title: Early LPS-induced ERK activation in retinal pigment epithelium cells is dependent on PIP 2 -PLC.
abstract: This article presents additional data regarding the study "The phospholipase D pathway mediates the inflammatory response of the retinal pigment epithelium" [1]. The new data presented here show that short exposure of RPE cells to lipopolysaccharide (LPS) induces an early and transient activation of the extracellular signal-regulated kinase (ERK1/2). This early ERK1/2 activation is dependent on phosphatidylinositol bisphosphate-phospholipase C (PIP2-PLC). On the contrary, neither the phospholipase D 1 (PLD1) nor the PLD2 inhibition is able to modulate the early ERK1/2 activation induced by LPS in RPE cells.
authors.affiliation: Instituto de Investigaciones Bioquímicas de Bahía Blanca (INIBIBB), Universidad Nacional del Sur (UNS) and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Camino La Carrindanga km 7, 8000 Bahía Blanca, Argentina.
authors.forename: Melina V
authors.initials: MV
authors.lastname: Mateos
authors.affiliation: Instituto de Investigaciones Bioquímicas de Bahía Blanca (INIBIBB), Universidad Nacional del Sur (UNS) and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Camino La Carrindanga km 7, 8000 Bahía Blanca, Argentina.
authors.forename: Constanza B
authors.initials: CB
authors.lastname: Kamerbeek
authors.affiliation: Instituto de Investigaciones Bioquímicas de Bahía Blanca (INIBIBB), Universidad Nacional del Sur (UNS) and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Camino La Carrindanga km 7, 8000 Bahía Blanca, Argentina.
authors.forename: Norma M
authors.initials: NM
authors.lastname: Giusto
authors.affiliation: Instituto de Investigaciones Bioquímicas de Bahía Blanca (INIBIBB), Universidad Nacional del Sur (UNS) and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Camino La Carrindanga km 7, 8000 Bahía Blanca, Argentina.
authors.forename: Gabriela A
authors.initials: GA
authors.lastname: Salvador
keywords: ERK, extracellular signal-regulated kinase; Extracellular signal-regulated kinase (ERK1/2); HRP, horseradish peroxidase; Inflammation; LPS, lipopolysaccharide; Lipopolysaccharide (LPS); PIP2-PLC, phosphatidylinositol bisphosphate-phospholipase C; PLD, phospholipase D; Phosphatidylinositol bisphosphate-phospholipase C (PIP2-PLC); Phospholipase D (PLD); RPE, retinal pigment epithelium; Retinal pigment epithelium (RPE)