title: Cost-effective imprinting combining macromolecular crowding and a dummy template for the fast purification of punicalagin from pomegranate husk extract.
abstract: The combination of molecular crowding and virtual imprinting was employed to develop a cost-effective method to prepare molecularly imprinted polymers. By using linear polymer polystyrene as a macromolecular crowding agent, an imprinted polymer recognizable to punicalagin had been successfully synthesized with punicalin as the dummy template. The resulting punicalin-imprinted polymer presented a remarkable selectivity to punicalagin with an imprinting factor of 3.17 even at extremely low consumption of the template (template/monomer ratio of 1:782). In contrast, the imprinted polymer synthesized without crowding agent, did not show any imprinting effect at so low template amount. The imprinted polymers made by combination of molecular crowding and virtual imprinting can be utilized for the fast separation of punicalagin from pomegranate husk extract after optimizing the protocol of solid-phase extraction with the recovery of 85.3 ± 1.2%.
authors.affiliation: Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Xinjiang, China.|State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Xinjiang, China.
authors.forename: Guang-Ying
authors.initials: GY
authors.lastname: Sun
authors.affiliation: Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Xinjiang, China.|State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Xinjiang, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Xinjiang, China.|State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Xinjiang, China.
authors.forename: Yu-Qin
authors.initials: YQ
authors.lastname: Luo
authors.affiliation: Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Xinjiang, China.|State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Xinjiang, China.
authors.forename: Yong-Xin
authors.initials: YX
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Xinjiang, China.|State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Xinjiang, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.affiliation: Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Xinjiang, China.|State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Xinjiang, China.
authors.forename: Zhao-Sheng
authors.initials: ZS
authors.lastname: Liu
authors.affiliation: Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Xinjiang, China.|State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Xinjiang, China.
authors.forename: Haji Akber
authors.initials: HA
authors.lastname: Aisa
mesh_terms: D047348:Hydrolyzable Tannins / Q000737:chemistry / Q000191:economics / Q000302:isolation & purification*; D029561:Lythraceae / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry / Q000191:economics; D054802:Molecular Imprinting / Q000191:economics*; D010936:Plant Extracts / Q000737:chemistry / Q000191:economics / Q000302:isolation & purification*; D011108:Polymers / Q000737:chemistry / Q000191:economics; D052616:Solid Phase Extraction / Q000191:economics
chemical_list: D047348:Hydrolyzable Tannins; D046911:Macromolecular Substances; D010936:Plant Extracts; D011108:Polymers; C115642:punicalagin
keywords: Dummy template; Macromolecular crowding; Molecular imprinting; Punicalagin; Punicalin