title: Separable Roles for a Caenorhabditis elegans RMI1 Homolog in Promoting and Antagonizing Meiotic Crossovers Ensure Faithful Chromosome Inheritance.
abstract: During the first meiotic division, crossovers (COs) between homologous chromosomes ensure their correct segregation. COs are produced by homologous recombination (HR)-mediated repair of programmed DNA double strand breaks (DSBs). As more DSBs are induced than COs, mechanisms are required to establish a regulated number of COs and to repair remaining intermediates as non-crossovers (NCOs). We show that the Caenorhabditis elegans RMI1 homolog-1 (RMH-1) functions during meiosis to promote both CO and NCO HR at appropriate chromosomal sites. RMH-1 accumulates at CO sites, dependent on known pro-CO factors, and acts to promote CO designation and enforce the CO outcome of HR-intermediate resolution. RMH-1 also localizes at NCO sites and functions in parallel with SMC-5 to antagonize excess HR-based connections between chromosomes. Moreover, RMH-1 also has a major role in channeling DSBs into an NCO HR outcome near the centers of chromosomes, thereby ensuring that COs form predominantly at off-center positions.
authors.affiliation: Department of Chromosome Biology, Max F. Perutz Laboratories, Vienna Bio Center, University of Vienna, Vienna, Austria.
authors.forename: Marlène
authors.initials: M
authors.lastname: Jagut
authors.affiliation: Department of Chromosome Biology, Max F. Perutz Laboratories, Vienna Bio Center, University of Vienna, Vienna, Austria.
authors.forename: Patricia
authors.initials: P
authors.lastname: Hamminger
authors.affiliation: Department of Chromosome Biology, Max F. Perutz Laboratories, Vienna Bio Center, University of Vienna, Vienna, Austria.|Departments of Developmental Biology and Genetics, Stanford University School of Medicine, Stanford, California, United States of America.
authors.forename: Alexander
authors.initials: A
authors.lastname: Woglar
authors.affiliation: Department of Chromosome Biology, Max F. Perutz Laboratories, Vienna Bio Center, University of Vienna, Vienna, Austria.
authors.forename: Sophia
authors.initials: S
authors.lastname: Millonigg
authors.affiliation: Center for Integrative Bioinformatics Vienna (CIBIV), Max F. Perutz Laboratories, Vienna Bio Center, University of Vienna and Medical University of Vienna, Vienna, Austria.
authors.forename: Luis
authors.initials: L
authors.lastname: Paulin
authors.affiliation: Department of Chromosome Biology, Max F. Perutz Laboratories, Vienna Bio Center, University of Vienna, Vienna, Austria.
authors.forename: Martin
authors.initials: M
authors.lastname: Mikl
authors.affiliation: Department of Chromosome Biology, Max F. Perutz Laboratories, Vienna Bio Center, University of Vienna, Vienna, Austria.
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Dello Stritto
authors.affiliation: Department of Chromosome Biology, Max F. Perutz Laboratories, Vienna Bio Center, University of Vienna, Vienna, Austria.
authors.forename: Lois
authors.initials: L
authors.lastname: Tang
authors.affiliation: Department of Chromosome Biology, Max F. Perutz Laboratories, Vienna Bio Center, University of Vienna, Vienna, Austria.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Habacher
authors.affiliation: Departments of Developmental Biology and Genetics, Stanford University School of Medicine, Stanford, California, United States of America.
authors.forename: Angela
authors.initials: A
authors.lastname: Tam
authors.affiliation: Center for Integrative Bioinformatics Vienna (CIBIV), Max F. Perutz Laboratories, Vienna Bio Center, University of Vienna and Medical University of Vienna, Vienna, Austria.
authors.forename: Miguel
authors.initials: M
authors.lastname: Gallach
authors.affiliation: Center for Integrative Bioinformatics Vienna (CIBIV), Max F. Perutz Laboratories, Vienna Bio Center, University of Vienna and Medical University of Vienna, Vienna, Austria.|Bioinformatics and Computational Biology, Faculty of Computer Science, University of Vienna, Austria.
authors.forename: Arndt
authors.initials: A
authors.lastname: von Haeseler
authors.affiliation: Departments of Developmental Biology and Genetics, Stanford University School of Medicine, Stanford, California, United States of America.
authors.forename: Anne M
authors.initials: AM
authors.lastname: Villeneuve
authors.affiliation: Department of Chromosome Biology, Max F. Perutz Laboratories, Vienna Bio Center, University of Vienna, Vienna, Austria.
authors.forename: Verena
authors.initials: V
authors.lastname: Jantsch
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D020090:Chromosome Segregation; D003434:Crossing Over, Genetic; D004720:Endonucleases / Q000378:metabolism; D009154:Mutation; D049471:Pachytene Stage
chemical_list: D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; C487047:HIM-6 protein, C elegans; C000609626:RMH-1 protein, C elegans; C000594909:SMC-5 protein, C elegans; D004720:Endonucleases