title: MicroRNA-26a/b and their host genes synergistically regulate triacylglycerol synthesis by targeting the INSIG1 gene.
abstract: The microRNA-26 (miR-26) family is known to control adipogenesis in non-ruminants. The genomic loci of miR-26a and miR-26b have been localized in the introns of genes encoding for the proteins of the C-terminal domain RNA polymerase II polypeptide A small phosphatase (CTDSP) family. Insulin-induced gene 1 (INSIG1) encodes a protein with a key role in the regulation of lipogenesis in rodent liver. In the present study, we investigated the synergistic function of the miR-26 family and their host genes in goat mammary epithelial cells (GMEC). Downregulation of miR-26a/b and their host genes in GMEC decreased the expression of genes relate to fatty acid synthesis (PPARG, LXRA, SREBF1, FASN, ACACA, GPAM, LPIN1, DGAT1 and SCD1), triacylglycerol accumulation and unsaturated fatty acid synthesis. Luciferase reporter assays confirmed INSIG1 as a direct target of miR-26a/b. Furthermore, inhibition of the CTDSP family also downregulated the expression of INSIG1. Taken together, our findings highlight a functional association of miR-26a/b, their host genes and INSIG1, and provide new insights into the regulatory network controlling milk fat synthesis in GMEC. The data indicate that targeting this network via nutrition might be important for regulating milk fat synthesis in ruminants.
authors.affiliation: a Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University , Yangling , Shaanxi , PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: a Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University , Yangling , Shaanxi , PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Luo
authors.affiliation: a Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University , Yangling , Shaanxi , PR China.
authors.forename: Tianying
authors.initials: T
authors.lastname: Zhang
authors.affiliation: a Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University , Yangling , Shaanxi , PR China.
authors.forename: Huibin
authors.initials: H
authors.lastname: Tian
authors.affiliation: a Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University , Yangling , Shaanxi , PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Ma
authors.affiliation: a Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University , Yangling , Shaanxi , PR China.
authors.forename: Huifen
authors.initials: H
authors.lastname: Xu
authors.affiliation: a Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University , Yangling , Shaanxi , PR China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Yao
authors.affiliation: b Mammalian NutriPhysioGenomics , Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois , Urbana , USA.
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Loor
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D006041:Goats; D008321:Mammary Glands, Animal / Q000166:cytology* / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D035683:MicroRNAs; D014280:Triglycerides
keywords: CTDSP family; INSIG1; TAG accumulation; microRNA-26