title: (1)H, (15)N and (13)C backbone and side chain resonance assignments of the RRM domain from human RBM24.
abstract: Tissue development requires the expression of a regulated subset of genes, and it is becoming clear that the process of alternative splicing also plays an important role in the production of necessary tissue-specific isoforms. However, only a few of these tissue-specific splicing factors in mammals have so far been discovered. One of these factors is the RNA-binding protein RBM24 which has been recently identified as a major regulator of alternative splicing in cardiac and skeletal muscle development. The RBM24 protein contains an RNA recognition motif (RRM) domain that presumably mediates the binding to target pre-mRNA required for regulation of the splicing patterns. Here we report (1)H, (15)N and (13)C chemical shift assignments of the backbone and sidechain atoms for the RRM domain from human RBM24. Secondary chemical shift analysis and relaxation measurement confirm the canonical architecture of the RRM domain. The data will allow for atomic level studies aimed at understanding splicing regulation of target genes in heart and muscle development and investigation into a separate role of RBM24 in modulating mRNA stability of genes involved in the p53 tumor suppressor pathway.
authors.affiliation: CSIR-Institute of Genomics and Integrative Biology, New Delhi, 110020, India. skupadhyay@msn.com.
authors.forename: Santosh Kumar
authors.initials: SK
authors.lastname: Upadhyay
authors.affiliation: Institut Européen de Chimie et Biologie (IECB), University of Bordeaux, 2 rue Robert Escarpit, 33607, Pessac, France.|Inserm, U869, ARNA Laboratory, University of Bordeaux, 33076, Bordeaux, France.
authors.forename: Cameron D
authors.initials: CD
authors.lastname: Mackereth
mesh_terms: D020816:Amino Acid Motifs; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular; D000072417:Protein Domains; D012313:RNA / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: C000594453:RBM24 protein, human; D016601:RNA-Binding Proteins; D012313:RNA
keywords: Alternative splicing; Development; Heart; Muscle; mRNA