title: Expression of death receptor 3 on peripheral blood mononuclear cells differes in adult IBD patients and children with newly diagnosed IBD.
abstract: Interaction between TL1A and death receptor 3 (DR3) is associated with the pathogenesis of inflammatory bowel disease (IBD), although their role in the development of this disease remains not fully explained. Some studies showed elevated expression of TL1A and DR3 in inflamed intestinal tissue but currently there are no reports concerning expression of DR3 on peripheral blood mononuclear cells (PBMCs) of IBD patients which was the subject of our study.
  
  
  
  We performed flow cytometry analysis of DR3 expression on CD4(+), CD8(+), CD11c(+), CD14(+) or CD20(+) PBMCs of adults and children with IBD and healthy volunteers with respect to C-reactive protein (CRP) levels in blood. Blood samples were collected from pediatric patients before the beginning of therapy, whereas adults patients were undergoing anti-inflammatory IBD treatment and had much lower CRP levels.
  
  
  
  With regard to appropriate healthy volunteers, children with IBD had elevated percentage of DR3-expressing CD4(+), CD8(+), CD11c(+) and CD20(+) PBMCs which, with the exception of DR3(+) CD11c(+) cells in children with ulcerative colitis, was correlated with CRP level in blood. Adult patients had increased frequency of DR3(+) CD8(+) and CD20(+) PBMCs and their CRP levels correlated only with DR3(+) CD8(+) cells.
  
  
  
  In comparison to healthy volunteers, untreated children with IBD have higher percentage of DR3(+) PBMCs than adults with IBD undergoing anti-inflammatory treatment. In most of the investigated PBMCs populations, the frequency of DR3(+) cells is correlated with the level of CRP. We suggest anti-inflammatory treatment may lead to reduction in the frequency of DR3(+) PBMCs. © 2016 International Clinical Cytometry Society.
authors.affiliation: Department of Histology, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Tomasz J
authors.initials: TJ
authors.lastname: Slebioda
authors.affiliation: Department of Clinical Immunology, Medical University of Lublin, Lublin, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Bojarska-Junak
authors.affiliation: Department of Histology, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Cyman
authors.affiliation: Clinic of Paediatrics, Gastroenterology, Hepatology and Paediatric Nutrition, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Landowski
authors.affiliation: Clinic of Paediatrics, Gastroenterology, Hepatology and Paediatric Nutrition, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Kaminska
authors.affiliation: Department of Gastroenterology with Endoscopic Unit, Medical University of Lublin, Lublin, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Celinski
authors.affiliation: Department of Histology, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Kmiec
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D002097:C-Reactive Protein / Q000378:metabolism; D002648:Child; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D015212:Inflammatory Bowel Diseases / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D053219:Receptors, Tumor Necrosis Factor, Member 25 / Q000378:metabolism*; D053380:Tumor Necrosis Factor Ligand Superfamily Member 15 / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D053219:Receptors, Tumor Necrosis Factor, Member 25; C506033:TNFRSF25 protein, human; C117026:TNFSF15 protein, human; D053380:Tumor Necrosis Factor Ligand Superfamily Member 15; D002097:C-Reactive Protein
keywords: TL1A; death receptor 3; inflammatory bowel disease; peripheral blood mononuclear cells