title: Prognostic significance of S-phase fractions in peritumoral invading zone analyzed by laser scanning cytometry in patients with high-grade glioma: A preliminary study.
abstract: The predominant characteristic of malignant glioma is the presence of invading tumor cells in the peritumoral zone. Distinguishing between tumor cells and normal cells in a peritumoral lesion is challenging. Therefore, the aim of the present study was to investigate the cell-cycle phase measurements of fixed paraffin-embedded specimens from the peritumoral invading zone of high-grade gliomas using laser scanning cytometry. A total of 12 high-grade gliomas (2 anaplastic astrocytomas and 10 glioblastomas) were studied. The tumor core and peritumoral invading zone of each tumor specimen were investigated. Tissue sections (50 µm) from the paraffin blocks were deparaffinized, rehydrated and enzymatically disintegrated, and the cells in suspension were stained with propidium iodide and placed on microscope slides. A slight trend for an increased S-phase fraction in the peritumoral invading zone compared with the tumor core was observed (P=0.24). Additionally, there was a trend for a decrease in the overall survival time of patients with increasing peritumoral invading zone S-phase fraction (P=0.12). These data suggest that laser scanning cytometry is a powerful and clinically relevant tool for the objective analysis of the cell cycle in malignant gliomas.
authors.affiliation: Department of Neurosurgery, Brain Research Institute, Niigata University, Niigata 951-8585, Japan; Department of Neurosurgery, Niigata Neurosurgical Hospital, Yamada, Niigata 950-1101, Japan.
authors.forename: Syoichi
authors.initials: S
authors.lastname: Nakajima
authors.affiliation: Department of Neurosurgery, Brain Research Institute, Niigata University, Niigata 951-8585, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Morii
authors.affiliation: Department of Pathology, Brain Research Institute, Niigata University, Niigata 951-8585, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: Department of Neurosurgery, Brain Research Institute, Niigata University, Niigata 951-8585, Japan.
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Fujii
authors.affiliation: Laboratory of Molecular Target Therapy for Cancer, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
authors.forename: Ryuya
authors.initials: R
authors.lastname: Yamanaka
keywords: S phase; cell cycle; high grade glioma; invading zone; laser scanning cytometry; prognostic significance