title: Comparative proteomic and bioinformatic analysis of Theileria luwenshuni and Theileria uilenbergi.
abstract: Theileria is an obligatory intraerythrocytic protozoan parasite that causes economic losses to the cattle, sheep and goats industry. However, very little information is available on the genomes, transcriptomes, and proteomes of the ovine parasites, Theileria luwenshuni and Theileria uilenbergi. Differences in protein expression between these species were investigated to better understand their biology. Parasites were digested with trypsin, and the resulting peptides labeled with isobaric tags for relative and absolute quantification, followed by LC-MS/MS. More than 670 proteins, classified into categories primarily related to cellular process (29.78%), metabolic process (28.80%), localization (5.22%) and biological regulation (5.00%), were identified. Seventy-one proteins were differentially expressed; T. luwenshuni had 39 proteins more highly expressed than in T. uilenbergi, whereas T. uilenbergi had 32 that were more highly expressed. Several proteins related to parasite virulence and invasion (cysteine proteinase, histone deacetylase, pyruvate kinase, small nuclear ribonucleoprotein and orotate phosphoribosyltransferase) were differentially expressed. Real-time quantitative PCR validated protein expression changes at the transcript level. This is the first report on protein expression for the two most economically important Theileria species in China, and our findings may provide novel opportunities for ovine and caprine theileriosis control.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, 1 Xujiaping, Lanzhou, Gansu 730046, PR China; Key Laboratory of Veterinary Parasitology of Gansu Province, 1 Xujiaping, Lanzhou, Gansu 730046, PR China; Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, 1 Xujiaping, Lanzhou, Gansu 730046, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, 1 Xujiaping, Lanzhou, Gansu 730046, PR China; Key Laboratory of Veterinary Parasitology of Gansu Province, 1 Xujiaping, Lanzhou, Gansu 730046, PR China; Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, 1 Xujiaping, Lanzhou, Gansu 730046, PR China. Electronic address: youquan-li@163.com.
authors.forename: Youquan
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, 1 Xujiaping, Lanzhou, Gansu 730046, PR China; Key Laboratory of Veterinary Parasitology of Gansu Province, 1 Xujiaping, Lanzhou, Gansu 730046, PR China; Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, 1 Xujiaping, Lanzhou, Gansu 730046, PR China.
authors.forename: Ze
authors.initials: Z
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, 1 Xujiaping, Lanzhou, Gansu 730046, PR China; Key Laboratory of Veterinary Parasitology of Gansu Province, 1 Xujiaping, Lanzhou, Gansu 730046, PR China; Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, 1 Xujiaping, Lanzhou, Gansu 730046, PR China.
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, 1 Xujiaping, Lanzhou, Gansu 730046, PR China; Key Laboratory of Veterinary Parasitology of Gansu Province, 1 Xujiaping, Lanzhou, Gansu 730046, PR China; Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, 1 Xujiaping, Lanzhou, Gansu 730046, PR China.
authors.forename: Qiaoyun
authors.initials: Q
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, 1 Xujiaping, Lanzhou, Gansu 730046, PR China; Key Laboratory of Veterinary Parasitology of Gansu Province, 1 Xujiaping, Lanzhou, Gansu 730046, PR China; Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, 1 Xujiaping, Lanzhou, Gansu 730046, PR China.
authors.forename: Jifei
authors.initials: J
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, 1 Xujiaping, Lanzhou, Gansu 730046, PR China; Key Laboratory of Veterinary Parasitology of Gansu Province, 1 Xujiaping, Lanzhou, Gansu 730046, PR China; Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, 1 Xujiaping, Lanzhou, Gansu 730046, PR China.
authors.forename: Xinquan
authors.initials: X
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, 1 Xujiaping, Lanzhou, Gansu 730046, PR China; Key Laboratory of Veterinary Parasitology of Gansu Province, 1 Xujiaping, Lanzhou, Gansu 730046, PR China; Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, 1 Xujiaping, Lanzhou, Gansu 730046, PR China.
authors.forename: Guiquan
authors.initials: G
authors.lastname: Guan
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, 1 Xujiaping, Lanzhou, Gansu 730046, PR China; Key Laboratory of Veterinary Parasitology of Gansu Province, 1 Xujiaping, Lanzhou, Gansu 730046, PR China; Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, 1 Xujiaping, Lanzhou, Gansu 730046, PR China.
authors.forename: Aihong
authors.initials: A
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, 1 Xujiaping, Lanzhou, Gansu 730046, PR China; Key Laboratory of Veterinary Parasitology of Gansu Province, 1 Xujiaping, Lanzhou, Gansu 730046, PR China; Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, 1 Xujiaping, Lanzhou, Gansu 730046, PR China.
authors.forename: Jianxun
authors.initials: J
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, 1 Xujiaping, Lanzhou, Gansu 730046, PR China; Key Laboratory of Veterinary Parasitology of Gansu Province, 1 Xujiaping, Lanzhou, Gansu 730046, PR China; Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, 1 Xujiaping, Lanzhou, Gansu 730046, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yin
mesh_terms: D000818:Animals; D019295:Computational Biology; D015870:Gene Expression; D014644:Genetic Variation; D018512:Parasitemia / Q000469:parasitology / Q000662:veterinary; D040901:Proteomics; D015800:Protozoan Proteins / Q000378:metabolism* / Q000502:physiology; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology; D015398:Signal Transduction; D013045:Species Specificity; D013047:Specific Pathogen-Free Organisms; D016794:Theileria / Q000737:chemistry / Q000378:metabolism* / Q000472:pathogenicity / Q000502:physiology; D013801:Theileriasis / Q000469:parasitology; D014357:Trypsin / Q000378:metabolism; D014774:Virulence
chemical_list: D015800:Protozoan Proteins; D014357:Trypsin
keywords: Comparative proteomics; Drug targets; Energy metabolism; Enzyme; Theileria