title: PAX3+skeletal muscle satellite cells retain long-term self-renewal and proliferation.
abstract: Different populations of satellite cells (SCs) have been identified, but their functional difference remains unclear.
  
  
  
  We used cell-surface markers and paired box transcription factor 3 (Pax3)/paired box transcription factor 7 (Pax7) expression to separate SC populations. In addition, self-renewal, proliferation, and differentiation abilities of each population were analyzed.
  
  
  
  Pax3+/Pax7-SCs exhibited higher proliferation ability characterized by forming clusters of myogenic colonies with more self-renewing cells after several passages, while Pax3-/Pax7+SCs had faster differentiation. The myotubes derived from Pax3+/Pax7-SCs tended to express slow-myosin heavy chain and exhibited rhythmic contraction, while myotubes originating from Pax3-/Pax7+SCs primarily formed fast-myosin heavy chains characterized by transitory contraction.
  
  
  
  Pax3+/Pax7-SCs exhibited the ability of long-term self-renewal and proliferation, whereas Pax3-/Pax7+SCs demonstrated faster differentiation. Muscle Nerve 54: 943-951, 2016.
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No. 46, Xinkang Road, Yaan, Sichuan, 625014, People's Republic of China.
authors.forename: Qiumei
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No. 46, Xinkang Road, Yaan, Sichuan, 625014, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yu
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No. 46, Xinkang Road, Yaan, Sichuan, 625014, People's Republic of China.
authors.forename: Bing
authors.initials: B
authors.lastname: Yu
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No. 46, Xinkang Road, Yaan, Sichuan, 625014, People's Republic of China.
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No. 46, Xinkang Road, Yaan, Sichuan, 625014, People's Republic of China.
authors.forename: Keying
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No. 46, Xinkang Road, Yaan, Sichuan, 625014, People's Republic of China.
authors.forename: De
authors.lastname: Wu
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No. 46, Xinkang Road, Yaan, Sichuan, 625014, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: He
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No. 46, Xinkang Road, Yaan, Sichuan, 625014, People's Republic of China.
authors.forename: Xiangbing
authors.initials: X
authors.lastname: Mao
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No. 46, Xinkang Road, Yaan, Sichuan, 625014, People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zheng
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No. 46, Xinkang Road, Yaan, Sichuan, 625014, People's Republic of China. chendw@sicau.edu.cn.
authors.forename: Daiwen
authors.initials: D
authors.lastname: Chen
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000950:Antigens, Ly / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004622:Embryo, Mammalian; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D024510:Muscle Development / Q000502:physiology*; D009135:Muscular Diseases / Q000473:pathology / Q000503:physiopathology; D017570:MyoD Protein / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000378:metabolism; D000072041:PAX3 Transcription Factor / Q000378:metabolism*; D051763:PAX7 Transcription Factor / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000378:metabolism; D032496:Satellite Cells, Skeletal Muscle / Q000502:physiology*
chemical_list: D000950:Antigens, Ly; C514936:CXCR4 protein, mouse; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D017570:MyoD Protein; D000072041:PAX3 Transcription Factor; D051763:PAX7 Transcription Factor; D019718:Receptors, CXCR4; C068686:Pax3 protein, mouse; D017493:Leukocyte Common Antigens; D018995:Myosin Heavy Chains
keywords: Pax3; Pax7; differentiation; proliferation; satellite cells; self-renewal