title: Effect of mitochondrial ascorbic acid synthesis on photosynthesis.
abstract: Ascorbic acid (AA) is synthesized in plant mitochondria through the oxidation of l-galactono-1,4-lactone (l-GalL) and then distributed to different cell compartments. AA-deficient Arabidopsis thaliana mutants (vtc2) and exogenous applications of l-GalL were used to generate plants with different AA content in their leaves. This experimental approach allows determining specific AA-dependent effects on carbon metabolism. No differences in O2 uptake, malic and citric acid and NADH content suggest that AA synthesis or accumulation did not affect mitochondrial activity; however, l-GalL treatment increased CO2 assimilation and photosynthetic electron transport rate in vtc2 (but not wt) leaves demonstrating a stimulation of photosynthesis after l-GalL treatment. Increased CO2 assimilation correlated with increased leaf stomatal conductance observed in l-GalL-treated vtc2 plants.
authors.affiliation: Instituto de Fisiología Vegetal (INFIVE), Facultades de Ciencias Agrarias y Forestales y de Ciencias Naturales y Museo, Universidad Nacional de La Plata, CCT-CONICET La Plata, cc 327, 1900, La Plata, Argentina.
authors.forename: M E
authors.initials: ME
authors.lastname: Senn
authors.affiliation: Instituto de Fisiología Vegetal (INFIVE), Facultades de Ciencias Agrarias y Forestales y de Ciencias Naturales y Museo, Universidad Nacional de La Plata, CCT-CONICET La Plata, cc 327, 1900, La Plata, Argentina.
authors.forename: G E
authors.initials: GE
authors.lastname: Gergoff Grozeff
authors.affiliation: Instituto de Fisiología Vegetal (INFIVE), Facultades de Ciencias Agrarias y Forestales y de Ciencias Naturales y Museo, Universidad Nacional de La Plata, CCT-CONICET La Plata, cc 327, 1900, La Plata, Argentina.
authors.forename: M L
authors.initials: ML
authors.lastname: Alegre
authors.affiliation: Instituto de Fisiología Vegetal (INFIVE), Facultades de Ciencias Agrarias y Forestales y de Ciencias Naturales y Museo, Universidad Nacional de La Plata, CCT-CONICET La Plata, cc 327, 1900, La Plata, Argentina.
authors.forename: F
authors.initials: F
authors.lastname: Barrile
authors.affiliation: Department of Earth and Environmental Sciences, Università degli Studi di Bari, Italy.
authors.forename: M C
authors.initials: MC
authors.lastname: De Tullio
authors.affiliation: Instituto de Fisiología Vegetal (INFIVE), Facultades de Ciencias Agrarias y Forestales y de Ciencias Naturales y Museo, Universidad Nacional de La Plata, CCT-CONICET La Plata, cc 327, 1900, La Plata, Argentina. Electronic address: carlos.bartoli@agro.unlp.edu.ar.
authors.forename: C G
authors.initials: CG
authors.lastname: Bartoli
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000502:physiology*; D001205:Ascorbic Acid / Q000096:biosynthesis*; D019069:Cell Respiration / Q000187:drug effects; D003683:Dehydroascorbic Acid / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D007783:Lactones / Q000494:pharmacology; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D010788:Photosynthesis / Q000187:drug effects; D054046:Plant Stomata / Q000187:drug effects / Q000502:physiology; D011725:Pyridines / Q000494:pharmacology; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism; D013400:Sugar Acids / Q000494:pharmacology
chemical_list: D007783:Lactones; D011725:Pyridines; D013400:Sugar Acids; C534014:galactono-1,4-lactone; D012273:Ribulose-Bisphosphate Carboxylase; D005978:Glutathione; C023666:pyridine; D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid
keywords: Ascorbic acid; Photosynthesis; Respiration; Stomata