title: Effect of Liposomes with Different Double Arms Polyethyleneglycol on Hepatic Metastasis Model Mice and Evaluation Using a Fluorescent Imaging Device.
abstract: BACKGROUND
  We have previously reported the synthesis of a novel polyethyleneglycol (PEG) lipid, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-PEG (DDA-PEG). This study aimed to clarify the anti-metastatic effect and localization of DDA-PEG-modified liposomes on a murine hepatic metastasis model.
  
  
  METHODS
  M5076 ovarian sarcoma cells were inoculated for hepatic metastasis model mice. The accumulation of liposomes in the tumor and metastatic sites was detected by fluorescent imaging device. In metastasis study, doxorubicin (DOX) loaded DDA-PEG-modified liposome (DDA-LDOX) was injected. Alexa Fluor 790 NHS Ester loaded DDA-PEG-modified liposomes were used to detect fluorescence intensity at metastatic sites when visualized topically using a fluorescence imaging device.
  
  
  RESULTS
  DDA-PEG-modified liposomes accumulated at the sites of hepatic metastasis but not in the normal hepatocytes. Furthermore, the DDA-LDOX inhibited metastasis in this model. The survival time of M5076 ovarian sarcoma bearing mice in DDA-LDOX group was longer than those in control, DOX solution and the other PEG-modified liposomal DOX groups, and the survival ratio in DDALDOX group remained 66.7% until 60 days after treatment.
  
  
  CONCLUSIONS
  It is expected that the DDA-PEG-modified liposomes will extensively contribute in clinical practice as a superior drug carrier because this liposomes proved to be effective against metastasis.
authors.affiliation: School of Medicine, Iwate Medical University, 2-1-1 Nishitokuta, Yahaba-cho, Shiwa-gun, Iwate 028-3694, Japan.
authors.forename: Ikumi
authors.initials: I
authors.lastname: Sugiyama
authors.affiliation: School of Medicine, Iwate Medical University, 2-1-1 Nishitokuta, Yahaba-cho, Shiwa-gun, Iwate 028-3694, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Oikawa
authors.affiliation: School of Medicine, Iwate Medical University, 2-1-1 Nishitokuta, Yahaba-cho, Shiwa-gun, Iwate 028-3694, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Masuda
authors.affiliation: School of Medicine, Iwate Medical University, 2-1-1 Nishitokuta, Yahaba-cho, Shiwa-gun, Iwate 028-3694, Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Sadzuka
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004337:Drug Carriers; D005260:Female; D005453:Fluorescence; D005456:Fluorescent Dyes; D008055:Lipids; D008081:Liposomes; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000556:secondary; D008297:Male; D051379:Mice; D009362:Neoplasm Metastasis / Q000188:drug therapy*; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000473:pathology; D011092:Polyethylene Glycols
chemical_list: D004337:Drug Carriers; D005456:Fluorescent Dyes; D008055:Lipids; D008081:Liposomes; D011092:Polyethylene Glycols
keywords: Fluoresent imaging device; hepatic metastasis; liposome; passive targeting; polyethyleneglycol; tumor affinity