title: miR-31 is distinctively overexpressed in primary male extramammary Paget's disease.
abstract: MicroRNAs (miRNAs) are small noncoding RNAs involved in cancer development. Extramammary Paget's disease (EMPD) is a rare cutaneous malignancy and the role of miRNAs in EMPD remains unknown. Here, we used TaqMan miRNA arrays to characterize miRNA expression profile in EMPD and further validated the candidates by single RT-PCR. Total 12 cases EMPD were involved in this study. Using laser capture micro-dissection technique, we collected EMPD tumor cells (ET, n=12), normal epidermal cells (NE, n=12) and normal apocrine glands cells (NA, n=7). MiRNA arrays from two pairs of ET and corresponding NE showed that miR-375, miR-10b, miR-31, miR-31* were differentially expressed. The single real-time PCR (RT-PCR) further confirmed that miR-375, miR-31 and miR-31* were upregulated in EMPD cells than those of the normal epidermis and apocrine glands. Our preliminary study suggested that these miRNAs could be involved in EMPD development and miR-31 may serve as potential biomarkers of EMPD.
authors.affiliation: Department of Dermatology, No.1 Hospital of China Medical University, Shenyang, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Guo
authors.affiliation: Department of Dermatology, No.1 Hospital of China Medical University, Shenyang, China.
authors.forename: Rui-Qun
authors.initials: RQ
authors.lastname: Qi
authors.affiliation: Department of Dermatology, No.1 Hospital of China Medical University, Shenyang, China.
authors.forename: Ya-Ni
authors.initials: YN
authors.lastname: Lv
authors.affiliation: Department of Dermatology, No.1 Hospital of China Medical University, Shenyang, China.
authors.forename: He-Xiao
authors.initials: HX
authors.lastname: Wang
authors.affiliation: Department of Dermatology, No.1 Hospital of China Medical University, Shenyang, China.
authors.forename: Yu-Xiao
authors.initials: YX
authors.lastname: Hong
authors.affiliation: Department of Dermatology, No.1 Hospital of China Medical University, Shenyang, China.
authors.forename: Song
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Department of Dermatology, No.1 Hospital of China Medical University, Shenyang, China.
authors.forename: Jiu-Hong
authors.initials: JH
authors.lastname: Li
authors.affiliation: Department of Dermatology, No.1 Hospital of China Medical University, Shenyang, China.
authors.forename: Xing-Hua
authors.initials: XH
authors.lastname: Gao
authors.affiliation: Department of Dermatology, No.1 Hospital of China Medical University, Shenyang, China.
authors.forename: Hong-Duo
authors.initials: HD
authors.lastname: Chen
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D010145:Paget Disease, Extramammary / Q000235:genetics* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; C547183:MIRN10 microRNA, human; C534612:MIRN31 microRNA, human; C540933:MIRN375 microRNA, human; D035683:MicroRNAs
keywords: apocrine glands; extramammary Paget’s disease; miR-31; miRNA; non-melanoma skin cancer