title: Diagnosis of latent tuberculosis by ELISPOT assay and tuberculin skin test.
abstract: OBJECTIVE
  To determine the prevalence of latent tuberculosis infection (LTBI) in college students.
  
  
  METHODS
  Four hundred and twenty newly admitted college students were enrolled. The Enzyme-Linked ImmunoSpot assay (ELISPOT) was used. Overall, 171 students with ELISPOT assay+/TST+ were monitored for three years to detect active TB disease.
  
  
  RESULTS
  The overall positive rate of ELISPOT assay was 40.7% among TST+ students. The ELISPOT positive rates were 36.9%, 45.4%, and 64.3% in groups of TST induration of 10-14mm, 15-20mm, and ≥20mm, respectively, with a significant difference (χ(2)=10.136, P<0.01) but no significant difference between BCG scar and no scar (41.2% vs. 38.8%; P>0.05). None of the 171 untreated students contracted active TB within the three-year monitoring period.
  
  
  CONCLUSIONS
  The LTBI rate might be overestimated by TST compared with interferon-γ release assays. On the basis of a close monitoring, few students developed active TB despite a positive result to the TST and ELISPOT assay.
authors.affiliation: Beijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, 101149 Beijing, China.
authors.forename: F
authors.initials: F
authors.lastname: Du
authors.affiliation: Changping Tuberculosis Prevent and Control Institute of Beijing, 102206 Beijing, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Changping Tuberculosis Prevent and Control Institute of Beijing, 102206 Beijing, China.
authors.forename: T
authors.initials: T
authors.lastname: Gao
authors.affiliation: Beijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, 101149 Beijing, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Beijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, 101149 Beijing, China.
authors.forename: H
authors.initials: H
authors.lastname: Jia
authors.affiliation: Beijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, 101149 Beijing, China.
authors.forename: A
authors.initials: A
authors.lastname: Xing
authors.affiliation: Beijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, 101149 Beijing, China.
authors.forename: B
authors.initials: B
authors.lastname: Du
authors.affiliation: Beijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, 101149 Beijing, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Beijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, 101149 Beijing, China.
authors.forename: T
authors.initials: T
authors.lastname: Cao
authors.affiliation: Beijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, 101149 Beijing, China. Electronic address: zzd417@163.com.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D001500:BCG Vaccine; D002681:China / Q000453:epidemiology; D058501:Enzyme-Linked Immunospot Assay; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D059425:Interferon-gamma Release Tests; D055985:Latent Tuberculosis / Q000175:diagnosis*; D008297:Male; D015995:Prevalence; D011446:Prospective Studies; D011993:Recombinant Fusion Proteins / Q000276:immunology; D013334:Students / Q000706:statistics & numerical data; D014374:Tuberculin Test; D014495:Universities; D000073887:Vaccination Coverage; D055815:Young Adult
chemical_list: D001500:BCG Vaccine; C505464:ESAT-6-CFP10 fusion protein; D011993:Recombinant Fusion Proteins
keywords: Interferon-γ; Interféron-γ; Intradermoréaction à la tuberculine; Latent tuberculosis; Tuberculin skin test; Tuberculose latente