title: Molecular insight of DREAM and presenilin 1 C-terminal fragment interactions.
abstract: Interactions between downstream regulatory element antagonist modulator (DREAM) and presenilin 1 (PS1) are related to numerous neuronal processes. We demonstrate that association of PS1 carboxyl peptide (residues 445-467, HL9) with DREAM is calcium dependent and stabilized by a cluster of three aromatic residues: F462 and F465 from PS1 and F252 from DREAM. Additional stabilization is provided by residues in a loop connecting α helices 7 and 8 in DREAM and residues of PS1, namely cation-π interactions between R200 in DREAM and F465 in PS1 and the salt bridges formed by R207 in DREAM and D450 and D458 in PS1.
authors.affiliation: Department of Chemistry and Biochemistry, Florida International University, Miami, FL, USA.
authors.forename: Khoa
authors.initials: K
authors.lastname: Pham
authors.affiliation: Department of Chemistry and Biochemistry, Florida International University, Miami, FL, USA.|Biomolecular Sciences Institute, Florida International University, Miami, FL, USA.
authors.forename: Jaroslava
authors.initials: J
authors.lastname: Miksovska
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D016880:Anisotropy; D002118:Calcium / Q000378:metabolism; D020832:EF Hand Motifs; D006801:Humans; D007700:Kinetics; D051605:Kv Channel-Interacting Proteins / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D053764:Presenilin-1 / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D000072417:Protein Domains; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D017186:Titrimetry
chemical_list: D000596:Amino Acids; D051605:Kv Channel-Interacting Proteins; D010455:Peptides; D053764:Presenilin-1; D002118:Calcium
keywords: Alzheimer's disease; DREAM; calsenilin; fluorescence anisotropy; potassium channel interacting protein; presenilin-binding interfaces