title: Protective effects of rosmarinic acid against radiation-induced damage to the hematopoietic system in mice.
abstract: Rosmarinic acid (RA) is an ester of caffeic acid and 3, 4-dihydroxyphenyl lactic acid. It is a potent antioxidant that functions by scavenging free radicals. Here, we used a 30-day survival assay to investigate the radioprotective effects of RA. Mice were treated with RA once per day for 10 consecutive days starting at 3 days before gamma irradiation at 7.5 Gy until 7 days post irradiation. Mice treated with 100 and 200 mg/kg body weight (bw) of RA had 30-day survival rates of 89% and 72%, respectively, compared with 32% in the control group, and the differences were statistically significant (P = 0.0008 and 0.0421, respectively). Spleen colony-forming units (CFU-S), the number of nucleated cells in the bone marrow (BMNC), bone marrow DNA content, and hematological parameters of the peripheral blood were measured to investigate the radioprotective effect of RA on the hematopoietic system. The treatment groups that received RA at 50, 100 and 150 mg/kg bw and whole-body exposure to 5.5 Gy of (137)Cs γ- radiation had significantly higher CFU-S, BMNC and DNA content than the irradiation-only group. Assessment of hematological parameters in the peripheral blood showed that the treatment groups receiving RA at doses of 50, 100 and 150 mg/kg bw had higher white blood cell counts, hemoglobin and platelets than the radiation-only group. These results suggested that the administration of RA promoted the recovery of peripheral blood cells in irradiated mice.
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 238 Bai Di Road, Nan Kai District, Tianjin, 300192, China xuwenqing@irm-cams.ac.cn.
authors.forename: Wenqing
authors.initials: W
authors.lastname: Xu
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 238 Bai Di Road, Nan Kai District, Tianjin, 300192, China.
authors.forename: Fujun
authors.initials: F
authors.lastname: Yang
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 238 Bai Di Road, Nan Kai District, Tianjin, 300192, China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 238 Bai Di Road, Nan Kai District, Tianjin, 300192, China.
authors.forename: Xiu
authors.initials: X
authors.lastname: Shen
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000187:drug effects; D001854:Bone Marrow Cells / Q000528:radiation effects; D002452:Cell Count; D002934:Cinnamates / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*; D003114:Colony-Forming Units Assay; D004247:DNA / Q000378:metabolism; D053630:Depsides / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*; D006413:Hematopoietic System / Q000187:drug effects / Q000473:pathology*; D051379:Mice; D011833:Radiation Injuries, Experimental / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D011837:Radiation-Protective Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*; D013154:Spleen / Q000187:drug effects; D016019:Survival Analysis; D000097123:Rosmarinic Acid
chemical_list: D002934:Cinnamates; D004247:DNA; D053630:Depsides; D011837:Radiation-Protective Agents; D000097123:Rosmarinic Acid
keywords: hematopoietic system; radioprotection; rosmarinic acid