title: Observation of liposomes of differing lipid composition in aqueous medium by means of atomic force microscopy.
abstract: Liposomes present a challenge for atomic force microscopy (AFM) observation in aqueous medium because they easily collapse. Here, we demonstrate that bovine serum albumin coating of a glass substrate enables AFM observation of various liposomes in aqueous medium. With this AFM system, liposomes can be systematically observed and morphologically analyzed regardless of their surface charge, phase state, degree of lipid acyl chain unsaturation or PEG modification. This system thus has the potential to reveal the mechanical properties of liposomes of various lipid types and contents.
authors.affiliation: Japan Agency for Medical Research and Development, 1-7-1 Otemachi, Chiyoda-ku, Tokyo 100-0004, Japan Division of Drugs, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Takechi-Haraya
authors.affiliation: Division of Drugs, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan kumikato@nihs.go.jp.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Sakai-Kato
authors.affiliation: Division of Drugs, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Abe
authors.affiliation: National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Kawanishi
authors.affiliation: National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Haruhiro
authors.initials: H
authors.lastname: Okuda
authors.affiliation: Division of Drugs, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Goda
mesh_terms: D008051:Lipid Bilayers / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000648:ultrastructure*; D018625:Microscopy, Atomic Force / Q000379:methods*; D012709:Serum Albumin / Q000737:chemistry; D013499:Surface Properties; D014867:Water / Q000737:chemistry*
chemical_list: D008051:Lipid Bilayers; D008055:Lipids; D008081:Liposomes; D012709:Serum Albumin; D014867:Water
keywords: atomic force microscopy; bovine serum albumin; dynamic light scattering; liposome; membrane phase state; supported lipid bilayer