title: Spinal Cord T-Cell Infiltration in the Rat Spared Nerve Injury Model: A Time Course Study.
abstract: The immune system is involved in the development of neuropathic pain. In particular, the infiltration of T-lymphocytes into the spinal cord following peripheral nerve injury has been described as a contributor to sensory hypersensitivity. We used the spared nerve injury (SNI) model of neuropathic pain in Sprague Dawley adult male rats to assess proliferation, and/or protein/gene expression levels for microglia (Iba1), T-lymphocytes (CD2) and cytotoxic T-lymphocytes (CD8). In the dorsal horn ipsilateral to SNI, Iba1 and BrdU stainings revealed microglial reactivity and proliferation, respectively, with different durations. Iba1 expression peaked at D4 and D7 at the mRNA and protein level, respectively, and was long-lasting. Proliferation occurred almost exclusively in Iba1 positive cells and peaked at D2. Gene expression observation by RT-qPCR array suggested that T-lymphocytes attracting chemokines were upregulated after SNI in rat spinal cord but only a few CD2/CD8 positive cells were found. A pronounced infiltration of CD2/CD8 positive T-cells was seen in the spinal cord injury (SCI) model used as a positive control for lymphocyte infiltration. Under these experimental conditions, we show early and long-lasting microglia reactivity in the spinal cord after SNI, but no lymphocyte infiltration was found.
authors.affiliation: Pain Center, Department of Anesthesiology, Lausanne University Hospital (CHUV) and University of Lausanne, 1011 Lausanne, Switzerland. christophe.gattlen@unil.ch.|Department of Fundamental Neurosciences, University of Lausanne, 1005 Lausanne, Switzerland. christophe.gattlen@unil.ch.
authors.forename: Christophe
authors.initials: C
authors.lastname: Gattlen
authors.affiliation: Pain Center, Department of Anesthesiology, Lausanne University Hospital (CHUV) and University of Lausanne, 1011 Lausanne, Switzerland.|Department of Fundamental Neurosciences, University of Lausanne, 1005 Lausanne, Switzerland. Nicolas.Piller@chuv.ch.
authors.forename: Christine B
authors.initials: CB
authors.lastname: Clarke
authors.affiliation: Pain Center, Department of Anesthesiology, Lausanne University Hospital (CHUV) and University of Lausanne, 1011 Lausanne, Switzerland. Nicolas.Piller@chuv.ch.|Department of Fundamental Neurosciences, University of Lausanne, 1005 Lausanne, Switzerland. Guylene.Kirschmann@unil.ch.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Piller
authors.affiliation: Pain Center, Department of Anesthesiology, Lausanne University Hospital (CHUV) and University of Lausanne, 1011 Lausanne, Switzerland. Guylene.Kirschmann@unil.ch.|Department of Fundamental Neurosciences, University of Lausanne, 1005 Lausanne, Switzerland. Marie.Pertin@unil.ch.
authors.forename: Guylène
authors.initials: G
authors.lastname: Kirschmann
authors.affiliation: Pain Center, Department of Anesthesiology, Lausanne University Hospital (CHUV) and University of Lausanne, 1011 Lausanne, Switzerland. Marie.Pertin@unil.ch.|Department of Fundamental Neurosciences, University of Lausanne, 1005 Lausanne, Switzerland. Isabelle.Decosterd@chuv.ch.
authors.forename: Marie
authors.initials: M
authors.lastname: Pertin
authors.affiliation: Pain Center, Department of Anesthesiology, Lausanne University Hospital (CHUV) and University of Lausanne, 1011 Lausanne, Switzerland. Isabelle.Decosterd@chuv.ch.|Department of Fundamental Neurosciences, University of Lausanne, 1005 Lausanne, Switzerland. rdg@biotelligences.com.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Decosterd
authors.affiliation: Pain Center, Department of Anesthesiology, Lausanne University Hospital (CHUV) and University of Lausanne, 1011 Lausanne, Switzerland. rdg@biotelligences.com.|Department of Fundamental Neurosciences, University of Lausanne, 1005 Lausanne, Switzerland. rdg@biotelligences.com.
authors.forename: Romain-Daniel
authors.initials: RD
authors.lastname: Gosselin
authors.affiliation: Pain Center, Department of Anesthesiology, Lausanne University Hospital (CHUV) and University of Lausanne, 1011 Lausanne, Switzerland. marc.suter@chuv.ch.|Department of Fundamental Neurosciences, University of Lausanne, 1005 Lausanne, Switzerland. marc.suter@chuv.ch.
authors.forename: Marc R
authors.initials: MR
authors.lastname: Suter
mesh_terms: D000818:Animals; D018801:CD2 Antigens / Q000235:genetics; D016827:CD8 Antigens / Q000235:genetics; D002135:Calcium-Binding Proteins / Q000235:genetics; D049109:Cell Proliferation; D018925:Chemokines / Q000276:immunology; D004195:Disease Models, Animal; D015870:Gene Expression; D008297:Male; D008840:Microfilament Proteins / Q000235:genetics; D017628:Microglia / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D009437:Neuralgia; D059348:Peripheral Nerve Injuries / Q000150:complications* / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000209:etiology* / Q000276:immunology / Q000503:physiopathology; D013601:T-Lymphocytes / Q000378:metabolism / Q000473:pathology / Q000502:physiology*
chemical_list: C100745:Aif1 protein, rat; D018801:CD2 Antigens; D016827:CD8 Antigens; D002135:Calcium-Binding Proteins; D018925:Chemokines; D008840:Microfilament Proteins
keywords: SCI (spinal cord injury); SNI (spared nerve injury); lymphocytes; microglia; neuropathic pain; peripheral nerve injury