title: Lactobacillus rhamnosus could inhibit Porphyromonas gingivalis derived CXCL8 attenuation.
abstract: An increasing body of evidence suggests that the use of probiotic bacteria is a promising intervention approach for the treatment of inflammatory diseases with a polymicrobial etiology. P. gingivalis has been noted to have a different way of interacting with the innate immune response of the host compared to other pathogenic bacteria, which is a recognized feature that inhibits CXCL8 expression.
  
  
  OBJECTIVE
  The aim of the study was to determine if P. gingivalis infection modulates the inflammatory response of gingival stromal stem cells (G-MSSCs), including the release of CXCL8, and the expression of TLRs and if immunomodulatory L. rhamnosus ATCC9595 could prevent CXCL8 inhibition in experimental inflammation.
  
  
  METHODS
  G-MSSCs were pretreated with L. rhamnosus ATCC9595 and then stimulated with P. gingivalis ATCC33277. CXCL8 and IL-10 levels were investigated with ELISA and the TLR-4 and 2 were determined through flow cytometer analysis.
  
  
  RESULTS
  CXCL8 was suppressed by P. gingivalis and L. rhamnosus ATCC9595, whereas incubation with both strains did not abolish CXCL8. L. rhamnosus ATCC9595 scaled down the expression of TLR4 and induced TLR2 expression when exposed to P. gingivalis stimulation (p<0.01).
  
  
  CONCLUSIONS
  These findings provide evidence that L. rhamnosus ATCC9595 can modulate the inflammatory signals and could introduce P. gingivalis to immune systems by inducing CXCL8 secretion.
authors.affiliation: Department of Medical Microbiology, Faculty of Dentistry, Gazi University, Ankara, Turkey.
authors.forename: Ayşegül
authors.initials: A
authors.lastname: Mendi
authors.affiliation: PEDI-STEM Center for Stem Cell Research and Development, Hacettepe University, Ankara, Turkey.
authors.forename: Sevil
authors.initials: S
authors.lastname: Köse
authors.affiliation: PEDI-STEM Center for Stem Cell Research and Development, Hacettepe University, Ankara, Turkey.
authors.forename: Duygu
authors.initials: D
authors.lastname: Uçkan
authors.affiliation: Department of Medical Microbiology, Faculty of Dentistry, Gazi University, Ankara, Turkey.
authors.forename: Gülçin
authors.initials: G
authors.lastname: Akca
authors.affiliation: Department of Oral and Maxilofacial Surgery, Faculty of Dentistry, Gazi University, Ankara, Turkey.
authors.forename: Derviş
authors.initials: D
authors.lastname: Yilmaz
authors.affiliation: Department of Oral and Maxilofacial Surgery, Faculty of Dentistry, Gazi University, Ankara, Turkey.
authors.forename: Levent
authors.initials: L
authors.lastname: Aral
authors.affiliation: Department of Oral Pathology, Faculty of Dentistry, Gazi University, Ankara, Turkey.
authors.forename: Sibel Elif
authors.initials: SE
authors.lastname: Gültekin
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Başkent University, Ankara, Turkey.
authors.forename: Tamer
authors.initials: T
authors.lastname: Eroğlu
authors.affiliation: PEDI-STEM Center for Stem Cell Research and Development, Hacettepe University, Ankara, Turkey.
authors.forename: Emine
authors.initials: E
authors.lastname: Kiliç
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Başkent University, Ankara, Turkey.
authors.forename: Sina
authors.initials: S
authors.lastname: Uçkan
mesh_terms: D001422:Bacterial Adhesion / Q000276:immunology; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D007113:Immunity, Innate; D007371:Interferon-gamma / Q000032:analysis / Q000276:immunology; D016753:Interleukin-10; D016209:Interleukin-8 / Q000032:analysis* / Q000276:immunology; D052201:Lacticaseibacillus rhamnosus / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000382:microbiology*; D010518:Periodontitis / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000276:immunology*; D019936:Probiotics / Q000494:pharmacology*; D018709:Statistics, Nonparametric; D051197:Toll-Like Receptor 4 / Q000032:analysis / Q000276:immunology; D055815:Young Adult
chemical_list: C573415:IFNG protein, human; D016209:Interleukin-8; D051197:Toll-Like Receptor 4; D016753:Interleukin-10; D007371:Interferon-gamma