title: Identification of catecholamine neurotransmitters using fluorescence sensor array.
abstract: A nano-based sensor array has been developed for identification and discrimination of catecholamine neurotransmitters based on optical properties of their oxidation products under alkaline conditions. To produce distinct fluorescence response patterns for individual catecholamine, quenching of thioglycolic acid functionalized cadmium telluride (CdTe) quantum dots, by oxidation products, were employed along with the variation of fluorescence spectra of oxidation products. The spectral changes were analyzed with hierarchical cluster analysis (HCA) and principal component analysis (PCA) to identify catecholamine patterns. The proposed sensor could efficiently discriminate the individual catecholamine (i.e., dopamine, norepinephrine, and l-DOPA) and their mixtures in the concentration range of 0.25-30 μmol L(-1). Finally, we found that the sensor had capability to identify the various catecholamines in urine sample.
authors.affiliation: Department of Chemistry, Sharif University of Technology, Tehran 11155-9516, Iran.
authors.forename: Forough
authors.initials: F
authors.lastname: Ghasemi
authors.affiliation: Department of Chemistry, Sharif University of Technology, Tehran 11155-9516, Iran; Institute for Nanoscience and Nanotechnology, Sharif University of Technology, Tehran, Iran. Electronic address: hormozi@sharif.edu.
authors.forename: M Reza
authors.initials: MR
authors.lastname: Hormozi-Nezhad
authors.affiliation: Department of Nanotechnology and Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 13169-43551, Iran; Division of Cardiovascular Medicine, Stanford University School of Medicine, Stanford, CA 94305-5101, United States. Electronic address: mahmoudi@stanford.edu.
authors.forename: Morteza
authors.initials: M
authors.lastname: Mahmoudi
mesh_terms: D002395:Catecholamines / Q000032:analysis* / Q000652:urine; D005453:Fluorescence; D006801:Humans; D018377:Neurotransmitter Agents / Q000032:analysis* / Q000652:urine; D025341:Principal Component Analysis; D045663:Quantum Dots
chemical_list: D002395:Catecholamines; D018377:Neurotransmitter Agents
keywords: Catecholamine neurotransmitters; Fluorescence; Oxidized catecholamines; Quantum dots; Sensor array