title: Drug-specific in vitro secretion of IFNγ in the diagnosis of drug-induced exanthemas: electrochemiluminescence assay versus previously used diagnostic methods.
abstract: The in vitro diagnosis of delayed drug hypersensitivity reactions remains a research problem. We measured drug-specific IFNγ release in peripheral blood mononuclear cells sampled from patients with drug-induced maculopapular exanthema and the age- and sex-matched control group. This is the first study to directly cross-compare an ultrasensitive assay based on an emerging electrochemiluminescence technology (ECL), the standard lymphocyte proliferation assay and three following tests detecting IFNγ at different steps of its production: intracellular in CD3+CD4+ cells (flow cytometry), secretion at the single cell level (enzyme-linked immunospot assay), bulk content in cell culture supernatant (enzyme-linked immunosorbent assay, ELISA). The highest rate of drug-positive responses were recorded for ELISA and ECL tests (56.25%). No false-positive responses were observed--all tests were negative in the control group. We demonstrated that IFNγ-detecting ELISA is not less efficient than ECL test, however, it is easily available and cheap, which makes it a potential method of choice in the future.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Porebski
authors.forename: Ewa
authors.initials: E
authors.lastname: Czarnobilska
mesh_terms: D000328:Adult; D003875:Drug Eruptions / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D005076:Exanthema / Q000175:diagnosis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007371:Interferon-gamma / Q000097:blood / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D049449:Luminescence; D008297:Male; D008875:Middle Aged
chemical_list: D007371:Interferon-gamma