title: Hydrogel coated mesh decreases tissue reaction resulting from polypropylene mesh implant: implication in hernia repair.
abstract: Use of polypropylene mesh (PPM) in hernia repair is associated with tissue reactivity. We examined, in a rat model, a novel non-biodegradable hydrogel coated PPM which may allow for decreased inflammation and a decreased foreign body reaction.
  
  
  
  Through a dorsal midline incision, a 2 cm × 2 cm section of PPM (either coated or uncoated) was placed on the fascial surface 1.5 cm from the incision on the dorsal wall of Sprague-Dawley rats. At 2 and 12 weeks after placement, the PPM and surrounding tissue were harvested. A board-certified dermatopathologist examined H&E stained slides for fibrosis and foreign body reaction. In addition, tissues were stained for apoptotic cells, oxidative damage, macrophages, fibroblasts, neovascularization and metalloproteases.
  
  
  
  At 2 and 12 weeks, there was a greater than 95 % decrease in foreign body giant cells in coated PPM samples compared to uncoated; fibrosis was decreased by 50 %. At 2 and 12 weeks, oxidative damage, fibroblast accumulation, apoptosis and macrophages were significantly decreased in coated PPM samples compared to uncoated PPM.
  
  
  
  These results demonstrate that a non-biodegradable hydrogel coating of PPM led to significant reduction in foreign body reaction, oxidative stress and apoptosis compared to uncoated PPM in vivo, and suggest that this coating could be clinically useful in hernia repair.
authors.affiliation: Department of Urology, Institute for Pediatric Urology, Weill Cornell Medical College, Box 94, 1300 York Avenue, New York, NY, 10065, USA.
authors.forename: D P
authors.initials: DP
authors.lastname: Poppas
authors.affiliation: Department of Pathology, Weill Cornell Medical College, New York, NY, USA.
authors.forename: J J
authors.initials: JJ
authors.lastname: Sung
authors.affiliation: Department of Pathology, Weill Cornell Medical College, New York, NY, USA.
authors.forename: C M
authors.initials: CM
authors.lastname: Magro
authors.affiliation: Department of Urology, Institute for Pediatric Urology, Weill Cornell Medical College, Box 94, 1300 York Avenue, New York, NY, 10065, USA.
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Pathology, Weill Cornell Medical College, New York, NY, USA.
authors.forename: J P
authors.initials: JP
authors.lastname: Toyohara
authors.affiliation: Department of General Surgery, University of Missouri School of Medicine, Columbia, MO, USA.
authors.forename: B J
authors.initials: BJ
authors.lastname: Ramshaw
authors.affiliation: Department of Urology, Institute for Pediatric Urology, Weill Cornell Medical College, Box 94, 1300 York Avenue, New York, NY, 10065, USA. dfelsen@med.cornell.edu.
authors.forename: D
authors.initials: D
authors.lastname: Felsen
mesh_terms: D000818:Animals; D017209:Apoptosis; D020099:Coated Materials, Biocompatible; D004195:Disease Models, Animal; D005549:Foreign-Body Reaction / Q000503:physiopathology*; D059685:Herniorrhaphy / Q000379:methods*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D007249:Inflammation / Q000503:physiopathology*; D007813:Laparotomy; D008297:Male; D008422:Materials Testing; D018384:Oxidative Stress; D011126:Polypropylenes; D019736:Prostheses and Implants; D051381:Rats; D017207:Rats, Sprague-Dawley; D013526:Surgical Mesh; D000072836:Surgical Wound / Q000503:physiopathology*
chemical_list: D020099:Coated Materials, Biocompatible; D011126:Polypropylenes; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate
keywords: Foreign body reaction; Hernia repair; Hydrogel; Polypropylene mesh