title: Evaluation of Protective Effects of Bioactive Phytochemicals Against Methotrexate in Salmonella typhimurium TA1535/pSK1002 Coupled with Micronucleus Assay.
abstract: We evaluated the antimutagenic effects of 10 kinds of bioactive phytochemicals and some phytochemical combinations against methotrexate (MTX)-induced genotoxicity by the umu test in Salmonella typhimurium TA1535/pSK1002 combined with a micronucleus assay. We observed that allicin, proanthocyanidins, polyphenols, eleutherosides, and isoflavones had higher antimutagenic activities than the other five types of bioactive phytochemicals. At the highest dose tested, MTX-induced genotoxicity was inhibited by 25%-75%. Kunming mice treated by MTX along with bioactive phytochemical combinations showed significant reduction in micronucleus induction and sperm abnormality rate (P<0.01). These results indicate that bioactive phytochemical combinations can be potentially used as new cytoprotectors.
authors.affiliation: College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, Henan, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wu
authors.affiliation: College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, Henan, China.
authors.forename: Shao Bin
authors.initials: SB
authors.lastname: Gu
authors.affiliation: Rice Research Institute, Anhui Academy of Agricultural Science, Hefei 230031, Anhui, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.affiliation: China University of Petroleum, Qingdao 257061, Shandong, China.
authors.forename: Jia Yi
authors.initials: JY
authors.lastname: He
authors.affiliation: Rice Research Institute, Anhui Academy of Agricultural Science, Hefei 230031, Anhui, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Rice Research Institute, Anhui Academy of Agricultural Science, Hefei 230031, Anhui, China.
authors.forename: Jian Bo
authors.initials: JB
authors.lastname: Yang
mesh_terms: D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000009:adverse effects*; D019610:Cytoprotection; D004353:Drug Evaluation, Preclinical; D005260:Female; D008297:Male; D008727:Methotrexate / Q000009:adverse effects*; D051379:Mice; D015162:Micronucleus Tests; D008517:Phytotherapy; D010936:Plant Extracts; D011897:Random Allocation; D012486:Salmonella typhimurium
chemical_list: D000964:Antimetabolites, Antineoplastic; D010936:Plant Extracts; D008727:Methotrexate