title: [Study on association between HLA-DP gene polymorphism and susceptibility to hepatitis B virus infection in minority population in Guizhou province].
abstract: OBJECTIVE
  To investigate the association between HLA-DP gene polymorphism and the susceptibility to hepatitis B virus (HBV) infection in people in Buyi, Miao and Shui ethnic groups in Guizhou.
  
  
  METHODS
  A total of 256 patients with HBV infection, 142 HBV self-cleared patients and 135 controls were recruited from 3 ethnic groups for this case-control study. Genotypes of rs3077 and rs9277535 were determined by using real-time PCR with a TaqMan-MGB probe.
  
  
  RESULTS
  Compared with healthy subjects and self-cleared subjects, the allele distribution of rs9277535 was significantly associated with chronic HBV infection (P<0.05), no significant differences in rs3077 allele and genotype distributions were found among 3 groups (P>0.05). Compared with the rs9277535 GG genotype, the AA and AG genotypes were protective factors against HBV infection in dominant model after adjustment for age and sex (OR=0.645, 95%CI: 0.421-0.988), and no significant difference in rs3077 locus was found in the same analysis (P>0.05). For men, the rs3077 locus CC and CT genotypes were also protective factors against HBV infection (OR=0.493, 95%CI: 0.266-0.916), and there was no significant difference in rs9277535 locus compared the genotype distributions among 3 groups in dominant mode. Comparison of allele and genotype distribution in 3 ethnic groups showed that HLA-DP gene rs3077 genotype distributions had significant difference between the HBV infection group and the healthy control group in Buyi ethnic group (χ(2)=6.726, P=0.036).
  
  
  CONCLUSIONS
  The presence of rs9277535A allele atHLA-DP gene polymorphisms might decrease the risk for HBV infection, the rs3077 C allele at HLA-DP gene polymorphisms might also confer protective effect against HBV infection in women. No significant correlation between HLA-DP gene rs3077 and rs9277535 locus polymorphism and HBV clearance was found in this study. The HLA-DP gene rs3077 genotype distribution of HBV-infected patients had significant differences among different ethnic groups.
authors.affiliation: Key Laboratory of Molecular Biology, Guizhou Medical University, Guiyang 550004, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Zhao
authors.forename: Yan
authors.initials: Y
authors.lastname: He
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.forename: Chanjuan
authors.initials: C
authors.lastname: Wang
authors.forename: Wangde
authors.initials: W
authors.lastname: Zhang
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Yu
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Guan
mesh_terms: D000483:Alleles; D016022:Case-Control Studies; D002681:China; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006682:HLA-DP Antigens / Q000235:genetics*; D006509:Hepatitis B / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000235:genetics; D006801:Humans; D008297:Male; D008913:Minority Groups / Q000706:statistics & numerical data*; D011110:Polymorphism, Genetic; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D006682:HLA-DP Antigens