title: Sulforaphane Attenuates Contrast-Induced Nephropathy in Rats via Nrf2/HO-1 Pathway.
abstract: Background. Oxidative stress plays an important role in the pathogenesis of contrast-induced nephropathy (CIN). The aim of this study was to investigate the antioxidant effects of sulforaphane (SFN) in a rat model of CIN and a cell model of oxidative stress in HK2 cells. Methods. Rats were randomized into four groups (n = 6 per group): control group, Ioversol group (Ioversol-induced CIN), Ioversol + SFN group (CIN rats pretreated with SFN), and SFN group (rats treated with SFN). Renal function tests, malondialdehyde (MDA), and reactive oxygen species (ROS) were measured. Western blot, real-time polymerase chain reaction analysis, and immunohistochemical analysis were performed for nuclear factor erythroid-derived 2-like 2 (Nrf2) and heme oxygenase-1 (HO-1) detection. Results. Serum blood urea nitrogen (BUN), creatinine, and renal tissue MDA were increased after contrast exposure. Serum BUN, creatinine, and renal tissue MDA were decreased in the Ioversol + SFN group as compared with those in the Ioversol group. SFN increased the expression of Nrf2 and HO-1 in CIN rats and in Ioversol-induced injury HK2 cells. SFN increased cell viability and attenuated ROS level in vitro. Conclusions. SFN attenuates experimental CIN in vitro and in vivo. This effect is suggested to activate the Nrf2 antioxidant defenses pathway.
authors.affiliation: Department of Nephrology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong 510630, China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Radiation Oncology, Shenzhen People's Hospital, Second Clinical Medicine College of Jinan University, Shenzhen, Guangdong 518020, China.
authors.forename: Guixiang
authors.initials: G
authors.lastname: Liao
authors.affiliation: Department of Nephrology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong 510630, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Department of Nephrology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong 510630, China.
authors.forename: Daoyuan
authors.initials: D
authors.lastname: Lv
authors.affiliation: National Centre for Sensor Research/BioAnalytical Sciences, Dublin City University, Dublin 9, Ireland.
authors.forename: Harry
authors.initials: H
authors.lastname: Holthfer
authors.affiliation: Department of Nephrology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong 510630, China.
authors.forename: Hequn
authors.initials: H
authors.lastname: Zou
mesh_terms: D000818:Animals; D003287:Contrast Media / Q000009:adverse effects* / Q000494:pharmacology; D005260:Female; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism*; D017879:Isothiocyanates / Q000494:pharmacology*; D007674:Kidney Diseases / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D051267:NF-E2-Related Factor 2 / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects*; D013454:Sulfoxides; D014283:Triiodobenzoic Acids / Q000009:adverse effects* / Q000494:pharmacology
chemical_list: D003287:Contrast Media; D017879:Isothiocyanates; D051267:NF-E2-Related Factor 2; C495637:Nfe2l2 protein, rat; D013454:Sulfoxides; D014283:Triiodobenzoic Acids; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat; C016766:sulforaphane; C054871:ioversol