title: Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy.
abstract: Despite the progress made in selective plane illumination microscopy, high-resolution 3D live imaging of multicellular specimens remains challenging. Tiling light-sheet selective plane illumination microscopy (TLS-SPIM) with real-time light-sheet optimization was developed to respond to the challenge. It improves the 3D imaging ability of SPIM in resolving complex structures and optimizes SPIM live imaging performance by using a real-time adjustable tiling light sheet and creating a flexible compromise between spatial and temporal resolution. We demonstrate the 3D live imaging ability of TLS-SPIM by imaging cellular and subcellular behaviours in live C. elegans and zebrafish embryos, and show how TLS-SPIM can facilitate cell biology research in multicellular specimens by studying left-right symmetry breaking behaviour of C. elegans embryos.
authors.affiliation: Department of Chemistry, Stony Brook University, Stony Brook, New York 11794, USA.
authors.forename: Qinyi
authors.initials: Q
authors.lastname: Fu
authors.affiliation: Department of Biochemistry &Cell Biology, Stony Brook University, Stony Brook, New York 11794, USA.
authors.forename: Benjamin L
authors.initials: BL
authors.lastname: Martin
authors.affiliation: Department of Biochemistry &Cell Biology, Stony Brook University, Stony Brook, New York 11794, USA.
authors.forename: David Q
authors.identifier: http://orcid.org/0000-0002-1570-5025
authors.initials: DQ
authors.lastname: Matus
authors.affiliation: Department of Chemistry, Stony Brook University, Stony Brook, New York 11794, USA.|Department of Biochemistry &Cell Biology, Stony Brook University, Stony Brook, New York 11794, USA.
authors.forename: Liang
authors.initials: L
authors.lastname: Gao
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans; D004625:Embryo, Nonmammalian / Q000033:anatomy & histology*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D015027:Zebrafish