003803:Dentigerous Cyst / Q000378:metabolism*; D051272:Glucose Transporter Type 1 / Q000378:metabolism*; D051274:Glucose Transporter Type 3 / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007573:Jaw Neoplasms / Q000378:metabolism*; D009807:Odontogenic Cysts / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; C495677:SLC2A1 protein, human; C495687:SLC2A3 protein, human; D000071231:Carbonic Anhydrase IX
keywords: carbonic anhydrase IX; glucose transporters; immunohistochemical