title: Synthesis of adsorbents with dendronic structures for protein hydrophobic interaction chromatography.
abstract: Here, we introduced a new technology based on the incorporation of dendrons-branched chemical structures-onto supports for synthesis of HIC adsorbents. In doing so we studied the synthesis and performance of these novel HIC dendron-based adsorbents. The adsorbents were synthesized in a facile two-step reaction. First, Sepharose 4FF (R) was chemically modified with polyester dendrons of different branching degrees i.e. third (G3) or fifth (G5) generations. Then, butyl-end valeric acid ligands were coupled to dendrons via ester bond formation. UV-vis spectrophotometry and FTIR analyses of the modified resins confirmed the presence of the dendrons and their ligands on them. Inclusion of dendrons allowed the increment of ligand density, 82.5 ± 11 and 175.6 ± 5.7 μmol ligand/mL resin for RG3 and RG5, respectively. Static adsorption capacity of modified resins was found to be ∼ 60 mg BSA/mL resin. Interestingly, dynamic binding capacity was higher at high flow rates, 62.5 ± 0.8 and 58.0 ± 0.5mg/mL for RG3 and RG5, respectively. RG3 was able to separate lipase, β-lactoglobulin and α-chymotrypsin selectively as well as fractionating of a whole proteome from yeast. This innovative technology will improve the existing HIC resin synthesis methods. It will also allow the reduction of the amount of adsorbent used in a chromatographic procedure and thus permit the use of smaller columns resulting in faster processes. Furthermore, this method could potentially be considered as a green technology since both, dendrons and ligands, are formed by ester bonds that are more biodegradable allowing the disposal of used resin waste in a more ecofriendly manner when compared to other exiting resins.
authors.affiliation: School of Engineering and Sciences, Tecnologico de Monterrey, Ave. Eugenio Garza Sada 2501 Sur, Monterrey, NL 64849, Mexico. Electronic address: matago24@gmail.com.
authors.forename: Marco A
authors.initials: MA
authors.lastname: Mata-Gómez
authors.affiliation: Izmir Institute of Technology, Department of Bioengineering, Urla, 35430 İzmir, Turkey. Electronic address: senaymn@gmail.com.
authors.forename: Sena
authors.initials: S
authors.lastname: Yaman
authors.affiliation: School of Engineering and Sciences, Tecnologico de Monterrey, Ave. Eugenio Garza Sada 2501 Sur, Monterrey, NL 64849, Mexico. Electronic address: valencia@itesm.mx.
authors.forename: Jesus A
authors.initials: JA
authors.lastname: Valencia-Gallegos
authors.affiliation: Izmir Institute of Technology, Department of Food Engineering, Urla, 35430 İzmir, Turkey. Electronic address: canantari@iyte.edu.tr.
authors.forename: Canan
authors.initials: C
authors.lastname: Tari
authors.affiliation: School of Engineering and Sciences, Tecnologico de Monterrey, Ave. Eugenio Garza Sada 2501 Sur, Monterrey, NL 64849, Mexico. Electronic address: mrito@itesm.mx.
authors.forename: Marco
authors.initials: M
authors.lastname: Rito-Palomares
authors.affiliation: School of Engineering and Sciences, Tecnologico de Monterrey, Ave. Eugenio Garza Sada 2501 Sur, Monterrey, NL 64849, Mexico. Electronic address: jose_gonzalez@itesm.mx.
authors.forename: José
authors.initials: J
authors.lastname: González-Valdez
mesh_terms: D000327:Adsorption; D002845:Chromatography / Q000379:methods*; D002918:Chymotrypsin / Q000737:chemistry; D050091:Dendrimers / Q000737:chemistry* / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D007782:Lactoglobulins / Q000737:chemistry; D008024:Ligands; D020543:Proteome / Q000737:chemistry; D012685:Sepharose / Q000737:chemistry
chemical_list: D050091:Dendrimers; D007782:Lactoglobulins; D008024:Ligands; D020543:Proteome; D012685:Sepharose; D002918:Chymotrypsin; C117608:alpha-chymotrypsin
keywords: Adsorbent; Dendrons; Hydrophobic interaction chromatography; Resin