title: Structural elucidation of a pectin from flowers of Lonicera japonica and its antipancreatic cancer activity.
abstract: To investigate polysaccharide structure from Lonicera japonica, and study its effects on behavior of pancreatic cells, a homogenous polysaccharide, LJ-02-1, was extracted and purified from flowers of L. japonica by DEAE-cellulose and Sephacryl S-200HR column. The molecular weight was estimated to be 54kDa. Monosaccharide composition was determined to be rhamnose, galacturonic acid, galactose and arabinose in the molar ratio of 10.77:7.88:15.45:65.89 by analyzing the PMP derivatives of the monosaccharides from 2M trifluoracetic acid hydrolysis via HPLC. Based on methylation analysis, partial acid hydrolysis, and NMR spectra, the polysaccharide was elucidated to be a rhamnogalacturonan backbone and substituted partly at C-4 of rhamnose. The branches were determined to be T- and 1,4,6-linked β-d-Galp, T- and 1,5-linked α-l-Araf. The polysaccharide might inhibit BxPC-3 and PANC-1 pancreatic cancer cells growth at the concentration of 1mg/mL with inhibitory ratio of 66.7% and 52.1%, respectively.
authors.affiliation: School of Pharmacy, Zunyi Medical University, 201 Dalian Road, Zunyi 563003, PR China; Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Lin
authors.affiliation: Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Wang
authors.affiliation: Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China.
authors.forename: Zhenyun
authors.initials: Z
authors.lastname: Du
authors.affiliation: Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China.
authors.forename: Wucheng
authors.initials: W
authors.lastname: Wang
authors.affiliation: Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China.
authors.forename: Qifei
authors.initials: Q
authors.lastname: Cong
authors.affiliation: Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China.
authors.forename: Changping
authors.initials: C
authors.lastname: Zheng
authors.affiliation: School of Pharmacy, Zunyi Medical University, 201 Dalian Road, Zunyi 563003, PR China; Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China.
authors.forename: Can
authors.initials: C
authors.lastname: Jin
authors.affiliation: School of Pharmacy, Zunyi Medical University, 201 Dalian Road, Zunyi 563003, PR China; Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China. Electronic address: dingkan@simm.ac.cn.
authors.forename: Kan
authors.initials: K
authors.lastname: Ding
authors.affiliation: Department of General Surgery Shanghai Changzheng Hospital, No. 415 Fengyang Road, Shanghai 200003, PR China. Electronic address: 13801938229@163.com.
authors.forename: Chenghao
authors.initials: C
authors.lastname: Shao
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002240:Carbohydrate Sequence; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects* / Q000473:pathology; D035264:Flowers / Q000737:chemistry*; D006801:Humans; D006868:Hydrolysis; D031239:Lonicera / Q000737:chemistry*; D008970:Molecular Weight; D010179:Pancreas / Q000187:drug effects / Q000473:pathology; D010368:Pectins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D012210:Rhamnose / Q000737:chemistry / Q000302:isolation & purification; D014269:Trifluoroacetic Acid / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C473998:rhamnogalacturonan J1; D010368:Pectins; D014269:Trifluoroacetic Acid; D012210:Rhamnose
keywords: Antipancreatic cancer cells growth; Lonicera japonica; Polysaccharide