title: Imaging analysis of nuclear antiviral factors through direct detection of incoming adenovirus genome complexes.
abstract: Recent studies involving several viral systems have highlighted the importance of cellular intrinsic defense mechanisms through nuclear antiviral proteins that restrict viral propagation. These factors include among others components of PML nuclear bodies, the nuclear DNA sensor IFI16, and a potential restriction factor PHF13/SPOC1. For several nuclear replicating DNA viruses, it was shown that these factors sense and target viral genomes immediately upon nuclear import. In contrast to the anticipated view, we recently found that incoming adenoviral genomes are not targeted by PML nuclear bodies. Here we further explored cellular responses against adenoviral infection by focusing on specific conditions as well as additional nuclear antiviral factors. In line with our previous findings, we show that neither interferon treatment nor the use of specific isoforms of PML nuclear body components results in co-localization between incoming adenoviral genomes and the subnuclear domains. Furthermore, our imaging analyses indicated that neither IFI16 nor PHF13/SPOC1 are likely to target incoming adenoviral genomes. Thus our findings suggest that incoming adenoviral genomes may be able to escape from a large repertoire of nuclear antiviral mechanisms, providing a rationale for the efficient initiation of lytic replication cycle.
authors.affiliation: Microbiologie Fondamentale et Pathogénicité, MFP CNRS UMR 5234, Université de Bordeaux, Bordeaux 33076, France; Department of Infection Biology, Faculty of Medicine, University of Tsukuba, Tsukuba 305-8575, Japan.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Komatsu
authors.affiliation: Department of Tumor Biology, University Hospital Hamburg-Eppendorf, 20246 Hamburg, Germany.
authors.forename: Hans
authors.initials: H
authors.lastname: Will
authors.affiliation: Department of Infection Biology, Faculty of Medicine, University of Tsukuba, Tsukuba 305-8575, Japan.
authors.forename: Kyosuke
authors.initials: K
authors.lastname: Nagata
authors.affiliation: Microbiologie Fondamentale et Pathogénicité, MFP CNRS UMR 5234, Université de Bordeaux, Bordeaux 33076, France. Electronic address: harald.wodrich@u-bordeaux.fr.
authors.forename: Harald
authors.initials: H
authors.lastname: Wodrich
mesh_terms: D000256:Adenoviridae / Q000235:genetics* / Q000502:physiology; D000257:Adenoviridae Infections / Q000276:immunology; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000276:immunology*; D019084:Fluorescent Antibody Technique, Indirect; D016679:Genome, Viral; D054884:Host-Pathogen Interactions; D006801:Humans; D007372:Interferons / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D009504:Neutrophils / Q000166:cytology / Q000821:virology; D009687:Nuclear Proteins / Q000235:genetics / Q000276:immunology*; D010750:Phosphoproteins / Q000276:immunology*; D014157:Transcription Factors / Q000276:immunology*; D014779:Virus Replication
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C503048:PHF13 protein, human; D010750:Phosphoproteins; D014157:Transcription Factors; C076700:IFI16 protein, human; D007372:Interferons
keywords: Adenovirus; Antiviral response; IFI16; Intrinsic immunity; PHF13/SPOC1; PML nuclear body