title: Dissecting microRNA-mediated regulation of stemness, reprogramming, and pluripotency.
abstract: Increasing evidence indicates that microRNAs (miRNAs), endogenous short non-coding RNAs 19-24 nucleotides in length, play key regulatory roles in various biological events at the post-transcriptional level. Embryonic stem cells (ESCs) represent a valuable tool for disease modeling, drug discovery, developmental studies, and potential cell-based therapies in regenerative medicine due to their unlimited self-renewal and pluripotency. Therefore, remarkable progress has been made in recent decades toward understanding the expression and functions of specific miRNAs in the establishment and maintenance of pluripotency. Here, we summarize the recent knowledge regarding the regulatory roles of miRNAs in self-renewal of pluripotent ESCs and during cellular reprogramming, as well as the potential role of miRNAs in two distinct pluripotent states (naïve and primed).
authors.affiliation: iDream Research Center, MizMedi Women's Hospital, Seoul, 07639 South Korea.
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Department of Biomedical Science, Graduate School of Biomedical Science and Engineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul, 04763 South Korea.|College of Medicine, Hanyang University, Seoul, South Korea.
authors.forename: Suresh
authors.initials: S
authors.lastname: Ramakrishna
authors.affiliation: Uppsala University, 75236 Uppsala, Sweden.
authors.forename: Himanshu
authors.initials: H
authors.lastname: Chauhan
authors.affiliation: Department of Physiology, School of Medicine, Kangwon National University, Chuncheon, 24341 South Korea.
authors.forename: Won Sun
authors.initials: WS
authors.lastname: Park
authors.affiliation: Department of Internal Medicine, School of Medicine, Kangwon National University, 1 Kangwondaehak-gil, Chuncheon-si, Gangwon-do 24341 South Korea.|Stem Cell Institute, Kangwon National University, Chuncheon, 24341 South Korea.
authors.forename: Seok-Ho
authors.initials: SH
authors.lastname: Hong
authors.affiliation: Department of Biomedical Science, Graduate School of Biomedical Science and Engineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul, 04763 South Korea.|College of Medicine, Hanyang University, Seoul, South Korea.
authors.forename: Kye-Seong
authors.initials: KS
authors.lastname: Kim
keywords: Embryonic stem cells; Pluripotency; Reprogramming; Self-renewal; miRNAs