title: Acute exercise restores insulin clearance in diet-induced obese mice.
abstract: The aim of this study was to investigate the insulin clearance in diet-induced obese (DIO) mice submitted to acute endurance exercise (3h of treadmill exercise at 60-70% VO2max). Glucose-stimulated insulin secretion in isolated islets; ipGTT; ipITT; ipPTT; in vivo insulin clearance; protein expression in liver, skeletal muscle, and adipose tissue (insulin degrading enzyme (IDE), insulin receptor subunitβ(IRβ), phospho-Akt (p-Akt) and phospho-AMPK (p-AMPK)), and the activity of IDE in the liver and skeletal muscle were accessed. In DIO mice, acute exercise reduced fasting glycemia and insulinemia, improved glucose and insulin tolerance, reduced hepatic glucose production, and increased p-Akt protein levels in liver and skeletal muscle and p-AMPK protein levels in skeletal muscle. In addition, insulin secretion was reduced, whereas insulin clearance and the expression of IDE and IRβ were increased in liver and skeletal muscle. Finally, IDE activity was increased only in skeletal muscle. In conclusion, we propose that the increased insulin clearance and IDE expression and activity, primarily, in skeletal muscle, constitute an additional mechanism, whereby physical exercise reduces insulinemia in DIO mice.
authors.affiliation: Department of Structural and Functional BiologyInstitute of Biology, State University of Campinas (UNICAMP), Campinas, Brazil.
authors.forename: Mirian A
authors.initials: MA
authors.lastname: Kurauti
authors.affiliation: Department of Structural and Functional BiologyInstitute of Biology, State University of Campinas (UNICAMP), Campinas, Brazil.
authors.forename: José M
authors.initials: JM
authors.lastname: Costa-Júnior
authors.affiliation: Department of Structural and Functional BiologyInstitute of Biology, State University of Campinas (UNICAMP), Campinas, Brazil.
authors.forename: Sandra M
authors.initials: SM
authors.lastname: Ferreira
authors.affiliation: Department of Structural and Functional BiologyInstitute of Biology, State University of Campinas (UNICAMP), Campinas, Brazil.
authors.forename: Gustavo J
authors.initials: GJ
authors.lastname: Dos Santos
authors.affiliation: Department of Structural and Functional BiologyInstitute of Biology, State University of Campinas (UNICAMP), Campinas, Brazil.
authors.forename: André O P
authors.initials: AO
authors.lastname: Protzek
authors.affiliation: Department of Structural and Functional BiologyInstitute of Biology, State University of Campinas (UNICAMP), Campinas, Brazil.
authors.forename: Tarlliza R
authors.initials: TR
authors.lastname: Nardelli
authors.affiliation: Department of Structural and Functional BiologyInstitute of Biology, State University of Campinas (UNICAMP), Campinas, Brazil.
authors.forename: Luiz F
authors.initials: LF
authors.lastname: de Rezende
authors.affiliation: Department of Structural and Functional BiologyInstitute of Biology, State University of Campinas (UNICAMP), Campinas, Brazil boschero@unicamp.br.
authors.forename: Antonio C
authors.initials: AC
authors.lastname: Boschero
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D000818:Animals; D002096:C-Peptide / Q000097:blood; D059305:Diet, High-Fat / Q000009:adverse effects; D005947:Glucose / Q000378:metabolism; D007328:Insulin / Q000097:blood / Q000378:metabolism*; D007339:Insulysin / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D008657:Metabolic Clearance Rate; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D018482:Muscle, Skeletal / Q000378:metabolism; D009765:Obesity / Q000209:etiology / Q000378:metabolism* / Q000628:therapy*; D010766:Phosphorylation; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D011972:Receptor, Insulin / Q000378:metabolism
chemical_list: D002096:C-Peptide; D007328:Insulin; D011972:Receptor, Insulin; D051057:Proto-Oncogene Proteins c-akt; D055372:AMP-Activated Protein Kinases; D007339:Insulysin; D005947:Glucose
keywords: high-fat diet; hyperinsulinemia; insulin degrading enzyme; obesity