title: Elucidation of the role of oleosin in off-flavour generation in soymeal through supercritical CO₂ and biotic elicitor treatments.
abstract: Defatting soybean by sophisticated oil extraction method utilising supercritical CO2 resulted in a significant decrease in the residual phospholipids (PLs) compared with soymeal obtained by conventional cold percolation method utilising hexane as the extraction solvent. Interestingly, the levels of residual PLs showed a proportionate relationship with thiobarbituric acid (TBA) number, an indicator of lipid peroxidation responsible for off-flavour generation. Furthermore, two oleosins (18 and 24 kDa) were isolated from the oil bodies extracted from soybean seeds and positively characterised for phospholipase A2 (PLA2) activity, suggesting their plausible involvement in off-flavour generation in soymeal. The treatment of soybean seeds, before oil extraction, with different concentrations of biotic elicitors such as chitosan and jasmonic acid also significantly reduced the levels of residual PLs as well as the TBA number. The biotic elicitor treatment could thus prove to be an important strategy for the reduction of off-flavour in protein-rich soymeal.
authors.affiliation: Division of Biochemistry, Indian Agricultural Research Institute, New Delhi 110012, India. Electronic address: swetaarunbiochem@gmail.com.
authors.forename: Sweta
authors.initials: S
authors.lastname: Kumari
authors.affiliation: Division of Biochemistry, Indian Agricultural Research Institute, New Delhi 110012, India.
authors.forename: Lucia Joseph
authors.initials: LJ
authors.lastname: Memba
authors.affiliation: Division of Biochemistry, Indian Agricultural Research Institute, New Delhi 110012, India. Electronic address: dahuja.anil@gmail.com.
authors.forename: Anil
authors.initials: A
authors.lastname: Dahuja
authors.affiliation: Division of Biochemistry, Indian Agricultural Research Institute, New Delhi 110012, India.
authors.forename: T
authors.initials: T
authors.lastname: Vinutha
authors.affiliation: Division of Agricultural Chemicals, Indian Agricultural Research Institute, New Delhi 110012, India.
authors.forename: Supradip
authors.initials: S
authors.lastname: Saha
authors.affiliation: Division of Biochemistry, Indian Agricultural Research Institute, New Delhi 110012, India.
authors.forename: Archana
authors.initials: A
authors.lastname: Sachdev
mesh_terms: D002245:Carbon Dioxide; D010938:Plant Oils / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry*
chemical_list: D010938:Plant Oils; D010940:Plant Proteins; D002245:Carbon Dioxide
keywords: Biotic elicitors; Off-flavour; Oleosin; Phospholipase A(2) (PLA(2)); Phospholipids (PLs); Soymeal; Supercritical carbon dioxide (SC-CO(2)); Thiobarbituric acid (TBA)