title: Time Dependent Distribution of MicroRNA 144 after Intravenous Delivery.
abstract: miR-144 has potential benefits in protecting against myocardial ischemia and suppression of tumor growth. We have previously shown that a single intravenous injection of miR-144 provides potent cardioprotection, but its kinetics and distribution are not known.
  
  
  
  Single stranded mature miR-144 or Cy3-labelled-miR-144 was delivered into C57/B6 mice by tail vein injection.
  
  
  
  After intravenous injection, the signal of Cy3-labelled-miR-144 in the kidney, brain, heart and liver peaks at 60 minutes, and is predominantly localised to the endothelium at that stage. In the kidney and heart, Cy3-labelled-miR-144 signal is detectable within the parenchymal tissues for at least 3 days, after which it starts to decrease, but brain Cy3-miR-144 signal rapidly decreases after 1 hour, and is lost at day 1, with no parenchymal uptake detected. Cy3-miR-144 signal can be detected until day 28 in the liver. Stem loop RTPCR confirmed the temporal pattern shown by miR-144 in kidney, brain and heart, but in liver there was a continuous rise following the initial injection until day 28 with no signs of decrease, suggesting de-novo synthesis.
  
  
  
  There is early endothelial uptake of injected miR-144 followed by organ-specific distribution and kinetics. In the liver, there appears to be a positive feedback process that leads to continued accumulation of miR-144 that persists for at least 28 days. These observations should be taken into account when designing experiments utilizing parenteral miR-144 and assessing the biology of its actions.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Sean
authors.initials: S
authors.lastname: Cai
authors.forename: Jenny
authors.initials: J
authors.lastname: Peng
authors.forename: Mark K
authors.initials: MK
authors.lastname: Friedberg
authors.affiliation: Heart Institute, UC Department of Pediatrics3333 Burnet Avenue Cincinnati Ohio 45229-3026, USA.
authors.forename: Andrew N
authors.initials: AN
authors.lastname: Redington
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D060565:Argonaute Proteins / Q000378:metabolism; D001921:Brain / Q000166:cytology*; D002232:Carbocyanines; D007668:Kidney / Q000166:cytology*; D008099:Liver / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D009206:Myocardium / Q000166:cytology*; D013194:Staining and Labeling
chemical_list: C526735:Ago2 protein, mouse; D060565:Argonaute Proteins; D002232:Carbocyanines; C551064:MIRN144 microRNA, mouse; D035683:MicroRNAs; C089187:cyanine dye 3