title: Bystander effects and compartmental stress response to X-ray irradiation in L929 cells.
abstract: Bystander effects are indirect consequences of radiation and many other stress factors. They occur in cells that are not directly exposed to these factors, but receive signals from affected cells either by gap junctions or by molecules released in the medium. Characterizing these effects and deciphering the underlying mechanisms involved in radiation-induced bystander effects are relevant for cancer radiotherapy and radioprotection. At doses of X-ray radiation 0.5 and 1 Gy, we detected bystander effects as increased numbers of micronuclei shortly after the treatment, through medium transfer and by co-cultures. Interestingly, bystander cells did not exhibit long-term adverse changes in viability. Evaluation of several compartmental stress markers (CHOP, BiP, mtHsp60, cytHsp70) by qRT-PCR did not reveal expression changes at transcriptional level. We investigated the involvement of ROS and NO in this process by addition of specific scavengers of these molecules, DMSO or c-PTIO in the transferred medium. This approach proved that ROS but not NO is involved in the induction of lesions in the acceptor cells. These results indicate that L929 cells are susceptible to stress effects of radiation-induced bystander signaling.
authors.affiliation: Horia Hulubei National Institute of Physics and Nuclear Engineering - IFIN-HH, 30 Reactorului St., P.O. BOX MG-6, Magurele, Bucharest, Romania.
authors.forename: Mihaela
authors.initials: M
authors.lastname: Temelie
authors.affiliation: The Oncologic Radiotherapy Clinic, The Coltea Bucharest Hospital, No. 1-3, I. C. Bratianu Boulevard, District 3, Bucharest, Romania.
authors.forename: Daniela
authors.initials: D
authors.lastname: Stroe
authors.affiliation: Horia Hulubei National Institute of Physics and Nuclear Engineering - IFIN-HH, 30 Reactorului St., P.O. BOX MG-6, Magurele, Bucharest, Romania.
authors.forename: Ileana
authors.initials: I
authors.lastname: Petcu
authors.affiliation: Horia Hulubei National Institute of Physics and Nuclear Engineering - IFIN-HH, 30 Reactorului St., P.O. BOX MG-6, Magurele, Bucharest, Romania.
authors.forename: Cosmin
authors.initials: C
authors.lastname: Mustaciosu
authors.affiliation: Neuroscience Psychology and Behaviour Department, University of Leicester, Maurice Shock Building, University Road, Leicester, LE1 9HN, UK.|Faculty of Health and Life Sciences, Leicester School of Pharmacy, The Gateway, De Montfort University, Leicester, LE1 9BH, UK.
authors.forename: Nicoleta
authors.initials: N
authors.lastname: Moisoi
authors.affiliation: Horia Hulubei National Institute of Physics and Nuclear Engineering - IFIN-HH, 30 Reactorului St., P.O. BOX MG-6, Magurele, Bucharest, Romania. dsavu@nipne.ro.
authors.forename: Diana
authors.identifier: 0000-0002-0145-5176
authors.initials: D
authors.lastname: Savu
mesh_terms: D000818:Animals; D024201:Bystander Effect; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D018834:Chaperonin 60; D000091342:Endoplasmic Reticulum Chaperone BiP; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D051379:Mice; D048629:Micronuclei, Chromosome-Defective; D024101:Mitochondrial Proteins; D009569:Nitric Oxide / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013312:Stress, Physiological; D051742:Transcription Factor CHOP; D014965:X-Rays
chemical_list: D018834:Chaperonin 60; C497250:Ddit3 protein, mouse; D000091342:Endoplasmic Reticulum Chaperone BiP; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C557066:Hspd1 protein, mouse; D024101:Mitochondrial Proteins; D017382:Reactive Oxygen Species; D051742:Transcription Factor CHOP; D009569:Nitric Oxide
keywords: Bystander effect; Compartmental stress response; DNA damage; X-rays