title: MiR-508-5p Inhibits the Progression of Glioma by Targeting Glycoprotein Non-metastatic Melanoma B.
abstract: Glioma is a severe and highly lethal brain cancer, a malignancy largely stemming from growing in a relatively restrained area of the brain. Hence, the understanding of the molecular regulation of the growth of glioma is critical for improving its treatment. MicroRNA has become a hotspot in research on diseases, especially in the initiation and progression of different types of cancer. However, the molecular function and mechanisms of miR-508-5p in gliomagenesis are still unclear. The aim of this study was to investigate miR-508-5p expression in glioma and determine its effects on proliferation. miR-508-5p expression levels, both in glioma cell lines and in tissue, were significantly lower than in a normal human astrocyte cell line or adjacent tissues. Cell growth was analyzed using a MTT assay and over-expression of miR-508-5p was found to decrease glioma cell growth. Moreover, a bioinformatic analysis was performed, showing that glycoprotein non-metastatic melanoma B (GPNMB) was a direct target for miR-508-5p in glioma cells. Furthermore, in vivo treatment with miR-508-5p reduced GPNMB protein levels in the tumor. Additionally, overexpression of GPNMB without 3'-UTR partially reversed the cell growth arrest induced by miR-508-5p over-expression in glioma cells. In conclusion, these results indicate that increased expression of miR-508-5p might be related to glioma progression, indicating a potential role of miR-508-5p for clinical therapy.
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yan Ta Road, Xi'an, 710061, People's Republic of China. baogangxa@sina.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Bao
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yan Ta Road, Xi'an, 710061, People's Republic of China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yan Ta Road, Xi'an, 710061, People's Republic of China.
authors.forename: Ruichun
authors.initials: R
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yan Ta Road, Xi'an, 710061, People's Republic of China.
authors.forename: Gaofeng
authors.initials: G
authors.lastname: Xu
authors.affiliation: Center for Translational Medicine, First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yan Ta Road, Xi'an, 710061, People's Republic of China.
authors.forename: Peijun
authors.initials: P
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yan Ta Road, Xi'an, 710061, People's Republic of China.
authors.forename: Baixiang
authors.initials: B
authors.lastname: He
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000517:prevention & control; D045744:Cell Line, Tumor; D018450:Disease Progression; D018390:Gene Targeting / Q000379:methods; D005910:Glioma / Q000378:metabolism* / Q000473:pathology / Q000517:prevention & control; D006801:Humans; D008562:Membrane Glycoproteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000008:administration & dosage / Q000096:biosynthesis*; D047368:Tumor Burden; D023041:Xenograft Model Antitumor Assays / Q000379:methods
chemical_list: C503042:GPNMB protein, human; C571981:MIRN508 microRNA, human; D008562:Membrane Glycoproteins; D035683:MicroRNAs
keywords: Glioma; Glycoprotein non-metastatic melanoma B (GPNMB); Growth; MiR-508-5p; Xenograft