title: Genotyping and follow-up of HR-HPV types detected by self-sampling in women from low socioeconomic groups not participating in regular cervical cancer screening in France.
abstract: BACKGROUND
  HPV vaginal self-sampling can be an alternative for women refusing cytological screening.
  
  
  OBJECTIVE
  To describe HR-HPV types in 35-69 years old women from low socioeconomic groups not attending regular cytological screening in Marseille, France.
  
  
  METHODS
  A cervical screening campaign using HR-HPV self-sampling including 22,702 women aged 35-69 years living in low socioeconomic districts of Marseille was organized. A cytological and/or histological follow-up was undertaken for a subset of women harboring HR-HPV types. Abbott RealTime High Risk HPV test was used for screening, while INNO-LiPA HPV Genotyping Extra assay was used for genotyping.
  
  
  RESULTS
  4245 self-samplings were performed (participation rate, 18.7%) out of which 609 (14.3%) were HR-HPV+ by the screening test including 114HPV 16 (18.7%), 41HPV 18 (6.7%), 454HR-HPVnon-16/18 (75.4%). A sample of 260 out of the 454HR-HPVnon-16/18 were genotyped by INNO-LiPA which revealed HPV52 (35%), 66 (22.6%), 51 (19.6%), 31 (15.7%), 39 (13%), 56 (10.4%), and 53, 35, 59, 33, 58, 82, 45, 68, 73 (<10% each). At month 12, a 2nd self-collection kit was sent to 274 of 609HR-HPV+ women who did not have a Pap-test previously performed on them. Of these 274 women, 130 provided a sample for HPV testing; one was uninterpretable, 56 were HPV negative, and 73 were HR-HPV+ (10HPV16+, 3HPV18+, 60HR-HPVnon-16/18+). Of the 345 women with cytological and/or histological follow-up 19 (5.5%) had ≥CIN2 lesions, (11 were HPV16+ and 8 were HR-HPVnon-16/18).
  
  
  CONCLUSIONS
  This study illustrates the potential efficacy of self-sampling as a cancer screening strategy for socioeconomically deprived women who do not participate in regular Pap screening programs.
authors.affiliation: Fondation Institut Hospitalo-Universitaire Méditerranée Infection, Pôle des Maladies Infectieuses et Tropicales Clinique et Biologique, Fédération de Bactériologie-Hygiène-Virologie, Centre Hospitalo-Universitaire Timone, 264 rue Saint-Pierre 13385, Marseille Cedex 05, France. Electronic address: ctamalet@ap-hm.fr.
authors.forename: Catherine
authors.initials: C
authors.lastname: Tamalet
authors.affiliation: Laboratoire Alphabio, Hôpital Européen, 6 Rue Désirée Clary, 13003 Marseille, France. Electronic address: Philippe.halfon@alphabio.fr.
authors.forename: Philippe
authors.initials: P
authors.lastname: Halfon
authors.affiliation: Association pour la recherche et le dépistage des cancers du sein, du col de l'utérus et des cancers colorectaux (Arcades), Parc MÛre, Bâtiment A, 16 Bd des Aciéries, CS 90006, 13395 Marseille Cedex 10, France. Electronic address: laurence.leretraite@arcades-depistages.com.
authors.forename: Laurence Le
authors.initials: LL
authors.lastname: Retraite
authors.affiliation: Laboratoire départemental d'analyses, Technopôle de Château, Gombert Marseille, France. Electronic address: anne.grob@cg13.fr.
authors.forename: Anne
authors.initials: A
authors.lastname: Grob
authors.affiliation: Association pour la recherche et le dépistage des cancers du sein, du col de l'utérus et des cancers colorectaux (Arcades), Parc MÛre, Bâtiment A, 16 Bd des Aciéries, CS 90006, 13395 Marseille Cedex 10, France. Electronic address: secretariat.col@arcades-depistages.com.
authors.forename: François Xavier
authors.initials: FX
authors.lastname: Leandri
authors.affiliation: Association pour la recherche et le dépistage des cancers du sein, du col de l'utérus et des cancers colorectaux (Arcades), Parc MÛre, Bâtiment A, 16 Bd des Aciéries, CS 90006, 13395 Marseille Cedex 10, France. Electronic address: secretariat.col@arcades-depistages.com.
authors.forename: Patrice
authors.initials: P
authors.lastname: Heid
authors.affiliation: Fondation JDB Prevention Cancer, Fontaine les Briis 91640, France. Electronic address: Helene.Sancho-Garnier@icm.unicancer.fr.
authors.forename: Helene
authors.initials: H
authors.lastname: Sancho-Garnier
authors.affiliation: Association pour la recherche et le dépistage des cancers du sein, du col de l'utérus et des cancers colorectaux (Arcades), Parc MÛre, Bâtiment A, 16 Bd des Aciéries, CS 90006, 13395 Marseille Cedex 10, France. Electronic address: secretariat.col@arcades-depistages.com.
authors.forename: Lucien
authors.initials: L
authors.lastname: Piana
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D005602:France / Q000453:epidemiology; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000453:epidemiology* / Q000821:virology*; D012646:Self Administration; D012923:Social Class; D013048:Specimen Handling / Q000379:methods*; D014621:Vagina / Q000821:virology
keywords: Cervical cancer screening; Follow-up; HPV genotyping; High-risk human papillomavirus (HPV); Self-sampling