title: Capturing the ephemeral human pluripotent state.
abstract: During human development, pluripotency is present only in early stages of development. This ephemeral cell potential can be captured in vitro by obtaining pluripotent stem cells (PSC) with self-renewal properties, the human embryonic stem cells (hESC). However, diverse studies suggest the existence of a plethora of human PSC (hPSC) that can be derived from both embryonic and somatic sources, depending on defined culture conditions, their spatial origin, and the genetic engineering used for reprogramming. This review will focus on hPSC, covering the conventional primed hESC, naïve-like hPSC that resemble the ground-state of development, region-selective PSC, and human induced PSC (hiPSC). We will analyze differences and similarities in their differentiation potential as well as in the molecular circuitry of pluripotency. Finally, we describe the need for human feeder cells to derive and maintain hPSC, because they could emulate the interaction of in vivo pluripotent cells with extraembryonic structures that support development. Developmental Dynamics 245:762-773, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Departamento de Biología Celular, Instituto Nacional de Perinatología, México D.F., México.
authors.forename: Daniela
authors.initials: D
authors.lastname: Ávila-González
authors.affiliation: Departamento de Biología Celular, Instituto Nacional de Perinatología, México D.F., México.
authors.forename: Guadalupe
authors.initials: G
authors.lastname: García-López
authors.affiliation: Departamento de Biología Celular, Instituto Nacional de Perinatología, México D.F., México.
authors.forename: Irma Lydia
authors.initials: IL
authors.lastname: García-Castro
authors.affiliation: Departamento de Inmunobioquímica, Instituto Nacional de Perinatología, Lomas Virreyes, México D.F., México.
authors.forename: Héctor
authors.initials: H
authors.lastname: Flores-Herrera
authors.affiliation: Departamento de Biología Celular, Instituto Nacional de Perinatología, México D.F., México.
authors.forename: Anayansi
authors.initials: A
authors.lastname: Molina-Hernández
authors.affiliation: Departamento de Neurobiología Conductual y Cognitiva, Instituto de Neurobiología, Universidad Nacional Autónoma de México, Querétaro, México.
authors.forename: Wendy
authors.initials: W
authors.lastname: Portillo
authors.affiliation: Departamento de Biología Celular, Instituto Nacional de Perinatología, México D.F., México.
authors.forename: Néstor Fabián
authors.initials: NF
authors.lastname: Díaz
mesh_terms: D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D006801:Humans; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology
chemical_list: D050814:Octamer Transcription Factor-3
keywords: heterogeneity; naïve pluripotency; primed pluripotency