title: Modulating intracellular acidification by regulating the incubation time of proton caged compounds.
abstract: A proton caged compound, the 1-(2-nitrophenyl)- ethylhexadecyl sulfonate (HDNS), was dosed into HEK-293 at different incubation times. Samples were irradiated with filtered UV light for inducing photolysis of the HDNS and then probed by infrared spectroscopy. The intracellular acidification reaction can be followed by monitoring the consequent CO2 peak intensity variation. The total CO2 produced is similar for all the samples, hence it is only a function of the initial HDNS concentration. The way it is achieved, though, is different for the different incubation times and follows kinetics, which results in a combination of a linear CO2 increase and a steep CO2 increase followed by a decay. This is interpreted in terms of confinement of the HDNS into intracellular vesicles of variable average size and sensitive to UV light when they reach critical dimensions.
authors.affiliation: Department of Chemical Sciences and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica, 1, 00133, Rome, Italy. marilena.carbone@roma2.infn.it.|Consorzio Interuniversitario Biostrutture e Biosistemi, Viale Medaglie d'Oro 305, 00136, Rome, Italy. marilena.carbone@roma2.infn.it.
authors.forename: Marilena
authors.identifier: http://orcid.org/0000-0002-4224-1393
authors.initials: M
authors.lastname: Carbone
authors.affiliation: Department of Chemical Sciences and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica, 1, 00133, Rome, Italy.|Department of Chemistry, University of Rome La Sapienza, P.le A. Moro, 00185, Rome, Italy.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Sabbatella
authors.affiliation: Department of Chemical Sciences and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica, 1, 00133, Rome, Italy.
authors.forename: Simonetta
authors.initials: S
authors.lastname: Antonaroli
authors.affiliation: Department of Biology and Biotechnology "Charles Darwin", University of Rome La Sapienza, P.le A. Moro, 00185, Rome, Italy.
authors.forename: Viviana
authors.initials: V
authors.lastname: Orlando
authors.affiliation: Department of Biology and Biotechnology "Charles Darwin", University of Rome La Sapienza, P.le A. Moro, 00185, Rome, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Biagioni
authors.affiliation: Department of Physics, University of Rome La Sapienza, P.le A. Moro, 00185, Rome, Italy.|Center for Life Nano Science, Istituto Italiano di Tecnologia, Viale Regina Elena 291, 00161, Rome, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Nucara
mesh_terms: D002245:Carbon Dioxide / Q000737:chemistry / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D042541:Intracellular Space / Q000737:chemistry* / Q000187:drug effects* / Q000378:metabolism; D011522:Protons; D013451:Sulfonic Acids / Q000494:pharmacology*
chemical_list: C000614029:1-(2-nitrophenyl)-ethylhexadecylsulfonate; D011522:Protons; D013451:Sulfonic Acids; D002245:Carbon Dioxide
keywords: CO2 monitoring; Intracellular acidification; Proton caged compounds