title: Regulatory effect of Bcl-2 in ultraviolet radiation-induced apoptosis of the mouse crystalline lens.
abstract: The aim of the present study was to analyze the role of Bcl-2 during the process of apoptosis in the mouse crystalline lens. In total, 12 normal mice served as the control group and 12 Bcl-2 knockout (K.O) mice served as the experimental group. The mouse crystalline lens was sampled for the detection of Bcl-2 and caspase-3 expression following exposure to ultraviolet (UV) radiation. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to determine Bcl-2 expression in the groups of normal mice receiving UV radiation or not receiving UV radiation. Samples of the murine crystalline lens were microscopically harvested and analyzed using western blotting. Apoptosis was detected using terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay. Furthermore, caspase 3 activity was examined using enzyme-linked immunosorbent assay kits, and RT-qPCR was used to analyze caspase-3 expression levels. The results of the present study demonstrated that there was no statistically significant difference in the level of Bcl-2 gene transcription between the two groups. In addition, UV radiation did not change the macrostructure of the crystalline lens in the group of normal mice or the group of Bcl-2 K.O mice. The results of the TUNEL assay indicated that the normal-UV group exhibited a more significant apoptosis level compared with the Bcl-2 K.O-UV group. Furthermore, the mRNA expression level of caspase-3 in the normal-UV group was significantly higher compared with the normal-nonUV group (P<0.05), while the levels in the Bcl-2 K.O-UV group were significantly higher compared with the Bcl-2 K.O and normal-nonUV groups (P<0.05). In addition, the mRNA expression level of caspase-3 was significantly higher in the normal-UV, as compared with the Bcl-2 K.O-UV group (P<0.05), and the variation trends in caspase-3 activity were consistent. In conclusion, the results of the present study demonstrated that Bcl-2 may have an important role in the promotion of UV-induced apoptosis in the crystalline lens.
authors.affiliation: Department of Ophthalmology, Eye Hospital of The Second Clinical Hospital of Jilin University, Jilin University, Changchun, Jilin 130051, P.R. China.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Department of Ophthalmology, Eye Hospital of The Second Clinical Hospital of Jilin University, Jilin University, Changchun, Jilin 130051, P.R. China.
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Ophthalmology, Eye Hospital of The Second Clinical Hospital of Jilin University, Jilin University, Changchun, Jilin 130051, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Department of Ophthalmology, Eye Hospital of The Second Clinical Hospital of Jilin University, Jilin University, Changchun, Jilin 130051, P.R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Department of Ophthalmology, Eye Hospital of The Second Clinical Hospital of Jilin University, Jilin University, Changchun, Jilin 130051, P.R. China.
authors.forename: Meisheng
authors.initials: M
authors.lastname: Zhao
keywords: Bcl-2; apoptosis; crystalline lens; ultraviolet