title: Acute hypoglycemic effect and phytochemical composition of Ageratina petiolaris.
abstract: BACKGROUND
  Type 2 diabetes is characterized by tissue resistance to the action of insulin combined with a relative deficiency in insulin secretion. In Mexico, medicinal plants have traditionally been used to control the disease; in this work, we investigate the hypoglycemic effect of Ageratina petiolaris, a plant used in Mexico for the treatment of diabetes.
  
  
  METHODS
  The hypoglycemic effects of aqueous and methanolic extracts prepared from aerial parts of Ageratina petiolaris in streptozotocin-nicotinamide (STZ-NA) induced diabetic rats were assessed. An oral administration of the water extract at doses of 40 and 160mg/kg and of the methanol extract at doses of 67 and 268mg/kg were evaluated. Furthermore, the water extract at 160mg/kg was evaluated under an Oral Glucose Tolerance Test.
  
  
  RESULTS
  The tested extracts were able to reduce the increase in blood glucose level at three hours after administration. l-chiro-inositol and chlorogenic acid were isolated as important constituents of the plant, they were identified in both extracts along with other constituents.
  
  
  CONCLUSIONS
  The results presented here demonstrate that the main components in the aqueous extract of Ageratina petiolaris are chlorogenic acid and l-chiro-inositol, the last one with significant hypoglycemic activity, these results support the traditional use of this plant for the treatment of type 2 diabetes.
authors.affiliation: Instituto de Química, Universidad Nacional Autónoma de México, 04510 México CD. MX., Mexico.
authors.forename: Celia
authors.initials: C
authors.lastname: Bustos-Brito
authors.affiliation: Laboratorio de Etnofarmacología, Facultad de Ciencias, Universidad Nacional Autónoma de México, 04510 México CD. MX., Mexico. Electronic address: aac@ciencias.unam.mx.
authors.forename: Adolfo
authors.initials: A
authors.lastname: Andrade-Cetto
authors.affiliation: Instituto de Química, Universidad Nacional Autónoma de México, 04510 México CD. MX., Mexico.
authors.forename: Juan David
authors.initials: JD
authors.lastname: Giraldo-Aguirre
authors.affiliation: Laboratorio de Etnofarmacología, Facultad de Ciencias, Universidad Nacional Autónoma de México, 04510 México CD. MX., Mexico.
authors.forename: Angelina Daniela
authors.initials: AD
authors.lastname: Moreno-Vargas
authors.affiliation: Instituto de Química, Universidad Nacional Autónoma de México, 04510 México CD. MX., Mexico.
authors.forename: Leovigildo
authors.initials: L
authors.lastname: Quijano
mesh_terms: D036603:Ageratina / Q000737:chemistry*; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D005951:Glucose Tolerance Test; D005905:Glyburide / Q000494:pharmacology; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000494:pharmacology*; D064209:Phytochemicals; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D007004:Hypoglycemic Agents; D064209:Phytochemicals; D010936:Plant Extracts; D005905:Glyburide
keywords: Ageratina petiolaris; Hypoglycemic effect; Medicinal plants; Stz-NA rat; Type 2 diabetes; l-chiro-inositol