title: Antimicrobial activity of the pygidial gland secretion of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) (Insecta: Coleoptera: Carabidae).
abstract: The antimicrobial activity of the pygidial gland secretion released by adult individuals of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828), applying microdilution method with the aim to detect minimal inhibitory concentration, minimal bactericidal concentration and minimal fungicidal concentration, has been investigated. In addition, morphology of the pygidial glands is observed. We have tested 16 laboratory and clinical strains of human pathogens - eight bacterial both gram-positive and gram-negative species and eight fungal species. The pygidial secretion samples have showed antimicrobial properties against all strains of treated bacteria and fungi. Micrococcus flavus proved to be more resistant compared with other bacterial strains. More significant antimicrobial properties of the secretion are observed against Escherichia coli, which proved to be the most sensitive bacteria. Aspergillus fumigatus proved to be the most resistant, while Penicillium ochrochloron and Penicillium verrucosum var. cyclopium the most sensitive micromycetes. Commercial antibiotics Streptomycin and Ampicillin and antimycotics Ketoconazole and Bifonazole, applied as positive controls, showed higher antibacterial properties for all bacterial and fungal strains, except for P. ochrochloron, which proved to be more resistant on Ketoconazole compared with the pygidial gland secretion of L. (P.) punctatus. Apart from the role in ecological aspects, the antimicrobial properties of the tested secretion possibly might have medical significance in the future.
authors.affiliation: Institute of Zoology,University of Belgrade - Faculty of Biology,Studentski Trg 16,11000 Belgrade,Serbia.
authors.forename: M
authors.initials: M
authors.lastname: Nenadić
authors.affiliation: Institute for Biological Research 'Siniša Stanković',University of Belgrade,Bulevar Despota Stefana 142,11060 Belgrade,Serbia.
authors.forename: M
authors.initials: M
authors.lastname: Soković
authors.affiliation: Institute for Biological Research 'Siniša Stanković',University of Belgrade,Bulevar Despota Stefana 142,11060 Belgrade,Serbia.
authors.forename: J
authors.initials: J
authors.lastname: Glamočlija
authors.affiliation: Institute for Biological Research 'Siniša Stanković',University of Belgrade,Bulevar Despota Stefana 142,11060 Belgrade,Serbia.
authors.forename: A
authors.initials: A
authors.lastname: Ćirić
authors.affiliation: Institute for Biological Research 'Siniša Stanković',University of Belgrade,Bulevar Despota Stefana 142,11060 Belgrade,Serbia.
authors.forename: V
authors.initials: V
authors.lastname: Perić-Mataruga
authors.affiliation: University of Belgrade - Faculty of Chemistry,Studentski Trg 12-16,11000 Belgrade,Serbia.
authors.forename: V
authors.initials: V
authors.lastname: Tešević
authors.affiliation: University of Belgrade - Faculty of Chemistry,Studentski Trg 12-16,11000 Belgrade,Serbia.
authors.forename: L
authors.initials: L
authors.lastname: Vujisić
authors.affiliation: University of Belgrade - Faculty of Chemistry,Studentski Trg 12-16,11000 Belgrade,Serbia.
authors.forename: M
authors.initials: M
authors.lastname: Todosijević
authors.affiliation: Institute of Zoology,University of Belgrade - Faculty of Biology,Studentski Trg 16,11000 Belgrade,Serbia.
authors.forename: N
authors.initials: N
authors.lastname: Vesović
authors.affiliation: Institute of Zoology,University of Belgrade - Faculty of Biology,Studentski Trg 16,11000 Belgrade,Serbia.
authors.forename: S
authors.initials: S
authors.lastname: Ćurčić
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D001517:Coleoptera / Q000737:chemistry*; D005658:Fungi / Q000187:drug effects*; D008826:Microbial Sensitivity Tests
chemical_list: D000890:Anti-Infective Agents
keywords: Laemostenus (Pristonychus) punctatus; antibacterial and antifungal activity; human pathogens; microdilution; morphology; pygidial gland secretion