title: mTOR kinase is needed for the development and stabilization of dendritic arbors in newly born olfactory bulb neurons.
abstract: Neurogenesis is the process of neuron generation, which occurs not only during embryonic development but also in restricted niches postnatally. One such region is called the subventricular zone (SVZ), which gives rise to new neurons in the olfactory bulb (OB). Neurons that are born postnatally migrate through more complex territories and integrate into fully functional circuits. Therefore, differences in the differentiation of embryonic and postnatally born neurons may exist. Dendritogenesis is an important process for the proper formation of future neuronal circuits. Dendritogenesis in embryonic neurons cultured in vitro was shown to depend on the mammalian target of rapamycin (mTOR). Still unknown, however, is whether mTOR could regulate the dendritic arbor morphology of SVZ-derived postnatal OB neurons under physiological conditions in vivo. The present study used in vitro cultured and differentiated SVZ-derived neural progenitors and found that both mTOR complex 1 and mTOR complex 2 were required for the dendritogenesis of SVZ-derived neurons. Furthermore, using a combination of in vivo electroporation of neural stem cells in the SVZ and genetic and pharmacological inhibition of mTOR, it was found that mTOR was crucial for the growth of basal and apical dendrites in postnatally born OB neurons under physiological conditions and contributed to the stabilization of their basal dendrites. © 2016 Wiley Periodicals, Inc. Develop Neurobiol 76: 1308-1327, 2016.
authors.affiliation: International Institute of Molecular and Cell Biology, 4 Ks. Trojdena St, Warsaw, 02-109, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Skalecka
authors.affiliation: International Institute of Molecular and Cell Biology, 4 Ks. Trojdena St, Warsaw, 02-109, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Liszewska
authors.affiliation: Département De Mathématiques, Collège Montmorency, 475 Boulevard De L'Avenir, Laval, Quebec, H7N 5H9, Canada.
authors.forename: Robert
authors.initials: R
authors.lastname: Bilinski
authors.affiliation: Patrick Wild Centre and Centre for Integrative Physiology, University of Edinburgh, Hugh Robson Building George Square, Edinburgh, EH8 9XD, United Kingdom.
authors.forename: Christos
authors.initials: C
authors.lastname: Gkogkas
authors.affiliation: Department of Biochemistry, Goodman Cancer Research Centre, McGill University, 1160 Pine Avenue West, Montreal, Quebec, H3A 1A3, Canada.
authors.forename: Arkady
authors.initials: A
authors.lastname: Khoutorsky
authors.affiliation: International Institute of Molecular and Cell Biology, 4 Ks. Trojdena St, Warsaw, 02-109, Poland.
authors.forename: Anna R
authors.initials: AR
authors.lastname: Malik
authors.affiliation: Department of Biochemistry, Goodman Cancer Research Centre, McGill University, 1160 Pine Avenue West, Montreal, Quebec, H3A 1A3, Canada.
authors.forename: Nahum
authors.initials: N
authors.lastname: Sonenberg
authors.affiliation: International Institute of Molecular and Cell Biology, 4 Ks. Trojdena St, Warsaw, 02-109, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Jaworski
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D002552:Cerebral Ventricles / Q000502:physiology; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009830:Olfactory Bulb / Q000254:growth & development*; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*
chemical_list: D058570:TOR Serine-Threonine Kinases
keywords: dendritogenesis; in vivo electroporation; mTOR; olfactory bulb; postnatal neurogenesis