title: Acquiring Structural Information on Virus Particles with Charge Detection Mass Spectrometry.
abstract: Charge detection mass spectrometry (CDMS) is a single-molecule technique particularly well-suited to measuring the mass and charge distributions of heterogeneous, MDa-sized ions. In this work, CDMS has been used to analyze the assembly products of two coat protein variants of bacteriophage P22. The assembly products show broad mass distributions extending from 5 to 15 MDa for A285Y and 5 to 25 MDa for A285T coat protein variants. Because the charge of large ions generated by electrospray ionization depends on their size, the charge can be used to distinguish hollow shells from more compact structures. A285T was found to form T = 4 and T = 7 procapsids, and A285Y makes a small number of T = 3 and T = 4 procapsids. Owing to the decreased stability of the A285Y and A285T particles, chemical cross-linking was required to stabilize them for electrospray CDMS.Graphical Abstract.
authors.affiliation: Department of Chemistry, Indiana University, Bloomington, IN, 47405, USA.
authors.forename: David Z
authors.initials: DZ
authors.lastname: Keifer
authors.affiliation: Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT, 06269, USA.
authors.forename: Tina
authors.initials: T
authors.lastname: Motwani
authors.affiliation: Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT, 06269, USA.|Department of Chemistry, University of Connecticut, Storrs, CT, 06269, USA.
authors.forename: Carolyn M
authors.initials: CM
authors.lastname: Teschke
authors.affiliation: Department of Chemistry, Indiana University, Bloomington, IN, 47405, USA. mfj@indiana.edu.
authors.forename: Martin F
authors.initials: MF
authors.lastname: Jarrold
mesh_terms: D002213:Capsid; D036022:Capsid Proteins / Q000737:chemistry*; D013058:Mass Spectrometry; D014771:Virion / Q000737:chemistry*; D019065:Virus Assembly
chemical_list: D036022:Capsid Proteins
keywords: Bacteriophage P22; Charge detection mass spectrometry; Coat protein; Procapsid; Virus capsid