title: Coexistence of two forms of disease-associated prion protein in extracerebral tissues of cattle infected with H-type bovine spongiform encephalopathy.
abstract: H-type bovine spongiform encephalopathy (H-BSE) is an atypical form of BSE in aged cattle. H-BSE is characterized by the presence of two proteinase K-resistant forms of disease-associated prion protein (PrP(Sc)), identified as PrP(Sc) #1 and PrP(Sc) #2, in the brain. To investigate the coexistence of different PrP(Sc) forms in the extracerebral tissues of cattle experimentally infected with H-BSE, immunohistochemical and molecular analyses were performed by using N-terminal-, core-region- and C-terminal-specific anti-prion protein antibodies. Our results demonstrated that two distinct forms of PrP(Sc) coexisted in the various extracerebral tissues.
authors.affiliation: National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Okada
authors.forename: Kohtaro
authors.initials: K
authors.lastname: Miyazawa
authors.forename: Kentaro
authors.initials: K
authors.lastname: Masujin
authors.forename: Takashi
authors.initials: T
authors.lastname: Yokoyama
mesh_terms: D000818:Animals; D002417:Cattle; D016643:Encephalopathy, Bovine Spongiform / Q000378:metabolism*; D007150:Immunohistochemistry; D018620:PrPSc Proteins / Q000032:analysis* / Q000737:chemistry; D014018:Tissue Distribution
chemical_list: D018620:PrPSc Proteins