title: Association of the osteopontin rs1126616 polymorphism and a higher serum osteopontin level with lupus nephritis.
abstract: Osteopontin (OPN) is a chemokine-like glycoprotein that has a prominent role in regulating inflammation and immunity.OPNpolymorphisms and elevated OPN levels are associated with systemic lupus erythematosus (SLE) in several populations. The aim of present study was to evaluate the association between theOPNrs1126616 polymorphism and OPN level with SLE susceptibility. A total of 163 SLE patients and 180 age-, gender- and ethnically matched controls were genotyped for the rs1126616 polymorphism by the polymerase chain reaction-restriction fragment length polymorphism method. Serum OPN levels were assayed by the enzyme-linked immunosorbent assay. There was no association between theOPNrs1126616 C/T polymorphism and SLE. The frequency of theOPNrs1126616 CT genotype was significantly higher in SLE patients with nephritis compared to SLE patients without nephritis and controls. Additionally, the frequency of TT genotypes was higher in SLE patients with nephritis compared to controls. The serum OPN levels were significantly higher in SLE patients compared to controls (50.6±22 vs. 35.6±15.8 ng/ml, P<0.001). Increased serum OPN levels were observed in SLE patients with lupus nephritis and joint symptoms. There was no correlation between OPN levels and theOPNrs1126616 polymorphism. The present data suggest that the CT and TT genotypes of theOPNrs1126616 polymorphism could be a risk factor for lupus nephritis. The OPN level is associated with SLE and certain SLE manifestations. However, there was no association between theOPNrs1126616 C/T polymorphism and SLE susceptibility.
authors.affiliation: Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan 9816743175, Iran; Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan 9816743175, Iran.
authors.forename: Saeedeh
authors.initials: S
authors.lastname: Salimi
authors.affiliation: Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan 9816743175, Iran.
authors.forename: Mehrangiz
authors.initials: M
authors.lastname: Noora
authors.affiliation: Department of English Language, School of Medicine, Zahedan University of Medical Sciences, Zahedan 9816743175, Iran.
authors.forename: Sima
authors.initials: S
authors.lastname: Nabizadeh
authors.affiliation: Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan 9816743175, Iran.
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Rezaei
authors.affiliation: Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan 9816743175, Iran.
authors.forename: Hossain
authors.initials: H
authors.lastname: Shahraki
authors.affiliation: Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan 9816743175, Iran; Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan 9816743175, Iran.
authors.forename: Mohammadoo-Khorassani
authors.initials: MK
authors.lastname: Milad
authors.affiliation: Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan 9816743175, Iran.
authors.forename: Anoosh
authors.initials: A
authors.lastname: Naghavi
authors.affiliation: Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan 9816743175, Iran; Department of Pharmacology, School of Medicine, Zahedan University of Medical Sciences, Zahedan 9816743175, Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Farajian-Mashhadi
authors.affiliation: Department of Internal Medicine, School of Medicine, Zahedan University of Medical Sciences, Zahedan 9816743175, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Zakeri
authors.affiliation: Department of Internal Medicine, School of Medicine, Zahedan University of Medical Sciences, Zahedan 9816743175, Iran.
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Sandoughi
keywords: gene; lupus nephritis; osteopontin; polymorphism; systemic lupus erythematosus