title: Proteomic analysis to elucidate degeneration of Clostridium beijerinckii NCIMB 8052 and role of Ca(2+) in strain recovery from degeneration.
abstract: Degeneration of solventogenic Clostridium strains is one of the major barriers in bio-butanol production. A degenerated Clostridium beijerinckii NCIMB 8052 strain (DG-8052) was obtained without any genetic manipulation. Supplementation of CaCO3 to fermentation medium could partially recover metabolism of DG-8052 by more than 50 % increase of cell growth and solvent production. This study investigated the protein expression profile of DG-8052 and its response to CaCO3 treatment. Compared with WT-8052, the lower expressed proteins were responsible for disruption of RNA secondary structures and DNA repair, sporulation, signal transduction, transcription regulation, and membrane transport in DG-8052. Interestingly, accompanied with the decreased glucose utilization and lower solvent production, there was a decreased level of sigma-54 modulation protein which may indicate that the level of sigma-54 activity may be associated with the observed strain degeneration. For the addition of CaCO3, proteomic and biochemical study results revealed that besides buffer capacity, Ca(2+) could stabilize heat shock proteins, increase DNA synthesis and replication, and enhance expression of solventogenic enzymes in DG-8052, which has a similar contribution in WT-8052.
authors.affiliation: School of Public Health, Health Science Center, Xi'an Jiaotong University, NO. 76 Yanta West Road, P.O. 44, Xi'an, 710061, Shaanxi, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Lv
authors.affiliation: School of Public Health, Health Science Center, Xi'an Jiaotong University, NO. 76 Yanta West Road, P.O. 44, Xi'an, 710061, Shaanxi, China.
authors.forename: Shengyin
authors.initials: S
authors.lastname: Jiao
authors.affiliation: School of Public Health, Health Science Center, Xi'an Jiaotong University, NO. 76 Yanta West Road, P.O. 44, Xi'an, 710061, Shaanxi, China.
authors.forename: Renjia
authors.initials: R
authors.lastname: Du
authors.affiliation: School of Public Health, Health Science Center, Xi'an Jiaotong University, NO. 76 Yanta West Road, P.O. 44, Xi'an, 710061, Shaanxi, China.|Nutrition and Food Safety Engineering Research Center of Shaanxi Province, Xi'an, China.
authors.forename: Ruijuan
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Public Health, Health Science Center, Xi'an Jiaotong University, NO. 76 Yanta West Road, P.O. 44, Xi'an, 710061, Shaanxi, China. hanbei@mail.xjtu.edu.cn.|Nutrition and Food Safety Engineering Research Center of Shaanxi Province, Xi'an, China. hanbei@mail.xjtu.edu.cn.
authors.forename: Bei
authors.initials: B
authors.lastname: Han
mesh_terms: D000440:Butanols / Q000378:metabolism; D002118:Calcium / Q000737:chemistry*; D002119:Calcium Carbonate / Q000378:metabolism; D046970:Clostridium beijerinckii / Q000235:genetics / Q000254:growth & development*; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D007218:Industrial Microbiology; D040901:Proteomics; D059467:Transcriptome
chemical_list: D000440:Butanols; D003470:Culture Media; D002119:Calcium Carbonate; D002118:Calcium
keywords: Ca2+ contribution; Clostridium beijerinckii; Degeneration; Proteomic analysis; Solvent production