title: translin Is Required for Metabolic Regulation of Sleep.
abstract: Dysregulation of sleep or feeding has enormous health consequences. In humans, acute sleep loss is associated with increased appetite and insulin insensitivity, while chronically sleep-deprived individuals are more likely to develop obesity, metabolic syndrome, type II diabetes, and cardiovascular disease. Conversely, metabolic state potently modulates sleep and circadian behavior; yet, the molecular basis for sleep-metabolism interactions remains poorly understood. Here, we describe the identification of translin (trsn), a highly conserved RNA/DNA binding protein, as essential for starvation-induced sleep suppression. Strikingly, trsn does not appear to regulate energy stores, free glucose levels, or feeding behavior suggesting the sleep phenotype of trsn mutant flies is not a consequence of general metabolic dysfunction or blunted response to starvation. While broadly expressed in all neurons, trsn is transcriptionally upregulated in the heads of flies in response to starvation. Spatially restricted rescue or targeted knockdown localizes trsn function to neurons that produce the tachykinin family neuropeptide Leucokinin. Manipulation of neural activity in Leucokinin neurons revealed these neurons to be required for starvation-induced sleep suppression. Taken together, these findings establish trsn as an essential integrator of sleep and metabolic state, with implications for understanding the neural mechanism underlying sleep disruption in response to environmental perturbation.
authors.affiliation: Department of Biology, University of Nevada, Reno, NV 89557.|Department of Biological Sciences, Florida Atlantic University, John D MacArthur Campus, Jupiter, FL, USA.
authors.forename: Kazuma
authors.initials: K
authors.lastname: Murakami
authors.affiliation: Department of Biology, University of Nevada, Reno, NV 89557.|Department of Biological Sciences, Florida Atlantic University, John D MacArthur Campus, Jupiter, FL, USA.
authors.forename: Maria E
authors.initials: ME
authors.lastname: Yurgel
authors.affiliation: Department of Biological Sciences, Florida Atlantic University, John D MacArthur Campus, Jupiter, FL, USA.
authors.forename: Bethany A
authors.initials: BA
authors.lastname: Stahl
authors.affiliation: Department of Biology, SUNY Binghamton, Binghamton, NY 13902.
authors.forename: Pavel
authors.initials: P
authors.lastname: Masek
authors.affiliation: Department of Biology, University of Nevada, Reno, NV 89557.
authors.forename: Aradhana
authors.initials: A
authors.lastname: Mehta
authors.affiliation: Department of Biology, University of Nevada, Reno, NV 89557.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Heidker
authors.affiliation: Department of Biology, University of Nevada, Reno, NV 89557.|Department of Biological Sciences, Florida Atlantic University, John D MacArthur Campus, Jupiter, FL, USA.
authors.forename: Wesley
authors.initials: W
authors.lastname: Bollinger
authors.affiliation: Department of Biology, Hofstra University, Hempstead, NY, 11549.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Gingras
authors.affiliation: School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, South Korea.
authors.forename: Young-Joon
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Department of Metabolism and Aging, The Scripps Research Institute, Jupiter, FL 33458.
authors.forename: William W
authors.initials: WW
authors.lastname: Ja
authors.affiliation: University of Bern, Institute of Cell Biology, Bern, Switzerland, CH-3012.
authors.forename: Beat
authors.initials: B
authors.lastname: Suter
authors.affiliation: Department of Biology, Hofstra University, Hempstead, NY, 11549.|Division of Science, Penn State Berks, Reading, PA 19610.
authors.forename: Justin R
authors.initials: JR
authors.lastname: DiAngelo
authors.affiliation: Department of Biology, University of Nevada, Reno, NV 89557.|Department of Biological Sciences, Florida Atlantic University, John D MacArthur Campus, Jupiter, FL, USA.
authors.forename: Alex C
authors.initials: AC
authors.lastname: Keene
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000502:physiology*; D005247:Feeding Behavior; D006801:Humans; D023421:Models, Animal; D012890:Sleep; D013217:Starvation
chemical_list: D029721:Drosophila Proteins; C509504:trsn protein, Drosophila