title: Is the Quantification of Antigen-Specific Basophil Activation a Useful Tool for Monitoring Oral Tolerance Induction in Children With Egg Allergy?
abstract: OBJECTIVE
  To assess modifications in baseline specific IgE- and anti-IgE- and antigen-specific-mediated basophil activation in egg-allergic children. The values were compared before and after the children completed specific oral tolerance induction (SOTI) with egg.
  
  
  METHODS
  We studied 28 egg-allergic children who completed SOTI with egg. The basophil activation test and specific IgE determinations with egg white, ovalbumin, and ovomucoid were performed in all 28 children.
  
  
  RESULTS
  A decrease in antigen-specific activation with egg white, ovalbumin, and ovomucoid was observed only at the 2 lowest concentrations used (5 and 0.05 ng/mL). Baseline activation was higher in patients with multiple food allergies and in those who developed anaphylaxis during SOTI; this activation decreased in both groups after completion of SOTI. A significant decrease was also observed in specific IgE values for egg white, ovalbumin, and ovomucoid after tolerance induction.
  
  
  CONCLUSIONS
  Food tolerance induction is a specific process for each food that can be mediated by immunologic changes such as a decrease in specific IgE values and in specific and spontaneous basophil activation.
authors.forename: P M
authors.initials: PM
authors.lastname: Gamboa
authors.forename: E
authors.initials: E
authors.lastname: Garcia-Lirio
authors.forename: C
authors.initials: C
authors.lastname: Gonzalez
authors.forename: A
authors.initials: A
authors.lastname: Gonzalez
authors.lastname: Martinez-Aranguren R M
authors.forename: L
authors.initials: L
authors.lastname: Sanz María
mesh_terms: D000707:Anaphylaxis / Q000097:blood / Q000175:diagnosis / Q000276:immunology / Q000628:therapy*; D000941:Antigens / Q000276:immunology*; D001491:Basophils / Q000276:immunology*; D015415:Biomarkers / Q000097:blood; D002648:Child; D002675:Child, Preschool; D003888:Desensitization, Immunologic / Q000379:methods*; D004306:Dose-Response Relationship, Immunologic; D021181:Egg Hypersensitivity / Q000097:blood / Q000175:diagnosis / Q000276:immunology / Q000628:therapy*; D004529:Egg White; D005260:Female; D006801:Humans; D007108:Immune Tolerance; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D007428:Intradermal Tests; D008297:Male; D015166:Monitoring, Immunologic; D010047:Ovalbumin / Q000276:immunology; D010059:Ovomucin / Q000276:immunology; D011237:Predictive Value of Tests; D016896:Treatment Outcome
chemical_list: D000941:Antigens; D015415:Biomarkers; D010059:Ovomucin; D007073:Immunoglobulin E; D010047:Ovalbumin