title: Magnetic field contributes to the cellular uptake for effective therapy with magnetofection using plasmid DNA encoding against Mcam in B16F10 melanoma in vivo.
abstract: OBJECTIVE
  We explored the distribution and cellular uptake of intratumorally injected SPIONs-PAA-PEI-pDNA (magnetofection complexes), and antitumor effectiveness of magnetofection with plasmid DNA encoding short hairpin RNA (shRNA) against Mcam (pDNA(anti-MCAM)).
  
  
  METHODS
  Analyses were made based on the histology, ultrastructure and quantitative measurements of magnetofection complexes, and quantification of the antitumor effectiveness in B16F10 melanoma in vivo.
  
  
  RESULTS
  Injected magnetofection complexes were distributed around the injection site. Exposure of tumors to external magnetic field contributed to the uptake of magnetofection complexes from extracellular matrix into melanoma cells. Three consecutive magnetofections of tumors with pDNA(anti-MCAM) resulted in significant reduction of tumor volume.
  
  
  CONCLUSIONS
  Magnetofection is effective for gene delivery to melanoma tumors, but requires a magnetic field for cellular uptake and antitumor effect.
authors.affiliation: Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska 2, SI-1000 Ljubljana, Slovenia.
authors.forename: Lara
authors.initials: L
authors.lastname: Prosen
authors.affiliation: Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, SI-1000 Ljubljana, Slovenia.
authors.forename: Samo
authors.initials: S
authors.lastname: Hudoklin
authors.affiliation: Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska 2, SI-1000 Ljubljana, Slovenia.|Faculty of Health Sciences, University of Primorska, Polje 42, SI-6310 Izola, Slovenia.
authors.forename: Maja
authors.initials: M
authors.lastname: Cemazar
authors.affiliation: Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska 2, SI-1000 Ljubljana, Slovenia.
authors.forename: Monika
authors.initials: M
authors.lastname: Stimac
authors.affiliation: Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska 2, SI-1000 Ljubljana, Slovenia.
authors.forename: Ursa
authors.initials: U
authors.lastname: Lampreht Tratar
authors.affiliation: Department of Pathology, Institute of Oncology Ljubljana, Zaloska 2, SI-1000 Ljubljana, Slovenia.
authors.forename: Maja
authors.initials: M
authors.lastname: Ota
authors.affiliation: Department of Environmental Sciences, Jozef Stefan Institute, Jamova 39, SI-1000 Ljubljana, Slovenia.
authors.forename: Janez
authors.initials: J
authors.lastname: Scancar
authors.affiliation: Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, SI-1000 Ljubljana, Slovenia.
authors.forename: Rok
authors.initials: R
authors.lastname: Romih
authors.affiliation: Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska 2, SI-1000 Ljubljana, Slovenia.
authors.forename: Gregor
authors.initials: G
authors.lastname: Sersa
mesh_terms: D000818:Animals; D051929:CD146 Antigen / Q000235:genetics; D045744:Cell Line, Tumor; D004247:DNA / Q000235:genetics / Q000493:pharmacokinetics / Q000627:therapeutic use*; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D060526:Magnetic Fields; D058185:Magnetite Nanoparticles / Q000737:chemistry; D008545:Melanoma / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D008810:Mice, Inbred C57BL; D010957:Plasmids / Q000235:genetics / Q000493:pharmacokinetics / Q000627:therapeutic use*; D011094:Polyethyleneimine / Q000737:chemistry; D034741:RNA, Small Interfering / Q000235:genetics*
chemical_list: D051929:CD146 Antigen; D058185:Magnetite Nanoparticles; C497903:Mcam protein, mouse; D034741:RNA, Small Interfering; D011094:Polyethyleneimine; D004247:DNA
keywords: MCAM; SPIONs; cellular uptake; intratumoral injection; magnetofection; murine melanoma tumor; therapeutic plasmid DNA