title: Improved Performance of Lipase Immobilized on Tannic Acid-Templated Mesoporous Silica Nanoparticles.
abstract: Mesoporous silica nanoparticles were synthesized by using tannic acid as a pore-forming agent, which is an environmentally friendly, cheap, and non-surfactant template. SEM and TEM images indicated that the tannic acid-templated mesoporous silica nanoparticles (TA-MSNs) are monodisperse spherical-like particles with an average diameter of 195 ± 16 nm. The Brunauer-Emmett-Teller (BET) results showed that the TA-MSNs had a relatively high surface area (447 m(2)/g) and large pore volume (0.91 cm(3)/g), and the mean pore size was ca. 10.1 nm. Burkholderia cepacia lipase was immobilized on the TA-MSNs by physical adsorption for the first time, and the properties of immobilized lipase (BCL@TA-MSNs) were investigated. The BCL@TA-MSNs exhibited satisfactory thermal stability; strong tolerance to organic solvents such as methanol, ethanol, isooctane, n-hexane, and tetrahydrofuran; and high operational reusability when BCL@TA-MSNs were applied in esterification and transesterification reactions. After recycling 15 times in the transesterification reaction for biodiesel production, over 85 % of biodiesel yield can be maintained. With these desired characteristics, the TA-MSNs may provide excellent candidates for enzyme immobilization.
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin, 300130, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin, 300130, China.
authors.forename: Wenya
authors.initials: W
authors.lastname: Sun
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin, 300130, China.
authors.forename: Liya
authors.initials: L
authors.lastname: Zhou
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin, 300130, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin, 300130, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: He
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin, 300130, China. jgao@hebut.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
mesh_terms: D056804:Biofuels; D016956:Burkholderia cepacia / Q000201:enzymology; D004800:Enzymes, Immobilized / Q000096:biosynthesis / Q000737:chemistry*; D004951:Esterification; D008049:Lipase / Q000096:biosynthesis / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry; D013634:Tannins / Q000737:chemistry*
chemical_list: D056804:Biofuels; D004800:Enzymes, Immobilized; D013634:Tannins; D012822:Silicon Dioxide; D008049:Lipase; D000432:Methanol
keywords: Biodiesel; Immobilized enzyme; Mesoporous silica particles; Tannic acid