title: Abnormal miRNA-30e Expression is Associated with Breast Cancer Progression.
abstract: BACKGROUND
  microRNAs (miRNAs) are involved in the regulation of various cellular processes, such as differentiation, proliferation, metabolism, and apoptosis, and they have been implicated in several diseases, including cancers.
  
  
  METHODS
  To assess the role of miRNA in the progression of breast cancer, we performed TaqMan-based miRNA profiling for plasma from patients with breast cancer (n = 53), unrelated diseases (n = 40), or matched healthy controls (n = 40), and for breast tumors or adjacent non-tumors (n = 41).
  
  
  RESULTS
  We selected 18 miRNAs with predicted roles in breast cancer and demonstrated that let-7i (p = 0.019), let-7a (p = 0.02), and miR-650 (p = 0.008) were significantly up-regulated in plasma; miR-21 (p < 0.001) is up-regulated in breast cancer tissue, and miR-30e was down-regulated in both plasma (p < 0.001) and breast cancer tissues (p = 0.004). Plasma miR-30e expression was shown to be statistically associated with age (p = 0.0402) and clinical stage (p = 0.007). However, receiver-operating characteristic curve analyses suggested that miR-30e expression cannot significantly differentiate breast cancer from healthy tissue or plasma. Consistent with a potential role for miR-30e in breast cancer, three predicted targets of miR-30e (RAB11A, BNIP3L, and RAB32) are up-regulated in breast cancer tissue.
  
  
  CONCLUSIONS
  These findings suggest that reduced miR-30e correlates with the clinical stage of breast cancer. It is worthwhile to further explore that the potential role of miR-30e as a tumor suppressor in breast cancer, as well as its potential therapeutic utility.
authors.forename: Ziying
authors.initials: Z
authors.lastname: Lin
authors.forename: Jian-wen
authors.initials: JW
authors.lastname: Li
authors.forename: Yahong
authors.initials: Y
authors.lastname: Wang
authors.forename: Ting
authors.initials: T
authors.lastname: Chen
authors.forename: Nina
authors.initials: N
authors.lastname: Ren
authors.forename: Lawei
authors.initials: L
authors.lastname: Yang
authors.forename: Wenya
authors.initials: W
authors.lastname: Xu
authors.forename: Huijuan
authors.initials: H
authors.lastname: He
authors.forename: Yun
authors.initials: Y
authors.lastname: Jiang
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Chen
authors.forename: Tie
authors.initials: T
authors.lastname: Liu
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
mesh_terms: D000328:Adult; D000367:Age Factors; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D018450:Disease Progression; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D011518:Proto-Oncogene Proteins / Q000235:genetics; D012372:ROC Curve; D012307:Risk Factors; D025521:Tumor Suppressor Proteins / Q000235:genetics; D055815:Young Adult; D020691:rab GTP-Binding Proteins / Q000235:genetics; D000099193:rab11 GTP-Binding Proteins
chemical_list: D014408:Biomarkers, Tumor; D008565:Membrane Proteins; D035683:MicroRNAs; D011518:Proto-Oncogene Proteins; D025521:Tumor Suppressor Proteins; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins; C116838:BNIP3L protein, human; C546086:MIRN30b microRNA, human; C466094:Rab32 protein, human