title: Lipoteichoic Acid Isolated from Weissella cibaria Increases Cytokine Production in Human Monocyte-Like THP-1 Cells and Mouse Splenocytes.
abstract: Lactic acid bacteria (LAB) have beneficial effects on intestinal health and skin diseases. Lipoteichoic acid (LTA), a cell wall component of gram-positive bacteria, is known to induce the production of several cytokines such as TNF-α, IL-1β, and IL-8 and affect the intestinal microflora, anti-aging, sepsis, and cholesterol level. In this study, Weissella cibaria was isolated from Indian dairy products, and we examined its immune-enhancing effects. Live and heatkilled W. cibaria did not induce the secretion of immune-related cytokines, whereas LTA isolated from W. cibaria (cLTA) significantly increased the secretion of TNF-α, IL-1β, and IL-6 in a dose-dependent manner. cLTA increased the phosphorylation of nuclear factor kappalight-chain-enhancer of activated B cells, p38 mitogen-activated protein kinases, and c-Jun N-terminal kinases in THP-1 cells. The secretion of TNF-α and IL-6 was also increased in the cLTA-treated mouse splenocytes. These results suggest that cLTA, but not W. cibaria whole cells, has immune-boosting potential and can be used to treat immunosuppression diseases.
authors.affiliation: Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Yi-Fan
authors.initials: YF
authors.lastname: Hong
authors.affiliation: Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Yoon-Doo
authors.initials: YD
authors.lastname: Lee
authors.affiliation: Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Jae-Yeon
authors.initials: JY
authors.lastname: Park
authors.affiliation: Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Seongjae
authors.initials: S
authors.lastname: Kim
authors.affiliation: Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Youn-Woo
authors.initials: YW
authors.lastname: Lee
authors.affiliation: Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Boram
authors.initials: B
authors.lastname: Jeon
authors.affiliation: Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Deepa
authors.initials: D
authors.lastname: Jagdish
authors.affiliation: Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 17104, Republic of Korea.|Skin Biotechnology Center, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Hangeun
authors.initials: H
authors.lastname: Kim
authors.affiliation: Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 17104, Republic of Korea.|Skin Biotechnology Center, Kyung Hee University, Yongin 17104, Republic of Korea.|RNA Inc., #308 College of Life Science, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Dae Kyun
authors.initials: DK
authors.lastname: Chung
mesh_terms: D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis*; D006801:Humans; D056747:Immunomodulation / Q000187:drug effects; D018836:Inflammation Mediators / Q000378:metabolism; D008070:Lipopolysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D010766:Phosphorylation; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013154:Spleen / Q000166:cytology*; D013682:Teichoic Acids / Q000302:isolation & purification / Q000494:pharmacology*; D058836:Weissella / Q000737:chemistry* / Q000145:classification / Q000235:genetics
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D016328:NF-kappa B; D012336:RNA, Ribosomal, 16S; D013682:Teichoic Acids; C009900:lipoteichoic acid; D020928:Mitogen-Activated Protein Kinases
keywords: Immune regulation; Weissella cibaria; cytokine; lipoteichoic acid