title: A new method using insert-based systems (IBS) to improve cell behavior study on flexible and rigid biomaterials.
abstract: In vitro studies about biomaterials biological properties are essential screening tests. Yet cell cultures encounter difficulties related to cell retention on material surface or to the observation of both faces of permeable materials. The objective of the present study was to develop a reliable in vitro method to study cell behavior on rigid and flexible/permeable biomaterials elaborating two specific insert-based systems (IBS-R and IBS-F respectively). IBS-R was designed as a specific cylindrical polytetrafluoroethylene (PTFE) system to evaluate attachment, proliferation and morphology of human gingival fibroblasts (HGFs) on grade V titanium and lithium disilicate glass-ceramic discs characteristics of dental prostheses. The number of cells, their covering on discs and their morphology were determined from MTS assays and microscopic fluorescent images after 24, 48 and 72 h. IBS-F was developed as a two components system to study HGFs behavior on guided bone regeneration polyester membranes. The viability and the membrane barrier effect were evaluated by metabolic MTS assays and by scanning electron microscopy. IBS-R and IBS-F were shown to promote (1) easy and rapid handling; (2) cell retention on biomaterial surface; (3) accurate evaluation of the cellular proliferation, spreading and viability; (4) use of non-toxic material. Moreover IBS-F allowed the study of the cell migration through degradable membranes, with an access to both faces of the biomaterial and to the bottom of culture wells for medium changing.
authors.affiliation: Dental Biomaterials Research Unit (d-BRU) and Department of Fixed Prosthodontics, Institute of Dentistry, University of Liège (ULg) and University of Liège Hospital (CHU), Quai Godefroid Kurth 45, 4020, Liège, Belgium. charlotte.grenade@chu.ulg.ac.be.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Grenade
authors.affiliation: Mammalian Cell Culture Laboratory, GIGA-R, University of Liège (ULg), Liège, Belgium.|GlaxoSmithKline Vaccines, Parc de la Noire Epine, Rue Fleming, 20, 1300-, Wavre, Belgium.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Moniotte
authors.affiliation: Department of Periodontology and Oral Surgery, Dental Biomaterials Research Unit (d-BRU), Institute of Dentistry, University of Liège (ULg) and University of Liège Hospital (CHU), Liège, Belgium.
authors.forename: Eric
authors.initials: E
authors.lastname: Rompen
authors.affiliation: Dental Biomaterials Research Unit (d-BRU) and Department of Fixed Prosthodontics, Institute of Dentistry, University of Liège (ULg) and University of Liège Hospital (CHU), Quai Godefroid Kurth 45, 4020, Liège, Belgium.
authors.forename: Alain
authors.initials: A
authors.lastname: Vanheusden
authors.affiliation: Dental Biomaterials Research Unit (d-BRU) and Department of Fixed Prosthodontics, Institute of Dentistry, University of Liège (ULg) and University of Liège Hospital (CHU), Quai Godefroid Kurth 45, 4020, Liège, Belgium.
authors.forename: Amélie
authors.initials: A
authors.lastname: Mainjot
authors.affiliation: Mammalian Cell Culture Laboratory, GIGA-R, University of Liège (ULg), Liège, Belgium.
authors.forename: Marie-Claire
authors.initials: MC
authors.lastname: De Pauw-Gillet
keywords: Biomaterials; Cytocompatibility; Dental prostheses; Human gingival fibroblasts; Insert; Membranes