title: Optimized Formulation of a Thermostable Spray-Dried Virus-Like Particle Vaccine against Human Papillomavirus.
abstract: Existing vaccines against human papillomavirus (HPV) require continuous cold-chain storage. Previously, we developed a bacteriophage virus-like particle (VLP)-based vaccine for HPV infection, which elicits broadly neutralizing antibodies against diverse HPV types. Here, we formulated these VLPs into a thermostable dry powder using a multicomponent excipient system and by optimizing the spray-drying parameters using a half-factorial design approach. Dry-powder VLPs were stable after spray drying and after long-term storage at elevated temperatures. Immunization of mice with a single dose of reconstituted dry-powder VLPs that were stored at 37 °C for more than a year elicited high anti-L2 IgG antibody titers. Spray-dried thermostable, broadly protective L2 bacteriophage VLPs vaccine could be accessible to remote regions of the world (where ∼84% of cervical cancer patients reside) by eliminating the cold-chain requirement during transportation and storage.
authors.affiliation: Department of Industrial and Physical Pharmacy, College of Pharmacy, Purdue University , West Lafayette, Indiana 47907, United States.
authors.forename: Sugandha
authors.initials: S
authors.lastname: Saboo
authors.affiliation: Department of Biological Sciences, Michigan Technological University , Houghton, Michigan 49931, United States.
authors.forename: Ebenezer
authors.initials: E
authors.lastname: Tumban
authors.forename: Julianne
authors.initials: J
authors.lastname: Peabody
authors.affiliation: Department of Cell Biology and Molecular Genetics, University of Maryland , College Park, Maryland 20742, United States.
authors.forename: Denis
authors.initials: D
authors.lastname: Wafula
authors.forename: David S
authors.initials: DS
authors.lastname: Peabody
authors.forename: Bryce
authors.initials: B
authors.lastname: Chackerian
authors.forename: Pavan
authors.initials: P
authors.lastname: Muttil
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D002626:Chemistry, Pharmaceutical / Q000379:methods; D006801:Humans; D007114:Immunization / Q000379:methods; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D027383:Papillomaviridae / Q000276:immunology*; D030361:Papillomavirus Infections / Q000276:immunology*; D011208:Powders / Q000008:administration & dosage / Q000737:chemistry; D013696:Temperature; D014611:Vaccination / Q000379:methods; D058425:Vaccines, Virus-Like Particle / Q000737:chemistry* / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D007074:Immunoglobulin G; D011208:Powders; D058425:Vaccines, Virus-Like Particle
keywords: cold-chain; design of experiment; dry powder; spray drying; thermostable vaccine; virus-like particles