title: Toward DNA electrochemical sensing by free-standing ZnO nanosheets grown on 2D thin-layered MoS2.
abstract: Very recently, the 2-dimensional MoS2layer as base substrate integrated with other materials has caused people's emerging attention. In this paper, a thin-layered MoS2was prepared through an ultrasonic exfoliation method from bulk MoS2and then the free-standing ZnO nanosheet was electrodeposited on the MoS2scaffold for DNA sensing. The ZnO/MoS2nanocomposite revealed smooth and vertical nanosheets morphology by scanning electron microscopy, compared with the sole MoS2and sole ZnO. Importantly, the partially negative charged MoS2layer is beneficial to the nucleation and growth of ZnO nanosheets under the effect of electrostatic interactions. Classic methylene blue, which possesses different affinities to dsDNA and ssDNA, was adopted as the measure signal to confirm the immobilization and hybridization of DNA on ZnO nanosheets and pursue the optimal synthetic conditions. And the results demonstrated that the free-standing ZnO/MoS2nanosheets had low detection limit (6.6×10-16M) and has a positive influence on DNA immobilization and hybridization.
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker of Education Ministry, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. Electronic address: taoyang@qust.edu.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker of Education Ministry, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
authors.forename: Meijing
authors.initials: M
authors.lastname: Chen
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker of Education Ministry, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Kong
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker of Education Ministry, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China; Shandong Provincial Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. Electronic address: xiliangluo@hotmail.com.
authors.forename: Xiliang
authors.initials: X
authors.lastname: Luo
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker of Education Ministry, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
authors.forename: Kui
authors.initials: K
authors.lastname: Jiao
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D004247:DNA / Q000032:analysis*; D015342:DNA Probes / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D004595:Electroplating; D061925:Immobilized Nucleic Acids / Q000737:chemistry; D057230:Limit of Detection; D008982:Molybdenum / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D009693:Nucleic Acid Hybridization / Q000379:methods; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D015342:DNA Probes; D004277:DNA, Single-Stranded; D004220:Disulfides; D061925:Immobilized Nucleic Acids; D008982:Molybdenum; D004247:DNA; D015034:Zinc Oxide; C082964:molybdenum disulfide
keywords: DNA; Electrodeposition; MoS(2); Nanosheets; ZnO