title: Effect of granulocyte colony-stimulating factor administration on renal regeneration after experimentally induced acute kidney injury in dogs.
abstract: OBJECTIVE
  To evaluate the effects of granulocyte colony-stimulating factor (GCSF) administration in dogs with experimentally induced acute kidney injury.
  
  
  METHODS
  6 healthy dogs.
  
  
  METHODS
  After induction of kidney injury (day 0) with cisplatin (5 mg/kg, IV), the dogs were randomly assigned into 2 groups (n = 3 dogs/group). Then dogs immediately received GCSF (10 μg/kg) or 1 mL of saline (0.9% NaCl) solution (control group) SC; this treatment was repeated once daily for 4 additional days (days 1 through 4). A once-daily CBC (day 0 to 4), serum biochemical analysis (day 0 to 3), and urinalysis (day 0 to 3) were performed for each dog; samples were collected before administration of cisplatin (day 0) and before treatment with GCSF or saline solution (days 1 through 4). After sample collection and treatment on day 4, all dogs were euthanized; kidney tissue samples underwent histologic evaluation, immunohistochemical analyses, and cytokine profiling via reverse transcriptase PCR assay.
  
  
  RESULTS
  In the GCSF-treated group, the histologic evaluation and immunohistochemical analyses of kidney tissue revealed less fibrotic change and greater proliferation of renal tubular epithelial cells, compared with findings in the control group. The mRNA profiles of kidney tissue from the GCSF-treated group indicated lower expression of tumor necrosis factor-α and tumor growth factor-β, compared with findings in the control group; however, concentrations of factors related to renal regeneration were not greater in the GCSF-treated group.
  
  
  CONCLUSIONS
  Results indicated that GCSF treatment can impede renal fibrosis and increase proliferation of renal tubules after experimentally induced acute kidney injury in dogs.
authors.forename: Chae-Young
authors.initials: CY
authors.lastname: Lim
authors.forename: Jae-Ik
authors.initials: JI
authors.lastname: Han
authors.forename: Seung-Gon
authors.initials: SG
authors.lastname: Kim
authors.forename: Chang-Min
authors.initials: CM
authors.lastname: Lee
authors.forename: Hee-Myung
authors.initials: HM
authors.lastname: Park
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced / Q000188:drug therapy / Q000662:veterinary*; D000818:Animals; D002945:Cisplatin / Q000633:toxicity; D004283:Dog Diseases / Q000139:chemically induced* / Q000188:drug therapy; D004285:Dogs; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects; D012038:Regeneration / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha
chemical_list: D014409:Tumor Necrosis Factor-alpha; D016179:Granulocyte Colony-Stimulating Factor; D002945:Cisplatin