title: Bubble electrodeposition of gold porous nanocorals for the enzymatic and non-enzymatic detection of glucose.
abstract: Au nanocorals are grown on gold screen-printed electrodes (SPEs) by using a novel and simple one-step electrodeposition process. Scanning electron microscopy was used for the morphological characterization. The devices were assembled on a three-electrode SPE system, which is flexible and mass producible. The electroactive surface area, determined by cyclic voltammetry in sulphuric acid, was found to be 0.07±0.01cm(2) and 35.3±2.7cm(2) for bare Au and nanocoral Au, respectively. The nanocoral modified SPEs were used to develop an enzymatic glucose biosensor based on H2O2 detection. Au nanocoral electrodes showed a higher sensitivity of 48.3±0.9μA/(mMcm(2)) at +0.45V vs Ag|AgCl compared to a value of 24.6±1.3μA/(mMcm(2)) at +0.70V vs Ag|AgCl obtained with bare Au electrodes. However, the modified electrodes have indeed proven to be extremely powerful for the direct detection of glucose with a non-enzymatic approach. The results confirmed a clear peak observed by using nanocoral Au electrode even in the presence of chloride ions at physiological concentration. Amperometric study carried out at +0.15V vs Ag|AgCl in the presence of 0.12M NaCl showed a linear range for glucose between 0.1 and 13mM.
authors.affiliation: Laboratory of Integrated Systems, EPFL - École Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland; Biosensors Laboratory, Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro, 5-00185 Roma, Italy. Electronic address: gabriella.sanzo@uniroma1.it.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Sanzó
authors.affiliation: Laboratory of Integrated Systems, EPFL - École Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.
authors.forename: Irene
authors.initials: I
authors.lastname: Taurino
authors.affiliation: Biosensors Laboratory, Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro, 5-00185 Roma, Italy. Electronic address: riccarda.antiochia@uniroma1.it.
authors.forename: Riccarda
authors.initials: R
authors.lastname: Antiochia
authors.affiliation: Department of Analytical Chemistry/Biochemistry, P.O. Box 124, 221 00 Lund, Sweden.
authors.forename: Lo
authors.initials: L
authors.lastname: Gorton
authors.affiliation: Biosensors Laboratory, Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro, 5-00185 Roma, Italy.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Favero
authors.affiliation: Biosensors Laboratory, Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro, 5-00185 Roma, Italy.
authors.forename: Franco
authors.initials: F
authors.lastname: Mazzei
authors.affiliation: Laboratory of Integrated Systems, EPFL - École Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.
authors.forename: Giovanni
authors.initials: G
authors.lastname: De Micheli
authors.affiliation: Laboratory of Integrated Systems, EPFL - École Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.
authors.forename: Sandro
authors.initials: S
authors.lastname: Carrara
mesh_terms: D015374:Biosensing Techniques; D004563:Electrochemistry; D004566:Electrodes; D004595:Electroplating / Q000379:methods*; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D016062:Porosity
chemical_list: D006046:Gold; D005949:Glucose Oxidase; D005947:Glucose
keywords: Electroactive surface area; Glucose oxidase; Glucose oxidation; Gold nanocorals; Hydrogen template electrodeposition; Potassium ferricyanide