title: Expression of Basophil Activation Markers in Pediatric Asthma.
abstract: BACKGROUND
  Asthma is very common in children and its diagnosis is based on clinical manifestations, which can be misdiagnosed as other respiratory diseases with similar signs and symptoms.
  
  
  OBJECTIVE
  To analyze the expression of ST2L and CD203c in the diagnosis of pediatric asthma.
  
  
  METHODS
  Basophils were purified from whole blood samples of patients and healthy controls using Ficol-Paque gradient and Basophil Isolation Kit. RNA extraction was done by RNX-Plus solution and after synthesis of cDNA, the gene expression was analyzed by means of real time PCR.
  
  
  RESULTS
  Patients expressed significantly higher levels of CD203c than healthy controls (p=0.01). Although there was an increase in the transcription level of ST2L gene in patients, the results were not statistically significant compared to those obtained from the healthy controls (p>0.05). A Specificity of 60% and a sensitivity of 73% were found using ROC curve for CD203c expression. Patients with positive family history of asthma exhibited more CD203c and ST2L expression (p<0.05).
  
  
  CONCLUSIONS
  It is proposed that determining CD203c expression by real time PCR may be an effective technique for diagnosis of pediatric asthma.
authors.affiliation: Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Masooma
authors.initials: M
authors.lastname: Abdullahi
authors.forename: Reza
authors.initials: R
authors.lastname: Ranjbaran
authors.forename: Soheaila
authors.initials: S
authors.lastname: Alyasin
authors.forename: Zeinab
authors.initials: Z
authors.lastname: Keshavarz
authors.forename: Amin
authors.initials: A
authors.lastname: Ramezani
authors.forename: Abbas
authors.initials: A
authors.lastname: Behzad-Behbahani
authors.forename: Sedigheh
authors.initials: S
authors.lastname: Sharifzadeh
mesh_terms: D001249:Asthma / Q000235:genetics* / Q000276:immunology; D001491:Basophils / Q000276:immunology / Q000378:metabolism*; D015415:Biomarkers; D016022:Case-Control Studies; D002675:Child, Preschool; D015870:Gene Expression; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D000072179:Interleukin-1 Receptor-Like 1 Protein / Q000235:genetics; D010727:Phosphoric Diester Hydrolases / Q000235:genetics; D011755:Pyrophosphatases / Q000235:genetics; D012372:ROC Curve
chemical_list: D015415:Biomarkers; C427944:ENPP3 protein, human; C079241:IL1RL1 protein, human; D000072179:Interleukin-1 Receptor-Like 1 Protein; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases