title: Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes.
abstract: A cell-free expression platform for making bacterial ribosomes encapsulated within giant liposomes was capable of synthesizing sfGFP. The liposomes were prepared using a double emulsion template, and compartmentalized in vitro protein synthesis was analysed using spinning disk confocal microscopy. Two different liposome phospholipid formulations were investigated to characterize their effects on the compartmentalized reaction kinetics. This study was performed as a necessary step towards the synthesis of minimal cells.
authors.affiliation: Department of Chemical and Biochemical Engineering, Chemistry of Life Processes Institute, Simpson Querrey Institute for BioNanotechnology, Northwestern University, Evanston, 60208, Illinois, USA. m-jewett@northwestern.edu.
authors.forename: Filippo
authors.initials: F
authors.lastname: Caschera
authors.affiliation: Department of Mechanical Engineering, University of Michigan, Ann Arbor, 48109, Michigan, USA. allenliu@unimich.edu.
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Lee
authors.affiliation: Department of Mechanical Engineering, University of Michigan, Ann Arbor, 48109, Michigan, USA. allenliu@unimich.edu.
authors.forename: Kenneth K Y
authors.initials: KK
authors.lastname: Ho
authors.affiliation: Department of Mechanical Engineering, University of Michigan, Ann Arbor, 48109, Michigan, USA. allenliu@unimich.edu.
authors.forename: Allen P
authors.initials: AP
authors.lastname: Liu
authors.affiliation: Department of Chemical and Biochemical Engineering, Chemistry of Life Processes Institute, Simpson Querrey Institute for BioNanotechnology, Northwestern University, Evanston, 60208, Illinois, USA. m-jewett@northwestern.edu.
authors.forename: Michael C
authors.initials: MC
authors.lastname: Jewett
mesh_terms: D002474:Cell-Free System / Q000737:chemistry* / Q000378:metabolism; D004655:Emulsions / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000235:genetics; D008081:Liposomes / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry; D014176:Protein Biosynthesis; D012270:Ribosomes / Q000737:chemistry* / Q000235:genetics
chemical_list: D004655:Emulsions; D008081:Liposomes; D010743:Phospholipids; D049452:Green Fluorescent Proteins