title: Vertically Aligned Nitrogen-Doped Carbon Nanotube Carpet Electrodes: Highly Sensitive Interfaces for the Analysis of Serum from Patients with Inflammatory Bowel Disease.
abstract: The number of patients suffering from inflammatory bowel disease (IBD) is increasing worldwide. The development of noninvasive tests that are rapid, sensitive, specific, and simple would allow preventing patient discomfort, delay in diagnosis, and the follow-up of the status of the disease. Herein, we show the interest of vertically aligned nitrogen-doped carbon nanotube (VA-NCNT) electrodes for the required sensitive electrochemical detection of lysozyme in serum, a protein that is up-regulated in IBD. To achieve selective lysozyme detection, biotinylated lysozyme aptamers were covalently immobilized onto the VA-NCNTs. Detection of lysozyme in serum was achieved by measuring the decrease in the peak current of the Fe(CN)6(3-/4-) redox couple by differential pulse voltammetry upon addition of the analyte. We achieved a detection limit as low as 100 fM with a linear range up to 7 pM, in line with the required demands for the determination of lysozyme level in patients suffering from IBD. We attained the sensitive detection of biomarkers in clinical samples of healthy patients and individuals suffering from IBD and compared the results to a classical turbidimetric assay. The results clearly indicate that the newly developed sensor allows for a reliable and efficient analysis of lysozyme in serum.
authors.affiliation: Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR CNRS 8520, Lille1 University , Avenue Poincaré-BP60069, 59652 Villeneuve d'Ascq, France.|Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials (Ministry of Education), Shandong University , Jinan 250061, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Chemical Sciences, Ariel University , Ariel 40700, Israel.
authors.forename: Palaniappan
authors.initials: P
authors.lastname: Subramanian
authors.affiliation: Department of Chemical Sciences, Ariel University , Ariel 40700, Israel.
authors.forename: Alex
authors.initials: A
authors.lastname: Schechter
authors.affiliation: Department of Chemistry and Bar Ilan Institute of Nanotechnology and Advanced Materials (BINA), Bar-Ilan University , Ramat Gan, 52900, Israel.
authors.forename: Eti
authors.initials: E
authors.lastname: Teblum
authors.affiliation: Department of Chemistry and Bar Ilan Institute of Nanotechnology and Advanced Materials (BINA), Bar-Ilan University , Ramat Gan, 52900, Israel.
authors.forename: Reut
authors.initials: R
authors.lastname: Yemini
authors.affiliation: Department of Chemistry and Bar Ilan Institute of Nanotechnology and Advanced Materials (BINA), Bar-Ilan University , Ramat Gan, 52900, Israel.
authors.forename: Gilbert Daniel
authors.initials: GD
authors.lastname: Nessim
authors.affiliation: International Center of Biodynamics , 1B Intrarea Portocalelor, Sector 6, 060101, Bucharest, Romania.
authors.forename: Alina
authors.initials: A
authors.lastname: Vasilescu
authors.affiliation: Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials (Ministry of Education), Shandong University , Jinan 250061, China.
authors.forename: Musen
authors.initials: M
authors.lastname: Li
authors.affiliation: Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR CNRS 8520, Lille1 University , Avenue Poincaré-BP60069, 59652 Villeneuve d'Ascq, France.
authors.forename: Rabah
authors.initials: R
authors.lastname: Boukherroub
authors.affiliation: Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR CNRS 8520, Lille1 University , Avenue Poincaré-BP60069, 59652 Villeneuve d'Ascq, France.
authors.forename: Sabine
authors.initials: S
authors.lastname: Szunerits
mesh_terms: D004563:Electrochemistry; D004566:Electrodes; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000097:blood*; D009113:Muramidase / Q000097:blood; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D009584:Nitrogen / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013499:Surface Properties
chemical_list: D037742:Nanotubes, Carbon; D009113:Muramidase; D009584:Nitrogen
keywords: aptamers; differential pulse voltammetry; inflammatory bowel disease; lysozyme; nitrogen-doped vertically aligned carbon nanotubes; sensing