title: Mapping phase diagrams of supported lipid bilayers by atomic force microscopy.
abstract: In this work, we present the method followed to construct a pseudophase diagram of two phospholipids: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol). Two different techniques, DSC and AFM, have been used based in the determination of the onset (Tonset) and completion (Toffset) temperatures of the gel-to-liquid crystalline phases (Lβ→Lα), the first from the endotherms from liposomes and the second from the topographic images of supported lipid bilayers. The features of both phase diagrams are discussed emphasizing the influence of Ca2+presence and the substrate (mica) on the transition undergone by the phospholipid mixture. Microsc. Res. Tech. 80:4-10, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Departament de Fisicoquímica, Facultat de Farmàcia and Institut de Nanociència i Nanotecnologia (IN2UB), Universitat de Barcelona (UB), Barcelona, Catalonia, 08028, Spain.
authors.forename: Jordi H
authors.initials: JH
authors.lastname: Borrell
authors.affiliation: Departament de Fisicoquímica, Facultat de Farmàcia and Institut de Nanociència i Nanotecnologia (IN2UB), Universitat de Barcelona (UB), Barcelona, Catalonia, 08028, Spain.
authors.forename: M Teresa
authors.initials: MT
authors.lastname: Montero
authors.affiliation: Departament de Fisicoquímica, Facultat de Farmàcia and Institut de Nanociència i Nanotecnologia (IN2UB), Universitat de Barcelona (UB), Barcelona, Catalonia, 08028, Spain.
authors.forename: Òscar
authors.initials: Ò
authors.lastname: Domènech
mesh_terms: D002152:Calorimetry, Differential Scanning; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry; D018625:Microscopy, Atomic Force; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry
chemical_list: D008051:Lipid Bilayers; D008081:Liposomes; D010714:Phosphatidylethanolamines; D010743:Phospholipids; C000608529:1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine
keywords: AFM; phase diagrams; phospholipids