title: Sulfide-oxidizing bacteria establishment in an innovative microaerobic reactor with an internal silicone membrane for sulfur recovery from wastewater.
abstract: A novel bioreactor, employing a silicone membrane for microaeration, was studied for partial sulfide oxidation to elemental sulfur. The objective of this study was to assess the feasibility of using an internal silicone membrane reactor (ISMR) to treat dissolved sulfide and to characterize its microbial community. The ISMR is an effective system to eliminate sulfide produced in anaerobic reactors. Sulfide removal efficiencies reached 96 % in a combined anaerobic/microaerobic reactor and significant sulfate production did not occur. The oxygen transfer was strongly influenced by air pressure and flow. Pyrosequencing analysis indicated various sulfide-oxidizing bacteria (SOB) affiliated to the species Acidithiobacillus thiooxidans, Sulfuricurvum kujiense and Pseudomonas stutzeri attached to the membrane and also indicated similarity between the biomass deposited on the membrane wall and the biomass drawn from the material support, supported the establishment of SOB in an anaerobic sludge under microaerobic conditions. Furthermore, these results showed that the reactor configuration can develop SOB under microaerobic conditions and can improve and reestablish the sulfide conversion to elemental sulfur.
authors.affiliation: Department of Chemical Engineering, Universidad de La Frontera, Avenida Francisco Salazar, 01145, Temuco, Chile.
authors.forename: F
authors.initials: F
authors.lastname: Valdés
authors.affiliation: Biological Processes Laboratory, São Carlos School of Engineering (EESC), Center for Research, Development and Innovation in Environmental Engineering, Universidade de São Paulo (USP), Engenharia Ambiental - Bloco 4-F, Avenida João Dagnone, 1100 - Santa Angelina, 13.563-120, São Carlos, SP, Brazil. prcamiloti@gmail.com.
authors.forename: P R
authors.initials: PR
authors.lastname: Camiloti
authors.affiliation: Science and Technology Institute, Universidade Federal de Alfenas, Rodovia José Aurélio Vilela, 11999, Poços de Caldas, MG, Brazil.
authors.forename: R P
authors.initials: RP
authors.lastname: Rodriguez
authors.affiliation: Biological Processes Laboratory, São Carlos School of Engineering (EESC), Center for Research, Development and Innovation in Environmental Engineering, Universidade de São Paulo (USP), Engenharia Ambiental - Bloco 4-F, Avenida João Dagnone, 1100 - Santa Angelina, 13.563-120, São Carlos, SP, Brazil.
authors.forename: T P
authors.initials: TP
authors.lastname: Delforno
authors.affiliation: Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José, 2055, San Luis Potosí, Mexico.
authors.forename: J
authors.initials: J
authors.lastname: Carrillo-Reyes
authors.affiliation: Biological Processes Laboratory, São Carlos School of Engineering (EESC), Center for Research, Development and Innovation in Environmental Engineering, Universidade de São Paulo (USP), Engenharia Ambiental - Bloco 4-F, Avenida João Dagnone, 1100 - Santa Angelina, 13.563-120, São Carlos, SP, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Zaiat
authors.affiliation: Department of Chemical Engineering, Universidad de La Frontera, Avenida Francisco Salazar, 01145, Temuco, Chile.
authors.forename: D
authors.initials: D
authors.lastname: Jeison
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology; D010084:Oxidation-Reduction; D012828:Silicones; D013440:Sulfides / Q000378:metabolism*; D013455:Sulfur / Q000302:isolation & purification*; D062065:Wastewater / Q000032:analysis*; D018508:Water Purification / Q000379:methods*
chemical_list: D012828:Silicones; D013440:Sulfides; D013455:Sulfur; D062065:Wastewater
keywords: Elemental sulfur; Microaeration; Silicone membrane; Sulfide oxidation; Sulfide-oxidizing bacteria