title: Existence of Paenibacillus larvae genotypes ERIC I-ST2, ERIC I-ST15 and ERIC II-ST10 in the western region of Aichi prefecture, Japan.
abstract: American foulbrood is the most destructive honeybee bacterial disease. The etiological agent, Paenibacillus larvae, has been classified into four genotypes by a repetitive-element PCR (ERIC I-IV) and 21 sequence types by multilocus sequence typing (ST1-21). In this study, we genotyped Japanese P. larvae isolates for the first time and revealed the presence of three genotypes (ERIC I-ST2, ERIC I-ST15 and ERIC II-ST10) in the western region of Aichi prefecture. ERIC I-ST15 and ERIC II-ST10 are globally distributed types, whereas the ERIC I-ST2 isolate was the first isolate of this genotype identified outside the native range of the European honeybee. The ERIC I and II isolates differed in phenotypes including cell morphology, and these may be useful for predicting ERIC types.
authors.affiliation: Aichi Prefectural Seibu Livestock Hygiene Service Center, Owari branch, 8-2673-5 Shinogi-cho, Kasugai, Aichi 486-0851, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Hirai
authors.forename: Toshinari
authors.initials: T
authors.lastname: Suzuki
authors.forename: Nanami
authors.initials: N
authors.lastname: Inaba
authors.forename: Naokazu
authors.initials: N
authors.lastname: Minoguchi
authors.forename: Daisuke
authors.initials: D
authors.lastname: Takamatsu
mesh_terms: D000818:Animals; D001516:Bees / Q000382:microbiology*; D005838:Genotype; D007564:Japan; D007814:Larva / Q000382:microbiology; D000070059:Paenibacillus larvae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*