title: Kinetics of cytokine profile in response to Mycobacterium bovis BCG and Streptococcus pyogenes activated cells.
abstract: The infection of epithelial cells is a necessary step for Mycobacterium bovis BCG dissemination, but the mechanism of mycobacterial epithelial interactions is not completely understood. Similarly, Streptococcus pyogenes is a strictly human pathogen that favorably colonizes the skin and the pharynx. Effective cytokine secretion is essential in order to fabricate a suitable inflammatory response against an infection. In this data article, the cytokine profile in BCG and S. pyogenes activated THP-1 cell line in media after the acute phase of infection by ELISA is described. The interleukin-8 level was increased in response to both BCG and S. pyogenes, but was quite prominent after 24 h and further increased upto 72 h post infection. On the other hand, an increase in IL-6 response to S. pyogenes was observed while there was no response to BCG even after 48 h of infection. A low level of TNF-α was detected upon BCG and S. pyogenes infection.
authors.affiliation: Dr. B.R. Ambedkar Centre for Biomedical Research (ACBR), University of Delhi, Delhi 110007, India.
authors.forename: Vivek
authors.initials: V
authors.lastname: Verma
authors.affiliation: Dr. B.R. Ambedkar Centre for Biomedical Research (ACBR), University of Delhi, Delhi 110007, India.
authors.forename: Parveen
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Department of Translational and Regenerative Medicine, Post Graduate Institute of Medical Education & Research (PGIMER), Chandigarh 160012, India.
authors.forename: Rakesh Singh
authors.initials: RS
authors.lastname: Dhanda
authors.affiliation: Dr. B.R. Ambedkar Centre for Biomedical Research (ACBR), University of Delhi, Delhi 110007, India.
authors.forename: Manisha
authors.initials: M
authors.lastname: Yadav
keywords: Cytokines; IL-6; IL-8; Mycobacterium bovis BCG; Streptococcus pyogenes; TNF-α