title: Effect of oligosaccharides on the adhesion of gut bacteria to human HT-29 cells.
abstract: The influence of five oligosaccharides (cellobiose, stachyose, raffinose, lactulose and chito-oligosaccharides) on the adhesion of eight gut bacteria (Bifidobacterium bifidum ATCC 29521, Bacteroides thetaiotaomicron ATCC 29148D-5, Clostridium leptum ATCC 29065, Blautia coccoides ATCC 29236, Faecalibacterium prausnitzii ATCC 27766, Bacteroides fragilis ATCC 23745, Clostridium difficile ATCC 43255 and Lactobacillus casei ATCC 393) to mucous secreting and non-mucous secreting HT-29 human epithelial cells, was investigated. In pure culture, the bacteria showed variations in their ability to adhere to epithelial cells. The effect of oligosaccharides diminished adhesion and the presence of mucus played a major factor in adhesion, likely due to high adhesiveness to mucins present in the native human mucus layer covering the whole cell surface. However, clostridia displayed almost the same level of adhesion either with or without mucus being present. Bl. coccoides adhesion was decreased by stachyose and cellobiose in non-mucus-secreting cells in pure culture, while in mixed faecal culture cellobiose displayed the highest antiadhesive activity with an overall average of 65% inhibition amongst tested oligomers and lactulose displayed the lowest with an average of 47.4%. Bifidobacteria, Bacteroides, lactobacilli and clostridia were inhibited within the following ranges 47-78%, 32-65%, 11.7-58% and 64-85% respectively. This means that clostridia were the most strongly influenced members of the microflora amongst the bacterial groups tested in mixed culture. In conclusion, introducing oligosaccharides which are candidate prebiotics into pure or mixed cultures has affected bacterial adhesion.
authors.affiliation: Department of Nutrition and Food Technology, An-Najah National University, Nablus, Palestine. Electronic address: m.altamimi@najah.edu.
authors.forename: M
authors.initials: M
authors.lastname: Altamimi
authors.affiliation: Department of Biostatistics, Research Unit, PSMMC, Saudi Arabia. Electronic address: osamaabdelhay@gmail.com.
authors.forename: O
authors.initials: O
authors.lastname: Abdelhay
authors.affiliation: Department of Food and Nutritional Sciences, The University of Reading, UK. Electronic address: r.a.rastall@reading.ac.uk.
authors.forename: R A
authors.initials: RA
authors.lastname: Rastall
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects*; D001439:Bacteroides / Q000187:drug effects / Q000502:physiology; D001441:Bacteroides fragilis / Q000187:drug effects / Q000502:physiology; D001644:Bifidobacterium / Q000187:drug effects / Q000502:physiology; D003013:Clostridium / Q000187:drug effects / Q000502:physiology; D000070036:Faecalibacterium / Q000187:drug effects / Q000502:physiology; D000069196:Gastrointestinal Microbiome / Q000187:drug effects*; D019073:HT29 Cells; D006801:Humans; D007780:Lacticaseibacillus casei / Q000187:drug effects / Q000502:physiology; D009077:Mucins / Q000378:metabolism; D009844:Oligosaccharides / Q000494:pharmacology*; D056692:Prebiotics / Q000032:analysis*; D013045:Species Specificity
chemical_list: D009077:Mucins; D009844:Oligosaccharides; D056692:Prebiotics
keywords: Antiadhesion; Gut microflora; HT-29 cells; Oligosaccharides; Prebiotics