title: Exogenous Neuritin Promotes Nerve Regeneration After Acute Spinal Cord Injury in Rats.
abstract: Insufficient local levels of neurotrophic factor after spinal cord injury (SCI) are the leading cause of secondary injury and limited axonal regeneration. Neuritin belongs to a family of neurotrophic factors that promote neurite outgrowth, maintain neuronal survival, and provide a favorable microenvironment for the regeneration and repair of nerve cells after injury. However, it is not known whether the exogenously applied neuritin protein has a positive effect on nerve repair after SCI. This was investigated in the present study using purified human recombinant neuritin expressed in and purified from Pichia pastoris, which was tested in a rat SCI model. A recombinant neuritin concentration of 60 μg/ml induced the recovery of hind limb motor function and stimulated nerve regeneration in rats with SCI. Continuous administration of neuritin at this dose at an early stage after SCI inhibited poly ADP ribose polymerase (PARP) protein degradation and decreased neuronal apoptosis. In addition, during the critical postinjury period of axonal regeneration, exogenous neuritin treatment increased the expression of neurofilament 200 and growth-associated protein 43 in the damaged tissue, which was associated with the restoration of hind limb movement. These results suggest that neuritin creates an environment that promotes nerve cell survival and neurite regeneration after SCI, which contribute to nerve regeneration and the recovery of motor function.
authors.affiliation: 1 The Key Laboratory of Xinjiang Endemic & Ethnic Diseases and Department of Biochemistry, Shihezi University School of Medicine, Shihezi, Xinjiang, China .
authors.forename: Rui
authors.initials: R
authors.lastname: Gao
authors.affiliation: 1 The Key Laboratory of Xinjiang Endemic & Ethnic Diseases and Department of Biochemistry, Shihezi University School of Medicine, Shihezi, Xinjiang, China .
authors.forename: Xingyi
authors.initials: X
authors.lastname: Li
authors.affiliation: 1 The Key Laboratory of Xinjiang Endemic & Ethnic Diseases and Department of Biochemistry, Shihezi University School of Medicine, Shihezi, Xinjiang, China .
authors.forename: Shaosong
authors.initials: S
authors.lastname: Xi
authors.affiliation: 1 The Key Laboratory of Xinjiang Endemic & Ethnic Diseases and Department of Biochemistry, Shihezi University School of Medicine, Shihezi, Xinjiang, China .
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
authors.affiliation: 1 The Key Laboratory of Xinjiang Endemic & Ethnic Diseases and Department of Biochemistry, Shihezi University School of Medicine, Shihezi, Xinjiang, China .
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: 1 The Key Laboratory of Xinjiang Endemic & Ethnic Diseases and Department of Biochemistry, Shihezi University School of Medicine, Shihezi, Xinjiang, China .
authors.forename: Jingling
authors.initials: J
authors.lastname: Zhu
authors.affiliation: 1 The Key Laboratory of Xinjiang Endemic & Ethnic Diseases and Department of Biochemistry, Shihezi University School of Medicine, Shihezi, Xinjiang, China .
authors.forename: Liya
authors.initials: L
authors.lastname: Shan
authors.affiliation: 2 School of Medicine & Health Management, Hangzhou Normal University , Hangzhou, Zhejiang, China .
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Song
authors.affiliation: 1 The Key Laboratory of Xinjiang Endemic & Ethnic Diseases and Department of Biochemistry, Shihezi University School of Medicine, Shihezi, Xinjiang, China .
authors.forename: Xing
authors.initials: X
authors.lastname: Luo
authors.affiliation: 2 School of Medicine & Health Management, Hangzhou Normal University , Hangzhou, Zhejiang, China .
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: 1 The Key Laboratory of Xinjiang Endemic & Ethnic Diseases and Department of Biochemistry, Shihezi University School of Medicine, Shihezi, Xinjiang, China .
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
mesh_terms: D000818:Animals; D001369:Axons / Q000187:drug effects / Q000502:physiology*; D005260:Female; D058851:GPI-Linked Proteins / Q000494:pharmacology; D005822:Genetic Vectors / Q000008:administration & dosage*; D008297:Male; D009416:Nerve Regeneration / Q000187:drug effects*; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D009479:Neuropeptides / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function; D013116:Spinal Cord / Q000166:cytology* / Q000378:metabolism; D013119:Spinal Cord Injuries / Q000503:physiopathology*
chemical_list: D058851:GPI-Linked Proteins; D009479:Neuropeptides; C105161:Nrn1 protein, rat