title: Expression of prostaglandin receptors in Chlamydia trachomatis-infected recurrent spontaneous aborters.
abstract: A study was undertaken to quantify the expression of prostaglandin (PG) receptors and find the effect of gestational age on expression of PG receptor genes in Chlamydia trachomatis-infected recurrent spontaneous aborters (RSA). Endometrial curettage tissue (ECT) was collected from 130 RSA (Group I) and 100 age-matched controls (Group II) at the Department of Obstetrics and Gynecology, Safdarjung Hospital, New Delhi (India). PCR was performed for diagnosis of C. trachomatis cryptic plasmid; mRNA expression of PG receptor genes was assessed by real-time PCR (q-PCR), while serum progesterone/estrogen levels were determined by respective commercial kits. Data were evaluated statistically. A total of 15.4 % RSA (GroupI) were diagnosed as C. trachomatis-positive (200 bp), whereas controls were uninfected. q-PCR showed significant upregulation (P<0.0001) of PGE2 (EP-1, EP-2, EP-3, EP-4), PGF2α (FP) and PGI2 (IP) receptors in Group I versus Group II. The expression of PG receptors increased significantly with advanced gestational age (P<0.002); however, only contractile receptors, EP-1, EP-3 and FP, were positively correlated with gestational age in Group-I. In infected RSA, mean serum progesterone level was significantly low (P<0.0001) while serum oestrogen was high (P<0.0001). Overall, the data suggest that increased expression of PG receptors, particularly contractile gene receptors (EP-1, EP-3, FP), with advanced gestational age and altered steroid levels could be a possible risk factor for abortion in Chlamydia-infected RSA.
authors.affiliation: Microbiology Laboratory, National Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi-110 029, India.
authors.forename: Namita
authors.initials: N
authors.lastname: Singh
authors.affiliation: Microbiology Laboratory, National Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi-110 029, India.
authors.forename: Priya
authors.initials: P
authors.lastname: Prasad
authors.affiliation: Central Instrumentation Facility, National Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi-110 029, India.
authors.forename: Laishram Chandreshwar
authors.initials: LC
authors.lastname: Singh
authors.affiliation: Department of Obstetrics & Gynecology, Vardhaman Mahavir Medical College (VMMC) & Safdarjung Hospital, New Delhi-110 029, India.
authors.forename: Banashree
authors.initials: B
authors.lastname: Das
authors.affiliation: Microbiology Laboratory, National Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi-110 029, India.
authors.forename: Sangita
authors.initials: S
authors.lastname: Rastogi
mesh_terms: D000026:Abortion, Habitual / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D002690:Chlamydia Infections / Q000150:complications*; D002692:Chlamydia trachomatis; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology*; D011982:Receptors, Prostaglandin / Q000235:genetics / Q000378:metabolism*
chemical_list: D011982:Receptors, Prostaglandin