title: In Vivo study of naturally deformed Escherichia coli bacteria.
abstract: A combination of light-microscopy and image processing has been applied to study naturally deformed Escherichia coli under in vivo condition and at the order of sub-pixel high-resolution accuracy. To classify deflagellated non-dividing E. coli cells to the rod-shape and bent-shape, a geometrical approach has been applied. From the analysis of the geometrical data which were obtained of image processing, we estimated the required effective energy for shaping a rod-shape to a bent-shape with the same size. We evaluated the energy of deformation in the naturally deformed bacteria with minimum cell manipulation, under in vivo condition, and with minimum influence of any external force, torque and pressure. Finally, we have also elaborated on the possible scenario to explain how naturally deformed bacteria are formed from initial to final-stage.
authors.affiliation: Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Sharareh
authors.initials: S
authors.lastname: Tavaddod
authors.affiliation: Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. naderman@modares.ac.ir.
authors.forename: Hossein
authors.initials: H
authors.lastname: Naderi-Manesh
mesh_terms: D018407:Bacterial Physiological Phenomena; D001696:Biomechanical Phenomena; D004734:Energy Metabolism; D004926:Escherichia coli / Q000166:cytology* / Q000254:growth & development; D007091:Image Processing, Computer-Assisted; D008859:Microscopy, Polarization
keywords: Bacteria; Escherichia coli (E. coli); In vivo imaging; Membrane biophysics; Microscopic imaging