title: Plant plasma membrane aquaporins in natural vesicles as potential stabilizers and carriers of glucosinolates.
abstract: Their biodegradable nature and ability to target cells make biological vesicles potential nanocarriers for bioactives delivery. In this work, the interaction between proteoliposomes enriched in aquaporins derived from broccoli plants and the glucosinolates was evaluated. The vesicles were stored at different temperatures and their integrity was studied. Determination of glucosinolates, showed that indolic glucosinolates were more sensitive to degradation in aqueous solution than aliphatic glucosinolates. Glucoraphanin was stabilized by leaf and root proteoliposomes at 25°C through their interaction with aquaporins. An extensive hydrogen bond network, including different aquaporin residues, and hydrophobic interactions, as a consequence of the interaction between the linear alkane chain of glucoraphanin and Glu31 and Leu34 protein residues, were established as the main stabilizing elements. Combined our results showed that plasma membrane vesicles from leaf and root tissues of broccoli plants may be considered as suitable carriers for glucosinolate which stabilization can be potentially attributed to aquaporins.
authors.affiliation: Plant Nutrition Department, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), Campus de Espinardo, 30100 Murcia, Spain.
authors.forename: Maria Del Carmen
authors.initials: MDC
authors.lastname: Martínez-Ballesta
authors.affiliation: Bioinformatics and High Performance Computing Research Group (BIO-HPC), Universidad Católica San Antonio de Murcia (UCAM), 30107 Murcia, Spain.
authors.forename: Horacio
authors.initials: H
authors.lastname: Pérez-Sánchez
authors.affiliation: Food Science and Technology Department, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), Campus de Espinardo, 30100 Murcia, Spain.
authors.forename: Diego A
authors.initials: DA
authors.lastname: Moreno
authors.affiliation: Plant Nutrition Department, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), Campus de Espinardo, 30100 Murcia, Spain. Electronic address: mcarvaja@cebas.csic.es.
authors.forename: Micaela
authors.initials: M
authors.lastname: Carvajal
mesh_terms: D020346:Aquaporins / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D001937:Brassica / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D004337:Drug Carriers / Q000737:chemistry / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods; D004355:Drug Stability; D005961:Glucosinolates / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism*; D057927:Hydrophobic and Hydrophilic Interactions; D007096:Imidoesters / Q000737:chemistry / Q000378:metabolism; D008563:Membrane Lipids / Q000032:analysis; D008958:Models, Molecular; D015394:Molecular Structure; D010091:Oximes; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D011485:Protein Binding; D000072417:Protein Domains; D013454:Sulfoxides; D022161:Transport Vesicles / Q000737:chemistry / Q000378:metabolism*
chemical_list: D020346:Aquaporins; D004337:Drug Carriers; D005961:Glucosinolates; D007096:Imidoesters; D008563:Membrane Lipids; D010091:Oximes; D010940:Plant Proteins; D013454:Sulfoxides; C119494:glucoraphanin
keywords: Aquaporins; Broccoli; Glucoraphanin; Glucosinolates; Plasma membrane vesicles