title: The retinoid X receptor from mud crab: new insights into its roles in ovarian development and related signaling pathway.
abstract: In arthropods, retinoid X receptor (RXR) is a highly conserved nuclear hormone receptor. By forming a heterodimeric complex with the ecdysone receptor (EcR), RXR is known to be vital importance for various physiological processes. However, in comparison to EcR, the RXR signaling pathway and its roles in crustacean reproduction are poorly understood. In the present study, the RXR mRNA was detected in the ovarian follicular cells of mud crab Scylla paramamosain (SpRXR) and during ovarian maturation, its expression level was found to increase significantly. In vitro experiment showed that both SpRXR and vitellogenin (SpVg) mRNA in the ovarian explants were significantly induced by 20-hydroxyecdysone (20E) but not methyl farnesoate (MF). However, differing from the in vitro experiment, injection of MF in in vivo experiment significantly stimulated the expressions of SpRXR and SpVg in female crabs at early vitellogenic stage, but the ecdysone and insect juvenile hormone (JH) signaling pathway genes were not induced. The results together suggest that both MF and SpRXR play significant roles in regulating the expression of SpVg and ovarian development of S. paramamosain through their own specific signaling pathway rather than sharing with the ecdysone or the insect JH.
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China.|School of Life Sciences, Nantong University, Nantong 226007, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Gong
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China.
authors.forename: Chencui
authors.initials: C
authors.lastname: Huang
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Shu
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China.
authors.forename: Chenchang
authors.initials: C
authors.lastname: Bao
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China.
authors.forename: Huiyang
authors.initials: H
authors.lastname: Huang
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China.|Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361102, China.
authors.forename: Haihui
authors.initials: H
authors.lastname: Ye
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China.|College of Marine and Environmental Sciences, James Cook University, Townsville, Queensland 4811, Australia.
authors.forename: Chaoshu
authors.initials: C
authors.lastname: Zeng
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China.
authors.forename: Shaojing
authors.initials: S
authors.lastname: Li
mesh_terms: D000818:Animals; D003386:Brachyura / Q000235:genetics* / Q000254:growth & development; D003001:Cloning, Molecular; D004441:Ecdysterone / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D010053:Ovary / Q000254:growth & development* / Q000378:metabolism; D010802:Phylogeny; D011987:Receptors, Steroid / Q000235:genetics; D047488:Retinoid X Receptors / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D014819:Vitellogenins / Q000235:genetics*
chemical_list: D005231:Fatty Acids, Unsaturated; D011987:Receptors, Steroid; D047488:Retinoid X Receptors; D014819:Vitellogenins; C055469:ecdysone receptor; C032429:methyl farnesoate; D004441:Ecdysterone