title: Lack of detection of human papillomavirus DNA in prostate carcinomas in patients from northeastern Brazil.
abstract: Prostate cancer is the second most common cancer among men in western populations, and despite its high mortality, its etiology remains unknown. Inflammatory processes are related to the etiology of various types of tumors, and prostate inflammation, in particular, has been associated with prostate cancer carcinogenesis and progression. Human papillomavirus (HPV) is associated with benign and malignant lesions in the anogenital tract of both females and males. The possible role of HPV in prostate carcinogenesis is a subject of great controversy. In this study, we aimed to examine the prevalence of HPV infections in prostate carcinomas of patients from northeastern Brazil. This study included 104 tissue samples from primary prostate carcinoma cases. HPV DNA was purified and then amplified using MY09/11 and GP5+/GP6+ degenerate primer sets that detect a wide range of HPV types, and with specific PCR primers sets for E6 and E7 HPV regions to detect HPV 16. None of the samples showed amplification products of HPV DNA for primer sets MY09/11 and GP5+/GP6+, or the specific primer set for the E6 and E7 HPV regions. HPV infection, thus, does not seem to be one of the causes of prostate cancer in the population studied.
authors.affiliation: Laboratório de Genética e Biologia Molecular, Universidade Federal de Piauí, Parnaíba, PI, Brazil.
authors.forename: Ari P
authors.initials: AP
authors.lastname: Araujo-Neto
authors.affiliation: Laboratório de Genética e Biologia Molecular, Universidade Federal de Piauí, Parnaíba, PI, Brazil.
authors.forename: Hygor
authors.initials: H
authors.lastname: Ferreira-Fernandes
authors.affiliation: Departamento de Genética, Centro de Biociências, Universidade Federal de Pernambuco, Recife, PE, Brazil.
authors.forename: Carolina M M
authors.initials: CM
authors.lastname: Amaral
authors.affiliation: Faculdade de Medicina, Universidade Federal do Piauí, Teresina, PI, Brazil.
authors.forename: Lina G
authors.initials: LG
authors.lastname: Santos
authors.affiliation: Departamento de Genética, Centro de Biociências, Universidade Federal de Pernambuco, Recife, PE, Brazil.
authors.forename: Antônio C
authors.initials: AC
authors.lastname: Freitas
authors.affiliation: Departamento de Histologia e Embriologia, Centro de Biociências, Universidade Federal de Pernambuco, Recife, PE, Brazil.
authors.forename: Jacinto C
authors.initials: JC
authors.lastname: Silva-Neto
authors.affiliation: Molecular Oncogenetics Laboratory, Unidad de Investigación, Hospital Universitario La Paz, Madrid, Spain.
authors.forename: Juan A
authors.initials: JA
authors.lastname: Rey
authors.affiliation: Laboratório de Citogenética Humana, Instituto de Ciências Biológicas, Universidade Federal de Pará, Belém, PA, Brazil.
authors.forename: Rommel R
authors.initials: RR
authors.lastname: Burbano
authors.affiliation: Faculdade de Medicina, Universidade Federal do Piauí, Teresina, PI, Brazil.
authors.forename: Benedito B da
authors.initials: BB
authors.lastname: Silva
authors.affiliation: Laboratório de Genética e Biologia Molecular, Universidade Federal de Piauí, Parnaíba, PI, Brazil.
authors.forename: France K N
authors.initials: FK
authors.lastname: Yoshioka
authors.affiliation: Laboratório de Genética e Biologia Molecular, Universidade Federal de Piauí, Parnaíba, PI, Brazil.
authors.forename: Giovanny R
authors.initials: GR
authors.lastname: Pinto