title: SIGNAL MEDIATORS AT INDUCTION OF HEAT RESISTANCE OF WHEAT PLANTLETS BY SHORT-TERM HEATING.
abstract: The effects of functional interplay of calcium ions, reactive oxygen species (ROS) and nitric oxide (NO) in the cells of wheat plantlets roots (Triticum aestivum L.) at the induction of their heat resistance by a short-term influence of hyperthermia (heating at the temperature of 42 degrees C during 1 minute) have been investigated. The transitional increase of NO and H2O2 content, invoked by heating, was suppressed by the treatment of plantlets with the antagonists of calcium EGTA (chelator of exocellular calcium), lanthanum chloride (blocker of calcium channels of various types) and neomycin (inhibitor of phosphatidylinositol-dependent phospholipase C). The rise of hydrogen peroxide content, caused by hardening, was partially suppressed by the action of inhibitors of nitrate reductase (sodium wolframate) and NO-synthase (N(G)-nitro-L-arginine methyl ester--L-NAME), and the increasing of nitric oxide content was suppressed by the treatment of plants with the antioxidant ionol and with the scavenger of hydrogen peroxide (dimethylthiourea). These compounds and antagonists of calcium also partially removed the effect of the rise of plantlets' heat resistance, invoked by hardening heating. The conclusion on calcium's role in the activation of enzymatic systems, generating reactive oxygen species and nitric oxide, and on the functional interplay of these signal mediators at the induction of heat resistance of plantlets by hardening heating is made.
authors.forename: Yu V
authors.initials: YV
authors.lastname: Karpets
authors.forename: Yu E
authors.initials: YE
authors.lastname: Kolupaev
authors.forename: T O
authors.initials: TO
authors.lastname: Yastreb
mesh_terms: D000222:Adaptation, Physiological; D000975:Antioxidants / Q000494:pharmacology; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D065096:Calcium Chelating Agents / Q000494:pharmacology; D020013:Calcium Signaling; D002413:Cations, Divalent; D004533:Egtazic Acid / Q000494:pharmacology; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000378:metabolism; D007811:Lanthanum / Q000494:pharmacology; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009355:Neomycin / Q000494:pharmacology; D050901:Nitrate Reductase / Q000037:antagonists & inhibitors / Q000378:metabolism; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism; D054801:Phosphoinositide Phospholipase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism; D013890:Thiourea / Q000031:analogs & derivatives / Q000494:pharmacology; D014908:Triticum / Q000187:drug effects* / Q000378:metabolism; D017973:Tungsten Compounds / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D002121:Calcium Channel Blockers; D065096:Calcium Chelating Agents; D002413:Cations, Divalent; D017382:Reactive Oxygen Species; D017973:Tungsten Compounds; C028521:lanthanum chloride; D002084:Butylated Hydroxytoluene; D009569:Nitric Oxide; D004533:Egtazic Acid; C025399:sodium tungstate(VI); D007811:Lanthanum; C038983:1,3-dimethylthiourea; D006861:Hydrogen Peroxide; D019001:Nitric Oxide Synthase; D050901:Nitrate Reductase; D054801:Phosphoinositide Phospholipase C; D013890:Thiourea; D009355:Neomycin; D002118:Calcium; D019331:NG-Nitroarginine Methyl Ester