title: The key role of CYC2 during meiosis in Tetrahymena thermophila.
abstract: Meiotic recombination is carried out through a specialized pathway for the formation and repair of DNA double-strand breaks (DSBs) made by the Spo11 protein. The present study shed light on the functional role of cyclin, CYC2, in Tetrahymena thermophila which has transcriptionally high expression level during meiosis process. Knocking out the CYC2 gene results in arrest of meiotic conjugation process at 2.5-3.5 h after conjugation initiation, before the meiosis division starts, and in company with the absence of DSBs. To investigate the underlying mechanism of this phenomenon, a complete transcriptome profile was performed between wild-type strain and CYC2 knock-out strain. Functional analysis of RNA-Seq results identifies related differentially expressed genes (DEGs) including SPO11 and these DEGs are enriched in DNA repair/mismatch repair (MMR) terms in homologous recombination (HR), which indicates that CYC2 could play a crucial role in meiosis by regulating SPO11 and participating in HR.
authors.affiliation: Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, 230026, China.|CAS Center for Excellence in Molecular Cell Science, Shanghai, 200031, China.|School of Life Sciences, University of Science and Technology of China, Hefei, 230071, China.
authors.forename: Qianlan
authors.initials: Q
authors.lastname: Xu
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, 230071, China.
authors.forename: Ruoyu
authors.initials: R
authors.lastname: Wang
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, 230071, China.|Anatomy and Embryology Department, Suez Canal University, Ismailia, 41522, Egypt.
authors.forename: A R
authors.initials: AR
authors.lastname: Ghanam
authors.affiliation: Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Guanxiong
authors.initials: G
authors.lastname: Yan
authors.affiliation: Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Miao
authors.affiliation: Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, 230026, China. songxy5@ustc.edu.cn.|CAS Center for Excellence in Molecular Cell Science, Shanghai, 200031, China. songxy5@ustc.edu.cn.|School of Life Sciences, University of Science and Technology of China, Hefei, 230071, China. songxy5@ustc.edu.cn.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Song
mesh_terms: D059447:Cell Cycle Checkpoints; D016213:Cyclins / Q000235:genetics* / Q000378:metabolism; D053903:DNA Breaks, Double-Stranded; D053843:DNA Mismatch Repair; D004260:DNA Repair; D004706:Endodeoxyribonucleases / Q000235:genetics / Q000378:metabolism; D059765:Homologous Recombination; D008540:Meiosis; D008856:Microscopy, Fluorescence; D010641:Phenotype; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D016808:Tetrahymena thermophila / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D016213:Cyclins; D015800:Protozoan Proteins; D004706:Endodeoxyribonucleases
keywords: RNA-Seq; Tetrahymena thermophila; cyclin; homologous recombination; meiosis