title: Erectogenic and Aphrodisiac Property of Moringa oleifera: Involvement of Soluble Epoxide Hydrolase Enzyme.
abstract: Soluble epoxide hydrolase (sEH) inhibitors have been reported to improve penile erection; therefore, sEH could be useful for management of erectile dysfunction. Methanolic and aqueous extracts of 30 Indian medicinal plants were screened for their sEH inhibition potential. Fifteen extracts showed >50% inhibition when screened at 50 µg/mL in sEH inhibition assay. Methanolic extract of Moringa oleifera Lam. (Moringaceae) seeds (MEMO) was most potent with IC50 1.7 ± 0.1 µg/mL and was selected for in vitro studies on isolated rat corpus cavernosum smooth muscle and in vivo sexual behaviour studies on healthy and diabetic rats. Rats were divided into five groups, each containing six animals and treated orally with either water, vehicle (1% Tween-20), MEMO (45 and 90 mg/kg/day for 21 days), and standard drug, sildenafil (5 mg/kg/day for 7 days). An equal number of female rats were used, and the effect of MEMO and sildenafil was compared with that of vehicle. MEMO significantly relaxed isolated rat corpus cavernosum smooth muscle at 0.1-100 µg/mL in vitro and significantly increased (p < 0.05) sexual activity, intracavernous pressure/mean arterial pressure in normal and diabetic rats. The increase in erectile function of rats by MEMO could be because of its sEH inhibitory activity. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Pharmacology, Al-Ameen College of Pharmacy, Bangalore, 560027, India.
authors.forename: Sumanta Kumar
authors.initials: SK
authors.lastname: Goswami
authors.affiliation: Department of Pharmacology, Al-Ameen College of Pharmacy, Bangalore, 560027, India.
authors.forename: Mohammed Naseeruddin
authors.initials: MN
authors.lastname: Inamdar
authors.affiliation: R&D Centre, Natural Remedies Pvt. Ltd., Plot No. 5B, Veerasandra Industrial Area, Bangalore, 560100, India.
authors.forename: Shekhar M
authors.initials: SM
authors.lastname: Dethe
authors.affiliation: R&D Centre, Natural Remedies Pvt. Ltd., Plot No. 5B, Veerasandra Industrial Area, Bangalore, 560100, India.
authors.forename: Giligar M
authors.initials: GM
authors.lastname: Gururaj
authors.affiliation: R&D Centre, Natural Remedies Pvt. Ltd., Plot No. 5B, Veerasandra Industrial Area, Bangalore, 560100, India.
authors.forename: Rohitash
authors.initials: R
authors.lastname: Jamwal
authors.affiliation: R&D Centre, Natural Remedies Pvt. Ltd., Plot No. 5B, Veerasandra Industrial Area, Bangalore, 560100, India.
authors.forename: Anirban
authors.initials: A
authors.lastname: Bhaskar
authors.affiliation: R&D Centre, Natural Remedies Pvt. Ltd., Plot No. 5B, Veerasandra Industrial Area, Bangalore, 560100, India.
authors.forename: Deepak
authors.initials: D
authors.lastname: Mundkinajeddu
authors.affiliation: R&D Centre, Natural Remedies Pvt. Ltd., Plot No. 5B, Veerasandra Industrial Area, Bangalore, 560100, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Agarwal
mesh_terms: D000818:Animals; D001046:Aphrodisiacs / Q000494:pharmacology*; D062186:Arterial Pressure / Q000187:drug effects; D004851:Epoxide Hydrolases / Q000037:antagonists & inhibitors / Q000502:physiology*; D005260:Female; D007427:Intracranial Pressure / Q000187:drug effects; D008297:Male; D029588:Moringa oleifera; D010410:Penile Erection / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats
chemical_list: D001046:Aphrodisiacs; D010936:Plant Extracts; D004851:Epoxide Hydrolases
keywords: CCSM; ICP; Moringa oleifera; erectile dysfunction; sEH