title: Mixture toxicity of water contaminants-effect analysis using the zebrafish embryo assay (Danio rerio).
abstract: Three water contaminants were selected to be tested in the zebrafish embryo toxicity test (DarT) in order to investigate the sensitivity of the zebrafish embryo toxicity test with respect to mixture effect detection. The concentration-response curves for the observed effects lethality and hypo-pigmentation were calculated after an exposure of the embryos for 96 h with a fungicide (carbendazim), a plasticizer or propellent precursor (2,4-DNT: 2,4- dinitrotoluene) and an aromatic compound (AαC: 2-amino-9H-pyrido[2,3-b]indol), respectively. Follow-up mixture tests were based on the calculated LC50 or EC50 of the single compounds and combined effects were predicted according to the mixture concepts of concentration addition (CA) and independent action (IA). The order of toxicity for the single substances was carbendazim (LC50 = 1.25 μM) < AαC (LC50 = 8.16 μM) < 2,4-DNT (LC50 = 177.05 μM). For AαC and 2,4 DNT hypo-pigmentation was observed in addition (AαC EC50 = 1.81 μM; 2,4-DNT EC50 = 8.81 μM). Two binary and one ternary mixture were studied on lethality and one on hypo-pigmentation: 2,4-DNT/AαC (LC50 = 119.21 μM, EC50 = 5.37 μM), carbendazim/AαC (LC50 = 4.49 μM) and AαC/Carbendazim/2,4 DNT (LC50 = 108.62 μM). Results showed that the effects were in agreement with the CA model when substances were tested in mixtures. Therefore, in a reasonable worst case scenario substance combination effects in fish embryos were at maximum only prone to overestimation when using CA as the mixture concept.
authors.affiliation: UFZ- Helmholtz Centre for Environmental Research, Department Bioanalytical Ecotoxicology, Permoserstr. 15, 04318 Leipzig, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Schmidt
authors.affiliation: UFZ- Helmholtz Centre for Environmental Research, Department Bioanalytical Ecotoxicology, Permoserstr. 15, 04318 Leipzig, Germany. Electronic address: Wibke.Busch@ufz.de.
authors.forename: Wibke
authors.initials: W
authors.lastname: Busch
authors.affiliation: UFZ- Helmholtz Centre for Environmental Research, Department Bioanalytical Ecotoxicology, Permoserstr. 15, 04318 Leipzig, Germany.
authors.forename: Rolf
authors.initials: R
authors.lastname: Altenburger
authors.affiliation: UFZ- Helmholtz Centre for Environmental Research, Department Bioanalytical Ecotoxicology, Permoserstr. 15, 04318 Leipzig, Germany.
authors.forename: Eberhard
authors.initials: E
authors.lastname: Küster
mesh_terms: D000818:Animals; D001562:Benzimidazoles / Q000633:toxicity*; D001681:Biological Assay; D002219:Carbamates / Q000633:toxicity*; D002243:Carbolines / Q000633:toxicity*; D004136:Dinitrobenzenes / Q000633:toxicity*; D004347:Drug Interactions; D004625:Embryo, Nonmammalian / Q000187:drug effects* / Q000378:metabolism; D005659:Fungicides, Industrial / Q000633:toxicity; D010860:Pigments, Biological / Q000378:metabolism; D010968:Plasticizers / Q000633:toxicity; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015027:Zebrafish
chemical_list: D001562:Benzimidazoles; D002219:Carbamates; D002243:Carbolines; D004136:Dinitrobenzenes; D005659:Fungicides, Industrial; D010860:Pigments, Biological; D010968:Plasticizers; D014874:Water Pollutants, Chemical; C016403:2,4-dinitrotoluene; C006698:carbendazim; C017822:2-amino-9H-pyrido(2,3-b)indole
keywords: Baseline toxicity; DNA damage; FET; Micro pollutant