title: Contribution of Human Fibroblasts and Endothelial Cells to the Hallmarks of Inflammation as Determined by Proteome Profiling.
abstract: In order to systematically analyze proteins fulfilling effector functionalities during inflammation, here we present a comprehensive proteome study of inflammatory activated primary human endothelial cells and fibroblasts. Cells were stimulated with interleukin 1-β and fractionated in order to obtain secreted, cytoplasmic and nuclear protein fractions. Proteins were submitted to a data-dependent bottom up analytical platform using a QExactive orbitrap and the MaxQuant software for protein identification and label-free quantification. Results were further combined with similarly generated data previously obtained from the analysis of inflammatory activated peripheral blood mononuclear cells. Applying a false discovery rate of less than 0.01 at both, peptide and protein level, a total of 8370 protein groups assembled from 117,599 peptides was identified; mass spectrometry data have been made fully accessible via ProteomeXchange with identifier PXD003406 to PXD003417.Comparative proteome analysis allowed us to determine common and cell type-specific inflammation signatures comprising novel candidate marker molecules and related expression patterns of transcription factors. Cardinal features of inflammation such as interleukin 1-β processing and the interferon response differed substantially between the investigated cells. Furthermore, cells also exerted similar inflammation-related tasks; however, by making use of different sets of proteins. Hallmarks of inflammation thus emerged, including angiogenesis, extracellular matrix reorganization, adaptive and innate immune responses, oxidative stress response, cell proliferation and differentiation, cell adhesion and migration in addition to monosaccharide metabolic processes, representing both, common and cell type-specific responsibilities of cells during inflammation.
authors.affiliation: From the ‡Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Austria.
authors.forename: Astrid
authors.identifier: 0000-0002-2217-5800
authors.initials: A
authors.lastname: Slany
authors.affiliation: From the ‡Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Austria.
authors.forename: Andrea
authors.initials: A
authors.lastname: Bileck
authors.affiliation: From the ‡Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Austria.
authors.forename: Dominique
authors.initials: D
authors.lastname: Kreutz
authors.affiliation: From the ‡Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Austria.
authors.forename: Rupert L
authors.initials: RL
authors.lastname: Mayer
authors.affiliation: From the ‡Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Austria.
authors.forename: Besnik
authors.initials: B
authors.lastname: Muqaku
authors.affiliation: From the ‡Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Austria christopher.gerner@univie.ac.at.
authors.forename: Christopher
authors.identifier: 0000-0003-4964-0642
authors.initials: C
authors.lastname: Gerner
mesh_terms: D002458:Cell Fractionation; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D053263:Gene Regulatory Networks / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism*; D053583:Interleukin-1beta / Q000494:pharmacology*; D013058:Mass Spectrometry / Q000379:methods; D020543:Proteome / Q000187:drug effects*; D040901:Proteomics / Q000379:methods
chemical_list: D053583:Interleukin-1beta; D020543:Proteome