title: Detection of four human polyomaviruses (MCPyV, HPyV6, HPyV7 and TSPyV) in cervical specimens from HIV-infected and HIV-uninfected women.
abstract: OBJECTIVE
  To investigate the presence of recently discovered human polyomaviruses in cervical specimens collected from African and French women, in relation to HIV serostatus, high-risk human papillomavirus infection (HR-HPV) and cervical disease.
  
  
  METHODS
  Cervical specimens were collected from 140 HIV-1-seropositive African women and 50 HIV-seronegative French women. Presence of Merkel cell polyomavirus (MCPyV), human polyomavirus 6 (HPyV6), human polyomavirus 7 (HPyV7) and trichodysplasia spinulosa-associated polyomavirus (TSPyV) was detected by real-time PCR, and presence of HR-HPV DNA by Hybrid Capture 2 assay with subsequent HPV genotyping using the INNO-LiPA HPV Genotyping Extra assay. Cervical biopsies were analysed by histopathology.
  
  
  RESULTS
  The detection rates were 55.3%, 3.2%, 2.1% and 0% for MCPyV, HPyV6, HPyV7 and TSPyV, respectively, with no significant difference by population. The MCPyV viral load ranged from 14 to 210 DNA copies/106cells (median, 80 DNA copies/106cells), with no difference between women with and without cervical precancerous lesions. There was no association between detection of human polyomaviruses in cervical specimens and geographical origin/HIV serostatus, HR-HPV coinfection or precancerous cervical lesions.
  
  
  CONCLUSIONS
  These observations argue against a possible role of MCPyV as a cofactor in HPV-induced carcinogenesis. MCPyV and, to a lesser extent, HPyV6 and HPyV7 might belong to the female genital tract microbiota.
authors.affiliation: INSERM U.1058, Université de Montpellier, Montpellier, France.
authors.forename: Pratt
authors.initials: P
authors.lastname: Kolia-Diafouka
authors.affiliation: INSERM U.1058, Université de Montpellier, Montpellier, France.|Pôle Biologie-Pathologie, Centre Hospitalier Universitaire (CHU), Montpellier, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Foulongne
authors.affiliation: INSERM U.1058, Université de Montpellier, Montpellier, France.|Pôle Biologie-Pathologie, Centre Hospitalier Universitaire (CHU), Montpellier, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Boulle
authors.affiliation: INSERM U.1058, Université de Montpellier, Montpellier, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Ngou
authors.affiliation: London School of Hygiene and Tropical Medicine, London, UK.
authors.forename: Helen
authors.initials: H
authors.lastname: Kelly
authors.affiliation: Centre de Recherche Internationale pour la Santé (CRIS), Université de Ouagadougou, Ouagadougou, Burkina Faso.
authors.forename: Bernard
authors.initials: B
authors.lastname: Sawadogo
authors.affiliation: Wits Reproductive Health and HIV Institute, School of Clinical Medicine, University of the Witwatersrand, Johannesburg, South Africa.
authors.forename: Sinead
authors.initials: S
authors.lastname: Delany-Moretlwe
authors.affiliation: London School of Hygiene and Tropical Medicine, London, UK.|Wits Reproductive Health and HIV Institute, School of Clinical Medicine, University of the Witwatersrand, Johannesburg, South Africa.
authors.forename: Philippe
authors.initials: P
authors.lastname: Mayaud
authors.affiliation: INSERM U.1058, Université de Montpellier, Montpellier, France.|Pôle Biologie-Pathologie, Centre Hospitalier Universitaire (CHU), Montpellier, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Segondy
keywords: CERVICAL NEOPLASIA; HIV WOMEN; VIROLOGY CLINICAL