title: Functional specialization of one copy of glutamine phosphoribosyl pyrophosphate amidotransferase in ureide production from symbiotically fixed nitrogen in Phaseolus vulgaris.
abstract: Purines are essential molecules formed in a highly regulated pathway in all organisms. In tropical legumes, the nitrogen fixed in the nodules is used to generate ureides through the oxidation of de novo synthesized purines. Glutamine phosphoribosyl pyrophosphate amidotransferase (PRAT) catalyses the first committed step of de novo purine synthesis. In Phaseolus vulgaris there are three genes coding for PRAT. The three full-length sequences, which are intron-less genes, were cloned, and their expression levels were determined under conditions that affect the synthesis of purines. One of the three genes, PvPRAT3, is highly expressed in nodules and protein amount and enzymatic activity in these tissues correlate with nitrogen fixation activity. Inhibition of PvPRAT3 gene expression by RNAi-silencing and subsequent metabolomic analysis of the transformed roots shows that PvPRAT3 is essential for the synthesis of ureides in P. vulgaris nodules.
authors.affiliation: Departamento de Botánica, Ecología y Fisiología Vegetal, Grupo de Fisiología Molecular y Biotecnología de Plantas. Campus de Excelencia Internacional Agroalimentario, CEIA3. Campus de Rabanales, Edif. Severo Ochoa, Universidad de Córdoba, 14071, Córdoba, Spain.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Coleto
authors.affiliation: Departamento de Botánica, Ecología y Fisiología Vegetal, Grupo de Fisiología Molecular y Biotecnología de Plantas. Campus de Excelencia Internacional Agroalimentario, CEIA3. Campus de Rabanales, Edif. Severo Ochoa, Universidad de Córdoba, 14071, Córdoba, Spain.
authors.forename: Almudena T
authors.initials: AT
authors.lastname: Trenas
authors.affiliation: Departamento de Botánica, Ecología y Fisiología Vegetal, Grupo de Fisiología Molecular y Biotecnología de Plantas. Campus de Excelencia Internacional Agroalimentario, CEIA3. Campus de Rabanales, Edif. Severo Ochoa, Universidad de Córdoba, 14071, Córdoba, Spain.
authors.forename: Alexander
authors.initials: A
authors.lastname: Erban
authors.affiliation: Departamento de Botánica, Ecología y Fisiología Vegetal, Grupo de Fisiología Molecular y Biotecnología de Plantas. Campus de Excelencia Internacional Agroalimentario, CEIA3. Campus de Rabanales, Edif. Severo Ochoa, Universidad de Córdoba, 14071, Córdoba, Spain.
authors.forename: Joachim
authors.initials: J
authors.lastname: Kopka
authors.affiliation: Departamento de Botánica, Ecología y Fisiología Vegetal, Grupo de Fisiología Molecular y Biotecnología de Plantas. Campus de Excelencia Internacional Agroalimentario, CEIA3. Campus de Rabanales, Edif. Severo Ochoa, Universidad de Córdoba, 14071, Córdoba, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Pineda
authors.affiliation: Departamento de Botánica, Ecología y Fisiología Vegetal, Grupo de Fisiología Molecular y Biotecnología de Plantas. Campus de Excelencia Internacional Agroalimentario, CEIA3. Campus de Rabanales, Edif. Severo Ochoa, Universidad de Córdoba, 14071, Córdoba, Spain.
authors.forename: Josefa M
authors.initials: JM
authors.lastname: Alamillo
mesh_terms: D000582:Amidophosphoribosyltransferase / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D007527:Isoenzymes / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D009586:Nitrogen Fixation; D027805:Phaseolus / Q000201:enzymology* / Q000235:genetics; D053204:Root Nodules, Plant / Q000378:metabolism*; D017422:Sequence Analysis, DNA
chemical_list: D007527:Isoenzymes; D000582:Amidophosphoribosyltransferase; D009584:Nitrogen
keywords: common bean; nitrogen fixation; purines