title: [EFFECT OF HYPOXIA ON SYNAPTIC TRANSMISSION BETWEEN RETINAL GANGLION CELLS AND SUPERIOR COLLICULUS NEURONS IN COCULTURE].
abstract: In this study we conducted a series of experiments to characterize the effect and define the mechanisms of hypoxia on synaptic transmission between retinal ganglion cells and superior colliculus (SC) neurons. Application of hypoxic solution leads to a long lasting potentiation (LTP) NMDA-mediated synaptic transmission. Analysis of the oxygen deficiency effect on the spontaneous and miniature postsynaptic currents (sPSC and mPSC respectively) revealed an increase in the frequency of their occurrence and the appearance of the second peak in the mPSC histogram distribution. The assessment of quantum and binomial parameters reflects the complex pre- and postsynaptic changes during the potentiation, independent of the release probability. Most likely this LTP can be caused by an increase in the total number of active synapses. Glutamatergic synaptic transmission mediated by non-NMDA activation receptor-channel complexes, responded to application of deoxygenated solution by the brief depression, which is the result of presynaptic dysfunction and associates with decrease in release probability and number of active zones. GABAergic synaptic transmission mediated by activation GABA(A)-receptor-channel complexes, responded to hypoxic action by long term depression (LTD). Analysis of sPSC and mPSC showed a significant decrease in the frequency of their occurrence and significant (P = 0.05) decrease in the quantum over a period of oxygen deficiency. In general, the effect of hypoxia-induced LTD of GABAergic synaptic transmission is based on complex changes of presynaptic (independent on the release probability) and postsynaptic (reduction sensitivity of receptors in postsynaptic membrane) mechanisms.
authors.forename: G V
authors.initials: GV
authors.lastname: Dumanska
authors.forename: O V
authors.initials: OV
authors.lastname: Rikhalsky
authors.forename: N S
authors.initials: NS
authors.lastname: Veselovsky
mesh_terms: D000818:Animals; D015687:Cell Hypoxia; D018920:Coculture Techniques; D015870:Gene Expression; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology; D036881:Long-Term Synaptic Depression / Q000187:drug effects / Q000502:physiology; D055369:Miniature Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D010100:Oxygen / Q000494:pharmacology*; D051381:Rats; D011963:Receptors, GABA-A / Q000235:genetics / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics / Q000378:metabolism; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D013477:Superior Colliculi / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D013569:Synapses / Q000187:drug effects / Q000502:physiology; D009435:Synaptic Transmission / Q000187:drug effects* / Q000502:physiology
chemical_list: D011963:Receptors, GABA-A; D016194:Receptors, N-Methyl-D-Aspartate; D010100:Oxygen