title: Anticancer Activity Studies of Ruthenium(II) Complex Toward Human Osteosarcoma HOS Cells.
abstract: A new Ru(II) complex [Ru(dmp)2(NMIP)](ClO4)2 (dmp = 2,9-dimethyl-1,10-phenanthroline, NMIP = 2'-(2″-nitro-3″,4″-methylenedioxyphenyl)imidazo[4',5'-f][1,10]-phenanthroline) was synthesized and characterized by elemental analysis, ESI-MS and (1)H NMR. The cytotoxic activity of the complex against MG-63, U2OS, HOS, and MC3T3-e1 cell lines was investigated by MTT method. The complex shows moderate cytotoxicity toward HOS (IC50 = 35.6 ± 2.6 µM) and MC3T3-e1 (IC50 = 41.6 ± 2.8 µM) cell lines. The morphological studies show that the complex can induce apoptosis in HOS cells and cause an increase of reactive oxygen species levels and a decrease in the mitochondrial membrane potential. The cell cycle distribution demonstrates that the complex inhibits the cell growth at S phase. Additionally, the antitumor activity in vivo reveals that the complex can induce a decrease in tumor weight.
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510180, People's Republic of China.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Zhu
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510180, People's Republic of China.
authors.forename: Si-Hong
authors.initials: SH
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510180, People's Republic of China.
authors.forename: Gui-Qiang
authors.initials: GQ
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510180, People's Republic of China.
authors.forename: Hui-Hua
authors.initials: HH
authors.lastname: Xu
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510180, People's Republic of China.
authors.forename: Yu-Xuan
authors.initials: YX
authors.lastname: Wang
authors.affiliation: Department of Oncology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510180, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510180, People's Republic of China.
authors.forename: Ya-Min
authors.initials: YM
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510180, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510180, People's Republic of China.
authors.forename: Jun-Bo
authors.initials: JB
authors.lastname: Liang
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510180, People's Republic of China. qfguo@gzhmu.edu.cn.
authors.forename: Qi-Feng
authors.initials: QF
authors.lastname: Guo
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001859:Bone Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry / Q000494:pharmacology*; D012516:Osteosarcoma / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012428:Ruthenium / Q000737:chemistry; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D009942:Organometallic Compounds; D017382:Reactive Oxygen Species; D012428:Ruthenium
keywords: Apoptosis; Cell cycle arrest; Cytotoxicity in vitro and in vivo; Reactive oxygen species; Ruthenium(II) complex