title: [THE EFFECTS OF LYS-PLASMINOGEN ON HUMAN PLATELET SECRETION].
abstract: The effects of Lys-plasminoge on platelet α-granule secretion were studied. The level of P-selectin exposed on the surface of plasma membranes of washed human platelets was measured by flow cytometry as a market of α-granule secretion. It was shown that Lys-plasminogen facilitates partial release of α-granules, but impedes thrombin-induced platelet exocytosis. It is suggested that Lys-plasminogen may affect platelet secretion rather through interaction of its non-catalytic (kringle) domains with membrane receptors than due to contaminating plasmin activity. In contrast to Lys-form, native proenzyme (Glu-plasminogen) had no effects on α-granule releasing. Here, we provide the first experimental demonstration that Lys-form of plasminogen is able to modulate platelet α-granule secretion, and this effect can be considered as one of the plausible mechanisms of its anti-aggregating activity.
authors.forename: A A
authors.initials: AA
authors.lastname: Tykhomyrov
authors.forename: D D
authors.initials: DD
authors.lastname: Zhernosekov
authors.forename: Y M
authors.initials: YM
authors.lastname: Roka-Moya
authors.forename: S I
authors.initials: SI
authors.lastname: Diordieva
authors.forename: T V
authors.initials: TV
authors.lastname: Grinenko
mesh_terms: D007611:Aprotinin / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D003594:Cytoplasmic Granules / Q000187:drug effects* / Q000378:metabolism; D005089:Exocytosis / Q000187:drug effects; D015870:Gene Expression; D006490:Hemostatics / Q000494:pharmacology; D006801:Humans; D019007:P-Selectin / Q000235:genetics / Q000378:metabolism; D010446:Peptide Fragments / Q000494:pharmacology*; D010958:Plasminogen / Q000494:pharmacology*; D017434:Protein Structure, Tertiary; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D013917:Thrombin / Q000494:pharmacology
chemical_list: D006490:Hemostatics; D019007:P-Selectin; D010446:Peptide Fragments; D011956:Receptors, Cell Surface; C018998:lysyl-plasminogen; D010958:Plasminogen; D007611:Aprotinin; D013917:Thrombin