title: Preparation of photonic-magnetic responsive molecularly imprinted microspheres and their application to fast and selective extraction of 17β-estradiol.
abstract: Photonic-magnetic responsive molecularly imprinted microspheres (PM-MIMs) were prepared by seed polymerization, through suitable functionalization of magnetic nanoparticles for further coating with photoresponsive functional monomer and imprinted layers, and then were successfully applied to the fast and selective extraction of 17β-estradiol (17β-E2) from real samples. The PM-MIMs possessed a sandwich micro-spherical structure containing Fe3O4 core, SiO2 middle layer, and MIPs shell with thickness of 25 nm. The PM-MIMs displayed excellent photoresponsive properties and could be rapidly separated from solutions under an external magnet. The PM-MIMs had specific affinity towards 17β-E2 with high adsorption capacity (Qmax=0.84 mg g(-1)) and fast binding kinetics (Kd=26.08 mg L(-1)). The PM-MIMs proved to be an ideal photoswitch with the ability of reversible uptake and release of 17β-E2 upon alternate 365 and 440 nm irradiation: 45.0% of 17β-E2 released from the PM-MIMs upon 365 nm irradiation, and 94.0% of the released 17β-E2 was rebound to the PM-MIMs at 440 nm. Accordingly, the PM-MIMs were applied for fast separation and extraction of 17β-E2 followed by HPLC-UV determination, presenting the low limit of detection (LOD, S/N=3) and quantification (LOQ, S/N=10) of 0.18 and 0.62 μmol L(-1), respectively. The high recoveries for spiked milk powder and drinking water samples were in the range of 97.5-113.0% with relative standard deviations less than 4.4%. This study reasonably combined photonic response, magnetic separation and surface imprinting, which endowed the PM-MIMs with significant advantages of high adsorption capacity and fast binding kinetics, convenient separation and recycled use, and simple rapid eco-benign adsorption/elution processes for template molecules. Thus, the PM-MIMs based method may be a simple, rapid, convenient, cost-effective and environmentally-friendly way for simultaneous separation, enrichment and detection of trace 17β-E2 in complicated matrices.
authors.affiliation: School of Resources, Environmental, and Chemical Engineering, Nanchang University, Nanchang 330031, China; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Hailong
authors.initials: H
authors.lastname: Peng
authors.affiliation: School of Resources, Environmental, and Chemical Engineering, Nanchang University, Nanchang 330031, China; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address: huaxiong100@126.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Xiong
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Ningxiang
authors.initials: N
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Fangjian
authors.initials: F
authors.lastname: Ning
authors.affiliation: School of Resources, Environmental, and Chemical Engineering, Nanchang University, Nanchang 330031, China.
authors.forename: Jieping
authors.initials: J
authors.lastname: Fan
authors.affiliation: School of Resources, Environmental, and Chemical Engineering, Nanchang University, Nanchang 330031, China.
authors.forename: Zheling
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China. Electronic address: lxchen@yic.ac.cn.
authors.forename: Lingxin
authors.initials: L
authors.lastname: Chen
mesh_terms: D000327:Adsorption; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004958:Estradiol / Q000737:chemistry / Q000302:isolation & purification*; D008280:Magnetics; D008863:Microspheres; D054802:Molecular Imprinting; D053758:Nanoparticles / Q000737:chemistry; D058105:Polymerization; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D004958:Estradiol; D012822:Silicon Dioxide
keywords: 17β-Estradiol; Magnetic separation; Molecularly imprinted polymers; Photonic responsive