title: Mass spectrometry-based monitoring of millisecond protein-ligand binding dynamics using an automated microfluidic platform.
abstract: Characterizing protein-ligand binding dynamics is crucial for understanding protein function and for developing new therapeutic agents. We present a novel microfluidic platform that features rapid mixing of protein and ligand solutions, variable incubation times, and an integrated electrospray ionization source for mass spectrometry-based monitoring of protein-ligand binding dynamics. This platform offers many advantages, including solution-based binding, label-free detection, automated operation, rapid mixing, and low sample consumption.
authors.affiliation: Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99352, USA. ryan.kelly@pnnl.gov.
authors.forename: Yongzheng
authors.initials: Y
authors.lastname: Cong
authors.affiliation: Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99352, USA. ryan.kelly@pnnl.gov.
authors.forename: Shanta
authors.initials: S
authors.lastname: Katipamula
authors.affiliation: Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99352, USA. ryan.kelly@pnnl.gov.
authors.forename: Cameron D
authors.initials: CD
authors.lastname: Trader
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99352, USA.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Orton
authors.affiliation: Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99352, USA. ryan.kelly@pnnl.gov.
authors.forename: Tao
authors.initials: T
authors.lastname: Geng
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99352, USA.
authors.forename: Erin S
authors.initials: ES
authors.lastname: Baker
authors.affiliation: Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99352, USA. ryan.kelly@pnnl.gov.
authors.forename: Ryan T
authors.initials: RT
authors.lastname: Kelly
mesh_terms: D000465:Algorithms; D057205:Automation, Laboratory; D024401:Carbonic Anhydrase I / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D002257:Carbonic Anhydrase Inhibitors / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D004058:Diffusion; D004232:Diuretics / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D004867:Equipment Design; D005665:Furosemide / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D056656:Lab-On-A-Chip Devices; D008024:Ligands; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D002257:Carbonic Anhydrase Inhibitors; D004232:Diuretics; D008024:Ligands; D005665:Furosemide; D024401:Carbonic Anhydrase I