title: The unfolded protein response and programmed cell death are induced by expression of Garlic virus X p11 in Nicotiana benthamiana.
abstract: Garlic virus X (GarVX) ORF3 encodes a p11 protein, which contributes to virus cell-to-cell movement and forms granules on the endoplasmic reticulum (ER) in Nicotiana benthamiana. Expression of p11 either from a binary vector, PVX or TMV induced ER stress and the unfolded protein response (UPR), as demonstrated by an increase in transcription of the ER luminal binding protein (BiP) and bZIP60 genes. UPR-related programmed cell death (PCD) was elicited by PVX : p11 or TMV : p11 in systemic infected leaves. Examination of p11 mutants with deletions of two transmembrane domains (TM) revealed that both were required for generating granules and for inducing necrosis. TRV-based VIGS was used to investigate the correlation between bZIP60 expression and p11-induced UPR-related PCD. Less necrosis was observed on local and systemic leaves of bZIP60 knockdown plants when infected with PVXp11, suggesting that bZIP60 plays an important role in the UPR-related PCD response to p11 in N. benthamiana.
authors.affiliation: College of Plant Protection, Hunan Agricultural University, Changsha 410128, PR China.|State Key laboratory Breeding Base for Sustainable Control of Pest and Disease, Key Laboratory of Biotechnology in Plant Protection, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Tibet Agricultural and Animal Husbandry College, Linzhi 860000, PR China.
authors.forename: Mingyuan
authors.initials: M
authors.lastname: Yin
authors.affiliation: Virus-free Seedling Research Institute of Heilongjiang Academy of Agricultural Sciences, No. 368, Xuefu Road, Harbin 150086, PR China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering of Ministry of Education, Guizhou University, Guiyang 550025, PR China.
authors.forename: Binghua
authors.initials: B
authors.lastname: Chen
authors.affiliation: Department of Plant Protection, Shenyang Agriculture University, Shenyang 110161, PR China.
authors.forename: Xue
authors.initials: X
authors.lastname: Yang
authors.affiliation: State Key laboratory Breeding Base for Sustainable Control of Pest and Disease, Key Laboratory of Biotechnology in Plant Protection, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.
authors.forename: Jiejun
authors.initials: J
authors.lastname: Peng
authors.affiliation: State Key laboratory Breeding Base for Sustainable Control of Pest and Disease, Key Laboratory of Biotechnology in Plant Protection, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.
authors.forename: Hongying
authors.initials: H
authors.lastname: Zheng
authors.affiliation: State Key laboratory Breeding Base for Sustainable Control of Pest and Disease, Key Laboratory of Biotechnology in Plant Protection, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Zhao
authors.affiliation: State Key laboratory Breeding Base for Sustainable Control of Pest and Disease, Key Laboratory of Biotechnology in Plant Protection, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.affiliation: State Key laboratory Breeding Base for Sustainable Control of Pest and Disease, Key Laboratory of Biotechnology in Plant Protection, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.
authors.forename: Chulang
authors.initials: C
authors.lastname: Yu
authors.affiliation: The James Hutton Institute, Cell and Molecular Sciences Group, Invergowrie, Dundee DD2 5DA, UK.
authors.forename: Stuart
authors.initials: S
authors.lastname: MacFarlane
authors.affiliation: Tibet Agricultural and Animal Husbandry College, Linzhi 860000, PR China.
authors.forename: Jianqing
authors.initials: J
authors.lastname: He
authors.affiliation: Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key laboratory Breeding Base for Sustainable Control of Pest and Disease, Key Laboratory of Biotechnology in Plant Protection, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Plant Protection, Hunan Agricultural University, Changsha 410128, PR China.
authors.forename: Liangying
authors.initials: L
authors.lastname: Dai
authors.affiliation: State Key laboratory Breeding Base for Sustainable Control of Pest and Disease, Key Laboratory of Biotechnology in Plant Protection, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Yan
mesh_terms: D017209:Apoptosis; D059865:Endoplasmic Reticulum Stress; D052677:Flexiviridae / Q000235:genetics / Q000472:pathogenicity*; D010935:Plant Diseases / Q000821:virology*; D010940:Plant Proteins / Q000032:analysis; D014026:Nicotiana / Q000821:virology*; D056811:Unfolded Protein Response; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D010940:Plant Proteins; D014764:Viral Proteins