title: Solid-phase extraction based on ground methacrylate monolith modified with gold nanoparticles for isolation of proteins.
abstract: In this study, a novel polymeric material functionalized with gold nanoparticles (AuNPs) was prepared as solid-phase extraction (SPE) sorbent for isolation of proteins. The sorbent was synthesized from a powdered poly(glycidyl-co-ethylene dimethacrylate) monolith, and modified with ammonia, followed by immobilization of AuNPs on the pore surface of the material. To evaluate the performance of this SPE support, proteins were selected as test solutes, being the extraction conditions and other parameters (loading capacity and regenerative ability of sorbent) established. The results indicated that this sorbent could be employed to selectively capture proteins according to their pI, on the basis of the strong affinity of these biomacromolecules towards to AuNPs surface. The applicability of this sorbent was demonstrated by isolating protein species of interest (bovine serum albumin, cytochrome c and lectins in European mistletoe leaves), followed by SDS-PAGE analysis.
authors.affiliation: Department of Analytical Chemistry, University of Valencia, C. Doctor Moliner 50, E-46100 Burjassot, Valencia, Spain. Electronic address: maria.vergara@uv.es.
authors.forename: María
authors.initials: M
authors.lastname: Vergara-Barberán
authors.affiliation: Department of Analytical Chemistry, University of Valencia, C. Doctor Moliner 50, E-46100 Burjassot, Valencia, Spain.
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Lerma-García
authors.affiliation: Department of Analytical Chemistry, University of Valencia, C. Doctor Moliner 50, E-46100 Burjassot, Valencia, Spain.
authors.forename: Ernesto Francisco
authors.initials: EF
authors.lastname: Simó-Alfonso
authors.affiliation: Department of Analytical Chemistry, University of Valencia, C. Doctor Moliner 50, E-46100 Burjassot, Valencia, Spain. Electronic address: jmherrer@uv.es.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Herrero-Martínez
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D011506:Proteins / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D008689:Methacrylates; D011506:Proteins; D006046:Gold
keywords: Glycidyl methacrylate monolith; Gold nanoparticles; Proteins; Solid-phase extraction