title: Different components of conditioned food aversion memory.
abstract: Memory reconsolidation processes and protein kinase Mzeta (PKMzeta) activity in memory maintenance and reorganization are poorly understood. Therefore, we examined memory reconsolidation and PKMzeta activity during the maintenance and reorganization of a conditioned food aversion memory among snails. These processes were specifically evaluated after administration of a serotonin receptor antagonist (methiothepin), NMDA glutamate receptor antagonist (MK-801), protein synthesis inhibitor (cycloheximide; CYH), or PKMzeta inhibitor (zeta inhibitory peptide; ZIP) either 2 or 10 days after aversion training. Two days post-training, injections of MK-801 or CYH, combined with a conditioned stimulus reminder, caused amnesia development, and a second training 11 days after this induction did not lead to long-term memory formation. Interestingly, MK-801 or CYH injections and the reminder 10 days after training did not affect memory retrieval. Methiothepin and the reminder, or ZIP without the reminder, at 2 and 10 days after training led to memory impairment, while a second training 11 days after amnesia induction resulted in memory formation. These results suggest that the maintenance of a conditioned food aversion involves two different components with variable dynamics. One component could be characterized by memory strengthening over time and involve N-methyl-D-aspartate receptors and protein synthesis reconsolidation at early, but not late, training stages. The other memory component could involve serotonin-dependent reconsolidation and Mzeta-like kinase activity at both early and late stages after learning. Deficiencies within these two components led to various forms of memory impairment, which differed in terms of the formation of a conditioned food aversion during the second training.
authors.affiliation: P.K. Anokhin Institute of Normal Physiology, Laboratory of Neurochemistry, Mokhovaya 11/4, 125009 Moscow, Russian Federation. Electronic address: vpnikitin48@gmail.com.
authors.forename: Vladimir P
authors.initials: VP
authors.lastname: Nikitin
authors.affiliation: P.K. Anokhin Institute of Normal Physiology, Laboratory of Neurochemistry, Mokhovaya 11/4, 125009 Moscow, Russian Federation. Electronic address: sols3@yandex.ru.
authors.forename: Svetlana V
authors.initials: SV
authors.lastname: Solntseva
authors.affiliation: P.K. Anokhin Institute of Normal Physiology, Laboratory of Neurochemistry, Mokhovaya 11/4, 125009 Moscow, Russian Federation.
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Kozyrev
authors.affiliation: P.K. Anokhin Institute of Normal Physiology, Laboratory of Neurochemistry, Mokhovaya 11/4, 125009 Moscow, Russian Federation.
authors.forename: Pavel V
authors.initials: PV
authors.lastname: Nikitin
authors.affiliation: P.K. Anokhin Institute of Normal Physiology, Laboratory of Neurochemistry, Mokhovaya 11/4, 125009 Moscow, Russian Federation; Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, 600 North Wolfe Street, CMSC 9-115, Baltimore, MD 21287, United States. Electronic address: shevelkin@gmail.com.
authors.forename: Alexey V
authors.initials: AV
authors.lastname: Shevelkin
mesh_terms: D000818:Animals; D003214:Conditioning, Classical / Q000187:drug effects / Q000502:physiology*; D003513:Cycloheximide / Q000008:administration & dosage; D016291:Dizocilpine Maleate / Q000008:administration & dosage; D018691:Excitatory Amino Acid Antagonists / Q000008:administration & dosage; D005518:Food Preferences / Q000187:drug effects / Q000502:physiology*; D006372:Helix, Snails; D000069077:Memory Consolidation / Q000187:drug effects / Q000502:physiology*; D008719:Methiothepin / Q000008:administration & dosage; D014176:Protein Biosynthesis / Q000187:drug effects; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000502:physiology*; D011500:Protein Synthesis Inhibitors; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors / Q000502:physiology; D011985:Receptors, Serotonin / Q000502:physiology*; D012702:Serotonin Antagonists / Q000008:administration & dosage; D000099221:Protein Kinase C zeta
chemical_list: D003513:Cycloheximide; D016291:Dizocilpine Maleate; D018691:Excitatory Amino Acid Antagonists; D008719:Methiothepin; D011493:Protein Kinase C; D011500:Protein Synthesis Inhibitors; D016194:Receptors, N-Methyl-D-Aspartate; D011985:Receptors, Serotonin; D012702:Serotonin Antagonists; D000099221:Protein Kinase C zeta
keywords: Memory reconsolidation; Memory reorganization; Mzeta protein kinase; Neurotransmitter receptors; Protein synthesis