title: Isolation, Purification and Quantification of Ginsenoside F₅ and F₃ Isomeric Compounds from Crude Extracts of Flower Buds of Panax ginseng.
abstract: In this paper, the isolation, purification and quantification of ginsenoside F₅ and F₃ isomeric compounds from crude extracts of flower buds of Panax ginseng (CEFBPG) was investigated by reversed-phase high-performance liquid chromatography (RP-HPLC) for the first time. The satisfied separation at analytical scale was achieved using a Zorbax Eclipse XDB C-18 column with a ternary mobile phase of acetonitrile-water-phosphoric acid (28:71:1) at a flow rate of 1.0 mL/min within 40 min. UV detection was set at 203 nm. Ginsenoside F₅ and F₃ was 4.21 mg and 5.13 mg in 1 g flower buds of P. ginseng (FBPG), respectively. The preparation of ginsenoside F₅ and F₃ at semi-preparative scale was performed by using a Daisogel C-18 column and gradient elution system of acetonitrile-water (32:68 → 28:72) at a flow rate of 10 mL/min with a sample load of 20-30 mg, and yielded ginsenosides in purity of more than 96%. Their structures were characterized by NMR and high resolution electrospray ionization mass spectrometry (HRESIMS). All the method validations showed acceptable limits. The results indicate a new source to obtain ginsenoside F₅ and F₃, and show that the method developed here appears to be reliable for simultaneously preparing them from CEFBPG.
authors.affiliation: College of Medical, Dalian University, Dalian 116622, China. like905219@163.com.
authors.forename: Ke-Ke
authors.initials: KK
authors.lastname: Li
authors.affiliation: College of Medical, Dalian University, Dalian 116622, China. xufei910324@163.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Xu
authors.affiliation: College of Medical, Dalian University, Dalian 116622, China. gxjclr@163.com.
authors.forename: Xiao-Jie
authors.initials: XJ
authors.lastname: Gong
mesh_terms: D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D045424:Complex Mixtures / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry*; D036145:Ginsenosides / Q000737:chemistry* / Q000302:isolation & purification; D007536:Isomerism; D009682:Magnetic Resonance Spectroscopy; D005894:Panax / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D014867:Water / Q000737:chemistry
chemical_list: D045424:Complex Mixtures; D036145:Ginsenosides; D014867:Water
keywords: ginsenoside F3; ginsenoside F5; isomer; purification; quantification