title: Identification and RNA segment assignment of six structural proteins of Scylla serrata reovirus.
abstract: Scylla serrata reovirus (SsRV) is one of the most prevalent viral pathogens of the mud crab (S. serrata). The virus represents an unassigned novel genus in the Reoviridae family, and contains 12 double-stranded RNA genomic segments. Previous analysis of virion proteins concluded that SsRV contains at least eight structural proteins, ranging from 25 to 160 kDa. Here, tandem time-of-flight mass spectrometry and Western blotting were used to re-identify the structural proteins. The results indicate that proteins encoded by SsRV segments S1, S3, S6, S9, S11, and S12 are structural proteins.
authors.affiliation: College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai, People's Republic of China.|College of Biological and Environmental Sciences, Zhejiang Wanli University, No. 8, South Qianhu Road, Ningbo, 315100, Zhejiang Province, People's Republic of China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: College of Biological and Environmental Sciences, Zhejiang Wanli University, No. 8, South Qianhu Road, Ningbo, 315100, Zhejiang Province, People's Republic of China.
authors.forename: Dongyang
authors.initials: D
authors.lastname: Fan
authors.affiliation: College of Biological and Environmental Sciences, Zhejiang Wanli University, No. 8, South Qianhu Road, Ningbo, 315100, Zhejiang Province, People's Republic of China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Biological and Environmental Sciences, Zhejiang Wanli University, No. 8, South Qianhu Road, Ningbo, 315100, Zhejiang Province, People's Republic of China.
authors.forename: Jifang
authors.initials: J
authors.lastname: Yang
authors.affiliation: Bioengineering College of Jimei University, Xiamen, People's Republic of China.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Biological and Environmental Sciences, Zhejiang Wanli University, No. 8, South Qianhu Road, Ningbo, 315100, Zhejiang Province, People's Republic of China. genomic@163.com.
authors.forename: Jigang
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D003386:Brachyura / Q000821:virology*; D012330:RNA, Double-Stranded / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D012087:Reoviridae / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*; D014771:Virion / Q000235:genetics
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral; D015678:Viral Structural Proteins
keywords: Mass spectrometry; Reovirus; Scylla serrata; Structural proteins