title: Phylogenetic analysis of the glycoprotein gene of viral hemorrhagic septicemia virus from Iranian trout farms points towards a common European origin.
abstract: Viral haemorrhagic septicaemia virus (VHSV), a member of family Rhabdoviridae and genus Novirhabdoviridae, causes mortality in numerous marine and freshwater hosts located in northern hemisphere. To evaluate the genetic diversity of VHSV from the North and South West of Iran, the sequences of a 1483bp nt region of the glycoprotein gene were determined for four Iranian isolates. These sequences were analysed to evaluate their genetic relatedness with 86 worldwide isolates representing the four known genogroups of VHSV. Phylogenetic analysis by nucleotide sequences showed that all the VHSV isolates studied were closest related to the 19 fresh water strains from Germany grouped within the European genogroup Ia-2. This finding indicates that Iranian VHSV most likely was introduced to Iran by the movement of contaminated fish fry from a source in Europe.
authors.affiliation: Department of Microbiology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran.
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Ghorani
authors.affiliation: Department of Aquatic Animal Health and Diseases, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran.
authors.forename: Milad
authors.initials: M
authors.lastname: Adel
authors.affiliation: Center of Biotechnology and Biology Research, Shahid Chamran University, Ahvaz, Iran. Electronic address: dadar.m77@gmail.com.
authors.forename: Maryam
authors.initials: M
authors.lastname: Dadar
authors.affiliation: Department of Microbiology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran.
authors.forename: Arash Ghalyanchi
authors.initials: AG
authors.lastname: Langeroudi
authors.affiliation: Iranian Veterinary Organization, Food Laboratory, Tehran, Iran.
authors.forename: Roholla
authors.initials: R
authors.lastname: Kamyabi
authors.affiliation: Institute of Marine and Environmental Technology, University of Maryland Baltimore County, Baltimore 21202, USA.
authors.forename: Vikram N
authors.initials: VN
authors.lastname: Vakharia
authors.affiliation: Qiagen-Aarhus, Aarhus, Denmark.
authors.forename: Katja
authors.initials: K
authors.lastname: Einer-Jensen
mesh_terms: D000818:Animals; D005060:Europe / Q000453:epidemiology; D006023:Glycoproteins / Q000235:genetics*; D031941:Hemorrhagic Septicemia, Viral / Q000453:epidemiology / Q000401:mortality / Q000821:virology*; D007492:Iran / Q000453:epidemiology; D029141:Novirhabdovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017686:Oncorhynchus mykiss / Q000821:virology; D010802:Phylogeny
chemical_list: D006023:Glycoproteins
keywords: Genetic relatedness; Glycoprotein gene; Iran; Rainbow trout; Trading practice