title: ORC interacts with THSC/TREX-2 and its subunits promote Nxf1 association with mRNP and mRNA export in Drosophila.
abstract: The origin recognition complex (ORC) of eukaryotes associates with the replication origins and initiates the pre-replication complex assembly. In the literature, there are several reports of interaction of ORC with different RNAs. Here, we demonstrate for the first time a direct interaction of ORC with the THSC/TREX-2 mRNA nuclear export complex. The THSC/TREX-2 was purified from the Drosophila embryonic extract and found to bind with a fraction of the ORC. This interaction occurred via several subunits and was essential for Drosophila viability. Also, ORC was associated with mRNP, which was facilitated by TREX-2. ORC subunits interacted with the Nxf1 receptor mediating the bulk mRNA export. The knockdown of Orc5 led to a drop in the Nxf1 association with mRNP, while Orc3 knockdown increased the level of mRNP-bound Nxf1. The knockdown of Orc5, Orc3 and several other ORC subunits led to an accumulation of mRNA in the nucleus, suggesting that ORC participates in the regulation of the mRNP export.
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia dvkopytova@gmail.com.
authors.forename: Daria
authors.initials: D
authors.lastname: Kopytova
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia.
authors.forename: Varvara
authors.initials: V
authors.lastname: Popova
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia.
authors.forename: Maria
authors.initials: M
authors.lastname: Kurshakova
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia.
authors.forename: Yulii
authors.initials: Y
authors.lastname: Shidlovskii
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia.
authors.forename: Elena
authors.initials: E
authors.lastname: Nabirochkina
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia.
authors.forename: Alexander
authors.initials: A
authors.lastname: Brechalov
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia.
authors.forename: Georgii
authors.initials: G
authors.lastname: Georgiev
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia sonjag@molbiol.edu.ru.
authors.forename: Sofia
authors.initials: S
authors.lastname: Georgieva
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D004330:Drosophila / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D029543:Nucleocytoplasmic Transport Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D051738:Origin Recognition Complex / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D021122:Protein Subunits / Q000037:antagonists & inhibitors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D034622:RNA Interference; D034443:RNA Transport; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D009687:Nuclear Proteins; D029543:Nucleocytoplasmic Transport Proteins; D051738:Origin Recognition Complex; D021122:Protein Subunits; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; C445086:SBR protein, Drosophila; C026452:messenger ribonucleoprotein; C527103:xmas protein, Drosophila