title: miR-1934, downregulated in obesity, protects against low-grade inflammation in adipocytes.
abstract: It has been previously demonstrated that miR-1934 was specially regulated by adiponectin, an anti-inflammatory adipokine, in adipose tissue (AT) in vivo. Herein we investigated the role of miR1934 in the regulation of inflammatory response. Compared with chow-diet fed mice, miR1934 expression was down-regulated in epididymal AT of high-fat diet-induced obese mice. miR1934 expression was down-regulated as well in omental AT of obese subjects in comparison with lean subjects. The circulating miR-1934 was also lower in obese subjects and its levels were correlated negatively with body mass index (BMI), waist circumference (WC), low-density lipoprotein cholesterol (LDL-c), insulin resistance and high-sensitivity C-reactive protein (hs-CRP). The down-regulation of miR1934 in obesity was mimicked by TNF-α treatment of 3T3-L1 adipocytes. Moreover, overexpression of miR1934 suppressed the TNF-α-induced gene expression of interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1) in 3T3-L1 adipocytes. De novo formed AT in nude mice transplanted with 3T3-L1 preadipocytes overexpressing miR1934 displayed a reduction in IL-6, TNF-α, IL-1β and an enhancement in IL-10 gene expression when compared with transplant with 3T3-L1 preadipocytes overexpressing miR1934 scrambled control sequence. These results suggest that miR1934 is an important anti-inflammatory factor and may represent a novel mechanism for controlling AT inflammation.
authors.affiliation: Department of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, 400016 Chongqing, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, 400016 Chongqing, China.
authors.forename: Qifu
authors.initials: Q
authors.lastname: Li
authors.affiliation: Laboratory of Glucose and Lipid Metabolism, The First Affiliated Hospital of Chongqing Medical University, 400016 Chongqing, China.
authors.forename: Xiaoqiu
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Department of Nutrition and Food Science, Texas A&M University, College Station, TX, USA.
authors.forename: Chaodong
authors.initials: C
authors.lastname: Wu
authors.affiliation: Laboratory of Glucose and Lipid Metabolism, The First Affiliated Hospital of Chongqing Medical University, 400016 Chongqing, China.
authors.forename: Rufei
authors.initials: R
authors.lastname: Gao
authors.affiliation: Laboratory of Glucose and Lipid Metabolism, The First Affiliated Hospital of Chongqing Medical University, 400016 Chongqing, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Peng
authors.affiliation: Department of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, 400016 Chongqing, China.
authors.forename: Danting
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, 400016 Chongqing, China.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, 400016 Chongqing, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Du
authors.affiliation: Department of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, 400016 Chongqing, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, 400016 Chongqing, China.
authors.forename: Shumin
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, 400016 Chongqing, China.
authors.forename: Qianna
authors.initials: Q
authors.lastname: Zhen
authors.affiliation: Department of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, 400016 Chongqing, China. Electronic address: geqianqian@aliyun.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Ge
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D054392:Adipokines / Q000235:genetics / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D000886:Anthropometry; D002454:Cell Differentiation / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000235:genetics* / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D035683:MicroRNAs / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009765:Obesity / Q000097:blood / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D054392:Adipokines; C000623097:MIRN1934 microRNA, human; C000623098:MIRN1934 microRNA, mouse; D035683:MicroRNAs; D014409:Tumor Necrosis Factor-alpha
keywords: Adipocyte; Adipose tissue; Low-grade inflammation; Obesity; microRNA