title: Anxiety-related behavioral responses of pentylenetetrazole-treated zebrafish larvae to light-dark transitions.
abstract: Pentylenetetrazole (PTZ), γ-aminobutyrate (GABA) antagonist, is a convulsant drug, known to induce anxiety and seizures in zebrafish. Changes in the mobility of zebrafish under light-dark transitions reflect anxiety level, serving as a useful behavioral readout. The effects of PTZ treatment have yet to be assayed in this manner. Zebrafish larvae (AB strain) at both 5dpf (days post-fertilization) and 7dpf were treated with different concentrations of PTZ. General locomotor activity and thigmotaxis were analyzed under continuous illumination (normal conditions) or alternating light-dark cycles (stressful conditions). Zebrafish larvae of 5dpf and 7dpf exhibited different sensitivities to PTZ. Anxiety level, measured in terms of response to illumination transitions under the influence of PTZ, demonstrated contrasting tendencies. Dark-light transitions dramatically increased the locomotor activity of zebrafish larvae receiving 8mM PTZ which was indicative of anxiety. This study suggests that PTZ increases the susceptibility by activating the neuron, which perhaps makes light change easier to influence the anxiety level of larvae. We provide useful evidence for putative anti-anxiety drug screening.
authors.affiliation: Translational Medical Center for Development and Disease, Shanghai Key Laboratory of Birth Defect, Institute of Pediatrics, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai 201102, China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Peng
authors.affiliation: Translational Medical Center for Development and Disease, Shanghai Key Laboratory of Birth Defect, Institute of Pediatrics, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai 201102, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Lin
authors.affiliation: Translational Medical Center for Development and Disease, Shanghai Key Laboratory of Birth Defect, Institute of Pediatrics, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai 201102, China.
authors.forename: Yingdong
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Translational Medical Center for Development and Disease, Shanghai Key Laboratory of Birth Defect, Institute of Pediatrics, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai 201102, China.
authors.forename: Xiuyun
authors.initials: X
authors.lastname: Liu
authors.affiliation: Translational Medical Center for Development and Disease, Shanghai Key Laboratory of Birth Defect, Institute of Pediatrics, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai 201102, China.
authors.forename: Yinglan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Translational Medical Center for Development and Disease, Shanghai Key Laboratory of Birth Defect, Institute of Pediatrics, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai 201102, China.
authors.forename: Yongxia
authors.initials: Y
authors.lastname: Ji
authors.affiliation: Translational Medical Center for Development and Disease, Shanghai Key Laboratory of Birth Defect, Institute of Pediatrics, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai 201102, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Yang
authors.affiliation: Key Laboratory of Molecular Virology and Immunology, Institute Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200025, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Center for Chinese Medical Therapy and Systems Biology, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, China. Electronic address: gn@shutcm.edu.cn.
authors.forename: Ning
authors.initials: N
authors.lastname: Guo
authors.affiliation: Translational Medical Center for Development and Disease, Shanghai Key Laboratory of Birth Defect, Institute of Pediatrics, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai 201102, China. Electronic address: liq@fudan.edu.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
mesh_terms: D000818:Animals; D001007:Anxiety / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D006854:Hydrocortisone / Q000378:metabolism; D007814:Larva / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D008124:Locomotion / Q000187:drug effects*; D008297:Male; D010433:Pentylenetetrazole / Q000494:pharmacology* / Q000627:therapeutic use; D017440:Photoperiod; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D015027:Zebrafish / Q000254:growth & development / Q000502:physiology*
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D006854:Hydrocortisone; D010433:Pentylenetetrazole
keywords: Anxiety; Light-dark transition; Locomotion; Pentylenetetrazole; Thigmotaxis