title: Production of Cloned Korean Native Pig by Somatic Cell Nuclear Transfer.
abstract: The Korean native pig (KNP) have been considered as animal models for animal biotechnology research because of their relatively small body size and their presumably highly inbred status due to the closed breeding program. However, little is reported about the use of KNP for animal biotechnology researches. This study was performed to establish the somatic cell nuclear transfer (SCNT) protocol for the production of swine leukocyte antigens (SLA) homotype-defined SCNT KNP. The ear fibroblast cells originated from KNP were cultured and used as donor cell. After thawing, the donor cells were cultured for 1 hour with 15 μM roscovitine prior to the nuclear transfer. The numbers of reconstructed and parthenogenetic embryos transferred were 98 ± 35.2 and 145 ± 11.2, respectively. The pregnancy and delivery rate were 3/5 (60%) and 2/5 (40%). One healthy SLA homotype-defined SCNT KNP was successfully generated. The recipient-based individual cloning efficiency ranged from 0.65 to 1.08%. Taken together, it can be postulated that the methodological establishment of the production of SLA homotype-defined cloned KNP can be applied to the generation of transgenic cloned KNP as model animals for human disease and xenotransplantation researches.
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, Wanju 565-851, Korea.
authors.forename: In-Sul
authors.initials: IS
authors.lastname: Hwang
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, Wanju 565-851, Korea.
authors.forename: Dae-Jin
authors.initials: DJ
authors.lastname: Kwon
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, Wanju 565-851, Korea.
authors.forename: Keun Bong
authors.initials: KB
authors.lastname: Oh
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, Wanju 565-851, Korea.
authors.forename: Sun-A
authors.initials: SA
authors.lastname: Ock
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, Wanju 565-851, Korea.
authors.forename: Hak-Jae
authors.initials: HJ
authors.lastname: Chung
authors.affiliation: Subtropical Livestock Research Institute, NIAS, Jeju 690-150, Korea.
authors.forename: In-Cheol
authors.initials: IC
authors.lastname: Cho
authors.affiliation: Functional Genomics Research Center, KRIBB, Daejeon 305-806, Korea.
authors.forename: Jeong-Woong
authors.initials: JW
authors.lastname: Lee
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, Wanju 565-851, Korea.
authors.forename: Gi-Sun
authors.initials: GS
authors.lastname: Im
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, Wanju 565-851, Korea.
authors.forename: Seongsoo
authors.initials: S
authors.lastname: Hwang
keywords: Animal model; Cloning efficiency; Korean native pig; Somatic cell nuclear transfer; Swine leukocyte antigens