title: CXCR2-Mediated Granulocytic Myeloid-Derived Suppressor Cells' Functional Characterization and Their Role in Maternal Fetal Interface.
abstract: To investigate CXC chemokine receptor 2 (CXCR2)-mediated granulocytic myeloid-derived suppressor cells' (MDSCs) (G-MDSCs) functional characterization and their role in maternal-fetal interface. Proportions of CXCR2(+) MDSCs and CXCR2 protein levels in total MDSCs were lower in abortion-prone CBA/J×DBA/2 mice than in CBA/J×BALB/c mice with normal pregnancy. Treatment with CXCR2 neutralizing antibody in vivo at early stage of pregnancy significantly increased the embryo resorption rates and reduced MDSCs abundance in mice from CBA/J×BALB/c matings. Adoptive transfer of MDSCs improved pregnancy outcomes in anti-CXCR2-pretreated CBA/J mice in CBA/J×BALB/C matings. CXCR2 was capable of enhancing the migration of G-MDSCs efficiently instead of monocytic MDSCs (M-MDSCs). In addition to preferential G-MDSC accumulation, arginase I expression as well as arginase I activity of G-MDSCs were regulated by CXCR2. CXCL1, as one of CXCR2 ligands, correlated well with CXCR2-mediated G-MDSCs migration and arginase I activity. CXCR2/CXCL1 axis promotes G-MDSC recruitment and facilitates arginase I expression and activity of these cells at maternal-fetal interface. These findings provide comprehensive insights into how G-MDSCs are recruited to decidual tissues and how local G-MDSCs maintain pregnancy tolerance.
authors.affiliation: 1 Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai JiaoTong University , Shanghai, People's Republic of China .|2 Shanghai Key Laboratory of Gynecologic Oncology , Shanghai, People's Republic of China .
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Kang
authors.affiliation: 1 Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai JiaoTong University , Shanghai, People's Republic of China .|2 Shanghai Key Laboratory of Gynecologic Oncology , Shanghai, People's Republic of China .
authors.forename: Xiaoxin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: 1 Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai JiaoTong University , Shanghai, People's Republic of China .|2 Shanghai Key Laboratory of Gynecologic Oncology , Shanghai, People's Republic of China .
authors.forename: Zhilan
authors.initials: Z
authors.lastname: Liu
authors.affiliation: 1 Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai JiaoTong University , Shanghai, People's Republic of China .|2 Shanghai Key Laboratory of Gynecologic Oncology , Shanghai, People's Republic of China .
authors.forename: Haijing
authors.initials: H
authors.lastname: Xu
authors.affiliation: 1 Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai JiaoTong University , Shanghai, People's Republic of China .|2 Shanghai Key Laboratory of Gynecologic Oncology , Shanghai, People's Republic of China .
authors.forename: Tongfei
authors.initials: T
authors.lastname: Wang
authors.affiliation: 1 Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai JiaoTong University , Shanghai, People's Republic of China .|2 Shanghai Key Laboratory of Gynecologic Oncology , Shanghai, People's Republic of China .
authors.forename: Liying
authors.initials: L
authors.lastname: He
authors.affiliation: 1 Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai JiaoTong University , Shanghai, People's Republic of China .|2 Shanghai Key Laboratory of Gynecologic Oncology , Shanghai, People's Republic of China .
authors.forename: Aimin
authors.initials: A
authors.lastname: Zhao
mesh_terms: D000818:Animals; D054360:Chemokine CXCL1 / Q000378:metabolism*; D005260:Female; D007108:Immune Tolerance / Q000276:immunology*; D033261:Maternal-Fetal Relations / Q000502:physiology*; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D022423:Myeloid Cells / Q000276:immunology / Q000378:metabolism*; D000072737:Myeloid-Derived Suppressor Cells / Q000378:metabolism*; D011247:Pregnancy; D023063:Receptors, Interleukin-8B / Q000378:metabolism*
chemical_list: D054360:Chemokine CXCL1; C475895:Cxcl1 protein, mouse; D023063:Receptors, Interleukin-8B