title: Placenta-Enriched LincRNAs MIR503HG and LINC00629 Decrease Migration and Invasion Potential of JEG-3 Cell Line.
abstract: LINC00629 and MIR503HG are long intergenic non-coding RNAs (lincRNAs) mapped on chromosome X (Xq26), a region enriched for genes associated with human reproduction. Genes highly expressed in normal reproductive tissues and cancers (CT genes) are well known as potential tumor biomarkers. This study aimed to characterize the structure, expression, function and regulation mechanism of MIR503HG and LINC00629 lincRNAs. According to our data, MIR503HG expression was almost exclusive to placenta and LINC00629 was highly expressed in placenta and other reproductive tissues. Further analysis, using a cancer cell lines panel, showed that MIR503HG and LINC00629 were expressed in 50% and 100% of the cancer cell lines, respectively. MIR503HG was expressed predominantly in the nucleus of JEG-3 choriocarcinoma cells. We observed a positively correlated expression between MIR503HG and LINC00629, and between the lincRNAs and neighboring miRNAs. Also, both LINC00629 and MIR503GH could be negatively regulated by DNA methylation in an indirect way. Additionally, we identified new transcripts for MIR503HG and LINC00629 that are relatively conserved when compared to other primates. Furthermore, we found that overexpression of MIR503HG2 and the three-exon LINC00629 new isoforms decreased invasion and migration potential of JEG-3 tumor cell line. In conclusion, our results suggest that lincRNAs MIR503HG and LINC00629 impaired migration and invasion capacities in a choriocarcinoma in vitro model, indicating a potential role in human reproduction and tumorigenesis. Moreover, the MIR503HG expression pattern found here could indicate a putative new tumor biomarker.
authors.affiliation: Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.|Center for Cell-Based Therapy (CEPID/FAPESP), National institute of Science and Technology in Stem Cell and Cell Therapy (INCTC/CNPq), Regional Blood Center of Ribeirão Preto, Riberão Preto, Brazil.|Center for Medical Genomics (HCFMRP/USP), Center for Integrative Systems Biology (CISBi-NAP/USP), Ribeirão Preto, Brazil.
authors.forename: Bruna Rodrigues
authors.initials: BR
authors.lastname: Muys
authors.affiliation: Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.|Center for Cell-Based Therapy (CEPID/FAPESP), National institute of Science and Technology in Stem Cell and Cell Therapy (INCTC/CNPq), Regional Blood Center of Ribeirão Preto, Riberão Preto, Brazil.|Center for Medical Genomics (HCFMRP/USP), Center for Integrative Systems Biology (CISBi-NAP/USP), Ribeirão Preto, Brazil.
authors.forename: Júlio Cesar Cetrulo
authors.initials: JC
authors.lastname: Lorenzi
authors.affiliation: Gonçalo Moniz Research Center, Oswaldo Cruz Foundation, Bahia, Brazil.
authors.forename: Dalila Luciola
authors.initials: DL
authors.lastname: Zanette
authors.affiliation: Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.|Center for Cell-Based Therapy (CEPID/FAPESP), National institute of Science and Technology in Stem Cell and Cell Therapy (INCTC/CNPq), Regional Blood Center of Ribeirão Preto, Riberão Preto, Brazil.|Center for Medical Genomics (HCFMRP/USP), Center for Integrative Systems Biology (CISBi-NAP/USP), Ribeirão Preto, Brazil.
authors.forename: Rafaela de Barros
authors.initials: Rde B
authors.lastname: Lima e Bueno
authors.affiliation: Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.|Center for Cell-Based Therapy (CEPID/FAPESP), National institute of Science and Technology in Stem Cell and Cell Therapy (INCTC/CNPq), Regional Blood Center of Ribeirão Preto, Riberão Preto, Brazil.|Center for Medical Genomics (HCFMRP/USP), Center for Integrative Systems Biology (CISBi-NAP/USP), Ribeirão Preto, Brazil.
authors.forename: Luíza Ferreira
authors.initials: LF
authors.lastname: de Araújo
authors.affiliation: Center for Cell-Based Therapy (CEPID/FAPESP), National institute of Science and Technology in Stem Cell and Cell Therapy (INCTC/CNPq), Regional Blood Center of Ribeirão Preto, Riberão Preto, Brazil.|Center for Medical Genomics (HCFMRP/USP), Center for Integrative Systems Biology (CISBi-NAP/USP), Ribeirão Preto, Brazil.
authors.forename: Anemari Ramos
authors.initials: AR
authors.lastname: Dinarte-Santos
authors.affiliation: Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.|Center for Cell-Based Therapy (CEPID/FAPESP), National institute of Science and Technology in Stem Cell and Cell Therapy (INCTC/CNPq), Regional Blood Center of Ribeirão Preto, Riberão Preto, Brazil.|Center for Medical Genomics (HCFMRP/USP), Center for Integrative Systems Biology (CISBi-NAP/USP), Ribeirão Preto, Brazil.
authors.forename: Cleidson Pádua
authors.initials: CP
authors.lastname: Alves
authors.affiliation: Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.|Center for Cell-Based Therapy (CEPID/FAPESP), National institute of Science and Technology in Stem Cell and Cell Therapy (INCTC/CNPq), Regional Blood Center of Ribeirão Preto, Riberão Preto, Brazil.
authors.forename: Anelisa
authors.initials: A
authors.lastname: Ramão
authors.affiliation: Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.|Center for Cell-Based Therapy (CEPID/FAPESP), National institute of Science and Technology in Stem Cell and Cell Therapy (INCTC/CNPq), Regional Blood Center of Ribeirão Preto, Riberão Preto, Brazil.|Center for Medical Genomics (HCFMRP/USP), Center for Integrative Systems Biology (CISBi-NAP/USP), Ribeirão Preto, Brazil.
authors.forename: Greice Andreotti
authors.initials: GA
authors.lastname: de Molfetta
authors.affiliation: Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, Brazil.
authors.forename: Daniel Onofre
authors.initials: DO
authors.lastname: Vidal
authors.affiliation: Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.|Center for Cell-Based Therapy (CEPID/FAPESP), National institute of Science and Technology in Stem Cell and Cell Therapy (INCTC/CNPq), Regional Blood Center of Ribeirão Preto, Riberão Preto, Brazil.|Center for Medical Genomics (HCFMRP/USP), Center for Integrative Systems Biology (CISBi-NAP/USP), Ribeirão Preto, Brazil.
authors.forename: Wilson Araújo
authors.initials: WA
authors.lastname: Silva
mesh_terms: D001374:Azacitidine / Q000494:pharmacology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics*; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D017124:Conserved Sequence / Q000235:genetics; D019175:DNA Methylation / Q000235:genetics; D019143:Evolution, Molecular; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009690:Nucleic Acid Conformation; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D012098:Reproduction / Q000235:genetics
chemical_list: C545473:MIRN503 microRNA, human; D035683:MicroRNAs; D020033:Protein Isoforms; D062085:RNA, Long Noncoding; C000610379:long non-coding RNA LINC00629, human; C000610378:long non-coding RNA MIR503HG, human; D001374:Azacitidine