title: Liposomes containing recombinant E protein vaccine against duck Tembusu virus in ducks.
abstract: To obtain an effective vaccine candidate against duck Tembusu viral (DTMUV) disease which causes egg-drop and great economical loss in the Chinese duck industry, liposome vaccines containing recombinant E protein were prepared and assessed in this study. The recombinant plasmid (PET28a-E) was constructed and transformed into BL21 (DE3) cells to produce E proteins. The recombinant E proteins were purified and entrapped by liposomes through reverse-phase evaporation. Eighty-four cherry valley ducks were randomly divided into seven groups and inoculated intramuscularly at one- or seven-day-old with liposomes-E protein or Freund's adjuvant-E protein vaccine. Blood samples were collected from the first week to the tenth week for serum antibody, plasma for viremia, as well as oropharyngeal and cloacal swabs for virus shedding analyses after being challenged with a 10(2.4) 50% tissue culture infective dose (TCID50) of duck Tembusu virus. Results showed that serum antibody level of the liposomes vaccine was higher than the Freund's adjuvant vaccine, and inoculating twice was superior to once; furthermore, the viremia and virus shedding tests also proved that the liposomes vaccine can provide complete protection against DTMUV challenge. These results demonstrated that the liposomes-E protein vaccine could be used as a potential candidate vaccine to prevent DTMUV infection in ducks.
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.
authors.forename: Tengfei
authors.initials: T
authors.lastname: Ma
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.
authors.forename: Yongxia
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Cheng
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.
authors.forename: Yanhan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Fan
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.
authors.forename: Ziqiang
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Niu
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China. Electronic address: Liujz@sdau.edu.cn.
authors.forename: Jianzhu
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D004372:Ducks; D005416:Flavivirus; D005620:Freund's Adjuvant / Q000008:administration & dosage; D056724:Immunity, Humoral; D008081:Liposomes / Q000737:chemistry*; D011201:Poultry Diseases / Q000517:prevention & control*; D011897:Random Allocation; D011994:Recombinant Proteins / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*; D017201:Virus Shedding
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D008081:Liposomes; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C065696:glycoprotein E, Flavivirus; D005620:Freund's Adjuvant
keywords: Antibody; DTMUV; E protein; Liposome vaccine; Viremia