title: Is There Excitation Energy Transfer between Different Layers of Stacked Photosystem-II-Containing Thylakoid Membranes?
abstract: We have compared picosecond fluorescence decay kinetics for stacked and unstacked photosystem II membranes in order to evaluate the efficiency of excitation energy transfer between the neighboring layers. The measured kinetics were analyzed in terms of a recently developed fluctuating antenna model that provides information about the dimensionality of the studied system. Independently of the stacking state, all preparations exhibited virtually the same value of the apparent dimensionality, d = 1.6. Thus, we conclude that membrane stacking does not affect the efficiency of the delivery of excitation energy toward the reaction centers but ensures a more compact organization of the thylakoid membranes within the chloroplast and separation of photosystems I and II.
authors.affiliation: Laboratory of Biophysics, Wageningen University , P.O. Box 8128, 6700 ET Wageningen, The Netherlands.
authors.forename: Shazia
authors.initials: S
authors.lastname: Farooq
authors.affiliation: Department of Theoretical Physics, Faculty of Physics, Vilnius University , Saulėtekio Avenue 9, 10222 Vilnius, Lithuania.|Department of Molecular Compound Physics, Institute of Physics, Center for Physical Sciences and Technology , Goštauto 11, 01108 Vilnius, Lithuania.
authors.forename: Jevgenij
authors.initials: J
authors.lastname: Chmeliov
authors.affiliation: Department of Theoretical Physics, Faculty of Physics, Vilnius University , Saulėtekio Avenue 9, 10222 Vilnius, Lithuania.|Department of Molecular Compound Physics, Institute of Physics, Center for Physical Sciences and Technology , Goštauto 11, 01108 Vilnius, Lithuania.
authors.forename: Gediminas
authors.initials: G
authors.lastname: Trinkunas
authors.affiliation: Department of Theoretical Physics, Faculty of Physics, Vilnius University , Saulėtekio Avenue 9, 10222 Vilnius, Lithuania.|Department of Molecular Compound Physics, Institute of Physics, Center for Physical Sciences and Technology , Goštauto 11, 01108 Vilnius, Lithuania.
authors.forename: Leonas
authors.initials: L
authors.lastname: Valkunas
authors.affiliation: Laboratory of Biophysics, Wageningen University , P.O. Box 8128, 6700 ET Wageningen, The Netherlands.|MicroSpectroscopy Centre, Wageningen University , P.O. Box 8128, 6700 ET Wageningen, The Netherlands.
authors.forename: Herbert
authors.initials: H
authors.lastname: van Amerongen
mesh_terms: D004735:Energy Transfer; D008958:Models, Molecular; D045332:Photosystem II Protein Complex / Q000378:metabolism*; D018724:Spinacia oleracea / Q000166:cytology / Q000378:metabolism*; D020524:Thylakoids / Q000378:metabolism*
chemical_list: D045332:Photosystem II Protein Complex