title: Venturing into the New Science of Nucleases.
abstract: Gene editing with zinc finger nucleases, transcription activator-like effector nucleases, clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated proteins system, or meganucleases can, in principle, mediate any genome modification. Recent studies have shown that COL7A1 mutations in cells of patients with recessive dystrophic epidermolysis bullosa can be corrected by homology-directed DNA repair.
authors.affiliation: Univerzita Karlova v Praze 2 lekarska fakulta, Medicine, Prague, Czech Republic.
authors.forename: Markéta
authors.initials: M
authors.lastname: Tolarová
authors.affiliation: Genetic Skin Disease Group, St. John's Institute of Dermatology, London, UK.
authors.forename: John A
authors.initials: JA
authors.lastname: McGrath
authors.affiliation: Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota, USA. Electronic address: tolar003@umn.edu.
authors.forename: Jakub
authors.initials: J
authors.lastname: Tolar
mesh_terms: D024143:Collagen Type VII / Q000235:genetics*; D004260:DNA Repair; D016108:Epidermolysis Bullosa Dystrophica / Q000235:genetics*; D006801:Humans
chemical_list: D024143:Collagen Type VII