title: Dendritic Cells Are Involved in the Effects of Exercise in a Model of Asthma.
abstract: This study investigated the effects of aerobic exercise (AE) on both the maturation of dendritic cells (DC) and the activation of lymphocytes in a mouse model of chronic allergic airway inflammation.
  
  
  
  C57BL/6 mice distributed into control, exercise, ovalbumin (OVA), and OVA + exercise groups were submitted to OVA sensitization and challenge. Treadmill training was performed for 4 wk, and mice were assessed for classical features of chronic allergic airway inflammation as well as dendritic cell activation and T-lymphocyte response.
  
  
  
  AE reduced OVA-induced eosinophilic inflammation as observed in bronchoalveolar lavage fluid (P < 0.001), airway walls (P < 0001), and also reduced collagen deposition (P < 0.001). AE also reduced bronchoalveolar lavage fluid cytokines (interleukin [IL]-4, P < 0.001; IL-5, P < 0.01; IL-6, P < 0.001; IL-13, P < 0.01; and tumor necrosis factor α, P < 0.01). Cells derived from mediastinal lymphnodes of AE animals that were restimulated with OVA produced less IL-4 (P < 0.01), IL-5 (P < 0.01), and IL-13 (P < 0.001). In addition, AE reduced both DC activation, as demonstrated by reduced release of IL-6 (P < 0.001), CXCL1/KC (P < 0.01), IL-12p70 (P < 0.01), and tumor necrosis factor α (P < 0.05) and DC maturation, as demonstrated by lower MCH-II expression (P < 0.001).
  
  
  
  AE attenuated dendritic cell and lymphocyte activation and maturation, which contributed to reduced airway inflammation and remodeling in the OVA model of chronic allergic airway inflammation.
authors.affiliation: 1Laboratory of Pulmonary and Exercise Immunology (LABPEI), Nove de Julho University (UNINOVE), Sao Paulo, BRAZIL; 2COPD and Asthma Research Group, University Hospital Freigurg, Freiburg, GERMANY; and 3Department of Clinical Medicine (LIM 20), School of Medicine, University of Sao Paulo (USP), Sao Paulo, BRAZIL.
authors.forename: Breanne
authors.initials: B
authors.lastname: Mackenzie
authors.forename: Adilson Santos
authors.initials: AS
authors.lastname: Andrade-Sousa
authors.forename: Manoel Carneiro
authors.initials: MC
authors.lastname: Oliveira-Junior
authors.forename: Erasmo
authors.initials: E
authors.lastname: Assumpção-Neto
authors.forename: Maysa Alves Rodrigues
authors.initials: MA
authors.lastname: Brandão-Rangel
authors.forename: Adriano
authors.initials: A
authors.lastname: Silva-Renno
authors.forename: Alana
authors.initials: A
authors.lastname: Santos-Dias
authors.forename: Sanja
authors.initials: S
authors.lastname: Cicko
authors.forename: Melanie
authors.initials: M
authors.lastname: Grimm
authors.forename: Tobias
authors.initials: T
authors.lastname: Müller
authors.forename: Ana Paula Ligeiro
authors.initials: AP
authors.lastname: Oliveira
authors.forename: Milton Arruda
authors.initials: MA
authors.lastname: Martins
authors.forename: Marco
authors.initials: M
authors.lastname: Idzko
authors.forename: Rodolfo Paula
authors.initials: RP
authors.lastname: Vieira
mesh_terms: D056151:Airway Remodeling / Q000276:immunology; D000818:Animals; D001249:Asthma / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology*; D004195:Disease Models, Animal; D007249:Inflammation / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D008810:Mice, Inbred C57BL; D010805:Physical Conditioning, Animal; D013601:T-Lymphocytes / Q000166:cytology
chemical_list: D016207:Cytokines