title: Protective effects of honokiol on ischemia/reperfusion injury of rat ovary: an experimental study.
abstract: OBJECTIVE
  The purpose of this study was to investigate the protective effect of honokiol on experimental ischemia/reperfusion injury of rat ovary.
  
  
  METHODS
  A total of 40 female Wistar albino rats were used in this study. The rats were divided into five groups as follows: sham (Group I), torsion (Group II), torsion + detorsion (Group III), torsion + detorsion + saline (Group IV), and torsion + detorsion + honokiol (Group V). Bilateral adnexa in all the rats except for those in the sham group were exposed to torsion for 3 hours. The rats in Group IV were administered saline, whereas the rats in Group V were administered honokiol by intraperitoneal route 30 minutes before detorsion. Tissue and plasma concentrations of malondialdehyde and nitric oxide were determined. Ovarian tissue was histologically evaluated. Data analyses were performed by means of Kruskal-Wallis test and Mann-Whitney U-test (Bonferroni correction) in SPSS 15.0 (Statistical Package for Social Sciences; SPSS Inc., Chicago, IL, USA).
  
  
  RESULTS
  The torsion and detorsion groups had higher scores in vascular congestion, hemorrhage, and inflammatory cell infiltration compared with the sham group (P<0.005). In addition, total histopathological scores were significantly higher in the torsion and detorsion groups compared with the sham group (P<0.005). A significant reduction was observed in hemorrhage, inflammatory cell infiltration, and cellular degeneration scores, of all histopathological scores, in the honokiol group (P<0.005). Ovarian tissue concentrations of malondialdehyde were significantly higher in the torsion and detorsion groups compared with the sham and honokiol groups (P<0.005). Ovarian tissue concentrations of nitric oxide, on the other hand, were significantly higher in the torsion group compared with the sham, saline, and honokiol groups (P<0.005).
  
  
  CONCLUSIONS
  Honokiol has a beneficial effect on ovarian torsion-related ischemia/reperfusion injury.
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Dicle University, Diyarbakir, Turkey.
authors.forename: Senem
authors.initials: S
authors.lastname: Yaman Tunc
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Dicle University, Diyarbakir, Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Agacayak
authors.affiliation: Department of Obstetrics and Gynecology, Diyarbakir Memorial Hospital, Dicle University, Diyarbakir, Turkey.
authors.forename: Neval Yaman
authors.initials: NY
authors.lastname: Goruk
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Dicle University, Diyarbakir, Turkey.
authors.forename: Mehmet Sait
authors.initials: MS
authors.lastname: Icen
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Dicle University, Diyarbakir, Turkey.
authors.forename: Abdulkadir
authors.initials: A
authors.lastname: Turgut
authors.affiliation: Department of Pathology, School of Medicine, Dicle University, Diyarbakir, Turkey.
authors.forename: Ulas
authors.initials: U
authors.lastname: Alabalik
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Hitit University, Corum, Turkey.
authors.forename: Cihan
authors.initials: C
authors.lastname: Togrul
authors.affiliation: Department of Histology and Embryology, Dicle University, Diyarbakir, Turkey.
authors.forename: Cenap
authors.initials: C
authors.lastname: Ekinci
authors.affiliation: Department of Medical Biochemistry, School of Medicine, Dicle University, Diyarbakir, Turkey.
authors.forename: Aysun
authors.initials: A
authors.lastname: Ekinci
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Dicle University, Diyarbakir, Turkey.
authors.forename: Talip
authors.initials: T
authors.lastname: Gul
mesh_terms: D000818:Animals; D001713:Biphenyl Compounds / Q000627:therapeutic use*; D005260:Female; D017705:Lignans / Q000627:therapeutic use*; D010049:Ovarian Diseases / Q000188:drug therapy* / Q000473:pathology; D065840:Protective Factors; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000473:pathology; D000498:Allyl Compounds; D010636:Phenols
chemical_list: D001713:Biphenyl Compounds; D017705:Lignans; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols
keywords: honokiol; ischemia/reperfusion injury; malondialdehyde; nitric oxide; ovary