title: Next generation sequencing for preimplantation genetic testing of blastocysts aneuploidies in women of different ages.
abstract: Most of the current preimplantation genetic screening of aneuploidies tests are based on the low quality and low density comparative genomic hybridization arrays. The results are based on fewer than 2,700 probes. Our main outcome was the association of aneuploidy rates and the women's age. Between August-December 2013, 198 blastocysts from women (mean age 36.3+-4.6) undergoing in vitro fertilization underwent routine trophectoderm biopsy. NGS was performed on Ion Torrent PGM (Life Technologies). The results were analyzed in five age groups (<31, 31-35, 36-38, 39-40 and >40). 85 blastocysts were normal according to NGS results. The results in the investigated groups were (% of normal blastocyst in each group): <31 (41.9%), 31-35 (47.6%), 36-38 (47.8%), 39-40 (37.7%) and >40 (38.5%). Our study suggests that NGS PGD is applicable for routine preimplantation genetic testing. It allows also for easy customization of the procedure for each individual patient making personalized diagnostics a reality.
authors.affiliation: Invicta Fertility and Reproductive Center, Gdansk, Warsaw, Wroclaw, Poland; Department of Obstetrics and Gynecological Nursing, Faculty of Health Sciences, Medical University of Gdansk, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Lukaszuk
authors.affiliation: Invicta Fertility and Reproductive Center, Gdansk, Warsaw, Wroclaw, Poland; Department of Obstetrics and Gynaecology, Centre of Postgraduate Medical Education, Warsaw, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Jakiel
authors.affiliation: Department of Gynecology and Oncological Gynecology, Medical University of Bialystok, Poland; Centre for Reproductive Medicine KRIOBANK, Bialystok, Poland.
authors.forename: Waldemar
authors.initials: W
authors.lastname: Kuczynski
authors.affiliation: Invicta Fertility and Reproductive Center, Gdansk, Warsaw, Wroclaw, Poland.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Pukszta
authors.affiliation: Invicta Fertility and Reproductive Center, Gdansk, Warsaw, Wroclaw, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Liss
authors.affiliation: Invicta Fertility and Reproductive Center, Gdansk, Warsaw, Wroclaw, Poland.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Plociennik
authors.affiliation: Invicta Fertility and Reproductive Center, Gdansk, Warsaw, Wroclaw, Poland.
authors.forename: Aron
authors.initials: A
authors.lastname: Lukaszuk
authors.affiliation: Invicta Fertility and Reproductive Center, Gdansk, Warsaw, Wroclaw, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Pastuszek
mesh_terms: D000328:Adult; D000782:Aneuploidy; D001755:Blastocyst / Q000166:cytology*; D005260:Female; D005307:Fertilization in Vitro; D005820:Genetic Testing; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation; D006801:Humans; D019836:Preimplantation Diagnosis / Q000295:instrumentation / Q000379:methods*; D055815:Young Adult