title: Kinetic analysis reveals the diversity of microscopic mechanisms through which molecular chaperones suppress amyloid formation.
abstract: It is increasingly recognized that molecular chaperones play a key role in modulating the formation of amyloid fibrils, a process associated with a wide range of human disorders. Understanding the detailed mechanisms by which they perform this function, however, has been challenging because of the great complexity of the protein aggregation process itself. In this work, we build on a previous kinetic approach and develop a model that considers pairwise interactions between molecular chaperones and different protein species to identify the protein components targeted by the chaperones and the corresponding microscopic reaction steps that are inhibited. We show that these interactions conserve the topology of the unperturbed reaction network but modify the connectivity weights between the different microscopic steps. Moreover, by analysing several protein-molecular chaperone systems, we reveal the striking diversity in the microscopic mechanisms by which molecular chaperones act to suppress amyloid formation.
authors.affiliation: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.
authors.forename: Paolo
authors.initials: P
authors.lastname: Arosio
authors.affiliation: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.
authors.forename: Thomas C T
authors.initials: TC
authors.lastname: Michaels
authors.affiliation: Department of Biochemistry and Structural Biology, Lund University, Box 124, SE221 00 Lund, Sweden.
authors.forename: Sara
authors.initials: S
authors.lastname: Linse
authors.affiliation: Department of Biochemistry and Structural Biology, Lund University, Box 124, SE221 00 Lund, Sweden.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Månsson
authors.affiliation: Department of Biochemistry and Structural Biology, Lund University, Box 124, SE221 00 Lund, Sweden.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Emanuelsson
authors.affiliation: Center for Alzheimer Research, Division for Neurogeriatrics, Department of Neurobiology, Care Sciences and Society, Karolinska Institutet, S-141 57 Huddinge, Sweden.
authors.forename: Jenny
authors.initials: J
authors.lastname: Presto
authors.affiliation: Center for Alzheimer Research, Division for Neurogeriatrics, Department of Neurobiology, Care Sciences and Society, Karolinska Institutet, S-141 57 Huddinge, Sweden.
authors.forename: Jan
authors.initials: J
authors.lastname: Johansson
authors.affiliation: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.
authors.forename: Michele
authors.initials: M
authors.lastname: Vendruscolo
authors.affiliation: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Dobson
authors.affiliation: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.
authors.forename: Tuomas P J
authors.initials: TP
authors.lastname: Knowles
mesh_terms: D000682:Amyloid / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000378:metabolism*
chemical_list: D000682:Amyloid; D016229:Amyloid beta-Peptides; D018832:Molecular Chaperones