title: Functional proteomics within the genus Lactobacillus.
abstract: Lactobacillus are mainly used for the manufacture of fermented dairy, sourdough, meat, and vegetable foods or used as probiotics. Under optimal processing conditions, Lactobacillus strains contribute to food functionality through their enzyme portfolio and the release of metabolites. An extensive genomic diversity analysis was conducted to elucidate the core features of the genus Lactobacillus, and to provide a better comprehension of niche adaptation of the strains. However, proteomics is an indispensable "omics" science to elucidate the proteome diversity, and the mechanisms of regulation and adaptation of Lactobacillus strains. This review focuses on the novel and comprehensive knowledge of functional proteomics and metaproteomics of Lactobacillus species. A large list of proteomic case studies of different Lactobacillus species is provided to illustrate the adaptability of the main metabolic pathways (e.g., carbohydrate transport and metabolism, pyruvate metabolism, proteolytic system, amino acid metabolism, and protein synthesis) to various life conditions. These investigations have highlighted that lactobacilli modulate the level of a complex panel of proteins to growth/survive in different ecological niches. In addition to the general regulation and stress response, specific metabolic pathways can be switched on and off, modifying the behavior of the strains.
authors.affiliation: Department of Soil, Plant and Food Science, University of Bari Aldo Moro, Bari, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: De Angelis
authors.affiliation: Department of Soil, Plant and Food Science, University of Bari Aldo Moro, Bari, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Calasso
authors.affiliation: Department of Soil, Plant and Food Science, University of Bari Aldo Moro, Bari, Italy.
authors.forename: Noemi
authors.initials: N
authors.lastname: Cavallo
authors.affiliation: Department of Soil, Plant and Food Science, University of Bari Aldo Moro, Bari, Italy.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Di Cagno
authors.affiliation: Department of Soil, Plant and Food Science, University of Bari Aldo Moro, Bari, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Gobbetti
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D007778:Lactobacillus / Q000378:metabolism* / Q000502:physiology; D053858:Metabolic Networks and Pathways; D020543:Proteome / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction
chemical_list: D001426:Bacterial Proteins; D020543:Proteome
keywords: Environmental metabolic adaptation; Lactobacillus; Microbiology; Secretome; Stress response