title: TET2 regulates LncRNA-ANRIL expression and inhibits the growth of human gastric cancer cells.
abstract: Ten-eleven translocation (TET) family enzymes convert 5-methylcytosine to 5-hydroxymethylcytosine in DNA. However, the role of TET enzymes in human gastric cancer remains unknown. Here, we show that TET2 mRNA and protein levels are downregulated in human gastric cancer tissues when compared with normal gastric mucosa. Low expression of TET2 predicts poor overall and disease-free survival. Furthermore, we demonstrate that TET2 inhibits the proliferation and colony formation of human gastric cancer cells by using loss-of-function and gain-of-function strategies. Overexpression of TET2 induces apoptosis in human gastric cancer cells. The mechanism study shows that TET2 binds to the promoter region of the oncogenic long noncoding RNA (lncRNA-ANRIL) and regulates the expression of ANRIL and its downstream genes (INK4a, INK4b, and ARF). Finally, we demonstrate that ANRIL knockdown blocks the effects of TET2 on gastric cancer cell proliferation and colony formation. © 2016 IUBMB Life 68(5):355-364, 2016.
authors.affiliation: Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, China.|National Clinical Research Center of Digestive Diseases, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Deng
authors.affiliation: Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, China.|National Clinical Research Center of Digestive Diseases, Beijing, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, China.|National Clinical Research Center of Digestive Diseases, Beijing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, China.|National Clinical Research Center of Digestive Diseases, Beijing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Cai
authors.affiliation: Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, China.|National Clinical Research Center of Digestive Diseases, Beijing, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Bai
authors.affiliation: Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, China.|National Clinical Research Center of Digestive Diseases, Beijing, China.
authors.forename: Zhongtao
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D004268:DNA-Binding Proteins / Q000502:physiology*; D049308:Dioxygenases; D015536:Down-Regulation; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D062085:RNA, Long Noncoding / Q000096:biosynthesis / Q000235:genetics*; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology
chemical_list: C561518:CDKN2B antisense RNA, human; D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D062085:RNA, Long Noncoding; D049308:Dioxygenases; C541081:TET2 protein, human
keywords: TET2; apoptosis; gastric cancer; lncRNA-ANRIL