title: Identification and characterization of microsatellite markers in Penstemon scariosus (Plantaginaceae).
abstract: OBJECTIVE
  Penstemon scariosus var. albifluvis (Plantaginaceae) has been proposed to be federally listed as threatened due to its unique, geologically oil-rich habitat. Developing simple sequence repeat (SSR) markers to study its genetic diversity would be most useful.
  
  
  RESULTS
  Using genomic reduction in combination with next-generation sequencing, we identified SSR motifs with five to 15 perfect repeats in 1067 P. scariosus contigs. After multiple qualifying tests, 16 SSRs were selected for their robust polymorphic reliability across 12 taxa with as high as 21 alleles in a given taxon. With the exception of two monomorphic loci, the observed and expected heterozygosity values ranged from 0.083 to 1.000 and 0.398 to 0.920, respectively.
  
  
  CONCLUSIONS
  These microsatellite markers will directly aid in studies of the genetic diversity and relatedness of P. scariosus, P. comarrhenus, P. compactus, P. cyananthus var. cyananthus, P. fremontii var. fremontii, P. fremontii var. glabrescens, P. gibbensii, P. strictus, and P. subglaber.
authors.affiliation: Department of Plant and Wildlife Sciences, Brigham Young University, Provo, Utah 84602 USA.
authors.forename: Chris D
authors.initials: CD
authors.lastname: Anderson
authors.affiliation: Department of Plant and Wildlife Sciences, Brigham Young University, Provo, Utah 84602 USA.
authors.forename: Nathan J
authors.initials: NJ
authors.lastname: Ricks
authors.affiliation: Department of Plant and Wildlife Sciences, Brigham Young University, Provo, Utah 84602 USA.
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Farley
authors.affiliation: Department of Plant and Wildlife Sciences, Brigham Young University, Provo, Utah 84602 USA.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Maughan
authors.affiliation: Department of Plant and Wildlife Sciences, Brigham Young University, Provo, Utah 84602 USA.
authors.forename: Mikel R
authors.initials: MR
authors.lastname: Stevens
keywords: 454 sequencing; Penstemon scariosus var. albifluvis; Plantaginaceae; White River penstemon; cross-amplification; simple sequence repeat (SSR) markers