title: An evaluation of the engraftment and the blood flow of porcine skin autografts inactivated by high hydrostatic pressure.
abstract: We previously reported that exposure to a high hydrostatic pressure (HHP) of 200 MPa could completely inactivate porcine skin without damaging the extracellular matrix. In this study, we used an autologous porcine skin graft model and explored whether the skin inactivated by HHP could be engrafted without inflammation to the residual cellular components. Twenty-one full-thickness skin grafts of 1.5 × 1.5 cm in size were prepared from a minipig (n = 2). Grafts were either nonpressurized or pressurized to 100, 150, 200, 300, 500, or 1000 MPa (n = 3) and randomly implanted on the fascia and removed at 1 and 4 weeks after grafting. All grafts showed complete engraftment at the macroscopic level and microcirculation was detected by a full-field laser speckle perfusion imager. The epidermis was removed and skin appendages were not observed in the grafts pressurized to more than 200 MPa. Azan and Elastica van Gieson staining showed no sign of dermal collagen fiber degeneration, while elastin fibers were observed. The fibroblasts and capillaries were observed to have infiltrated to dermis in all groups without severe inflammation. In conclusion, we showed that skin inactivated by HHP up to 1000 MPa could be engrafted successfully without removing cellular remnants. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1091-1101, 2017.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Kansai Medical University, Hirakata City, Osaka, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Morimoto
authors.affiliation: Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Suita City, Osaka, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Mahara
authors.affiliation: Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, Kyoto City, Kyoto, Japan.
authors.forename: Chizuru
authors.initials: C
authors.lastname: Jinno
authors.affiliation: Department of Biomedical Engineering, Osaka Institute of Technology, Osaka City, Osaka, Japan.
authors.forename: Mami
authors.initials: M
authors.lastname: Ogawa
authors.affiliation: Department of Plastic and Reconstructive Surgery, Kansai Medical University, Hirakata City, Osaka, Japan.
authors.forename: Natsuko
authors.initials: N
authors.lastname: Kakudo
authors.affiliation: Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, Kyoto City, Kyoto, Japan.
authors.forename: Shigehiko
authors.initials: S
authors.lastname: Suzuki
authors.affiliation: Department of Plastic and Reconstructive Surgery, Kansai Medical University, Hirakata City, Osaka, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kusumoto
authors.affiliation: Department of Biomedical Engineering, Osaka Institute of Technology, Osaka City, Osaka, Japan.
authors.forename: Toshia
authors.initials: T
authors.lastname: Fujisato
authors.affiliation: Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Suita City, Osaka, Japan.
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Yamaoka
mesh_terms: D000818:Animals; D064592:Autografts; D002196:Capillaries / Q000378:metabolism / Q000473:pathology; D003094:Collagen / Q000378:metabolism; D020405:Dermis / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D004549:Elastin / Q000378:metabolism; D006082:Graft Enhancement, Immunologic / Q000379:methods*; D006874:Hydrostatic Pressure; D016038:Skin Transplantation; D013552:Swine
chemical_list: D003094:Collagen; D004549:Elastin
keywords: high hydrostatic pressure; inactivation; skin graft