title: Apoptotic Cell Death Induced by ofLBP6A, Lipopolysaccharide Binding Protein Model Peptide, Derived from Paralichthy olivaceus on MKN-28 Cells.
abstract: The aim of this study was to evaluate the anti-cancer effects of lipopolysaccharide binding protein (LBP) analogs derived from the marine resource Paralichthy olivaceus on MKN-28 gastric cancer cells. Five LBP analogs were used: ofLBP1N, ofLBP2A, ofLBP4N, ofLBP5A, and ofLBP6A. ofLBP6A induced cell death of MKN-28 cells at a concentration of 40 μM. While the anti-proliferation effects ofLBP6A showed on MKN-28 cells at concentration of 40 μM, it did not affect non-cancerous HEK-293 cells at the same concentration. The mechanism study showed that ofLBP6A lead to the inhibition of cell proliferation by apoptosis along with morphological changes. The phosphorylation of Fas associated death domain (FADD) as well as the expressions of cleaved caspase-8, -7, and -3 were increased by ofLBP6A treatment. Increased the expression level of cleaved caspase-3 was confirmed by immunofluorescence staining. The expressions of Bid, Bax, and cytochrome C were also increased by the treatment. However, the expressions of cellular FLICE (FADD-like IL-1β-converting enzyme)-inhibitory protein (FLIP), Bcl-XL, and Bcl-2 were decreased by ofLBP6A treatment. The results of this study were the first to demonstrate the apoptotic anti-cancer effects of ofLBP6A, derived from P. olivavaceus on gastric cancer cells.
authors.affiliation: Department of Microbiology, College of Natural Sciences, Pukyung National University, Busan, 48513, Republic of Korea.
authors.forename: Chang-Won
authors.initials: CW
authors.lastname: Kang
authors.affiliation: Department of Microbiology, College of Natural Sciences, Pukyung National University, Busan, 48513, Republic of Korea.
authors.forename: Nan-Hee
authors.initials: NH
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, College of Fisheries Sciences, Pukyung National University, Busan, 48513, Republic of Korea.
authors.forename: Nam Gyu
authors.initials: NG
authors.lastname: Park
authors.affiliation: Department of Microbiology, College of Natural Sciences, Pukyung National University, Busan, 48513, Republic of Korea.
authors.forename: Gun-Do
authors.initials: GD
authors.lastname: Kim
mesh_terms: D000209:Acute-Phase Proteins / Q000494:pharmacology*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D002352:Carrier Proteins / Q000494:pharmacology*; D045744:Cell Line, Tumor; D045304:Cytochromes c / Q000378:metabolism; D005399:Fishes; D006801:Humans; D008562:Membrane Glycoproteins / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology*; D000099231:Lipopolysaccharide-Binding Protein
chemical_list: D000209:Acute-Phase Proteins; D000970:Antineoplastic Agents; D051017:Apoptosis Regulatory Proteins; D002352:Carrier Proteins; D045304:Cytochromes c; D008562:Membrane Glycoproteins; D010455:Peptides; D000099231:Lipopolysaccharide-Binding Protein
keywords: MKN-28 cells; apoptosis; caspase; gastric cancer; lipopolysaccharide binding protein (LBP)