title: Informing the Historical Record of Experimental Nonhuman Primate Infections with Ebola Virus: Genomic Characterization of USAMRIID Ebola Virus/H.sapiens-tc/COD/1995/Kikwit-9510621 Challenge Stock "R4368" and Its Replacement "R4415".
abstract: The creation of licensed medical countermeasures against Select Agents such as Ebola virus (EBOV) is critically dependent on the use of standardized reagents, assays, and animal models. We performed full genome reconstruction, population genomics, contaminant analysis, and characterization of the glycoprotein gene editing site of historical United States Army Medical Research Institute of Infectious Diseases (USAMRIID) nonhuman-primate challenge stock Ebola virus Kikwit "R4368" and its 2014 replacement "R4415." We also provide characterization of the master stock used to create "R4415." The obtained data are essential to understanding the quality of the seed stock reagents used in pivotal animal studies that have been used to inform medical countermeasure development. Furthermore, these data might add to the understanding of the influence of EBOV variant populations on pathogenesis and disease outcome and inform attempts to avoid the evolution of EBOV escape mutants in response to current therapeutics. Finally, as the primary challenge stocks have changed over time, these data will provide a baseline for understanding and correlating past and future animal study results.
authors.affiliation: United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, Frederick, Maryland, United States of America.
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Kugelman
authors.affiliation: United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, Frederick, Maryland, United States of America.
authors.forename: Cynthia A
authors.initials: CA
authors.lastname: Rossi
authors.affiliation: United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, Frederick, Maryland, United States of America.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Wiley
authors.affiliation: United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, Frederick, Maryland, United States of America.
authors.forename: Jason T
authors.initials: JT
authors.lastname: Ladner
authors.affiliation: United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, Frederick, Maryland, United States of America.
authors.forename: Elyse R
authors.initials: ER
authors.lastname: Nagle
authors.affiliation: United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, Frederick, Maryland, United States of America.
authors.forename: Bradley P
authors.initials: BP
authors.lastname: Pfeffer
authors.affiliation: United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, Frederick, Maryland, United States of America.
authors.forename: Karla
authors.initials: K
authors.lastname: Garcia
authors.affiliation: United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, Frederick, Maryland, United States of America.
authors.forename: Karla
authors.initials: K
authors.lastname: Prieto
authors.affiliation: Integrated Research Facility at Fort Detrick (IRF-Frederick), National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland, United States of America.
authors.forename: Jiro
authors.initials: J
authors.lastname: Wada
authors.affiliation: Integrated Research Facility at Fort Detrick (IRF-Frederick), National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland, United States of America.
authors.forename: Jens H
authors.initials: JH
authors.lastname: Kuhn
authors.affiliation: United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, Frederick, Maryland, United States of America.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Palacios
mesh_terms: D000047:Academies and Institutes; D000818:Animals; D029043:Ebolavirus / Q000235:genetics*; D023281:Genomics / Q000379:methods; D006023:Glycoproteins / Q000235:genetics; D019142:Hemorrhagic Fever, Ebola / Q000821:virology*; D011323:Primates / Q000821:virology*; D014481:United States
chemical_list: D006023:Glycoproteins