title: Simultaneous analysis of seven marker compounds from Saposhnikoviae Radix, Glehniae Radix and Peucedani Japonici Radix by HPLC/PDA.
abstract: A new combination of high performance liquid chromatography (HPLC) method coupled with photodiode array (PDA) analysis has been developed for the simultaneous quantitative determination of seven major components in Saposhnikoviae Radix (SR), Glehniae Radix (GR) and Peucedani Japonici Radix (PR), namely peucedanol 7-O-β-D-glucopyranoside (1), prim-O-glucosylcimifugin (2), cimifugin (3), 4'-O-β-D-glucosyl-5-O-methylvisamminol (4), bergapten (5), sec-O-glucosylhamaudol (6), and imperatorin (7). Clear separation of these seven components were achieved on a Phenomenex Kinetex C18 (250 × 4.6 mm, 5 μm) column by gradient elution of water (A) and methanol (B) as mobile phase. The flow rate was 1.0 mL/min and the UV detector wavelength was set at 254 nm. The method was successfully used in the analysis of SR, GR, and PR with relatively simple conditions and procedures, and the results were satisfactory for linearity, recovery, precision, accuracy, stability and robustness. The results indicate that the established HPLC/PDA method is suitable for the classification of SR, GR, and PR.
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongsan, 38430, Republic of Korea.
authors.forename: U Min
authors.initials: UM
authors.lastname: Seo
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongsan, 38430, Republic of Korea.|Henan Institute of Science and Technology, Xinxiang, Henan, 453003, China.
authors.forename: Bing Tian
authors.initials: BT
authors.lastname: Zhao
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon, 34134, Republic of Korea.
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon, 34134, Republic of Korea.
authors.forename: Jong Seong
authors.initials: JS
authors.lastname: Kang
authors.affiliation: College of Pharmacy, Yeungnam University, Gyeongsan, 38541, Republic of Korea.
authors.forename: Jong Keun
authors.initials: JK
authors.lastname: Son
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongsan, 38430, Republic of Korea. woomh@cu.ac.kr.
authors.forename: Mi-Hee
authors.initials: MH
authors.lastname: Woo
mesh_terms: D019661:Apiaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000592:standards; D057230:Limit of Detection; D016014:Linear Models; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D004365:Drugs, Chinese Herbal
keywords: Glehniae Radix; HPLC/PDA; Pattern recognition; Peucedani Japonici Radix; Saposhnikoviae Radix; Simultaneous analysis