title: Apoptosis inducing ability of silver decorated highly reduced graphene oxide nanocomposites in A549 lung cancer.
abstract: Recently, graphene and graphene-based materials have been increasingly used for various biological applications due to their extraordinary physicochemical properties. Here, we demonstrate the anticancer properties and apoptosis-inducing ability of silver doped highly reduced graphene oxide nanocomposites synthesized by employing green approach. These nano composites (PGE-HRG-Ag) were synthesized by using Pulicaria glutinosa extract (PGE) as a reducing agent and were evaluated for their anticancer properties against various human cancer cell lines with tamoxifen as the reference drug. A correlation between the amount of Ag nanoparticles on the surface of highly reduced graphene oxide (HRG) and the anticancer activity of nanocomposite was observed, wherein an increase in the concentration of Ag nanoparticles on the surface of HRG led to the enhanced anticancer activity of the nanocomposite. The nanocomposite PGE-HRG-Ag-2 exhibited more potent cytotoxicity than standard drug in A549 cells, a human lung cancer cell line. A detailed investigation was undertaken and Fluorescence activated cell sorting (FACS) analysis demonstrated that the nanocomposite PGE-HRG-Ag-2 showed G0/G1 phase cell cycle arrest and induced apoptosis in A549 cells. Studies such as, measurement of mitochondrial membrane potential, generation of reactive oxygen species (ROS) and Annexin V-FITC staining assay suggested that this compound induced apoptosis in human lung cancer cells.
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Merajuddin
authors.initials: M
authors.lastname: Khan
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Mujeeb
authors.initials: M
authors.lastname: Khan
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Abdulhadi H
authors.initials: AH
authors.lastname: Al-Marri
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Abdulrahman
authors.initials: A
authors.lastname: Al-Warthan
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Hamad Z
authors.initials: HZ
authors.lastname: Alkhathlan
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Mohammed Rafiq H
authors.initials: MR
authors.lastname: Siddiqui
authors.affiliation: Department of Medicinal Chemistry and Pharmacology, CSIR - Indian Institute of Chemical Technology, Hyderabad, India.
authors.forename: Vadithe Lakshma
authors.initials: VL
authors.lastname: Nayak
authors.affiliation: Department of Medicinal Chemistry and Pharmacology, CSIR - Indian Institute of Chemical Technology, Hyderabad, India.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Kamal
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Syed F
authors.initials: SF
authors.lastname: Adil
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D053761:Nanocomposites / Q000008:administration & dosage / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D052587:Pulicaria / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism; D012834:Silver / Q000737:chemistry*; D014407:Tumor Cells, Cultured
chemical_list: D010087:Oxides; D017382:Reactive Oxygen Species; D012834:Silver; D006108:Graphite
keywords: anticancer; apoptosis; graphene/silver nanocomposites; plant extract