title: Hepatocyte nuclear factor 1 beta induces transformation and epithelial-to-mesenchymal transition.
abstract: Gene amplification can be a cause of cancer, and driver oncogenes have been often identified in amplified regions. However, comprehensive analysis of other genes coamplified with an oncogene is rarely performed. We focused on the 17q12-21 amplicon, which contains ERBB2. We established a screening system for oncogenic activity with the NMuMG epithelial cell line. We identified a homeobox gene, HNF1B, as a novel cooperative transforming gene. HNF1B induced cancerous phenotypes, which were enhanced by the coexpression of ERBB2, and induced epithelial-to-mesenchymal transition and invasive phenotypes. These results suggest that HNF1B is a novel oncogene that can work cooperatively with ERBB2.
authors.affiliation: Department of Life Science and Medical Bioscience, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.
authors.forename: Atsuka
authors.initials: A
authors.lastname: Matsui
authors.affiliation: Japan Biological Informatics Consortium (JBiC), Tokyo, Japan.|Research Institute for Science and Engineering, Waseda University, Tokyo, Japan.
authors.forename: Jiro
authors.initials: J
authors.lastname: Fujimoto
authors.affiliation: Japan Biological Informatics Consortium (JBiC), Tokyo, Japan.|Research Institute for Science and Engineering, Waseda University, Tokyo, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Ishikawa
authors.affiliation: Division of Gene Expression Analysis, Translational Research Center, Fukushima Medical University, Japan.
authors.forename: Emi
authors.initials: E
authors.lastname: Ito
authors.affiliation: Division of Transcriptome Analysis, Translational Research Center, Fukushima Medical University, Japan.|Quantitative Proteomics Team, Molecular Profiling Research Center for Drug Discovery (molprof), National Institute of Advanced Industrial Science and Technology (AIST), Tokyo, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Goshima
authors.affiliation: Division of Gene Expression Analysis, Translational Research Center, Fukushima Medical University, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Watanabe
authors.affiliation: Department of Life Science and Medical Bioscience, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.|Division of Gene Function Analysis, Translational Research Center, Fukushima Medical University, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Semba
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D001943:Breast Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000378:metabolism* / Q000473:pathology; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D051539:Hepatocyte Nuclear Factor 1-beta / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D009154:Mutation / Q000235:genetics; D009362:Neoplasm Metastasis; D010641:Phenotype; D011379:Prognosis; D016019:Survival Analysis; D014158:Transcription, Genetic
chemical_list: D015415:Biomarkers; D051539:Hepatocyte Nuclear Factor 1-beta
keywords: breast tumor; epithelial-to-mesenchymal transition; gene amplification; hepatocyte nuclear factor 1 beta; three-dimensional culture