title: Antimicrobial resistance in faecal Escherichia coli isolates from farmed red deer and wild small mammals. Detection of a multiresistant E. coli producing extended-spectrum beta-lactamase.
abstract: Eighty-nine Escherichia coli isolates recovered from faeces of red deer and small mammals, cohabiting the same area, were analyzed to determine the prevalence and mechanisms of antimicrobial resistance and molecular typing. Antimicrobial resistance was detected in 6.7% of isolates, with resistances to tetracycline and quinolones being the most common. An E. coli strain carrying blaCTX-M-1 as well as other antibiotic resistant genes included in an unusual class 1 integron (Intl1-dfrA16-blaPSE-1-aadA2-cmlA1-aadA1-qacH-IS440-sul3-orf1-mef(B)Δ-IS26) was isolated from a deer. The blaCTX-M-1 gene was transferred by conjugation and transconjugants also acquired an IncN plasmid. This strain was typed as ST224, which seems to be well adapted to both clinical and environmental settings. The phylogenetic distribution of the 89 strains varied depending on the animal host. This work reveals low antimicrobial resistance levels among faecal E. coli from wild mammals, which reflects a lower selective pressure affecting these bacteria, compared to livestock. However, it is remarkable the detection of a multi-resistant ESBL-E. coli with an integron carrying clinically relevant antibiotic-resistance genes, which can contribute to the dissemination of resistance determinants among different ecosystems.
authors.affiliation: Área Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain.
authors.forename: C A
authors.initials: CA
authors.lastname: Alonso
authors.affiliation: Health & Biotechnology (SaBio) Group, Spanish Wildlife Research Institute IREC (CSIC-UCLM-JCCM), Ciudad Real, Spain.
authors.forename: D
authors.initials: D
authors.lastname: González-Barrio
authors.affiliation: Área Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Tenorio
authors.affiliation: Health & Biotechnology (SaBio) Group, Spanish Wildlife Research Institute IREC (CSIC-UCLM-JCCM), Ciudad Real, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Ruiz-Fons
authors.affiliation: Área Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain. Electronic address: carmen.torres@unirioja.es.
authors.forename: C
authors.initials: C
authors.lastname: Torres
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000382:microbiology; D000835:Animals, Wild / Q000382:microbiology*; D000890:Anti-Infective Agents / Q000494:pharmacology; D003670:Deer / Q000382:microbiology*; D018432:Drug Resistance, Multiple; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D005243:Feces / Q000382:microbiology*; D032023:Integrons; D008826:Microbial Sensitivity Tests; D058889:Molecular Typing; D010802:Phylogeny; D015995:Prevalence; D012377:Rodentia / Q000382:microbiology; D013030:Spain / Q000453:epidemiology; D001618:beta-Lactamases / Q000096:biosynthesis*
chemical_list: D000890:Anti-Infective Agents; D001618:beta-Lactamases
keywords: Antimicrobial resistance; Deer; ESBL; Escherichia coli; Small mammals; Wildlife