title: Interferon-gamma release assays in patients with Mycobacterium kansasii pulmonary infection: A retrospective survey.
abstract: Interferon-gamma release assays (IGRAs) can be positive in patients infected with Mycobacterium kansasii (M. kansasii), which carries some of Mycobacterium tuberculosis specific antigens adopted for IGRAs. Our aim is to evaluate positive rate and factors associated with positive IGRAs in patients with M. kansasii pulmonary infection.
  
  
  
  We retrospectively investigated 105 M. kansasii cases in which IGRAs were performed before or ≦14 days after treatment initiation. Clinical characteristics including a history of tuberculosis, radiographic features and laboratory data were collected from medical records.
  
  
  
  Positive rate of each IGRA was 25.9% (15/58) in QuantiFERON TB-Gold (QFT-G), 31.8% (7/22) in QuantiFERON-TB Gold In Tube (QFT-GIT), and 33.3% (7/21) in T-SPOT. TB (T-SPOT). After excluding cases having a history of tuberculosis, positive rate of each IGRA decreased to 19% (8/42) in QFT-G, 20% (3/15) in QFT-GIT, and 18.8% (3/16) in T-SPOT. The multivariate analysis revealed that only previous tuberculosis was significantly associated with positive IGRAs (odds ratio, 4.758; 95% confidence interval, 1.73-13.05; p = 0.002).
  
  
  
  Positive rates of IGRAs were low in patients with M. kansasii, especially in those without previous tuberculosis. M. kansasii pulmonary infection alone might induce less interferon-gamma production with the antigens.
authors.affiliation: Center for Pulmonary Diseases, National Hospital Organization Tokyo National Hospital, 3-1-1 Takeoka, Kiyose-shi, Tokyo 204-8585, Japan. Electronic address: satour@tokyo-hosp.jp.
authors.forename: Ryota
authors.initials: R
authors.lastname: Sato
authors.affiliation: Center for Pulmonary Diseases, National Hospital Organization Tokyo National Hospital, 3-1-1 Takeoka, Kiyose-shi, Tokyo 204-8585, Japan. Electronic address: hnagai-in@tokyo-hosp.jp.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nagai
authors.affiliation: Center for Pulmonary Diseases, National Hospital Organization Tokyo National Hospital, 3-1-1 Takeoka, Kiyose-shi, Tokyo 204-8585, Japan. Electronic address: hmatui-in@tokyo-hosp.jp.
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Matsui
authors.affiliation: Center for Pulmonary Diseases, National Hospital Organization Tokyo National Hospital, 3-1-1 Takeoka, Kiyose-shi, Tokyo 204-8585, Japan. Electronic address: rs_98040@yahoo.co.jp.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Kawabe
authors.affiliation: Center for Pulmonary Diseases, National Hospital Organization Tokyo National Hospital, 3-1-1 Takeoka, Kiyose-shi, Tokyo 204-8585, Japan. Electronic address: takedak@tokyo-hosp.jp.
authors.forename: Keita
authors.initials: K
authors.lastname: Takeda
authors.affiliation: Center for Pulmonary Diseases, National Hospital Organization Tokyo National Hospital, 3-1-1 Takeoka, Kiyose-shi, Tokyo 204-8585, Japan. Electronic address: mkawashima-in@tokyo-hosp.jp.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kawashima
authors.affiliation: Center for Pulmonary Diseases, National Hospital Organization Tokyo National Hospital, 3-1-1 Takeoka, Kiyose-shi, Tokyo 204-8585, Japan. Electronic address: junkosz-in@tokyo-hosp.jp.
authors.forename: Junko
authors.initials: J
authors.lastname: Suzuki
authors.affiliation: Center for Pulmonary Diseases, National Hospital Organization Tokyo National Hospital, 3-1-1 Takeoka, Kiyose-shi, Tokyo 204-8585, Japan. Electronic address: ohshima-in@tokyo-hosp.jp.
authors.forename: Nobuharu
authors.initials: N
authors.lastname: Ohshima
authors.affiliation: Center for Pulmonary Diseases, National Hospital Organization Tokyo National Hospital, 3-1-1 Takeoka, Kiyose-shi, Tokyo 204-8585, Japan. Electronic address: kimihiko-in@tokyo-hosp.jp.
authors.forename: Kimihiko
authors.initials: K
authors.lastname: Masuda
authors.affiliation: Center for Pulmonary Diseases, National Hospital Organization Tokyo National Hospital, 3-1-1 Takeoka, Kiyose-shi, Tokyo 204-8585, Japan. Electronic address: yamanea@tokyo-hosp.jp.
authors.forename: Akira
authors.initials: A
authors.lastname: Yamane
authors.affiliation: Center for Pulmonary Diseases, National Hospital Organization Tokyo National Hospital, 3-1-1 Takeoka, Kiyose-shi, Tokyo 204-8585, Japan. Electronic address: tamura-in@tokyo-hosp.jp.
authors.forename: Atsuhisa
authors.initials: A
authors.lastname: Tamura
authors.affiliation: Center for Pulmonary Diseases, National Hospital Organization Tokyo National Hospital, 3-1-1 Takeoka, Kiyose-shi, Tokyo 204-8585, Japan. Electronic address: bob-in@tokyo-hosp.jp.
authors.forename: Shinobu
authors.initials: S
authors.lastname: Akagawa
authors.affiliation: Center for Pulmonary Diseases, National Hospital Organization Tokyo National Hospital, 3-1-1 Takeoka, Kiyose-shi, Tokyo 204-8585, Japan. Electronic address: kenohta@tokyo-hosp.jp.
authors.forename: Ken
authors.initials: K
authors.lastname: Ohta
mesh_terms: D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial / Q000276:immunology; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis* / Q000276:immunology; D059425:Interferon-gamma Release Tests; D055985:Latent Tuberculosis / Q000276:immunology / Q000382:microbiology; D008168:Lung / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D009165:Mycobacterium Infections, Nontuberculous / Q000276:immunology* / Q000382:microbiology; D019909:Mycobacterium kansasii / Q000276:immunology / Q000302:isolation & purification*; D009169:Mycobacterium tuberculosis / Q000276:immunology / Q000302:isolation & purification; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D011795:Surveys and Questionnaires; D014374:Tuberculin Test; D014376:Tuberculosis / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; C072681:Mycobacterium tuberculosis antigens; D007371:Interferon-gamma
keywords: Interferon-gamma release assays; Mycobacterium kansasii