title: Changes in Relative Thylakoid Protein Abundance Induced by Fluctuating Light in the Diatom Thalassiosira pseudonana.
abstract: One of the hallmarks of marine diatom biology is their ability to cope with rapid changes in light availability due to mixing of the water column and the lens effect. We investigated how irradiance fluctuations influence the relative abundance of key photosynthetic proteins in the centric diatom Thalassiosira pseudonana by means of mass-spectrometry-based approaches for relative protein quantitation. Most notably, fluctuating-light conditions lead to a substantial overall up-regulation of light-harvesting complex proteins as well as several subunits of photosystems II and I. Despite an initial delay in growth under FL, there were no indications of FL-induced photosynthesis limitation, in contrast to other photosynthetic organisms. Our findings further strengthen the notion that diatoms use a qualitatively different mechanism of photosynthetic regulation in which chloroplast-mitochondria interaction has overtaken crucial regulatory processes of photosynthetic light reactions that are typical for the survival of land plants, green algae, and cyanobacteria.
authors.affiliation: Department of Biochemistry, Molecular Plant Biology, University of Turku , Turku, FI-20520, Finland.
authors.forename: Irina
authors.initials: I
authors.lastname: Grouneva
authors.affiliation: Department of Biochemistry, Molecular Plant Biology, University of Turku , Turku, FI-20520, Finland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Muth-Pawlak
authors.affiliation: Department of Biochemistry, Molecular Plant Biology, University of Turku , Turku, FI-20520, Finland.
authors.forename: Natalia
authors.initials: N
authors.lastname: Battchikova
authors.affiliation: Department of Biochemistry, Molecular Plant Biology, University of Turku , Turku, FI-20520, Finland.
authors.forename: Eva-Mari
authors.initials: EM
authors.lastname: Aro
mesh_terms: D060365:Chloroplast Proteins / Q000032:analysis*; D002736:Chloroplasts / Q000378:metabolism; D017377:Diatoms / Q000737:chemistry*; D008027:Light; D045342:Light-Harvesting Protein Complexes / Q000235:genetics; D008928:Mitochondria / Q000378:metabolism; D010788:Photosynthesis; D045331:Photosystem I Protein Complex / Q000235:genetics; D045332:Photosystem II Protein Complex / Q000235:genetics; D020524:Thylakoids / Q000737:chemistry*; D015854:Up-Regulation / Q000528:radiation effects
chemical_list: D060365:Chloroplast Proteins; D045342:Light-Harvesting Protein Complexes; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex
keywords: diatoms; fluctuating light; mass spectrometry; photosynthesis regulation; relative protein quantitation; thylakoid proteome