title: Physical characteristics and biocompatibility of the polycaprolactone-biphasic calcium phosphate scaffolds fabricated using the modified melt stretching and multilayer deposition.
abstract: Physical properties and biocompatibility of polycaprolactone (PCL)-biphasic calcium phosphate (BCP) scaffolds fabricated by the modified melt stretching and multilayer deposition (mMSMD) technique were evaluated in vitro. The PCL-BCP scaffold specimens included group A; PCL: BCP (wt%) = 80:20 and group B; 70:30. Mechanical properties of the scaffolds were assessed using a universal testing machine. Degradation behaviors of the scaffolds were assessed over 60 days. The amount of calcium and phosphate ions released from the scaffolds was detected over 30 days. Attachment and growth of osteoblasts on the scaffolds and indirect cytocompatibility to those cells were evaluated. The results showed that the scaffolds of both groups could withstand compressive forces on their superior aspect very well; however, their lateral aspect could only withstand light forces. Degradation of the scaffolds over 2 months was low (group A = 1.92 ± 0.47% and group B = 2.9 ± 1.3%,p > 0.05). The concentrations of calcium and phosphate ions released from the scaffolds of both groups significantly increased on day 7 (p < 0.05). Growth of the cells seemed to relate to accumulative increase in those ions. All results between the two ratios of the scaffolds were not statistically different.
authors.affiliation: Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Prince of Songkla University, Hatyai, Songkhla, Thailand nuttawut.t@psu.ac.th.
authors.forename: Nuttawut
authors.initials: N
authors.lastname: Thuaksuban
authors.affiliation: Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Prince of Songkla University, Hatyai, Songkhla, Thailand.
authors.forename: Thunmaruk
authors.initials: T
authors.lastname: Luntheng
authors.affiliation: National Metal and Materials Technology Center, Thailand Science Park, Pathumthani, Thailand.
authors.forename: Naruporn
authors.initials: N
authors.lastname: Monmaturapoj
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D004867:Equipment Design; D006882:Hydroxyapatites / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D055616:Microtechnology / Q000295:instrumentation; D010006:Osteoblasts / Q000166:cytology*; D011091:Polyesters / Q000737:chemistry*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D006882:Hydroxyapatites; D007477:Ions; D011091:Polyesters; C074950:hydroxyapatite-beta tricalcium phosphate; C016240:polycaprolactone
keywords: Scaffold; biphasic calcium phosphate; hydroxyapatite; polycarprolactone; tricalcium phosphate