title: Comparative analysis of microRNA profiles of rice anthers between cool-sensitive and cool-tolerant cultivars under cool-temperature stress.
abstract: Plants subjected to abiotic stress can regulate gene expression post-transcriptionally by means of small RNAs such as microRNAs. Cool-temperature stress causes abnormal tapetum hypertrophy in rice anthers, leading to pollen sterility. As a first step toward understanding the molecular mechanisms of cool tolerance in developing anthers of rice, we report here a comprehensive comparative analysis of microRNAs between cool-sensitive Sasanishiki and cool-tolerant Hitomebore cultivars. High-throughput Illumina sequencing revealed 241 known and 46 novel microRNAs. Interestingly, 15 of these microRNAs accumulated differentially in the two cultivars at the uninucleate microspore stage under cool conditions. Inverse correlations between expression patterns of microRNAs and their target genes were confirmed by quantitative RT-PCR analysis, and cleavage sites of some of the target genes were determined by 5' RNA ligase-mediated RACE experiments. Thus, our data are useful resources to elucidate microRNA-mediated mechanism(s) of cool tolerance in rice anthers at the booting stage.
authors.affiliation: Graduate School of Life Sciences, Tohoku University.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Maeda
authors.forename: Satomi
authors.initials: S
authors.lastname: Sakazono
authors.forename: Hiromi
authors.initials: H
authors.lastname: Masuko-Suzuki
authors.forename: Midori
authors.initials: M
authors.lastname: Taguchi
authors.forename: Kaori
authors.initials: K
authors.lastname: Yamamura
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Nagano
authors.forename: Takashi
authors.initials: T
authors.lastname: Endo
authors.forename: Kenichi
authors.initials: K
authors.lastname: Saeki
authors.forename: Masaaki
authors.initials: M
authors.lastname: Osaka
authors.forename: Moe
authors.initials: M
authors.lastname: Nabemoto
authors.forename: Kana
authors.initials: K
authors.lastname: Ito
authors.forename: Toru
authors.initials: T
authors.lastname: Kudo
authors.forename: Masaaki
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Mizuho
authors.initials: M
authors.lastname: Kawagishi
authors.forename: Kotomi
authors.initials: K
authors.lastname: Fujita
authors.forename: Hikaru
authors.initials: H
authors.lastname: Nanjo
authors.forename: Tomoe
authors.initials: T
authors.lastname: Shindo
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yano
authors.forename: Go
authors.initials: G
authors.lastname: Suzuki
authors.forename: Keita
authors.initials: K
authors.lastname: Suwabe
authors.forename: Masao
authors.initials: M
authors.lastname: Watanabe
mesh_terms: D003080:Cold Temperature; D035264:Flowers / Q000235:genetics* / Q000254:growth & development; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D011058:Pollen / Q000235:genetics / Q000254:growth & development; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D035683:MicroRNAs