title: Development and characterization of surface engineered PPI dendrimers for targeted drug delivery.
abstract: In this study, we reported folate-conjugated polypropylene imine dendrimers (FA-PPI) as efficient carrier for model anticancer drug, methotrexate (MTX), for pH-sensitive drug release, selective targeting to cancer cells, and anticancer activity. In the in vitro drug release studies this nanoconjugate of MTX showed initial rapid release followed by gradual slow release, and the drug release was found to be pH sensitive with greater release at acidic pH. The ex vivo investigations with human breast cancer cell lines, MCF-7, showed enhanced cytotoxicity of MTX-FA-PPI with significantly enhanced intracellular uptake. The biofate of nanoconjugate was determined in Wistar rat where MTX-FA-PPI showed 37.79-fold increase in the concentration of MTX in liver after 24 h in comparison with free MTX formulation.
authors.affiliation: a Pharmaceutical Nanotechnology Research Laboratory, ISF College of Pharmacy , Moga , Punjab , India.
authors.forename: Avleen
authors.initials: A
authors.lastname: Kaur
authors.affiliation: a Pharmaceutical Nanotechnology Research Laboratory, ISF College of Pharmacy , Moga , Punjab , India.|b National Institute of Pharmaceutical Education and Research (NIPER Raebareli) , Raebareli , Uttar Pradesh , India.
authors.forename: Keerti
authors.initials: K
authors.lastname: Jain
authors.affiliation: a Pharmaceutical Nanotechnology Research Laboratory, ISF College of Pharmacy , Moga , Punjab , India.|c Department of Pharmaceutical Sciences, Irma Lerma Rangel College of Pharmacy , Texas A & M Health Science Center, Texas A & M University , Kingsville , TX , USA.
authors.forename: Neelesh Kumar
authors.initials: NK
authors.lastname: Mehra
authors.affiliation: a Pharmaceutical Nanotechnology Research Laboratory, ISF College of Pharmacy , Moga , Punjab , India.|d School of Pharmaceutical Sciences, Rajiv Gandhi Technical University , Bhopal , Madhya Pradesh , India.
authors.forename: N K
authors.initials: NK
authors.lastname: Jain
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000097:blood / Q000493:pharmacokinetics* / Q000494:pharmacology; D001682:Biological Availability; D001692:Biological Transport; D002470:Cell Survival / Q000187:drug effects; D050091:Dendrimers / Q000737:chemistry* / Q000378:metabolism; D004337:Drug Carriers; D004339:Drug Compounding; D065546:Drug Liberation; D005260:Female; D005492:Folic Acid / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D061986:MCF-7 Cells; D008727:Methotrexate / Q000097:blood / Q000493:pharmacokinetics* / Q000494:pharmacology; D058990:Molecular Targeted Therapy; D009928:Organ Specificity; D011126:Polypropylenes / Q000737:chemistry* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000970:Antineoplastic Agents; D050091:Dendrimers; D004337:Drug Carriers; D011126:Polypropylenes; C443641:poly(propyleneimine); D005492:Folic Acid; D008727:Methotrexate
keywords: Cancer; dendrimers; drug targeting; folic acid; methotrexate