title: Association between IL17A and IL17F polymorphisms and risk of Henoch-Schonlein purpura in Chinese children.
abstract: Previous studies suggested that interleukin-17 and Th17 cell play an important role in the pathogenesis of childhood Henoch-Schonlein purpura (HSP). The purpose of our study is to elucidate whether the IL17A and IL17F gene polymorphisms are susceptibility genes for the development of HSP in Chinese children. A total of 148 HSP patients and 202 controls were enrolled for analyzing the single nucleotide polymorphisms (SNP) of IL17A (rs2275913, rs8193037 and rs3819025) and IL17F (rs763780 and rs9463772). TaqMan Real-Time polymerase chain reaction method was used in SNP genotyping. Compared to the healthy controls, the IL17A rs2275913 variant allele A showed a significant association with HSP [odds ratio (OR) 0.70; 95 % CI 0.51-0.94, P = 0.018]. Genotyping analysis demonstrated rs2275913 was associated with a decreased HSP risk (G/A vs. G/G: OR 0.56; 95 % CI 0.33-0.95; A/A vs. G/G: OR 0.46; 95 % CI 0.24-0.86; P = 0.032). Also, our findings showed that the A allele of IL17A rs3819025 was associated with a higher risk of HSP nephritis (OR 1.61; 95 % CI 1.00-2.58; P = 0.047). In addition, a risk haplotype of IL17A (GGA) was found (OR 1.84; 95 % CI 1.17-2.88; P = 0.008). However, no significant differences between HSP patients and healthy controls were observed when comparing genotype, allele or haplotype frequencies of the IL17F rs763780 and rs9463772 polymorphisms. In this study, we confirmed that the rs2275913 polymorphism of the IL17A gene was associated with susceptibility to HSP in Chinese children. However, there was no relationship between IL17F rs763780 and rs9463772 polymorphisms and HSP susceptibility.
authors.affiliation: Department of Clinical Laboratory, Children's Hospital of Zhejiang University School of Medicine, Hangzhou, 310003, Zhejiang, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Clinical Laboratory, Children's Hospital of Zhejiang University School of Medicine, Hangzhou, 310003, Zhejiang, People's Republic of China.
authors.forename: Yanxiang
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Department of Clinical Laboratory, Children's Hospital of Zhejiang University School of Medicine, Hangzhou, 310003, Zhejiang, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Nephrology, Children's Hospital of Zhejiang University School of Medicine, Hangzhou, 310003, Zhejiang, People's Republic of China. zjxh2015@aliyun.com.
authors.forename: Haidong
authors.initials: H
authors.lastname: Fu
authors.affiliation: Department of Clinical Laboratory, Children's Hospital of Zhejiang University School of Medicine, Hangzhou, 310003, Zhejiang, People's Republic of China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, Children's Hospital of Zhejiang University School of Medicine, Hangzhou, 310003, Zhejiang, People's Republic of China.
authors.forename: Hongqiang
authors.initials: H
authors.lastname: Shen
authors.affiliation: Department of Clinical Laboratory, Children's Hospital of Zhejiang University School of Medicine, Hangzhou, 310003, Zhejiang, People's Republic of China.
authors.forename: Xiucui
authors.initials: X
authors.lastname: Han
mesh_terms: D000367:Age Factors; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D011695:IgA Vasculitis / Q000175:diagnosis / Q000208:ethnology / Q000235:genetics*; D020381:Interleukin-17 / Q000235:genetics*; D015810:Linkage Disequilibrium; D008297:Male; D016017:Odds Ratio; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction; D012307:Risk Factors
chemical_list: D005819:Genetic Markers; C508717:IL17A protein, human; C440021:IL17F protein, human; D020381:Interleukin-17
keywords: Henoch–Schonlein purpura; Inflammation; Interleukin-17; Polymorphism