title: First molecular survey and identification of Anaplasma spp. in white yaks (Bos grunniens) in China.
abstract: Anaplasmosis is caused by a group of obligate intracellular bacteria in the genus Anaplasma, which are transmitted by ticks and infect humans, domestic animals and wildlife. This study was conducted to determine the prevalence and molecular characterization of Anaplasma spp. in semi-wild white yaks sampled in Tianzhu Tibetan Autonomous County, northwest China. Out of 332 samples tested, 35 (10·9%) were positive for Anaplasma spp. The positive rates were 6·2% (20/322) and 5·3% (17/322) for Anaplasma bovis and Anaplasma phagocytophilum in white yaks, respectively. None of the sample was positive for Anaplasma marginale. Two (0·6%) samples were simultaneously positive to A. bovis and A. phagocytophilum. Sequence analysis of the 16S rRNA gene revealed two genotypes (ApG1 and ApG2) of A. phagocytophilum and two sequence types (ST1 and ST2) of A. bovis in white yaks. This study is the first to document the presence of Anaplasma in white yaks. Our findings extend the host range for Anaplasma species and provide more valuable information for the control and management of anaplasmosis in white yaks.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Science,Xujiaping 1,Lanzhou,Gansu 730046,People's Republic of China.
authors.forename: Jifei
authors.initials: J
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Science,Xujiaping 1,Lanzhou,Gansu 730046,People's Republic of China.
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Science,Xujiaping 1,Lanzhou,Gansu 730046,People's Republic of China.
authors.forename: Qingli
authors.initials: Q
authors.lastname: Niu
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Science,Xujiaping 1,Lanzhou,Gansu 730046,People's Republic of China.
authors.forename: Junlong
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Science,Xujiaping 1,Lanzhou,Gansu 730046,People's Republic of China.
authors.forename: Guiquan
authors.initials: G
authors.lastname: Guan
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Science,Xujiaping 1,Lanzhou,Gansu 730046,People's Republic of China.
authors.forename: Jingying
authors.initials: J
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Science,Xujiaping 1,Lanzhou,Gansu 730046,People's Republic of China.
authors.forename: Jianxun
authors.initials: J
authors.lastname: Luo
authors.affiliation: Animal Diseases Control and Prevention Centre of Tianzhu county,Tianzhu, Gansu 733299,People's Republic of China.
authors.forename: Shuqing
authors.initials: S
authors.lastname: Wang
authors.affiliation: Animal Diseases Control and Prevention Centre of Tianzhu county,Tianzhu, Gansu 733299,People's Republic of China.
authors.forename: Shufang
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Science,Xujiaping 1,Lanzhou,Gansu 730046,People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yin
mesh_terms: D000709:Anaplasma / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D000712:Anaplasmosis / Q000453:epidemiology* / Q000382:microbiology*; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000382:microbiology*; D002681:China / Q000453:epidemiology; D005838:Genotype; D015995:Prevalence; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA gene; Anaplasma bovis; Anaplasma phagocytophilum; tick-borne disease; white yak