title: Development of an activity-based probe for amyloid β-hydrolyzing antibodies.
abstract: We report developing an activity-based probe containing an amyloid β peptide (Aβ) 17-27 and an electrophilic phosphonate diester at the C-terminus. A probe containing an electrophilic moiety is able to react with the nucleophiles on an antibody or an antibody with proteinase activity. The probe reacted with an Aβ specific monoclonal antibody and formed a covalent complex. The covalent binding also occurred specifically when the probe reacted with serum containing anti-Aβ antibodies. These results suggest that the probe would serve as a powerful tool to isolate Aβ specific antibodies that are capable of Aβ hydrolysis activity.
authors.affiliation: Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science, 3500-3, Minamitamagaki, Suzuka, Mie 513-8670, Japan. Electronic address: taguchi@suzuka-u.ac.jp.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Taguchi
authors.affiliation: Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science, 3500-3, Minamitamagaki, Suzuka, Mie 513-8670, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Fujita
authors.affiliation: Faculty of Pharmaceutical Sciences, Kobe Gakuin University, Chuo-ku, Kobe 650-8586, Japan; Cooperative Research Center of Life Sciences, Kobe Gakuin University, Chuo-ku, Kobe 650-8586, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Tsuda
mesh_terms: D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000276:immunology*; D000906:Antibodies / Q000276:immunology*; D006868:Hydrolysis
chemical_list: D016229:Amyloid beta-Peptides; D000906:Antibodies
keywords: Activity-based probe; Alzheimer’s disease; Amyloid β peptide; Antibodies; Phosphonate diester