title: Feasibility of using DNA-immobilized nanocellulose-based immunoadsorbent for systemic lupus erythematosus plasmapheresis.
abstract: The goal of this project was to study the feasibility of using a DNA-immobilized nanocellulose-based immunoadsorbent for possible application in medical apheresis such as systemic lupus erythematosus (SLE) treatment. Calf thymus DNA was bound to high surface area nanocellulose membrane at varying concentrations using UV-irradiation. The DNA-immobilized samples were characterized with scanning electron microscopy, atomic force microscopy, and phosphorus elemental analysis. The anti-ds-DNA IgG binding was tested in vitro using ELISA. The produced sample showed high affinity in vitro to bind anti-ds-DNA-antibodies from mice, as much as 80% of added IgG was bound by the membrane. Furthermore, the binding efficiency was quantitatively dependent on the amount of immobilized DNA onto nanocellulose membrane. The described nanocellulose membranes are interesting immunoadsorbents for continued clinical studies.
authors.affiliation: Nanotechnology and Functional Materials, Department of Engineering Sciences, Uppsala University, Box 534, 75121 Uppsala, Sweden. Electronic address: changgang.xu@angstrom.uu.se.
authors.forename: Changgang
authors.initials: C
authors.lastname: Xu
authors.affiliation: Nanotechnology and Functional Materials, Department of Engineering Sciences, Uppsala University, Box 534, 75121 Uppsala, Sweden.
authors.forename: Daniel O
authors.initials: DO
authors.lastname: Carlsson
authors.affiliation: Nanotechnology and Functional Materials, Department of Engineering Sciences, Uppsala University, Box 534, 75121 Uppsala, Sweden. Electronic address: albert.mihranyan@angstrom.uu.se.
authors.forename: Albert
authors.initials: A
authors.lastname: Mihranyan
mesh_terms: D000818:Animals; D000974:Antibodies, Antinuclear / Q000302:isolation & purification*; D002482:Cellulose / Q000737:chemistry* / Q000528:radiation effects; D004247:DNA / Q000737:chemistry*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D061925:Immobilized Nucleic Acids / Q000737:chemistry; D007074:Immunoglobulin G / Q000302:isolation & purification*; D007164:Immunosorbents / Q000737:chemistry* / Q000528:radiation effects; D008180:Lupus Erythematosus, Systemic / Q000276:immunology / Q000473:pathology / Q000628:therapy; D051379:Mice; D049329:Nanostructures / Q000737:chemistry; D010956:Plasmapheresis / Q000379:methods; D014466:Ultraviolet Rays
chemical_list: D000974:Antibodies, Antinuclear; D061925:Immobilized Nucleic Acids; D007074:Immunoglobulin G; D007164:Immunosorbents; D002482:Cellulose; D004247:DNA; C427203:calf thymus DNA
keywords: Autoimmune diseases; DNA immobilization; Immunoadsorption; Nanocellulose; Systemic lupus erythematosus