title: Newly identified allatostatin Bs and their receptor in the two-spotted cricket, Gryllus bimaculatus.
abstract: A cDNA encoding allatostatin Bs (ASTBs) containing the W(X)6W motif was identified using a database generated by a next generation sequencer (NGS) in the two-spotted cricket, Gryllus bimaculatus. The contig sequence revealed the presence of five novel putative ASTBs (GbASTBs) in addition to GbASTBs previously identified in G. bimaculatus. MALDI-TOF MS analyses revealed the presence of these novel and previously identified GbASTBs with three missing GbASTBs. We also identified a cDNA encoding G. bimaculatus GbASTB receptor (GbASTBR) in the NGS data. Phylogenetic analysis demonstrated that this receptor was highly conserved with other insect ASTBRs, including the sex peptide receptor of Drosophila melanogaster. Calcium imaging analyses indicated that the GbASTBR heterologously expressed in HEK293 cells exhibited responses to all identified GbASTBs at a concentration range of 10(-10)-10(-5)M.
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8567, Japan; Research Fellow of Japan Society for the Promotion of Science (JSPS), Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Tsukamoto
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8567, Japan. Electronic address: shinjin@edu.k.u-tokyo.ac.jp.
authors.forename: Shinji
authors.initials: S
authors.lastname: Nagata
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005260:Female; D015870:Gene Expression; D006135:Gryllidae / Q000737:chemistry / Q000235:genetics*; D057809:HEK293 Cells / Q000378:metabolism; D006801:Humans; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D009479:Neuropeptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018013:Receptors, Neuropeptide / Q000235:genetics / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D018076:DNA, Complementary; D019476:Insect Proteins; D009479:Neuropeptides; D018013:Receptors, Neuropeptide; C076523:allatostatin
keywords: Allatostatin-B; Ca(2+) imaging; Gryllus bimaculatus; Receptor