title: A novel viral RNA helicase with an independent translation enhancement activity.
abstract: RNA helicases have not been identified among negative sense RNA viruses. In this study, it is shown that Nonstructural protein (NSs) of Groundnut bud necrosis virus (GBNV) acts as a Mg(2+) - and ATP-dependent bipolar RNA helicase. Biophysical and biochemical analysis of the deletion mutants (NΔ124 NSs, CΔ80 NSs) revealed that both the N- and C-terminal residues are required for substrate binding, oligomerization and helicase activity, but are dispensable for ATPase activity. Interestingly, NSs could enhance the translation of RNA (~ 10-fold) independent of its helicase activity. This is the first report of a RNA helicase from negative strand RNA viruses.
authors.affiliation: Department of Biochemistry, Indian Institute of Science, Bangalore, India.
authors.forename: Ambily
authors.initials: A
authors.lastname: Abraham
authors.affiliation: Department of Biochemistry, Indian Institute of Science, Bangalore, India.
authors.forename: Handanahal S
authors.initials: HS
authors.lastname: Savithri
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000595:Amino Acid Sequence; D055592:Biophysical Phenomena; D050505:Mutant Proteins / Q000302:isolation & purification; D010942:Plant Viruses / Q000201:enzymology*; D014176:Protein Biosynthesis; D020365:RNA Helicases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism; D017384:Sequence Deletion; D020349:Surface Plasmon Resonance; D017361:Viral Nonstructural Proteins / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D050505:Mutant Proteins; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins; D000251:Adenosine Triphosphatases; D020365:RNA Helicases
keywords: Groundnut bud necrosis virus; bipolar RNA helicase; nonstructural protein; translation enhancer