title: Protection by polyphenol extract from olive stones against apoptosis produced by oxidative stress in human neuroblastoma cells
abstract: We evaluated the protective activity of an extract from a by-product such as olive stones, through its ability to inhibit H202 induced apoptosis in the SH-SY5Y human neuroblastoma cell line.
  
  
  
  To such end, 20,000 cells/well were cultivated and differentiation with retinoic acid was initiated. Once the cells were differentiated, apoptosis was induced with and without H2O2 extract. Finally, cDNA extraction was performed, and pro-apoptotic genes Bax and anti-apoptotic genes Bcl-2 were analyzed. Quantification of the gene expression was performed using the GAPDH gene marker.
  
  
  
  Cell viability with the extract is 97.6% (SD 5.7) with 10 mg/l and 62.8% (SD 1.2) to 50 mg/l, using 10 mg/l for the biomarker assay. The retinoic acid differentiated SH-S cell line (10 μM) shows a clear apoptosis when treated with H2O2 150 μM, with a Bax/Bcl-2 ratio of 3.75 (SD 0.80) in contrast to the differentiated control cells subjected to H2O2 and with extract, which have the same ratio of 1.02 (SD 0.01-0.03).
  
  
  
  The olive stone extract shows anti-apoptotic activity in the provoked cell death of SH-SY5Y human neuroblastoma cells in their normal state, defending them from oxidative stress which produces a significant increase in the apoptotic gene ratio in contrast to anti-apoptotic genes (Bax/Bcl-2).
authors.affiliation: Department of Pharmacology, Pediatrics and Organic Chemistry. ernestocort@gmail.com.
authors.forename: Ernesto
authors.initials: E
authors.lastname: Cortés-Castell
authors.forename: Carmen
authors.initials: C
authors.lastname: Veciana-Galindo
authors.forename: Luis
authors.initials: L
authors.lastname: Torró-Montell
authors.forename: Antonio
authors.initials: A
authors.lastname: Palazón-Bru
authors.forename: Elia
authors.initials: E
authors.lastname: Sirvent-Segura
authors.forename: Vicente
authors.initials: V
authors.lastname: Gil-Guillén
authors.forename: Mercedes
authors.initials: M
authors.lastname: Rizo-Baeza
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D006801:Humans; D009447:Neuroblastoma / Q000473:pathology*; D018696:Neuroprotective Agents / Q000494:pharmacology; D031658:Olea / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D059808:Polyphenols / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D051017:Apoptosis Regulatory Proteins; D018696:Neuroprotective Agents; D010936:Plant Extracts; D059808:Polyphenols
keywords: Apoptosis; Human neuroblastome cells; Olive stones; Polyphenols