title: Effects of Medicinal Herb Extracts on In vitro Ruminal Methanogenesis, Microbe Diversity and Fermentation System.
abstract: This study was aimed to evaluate the in vitro effects of medicinal herb extracts (MHEs) on ruminal fermentation characteristics and the inhibition of protozoa to reduce methane production in the rumen. A fistulated Hanwoo was used as a donor of rumen fluid. The MHEs (T1, Veratrum patulum; T2, Iris ensata var. spontanea; T3, Arisaema ringens; T4, Carduus crispus; T5, Pueraria thunbergiana) were added to the in vitro fermentation bottles containing the rumen fluid and medium. Total volatile fatty acid (tVFA), total gas production, gas profiles, and the ruminal microbe communities were measured. The tVFA concentration was increased or decreased as compared to the control, and there was a significant (p<0.05) difference after 24 h incubation. pH and ruminal disappearance of dry matter did not show significant difference. As the in vitro ruminal fermentation progressed, total gas production in added MHEs was increased, while the methane production was decreased compared to the control. In particular, Arisaema ringens extract led to decrease methane production by more than 43%. In addition, the result of real-time polymerase chain reaction indicted that the protozoa population in all added MHEs decreased more than that of the control. In conclusion, the results of this study indicated that MHEs could have properties that decrease ruminal methanogenesis by inhibiting protozoa species and might be promising feed additives for ruminants.
authors.affiliation: National Institute of Animal Science, RDA, Cheonan 31000, Korea.
authors.forename: Eun Tae
authors.initials: ET
authors.lastname: Kim
authors.affiliation: Division of Applied Life Science (BK21 , IALS), Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Hee Soon
authors.initials: HS
authors.lastname: Hwang
authors.affiliation: Easy Bio, Inc., Seoul 06253, Korea.
authors.forename: Sang Min
authors.initials: SM
authors.lastname: Lee
authors.affiliation: Division of Applied Life Science (BK21 , IALS), Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Shin Ja
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Division of Applied Life Science (BK21 , IALS), Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Il Dong
authors.initials: ID
authors.lastname: Lee
authors.affiliation: Division of Applied Life Science (BK21 , IALS), Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Su Kyoung
authors.initials: SK
authors.lastname: Lee
authors.affiliation: Division of Applied Life Science (BK21 , IALS), Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Da Som
authors.initials: da S
authors.lastname: Oh
authors.affiliation: Division of Applied Life Science (BK21 , IALS), Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Jung Hwa
authors.initials: JH
authors.lastname: Lim
authors.affiliation: National Institute of Animal Science, RDA, Cheonan 31000, Korea.
authors.forename: Ho Baek
authors.initials: HB
authors.lastname: Yoon
authors.affiliation: National Institute of Animal Science, RDA, Cheonan 31000, Korea.
authors.forename: Ha Yeon
authors.initials: HY
authors.lastname: Jeong
authors.affiliation: National Institute of Animal Science, RDA, Cheonan 31000, Korea.
authors.forename: Seok Ki
authors.initials: SK
authors.lastname: Im
authors.affiliation: Division of Applied Life Science (BK21 , IALS), Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Sung Sill
authors.initials: SS
authors.lastname: Lee
keywords: Medicinal Herb Extracts; Methane Production; Protozoa; Rumen Fermentation