title: Efficient Purification and Release of Circulating Tumor Cells by Synergistic Effect of Biomarker and SiO2 @Gel-Microbead-Based Size Difference Amplification.
abstract: Microfluidics-based circulating tumor cell (CTC) isolation is achieved by using gelatin-coated silica microbeads conjugated to CTC-specific antibodies. Bead-binding selectively enlarges target cell size, providing efficient high-purity capture. CTCs captured can be further released non-invasively. This stratagem enables high-performance CTC isolation for subsequent studies.
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education School of Physics and Technology, Wuhan University, Wuhan, 430072, Hubei, P. R. China.
authors.forename: Qinqin
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education School of Physics and Technology, Wuhan University, Wuhan, 430072, Hubei, P. R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Cai
authors.affiliation: Institute for Interdisciplinary Research, Jianghan University, Wuhan, 430056, China.
authors.forename: Bolei
authors.initials: B
authors.lastname: Chen
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education School of Physics and Technology, Wuhan University, Wuhan, 430072, Hubei, P. R. China.
authors.forename: Lang
authors.initials: L
authors.lastname: Rao
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education School of Physics and Technology, Wuhan University, Wuhan, 430072, Hubei, P. R. China.
authors.forename: Zhaobo
authors.initials: Z
authors.lastname: He
authors.affiliation: Institute for Interdisciplinary Research, Jianghan University, Wuhan, 430056, China.
authors.forename: Rongxiang
authors.initials: R
authors.lastname: He
authors.affiliation: Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA, 16802, USA.
authors.forename: Feng
authors.initials: F
authors.lastname: Guo
authors.affiliation: Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry Chinese Academy of Science, Beiyi Street 2#, Zhongguancun, Beijing, 100190, P. R. China.
authors.forename: Libo
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education School of Physics and Technology, Wuhan University, Wuhan, 430072, Hubei, P. R. China.
authors.forename: Kiran Kumar
authors.initials: KK
authors.lastname: Kondamareddy
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education School of Physics and Technology, Wuhan University, Wuhan, 430072, Hubei, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education School of Physics and Technology, Wuhan University, Wuhan, 430072, Hubei, P. R. China.
authors.forename: Shishang
authors.initials: S
authors.lastname: Guo
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education School of Physics and Technology, Wuhan University, Wuhan, 430072, Hubei, P. R. China.
authors.forename: Xing-Zhong
authors.initials: XZ
authors.lastname: Zhao
mesh_terms: D000912:Antibodies, Neoplasm / Q000737:chemistry*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D048429:Cell Size; D005780:Gelatin / Q000737:chemistry; D006801:Humans; D056656:Lab-On-A-Chip Devices; D061986:MCF-7 Cells; D008863:Microspheres; D009360:Neoplastic Cells, Circulating / Q000378:metabolism* / Q000473:pathology; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000912:Antibodies, Neoplasm; D014408:Biomarkers, Tumor; D012822:Silicon Dioxide; D005780:Gelatin
keywords: cell capture; cell release; circulating tumor cells; microfluidics; size amplification