title: Crystal structure of human dendritic cell inhibitory receptor C-type lectin domain reveals the binding mode with N-glycan.
abstract: Human dendritic cell inhibitory receptor (DCIR) is a C-type lectin receptor expressed in classical dendritic cells and accepts several oligosaccharide ligands including N-glycans. Here, we report the crystal structures of human DCIR C-type lectin domains in the absence and presence of a branched N-glycan unit. The domain has a typical C-type lectin fold and two bound calcium ions. In the ligand-bound form, the disaccharide unit (GlcNAcβ1-2Man) acceptably fits the electron density map, indicating that it forms the main epitope. The recognition of the nonterminal N-glycan unit explains the relatively broad specificity of this lectin.
authors.affiliation: Structural Glycobiology Team, Systems Glycobiology Research Group, RIKEN-Max Planck Joint Research Center, RIKEN Global Research Cluster, Wako, Saitama, Japan.
authors.forename: Masamichi
authors.initials: M
authors.lastname: Nagae
authors.affiliation: Structural Glycobiology Team, Systems Glycobiology Research Group, RIKEN-Max Planck Joint Research Center, RIKEN Global Research Cluster, Wako, Saitama, Japan.
authors.forename: Akemi
authors.initials: A
authors.lastname: Ikeda
authors.affiliation: Department of Chemistry, Osaka University, Machikaneyama, Toyonaka, Osaka, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Hanashima
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba, Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Kojima
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Matsumoto
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yamamoto
authors.affiliation: Structural Glycobiology Team, Systems Glycobiology Research Group, RIKEN-Max Planck Joint Research Center, RIKEN Global Research Cluster, Wako, Saitama, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Yamaguchi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018360:Crystallography, X-Ray; D003713:Dendritic Cells / Q000378:metabolism*; D006801:Humans; D037181:Lectins, C-Type / Q000737:chemistry*; D008024:Ligands; D051379:Mice; D011134:Polysaccharides / Q000737:chemistry*; D011485:Protein Binding; D000072417:Protein Domains; D011956:Receptors, Cell Surface / Q000737:chemistry*; D016415:Sequence Alignment
chemical_list: D037181:Lectins, C-Type; D008024:Ligands; D011134:Polysaccharides; D011956:Receptors, Cell Surface
keywords: C-type lectin; N-glycan; crystal structure