title: Genetic diversity and relationships detected by ISSR and RAPD analysis among Aethionema species growing in Eastern Anatolia (Turkey).
abstract: In this study, Random amplified polymorphic DNA (RAPD) and Inter-Simple Sequence Repeat (ISSR) analysis were used to examine the genetic relationships among eight Aethionema species (Aethionema caespitosum, A. arabicum, A. cordatum, A. fimnraitum, A. armenum, A. speciosum supsp. speciosum, A. memraneceum, A. grandiflorum var. grandiflorum) growing in the wild in Eastern Anatolia, Turkey. Fourteen RAPD primers and 7 ISSR primers were used. The UPGMA cluster was constructed using a combination of data from RAPD and ISSR markers. The Aethionema species were classified into two major groups. The similarity matrix values of between 0.182 (A. cordatum, A. speciosum supsp. speciosum) and 0.927 (A. grandiflorum var. grandiflorum, A. cordatum). High genetic variations among Aethionema species growing in the wild in Eastern Anatolia, Turkey may reveal differences in their origin. The present study suggests that both RAPD and ISSR analysis are useful for the differentiation of the Aethionema species.
authors.affiliation: Department of Pharmaceutical Botany, Faculty of Pharmacy, Erzincan University, 24000 Erzincan, Turkey. Electronic address: ssunar@erzincan.edu.tr.
authors.forename: Serap
authors.initials: S
authors.lastname: Sunar
authors.affiliation: Department of Biology, Faculty of Science, Erzincan University, 24000 Erzincan, Turkey.
authors.forename: Nalan
authors.initials: N
authors.lastname: Yildirim
authors.affiliation: Department of Biology, Faculty of Science, Atatürk University, 25240 Erzurum, Turkey.
authors.forename: Meryem
authors.initials: M
authors.lastname: Sengul
authors.affiliation: Department of Biology, Faculty of Science, Atatürk University, 25240 Erzurum, Turkey.
authors.forename: Guleray
authors.initials: G
authors.lastname: Agar
mesh_terms: D019607:Brassicaceae / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D014644:Genetic Variation; D018895:Microsatellite Repeats / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D013045:Species Specificity; D014421:Turkey
chemical_list: D017931:DNA Primers
keywords: Aethionema species; Genetic diversity; ISSR; RAPD