title: Usefulness of the (GTG)4-PCR for typing of monophasic Salmonella enterica isolates with antigenic shame l,4,[5],12:i:-.
abstract: BACKGROUND
  Monophasic Salmonella enterica strains presenting the antigenic shame 1,4,[5],12:i:- are becoming more prevalent. Accurate identification of such strains is hard with routine using biochemical and serological tests. Such strains can be identified with molecular tests. In this study we have tested the usefulness of(GTG)4-PCR for the diagnostic of such monophasic strains. This usefulness of this method was previously confirmed for genoserotyping of S. Enterica, Typhimurium, Infantis, Virchow, Hadar, Newport and Anatum.
  
  
  METHODS
  76 strains with antigenic shame l,4,[5],12:i:-, isolated in Poland in years 2007-12 were tested. Additionally (GTG)4-PCR patterns were obtained for reference strains of serotypes S. Lagos, S. Agama, S. Farsta, S. Tsevie, S. Glocester and S. Tumodi. (GTG)4-PCR was performed with DreamTaq DNA polymerase. Obtained patterns were analysed with BioNumerics software.
  
  
  RESULTS
  No pattern specific for monophasic pattern was identified. Additionally it was also impossible to differentiate patterns obtained for S. Typhimurium, S. Farsta, S. Tsevie and S. Glocester. Only reference strains of serotypes S. Tumodi, Farsta and Agama has the distinguishable patterns of (GTG)4-PCR.
  
  
  CONCLUSIONS
  Analysed (GTG)4-PCR method do not show the ability to distinguish S. enterica serotypes from group 04, H:i, including monophasic strains with the antigenic shame 1,4,[5],12:i:-.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Wołkowicz
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Januszkiewicz
authors.forename: Anna
authors.initials: A
authors.lastname: Chróst
authors.forename: Natalia
authors.initials: N
authors.lastname: Wolaniuk
authors.forename: Anna B
authors.initials: AB
authors.lastname: Kubiak
authors.forename: Marta
authors.initials: M
authors.lastname: Majchrzak
authors.forename: Jolanta
authors.initials: J
authors.lastname: Szych
authors.forename: Paweł
authors.initials: P
authors.lastname: Parniewski
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D011044:Poland; D016133:Polymerase Chain Reaction / Q000379:methods*; D019779:Salmonella enterica / Q000145:classification* / Q000302:isolation & purification