title: Development and validation of a sensitive LC-MS/MS assay for quantification of bruceine D in rat plasma.
abstract: A sensitive LC-MS/MS method for the determination of bruceine D in rat plasma was developed. The analyte and IS were separated on a Luna C18column (2.1 × 50 mm, 1.7 μm) using a mobile phase of acetonitrile and 0.1% formic acid in water (40:60, v/v) at a flow rate of 0.25 mL/min. The selected reaction monitoring mode was chosen to monitor the precursor-to-product ion transitions of m/z 409.2 → 373.2 for bruceine D and m/z 469.2 → 229.3 for IS using a negative ESI mode. The method was validated over a concentration range of 0.5-2000 ng/mL for bruceine D. Total chromatography time for each run was 3.5 min. The method was successfully applied to a pharmacokinetic study of bruceine D in rats. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Pediatrics, the First Hospital of Jilin University, Jilin University, Changchun, 130021, China.
authors.forename: Yining
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Northern Jiangsu People's Hospital, Yangzhou, 225001, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhen
authors.affiliation: Department of Pediatrics, the First Hospital of Jilin University, Jilin University, Changchun, 130021, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, the Second Hospital of Jilin University, Changchun, 130041, China. syliu_tgnm@hotmail.com.
authors.forename: Shouyue
authors.initials: S
authors.lastname: Liu
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000032:analysis / Q000097:blood*; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis / Q000097:blood*; D032269:Brucea / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D008297:Male; D036702:Quassins / Q000032:analysis / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000962:Antimalarials; D000972:Antineoplastic Agents, Phytogenic; D036702:Quassins; C030412:bruceine D
keywords: Lc-Ms/Ms; bruceine D; pharmacokinetic study