title: Cytotoxic Effect of Erythroxylum suberosum Combined with Radiotherapy in Head and Neck Cancer Cell Lines.
abstract: The mouth and oropharynx cancer is the 6th most common type of cancer in the world. The treatment may involve surgery, chemotherapy and radiotherapy. More than 50% of drugs against cancer were isolated from natural sources, such as Catharanthus roseus and epipodophyllotoxin, isolated from Podophyllum. The biggest challenge is to maximize the control of the disease, while minimizing morbidity and toxicity to the surrounding normal tissues. The Erythroxylum suberosum is a common plant in the Brazilian Cerrado biome and is popularly known as "cabelo-de-negro". The objective of this study was to evaluate the cytotoxic activity of Erythroxylum suberosum plant extracts of the Brazilian Cerrado biome associated with radiotherapy in human cell lines of oral and hypopharynx carcinomas. Cells were treated with aqueous, ethanolic and hexanic extracts of Erythroxylum suberosum and irradiated at 4 Gy, 6 Gy and 8 Gy. Cytotoxicity was evaluated by MTT assay and the absorbance was measured at 570 nm in a Beckman Counter reader. Cisplatin, standard chemotherapy, was used as positive control. The use of Erythroxylum suberosum extracts showed a possible radiosensitizing effect in vitro for head and neck cancer. The cytotoxicity effect in the cell lines was not selective and it is very similar to the effect of standard chemotherapy. The aqueous extract of Erythroxylum suberosum, combined with radiotherapy was the most cytotoxic extract to oral and hypopharynx carcinomas.
authors.affiliation: School of Dentistry, Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Taysa B C
authors.initials: TB
authors.lastname: Macedo
authors.affiliation: Faculty of Health Sciences, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: Silvia T
authors.initials: ST
authors.lastname: Elias
authors.affiliation: School of Dentistry, Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Hianne M
authors.initials: HM
authors.lastname: Torres
authors.affiliation: School of Dentistry, Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Fernanda Paula
authors.initials: FP
authors.lastname: Yamamoto-Silva
authors.affiliation: Faculty of Health Sciences, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: Dâmaris
authors.initials: D
authors.lastname: Silveira
authors.affiliation: Faculty of Health Sciences, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: Pérola O
authors.initials: PO
authors.lastname: Magalhães
authors.affiliation: Faculty of Health Sciences, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: Adriana
authors.initials: A
authors.lastname: Lofrano-Porto
authors.affiliation: Faculty of Health Sciences, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: Eliete N S
authors.initials: EN
authors.lastname: Guerra
authors.affiliation: School of Dentistry, Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Maria Alves G
authors.initials: MA
authors.lastname: Silva
mesh_terms: D000970:Antineoplastic Agents / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D003131:Combined Modality Therapy; D030921:Erythroxylaceae / Q000737:chemistry*; D006258:Head and Neck Neoplasms / Q000188:drug therapy* / Q000532:radiotherapy*; D006801:Humans; D010936:Plant Extracts / Q000627:therapeutic use*
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts