title: Cisplatin Prodrug-Conjugated Gold Nanocluster for Fluorescence Imaging and Targeted Therapy of the Breast Cancer.
abstract: Theranostic nanomedicine has emerged as a promising modality for cancer diagnosis and treatment. In this study, we report the fabrication of fluorescence gold nanoclusters (GNC) conjugated with a cisplatin prodrug and folic acid (FA) (FA-GNC-Pt) for fluorescence imaging and targeted chemotherapy of breast cancer. The physio-chemical properties of FA-GNC-Pt nanoparticles are thoroughly characterized by fluorescence/UV-Vis spectroscopic measurement, particle size and zeta-potential examination. We find that FA-modification significantly accelerated the cellular uptake and increased the cytotoxicity of GNC-Pt nanoparticles in murine 4T1 breast cancer cells. Fluorescence imaging in vivo using 4T1 tumor bearing nude mouse model shows that FA-GNC-Pt nanoparticles selectively accumulate in the orthotopic 4T1 tumor and generate strong fluorescence signal due to the tumor targeting effect of FA. Moreover, we demonstrate that FA-GNC-Pt nanoparticles significantly inhibit the growth and lung metastasis of the orthotopically implanted 4T1 breast tumors. All these data imply a good potential of the GNC-based theranostic nanoplatform for fluorescence tumor imaging and cancer therapy.
authors.affiliation: 1. State Key Laboratory of Drug Research & Center of Pharmaceutics, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;; 2. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Fangyuan
authors.initials: F
authors.lastname: Zhou
authors.affiliation: 1. State Key Laboratory of Drug Research & Center of Pharmaceutics, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;
authors.forename: Bing
authors.initials: B
authors.lastname: Feng
authors.affiliation: 1. State Key Laboratory of Drug Research & Center of Pharmaceutics, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;
authors.forename: Haijun
authors.initials: H
authors.lastname: Yu
authors.affiliation: 1. State Key Laboratory of Drug Research & Center of Pharmaceutics, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;
authors.forename: Dangge
authors.initials: D
authors.lastname: Wang
authors.affiliation: 1. State Key Laboratory of Drug Research & Center of Pharmaceutics, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;
authors.forename: Tingting
authors.initials: T
authors.lastname: Wang
authors.affiliation: 1. State Key Laboratory of Drug Research & Center of Pharmaceutics, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;
authors.forename: Jianping
authors.initials: J
authors.lastname: Liu
authors.affiliation: 1. State Key Laboratory of Drug Research & Center of Pharmaceutics, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;
authors.forename: Qingshuo
authors.initials: Q
authors.lastname: Meng
authors.affiliation: 2. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Siling
authors.initials: S
authors.lastname: Wang
authors.affiliation: 1. State Key Laboratory of Drug Research & Center of Pharmaceutics, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: 1. State Key Laboratory of Drug Research & Center of Pharmaceutics, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: 1. State Key Laboratory of Drug Research & Center of Pharmaceutics, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;
authors.forename: Yaping
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000188:drug therapy*; D002945:Cisplatin / Q000008:administration & dosage* / Q000737:chemistry; D004195:Disease Models, Animal; D006046:Gold / Q000008:administration & dosage* / Q000737:chemistry; D008175:Lung Neoplasms / Q000517:prevention & control / Q000556:secondary; D008819:Mice, Nude; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D009362:Neoplasm Metastasis / Q000517:prevention & control; D061848:Optical Imaging / Q000379:methods; D011355:Prodrugs / Q000008:administration & dosage* / Q000737:chemistry; D000068936:Theranostic Nanomedicine / Q000379:methods; D016896:Treatment Outcome
chemical_list: D000970:Antineoplastic Agents; D011355:Prodrugs; D006046:Gold; D002945:Cisplatin
keywords: Fluorescence Imaging; Gold Nanoclusters; Targeted Therapy.; Theranostic Nanomedicine