title: Anti-B7-H3 monoclonal antibody ameliorates the damage of acute experimental pancreatitis by attenuating the inflammatory response.
abstract: B7-H3, a recently discovered B7 family member, is documented as a regulator in the inflammatory response as well as T cell-mediated immune responses. In this paper, we find that patients with acute pancreatitis revealed overwhelming levels of serum soluble B7-H3 (sB7-H3) associated with the clinical outcomes. Furthermore, B7-H3 protein was marked increased in l-arginine-induced acute experimental pancreatitis. Anti-B7-H3 monoclonal antibody treatment attenuated the proinflammatory cytokine production, downregulated the activation of the NF-κB signaling pathway, and ameliorated the pancreas disruption in l-arginine-induced pancreatitis. In addition, although l-arginine alone failed to induce the production of proinflammatory cytokine and anti-B7-H3 mAb had no effect on the proinflammatory cytokine production of acinar cells, administration of anti-B7-H3 mAb in the coculture model of acinar cells and macrophages stimulated by l-arginine displayed the similar effects. On the whole, B7-H3 participates in the development of acute pancreatitis, and anti-B7-H3 monoclonal antibody ameliorates severity of acute experimental pancreatitis via attenuation of the inflammatory response.
authors.affiliation: Department of Gastroenterology and Digestive Diseases, the First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou 215006, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zhuang
authors.affiliation: Department of Gastroenterology and Digestive Diseases, the First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou 215006, China.
authors.forename: Jiaqing
authors.initials: J
authors.lastname: Shen
authors.affiliation: Department of Gastroenterology and Digestive Diseases, the First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou 215006, China.
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Jia
authors.affiliation: Department of Gastroenterology and Digestive Diseases, the First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou 215006, China.
authors.forename: Airong
authors.initials: A
authors.lastname: Wu
authors.affiliation: Department of Gastroenterology and Digestive Diseases, the First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou 215006, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Xu
authors.affiliation: Department of Gastroenterology and Digestive Diseases, the First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou 215006, China.
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Gastroenterology and Digestive Diseases, the First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou 215006, China. Electronic address: xcf150709@163.com.
authors.forename: Chunfang
authors.initials: C
authors.lastname: Xu
mesh_terms: D061354:Acinar Cells / Q000187:drug effects* / Q000502:physiology; D000208:Acute Disease; D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D001120:Arginine / Q000633:toxicity; D060887:B7 Antigens / Q000276:immunology*; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D006801:Humans; D007167:Immunotherapy / Q000379:methods*; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology / Q000628:therapy*; D018836:Inflammation Mediators / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000502:physiology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D016328:NF-kappa B / Q000378:metabolism; D010195:Pancreatitis / Q000139:chemically induced / Q000276:immunology / Q000628:therapy*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000911:Antibodies, Monoclonal; D060887:B7 Antigens; C423237:CD276 protein, human; D016207:Cytokines; D018836:Inflammation Mediators; D016328:NF-kappa B; D001120:Arginine
keywords: Acute pancreatitis; B7-H3; Inflammation; NF-κB