title: Pharmacological Investigation of the Wound Healing Activity of Cestrum nocturnum (L.) Ointment in Wistar Albino Rats.
abstract: Objectives. The present study was aimed at investigating the wound healing effect of ethanolic extract of Cestrum nocturnum (L.) leaves (EECN) using excision and incision wound model. Methods. Wistar albino rats were divided into five groups each consisting of six animals; group I (left untreated) considered as control, group II (ointment base treated) considered as negative control, group III treated with 5% (w/w) povidone iodine ointment (Intadine USP), which served as standard, group IV treated with EECN 2% (w/w) ointment, and group V treated with EECN 5% (w/w) ointment were considered as test groups. All the treatments were given once daily. The wound healing effect was assessed by percentage wound contraction, epithelialization period, and histoarchitecture studies in excision wound model while breaking strength and hydroxyproline content in the incision wound model. Result. Different concentration of EECN (2% and 5% w/w) ointment promoted the wound healing activity significantly in both the models studied. The high rate of wound contraction (P < 0.001), decrease in the period for epithelialization (P < 0.01), high skin breaking strength (P < 0.001), and elevated hydroxyproline content were observed in animal treated with EECN ointments when compared to the control and negative control group of animals. Histopathological studies of the EECN ointments treated groups also revealed the effectiveness in improved wound healing. Conclusions. Ethanolic extract of Cestrum nocturnum (EECN) leaves possesses a concentration dependent wound healing effect.
authors.affiliation: Department of Pharmacology, Truba Institute of Pharmacy, Karond Gandhi Nagar, Bhopal, Madhya Pradesh 462038, India.
authors.forename: Hemant Kumar
authors.initials: HK
authors.lastname: Nagar
authors.affiliation: Department of Pharmacology, Sapience Bioanalytical Research Lab, Bhopal, Madhya Pradesh 462021, India.
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Srivastava
authors.affiliation: Faculty of Pharmacy, Moradabad Educational Trust Group of Institutions, Moradabad, Uttar Pradesh 244001, India.
authors.forename: Rajnish
authors.initials: R
authors.lastname: Srivastava
authors.affiliation: Department of Pharmacology, Truba Institute of Pharmacy, Karond Gandhi Nagar, Bhopal, Madhya Pradesh 462038, India.
authors.forename: Madan Lal
authors.initials: ML
authors.lastname: Kurmi
authors.affiliation: Department of Pharmacology, Truba Institute of Pharmacy, Karond Gandhi Nagar, Bhopal, Madhya Pradesh 462038, India.
authors.forename: Harinarayan Singh
authors.initials: HS
authors.lastname: Chandel
authors.affiliation: Bhupal Nobles' College of Pharmacy, Udaipur, Rajasthan 313002, India.
authors.forename: Mahendra Singh
authors.initials: MS
authors.lastname: Ranawat