title: 5α-Dihydrotestosterone enhances wound healing in diabetic rats.
abstract: Wound healing involves a complex interaction between the cells, extracellular matrix and oxidative response.
  
  
  OBJECTIVE
  Analyze the effects of 5α-Dihydrotestosterone (5α-DTH) ointment in cutaneous wound healing by secondary intention in diabetic Wistar rats.
  
  
  METHODS
  Rats (302.23±26.23g, n=48) were maintained in cages with food and water ad libitum in accordance with the Guiding Principles in the Use of Animal Ethics Committee. Diabetes was induced by intraperitoneal injection of streptozotocin (60mg/kg). Three skin wounds (12mm diameter) were created on the animals' back, which were randomized into 6 groups according to the application received: VT group: Vehicle (lanolin), SA group: 0.9% saline solution, NC group: Non-diabetic, CP group: positive control (silver sulfadiazine 0001%), T1 group: Testosterone (10%), T2 group: Testosterone (20%) emulsified in lanolin. The applications were made daily within 21days, and tissues from different wounds were removed every 7days.
  
  
  RESULTS
  Both groups treated with testosterone (T1 and T2) showed a significantly higher proportion of type I and type III collagen fibers. Superoxide dismutase levels were significantly higher on days 7 and 14 in testosterone treated groups. Protein carbonyls and MDA were lower in both groups.
  
  
  CONCLUSIONS
  We conclude that groups treated with 5α-DTH showed a better healing pattern with complete wound closure, and proved to have a positive effect on the morphology of the scar tissue as well as an antioxidant stimulating effect during secondhand intention skin wounds repair in diabetic rats.
authors.affiliation: Department of Animal Biology, Federal University of Viçosa, Viçosa, MG, Brazil. Electronic address: reggiani.goncalves@ufv.br.
authors.forename: Reggiani V
authors.initials: RV
authors.lastname: Gonçalves
authors.affiliation: Institute of Biomedical Sciences, Department of Structural Biology, Federal University of Alfenas, Alfenas, MG, Brazil.
authors.forename: Rômulo D
authors.initials: RD
authors.lastname: Novaes
authors.affiliation: Department of General Biology, Federal University of Viçosa, Viçosa, MG, Brazil.
authors.forename: Mariáurea M
authors.initials: MM
authors.lastname: Sarandy
authors.affiliation: Department of General Biology, Federal University of Viçosa, Viçosa, MG, Brazil.
authors.forename: Eduardo M
authors.initials: EM
authors.lastname: Damasceno
authors.affiliation: Department of General Biology, Federal University of Viçosa, Viçosa, MG, Brazil.
authors.forename: Sérgio L P
authors.initials: SL
authors.lastname: da Matta
authors.affiliation: Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlandia, MG, Brazil.
authors.forename: Neire M
authors.initials: NM
authors.lastname: de Gouveia
authors.affiliation: Department of Animal Biology, Federal University of Viçosa, Viçosa, MG, Brazil.
authors.forename: Mariella B
authors.initials: MB
authors.lastname: Freitas
authors.affiliation: Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlandia, MG, Brazil.
authors.forename: Foued S
authors.initials: FS
authors.lastname: Espindola
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002921:Cicatrix / Q000473:pathology; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D013196:Dihydrotestosterone / Q000494:pharmacology* / Q000627:therapeutic use; D004339:Drug Compounding; D008297:Male; D008407:Mast Cells / Q000187:drug effects; D050050:Protein Carbonylation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000293:injuries; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D024042:Collagen Type I; D024061:Collagen Type III; D013196:Dihydrotestosterone
keywords: 5α-Dihydrotestosterone; Antioxidant effect; Diabetes; Mast cells; Skin stereological analysis; wound contraction