title: Further increased production of free fatty acids by overexpressing a predicted transketolase gene of the pentose phosphate pathway in Aspergillus oryzae faaA disruptant.
abstract: Free fatty acids are useful as source materials for the production of biodiesel fuel and various chemicals such as pharmaceuticals and dietary supplements. Previously, we attained a 9.2-fold increase in free fatty acid productivity by disrupting a predicted acyl-CoA synthetase gene (faaA, AO090011000642) in Aspergillus oryzae. In this study, we achieved further increase in the productivity by overexpressing a predicted transketolase gene of the pentose phosphate pathway in the faaA disruptant. The A. oryzae genome is predicted to have three transketolase genes and overexpression of AO090023000345, one of the three genes, resulted in phenotypic change and further increase (corresponding to an increased production of 0.38 mmol/g dry cell weight) in free fatty acids at 1.4-fold compared to the faaA disruptant. Additionally, the biomass of hyphae increased at 1.2-fold by the overexpression. As a result, free fatty acid production yield per liter of liquid culture increased at 1.7-fold by the overexpression.
authors.affiliation: a Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) , Sapporo , Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Tamano
authors.affiliation: a Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) , Sapporo , Japan.
authors.forename: Ai
authors.initials: A
authors.lastname: Miura
mesh_terms: D001236:Aspergillus oryzae / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D003066:Coenzyme A Ligases / Q000096:biosynthesis / Q000235:genetics*; D005230:Fatty Acids, Nonesterified / Q000096:biosynthesis* / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D025301:Hyphae / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D010427:Pentose Phosphate Pathway / Q000235:genetics; D014174:Transketolase / Q000235:genetics
chemical_list: D005230:Fatty Acids, Nonesterified; D005656:Fungal Proteins; D014174:Transketolase; D003066:Coenzyme A Ligases
keywords: Aspergillus oryzae faaA disruptant; free fatty acid; pentose phosphate pathway; productivity; transketolase gene overexpression