title: Expression of 3β-hydroxysteroid dehydrogenase in ovarian and uterine tissue during diestrus and open cervix cystic endometrial hyperplasia-pyometra in the bitch.
abstract: The purpose of this study was to compare the expression of 3β-hydroxystreroid dehydrogenase (3β-HSD) in the uterus and ovary of healthy dogs and those with cystic endometrial hyperplasia and/or pyometra complex (CEH-pyometra). Eighteen female dogs were included in the study. Eleven bitches with open cervix CEH-pyometra were included in the CEH-pyometra group and seven diestrus bitches in the control group. For immunostaining a rabbit polyclonal, one raised against recombinant human type 2 (adrenal/gonadal) 3β-HSD was used. Progesterone (P4) concentrations were not statistically different between the groups. Strongly stained large interstitial cell groups in the ovarian medulla were observed particularly in CEH-pyometra group although these cells in the control group were weakly or moderately stained and existed singly or paired. The expressions of 3β-HSD in luminal epithelium (42.40 ± 22.40% vs. 18.42 ± 13.15%, P < 0.05) and glandular epithelium (32.80 ± 27.05% vs. 2.94 ± 7.79%, P < 0.01) of endometrium were significantly higher in CEH-pyometra group than those in the control group. The expression of 3β-HSD in CL was higher (29.38 ± 9.58% vs. 22.94 ± 4.97%) in CEH-pyometra group than that of control group although the differences were not significant (P > 0.05). Similarly, the significant increase in the expression of 3β-HSD in ovarian interstitial cells (33.86 ± 29.44 vs. 1.13 ± 2.97, P < 0.05) was found in CEH-pyometra group compared to the control group. The study revealed that 3β-HSD expression in the endometrium of canine CEH-pyometra was significantly high.
authors.affiliation: Department of Obstetric and Gynecology, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun, Turkey. Electronic address: vetnilgun@gmail.com.
authors.forename: Nilgun
authors.initials: N
authors.lastname: Gultiken
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Yarim
authors.affiliation: Department of Biochemistry, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun, Turkey.
authors.forename: Gul Fatma
authors.initials: GF
authors.lastname: Yarim
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun, Turkey.
authors.forename: Ayhan
authors.initials: A
authors.lastname: Gacar
authors.affiliation: MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh, UK.
authors.forename: James Ian
authors.initials: JI
authors.lastname: Mason
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D004031:Diestrus / Q000502:physiology*; D004283:Dog Diseases / Q000378:metabolism / Q000473:pathology; D004285:Dogs; D004714:Endometrial Hyperplasia / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D005260:Female; D010053:Ovary / Q000378:metabolism*; D011374:Progesterone / Q000097:blood; D055112:Pyometra / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D014599:Uterus / Q000378:metabolism*
chemical_list: D011374:Progesterone; D015096:3-Hydroxysteroid Dehydrogenases
keywords: 3β-Hydroxysteroid dehydrogenase; Canine; Cystic endometrial hyperplasia; Pyometra