title: Curcumin reduces brain-infiltrating T lymphocytes after intracerebral hemorrhage in mice.
abstract: T lymphocytes contribute to inflammation, thereby exacerbating neuronal injury after cerebral ischemia. An increasing amount of evidence indicates that inflammation is a key contributor to intracerebral hemorrhage (ICH)-induced secondary brain injury. Curcumin, a low-molecular-weight curry spice that is derived from the Curcuma longa plant, suppresses T lymphocyte proliferation and migration. Based on these findings, we investigated whether treatment with curcumin would reduce the number of cerebral T lymphocytes in mice with experimentally induced ICH. We found that a large number of T lymphocytes infiltrated the brain at 3days post-ICH. Curcumin significantly improved neurological scores and reduced brain edema in mice with ICH, consistent with a role in reducing neuroinflammation and neurovascular injury. Using flow cytometry, we observed significantly fewer T lymphocytes in brain samples obtained from the curcumin-treated group than in samples obtained from the vehicle-treated group. Moreover, Western blot analysis and immunostaining indicated that treatment with curcumin significantly reduced the expression of a vascular cell adhesion molecule-1 (VCAM-1), interferon-γ (INF-γ) and interleukin-17 (IL-17) in the mouse brain at 72h post-ICH. Our results suggest that administering curcumin may alleviate cerebral inflammation resulting from ICH, at least in part by reducing the infiltration of T lymphocytes into the brain. Therefore, preventing T lymphocytes from infiltrating the brain may become a new strategy for treating clinical ICH.
authors.affiliation: Department of Neurosurgery, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China.
authors.forename: Jichao
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Department of Neurosurgery, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Neurosurgery, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Liao
authors.affiliation: Department of Neurosurgery, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China.
authors.forename: Zexian
authors.initials: Z
authors.lastname: Wen
authors.affiliation: Department of Neurosurgery, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China.
authors.forename: Yaxing
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Feng
authors.affiliation: Department of Neurosurgery, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China. Electronic address: jklin@tmmu.edu.cn.
authors.forename: Jiangkai
authors.initials: J
authors.lastname: Lin
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D001929:Brain Edema / Q000188:drug therapy; D002543:Cerebral Hemorrhage / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D003474:Curcumin / Q000494:pharmacology* / Q000627:therapeutic use; D004660:Encephalitis / Q000188:drug therapy / Q000276:immunology / Q000473:pathology; D007371:Interferon-gamma / Q000378:metabolism; D020381:Interleukin-17 / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
chemical_list: D020381:Interleukin-17; D019010:Vascular Cell Adhesion Molecule-1; D007371:Interferon-gamma; D003474:Curcumin
keywords: Curcumin; Inflammation; Intracerebral hemorrhage; T lymphocytes