title: Rapid screening and identification of lycodine-type alkaloids in Lycopodiaceae and Huperziaceae plants by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry.
abstract: Lycodine-type alkaloids have gained significant interest owing to their unique skeletal characteristics and acetylcholinesterase activity. This study established a rapid and reliable method using ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-ESI-Q/TOF-MS/MS) for comprehensive characterization of lycodine-type alkaloids for the first time. The lycodine-type alkaloids were detected successfully from Lycopodiastrum casuarinoides, Huperzia serrata and Phlegmarirus carinatus in seven plants of the Lycopodiaceae and Huperziaceae families, based on the established characteristic MS fragmentation of five known alkaloids. Furthermore, a total of 13 lycodine-type alkaloids were identified, of which three pairs of isomers were structurally characterized and differentiated. This study further improves mass analysis of lycodine-type alkaloids and demonstrates the superiority of UPLC with a high-resolution mass spectrometer for the rapid and sensitive structural elucidation of other trace active compounds. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Nanjing, People's Republic of China.
authors.forename: Si-Ming
authors.initials: SM
authors.lastname: Shan
authors.affiliation: Nanjing, People's Republic of China. luojg@cpu.edu.cn.
authors.forename: Jian-Guang
authors.initials: JG
authors.lastname: Luo
authors.affiliation: Nanjing, People's Republic of China.
authors.forename: Ke
authors.initials: K
authors.lastname: Pan
authors.affiliation: Nanjing, People's Republic of China.
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Zou
authors.affiliation: Nanjing, People's Republic of China. cpu_lykong@126.com.
authors.forename: Ling-Yi
authors.initials: LY
authors.lastname: Kong
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006576:Heterocyclic Compounds, 4 or More Rings / Q000032:analysis*; D032501:Huperzia / Q000737:chemistry; D029542:Lycopodiaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000470:Alkaloids; D006576:Heterocyclic Compounds, 4 or More Rings; D010936:Plant Extracts; C585001:lycodine
keywords: Huperziaceae; Lycopodiaceae; UPLC-ESI-Q/TOF-MS/MS; fragmentation patterns; lycodine-type alkaloids