title: Sialoglycoprotein Isolated from Eggs of Carassius auratus Ameliorates Osteoporosis: An Effect Associated with Regulation of the Wnt/β-Catenin Pathway in Rodents.
abstract: In the current study, ovariectomized (OVX) rats and the senescence-accelerated mouse strain P6 (SAMP6) were employed to establish models of postmenopausal osteoporosis and senile osteoporosis, respectively. The effects of treatment with sialoglycoprotein isolated from the eggs of Carassius auratus (Ca-SGP) on these two types of osteoporosis were investigated in vivo. Results showed that Ca-SGP significantly increased bone mineral density, ameliorated trabecular bone microstructure, and improved bone biomechanical properties in both OVX rats and SAMP6. The osteogenesis related Wnt/β-catenin pathway was targeted to study the underlying mechanism of Ca-SGP activity. In postmenopausal osteoporosis, Ca-SGP suppressed the activation of Wnt/β-catenin signal via down-regulating the expression of key genes including LRP5, β-catenin, and Runx2, suggesting that overactive osteogenesis was controlled by Ca-SGP. The bone formation was sharply weakened in senile osteoporosis, whereas Ca-SGP treatment promoted osteoblast activity by stimulating the Wnt/β-catenin signal. In conclusion, Ca-SGP ameliorated these two types of osteoporosis by normalizing bone anabolism.
authors.affiliation: College of Food Science and Engineering, Ocean University of China , Qingdao, Shandong Province 266003, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Ocean University of China , Qingdao, Shandong Province 266003, China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Ocean University of China , Qingdao, Shandong Province 266003, China.
authors.forename: Yanlei
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Food Science and Engineering, Ocean University of China , Qingdao, Shandong Province 266003, China.
authors.forename: Qiping
authors.initials: Q
authors.lastname: Zhan
authors.affiliation: College of Food Science and Engineering, Ocean University of China , Qingdao, Shandong Province 266003, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Yu
authors.affiliation: College of Food Science and Engineering, Ocean University of China , Qingdao, Shandong Province 266003, China.
authors.forename: Jingfeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Ocean University of China , Qingdao, Shandong Province 266003, China.
authors.forename: Changhu
authors.initials: C
authors.lastname: Xue
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism; D004531:Eggs / Q000032:analysis*; D005260:Female; D029941:Fish Proteins / Q000008:administration & dosage* / Q000302:isolation & purification; D005786:Gene Expression Regulation; D006054:Goldfish; D006801:Humans; D008297:Male; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism; D010024:Osteoporosis / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D015663:Osteoporosis, Postmenopausal / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D051381:Rats; D012795:Sialoglycoproteins / Q000008:administration & dosage* / Q000302:isolation & purification; D060449:Wnt Signaling Pathway / Q000187:drug effects*; D051176:beta Catenin / Q000378:metabolism
chemical_list: D029941:Fish Proteins; D012795:Sialoglycoproteins; D051176:beta Catenin
keywords: Carassius auratus eggs; OVX; SAMP6; Wnt/β-catenin; sialoglycoprotein