title: Gene Knockdown in Human Rhinovirus 1B Using 2'-OMe-modified siRNAs Results in the Reactivation of the Interferon Response.
abstract: The aim of this study was to investigate the knockdown efficiency of 2'-O-methylated (2'-OMe)-modified small interfering RNAs (siRNAs) on human rhinovirus 1B (HRV1B) replication and the interferon response. Thus, 24 2'-OMe-modified siRNAs were designed to target HRV1B. The RNA levels of HRV1B, Toll-like receptor 3, melanoma differentiation-associated gene 5, retinoic acid inducible gene-I, and interferons were determined in HRV1B-infected HeLa and BEAS-2B epithelial cells transfected with 2'-OMe-modified siRNAs. The results revealed that all 2'-OMe-modified siRNAs interfered with the replication of HRV1B in a cell-specific and transfection efficiency-dependent manner. Viral activation of Toll-like receptor 3, melanoma differentiation-associated gene 5, retinoic acid inducible gene-I, and the interferon response was detected. In conclusion, the 2'-OMe-modified siRNAs used in this study could interfere with HRV1B replication, possibly leading to the reactivation of the interferon response.
authors.affiliation: Key Laboratory of Medical Virology and Viral Disease, Ministry of Health of the People's Republic of China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Guang Cheng
authors.initials: GC
authors.lastname: Xie
authors.affiliation: Key Laboratory of Medical Virology and Viral Disease, Ministry of Health of the People's Republic of China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Medical Virology and Viral Disease, Ministry of Health of the People's Republic of China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Li Li
authors.initials: LL
authors.lastname: Pang
authors.affiliation: Key Laboratory of Medical Virology and Viral Disease, Ministry of Health of the People's Republic of China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Dan Di
authors.initials: DD
authors.lastname: Li
authors.affiliation: Key Laboratory of Medical Virology and Viral Disease, Ministry of Health of the People's Republic of China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Jin
authors.affiliation: Key Laboratory of Medical Virology and Viral Disease, Ministry of Health of the People's Republic of China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Hui Ying
authors.initials: HY
authors.lastname: Li
authors.affiliation: Key Laboratory of Medical Virology and Viral Disease, Ministry of Health of the People's Republic of China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Zi Qian
authors.initials: ZQ
authors.lastname: Xu
authors.affiliation: Key Laboratory of Medical Virology and Viral Disease, Ministry of Health of the People's Republic of China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Xiang Yu
authors.initials: XY
authors.lastname: Kong
authors.affiliation: Key Laboratory of Medical Virology and Viral Disease, Ministry of Health of the People's Republic of China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Lu
authors.affiliation: Key Laboratory of Medical Virology and Viral Disease, Ministry of Health of the People's Republic of China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Zhao Jun
authors.initials: ZJ
authors.lastname: Duan
mesh_terms: D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D007372:Interferons / Q000502:physiology; D034741:RNA, Small Interfering; D012229:Rhinovirus; D014779:Virus Replication
chemical_list: D034741:RNA, Small Interfering; D007372:Interferons