title: Dietary Lipid and Carbohydrate Interactions: Implications on Lipid and Glucose Absorption, Transport in Gilthead Sea Bream (Sparus aurata) Juveniles.
abstract: A digestibility trial was performed with gilthead sea bream juveniles (IBW = 72 g) fed four diets differing in lipid source (fish oil, FO; or a blend of vegetable oil, VO) and starch content (0 %, CH-; or 20 %, CH+) to evaluate the potential interactive effects between carbohydrates and VO on the processes involved in digestion, absorption and transport of lipids and glucose. In fish fed VO diets a decrease in lipid digestibility and in cholesterol (C), High Density Lipoprotein(HDL)-C and Low Density Lipoprotein (LDL)-C (only in CH+ group) were recorded. Contrarily, dietary starch induced postprandial hyperglycemia and time related alterations on serum triacylglycerol (TAG), phospholipid (PL) and C concentrations. Fish fed a CH+ diet presented lower serum TAG than CH- group at 6 h post-feeding, and the reverse was observed at 12 h post-feeding for TAG and PL. Lower serum C and PL at 6 h post-feeding were recorded only in VOCH+ group. No differences between groups were observed in hepatic and intestinal transcript levels of proteins involved in lipid transport and hydrolysis (FABP, DGAT, GPAT, MTP, LPL, LCAT). Lower transcript levels of proteins related to lipid transport (ApoB, ApoA1, FABP2) were observed in the intestine of fish fed the CH+ diet, but remained unchanged in the liver. Overall, transcriptional mechanisms involved in lipid transport and absorption were not linked to changes in lipid serum and digestibility. Dietary starch affected lipid absorption and transport, probably due to a delay in lipid absorption. This study suggests that a combination of dietary VO and starch may negatively affect cholesterol absorption and transport.
authors.affiliation: Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, Edifício FC4, 4169-007, Porto, Portugal. carolinacastro23@gmail.com.|CIMAR/CIIMAR-Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Rua dos Bragas 289, 4050-123, Porto, Portugal. carolinacastro23@gmail.com.
authors.forename: Carolina
authors.initials: C
authors.lastname: Castro
authors.affiliation: INRA, UR1067 Nutrition Metabolism Aquaculture, 64310, Saint-Pée-Sur-Nivelle, France.
authors.forename: Geneviève
authors.initials: G
authors.lastname: Corraze
authors.affiliation: Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, Edifício FC4, 4169-007, Porto, Portugal.
authors.forename: Ana
authors.initials: A
authors.lastname: Basto
authors.affiliation: INRA, UR1067 Nutrition Metabolism Aquaculture, 64310, Saint-Pée-Sur-Nivelle, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Larroquet
authors.affiliation: INRA, UR1067 Nutrition Metabolism Aquaculture, 64310, Saint-Pée-Sur-Nivelle, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Panserat
authors.affiliation: Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, Edifício FC4, 4169-007, Porto, Portugal.|CIMAR/CIIMAR-Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Rua dos Bragas 289, 4050-123, Porto, Portugal.
authors.forename: Aires
authors.initials: A
authors.lastname: Oliva-Teles
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D008076:Cholesterol, HDL / Q000378:metabolism; D008078:Cholesterol, LDL / Q000378:metabolism; D004040:Dietary Carbohydrates / Q000008:administration & dosage* / Q000009:adverse effects; D004041:Dietary Fats / Q000008:administration & dosage / Q000009:adverse effects; D004063:Digestion / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D005819:Genetic Markers / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism*; D007408:Intestinal Absorption / Q000187:drug effects; D050356:Lipid Metabolism / Q000187:drug effects; D010938:Plant Oils / Q000008:administration & dosage* / Q000009:adverse effects; D021541:Sea Bream / Q000502:physiology*; D013213:Starch / Q000009:adverse effects
chemical_list: D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D005819:Genetic Markers; D010938:Plant Oils; D013213:Starch; D002784:Cholesterol; D005947:Glucose
keywords: Alternative ingredients; Digestibility; Gene expression; Nutrient absorption and transport; Serum metabolites