title: Effect of DSPE-PEG on compound action potential, injury potential and ion concentration following compression in ex vivo spinal cord.
abstract: It has been shown that polyethylene glycol (PEG) can reseal membrane disruption on the spinal cord, but only high concentrations of PEG have been shown to have this effect. Therefore, the effect of PEG is somewhat limited, and it is necessary to investigate a new approach to repair spinal cord injury. This study assesses the ability of 1, 2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(poly (ethylene glycol)) 2000] (DSPE-PEG) to recover physiological function and attenuate the injury-induced influx of extracellular ions in ex vivo spinal cord injury. Isolated spinal cords were subjected to compression injury and treated with PEG or DSPE-PEG immediately after injury. The compound action potential (CAP) was recorded before and after injury to assess the functional recovery. Furthermore, injury potential, the difference in gap potentials before and after compression, and the concentration of intracellular ions were used to evaluate the effect of DSPE-PEG on reducing ion influx. Data showed that the injury potential and ion concentration of the untreated, PEG and DSPE-PEG group, without significant difference among them, are remarkably higher than those of the intact group. Moreover, the CAP recovery of the DSPE-PEG and PEG treated spinal cords was significantly greater than that of the untreated spinal cords. The level of CAP recovery in the DSPE-PEG and PEG treated groups was the same, but the concentration of DSPE-PEG used was much lower than the concentration of PEG. These results suggest that instant application of DSPE-PEG could effectively repair functional disturbance in SCI at a much lower concentration than PEG.
authors.affiliation: Institute of Electrical Engineering, Chinese Academy of Sciences, No. 6 Beiertiao, Zhongguancun, 100190 Beijing, China; University of Chinese Academy of Sciences, No. 19A Yuquan Road, 100049 Beijing, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Wang
authors.affiliation: Institute of Electrical Engineering, Chinese Academy of Sciences, No. 6 Beiertiao, Zhongguancun, 100190 Beijing, China. Electronic address: huoxl@mail.iee.ac.cn.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Huo
authors.affiliation: Institute of Electrical Engineering, Chinese Academy of Sciences, No. 6 Beiertiao, Zhongguancun, 100190 Beijing, China.
authors.forename: Guanghao
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Institute of Electrical Engineering, Chinese Academy of Sciences, No. 6 Beiertiao, Zhongguancun, 100190 Beijing, China; University of Chinese Academy of Sciences, No. 19A Yuquan Road, 100049 Beijing, China.
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Electrical Engineering, Chinese Academy of Sciences, No. 6 Beiertiao, Zhongguancun, 100190 Beijing, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Institute of Electrical Engineering, Chinese Academy of Sciences, No. 6 Beiertiao, Zhongguancun, 100190 Beijing, China.
authors.forename: Changzhe
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Orthopedics, Beijing Tsinghua Chang Gung Hospital, Tsinghua University, No. 168 Litang Road, 102218 Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Rong
authors.affiliation: National Center for Nanoscience and Technology, Chinese Academy of Sciences, No.11 Beiyitiao, Zhongguancun, 100190 Beijing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.affiliation: Institute of Electrical Engineering, Chinese Academy of Sciences, No. 6 Beiertiao, Zhongguancun, 100190 Beijing, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Song
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002413:Cations, Divalent; D002414:Cations, Monovalent; D005260:Female; D008564:Membrane Potentials / Q000187:drug effects*; D010714:Phosphatidylethanolamines / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000378:metabolism*; D013117:Spinal Cord Compression / Q000378:metabolism* / Q000503:physiopathology*
chemical_list: C519184:1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy-poly(ethylene glycol 2000); D002413:Cations, Divalent; D002414:Cations, Monovalent; D010714:Phosphatidylethanolamines; D011092:Polyethylene Glycols; D012964:Sodium; D002118:Calcium
keywords: Compound action potential; Double sucrose gap technique; Injury potential; Ion influx; Membrane sealing; Spinal cord injury