title: Succinic acid production from duckweed (Landoltia punctata) hydrolysate by batch fermentation of Actinobacillus succinogenes GXAS137.
abstract: Duckweed is potentially an ideal succinic acid (SA) feedstock due to its high proportion of starch and low lignin content. Pretreatment methods, substrate content and nitrogen source were investigated to enhance the bioconversion of duckweed to SA and to reduce the costs of production. Results showed that acid hydrolysis was an effective pretreatment method because of its high SA yield. The optimum substrate concentration was 140g/L. The optimum substrate concentration was 140g/L. Corn steep liquor powder could be considered a feasible and inexpensive alternative to yeast extract as a nitrogen source. Approximately 57.85g/L of SA was produced when batch fermentation was conducted in a 1.3L stirred bioreactor. Therefore, inexpensive duckweed can be a promising feedstock for the economical and efficient production of SA through fermentation by Actinobacillus succinogenes GXAS137.
authors.affiliation: Guangxi Key Laboratory of Subtropical Bio-resource Conservation and Utilization, College of Life Science and Technology, Guangxi University, Nanning, Guangxi 530005, China; National Non-grain Bio-energy Engineering Research Center, Guangxi Academy of Sciences, Nanning, Guangxi 530007, China.
authors.forename: Naikun
authors.initials: N
authors.lastname: Shen
authors.affiliation: Guangxi Key Laboratory of Subtropical Bio-resource Conservation and Utilization, College of Life Science and Technology, Guangxi University, Nanning, Guangxi 530005, China; National Non-grain Bio-energy Engineering Research Center, Guangxi Academy of Sciences, Nanning, Guangxi 530007, China.
authors.forename: Qingyan
authors.initials: Q
authors.lastname: Wang
authors.affiliation: National Non-grain Bio-energy Engineering Research Center, Guangxi Academy of Sciences, Nanning, Guangxi 530007, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Guangxi Key Laboratory of Subtropical Bio-resource Conservation and Utilization, College of Life Science and Technology, Guangxi University, Nanning, Guangxi 530005, China; National Non-grain Bio-energy Engineering Research Center, Guangxi Academy of Sciences, Nanning, Guangxi 530007, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Guangxi Key Laboratory of Subtropical Bio-resource Conservation and Utilization, College of Life Science and Technology, Guangxi University, Nanning, Guangxi 530005, China; National Non-grain Bio-energy Engineering Research Center, Guangxi Academy of Sciences, Nanning, Guangxi 530007, China.
authors.forename: Siming
authors.initials: S
authors.lastname: Liao
authors.affiliation: National Non-grain Bio-energy Engineering Research Center, Guangxi Academy of Sciences, Nanning, Guangxi 530007, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: National Non-grain Bio-energy Engineering Research Center, Guangxi Academy of Sciences, Nanning, Guangxi 530007, China.
authors.forename: Qixia
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Guangxi Key Laboratory of Subtropical Bio-resource Conservation and Utilization, College of Life Science and Technology, Guangxi University, Nanning, Guangxi 530005, China.
authors.forename: Liqin
authors.initials: L
authors.lastname: Du
authors.affiliation: Guangxi Key Laboratory of Subtropical Bio-resource Conservation and Utilization, College of Life Science and Technology, Guangxi University, Nanning, Guangxi 530005, China; National Non-grain Bio-energy Engineering Research Center, Guangxi Academy of Sciences, Nanning, Guangxi 530007, China. Electronic address: rbhuang@gxas.cn.
authors.forename: Ribo
authors.initials: R
authors.lastname: Huang
mesh_terms: D000188:Actinobacillus / Q000378:metabolism*; D029064:Araceae / Q000737:chemistry / Q000378:metabolism; D019149:Bioreactors / Q000382:microbiology*; D005285:Fermentation; D006868:Hydrolysis; D009584:Nitrogen / Q000378:metabolism; D019802:Succinic Acid / Q000032:analysis / Q000378:metabolism; D003313:Zea mays
chemical_list: D019802:Succinic Acid; D009584:Nitrogen
keywords: Actinobacillus succinogenes; Corn steep liquor powder; Duckweed; Pretreatment; Succinic acid