title: MMP9 Promoter Polymorphism (-1562 C/T) Does not Affect the Serum Levels of Soluble MICB and MICA in Breast Cancer.
abstract: BACKGROUND
  The role of Matrix Metalloproteinase 9 (MMP9) in tumor invasion and progression is prominent. A single nucleotide polymorphism (SNP) in the promoter region of MMP9 (-1562 C/T) increases the transcription and expression of this gene. On the other hand, MHC class I chain-related protein A and B (MICA/B) in soluble forms may impair tumor immunogenicity by reducing Natural Killer Group 2D (NKG2D) densities on NK cells and MMP9 enzyme activity has a prominent role in shedding of MICA/B.
  
  
  OBJECTIVE
  To investigate the association between MMP9 (-1562 C/T) polymorphism and serum MICA/B level in breast cancer patients.
  
  
  METHODS
  In this case-control study, 105 patients with breast cancer and 100 healthy age-matched women were selected from Yazd hospitals, Iran. The polymorphism of MMP9 (-1562 C/T) was determined by PCR-RFLP. Concentration of MICB and MICA in the sera of breast cancer patients and healthy women were measured using ELISA method.
  
  
  RESULTS
  The frequency of CC, CT and TT genotypes and T allele of the MMP9 (-1562 C/T) did not show significant differences between breast cancer patients and healthy donors (p>0.05). On the other hand, the mean serum levels of MICB and MICA were significantly elevated in patients compared with healthy individuals (p<0.05). In patients with MMP9CC genotype, the mean serum MICB concentration was significantly higher than those patients with CT polymorphism (p<0.05). Although the mean of blood MICA concentration in patients with the CT genotype was higher than those patients with CC genotype, the difference was not statistically significant.
  
  
  CONCLUSIONS
  The T allele of the MMP9 (-1562 C/T) does not show a correlation with serum levels of MICA and MICB in breast cancer patients.
authors.affiliation: Department of Immunology, International Campus, Shahid Sadoughi University Medical Sciences, Yazd, Iran, e-mail: mohamadhasanbargostavan@yahoo.com.
authors.forename: Mohammad Hasan
authors.initials: MH
authors.lastname: Bargostavan
authors.forename: Gilda
authors.initials: G
authors.lastname: Eslami
authors.forename: Nasrin
authors.initials: N
authors.lastname: Esfandiari
authors.forename: Ali
authors.initials: A
authors.lastname: Shams Shahemabadi
mesh_terms: D000328:Adult; D000483:Alleles; D001943:Breast Neoplasms / Q000097:blood* / Q000235:genetics* / Q000276:immunology; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D015395:Histocompatibility Antigens Class I / Q000097:blood* / Q000276:immunology; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic
chemical_list: D015395:Histocompatibility Antigens Class I; C099187:MHC class I-related chain A; C098080:MICB antigen; D020780:Matrix Metalloproteinase 9