title: STEROL PROFILES IN 18 MACROALGAE OF THE PORTUGUESE COAST(1).
abstract: The sterol profiles of dominant macroalgae occurring in the western Portuguese coast were evaluated. An analytical procedure, involving alkaline hydrolysis and extraction followed by separation by reversed-phase HPLC-diode array detection (HPLC-DAD), was optimized for the study of their sterols composition. The validated methodology is short in analysis time (as the compounds are determined in <20 min), sensitive, reproducible, and accurate. It was then successfully applied to the determination of campesterol, cholesterol, desmosterol, ergosterol, fucosterol, stigmasterol, and β-sitosterol in 18 species (three Chlorophyta, five Rhodophyta, and 10 Phaeophyta). The profiles obtained for the several macroalgal species were considerably different. C29 sterols were predominant in Phaeophyta and Chlorophyta (71%-95% of total sterol content), while in Rhodophyta cholesterol content is significantly higher (34%-87%). Among the studied species, Asparagopsis armata Harv. contained the lowest sterol amount (555 mg · kg(-1) dry weight), and Cystoseira tamariscifolia (Huds.) Papenf. the highest one (6,502 mg · kg(-1) dry weight). Data obtained may be helpful in identifying suitable marine sources of sterols, with potential applications in the food and pharmaceutical industries.
authors.affiliation: REQUIMTE/Laboratório de Farmacognosia, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha 164, 4050-047 Porto, PortugalResearch Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100 Campus University Espinardo, Murcia, SpainGIRM - Marine Resources Research Group, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, Santuário N.ª Sra. Dos Remédios, Apartado 126, 2524-909 Peniche, Portugal.
authors.forename: Graciliana
authors.initials: G
authors.lastname: Lopes
authors.affiliation: REQUIMTE/Laboratório de Farmacognosia, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha 164, 4050-047 Porto, PortugalResearch Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100 Campus University Espinardo, Murcia, SpainGIRM - Marine Resources Research Group, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, Santuário N.ª Sra. Dos Remédios, Apartado 126, 2524-909 Peniche, Portugal.
authors.forename: Carla
authors.initials: C
authors.lastname: Sousa
authors.affiliation: REQUIMTE/Laboratório de Farmacognosia, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha 164, 4050-047 Porto, PortugalResearch Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100 Campus University Espinardo, Murcia, SpainGIRM - Marine Resources Research Group, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, Santuário N.ª Sra. Dos Remédios, Apartado 126, 2524-909 Peniche, Portugal.
authors.forename: João
authors.initials: J
authors.lastname: Bernardo
authors.affiliation: REQUIMTE/Laboratório de Farmacognosia, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha 164, 4050-047 Porto, PortugalResearch Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100 Campus University Espinardo, Murcia, SpainGIRM - Marine Resources Research Group, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, Santuário N.ª Sra. Dos Remédios, Apartado 126, 2524-909 Peniche, Portugal.
authors.forename: Paula B
authors.initials: PB
authors.lastname: Andrade
authors.affiliation: REQUIMTE/Laboratório de Farmacognosia, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha 164, 4050-047 Porto, PortugalResearch Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100 Campus University Espinardo, Murcia, SpainGIRM - Marine Resources Research Group, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, Santuário N.ª Sra. Dos Remédios, Apartado 126, 2524-909 Peniche, Portugal.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Valentão
authors.affiliation: REQUIMTE/Laboratório de Farmacognosia, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha 164, 4050-047 Porto, PortugalResearch Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100 Campus University Espinardo, Murcia, SpainGIRM - Marine Resources Research Group, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, Santuário N.ª Sra. Dos Remédios, Apartado 126, 2524-909 Peniche, Portugal.
authors.forename: Federico
authors.initials: F
authors.lastname: Ferreres
authors.affiliation: REQUIMTE/Laboratório de Farmacognosia, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha 164, 4050-047 Porto, PortugalResearch Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100 Campus University Espinardo, Murcia, SpainGIRM - Marine Resources Research Group, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, Santuário N.ª Sra. Dos Remédios, Apartado 126, 2524-909 Peniche, Portugal.
authors.forename: Teresa
authors.initials: T
authors.lastname: Mouga
keywords: Chlorophyta; Phaeophyta; Rhodophyta; macroalgae; sterols