title: PROTECTIVE EFFECTS OF POTASSIUM TRANSPORT IN MITOCHONDRIA FROM RAT MYOMETRIUM UNDER ACTIVATION OF MITOCHONDRIAL PERMEABILITY TRANSITION PORE.
abstract: We demonstrated using PBFI K(+)-sensitive fluorescent probe an enhancement of both components of K(+)-cycle--the ATP-sensitive K(+)-uptake and quinine-sensitive K+/H(+)-exchange--under the Ca2+ induced opening of mitochondrial permeability transition pore (MPTP) in rat myometrium mitochondria. Addition of CaCl2 (100 μM to K(+)-free medium results in the enhancement of reactive oxygen species (ROS) production, which was eliminated by cyclosporine A. Addition of CaCl2 to K(+)-rich medium did not increase the rate of ROS production, but blocking of mitoK+(ATP)-channels with glybenclamide (10 mcM increased production of ROS. We conclude that K(+)-cycle exerts a protective influence in mitochondria from rat myometrium by regulation of matrix volume and rate of ROS production under the condition of Ca(2+)-induced MPTP.
authors.forename: O B
authors.initials: OB
authors.lastname: Vadzyuk
mesh_terms: D000818:Animals; D002122:Calcium Chloride / Q000378:metabolism / Q000494:pharmacology; D016572:Cyclosporine / Q000494:pharmacology; D005260:Female; D005905:Glyburide / Q000494:pharmacology; D017136:Ion Transport / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D033681:Mitochondrial Membrane Transport Proteins / Q000819:agonists* / Q000037:antagonists & inhibitors / Q000378:metabolism; D000083162:Mitochondrial Permeability Transition Pore; D009215:Myometrium / Q000737:chemistry / Q000378:metabolism; D015221:Potassium Channels / Q000378:metabolism*; D027961:Potassium-Hydrogen Antiporters / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011803:Quinine / Q000494:pharmacology; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D015221:Potassium Channels; D027961:Potassium-Hydrogen Antiporters; D017382:Reactive Oxygen Species; C117780:mitochondrial K(ATP) channel; D016572:Cyclosporine; D011803:Quinine; D002122:Calcium Chloride; D005905:Glyburide