title: Inducible DamID systems for genomic mapping of chromatin proteins in Drosophila.
abstract: Dam identification (DamID) is a powerful technique to generate genome-wide maps of chromatin protein binding. Due to its high sensitivity, it is particularly suited to study the genome interactions of chromatin proteins in small tissue samples in model organisms such as Drosophila Here, we report an intein-based approach to tune the expression level of Dam and Dam-fusion proteins in Drosophila by addition of a ligand to fly food. This helps to suppress possible toxic effects of Dam. In addition, we describe a strategy for genetically controlled expression of Dam in a specific cell type in complex tissues. We demonstrate the utility of the latter by generating a glia-specific map of Polycomb in small samples of brain tissue. These new DamID tools will be valuable for the mapping of binding patterns of chromatin proteins in Drosophila tissues and especially in cell lineages.
authors.affiliation: Division of Gene Regulation, Netherlands Cancer Institute, 1066 CX Amsterdam, the Netherlands Institute of Molecular and Cellular Biology SB RAS, Novosibirsk 630090, Russia a.pindyurin@mcb.nsc.ru.
authors.forename: Alexey V
authors.initials: AV
authors.lastname: Pindyurin
authors.affiliation: Division of Gene Regulation, Netherlands Cancer Institute, 1066 CX Amsterdam, the Netherlands.
authors.forename: Ludo
authors.initials: L
authors.lastname: Pagie
authors.affiliation: Institute of Molecular and Cellular Biology SB RAS, Novosibirsk 630090, Russia.
authors.forename: Elena N
authors.initials: EN
authors.lastname: Kozhevnikova
authors.affiliation: Division of Gene Regulation, Netherlands Cancer Institute, 1066 CX Amsterdam, the Netherlands.
authors.forename: Joris
authors.initials: J
authors.lastname: van Arensbergen
authors.affiliation: Division of Gene Regulation, Netherlands Cancer Institute, 1066 CX Amsterdam, the Netherlands b.v.steensel@nki.nl.
authors.forename: Bas
authors.initials: B
authors.lastname: van Steensel
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics; D001665:Binding Sites; D019070:Cell Lineage / Q000235:genetics; D002843:Chromatin / Q000235:genetics*; D002874:Chromosome Mapping; D019175:DNA Methylation / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D005786:Gene Expression Regulation; D008024:Ligands; D009457:Neuroglia / Q000378:metabolism; D009928:Organ Specificity; D011485:Protein Binding; D014176:Protein Biosynthesis / Q000235:genetics; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D002843:Chromatin; D008024:Ligands; C058693:Dam methyltransferase; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)