title: Soft-shelled turtle eggs inhibit the formation of AGEs in the serum and skin of diabetic rats.
abstract: Although soft-shelled turtle eggs (STE) have been used as a folk medicine for revitalization and the prevention of lifestyle-related diseases, the scientific evidence to support the use of STE in this manner is scarce. To clarify the physiological evidence, STE was administered to diabetic rats and the inhibitory effects on the formation of advanced glycation end-products (AGEs), which are known to increase with the progression of lifestyle-related diseases, were examined. STE and citric acid were administered to diabetic rats for 3 months, and serum N (ε)-(carboxymethyl)lysine (CML) contents were measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Although the administration of STE did not affect the body weight, glycoalbumin or ketone body levels, it significantly reduced the serum level of CML. The accumulation of AGEs, which was measured by fluorescence intensity in the auricle skin and the lower gums, was also reduced by the administration of STE to a similar extent to that observed with citric acid. This report provides the first evidence that the oral administration of STE reduces the formation of AGEs, suggesting that one of the health effects of STE may be the inhibition of AGEs formation.
authors.affiliation: Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso-gun, Kumamoto 869-1404, Japan; Healthcare Business Development Department I, Medical and Healthcare Business Development Unit, Business Solution Company, SHARP Corporation, 2613-1 Ichinomoto-cho, Tenri, Nara 632-8567, Japan.
authors.forename: Mikihiro
authors.initials: M
authors.lastname: Yamanaka
authors.affiliation: Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso-gun, Kumamoto 869-1404, Japan.
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Shirakawa
authors.affiliation: Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso-gun, Kumamoto 869-1404, Japan.
authors.forename: Rei-Ichi
authors.initials: R
authors.lastname: Ohno
authors.affiliation: Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso-gun, Kumamoto 869-1404, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Shinagawa
authors.affiliation: Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso-gun, Kumamoto 869-1404, Japan.
authors.forename: Kota
authors.initials: K
authors.lastname: Hatano
authors.affiliation: Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso-gun, Kumamoto 869-1404, Japan.
authors.forename: Hikari
authors.initials: H
authors.lastname: Sugawa
authors.affiliation: Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso-gun, Kumamoto 869-1404, Japan; Department of Orthopaedic Surgery, Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan.
authors.forename: Shoutaro
authors.initials: S
authors.lastname: Arakawa
authors.affiliation: Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso-gun, Kumamoto 869-1404, Japan.
authors.forename: Chisato
authors.initials: C
authors.lastname: Furusawa
authors.affiliation: Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso-gun, Kumamoto 869-1404, Japan.
authors.forename: Mime
authors.initials: M
authors.lastname: Nagai
authors.affiliation: Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso-gun, Kumamoto 869-1404, Japan.
authors.forename: Ryoji
authors.initials: R
authors.lastname: Nagai
keywords: AGEs; CML; diabetes mellitus; oxidation; soft-shelled turtle eggs