title: Sol-gel approach for extracting highly versatile aspirin and its metabolites using MISPE followed by GC-MS/MS analysis.
abstract: OBJECTIVE
  Aspirin is known to be a salicylate drug widely used as an analgesic, antipyretic and anti-inflammatory drug.
  
  
  METHODS
  Sol-gel based nanosized molecularly imprinted polymer (nMIP) has been synthesized for extraction of aspirin and its metabolites in urine followed by GC-MS/MS analysis.
  
  
  RESULTS
  Binding affinity of nMIP and nonimprinted polymer was found to be in the range of 70-95% and 29-45%, respectively. LOD and LOQ of aspirin and its metabolites were found to be in the range of 0.63-2.4 ng/ml and 2.07-7.68 ng/ml, respectively.
  
  
  CONCLUSIONS
  The developed method was found to be applicable for routine analysis of aspirin and its metabolites in biological samples.
authors.affiliation: Analytical Chemistry Laboratory, Regulatory Toxicology Group, CSIR-IITR, MG Marg, Lucknow 226001, India.|Academy of Scientific & Innovative Research (AcSIR), CSIR-IITR, MG Marg, Lucknow 226001, India.
authors.forename: Tejasvi
authors.initials: T
authors.lastname: Bhatia
authors.affiliation: Analytical Chemistry Laboratory, Regulatory Toxicology Group, CSIR-IITR, MG Marg, Lucknow 226001, India.|Academy of Scientific & Innovative Research (AcSIR), CSIR-IITR, MG Marg, Lucknow 226001, India.
authors.forename: Manoj Kumar
authors.initials: MK
authors.lastname: Gupta
authors.affiliation: Analytical Chemistry Laboratory, Regulatory Toxicology Group, CSIR-IITR, MG Marg, Lucknow 226001, India.
authors.forename: Pratibha
authors.initials: P
authors.lastname: Singh
authors.affiliation: Analytical Chemistry Laboratory, Regulatory Toxicology Group, CSIR-IITR, MG Marg, Lucknow 226001, India.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Chauhan
authors.affiliation: CIF Facility, CSIR-IITR, MG Marg, Lucknow 226001, India.
authors.forename: Prem Narain
authors.initials: PN
authors.lastname: Saxena
authors.affiliation: Analytical Chemistry Laboratory, Regulatory Toxicology Group, CSIR-IITR, MG Marg, Lucknow 226001, India.|Academy of Scientific & Innovative Research (AcSIR), CSIR-IITR, MG Marg, Lucknow 226001, India.|Pesticide Toxicology Laboratory, Regulatory Toxicology Group, CSIR-IITR, M G Marg, Lucknow 226001, India.
authors.forename: Mohana Krishna Reddy
authors.initials: MK
authors.lastname: Mudiam
mesh_terms: D001241:Aspirin / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D005782:Gels / Q000737:chemistry*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry; D052616:Solid Phase Extraction
chemical_list: D005782:Gels; D011108:Polymers; D001241:Aspirin
keywords: GC–MS/MS; aspirin and its metabolites; molecular imprinted polymer; sol-gel polymerization