title: Recombinant protein production data after expression in the bacterium Escherichia coli.
abstract: Fusion proteins have become essential for the expression and purification of recombinant proteins in Escherichia coli. The metal-binding protein CusF has shown several features that make it an attractive fusion protein and affinity tag: "Expression and purification of recombinant proteins in Escherichia coli tagged with the metal-binding protein CusF" (Cantu-Bustos et al., 2016 [1]). Here we present accompanying data from protein expression experiments; we tested different protein tags, temperatures, expression times, cellular compartments, and concentrations of inducer in order to obtain soluble protein and low formation of inclusion bodies. Additionally, we present data from the purification of the green fluorescent protein (GFP) tagged with CusF, using Ag(I) metal affinity chromatography.
authors.affiliation: Universidad Autonoma de Nuevo Leon, Facultad de Ciencias Quimicas, Av. Universidad s/n, Ciudad Universitaria, San Nicolas de los Garza, Nuevo Leon 66451, Mexico.
authors.forename: J Enrique
authors.initials: JE
authors.lastname: Cantu-Bustos
authors.affiliation: Universidad Autonoma de Nuevo Leon, Facultad de Ciencias Quimicas, Av. Universidad s/n, Ciudad Universitaria, San Nicolas de los Garza, Nuevo Leon 66451, Mexico.
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Cano Del Villar
authors.affiliation: Universidad Autonoma de Nuevo Leon, Facultad de Ciencias Quimicas, Av. Universidad s/n, Ciudad Universitaria, San Nicolas de los Garza, Nuevo Leon 66451, Mexico.
authors.forename: Teresa
authors.initials: T
authors.lastname: Vargas-Cortez
authors.affiliation: Universidad Autonoma de Nuevo Leon, Facultad de Ciencias Quimicas, Av. Universidad s/n, Ciudad Universitaria, San Nicolas de los Garza, Nuevo Leon 66451, Mexico.
authors.forename: Jose Ruben
authors.initials: JR
authors.lastname: Morones-Ramirez
authors.affiliation: Universidad Autonoma de Nuevo Leon, Facultad de Ciencias Quimicas, Av. Universidad s/n, Ciudad Universitaria, San Nicolas de los Garza, Nuevo Leon 66451, Mexico.
authors.forename: Isaias
authors.initials: I
authors.lastname: Balderas-Renteria
authors.affiliation: Universidad Autonoma de Nuevo Leon, Facultad de Ciencias Quimicas, Av. Universidad s/n, Ciudad Universitaria, San Nicolas de los Garza, Nuevo Leon 66451, Mexico.
authors.forename: Xristo
authors.initials: X
authors.lastname: Zarate
keywords: Affinity tag; CusF; Escherichia coli; Fusion protein; GST