title: Paper-based upconversion fluorescence resonance energy transfer biosensor for sensitive detection of multiple cancer biomarkers.
abstract: A paper-based upconversion fluorescence resonance energy transfer assay device is proposed for sensitive detection of CEA. The device is fabricated on a normal filter paper with simple nano-printing method. Upconversion nanoparticles tagged with specific antibodies are printed to the test zones on the test paper, followed by the introduction of assay antigen. Upconversion fluorescence measurements are directly conducted on the test zones after the antigen-to-antibody reactions. Furthermore, a multi-channel test paper for simultaneous detection of multiple cancer biomarkers was established by the same method and obtained positive results. The device showed high anti-interfere, stability, reproducible and low detection limit (0.89 ng/mL), moreover it is very easy to fabricate and operate, which is a promising prospect for a clinical point-of-care test.
authors.affiliation: State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun 130012, People's Republic of China.|Department of Physics, Dalian Maritime University, Dalian 116026, People's Republic of China.
authors.forename: Sai
authors.initials: S
authors.lastname: Xu
authors.affiliation: State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun 130012, People's Republic of China.
authors.forename: Biao
authors.initials: B
authors.lastname: Dong
authors.affiliation: State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun 130012, People's Republic of China.
authors.forename: Donglei
authors.initials: D
authors.lastname: Zhou
authors.affiliation: State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun 130012, People's Republic of China.
authors.forename: Ze
authors.initials: Z
authors.lastname: Yin
authors.affiliation: State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun 130012, People's Republic of China.
authors.forename: Shaobo
authors.initials: S
authors.lastname: Cui
authors.affiliation: State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun 130012, People's Republic of China.
authors.forename: Wen
authors.initials: W
authors.lastname: Xu
authors.affiliation: Department of Physics, Dalian Maritime University, Dalian 116026, People's Republic of China.
authors.forename: Baojiu
authors.initials: B
authors.lastname: Chen
authors.affiliation: State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun 130012, People's Republic of China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Song
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D015374:Biosensing Techniques; D031541:Fluorescence Resonance Energy Transfer; D057230:Limit of Detection; D046529:Microscopy, Electron, Transmission; D010209:Paper; D015203:Reproducibility of Results
chemical_list: D014408:Biomarkers, Tumor