title: Enhanced oxidative stress in adipose tissue from diabetic mice, possible contribution of glycated albumin.
abstract: Although enhanced oxidative stress and proteotoxicity constitute major contributors to the pathogenesis of multiple diseases, there is limited understanding of its role in adipose tissue. Here, we aimed at evaluating oxidative stress biomarkers in adipocytes from diabetic/obese db/db mice. The current study revealed that reactive oxygen species production was upregulated in adipocytes, together with lipid peroxidation 4-hydroxynonenal accumulation, and altered proteolytic and antioxidant activities. In parallel, acute exposure of 3T3L1 adipocyte cell lines to glycated albumin (known to be enhanced with diabetes) also elicited intracellular free radical formation. Our data provide novel insights into redox and proteolytic homeostasis in adipocytes.
authors.affiliation: Inserm, UMR 1188 Diabète athérothrombose Thérapies Réunion Océan Indien (DéTROI), plateforme CYROI, Sainte-Clotilde F-97490, France; Université de La Réunion, UMR 1188, Sainte-Clotilde F-97490, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Boyer
authors.affiliation: Inserm, UMR 1188 Diabète athérothrombose Thérapies Réunion Océan Indien (DéTROI), plateforme CYROI, Sainte-Clotilde F-97490, France; Université de La Réunion, UMR 1188, Sainte-Clotilde F-97490, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Diotel
authors.affiliation: Inserm, UMR 1188 Diabète athérothrombose Thérapies Réunion Océan Indien (DéTROI), plateforme CYROI, Sainte-Clotilde F-97490, France; Université de La Réunion, UMR 1188, Sainte-Clotilde F-97490, France.
authors.forename: Dorothée
authors.initials: D
authors.lastname: Girard
authors.affiliation: Inserm, UMR 1188 Diabète athérothrombose Thérapies Réunion Océan Indien (DéTROI), plateforme CYROI, Sainte-Clotilde F-97490, France; Université de La Réunion, UMR 1188, Sainte-Clotilde F-97490, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Rondeau
authors.affiliation: Cardio-Metabolic Research Group (CMRG), Department of Physiological Sciences, Stellenbosch University, Merriman Avenue, Stellenbosch 7600, South Africa.
authors.forename: M Faadiel
authors.initials: MF
authors.lastname: Essop
authors.affiliation: Inserm, UMR 1188 Diabète athérothrombose Thérapies Réunion Océan Indien (DéTROI), plateforme CYROI, Sainte-Clotilde F-97490, France; Université de La Réunion, UMR 1188, Sainte-Clotilde F-97490, France. Electronic address: Emmanuel.bourdon@univ-reunion.fr.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Bourdon
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism; D000273:Adipose Tissue / Q000503:physiopathology*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D005609:Free Radicals / Q000378:metabolism; D017127:Glycation End Products, Advanced; D006720:Homozygote; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D012709:Serum Albumin / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D000093423:Glycated Serum Albumin
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D005609:Free Radicals; D017127:Glycation End Products, Advanced; D017382:Reactive Oxygen Species; D012709:Serum Albumin; D013438:Sulfhydryl Compounds; D000093423:Glycated Serum Albumin
keywords: Diabetes; Glycated albumin; Hyperglycemia; Oxidative stress; Proteasome