title: IDENTIFICATION OF THE HIGH-TEMPERATURE RESPONSE GENES FROM PORPHYRA SERIATA (RHODOPHYTA) EXPRESSION SEQUENCE TAGS AND ENHANCEMENT OF HEAT TOLERANCE OF CHLAMYDOMONAS (CHLOROPHYTA) BY EXPRESSION OF THE PORPHYRA HTR2 GENE(1).
abstract: Temperature is one of the major environmental factors that affect the distribution, growth rate, and life cycle of intertidal organisms, including red algae. In an effort to identify the genes involved in the high-temperature tolerance of Porphyra, we generated 3,979 expression sequence tags (ESTs) from gametophyte thalli of P. seriata Kjellm. under normal growth conditions and high-temperature conditions. A comparison of the ESTs from two cDNA libraries allowed us to identify the high temperature response (HTR) genes, which are induced or up-regulated as the result of high-temperature treatment. Among the HTRs, HTR2 encodes for a small polypeptide consisting of 144 amino acids, which is a noble nuclear protein. Chlamydomonas expressing the Porphyra HTR2 gene shows higher survival and growth rates than the wild-type strain after high-temperature treatment. These results suggest that HTR2 may be relevant to the tolerance of high-temperature stress conditions, and this Porphyra EST data set will provide important genetic information for studies of the molecular basis of high-temperature tolerance in marine algae, as well as in Porphyra.
authors.affiliation: Department of Biology Education, Chonnam National University, Kwagnju, 500-757, KoreaPlant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, KoreaGenome Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, KoreaSeaweed Research Center, National Fisheries Research and Development Institute, Mokpo, 530-931, Korea.
authors.forename: Euicheol
authors.initials: E
authors.lastname: Kim
authors.affiliation: Department of Biology Education, Chonnam National University, Kwagnju, 500-757, KoreaPlant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, KoreaGenome Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, KoreaSeaweed Research Center, National Fisheries Research and Development Institute, Mokpo, 530-931, Korea.
authors.forename: Hong-Sil
authors.initials: HS
authors.lastname: Park
authors.affiliation: Department of Biology Education, Chonnam National University, Kwagnju, 500-757, KoreaPlant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, KoreaGenome Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, KoreaSeaweed Research Center, National Fisheries Research and Development Institute, Mokpo, 530-931, Korea.
authors.forename: Youngja
authors.initials: Y
authors.lastname: Jung
authors.affiliation: Department of Biology Education, Chonnam National University, Kwagnju, 500-757, KoreaPlant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, KoreaGenome Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, KoreaSeaweed Research Center, National Fisheries Research and Development Institute, Mokpo, 530-931, Korea.
authors.forename: Dong-Woog
authors.initials: DW
authors.lastname: Choi
authors.affiliation: Department of Biology Education, Chonnam National University, Kwagnju, 500-757, KoreaPlant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, KoreaGenome Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, KoreaSeaweed Research Center, National Fisheries Research and Development Institute, Mokpo, 530-931, Korea.
authors.forename: Won-Joong
authors.initials: WJ
authors.lastname: Jeong
authors.affiliation: Department of Biology Education, Chonnam National University, Kwagnju, 500-757, KoreaPlant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, KoreaGenome Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, KoreaSeaweed Research Center, National Fisheries Research and Development Institute, Mokpo, 530-931, Korea.
authors.forename: Hong-Seog
authors.initials: HS
authors.lastname: Park
authors.affiliation: Department of Biology Education, Chonnam National University, Kwagnju, 500-757, KoreaPlant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, KoreaGenome Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, KoreaSeaweed Research Center, National Fisheries Research and Development Institute, Mokpo, 530-931, Korea.
authors.forename: Mi Sook
authors.initials: MS
authors.lastname: Hwang
authors.affiliation: Department of Biology Education, Chonnam National University, Kwagnju, 500-757, KoreaPlant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, KoreaGenome Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, KoreaSeaweed Research Center, National Fisheries Research and Development Institute, Mokpo, 530-931, Korea.
authors.forename: Eun-Jeong
authors.initials: EJ
authors.lastname: Park
authors.affiliation: Department of Biology Education, Chonnam National University, Kwagnju, 500-757, KoreaPlant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, KoreaGenome Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, KoreaSeaweed Research Center, National Fisheries Research and Development Institute, Mokpo, 530-931, Korea.
authors.forename: Yong-Gun
authors.initials: YG
authors.lastname: Gong
keywords: HTR2; Porphyra seriata; expression sequence tags; high-temperature response gene; high-temperature stress