title: Functional characterization of CYP52G3 from Aspergillus oryzae and its application for bioconversion and synthesis of hydroxyl flavanone and steroids.
abstract: Aspergillus oryzae is a fungus widely used in traditional Japanese fermentation industries. Cytochrome P450 (CYP) proteins are ubiquitously distributed in nature and display a broad range of enzymatic activities. A novel CYP52 (CYP52G3) gene was found in A. oryzae. In this study, we report the functional characterization of CYP52G3. The recombinant protein was expressed heterologously in Escherichia coli, and its membrane fraction isolated. CYP52G3 showed activities for 7-ethoxycoumarin and α-naphtoflavone. Furthermore, CYP52G3 hydroxylated flavanone at the 4' and 6 position and metabolized some hydroxyl-flavanones and steroids. Bioconversion experiments indicated that CYP52G3 could convert flavanone and testosterone in a synthetic medium. The conversion rates of flavanone and testosterone at 24 H were 50% and 70%, respectively. These results support that CYP52G3 could prove a useful enzyme for the efficient production of new compounds from flavonoids and steroids.
authors.affiliation: Laboratory of Biochemistry, Graduate School of Agricultural Science, Kobe University, Kobe, Japan.
authors.forename: Tomohide
authors.initials: T
authors.lastname: Uno
authors.affiliation: Laboratory of Biochemistry, Graduate School of Agricultural Science, Kobe University, Kobe, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yanase
authors.affiliation: Functional Analysis of Environmental Genes, Research Center for Environmental Genomics, Kobe University, Kobe, Japan.
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Imaishi
mesh_terms: D001236:Aspergillus oryzae / Q000201:enzymology*; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry*; D044950:Flavanones / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D013739:Testosterone / Q000737:chemistry*
chemical_list: D044950:Flavanones; D005656:Fungal Proteins; D013739:Testosterone; D003577:Cytochrome P-450 Enzyme System
keywords: Aspergillus oryzae; CYP52; bioconversion; flavanone; steroid