title: Actin Interacts with Dengue Virus 2 and 4 Envelope Proteins.
abstract: Dengue virus (DENV) remains a significant public health problem in many tropical and sub-tropical countries worldwide. The DENV envelope (E) protein is the major antigenic determinant and the protein that mediates receptor binding and endosomal fusion. In contrast to some other DENV proteins, relatively few cellular interacting proteins have been identified. To address this issue a co-immuoprecipitation strategy was employed. The predominant co-immunoprecipitating proteins identified were actin and actin related proteins, however the results suggested that actin was the only bona fide interacting partner. Actin was shown to interact with the E protein of DENV 2 and 4, and the interaction between actin and DENV E protein was shown to occur in a truncated DENV consisting of only domains I and II. Actin was shown to decrease during infection, but this was not associated with a decrease in gene transcription. Actin-related proteins also showed a decrease in expression during infection that was not transcriptionally regulated. Cytoskeletal reorganization was not observed during infection, suggesting that the interaction between actin and E protein has a cell type specific component.
authors.affiliation: Institute of Molecular Biosciences, Mahidol University, Salaya campus, 25/25 Phuttamonton Sai 4, Salaya, Nakorn Pathom, Thailand.
authors.forename: Kunlakanya
authors.initials: K
authors.lastname: Jitoboam
authors.affiliation: National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani, Thailand.
authors.forename: Narumon
authors.initials: N
authors.lastname: Phaonakrop
authors.affiliation: Institute of Molecular Biosciences, Mahidol University, Salaya campus, 25/25 Phuttamonton Sai 4, Salaya, Nakorn Pathom, Thailand.
authors.forename: Sirikwan
authors.initials: S
authors.lastname: Libsittikul
authors.affiliation: Institute of Molecular Biosciences, Mahidol University, Salaya campus, 25/25 Phuttamonton Sai 4, Salaya, Nakorn Pathom, Thailand.
authors.forename: Chutima
authors.initials: C
authors.lastname: Thepparit
authors.affiliation: National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani, Thailand.
authors.forename: Sittiruk
authors.initials: S
authors.lastname: Roytrakul
authors.affiliation: Institute of Molecular Biosciences, Mahidol University, Salaya campus, 25/25 Phuttamonton Sai 4, Salaya, Nakorn Pathom, Thailand.|Center for Emerging and Neglected Infectious Diseases, Mahidol University, Salaya campus, 25/25 Phuttamonton Sai 4, Salaya, Nakorn Pathom, Thailand.
authors.forename: Duncan R
authors.initials: DR
authors.lastname: Smith
mesh_terms: D000199:Actins / Q000032:analysis / Q000378:metabolism*; D000330:Aedes; D000818:Animals; D000914:Antibodies, Viral; D002460:Cell Line; D003715:Dengue / Q000378:metabolism*; D003716:Dengue Virus / Q000502:physiology*; D057809:HEK293 Cells; D055551:HSP27 Heat-Shock Proteins / Q000032:analysis / Q000378:metabolism; D054884:Host-Pathogen Interactions; D006801:Humans; D009218:Myosins / Q000032:analysis / Q000378:metabolism; D060066:Protein Interaction Maps; D014759:Viral Envelope Proteins / Q000032:analysis / Q000378:metabolism*
chemical_list: D000199:Actins; D000914:Antibodies, Viral; D055551:HSP27 Heat-Shock Proteins; D014759:Viral Envelope Proteins; D009218:Myosins