title: Comparative evaluation of demineralization of radicular dentin with 17% ethylenediaminetetraacetic acid, 10% citric acid, and MTAD at different time intervals: An in vitro study.
abstract: BACKGROUND
  The smear layer has the capability to protect the bacteria within the dentinal tubules from intracanal medicament. After removal of the smear layer from infected root canals, it allows disinfection of the entire root canal. The smear layer compromising the seal between the root canal sealer and root canal wall also decreases the penetration of irrigants into dentinal tubules.
  
  
  OBJECTIVE
  This study compares the amount of phosphorous liberated and demineralization of the radicular dentin with 17% ethylenediaminetetraacetic acid, 10% citric acid and mixture of doxycycline, citric acid, and a detergent at different time intervals.
  
  
  METHODS
  Extracted maxillary single-rooted teeth were prepared by using a combination of passive step-back and rotary 0.04 taper nickel-titanium files. Sodium hypochlorite 5.25% and sterile distilled water were used as an intracanal irrigant. The canals were then treated with 5 mL of one of the following solutions such as final rinse sterile distilled water, 5.25% sodium hypochlorite, 17% ethylenediaminetetraacetic acid (EDTA) or mixture of doxycycline, citric acid, and a detergent. The presence or absence of smear layer and the amount of erosion on the surface of the root canal walls at the coronal, middle, and apical portions of each canal were examined under a scanning electron microscope.
  
  
  METHODS
  Data were analyzed by one-way analysis of variance (ANOVA) to determine whether there were significant differences between the groups.
  
  
  RESULTS
  The results show that mixture of doxycycline, citric acid, and a detergent is an effective solution for the removal of the smear layer and does not significantly change the structure of the dentinal tubules.
  
  
  CONCLUSIONS
  In this study, 10% citric acid shows the maximum amount of dimeneralization of radicular dentine followed by mixture of doxycycline, citric acid, and a detergent, and 17% ethylenediaminetetraacetic acid. When all the subgroups were compared, it was seen that the maximum amount of phosphorus liberation was performed by 10% citric acid >mixture of doxycycline, citric acid, and a detergent >17% EDTA at a different time interval.
authors.affiliation: Department of Conservative and Endodontics, Darshan Dental College and Hospital, Udaipur, Rajasthan, India.
authors.forename: Yogender
authors.initials: Y
authors.lastname: Kumar
authors.affiliation: Department of Conservative and Endodontics, Darshan Dental College and Hospital, Udaipur, Rajasthan, India.
authors.forename: Jitendra
authors.initials: J
authors.lastname: Lohar
authors.affiliation: Department of Conservative and Endodontics, Darshan Dental College and Hospital, Udaipur, Rajasthan, India.
authors.forename: Sureka
authors.initials: S
authors.lastname: Bhat
authors.affiliation: Department of Dentistry, Mathura Das Mathur Hospital, Jodhpur, Rajasthan, India.
authors.forename: Manisha
authors.initials: M
authors.lastname: Bhati
authors.affiliation: Department of Conservative and Endodontics, Darshan Dental College and Hospital, Udaipur, Rajasthan, India.
authors.forename: Aanesh
authors.initials: A
authors.lastname: Gandhi
authors.affiliation: Department of Conservative and Endodontics, Dashvani Dental College and Hospital, Kota, Rajasthan, India.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Mehta
keywords: Citric acid; MTAD; citric acid; detergent; ethylenediaminetetraacetic acid; mixture of doxycycline; smear layer