title: Praziquantel induced oxidative stress and apoptosis-like cell death in Raillietina echinobothrida.
abstract: Praziquantel (PZQ) is an anthelmintic drug used against trematode and cestode parasites of humans and veterinary animals. Since praziquantel was introduced as a broadspectrum anthelmintic, numerous studies described its successful use against helminth parasites, but its exact mechanism of action is feebly understood. Therefore, the present study was carried out to evaluate the possible role of PZQ induced oxidative stress in apoptosis-like cell death in the poultry tapeworm Raillietina echinobothrida. Parasite viability assay revealed a time-dependent reduction in the worm viability compared to the control. Transmission electron microscopy showed typical apoptotic features like condensed nucleus, damaged nuclear envelope and altered mitochondrial membrane in PZQ exposed parasites. Results revealed chromatin condensation and DNA fragmentation in PZQ exposed parasites. There was a notable decline in the level of glutathione and glutathione-s-transferase activity leading to the augmented generation of reactive oxygen species. This led to the alterations in the mitochondrial membrane potential with increased active caspase-3/7, confirms the involvement of mitochondria in the event. The present study suggests that PZQ exerts oxidative stress leading to apoptosis-like events in the parasites resulting their death.
authors.affiliation: Parasitology Laboratory, Department of Zoology, North-Eastern Hill University, Shillong 793022, Meghalaya, India.
authors.forename: Bikash Ranjan
authors.initials: BR
authors.lastname: Giri
authors.affiliation: Parasitology Laboratory, Department of Zoology, North-Eastern Hill University, Shillong 793022, Meghalaya, India. Electronic address: bishnuroy12@rediffmail.com.
authors.forename: Bishnupada
authors.initials: B
authors.lastname: Roy
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002589:Cestoda / Q000187:drug effects*; D002590:Cestode Infections / Q000517:prevention & control; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology; D011200:Poultry / Q000469:parasitology*; D011201:Poultry Diseases / Q000517:prevention & control; D011223:Praziquantel / Q000494:pharmacology*
chemical_list: D000871:Anthelmintics; D010936:Plant Extracts; D011223:Praziquantel
keywords: Apoptosis; Praziquantel; Raillietina echinobothrida; Reactive oxygen species