title: Polyketide Production of Pestaloficiols and Macrodiolide Ficiolides Revealed by Manipulations of Epigenetic Regulators in an Endophytic Fungus.
abstract: Regarding targeted disruption of epigenetic regulators, histone methyltransferase and deacetylase in a plant endophytic fungus Pestalotiopsis fici have been uncovered as an unexplored chemical repertoire. Manipulation of epigenetic regulators led to the isolation of 15 new polyketides, including pestaloficiols T-W (1-3 and 5), as well as 11 macrodiolide ficiolides A-K (6-16). Ficiolide K (16) was found to contain a very rare 1,6-anhydro-pyranose moiety. Finally, the biosynthetic origin of macrodiolide was characterized by isotope-labeling experiments.
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences , Beijing, 100101, China.
authors.forename: Guangwei
authors.initials: G
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences , Beijing, 100101, China.
authors.forename: Haichuan
authors.initials: H
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences , Beijing, 100101, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences , Beijing, 100101, China.
authors.forename: Xiuna
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences , Beijing, 100101, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences , Beijing, 100101, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences , Beijing, 100101, China.
authors.forename: Xingzhong
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences , Beijing, 100101, China.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Liu
authors.affiliation: Department of Medical Microbiology and Immunology, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.
authors.forename: Nancy P
authors.initials: NP
authors.lastname: Keller
authors.affiliation: Texas Therapeutics Institute, the Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center at Houston , Houston, Texas 77030, United States.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: An
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences , Beijing, 100101, China.
authors.forename: Wen-Bing
authors.initials: WB
authors.lastname: Yin
mesh_terms: D001688:Biological Products; D057890:Epigenomics; D005658:Fungi / Q000737:chemistry*; D007553:Isotope Labeling; D008358:Mannose / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D015394:Molecular Structure; D009005:Monosaccharides / Q000737:chemistry*; D061065:Polyketides / Q000737:chemistry*; D013098:Xylariales / Q000737:chemistry*
chemical_list: D001688:Biological Products; D009005:Monosaccharides; D061065:Polyketides; C000611015:ficiolide K; D008358:Mannose