title: Resolvin D1 mitigates energy metabolism disorder after ischemia-reperfusion of the rat lung.
abstract: BACKGROUND
  Energy metabolism disorder is a critical process in lung ischemia-reperfusion injury (LIRI). This study was aimed to determine the effects of resolvin D1 (RvD1) on the energy metabolism in LIRI.
  
  
  METHODS
  Forty Sprague-Dawley rats were divided into the following groups: Sham group; untreated ischemia-reperfusion (IR) control; IR treated with normal saline (IR-NS); and IR treated with RvD1 (IR-RV) (100 μg/kg, iv). LIRI and energy metabolism disorder were determined in these rats.
  
  
  RESULTS
  The results revealed that the levels of interleukin (IL)-1β, tumor necrosis factor-α, IL-10, monocyte chemoattractant protein-1, macrophage inflammatory protein-2, cytokine-induced neutrophil chemoattractant-1, injured alveoli rate, apoptosis index, pulmonary permeability index, malondialdehyde, ADP, and lactic acid were increased, whereas the levels of ATP, ATP/ADP, glycogen, Na(+)-K(+)-ATPase, superoxide dismutase, glutathione peroxidase activity, pulmonary surfactant associated protein-A, and oxygenation index were decreased in rats with LIRI. Except for IL-10, all these biomarkers of LIRI and its related energy metabolism disorder were significantly inhibited by RvD1 treatment. In addition, histological analysis via hematoxylin-eosin staining, and transmission electron microscopy confirmed that IR-induced structure damages of lung tissues were reduced by RvD1.
  
  
  CONCLUSIONS
  RvD1 improves the energy metabolism of LIRI disturbance, protects the mitochondrial structure and function, increases the ATP, glycogen content and Na(+)-K(+)-ATPase activity of lung tissue, balances the ratio of ATP/ADP and finally decreases the rate of apoptosis, resulting in the protection of IR-induced lung injury. The improved energy metabolism after LIRI may be related to the reduced inflammatory response, the balance of the oxidative/antioxidant and the pro-inflammatory/anti-inflammatory systems in rats.
authors.affiliation: The Department of Children's Cardiovascular and Thoracic Surgery, Children's Heart Center, the Second Affiliated Hospital, Yuying Children's Hospital, Institute of Cardiovascular Development and Translational Medicine, Wenzhou Medical University, 325000, Wenzhou, People's Republic of China.
authors.forename: Qifeng
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Wuhan Medical & Healthcare Center for Woman and Children, 430015, Wuhan, People's Republic of China.
authors.forename: Ji
authors.initials: J
authors.lastname: Wu
authors.affiliation: The Department of Children's Cardiovascular and Thoracic Surgery, Children's Heart Center, the Second Affiliated Hospital, Yuying Children's Hospital, Institute of Cardiovascular Development and Translational Medicine, Wenzhou Medical University, 325000, Wenzhou, People's Republic of China.
authors.forename: Qingwang
authors.initials: Q
authors.lastname: Hua
authors.affiliation: The Department of Children's Cardiovascular and Thoracic Surgery, Children's Heart Center, the Second Affiliated Hospital, Yuying Children's Hospital, Institute of Cardiovascular Development and Translational Medicine, Wenzhou Medical University, 325000, Wenzhou, People's Republic of China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Lin
authors.affiliation: The Department of Children's Respiration Medicine, The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, 325000, Wenzhou, People's Republic of China.
authors.forename: Leping
authors.initials: L
authors.lastname: Ye
authors.affiliation: The Department of Children's Respiration Medicine, The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, 325000, Wenzhou, People's Republic of China.
authors.forename: Weixi
authors.initials: W
authors.lastname: Zhang
authors.affiliation: The Department of Children's Cardiovascular and Thoracic Surgery, Children's Heart Center, the Second Affiliated Hospital, Yuying Children's Hospital, Institute of Cardiovascular Development and Translational Medicine, Wenzhou Medical University, 325000, Wenzhou, People's Republic of China.
authors.forename: Guowei
authors.initials: G
authors.lastname: Wu
authors.affiliation: The Department of Children's Cardiovascular and Thoracic Surgery, Children's Heart Center, the Second Affiliated Hospital, Yuying Children's Hospital, Institute of Cardiovascular Development and Translational Medicine, Wenzhou Medical University, 325000, Wenzhou, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Du
authors.affiliation: The Department of Children's Cardiovascular and Thoracic Surgery, Children's Heart Center, the Second Affiliated Hospital, Yuying Children's Hospital, Institute of Cardiovascular Development and Translational Medicine, Wenzhou Medical University, 325000, Wenzhou, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xia
authors.affiliation: The Department of Children's Cardiovascular Medicine, Children's Heart Center, the Second Affiliated Hospital, Yuying Children's Hospital, Institute of Cardiovascular Development and Translational Medicine, Wenzhou Medical University, 325000, Wenzhou, People's Republic of China.
authors.forename: Maoping
authors.initials: M
authors.lastname: Chu
authors.affiliation: The Department of Children's Cardiovascular and Thoracic Surgery, Children's Heart Center, the Second Affiliated Hospital, Yuying Children's Hospital, Institute of Cardiovascular Development and Translational Medicine, Wenzhou Medical University, 325000, Wenzhou, People's Republic of China. huxingti@126.com.
authors.forename: Xingti
authors.initials: X
authors.lastname: Hu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D004281:Docosahexaenoic Acids / Q000494:pharmacology / Q000627:therapeutic use*; D004734:Energy Metabolism / Q000187:drug effects*; D007249:Inflammation / Q000150:complications / Q000473:pathology; D008168:Lung / Q000098:blood supply* / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D055370:Lung Injury / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000150:complications / Q000188:drug therapy* / Q000378:metabolism* / Q000503:physiopathology; D012129:Respiratory Function Tests
chemical_list: C518399:resolvin D1; D004281:Docosahexaenoic Acids
keywords: Energy metabolism; Inflammatory factor; Lung ischemia/reperfusion injury; Oxidative stress; Resolvin