title: Anti-Streptococcus mutans property of a chitosan: Containing resin sealant.
abstract: OBJECTIVE
  This study sought to assess the inhibitory effect of chitosan-containing sealants against Streptococcus mutans.
  
  
  METHODS
  The antibacterial activity of the resin sealant was evaluated by direct contact test following the addition of 0, 1, 2, 3, 4, and 5 wt% chitosan. At 3, 6, 9, 24 and 48 h, 1 and 3 months, 10 μl of the microbial suspension in contact with resin sealant was cultured to count the number of colonies. Data were analyzed by one-way one-way analysis of variance (ANOVA), repeated measures ANOVA, and Scheffe test.
  
  
  RESULTS
  The minimum inhibitory concentration of chitosan against S. mutans was 2 wt%. At 3 h, bacterial count in the presence of 2-5 wt% chitosan was significantly lower than that at 0 and 1 wt% (P < 0.05). However, this difference in bacterial count between 2 and 3 wt% chitosan and between 4 and 5 wt% chitosan was not significant. At 6 h, the difference in bacterial count between 3 and 4 wt% chitosan was not significant, whereas the remaining groups were significantly different in terms of bacterial count at this time (P < 0.05). At the remaining time points, significant differences were found between 2 wt% chitosan and higher concentrations (P < 0.05).
  
  
  CONCLUSIONS
  Sealants containing 2-5 wt% chitosan show an antimicrobial property that is intensified by increasing the concentration of chitosan.
authors.affiliation: Department of Microbiology, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Ramazan
authors.initials: R
authors.lastname: Rajabnia
authors.affiliation: Department of Microbiology, Dental Material Research Center, Babol, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Ghasempour
authors.affiliation: Department of Pediatrics, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Samane
authors.initials: S
authors.lastname: Gharekhani
authors.affiliation: Student's Research Committee, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Sepide
authors.initials: S
authors.lastname: Gholamhoseinnia
authors.affiliation: Department of Pediatrics, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Sepide
authors.initials: S
authors.lastname: Soroorhomayoon
keywords: Antimicrobial property; Streptococcus mutans; chitosan; direct contact test; resin sealant