title: Development of a biosensor for electrochemical detection of tumor-associated proteins in blood plasma of cancer patients by aptamers.
abstract: A molecular biosensor based on DNA aptamers (aptasensor) for the diagnosis of lung cancer in blood plasma samples was designed. To create the aptasensor, the aptamer 17_80, obtained in the study of postoperative material, was used. The affinity and binding selectivity of the aptamer 17_80 to the lung tumor tissue was confirmed on histological sections of postmortem samples of lung tissue. Using affinity enrichment and mass spectrometry, a possible target molecule of the aptamer 17_80, vimentin, was found.
authors.affiliation: Institute of Chemistry and Chemical Technology, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russia.|Voino-Yasenetskii State Medical University, Ministry of Health of the Russian Federation, ul. Partizana Zheleznyaka 1, Krasnoyarsk, Krasnoyarsk Krai, 660022, Russia.
authors.forename: G S
authors.initials: GS
authors.lastname: Zamay
authors.affiliation: Voino-Yasenetskii State Medical University, Ministry of Health of the Russian Federation, ul. Partizana Zheleznyaka 1, Krasnoyarsk, Krasnoyarsk Krai, 660022, Russia. tzamay@yhandex.ru.
authors.forename: T N
authors.initials: TN
authors.lastname: Zamay
authors.affiliation: Institute of Chemistry and Chemical Technology, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russia.|Voino-Yasenetskii State Medical University, Ministry of Health of the Russian Federation, ul. Partizana Zheleznyaka 1, Krasnoyarsk, Krasnoyarsk Krai, 660022, Russia.
authors.forename: O S
authors.initials: OS
authors.lastname: Kolovskaya
authors.affiliation: Kryzhanovsky Krasnoyarsk Regional Cancer Clinic, Krasnoyarsk, Krasnoyarsk Krai, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Krat
authors.affiliation: Institute of Chemistry and Chemical Technology, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russia.|Voino-Yasenetskii State Medical University, Ministry of Health of the Russian Federation, ul. Partizana Zheleznyaka 1, Krasnoyarsk, Krasnoyarsk Krai, 660022, Russia.
authors.forename: Yu E
authors.initials: YE
authors.lastname: Glazyrin
authors.affiliation: Institute of Chemistry and Chemical Technology, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russia.|Voino-Yasenetskii State Medical University, Ministry of Health of the Russian Federation, ul. Partizana Zheleznyaka 1, Krasnoyarsk, Krasnoyarsk Krai, 660022, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Dubinina
authors.affiliation: Institute of Chemistry and Chemical Technology, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russia.|Voino-Yasenetskii State Medical University, Ministry of Health of the Russian Federation, ul. Partizana Zheleznyaka 1, Krasnoyarsk, Krasnoyarsk Krai, 660022, Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Zamay
mesh_terms: D000328:Adult; D000368:Aged; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000494:pharmacology; D014408:Biomarkers, Tumor / Q000097:blood* / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D002294:Carcinoma, Squamous Cell / Q000097:blood*; D055088:Early Detection of Cancer / Q000379:methods*; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000097:blood* / Q000378:metabolism; D015513:Oncogene Proteins; D011485:Protein Binding
chemical_list: D052157:Aptamers, Nucleotide; D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D015513:Oncogene Proteins