title: Optimization by response surface methodology of lutein recovery from paprika leaves using accelerated solvent extraction.
abstract: In this study, we used response surface methodology (RSM) to optimize the extraction conditions for recovering lutein from paprika leaves using accelerated solvent extraction (ASE). The lutein content was quantitatively analyzed using a UPLC equipped with a BEH C18 column. A central composite design (CCD) was employed for experimental design to obtain the optimized combination of extraction temperature (°C), static time (min), and solvent (EtOH, %). The experimental data obtained from a twenty sample set were fitted to a second-order polynomial equation using multiple regression analysis. The adjusted coefficient of determination (R(2)) for the lutein extraction model was 0.9518, and the probability value (p=0.0000) demonstrated a high significance for the regression model. The optimum extraction conditions for lutein were temperature: 93.26°C, static time: 5 min, and solvent: 79.63% EtOH. Under these conditions, the predicted extraction yield of lutein was 232.60 μg/g.
authors.affiliation: Department of Food and Nutrition, Chung-Ang University, Anseoung-si, Gyeonggi-do 456-756, Republic of Korea.
authors.forename: Jae-Hyun
authors.initials: JH
authors.lastname: Kang
authors.affiliation: Food and Nutrition in Home Economics, Korea National Open University, 169 Dongsung-Dong, Jongno-Gu, Seoul 110-791, Republic of Korea.
authors.forename: Suna
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Food and Nutrition, Chung-Ang University, Anseoung-si, Gyeonggi-do 456-756, Republic of Korea. Electronic address: bkmoon@cau.ac.kr.
authors.forename: BoKyung
authors.initials: B
authors.lastname: Moon
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D052160:Benzothiazoles / Q000737:chemistry; D002212:Capsicum / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods; D014975:Lutein / Q000032:analysis* / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D012044:Regression Analysis; D012997:Solvents / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D010936:Plant Extracts; D012997:Solvents; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D014975:Lutein
keywords: Accelerated solvent extraction (ASE); Lutein; Paprika leaves; Response surface method (RSM); Ultra performance liquid chromatography (UPLC)