title: Comparative polyphenolic content and antioxidant activities of Genista tinctoria L. and Genistella sagittalis (L.) Gams (Fabaceae).
abstract: The aim of this study was focused on the polyphenolic composition and antioxidant capacity of Genista tinctoria L. and Genistella sagittalis (L.) Gams. A qualitative and quantitative characterization of the main phenolic compounds from the extracts were carried out using a HPLC-MS method. The total polyphenolic and flavonoid content was spectrophotometrically determined. The antioxidant activity towards various radicals generated in different systems was evaluated usingDPPH bleaching method, Trolox equivalent antioxidant capacity assay (TEAC) and Oxygen radical absorbance capacity (ORAC), and all indicated that G. tinctoria extract was more antioxidant than G. sagittalis extract.That was in good agreement with the total polyphenolic and flavonoidic content.Chlorogenic acid, p-coumaric acid, isoquercitrin and apigenin were identified in bothspecies. Caffeic acid, ferulic acid, hyperoside, rutin, quercitrin and luteolin were found only in G. tinctoria, while quercetin was determined in G. sagittalis.
authors.affiliation: Department of Pharmacognosy "Iuliu Hatieganu" University of Medicine and Pharmacy, Creanga Street, Cluj-Napoca, Romania.
authors.forename: Daniela
authors.initials: D
authors.lastname: Hanganu
authors.affiliation: Department of Therapeutical Chemistry and Pharmaceutical Industry, Faculty of Pharmacy, Vasile Goldis West University of Arad, 86 L. Rebreanu Street, Arad, Romania and SC PlantExtrakt SRL, Radaia, Jud. Cluj, Romania.
authors.forename: Neli Kinga
authors.initials: NK
authors.lastname: Olah
authors.affiliation: Department of Pharmacognosy "Iuliu Hatieganu" University of Medicine and Pharmacy, Creanga Street, Cluj-Napoca, Romania.
authors.forename: Daniela
authors.initials: D
authors.lastname: Benedec
authors.affiliation: Department of Pharmaceutical Botany, Faculty of Pharmacy, "Iuliu Hatieganu" University of Medicine and Pharmacy, Creanga Street, Cluj-Napoca, Romania.
authors.forename: Andrei
authors.initials: A
authors.lastname: Mocan
authors.affiliation: Department of Pharmaceutical Botany, Faculty of Pharmacy, "Iuliu Hatieganu" University of Medicine and Pharmacy, Creanga Street, Cluj-Napoca, Romania.
authors.forename: Gianina
authors.initials: G
authors.lastname: Crisan
authors.affiliation: Department of Pharmaceutical Technology and Biopharmaceutics, Creanga Street, Cluj-Napoca, Romania.
authors.forename: Laurian
authors.initials: L
authors.lastname: Vlase
authors.affiliation: Department of Pharmacognosy "Iuliu Hatieganu" University of Medicine and Pharmacy, Creanga Street, Cluj-Napoca, Romania.
authors.forename: Iulia
authors.initials: I
authors.lastname: Popica
authors.affiliation: Department of Pharmacognosy "Iuliu Hatieganu" University of Medicine and Pharmacy, Creanga Street, Cluj-Napoca, Romania.
authors.forename: Ilioara
authors.initials: I
authors.lastname: Oniga
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D002839:Chromans / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D007887:Fabaceae / Q000737:chemistry*; D047309:Flavones / Q000737:chemistry / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism; D039761:Genista / Q000737:chemistry*; D013058:Mass Spectrometry; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D059808:Polyphenols / Q000737:chemistry* / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000737:chemistry
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D002839:Chromans; D047309:Flavones; D016166:Free Radical Scavengers; D005609:Free Radicals; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; D017382:Reactive Oxygen Species; C004931:1,1-diphenyl-2-picrylhydrazyl; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid