title: A charge-suppressing strategy for probing protein methylation.
abstract: Methylation of arginine and lysine (RK) residues play essential roles in epigenetics and the regulation of gene expression. However, research in this area is often hindered by the lack of effective tools for probing the protein methylation. Here, we present an antibody-free strategy to capture protein methylation on RK residues by using chemical reactions to eliminate the charges on un-modified RK residues and peptide N-termini. Peptides containing methylated RK residues remain positively charged and are then enriched by strong cation exchange chromatography, followed by high-resolution mass spectrometry identification.
authors.affiliation: Ottawa Institute of Systems Biology, Department of Biochemistry, Immunology and Microbiology, Faculty of Medicine, University of Ottawa, Ontario, Canada. dfigeys@uottawa.ca.
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Ning
authors.affiliation: Ottawa Institute of Systems Biology, Department of Biochemistry, Immunology and Microbiology, Faculty of Medicine, University of Ottawa, Ontario, Canada. dfigeys@uottawa.ca.
authors.forename: Alexandra Therese
authors.initials: AT
authors.lastname: Star
authors.affiliation: Ottawa Institute of Systems Biology, Department of Biochemistry, Immunology and Microbiology, Faculty of Medicine, University of Ottawa, Ontario, Canada. dfigeys@uottawa.ca.
authors.forename: Anna
authors.initials: A
authors.lastname: Mierzwa
authors.affiliation: Ottawa Institute of Systems Biology, Department of Biochemistry, Immunology and Microbiology, Faculty of Medicine, University of Ottawa, Ontario, Canada. dfigeys@uottawa.ca.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Lanouette
authors.affiliation: Ottawa Institute of Systems Biology, Department of Biochemistry, Immunology and Microbiology, Faculty of Medicine, University of Ottawa, Ontario, Canada. dfigeys@uottawa.ca.
authors.forename: Janice
authors.initials: J
authors.lastname: Mayne
authors.affiliation: Ottawa Institute of Systems Biology, Department of Biochemistry, Immunology and Microbiology, Faculty of Medicine, University of Ottawa, Ontario, Canada. dfigeys@uottawa.ca.
authors.forename: Jean-Francois
authors.initials: JF
authors.lastname: Couture
authors.affiliation: Ottawa Institute of Systems Biology, Department of Biochemistry, Immunology and Microbiology, Faculty of Medicine, University of Ottawa, Ontario, Canada. dfigeys@uottawa.ca.
authors.forename: Daniel
authors.initials: D
authors.lastname: Figeys
mesh_terms: D000595:Amino Acid Sequence; D001120:Arginine / Q000032:analysis*; D002852:Chromatography, Ion Exchange; D008239:Lysine / Q000032:analysis*; D013058:Mass Spectrometry; D008745:Methylation; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
chemical_list: D010455:Peptides; D011506:Proteins; D001120:Arginine; D008239:Lysine