title: Assessment of genetic diversity in Brazilian barley using SSR markers.
abstract: Barley is a major cereal grown widely and used in several food products, beverage production and animal fodder. Genetic diversity is a key component in breeding programs. We have analyzed the genetic diversity of barley accessions using microsatellite markers. The accessions were composed of wild and domesticated barley representing genotypes from six countries and three breeding programs in Brazil. A total of 280 alleles were detected, 36 unique to Brazilian barley. The marker Bmag120 showed the greatest polymorphism information content (PIC), with the highest mean value found on chromosome three, and the lowest on chromosomes four and six. The wild accessions presented the highest diversity followed by the foreign genotypes. Genetic analysis was performed using Principal Coordinates Analysis, UPGMA clustering, and Bayesian clustering analysis implemented in Structure. All results obtained by the different methods were similar. Loss of genetic diversity has occurred in Brazilian genotypes. The number of alleles detected in genotypes released in 1980s was higher, whereas most of the cultivars released thereafter showed lower PIC and clustered in separate subgroups from the older cultivars. The use of a more diverse panel of genotypes should be considered in order to exploit novel alleles in Brazilian barley breeding programs.
authors.affiliation: Departamento de Plantas de Lavoura, Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Jéssica Rosset
authors.initials: JR
authors.lastname: Ferreira
authors.affiliation: Embrapa Trigo, Passo Fundo, RS, Brazil.
authors.forename: Jorge Fernando
authors.initials: JF
authors.lastname: Pereira
authors.affiliation: Embrapa Trigo, Passo Fundo, RS, Brazil.
authors.forename: Caroline
authors.initials: C
authors.lastname: Turchetto
authors.affiliation: Embrapa Trigo, Passo Fundo, RS, Brazil.
authors.forename: Euclydes
authors.initials: E
authors.lastname: Minella
authors.affiliation: Embrapa Trigo, Passo Fundo, RS, Brazil.
authors.forename: Luciano
authors.initials: L
authors.lastname: Consoli
authors.affiliation: Departamento de Plantas de Lavoura, Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Carla Andréa
authors.initials: CA
authors.lastname: Delatorre