title: Depression of Macrophages Modifies Serum Lipid Profile in Hyperlipidemia.
abstract: Suppression of functional activity of macrophages by gadolinium chloride, suppressing the macrophage population and the endocytosis velocity, was studied in vivo. Injection of GdCl3 led to an increase in serum cholesterol concentration. Preliminary injection of GdCl3 to mice with lipidemia 24 h before poloxamer 407 reduced the concentrations of triglycerides and LDL during marked depression of macrophages (in 24 h). Macrophage repopulation (days 5, 7) was associated with development of a trend to an increase of triglyceride and LDL levels. lectron microscopic study of Kupffer cells after injection of poloxamer 407 and its combination with gadoliniun chloride detected the intralysosomal accumulation syndrome in these cells (formation of auto- and heterophagolysosomes). Activity of cathepsin B, characteristic of macrophages, reduced 24 h after injections of GdCl3 and poloxamer 407 alone and restored in response to their combination.
authors.affiliation: Institute of Physiology and Basic Medicine, Novosibirsk, Russia. pathol@inbox.ru.
authors.forename: N V
authors.initials: NV
authors.lastname: Goncharova
authors.affiliation: Novosibirsk State Medical University, Ministry of Health of the Russian Federation, Novosibirsk, Russia.
authors.forename: A B
authors.initials: AB
authors.lastname: Pupyshev
authors.affiliation: Institute of Physiology and Basic Medicine, Novosibirsk, Russia.
authors.forename: E E
authors.initials: EE
authors.lastname: Filyushina
authors.affiliation: Institute of Physiology and Basic Medicine, Novosibirsk, Russia.
authors.forename: K V
authors.initials: KV
authors.lastname: Loktev
authors.affiliation: University Canada West, Vancouver, Canada.
authors.forename: E Ts
authors.initials: ETs
authors.lastname: Korolenko
authors.affiliation: Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia.
authors.forename: E L
authors.initials: EL
authors.lastname: Lushnikova
authors.affiliation: Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia.
authors.forename: O P
authors.initials: OP
authors.lastname: Molodykh
authors.affiliation: Institute of Physiology and Basic Medicine, Novosibirsk, Russia.
authors.forename: T A
authors.initials: TA
authors.lastname: Korolenko
authors.affiliation: Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia.
authors.forename: B V
authors.initials: BV
authors.lastname: Churin
mesh_terms: D000818:Animals; D002401:Cathepsin B / Q000378:metabolism*; D049109:Cell Proliferation; D002784:Cholesterol / Q000097:blood; D004705:Endocytosis / Q000276:immunology; D005682:Gadolinium / Q000494:pharmacology*; D006949:Hyperlipidemias / Q000097:blood* / Q000276:immunology; D007728:Kupffer Cells / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000097:blood; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D020442:Poloxamer / Q000494:pharmacology*; D014280:Triglycerides / Q000097:blood
chemical_list: D008077:Lipoproteins, LDL; D014280:Triglycerides; D020442:Poloxamer; D002784:Cholesterol; D005682:Gadolinium; D002401:Cathepsin B; C533915:Ctsb protein, mouse; C038958:gadolinium chloride
keywords: gadolinium chloride; hyperlipidemia; intralysosomal accumulation; macrophage depression