title: The plasma miR-125a, miR-361 and miR-133a are promising novel biomarkers for Late-Onset Hypogonadism.
abstract: Circulating miRNAs have been shown to serve as diagnostic/prognostic biomarkers in cancers and other diseases. However, the role of plasma miRNAs in Late-onset hypogonadism (LOH) diagnosis is still unknown. Using Illumina HiSeq2000 sequencing at discovery phase, and then two-step validated by reverse transcriptase polymerase chain reaction (RT-PCR) assays in verification phases. We verified that the expression levels of miR-125a-5p, miR-361-5p and miR-133a-3p were significantly altered in LOH group compared to the control group. The area under the receiver operating characteristic (ROC) curve (AUC) is 0.682, 0.698 and 0.765, respectively. The combination of three miRNAs showed a larger AUC (0.835) that was more efficient for the diagnosis of LOH. Among three miRNAs, miR-133a-3p had the best diagnostic value for LOH with 68.2% sensitivity and 77.3% specificity. Regression analyses show that miR-133a-3p level was negatively associated with the ageing males' symptoms (AMS) scale. However, miR-361-5p level was positively associated with serum testosterone concentrations. In summary, plasma miRNAs are differentially expressed between LOH and healthy controls. We validated three miRNAs that could act as novel biomarkers for diagnosis of LOH. These miRNAs may be involved in the development of LOH. However, further large and functional studies are warranted to confirm our findings.
authors.affiliation: Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan 430030, China.|Center of Reproductive Medicine, General Hospital of Ningxia Medical University, Shengli South Street 804, Yinchuan, Ningxia 750004, China.
authors.forename: Yao-Ping
authors.initials: YP
authors.lastname: Chen
authors.affiliation: Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan 430030, China.|Department of Histology and Embryology, School of Medicine, Shihezi University, North 2nd Road 59, Shihezi, Xinjiang 832002, China.
authors.forename: Ju
authors.initials: J
authors.lastname: Wang
authors.affiliation: Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan 430030, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
authors.affiliation: Department of Andrology, Jinling Hospital, School of Medicine, Nanjing University, East Zhongshan Road 305, Nanjing 210002, China.
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Shang
authors.affiliation: Emergency Department, General Hospital of Ningxia Medical University, Shengli South Street 804, Yinchuan, Ningxia 750004, China.
authors.forename: Hui-Qin
authors.initials: HQ
authors.lastname: Wu
authors.affiliation: Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan 430030, China.
authors.forename: Xing-Rong
authors.initials: XR
authors.lastname: Qing
authors.affiliation: Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan 430030, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Fang
authors.affiliation: Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan 430030, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan 430030, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Shang
authors.affiliation: Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan 430030, China.|Wuhan Tongji Reproductive Medicine Hospital, Sanyang Road 128, Wuhan 430013, China.
authors.forename: Hong-Gang
authors.initials: HG
authors.lastname: Li
authors.affiliation: Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan 430030, China.|Wuhan Tongji Reproductive Medicine Hospital, Sanyang Road 128, Wuhan 430013, China.
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Zhang
authors.affiliation: Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan 430030, China.|Wuhan Tongji Reproductive Medicine Hospital, Sanyang Road 128, Wuhan 430013, China.
authors.forename: Huang-Tao
authors.initials: HT
authors.lastname: Guan
authors.affiliation: School of Public Health, Zunyi Medical University, Dalian Road 201, Zunyi, Guizhou 563099, China.
authors.forename: Yuan-Zhong
authors.initials: YZ
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Male Reproductive Health, National Health and Family Planning Commission, National Research Institute for Family Planning, Da Hui Si Rd 12, Hai Dian District, Beijing 100081, China.
authors.forename: Yi-Qun
authors.initials: YQ
authors.lastname: Gu
authors.affiliation: Guangzhou Institute for Population and Family Planning, Xin Shi Xin Da road 93, Baiyun District, Guangzhou 510410, China.
authors.forename: Wei-Xiong
authors.initials: WX
authors.lastname: Wu
authors.affiliation: Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan 430030, China.|Wuhan Tongji Reproductive Medicine Hospital, Sanyang Road 128, Wuhan 430013, China.
authors.forename: Cheng-Liang
authors.initials: CL
authors.lastname: Xiong
mesh_terms: D017668:Age of Onset; D015415:Biomarkers / Q000097:blood*; D016022:Case-Control Studies; D006801:Humans; D007006:Hypogonadism / Q000097:blood / Q000175:diagnosis*; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged
chemical_list: D015415:Biomarkers; D035683:MicroRNAs