title: Fish oil supplementation suppresses resistance exercise and feeding-induced increases in anabolic signaling without affecting myofibrillar protein synthesis in young men.
abstract: Fish oil (FO) supplementation potentiates muscle protein synthesis (MPS) in response to a hyperaminoacidemic-hyperinsulinemic infusion. Whether FO supplementation potentiates MPS in response to protein ingestion or when protein ingestion is combined with resistance exercise (RE) remains unknown. In a randomized, parallel group design, 20 healthy males were randomized to receive 5 g/day of either FO or coconut oil control (CO) for 8 weeks. After supplementation, participants performed a bout of unilateral RE followed by ingestion of 30 g of whey protein. Skeletal muscle biopsies were obtained before and after supplementation for assessment of muscle lipid composition and relevant protein kinase activities. Infusion of L-[ring-(13)C6] phenylalanine was used to measure basal myofibrillar MP Sat rest (REST), in a nonexercised leg following protein ingestion (FED) and following RE and protein ingestion (FEDEX).MPS was significantly elevated above REST during FEDEX in both the FO and CO groups, but there was no effect of supplementation. There was a significant increase in MPS in both groups above REST during FED but no effect of supplementation. Supplementation significantly decreased pan PKB activity at RESTin the FO group but not the CO group. There was a significant increase from REST at post-RE for PKB and AMPKα2 activity in the CO group but not in the FO group. In FEDEX, there was a significant increase in p70S6K1 activity from REST at 3 h in the CO group only. These data highlight that 8 weeks of FO supplementation alters kinase signaling activity in response to RE plus protein ingestion without influencing MPS.
authors.affiliation: Exercise and Metabolism Research Group, Department of Kinesiology, McMaster University, Hamilton, Ontario, Canada Health and Exercise Sciences Research Group, School of Sport University of Stirling, Stirling, UK mcglorc@mcmaster.ca.
authors.forename: Chris
authors.initials: C
authors.lastname: McGlory
authors.affiliation: Health and Exercise Sciences Research Group, School of Sport University of Stirling, Stirling, UK.
authors.forename: Sophie L
authors.initials: SL
authors.lastname: Wardle
authors.affiliation: Health and Exercise Sciences Research Group, School of Sport University of Stirling, Stirling, UK.
authors.forename: Lindsay S
authors.initials: LS
authors.lastname: Macnaughton
authors.affiliation: Health and Exercise Sciences Research Group, School of Sport University of Stirling, Stirling, UK.
authors.forename: Oliver C
authors.initials: OC
authors.lastname: Witard
authors.affiliation: Health and Exercise Sciences Research Group, School of Sport University of Stirling, Stirling, UK.
authors.forename: Fraser
authors.initials: F
authors.lastname: Scott
authors.affiliation: Nutrition Group, Institute of Aquaculture, School of Natural Sciences University of Stirling, Stirling, UK.
authors.forename: James
authors.initials: J
authors.lastname: Dick
authors.affiliation: Nutrition Group, Institute of Aquaculture, School of Natural Sciences University of Stirling, Stirling, UK.
authors.forename: J Gordon
authors.initials: JG
authors.lastname: Bell
authors.affiliation: Exercise and Metabolism Research Group, Department of Kinesiology, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Stuart M
authors.initials: SM
authors.lastname: Phillips
authors.affiliation: Health and Exercise Sciences Research Group, School of Sport University of Stirling, Stirling, UK.
authors.forename: Stuart D R
authors.initials: SD
authors.lastname: Galloway
authors.affiliation: Health and Exercise Sciences Research Group, School of Sport University of Stirling, Stirling, UK.
authors.forename: D Lee
authors.initials: DL
authors.lastname: Hamilton
authors.affiliation: Health and Exercise Sciences Research Group, School of Sport University of Stirling, Stirling, UK.
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Tipton
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D045930:Anabolic Agents / Q000008:administration & dosage*; D001706:Biopsy; D004044:Dietary Proteins / Q000008:administration & dosage*; D019587:Dietary Supplements; D005395:Fish Oils / Q000008:administration & dosage*; D006801:Humans; D008297:Male; D009124:Muscle Proteins / Q000096:biosynthesis*; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism; D009210:Myofibrils / Q000187:drug effects* / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D010766:Phosphorylation; D014176:Protein Biosynthesis / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D055070:Resistance Training; D038762:Ribosomal Protein S6 Kinases, 70-kDa / Q000378:metabolism; D012606:Scotland; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D045930:Anabolic Agents; D004044:Dietary Proteins; D005395:Fish Oils; D009124:Muscle Proteins; D010743:Phospholipids; C526181:PRKAA2 protein, human; D051057:Proto-Oncogene Proteins c-akt; D038762:Ribosomal Protein S6 Kinases, 70-kDa; D055372:AMP-Activated Protein Kinases
keywords: Fish oil; myofibrillar muscle protein synthesis; p70S6K1; resistance exercise