title: Anti-bacterial and anti-inflammatory effects of ethanol extract from Houttuynia cordata poultice.
abstract: Houttuynia cordata (HC) has been commonly used as many traditional remedies in local areas of Japan. Although many pharmacological activities of HC have been reported, the mechanism underlying the effect of HC remains unknown. We conducted the interview survey in Japan to verify how HC was actually used. The interview survey revealed that HC poultice (HCP) prepared from smothering fresh leaves of HC was most frequently used for the treatment of purulent skin diseases including furuncle and carbuncle with high effectiveness. Ethanol extract of HCP (eHCP) showed anti-bacterial effects against methicillin-resistant Staphylococcus aureus (MRSA), and showed an anti-biofilm activity against MRSA. eHCP showed dose-dependent inhibition of S. aureus lipoteichoic acid (LTA)-induced interleukin-8 and CCL20 production in human keratinocyte without any cytotoxicity. These results suggest that HCP is effective for skin abscess and its underlying mechanism might be the complicated multiple activities for both bacteria and host cells.
authors.affiliation: a Department of Pharmacognosy , Institute of Biomedical Sciences, Tokushima University Graduate School , Tokushima , Japan.
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Sekita
authors.affiliation: b Department of Oral Microbiology, Institute of Biomedical Sciences , Tokushima University Graduate School , Tokushima , Japan.
authors.forename: Keiji
authors.initials: K
authors.lastname: Murakami
authors.affiliation: c Department of Conservative Dentistry, Institute of Biomedical Sciences , Tokushima University Graduate School , Tokushima , Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Yumoto
authors.affiliation: d Department of Molecular Pharmacology, Institute of Biomedical Sciences , Tokushima University Graduate School , Tokushima , Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mizuguchi
authors.affiliation: b Department of Oral Microbiology, Institute of Biomedical Sciences , Tokushima University Graduate School , Tokushima , Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Amoh
authors.affiliation: e Faculty of Medicine, Graduate Schools of Allied Health Sciences , Osaka University , Osaka , Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ogino
authors.affiliation: c Department of Conservative Dentistry, Institute of Biomedical Sciences , Tokushima University Graduate School , Tokushima , Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Matsuo
authors.affiliation: b Department of Oral Microbiology, Institute of Biomedical Sciences , Tokushima University Graduate School , Tokushima , Japan.
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Miyake
authors.affiliation: f Department of Molecular Studies for Incurable Diseases, Institute of Biomedical Sciences , Tokushima University Graduate School , Tokushima , Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Fukui
authors.affiliation: a Department of Pharmacognosy , Institute of Biomedical Sciences, Tokushima University Graduate School , Tokushima , Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Kashiwada
mesh_terms: D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D002270:Carbuncle / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D002461:Cell Line, Transformed; D054418:Chemokine CCL20 / Q000096:biosynthesis / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D000431:Ethanol; D005260:Female; D005667:Furunculosis / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D052859:Houttuynia / Q000737:chemistry*; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis / Q000378:metabolism; D007564:Japan; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D008517:Phytotherapy / Q000706:statistics & numerical data*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D013203:Staphylococcal Infections / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D011795:Surveys and Questionnaires; D013682:Teichoic Acids / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; C108355:CCL20 protein, human; D054418:Chemokine CCL20; D016209:Interleukin-8; D008070:Lipopolysaccharides; D010936:Plant Extracts; D013682:Teichoic Acids; D000431:Ethanol; C009900:lipoteichoic acid
keywords: CCL20; Houttuynia cordata poultice; anti-biofilm activity; interleukin-8; methicillin-resistant Staphylococcus aureus