title: QTLs for Woolly Poplar Aphid (Phloeomyzus passerinii L.) Resistance Detected in an Inter-Specific Populus deltoides x P. nigra Mapping Population.
abstract: The genus Populus represents one of the most economically important groups of forest trees. It is composed by approximately 30 species used for wood and non-wood products, phytoremediation and biomass. Poplar is subjected to several biological and environmental threats although, compared to annual crops, we know far less about the genetic bases of biotic stress resistance. Woolly poplar aphid (Phloeomyzus passerinii) is considered a main pest of cultivated poplars in European and American countries. In this work we present two high density linkage maps in poplar obtained by a genotyping by sequencing (GBS) approach and the identification of QTLs involved in Ph. passerinii resistance. A total of 5,667 polymorphic markers (5,606 SNPs and 61 SSRs) identified on expressed sequences have been used to genotype 131 plants of an F1 population P ×canadensis obtained by an interspecific mate between Populus deltoides (resistant to woolly poplar aphid) and Populus nigra (susceptible to woolly poplar aphid). The two linkage maps, obtained following the two-way pseudo-testcross mapping strategy, have been used to investigate the genetic bases of woolly poplar aphid resistance. One major QTL and two QTLs with minor effects (mapped on LGV, LGXVI and LG XIX) explaining the 65.8% of the genetic variance observed in the progeny in response to Ph. passerinii attack were found. The high density coverage of functional markers allowed the identification of three genes belonging to disease resistance pathway as putative candidates for P. deltoides resistance to woolly poplar aphid. This work is the first report on genetic of woolly poplar aphid genetic resistance and the resistant loci associated markers identified represent a valuable tool in resistance poplar breeding programs.
authors.affiliation: Council for Agricultural Research and Economics (CREA)-Research Unit for Intensive Wood Production, Casale Monferrato (AL), Italy.
authors.forename: Giorgia
authors.initials: G
authors.lastname: Carletti
authors.affiliation: Council for Agricultural Research and Economics (CREA)-Research Unit for Intensive Wood Production, Casale Monferrato (AL), Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Carra
authors.affiliation: Council for Agricultural Research and Economics (CREA)-Research Unit for Intensive Wood Production, Casale Monferrato (AL), Italy.
authors.forename: Gianni
authors.initials: G
authors.lastname: Allegro
authors.affiliation: Council for Agricultural Research and Economics (CREA)-Research Unit for Intensive Wood Production, Casale Monferrato (AL), Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Vietto
authors.affiliation: Council for Agricultural Research and Economics (CREA)-Genomics Research Centre, Fiorenzuola d'Arda (PC), Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Desiderio
authors.affiliation: Council for Agricultural Research and Economics (CREA)-Genomics Research Centre, Fiorenzuola d'Arda (PC), Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Bagnaresi
authors.affiliation: Council for Agricultural Research and Economics (CREA)-Genomics Research Centre, Fiorenzuola d'Arda (PC), Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Gianinetti
authors.affiliation: Council for Agricultural Research and Economics (CREA)-Genomics Research Centre, Fiorenzuola d'Arda (PC), Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Cattivelli
authors.affiliation: Council for Agricultural Research and Economics (CREA)-Genomics Research Centre, Fiorenzuola d'Arda (PC), Italy.|Council for Agricultural Research and Economics (CREA)-Rice Research Unit, Vercelli, Italy.
authors.forename: Giampiero
authors.initials: G
authors.lastname: Valè
authors.affiliation: Council for Agricultural Research and Economics (CREA)-Research Unit for Intensive Wood Production, Casale Monferrato (AL), Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Nervo
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001042:Aphids / Q000502:physiology*; D001483:Base Sequence; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D060467:Disease Resistance / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers; D058977:Molecular Sequence Annotation; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology / Q000469:parasitology; D032107:Populus / Q000235:genetics* / Q000276:immunology / Q000469:parasitology*; D040641:Quantitative Trait Loci / Q000235:genetics*; D013045:Species Specificity
chemical_list: D005819:Genetic Markers