title: CD56(dim)CD16(high) and CD56(bright)CD16(-) cell percentages associated with maximum knee extensor strength and incidence of death in elderly.
abstract: Physical fitness is an indicator of systemic well-being in humans. Little is known about the role of physical fitness for maintaining systemic health in the elderly. Here, we study elderly subjects to determine the relationships between physical fitness and CD56 and CD16 surface NK cell markers on peripheral blood lymphocytes, as well as to analyze the relationship between the surface markers and incidence of death. We selected 253 independent elderly subjects (122 female; 131 male) who were 79-80 years old. Subjects having a higher proportion of CD56(dim)CD16(high) within CD56(+)CD16(+) cells, or ration of CD56(dim)CD16(high) and CD56(dim)CD16(-) cells had a significant positive correlation with maximum bilateral knee extensor strength/weight (kg) (r = 0.425; P < 0.0001 or r = 0.323; P < 0.0001). In contrast, an increased proportion of CD56(bright)CD16(-) cells within lymphocyte significantly negatively correlated with the maximum bilateral knee extensor strength/weight (kg) (r = -0.290; P = 0.0004); and these subjects had a significantly lower mortality during the 5 years following measurement of death. Therefore, we found that a synergistic effect of the right and left leg muscle strength was associated with proportion of matured NK and NKT cells and induced a low proportion of CD56(bright)CD16(-) cells within lymphocyte. Moreover, the low proportion of CD56(bright)CD16(-) cells was associated with incidence of death. In conclusion, measurements of physical fitness, the proportion of CD56(dim)CD16(high) within CD56(+)CD16(+) cells, the ratio of CD56(dim)CD56(high) and CD56(dim)CD16(-) cells, and the proportion of CD56(bright)C16(-) cells in lymphocytes are important indicators to check elderly health.
authors.affiliation: Department of Bacteriology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640 Japan.
authors.forename: Hidenobu
authors.initials: H
authors.lastname: Senpuku
authors.affiliation: Department of Oral Health Science, Graduate School of Medical and Dental Science, Niigata University, Niigata, Niigata 950-2181 Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Miyazaki
authors.affiliation: Department of Oral Health Science, Graduate School of Medical and Dental Science, Niigata University, Niigata, Niigata 950-2181 Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Yoshihara
authors.affiliation: Department of Bacteriology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640 Japan.
authors.forename: Saori
authors.initials: S
authors.lastname: Yoneda
authors.affiliation: Department of Bacteriology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640 Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Narisawa
authors.affiliation: Department of Bacteriology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640 Japan.
authors.forename: Taketo
authors.initials: T
authors.lastname: Kawarai
authors.affiliation: Laboratory of Physical Education, Sports Management Research Center, School of Information-oriented Management, SANNO University, Setagarya-ku, Tokyo 158-8630 Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Nakagawa
authors.affiliation: Department of Health Promotion and Exercise, National Institute of Health and Nutrition, Shinjuku-ku, Tokyo 162-8640 Japan.
authors.forename: Motohiko
authors.initials: M
authors.lastname: Miyachi
authors.affiliation: Department of Health Science, Hyogo University, Kakogawa, Hyogo 675-0101 Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Tada
authors.affiliation: National Institute of Fitness and Sports in Kanoya, Kanoya, Kagoshima 891-2311 Japan.
authors.forename: Goichiro
authors.initials: G
authors.lastname: Yoshida
authors.affiliation: Laboratory of Physical Education, Chiba College of Health Science, Chiba, Chiba 261-0014 Japan.
authors.forename: Mieko
authors.initials: M
authors.lastname: Shimada
authors.affiliation: Faculty of Education and Human Science Physical Education, Health and Sport Science, Institute of Humanities, Social Science and Education, Niigata University, Niigata, Niigata 950-2181 Japan.
authors.forename: Masaharu
authors.initials: M
authors.lastname: Ohashi
authors.affiliation: Laboratory of Physical Education, Chiba College of Health Science, Chiba, Chiba 261-0014 Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Nishimuta
authors.affiliation: Faculty of Culture and Education, Saga University, Saga, Saga 840-8502 Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Kimura
authors.affiliation: National Institute of Fitness and Sports in Kanoya, Kanoya, Kagoshima 891-2311 Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yoshitake
keywords: CD56brightCD16− cell; CD56dimCD16high cell; Elderly; Maximum bilateral knee extensor strength; NK cell; Physical fitness