title: Further studies to evaluate methods of leucoreduction to prevent alloimmune platelet refractoriness and induce tolerance in a dog platelet transfusion model.
abstract: OBJECTIVE
  Three leucoreduction filters were evaluated - when used alone or combined with centrifuge leucoreduction (C-LR) - to prevent alloimmune platelet refractoriness in a dog platelet transfusion model.
  
  
  METHODS
  Donor platelet-rich plasma (PRP) or buffy coat (BC) platelets were either filter leucoreduced (F-LR) or F-LR/C-LR, (51) Cr radiolabelled and transfused. Weekly transfusions were given for up to 8 weeks or until platelet refractoriness. Recipients who accepted treated transfusions were then given non-leucoreduced (non-LR) platelets to determine whether donor-specific tolerance had been induced.
  
  
  RESULTS
  Acceptance of F-LR PRP transfusions ranged from 29% to 66%. F-LR/C-LR transfusions prepared from PRP were accepted by 92%, from BC by 63% and from pooled PRP by 75% of recipients (p=NS); overall acceptance rate of F-LR/C-LR transfusions was 83%. Tolerance to subsequent non-LR transfusions occurred in 45% of the F-LR-/C-LR-accepting recipients unrelated to DR-B compatibility between donors and recipients (P = 0·18).
  
  
  CONCLUSIONS
  In a dog platelet transfusion model, acceptance of donor platelets required combining F-LR with C-LR as apparently each process removes different immunizing WBCs.
authors.affiliation: Research Institute, Bloodworks Northwest, Seattle, WA, USA.|University of Washington School of Medicine, Seattle, WA, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Slichter
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
authors.forename: K
authors.initials: K
authors.lastname: Abrams
authors.affiliation: Research Institute, Bloodworks Northwest, Seattle, WA, USA.
authors.forename: S Lawrence
authors.initials: SL
authors.lastname: Bailey
authors.affiliation: Research Institute, Bloodworks Northwest, Seattle, WA, USA.
authors.forename: E
authors.initials: E
authors.lastname: Pellham
authors.affiliation: Research Institute, Bloodworks Northwest, Seattle, WA, USA.
authors.forename: I
authors.initials: I
authors.lastname: Gettinger
authors.affiliation: Research Institute, Bloodworks Northwest, Seattle, WA, USA.
authors.forename: T
authors.initials: T
authors.lastname: Christoffel
authors.affiliation: Ascendant Laboratories, Bellevue, WA, USA.|Washington Center for Pain Management, Bellevue, WA, USA.
authors.forename: L
authors.initials: L
authors.lastname: Gaur
authors.affiliation: Etubics Corporation, Seattle, WA, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Latchman
authors.affiliation: Research Institute, Bloodworks Northwest, Seattle, WA, USA.
authors.forename: K
authors.initials: K
authors.lastname: Nelson
authors.affiliation: Research Institute, Bloodworks Northwest, Seattle, WA, USA.
authors.forename: D
authors.initials: D
authors.lastname: Bolgiano
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology; D002498:Centrifugation; D002860:Chromium Radioisotopes / Q000737:chemistry / Q000378:metabolism; D004285:Dogs; D005260:Female; D005374:Filtration; D005434:Flow Cytometry; D006650:Histocompatibility Testing; D007958:Leukocyte Count; D007962:Leukocytes / Q000166:cytology* / Q000276:immunology; D023421:Models, Animal; D017713:Platelet Transfusion; D053657:Platelet-Rich Plasma / Q000166:cytology; D013921:Thrombocytopenia
chemical_list: D000906:Antibodies; D002860:Chromium Radioisotopes
keywords: leucoreduction; platelet alloimmunization; platelet refractoriness; platelet transfusion; tolerance induction