title: Potency of a thermostabilised chimpanzee adenovirus Rift Valley Fever vaccine in cattle.
abstract: Development of safe and efficacious vaccines whose potency is unaffected by long-term storage at ambient temperature would obviate major vaccine deployment hurdles and limit wastage associated with breaks in the vaccine cold chain. Here, we evaluated the immunogenicity of a novel chimpanzee adenovirus vectored Rift Valley Fever vaccine (ChAdOx1-GnGc) in cattle, following its thermostabilisation by slow desiccation on glass fiber membranes in the non-reducing sugars trehalose and sucrose. Thermostabilised ChAdOx1-GnGc vaccine stored for 6 months at 25, 37 or 45 ° C elicited comparable Rift Valley Fever virus neutralising antibody titres to those elicited by the 'cold chain' vaccine (stored at -80 ° C throughout) at the same dose, and these were within the range associated with protection against Rift Valley Fever in cattle. The results support the use of sugar-membrane thermostabilised vaccines in target livestock species.
authors.affiliation: The Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford OX3 7DQ, UK.
authors.forename: Pawan
authors.initials: P
authors.lastname: Dulal
authors.affiliation: The Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford OX3 7DQ, UK.
authors.forename: Daniel
authors.initials: D
authors.lastname: Wright
authors.affiliation: The Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford OX3 7DQ, UK.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Ashfield
authors.affiliation: The Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford OX3 7DQ, UK.
authors.forename: Adrian V S
authors.initials: AV
authors.lastname: Hill
authors.affiliation: The Pirbright Institute, Ash Road, Pirbright, Woking GU24 0NF, UK.
authors.forename: Bryan
authors.initials: B
authors.lastname: Charleston
authors.affiliation: The Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford OX3 7DQ, UK; Centre for Research on Therapeutic Sciences and, Institute for Healthcare Management Strathmore University, P.O. Box 59857-00200, Nairobi, Kenya. Electronic address: george.warimwe@ndm.ox.ac.uk.
authors.forename: George M
authors.initials: GM
authors.lastname: Warimwe
mesh_terms: D000256:Adenoviridae; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D004355:Drug Stability; D002679:Pan troglodytes / Q000821:virology; D012034:Refrigeration; D012295:Rift Valley Fever / Q000517:prevention & control*; D013395:Sucrose / Q000737:chemistry; D013696:Temperature; D014199:Trehalose / Q000737:chemistry; D014765:Viral Vaccines / Q000737:chemistry / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D014765:Viral Vaccines; D013395:Sucrose; D014199:Trehalose
keywords: Adenovirus vaccine; Rift Valley Fever; Thermostable