title: A proteomic analysis of mushroom polysaccharide-treated HepG2 cells.
abstract: The anti-tumor properties of fungal polysaccharides have gained significant recognition in Asia and tropical America. In this study, the differential expression of proteins in normal HepG2 cells and those treated with polysaccharides that had been isolated from Phellinus linteus (PL), Ganoderma lucidum (GL) and Auricularia auricula (AA) was investigated. Using two-dimensional electrophoresis (2DE), a total of 104 protein spots were determined to be overexpressed in these cells compared with noncancerous regions. A total of 59 differentially expressed proteins were identified through MALDI-TOF-MS. In addition, 400 biological processes (BP), 133 cell components (CC) and 146 molecular functions (MF) were enriched by Gene Ontology (GO) analysis, and 78 KEGG pathways were enriched by pathway enrichment. Protein-Protein Interaction (PPI) analysis demonstrated the interaction networks affected by polysaccharides in HepG2 cells. Then, DJ-1 and 14-3-3 were identified as the key proteins in the networks, and the expression of the mRNA and proteins were evaluated using Real-time quantitative PCR (qRT-PCR) and Western blotting (WB), respectively. The results were in agreement with the 2DE. These results provided information on significant proteins of hepatocellular carcinoma (HCC) and form an important basis for the future development of valuable medicinal mushroom resources.
authors.affiliation: College of Life Sciences, Northeast Forestry University, Harbin, PR China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Chai
authors.affiliation: College of Life Sciences, Northeast Forestry University, Harbin, PR China.
authors.forename: Guibin
authors.initials: G
authors.lastname: Wang
authors.affiliation: Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences,Harbin, PR China.
authors.forename: Lili
authors.initials: L
authors.lastname: Fan
authors.affiliation: College of Life Sciences, Northeast Forestry University, Harbin, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhao
mesh_terms: D048948:14-3-3 Proteins / Q000235:genetics / Q000378:metabolism; D000363:Agaricales / Q000737:chemistry*; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D063990:Gene Ontology; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D011134:Polysaccharides / Q000494:pharmacology*; D000071617:Protein Deglycase DJ-1 / Q000235:genetics / Q000378:metabolism; D060066:Protein Interaction Maps / Q000187:drug effects / Q000235:genetics; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D048948:14-3-3 Proteins; D011134:Polysaccharides; D020543:Proteome; D000071617:Protein Deglycase DJ-1