title: Preventive effect of toothpastes with MMP inhibitors on human dentine erosion and abrasion in vitro.
abstract: The use of gels and mouthrinses with MMP inhibitors (chlorhexidine, and green tea extract) was shown to prevent erosive wear. The aim of this study was to analyze the protective effect of toothpastes containing MMP inhibitors on dentine loss induced by erosion in vitro.
  
  
  METHODS
  Five groups each containing 12 specimens of human root dentine were prepared. The specimens were subjected to 1 min erosion by immersion in a cola drink, 4 times a day, for 5 d. Each day, after the first and last erosive challenges, the specimens were brushed for 15 s with a slurry of dentifrice and water (1:3) containing placebo, 1,100 ppm fluoride, 0.61% green tea extract, 0.12% chlorhexidine or 0.004% chlorhexidine (commercial toothpaste). Between the acid challenges, the specimens were stored in artificial saliva with remineralizing potential until the next treatment. Dentine loss was determined using profilometry. Data were analyzed using one-way ANOVA after log transform (p<0.05).
  
  
  RESULTS
  The mean wear values (μm) were as follows: placebo 1.83±0.53; 0.61% green tea extract 1.00±0.21; fluoride 1.27±0.43; 0.12% chlorhexidine 1.19±0.30; and 0.004% chlorhexidine 1.22±0.46. There was a significant difference in wear between placebo and all the treatment toothpastes, which did not differ from each other.
  
  
  CONCLUSIONS
  The results suggest that toothpastes containing MMP inhibitors are as effective as those based on NaF in preventing dentine erosion and abrasion.
authors.affiliation: Departamento de Ciências Biológicas, Faculdade de Odontologia de Bauru, Universidade de São Paulo, Bauru, SP, Brasil.
authors.forename: Angelica Reis
authors.initials: AR
authors.lastname: Hannas
authors.affiliation: Departamento de Odontologia, Universidade do Sagrado Coração, Bauru, SP, Brasil.
authors.forename: Melissa Thiemi
authors.initials: MT
authors.lastname: Kato
authors.affiliation: Departamento de Odontopediatria, Universidade do Sagrado Coração, Bauru, SP, Brasil.
authors.forename: Cristiane de Almeida Baldini
authors.initials: Cde A
authors.lastname: Cardoso
authors.affiliation: Departamento de Ciências Biológicas, Faculdade de Odontologia de Bauru, Universidade de São Paulo, Bauru, SP, Brasil.
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Magalhães
authors.affiliation: Departamento de Dentística, Faculdade de Odontologia de Bauru, Universidade de São Paulo, Bauru, SP, Brasil.
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Pereira
authors.affiliation: Medical Research Center, Oulu University Hospital, University of Oulu, Oulu, Finland.
authors.forename: Leo
authors.initials: L
authors.lastname: Tjäderhane
authors.affiliation: Departamento de Ciências Biológicas, Faculdade de Odontologia de Bauru, Universidade de São Paulo, Bauru, SP, Brasil.
authors.forename: Marília Afonso Rabelo
authors.initials: MA
authors.lastname: Buzalaf
mesh_terms: D000704:Analysis of Variance; D002253:Carbonated Beverages; D002710:Chlorhexidine / Q000737:chemistry; D003804:Dentin / Q000187:drug effects*; D006801:Humans; D008422:Materials Testing; D061965:Matrix Metalloproteinase Inhibitors / Q000737:chemistry*; D011897:Random Allocation; D012464:Saliva, Artificial / Q000737:chemistry; D013499:Surface Properties / Q000187:drug effects; D013997:Time Factors; D014072:Tooth Abrasion / Q000517:prevention & control*; D014077:Tooth Erosion / Q000517:prevention & control*; D014099:Toothbrushing; D014100:Toothpastes / Q000737:chemistry*
chemical_list: D061965:Matrix Metalloproteinase Inhibitors; D012464:Saliva, Artificial; D014100:Toothpastes; D002710:Chlorhexidine