title: Hydrolysis treatments of fruit and vegetable waste for production of biofuel precursors.
abstract: This study investigated hydrolysis approaches for cultivation of the oleaginous red yeast Rhodotorula glutinis for biodiesel production, whilst utilising the residual solids (RS) for biogas production. Macerated fruit and vegetable waste (FVW) (24h-4°C-leachate served as the control, Pcon) was hydrolysed chemically (Chem), thermally (Therm) and using a combined thermo-chemical treatment (T-Chem). All cleared hydrolysates supported growth of R. glutinis, which was nitrogen-limited. T-Chem hydrolysates yielded highest biomass, total fatty acids (TotFA) and RS-derived biogas yields, biomass TotFA failed to meet standards for fuel density and higher heating values, met by the other treatments. Even though Pcon-derived yields were slightly lower, it is recommended for FVW treatment for local biogas and biodiesel production due to energy and environmental impact considerations.
authors.affiliation: College of Marine and Environmental Sciences, James Cook University, Townsville 4811, Queensland, Australia; Comparative Genomics Centre, James Cook University, Townsville 4811, Queensland, Australia; Centre for Bio-discovery and Molecular Development of Therapeutics, James Cook University, Townsville 4811, Queensland, Australia.
authors.forename: Ali
authors.initials: A
authors.lastname: Razaghi
authors.affiliation: College of Marine and Environmental Sciences, James Cook University, Townsville 4811, Queensland, Australia; Comparative Genomics Centre, James Cook University, Townsville 4811, Queensland, Australia.
authors.forename: O P
authors.initials: OP
authors.lastname: Karthikeyan
authors.affiliation: College of Marine and Environmental Sciences, James Cook University, Townsville 4811, Queensland, Australia.
authors.forename: H T Nguyen
authors.initials: HT
authors.lastname: Hao
authors.affiliation: College of Marine and Environmental Sciences, James Cook University, Townsville 4811, Queensland, Australia; Comparative Genomics Centre, James Cook University, Townsville 4811, Queensland, Australia; Centre for Bio-discovery and Molecular Development of Therapeutics, James Cook University, Townsville 4811, Queensland, Australia; Centre for Sustainable Tropical Fisheries and Aquaculture, James Cook University, Townsville 4811, Queensland, Australia. Electronic address: kirsten.heimann@jcu.edu.au.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Heimann
mesh_terms: D056804:Biofuels; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002241:Carbohydrates / Q000032:analysis; D004952:Esters / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005638:Fruit / Q000737:chemistry*; D006868:Hydrolysis; D008697:Methane / Q000378:metabolism; D012248:Rhodotorula / Q000254:growth & development / Q000378:metabolism; D014675:Vegetables / Q000737:chemistry*; D014866:Waste Products
chemical_list: D056804:Biofuels; D002241:Carbohydrates; D004952:Esters; D005227:Fatty Acids; D014866:Waste Products; D008697:Methane
keywords: Biodiesel; Fruit and vegetable waste; Hydrolysis; Methane; Rhodotorula glutinis