title: Construction and Immunogenicity of Recombinant Swinepox Virus Expressing Outer Membrane Protein L of Salmonella.
abstract: Salmonella spp. are gram-negative flagellated bacteria that cause a variety of diseases in humans and animals, ranging from mild gastroenteritis to severe systemic infection. To explore development of a potent vaccine against Salmonella infections, the gene encoding outer membrane protein L (ompL) was inserted into the swinepox virus (SPV) genome by homologous recombination. PCR, western blot, and immunofluorescence assays were used to verify the recombinant swinepox virus rSPV-OmpL. The immune responses and protection efficacy of rSPV-OmpL were assessed in a mouse model. Forty mice were assigned to four groups, which were immunized with rSPV-OmpL, inactive Salmonella (positive control), wildtype SPV (wtSPV; negative control), or PBS (challenge control), respectively. The OmpLspecific antibody in the rSPV-OmpL-immunized group increased dramatically and continuously over time post-vaccination, and was present at a significantly higher level than in the positive control group (p < 0.05). The concentrations of IFN-γ and IL-4, which represent Th1-type and Th2-type cytokine responses, were significantly higher (p < 0.05) in the rSPVOmpL- vaccinated group than in the other three groups. After intraperitoneal challenge with a lethal dose of Salmonella typhimurium CVCC542, eight out of ten mice in the rSPV-OmpLvaccinated group were protected, whereas all the mice in the negative control and challenge control groups died within 3 days. Passive immune protection assays showed that hyperimmune sera against OmpL could provide mice with effective protection against challenge from S. typhimurium. The recombinant swinepox virus rSPV-OmpL might serve as a promising vaccine against Salmonella infection.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R. China.
authors.forename: Yizhen
authors.initials: Y
authors.lastname: Fang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R. China.
authors.forename: Huixing
authors.initials: H
authors.lastname: Lin
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R. China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Ma
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R. China.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, P.R. China.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Fan
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000276:immunology*; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D023061:Gene Order; D005822:Genetic Vectors / Q000235:genetics*; D051379:Mice; D010802:Phylogeny; D012475:Salmonella / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D012480:Salmonella Infections / Q000276:immunology / Q000382:microbiology / Q000401:mortality; D018148:Suipoxvirus / Q000235:genetics*; D013552:Swine; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D016207:Cytokines
keywords: Recombinant swinepox virus; Salmonella; outer membrane protein L; vaccine