title: Preparation and characterization of PLGA-β-CD polymeric nanoparticles containing methotrexate and evaluation of their effects on T47D cell line.
abstract: Among all cancers that affect women, breast cancer has most mortality rate. It is essential to attain more safe and efficient anticancer drugs. Recent advances in medical nanotechnology and biotechnology have caused in novel improvements in breast and other cancer drug delivery. Methotrexate is an anticancer drug that prevents the dihydrofolate reductase enzyme, which inhibits in the formation of DNA, RNA and proteins which have poor water-solubility. For enhancing the solubility and stability of drugs in delivery systems, we used methotrexate-loaded PLGA- beta-cyclodextrin nanoparticles. The PLGA- beta-cyclodextrin nanoparticles were synthesized by a double emulsion method and characterized with FT-IR and SEM. T47D breast cancer cell lines were treated with equal concentrations of methotrexate-loaded PLGA- beta-cyclodextrin nanoparticles and free methotrexate. MTT assay confirmed that methotrexate-loaded PLGA- beta-cyclodextrin nanoparticles enhanced cytotoxicity and drug delivery in T47D breast cancer cells. These results indicate that encapsulated drugs could be effective in controlled drug release for a sustained period would serve the purpose for long-term treatment of many diseases such as breast cancer.
authors.affiliation: a Drug Applied Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.|b Department of Medical Nanotechnology, Faculty of Advanced Medical Science , Tabriz University of Medical Sciences , Tabriz , Iran.|c Department of Medical Virology, Faculty of Medicine , Hamadan University of Medical Sciences , Hamadan , Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Gorjikhah
authors.affiliation: c Department of Medical Virology, Faculty of Medicine , Hamadan University of Medical Sciences , Hamadan , Iran.
authors.forename: Farid
authors.initials: F
authors.lastname: Azizi Jalalian
authors.affiliation: a Drug Applied Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.|b Department of Medical Nanotechnology, Faculty of Advanced Medical Science , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Roya
authors.initials: R
authors.lastname: Salehi
authors.affiliation: d Chemical Injuries Research Center , Baqiyatallah University of Medical Sciences , Tehran , Iran.
authors.forename: Yunes
authors.initials: Y
authors.lastname: Panahi
authors.affiliation: e Laboratory of Biochemistry, Department of Biology, Faculty of Natural Sciences , University of Tabriz , Tabriz , Iran.
authors.forename: Arash
authors.initials: A
authors.lastname: Hasanzadeh
authors.affiliation: f Department of Medical Biotechnology, Faculty of Advanced Medical Sciences , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Effat
authors.initials: E
authors.lastname: Alizadeh
authors.affiliation: a Drug Applied Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.|b Department of Medical Nanotechnology, Faculty of Advanced Medical Science , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Akbarzadeh
authors.affiliation: a Drug Applied Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.|b Department of Medical Nanotechnology, Faculty of Advanced Medical Science , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Soodabeh
authors.initials: S
authors.lastname: Davaran
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D001692:Biological Transport; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers; D004339:Drug Compounding; D065546:Drug Liberation; D005260:Female; D006801:Humans; D007700:Kinetics; D019344:Lactic Acid / Q000737:chemistry* / Q000378:metabolism; D061986:MCF-7 Cells; D008727:Methotrexate / Q000737:chemistry / Q000494:pharmacology*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry* / Q000378:metabolism; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D047392:beta-Cyclodextrins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D047392:beta-Cyclodextrins; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D008727:Methotrexate
keywords: Breast cancer; PLGA; cyclodextrin; methotrexate