title: Theranostic MUC-1 aptamer targeted gold coated superparamagnetic iron oxide nanoparticles for magnetic resonance imaging and photothermal therapy of colon cancer.
abstract: Favorable physiochemical properties and the capability to accommodate targeting moieties make superparamegnetic iron oxide nanoparticles (SPIONs) popular theranostic agents. In this study, we engineered SPIONs for magnetic resonance imaging (MRI) and photothermal therapy of colon cancer cells. SPIONs were synthesized by microemulsion method and were then coated with gold to reduce their cytotoxicity and to confer photothermal capabilities. Subsequently, the NPs were conjugated with thiol modified MUC-1 aptamers. The resulting NPs were spherical, monodisperse and about 19nm in size, as shown by differential light scattering (DLS) and transmission electron microscopy (TEM). UV and X-ray photoelectron spectroscopy (XPS) confirmed the successful gold coating. MTT results showed that Au@SPIONs have insignificant cytotoxicity at the concentration range of 10-100μg/ml (P>0.05) and that NPs covered with protein corona exerted lower cytotoxicity than bare NPs. Furthermore, confocal microscopy confirmed the higher uptake of aptamer-Au@SPIONs in comparison with non-targeted SPIONs. MR imaging revealed that SPIONs produced significant contrast enhancement in vitro and they could be exploited as contrast agents. Finally, cells treated with aptamer-Au@SPIONs exhibited a higher death rate compared to control cells upon exposure to near infrared light (NIR). In conclusion, MUC1-aptamer targeted Au@SPIONs could serve as promising theranostic agents for simultaneous MR imaging and photothermal therapy of cancer cells.
authors.affiliation: Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 1417614411, Iran; Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 1417614411, Iran.
authors.forename: Morteza
authors.initials: M
authors.lastname: Azhdarzadeh
authors.affiliation: Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 1417614411, Iran; Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 1417614411, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Atyabi
authors.affiliation: Department of Medical Biochemistry & Biophysics, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Amir Ata
authors.initials: AA
authors.lastname: Saei
authors.affiliation: Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 1417614411, Iran; Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 1417614411, Iran.
authors.forename: Behrang Shiri
authors.initials: BS
authors.lastname: Varnamkhasti
authors.affiliation: Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Yadollah
authors.initials: Y
authors.lastname: Omidi
authors.affiliation: Medical Laser Research Center, Academic Center for Education, Culture, and Research (ACECR), Tehran, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Fateh
authors.affiliation: Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, Health Science Faculty, Blegdamsvej 3c, 2200 Copenhagen N, Denmark.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Ghavami
authors.affiliation: Nuclear Science and Technology Research Center (NSTRI), Tehran, Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Shanehsazzadeh
authors.affiliation: Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 1417614411, Iran; Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 1417614411, Iran. Electronic address: dinarvand@tums.ac.ir.
authors.forename: Rassoul
authors.initials: R
authors.lastname: Dinarvand
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D016466:CHO Cells; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D006224:Cricetinae; D003412:Cricetulus; D006046:Gold / Q000737:chemistry*; D019073:HT29 Cells; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D018396:Mucin-1 / Q000737:chemistry* / Q000378:metabolism; D010778:Photochemotherapy / Q000379:methods*; D056951:Photoelectron Spectroscopy; D000068936:Theranostic Nanomedicine / Q000379:methods
chemical_list: D052157:Aptamers, Nucleotide; D020099:Coated Materials, Biocompatible; D058185:Magnetite Nanoparticles; D018396:Mucin-1; D006046:Gold
keywords: MRI; MUC1 aptamer; Nanoparticles; Protein corona; SPION; Theranostics