title: Concomitant biohydrogen and poly-β-hydroxybutyrate production from dark fermentation effluents by adapted Rhodobacter sphaeroides and mixed photofermentative cultures.
abstract: This work aimed at investigating concomitant production of biohydrogen and poly-β-hydroxybutyrate (PHB) by photofermentation (PF) using dark fermentation effluents (DFE). An adapted culture of Rhodobacter sphaeroides AV1b (pH 6.5, 24±2°C) achieved H2 and PHB yields of 256 (±2) NmLH2/g Chemical Oxygen Demand (COD) and 273.8mgPHB/gCOD (32.5±3% of the dry cells weight (DCW)), respectively. When a diluted (1:2) DFE medium was applied to the adapted pure and mixed photofermentative culture, the respective H2 yields were 164.0 (±12) and 71.3 (±6) NmLH2/gCOD and the PHB yields were 212.1 (±105.2) and 50.7 (±2.7) mgPHB/gCOD added, corresponding to 24 (±0.7) and 6.3 (±0) % DCW, respectively. The concomitant H2 and PHB production from the PF process gave a good DFE post treatment achieving up to 80% COD removal from the initial DFE.
authors.affiliation: Department of Civil and Mechanical Engineering, University of Cassino and Southern Lazio, via Di Biasio 43, 03043 Cassino (FR), Italy; Department of Civil, Architectural and Environmental Engineering, University of Naples Federico II, via Claudio 21, 80125 Naples, Italy. Electronic address: a.ghimire@unicas.it.
authors.forename: Anish
authors.initials: A
authors.lastname: Ghimire
authors.affiliation: Department of Civil, Architectural and Environmental Engineering, University of Naples Federico II, via Claudio 21, 80125 Naples, Italy.
authors.forename: Serena
authors.initials: S
authors.lastname: Valentino
authors.affiliation: Department of Mathematics and Applications Renato Caccioppoli, University of Naples Federico II, via Cintia, Monte S. Angelo, I-80126 Naples, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Frunzo
authors.affiliation: Department of Civil, Architectural and Environmental Engineering, University of Naples Federico II, via Claudio 21, 80125 Naples, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Pirozzi
authors.affiliation: UNESCO-IHE Institute for Water Education, Westvest 7, 2611 AX Delft, The Netherlands.
authors.forename: Piet N L
authors.initials: PN
authors.lastname: Lens
authors.affiliation: Department of Civil and Mechanical Engineering, University of Cassino and Southern Lazio, via Di Biasio 43, 03043 Cassino (FR), Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Esposito
mesh_terms: D056804:Biofuels; D019149:Bioreactors / Q000382:microbiology*; D005285:Fermentation; D006859:Hydrogen / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000378:metabolism*; D011091:Polyesters / Q000378:metabolism*; D012242:Rhodobacter sphaeroides / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
chemical_list: D056804:Biofuels; D006859:Hydrogen; D006885:Hydroxybutyrates; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
keywords: Biohydrogen; Dark fermentation; Photofermentation; Poly-β-hydroxybutyrate