title: Microfluidic technologies for yeast replicative lifespan studies.
abstract: The budding yeast Saccharomyces cerevisiae has been used as a model organism for the study of aging for over 50 years. In this time, the canonical aging experiment-replicative lifespan analysis by manual microdissection-has remained essentially unchanged. Recently, microfluidic technologies have been developed that may be able to substitute for this time- and labor-intensive procedure. These technologies also allow cell physiology to be observed throughout the entire lifetime. Here, we review these devices, novel observations they have made possible, and some of the current system limitations.
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA, USA; Department of Genome Sciences, University of Washington, Seattle, WA, USA; Medical Scientist Training Program, University of Washington, Seattle, WA, USA.
authors.forename: Kenneth L
authors.initials: KL
authors.lastname: Chen
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA, USA.
authors.forename: Matthew M
authors.initials: MM
authors.lastname: Crane
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA, USA. Electronic address: kaeber@uw.edu.
authors.forename: Matt
authors.initials: M
authors.lastname: Kaeberlein
mesh_terms: D046210:Microfluidic Analytical Techniques / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*
keywords: Lifespan; Microdissection; Microfluidics; Microscopy; Replicative aging; Yeast