title: Effects of the Oral Nutritional Supplement Containing Arginine, Glutamine, and Hydroxymethylbutyrate (Abound®) on Healing of Colonic Anastomoses in Rats.
abstract: We evaluated the effects of the oral nutritional supplement containing arginine, glutamine, and hydroxymethylbutyrate (Abound®) on healing of colonic anastomoses in experimental rat model. Seventy Wistar-Albino male rats were divided into seven groups. Colon transection and anastomosis were performed in all groups except for the sham group. In groups 2 and 5, rats were fed with standard rat chow after the operation. Oral nutritional supplement was added to standard nutrition for 3 days postoperatively in group 3 and 7 days in group 6 and preoperative 7 days plus postoperative 3 days in group 4 and preoperative 7 days plus postoperative 7 days in group 7. Bursting pressures were measured, adhesions were evaluated, and tissue samples were taken for measurement of tissue hydroxyproline levels and for histopathological examination. The usage of oral nutritional supplement had positive effects on bursting pressures, tissue hydroxyproline levels, and histopathological findings of anastomoses, but feeding with oral nutritional supplement both preoperatively and postoperatively had no additive effect on these parameters when compared with the groups that were fed only postoperatively. The mixture of arginine, glutamine, and hydroxymethylbutyrate may be safely used for achieving better healing results after colonic anastomoses.
authors.affiliation: Ankara Training and Research Hospital General Surgery Department, Ankara, Turkey.
authors.forename: Rifat
authors.initials: R
authors.lastname: Kusabbi
authors.affiliation: Ankara Training and Research Hospital General Surgery Department, Ankara, Turkey.
authors.forename: Kemal
authors.initials: K
authors.lastname: Kismet
authors.affiliation: Ankara Training and Research Hospital General Surgery Department, Ankara, Turkey.
authors.forename: Serdar
authors.initials: S
authors.lastname: Kuru
authors.affiliation: Ankara Training and Research Hospital General Surgery Department, Ankara, Turkey.
authors.forename: Aziz Mutlu
authors.initials: AM
authors.lastname: Barlas
authors.affiliation: Ankara Training and Research Hospital General Surgery Department, Ankara, Turkey.
authors.forename: Mehmet Esat
authors.initials: ME
authors.lastname: Duymus
authors.affiliation: Ankara Training and Research Hospital General Surgery Department, Ankara, Turkey.
authors.forename: Adnan
authors.initials: A
authors.lastname: Hasanoglu
authors.affiliation: Ankara Training and Research Hospital Biochemistry Department, Ankara, Turkey.
authors.forename: Elmas
authors.initials: E
authors.lastname: Ogus
authors.affiliation: Ankara Training and Research Hospital Biochemistry Department, Ankara, Turkey.
authors.forename: Hatice
authors.initials: H
authors.lastname: Surer
authors.affiliation: Ankara Training and Research Hospital Pathology Department, Ankara, Turkey.
authors.forename: Huseyin
authors.initials: H
authors.lastname: Ustun
authors.affiliation: Ankara Training and Research Hospital General Surgery Department, Ankara, Turkey.
authors.forename: Osman
authors.initials: O
authors.lastname: Guler
keywords: Arginine; Colonic anastomosis; Experimental rat model; Glutamine; Hydroxymethylbutyrate