title: Sperm morphology of Muscidifurax uniraptor (Hymenoptera: Chalcidoidea: Pteromalidae).
abstract: Sperm morphology of the parasitoid Muscidifurax uniraptor was investigated under light and transmission electron microscopy. M. uniraptor sperm are filiform, spiraled, approximately 150 μm in length, with a distinctive head, hooded by an extracellular sheath and a flagellum. This extracellular layer, from which many filaments radiate, measures approximately 90 nm in thickness and covers a small acrosome and the anterior nuclear region. The acrosome is composed of an acrosomal vesicle and a perforatorium with its base inserted in the nuclear tip. The nucleus is filled with homogeneously compacted chromatin. The centriolar adjunct extends towards the anterior portion in a spiral around the nucleus for 3.5 μm in length. The two mitochondrial derivatives begin exactly at the centriole adjunct base and, in cross-section, have a circular shape with equal areas that are smaller than the axoneme diameter. It is coiled, with 9 + 9 + 2 microtubules and begins from the centriole, just below the nuclear base. The axoneme is connected to the mitochondrial derivatives by two small irregularly shaped masses. Between the derivatives and the axoneme, the 'center-flagellar material' is observed. Overall, these characteristics are recognized in other Chalcidoidea, especially in the eurytomids, but together they form a set of species-specific data.
authors.affiliation: Departamento de Biologia Geral, Universidade Federal de Viçosa-UFV, Minas Gerais, Brazil.
authors.forename: Helen Pinto
authors.initials: HP
authors.lastname: Santos
authors.affiliation: Departamento de Biologia Geral, Universidade Federal de Viçosa-UFV, Minas Gerais, Brazil.
authors.forename: Marcelo Silva
authors.initials: MS
authors.lastname: Barcellos
authors.affiliation: Departamento de Biologia Geral, Universidade Federal de Viçosa-UFV, Minas Gerais, Brazil.
authors.forename: Aline Beatriz
authors.initials: AB
authors.lastname: Reis
authors.affiliation: Departamento de Biologia Celular, Universidade Estadual de Campinas-UNICAMP, São Paulo, Brazil.
authors.forename: Heidi
authors.initials: H
authors.lastname: Dolder
authors.affiliation: Departamento de Biologia Geral, Universidade Federal de Viçosa-UFV, Minas Gerais, Brazil. Electronic address: linoneto@ufv.br.
authors.forename: José
authors.initials: J
authors.lastname: Lino-Neto
mesh_terms: D000818:Animals; D022083:Cytoplasmic Structures / Q000648:ultrastructure; D008297:Male; D046529:Microscopy, Electron, Transmission; D013045:Species Specificity; D013094:Spermatozoa / Q000648:ultrastructure*; D014863:Wasps / Q000166:cytology / Q000648:ultrastructure*
keywords: Centriolar adjunct; Parasitoid; Spermatozoa; Spiraled sperm; Ultrastructure