title: Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells.
abstract: Nasal epithelial cells (NECs) are the part of the airways that respond to air pollutants and are the first cells infected with respiratory viruses. They are also involved in many airway diseases through their innate immune response and interaction with immune and airway stromal cells. NECs are of particular interest for studies in children due to their accessibility during clinical visits. Human induced pluripotent stem cells (iPSCs) have been generated from multiple cell types and are a powerful tool for modeling human development and disease, as well as for their potential applications in regenerative medicine. This is the first protocol to lay out methods for successful generation of iPSCs from NECs derived from pediatric participants for research purposes. It describes how to obtain nasal epithelial cells from children, how to generate primary NEC cultures from these samples, and how to reprogram primary NECs into well-characterized iPSCs. Nasal mucosa samples are useful in epidemiological studies related to the effects of air pollution in children, and provide an important tool for studying airway disease. Primary nasal cells and iPSCs derived from them can be a tool for providing unlimited material for patient-specific research in diverse areas of airway epithelial biology, including asthma and COPD research.
authors.affiliation: Pyrosequencing Core, Cincinnati Children's Hospital.
authors.forename: Ashley
authors.initials: A
authors.lastname: Ulm
authors.affiliation: Division of Developmental Biology, Cincinnati Children's Hospital.
authors.forename: Christopher N
authors.initials: CN
authors.lastname: Mayhew
authors.affiliation: Division of Pulmonary Medicine, Seattle Children's Hospital.
authors.forename: Jason
authors.initials: J
authors.lastname: Debley
authors.affiliation: Division of Asthma Research, Cincinnati Children's Hospital.
authors.forename: Gurjit K
authors.initials: GK
authors.lastname: Khurana Hershey
authors.affiliation: Pyrosequencing Core, Cincinnati Children's Hospital; Division of Asthma Research, Cincinnati Children's Hospital; Hong.Ji@cchmc.org.
authors.forename: Hong
authors.initials: H
authors.lastname: Ji
mesh_terms: D065150:Cellular Reprogramming; D002648:Child; D004847:Epithelial Cells / Q000166:cytology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D009297:Nasal Mucosa / Q000166:cytology*; D061251:Primary Cell Culture / Q000379:methods*