title: The accuracy of the anti-phospholipase A2 receptor antibody in the diagnosis of idiopathic membranous nephropathy: a comparison of different cutoff values as measured by the ELISA method.
abstract: OBJECTIVE
  Recent studies have revealed that anti-phospholipase A2 receptor antibodies (aPLA2R-ABs) may play a role in the diagnosis of idiopathic membranous nephropathy (IMN). We will investigate the application of an aPLA2R-AB with different cutoff values for diagnosing IMN.
  
  
  METHODS
  From August 2014 to December 2014, patients with proteinuria greater than 1 g/day were screened at the First Affiliated Hospital of Zhengzhou University. Patients were divided into the IMN and non-IMN groups based on the results of renal biopsy. The sensitivity, specificity, and receiver operating characteristic (ROC) curve of aPLA2R-AB for diagnosing IMN were analyzed.
  
  
  RESULTS
  A total of 229 patients (113 males, average age of 45.3 ± 15.8 years) were enrolled in this study, and 118 patients were diagnosed with IMN. The sensitivity/specificity of aPLA2R-AB in the diagnosis of IMN was 65.3/97.3 %, 60.2/97.3 %, and 45.8/97.3 % for the different cutoff values (14, 20, and 40 RU/ml). The area of the ROC curve was 0.87.
  
  
  CONCLUSIONS
  Anti-PLA2R-AB with a cutoff value of 14 RU/ml based on the ELISA method plays an important role in the diagnosis of IMN.
authors.affiliation: The Nephrology Center of the First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe Eastern Road, 6th Floor of No. 7th Building, Erqi District, Zhengzhou, 450052, Henan, China.|Zhengzhou University Institute of Nephrology, Zhengzhou, China.
authors.forename: Yanna
authors.initials: Y
authors.lastname: Dou
authors.affiliation: The Nephrology Center of the First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe Eastern Road, 6th Floor of No. 7th Building, Erqi District, Zhengzhou, 450052, Henan, China.|Zhengzhou University Institute of Nephrology, Zhengzhou, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: The Nephrology Center of the First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe Eastern Road, 6th Floor of No. 7th Building, Erqi District, Zhengzhou, 450052, Henan, China. liudong030@163.com.|Zhengzhou University Institute of Nephrology, Zhengzhou, China. liudong030@163.com.
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.affiliation: The Nephrology Center of the First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe Eastern Road, 6th Floor of No. 7th Building, Erqi District, Zhengzhou, 450052, Henan, China.|Zhengzhou University Institute of Nephrology, Zhengzhou, China.|The Key Laboratory of CKD and Renal Pathology of Henan Province, Zhengzhou, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Wang
authors.affiliation: The Nephrology Center of the First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe Eastern Road, 6th Floor of No. 7th Building, Erqi District, Zhengzhou, 450052, Henan, China.|Zhengzhou University Institute of Nephrology, Zhengzhou, China.|The Key Laboratory of CKD and Renal Pathology of Henan Province, Zhengzhou, China.
authors.forename: Songxia
authors.initials: S
authors.lastname: Quan
authors.affiliation: The Nephrology Center of the First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe Eastern Road, 6th Floor of No. 7th Building, Erqi District, Zhengzhou, 450052, Henan, China.|Zhengzhou University Institute of Nephrology, Zhengzhou, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Ma
authors.affiliation: The Nephrology Center of the First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe Eastern Road, 6th Floor of No. 7th Building, Erqi District, Zhengzhou, 450052, Henan, China.|Zhengzhou University Institute of Nephrology, Zhengzhou, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xiao
authors.affiliation: The Nephrology Center of the First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe Eastern Road, 6th Floor of No. 7th Building, Erqi District, Zhengzhou, 450052, Henan, China.|Zhengzhou University Institute of Nephrology, Zhengzhou, China.
authors.forename: Genyang
authors.initials: G
authors.lastname: Cheng
authors.affiliation: The Nephrology Center of the First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe Eastern Road, 6th Floor of No. 7th Building, Erqi District, Zhengzhou, 450052, Henan, China.|Zhengzhou University Institute of Nephrology, Zhengzhou, China.
authors.forename: Zhangsuo
authors.initials: Z
authors.lastname: Liu
authors.affiliation: The Nephrology Center of the First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe Eastern Road, 6th Floor of No. 7th Building, Erqi District, Zhengzhou, 450052, Henan, China. zzzdoctor@139.com.|Zhengzhou University Institute of Nephrology, Zhengzhou, China. zzzdoctor@139.com.
authors.forename: Zhanzheng
authors.initials: Z
authors.lastname: Zhao
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000097:blood*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015433:Glomerulonephritis, Membranous / Q000097:blood* / Q000175:diagnosis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D012372:ROC Curve; D054507:Receptors, Phospholipase A2 / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D054507:Receptors, Phospholipase A2
keywords: Anti-PLA2R antibody; ELISA method; Idiopathic membranous nephropathy; Sensitivity; Specificity