title: Low Genetic Quality Alters Key Dimensions of the Mutational Spectrum.
abstract: Mutations affect individual health, population persistence, adaptation, diversification, and genome evolution. There is evidence that the mutation rate varies among genotypes, but the causes of this variation are poorly understood. Here, we link differences in genetic quality with variation in spontaneous mutation in a Drosophila mutation accumulation experiment. We find that chromosomes maintained in low-quality genetic backgrounds experience a higher rate of indel mutation and a lower rate of gene conversion in a manner consistent with condition-based differences in the mechanisms used to repair DNA double strand breaks. These aspects of the mutational spectrum were also associated with body mass, suggesting that the effect of genetic quality on DNA repair was mediated by overall condition, and providing a mechanistic explanation for the differences in mutational fitness decline among these genotypes. The rate and spectrum of substitutions was unaffected by genetic quality, but we find variation in the probability of substitutions and indels with respect to several aspects of local sequence context, particularly GC content, with implications for models of molecular evolution and genome scans for signs of selection. Our finding that the chances of mutation depend on genetic context and overall condition has important implications for how sequences evolve, the risk of extinction, and human health.
authors.affiliation: Department of Ecology and Evolutionary Biology, University of Toronto, Toronto, Ontario, Canada.|Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Nathaniel P
authors.initials: NP
authors.lastname: Sharp
authors.affiliation: Department of Ecology and Evolutionary Biology, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Aneil F
authors.initials: AF
authors.lastname: Agrawal
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster; D005260:Female; D056084:Genetic Fitness; D008297:Male; D000067552:Mutation Accumulation; D059645:Mutation Rate