title: Transcriptomic response to water accommodated fraction of crude oil exposure in the gill of Japanese flounder, Paralichthys olivaceus.
abstract: Illumina-based RNA-seq was used to determine the short-term transcriptomic responses of Paralichthys olivaceus gill to an environmentally relevant level of water accommodated fraction (WAF) of crude oil. 213,979 transcripts and 128,482 unigenes were obtained. Differential expression analysis revealed that 1641 and 2142 genes were significantly up- and down-regulated. Enrichment analysis identified a set of GO terms and putative pathways involved in the response of P. olivaceus to WAF exposure. Analysis of the transcripts revealed the effective protective mechanisms of P. olivaceus to reduce the toxic effects of WAF. Moreover, WAF exposure induced the metabolism of energy substrates, and downstream pathway genes were modified to provide protection against toxic damage. Transcripts analysis demonstrated that the genes involved in circadian rhythm signaling were regulated in gills of P. olivaceus exposed to WAF. These results provide insights into the mechanisms of WAF-induced toxicity in fishes and into the WAF-sensitive biomarkers in P. olivaceus.
authors.affiliation: Marine Fishery Environment and Bioremediation Laboratory, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Marine Fishery Environment and Bioremediation Laboratory, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
authors.forename: Keming
authors.initials: K
authors.lastname: Qu
authors.affiliation: Marine Fishery Environment and Bioremediation Laboratory, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266200, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Xia
authors.affiliation: Marine Fishery Environment and Bioremediation Laboratory, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Sun
authors.affiliation: Marine Fishery Environment and Bioremediation Laboratory, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China. Electronic address: chenbj@ysfri.ac.cn.
authors.forename: Bijuan
authors.initials: B
authors.lastname: Chen
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D005432:Flounder / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D005880:Gills / Q000187:drug effects* / Q000378:metabolism; D008962:Models, Theoretical; D010578:Petroleum / Q000378:metabolism / Q000633:toxicity*; D059392:Petroleum Pollution / Q000009:adverse effects* / Q000032:analysis; D059467:Transcriptome / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D010578:Petroleum; D014874:Water Pollutants, Chemical
keywords: Gill; Japanese flounder Paralichthys olivaceus; Oil spill; RNA-seq; Transcriptome