title: Further evidence from SSCP and ITS DNA sequencing support Trypanosoma evansi and Trypanosoma equiperdum as subspecies or even strains of Trypanosoma brucei.
abstract: The subgenus Trypanozoon includes three species Trypanosoma brucei, Trypanosoma evansi and Trypanosoma equiperdum, which are morphologically identical and indistinguishable even using some molecular methods. In this study, PCR-based single strand conformation polymorphism (PCR-SSCP) was used to analyze the ribosomal DNA of the Trypanozoon species. Data indicate different patterns of ITS2 fragments between T. brucei, T. evansi and T. equiperdum by SSCP. Furthermore, analysis of total ITS sequences within these three members of the subgenus Trypanozoon showed a high degree of homology using phylogenetic analysis but were polyphyletic in haplotype networks. These data provide novel nuclear evidence to further support the notion that T. evansi and T. equiperdum should be subspecies or even strains of T. brucei.
authors.affiliation: Center for Parasitic Organisms, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
authors.forename: Yan-Zi
authors.initials: YZ
authors.lastname: Wen
authors.affiliation: Center for Parasitic Organisms, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China; State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China; Ecosystems & Environment Centre, School of Environment and Life Sciences, University of Salford, Salford M5 4WT, UK; Biomedical Research Centre, School of Environment and Life Sciences, University of Salford, Salford M5 4WT, UK.
authors.forename: Zhao-Rong
authors.initials: ZR
authors.lastname: Lun
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China.
authors.forename: Xing-Quan
authors.initials: XQ
authors.lastname: Zhu
authors.affiliation: Ecosystems & Environment Centre, School of Environment and Life Sciences, University of Salford, Salford M5 4WT, UK; Biomedical Research Centre, School of Environment and Life Sciences, University of Salford, Salford M5 4WT, UK.
authors.forename: Geoff
authors.initials: G
authors.lastname: Hide
authors.affiliation: Center for Parasitic Organisms, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China. Electronic address: laidehua@mail.sysu.edu.cn.
authors.forename: De-Hua
authors.initials: DH
authors.lastname: Lai
mesh_terms: D000349:Africa / Q000453:epidemiology; D000818:Animals; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D016054:DNA, Protozoan / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D007303:Insect Vectors / Q000469:parasitology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014345:Trypanosoma / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014346:Trypanosoma brucei brucei / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014352:Trypanosomiasis / Q000453:epidemiology* / Q000469:parasitology / Q000635:transmission; D014370:Tsetse Flies / Q000469:parasitology
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal
keywords: Haplotype network; ITS; PCR-SSCP; Phylogenetic tree; Trypanozoon