title: Screening and characterizing a denitrifying phosphorus-accumulating bacterium isolated from a circular plug-flow reactor.
abstract: Denitrifying phosphorus-accumulating organisms (DNPAO) are viewed as one of the most effective means to solve the removal contradiction of nitrogen and phosphorus in wastewater treatment. In this study, we isolated a DNPAO (C-17, accession number: KU745702) from activated sludge in a patented circular plug-flow reactor, physiologically to Pseudomonas sp. based on 16S rRNA sequence and phenotypic characteristics. The results of denitrifying phosphorus-accumulating experiment showed that Pseudomonas C-17 has high removal efficiencies for [Formula: see text] and NO3-N, 75% and 87%, respectively. The ratio of phosphorus release was 25.0 mg [Formula: see text] (with anabolism) and 26.8 mg [Formula: see text] (without anabolism), respectively. Our results indicated that Pseudomonas C-17 had strong capacity of phosphorus release, and its uptake is often imprecisely evaluated by ignoring the part of metabolic consumption. Pseudomonas C-17 is capable of utilizing oxygen, nitrate and nitrite as electron acceptors under experimental conditions.
authors.affiliation: a College of Water Sciences , Beijing Normal University , Beijing , People's Republic of China.
authors.forename: En
authors.initials: E
authors.lastname: Xie
authors.affiliation: a College of Water Sciences , Beijing Normal University , Beijing , People's Republic of China.
authors.forename: Aizhong
authors.initials: A
authors.lastname: Ding
authors.affiliation: a College of Water Sciences , Beijing Normal University , Beijing , People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zheng
authors.affiliation: a College of Water Sciences , Beijing Normal University , Beijing , People's Republic of China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Dou
authors.affiliation: b Department of Civil Engineering , Queen's University , Kingston , ON , Canada.
authors.forename: Bruce
authors.initials: B
authors.lastname: Anderson
authors.affiliation: a College of Water Sciences , Beijing Normal University , Beijing , People's Republic of China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Huang
authors.affiliation: a College of Water Sciences , Beijing Normal University , Beijing , People's Republic of China.
authors.forename: Ruoting
authors.initials: R
authors.lastname: Jing
mesh_terms: D019149:Bioreactors; D058440:Denitrification; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism; D011549:Pseudomonas / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D009566:Nitrates; D009573:Nitrites; D010710:Phosphates; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; D010758:Phosphorus
keywords: 16S rRNA; Pseudomonas sp; denitrification; phosphorus; poly-β-hydroxybutyrate