title: A novel biocompatible conducting polyvinyl alcohol (PVA)-polyvinylpyrrolidone (PVP)-hydroxyapatite (HAP) composite scaffolds for probable biological application.
abstract: We have prepared biocompatible composites of 80wt% polyvinyl alcohol (PVA)-(20wt%) polyvinylpyrrolidone (PVP) blend with different concentrations of bioactive nanohydroxyapatite, Ca10(PO4)6(HO)2 (HAP). The composite films demonstrated maximum effective conductivity (σ∼1.64×10(-4)S/m) and effective dielectric constant (ε∼290) at percolation threshold concentration (∼10wt% HAP) at room temperature. These values of σ and ε are much higher than those of PVA, PVP or HAP. Our preliminary observation indicated excellent biocompatibility of the electrospun fibrous meshes of two of these composites with different HAP contents (8.5 and 5wt% within percolation threshold concentration) using NIH 3T3 fibroblast cell line. Cells viability on the well characterized composite fibrous scaffolds was determined by MTT [3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay analysis. Enhancement of σ, due to HAP addition, was found to show increased biocompatibility of the fibrous scaffold. Enhanced σ value of the PVA/PVP-HAP composite provided supporting cues for the increased cell viability and biocompatibility of the composite fibrous meshes. Excellent biocompatibility these electrospun composite scaffolds made them to plausible potential candidates for tissue engineering or other biomedical applications.
authors.affiliation: Centre for Rural & Cryogenic Technologies, Jadavpur University, Kolkata 700032, India. Electronic address: chaudhuri.bis12345@gmail.com.
authors.forename: B
authors.initials: B
authors.lastname: Chaudhuri
authors.affiliation: Central Scientific Service, Indian Association for the Cultivation of Science, Kolkata 700032, India.
authors.forename: B
authors.initials: B
authors.lastname: Mondal
authors.affiliation: Centre for Bioseparation Technology (CBST), VIT-University, Vellore, Tamil Nadu 632014, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Ray
authors.affiliation: Centre for Rural & Cryogenic Technologies, Jadavpur University, Kolkata 700032, India.
authors.forename: S C
authors.initials: SC
authors.lastname: Sarkar
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D017886:Durapatite / Q000737:chemistry* / Q000494:pharmacology; D004553:Electric Conductivity; D055664:Electrochemical Techniques; D051379:Mice; D041681:NIH 3T3 Cells; D053761:Nanocomposites / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry* / Q000494:pharmacology; D016062:Porosity; D011205:Povidone / Q000737:chemistry* / Q000494:pharmacology; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D011142:Polyvinyl Alcohol; D017886:Durapatite; D011205:Povidone
keywords: Biocompatibility; Biomedical applications; Conducting polymer; Electrical conductivity; Electrospinning; Hydroxyapatite; Polymer composite