title: Abscisic acid influx into human nucleated cells occurs through the anion exchanger AE2.
abstract: Abscisic acid (ABA) is a hormone conserved from cyanobacteria to higher plants, where it regulates responses to environmental stimuli. ABA also plays a role in mammalian physiology, pointedly in inflammatory responses and in glycemic control. As the animal ABA receptor is on the intracellular side of the plasma membrane, a transporter is required for the hormone's action. Here we demonstrate that ABA transport in human nucleated cells occurs via the anion exchanger AE2. Together with the recent demonstration that ABA influx into human erythrocytes occurs via Band 3, this result identifies the AE family members as the mammalian ABA transporters.
authors.affiliation: Department of Experimental Medicine, Section of Biochemistry, University of Genova, Viale Benedetto XV 1, Genova 16132, Italy. Electronic address: tiziana.vigliarolo@edu.unige.it.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Vigliarolo
authors.affiliation: Department of Experimental Medicine, Section of Biochemistry, University of Genova, Viale Benedetto XV 1, Genova 16132, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Zocchi
authors.affiliation: Department of Experimental Medicine, Section of Biochemistry, University of Genova, Viale Benedetto XV 1, Genova 16132, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Fresia
authors.affiliation: Department of Experimental Medicine, Section of Biochemistry, University of Genova, Viale Benedetto XV 1, Genova 16132, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Booz
authors.affiliation: Department of Experimental Medicine, Section of Biochemistry, University of Genova, Viale Benedetto XV 1, Genova 16132, Italy.
authors.forename: Lucrezia
authors.initials: L
authors.lastname: Guida
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D001692:Biological Transport; D027963:Chloride-Bicarbonate Antiporters / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism; D005786:Gene Expression Regulation; D020868:Gene Silencing; D006801:Humans; D020014:K562 Cells; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics; D009569:Nitric Oxide / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics; D028044:Phosphate-Binding Proteins; D013431:Sulfates / Q000378:metabolism
chemical_list: D027963:Chloride-Bicarbonate Antiporters; D002712:Chlorides; C471704:LANCL2 protein, human; D008565:Membrane Proteins; D009687:Nuclear Proteins; D028044:Phosphate-Binding Proteins; D013431:Sulfates; D009569:Nitric Oxide; D000040:Abscisic Acid
keywords: AE2-siRNA; Abscisic acid (ABA); Anion exchanger proteins (AE); K562 cells; Transport