title: Does hyperbaric oxygen therapy reduce the effects of ischemia on colonic anastomosis in laparoscopic colon resection?
abstract: BACKGROUND
  An increase in intra-abdominal pressure causes a decrease in the splanchnic blood flow and the intramucosal pH of the bowel, as well as increasing the risk of ischemia in the colon. The aim of the present study is to evaluate the effect of hyperbaric oxygen therapy (HBOT) on the ischemia caused by laparoscopy in colonic anastomosis in an experimental model of laparoscopic colonic surgery.
  
  
  METHODS
  We divided 30 male Wistar albino rats into three groups: Group A was the control (open colon anastomosis); Group B received LCA (laparoscopic colon anastomosis); while Group C received both LCA and HBOT. Each group contained ten animals. We placed Group C (LCA and HBOT) in an experimental hyperbaric chamber into which we administered pure oxygen at 2.1 atmospheres absolute 100% oxygen for 60 min for ten consecutive days.
  
  
  RESULTS
  The anastomotic bursting pressure value was found to be higher in the open surgery group (226 ± 8.8) (Group A). The result for Group C (213 ± 27), which received HBOT, was better than that for Group B (197 ± 27). However, there was no statistically significant difference between Group B and Group C. Group A showed better healing than the other groups, while significant differences in the fibroblast proliferation scores were found between Groups A and B. In terms of tissue hydroxyproline levels, a significant difference was found between Groups A and B and between Groups A and C, but not between Groups B and C.
  
  
  CONCLUSIONS
  HBOT increases the oxygen level in the injured tissue. Although HBOT might offer several advantages, it had only a limited effect on the healing of colonic anastomosis in rats with increased intra-abdominal pressure in our study.
  
  
  BACKGROUND
  Anastomosis, Colon, Hyperbaric Oxygen Treatment, Oxidative Stress.
authors.forename: Seyfi
authors.initials: S
authors.lastname: Emir
authors.forename: Sibel Ozkan
authors.initials: SO
authors.lastname: Gurdal
authors.forename: Selim
authors.initials: S
authors.lastname: Sozen
authors.forename: Ilhan
authors.initials: I
authors.lastname: Bali
authors.forename: Ebru
authors.initials: E
authors.lastname: Yesildag
authors.forename: Atilla
authors.initials: A
authors.lastname: Celik
authors.forename: Savas
authors.initials: S
authors.lastname: Guzel
authors.forename: Onder
authors.initials: O
authors.lastname: Sahin
authors.forename: Hakan
authors.initials: H
authors.lastname: Ay
authors.forename: Birol
authors.initials: B
authors.lastname: Topcu
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D002455:Cell Division; D003106:Colon / Q000098:blood supply / Q000601:surgery*; D005347:Fibroblasts / Q000378:metabolism; D006909:Hydroxyproline / Q000378:metabolism; D006931:Hyperbaric Oxygenation; D007511:Ischemia / Q000517:prevention & control* / Q000628:therapy; D010535:Laparoscopy / Q000379:methods*; D008297:Male; D018384:Oxidative Stress; D011183:Postoperative Complications / Q000517:prevention & control* / Q000628:therapy; D011312:Pressure; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013152:Splanchnic Circulation; D013718:Tensile Strength; D014945:Wound Healing
chemical_list: D006909:Hydroxyproline