title: Rpb1 foot mutations demonstrate a major role of Rpb4 in mRNA stability during stress situations in yeast.
abstract: The RPB1 mutants in the foot region of RNA polymerase II affect the assembly of the complex by altering the correct association of both the Rpb6 and the Rpb4/7 dimer. Assembly defects alter both transcriptional activity as well as the amount of enzyme associated with genes. Here, we show that the global transcriptional analysis of foot mutants reveals the activation of an environmental stress response (ESR), which occurs at a permissive temperature under optimal growth conditions. Our data indicate that the ESR that occurs in foot mutants depends mostly on a global post-transcriptional regulation mechanism which, in turn, depends on Rpb4-mRNA imprinting. Under optimal growth conditions, we propose that Rpb4 serves as a key to globally modulate mRNA stability as well as to coordinate transcription and decay. Overall, our results imply that post-transcriptional regulation plays a major role in controlling the ESR at both the transcription and mRNA decay levels.
authors.affiliation: Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Paraje de las Lagunillas, s/n, 23071 Jaén, Spain.
authors.forename: A I
authors.initials: AI
authors.lastname: Garrido-Godino
authors.affiliation: Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Paraje de las Lagunillas, s/n, 23071 Jaén, Spain.
authors.forename: M C
authors.initials: MC
authors.lastname: García-López
authors.affiliation: Departamento de Genética, Facultad de Biológicas, Universitat de València, Dr Moliner 50, E-46100 Burjassot, Valencia, Spain; ERI Biotecmed, Universitat de València, Dr Moliner 50, E-46100 Burjassot, Valencia, Spain.
authors.forename: J
authors.initials: J
authors.lastname: García-Martínez
authors.affiliation: European Molecular Biology Laboratories (EMBL), Genome Biology Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Pelechano
authors.affiliation: ERI Biotecmed, Universitat de València, Dr Moliner 50, E-46100 Burjassot, Valencia, Spain; Departamento de Bioquímica y Biología Molecular, Facultad de Biológicas, Universitat de València, Dr Moliner 50, E-46100 Burjassot, Valencia, Spain.
authors.forename: D A
authors.initials: DA
authors.lastname: Medina
authors.affiliation: ERI Biotecmed, Universitat de València, Dr Moliner 50, E-46100 Burjassot, Valencia, Spain; Departamento de Bioquímica y Biología Molecular, Facultad de Biológicas, Universitat de València, Dr Moliner 50, E-46100 Burjassot, Valencia, Spain. Electronic address: jose.e.perez@uv.es.
authors.forename: J E
authors.initials: JE
authors.lastname: Pérez-Ortín
authors.affiliation: Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Paraje de las Lagunillas, s/n, 23071 Jaén, Spain. Electronic address: fngomez@ujaen.es.
authors.forename: F
authors.initials: F
authors.lastname: Navarro
mesh_terms: D054802:Molecular Imprinting; D009154:Mutation; D012319:RNA Polymerase II / Q000235:genetics*; D020871:RNA Stability / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D013312:Stress, Physiological / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D012319:RNA Polymerase II; C511736:RPB1 protein, S cerevisiae; C423309:RPB4 protein, S cerevisiae
keywords: Assembly; Rpb4; Stability; Stress; mRNA