title: Effect of polyclonal activators on cytokine production by blood cells and by malignant breast cancer cells.
abstract: The production of cytokines by peripheral blood cells and biopsy specimens of tumors stimulated by polyclonal activators (PAs) was evaluated in 34 patients with invasive ductal breast carcinoma using enzyme-linked immunosorbent assay (ELISA). Positive correlation between the stimulation index of polyclonal activators (SIPA) for IL-18 production by the tumor and the relative content of poorly differentiated cells was revealed. The latter, in turn, was positively correlated with the numbers of normal and pathologic mitoses and the degree of malignancy. Cancer cells can produce IL-18, which is involved in the process of angiogenesis, stimulates invasion and metastasis. Decrease in SIPA for the production of IL-6 and GCSF by peripheral blood cells could serve as an indicator of malignant progression in invasive ductal breast carcinoma.
authors.affiliation: Research Center for Molecular Biology and Biophysics, Siberian Branch, Russian Academy of Medical Sciences, Novosibirsk, Russia. tkunts@ngs.ru.
authors.forename: T A
authors.initials: TA
authors.lastname: Kunts
authors.affiliation: Novosibirsk State Medical University, Ministry of Health, Novosibirsk, Russia.
authors.forename: K V
authors.initials: KV
authors.lastname: Karpukhina
authors.affiliation: Novosibirsk State Medical University, Ministry of Health, Novosibirsk, Russia.
authors.forename: E S
authors.initials: ES
authors.lastname: Mikhaylova
authors.affiliation: Research Center for Molecular Biology and Biophysics, Siberian Branch, Russian Academy of Medical Sciences, Novosibirsk, Russia.
authors.forename: I O
authors.initials: IO
authors.lastname: Marinkin
authors.affiliation: ZAO Vector-Best, Kol'tsovo, Novosibirsk oblast, Russia.
authors.forename: N A
authors.initials: NA
authors.lastname: Varaksin
authors.affiliation: Novosibirsk State Medical University, Ministry of Health, Novosibirsk, Russia.
authors.forename: A I
authors.initials: AI
authors.lastname: Autenshlyus
authors.affiliation: Novosibirsk State Medical University, Ministry of Health, Novosibirsk, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Lyakhovich
mesh_terms: D001773:Blood Cells / Q000378:metabolism* / Q000473:pathology; D063646:Carcinogenesis / Q000235:genetics; D018270:Carcinoma, Ductal, Breast / Q000097:blood* / Q000235:genetics / Q000473:pathology; D003208:Concanavalin A / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood / Q000235:genetics*; D006801:Humans; D020382:Interleukin-18 / Q000097:blood / Q000235:genetics*; D015850:Interleukin-6 / Q000097:blood / Q000235:genetics*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008938:Mitosis / Q000235:genetics; D009361:Neoplasm Invasiveness / Q000235:genetics; D009362:Neoplasm Metastasis; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000473:pathology; D010835:Phytohemagglutinins / Q000494:pharmacology
chemical_list: D020382:Interleukin-18; D015850:Interleukin-6; D008070:Lipopolysaccharides; D010835:Phytohemagglutinins; D003208:Concanavalin A; D016179:Granulocyte Colony-Stimulating Factor