title: Large Porous Particles for Sustained Release of a Decoy Oligonucelotide and Poly(ethylenimine): Potential for Combined Therapy of Chronic Pseudomonas aeruginosa Lung Infections.
abstract: We have recently demonstrated that the specific inhibition of nuclear factor-κB by a decoy oligonucleotide (dec-ODN) delivered through inhalable large porous particles (LPP) made of poly(lactic-co-glycolic acid) (PLGA) may be highly beneficial for long-term treatment of lung inflammation. Nevertheless, besides chronic inflammation, multifunctional systems aimed to control also infection are required in chronic lung diseases, such as cystic fibrosis (CF). In this work, we tested the hypothesis that engineering PLGA-based LPP with branched poly(ethylenimine) (PEI) may improve LPP properties for pulmonary delivery of dec-ODN, with particular regard to the treatment of Pseudomonas aeruginosa lung infections. After getting insight into the role of PEI on the technological properties of PLGA-based LPP for delivery of dec-ODN, the putative synergistic effect of PEI free or PEI released from LPP on in vitro antimicrobial activity of tobramycin (Tb) and aztreonam (AZT) against P. aeruginosa was elucidated. Meanwhile, cytotoxicity studies on A549 cells were carried out. Results clearly demonstrate that the dry powders have promising aerosolization properties and afford a prolonged in vitro release of both dec-ODN and PEI. The encapsulation of PEI into LPP results in a 2-fold reduction of the minimum inhibitory concentration of AZT, while reducing the cytotoxic effect of PEI. Of note, the developed ODN/PLGA/PEI LPP persisted at lung at least for 14 days after intratracheal administration in rats where they can provide sustained and combined release of dec-ODN and PEI. dec-ODN will likely act as an anti-inflammatory drug, while PEI may enhance the therapeutic activity of inhaled antibiotics, which are commonly employed for the treatment of concomitant lung infections.
authors.affiliation: Di.S.T.A.Bi.F., Second University of Naples , Via Vivaldi 43, 81100 Caserta, Italy.
authors.forename: Ivana
authors.initials: I
authors.lastname: d'Angelo
authors.affiliation: Department of Experimental Medicine- Section of Microbiology, Second University of Naples , Via Santa Maria di Costantinopoli 16, 80138 Naples, Italy.
authors.forename: Brunella
authors.initials: B
authors.lastname: Perfetto
authors.affiliation: Drug Delivery Laboratories, Department of Pharmacy, University of Naples Federico II , Via Domenico Montesano 49, 80131 Naples, Italy.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Costabile
authors.affiliation: Department of Experimental Medicine- Section of Microbiology, Second University of Naples , Via Santa Maria di Costantinopoli 16, 80138 Naples, Italy.
authors.forename: Veronica
authors.initials: V
authors.lastname: Ambrosini
authors.affiliation: Department of Experimental Medicine- Section of Microbiology, Second University of Naples , Via Santa Maria di Costantinopoli 16, 80138 Naples, Italy.
authors.forename: Pina
authors.initials: P
authors.lastname: Caputo
authors.affiliation: Drug Delivery Laboratories, Department of Pharmacy, University of Naples Federico II , Via Domenico Montesano 49, 80131 Naples, Italy.
authors.forename: Agnese
authors.initials: A
authors.lastname: Miro
authors.affiliation: Pharmacology Laboratories, Department of Pharmacy, University of Naples Federico II , Via Domenico Montesano 49, 80131 Naples, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: d'Emmanuele di Villa Bianca
authors.affiliation: Pharmacology Laboratories, Department of Pharmacy, University of Naples Federico II , Via Domenico Montesano 49, 80131 Naples, Italy.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Sorrentino
authors.affiliation: Department of Experimental Medicine- Section of Microbiology, Second University of Naples , Via Santa Maria di Costantinopoli 16, 80138 Naples, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Donnarumma
authors.affiliation: Drug Delivery Laboratories, Department of Pharmacy, University of Naples Federico II , Via Domenico Montesano 49, 80131 Naples, Italy.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Quaglia
authors.affiliation: Drug Delivery Laboratories, Department of Pharmacy, University of Naples Federico II , Via Domenico Montesano 49, 80131 Naples, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Ungaro
mesh_terms: D000818:Animals; D002908:Chronic Disease; D004337:Drug Carriers / Q000737:chemistry*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D008297:Male; D009841:Oligonucleotides / Q000008:administration & dosage* / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016062:Porosity; D011552:Pseudomonas Infections / Q000188:drug therapy* / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012141:Respiratory Tract Infections / Q000188:drug therapy* / Q000382:microbiology
chemical_list: D004337:Drug Carriers; D009841:Oligonucleotides; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011094:Polyethyleneimine