title: [COMPOSITION OF GASTRIC JUICE AND BILE IN RATS AT THE EXPERIMENTAL CHRONIC PANCREATITIS].
abstract: Chronic pancreatitis is an inflammatory disease of the pancreas, which is characterized by destruction of pancreatic secretory parenchyma and progressing exocrine and endocrine insufficiency. Usually these patients have complications as cardiovascular, renal, respiratory and liver failure, and various gastric dysfunctions. The data of clinical observations do not reveal fully the functional state of the stomach and liver in chronic pancreatitis also remains an open question about the quality of the gastric juices and bile by this pathology. Therefore our aim was to investigate the secretory functions of the stomach and liver features in rats at the experimental chronic pancreatitis. This pathology modeled using L-arginine. Basal gastric secretion was investigated in chronic experiment by aspiration method for 10th and 63rd days, and pancreas and liver--in acute experiments at 13th and 68th days after the last administration of L-arginine. It was established that the character of the secretory response of the digestive tract depends on the duration of the pathology course. On the 10th day the functional state of the gastric secretory glands in rats with chronic pancreatitis characterized by twice increase of gastric acid production but decrease the level of hexosamines on 23.8% (P < 0.001) that indicate a increase of gastric content aggressiveness and mucus producing cells secretory insufficiency. In these animals the rate of total protein decreased on 61.7% (P < 0.05). On the 13th day observed the increase of pancreatic juice on 332% (P < 0.01), hepatic secret volume on 74.9% (P < 0.001) and redistribution in the cholates spectrum: glycocholates level increased but tauro-, free and total dehydroxylated bile acids decreased. These changes suggest deterioration of bile detergent properties, inhibition of acidic pathway of bile acids biosynthesis and conjugation of cholates with taurine. In two months total deficit of amino acids in gastric juice correlated with exocrine pancreatic insufficiency. Herein the acidity of gastric content partially restored, while the level of protein and mucus secretion proceed to decline. Consequently gastric mucosa is more vulnerable. In these rats the rates of free bile acids greatly increased while tauro- and glycocholates significantly decreased. Thus the processes of hydroxylation and conjugation of bile acids with amino acids inhibited suggesting interruption of synthetic and detoxification functions of the liver. The present work is important for comprehension the pathophysiological aspects of chronic pancreatitis particularly the digestive system functioning features at this pathology. These data could be considered in the appointment of treatment to avoid complications.
authors.forename: Z A
authors.initials: ZA
authors.lastname: Gorenko
authors.forename: O A
authors.initials: OA
authors.lastname: Grinchenko
authors.forename: S P
authors.initials: SP
authors.lastname: Veselsky
authors.forename: V M
authors.initials: VM
authors.lastname: Baban
mesh_terms: D000818:Animals; D021461:Animals, Outbred Strains; D001120:Arginine; D001646:Bile / Q000737:chemistry*; D001647:Bile Acids and Salts / Q000096:biosynthesis / Q000737:chemistry; D020355:Cholates / Q000378:metabolism; D005750:Gastric Juice / Q000737:chemistry*; D005753:Gastric Mucosa / Q000378:metabolism* / Q000473:pathology; D006595:Hexosamines / Q000096:biosynthesis; D006900:Hydroxylation; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D010179:Pancreas / Q000378:metabolism / Q000473:pathology; D050500:Pancreatitis, Chronic / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism; D051381:Rats; D013270:Stomach / Q000473:pathology; D013654:Taurine / Q000378:metabolism
chemical_list: D001647:Bile Acids and Salts; D020355:Cholates; D006595:Hexosamines; D011506:Proteins; D013654:Taurine; D001120:Arginine