title: Genome-wide Scanning and Characterization of Sorghum bicolor L. Heat Shock Transcription Factors.
abstract: A genome-wide scanning of Sorghum bicolor resulted in the identification of 25 SbHsf genes. Phylogenetic analysis shows the ortholog genes that are clustered with only rice, representing a common ancestor. Promoter analysis revealed the identification of different cis-acting elements that are responsible for abiotic as well as biotic stresses. Hsf domains like DBD, NLS, NES, and AHA have been analyzed for their sequence similarity and functional characterization. Tissue specific expression patterns of Hsfs in different tissues like mature embryo, seedling, root, and panicle were studied using real-time PCR. While Hsfs4 and 22 are highly expressed in panicle, 4 and 9 are expressed in seedlings. Sorghum plants were exposed to different abiotic stress treatments but no expression of any Hsf was observed when seedlings were treated with ABA. High level expression of Hsf1 was noticed during high temperature as well as cold stresses, 4 and 6 during salt and 5, 6, 10, 13, 19, 23 and 25 during drought stress. This comprehensive analysis of SbHsf genes will provide an insight on how these genes are regulated in different tissues and also under different abiotic stresses and help to determine the functions of Hsfs during drought and temperature stress tolerance.
authors.affiliation: Department of Genetics, Osmania University, Hyderabad 500 007, India;
authors.forename: M
authors.initials: M
authors.lastname: Nagaraju
authors.affiliation: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru-502324, Hyderabad, India.
authors.forename: Palakolanu Sudhakar
authors.initials: PS
authors.lastname: Reddy
authors.affiliation: Department of Genetics, Osmania University, Hyderabad 500 007, India;
authors.forename: S Anil
authors.initials: SA
authors.lastname: Kumar
authors.affiliation: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru-502324, Hyderabad, India.
authors.forename: Rakesh K
authors.initials: RK
authors.lastname: Srivastava
authors.affiliation: Department of Genetics, Osmania University, Hyderabad 500 007, India;
authors.forename: P B Kavi
authors.initials: PB
authors.lastname: Kishor
authors.affiliation: Department of Genetics, Osmania University, Hyderabad 500 007, India;
authors.forename: D Manohar
authors.initials: DM
authors.lastname: Rao
keywords: Abiotic stress.; Cis-acting elements; Heat shock transcription factors; Molecular chaperones; Paralogs; Phylogenetic analysis