title: Involvement of miR-454 overexpression in the poor prognosis of hepatocellular carcinoma.
abstract: OBJECTIVE
  Many studies informed that microRNAs (miRNAs) could function as diagnostic and prognostic indicators in several cancers. The prognostic value of miR-454 in hepatocellular carcinoma has not been investigated.
  
  
  METHODS
  A total of 265 patients with HCC were obtained in this retrospective study between June 2009 and July 2014. qPCR was conducted to evaluate the expressed amount of the miR-454. The Kaplan-Meier method was conducted to explore the survival status of HCC patients. The log-rank test was used to analyze differences in survival rates.
  
  
  RESULTS
  The expression of miR-454 was significantly upregulated in HCC tissues compared with adjacent non-cancerous tissues (p < 0.001). High levels of miR-454 in HCC tissues were correlated with a low 5-year overall survival (OS) (p < 0.001). Moreover, patients with high miR-454 expression had decreased disease-free survival (DFS) (p < 0.001). Furthermore, multivariate analysis showed that up-regulation of miR-454 was an independent prognostic factor for both 5-year OS (p = 0.013) and 5-year DFS (p = 0.008).
  
  
  CONCLUSIONS
  We firstly prove that expression of miR-454 may be a novel and valuable prognostic factor in HCC.
authors.affiliation: Departmemt of Oncology, PLA General Hospital, Peking, China. zhen0102y@sina.com.
authors.forename: L
authors.initials: L
authors.lastname: Zhou
authors.forename: Y-M
authors.initials: YM
authors.lastname: Qu
authors.forename: X-M
authors.initials: XM
authors.lastname: Zhao
authors.forename: Z-D
authors.initials: ZD
authors.lastname: Yue
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D015854:Up-Regulation
chemical_list: C583712:MIRN454 microRNA, human; D035683:MicroRNAs