title: [Comparative research into sensitivity and specificity of immune-enzyme analysis with chemiluminescence and colorimetric detection for detecting antigens and antibodies to avian influenza viruses and newcastle disease].
abstract: The goal of this work was to demonstrate the results of the development of the enzyme-linked immunosorbent tests with chemiluminescence detection and colorimetric detection of specific viral antigens and antibodies for identifying the avian influenza and the Newcastle disease viruses: high sensitivity and specificity of the immuno- chemiluminescence assay, which are 10-50 times higher than those of the ELISA colorimetric method. The high effectiveness of the results and the automation of the process of laboratory testing (using a luminometer) allow these methods to be recommended for including in primary screening tests for these infectious diseases.
authors.forename: O N
authors.initials: ON
authors.lastname: Vitkova
authors.forename: T P
authors.initials: TP
authors.lastname: Kapustina
authors.forename: V V
authors.initials: VV
authors.lastname: Mikhailova
authors.forename: G A
authors.initials: GA
authors.lastname: Safonov
authors.forename: N N
authors.initials: NN
authors.lastname: Vlasova
authors.forename: R V
authors.initials: RV
authors.lastname: Belousova
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000097:blood*; D001681:Biological Assay; D002645:Chickens; D003124:Colorimetry; D004797:Enzyme-Linked Immunosorbent Assay; D006385:Hemagglutination Inhibition Tests; D009980:Influenza A virus / Q000276:immunology; D005585:Influenza in Birds / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D049449:Luminescence; D009521:Newcastle Disease / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D009522:Newcastle disease virus / Q000276:immunology; D011201:Poultry Diseases / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral