title: The Stress-Induced Soybean NAC Transcription Factor GmNAC81 Plays a Positive Role in Developmentally Programmed Leaf Senescence.
abstract: The onset of leaf senescence is a highly regulated developmental change that is controlled by both genetics and the environment. Senescence is triggered by massive transcriptional reprogramming, but functional information about its underlying regulatory mechanisms is limited. In the current investigation, we performed a functional analysis of the soybean (Glycine max) osmotic stress- and endoplasmic reticulum (ER) stress-induced NAC transcription factor GmNAC81 during natural leaf senescence using overexpression studies and reverse genetics. GmNAC81-overexpressing lines displayed accelerated flowering and leaf senescence but otherwise developed normally. The precocious leaf senescence of GmNAC81-overexpressing lines was associated with greater Chl loss, faster photosynthetic decay and higher expression of hydrolytic enzyme-encoding GmNAC81 target genes, including the vacuolar processing enzyme (VPE), an executioner of vacuole-triggered programmed cell death (PCD). Conversely, virus-induced gene silencing-mediated silencing of GmNAC81 delayed leaf senescence and was associated with reductions in Chl loss, lipid peroxidation and the expression of GmNAC81 direct targets. Promoter-reporter studies revealed that the expression pattern of GmNAC81 was associated with senescence in soybean leaves. Our data indicate that GmNAC81 is a positive regulator of age-dependent senescence and may integrate osmotic stress- and ER stress-induced PCD responses with natural leaf senescence through the GmNAC81/VPE regulatory circuit.
authors.affiliation: National Institute of Science and Technology in Plant-Pest Interactions, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil.
authors.forename: Maiana Reis
authors.initials: MR
authors.lastname: Pimenta
authors.affiliation: National Institute of Science and Technology in Plant-Pest Interactions, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil.
authors.forename: Priscila Alves
authors.initials: PA
authors.lastname: Silva
authors.affiliation: National Institute of Science and Technology in Plant-Pest Interactions, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil.
authors.forename: Giselle Camargo
authors.initials: GC
authors.lastname: Mendes
authors.affiliation: National Institute of Science and Technology in Plant-Pest Interactions, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil Departamento de Bioquímica e Biologia Molecular/Bioagro, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil.
authors.forename: Janaína Roberta
authors.initials: JR
authors.lastname: Alves
authors.affiliation: National Institute of Science and Technology in Plant-Pest Interactions, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil Departamento de Bioquímica e Biologia Molecular/Bioagro, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil.
authors.forename: Hanna Durso Neves
authors.initials: HD
authors.lastname: Caetano
authors.affiliation: National Institute of Science and Technology in Plant-Pest Interactions, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil Departamento de Bioquímica e Biologia Molecular/Bioagro, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil.
authors.forename: Joao Paulo Batista
authors.initials: JP
authors.lastname: Machado
authors.affiliation: National Institute of Science and Technology in Plant-Pest Interactions, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil Departamento de Bioquímica e Biologia Molecular/Bioagro, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil.
authors.forename: Otavio José Bernardes
authors.initials: OJ
authors.lastname: Brustolini
authors.affiliation: National Institute of Science and Technology in Plant-Pest Interactions, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil Departamento de Bioquímica e Biologia Molecular/Bioagro, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil.
authors.forename: Paola Avelar
authors.initials: PA
authors.lastname: Carpinetti
authors.affiliation: National Institute of Science and Technology in Plant-Pest Interactions, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil Departamento de Bioquímica e Biologia Molecular/Bioagro, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil.
authors.forename: Bruno Paes
authors.initials: BP
authors.lastname: Melo
authors.affiliation: National Institute of Science and Technology in Plant-Pest Interactions, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil Departamento de Bioquímica e Biologia Molecular/Bioagro, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil.
authors.forename: José Cleydson Ferreira
authors.initials: JC
authors.lastname: Silva
authors.affiliation: National Institute of Science and Technology in Plant-Pest Interactions, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil Departamento de Bioquímica e Biologia Molecular/Bioagro, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil.
authors.forename: Gustavo Leão
authors.initials: GL
authors.lastname: Rosado
authors.affiliation: National Institute of Science and Technology in Plant-Pest Interactions, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil Departamento de Biologia, Universidade Federal do Espírito Santo, 29500.000, Alegre, ES, Brazil.
authors.forename: Márcia Flores Silva
authors.initials: MF
authors.lastname: Ferreira
authors.affiliation: National Institute of Science and Technology in Plant-Pest Interactions, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil Departamento de Bioquímica e Biologia Molecular/Bioagro, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil.
authors.forename: Maximillir
authors.initials: M
authors.lastname: Dal-Bianco
authors.affiliation: Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil.
authors.forename: Edgard Augusto de Toledo
authors.initials: EA
authors.lastname: Picoli
authors.affiliation: Embrapa Recursos Genéticos e Biotecnologia, PqEB W5 Norte, 70770-900, Brasília, DF, Brazil.
authors.forename: Francisco José Lima
authors.initials: FJ
authors.lastname: Aragao
authors.affiliation: National Institute of Science and Technology in Plant-Pest Interactions, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil Departamento de Bioquímica e Biologia Molecular/Bioagro, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil.
authors.forename: Humberto Josué Oliveira
authors.initials: HJ
authors.lastname: Ramos
authors.affiliation: National Institute of Science and Technology in Plant-Pest Interactions, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil Departamento de Bioquímica e Biologia Molecular/Bioagro, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil bbfontes@ufv.br.
authors.forename: Elizabeth Pacheco Batista
authors.initials: EP
authors.lastname: Fontes
mesh_terms: D000818:Animals; D017209:Apoptosis; D016922:Cellular Senescence; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism; D059865:Endoplasmic Reticulum Stress; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D009997:Osmotic Pressure; D018515:Plant Leaves / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D013025:Glycine max / Q000235:genetics / Q000502:physiology*; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors; D003546:Cysteine Endopeptidases; C089278:vacuolar processing enzyme
keywords: Developmental regulation; Glycine max; GmNAC81; Leaf senescence; NAC family; Transcription factor