title: In vitro evaluation of silver nanoparticles cytotoxicity on Hepatic cancer (Hep-G2) cell line and their antioxidant activity: Green approach for fabrication and application.
abstract: In this article, biosynthesis of silver nanoparticles (AgNPs) using Andean Mora (Rubus glaucus Benth.) leaf has been reported. Different analytical techniques including UV-vis spectroscopy, dynamic light scattering (DLS), transmission electron microscopy (TEM) and X-ray diffraction (XRD) were used for the characterization of AgNPs. The initial appearance of color change with the intense surface plasmon resonance (SPR) bands around 440-455 in UV-visible spectra revealing the formation of AgNPs. The TEM image showed the AgNPs to be anisotropic, quasi-spherical in shape with sizes in the range of 12-50nm. On the other hand, XRD studies revealed the formation of face-centered cubic structure for AgNPs. The surface modified AgNPs showed no cytotoxicity at the concentration ranging from 0.01μM to 1.0μM on the Hepatic cancer (Hep-G2) cell line and observed antioxidant efficacy >70% at the concentration 0.05mM/0.20mL against 1, 1-diphenyl-2-picrylhydrazyl. From the results obtained it is suggested that AgNPs could be used effectively in future drug delivery systems and other biomedical concerns.
authors.affiliation: Centro de Nanociencia y Nanotecnologia, Universidad de las Fuerzas Armadas ESPE, Av. Gral. Rumiñahui s/n, Sangolqui, P.O. BOX 171-5-231B, Ecuador. Electronic address: krmbraj@gmail.com.
authors.forename: Brajesh
authors.initials: B
authors.lastname: Kumar
authors.affiliation: Centro de Nanociencia y Nanotecnologia, Universidad de las Fuerzas Armadas ESPE, Av. Gral. Rumiñahui s/n, Sangolqui, P.O. BOX 171-5-231B, Ecuador.
authors.forename: Kumari
authors.initials: K
authors.lastname: Smita
authors.affiliation: Centro de Nanociencia y Nanotecnologia, Universidad de las Fuerzas Armadas ESPE, Av. Gral. Rumiñahui s/n, Sangolqui, P.O. BOX 171-5-231B, Ecuador.
authors.forename: Rachid
authors.initials: R
authors.lastname: Seqqat
authors.affiliation: Centro de Nanociencia y Nanotecnologia, Universidad de las Fuerzas Armadas ESPE, Av. Gral. Rumiñahui s/n, Sangolqui, P.O. BOX 171-5-231B, Ecuador.
authors.forename: Karen
authors.initials: K
authors.lastname: Benalcazar
authors.affiliation: Centro de Nanociencia y Nanotecnologia, Universidad de las Fuerzas Armadas ESPE, Av. Gral. Rumiñahui s/n, Sangolqui, P.O. BOX 171-5-231B, Ecuador.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Grijalva
authors.affiliation: Centro de Nanociencia y Nanotecnologia, Universidad de las Fuerzas Armadas ESPE, Av. Gral. Rumiñahui s/n, Sangolqui, P.O. BOX 171-5-231B, Ecuador.
authors.forename: Luis
authors.initials: L
authors.lastname: Cumbal
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D049109:Cell Proliferation; D004354:Drug Screening Assays, Antitumor; D056945:Hep G2 Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D012834:Silver / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods; D014961:X-Ray Diffraction
chemical_list: D000975:Antioxidants; D012834:Silver
keywords: Antioxidant; FTIR; Green synthesis; Hep-G2 cells; Silver nanoparticles; TEM