title: Embryonic stem cell-derived neural progenitors transplanted to the hippocampus migrate on host vasculature.
abstract: This study describes the migration of transplanted ESNPs either injected directly into the hippocampus of a mouse, seeded onto hippocampal slices, or under in vitro culture conditions. We show that transplanted mouse ESNPs associate with, and appear to migrate on the surface of the vasculature, and that human ESNPs also associate with blood vessels when seeded on hippocampal slices, and migrate towards BECs in vitro using a Boyden chamber assay. This initial adhesion to vessels is mediated, at least in part, via the integrin α6β1, as observed for SVZ neural progenitor cells. Our data are consistent with CXCL12, expressed by the astroglial-vasculature niche, playing an important role in the migration of transplanted neural progenitors within and outside of the hippocampus.
authors.affiliation: Department of Biology, Wesleyan University, Middletown, CT, 06457, USA. Electronic address: classiter@wesleyan.edu.
authors.forename: Chelsea M
authors.initials: CM
authors.lastname: Lassiter
authors.affiliation: Department of Biology, Wesleyan University, Middletown, CT, 06457, USA.
authors.forename: Julian S
authors.initials: JS
authors.lastname: Gal
authors.affiliation: Department of Biology, Wesleyan University, Middletown, CT, 06457, USA.
authors.forename: Sandy
authors.initials: S
authors.lastname: Becker
authors.affiliation: School of Natural Sciences and Mathematics, Stockton University, Galloway, NJ, 08559, USA.
authors.forename: Nathaniel W
authors.initials: NW
authors.lastname: Hartman
authors.affiliation: Department of Biology, Wesleyan University, Middletown, CT, 06457, USA.
authors.forename: Laura
authors.initials: L
authors.lastname: Grabel
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism; D001808:Blood Vessels / Q000378:metabolism / Q000473:pathology; D002448:Cell Adhesion; D002454:Cell Differentiation; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000378:metabolism; D018920:Coculture Techniques; D053595:Embryonic Stem Cells / Q000166:cytology*; D042783:Endothelial Cells / Q000166:cytology; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D039121:Integrin alpha6beta1 / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology* / Q000637:transplantation
chemical_list: D054377:Chemokine CXCL12; D039121:Integrin alpha6beta1; D007797:Laminin