title: Diosgenin effectively suppresses skin inflammation induced by phthalic anhydride in IL-4/Luc/CNS-1 transgenic mice.
abstract: To quantitatively evaluate the therapeutic effects of diosgenin (DG) and investigate the role of IL-4 on skin inflammation, alterations in luciferase-derived signal and general phenotype biomarkers were measured in IL-4/Luc/CNS-1 transgenic mice with phthalic anhydride (PA)-induced skin inflammation after treatment with DG for 4 weeks. High levels of luciferase-derived signal detected in the abdominal region and submandibular lymph node (SL) of the PA treated group was significantly decreased by 67-88% in the PA + DG cotreated group. Furthermore, the weight of the lymph node and spleen, IgE concentration, epidermis thickness, and number of infiltrated mast cells were lower in the PA + DG treated group than the PA + Vehicle treated group. Moreover, expression of IL-6 and vascular endothelial growth factor (VEGF) also decreased in the PA + DG cotreated group. These results suggest that PA-induced skin inflammation could be successfully suppressed by DG treatment in IL-4/Luc/CNS-1 Tg mice through attenuation of IL-4 and IL-6 expression, as well as decreased IgE concentration and mast cells infiltration.
authors.affiliation: a College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University , Miryang , Korea.
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Kim
authors.affiliation: a College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University , Miryang , Korea.
authors.forename: Jun
authors.initials: J
authors.lastname: Go
authors.affiliation: a College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University , Miryang , Korea.
authors.forename: Eun Kyoung
authors.initials: EK
authors.lastname: Koh
authors.affiliation: a College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University , Miryang , Korea.
authors.forename: Sung Hwa
authors.initials: SH
authors.lastname: Song
authors.affiliation: a College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University , Miryang , Korea.
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Sung
authors.affiliation: a College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University , Miryang , Korea.
authors.forename: Hyun Ah
authors.initials: HA
authors.lastname: Lee
authors.affiliation: a College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University , Miryang , Korea.
authors.forename: Dong Seob
authors.initials: DS
authors.lastname: Kim
authors.affiliation: a College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University , Miryang , Korea.
authors.forename: Hong Joo
authors.initials: HJ
authors.lastname: Son
authors.affiliation: b College of Human Ecology, Pusan National University , Busan , Korea.
authors.forename: Hee Seob
authors.initials: HS
authors.lastname: Lee
authors.affiliation: c Gangrim Organics , Miryang , Korea.
authors.forename: Chung Yeoul
authors.initials: CY
authors.lastname: Lee
authors.affiliation: d College of Pharmacy , Chungbuk National University , Chungju , Korea.
authors.forename: Jin Tae
authors.initials: JT
authors.lastname: Hong
authors.affiliation: a College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University , Miryang , Korea.
authors.forename: Dae Youn
authors.initials: DY
authors.lastname: Hwang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D003876:Dermatitis, Atopic / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D004144:Diosgenin / Q000494:pharmacology*; D015870:Gene Expression; D017930:Genes, Reporter; D007073:Immunoglobulin E / Q000235:genetics; D015847:Interleukin-4 / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000276:immunology; D015850:Interleukin-6 / Q000235:genetics* / Q000276:immunology; D008156:Luciferases / Q000235:genetics / Q000276:immunology; D008198:Lymph Nodes / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D051379:Mice; D008822:Mice, Transgenic; D009929:Organ Size / Q000187:drug effects; D010796:Phthalic Anhydrides; D012867:Skin / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D013154:Spleen / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000276:immunology; D000093242:Interleukin-6 Inhibitors
chemical_list: D000893:Anti-Inflammatory Agents; D004144:Diosgenin; D007073:Immunoglobulin E; D015847:Interleukin-4; D015850:Interleukin-6; D008156:Luciferases; D010796:Phthalic Anhydrides; D042461:Vascular Endothelial Growth Factor A; D000093242:Interleukin-6 Inhibitors; C577686:interleukin-6, mouse; C467480:vascular endothelial growth factor A, mouse; C043103:phthalic anhydride
keywords: IL-4/Luc/CNS-1 transgenic mice; IgE; diosgenin; phthalic anhydride; skin inflammation