title: siRNA knockdown validation 101: Incorporating negative controls in antibody research.
abstract: More antibody validation protocols would identify non-specific reagents - a major source of irreproducible research - with the inclusion of negative controls. This article presents an overview of one such method: siRNA knockdown. The authors outline a general protocol, the knockdown mechanism, and tips for evaluating knockdown experiments.
authors.affiliation: Proteintech Group, Rosemont, IL, USA.
authors.forename: Will
authors.initials: W
authors.lastname: Olds
authors.affiliation: Proteintech Group, Rosemont, IL, USA.
authors.forename: Jason
authors.initials: J
authors.lastname: Li
keywords: Western Blot; knockdown; negative control; shRNA; siRNA; specificity; transfection; vector