title: Conserved transmembrane glycine residues in the Shigella flexneri polysaccharide co-polymerase protein WzzB influence protein-protein interactions.
abstract: The O antigen (Oag) component of lipopolysaccharides (LPS) is crucial for virulence and Oag chain-length regulation is controlled by the polysaccharide co-polymerase class 1 (PCP1) proteins. Crystal structure analyses indicate that structural conservation among PCP1 proteins is highly maintained, however the mechanism of Oag modal-chain-length control remains to be fully elucidated. Shigella flexneri PCP1 protein WzzBSF confers a modal-chain length of 10-17 Oag repeat units (RUs), whereas the Salmonella enterica Typhimurium PCP1 protein WzzBST confers a modal-chain length of ~16-28 Oag RUs. Both proteins share >70 % overall sequence identity and contain two transmembrane (TM1 and TM2) regions, whereby a conserved proline-glycine-rich motif overlapping the TM2 region is identical in both proteins. Conserved glycine residues within TM2 are functionally important, as glycine to alanine substitutions at positions 305 and 311 confer very short Oag modal-chain length (~2-6 Oag RUs). In this study, WzzBSF was co-expressed with WzzBST in S. flexneri and a single intermediate modal-chain length of ~11-21 Oag RUs was observed, suggesting the presence of Wzz:Wzz interactions. Interestingly, co-expression of WzzBSF with WzzBG305A/G311A conferred a bimodal LPS Oag chain length (despite over 99 % protein sequence identity), and we hypothesized that the proteins fail to interact. Co-purification assays detected His6-WzzBSF co-purifying with FLAG-tagged WzzBST but not with FLAG-tagged WzzBG305A/G311A, supporting our hypothesis. These data indicate that the conserved glycine residues in TM2 are involved in Wzz:Wzz interactions, and provide insight into key interactions that drive Oag modal length control.
authors.affiliation: School of Biological Sciences, Department of Molecular & Cellular Biology, Research Centre for Infectious Diseases, University of Adelaide, Adelaide 5005, Australia.
authors.forename: Magdalene
authors.initials: M
authors.lastname: Papadopoulos
authors.affiliation: School of Biological Sciences, Department of Molecular & Cellular Biology, Research Centre for Infectious Diseases, University of Adelaide, Adelaide 5005, Australia.
authors.forename: Elizabeth Ngoc Hoa
authors.initials: ENH
authors.lastname: Tran
authors.affiliation: School of Biological Sciences, Department of Molecular & Cellular Biology, Research Centre for Infectious Diseases, University of Adelaide, Adelaide 5005, Australia.
authors.forename: Gerald Laurence
authors.initials: GL
authors.lastname: Murray
authors.affiliation: School of Biological Sciences, Department of Molecular & Cellular Biology, Research Centre for Infectious Diseases, University of Adelaide, Adelaide 5005, Australia.
authors.forename: Renato
authors.initials: R
authors.lastname: Morona
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D018360:Crystallography, X-Ray; D015964:Gene Expression Regulation, Bacterial; D005998:Glycine / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000737:chemistry; D019081:O Antigens / Q000096:biosynthesis*; D000072417:Protein Domains / Q000235:genetics*; D012486:Salmonella typhimurium / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D012763:Shigella flexneri / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D008070:Lipopolysaccharides; D019081:O Antigens; C075969:rol protein, Bacteria; D005998:Glycine