title: Peptide-incorporated 3D porous alginate scaffolds with enhanced osteogenesis for bone tissue engineering.
abstract: Good bioactivity and osteogenesis of three-dimensional porous alginate scaffolds (PAS) are critical for bone tissue engineering. In this work, alginate and bone-forming peptide-1 (BFP-1), derived from bone morphogenetic protein-7 (BMP-7), have been combined together (without carbodiimide chemistry treatment) to develop peptide-incorporated PAS (p-PAS) for promoting bone repairing ability. The mechanical properties and SEM images show no difference between pure PAS and p-PAS. The release kinetics of the labeled peptide with 6-carboxy tetramethyl rhodamine from the PAS matrix suggests that the peptide is released in a relatively sustained manner. In the cell experiment, p-PAS show higher cell adhesion, spreading, proliferation and alkaline phosphatase (ALP) activity than the pristine PAS group, indicating that the BFP-1 released from p-PAS could significantly promote the aggregation and differentiation of osteoblasts, especially at 10μg/mL of trapped peptide concentration (p-PAS-10). Furthermore, p-PAS-10 was implanted into Beagle calvarial defects and bone regeneration was analyzed after 4 weeks. New bone formation was assessed by calcein and Masson's trichrome staining. The data reveal that p-PAS group exhibits significantly enhanced oseto-regenerative capability in vivo. The peptide-modified PAS with promoted bioactivity and osteogenic differentiation in vitro as well as bone formation ability in vivo could be promising tissue engineering materials for repairing and regeneration of bone defects.
authors.affiliation: Second Dental Center, Central Laboratory, School and Hospital of Stomatology, Peking University, Beijing 100081, China; Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
authors.forename: Zuyuan
authors.initials: Z
authors.lastname: Luo
authors.affiliation: Department of Stomatology, Xuanwu Hospital, Capital Medical University, Beijing 00053, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Second Dental Center, Central Laboratory, School and Hospital of Stomatology, Peking University, Beijing 100081, China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Materials Science and Engineering, College of Engineering, Peking University, Beijing 100871, China.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Yang
authors.affiliation: Second Dental Center, Central Laboratory, School and Hospital of Stomatology, Peking University, Beijing 100081, China; Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China. Electronic address: sc-wei@pku.edu.cn.
authors.forename: Shicheng
authors.initials: S
authors.lastname: Wei
mesh_terms: D000464:Alginates / Q000737:chemistry* / Q000494:pharmacology; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D055419:Bone Morphogenetic Protein 7 / Q000737:chemistry / Q000494:pharmacology; D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology; D001842:Bone and Bones / Q000378:metabolism / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004285:Dogs; D006801:Humans; D008297:Male; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D010012:Osteogenesis; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology; D010455:Peptides / Q000737:chemistry*; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000464:Alginates; D055419:Bone Morphogenetic Protein 7; D010446:Peptide Fragments; D010455:Peptides; C576241:bone forming peptide-1; D000469:Alkaline Phosphatase
keywords: 3D porous scaffolds; Alginate; Bioactivity; Bone tissue engineering; Peptide