title: Suitability assessment of a continuous process combining thermo-mechano-chemical and bio-catalytic action in a single pilot-scale twin-screw extruder for six different biomass sources.
abstract: A process has been validated for the deconstruction of lignocellulose on a pilot scale installation using six types of biomass selected for their sustainability, accessibility, worldwide availability, and differences of chemical composition and physical structure. The process combines thermo-mechano-chemical and bio-catalytic action in a single twin-screw extruder. Three treatment phases were sequentially performed: an alkaline pretreatment, a neutralization step coupled with an extraction-separation phase and a bioextrusion treatment. Alkaline pretreatment destructured the wall polymers after just a few minutes and allowed the initial extraction of 18-54% of the hemicelluloses and 9-41% of the lignin. The bioextrusion step induced the start of enzymatic hydrolysis and increased the proportion of soluble organic matter. Extension of saccharification for 24h at high consistency (20%) and without the addition of new enzyme resulted in the production of 39-84% of the potential glucose.
authors.affiliation: Laboratoire de Chimie Agro-Industrielle (LCA), Université de Toulouse, INRA, INPT, Toulouse, France. Electronic address: Virginie.Vandenbossche@ensiacet.f.
authors.forename: Virginie
authors.initials: V
authors.lastname: Vandenbossche
authors.affiliation: Laboratoire de Chimie Agro-Industrielle (LCA), Université de Toulouse, INRA, INPT, Toulouse, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Brault
authors.affiliation: Facultad de Quimica, Universidad National Autónoma de México, 04510 México D.F., Mexico.
authors.forename: Oscar
authors.initials: O
authors.lastname: Hernandez-Melendez
authors.affiliation: Laboratoire de Chimie Agro-Industrielle (LCA), Université de Toulouse, INRA, INPT, Toulouse, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Evon
authors.affiliation: Facultad de Quimica, Universidad National Autónoma de México, 04510 México D.F., Mexico.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Barzana
authors.affiliation: Laboratoire de Chimie Agro-Industrielle (LCA), Université de Toulouse, INRA, INPT, Toulouse, France.
authors.forename: Gérard
authors.initials: G
authors.lastname: Vilarem
authors.affiliation: Laboratoire de Chimie Agro-Industrielle (LCA), Université de Toulouse, INRA, INPT, Toulouse, France.
authors.forename: Luc
authors.initials: L
authors.lastname: Rigal
mesh_terms: D000863:Antacids / Q000737:chemistry / Q000494:pharmacology; D055162:Biocatalysis; D005591:Chemical Fractionation / Q000379:methods; D019245:Compressive Strength; D005947:Glucose / Q000096:biosynthesis; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry* / Q000378:metabolism; D055595:Mechanical Phenomena; D010865:Pilot Projects; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D013696:Temperature
chemical_list: D000863:Antacids; D011134:Polysaccharides; C036909:lignocellulose; C007916:hemicellulose; D008031:Lignin; D005947:Glucose
keywords: Alkaline pre-treatment; Bioextrusion; Enzymatic hydrolysis; Lignocellulosic biomass; Twin-screw extruder