title: Proteomic Analysis of the Protein Expression Profile in the Mature Nigella sativa (Black Seed).
abstract: Nigella sativa (N. sativa) seed has been used as an important nutritional flavoring agent and in traditional medicine for treating many illnesses since ancient times. Understanding the proteomic component of the seed may lead to enhance the understanding of its structural and biological functional complexity. In this study, we have analyzed its proteome profile based on gel-based proteome mapping technique that includes one-dimensional gel electrophoresis followed by liquid chromatography and tandem mass spectrometry strategy. We have not come across any such studies that have been performed in N. sativa seeds up to date. A total of 277 proteins were identified, and their functional, metabolic, and location-wise annotations were carried out using the UniProt database. The majority of proteins identified in the proteome dataset based on their function were those involved in enzyme catalytic activity, nucleotide binding, and protein binding while the major cellular processes included regulation of biological process followed by regulation of secondary biological process, cell organization and biogenesis, protein metabolism, and transport. The identified proteome was localized mainly to the nucleus then to the cytoplasm, plasma membrane, mitochondria, plastid, and others. A majority of the proteins were involved in biochemical pathways involving carbohydrate metabolism, amino acid and shikimate pathway, lipid metabolism, nucleotide, cell organization and biogenesis, transport, and defense processes. The identified proteins in the dataset help to improve our understanding of the pathways involved in N. sativa seed metabolism and its biochemical features and detail out useful information that may help to utilize these proteins. This study could thus pave a way for future further high-throughput studies using a more targeted proteomic approach.
authors.affiliation: The National Center For Genomic Technology (NCGT), Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), P.O. Box 6086, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Ibrahim O
authors.initials: IO
authors.lastname: Alanazi
authors.affiliation: Obesity Research Center, College of Medicine, King Saud University, P.O. Box 2925 (98), Riyadh, 11461, Kingdom of Saudi Arabia.
authors.forename: Hicham
authors.initials: H
authors.lastname: Benabdelkamel
authors.affiliation: Obesity Research Center, College of Medicine, King Saud University, P.O. Box 2925 (98), Riyadh, 11461, Kingdom of Saudi Arabia.|Department of Medicine, College of Medicine, King Saud University, P.O. Box 2925 (38), Riyadh, 11461, Kingdom of Saudi Arabia.
authors.forename: Assim A
authors.initials: AA
authors.lastname: Alfadda
authors.affiliation: National Center for biotechnology, Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), P.O. Box 6086, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Sami A
authors.initials: SA
authors.lastname: AlYahya
authors.affiliation: The National Center For Genomic Technology (NCGT), Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), P.O. Box 6086, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Waleed M
authors.initials: WM
authors.lastname: Alghamdi
authors.affiliation: The National Center For Genomic Technology (NCGT), Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), P.O. Box 6086, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Hasan A
authors.initials: HA
authors.lastname: Aljohi
authors.affiliation: National Center for biotechnology, Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), P.O. Box 6086, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Abdulaziz
authors.initials: A
authors.lastname: Almalik
authors.affiliation: Obesity Research Center, College of Medicine, King Saud University, P.O. Box 2925 (98), Riyadh, 11461, Kingdom of Saudi Arabia. afsmasood@ksu.edu.sa.
authors.forename: Afshan
authors.initials: A
authors.lastname: Masood
mesh_terms: D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant; D031881:Nigella sativa / Q000235:genetics*; D010940:Plant Proteins / Q000096:biosynthesis*; D020543:Proteome / Q000235:genetics*; D040901:Proteomics; D053719:Tandem Mass Spectrometry
chemical_list: D010940:Plant Proteins; D020543:Proteome
keywords: 1D SDS PAGE; Biochemical pathways and LTQ Orbitrap XL; Nigella sativa (black seed); Seed proteome profiling