title: Exemestane encapsulated vitamin E-TPGS-polymeric nanoparticles: preparation, optimization, characterization, and in vitro cytotoxicity assessment.
abstract: The objective of the present study was to formulate and optimize exemestane (EXM) encapsulated vitamin E-TPGS-poly-ɛ-caprolactone based polymeric nanoparticles (TPGS-PCLNPs) for enhancing its anticancer efficacy. The 23factorial design was employed to investigate the combined influence of formulation and process variables on properties of TPGS-PCLNPs, prepared by nanoprecipitation method. The physicochemical characterization revealed the encapsulation of EXM in amorphous form without any interaction inside spherical shaped TPGS-PCLNPs. In vitro cytotoxicity study on MCF-7 breast cancer cells exhibited enhanced cytotoxicity of EXM by encapsulating in TPGS-PCLNPs. The TPGS-PCLNPs can serve as potential delivery vehicle for EXM to improve its therapeutic efficacy.
authors.affiliation: a Department of Pharmaceutics , Indian Institute of Technology (Banaras Hindu University ), Varanasi , India.
authors.forename: Brahmeshwar
authors.initials: B
authors.lastname: Mishra
authors.affiliation: a Department of Pharmaceutics , Indian Institute of Technology (Banaras Hindu University ), Varanasi , India.
authors.forename: Ravi
authors.initials: R
authors.lastname: Padaliya
authors.affiliation: a Department of Pharmaceutics , Indian Institute of Technology (Banaras Hindu University ), Varanasi , India.
authors.forename: Ravi R
authors.initials: RR
authors.lastname: Patel
mesh_terms: D000730:Androstadienes / Q000737:chemistry / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D001692:Biological Transport; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers; D004339:Drug Compounding; D065546:Drug Liberation; D005163:Factor Analysis, Statistical; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D061986:MCF-7 Cells; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry* / Q000378:metabolism; D014810:Vitamin E / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000730:Androstadienes; D000972:Antineoplastic Agents, Phytogenic; D004337:Drug Carriers; D011091:Polyesters; D014810:Vitamin E; C016240:polycaprolactone; C056516:exemestane; C014225:tocophersolan
keywords: Cytotoxicity; exemestane; factorial design; nanoprecipitation; poly-ɛ-caprolactone; vitamin E-TPGS