title: Complete nucleotide sequences of a new bipartite begomovirus from Malvastrum sp. plants with bright yellow mosaic symptoms in South Texas.
abstract: Two isolates of a novel bipartite begomovirus, tentatively named malvastrum bright yellow mosaic virus (MaBYMV), were molecularly characterized from naturally infected plants of the genus Malvastrum showing bright yellow mosaic disease symptoms in South Texas. Six complete DNA-A and five DNA-B genome sequences of MaBYMV obtained from the isolates ranged in length from 2,608 to 2,609 nucleotides (nt) and 2,578 to 2,605 nt, respectively. Both genome segments shared a 178- to 180-nt common region. In pairwise comparisons, the complete DNA-A and DNA-B sequences of MaBYMV were most similar (87-88 % and 79-81 % identity, respectively) and phylogenetically related to the corresponding sequences of sida mosaic Sinaloa virus-[MX-Gua-06]. Further analysis revealed that MaBYMV is a putative recombinant virus, thus supporting the notion that malvaceous hosts may be influencing the evolution of several begomoviruses. The design of new diagnostic primers enabled the detection of MaBYMV in cohorts of Bemisia tabaci collected from symptomatic Malvastrum sp. plants, thus implicating whiteflies as potential vectors of the virus.
authors.affiliation: Department of Plant Pathology and Microbiology, Texas A&M AgriLife Research and Extension Center, Weslaco, TX, 78596, USA. alabi@tamu.edu.
authors.forename: Olufemi J
authors.identifier: http://orcid.org/0000-0002-2471-7052
authors.initials: OJ
authors.lastname: Alabi
authors.affiliation: South Texas College, McAllen, TX, 78501, USA.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Villegas
authors.affiliation: Department of Plant Pathology and Microbiology, Texas A&M AgriLife Research and Extension Center, Weslaco, TX, 78596, USA.
authors.forename: Lori
authors.initials: L
authors.lastname: Gregg
authors.affiliation: South Texas College, McAllen, TX, 78501, USA.
authors.forename: K Daniel
authors.initials: KD
authors.lastname: Murray
mesh_terms: D000818:Animals; D053839:Begomovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D006430:Hemiptera / Q000821:virology; D007303:Insect Vectors / Q000821:virology; D019660:Malvaceae / Q000821:virology; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D011995:Recombination, Genetic; D013781:Texas
chemical_list: D004279:DNA, Viral