title: Mathematics analysis of polymerase chain reaction kinetic curves.
abstract: The paper reviews different approaches to the mathematical analysis of polymerase chain reaction (PCR) kinetic curves. The basic principles of PCR mathematical analysis are presented. Approximation of PCR kinetic curves and PCR efficiency curves by various functions is described. Several PCR models based on chemical kinetics equations are suggested. Decision criteria for an optimal function to describe PCR efficiency are proposed.
authors.affiliation: JSC Syntol, ul. Timiryazevskaya 42, Moscow, 127550, Russia.
authors.forename: D G
authors.initials: DG
authors.lastname: Sochivko
authors.affiliation: Institute for Analytical Instrumentation, Russian Academy of Sciences, Rizhskii pr. 26, St. Petersburg, 190103, Russia. faa@bk.ru.
authors.forename: A A
authors.initials: AA
authors.lastname: Fedorov
authors.affiliation: All-Russia Research Institute of Agricultural Biotechnology, ul. Timiryazevskaya 42, Moscow, 127550, Russia.
authors.forename: D A
authors.initials: DA
authors.lastname: Varlamov
authors.affiliation: Institute for Analytical Instrumentation, Russian Academy of Sciences, Rizhskii pr. 26, St. Petersburg, 190103, Russia.
authors.forename: V E
authors.initials: VE
authors.lastname: Kurochkin
authors.affiliation: National Research Center, Institute of Immunology, Federal Biomedical Agency, Kashirskoe sh. 24, Moscow, 115478, Russia.
authors.forename: R V
authors.initials: RV
authors.lastname: Petrov
mesh_terms: D007700:Kinetics; D008962:Models, Theoretical; D016133:Polymerase Chain Reaction / Q000379:methods*