title: Phylogenetic characterization of a novel herpesvirus found in the liver and lungs of a Chilean flamingo (Phoenicopterus chilensis).
abstract: A novel herpesvirus was detected in a 17-day-old Chilean flamingo (Phoenicopterus chilensis) with pneumonia, hepatopathy, and severe anemia that was housed in California. Postmortem examination identified a pale, enlarged liver, mildly increased fluid in the lungs, and red foci in the spleen. Histologic examination revealed marked hepatic necrosis with syncytia, splenic necrosis, and interstitial pneumonia with eosinophilic intranuclear inclusions within hepatocytes and in unidentified cells of the lung. Transmission electron microscopy identified virions consistent with a herpesvirus in the nucleus and cytoplasm of degenerative hepatocytes. Nested consensus PCR, sequencing, and phylogenetic analysis identified a novel herpesvirus within the genus Iltovirus in the subfamily Alphaherpesvirinae.
authors.affiliation: College of Veterinary Medicine, University of Florida, Gainesville, FL (Coverdill, Childress, Wellehan)Santa Barbara Zoo, Santa Barbara, CA (Barnes)Northwest ZooPath, Monroe WA (Garner)Joint Pathology Center, Silver Spring, MD (Hinton).
authors.forename: Christopher C
authors.initials: CC
authors.lastname: Coverdill
authors.affiliation: College of Veterinary Medicine, University of Florida, Gainesville, FL (Coverdill, Childress, Wellehan)Santa Barbara Zoo, Santa Barbara, CA (Barnes)Northwest ZooPath, Monroe WA (Garner)Joint Pathology Center, Silver Spring, MD (Hinton).
authors.forename: Julie A
authors.initials: JA
authors.lastname: Barnes
authors.affiliation: College of Veterinary Medicine, University of Florida, Gainesville, FL (Coverdill, Childress, Wellehan)Santa Barbara Zoo, Santa Barbara, CA (Barnes)Northwest ZooPath, Monroe WA (Garner)Joint Pathology Center, Silver Spring, MD (Hinton).
authors.forename: Michael M
authors.initials: MM
authors.lastname: Garner
authors.affiliation: College of Veterinary Medicine, University of Florida, Gainesville, FL (Coverdill, Childress, Wellehan)Santa Barbara Zoo, Santa Barbara, CA (Barnes)Northwest ZooPath, Monroe WA (Garner)Joint Pathology Center, Silver Spring, MD (Hinton).
authors.forename: Kevin L
authors.initials: KL
authors.lastname: Hinton
authors.affiliation: College of Veterinary Medicine, University of Florida, Gainesville, FL (Coverdill, Childress, Wellehan)Santa Barbara Zoo, Santa Barbara, CA (Barnes)Northwest ZooPath, Monroe WA (Garner)Joint Pathology Center, Silver Spring, MD (Hinton).
authors.forename: April L
authors.initials: AL
authors.lastname: Childress
authors.affiliation: College of Veterinary Medicine, University of Florida, Gainesville, FL (Coverdill, Childress, Wellehan)Santa Barbara Zoo, Santa Barbara, CA (Barnes)Northwest ZooPath, Monroe WA (Garner)Joint Pathology Center, Silver Spring, MD (Hinton) wellehanj@ufl.edu.
authors.forename: James F X
authors.initials: JF
authors.lastname: Wellehan
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000175:diagnosis* / Q000473:pathology / Q000821:virology; D001717:Birds; D002140:California; D006564:Herpesviridae / Q000235:genetics / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D008099:Liver / Q000821:virology; D008168:Lung / Q000821:virology; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary
keywords: Chilean flamingos; Iltovirus; Phoenicopterus chilensis; herpesvirus