title: In vitro studies of the antiherpetic effect of photodynamic therapy.
abstract: The number of viral infection cases in the Department of Gynecology and Obstetrics has tended to increase over last few years. Viruses form herpesvirus and cytomegalovirus families are associated with an increased risk for recurrent pregnancy loss. Photodynamic therapy (PDT) is a promising new approach to treat viral infections in which viral particles are inactivated. It exhibits great therapeutic potential, particularly among this group of patients. This study examined the use of PDT to treat herpesvirus infection (HVI) using an in vitro model. In this study, we used the Vero сell lineage as a suitable model of HVI, strains of HSV-1 (strain VR-3) and HSV-2 (strain MS) obtained from The National Virus Collection (London, UK), the photosensitizer Fotoditazine (Veta-Grand, Russia), an AFS physiotherapeutic device (Polironic Corporation, Russia). Laser light irradiation and the photosensitizer had different cytotoxic effects on the Vero cell cultures depending on the doses used. The optimal laser light and photosensitizer doses were determined. PDT had an antiviral effect on an in vitro model of HVI in cell culture. PDT has been shown to be effective treatment for HVI in vitro, leading to a reliable decrease of viral titer.
authors.affiliation: Mechnikov Scientific Research Institute of Vaccines and Serums, Maly Kazeynny per., 5A, 105064, Moscow, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Zverev
authors.affiliation: Department of Obstetrics and Gynecology of Medical Faculty, Pyrogov Russian National Research Medical University, Str. Ostrovityanova, 1, 117997, Moscow, Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Makarov
authors.affiliation: Department of Obstetrics and Gynecology of Medical Faculty, Pyrogov Russian National Research Medical University, Str. Ostrovityanova, 1, 117997, Moscow, Russia.
authors.forename: A Z
authors.initials: AZ
authors.lastname: Khashukoeva
authors.affiliation: Mechnikov Scientific Research Institute of Vaccines and Serums, Maly Kazeynny per., 5A, 105064, Moscow, Russia.
authors.forename: O A
authors.initials: OA
authors.lastname: Svitich
authors.affiliation: Department of Obstetrics and Gynecology of Medical Faculty, Pyrogov Russian National Research Medical University, Str. Ostrovityanova, 1, 117997, Moscow, Russia.
authors.forename: Y E
authors.initials: YE
authors.lastname: Dobrokhotova
authors.affiliation: Department of Obstetrics and Gynecology of Medical Faculty, Pyrogov Russian National Research Medical University, Str. Ostrovityanova, 1, 117997, Moscow, Russia. Markova.eleonora@mail.ru.
authors.forename: E A
authors.initials: EA
authors.lastname: Markova
authors.affiliation: Mechnikov Scientific Research Institute of Vaccines and Serums, Maly Kazeynny per., 5A, 105064, Moscow, Russia.
authors.forename: P A
authors.initials: PA
authors.lastname: Labginov
authors.affiliation: Department of Obstetrics and Gynecology of Medical Faculty, Pyrogov Russian National Research Medical University, Str. Ostrovityanova, 1, 117997, Moscow, Russia.
authors.forename: S A
authors.initials: SA
authors.lastname: Khlinova
authors.affiliation: Mechnikov Scientific Research Institute of Vaccines and Serums, Maly Kazeynny per., 5A, 105064, Moscow, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Shulenina
authors.affiliation: Department of Immunology, Pyrogov Russian National Research Medical University, Str. Ostrovityanova, 1, 117997, Moscow, Russia.
authors.forename: L V
authors.initials: LV
authors.lastname: Gankovskaya
mesh_terms: D005260:Female; D018259:Herpesvirus 1, Human / Q000528:radiation effects*; D018258:Herpesvirus 2, Human / Q000528:radiation effects*; D006801:Humans; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000627:therapeutic use*
chemical_list: D017319:Photosensitizing Agents
keywords: Fotoditazine; Herpes simplex virus; Photodynamic therapy; Photosensitizer; Recurrent pregnancy loss