title: Detection of Myoglobin with an Open-Cavity-Based Label-Free Photonic Crystal Biosensor.
abstract: The label-free detection of one of the cardiac biomarkers, myoglobin, using a photonic-crystal-based biosensor in a total-internal-reflection configuration (PC-TIR) is presented in this paper. The PC-TIR sensor possesses a unique open optical microcavity that allows for several key advantages in biomolecular assays. In contrast to a conventional closed microcavity, the open configuration allows easy functionalization of the sensing surface for rapid biomolecular binding assays. Moreover, the properties of PC structures make it easy to be designed and engineered for operating at any optical wavelength. Through fine design of the photonic crystal structure, biochemical modification of the sensor surface, and integration with a microfluidic system, we have demonstrated that the detection sensitivity of the sensor for myoglobin has reached the clinically significant concentration range, enabling potential usage of this biosensor for diagnosis of acute myocardial infarction. The real-time response of the sensor to the myoglobin binding may potentially provide point-of-care monitoring of patients and treatment effects.
authors.affiliation: University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA.
authors.forename: Bailin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA.
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Tamez-Vela
authors.affiliation: University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA.
authors.forename: Steven
authors.initials: S
authors.lastname: Solis
authors.affiliation: University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA.
authors.forename: Gilbert
authors.initials: G
authors.lastname: Bustamante
authors.affiliation: University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA.
authors.forename: Ralph
authors.initials: R
authors.lastname: Peterson
authors.affiliation: University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA.
authors.forename: Shafiqur
authors.initials: S
authors.lastname: Rahman
authors.affiliation: University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA.
authors.forename: Andres
authors.initials: A
authors.lastname: Morales
authors.affiliation: University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA.
authors.forename: Liang
authors.initials: L
authors.lastname: Tang
authors.affiliation: University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA.
authors.forename: Jing Yong
authors.initials: JY
authors.lastname: Ye