title: Ecdysis period and rate deviations of dengue mosquito vector, Aedes aegypti reared in different artificial water-holding containers.
abstract: OBJECTIVE
  Artificial water-holding containers (AWHCs) have been well-documented in many Aedes aegypti studies for dengue surveillance and developmental research. Hence, we investigated the role of different AHWCs on the development and ecdysis period of Ae. aegypti dengue vector, a container breeding mosquito.
  
  
  METHODS
  Nine types of AWHCs, namely glass, polystyrene foam, rubber, steel, porcelain, plastic, aluminum, clay and concrete, were chosen for the study. All AWHCs were subjected to the developmental assay for an observation period of 10 days. Regression and hazard analyses were employed to the developmental stages and the characteristics of the AWHCs.
  
  
  RESULTS
  The observations revealed that Ae. aegypti development is fastest in glass and polystyrene containers while slowest in concrete containers. Moreover, pupal ecdysis appears to be the most affected by the characteristics of the AWHCs based on regression and hazard analyses.
  
  
  CONCLUSIONS
  Characteristics of the container that can regulate water temperature seem to be the driving force with regards to the slow or fast development of Ae. aegypti, more notably in pupal ecdysis. The results of the study further strengthen our understanding on the dynamics of Ae. aegypti's developmental biology to different characteristics of artificial water containers. This, in turn, would aid in devising vector control strategies against dengue especially in endemic areas.
authors.forename: Beatriz Louise J
authors.initials: BL
authors.lastname: Almanzor
authors.forename: Howell T
authors.initials: HT
authors.lastname: Ho
authors.affiliation: Department of Civil and Environmental Engineering, Ehime University, Matsuyama, Japan and Biology Department, De La Salle University, Manila, Philippines, .
authors.forename: Thaddeus M
authors.initials: TM
authors.lastname: Carvajal
mesh_terms: D000330:Aedes / Q000254:growth & development / Q000502:physiology*; D000818:Animals; D007814:Larva / Q000254:growth & development; D018978:Molting; D000072138:Mosquito Vectors / Q000254:growth & development / Q000502:physiology*; D011679:Pupa / Q000254:growth & development; D013696:Temperature