title: Long-Term Starvation and Posterior Feeding Effects on Biochemical and Physiological Responses of Midgut Gland of Cherax quadricarinatus Juveniles (Parastacidae).
abstract: We investigated the effect of long-term starvation and posterior feeding on energetic reserves, oxidative stress, digestive enzymes, and histology of C. quadricarinatus midgut gland. The crayfish (6.27 g) were randomly assigned to one of three feeding protocols: continuous feeding throughout 80 day, continuous starvation until 80 day, and continuous starvation throughout 50 day and then feeding for the following 30 days. Juveniles from each protocol were weighed, and sacrificed at day 15, 30, 50 or 80. The lipids, glycogen, reduced glutathione (GSH), soluble protein, lipid peroxidation (TBARS), protein oxidation (PO), catalase (CAT), lipase and proteinase activities, and histology were measured on midgut gland. Starved crayfish had a lower hepatosomatic index, number of molts, specific growth rate, lipids, glycogen, and GSH levels than fed animals at all assay times. The starvation did not affect the soluble protein, TBARS, PO levels and CAT. In starved juveniles the lipase activity decreased as starvation time increased, whereas proteinase activity decreased only at day 80. The histological analysis of the starved animals showed several signs of structural alterations. After 30 days of feeding, the starved-feeding animals exhibited a striking recovery of hepatosomatic index, number of molts, lipids and glycogen, GSH, lipase activity and midgut gland structure.
authors.affiliation: Biology of Reproduction and Growth in Crustaceans, Department of Biodiversity and Experimental Biology, Buenos Aires University, Buenos Aires, Argentina.|IBBEA, CONICET-UBA, Buenos Aires, Argentina.
authors.forename: Hernán Javier
authors.initials: HJ
authors.lastname: Sacristán
authors.affiliation: Ecophysiology and Ecotoxicology Laboratory, Argentinian Antarctic Institute, Buenos Aires, Argentina.
authors.forename: Martín
authors.initials: M
authors.lastname: Ansaldo
authors.affiliation: Biology of Reproduction and Growth in Crustaceans, Department of Biodiversity and Experimental Biology, Buenos Aires University, Buenos Aires, Argentina.
authors.forename: Luis Marcelo
authors.initials: LM
authors.lastname: Franco-Tadic
authors.affiliation: Physiology of Aquatic Organisms, Institute of Marine and Coastal Research, CONICET, Mar del Plata University, Mar del Plata, Argentina.
authors.forename: Analía Verónica
authors.initials: AV
authors.lastname: Fernández Gimenez
authors.affiliation: Biology of Reproduction and Growth in Crustaceans, Department of Biodiversity and Experimental Biology, Buenos Aires University, Buenos Aires, Argentina.|IBBEA, CONICET-UBA, Buenos Aires, Argentina.
authors.forename: Laura Susana
authors.initials: LS
authors.lastname: López Greco
mesh_terms: D000821:Animal Feed; D000818:Animals; D003400:Astacoidea / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D002374:Catalase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006003:Glycogen / Q000032:analysis; D007413:Intestinal Mucosa / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D008049:Lipase / Q000378:metabolism; D015227:Lipid Peroxidation; D008055:Lipids / Q000032:analysis; D018384:Oxidative Stress; D010447:Peptide Hydrolases / Q000378:metabolism; D011506:Proteins / Q000737:chemistry; D013217:Starvation
chemical_list: D008055:Lipids; D011506:Proteins; D006003:Glycogen; D002374:Catalase; D008049:Lipase; D010447:Peptide Hydrolases; D005978:Glutathione