title: Multiplexed Analysis for Anti-Epidermal Growth Factor Receptor Tumor Cell Growth Inhibition Based on Quantum Dot Probes.
abstract: Quantum dot (QD) multicolor analysis was achieved by labeling the epidermal growth factor and labeling the antibody against the epidermal growth factor receptor (anti-EGFR) using different QDs. On the basis of the fluorescence intensity of the two types of QDs, the amount of epidermal growth factors (EGFs) in the cells and anti-EGFRs bound to EGFRs were analyzed. The functions of anti-EGFR in preventing HeLa cells from engulfing EGF and inhibiting overproliferation of the HeLa cells were also studied. Meanwhile, parallel analysis was conducted to analyze the heterogeneity of cells at the single-cell level using a single-cell array. This provides a novel approach for the multiplexed analysis for the anti-EGFR functions in cells together with the cell heterogeneity. It also lays a foundation for parallel analysis and detection, using various fluorescence probes, simultaneous tracking, and detection of multiple targets at an overall level or individual level, and multichannel analysis of drug effects.
authors.affiliation: State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University , Beijing, 100084, China.
authors.forename: Dahai
authors.initials: D
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University , Beijing, 100084, China.
authors.forename: Yiqiu
authors.initials: Y
authors.lastname: Xia
authors.affiliation: State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University , Beijing, 100084, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University , Beijing, 100084, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: You
mesh_terms: D019187:Cadmium Compounds / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D066246:ErbB Receptors / Q000037:antagonists & inhibitors* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D019187:Cadmium Compounds; D005456:Fluorescent Dyes; D018036:Selenium Compounds; C058667:cadmium selenide; D066246:ErbB Receptors