title: Enhancement of Lytic Activity by Leptin Is Independent From Lipid Rafts in Murine Primary Splenocytes.
abstract: Leptin, a pleiotropic adipokine, is known as a regulator of food intake, but it is also involved in inflammation, immunity, cell proliferation, and survival. Leptin receptor is integrated inside cholesterol-rich microdomains called lipid rafts, which, if disrupted or destroyed, could lead to a perturbation of lytic mechanism. Previous studies also reported that leptin could induce membrane remodeling. In this context, we studied the effect of membrane remodeling in lytic activity modulation induced by leptin. Thus, primary mouse splenocytes were incubated with methyl-β-cyclodextrin (β-MCD), a lipid rafts disrupting agent, cholesterol, a major component of cell membranes, or ursodeoxycholic acid (UDCA), a membrane stabilizer agent for 1 h. These treatments were followed by splenocyte incubation with leptin (absence, 10 and 100 ng/ml). Unlike β-MCD or cholesterol, UDCA was able to block leptin lytic induction. This result suggests that leptin increased the lytic activity of primary spleen cells against syngenic EO771 mammary cancer cells independently from lipid rafts but may involve membrane fluidity. Furthermore, natural killer cells were shown to be involved in the splenocyte lytic activity. To our knowledge it is the first publication in primary culture that provides the link between leptin lytic modulation and membrane remodeling. J. Cell. Physiol. 232: 101-109, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Clermont Université, Université d'Auvergne, UFR Pharmacie, UMR 1019, Unité de Nutrition Humaine, Equipe ECREIN, CLARA, Clermont-Ferrand, France. aurore.collin@udamail.fr.|INRA, UMR 1019, Unité de Nutrition Humaine, CRNH Auvergne, Clermont-Ferrand, France. aurore.collin@udamail.fr.
authors.forename: Aurore
authors.initials: A
authors.lastname: Collin
authors.affiliation: Clermont Université, Université d'Auvergne, UFR Pharmacie, UMR 1019, Unité de Nutrition Humaine, Equipe ECREIN, CLARA, Clermont-Ferrand, France.|INRA, UMR 1019, Unité de Nutrition Humaine, CRNH Auvergne, Clermont-Ferrand, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Noacco
authors.affiliation: Clermont Université, Université d'Auvergne, UFR Pharmacie, UMR 1019, Unité de Nutrition Humaine, Equipe ECREIN, CLARA, Clermont-Ferrand, France.|INRA, UMR 1019, Unité de Nutrition Humaine, CRNH Auvergne, Clermont-Ferrand, France.
authors.forename: Jérémie
authors.initials: J
authors.lastname: Talvas
authors.affiliation: Clermont Université, Université d'Auvergne, UFR Pharmacie, UMR 1019, Unité de Nutrition Humaine, Equipe ECREIN, CLARA, Clermont-Ferrand, France.|INRA, UMR 1019, Unité de Nutrition Humaine, CRNH Auvergne, Clermont-Ferrand, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Caldefie-Chézet
authors.affiliation: Clermont Université, Université d'Auvergne, UFR Pharmacie, UMR 1019, Unité de Nutrition Humaine, Equipe ECREIN, CLARA, Clermont-Ferrand, France.|INRA, UMR 1019, Unité de Nutrition Humaine, CRNH Auvergne, Clermont-Ferrand, France.|Centre Jean-Perrin, CHU Gabriel-Montpied, Unité de Nutrition, Clermont-Ferrand, France.
authors.forename: Marie-Paule
authors.initials: MP
authors.lastname: Vasson
authors.affiliation: Clermont Université, Université d'Auvergne, UFR Pharmacie, UMR 1019, Unité de Nutrition Humaine, Equipe ECREIN, CLARA, Clermont-Ferrand, France.|INRA, UMR 1019, Unité de Nutrition Humaine, CRNH Auvergne, Clermont-Ferrand, France.
authors.forename: Marie-Chantal
authors.initials: MC
authors.lastname: Farges
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism; D005260:Female; D007694:Killer Cells, Natural / Q000378:metabolism; D020738:Leptin / Q000378:metabolism*; D021962:Membrane Microdomains / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000378:metabolism*; D047392:beta-Cyclodextrins / Q000378:metabolism
chemical_list: D020738:Leptin; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; D002784:Cholesterol