title: [The pilot study on the expression of PHF8, H3K9me2, BDNF and LTP in the hippocampus of rats exposed to aluminum].
abstract: OBJECTIVE
  In this research, we have observed changes of PHF8、H3K9me2、BDNF, and their regulatory roles in changing the amplitude value of LTP in hippocampus due to aluminum exposure so that we can discuss the impact on the learning and memory that caused by chronic aluminum exposure.
  
  
  METHODS
  Forty healthy SPF grade SD male rats were randomly divided into four groups by weight, including control group and low, medium, high dose aluminum exposed group, each group had 10 rats. The exposed rats drank water containing different doses of aluminum chloride (AlCl3) (2、12、72 mg/kg Al(3+)) for 90 d. We measured LTP in hippocampus by electrophysiological grapier and detected the expression of PHF8、H3K9me2、BDNF by western-blot.
  
  
  RESULTS
  Electrophysiological measurements shows that compared with that of control group, the average of fEPSPs was decreased at different time points in all exposed groups (P<0.01) . The results of western-bolt test demonstrated that the expression of PHF8 in the exposed groups were significantly lower than those of control group (P<0.01) . And the expression the of H3K9me2 of medium and high dose groups were significantly higher than control group (P<0.05) . While the expression of BDNF of medium and high dose groups were decreased compared with the control group (P<0.05) .
  
  
  CONCLUSIONS
  Chronic aluminum exposure can reduce the LTP via the route of PHF8-H3K9me2-BDNF in the hippocampus of rats, which then may impair the ability of learning and memory.
authors.affiliation: Department of Occupational Health, Public Health of colleage, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Li
authors.forename: Pan
authors.initials: P
authors.lastname: Kang
authors.forename: H uifang
authors.initials: Hu
authors.lastname: Zhang
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Nie
authors.forename: Yuzhou
authors.initials: Y
authors.lastname: Yuan
authors.forename: Qiao
authors.initials: Q
authors.lastname: Niu
mesh_terms: D000535:Aluminum / Q000633:toxicity*; D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000633:toxicity; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D002712:Chlorides / Q000633:toxicity; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D056466:Histone Demethylases / Q000378:metabolism*; D007858:Learning / Q000187:drug effects; D017774:Long-Term Potentiation / Q000187:drug effects*; D008297:Male; D008568:Memory / Q000187:drug effects; D010865:Pilot Projects; D051381:Rats; D017207:Rats, Sprague-Dawley; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D017607:Aluminum Compounds; D019208:Brain-Derived Neurotrophic Factor; D002712:Chlorides; D014157:Transcription Factors; D000077410:Aluminum Chloride; D000535:Aluminum; D056466:Histone Demethylases