title: Effects of Helicobacter pylori Infection on the Expressions and Functional Activities of Human Duodenal Mucosal Bicarbonate Transport Proteins.
abstract: BACKGROUND
  The mechanisms for Helicobacter pylori (H. pylori)-induced duodenal ulcerogenesis are not fully understood. In this study, we investigated the effects of H. pylori infection on the expressions and functional activities of human duodenal mucosal bicarbonate transport proteins and hope to further clarify the pathogenesis of H. pylori-associated duodenal ulcer.
  
  
  METHODS
  The experiments were performed in the patients with H. pylori-associated duodenal ulcers, H. pylori-associated chronic gastritis, and H. pylori-negative healthy subjects. Duodenal mucosal bicarbonate secretion was measured by Ussing Chamber technology.
  
  
  RESULTS
  The expressions of duodenal mucosal bicarbonate transport proteins, CFTR (cystic fibrosis transmembrane conductance regulator) and SLC26A6 (solute-linked carrier 26 gene A6), in the patients with H. pylori-associated duodenal ulcers were markedly lower than those in healthy controls. Basal and both forskolin- and prostaglandin E2-stimulated duodenal mucosal bicarbonate secretions in the patients with H. pylori-associated duodenal ulcers were also lower than those in healthy controls. After anti-H. pylori treatment for H. pylori-associated duodenal ulcers, duodenal mucosal bicarbonate secretion and CFTR and SLC26A6 expressions in H. pylori-eradicated patients recovered to levels comparable to healthy controls, but those were found to be not significantly altered in non-H. pylori-eradicated patients. The further results showed that decreases in the H. pylori-induced CFTR and SLC26A6 expression were related to the severity and virulent factors of H. pylori infection.
  
  
  CONCLUSIONS
  H. pylori infection impairs the expressions and functional activities of duodenal mucosal bicarbonate transport proteins, CFTR and SLC26A6, which contributes to the development of duodenal ulcer.
authors.affiliation: Department of Gastroenterology, Affiliated Hospital, Zunyi Medical College, Zunyi, China.|Digestive Disease Institute of Guizhou Province, Zunyi, China.|Research Center of Medicine and Biology, Zunyi Medical College, Zunyi, China.
authors.forename: Guorong
authors.initials: G
authors.lastname: Wen
authors.affiliation: Department of Gastroenterology, Affiliated Hospital, Zunyi Medical College, Zunyi, China.|Digestive Disease Institute of Guizhou Province, Zunyi, China.|Research Center of Medicine and Biology, Zunyi Medical College, Zunyi, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Jin
authors.affiliation: Department of Gastroenterology, Affiliated Hospital, Zunyi Medical College, Zunyi, China.|Digestive Disease Institute of Guizhou Province, Zunyi, China.
authors.forename: Shili
authors.initials: S
authors.lastname: Deng
authors.affiliation: Department of Gastroenterology, Affiliated Hospital, Zunyi Medical College, Zunyi, China.|Digestive Disease Institute of Guizhou Province, Zunyi, China.|Research Center of Medicine and Biology, Zunyi Medical College, Zunyi, China.
authors.forename: Jingyu
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Gastroenterology, Affiliated Hospital, Zunyi Medical College, Zunyi, China.|Digestive Disease Institute of Guizhou Province, Zunyi, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology, Affiliated Hospital, Zunyi Medical College, Zunyi, China.|Digestive Disease Institute of Guizhou Province, Zunyi, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xie
authors.affiliation: Department of Gastroenterology, Affiliated Hospital, Zunyi Medical College, Zunyi, China.|Digestive Disease Institute of Guizhou Province, Zunyi, China.|Research Center of Medicine and Biology, Zunyi Medical College, Zunyi, China.
authors.forename: Biguang
authors.initials: B
authors.lastname: Tuo
mesh_terms: D000328:Adult; D001639:Bicarbonates / Q000378:metabolism*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000378:metabolism*; D004386:Duodenum / Q000473:pathology*; D005260:Female; D016481:Helicobacter Infections / Q000473:pathology*; D006801:Humans; D007413:Intestinal Mucosa / Q000473:pathology*; D008297:Male; D026901:Membrane Transport Proteins / Q000378:metabolism*; D000076988:Sulfate Transporters; D055815:Young Adult
chemical_list: D001639:Bicarbonates; C505032:CFTR protein, human; D026901:Membrane Transport Proteins; C439529:SLC26A6 protein, human; D000076988:Sulfate Transporters; D019005:Cystic Fibrosis Transmembrane Conductance Regulator
keywords: 
  CFTR
  ; 
  Helicobacter pylori
  ; Duodenum; SLC26A6; bicarbonate secretion