title: Green synthesis of silver nanoparticles using Rheum palmatum root extract and their antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa.
abstract: In the present study, silver nanoparticles (AgNPs) were synthesized by using aqueous root extracts of Rheum palmatum and characterized by various spectroscopic methods. The nanoparticles were found to be in hexagonal and spherical shapes. The average particle size was found to be 121 ± 2 nm with zeta potential values of -21.6 mv by dynamic light scattering (DLS) method. Gas chromatography-mass spectrometry (GC-MS) analysis of R. palmatum revealed 35 compounds. The synthesized AgNPs showed significant activity against Staphylococcus aureus and Pseudomonas aeruginosa with IC90values of 15 μg/ml and IC50values of 7.5 μg/ml, respectively. The protein leakage level was high and morphological changes occurred in bacteria treated with AgNPs.
authors.affiliation: a Institute of Green Bioscience and Technology, Seoul National University , Pyeongchang , Republic of Korea.
authors.forename: Selvaraj
authors.initials: S
authors.lastname: Arokiyaraj
authors.affiliation: b Centre for Environmental Research and Development, Loyola College , Chennai , India.
authors.forename: Savariar
authors.initials: S
authors.lastname: Vincent
authors.affiliation: c Department of Medical Microbiology and Immunology , Institute of Biomedical Sciences, Mekelle University , Mekelle , Ethiopia.
authors.forename: Muthupandian
authors.initials: M
authors.lastname: Saravanan
authors.affiliation: a Institute of Green Bioscience and Technology, Seoul National University , Pyeongchang , Republic of Korea.
authors.forename: Yoonseok
authors.initials: Y
authors.lastname: Lee
authors.affiliation: d Department of Animal Nutrition and Physiology, National Institute of Animal Science , RDA, Jeonju , Republic of Korea.
authors.forename: Young Kyoon
authors.initials: YK
authors.lastname: Oh
authors.affiliation: a Institute of Green Bioscience and Technology, Seoul National University , Pyeongchang , Republic of Korea.|e Graduate School of International Agricultural Technology, Seoul National University , Pyeongchang , Republic of Korea.
authors.forename: Kyoung Hoon
authors.initials: KH
authors.lastname: Kim
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D053768:Metal Nanoparticles / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000254:growth & development*; D012250:Rheum / Q000737:chemistry*; D012834:Silver / Q000737:chemistry / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000254:growth & development*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D012834:Silver
keywords: Antibacterial; Rheum palmatum; SEM; XRD; green synthesis; silver nanoparticle