title: Effect of long-term vitamin E and selenium supplementation on urine F2-isoprostanes, a biomarker of oxidative stress.
abstract: Cigarette smoking generates reactive oxidant species and contributes to systemic oxidative stress, which plays a role in the pathophysiology of chronic diseases. Nutrients with antioxidant properties, including vitamin E and selenium, are proposed to reduce systemic oxidative burden and thus to mitigate the negative health effects of reactive oxidant species.
  
  
  
  Our objective was to determine whether long-term supplementation with vitamin E and/or selenium reduces oxidative stress in smokers, as measured by urine 8-iso-prostaglandin F2-alpha (8-iso-PGF2α).
  
  
  
  We measured urine 8-iso-PGF2α with competitive enzyme linked immunoassay (ELISA) in 312 male current smokers after 36 months of intervention in a randomized placebo-controlled trial of vitamin E (400IU/d all rac-α-tocopheryl acetate) and/or selenium (200µg/d L-selenomethionine). We used linear regression to estimate the effect of intervention on urine 8-iso-PGF2α, with adjustments for age and race.
  
  
  
  Compared to placebo, vitamin E alone lowered urine 8-iso-PGF2α by 21% (p=0.02); there was no effect of combined vitamin E and selenium (intervention arm lower by 9%; p=0.37) or selenium alone (intervention arm higher by 8%; p=0.52).
  
  
  
  Long-term vitamin E supplementation decreases urine 8-iso-PGF2α among male cigarette smokers, but we observed little to no evidence for an effect of selenium supplementation, alone or combined with vitamin E.
authors.affiliation: Division of Nutritional Sciences, Cornell University, 209 Savage Hall, Ithaca, NY 14853, USA.
authors.forename: Kristin A
authors.initials: KA
authors.lastname: Guertin
authors.affiliation: Division of Nutritional Sciences, Cornell University, 209 Savage Hall, Ithaca, NY 14853, USA.
authors.forename: Rachael K
authors.initials: RK
authors.lastname: Grant
authors.affiliation: SWOG Statistical Center, Seattle, WA, USA.
authors.forename: Kathryn B
authors.initials: KB
authors.lastname: Arnold
authors.affiliation: Division of Nutritional Sciences, Cornell University, 209 Savage Hall, Ithaca, NY 14853, USA.
authors.forename: Lindsay
authors.initials: L
authors.lastname: Burwell
authors.affiliation: SWOG Statistical Center, Seattle, WA, USA.
authors.forename: JoAnn
authors.initials: J
authors.lastname: Hartline
authors.affiliation: SWOG Statistical Center, Seattle, WA, USA.
authors.forename: Phyllis J
authors.initials: PJ
authors.lastname: Goodman
authors.affiliation: Division of Cancer Prevention, National Cancer Institute, Bethesda, MD, USA.
authors.forename: Lori M
authors.initials: LM
authors.lastname: Minasian
authors.affiliation: University of California San Diego Cancer Center, San Diego, CA, USA.
authors.forename: Scott M
authors.initials: SM
authors.lastname: Lippman
authors.affiliation: Cleveland Clinic, Cleveland, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Klein
authors.affiliation: Division of Nutritional Sciences, Cornell University, 209 Savage Hall, Ithaca, NY 14853, USA; Department of Healthcare Policy and Research, Division of Biostatistics and Epidemiology, Weill Cornell Medical College, New York, NY, USA. Electronic address: pac6@cornell.edu.
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Cassano
mesh_terms: D000368:Aged; D000975:Antioxidants / Q000008:administration & dosage; D015415:Biomarkers / Q000652:urine*; D000073865:Cigarette Smoking / Q000009:adverse effects; D019587:Dietary Supplements; D004797:Enzyme-Linked Immunosorbent Assay; D028441:F2-Isoprostanes / Q000652:urine*; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects; D012643:Selenium / Q000009:adverse effects*; D014810:Vitamin E / Q000008:administration & dosage*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D028441:F2-Isoprostanes; D014810:Vitamin E; D012643:Selenium
keywords: Alpha-tocopherol; Antioxidant nutrients; Chronic obstructive pulmonary disease; Cigarette smoking; Isoprostanes; Lipid peroxidation; Lung function; Oxidative stress; Randomized controlled trial; Selenium; Vitamin E