title: Cobalt, titanium and PMMA bone cement debris influence on mouse osteoblast cell elasticity, spring constant and calcium production activity.
abstract: Periprosthetic osteolysis and implant loosening are the outcomes of wear debris generation in total joint replacements. Wear debris formed from the implanted materials consisting of metals, polymers, ceramic and bone cement initiate the immune system response. Often osteoblasts, the principal cell type in bone tissue adjacent to the prostheses, are directly impacted. In this study, the influence of cobalt, titanium and PMMA bone cement particles of different sizes, charges and compositions on mouse osteoblast adhesion, nanomechanics (elasticity and spring constant) and metabolic activity were investigated. These studies were accompanied by osteoblast mineralisation experiments and cell uptake after exposure to particles at defined time points. Our results demonstrate that alteration of the nanomechanical properties are mainly dependent on the metal type rather than nanoparticles size and concentration. Moreover, despite uptake increasing over exposure time, the cell characteristics exhibit changes predominately after the first 24 hours, highlighting that the cell responses to nanoparticle exposure are not cumulative. Understanding these processes is critical to expanding our knowledge of implant loosening and elucidating the nature of prosthetic joint failure.
authors.affiliation: School of Pharmacy and Pharmaceutical Sciences, Cardiff University, Cardiff, UK. Email: prokopovichp@cardiff.ac.uk ; ; Tel: +44 (0)29 208 75820.
authors.forename: Emily Callard
authors.initials: EC
authors.lastname: Preedy
authors.affiliation: School of Pharmacy and Pharmaceutical Sciences, Cardiff University, Cardiff, UK. Email: prokopovichp@cardiff.ac.uk; ; Tel: +44 (0)29 208 75820; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, USA.
authors.forename: Stefano
authors.initials: S
authors.lastname: Perni
authors.affiliation: School of Pharmacy and Pharmaceutical Sciences, Cardiff University, Cardiff, UK. Email: prokopovichp@cardiff.ac.uk; ; Tel: +44 (0)29 208 75820; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, USA.
authors.forename: Polina
authors.initials: P
authors.lastname: Prokopovich