title: Dielectric study of interaction of water with normal and osteoarthritis femoral condyle cartilage.
abstract: The main goal of this paper is the in vitro study of healthy and osteoarthritis (OA) human cartilage using the dielectric spectroscopy in the alpha-dispersion region of the electric field and in the temperatures from 25 to 140°C. The activation energy of conductivity needed to break the bonds formed by water in the extracellular matrix takes the average values of 61kJ/mol and 44kJ/mol for the control and OA cartilages, respectively. At 28°C, the small difference appears in the permittivity decrement between the control and OA cartilages, while the conductivity increment is about 2 times higher for the control tissue than that for the OA tissue. At 75°C, the conductivity increment for both of these samples is 8 times higher than their respective permittivity decrement. In addition, at 140°C the values of these both parameters for the OA tissue decrease by 8 times as compared to those recorded for the control sample. The relaxation frequency of about 10kHz is similar for both of these samples. The knowledge on dielectric properties of healthy and OA cartilage may prove relevant to tissue engineering focused on the repair of cartilage lesions via the layered structure designing.
authors.affiliation: Department of Bionics and Bioimpedance, University of Medical Sciences, Parkowa 2, 60-775 Poznań, Poland. Electronic address: ewaklcde@amu.edu.pl.
authors.forename: E
authors.initials: E
authors.lastname: Marzec
authors.affiliation: Department of Bionics and Bioimpedance, University of Medical Sciences, Parkowa 2, 60-775 Poznań, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Olszewski
authors.affiliation: Department of Orthopedics and Traumatology, University of Medical Sciences, 28 Czerwca 1956r., 135/147, 60-545 Poznań, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Kaczmarczyk
authors.affiliation: Department of Orthopedics and Traumatology, University of Medical Sciences, 28 Czerwca 1956r., 135/147, 60-545 Poznań, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Richter
authors.affiliation: Department of Orthopedics and Traumatology, University of Medical Sciences, 28 Czerwca 1956r., 135/147, 60-545 Poznań, Poland.
authors.forename: T
authors.initials: T
authors.lastname: Trzeciak
authors.affiliation: Department of Orthopedics and Traumatology, University of Medical Sciences, 28 Czerwca 1956r., 135/147, 60-545 Poznań, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Nowocień
authors.affiliation: Department of Rheumatology and Rehabilitation, University of Medical Sciences, 28 Czerwca 1956r., 135/147, 60-545 Poznań, Poland.
authors.forename: R
authors.initials: R
authors.lastname: Malak
authors.affiliation: Department of Rheumatology and Rehabilitation, University of Medical Sciences, 28 Czerwca 1956r., 135/147, 60-545 Poznań, Poland.
authors.forename: W
authors.initials: W
authors.lastname: Samborski
mesh_terms: D000328:Adult; D002358:Cartilage, Articular / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism; D058266:Dielectric Spectroscopy; D005269:Femur / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D010003:Osteoarthritis / Q000378:metabolism*; D013696:Temperature; D014867:Water / Q000378:metabolism*
chemical_list: D006025:Glycosaminoglycans; D014867:Water; D003094:Collagen
keywords: Dielectric spectroscopy; Electrical conduction; Osteoarthritis cartilage; Temperature; Water protons