title: Protective effects of acerola juice on genotoxicity induced by iron in vivo.
abstract: Metal ions such as iron can induce DNA damage by inducing reactive oxygen species (ROS) and oxidative stress. Vitamin C is one of the most widely consumed antioxidants worldwide, present in many fruits and vegetables, especially inMalpighia glabra L., popularly known as acerola, native to Brazil. Acerola is considered a functional fruit due to its high antioxidant properties and phenolic contents, and therefore is consumed to prevent diseases or as adjuvant in treatment strategies. Here, the influence of ripe and unripe acerola juices on iron genotoxicity was analyzed in vivo using the comet assay and micronucleus test. The comet assay results showed that acerola juice exerted no genotoxic or antigenotoxic activity. Neither ripe nor unripe acerola juices were mutagenic to animals treated with juices, in micronucleus test. However, when compared to iron group, the pre-treatment with acerola juices exerted antimutagenic activity, decreasing significantly micronucleus mean values in bone marrow. Stage of ripeness did not influence the interaction of acerola compounds with DNA, and both ripe and unripe acerola juices exerted protective effect over DNA damage generated by iron.
authors.affiliation: Centro de Ciências da Saúde, Universidade da Região da Campanha, Bagé, RS, Brazil.
authors.forename: Roberta Nunes
authors.initials: RN
authors.lastname: Horta
authors.affiliation: Laboratório de Genética Toxicológica, Universidade Luterana do Brasil, Canoas, RS, Brazil.
authors.forename: Vivian Francilia Silva
authors.initials: VF
authors.lastname: Kahl
authors.affiliation: Laboratório de Genética Toxicológica, Universidade Luterana do Brasil, Canoas, RS, Brazil.
authors.forename: Merielen da Silva
authors.initials: Mda S
authors.lastname: Sarmento
authors.affiliation: Centro de Ciências da Saúde, Universidade da Região da Campanha, Bagé, RS, Brazil.
authors.forename: Marisa Fernanda Silva
authors.initials: MF
authors.lastname: Nunes
authors.affiliation: Laboratório de Genética Toxicológica, Universidade Luterana do Brasil, Canoas, RS, Brazil.
authors.forename: Carem Rejane Maglione
authors.initials: CR
authors.lastname: Porto
authors.affiliation: Laboratório de Biologia Celular e Molecular, Unidade de Ciências de Saúde, Universidade do Estado de Santa Catarina, Criciúma, SC, Brazil.
authors.forename: Vanessa Moraes de
authors.initials: VM
authors.lastname: Andrade
authors.affiliation: Laboratório do Farmacognosia e Fitoquímica, Universidade Luterana do Brasil, Canoas, RS, Brazil.
authors.forename: Alexandre de Barros Falcão
authors.initials: Ade B
authors.lastname: Ferraz
authors.affiliation: Laboratório de Genética Toxicológica, Universidade Luterana do Brasil, Canoas, RS, Brazil.
authors.forename: Juliana Da
authors.initials: JD
authors.lastname: Silva