title: Marsdenia tenacissima extract suppresses A549 cell migration through regulation of CCR5-CCL5 axis, Rho C, and phosphorylated FAK.
abstract: Marsdenia tenacissima, a traditional Chinese medicine, is long been used to treat various diseases including asthma, cancer, trachitis, tonsillitis, pharyngitis, cystitis, and pneumonia. Although Marsdenia tenacissima has been demonstrated to have strong anti-tumor effects against primary tumors, its effect on cancer metastasis remains to be defined, and the molecular mechanism underlying the anti-metastatic effect is unknown. In the present study, we investigated the effects of XAP (an extract of Marsdenia tenacissima) on A549 lung cancer cell migration and explored the role of CCR5-CCL5 axis in the anti-metastatic effects of XAP. Our resutls showed that XAP inhibited A549 lung cancer cell migration and invasion in a dose-dependent manner. The protein levels of CCR5, but not CCR9 and CXCR4, were decreased by XAP. The secretion of CCL5, the ligand of CCR5, was reduced by XAP. XAP down-regulated Rho C expression and FAK phosphorylation. In conclusion, XAP inhibited A549 cell migration and invasion through down-regulation of CCR5-CCL5 axis, Rho C, and FAK.
authors.affiliation: New Drug Screen Center, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Sen-Sen
authors.initials: SS
authors.lastname: Lin
authors.affiliation: New Drug Screen Center, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Fang-Fang
authors.initials: FF
authors.lastname: Li
authors.affiliation: New Drug Screen Center, China Pharmaceutical University, Nanjing 210009, China. Electronic address: cpusunli@126.com.
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.affiliation: New Drug Screen Center, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Fan
authors.affiliation: New Drug Screen Center, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Gu
authors.affiliation: New Drug Screen Center, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Lu-Yong
authors.initials: LY
authors.lastname: Zhang
authors.affiliation: Nanjing Sanhome Pharmaceutical Co., Ltd., Nanjing, 210046, China.
authors.forename: Song
authors.initials: S
authors.lastname: Qin
authors.affiliation: New Drug Screen Center, China Pharmaceutical University, Nanjing 210009, China. Electronic address: yuanst@aliyun.com.
authors.forename: Sheng-Tao
authors.initials: ST
authors.lastname: Yuan
mesh_terms: D000072283:A549 Cells; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D018946:Chemokine CCL5 / Q000378:metabolism*; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms; D031101:Marsdenia / Q000737:chemistry*; D010766:Phosphorylation; D010936:Plant Extracts / Q000494:pharmacology*; D019713:Receptors, CCR5 / Q000378:metabolism*; D020741:rho GTP-Binding Proteins / Q000378:metabolism; D000075024:rhoC GTP-Binding Protein
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D018946:Chemokine CCL5; D010936:Plant Extracts; D019713:Receptors, CCR5; D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human; C066690:RHOC protein, human; D020741:rho GTP-Binding Proteins; D000075024:rhoC GTP-Binding Protein
keywords: A549 cell migration; CCR5-CCL5 axis; FAK; Rho C; XAP