title: Model Construction and Analysis of Respiration in Halobacterium salinarum.
abstract: The archaeon Halobacterium salinarum can produce energy using three different processes, namely photosynthesis, oxidative phosphorylation and fermentation of arginine, and is thus a model organism in bioenergetics. Compared to its bacteriorhodopsin-driven photosynthesis, less attention has been devoted to modeling its respiratory pathway. We created a system of ordinary differential equations that models its oxidative phosphorylation. The model consists of the electron transport chain, the ATP synthase, the potassium uniport and the sodium-proton antiport. By fitting the model parameters to experimental data, we show that the model can explain data on proton motive force generation, ATP production, and the charge balancing of ions between the sodium-proton antiporter and the potassium uniport. We performed sensitivity analysis of the model parameters to determine how the model will respond to perturbations in parameter values. The model and the parameters we derived provide a resource that can be used for analytical studies of the bioenergetics of H. salinarum.
authors.affiliation: Institute of Mathematics, University of the Philippines, Diliman, Quezon City, Philippines.
authors.forename: Cherryl O
authors.initials: CO
authors.lastname: Talaue
authors.affiliation: Institute of Mathematics, University of the Philippines, Diliman, Quezon City, Philippines.|Max Planck Institute of Biochemistry, Department of Membrane Biochemistry, Martinsried, Germany.
authors.forename: Ricardo C H
authors.initials: RC
authors.lastname: del Rosario
authors.affiliation: Max Planck Institute of Biochemistry, Department of Membrane Biochemistry, Martinsried, Germany.
authors.forename: Friedhelm
authors.initials: F
authors.lastname: Pfeiffer
authors.affiliation: Institute of Mathematics, University of the Philippines, Diliman, Quezon City, Philippines.|Max Planck Institute of Biochemistry, Department of Membrane Biochemistry, Martinsried, Germany.
authors.forename: Eduardo R
authors.initials: ER
authors.lastname: Mendoza
authors.affiliation: Max Planck Institute of Biochemistry, Department of Membrane Biochemistry, Martinsried, Germany.
authors.forename: Dieter
authors.initials: D
authors.lastname: Oesterhelt
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D019843:Archaeal Proteins / Q000378:metabolism; D004579:Electron Transport; D004734:Energy Metabolism; D019617:Halobacterium salinarum / Q000378:metabolism*; D008954:Models, Biological; D010085:Oxidative Phosphorylation; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption; D011188:Potassium / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism
chemical_list: D019843:Archaeal Proteins; D017923:Sodium-Hydrogen Exchangers; D000255:Adenosine Triphosphate; D011188:Potassium; D010100:Oxygen