title: Ex Vivo Biomechanical Comparison of 4 Suture Materials for Laparoscopic Bladder Closure in the Horse.
abstract: OBJECTIVE
  To compare a knotless, barbed suture to standard suture using laparoscopic suturing methods in an ex vivo model of equine bladder repair.
  
  
  METHODS
  Cadaveric study.
  
  
  METHODS
  Equine cadaver bladders (n=42).
  
  
  METHODS
  A 5-cm incision was created and repaired in a laparoscopic training box with 4 different suture materials. Groups 1 and 2 used 2-0 poliglecaprone and 2-0 glycomer knotless, barbed suture, respectively, placed using laparoscopic instruments. Groups 3 and 4 used 0 and 2-0 polyglyconate knotless, barbed suture, respectively, placed using an automated laparoscopic suturing device. All groups used a double-layer inverting pattern. Time for suture placement was recorded. Bladders were inflated with water and bursting strength pressures recorded, including a control group of intact bladders. Statistical analysis using a linear model and taking into account the unequal variances was followed by a post-hoc Tukey's test. Significance was set at P<.05.
  
  
  RESULTS
  Bursting strength did not vary significantly between treatment groups, but was significantly decreased compared to the control group (P<.001). Time to place the sutures with the 2 automated suture device groups (groups 3 and 4) was significantly faster than those in which the suture was placed using laparoscopic needle holders and forceps (groups 1 and 2; P=.001).
  
  
  CONCLUSIONS
  Knotless, barbed suture may be a viable alternative to standard suture material for laparoscopic closure of the urinary bladder in horses. Further cyclic and in vivo testing should be performed before use in clinical cases.
authors.affiliation: Department of Clinical Sciences, CHU Sainte-Justine Research Centre, and Research Center of Animal Reproduction, University of Montreal, St. Hyacinthe, Quebec, Canada.
authors.forename: Pavlina
authors.initials: P
authors.lastname: Ruzickova
authors.affiliation: Department of Clinical Sciences, CHU Sainte-Justine Research Centre, and Research Center of Animal Reproduction, University of Montreal, St. Hyacinthe, Quebec, Canada.
authors.forename: Patrick
authors.initials: P
authors.lastname: Burns
authors.affiliation: Department of Clinical Sciences, CHU Sainte-Justine Research Centre, and Research Center of Animal Reproduction, University of Montreal, St. Hyacinthe, Quebec, Canada.
authors.forename: Perrine
authors.initials: P
authors.lastname: Piat
authors.affiliation: Departments of Obstetrics and Gynecology, and Neuroscience, CHU Sainte-Justine Research Centre, and Research Center of Animal Reproduction, University of Montreal, St. Hyacinthe, Quebec, Canada.
authors.forename: Martin G
authors.initials: MG
authors.lastname: Frasch
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, CHU Sainte-Justine Research Centre, and Research Center of Animal Reproduction, University of Montreal, St. Hyacinthe, Quebec, Canada.
authors.forename: Guy
authors.initials: G
authors.lastname: Beauchamp
authors.affiliation: Department of Clinical Sciences, CHU Sainte-Justine Research Centre, and Research Center of Animal Reproduction, University of Montreal, St. Hyacinthe, Quebec, Canada.
authors.forename: Yvonne A
authors.initials: YA
authors.lastname: Elce
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002102:Cadaver; D006736:Horses; D010535:Laparoscopy / Q000662:veterinary; D013536:Suture Techniques / Q000662:veterinary*; D001743:Urinary Bladder / Q000601:surgery*; D013520:Urologic Surgical Procedures / Q000662:veterinary*