title: Vascular Influence on Ventral Telencephalic Progenitors and Neocortical Interneuron Production.
abstract: The neocortex contains glutamatergic excitatory neurons and γ-aminobutyric acid (GABA)ergic inhibitory interneurons. Extensive studies have revealed substantial insights into excitatory neuron production. However, our knowledge of the generation of GABAergic interneurons remains limited. Here we show that periventricular blood vessels selectively influence neocortical interneuron progenitor behavior and neurogenesis. Distinct from those in the dorsal telencephalon, radial glial progenitors (RGPs) in the ventral telencephalon responsible for producing neocortical interneurons progressively grow radial glial fibers anchored to periventricular vessels. This progenitor-vessel association is robust and actively maintained as RGPs undergo interkinetic nuclear migration and divide at the ventricular zone surface. Disruption of this association by selective removal of INTEGRIN β1 in RGPs leads to a decrease in progenitor division, a loss of PARVALBUMIN and SOMATOSTATIN-expressing interneurons, and defective synaptic inhibition in the neocortex. These results highlight a prominent interaction between RGPs and periventricular vessels important for proper production and function of neocortical interneurons.
authors.affiliation: Developmental Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA; Biochemistry & Structural Biology, Cell & Developmental Biology, Molecular Biology Graduate Program, Weill Cornell Medical College, 1300 York Avenue, New York, NY 10065, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Tan
authors.affiliation: Developmental Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA; Biochemistry & Structural Biology, Cell & Developmental Biology, Molecular Biology Graduate Program, Weill Cornell Medical College, 1300 York Avenue, New York, NY 10065, USA.
authors.forename: Wenying Angela
authors.initials: WA
authors.lastname: Liu
authors.affiliation: Developmental Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA.
authors.forename: Xin-Jun
authors.initials: XJ
authors.lastname: Zhang
authors.affiliation: Developmental Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA; Neuroscience Graduate Program, Weill Cornell Medical College, 1300 York Avenue, New York, NY 10065, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.affiliation: Developmental Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA.
authors.forename: Si-Qiang
authors.initials: SQ
authors.lastname: Ren
authors.affiliation: Developmental Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA.
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Li
authors.affiliation: Developmental Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA.
authors.forename: Keith N
authors.initials: KN
authors.lastname: Brown
authors.affiliation: Developmental Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA; Biochemistry & Structural Biology, Cell & Developmental Biology, Molecular Biology Graduate Program, Weill Cornell Medical College, 1300 York Avenue, New York, NY 10065, USA; Neuroscience Graduate Program, Weill Cornell Medical College, 1300 York Avenue, New York, NY 10065, USA. Electronic address: shis@mskcc.org.
authors.forename: Song-Hai
authors.initials: SH
authors.lastname: Shi
mesh_terms: D000818:Animals; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D005260:Female; D005865:Gestational Age; D049452:Green Fluorescent Proteins / Q000378:metabolism; D019012:Integrin beta1 / Q000378:metabolism; D007395:Interneurons / Q000166:cytology* / Q000378:metabolism; D008473:Median Eminence / Q000098:blood supply / Q000196:embryology / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D019579:Neocortex / Q000098:blood supply* / Q000196:embryology* / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism; D010320:Parvalbumins / Q000378:metabolism; D011247:Pregnancy; D011301:Preoptic Area / Q000098:blood supply / Q000196:embryology / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D013004:Somatostatin / Q000378:metabolism; D013687:Telencephalon / Q000098:blood supply* / Q000196:embryology* / Q000378:metabolism
chemical_list: D019012:Integrin beta1; D010320:Parvalbumins; D011994:Recombinant Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D013004:Somatostatin