title: Expression ratio of histone demethylase KDM3A to protamine-1 mRNA is predictive of successful testicular sperm extraction in men with obstructive and non-obstructive azoospermia.
abstract: To evaluate the predictive value of histone demethylase KDM3A to protamine 1 (PRM1) mRNA expression ratio as a reliable marker of sperm retrieval in men with obstructive and non-obstructive azoospermia (NOA). Fifty eight azoospermic men, including 44 with NOA and 14 with obstructive azoospermia (OA). Testis tissue samples were collected from azoospermic men who have been referred for testicular sperm extraction (TESE) and micro-TESE. Relative expression ratio of KDM3A to PRM1 was analyzed after selection of approved reference genes. Histological classification of testis biopsies was performed. Sperm retrieval following TESE and micro-TESE was evaluated. A sperm retrieval prediction sensitivity of 95% was established when the Cq of PRM1 became smaller than the Cq of both KDM3A and GAPDH genes. However, azoospermic men with down-regulated KDM3A and decreased expression of PRM1 mRNA showed very low success for sperm retrieval (<25%), even after micro-TESE surgery. The KDM3A to PRM1 mRNA expression ratio can be used as a reliable marker of successful testicular sperm extraction in men with obstructive and non-obstructive azoospermia with 95% sensitivity.
authors.affiliation: Department of Biology, University of Isfahan, Isfahan, Iran.
authors.forename: S M
authors.identifier: 0000-0002-2891-2293
authors.initials: SM
authors.lastname: Javadirad
authors.affiliation: Department of Biology, University of Isfahan, Isfahan, Iran.
authors.forename: Z
authors.initials: Z
authors.lastname: Hojati
authors.affiliation: Department of Biology, University of Isfahan, Isfahan, Iran.|Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
authors.forename: K
authors.initials: K
authors.lastname: Ghaedi
authors.affiliation: Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.|Isfahan Fertility and Infertility Center, IFIC, Isfahan, Iran.
authors.forename: M H
authors.initials: MH
authors.lastname: Nasr-Esfahani
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D056484:Jumonji Domain-Containing Histone Demethylases / Q000235:genetics / Q000378:metabolism*; D008297:Male; D011379:Prognosis; D011479:Protamines / Q000235:genetics / Q000378:metabolism*; D053625:Sperm Retrieval; D013094:Spermatozoa / Q000378:metabolism*; D013737:Testis / Q000473:pathology*; D016896:Treatment Outcome
chemical_list: C082073:PRM1 protein, human; D011479:Protamines; D056484:Jumonji Domain-Containing Histone Demethylases; C530247:KDM3A protein, human
keywords: KDM3A; PRM1; azoospermia; sperm retrieval