title: NMR studies on mechanism of isomerisation of fructose 6-phosphate to glucose 6-phosphate catalysed by phosphoglucose isomerase from Thermococcus kodakarensis.
abstract: The fate of hydrogen atoms at C-2 of glucose 6-phosphate (G6P) and C-1 of fructose 6-phosphate (F6P) was studied in the reaction catalysed by phosphoglucose isomerase from Thermococcus kodakarensis (TkPGI) through 1D and 2D NMR methods. When the reaction was performed in (2)H2O the hydrogen atoms in the aforementioned positions were exchanged with deuterons indicating that the isomerization occurred by a cis-enediol intermediate involving C-1 pro-R hydrogen of F6P. These features are similar to those described for phosphoglucose isomerases from rabbit muscle and Pyrococcus furiosus.
authors.affiliation: School of Biological Sciences, University of the Punjab, Lahore, Pakistan; Department of Biology, Lahore Garrison University, DHA, Lahore, Pakistan.
authors.forename: Shahzada Nadeem
authors.initials: SN
authors.lastname: Abbas
authors.affiliation: School of Biochemistry and Immunology, Trinity College, University of Dublin, Ireland.
authors.forename: Kenneth Hun
authors.initials: KH
authors.lastname: Mok
authors.affiliation: School of Biological Sciences, University of the Punjab, Lahore, Pakistan.
authors.forename: Naeem
authors.initials: N
authors.lastname: Rashid
authors.affiliation: School of Biochemistry and Immunology, Trinity College, University of Dublin, Ireland.
authors.forename: Yongjing
authors.initials: Y
authors.lastname: Xie
authors.affiliation: School of Chemistry, Trinity College, University of Dublin, Ireland.
authors.forename: Manuel
authors.initials: M
authors.lastname: Ruether
authors.affiliation: School of Chemistry, Trinity College, University of Dublin, Ireland.
authors.forename: John
authors.initials: J
authors.lastname: O'Brien
authors.affiliation: School of Biological Sciences, University of the Punjab, Lahore, Pakistan. Electronic address: ma3@soton.ac.uk.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Akhtar
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D005636:Fructosephosphates / Q000737:chemistry / Q000378:metabolism*; D019298:Glucose-6-Phosphate / Q000737:chemistry / Q000378:metabolism*; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D011817:Rabbits; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D019712:Thermococcus / Q000201:enzymology*
chemical_list: D005636:Fructosephosphates; D019298:Glucose-6-Phosphate; C027618:fructose-6-phosphate; D005956:Glucose-6-Phosphate Isomerase
keywords: Hyperthermophilic; Isomerization; Mechanism; Nuclear magnetic resonance; Phosphoglucose isomerase; Thermococcus kodakarensis