title: Temperature-Switchable Control of Ligand Display on Adlayers of Mixed Poly(lysine)-g-(PEO) and Poly(lysine)-g-(ligand-modified poly-N-isopropylacrylamide).
abstract: Adlayers of poly(lysine)-g-PEG comblike copolymer are extensively used to prepare cell-repellant and protein-repellent surfaces by a straightforward coulomb-driven adsorption that is compatible with diverse substrates (glass, Petri dish, etc.). To endow surfaces with functional properties, namely, controlled ligand-protein binding, comblike poly(lysine) derivatives were used to deposit temperature-responsive poly(NIPAM) macrografts mixed with PEG ones on glass surfaces. Simple surface immersion in mixed solutions of biotin-modified poly(lysine)-g-poly(N-isopropylacrylamide) and poly(lysine)-g-poly(ethylene oxide) yielded robust adlayers whose composition reflected the ratio between the two polymers in solution. We show by fluorescence imaging, and comparison with repellent 100% PEGylated patterns, that specific binding of model avidin/particle conjugates (diameters of ca. 10 or 200 nm) was controlled by temperature switch. The biotin ligand was displayed and accessible at low T, or hidden at T > LCST. Topography and mechanical mapping measurements by AFM confirmed the swelling/collapse status of PNIPAM macrografts in the adlayer at low/high T, respectively. Temperature-responsive comblike PLL derivative that can spontaneously cover anionic interfaces is a promising platform enabling good control on the deposition and accessibility of biofunctional groups on various solid surfaces.
authors.affiliation: Ecole Normale Supérieure-PSL Research University, Dpt Chimie, Sorbonne Universités - UPMC Univ. Paris 06, CNRS UMR 8640, 24 rue Lhomond, 75005 Paris, France.
authors.forename: F
authors.initials: F
authors.lastname: Dalier
authors.affiliation: U1006 INSERM, Aix-Marseille Université, Parc Scientifique et Technologique de Luminy, 163 av. de Luminy, 13009 Marseille, France.
authors.forename: F
authors.initials: F
authors.lastname: Eghiaian
authors.affiliation: U1006 INSERM, Aix-Marseille Université, Parc Scientifique et Technologique de Luminy, 163 av. de Luminy, 13009 Marseille, France.
authors.forename: S
authors.initials: S
authors.lastname: Scheuring
authors.affiliation: Ecole Normale Supérieure-PSL Research University, Dpt Chimie, Sorbonne Universités - UPMC Univ. Paris 06, CNRS UMR 8640, 24 rue Lhomond, 75005 Paris, France.
authors.forename: E
authors.initials: E
authors.lastname: Marie
authors.affiliation: Ecole Normale Supérieure-PSL Research University, Dpt Chimie, Sorbonne Universités - UPMC Univ. Paris 06, CNRS UMR 8640, 24 rue Lhomond, 75005 Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Tribet
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D008024:Ligands; D011092:Polyethylene Glycols / Q000737:chemistry*; D011107:Polylysine / Q000031:analogs & derivatives* / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties; D013696:Temperature
chemical_list: D000180:Acrylic Resins; D008024:Ligands; D011108:Polymers; D011107:Polylysine; C052970:poly-N-isopropylacrylamide; D011092:Polyethylene Glycols