title: Ultrasound-assisted magnetic dispersive solid-phase microextraction: A novel approach for the rapid and efficient microextraction of naproxen and ibuprofen employing experimental design with high-performance liquid chromatography.
abstract: A simple, rapid, and sensitive method for the determination of naproxen and ibuprofen in complex biological and water matrices (cow milk, human urine, river, and well water samples) has been developed using ultrasound-assisted magnetic dispersive solid-phase microextraction. Magnetic ethylendiamine-functionalized graphene oxide nanocomposite was synthesized and used as a novel adsorbent for the microextraction process and showed great adsorptive ability toward these analytes. Different parameters affecting the microextraction were optimized with the aid of the experimental design approach. A Plackett-Burman screening design was used to study the main variables affecting the microextraction process, and the Box-Behnken optimization design was used to optimize the previously selected variables for extraction of naproxen and ibuprofen. The optimized technique provides good repeatability (relative standard deviations of the intraday precision 3.1 and 3.3, interday precision of 5.6 and 6.1%), linearity (0.1-500 and 0.3-650 ng/mL), low limits of detection (0.03 and 0.1 ng/mL), and a high enrichment factor (168 and 146) for naproxen and ibuprofen, respectively. The proposed method can be successfully applied in routine analysis for determination of naproxen and ibuprofen in cow milk, human urine, and real water samples.
authors.affiliation: Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Ghorbani
authors.affiliation: Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Chamsaz
authors.affiliation: Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Gholam Hossein
authors.initials: GH
authors.lastname: Rounaghi
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004867:Equipment Design; D006801:Humans; D007052:Ibuprofen / Q000302:isolation & purification*; D008280:Magnetics; D009288:Naproxen / Q000302:isolation & purification*; D052617:Solid Phase Microextraction / Q000379:methods*; D014465:Ultrasonics; D014874:Water Pollutants, Chemical / Q000302:isolation & purification
chemical_list: D014874:Water Pollutants, Chemical; D009288:Naproxen; D007052:Ibuprofen
keywords: Box-Behnken design; Dispersive solid-phase microextraction; Graphene oxide nanocomposites; Plackett-Burman design