title: Quantification of ethanol in plasma by electrochemical detection with an unmodified screen printed carbon electrode.
abstract: Simple, rapid and accurate detection of ethanol concentration in blood is very crucial in the diagnosis and management of potential acute ethanol intoxication patients. A novel electrochemical detection method was developed for the quantification of ethanol in human plasma with disposable unmodified screen-printed carbon electrode (SPCE) without sample preparation procedure. Ethanol was detected indirectly by the reaction product of ethanol dehydrogenase (ADH) and cofactor nicotinamide adenine dinucleotide (NAD(+)). Method validation indicated good quantitation precisions with intra-day and inter-day relative standard deviations of ≤9.4% and 8.0%, respectively. Ethanol concentration in plasma is linear ranging from 0.10 to 3.20 mg/mL, and the detection limit is 40.0 μg/mL (S/N > 3). The method shows satisfactory correlation with the reference method of headspace gas chromatography in twenty human plasma samples (correlation coefficient 0.9311). The proposed method could be applied to diagnose acute ethanol toxicity or ethanol-related death.
authors.affiliation: Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education of China), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, P. R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Tian
authors.affiliation: Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education of China), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, P. R. China.
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education of China), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, P. R. China.
authors.forename: Ming-Song
authors.initials: MS
authors.lastname: Zhu
authors.affiliation: Center of Evidence Identification, Chongqing Police Bureau, Chongqing, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education of China), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, P. R. China.
authors.forename: Zheng-Hu
authors.initials: ZH
authors.lastname: Shi
authors.affiliation: Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education of China), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, P. R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Ding
mesh_terms: D000426:Alcohol Dehydrogenase / Q000378:metabolism; D002244:Carbon / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D000431:Ethanol / Q000032:analysis* / Q000097:blood; D006801:Humans; D057230:Limit of Detection; D009243:NAD / Q000378:metabolism; D010949:Plasma / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D009243:NAD; D000431:Ethanol; D002244:Carbon; D000426:Alcohol Dehydrogenase