title: Vascular imaging with contrast agent in hard and soft tissues using microcomputed-tomography.
abstract: Vascularization is essential for many tissues and is a main requisite for various tissue-engineering strategies. Different techniques are used for highlighting vasculature, in vivo and ex vivo, in 2-D or 3-D including histological staining, immunohistochemistry, radiography, angiography, microscopy, computed tomography (CT) or micro-CT, both stand-alone and synchrotron system. Vascularization can be studied with or without a contrast agent. This paper presents the results obtained with the latest Skyscan micro-CT (Skyscan 1272, Bruker, Belgium) following barium sulphate injection replacing the bloodstream in comparison with results obtained with a Skyscan In Vivo 1076. Different hard and soft tissues were perfused with contrast agent and were harvested. Samples were analysed using both forms of micro-CT, and improved results were shown using this new micro-CT. This study highlights the vasculature using micro-CT methods. The results obtained with the Skyscan 1272 are clearly defined compared to results obtained with Skyscan 1076. In particular, this instrument highlights the high number of small vessels, which were not seen before at lower resolution. This new micro-CT opens broader possibilities in detection and characterization of the 3-D vascular tree to assess vascular tissue engineering strategies.
authors.affiliation: Inserm U791, LIOAD, Faculté de Chirurgie Dentaire, 1 Place Alexis Ricordeau, 44042 Nantes Cedex 1, France.|Faculté de chirurgie dentaire, Université de Nantes, 1 Place Alexis Ricordeau, 44042 Nantes Cedex 1, France.
authors.forename: P
authors.initials: P
authors.lastname: Blery
authors.affiliation: Inserm U791, LIOAD, Faculté de Chirurgie Dentaire, 1 Place Alexis Ricordeau, 44042 Nantes Cedex 1, France.
authors.forename: P
authors.initials: P
authors.lastname: Pilet
authors.affiliation: Inserm U1059, Laboratoire de Biologie intégrative du Tissu Osseux, Faculté de Médecine, 15 rue Ambroise Paré, 42023 Saint-Etienne cedex.
authors.forename: A Vanden-
authors.initials: AV
authors.lastname: Bossche
authors.affiliation: Inserm U791, LIOAD, Faculté de Chirurgie Dentaire, 1 Place Alexis Ricordeau, 44042 Nantes Cedex 1, France.|Service d'ORL et de chirurgie cervico-faciale, CHU Hôtel Dieu, 1 place Alexis Ricordeau, 44042 Nantes Cedex 1, France.
authors.forename: A
authors.initials: A
authors.lastname: Thery
authors.affiliation: Inserm U791, LIOAD, Faculté de Chirurgie Dentaire, 1 Place Alexis Ricordeau, 44042 Nantes Cedex 1, France.
authors.forename: J
authors.initials: J
authors.lastname: Guicheux
authors.affiliation: Inserm U791, LIOAD, Faculté de Chirurgie Dentaire, 1 Place Alexis Ricordeau, 44042 Nantes Cedex 1, France.|Faculté de chirurgie dentaire, Université de Nantes, 1 Place Alexis Ricordeau, 44042 Nantes Cedex 1, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Amouriq
authors.affiliation: Inserm U791, LIOAD, Faculté de Chirurgie Dentaire, 1 Place Alexis Ricordeau, 44042 Nantes Cedex 1, France.|Service d'ORL et de chirurgie cervico-faciale, CHU Hôtel Dieu, 1 place Alexis Ricordeau, 44042 Nantes Cedex 1, France.
authors.forename: F
authors.initials: F
authors.lastname: Espitalier
authors.affiliation: IRSN Institut de Radioprotection et de Sûreté Nucléaire, IRSN/PRP-HOM/SRBE/LR2I, 31 avenue de la division Leclerc BP17, 92260 Fontenay aux roses, France.
authors.forename: N
authors.initials: N
authors.lastname: Mathieu
authors.affiliation: Inserm U791, LIOAD, Faculté de Chirurgie Dentaire, 1 Place Alexis Ricordeau, 44042 Nantes Cedex 1, France.|Faculté de chirurgie dentaire, Université de Nantes, 1 Place Alexis Ricordeau, 44042 Nantes Cedex 1, France.
authors.forename: P
authors.initials: P
authors.lastname: Weiss
mesh_terms: D000792:Angiography / Q000379:methods*; D000818:Animals; D001466:Barium Sulfate / Q000008:administration & dosage; D001808:Blood Vessels / Q000000981:diagnostic imaging*; D003287:Contrast Media / Q000008:administration & dosage*; D017207:Rats, Sprague-Dawley; D055114:X-Ray Microtomography / Q000379:methods*
chemical_list: D003287:Contrast Media; D001466:Barium Sulfate
keywords: Barium sulphate; contrast agent; imaging; micro-CT; vascularization