title: An epidermal growth factor receptor exon 19 mutation in a mucin-producing gastric cancer sample from a Chinese patient.
abstract: OBJECTIVE
  To determine whether a subgroup of gastric cancer patients might benefit from epidermal growth factor receptor-tyrosine kinase inhibitors.
  
  
  METHODS
  A total of 103 gastric cancer samples were collected for this study. High-resolution melting and deoxyribonucleic acid sequencing were used to detect epidermal growth factor receptor mutations in exons 19 and 21.
  
  
  RESULTS
  Polymerase chain reaction-high-resolution melting was successfully performed on all 103 samples. Aberrant melting curves were found in only one sample. Sanger sequencing revealed a 15 bp deletion (c.2235_2249del; p.Glu746_Ala750del) in epidermal growth factor receptor exon 19. The sample was from a male patient, and the pathological diagnosis was a mucin-producing gastric cancer with lymph node metastasis. To date, this is the first report on epidermal growth factor receptor exon 19 mutation in gastric cancer.
  
  
  CONCLUSIONS
  An epidermal growth factor receptor mutation in exon 19 was identified in mucin-producing gastric cancer sample from a male patient. This mutation indicates that the small subgroup of patients with mucin-producing gastric cancer might benefit from epidermal growth factor receptor-tyrosine kinase inhibitors.
authors.affiliation: Laboratory Center, The Second Affiliated Hospital of Dalian Medical University, Dalian Department of Pathology, Affiliated Zhongshan Hospital of Dalian University, Dalian.
authors.forename: Weipeng
authors.initials: W
authors.lastname: Lu
authors.affiliation: Laboratory Center, The Second Affiliated Hospital of Dalian Medical University, Dalian.
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Pathology, The Third People's Hospital of Dalian, Dalian.
authors.forename: Hai
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Dalian Medical University, Dalian, PR China.
authors.forename: Lina
authors.initials: L
authors.lastname: Liu
authors.affiliation: Laboratory Center, The Second Affiliated Hospital of Dalian Medical University, Dalian lvshen1956@126.com.
authors.forename: Shen
authors.initials: S
authors.lastname: Lu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics*; D002681:China; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D066246:ErbB Receptors / Q000235:genetics*; D005091:Exons; D005260:Female; D005752:Gastric Mucins / Q000378:metabolism*; D017353:Gene Deletion; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*; D044366:Transition Temperature
chemical_list: D004273:DNA, Neoplasm; D005752:Gastric Mucins; D066246:ErbB Receptors
keywords: EGFR; gastric cancer; gene mutation; tyrosine kinase inhibitors