title: Quercitrin treatment protects endothelial progenitor cells from oxidative damage via inducing autophagy through extracellular signal-regulated kinase.
abstract: Atherosclerosis is a disease resulting from impaired endothelial function, often caused by oxidant injury or inflammation. Endothelial progenitor cells (EPCs) play a critical role in repairing damaged endothelium and protecting against atherosclerosis. Quercitrin, a plant-derived flavonoid compound, displays antioxidant and anti-inflammatory activities. In this study, we showed that quercitrin treatment reduced the apoptosis of EPCs caused by oxidized low-density lipoprotein (ox-LDL) in a dose-dependent manner. Quercitrin improved tube formation, migration and adhesion of ox-LDL-treated EPCs. To determine the effect of quercitrin in vivo, EPCs treated with or without ox-LDL and quercitrin were locally injected into the ischemic hind limb muscle of nude mice. Those injected with EPCs treated with ox-LDL and quercitrin showed significantly increased local accumulation of EPCs, blood flow recovery and capillary density compared with the control and ox-LDL only groups. Furthermore, we showed that quercitrin enhanced autophagy and upregulated mitogen-activated protein kinase and ERK phosphorylation in a dose-dependent manner in vitro. Autophagy inhibitors, chloroquine and 3-methyladenine, abrogated quercitrin-enhanced autophagy caused by ox-LDL as evidenced by decreased numbers of branch points, migratory cells and adherent cells, and increased numbers of apoptotic cells. The ERK inhibitor PD98059 abrogated quercitrin-enhanced autophagy, as identified by decreased autophagosome formation and downregulated ERK phosphorylation. The inhibition of ERK did not affect the expression of Rac1, but enhanced phosphorylation of Akt. Quercitrin treatment also increased the expression of E-cadherin, and PD98059 abrogated the upregulation of E-cadherin induced by quercitrin. Our findings suggested that autophagy is a protective mechanism in EPCs exposed to oxidative damage. Quercitrin can promote autophagy through the activation of ERK and the ERK signaling pathway is therefore thought to play a pivotal role in mediating the protective effects on EPCs.
authors.affiliation: Department of Vascular and Endovascular Surgery, Changzheng Hospital, 415 Fengyang Road, Shanghai, 200003, China.
authors.forename: Kangkang
authors.initials: K
authors.lastname: Zhi
authors.affiliation: Department of Interventional Radiology, Tenth People's Hospital of Tongji University, 301 Yanchang Road, Shanghai, 200072, China.|Institute of Intervention Vessel, Tongji University, Shanghai, China.
authors.forename: Maoquan
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Vascular and Endovascular Surgery, Changzheng Hospital, 415 Fengyang Road, Shanghai, 200003, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Bai
authors.affiliation: Department of Vascular and Endovascular Surgery, Changzheng Hospital, 415 Fengyang Road, Shanghai, 200003, China.
authors.forename: Yongfa
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Vascular and Endovascular Surgery, Changzheng Hospital, 415 Fengyang Road, Shanghai, 200003, China.
authors.forename: Sili
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Interventional Radiology, Tenth People's Hospital of Tongji University, 301 Yanchang Road, Shanghai, 200072, China. zxpsibs@163.com.|Institute of Intervention Vessel, Tongji University, Shanghai, China. zxpsibs@163.com.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Vascular and Endovascular Surgery, Changzheng Hospital, 415 Fengyang Road, Shanghai, 200003, China. doctorqlf@163.com.
authors.forename: Lefeng
authors.initials: L
authors.lastname: Qu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology; D001343:Autophagy / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D066026:Endothelial Progenitor Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005121:Extremities / Q000098:blood supply; D005419:Flavonoids / Q000494:pharmacology; D007511:Ischemia / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D008077:Lipoproteins, LDL / Q000633:toxicity; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D018919:Neovascularization, Physiologic / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011794:Quercetin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D008077:Lipoproteins, LDL; D047428:Protein Kinase Inhibitors; C099252:oxidized low density lipoprotein; C012526:quercitrin; D011794:Quercetin; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one
keywords: Autophagy; ERK signaling pathway; Endothelial progenitor cells (EPCs); Oxidative damage; Quercitrin