title: Fabrication and characterization of aligned macroporous monolith for high-performance protein chromatography.
abstract: In the present study, a freeze casting method combined with particle accumulation was applied to fabricate the aligned macroporous monolith for high-performance protein chromatography. For the preparation, the reactive colloids were first prepared by using glycidyl methacrylate and ethylene glycol dimethacrylate as monomers. Subsequently, these colloids accumulated regularly and polymerized into the aligned macroporous monolith. The aligned porous structure of the monolith was characterized by SEM, mercury intrusion, and flow hydrodynamics. The results revealed that the generated monolith was possessed of aligned macropores in size of about 10 μm and high column permeability. Finally, after being modified with sulfonated groups, the monolith was evaluated for its chromatographic performance. It demonstrated that the aligned macropores endowed the monolith with excellent adsorption capacity and high column efficiency.
authors.affiliation: Department of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, PR China. Electronic address: kfdu@scu.edu.cn.
authors.forename: Kaifeng
authors.initials: K
authors.lastname: Du
authors.affiliation: Department of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, PR China.
authors.forename: Shunmin
authors.initials: S
authors.lastname: Dan
authors.affiliation: Department of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, PR China.
authors.forename: Yongkui
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, PR China.
authors.forename: Dezhi
authors.initials: D
authors.lastname: Chai
mesh_terms: D000327:Adsorption; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004852:Epoxy Compounds / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry; D010539:Permeability; D058105:Polymerization; D016062:Porosity; D011506:Proteins / Q000032:analysis / Q000737:chemistry*
chemical_list: D004852:Epoxy Compounds; D008689:Methacrylates; D011506:Proteins; C004919:ethylene dimethacrylate; C007870:glycidyl methacrylate
keywords: Aligned macropores; Chromatography; Freeze casting method; Monolith