title: Silk fibroin scaffold as a potential choice for female pelvic reconstruction: A study on the biocompatibility in abdominal wall, pelvic, and vagina.
abstract: The aim of this study was to compare the tissue reactions to silk fibroin scaffolds in the abdominal wall, vagina, and pelvic vesico-uterine of rats. Silk fibroin scaffolds were implanted subcutaneously in the abdominal, pelvic vesico-uterine space, and under the vaginal mucosa of 16 rats. The animals were euthanized at 2, 4, 8, and 12 weeks postoperatively. Hematoxylin and eosin staining was performed to evaluate cellular infiltration, the percentage of macrophages and granulocytes inside and around the scaffolds. The amounts of M1/M2 macrophages at the interface of scaffolds and host tissue were evaluated through an immunofluorescence assay. The degree of acute inflammation was similar among the three groups, and lasted no more than 4 weeks. A faster ingrowth of fibroblasts was found in the abdominally implanted silk fibroin scaffolds, while vaginal implanted scaffolds committed a slower tissue ingrowth rate and much more macrophages infiltration than the pelvic and abdominal group. However, a significantly higher amount of M2 cells were seen in the three groups. In general, silk fibroin has nice biocompatibility in the abdominal, vagina, and pelvic tissue, eliciting healthy tissue formation, and might be a potential choice for female pelvic reconstruction. Microsc. Res. Tech. 80:291-297, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Obstetrics and Gynecology, Peking University People's Hospital, Beijing, 100044, People's Republic of China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Chang
authors.affiliation: Department of Obstetrics and Gynecology, Peking University People's Hospital, Beijing, 100044, People's Republic of China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, Shandong Province, 250012, People's Republic of China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.affiliation: School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, People's Republic of China.
authors.forename: Xili
authors.initials: X
authors.lastname: Ding
authors.affiliation: School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, People's Republic of China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, Peking University People's Hospital, Beijing, 100044, People's Republic of China.
authors.forename: Jianliu
authors.initials: J
authors.lastname: Wang
mesh_terms: D034861:Abdominal Wall / Q000502:physiology*; D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000009:adverse effects / Q000138:chemical synthesis*; D005260:Female; D005349:Fibroins / Q000008:administration & dosage* / Q000009:adverse effects / Q000737:chemistry; D006098:Granulocytes / Q000276:immunology; D007249:Inflammation / Q000276:immunology; D008264:Macrophages / Q000166:cytology / Q000276:immunology; D010388:Pelvis / Q000502:physiology*; D016062:Porosity; D051381:Rats; D017207:Rats, Sprague-Dawley; D047011:Silk / Q000008:administration & dosage* / Q000009:adverse effects / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000009:adverse effects / Q000737:chemistry; D014621:Vagina / Q000502:physiology*
chemical_list: D001672:Biocompatible Materials; D047011:Silk; D005349:Fibroins
keywords: inflammation; macrophage; pelvic floor dysfunction; scaffold; silk fibroin