title: Detection and identification of infectious bronchitis virus by RT-PCR in Iran.
abstract: Infectious bronchitis virus (IBV) causes severe diseases in poultry with significant economic consequences to the poultry industry in Iran. The aim of this study was the detection and identification of IBV by reverse transcription(RT)-PCR in Iran. Ten IB virus strains were detected by testing trachea, cecal tonsil, and kidney tissues collected from broiler and layer farms in Iran. In order to detect infectious bronchitis virus, an optimized RT-PCR was used. Primers targeting the conserved region of known IBV serotypes were used in the RT-PCR assay. Primers selectively detecting Massachusetts and 793/B type IB viruses were designed to amplify the S1 gene of the virus and used in the nested PCR test. Our findings indicate the circulation of at least three genotypes of IB viruses (Massachusetts, 793/B, and variant 2) among poultry flocks.
authors.affiliation: Symoorgh Avian Special Clinic, Tehran, Iran. homayounimehr@hotmail.com.
authors.forename: Alireza
authors.initials: A
authors.lastname: Homayounimehr
authors.affiliation: Symoorgh Avian Special Clinic, Tehran, Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Pakbin
authors.affiliation: Avian Disease and Scientific Research Department, Razi Vaccine and Serum Research Institute, Karaj, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Momayyez
authors.affiliation: Symoorgh Avian Special Clinic, Tehran, Iran.
authors.forename: Seyyedeh Mahsa Rastegar
authors.initials: SM
authors.lastname: Fatemi
mesh_terms: D000818:Animals; D002645:Chickens; D018352:Coronavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D001351:Infectious bronchitis virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D007492:Iran / Q000453:epidemiology; D011201:Poultry Diseases / Q000453:epidemiology* / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D014764:Viral Proteins
keywords: 793/B; IBV in Iran; Massachusetts; RT-PCR