title: Determination of Liposomal Cisplatin by High-Performance Liquid Chromatography and Its Application in Pharmacokinetic Studies.
abstract: Liposomes have been employed as carriers for antineoplastic drugs to improve delivery. We describe an HPLC-UV method for determining cisplatin levels in liposomal and biological samples, which represents an attractive alternative to the widely used flame atomic absorption spectroscopy. Liposomal cisplatin was extracted from liposomes, plasma and tissue samples by using acetonitrile and separated on a Symmetry C18 column. The mobile phase was a mixture of water, methanol and acetonitrile, and detection was performed at 254 nm. The method was linear in the range of 0.5-10 µg/mL. Using this method, cisplatin concentration was measured in plasma, kidney, liver and tumor at different times post-administration of liposomal cisplatin. This method is proved suitable for measuring the levels of cisplatin encapsulated in a liposomal system, in plasma or tissue samples of experimental animals, after intravenous administration of liposomal cisplatin. Owing to the small plasma volume employed, a complete pharmacokinetic study can be done with a single animal.
authors.affiliation: Laboratorio de Farmacologia, Subdirección de Investigación Básica, Instituto Nacional de Cancerología (INCan), Av. San Fernando #22, Tlalpan 14000, México D.F. 22026, México Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, México D.F. 11340, México.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Toro-Córdova
authors.affiliation: Laboratorio de Farmacologia, Subdirección de Investigación Básica, Instituto Nacional de Cancerología (INCan), Av. San Fernando #22, Tlalpan 14000, México D.F. 22026, México.
authors.forename: Fabricio
authors.initials: F
authors.lastname: Ledezma-Gallegos
authors.affiliation: Laboratorio de Farmacologia, Subdirección de Investigación Básica, Instituto Nacional de Cancerología (INCan), Av. San Fernando #22, Tlalpan 14000, México D.F. 22026, México.
authors.forename: Laura
authors.initials: L
authors.lastname: Mondragon-Fuentes
authors.affiliation: Laboratorio de Farmacologia, Subdirección de Investigación Básica, Instituto Nacional de Cancerología (INCan), Av. San Fernando #22, Tlalpan 14000, México D.F. 22026, México.
authors.forename: Rafael
authors.initials: R
authors.lastname: Jurado
authors.affiliation: Instituto de Física, Universidad Nacional Autónoma de México, México D.F. 04510, México Unidad de Investigación Biomédica en Cáncer INCan-UNAM, Instituto Nacional de Cancerología, México D.F. 14080, México.
authors.forename: Luis A
authors.initials: LA
authors.lastname: Medina
authors.affiliation: Laboratorio de Farmacologia, Subdirección de Investigación Básica, Instituto Nacional de Cancerología (INCan), Av. San Fernando #22, Tlalpan 14000, México D.F. 22026, México.
authors.forename: Jazmin M
authors.initials: JM
authors.lastname: Pérez-Rojas
authors.affiliation: Laboratorio de Farmacologia, Subdirección de Investigación Básica, Instituto Nacional de Cancerología (INCan), Av. San Fernando #22, Tlalpan 14000, México D.F. 22026, México pgarcia_lopez@yahoo.com.mx.
authors.forename: Patricia
authors.initials: P
authors.lastname: Garcia-Lopez
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D002945:Cisplatin / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D008081:Liposomes / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D000970:Antineoplastic Agents; D008081:Liposomes; D002945:Cisplatin