title: Immunobiotic Bifidobacteria Strains Modulate Rotavirus Immune Response in Porcine Intestinal Epitheliocytes via Pattern Recognition Receptor Signaling.
abstract: In this work, we aimed to characterize the antiviral response of an originally established porcine intestinal epithelial cell line (PIE cells) by evaluating the molecular innate immune response to rotavirus (RVs). In addition, we aimed to select immunomodulatory bacteria with antiviral capabilities. PIE cells were inoculated with RVs isolated from different host species and the infective titers and the molecular innate immune response were evaluated. In addition, the protection against RVs infection and the modulation of immune response by different lactic acid bacteria (LAB) strains was studied. The RVs strains OSU (porcine) and UK (bovine) effectively infected PIE cells. Our results also showed that RVs infection in PIE cells triggered TLR3-, RIG-I- and MDA-5-mediated immune responses with activation of IRF3 and NF-κB, induction of IFN-β and up-regulation of the interferon stimulated genes MxA and RNase L. Among the LAB strains tested, Bifidobacterium infantis MCC12 and B. breve MCC1274 significantly reduced RVs titers in infected PIE cells. The beneficial effects of both bifidobacteria were associated with reduction of A20 expression, and improvements of IRF-3 activation, IFN-β production, and MxA and RNase L expressions. These results indicate the value of PIE cells for studying RVs molecular innate immune response in pigs and for the selection of beneficial bacteria with antiviral capabilities.
authors.affiliation: Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Takamasa
authors.initials: T
authors.lastname: Ishizuka
authors.affiliation: Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.|Livestock Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Paulraj
authors.initials: P
authors.lastname: Kanmani
authors.affiliation: Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.|Livestock Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Kobayashi
authors.affiliation: Viral Diseases and Epidemiology Research Division, National Institute of Animal Health, NARO, Tsukuba, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Miyazaki
authors.affiliation: Research and Development Section, Institute of Animal Health, JA Zen-noh (National Federation of Agricultural Cooperative Associations), Chiba, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Soma
authors.affiliation: Department of Food, Agriculture and Environment, Miyagi University, Sendai, Japan.
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Suda
authors.affiliation: Livestock Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.|Cell Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Aso
authors.affiliation: Cell Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.|Infection Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Nochi
authors.affiliation: Food Science and Technology Institute, Morinaga Milk Industry Co. Ltd, Zama, Kanagawa, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Iwabuchi
authors.affiliation: Food Science and Technology Institute, Morinaga Milk Industry Co. Ltd, Zama, Kanagawa, Japan.
authors.forename: Jin-zhong
authors.initials: JZ
authors.lastname: Xiao
authors.affiliation: Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Tadao
authors.initials: T
authors.lastname: Saito
authors.affiliation: Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.|Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman, Argentina.
authors.forename: Julio
authors.initials: J
authors.lastname: Villena
authors.affiliation: Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.|Livestock Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Haruki
authors.initials: H
authors.lastname: Kitazawa
mesh_terms: D000818:Animals; D001419:Bacteria / Q000378:metabolism; D001644:Bifidobacterium / Q000276:immunology*; D002460:Cell Line; D004847:Epithelial Cells / Q000821:virology*; D007113:Immunity, Innate; D056747:Immunomodulation; D050838:Interferon Regulatory Factor-3 / Q000378:metabolism; D007422:Intestines / Q000473:pathology*; D019344:Lactic Acid; D019936:Probiotics / Q000378:metabolism*; D051192:Receptors, Pattern Recognition / Q000378:metabolism*; D012401:Rotavirus / Q000276:immunology* / Q000472:pathogenicity; D015398:Signal Transduction; D034421:Sus scrofa; D048008:TNF Receptor-Associated Factor 3 / Q000378:metabolism
chemical_list: D050838:Interferon Regulatory Factor-3; D051192:Receptors, Pattern Recognition; D048008:TNF Receptor-Associated Factor 3; D019344:Lactic Acid