title: High Risk HPV E6/E7 Oncoprotein Expression in Women with High Grade Squamous Intraepithelial Lesion.
abstract: OBJECTIVE
  To correlate the expression of high-risk HPV E6 mRNA with pap smear, colposcopy, and biopsy results in women with high grade squamous intraepithelial lesion (HSIL).
  
  
  METHODS
  A cross-sectional study was performed on women referred for primary care services after cytological diagnosis of HSIL. We evaluated the expression of E6/E7 mRNA of HPV types 16,18,31,33, and 45 and correlated the results with those of Pap smear, colposcopy, and biopsy. For amplification/detection of mRNA E6 / E7 we used NucliSENSEasyQ kit to detect HPV mRNA by polymerase chain reaction with primers/probes for HPV types 16, 18, 31, 33, and 45.
  
  
  RESULTS
  Out of 128 valid tests, the results of 30 (23.4%) tests were negative and 98 (70%) tests were positive. Only one type of HPV was detected in 87.7% of the E6/E7 mRNA positive cases. HPV16 was detected in 61.2% of the cases, followed by HPV33 (26.5%), HPV31 (17.3%), HPV18 (10%), and HPV45 (4.08%). Pap smear tests revealed that the E6/E7 test was positive in 107 (83.8%) women with atypical squamous cells - high grade (ASC-H), HSIL, or higher. The E6/E7 test was positive in 69 (57.5%) specimens presenting negative cytology results. When analyzing the association with colposcopy results, the frequency of positive E6/E7 results increased with the severity of the injury, ranging from 57.1% in women without colposcopy-detected injury to 86.5% in those with higher levels of colposcopy findings. Of the 111 women who underwent biopsy and E6/E7 testing, the E6/E7 test was positive in 84.7% of the women who presented with lesions of cervical intraepithelial neoplasia (CIN) grade 2 or higher. Finally, 41.2% of women with a negative biopsy presented a positive E6/E7 test.
  
  
  CONCLUSIONS
  E6/E7 mRNA expression was higher in women with HSIL and CIN grade 2 or higher.
authors.affiliation: Gynecology and Obstetric Department, Universidade Federal de Pernambuco - UFPE, Recife, PE, Brazil.
authors.forename: Jefferson Elias Cordeiro
authors.initials: JE
authors.lastname: Valença
authors.affiliation: Gynecology and Obstetric Department, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Ana Katherine
authors.initials: AK
authors.lastname: Gonçalves
authors.affiliation: Gynecology and Obstetric Department, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Ismael Dale Cotrim
authors.initials: ID
authors.lastname: Guerreiro da Silva
authors.affiliation: Gynecology and Obstetric Department, Universidade Federal do Ceará, Fortaleza, CE, Brazil.
authors.forename: José
authors.initials: J
authors.lastname: Eleutério Junior
authors.affiliation: Gynecology and Obstetric Department, Universidade Federal de Pernambuco - UFPE, Recife, PE, Brazil.
authors.forename: Terezinha
authors.initials: T
authors.lastname: Tenório da Silva
authors.affiliation: Biochemistry Department, UFPE, Recife, PE, Brazil.
authors.forename: Danyelly
authors.initials: D
authors.lastname: Bruneska
authors.affiliation: Tropical Medicine Department, UFPE, Recife, PE, Brazil.
authors.forename: Ricardo Arraes de Alencar
authors.initials: RA
authors.lastname: Ximenes
mesh_terms: D000328:Adult; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D015513:Oncogene Proteins; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D027383:Papillomaviridae; D030361:Papillomavirus Infections / Q000175:diagnosis; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D065310:Squamous Intraepithelial Lesions of the Cervix / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis; D014626:Vaginal Smears; D002578:Uterine Cervical Dysplasia / Q000235:genetics*
chemical_list: D015513:Oncogene Proteins; D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger