title: Proteome-derived peptide library for the elucidation of the cleavage specificity of HF3, a snake venom metalloproteinase.
abstract: The Proteomic Identification of Cleavage Sites (PICS) approach was employed for profiling the substrate specificity of HF3, a hemorrhagic snake venom metalloproteinase (SVMP) from Bothrops jararaca. A tryptic peptide library from human plasma was subject to HF3 cleavage and amino acid occurrence for P6 to P6' sites was mapped. 71 cleavage sites were detected and revealed a clear preference for leucine at P1' position, followed by hydrophobic residues in P2'. PICS confirmed existing data on prime site specificity of SVMPs.
authors.affiliation: Laboratório Especial de Toxinologia Aplicada, Center of Toxins, Immune-response and Cell Signaling, CeTICS, Instituto Butantan, Av. Vital Brasil 1500, São Paulo, 05503-000, Brazil.|Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Luciana
authors.initials: L
authors.lastname: Bertholim
authors.affiliation: Laboratório Especial de Toxinologia Aplicada, Center of Toxins, Immune-response and Cell Signaling, CeTICS, Instituto Butantan, Av. Vital Brasil 1500, São Paulo, 05503-000, Brazil.|Departamento de Ciência e Tecnologia, Universidade Federal de São Paulo, São José dos Campos, SP, Brazil.
authors.forename: André
authors.initials: A
authors.lastname: Zelanis
authors.affiliation: Laboratório Especial de Toxinologia Aplicada, Center of Toxins, Immune-response and Cell Signaling, CeTICS, Instituto Butantan, Av. Vital Brasil 1500, São Paulo, 05503-000, Brazil.|Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Ana K
authors.initials: AK
authors.lastname: Oliveira
authors.affiliation: Laboratório Especial de Toxinologia Aplicada, Center of Toxins, Immune-response and Cell Signaling, CeTICS, Instituto Butantan, Av. Vital Brasil 1500, São Paulo, 05503-000, Brazil. solange.serrano@butantan.gov.br.
authors.forename: Solange M T
authors.initials: SM
authors.lastname: Serrano
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017837:Bothrops / Q000235:genetics* / Q000378:metabolism; D045726:Metalloproteases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D019151:Peptide Library; D020543:Proteome; D030162:Reptilian Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012910:Snake Venoms / Q000737:chemistry* / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D019151:Peptide Library; D020543:Proteome; D030162:Reptilian Proteins; D012910:Snake Venoms; D045726:Metalloproteases
keywords: HF3; Metalloproteinase; Peptide bond specificity; Proteome derived peptide library; Proteomic identification of protease cleavage sites; Snake venom