title: Mn(2+)-doped NaYF4:Yb/Er upconversion nanoparticle-based electrochemiluminescent aptasensor for bisphenol A.
abstract: A novel aptasensor labeled with Mn(2+)-doped NaYF4:Yb/Er upconversion nanoparticles (NaYF4:Yb,Er/Mn UCNPs) was employed in electrogenerated chemiluminescence (ECL) for the sensitive detection of bisphenol A (BPA). The ECL aptasensor was assembled by immobilizing the thiolated aptamers of BPA covalently on a gold nanoparticle (AuNPs)-modified electrode and pairing with complementary DNA labeled with NaYF4:Yb,Er/Mn UCNPs. The ECL aptasensor can not only rapidly and accurately detect BPA concentrations from 0.05 to 100 ng/mL with a detection limit of 0.037 ng/mL but also provides a new platform for ECL applications based on the use of upconversion nanoparticles as a promising alternative material. Graphical Abstract The NaYF4:Yb,Er/Mn UCNPs combining with the BPA aptamer serving as recognition elements create a ECL platform for the sensitive detection of bisphenol A. The change in ECL signals induced by aptamer-target interactions was measured and a significant decrease in intensity was found on interaction with BPA in the concentration range of 0.05 to 100 ng/mL.
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China.
authors.forename: Shijia
authors.initials: S
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China.
authors.forename: Nuo
authors.initials: N
authors.lastname: Duan
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China. wangzp1974@hotmail.com.
authors.forename: Zhouping
authors.initials: Z
authors.lastname: Wang
mesh_terms: D001559:Benzhydryl Compounds / Q000032:analysis*; D004563:Electrochemistry; D004871:Erbium / Q000737:chemistry*; D005459:Fluorides / Q000737:chemistry*; D057230:Limit of Detection; D049449:Luminescence; D008345:Manganese / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D015018:Ytterbium / Q000737:chemistry*; D015019:Yttrium / Q000737:chemistry*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004871:Erbium; D005459:Fluorides; D008345:Manganese; D010636:Phenols; D015018:Ytterbium; D015019:Yttrium; C525362:sodium yttriumtetrafluoride; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Aptamer; Bisphenol A; ECL; NaYF4:Yb,Er/Mn UCNPs