title: Cranial bone regeneration via BMP-2 encoding mesenchymal stem cells.
abstract: Cranial bone repair and regeneration via tissue engineering principles has attracted a great deal of interest from researchers during last decade. Here, within this study, 6 mm critical-sized bone defect regeneration via genetically modified mesenchymal stem cells (MSC) were monitored up to 4 months. Cranial bone repair and new bone formations were evaluated by histological staining and real time PCR analysis in five different groups including autograft and bone morphogenetic protein-2 (BMP-2) transfected MSC groups. Results presented here indicate a proper cranial regeneration in autograft groups and a prospering regeneration for hBMP-2 encoding mesenchymal stem cells.
authors.affiliation: a Department of Plastic and Aesthetic Surgery, Faculty of Medicine , Kirikkale University , Kirikkale , Turkey.
authors.forename: Altugan Cahit
authors.initials: AC
authors.lastname: Vural
authors.affiliation: b Department of Chemistry, Faculty of Science , Ankara University , Ankara , Turkey.
authors.forename: Sedat
authors.initials: S
authors.lastname: Odabas
authors.affiliation: c Department of Histology & Embryology, Faculty of Medicine , Hacettepe University , Ankara , Turkey.
authors.forename: Petek
authors.initials: P
authors.lastname: Korkusuz
authors.affiliation: d Department of Medical Biology and Genetics, Faculty of Medicine , Gazi University , Ankara , Turkey.
authors.forename: Atiye Seda
authors.initials: AS
authors.lastname: Yar Sağlam
authors.affiliation: c Department of Histology & Embryology, Faculty of Medicine , Hacettepe University , Ankara , Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Bilgiç
authors.affiliation: a Department of Plastic and Aesthetic Surgery, Faculty of Medicine , Kirikkale University , Kirikkale , Turkey.
authors.forename: Tarık
authors.initials: T
authors.lastname: Çavuşoğlu
authors.affiliation: e Chemical Engineering Department and Bioengineering Division, Center for Bioengineering - Biyomedtek , Hacettepe University , Ankara , Turkey.
authors.forename: Erhan
authors.initials: E
authors.lastname: Piskin
authors.affiliation: f Department of Plastic & Aesthetic Surgery, Faculty of Medicine , Hacettepe University , Ankara , Turkey.
authors.forename: İbrahim
authors.initials: İ
authors.lastname: Vargel
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics* / Q000378:metabolism; D001861:Bone Regeneration / Q000235:genetics*; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D005260:Female; D015870:Gene Expression; D005818:Genetic Engineering; D015552:Injections, Intralesional; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis / Q000235:genetics; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000293:injuries / Q000378:metabolism; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014162:Transfection; D019076:Transgenes; D014182:Transplantation, Autologous
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2
keywords: Bone morphogenetic protein-2; cranial bone regeneration; critical size defect; mesenchymal stem cell; transfection