title: Protection of DNA during oxidative stress and cytotoxic potential of Artemisia absinthium.
abstract: Medicinal plants are rich in secondary metabolites (alkoloids, glycosides, coumarins, flavonides, steroids, etc.) and considered to be more effective and a safer alternative source to manage a variety of diseases related to liver, heart and kidney disordered. This study determines in vitro antioxidant and in vivo toxicological profile including hemolytic, brine shrimp lethality and mutagenicity of aerial parts of Artemisia absinthium. DNA protection assay was performed on pUC19 plasmid vector using H(2)O(2) as oxidative agent. Total phenolic and flavonoid content were determined using colorimetric methods. Toxicity of the plant was evaluated by brine shrimp lethality, hemolytic and mutagenic activity. DNA protection assay of the plant showed concentration dependent protective effect and at concentration 10μL/mL revealed complete protective effect against H(2)O(2) induced DNA damage. Highest phenolic and flavonoid content was found to be 167.3 (mg GAE 100g DW(-1)) and 14 (mg CE 100g DW(-1)) respectively. Results showed that A. absinthium is potent against standard toxicological procedures, that indicates the presence of bioactive components in the plant and possess antioxidant activity that protects DNA against H(2)O(2) induced oxidative damage. Thus the results showed/support that A. absinthium provides significant health benefits.
authors.affiliation: College of Allied Health Professionals, Directorate of Medical Sciences, Government College University Faisalabad, Pakistan / Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan.
authors.forename: Abid
authors.initials: A
authors.lastname: Ali
authors.affiliation: Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan.
authors.forename: Khalilur
authors.initials: K
authors.lastname: Rahman
authors.affiliation: Department of Chemistry, University of Agriculture, Faisalabad, Pakistan.
authors.forename: Nazish
authors.initials: N
authors.lastname: Jahan
authors.affiliation: Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan.
authors.forename: Amer
authors.initials: A
authors.lastname: Jamil
authors.affiliation: Directorate of Medical Sciences, Government College University Faisalabad, Pakistan.
authors.forename: Abid
authors.initials: A
authors.lastname: Rashid
authors.affiliation: Department of Eastern Medicine, Directorate of Medical Sciences, Government College University Faisalabad, Pakistan.
authors.forename: Syed Muhammad Ali
authors.initials: SM
authors.lastname: Shah
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001156:Artemia; D031171:Artemisia absinthium / Q000737:chemistry*; D001713:Biphenyl Compounds; D003124:Colorimetry; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D004354:Drug Screening Assays, Antitumor; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D009152:Mutagenicity Tests; D018384:Oxidative Stress; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D010851:Picrates; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D010957:Plasmids / Q000235:genetics
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D004247:DNA; C004931:1,1-diphenyl-2-picrylhydrazyl