title: Expression of p53 protein, Jaagsiekte sheep retrovirus matrix protein, and surfactant protein in the lungs of sheep with pulmonary adenomatosis.
abstract: Ovine pulmonary adenocarcinoma (OPA) is a naturally occurring cancer in sheep that is caused by the Jaagsiekte sheep retrovirus (JSRV). Because the pathologic and epidemiologic features of OPA are similar to those of bronchoalveolar carcinoma in humans, OPA is considered a useful animal model for pulmonary carcinogenesis. In this study, 3,512 lungs from various breeds of sheep were collected and macroscopically examined. OPA was identified in 30 sheep, and samples of these animals were further examined by histologic, immunohistochemical (p53 protein, surfactant protein A [SP-A], proliferating cell nuclear antigen [PCNA], JSRV matrix protein [MA]), and PCR methods. Papillary or acinar adenocarcinomas were detected microscopically in the affected areas. Immunoreactivity for p53 PAb240 was detected in 13 sheep, whereas p53 DO-1 was not detected in any of the OPA animals. PCNA immunoreactivity was recorded in 27 animals. SP-A and JSRV MA protein was immunopositive in all 30. JSRV proviral DNA was detected by PCR analysis in all of the lung samples collected from OPA animals. In addition, the pulmonary SP-A levels were increased in tumor cells. The results of this study suggest that PCNA and p53 protein expression may be useful indicators in monitoring malignancy of pulmonary tumors.
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, Yuzuncu Yıl University, Van, Turkey (İlhan, Yıldırım)Department of Pathology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey (Vural, Alcigir)Department of Virology, Faculty of Veterinary Medicine, Erciyes University, Kayseri, Turkey (Sözdutmaz) fatmasayn@yahoo.com.
authors.forename: Fatma
authors.initials: F
authors.lastname: İlhan
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, Yuzuncu Yıl University, Van, Turkey (İlhan, Yıldırım)Department of Pathology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey (Vural, Alcigir)Department of Virology, Faculty of Veterinary Medicine, Erciyes University, Kayseri, Turkey (Sözdutmaz).
authors.forename: Sevil A
authors.initials: SA
authors.lastname: Vural
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, Yuzuncu Yıl University, Van, Turkey (İlhan, Yıldırım)Department of Pathology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey (Vural, Alcigir)Department of Virology, Faculty of Veterinary Medicine, Erciyes University, Kayseri, Turkey (Sözdutmaz).
authors.forename: Serkan
authors.initials: S
authors.lastname: Yıldırım
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, Yuzuncu Yıl University, Van, Turkey (İlhan, Yıldırım)Department of Pathology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey (Vural, Alcigir)Department of Virology, Faculty of Veterinary Medicine, Erciyes University, Kayseri, Turkey (Sözdutmaz).
authors.forename: İbrahim
authors.initials: İ
authors.lastname: Sözdutmaz
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, Yuzuncu Yıl University, Van, Turkey (İlhan, Yıldırım)Department of Pathology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey (Vural, Alcigir)Department of Virology, Faculty of Veterinary Medicine, Erciyes University, Kayseri, Turkey (Sözdutmaz).
authors.forename: Mehmet E
authors.initials: ME
authors.lastname: Alcigir
mesh_terms: D000818:Animals; D007150:Immunohistochemistry / Q000662:veterinary; D022141:Jaagsiekte sheep retrovirus / Q000472:pathogenicity; D008168:Lung / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011648:Pulmonary Adenomatosis, Ovine / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D037662:Pulmonary Surfactant-Associated Protein A / Q000378:metabolism; D012756:Sheep; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D037662:Pulmonary Surfactant-Associated Protein A; D016159:Tumor Suppressor Protein p53
keywords: Jaagsiekte sheep retrovirus; PCR; matrix protein; p53; sheep pulmonary adenomatosis; surfactant protein