title: Effect of surface-modified superparamagnetic iron oxide nanoparticles (SPIONS) on mast cell infiltration: An acute in vivo study.
abstract: Extensive use of superparamagnetic iron oxide nanoparticles (SPIONS) in theranostics prompted us to investigate the acute changes in cell morphology and function following intravenous administration of surface-modified SPIONS in a rat model. Dextran-coated (DEX) and polyethylene glycol-coated (PEG) SPIONS were synthesized and characterized, and cytocompatibility was evaluated in vitro. Haematological, histopathological, ultrastructural and oxidative stress analyses were carried out 24h post intravenous administration in vivo. In test groups, SGPT and SGOT enzymes were significantly altered when compared to saline-only controls. Anti-oxidant imbalance and lipid peroxidation were observed in all major organs. Histology revealed iron-laden Kupffer cells and macrophages in liver and lung respectively. Iron overload was observed in the convoluted tubules of the kidney. Mast cell infiltration and distribution were observed differentially in test groups. Although surface modification of SPIONS improved biocompatibility in vitro, they affected anti-oxidant and tissue nitrite levels, which greatly influenced mast cell infiltration in vivo.
authors.affiliation: Histopathology Laboratory, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, Kerala, India. Electronic address: asw@sctimst.ac.in.
authors.forename: Arumugam
authors.initials: A
authors.lastname: Sabareeswaran
authors.affiliation: Bioceramics laboratory, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, Kerala, India. Electronic address: ansareb@gmail.com.
authors.forename: Ereath Beeran
authors.initials: EB
authors.lastname: Ansar
authors.affiliation: Bioceramics laboratory, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, Kerala, India. Electronic address: varma@sctimst.ac.in.
authors.forename: Parimanathu Rama Varma
authors.initials: PR
authors.lastname: Harikrishna Varma
authors.affiliation: Division of Toxicology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, Kerala, India. Electronic address: mohanpv@sctimst.ac.in.
authors.forename: Parayanthala Vilappil
authors.initials: PV
authors.lastname: Mohanan
authors.affiliation: Tissue Culture Laboratory, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, Kerala, India. Electronic address: tvkumari@sctimst.ac.in.
authors.forename: Thrikkovil Variathu
authors.initials: TV
authors.lastname: Kumary
mesh_terms: D000818:Animals; D003911:Dextrans; D015227:Lipid Peroxidation; D008168:Lung; D058185:Magnetite Nanoparticles; D008407:Mast Cells / Q000187:drug effects* / Q000502:physiology; D010084:Oxidation-Reduction; D011092:Polyethylene Glycols; D051381:Rats
chemical_list: D003911:Dextrans; D058185:Magnetite Nanoparticles; D011092:Polyethylene Glycols
keywords: Histopathology; Kupffer cells; Mast cells; Nitrite level; SPIONS