title: Alternative splicing directs two IL-20R2 isoforms and is responsible for the incomplete gene knockout via the exon I ablation.
abstract: Two heterodimeric receptors consisting of interleukin (IL)-20R2 are shared by three of the IL-20 family of cytokines, IL-19, IL-20 and IL-24. Along with IL-22, these cytokines are downstream effectors of IL-23 and have been implicated in keratinocyte functions and the pathogenesis of psoriasis. Surprisingly, whereas knocking out either the IL-23 or IL-22 gene abolished imiquimod-induced psoriatic phenotypes in mice, similar attempt for IL-20R2 had little effect. Here, we report that the apparent disparity may result from a new IL-20R2 isoform encoded by an alternatively spliced transcript which survived the previous attempt for IL-20R2 gene knockout via the exon I deletion.
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Life Sciences, Sichuan University, Chengdu, China.
authors.forename: H
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Life Sciences, Sichuan University, Chengdu, China.
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Life Sciences, Sichuan University, Chengdu, China.
authors.forename: R
authors.initials: R
authors.lastname: Yu
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Life Sciences, Sichuan University, Chengdu, China.
authors.forename: T
authors.initials: T
authors.lastname: Long
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Life Sciences, Sichuan University, Chengdu, China.
authors.forename: R
authors.initials: R
authors.lastname: Zhao
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Life Sciences, Sichuan University, Chengdu, China.
authors.forename: H
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Life Sciences, Sichuan University, Chengdu, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Clover Biopharmaceuticals, Chengdu, China.
authors.forename: J G
authors.initials: JG
authors.lastname: Liang
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Life Sciences, Sichuan University, Chengdu, China.|Clover Biopharmaceuticals, Chengdu, China.|Laboratory for Gene and Cell Therapy, Sichuan University, Chengdu, China.|GenHunter Corporation, Nashville, TN, USA.
authors.forename: P
authors.initials: P
authors.lastname: Liang
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D005091:Exons; D017353:Gene Deletion; D053759:Interleukin-23 / Q000235:genetics / Q000378:metabolism; D007378:Interleukins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D018123:Receptors, Interleukin / Q000235:genetics* / Q000378:metabolism; D000097043:Interleukin-22
chemical_list: D053759:Interleukin-23; D007378:Interleukins; D020033:Protein Isoforms; D018123:Receptors, Interleukin; D000097043:Interleukin-22; C492510:interleukin-20 receptor