title: Inkjet-assisted layer-by-layer printing of quantum dot/enzyme microarrays for highly sensitive detection of organophosphorous pesticides.
abstract: We present a facile fabrication of layer-by-layer (LbL) microarrays of quantum dots (QDs) and acetylcholinesterase enzyme (AChE). The resulting arrays had several unique properties, such as low cost, high integration and excellent flexibility and time-saving. The presence of organophosphorous pesticides (OPs) can inhibit the AChE activity and thus changes the fluorescent intensity of QDs/AChE microscopic dot arrays. Therefore, the QDs/AChE microscopic dot arrays were used for the sensitive visual detection of OPs. Linear calibration for parathion and paraoxon was obtained in the range of 5-100 μg L(-1) under the optimized conditions with the limit of detection (LOD) of 10 μg L(-1). The arrays have been successfully used for detection of OPs in fruits and water real samples. The new array was validated by comparison with conventional high performance liquid chromatography-mass spectrometry (HPLC-MS).
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Life Science and Technology, Harbin Institute of Technology, Harbin 150080, China; Micro- and Nanotechnology Research Center, Harbin Institute of Technology, Harbin 150080, China.
authors.forename: Enxiao
authors.initials: E
authors.lastname: Luan
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Life Science and Technology, Harbin Institute of Technology, Harbin 150080, China; Micro- and Nanotechnology Research Center, Harbin Institute of Technology, Harbin 150080, China.
authors.forename: Zhaozhu
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Life Science and Technology, Harbin Institute of Technology, Harbin 150080, China; Micro- and Nanotechnology Research Center, Harbin Institute of Technology, Harbin 150080, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Life Science and Technology, Harbin Institute of Technology, Harbin 150080, China; Micro- and Nanotechnology Research Center, Harbin Institute of Technology, Harbin 150080, China.
authors.forename: Hongxi
authors.initials: H
authors.lastname: Gu
authors.affiliation: Micro- and Nanotechnology Research Center, Harbin Institute of Technology, Harbin 150080, China. Electronic address: shaoqinliu@hit.edu.cn.
authors.forename: Shaoqin
authors.initials: S
authors.lastname: Liu
mesh_terms: D004798:Enzymes / Q000737:chemistry*; D057230:Limit of Detection; D009943:Organophosphorus Compounds / Q000032:analysis*; D010575:Pesticides / Q000032:analysis*; D045663:Quantum Dots; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004798:Enzymes; D009943:Organophosphorus Compounds; D010575:Pesticides
keywords: Acetylcholinesterase; Biosensor; Inkjet-printing; Layer-by-layer assembly; Organophosphorus pesticide; Quantum dot