title: Interaction between STAT3 gene polymorphisms and smoking on Crohn's disease susceptibility: a case-control study in a Chinese Han population.
abstract: OBJECTIVE
  The aim of this study was to investigate the impact of the signal transducer and activator of transcription 3 (STAT3) gene and additional STAT3 gene-smoking interaction on Crohn's disease (CD) risk based on a Chinese population.
  
  
  METHODS
  A total of 1012 participants (491 men, 521 women), were selected, including 502 CD patients and 510 normal controls. The mean age of all participants was 42.3 ± 11.2 years. Logistic regression model was used to examine the association between single nucleotide polymorphism (SNP) of STAT3 and CD risk; the odds ratio (OR) and 95 % confident interval (95 % CI) were calculated. Generalized multifactor dimensionality reduction was employed to analyze the interaction among several SNPs.
  
  
  RESULTS
  Logistic analysis showed the significant association between genotypes of variants in two SNP and decreased CD risk, after covariates adjustment. The carriers of homozygous mutant of two SNP polymorphism revealed decreased CD risk than those with wild-type homozygotes; OR (95 % CI) was 0.75 (0.59-0.93) and 0.68 (0.57-0.91), respectively. There was a significant two-locus model (p = 0.0107) involving rs744166 and smoking, indicating a potential gene-environment interaction between rs744166 and smoking. Overall, the cross-validation consistency was 10/10, and the testing accuracy was 62.17 %, and never smokers with TC or CC genotype have the lowest CD risk, compared to smokers with TT genotype; OR (95 % CI) was 0.52 (0.31-0.82), after covariate adjustment.
  
  
  CONCLUSIONS
  Our results support an important association of rs744166 and rs4796793 with decreased CD risk, and additional interaction between rs744166 and smoking.
authors.affiliation: Inspection Center, Changzhou Center for Disease Control and Prevention, No. 203, Taishan Road, The New North District, Changzhou, 213022, Jiangsu, People's Republic of China.
authors.forename: Jun-Hong
authors.initials: JH
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory, The First Hospital of Hengyang, Hengyang, 421001, Hunan, People's Republic of China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wu
authors.affiliation: Department of Blood Quality Management, Changzhou Blood Center, Changzhou, 213004, Jiangsu, People's Republic of China.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Yang
authors.affiliation: Inspection Center, Changzhou Center for Disease Control and Prevention, No. 203, Taishan Road, The New North District, Changzhou, 213022, Jiangsu, People's Republic of China.
authors.forename: Hong-Bing
authors.initials: HB
authors.lastname: Tang
authors.affiliation: Inspection Center, Changzhou Center for Disease Control and Prevention, No. 203, Taishan Road, The New North District, Changzhou, 213022, Jiangsu, People's Republic of China.
authors.forename: Dong-Ping
authors.initials: DP
authors.lastname: Bao
authors.affiliation: Inspection Center, Changzhou Center for Disease Control and Prevention, No. 203, Taishan Road, The New North District, Changzhou, 213022, Jiangsu, People's Republic of China. jijunmin3313@163.com.
authors.forename: Jun-Min
authors.initials: JM
authors.lastname: Ji
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D003424:Crohn Disease / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D050796:STAT3 Transcription Factor / Q000235:genetics*; D012907:Smoking / Q000453:epidemiology / Q000235:genetics*
chemical_list: D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human
keywords: Crohn’s disease; Interaction; SNP; STAT3; Smoking