title: Improvement of l-lactic acid productivity from sweet sorghum juice by repeated batch fermentation coupled with membrane separation.
abstract: In order to efficiently produce l-lactic acid from non-food feedstocks, sweet sorghum juice (SSJ), which is rich of fermentable sugars, was directly used for l-lactic acid fermentation by Lactobacillus rhamnosus LA-04-1. A membrane integrated repeated batch fermentation (MIRB) was developed for productivity improvement. High-cell-density fermentation was achieved with a final cell density (OD620) of 42.3, and the CCR effect was overcomed. When SSJ (6.77gL(-1) glucose, 4.51gL(-1) fructose and 50.46gL(-1) sucrose) was used as carbon source in MIRB process, l-lactic acid productivity was increased significantly from 1.45gL(-1)h(-1) (batch 1) to 17.55gL(-1)h(-1) (batch 6). This process introduces an effective way to produce l-lactic acid from SSJ.
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Meng
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Di
authors.initials: D
authors.lastname: Cai
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Peiyong
authors.initials: P
authors.lastname: Qin
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address: wangzheng@mail.buct.edu.cn.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Tianwei
authors.initials: T
authors.lastname: Tan
mesh_terms: D019149:Bioreactors; D005285:Fermentation; D019344:Lactic Acid / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D052201:Lacticaseibacillus rhamnosus; D008567:Membranes, Artificial; D045868:Sorghum / Q000737:chemistry / Q000378:metabolism
chemical_list: D008567:Membranes, Artificial; D019344:Lactic Acid
keywords: Lactobacillus rhamnosus; Repeated batch fermentation; Sweet sorghum juice; l-Lactic acid