title: Evidence for Metabolic Pyrethroid Resistance in the Common Bed Bug (Hemiptera: Cimicidae).
abstract: Resistance to insecticides, especially the pyrethroids, in the common bed bug, Cimex lectularius L., has been well-documented. However, the presence and relative contribution of metabolic detoxifying microsomal oxidases and hydrolytic esterases to the observed resistance has yet to be fully elucidated. This is due, in part, to the absence of a simple bioassay procedure that appropriately isolates esterases from potentially competing oxidases. Recently, an analogue of piperonyl butoxide (PBO) was developed, EN16/5-1 (6-[2-(2-butoxyethoxy)ethoxymethyl]-5-propyl-2,3-dihydrobenzofuranby), which inhibits esterases but has limited efficacy against the oxidases, whereas PBO inhibits both. The opportunity is now available to use both synergists via established bioassay methodologies and to screen for the potential presence of oxidase- or esterase-derived pyrethroid resistance in insecticide-resistant insects, including bed bugs. In the present study, EN16/5-1 and PBO were assayed in conjunction with deltamethrin against four field strains of C. lectularius collected from independent geographic locations across Australia. All strains expressed a high degree of resistance to deltamethrin and significant inhibition of the observed resistance with preexposure to PBO. Nonsignificant differences between the cumulative mortality values for PBO and EN16/5-1 were then observed in two of the four bed bug strains, which indicate that detoxifying esterases are conferring substantially to the observed resistance in those strains. This study is the first to provide evidence that metabolic detoxification in the form of both hydrolytic esterases and microsomal oxidases is a major contributing factor to pyrethroid resistance in C. lectularius.
authors.forename: David G
authors.initials: DG
authors.lastname: Lilly
authors.forename: Kai
authors.initials: K
authors.lastname: Dang
authors.forename: Cameron E
authors.initials: CE
authors.lastname: Webb
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Doggett
keywords: Cimex; P450; bed bug; insecticide; pesticide