title: POLYPHASIC CHARACTERIZATION OF DOLICHOSPERMUM SPP. AND SPHAEROSPERMOPSIS SPP. (NOSTOCALES, CYANOBACTERIA): MORPHOLOGY, 16S rRNA GENE SEQUENCES AND FATTY ACID AND SECONDARY METABOLITE PROFILES(1).
abstract: The genera Dolichospermum (Ralfs ex Bornet et Flahault) Wacklin, L. Hoffm. et Komárek and Sphaerospermopsis Zapomělová, Jezberová, Hrouzek, Hisem, K. Řeháková et Komárk.-Legn. represent a highly diversified group of planktonic cyanobacteria that have been recently separated from the traditional genus Anabaena Bory ex Bornet et Flahault. In this study, morphological diversity, phylogeny of the 16S rRNA gene, production of fatty acids, and secondary metabolite profiles were evaluated in 33 strains of 14 morphospecies isolated from the Czech Republic. Clustering of the strains based on 16S rRNA gene sequences corresponded to wider groups of species in terms of morphology. The overall secondary metabolite and fatty acid profiles, however, were not correlated to each other and neither were they correlated to the 16S rRNA phylogeny nor the morphology of the strains. Nevertheless, a minor part of the detected secondary metabolites (19% of all compounds) was present only in close relatives and can be thus considered as autapomorphic features.
authors.affiliation: Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Microbiology, AS CR, Department of Autotrophic Microorganisms, Opatovický mlýn, CZ-379 81 Třeboň, Czech Republic Institute of Physical Biology, Zámek 136, CZ-37333 Nové Hrady, Czech Republic University of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech RepublicInstitute of Microbiology, AS CR, Vídeňská 1083, CZ-14220 Prague, Czech RepublicBiology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Botany, AS CR, Dukelská 135, CZ-37982 Třeboň, Czech Republic Institute of Microbiology, AS CR, Department of Autotrophic Microorganisms, Opatovický mlýn, CZ-379 81 Třeboň, Czech Republic University of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech Republic Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Botany, AS CR, Dukelská 135, CZ-37982 Třeboň, Czech Republic.
authors.forename: Eliška
authors.initials: E
authors.lastname: Zapomělová
authors.affiliation: Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Microbiology, AS CR, Department of Autotrophic Microorganisms, Opatovický mlýn, CZ-379 81 Třeboň, Czech Republic Institute of Physical Biology, Zámek 136, CZ-37333 Nové Hrady, Czech Republic University of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech RepublicInstitute of Microbiology, AS CR, Vídeňská 1083, CZ-14220 Prague, Czech RepublicBiology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Botany, AS CR, Dukelská 135, CZ-37982 Třeboň, Czech Republic Institute of Microbiology, AS CR, Department of Autotrophic Microorganisms, Opatovický mlýn, CZ-379 81 Třeboň, Czech Republic University of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech Republic Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Botany, AS CR, Dukelská 135, CZ-37982 Třeboň, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Hrouzek
authors.affiliation: Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Microbiology, AS CR, Department of Autotrophic Microorganisms, Opatovický mlýn, CZ-379 81 Třeboň, Czech Republic Institute of Physical Biology, Zámek 136, CZ-37333 Nové Hrady, Czech Republic University of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech RepublicInstitute of Microbiology, AS CR, Vídeňská 1083, CZ-14220 Prague, Czech RepublicBiology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Botany, AS CR, Dukelská 135, CZ-37982 Třeboň, Czech Republic Institute of Microbiology, AS CR, Department of Autotrophic Microorganisms, Opatovický mlýn, CZ-379 81 Třeboň, Czech Republic University of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech Republic Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Botany, AS CR, Dukelská 135, CZ-37982 Třeboň, Czech Republic.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Řezanka
authors.affiliation: Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Microbiology, AS CR, Department of Autotrophic Microorganisms, Opatovický mlýn, CZ-379 81 Třeboň, Czech Republic Institute of Physical Biology, Zámek 136, CZ-37333 Nové Hrady, Czech Republic University of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech RepublicInstitute of Microbiology, AS CR, Vídeňská 1083, CZ-14220 Prague, Czech RepublicBiology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Botany, AS CR, Dukelská 135, CZ-37982 Třeboň, Czech Republic Institute of Microbiology, AS CR, Department of Autotrophic Microorganisms, Opatovický mlýn, CZ-379 81 Třeboň, Czech Republic University of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech Republic Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Botany, AS CR, Dukelská 135, CZ-37982 Třeboň, Czech Republic.
authors.forename: Jitka
authors.initials: J
authors.lastname: Jezberová
authors.affiliation: Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Microbiology, AS CR, Department of Autotrophic Microorganisms, Opatovický mlýn, CZ-379 81 Třeboň, Czech Republic Institute of Physical Biology, Zámek 136, CZ-37333 Nové Hrady, Czech Republic University of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech RepublicInstitute of Microbiology, AS CR, Vídeňská 1083, CZ-14220 Prague, Czech RepublicBiology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Botany, AS CR, Dukelská 135, CZ-37982 Třeboň, Czech Republic Institute of Microbiology, AS CR, Department of Autotrophic Microorganisms, Opatovický mlýn, CZ-379 81 Třeboň, Czech Republic University of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech Republic Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Botany, AS CR, Dukelská 135, CZ-37982 Třeboň, Czech Republic.
authors.forename: Klára
authors.initials: K
authors.lastname: Řeháková
authors.affiliation: Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Microbiology, AS CR, Department of Autotrophic Microorganisms, Opatovický mlýn, CZ-379 81 Třeboň, Czech Republic Institute of Physical Biology, Zámek 136, CZ-37333 Nové Hrady, Czech Republic University of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech RepublicInstitute of Microbiology, AS CR, Vídeňská 1083, CZ-14220 Prague, Czech RepublicBiology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Botany, AS CR, Dukelská 135, CZ-37982 Třeboň, Czech Republic Institute of Microbiology, AS CR, Department of Autotrophic Microorganisms, Opatovický mlýn, CZ-379 81 Třeboň, Czech Republic University of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech Republic Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Botany, AS CR, Dukelská 135, CZ-37982 Třeboň, Czech Republic.
authors.forename: Daniel
authors.initials: D
authors.lastname: Hisem
authors.affiliation: Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Microbiology, AS CR, Department of Autotrophic Microorganisms, Opatovický mlýn, CZ-379 81 Třeboň, Czech Republic Institute of Physical Biology, Zámek 136, CZ-37333 Nové Hrady, Czech Republic University of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech RepublicInstitute of Microbiology, AS CR, Vídeňská 1083, CZ-14220 Prague, Czech RepublicBiology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Botany, AS CR, Dukelská 135, CZ-37982 Třeboň, Czech Republic Institute of Microbiology, AS CR, Department of Autotrophic Microorganisms, Opatovický mlýn, CZ-379 81 Třeboň, Czech Republic University of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech Republic Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Botany, AS CR, Dukelská 135, CZ-37982 Třeboň, Czech Republic.
authors.forename: Jaroslava
authors.initials: J
authors.lastname: Komárková
keywords: 16S rRNA gene; Anabaena; Dolichospermum; Sphaerospermopsis; cyanobacteria; fatty acids; morphology; phytoplankton; secondary metabolites; taxonomy