title: Polymorphisms in human telomerase reverse transcriptase (hTERT) gene and susceptibility to gastric cancer in a Turkish population: Hospital-based case-control study.
abstract: Erosion of telomeres, tandem nucleotide repeats (TTAGGG)n that cap the end of eukaryotic chromosomes, has been related with carcinogenesis. The human telomerase reverse transcriptase (hTERT) gene is encoded the rate-limiting catalytic subunit of the telomerase complexes, which is essential for the protection of telomeric DNA length and chromosomal stability. The purpose of this study was to examine the effect of four functional single nucleotide polymorphisms (SNPs) of hTERT (rs2736109 G>A, rs2735940 T>C, rs2853669 A>G and rs2736100 T>G) on susceptibility to gastric cancer (GC) in Turkish population. The genotype frequency of hTERT rs2736109 G>A, rs2735940 T>C, rs2853669 A>G and rs2736100 T>G polymorphisms were determined by using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and TaqMan methods in 104 subjects with GC and 209 healthy control subjects. We found that hTERT rs2736109 G>A (AA+AG vs. GG OR=1.68 95% CI=1.01-2.81, P=0.04), rs2735940 T>C (CC vs. CT+TT: OR=2.53 95% CI=1.01-6.13, P=0.03), and rs2736100 T>G (TT vs. TG+GG: OR=2.27 95% CI=1.23-4.17, P=0.006) polymorphisms were associated with risk of GC. In the haplotype analysis, hTERT Grs2736109/Trs2735940/Ars2853669/Grs2736100 haplotype was also related with an increased risk of GC (OR=1.75; 95% CI: 1.05-2.93, P=0.03). Because this is the first study regarding the hTERT rs2736109 G>A, rs2735940 T>C, rs2853669 A>G and rs2736100 T>G polymorphisms and the risk of GC susceptibility in the literature, further independent studies are needed to verify our results in a larger sample sizes, as well as in patients of different populations.
authors.affiliation: Adıyaman University, Adıyaman School of Health, Department of Nursing, 02040 Adıyaman, Turkey. Electronic address: slymnbyrm81@gmail.com.
authors.forename: Süleyman
authors.initials: S
authors.lastname: Bayram
authors.affiliation: Adıyaman University, Education and Research Hospital, Department of Gastroenterology, 02040 Adıyaman, Turkey.
authors.forename: Yakup
authors.initials: Y
authors.lastname: Ülger
authors.affiliation: Başkent University, Faculty of Medicine, Department of Medical Oncology, 01250 Adana, Turkey.
authors.forename: Ahmet Taner
authors.initials: AT
authors.lastname: Sümbül
authors.affiliation: Isparta State Hospital, Department of Gastroenterology, 32100 Isparta, Turkey.
authors.forename: Berrin Yalinbaş
authors.initials: BY
authors.lastname: Kaya
authors.affiliation: Adıyaman Univesity, Vocational School of Health Services, 02040 Adıyaman, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Genç
authors.affiliation: Adıyaman University, Faculty of Science and Letters, Department of Biology, 02040 Adıyaman, Turkey.
authors.forename: Eyyüp
authors.initials: E
authors.lastname: Rencüzoğullari
authors.affiliation: Adıyaman Univesity, Faculty of Medicine, Department of Thoracic Surgery, 02040 Adıyaman, Turkey.
authors.forename: Erdoğan
authors.initials: E
authors.lastname: Dadaş
mesh_terms: D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D013274:Stomach Neoplasms / Q000235:genetics*; D019098:Telomerase / Q000235:genetics*; D016615:Telomere / Q000235:genetics; D014421:Turkey
chemical_list: C509186:TERT protein, human; D019098:Telomerase
keywords: Gastric cancer; Genetic susceptibility; Telomerase reverse transcriptase; hTERT rs2736109 G>A; rs2735940 T>C; rs2853669 A>G and rs2736100 T>G polymorphisms