title: Temperature Change Induces the Expression of vuuA Encoding Vulnibactin Receptor and crp Encoding Cyclic AMP Receptor Protein in Vibrio vulnificus.
abstract: Upon entering the human body, Vibrio vulnificus, a gram-negative marine bacterium, must withstand a temperature change (TC) from 25 to 37 °C. This bacterium acquires iron mainly via the vulnibactin receptor (VuuA)-mediated iron uptake system (IUS), which is under the positive control of cyclic AMP receptor protein (CRP), a global regulator responsible for catabolite repression. In this study, we examined the effect of TC on the expression of vuuA and crp, and the reciprocal relation between VuuA-mediated IUS and CRP under iron-limited conditions. Iron limitation increased vuuA expression but decreased crp expression. TC resulted in increased vuuA and crp expression. A crp or vuuA mutation reciprocally decreased vuuA or crp expression. TC could increase vuuA or crp expression even in a crp- or vuuA-mutated background. These results indicate that TC increases the expression of both vuuA and crp by facilitating metabolism under iron-limited conditions, and that CRP and VuuA-mediated IUS interact coordinately toward optimal metabolism in V. vulnificus.
authors.affiliation: Premedical Sciences, Chosun University Medical School, Gwangju, 501-759, Republic of Korea.
authors.forename: Choon-Mee
authors.initials: CM
authors.lastname: Kim
authors.affiliation: Premedical Sciences, Chosun University Medical School, Gwangju, 501-759, Republic of Korea.|Department of Medical Education, Chosun University Medical School, Gwangju, 501-759, Republic of Korea.
authors.forename: Young-Joon
authors.initials: YJ
authors.lastname: Ahn
authors.affiliation: Department of Emergency Medicine, Chosun University Medical School, Gwangju, 501-759, Republic of Korea.
authors.forename: Seong-Jung
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Department of Emergency Medicine, Chosun University Medical School, Gwangju, 501-759, Republic of Korea.
authors.forename: Dae-Heung
authors.initials: DH
authors.lastname: Yoon
authors.affiliation: Premedical Sciences, Chosun University Medical School, Gwangju, 501-759, Republic of Korea. shsin@chosun.ac.kr.|Department of Microbiology, Chosun University Medical School, 375 Seosuk-Dong, Dong-Gu, Gwangju, 501-759, Republic of Korea. shsin@chosun.ac.kr.
authors.forename: Sung-Heui
authors.initials: SH
authors.lastname: Shin
mesh_terms: D000577:Amides / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002373:Cyclic AMP Receptor Protein / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D010080:Oxazoles / Q000378:metabolism*; D013696:Temperature; D014735:Vibrio Infections / Q000382:microbiology; D041261:Vibrio vulnificus / Q000235:genetics / Q000378:metabolism*
chemical_list: D000577:Amides; D001426:Bacterial Proteins; D002373:Cyclic AMP Receptor Protein; D010080:Oxazoles; C086667:vulnibactin