title: The prognostic role of Eg5 expression in laryngeal squamous cell carcinoma.
abstract: Eg5 is identified as a microtubule-dependent motor protein and has been suggested to play important role in cancer development. The aim of this study was to detect the expression of Eg5 and its clinicopathological characteristics in patients with laryngeal squamous cell carcinoma. One-step quantitative reverse transcription-polymerase chain reaction (qPCR) test with 20 fresh-frozen LSCC samples and immunohistochemistry (IHC) analysis in 137 LSCC cases were performed to investigate the relationship between Eg5 expression and the clinicopathological characteristics of LSCC. The Eg5 mRNA and protein levels were significantly higher in LSCC tissues compared with corresponding non-cancerous tissues (p < 0.05). Eg5 protein expression was significantly correlated to lymph node metastasis (p = 0.021) and TNM stage (p = 0.030). Kaplan-Meier survival and multivariate analysis with Cox regression model indicated that high Eg5 expression (p = 0.031) and TNM stage (p = 0.011) were independent factors to predict unfavourable prognosis for patients with LSCC. The data suggested that Eg5 may be identified as a novel prognostic biomarker and targeting Eg5 seems to be a novel strategy for LSCC treatment.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Meiping
authors.initials: M
authors.lastname: Lu
authors.affiliation: Department of Pathology, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Pathology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xiong
authors.affiliation: Department of Pathology, Jiangsu Cancer Hospital, Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Affiliated Hospital of Nantong University, Nantong, China. Electronic address: ywyount@126.com.
authors.forename: Yiwen
authors.initials: Y
authors.lastname: You
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D016547:Kinesins / Q000235:genetics / Q000378:metabolism*; D007822:Laryngeal Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology; D008207:Lymphatic Metastasis; D008875:Middle Aged; D011379:Prognosis; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046888:Tissue Array Analysis
chemical_list: D014408:Biomarkers, Tumor; C470243:KIF11 protein, human; D016547:Kinesins
keywords: Eg5; IHC; LSCC; qPCR