title: Protective effect of Dunaliella sp., lipid extract rich in polyunsaturated fatty acids, on hepatic and renal toxicity induced by nickel in rats.
abstract: OBJECTIVE
  The present study was undertaken to investigate the protective effect of lipid extract of Dunaliella sp. (LE) rich in polyunsaturated fatty acids (PUFA), against oxidative stress induced by nickel in experimental rats.
  
  
  METHODS
  Our investigation evaluated the antioxidant activity of LE using both DPPH and NBT assays. Twenty female albino Wistar rats, randomly allocated into four experimental groups, namely (C): control, (Nit): nickel-treated rats with 5 mg/kg/d of NiCl2 during 30 days, (LEa): lipid extract-administered rats with 5  mg/kg BW/d during 30 days and (Nit + LEa): rats treated with Ni and LE-administered during 30 days.
  
  
  RESULTS
  The in vitro antioxidant activity demonstrated that LE presents an important antioxidant potential. In vivo, the (Nit + LEa) cotreatment decreased the level of malondialdehyde and restored the antioxidant enzyme activities (superoxide dismutase, catalase and glutathione peroxidase) in livers and kidneys in comparison with those treated with Ni only. LE administration to rats treated with Ni also ameliorated biochemical and histological parameters as compared to only Ni-treated group. LE of Dunaliella sp., rich in polyunsaturated fatty acids showed a significant hepato- and reno-protective effect against metal-induced toxicity.
  
  
  CONCLUSIONS
  LE of Dunaliella sp., rich in PUFA has been proven to be effective in protection against Ni-induced toxicity.
authors.affiliation: a Laboratory of Environmental Bioprocesses , Centre of Biotechnology of Sfax, University of Sfax , Sfax Tunisia .
authors.forename: Ines
authors.initials: I
authors.lastname: Dahmen-Ben Moussa
authors.affiliation: b Department of life Sciences, Laboratory of Animal Ecophysiology, Faculty of Sciences of Sfax , University of Sfax , Sfax , Tunisia .
authors.forename: Khaled
authors.initials: K
authors.lastname: Bellassoued
authors.affiliation: b Department of life Sciences, Laboratory of Animal Ecophysiology, Faculty of Sciences of Sfax , University of Sfax , Sfax , Tunisia .|c Department of life Sciences, Aquatic Ecosystem Biodiversity Research Unit, Faculty of Sciences of Sfax , University of Sfax , Sfax , Tunisia , and.
authors.forename: Khaled
authors.initials: K
authors.lastname: Athmouni
authors.affiliation: d Laboratory of Biochemistry , CHU Habib-Bourguiba , Sfax , Tunisia.
authors.forename: Manel
authors.initials: M
authors.lastname: Naifar
authors.affiliation: a Laboratory of Environmental Bioprocesses , Centre of Biotechnology of Sfax, University of Sfax , Sfax Tunisia .
authors.forename: Haifa
authors.initials: H
authors.lastname: Chtourou
authors.affiliation: c Department of life Sciences, Aquatic Ecosystem Biodiversity Research Unit, Faculty of Sciences of Sfax , University of Sfax , Sfax , Tunisia , and.
authors.forename: Habib
authors.initials: H
authors.lastname: Ayadi
authors.affiliation: d Laboratory of Biochemistry , CHU Habib-Bourguiba , Sfax , Tunisia.
authors.forename: Fatma
authors.initials: F
authors.lastname: Makni-Ayadi
authors.affiliation: a Laboratory of Environmental Bioprocesses , Centre of Biotechnology of Sfax, University of Sfax , Sfax Tunisia .
authors.forename: Sami
authors.initials: S
authors.lastname: Sayadi
authors.affiliation: b Department of life Sciences, Laboratory of Animal Ecophysiology, Faculty of Sciences of Sfax , University of Sfax , Sfax , Tunisia .
authors.forename: Abdelfatteh
authors.initials: A
authors.lastname: El Feki
authors.affiliation: a Laboratory of Environmental Bioprocesses , Centre of Biotechnology of Sfax, University of Sfax , Sfax Tunisia .
authors.forename: Abdelhafidh
authors.initials: A
authors.lastname: Dhouib
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D001835:Body Weight / Q000187:drug effects; D000460:Chlorophyta / Q000737:chemistry*; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005260:Female; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D007677:Kidney Function Tests; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D008111:Liver Function Tests; D009532:Nickel / Q000633:toxicity*; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D005231:Fatty Acids, Unsaturated; D010851:Picrates; D010936:Plant Extracts; D009532:Nickel; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Kidney; PUFA; liver; nickel; oxidative stress