title: A potential fluorescent probe: Maillard reaction product from glutathione and ascorbic acid for rapid and label-free dual detection of Hg(2+) and biothiols.
abstract: Maillard reactions and their fluorescent products have drawn much attention in the fields of food and life science, however, the application of fluorescent products separated from the reaction as an indicator for detection of certain substances in sensor field has not been mentioned. In this article, we report on an easy-to-synthesize and water-soluble fluorescent probe separated from the typical Maillard reaction products of glutathione and ascorbic acid, with excellent stability and high quantum yield (18.2%). The further application of the probe has been explored for dual detection of Hg(2+) and biothiols including cysteine, homocysteine, and glutathione, which is based on Hg(2+)-induced fluorescence quenching of the Maillard reaction fluorescent products (MRFPs) and the fluorescence recovery as the introduction of biothiols. This sensing system exhibits a good selectivity and sensitivity, and the linear ranges for Hg(2+), cysteine, homocysteine, and glutathione are 0.05-12, 0.5-10, 0.3-20, and 0.3-20μM, respectively. The detection limits for Hg(2+), cysteine, homocysteine, and glutathione are 22, 47, 96, and 30nM at a signal-to-noise ratio of 3, respectively. Furthermore, the practical applications of this sensor for Hg(2+) and biothiols determination in water samples and human plasma sample have been demonstrated with satisfactory results.
authors.affiliation: Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.
authors.forename: Jiang Xue
authors.initials: JX
authors.lastname: Dong
authors.affiliation: Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.
authors.forename: Xiao Fang
authors.initials: XF
authors.lastname: Song
authors.affiliation: Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.
authors.forename: Zhong Feng
authors.initials: ZF
authors.lastname: Gao
authors.affiliation: Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.
authors.forename: Bang Lin
authors.initials: BL
authors.lastname: Li
authors.affiliation: Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China. Electronic address: linb@swu.edu.cn.
authors.forename: Nian Bing
authors.initials: NB
authors.lastname: Li
authors.affiliation: Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China. Electronic address: luohq@swu.edu.cn.
authors.forename: Hong Qun
authors.initials: HQ
authors.lastname: Luo
mesh_terms: D001205:Ascorbic Acid / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D003545:Cysteine / Q000032:analysis* / Q000097:blood; D005456:Fluorescent Dyes / Q000737:chemistry*; D005978:Glutathione / Q000032:analysis* / Q000097:blood; D006710:Homocysteine / Q000032:analysis* / Q000097:blood; D006801:Humans; D057230:Limit of Detection; D015416:Maillard Reaction; D008628:Mercury / Q000032:analysis* / Q000097:blood; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014867:Water / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D014874:Water Pollutants, Chemical; D014867:Water; D006710:Homocysteine; D008628:Mercury; D005978:Glutathione; D003545:Cysteine; D001205:Ascorbic Acid
keywords: Ascorbic acid; Biothiols; Fluorescence sensor; Glutathione; Hg(2+); Maillard reaction