title: Maintained larval growth in mussel larvae exposed to acidified under-saturated seawater.
abstract: Ocean acidification (OA) is known to affect bivalve early life-stages. We tested responses of blue mussel larvae to a wide range of pH in order to identify their tolerance threshold. Our results confirmed that decreasing seawater pH and decreasing saturation state increases larval mortality rate and the percentage of abnormally developing larvae. Virtually no larvae reared at average pHT 7.16 were able to feed or reach the D-shell stage and their development appeared to be arrested at the trochophore stage. However larvae were capable of reaching the D-shell stage under milder acidification (pHT ≈ 7.35, 7.6, 7.85) including in under-saturated seawater with Ωa as low as 0.54 ± 0.01 (mean ± s. e. m.), with a tipping point for normal development identified at pHT 7.765. Additionally growth rate of normally developing larvae was not affected by lower pHT despite potential increased energy costs associated with compensatory calcification in response to increased shell dissolution. Overall, our results on OA impacts on mussel larvae suggest an average pHT of 7.16 is beyond their physiological tolerance threshold and indicate a shift in energy allocation towards growth in some individuals revealing potential OA resilience.
authors.affiliation: Department of Biological and Environmental Sciences, University of Gothenburg, The Sven Lovén Centre for Marine Sciences, Kristineberg 566, SE-451 78 Fiskebäckskil, Sweden.
authors.forename: Alexander
authors.initials: A
authors.lastname: Ventura
authors.affiliation: Department of Biological and Environmental Sciences, University of Gothenburg, The Sven Lovén Centre for Marine Sciences, Kristineberg 566, SE-451 78 Fiskebäckskil, Sweden.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Schulz
authors.affiliation: Department of Biological and Environmental Sciences, University of Gothenburg, The Sven Lovén Centre for Marine Sciences, Kristineberg 566, SE-451 78 Fiskebäckskil, Sweden.
authors.forename: Sam
authors.initials: S
authors.lastname: Dupont
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology; D000465:Algorithms; D000704:Analysis of Variance; D000818:Animals; D002113:Calcification, Physiologic / Q000502:physiology; D002119:Calcium Carbonate / Q000378:metabolism; D002254:Carbonates / Q000378:metabolism; D005247:Feeding Behavior / Q000502:physiology; D005452:Fluoresceins / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007814:Larva / Q000254:growth & development* / Q000502:physiology; D018613:Microscopy, Confocal; D049880:Mytilus edulis / Q000254:growth & development* / Q000502:physiology; D012623:Seawater / Q000737:chemistry*
chemical_list: D002254:Carbonates; D005452:Fluoresceins; D002119:Calcium Carbonate; C007740:fluorexon