title: Positive Effects of Diphlorethohydroxycarmalol (DPHC) on the Stability of the Integument Structure in Diet-Induced Obese Female Mice.
abstract: Diphlorethohydroxycarmalol (DPHC) is a known to modulate the expression of extracellular matrix (ECM) components in 3T3-L1. However, the possible role of DPHC in integument stability during obesity induction is not clear yet. We evaluated the effects of DPHC on collagen or elastic fiber quantity in integument during obesity induction with high-fat diet. The dorsal back integument sections were stained with hematoxylin-eosin, Masson trichrome, and Verhoff-Van Gieson. The intensities of collagen fibers and elastin fibers were analyzed with ImageJ. The number of fibroblasts was counted at ×1,000 fields. The number of fibroblast was increased by obesity induction, but DPHC suppressed it in a concentrationdependent manner both in lean and obese mice. On the other hand, the intensities of collagen fibers were increased by DPHC treatment in obese mice groups but not in lean mice groups. The intensities of collagen fibers of obese mice were lower than that of the lean mice in 0% group. However, the number became similar between lean and obese mice by the treatment of DPHC. The intensity of elastic fibers was increased in the lean mice with the concentration of DPHC. In the obese mice group, there were increasing patterns but only significant at 10% DPHC group. The intensity of elastic fibers of obese mice was higher than lean mice in 0%, 1%, and 10% groups. Histologically epithelial cells and follicle cells which were diffused nuclear staining forms were increased by DPHC treatment. The results suggest that the activity of integument cells during obesity induction can be modulated by DPHC.
authors.affiliation: Division of Developmental Biology and Physiology, School of Biosciences and Chemistry, Institute for Basic Sciences, Sungshin Women's University, Seoul 142-742, Korea.
authors.forename: Chae-Lim
authors.initials: CL
authors.lastname: Kim
authors.affiliation: Division of Developmental Biology and Physiology, School of Biosciences and Chemistry, Institute for Basic Sciences, Sungshin Women's University, Seoul 142-742, Korea.
authors.forename: Sun-Yeong
authors.initials: SY
authors.lastname: Cha
authors.affiliation: Global Medical Science, Sungshin Women's University, Seoul 142-742, Korea.
authors.forename: Min Young
authors.initials: MY
authors.lastname: Chun
authors.affiliation: Exercise Rehabilitation and Welfare, Sungshin Women's University, Seoul 142-742, Korea.
authors.forename: Bumsoo
authors.initials: B
authors.lastname: Kim
authors.affiliation: Industrial Design, Sungshin Women's University, Seoul 142-742, Korea.
authors.forename: Min Young
authors.initials: MY
authors.lastname: Choi
authors.affiliation: Division of Developmental Biology and Physiology, School of Biosciences and Chemistry, Institute for Basic Sciences, Sungshin Women's University, Seoul 142-742, Korea.
authors.forename: Yong-Pil
authors.initials: YP
authors.lastname: Cheon
keywords: Collagen fibers; Diphlorethyohydroxycarmalol; Elastic fibers; Integument; Obesity induction