title: ZnO-Based Nanoplatforms for Labeling and Treatment of Mouse Tumors without Detectable Toxic Side Effects.
abstract: ZnO quantum dots (QDs) were synthesized with polymer shells, coordinated with Gd(3+) ions and adsorbed doxorubicin (DOX) together to form a new kind of multifunctional ZnO-Gd-DOX nanoplatform. Such pH sensitive nanoplatforms were shown to release DOX to cancer cells in vitro and to mouse tumors in vivo, and reveal better specificity and lower toxicity than free DOX, and even better therapeutic efficacy than an FDA approved commercial DOX-loading drug DOX-Liposome Injection (DOXIL, NDA#050718). The ZnO-Gd-DOX nanoplatforms exhibited strong red fluorescence, which benefited the fluorescent imaging on live mice. Due to the special structure of ZnO-Gd-DOX nanoparticles, such nanoplatforms possessed a high longitudinal relaxivity r1 of 52.5 mM(-1) s(-1) at 0.55 T, which was superior to many other Gd(3+) based nanoparticles. Thus, both fluorescence labeling and magnetic resonance imaging could be applied simultaneously on the tumor bearing mice along with drug delivery. After 36 days of treatment on these mice, ZnO-Gd-DOX nanoparticles greatly inhibited the tumor growth without causing any appreciable abnormality in major organs. The most important merit of ZnO-Gd-DOX was that such a nanoplatform was biodegraded completely and showed no toxic side effects after H&E (hematoxylin and eosin) staining of tumor slices and ICP-AES (inductively coupled plasma atomic emission spectrometry) bioanalyses.
authors.affiliation: Department of Chemistry, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Dai-Xin
authors.initials: DX
authors.lastname: Ye
authors.affiliation: Department of Chemistry, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Ma
authors.affiliation: Department of Chemistry, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Chemistry, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Cao
authors.affiliation: Department of Chemistry, Fudan University , Shanghai 200433, P. R. China.|Institutes of Biomedical Sciences, Fudan University , Shanghai 200032, P. R. China.
authors.forename: Ji-Lie
authors.initials: JL
authors.lastname: Kong
authors.affiliation: Department of Chemistry, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Huan-Ming
authors.initials: HM
authors.lastname: Xiong
authors.affiliation: Max-Planck Institute of Colloids and Interfaces , Potsdam 14424, Germany.
authors.forename: Helmuth
authors.initials: H
authors.lastname: Möhwald
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000009:adverse effects / Q000737:chemistry* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival; D003287:Contrast Media / Q000737:chemistry*; D004317:Doxorubicin / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000737:chemistry / Q000627:therapeutic use; D004337:Drug Carriers / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005682:Gadolinium / Q000737:chemistry*; D006801:Humans; D008279:Magnetic Resonance Imaging; D008689:Methacrylates / Q000737:chemistry; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000000981:diagnostic imaging* / Q000188:drug therapy; D010316:Particle Size; D011092:Polyethylene Glycols / Q000009:adverse effects / Q000737:chemistry / Q000627:therapeutic use; D045663:Quantum Dots / Q000737:chemistry*; D013499:Surface Properties; D014018:Tissue Distribution; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D003287:Contrast Media; D004337:Drug Carriers; D005456:Fluorescent Dyes; D008689:Methacrylates; C506643:liposomal doxorubicin; C524499:polyethylene glycol methacrylate; D011092:Polyethylene Glycols; D004317:Doxorubicin; D005682:Gadolinium; D015034:Zinc Oxide
keywords: ZnO; anticancer; drug delivery; fluorescent imaging; magnetic resonance imaging