title: Evaluation of a Whole Blood Chemiluminescent Immunoassay of Interferon-gamma Inducible Protein 10 (IP-10) for Diagnosis of Tuberculosis Patients.
abstract: BACKGROUND
  The rapid diagnosis of bacterium-negative pulmonary TB and extra pulmonary TB is a significant problem in clinical practice.
  
  
  METHODS
  466 individuals were prospectively enrolled, 247 patients with TB, 147 with non-tuberculous pulmonary disease, and 65 healthy controls. Whole blood was stimulated in vitro with rCFP10/ESAT6 antigen of Mycobacterium tuberculosis. A chemiluminescence immunoassay was used to detect the stimulated release of interferon-gamma inducible protein 10 (IP-10), and a receiver operating characteristic (ROC) curve was drawn to determine cutoff value for diagnosing TB and to evaluate the diagnostic efficacy of the IP-10 assay.
  
  
  RESULTS
  The antigen-specific release of IP-10 in the TB group was significantly higher than in the non-tuberculous pulmonary disease group (p < 0.001) and the healthy control group (p < 0.001). The IP-10 test correctly detected more cases of pulmonary TB and extra-pulmonary TB than the acid-fast staining microscopy examination (AFB). The sensitivity, specificity, and diagnostic efficiency for IP-10 were 75.3%, 84.9%, and 79.7%, respectively. Patients with TB and cancer comorbidity produced significantly higher levels of the TB-specific IP-10 release compared to the other groups (p < 0.05), whereas respiratory disease patients produced lower levels than the healthy controls (p > 0.05).
  
  
  CONCLUSIONS
  Our findings indicate high sensitivity and specificity of IP-10 as a novel biomarker for TB in China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Yourong
authors.initials: Y
authors.lastname: Yang
authors.forename: Hang
authors.initials: H
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Liang
authors.forename: Jianyang
authors.initials: J
authors.lastname: Liu
authors.forename: Ting
authors.initials: T
authors.lastname: Yu
authors.forename: Xueqiong
authors.initials: X
authors.lastname: Wu
mesh_terms: D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial / Q000276:immunology; D019540:Area Under Curve; D001426:Bacterial Proteins / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D054357:Chemokine CXCL10 / Q000097:blood*; D002681:China; D015897:Comorbidity; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011237:Predictive Value of Tests; D011446:Prospective Studies; D012372:ROC Curve; D015203:Reproducibility of Results; D014376:Tuberculosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D055815:Young Adult
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D015415:Biomarkers; C117940:CFP-10 protein, Mycobacterium tuberculosis; C114753:CXCL10 protein, human; D054357:Chemokine CXCL10; C093276:ESAT-6 protein, Mycobacterium tuberculosis