title: Kinetic isotope effects for fast deuterium and proton exchange rates.
abstract: By monitoring the effect of deuterium decoupling on the decay of transverse (15)N magnetization in D-(15)N spin pairs during multiple-refocusing echo sequences, we have determined fast D-D exchange rates kD and compared them with fast H-H exchange rates kH in tryptophan to determine the kinetic isotope effect as a function of pH and temperature.
authors.affiliation: Ecole Polytechinque Fédérale de Lausanne, Laboratoire de Résonance Magnétique Biomoléculaire, Batochime, CH-1015 Lausanne, Switzerland. estel.canet@epfl.ch.
authors.forename: Estel
authors.initials: E
authors.lastname: Canet
authors.forename: Daniele
authors.initials: D
authors.lastname: Mammoli
authors.forename: Pavel
authors.initials: P
authors.lastname: Kadeřávek
authors.forename: Philippe
authors.initials: P
authors.lastname: Pelupessy
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Bodenhausen
mesh_terms: D003903:Deuterium; D006863:Hydrogen-Ion Concentration; D007554:Isotopes; D007700:Kinetics; D011522:Protons; D013696:Temperature
chemical_list: D007554:Isotopes; D011522:Protons; D003903:Deuterium