title: Studies on Liquefaction Time and Proteins Involved in the Improvement of Seminal Characteristics in Dromedary Camels (Camelus dromedarius).
abstract: Semen was collected from six dromedary camels using artificial vagina during rutting season. Liquefaction of the viscous semen occurred in 23.89 ± 1.49 h. During liquefaction, proteins with molecular masses of 24.55 kDa and 22.07 kDa appeared in conjunction with the disappearance of intact 26.00 kDa protein after 18-24 h. These proteins were identified as β-nerve growth factors (β-NGFs) in liquefied camel semen. Guanidine-HCL improves the rheological characteristics of dromedary camel semen along with significant (P < 0.01) increase in sperm motility. No significant differences were found in viability of spermatozoa indicating no visible detrimental effects on spermatozoa. The cause of semen viscosity, as well as proteins that are present in liquefied dromedary camel seminal plasma, is described for the first time.
authors.affiliation: National Research Centre on Camel, Bikaner, Rajasthan 334 001, India; Indian Veterinary Research Institute, Regional Station, Palampur 176 061, India.
authors.forename: Gorakh
authors.initials: G
authors.lastname: Mal
authors.affiliation: National Research Centre on Camel, Bikaner, Rajasthan 334 001, India.
authors.forename: Sumant
authors.initials: S
authors.lastname: Vyas
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110 029, India.
authors.forename: Alagiri
authors.initials: A
authors.lastname: Srinivasan
authors.affiliation: National Research Centre on Camel, Bikaner, Rajasthan 334 001, India.
authors.forename: Nitin Vasant Rao
authors.initials: NV
authors.lastname: Patil
authors.affiliation: Indian Council of Agricultural Research (ICAR), Krishi Bhavan, New Delhi 110 001, India.
authors.forename: Krishan Murari Lal
authors.initials: KM
authors.lastname: Pathak