title: Single Low-Density Lipoprotein Apheresis Does Not Improve Vascular Endothelial Function in Chronically Treated Hypercholesterolemic Patients.
abstract: Objective. To investigate vascular endothelial function (VEF) responses to a single low-density lipoprotein (LDL) apheresis session in hypercholesterolemic patients undergoing chronic treatment. Methods. We measured brachial artery flow-mediated dilation (FMD), plasma lipids, vitamin E (α- and γ-tocopherol), markers of oxidative/nitrative stress (malondialdehyde (MDA) and nitro-γ-tocopherol (NGT)), and regulators of NO metabolism (arginine (ARG) and asymmetric dimethylarginine (ADMA)) prior to (Pre) and immediately following (Post) LDL apheresis and at 1, 3, 7, and 14 d Post in 5 hypercholesterolemic patients (52 ± 11 y). Results. Relative to Pre, total cholesterol (7.8 ± 1.5 mmol/L) and LDL-cholesterol (6.2 ± 1.2 mmol/L) were 61% and 70% lower (P < 0.01), respectively, at Post and returned to Pre levels at 14 d. Brachial FMD responses (6.9 ± 3.6%) and plasma MDA, ARG, and ADMA concentrations were unaffected by LDL apheresis. Plasma α-tocopherol, γ-tocopherol, and NGT concentrations were 52-69% lower at Post (P < 0.01), and α-tocopherol remained 36% lower at 1 d whereas NGT remained 41% lower at d 3. Conclusions. Acute cholesterol reduction by LDL apheresis does not alter VEF, oxidative stress, or NO homeostasis in patients treated chronically for hypercholesterolemia.
authors.affiliation: Department of Kinesiology and Health, Miami University, Oxford, OH 45056, USA; Division of Cardiology, Hartford Hospital, Hartford, CT 06102, USA.
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Ballard
authors.affiliation: Human Nutrition Program, Department of Human Sciences, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Eunice
authors.initials: E
authors.lastname: Mah
authors.affiliation: Human Nutrition Program, Department of Human Sciences, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Human Nutrition Program, Department of Human Sciences, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Richard S
authors.initials: RS
authors.lastname: Bruno
authors.affiliation: Division of Cardiology, Hartford Hospital, Hartford, CT 06102, USA; Department of Kinesiology, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: Beth A
authors.initials: BA
authors.lastname: Taylor
authors.affiliation: Division of Cardiology, Hartford Hospital, Hartford, CT 06102, USA.
authors.forename: Jo Ellen
authors.initials: JE
authors.lastname: Beam
authors.affiliation: Division of Cardiology, Brigham and Women's Hospital, Boston, MA 02115, USA.
authors.forename: Donna M
authors.initials: DM
authors.lastname: Polk
authors.affiliation: Division of Cardiology, Hartford Hospital, Hartford, CT 06102, USA.
authors.forename: Paul D
authors.initials: PD
authors.lastname: Thompson