title: Development of Marker-Free Transgenic Potato Tubers Enriched in Caffeoylquinic Acids and Flavonols.
abstract: Potato (Solanum tuberosum L.) is a major crop worldwide that meets human economic and nutritional requirements. Potato has several advantages over other crops: easy to cultivate and store, cheap to consume, and rich in a variety of secondary metabolites. In this study, we generated three marker-free transgenic potato lines that expressed the Arabidopsis thaliana flavonol-specific transcriptional activator AtMYB12 driven by the tuber-specific promoter Patatin. Marker-free potato tubers displayed increased amounts of caffeoylquinic acids (CQAs) (3.35-fold increases on average) and flavonols (4.50-fold increase on average). Concentrations of these metabolites were associated with the enhanced expression of genes in the CQA and flavonol biosynthesis pathways. Accumulation of CQAs and flavonols resulted in 2-fold higher antioxidant capacity compared to wild-type potatoes. Tubers from these marker-free transgenic potatoes have therefore improved antioxidant properties.
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, Shandong Agricultural University , Taian 271018, Shandong, P. R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory for Rare Disease of Shandong Province, Institute of Materia Medica, Shandong Academy of Medical Sciences , Jinan 250062, Shandong, P. R. China.
authors.forename: Wenzhao
authors.initials: W
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, Shandong Agricultural University , Taian 271018, Shandong, P. R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, Shandong Agricultural University , Taian 271018, Shandong, P. R. China.
authors.forename: Ru
authors.initials: R
authors.lastname: Jia
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, Shandong Agricultural University , Taian 271018, Shandong, P. R. China.
authors.forename: Lianjie
authors.initials: L
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, Shandong Agricultural University , Taian 271018, Shandong, P. R. China.
authors.forename: Shaoli
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, Shandong Agricultural University , Taian 271018, Shandong, P. R. China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Wang
authors.affiliation: Biotechnology Research Center, China Three Gorges University , Yichang City 443002, Hubei, P. R. China.
authors.forename: Xiangling
authors.initials: X
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, Shandong Agricultural University , Taian 271018, Shandong, P. R. China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Chu
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, Shandong Agricultural University , Taian 271018, Shandong, P. R. China.|Shandong YuTai Biotechnology Company , Taian 271018, Shandong, P. R. China.
authors.forename: Changxiang
authors.initials: C
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, Shandong Agricultural University , Taian 271018, Shandong, P. R. China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Ding
mesh_terms: D044948:Flavonols / Q000032:analysis / Q000096:biosynthesis*; D035281:Plant Tubers / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D011198:Solanum tuberosum / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D044948:Flavonols; C472707:caffeoylquinic acid; D011801:Quinic Acid
keywords: MYB transcription factors; antioxidant capacity; marker-free transgenic potato; phenylpropanoid improved