title: Jellyfish-associated bacterial communities and bacterioplankton in Indonesian Marine lakes.
abstract: In the present study, we compared communities of bacteria in two jellyfish species (the 'golden' jellyfish Mastigias cf.papua and the box jellyfish Tripedalia cf.cystophora) and water in three marine lakes located in the Berau region of northeastern Borneo, Indonesia. Jellyfish-associated bacterial communities were compositionally distinct and less diverse than bacterioplankton communities. Alphaproteobacteria, Gammaproteobacteria, Synechococcophycidae and Flavobacteriia were the most abundant classes in water. Jellyfish-associated bacterial communities were dominated by OTUs assigned to the Gammaproteobacteria (family Endozoicimonaceae), Mollicutes, Spirochaetes and Alphaproteobacteria (orders Kiloniellales and Rhodobacterales). Mollicutes were mainly restricted to Mastigias whereas Spirochaetes and the order Kiloniellales were most abundant in Tripedalia hosts. The most abundant OTU overall in jellyfish hosts was assigned to the family Endozoicimonaceae and was highly similar to organisms in Genbank obtained from various hosts including an octocoral, bivalve and fish species. Other abundant OTUs included an OTU assigned to the order Entomoplasmatales and mainly found in Mastigias hosts and OTUs assigned to the Spirochaetes and order Kiloniellales and mainly found in Tripedalia hosts. The low sequence similarity of the Entomoplasmatales OTU to sequences in Genbank suggests that it may be a novel lineage inhabiting Mastigias and possibly restricted to marine lakes.
authors.affiliation: Department of Biology, CESAM, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal cleary@ua.pt.
authors.forename: Daniel F R
authors.initials: DF
authors.lastname: Cleary
authors.affiliation: Department of Marine Zoology, Naturalis Biodiversity Center, Darwinweg 2, 2333 CR Leiden, the Netherlands Wageningen University and Research Centre, Marine Animal Ecology Group, PO Box 338, 6700 AH Wageningen, the Netherlands Department of Environmental Science, Policy, and Management, University of California Berkeley, 130 Mulford Hall, Berkeley CA 94720-3114, USA.
authors.forename: Leontine E
authors.initials: LE
authors.lastname: Becking
authors.affiliation: Department of Biology, CESAM, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
authors.forename: Ana R M
authors.initials: AR
authors.lastname: Polónia
authors.affiliation: Department of Biology, CESAM, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
authors.forename: Rossana M
authors.initials: RM
authors.lastname: Freitas
authors.affiliation: Department of Biology, CESAM, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
authors.forename: Newton C M
authors.initials: NC
authors.lastname: Gomes
mesh_terms: D000818:Animals; D059001:Aquatic Organisms / Q000382:microbiology; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D003063:Cnidaria / Q000382:microbiology*; D007214:Indonesia; D060106:Lakes / Q000382:microbiology*; D010933:Plankton / Q000145:classification*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Mastigias; Tripedalia; anchialine; bacterioplankton; pyrosequencing