title: The Effect of rhCygb on CCl4-Induced Hepatic Fibrogenesis in Rat.
abstract: This study aims to investigate whether the use of recombinant human cytoglobin (rhCygb) impact on hepatic fibrogenesis caused by CCl4. SD (n = 150) rats were randomly divided into three groups of normal, CCl4 model and rhCygb groups. After model establishment, rats in rhCygb groups were administered daily with rhCygb (2 mg/kg, s.c.). Histological lesions were staged according to metavir. Serum parameters including ALT, AST, HA, LN, Col III and Col IV were determined. The liver proteins were separated by 2-DE and identified. As a result, the stage of hepatic damage and liver fibrosis in rhCygb groups were significantly milder than that in CCl4 model groups. Meanwhile, rhCygb dramatically reversed serum levels of ALT and AST, and also markedly decreased the liver fibrosis markers levels of LN, HA, Col III and Col IV. In 2-DE, 33 proteins among three groups with the same changing tendency in normal and rhCygb treated groups compared with CCl4 model group were identified. GO analysis showed that several identified proteins involved in oxidative stress pathway. The study provides new insights and data for administration of rhCygb reversing CCl4-induced liver fibrosis suggesting that rhCygb might be used in the treatment of liver fibrosis.
authors.affiliation: School of Biotechnology, Southern Medical University, Guangzhou, Guangdong Province 510515, P.R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.affiliation: School of Biotechnology, Southern Medical University, Guangzhou, Guangdong Province 510515, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: School of Biotechnology, Southern Medical University, Guangzhou, Guangdong Province 510515, P.R. China.
authors.forename: Bohong
authors.initials: B
authors.lastname: Chen
authors.affiliation: School of Biotechnology, Southern Medical University, Guangzhou, Guangdong Province 510515, P.R. China.
authors.forename: Gaotai
authors.initials: G
authors.lastname: Cai
authors.affiliation: School of Biotechnology, Southern Medical University, Guangzhou, Guangdong Province 510515, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Biotechnology, Southern Medical University, Guangzhou, Guangdong Province 510515, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: School of Biotechnology, Southern Medical University, Guangzhou, Guangdong Province 510515, P.R. China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Tang
authors.affiliation: School of Biotechnology, Southern Medical University, Guangzhou, Guangdong Province 510515, P.R. China.
authors.forename: Wenqi
authors.initials: W
authors.lastname: Dong
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D015415:Biomarkers / Q000097:blood*; D002251:Carbon Tetrachloride / Q000009:adverse effects*; D000078842:Cytoglobin; D004195:Disease Models, Animal; D004334:Drug Administration Schedule; D005786:Gene Expression Regulation / Q000187:drug effects; D005914:Globins / Q000008:administration & dosage* / Q000494:pharmacology; D006801:Humans; D008099:Liver / Q000378:metabolism; D008103:Liver Cirrhosis / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy*; D018384:Oxidative Stress / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015415:Biomarkers; C000627265:CYGB protein, human; C000627268:Cygb protein, rat; D000078842:Cytoglobin; D005914:Globins; D002251:Carbon Tetrachloride; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase