title: Hydrogen Attachment/Abstraction Dissociation (HAD) of Gas-Phase Peptide Ions for Tandem Mass Spectrometry.
abstract: Dissociation of gas-phase peptide ions through interaction with low-energy hydrogen (H) radical (∼0.15 eV) was observed with a quadrupole ion trap mass spectrometry. The H radical generated by thermal dissociation of H2 molecules passing through a heated tungsten capillary (∼2000 °C) was injected into the ion trap containing target peptide ions. The fragmentation spectrum showed abundant c-/z- and a-/x-type ions, attributable to H attachment/abstraction to/from peptide ion. Because the low-energy neutral H radical initiated the fragmentation, the charge state of the precursor ion was maintained during the dissociation. As a result, precursor ions of any charge state, including singly charged positive and negative ions, could be analyzed for amino acid sequence. The sequence coverage exceeding 90% was obtained for both singly protonated and singly deprotonated substance P peptide. This mass spectrometry also preserved labile post-translational modification bonds. The modification sites of triply phosphorylated peptide (kinase domain of insulin receptor) were identified with the sequence coverage exceeding 80%.
authors.affiliation: Koichi Tanaka Mass Spectrometry Research Laboratory, Shimadzu Corporation , 1 Nishinokyo-Kuwabaracho Nakagyo-ku, Kyoto 604-8511, Japan.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: Koichi Tanaka Mass Spectrometry Research Laboratory, Shimadzu Corporation , 1 Nishinokyo-Kuwabaracho Nakagyo-ku, Kyoto 604-8511, Japan.
authors.forename: Sadanori
authors.initials: S
authors.lastname: Sekiya
authors.affiliation: Koichi Tanaka Mass Spectrometry Research Laboratory, Shimadzu Corporation , 1 Nishinokyo-Kuwabaracho Nakagyo-ku, Kyoto 604-8511, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Nishikaze
authors.affiliation: Koichi Tanaka Mass Spectrometry Research Laboratory, Shimadzu Corporation , 1 Nishinokyo-Kuwabaracho Nakagyo-ku, Kyoto 604-8511, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Kodera
authors.affiliation: Koichi Tanaka Mass Spectrometry Research Laboratory, Shimadzu Corporation , 1 Nishinokyo-Kuwabaracho Nakagyo-ku, Kyoto 604-8511, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Iwamoto
authors.affiliation: Graduate School of Science and Engineering, Doshisha University , Kyotanabe, Kyoto 610-0321, Japan.
authors.forename: Motoi
authors.initials: M
authors.lastname: Wada
authors.affiliation: Koichi Tanaka Mass Spectrometry Research Laboratory, Shimadzu Corporation , 1 Nishinokyo-Kuwabaracho Nakagyo-ku, Kyoto 604-8511, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Tanaka
mesh_terms: D000595:Amino Acid Sequence; D006859:Hydrogen / Q000737:chemistry*; D010455:Peptides / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010455:Peptides; D006859:Hydrogen