title: Identification of the Three Genes Involved in Controlling Production of a Phytotoxin Tropolone in Burkholderia plantarii.
abstract: Tropolone, a phytotoxin produced by Burkholderia plantarii, causes rice seedling blight. To identify genes involved in tropolone synthesis, we systematically constructed mutations in the genes encoding 55 histidine kinases and 72 response regulators. From the resulting defective strains, we isolated three mutants, KE1, KE2, and KE3, in which tropolone production was repressed. The deleted genes of these mutants were named troR1, troK, and troR2, respectively. The mutant strains did not cause rice seedling blight, and complementation experiments indicated that TroR1, TroK, and TroR2 were involved in the synthesis of tropolone in B. plantarii However, tropolone synthesis was repressed in the TroR1 D52A, TroK H253A, and TroR2 D46A site-directed mutants. These results suggest that the putative sensor kinase (TroK) and two response regulators (TroR1 and TroR2) control the production of tropolone in B. plantarii
  
  
  
  A two-component system is normally composed of a sensor histidine kinase (HK) and a cognate response regulator (RR) pair. In this study, HK (TroK) and two RRs (TroR1 and TroR2) were found to be involved in controlling tropolone production in B. plantarii These three genes may be part of a bacterial signal transduction network. Such networks are thought to exist in other bacteria to regulate phytotoxin production, as well as environmental adaptation and signal transduction.
authors.affiliation: Department of Bioscience, Graduate School of Agriculture, Kinki University, Nara, Japan.
authors.forename: Shunpei
authors.initials: S
authors.lastname: Miwa
authors.affiliation: Department of Bioscience, Graduate School of Agriculture, Kinki University, Nara, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Kihira
authors.affiliation: Department of Bioscience, Graduate School of Agriculture, Kinki University, Nara, Japan.
authors.forename: Akinori
authors.initials: A
authors.lastname: Yoshioka
authors.affiliation: Department of Biotechnology and Chemistry, Faculty of Engineering, Kinki University, Hiroshima, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Nakasone
authors.affiliation: Department of Bioscience, Graduate School of Agriculture, Kinki University, Nara, Japan.|Genetic Strains Research Center, National Institute of Genetics, Shizuoka, Japan.
authors.forename: Sho
authors.initials: S
authors.lastname: Okamoto
authors.affiliation: Institute of Microbial Chemistry (BIKAKEN), Tokyo, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Hatano
authors.affiliation: Institute of Microbial Chemistry (BIKAKEN), Tokyo, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Igarashi
authors.affiliation: Department of Bioscience, Graduate School of Agriculture, Kinki University, Nara, Japan.|Department of Science and Technology on Food Safety, Faculty of Biology-Oriented Science and Technology, Kinki University, Wakayama, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Eguchi
authors.affiliation: Department of Bioscience, Graduate School of Agriculture, Kinki University, Nara, Japan.
authors.forename: Akinori
authors.initials: A
authors.lastname: Kato
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation, Tokyo, Japan.
authors.forename: Natsuko
authors.initials: N
authors.lastname: Ichikawa
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation, Tokyo, Japan.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sekine
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation, Tokyo, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Fujita
authors.affiliation: NODAI Genome Research Center, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Kanesaki
authors.affiliation: NODAI Genome Research Center, Tokyo University of Agriculture, Tokyo, Japan.|Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yoshikawa
authors.affiliation: Department of Bioscience, Graduate School of Agriculture, Kinki University, Nara, Japan utsumi@nara.kindai.ac.jp.
authors.forename: Ryutaro
authors.initials: R
authors.lastname: Utsumi
mesh_terms: D019117:Burkholderia / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D015394:Molecular Structure; D012275:Oryza / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology; D014334:Tropolone / Q000737:chemistry / Q000378:metabolism*
chemical_list: D014334:Tropolone