title: Potential of phosphoric acid-catalyzed pretreatment and subsequent enzymatic hydrolysis for biosugar production from Gracilaria verrucosa.
abstract: This study combined phosphoric acid-catalyzed pretreatment and enzymatic hydrolysis to produce biosugars from Gracilaria verrucosa as a potential renewable resource for bioenergy applications. We optimized phosphoric acid-catalyzed pretreatment conditions to 1:10 solid-to-liquid ratio, 1.5 % phosphoric acid, 140 °C, and 60 min reaction time, producing a 32.52 ± 0.06 % total reducing sugar (TRS) yield. By subsequent enzymatic hydrolysis, a 68.61 ± 0.90 % TRS yield was achieved. These results demonstrate the potential of phosphoric acid to produce biosugars for biofuel and biochemical production applications.
authors.affiliation: Department of Biotechnology, Pukyong National University, Busan, 48513, Republic of Korea.
authors.forename: Oh-Min
authors.initials: OM
authors.lastname: Kwon
authors.affiliation: Department of Biotechnology, Pukyong National University, Busan, 48513, Republic of Korea.
authors.forename: Sung-Koo
authors.initials: SK
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, Pukyong National University, Busan, 48513, Republic of Korea. gtjeong@pknu.ac.kr.
authors.forename: Gwi-Taek
authors.initials: GT
authors.lastname: Jeong
mesh_terms: D018533:Biomass; D002241:Carbohydrates / Q000096:biosynthesis*; D002384:Catalysis; D044724:Gracilaria / Q000378:metabolism*; D006868:Hydrolysis; D010756:Phosphoric Acids / Q000737:chemistry*; D013696:Temperature
chemical_list: D002241:Carbohydrates; D010756:Phosphoric Acids; C030242:phosphoric acid
keywords: Biosugars; Enzymatic hydrolysis; Gracilaria verrucosa; Phosphoric acid-catalyzed pretreatment