title: Investigation of early and advanced stages in ovarian cancer using human plasma by differential scanning calorimetry and mass spectrometry.
abstract: Ovarian cancer is recognized with high mortality due to asymptomatic nature of the disease and difficulties in diagnosing early stage of the cancer. The present study evaluates the use of differential scanning calorimetry (DSC) in differentiating the severity of ovarian cancer from healthy women. 47 diseased women were subdivided into four stages with respect to clinical relevance and severity. Stages I-II were regarded as early stages and stages III-IV were regarded as advanced stages. The two average transition temperatures (T m ) increased with disease severity from 64.84 and 70.32 °C (healthy) to 68.46 and 75.24 °C (stage IV), respectively. T m were increased depending on clinical groups. In addition, the change in heat capacity was also dependent on the disease severity. To further support and investigate the nature of the proposed interactions, matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis is employed. The results suggest the differences in peptide expression between early and advanced stage of ovarian cancer, affected abundant proteins in plasma. The combined DSC and MS approach was supportive in identifying a unique signature of ovarian cancer stages, and demonstrates the potential of DSC as a complementary diagnostic tool in the evaluation of early stage ovarian cancer.
authors.affiliation: College of Pharmacy, Dongguk University-Seoul, Goyang, Gyeonggi, 410-820, Republic of Korea.
authors.forename: Nam Ah
authors.initials: NA
authors.lastname: Kim
authors.affiliation: Department of Obstetrics and Gynecology, Ewha Womans University, College of Medicine, Seoul, 158-710, Republic of Korea.
authors.forename: Jing Hui
authors.initials: JH
authors.lastname: Jin
authors.affiliation: Research Institute, National Cancer Center, Goyang, Gyeonggi, 410-769, Republic of Korea.
authors.forename: Kyung-Hee
authors.initials: KH
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Dongguk University-Seoul, Goyang, Gyeonggi, 410-820, Republic of Korea.
authors.forename: Dae Gon
authors.initials: DG
authors.lastname: Lim
authors.affiliation: Research Institute, National Cancer Center, Goyang, Gyeonggi, 410-769, Republic of Korea.
authors.forename: Heesun
authors.initials: H
authors.lastname: Cheong
authors.affiliation: Department of Obstetrics and Gynecology, Ewha Womans University, College of Medicine, Seoul, 158-710, Republic of Korea.
authors.forename: Yun Hwan
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Department of Obstetrics and Gynecology, Ewha Womans University, College of Medicine, Seoul, 158-710, Republic of Korea.
authors.forename: Woong
authors.initials: W
authors.lastname: Ju
authors.affiliation: Department of Obstetrics and Gynecology, Ewha Womans University, College of Medicine, Seoul, 158-710, Republic of Korea.
authors.forename: Seung Cheol
authors.initials: SC
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Dongguk University-Seoul, Goyang, Gyeonggi, 410-820, Republic of Korea. shjeong@dongguk.edu.
authors.forename: Seong Hoon
authors.initials: SH
authors.lastname: Jeong
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis*; D002152:Calorimetry, Differential Scanning / Q000379:methods*; D016022:Case-Control Studies; D003936:Diagnosis, Computer-Assisted; D005260:Female; D006801:Humans; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000097:blood* / Q000473:pathology; D040081:Protein Array Analysis; D011489:Protein Denaturation; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013817:Thermography / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor; D001798:Blood Proteins; D020543:Proteome
keywords: Diagnostic tool; Differential scanning calorimetry; Early stage; Ovarian cancer; T m