title: Intracellular interactions of electrostatically mediated layer-by-layer assembled polyelectrolytes based sorafenib nanoparticles in oral cancer cells.
abstract: In this paper, we report the preparation of LbL-nanoSraf (100-300nm) comprising of layer-by-layer (LbL) assembled polyelectrolytes dextran-sulfate/poly-l-arginine, with a multikinase inhibitor sorafenib (Sraf) encapsulated calcium carbonate (CaCO3) nanoparticles for oral cancer therapy in vitro. The zeta potential of LbL-nanoSraf exhibited a negative charge of the polyanionic dextran sulfate, which alternated with a positive charge of polycationic poly-l-arginine indicating a successful LbL assembly of the two polyelectrolyte bilayers on the CaCO3 nanoparticles. The LbL-nanoSraf exhibited an encapsulation efficiency of 61±4%. The LbL-nanoSraf was characterized using field-emission gun scanning electron microscopy, X-ray powder diffraction, atomic force microscopy and confocal laser scanning microscopy. Confocal laser scanning microscopy, flow cytometry and transmission electron microscopic investigations showed the internalization of LbL-nanoSraf in human oral cancer (KB) cells. The LbL-nanoSraf exhibited more potent antiproliferative, apoptotic and antimigratory activities in KB cells than the free drug Sraf. The findings could promote the application of nano-sized LbL assembled polyelectrolytes for the delivery of Raf-kinase inhibitors and provide mechanistic insights for oral cancer therapy.
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai 400076, India. Electronic address: drradhikapoojari@gmail.com.
authors.forename: Radhika
authors.initials: R
authors.lastname: Poojari
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai 400076, India.
authors.forename: Sudarshan
authors.initials: S
authors.lastname: Kini
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai 400076, India.
authors.forename: Rohit
authors.initials: R
authors.lastname: Srivastava
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai 400076, India. Electronic address: panda@iitb.ac.in.
authors.forename: Dulal
authors.initials: D
authors.lastname: Panda
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002119:Calcium Carbonate / Q000737:chemistry; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D016264:Dextran Sulfate / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry* / Q000494:pharmacology; D004339:Drug Compounding; D065546:Drug Liberation; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology; D006801:Humans; D007700:Kinetics; D009061:Mouth Mucosa / Q000187:drug effects / Q000473:pathology; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009536:Niacinamide / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D010455:Peptides / Q000737:chemistry; D010671:Phenylurea Compounds / Q000737:chemistry / Q000494:pharmacology*; D000071228:Polyelectrolytes / Q000737:chemistry* / Q000494:pharmacology; D047428:Protein Kinase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D000077157:Sorafenib; D055672:Static Electricity
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D010455:Peptides; D010671:Phenylurea Compounds; D000071228:Polyelectrolytes; D047428:Protein Kinase Inhibitors; C015462:polyarginine; D009536:Niacinamide; D016264:Dextran Sulfate; D000077157:Sorafenib; D002119:Calcium Carbonate
keywords: Calcium carbonate nanoparticles; Kinase inhibitor; Layer-by-layer assembled polyelectrolytes; Oral cancer cells; Sorafenib