title: The Effect of 3',4'-Dihydroxyflavonol on Lipid Peroxidation in Rats with Cerebral Ischemia Reperfusion Injury.
abstract: The aim of present study was to determine the effect of 3',4'-dihydroxyflavonol (DiOHF) on lipid peroxidation in experimental brain ischemia-reperfusion in rats. Present study was performed on the 34 male Wistar-albino rats, weigth 350-400 g. Experiment groups were designed as 1-Sham; 2-Ischemia-reperfusion; animal were anesthesized and carotid arteried were clemped for 20 min and reperfusion (7 days). 3-DiOHF + Ischemia-reperfusion; DiOHF was given to animals as 10 mg/kg by intraperitoneal. 4- Ischemia + DiOHF + Reperfusion; 5- Ischemia-reperfusion + DiOHF. Blood samples and serebral cortex were analysed for malondyaldehyde (MDA), NO (nitric oxide), xanthine oxidase (XO), glutathione (GSH) and glutathione peroxidase (GPx). Blood MDA levels were significantly higher ischemia-reperfusion groups (P < 0.005). However, DiOHF inhibited MDA. Ischemia-reperfusion led to increased XO and NO but DiOHF supplementation reduced NO and XO. DiOHF increased GSH and GPx levels compared to ischemia-reperfusion group. All together, our present study showed that intraperitoneal DiOHF supplementation has protective effect on brain ischaemia-reperfusion injury in rat.
authors.affiliation: Department of Physiology, Faculty of Medicine, Selcuk University, Konya, Turkey.
authors.forename: Merve
authors.initials: M
authors.lastname: Caliskan
authors.affiliation: Department of Physiology, Faculty of Medicine, Selcuk University, Konya, Turkey. rasimmogulkoc@yahoo.com.
authors.forename: Rasim
authors.initials: R
authors.lastname: Mogulkoc
authors.affiliation: Department of Physiology, Faculty of Medicine, Selcuk University, Konya, Turkey.
authors.forename: Abdulkerim Kasim
authors.initials: AK
authors.lastname: Baltaci
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Selcuk University, Konya, Turkey.
authors.forename: Esma
authors.initials: E
authors.lastname: Menevse
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D002545:Brain Ischemia / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D044948:Flavonols / Q000494:pharmacology / Q000627:therapeutic use*; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D016896:Treatment Outcome
chemical_list: D044948:Flavonols; C529890:3',4'-dihydroxyflavonol
keywords: 3′,4′-dihydroxyflavonol; Brain ischaemia–reperfusion; Free radicals; Rat