title: Comparative Transcriptome Analysis of Two Ascophyllum nodosum Extract Biostimulants: Same Seaweed but Different.
abstract: Biostimulants for crop management are gaining increased attention with continued demand for increased crop yields. Seaweed extracts represent one category of biostimulant, with Ascophyllum nodosum extracts (ANE) widely used for yield and quality enhancement. This study investigated how the composition of two ANE biostimulants (ANE A and ANE B) affects plant mRNA transcriptomes, using the model plant Arabidopsis thaliana. Using Affymetrix Ath1 microarrays, significant heterogeneity was detected between the ANE biostimulants in terms of their impacts on the mRNA transcriptome of A. thaliana plants, which accumulated significantly more biomass than untreated controls. Genes dysregulated by the ANE biostimulants are associated with a wide array of predicted biological processes, molecular functions, and subcellular distributions. ANE A dysregulated 4.47% of the transcriptome, whereas ANE B dysregulated 0.87%. The compositions of both ANEs were significantly different, with a 4-fold difference in polyphenol levels, the largest observed. The standardization of the composition of ANE biostimulants represents a challenge for providing consistent effects on plant gene expression and biostimulation.
authors.affiliation: Plant Biostimulant Group, Shannon Applied Biotechnology Centre, Institute of Technology , Tralee, Co. Kerry, Ireland.
authors.forename: Oscar
authors.initials: O
authors.lastname: Goñi
authors.affiliation: Genetics and Biotechnology Laboratory, Plant and AgriBiosciences Research Centre (PABC), National University of Ireland Galway , University Road, Galway H91 REW4, Ireland.
authors.forename: Antoine
authors.initials: A
authors.lastname: Fort
authors.affiliation: Plant Biostimulant Group, Shannon Applied Biotechnology Centre, Institute of Technology , Tralee, Co. Kerry, Ireland.
authors.forename: Patrick
authors.initials: P
authors.lastname: Quille
authors.affiliation: Genetics and Biotechnology Laboratory, Plant and AgriBiosciences Research Centre (PABC), National University of Ireland Galway , University Road, Galway H91 REW4, Ireland.
authors.forename: Peter C
authors.initials: PC
authors.lastname: McKeown
authors.affiliation: Genetics and Biotechnology Laboratory, Plant and AgriBiosciences Research Centre (PABC), National University of Ireland Galway , University Road, Galway H91 REW4, Ireland.
authors.forename: Charles
authors.initials: C
authors.lastname: Spillane
authors.affiliation: Plant Biostimulant Group, Shannon Applied Biotechnology Centre, Institute of Technology , Tralee, Co. Kerry, Ireland.
authors.forename: Shane
authors.initials: S
authors.lastname: O'Connell
mesh_terms: D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D044646:Ascophyllum / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D012624:Seaweed / Q000737:chemistry*; D059467:Transcriptome / Q000187:drug effects
chemical_list: D029681:Arabidopsis Proteins; D010936:Plant Extracts; D010937:Plant Growth Regulators
keywords: Arabidopsis thaliana; Ascophyllum nodosum extract; biostimulant; microarray; seaweed extract