title: Evaluation of the mirn23a Cluster through an iTRAQ-based Quantitative Proteomic Approach.
abstract: MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression that are implicated in a number of disease states. MiRNAs can exist as individual entities or may be clustered and transcribed as a single polycistron. The mirn23a cluster consists of three miRNAs: miR-23a, miR-24-2, and miR-27a. Although these miRNAs are transcribed together, they often exist at varying levels in the cell. Despite the fact that the mirn23a cluster is known to play a role in a number of diseases and developmental processes, few direct targets have been identified. In this study, we examined the effects of miR-23a, miR-24-2, miR-27a, or the mirn23a cluster overexpression on the proteome of 70Z/3 pre-B lymphoblast cells. Quantitative mass spectrometry using isobaric tags for relative and absolute quantification (iTRAQ) allowed for the global profiling of cell lines after miRNA overexpression. We identified a number of targets of each miRNA that contained predicted miRNA seed sequences and are likely direct targets. In addition, we discovered a cohort of shared miRNA targets and cluster targets, demonstrating the importance of studying miRNA clusters in their entirety.
authors.affiliation: Harper Cancer Research Institute, University of Notre Dame , Notre Dame, Indiana 46617 United States.
authors.forename: Katelyn R
authors.initials: KR
authors.lastname: Ludwig
authors.affiliation: Harper Cancer Research Institute, University of Notre Dame , Notre Dame, Indiana 46617 United States.|Department of Microbiology and Immunology, Indiana University School of Medicine , South Bend, Indiana 46202 United States.
authors.forename: Richard
authors.initials: R
authors.lastname: Dahl
authors.affiliation: Harper Cancer Research Institute, University of Notre Dame , Notre Dame, Indiana 46617 United States.
authors.forename: Amanda B
authors.initials: AB
authors.lastname: Hummon
mesh_terms: D002460:Cell Line; D005786:Gene Expression Regulation / Q000235:genetics; D006801:Humans; D008213:Lymphocyte Activation / Q000235:genetics; D035683:MicroRNAs / Q000032:analysis* / Q000502:physiology; D005810:Multigene Family / Q000502:physiology*; D054448:Precursor Cells, B-Lymphoid / Q000737:chemistry / Q000166:cytology; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
chemical_list: C552430:MIRN23a microRNA, human; C543845:MIRN24 microRNA, human; C531388:MIRN27 microRNA, human; D035683:MicroRNAs; D020543:Proteome
keywords: cluster; iTRAQ; miR-23a; miR-24; miR-27a; microRNA