title: Inhibitory potential of Buffalo (Bubalus bubalis) colostrum immunoglobulin G on Klebsiella pneumoniae.
abstract: The unique components of colostrum like free oligosaccharides and glycoconjugates are known to offer resistance to enzymatic digestion in the gastrointestinal tract and have the ability to inhibit the localized adherence of enteropathogens to the digestive tract of the neonates. In this context, we have evaluated the in vitro effect of buffalo colostrum immunoglobulin G on human pathogen Klebsiella pneumoniae, a predominant multidrug resistant pathogen associated with nasocomial infections. The investigation revealed growth inhibitory potential of immunoglobulin G in a dose dependent manner supported by scanning electron microscopic studies. The N-glycan enriched fraction of immunoglobulin G after PNGase treatment was found more effective, comparable to ampicillin than native immunoglobulin G supporting the fact that colostrum derived oligosaccharides is crucial and act as ideal substrates for undesirable and pathogenic bacteria. The MALDI TOF/TOF analysis confirmed the glycostructures of abundant N-glycans of immunoglobulin G exerting antibacterial activity. The proteomic analysis revealed variations between control and treated cells and expression of chemotaxis-CheY protein (14kDa) was evidenced in response to immunoglobulin G treatment. Hence, it would be interesting to investigate the mode of inhibition of multidrug-resistant K. pneumoniae by buffalo colostrum immunoglobulin G with the identification of a newly expressed signalling protein.
authors.affiliation: Department of Biotechnology, University of Mysore, Manasagangotri, Mysore 570 006, India.
authors.forename: Mamatha Bhanu
authors.initials: MB
authors.lastname: L S
authors.affiliation: Faculty of Advanced Life Science, Hokkaido University, Sapporo 001-0021, Japan.
authors.forename: S-I
authors.initials: SI
authors.lastname: Nishimura
authors.affiliation: Department of Biotechnology, University of Mysore, Manasagangotri, Mysore 570 006, India. Electronic address: hsa.uom@gmail.com.
authors.forename: Aparna
authors.initials: A
authors.lastname: H S
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002020:Buffaloes; D002240:Carbohydrate Sequence; D003126:Colostrum / Q000737:chemistry*; D005260:Female; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D006868:Hydrolysis; D007074:Immunoglobulin G / Q000737:chemistry* / Q000302:isolation & purification; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D000072236:Methyl-Accepting Chemotaxis Proteins / Q000235:genetics / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000900:Anti-Bacterial Agents; D007074:Immunoglobulin G; D000072236:Methyl-Accepting Chemotaxis Proteins; D009844:Oligosaccharides; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
keywords: Buffalo colostrum; Immunoglobulin G; Klebsiella pneumonia; MALDI-TOF; N-Glycans