title: Molecular Methods for Identification of Monophasic Salmonella Typhimurium Strains.
abstract: Two molecular biology methods were used to differentiate Salmonella enterica 1,4,[5],12:i:- strains: "Salmonella Check&Trace microarray" (CT) and multiplex PCR (mPCR). For 92 strains in CT result "Salmonella 1,4,[5],12:i:-" were obtained. Those strains were confirmed in mPCR as monophasic fljB-lack Salmonella Typhimurium. For 17 strains, which in CT assay were recognized as Salmonella Typhimurium, the same identification was obtained in mPCR. Reference Salmonella strains: Lagos, Agama, Tsevie, Glocester and Tumodi in CT were recognized as Salmonella genovar, in mPCR--as Salmonella O:4, H:i other than Salmonella Typhimurium, the same like Salmonella Farsta, recognized incorrectly in CT as Salmonella Typhimurium.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Madajczak
authors.forename: Bozena
authors.initials: B
authors.lastname: Dera-Tomaszewska
authors.forename: Monika
authors.initials: M
authors.lastname: Wasiak
authors.forename: Anna
authors.initials: A
authors.lastname: Chróst
authors.forename: Jolanta
authors.initials: J
authors.lastname: Szych
mesh_terms: D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D040081:Protein Array Analysis / Q000379:methods*; D012486:Salmonella typhimurium / Q000145:classification / Q000235:genetics*; D065288:Serogroup
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial