title: Characterization of a novel double-stranded RNA mycovirus conferring hypovirulence from the phytopathogenic fungus Botryosphaeria dothidea.
abstract: A novel double-stranded RNA (dsRNA) virus, designated as Botryosphaeria dothidea RNA virus 1 (BdRV1), isolated from a hypovirulent strain YZN115 of Botryosphaeria dothidea was biologically and molecularly characterized. The genome of BdRV1 comprises of five dsRNAs. Each dsRNA contains a single open reading frame. The proteins encoded by dsRNA1-4 shared significant amino acid identities of 55%, 47%, 43% and 53% with the corresponding proteins of Aspergillus fumigatus tetramycovirus-1. DsRNA1, 3, and 4 of BdRV1 encoded an RNA-dependent RNA polymerase, a viral methyltransferase, and a P-A-S-rich protein, respectively. Function of proteins encoded by the dsRNA2 and dsRNA5 were unknown. BdRV1 conferred hypovirulence for its host and could be transmitted through conidia and hyphae contact.
authors.affiliation: National Key Laboratory of Agromicrobiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Zhai
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xiang
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Meixin
authors.initials: M
authors.lastname: Zhang
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Min
authors.initials: M
authors.lastname: Fu
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Zuokun
authors.initials: Z
authors.lastname: Yang
authors.affiliation: National Key Laboratory of Agromicrobiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Ni
authors.initials: N
authors.lastname: Hong
authors.affiliation: National Key Laboratory of Agromicrobiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: gpwang@mail.hzau.edu.cn.
authors.forename: Guoping
authors.initials: G
authors.lastname: Wang
mesh_terms: D001203:Ascomycota / Q000145:classification / Q000302:isolation & purification / Q000472:pathogenicity / Q000821:virology*; D061046:Biological Control Agents; D000067148:Fungal Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016679:Genome, Viral; D010449:Peptide Mapping; D010802:Phylogeny; D012328:RNA Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins / Q000302:isolation & purification; D014774:Virulence
chemical_list: D061046:Biological Control Agents; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins
keywords: Botryosphaeria dothidea; Double-stranded RNA virus; Genome; Hypovirulence