title: Are the optimum levels of the catalase and vitamin E in rooster semen extender after freezing-thawing influenced by sperm concentration?
abstract: To date, there has no report to evaluate the interaction effects of antioxidant and sperm concentration in rooster semen cryopreservation. This study was aimed to investigate the effects of vitamin E (VitE) and catalase (CAT) at different sperm concentrations on the rooster post-thawed sperm quality. Semen samples were collected twice a week from ten roosters (ROS 308) and diluted according to experimental treatments. The treatments consist of different sperm concentrations (200, 400 and 600 × 106 sperm/mL) with supplementation VitE (5 μg/mL; VitE200, VitE400, and VitE600, respectively) or CAT (100 IU/mL; CAT200, CAT400, CAT600, respectively) and without antioxidants [Control (Con); Con200, Con400, Con600, respectively]. After thawing, motion characteristics were assessed using a CASA system. Plasma membrane integrity and malondialdehyde (MDA) level were evaluated with Hypoosmotic swelling test (HOST) and Thiobarbituric acid (TBA), respectively. The higher percentage of total motility, progressive motility, viability and membrane integrity were obtained in VitE400 (81.16 ± 1.21, 18.44 ± 1.19, 85.47 ± 1.07, 86.91 ± 1.16, respectively) and CAT400 (79.38 ± 1.21, 17.19 ± 1.19, 83.42 ± 1.07, 85.73 ± 1.16, respectively) compared to control groups. Moreover, the lowest percentage of MDA was measured in VitE400, VitE600 and CAT400 rather than other groups (1.489, 1.500, 1.510 ± 0.06, respectively). In conclusion, the results of the present study demonstrate that VitE (5 μg/mL) and CAT (100 IU/mL) independently at sperm concentration, 400 million sperm/mL could beneficial effect for preservation of rooster semen during cryopreservation.
authors.affiliation: Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
authors.forename: Morteza
authors.initials: M
authors.lastname: Moghbeli
authors.affiliation: Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran; Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran. Electronic address: hamid_kohram@yahoo.com.
authors.forename: Hamid
authors.initials: H
authors.lastname: Kohram
authors.affiliation: Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran. Electronic address: azareh@ut.ac.ir.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Zare-Shahaneh
authors.affiliation: Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Zhandi
authors.affiliation: Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Sharafi
authors.affiliation: Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Nabi
authors.affiliation: Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Zahedi
authors.affiliation: Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Sharideh
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D002645:Chickens; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D005615:Freezing; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D012661:Semen; D012662:Semen Preservation / Q000379:methods*; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D003451:Cryoprotective Agents; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D008315:Malondialdehyde; D002374:Catalase
keywords: Antioxidant; Apoptotic sperm; Mitochondria activity; Reactive oxygen species; Semen concentration