title: The impact of intrinsic ageing on the protein composition of the dermal-epidermal junction.
abstract: The dermal-epidermal junction of human skin exhibits age-related remodelling, resulting in a flattened appearance and reduced surface area. Despite this, a paucity of information is available regarding which protein components change with advancing age. Here we report a significant reduction in the protein distribution of collagen IV (P<0.0001), collagen VII (P<0.001), collagen XVII (P<0.01), integrin β4 (P<0.001) and laminin-332 (P<0.0001) in intrinsically aged skin. The functional implication of this altered protein composition appears to be loss of structural integrity and may, in part, explain the increased fragility of aged skin.
authors.affiliation: Centre for Dermatology Research, Institute of Inflammation & Repair, Manchester Academic Health Science Centre, The University of Manchester, UK; The Dermatology Centre, Salford Royal NHS Foundation Trust, Salford, UK.
authors.forename: Abigail K
authors.initials: AK
authors.lastname: Langton
authors.affiliation: Centre for Dermatology Research, Institute of Inflammation & Repair, Manchester Academic Health Science Centre, The University of Manchester, UK; The Dermatology Centre, Salford Royal NHS Foundation Trust, Salford, UK.
authors.forename: Poonam
authors.initials: P
authors.lastname: Halai
authors.affiliation: Centre for Dermatology Research, Institute of Inflammation & Repair, Manchester Academic Health Science Centre, The University of Manchester, UK; The Dermatology Centre, Salford Royal NHS Foundation Trust, Salford, UK.
authors.forename: Christopher E M
authors.initials: CE
authors.lastname: Griffiths
authors.affiliation: Centre for Tissue Injury and Repair, Institute of Inflammation & Repair, Manchester Academic Health Science Centre, The University of Manchester, UK.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Sherratt
authors.affiliation: Centre for Dermatology Research, Institute of Inflammation & Repair, Manchester Academic Health Science Centre, The University of Manchester, UK; The Dermatology Centre, Salford Royal NHS Foundation Trust, Salford, UK. Electronic address: rachel.watson@manchester.ac.uk.
authors.forename: Rachel E B
authors.initials: RE
authors.lastname: Watson
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D003094:Collagen / Q000378:metabolism*; D020405:Dermis / Q000378:metabolism* / Q000473:pathology; D004817:Epidermis / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D039663:Integrin beta4 / Q000378:metabolism*; D007797:Laminin / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D015595:Skin Aging
chemical_list: C059834:ITGB4 protein, human; D039663:Integrin beta4; D007797:Laminin; D003094:Collagen
keywords: Dermal-epidermal junction; Intrinsic ageing