title: Characterization and primary functional analysis of a bamboo NAC gene targeted by miR164b.
abstract: PeSNAC1 , a stress-related NAC1 from Phyllostachys edulis , was characterized. Ectopic expression in Arabidopsis indicated that PeSNAC1 together with ped -miR164b participated in the regulation of organ boundaries and stress tolerance. NAC (NAM, ATAF1/2 and CUC2) participates in many different processes regulating plant growth, development, and stress response. A total of 125 NAC genes have been predicted in moso bamboo (Phyllostachys edulis), but their roles are poorly understood. PeSNAC1 targeted by ped-miR164b was focused for further study. The cleavage of PeSNAC1 mRNA guided by ped-miR164b was validated using RLM-5' RACE. Tissue-specific expression analysis demonstrated that ped-miR164b had a declining trend from root, sheath, leaf, to that of stem, which was opposite to that of PeSNAC1. Transgenic Arabidopsis plants overexpressing either PeSNAC1 (OX-PeSNAC1) or, ped-miR164b (OX-ped-miR164b) driven by the CaMV35S promoter were generated. OX-ped-miR164b plants showed similar phenotype of cuc2 mutants whose growth was seriously suppressed. Compared with Col-0, sense OX-PeSNAC1 plants grew rapidly and flowered earlier, whereas antisense plants grew slowly and exhibited delayed flowering. Sense OX-PeSNAC1 plants had the greatest number of lateral roots, while antisense OX-PeSNAC1 and OX-ped-miR164b plants had fewer lateral roots than Col-0. Under NaCl and PEG6000 stresses, survival rates were higher and F v/F m values declined more slowly in sense OX-PeSNAC1 plants than in Col-0, with lower survival rates and a more rapid decrease in F v/F m values conversely observed in antisense OX-PeSNAC1 and OX-ped-miR164b plants. These findings indicated that ped-miR164b-targeted PeSNAC1 may play key roles in plant development and tolerance to salinity and drought stresses.
authors.affiliation: State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, Institute of Gene Science for Bamboo and Rattan Resources, International Center for Bamboo and Rattan, Beijing, 100102, China.|Lianyungang Academy of Agricultural Sciences, Lianyungang, 222000, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, Institute of Gene Science for Bamboo and Rattan Resources, International Center for Bamboo and Rattan, Beijing, 100102, China.
authors.forename: Hansheng
authors.initials: H
authors.lastname: Zhao
authors.affiliation: State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, Institute of Gene Science for Bamboo and Rattan Resources, International Center for Bamboo and Rattan, Beijing, 100102, China.|Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.
authors.forename: Dongliang
authors.initials: D
authors.lastname: Chen
authors.affiliation: State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, Institute of Gene Science for Bamboo and Rattan Resources, International Center for Bamboo and Rattan, Beijing, 100102, China.
authors.forename: Lichao
authors.initials: L
authors.lastname: Li
authors.affiliation: State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, Institute of Gene Science for Bamboo and Rattan Resources, International Center for Bamboo and Rattan, Beijing, 100102, China.
authors.forename: Huayu
authors.initials: H
authors.lastname: Sun
authors.affiliation: State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, Institute of Gene Science for Bamboo and Rattan Resources, International Center for Bamboo and Rattan, Beijing, 100102, China.
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Lou
authors.affiliation: State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, Institute of Gene Science for Bamboo and Rattan Resources, International Center for Bamboo and Rattan, Beijing, 100102, China. gaozhimin@icbr.ac.cn.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Gao
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D003681:Dehydration / Q000235:genetics / Q000503:physiopathology; D000066630:Ectopic Gene Expression / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D017343:Genes, Plant / Q000235:genetics / Q000502:physiology*; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology; D018517:Plant Roots / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000502:physiology; D060888:Real-Time Polymerase Chain Reaction; D055049:Salt Tolerance / Q000235:genetics / Q000502:physiology; D031787:Sasa / Q000235:genetics* / Q000502:physiology
chemical_list: D035683:MicroRNAs
keywords: Bamboo; F v/F m; Lateral root; NAC; NaCl and drought stresses; RLM-5′ RACE; miR164b; qRT-PCR