title: Optimization of Cultural Conditions for Antioxidant Exopolysaccharides from Xerocomus badius Grown in Shrimp Byproduct.
abstract: To optimize the production conditions for exopolysaccharides with higher antioxidant activities from Xerocomus badius cultured in shrimp byproduct medium, Plackett-Burman design, path of steepest ascent, and response surface methodology were explored. Based on the results of Plackett-Burman design and path of steepest ascent, a Box-Behnken design was applied to optimization and the regression models. The optimal cultural condition for high yield and antioxidant activity of the exopolysaccharides was determined to be 10.347% of solid-to-liquid ratio, a 4.322% content of bran powder, and a 1.217% concentration of glacial acetic acid. Culturing with the optimal cultural conditions resulted in an exopolysaccharides yield of 4.588 ± 0.346 g/L and a total antioxidant activity of 2.956 ± 0.105 U/mg. These values are consistent with the values predicted by the corresponding regression models (RSD < 5%).
authors.affiliation: School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai 264209, China.
authors.forename: Xiujun
authors.initials: X
authors.lastname: Gao
authors.affiliation: School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai 264209, China.
authors.forename: Peisheng
authors.initials: P
authors.lastname: Yan
authors.affiliation: Department of Biological & Agricultural Engineering, North Carolina State University, Raleigh, NC 27695, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai 264209, China.
authors.forename: Jianbing
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai 264209, China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Yu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001487:Basidiomycota / Q000254:growth & development*; D003470:Culture Media / Q000737:chemistry; D062610:Fungal Polysaccharides / Q000096:biosynthesis*; D033561:Penaeidae / Q000737:chemistry
chemical_list: D000975:Antioxidants; D003470:Culture Media; D062610:Fungal Polysaccharides