title: Profiling of urinary amino-carboxylic metabolites by in-situ heptafluorobutyl chloroformate mediated sample preparation and gas chromatography-mass spectrometry.
abstract: A novel 1,1,1,2,2,3,3-heptafluorobutyl chloroformate reagent (HFBCF) was examined for in-situ derivatization of amino-carboxylic metabolites in human urine. The arising reaction products exhibit greatly reduced polarity which facilitates combining the derivatization and liquid-liquid microextraction (LLME) from an aqueous urine into an isooctane phase and immediate gas chromatographic-mas spectrometric analysis (GC-MS). The sample preparation protocol is simple, proceeds without an alcohol excess and provides cleaner extracts than other urinary GC-MS based methods. Moreover, thiol metabolites bound in disulfide bonds can be released by reduction with tris(3-hydroxypropyl)phosphine (THP) prior to the developed derivatization and LLME step. In order to evaluate potential of the novel method for GC-MS metabolomics, reaction products of 153 urinary metabolites with HFBCF, particularly those possessing amino and carboxyl groups (56 amino acids and their conjugates, 84 organic acids, 9 biogenic amines, 4 other polar analytes) and two internal standards were investigated in detail by GC-MS and liquid chromatography-mass spectrometry (LC-MS). One hundred and twenty metabolites (78%) yielded a single product, 25 (16%) and 2 metabolites (2-methylcitrate, citrate) generated two and more derivatives. From the examined set, analytically applicable products of 5 metabolites were not detected; the derivatives of 3 metabolites were only suitable for LC-MS analysis. Electron ionization (EI) of the examined analytes contained characteristic, diagnostic ions enabling to distinguish related and isomeric structures. The new method was validated for 132 metabolites using two internal standards in artificial urine and with special attention to potential disease biomarker candidates. The developed sample preparation protocol was finally evaluated by means of a certified organic acid standard mixture in urine and by GC-MS analysis of 100 morning urines obtained from healthy patients (50 males and 50 females), where 112 physiological metabolites were quantified in a 25 μL sample aliquot. The quantification data for the set were satisfactory, most metabolites were found within the range reported in the reference human metabolome (HMDB) database and literature. The reported results suggest that the described method has been a novel promising tool for targeted GC-MS based metabolomic analysis in urine.
authors.affiliation: Faculty Hospital Ostrava, Institute of Laboratory Diagnostics, Department of Biochemistry, 17. listopadu 1790, 70852 Ostrava, Czech Republic; Biology Centre, Czech Academy of Sciences, Institute of Entomology, Analytical Biochemistry and Metabolomics, Branišovská 31, 370 05 České Budějovice, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Hušek
authors.affiliation: Faculty Hospital Ostrava, Institute of Laboratory Diagnostics, Department of Biochemistry, 17. listopadu 1790, 70852 Ostrava, Czech Republic.
authors.forename: Zdeněk
authors.initials: Z
authors.lastname: Švagera
authors.affiliation: Faculty Hospital Ostrava, Institute of Laboratory Diagnostics, Department of Biochemistry, 17. listopadu 1790, 70852 Ostrava, Czech Republic.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Hanzlíková
authors.affiliation: Biology Centre, Czech Academy of Sciences, Institute of Entomology, Analytical Biochemistry and Metabolomics, Branišovská 31, 370 05 České Budějovice, Czech Republic.
authors.forename: Lucie
authors.initials: L
authors.lastname: Řimnáčová
authors.affiliation: Biology Centre, Czech Academy of Sciences, Institute of Entomology, Analytical Biochemistry and Metabolomics, Branišovská 31, 370 05 České Budějovice, Czech Republic.
authors.forename: Helena
authors.initials: H
authors.lastname: Zahradníčková
authors.affiliation: Biology Centre, Czech Academy of Sciences, Institute of Entomology, Analytical Biochemistry and Metabolomics, Branišovská 31, 370 05 České Budějovice, Czech Republic.
authors.forename: Iva
authors.initials: I
authors.lastname: Opekarová
authors.affiliation: Biology Centre, Czech Academy of Sciences, Institute of Entomology, Analytical Biochemistry and Metabolomics, Branišovská 31, 370 05 České Budějovice, Czech Republic. Electronic address: simek.bc@gmail.com.
authors.forename: Petr
authors.initials: P
authors.lastname: Šimek
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D002264:Carboxylic Acids / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005260:Female; D005466:Fluorocarbons / Q000737:chemistry*; D005561:Formates / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007202:Indicators and Reagents; D059627:Liquid Phase Microextraction; D008297:Male; D055432:Metabolomics / Q000379:methods*; D016482:Urinalysis / Q000379:methods*
chemical_list: D000596:Amino Acids; D002264:Carboxylic Acids; D005466:Fluorocarbons; D005561:Formates; D007202:Indicators and Reagents; C570844:heptafluorobutyl chloroformate
keywords: GC–MS; Heptafluorobutyl chloroformate derivatization; Liquid-liquid microextraction; Metabolite profiling; Metabolomics; Urine