title: Lipopolysaccharide transport and assembly at the outer membrane: the PEZ model.
abstract: Gram-negative bacteria have a double-membrane cellular envelope that enables them to colonize harsh environments and prevents the entry of many clinically available antibiotics. A main component of most outer membranes is lipopolysaccharide (LPS), a glycolipid containing several fatty acyl chains and up to hundreds of sugars that is synthesized in the cytoplasm. In the past two decades, the proteins that are responsible for transporting LPS across the cellular envelope and assembling it at the cell surface in Escherichia coli have been identified, but it remains unclear how they function. In this Review, we discuss recent advances in this area and present a model that explains how energy from the cytoplasm is used to power LPS transport across the cellular envelope to the cell surface.
authors.affiliation: Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA.|Department of Bio-system Pharmacology, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan.
authors.forename: Suguru
authors.initials: S
authors.lastname: Okuda
authors.affiliation: Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Sherman
authors.affiliation: Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Silhavy
authors.affiliation: Department of Microbiology, The Ohio State University, Columbus, Ohio 43210, USA.
authors.forename: Natividad
authors.initials: N
authors.lastname: Ruiz
authors.affiliation: Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA.|Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.|Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Kahne
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D001692:Biological Transport; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism; D008070:Lipopolysaccharides / Q000737:chemistry / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D008954:Models, Biological
chemical_list: D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D008070:Lipopolysaccharides