title: High-density lipopoprotein antioxidant capacity, subpopulation distribution and paraoxonase-1 activity in patients with systemic lupus erythematosus.
abstract: BACKGROUND
  The causes of increased cardiovascular risk in systemic lupus erythematosus (SLE) are not understood thoroughly, although presence of traditional cardiovascular risk factors and disease-specific agents were also proposed. In this study, we investigated the quantitative changes in the lipid profile, as well as qualitative characteristics of high-density lipoprotein (HDL) and markers of inflammation and disease activity in SLE patients.
  
  
  METHODS
  Lipoprotein levels were determined in 51 SLE patients and 49 healthy controls, matched in age and gender. HDL antioxidant capacity was determined spectrophotometrically with a cell-free method of hemin-induced low-density lipoprotein (LDL) oxidation. Polyacrylamide gel-electrophoresis was used for HDL subfraction analysis. Human paraoxonase-1 (PON1) activity, apolipoprotein A1 (ApoA1) and oxidized LDL concentrations, as well as interleukin-6, high-sensitivity C-reactive protein, serum amyloid A and monocyte chemotactic protein-1 levels were determined.
  
  
  RESULTS
  HDL-cholesterol and ApoA1 concentrations decreased significantly in SLE subjects. Also, PON1 arylesterase activity (125.65 ± 26.87 vs. 148.35 ± 39.34 U/L, p = 0.001) and total HDL antioxidant capacity (165.82 ± 58.28% vs. 217.71 ± 54.36%, p < 0.001) were significantly reduced in patients compared to controls. Additionally, all HDL subfraction concentrations were significantly decreased in patients, while the levels of the examined inflammatory markers were significantly elevated in SLE subjects. The latter correlated positively with disease activity, and negatively with HDL concentration and total HDL antioxidant capacity, respectively. PON1 arylesterase activity and erythrocyte sedimentation rate were independent predictors of total HDL antioxidant capacity.
  
  
  CONCLUSIONS
  Induced by the systemic inflammation, altered composition and antioxidant activity may diminish the anti-atherogenic effect of HDL and therefore may contribute to the increased cardiovascular risk of SLE patients.
authors.affiliation: Department of Medicine, Faculty of Medicine, University of Debrecen, krt. 98., Debrecen, H-4032, Hungary.
authors.forename: Krisztina
authors.initials: K
authors.lastname: Gaál
authors.affiliation: Department of Medicine, Faculty of Medicine, University of Debrecen, krt. 98., Debrecen, H-4032, Hungary.
authors.forename: Tünde
authors.initials: T
authors.lastname: Tarr
authors.affiliation: Department of Medicine, Faculty of Medicine, University of Debrecen, krt. 98., Debrecen, H-4032, Hungary.
authors.forename: Hajnalka
authors.initials: H
authors.lastname: Lőrincz
authors.affiliation: Department of Medicine, Faculty of Medicine, University of Debrecen, krt. 98., Debrecen, H-4032, Hungary.
authors.forename: Viktor
authors.initials: V
authors.lastname: Borbás
authors.affiliation: Department of Medicine, Faculty of Medicine, University of Debrecen, krt. 98., Debrecen, H-4032, Hungary.
authors.forename: Ildikó
authors.initials: I
authors.lastname: Seres
authors.affiliation: Department of Medicine, Faculty of Medicine, University of Debrecen, krt. 98., Debrecen, H-4032, Hungary.
authors.forename: Mariann
authors.initials: M
authors.lastname: Harangi
authors.affiliation: Department of Medicine, Faculty of Medicine, University of Debrecen, krt. 98., Debrecen, H-4032, Hungary.
authors.forename: Péter
authors.initials: P
authors.lastname: Fülöp
authors.affiliation: Department of Medicine, Faculty of Medicine, University of Debrecen, krt. 98., Debrecen, H-4032, Hungary. paragh@belklinika.com.
authors.forename: György
authors.initials: G
authors.lastname: Paragh
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D016632:Apolipoprotein A-I / Q000097:blood; D043303:Aryldialkylphosphatase / Q000097:blood* / Q000378:metabolism; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D016022:Case-Control Studies; D008076:Cholesterol, HDL / Q000097:blood; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000378:metabolism; D015850:Interleukin-6 / Q000097:blood; D008075:Lipoproteins, HDL / Q000097:blood* / Q000378:metabolism; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000378:metabolism*; D008297:Male; D018384:Oxidative Stress
chemical_list: C505693:APOA1 protein, human; D000975:Antioxidants; D016632:Apolipoprotein A-I; D015415:Biomarkers; D008076:Cholesterol, HDL; C508600:IL6 protein, human; D015850:Interleukin-6; D008075:Lipoproteins, HDL; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human
keywords: HDL antioxidant capacity; HDL subfractions; SLE; cardiovascular disease; oxidized LDL; paraoxonase-1