title: Dynamics of the microbial community and Fe(III)-reducing and dechlorinating microorganisms in response to pentachlorophenol transformation in paddy soil.
abstract: Soil microorganisms play crucial roles in the fates of pollutants, and understanding the behaviour of these microorganisms is critical for the bioremediation of PCP-contaminated soil. However, shifts remain unclear in the community structure and Fe(III)-reducing and dechlorinating microorganisms during PCP transformation processes, especially during the stages from the lag to the dechlorination phase and from the dechlorination to the stationary phase. Here, a set of lab-scale experiments was performed to investigate the microbial community dynamics accompanying PCP transformation in paddy soil. 19μM of PCP was biotransformed completely in 10days for all treatments. T-RFLP analysis of the microbial community confirmed that Veillonellaceae and Clostridium sensu stricto were the dominant groups during PCP transformation, and the structures of the microbial communities changed due to the degree of biotransformation and the addition of lactate and AQDS. However, similar temporal dynamics of the microbial communities were obtained among all treatments. Furthermore, as revealed by quantitative PCR, the dynamics of Fe(III)-reducing and dechlorinating microorganisms, including Geobacter sp., Shewanella sp., and Dehalobacter sp., were consistent with the transformation kinetics of PCP, suggesting the critical roles played by these microorganisms in PCP transformation. These findings are valuable for making predictions of and proposing methods for the microbial detoxification of residual organochlorine pesticides in paddy soil.
authors.affiliation: Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, China.
authors.forename: Manjia
authors.initials: M
authors.lastname: Chen
authors.affiliation: Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, China; State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550009, China. Electronic address: liuchengshuai@vip.gyig.ac.cn.
authors.forename: Chengshuai
authors.initials: C
authors.lastname: Liu
authors.affiliation: Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, China.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Chen
authors.affiliation: Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, China; State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550009, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Tong
authors.affiliation: Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, China. Electronic address: cefbli@soil.gd.cn.
authors.forename: Fangbai
authors.initials: F
authors.lastname: Li
authors.affiliation: Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, China.
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Qiao
authors.affiliation: Guangdong Polytechnic of Environmental Protection Engineering, Foshan, Guangdong 528216, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Lan
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D005290:Ferric Compounds / Q000737:chemistry*; D006843:Hydrocarbons, Chlorinated / Q000737:chemistry; D012275:Oryza; D010416:Pentachlorophenol / Q000737:chemistry*; D010575:Pesticides / Q000737:chemistry; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000737:chemistry*
chemical_list: D005290:Ferric Compounds; D006843:Hydrocarbons, Chlorinated; D010575:Pesticides; D012989:Soil Pollutants; D010416:Pentachlorophenol
keywords: Dynamics; Microbial community structure; Pentachlorophenol; T-RFLP