title: Regulation of Exacerbated Immune Responses in Human Peripheral Blood Cells by Hydrolysed Egg White Proteins.
abstract: The anti-allergic potential of egg white protein hydrolysates (from ovalbumin, lysozyme and ovomucoid) was evaluated as their ability to hinder cytokine and IgE production by Th2-skewed human peripheral blood mononuclear cells (PBMCs), as well as the release of pro-inflammatory factors and generation of reactive oxygen species from Th1-stimulated peripheral blood leukocytes (PBLs). The binding to IgE of egg allergic patients was determined and the peptides present in the hydrolysates were identified. The hydrolysates with alcalase down-regulated the production of Th2-biased cytokines and the secretion of IgE to the culture media of Th2-skewed PBMCs, and they significantly neutralized oxidative stress in PBLs. The hydrolysates of ovalbumin and ovomucoid with pepsin helped to re-establish the Th1/Th2 balance in Th2-biased PBMCs, while they also inhibited the release of pro-inflammatory mediators and reduced oxidative stress in PBLs treated with inflammatory stimuli. The hydrolysates with alcalase, in addition to equilibrating Th2 differentiation, exhibited a low IgE-binding. Therefore, they would elicit mild allergic reactions while retaining T cell-stimulating abilities, which might correlate with an anti-allergic benefit.
authors.affiliation: Instituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM), Nicolás Cabrera 9, 28049, Madrid, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Lozano-Ojalvo
authors.affiliation: Instituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM), Nicolás Cabrera 9, 28049, Madrid, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Molina
authors.affiliation: Instituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM), Nicolás Cabrera 9, 28049, Madrid, Spain.
authors.forename: Rosina
authors.initials: R
authors.lastname: López-Fandiño
mesh_terms: D001773:Blood Cells / Q000276:immunology*; D002851:Chromatography, High Pressure Liquid; D016207:Cytokines / Q000378:metabolism; D004527:Egg Proteins / Q000502:physiology*; D004529:Egg White / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006868:Hydrolysis; D017382:Reactive Oxygen Species / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D016207:Cytokines; D004527:Egg Proteins; D017382:Reactive Oxygen Species