title: Rapid Flow Cytometry Test for Identification of Different Carbapenemases in Enterobacteriaceae.
abstract: A flow cytometry test was developed to identify carbapenemase production by Enterobacteriaceae and to discriminate between the different types of carbapenemases (classes A, B, and D). It is based on the detection of meropenem activity against bacteria, coupled with different carbapenemase inhibitors, which is assessed by flow cytometry. It represents a convenient, fast, and reliable approach (100% sensitivity and 100% specificity) for the detection and characterization of different carbapenemases.
authors.affiliation: Microbiology Department, Faculty of Medicine, University of Porto, Porto, Portugal Center for Research in Health Technologies and Information Systems (CINTESIS), Porto, Portugal atsilva@med.up.pt.
authors.forename: Ana P
authors.initials: AP
authors.lastname: Silva
authors.affiliation: Microbiology Department, Faculty of Medicine, University of Porto, Porto, Portugal Center for Research in Health Technologies and Information Systems (CINTESIS), Porto, Portugal.
authors.forename: Isabel
authors.initials: I
authors.lastname: Faria-Ramos
authors.affiliation: Microbiology Department, Faculty of Medicine, University of Porto, Porto, Portugal Center for Research in Health Technologies and Information Systems (CINTESIS), Porto, Portugal.
authors.forename: Elisabete
authors.initials: E
authors.lastname: Ricardo
authors.affiliation: Microbiology Department, Faculty of Medicine, University of Porto, Porto, Portugal Center for Research in Health Technologies and Information Systems (CINTESIS), Porto, Portugal.
authors.forename: Isabel M
authors.initials: IM
authors.lastname: Miranda
authors.affiliation: Microbiology Department, Faculty of Medicine, University of Porto, Porto, Portugal Center for Research in Health Technologies and Information Systems (CINTESIS), Porto, Portugal Microbiology Department, Centro Hospitalar São João, Porto, Portugal.
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Espinar
authors.affiliation: Microbiology Department, Faculty of Medicine, University of Porto, Porto, Portugal Center for Research in Health Technologies and Information Systems (CINTESIS), Porto, Portugal.
authors.forename: Sofia
authors.identifier: 0000-0003-2899-6615
authors.initials: S
authors.lastname: Costa-de-Oliveira
authors.affiliation: Servicio de Microbiología, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Cantón
authors.affiliation: Microbiology Department, Faculty of Medicine, University of Porto, Porto, Portugal Center for Research in Health Technologies and Information Systems (CINTESIS), Porto, Portugal.
authors.forename: Acácio G
authors.initials: AG
authors.lastname: Rodrigues
authors.affiliation: Microbiology Department, Faculty of Medicine, University of Porto, Porto, Portugal Center for Research in Health Technologies and Information Systems (CINTESIS), Porto, Portugal Microbiology Department, Centro Hospitalar São João, Porto, Portugal.
authors.forename: Cidália
authors.initials: C
authors.lastname: Pina-Vaz
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D001897:Boronic Acids / Q000494:pharmacology; D003023:Cloxacillin / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005434:Flow Cytometry / Q000379:methods*; D015870:Gene Expression; D006801:Humans; D000077731:Meropenem; D010406:Penicillins / Q000494:pharmacology; D013845:Thienamycins / Q000494:pharmacology*; D001618:beta-Lactamases / Q000145:classification* / Q000235:genetics / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001897:Boronic Acids; D004791:Enzyme Inhibitors; D010406:Penicillins; D013845:Thienamycins; C031367:temocillin; C028592:3-aminobenzeneboronic acid; D004492:Edetic Acid; D001618:beta-Lactamases; C063912:carbapenemase; D000077731:Meropenem; D003023:Cloxacillin