title: Extracellular trap formation in kuruma shrimp (Marsupenaeus japonicus) hemocytes is coupled with c-type lysozyme.
abstract: In invertebrates, hemocytes play an important role in immune responses. Recently, a novel form of innate immune mechanism called extracellular traps (ETs) was identified in shrimps, where DNA and antimicrobial peptides form complex structure to entrap the invading microbes. In this study, we detected the formation of ETs from hemocytes of kuruma shrimp in response to various stimulations, including phorbol myristate acetate (PMA), lipopolysaccharide (LPS), peptidoglycan (PGN) and Escherichia coli. E. coli cells were also found to be trapped by ET fibers. Fluorescence imaging revealed that c-type lysozyme proteins were released around the ET complex after E. coli stimulation, suggesting the presence of a coupled antimicrobial immune response involving ET formation and AMP release.
authors.affiliation: Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Tokyo, Japan.
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Koiwai
authors.affiliation: Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Tokyo, Japan.
authors.forename: Rod Russel R
authors.initials: RR
authors.lastname: Alenton
authors.affiliation: Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Tokyo, Japan.
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Kondo
authors.affiliation: Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Tokyo, Japan. Electronic address: hirono@kaiyodai.ac.jp.
authors.forename: Ikuo
authors.initials: I
authors.lastname: Hirono
mesh_terms: D000818:Animals; D060829:Arthropod Proteins; D004926:Escherichia coli / Q000502:physiology; D065206:Extracellular Traps / Q000201:enzymology; D006434:Hemocytes / Q000187:drug effects / Q000201:enzymology / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D009113:Muramidase / Q000235:genetics / Q000378:metabolism*; D033561:Penaeidae / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology*; D010457:Peptidoglycan / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D060829:Arthropod Proteins; D008070:Lipopolysaccharides; D010457:Peptidoglycan; D009113:Muramidase; D013755:Tetradecanoylphorbol Acetate
keywords: Extracellular traps (ETs); Hemocytes; Shrimp; c-type lysozyme