title: Glial cell derived neurotrophic factor induces spermatogonial stem cell marker genes in chicken mesenchymal stem cells.
abstract: Mesenchymal stem cells (MSCs) are known with the potential of multi-lineage differentiation. Advances in differentiation technology have also resulted in the conversion of MSCs to other kinds of stem cells. MSCs are considered as a suitable source of cells for biotechnology purposes because they are abundant, easily accessible and well characterized cells. Nowadays small molecules are introduced as novel and efficient factors to differentiate stem cells. In this work, we examined the potential of glial cell derived neurotrophic factor (GDNF) for differentiating chicken MSCs toward spermatogonial stem cells. MSCs were isolated and characterized from chicken and cultured under treatment with all-trans retinoic acid (RA) or glial cell derived neurotrophic factor. Expression analysis of specific genes after 7days of RA treatment, as examined by RT-PCR, proved positive for some germ cell markers such as CVH, STRA8, PLZF and some genes involved in spermatogonial stem cell maintenance like BCL6b and c-KIT. On the other hand, GDNF could additionally induce expression of POU5F1, and NANOG as well as other genes which were induced after RA treatment. These data illustrated that GDNF is relatively more effective in diverting chicken MSCs towards Spermatogonial stem cell -like cells in chickens and suggests GDNF as a new agent to obtain transgenic poultry, nevertheless, exploitability of these cells should be verified by more experiments.
authors.affiliation: Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Sohrab
authors.initials: S
authors.lastname: Boozarpour
authors.affiliation: Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran; Cell and Molecular Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran; Research Group of Stem Cells and Regenerative Medicine, Academic Center for Education, Culture and Research, Mashhad Branch, Mashhad, Iran.
authors.forename: Maryam M
authors.initials: MM
authors.lastname: Matin
authors.affiliation: Department of Biology, Faculty of Sciences, Hakim Sabzevari University, Sabzevar, Iran.
authors.forename: Madjid
authors.initials: M
authors.lastname: Momeni-Moghaddam
authors.affiliation: Cell and Molecular Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran; Department of Biotechnology, Faculty of Veterinary Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Hesam
authors.initials: H
authors.lastname: Dehghani
authors.affiliation: Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Naser
authors.initials: N
authors.lastname: Mahdavi-Shahri
authors.affiliation: Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran.
authors.forename: Sajjad
authors.initials: S
authors.lastname: Sisakhtnezhad
authors.affiliation: Research Group of Stem Cells and Regenerative Medicine, Academic Center for Education, Culture and Research, Mashhad Branch, Mashhad, Iran.
authors.forename: Asieh
authors.initials: A
authors.lastname: Heirani-Tabasi
authors.affiliation: Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran; Research Group of Stem Cells and Regenerative Medicine, Academic Center for Education, Culture and Research, Mashhad Branch, Mashhad, Iran.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Irfan-Maqsood
authors.affiliation: Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran; Cell and Molecular Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran; Research Group of Stem Cells and Regenerative Medicine, Academic Center for Education, Culture and Research, Mashhad Branch, Mashhad, Iran. Electronic address: ar-bahrami@um.ac.ir.
authors.forename: Ahmad Reza
authors.initials: AR
authors.lastname: Bahrami
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002645:Chickens; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000008:administration & dosage*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D013093:Spermatogonia / Q000187:drug effects* / Q000254:growth & development; D014157:Transcription Factors / Q000096:biosynthesis; D014212:Tretinoin / Q000008:administration & dosage
chemical_list: C495039:GDNF protein, human; D051100:Glial Cell Line-Derived Neurotrophic Factor; D014157:Transcription Factors; D014212:Tretinoin
keywords: Differentiation; Glial cell derived neurotrophic factor; Mesenchymal stem cell; Retinoic acid