title: Silencing of Prrx1b suppresses cellular proliferation, migration, invasion and epithelial-mesenchymal transition in triple-negative breast cancer.
abstract: Triple-negative breast cancer (TNBC) is a highly aggressive tumour subtype associated with poor prognosis. The mechanisms involved in TNBC progression remains largely unknown. To date, there are no effective therapeutic targets for this tumour subtype. Paired-related homeobox 1b (Prrx1b), one of major isoforms of Prrx1, has been identified as a new epithelial-mesenchymal transition (EMT) inducer. However, the function of Prrx1b in TNBC has not been elucidated. In this study, we found that Prrx1b was significantly up-regulated in TNBC and associated with tumour size and vascular invasion of breast cancer. Silencing of Prrx1b suppressed the proliferation, migration and invasion of basal-like cancer cells. Moreover, silencing of Prrx1b prevented Wnt/β-catenin signaling pathway and induced the mesenchymal-epithelial transition (MET). Taken together, our data indicated that Prrx1b may be an important regulator of EMT in TNBC cells and a new therapeutic target for interventions against TNBC invasion and metastasis.
authors.affiliation: Center of Diagnosis and Treatment of Breast Disease, The Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Zhi-Dong
authors.initials: ZD
authors.lastname: Lv
authors.affiliation: Departments of Child Health Care, The Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Zhao-Chuan
authors.initials: ZC
authors.lastname: Yang
authors.affiliation: Central Laboratory of Molecular Biology, The Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Xiang-Ping
authors.initials: XP
authors.lastname: Liu
authors.affiliation: Cerebrovascular Disease Research Institute, The Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Jin
authors.affiliation: Departments of Pediatric Surgery, The Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Dong
authors.affiliation: Center of Diagnosis and Treatment of Breast Disease, The Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Hui-Li
authors.initials: HL
authors.lastname: Qu
authors.affiliation: Center of Diagnosis and Treatment of Breast Disease, The Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Fu-Nian
authors.initials: FN
authors.lastname: Li
authors.affiliation: Center of Diagnosis and Treatment of Breast Disease, The Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Kong
authors.affiliation: Center of Diagnosis and Treatment of Breast Disease, The Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Sun
authors.affiliation: Center of Diagnosis and Treatment of Breast Disease, The Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Jiao-Jiao
authors.initials: JJ
authors.lastname: Zhao
authors.affiliation: Center of Diagnosis and Treatment of Breast Disease, The Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Wang
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D048430:Cell Shape / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D051379:Mice; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D064726:Triple Negative Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D015854:Up-Regulation / Q000235:genetics; D014746:Vimentin / Q000378:metabolism; D060449:Wnt Signaling Pathway / Q000235:genetics
chemical_list: D015820:Cadherins; D018398:Homeodomain Proteins; C082157:PRRX1 protein, human; D014746:Vimentin
keywords: epithelial-mesenchymal transition; invasion; paired-related homeobox 1b; proliferation; triple-negative breast cancer