title: Apple latent spherical virus vector-induced flowering for shortening the juvenile phase in Japanese gentian and lisianthus plants.
abstract: Infection by apple latent spherical virus (ALSV) vectors that promote the expression of Arabidopsis thaliana FLOWERING LOCUS T ( AtFT ) or Gentiana triflora GtFT s accelerates flowering in gentian and lisianthus plants. Apple latent spherical virus (ALSV) has isometric virus particles (25 nm in diameter) that contain two ssRNA species (RNA1 and RNA2) and three capsid proteins (Vp25, Vp20, and Vp24). ALSV vectors are used for foreign gene expression and virus-induced gene silencing in a broad range of plant species. Here, we report the infection by ALSV vectors that express FLOWERING LOCUS T (AtFT) from Arabidopsis thaliana or its homolog GtFT1 from Gentiana triflora in three gentian cultivars ('Iwate Yume Aoi' [early flowering], 'Iwate' [medium flowering], and 'Alta' [late flowering]), and two lisianthus cultivars ('Newlination Pink ver. 2' and 'Torukogikyou daburu mikkusu') promotes flowering within 90 days post-inoculation using particle bombardment. Additionally, seedlings from the progeny of the early-flowering plants were tested by tissue blot hybridization, and the results showed that ALSV was not transmitted to the next generation. The promotion of flowering in the family Gentianaceae by ALSV vectors shortened the juvenile phase from 1-3 years to 3-5 months, and thus, it could be considered as a new plant breeding technique in ornamental gentian and lisianthus plants.
authors.affiliation: Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Morioka, 020-8550, Japan.
authors.forename: Rym
authors.initials: R
authors.lastname: Fekih
authors.affiliation: Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Morioka, 020-8550, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Yamagishi
authors.affiliation: Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Morioka, 020-8550, Japan. yoshikawa@iwate-u.ac.jp.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Yoshikawa
mesh_terms: D029681:Arabidopsis Proteins / Q000235:genetics; D035264:Flowers / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors / Q000235:genetics; D030012:Gentiana / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D029965:Gentianaceae / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D008018:Life Cycle Stages / Q000235:genetics*; D027845:Malus / Q000821:virology; D000069600:Plant Breeding / Q000379:methods; D010940:Plant Proteins / Q000235:genetics; D010942:Plant Viruses / Q000235:genetics*; D030821:Plants, Genetically Modified; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity; D013997:Time Factors; D014162:Transfection / Q000379:methods
chemical_list: D029681:Arabidopsis Proteins; C418905:FT protein, Arabidopsis; D010940:Plant Proteins
keywords: ALSV vector; FLOWERING LOCUS T; Floral promotion; Gentianaceae; New plant breeding technique; TERMINAL FLOWER 1