title: Epizootiology of Perkinsus sp. inCrassostrea gasar oysters in polyculture with shrimps in northeastern Brazil.
abstract: Bivalve culture is of considerable economic and social interest in northeastern (NE) Brazil. The polyculture is an alternative approach to traditional monoculture for reducing the environmental impact of shrimp farming and improving oyster culture. Perkinsus marinus and Perkinsus olseni were found infecting oysters in NE Brazil and can threaten oyster production. This study evaluated Perkinsus spp. occurrence in Crassostrea gasar during all production stages. Oyster spats were produced in a hatchery and grown in shrimp ponds in Rio Grande do Norte state. Perkinsus spp. were surveyed by Ray's fluid thioglycollate medium and confirmed by polymerase chain reaction. Prevalence and intensity of infection were determined in oysters until they reached 7 cm. Results showed that the broodstock was already infected by Perkinsus (60%), but the derived spats were Perkinsus-free. Oyster spats acquired Perkinsus infection when transferred to ponds. The prevalence gradually increased in the seven months following placement in ponds (73%), and then decreased to 17% by the tenth month. The infections were initially mild, but intensity increased at the final growth stage. In conclusion, it is possible to produce Perkinsus-free C. gasar oyster spats from infected broodstock, and their culture in shrimp ponds is feasible.
authors.affiliation: Centro de Ciências Exatas e da Natureza, Universidade Federal da Paraíba, João Pessoa, PB, Brasil.
authors.forename: Patricia Mirella
authors.initials: PM
authors.lastname: da Silva
authors.affiliation: Centro de Ciências Exatas e da Natureza, Universidade Federal da Paraíba, João Pessoa, PB, Brasil.
authors.forename: Carolina Pereira
authors.initials: CP
authors.lastname: Costa
authors.affiliation: Centro de Ciências Exatas e da Natureza, Universidade Federal da Paraíba, João Pessoa, PB, Brasil.
authors.forename: Jaíse Paiva Bragante
authors.initials: JP
authors.lastname: de Araújo
authors.affiliation: Centro de Ciências Exatas e da Natureza, Universidade Federal da Paraíba, João Pessoa, PB, Brasil.
authors.forename: Fernando Ramos
authors.initials: FR
authors.lastname: Queiroga
authors.affiliation: Fazenda PRIMAR, Tibau do Sul, RN, Brasil.
authors.forename: Alexandre Alter
authors.initials: AA
authors.lastname: Wainberg
mesh_terms: D000818:Animals; D016782:Apicomplexa; D017756:Aquaculture / Q000379:methods*; D001938:Brazil; D049896:Crassostrea / Q000254:growth & development / Q000469:parasitology*; D003445:Crustacea / Q000254:growth & development; D016133:Polymerase Chain Reaction; D011529:Protozoan Infections, Animal / Q000453:epidemiology*