title: Ploidy Level and DNA Content of Erianthus arundinaceus as Determined by Flow Cytometry and the Association with Biological Characteristics.
abstract: Erianthus arundinaceus is not only an important germplasm resource for sugarcane breeding but also a potential bioenergy plant. Making clear the distribution of the chromosome ploidy of wild E. arundinaceus in china is the premise of the research and utilization of this species. Therefore, the objectives of this study were to determine the ploidy level and DNA content of the 55 E. arundinaceus accessions using flow cytometry and to identify the correlation between ploidy and phenotypic traits. Among the 55 accessions, four tetraploids and 51 hexaploids were identified. The four tetraploids originated from Mengma Yunnan, Shuangjiang Yunnan, Gaozhou Guangdong and Chengle Sichuan. The mean DNA content was 4.82 pg/2C for the tetraploid and 7.30 pg/2C for the hexaploid plants. The ploidy was negatively correlated with cellulose content and positively correlated (P<0.05) with plant height, stem diameter, leaf width, dry weight per plant, fresh weight per plant and hemicellulose content. However, ploidy was not correlated with leaf length, tiller number and the ratio of dry weight and fresh weight. This study will be useful for revealing the distribution of the ploidy of wild E. arundinaceus in Chin, traits markers analysis, and utilization of this species, such as cultivar improvement and sugarcane breeding in the future.
authors.affiliation: Sichuan Academy of Grassland Sciences, Chengdu, Sichuan, China.
authors.forename: Jiajun
authors.initials: J
authors.lastname: Yan
authors.affiliation: Sichuan Academy of Grassland Sciences, Chengdu, Sichuan, China.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Sichuan Academy of Grassland Sciences, Chengdu, Sichuan, China.|Department of Grassland Science, Animal Science and Technology College, Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Kaiyan
authors.initials: K
authors.lastname: Sun
authors.affiliation: Sichuan Academy of Grassland Sciences, Chengdu, Sichuan, China.|Department of Grassland Science, Animal Science and Technology College,Sichuan Agricultural University, Ya'an, Sichuan, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Chang
authors.affiliation: Sichuan Academy of Grassland Sciences, Chengdu, Sichuan, China.
authors.forename: Shiqie
authors.initials: S
authors.lastname: Bai
authors.affiliation: Department of Grassland Science, Animal Science and Technology College, Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Yixin
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Grassland Science, Animal Science and Technology College,Sichuan Agricultural University, Ya'an, Sichuan, China.
authors.forename: Linkai
authors.initials: L
authors.lastname: Huang
authors.affiliation: Sichuan Academy of Grassland Sciences, Chengdu, Sichuan, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Sichuan Academy of Grassland Sciences, Chengdu, Sichuan, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Grassland Science, Animal Science and Technology College,Sichuan Agricultural University, Ya'an, Sichuan, China.
authors.forename: Yanhai
authors.initials: Y
authors.lastname: Dong
mesh_terms: D002681:China; D018744:DNA, Plant / Q000235:genetics*; D005434:Flow Cytometry; D010641:Phenotype; D000069600:Plant Breeding; D011003:Ploidies; D006109:Poaceae / Q000235:genetics* / Q000254:growth & development; D031786:Saccharum / Q000235:genetics / Q000254:growth & development
chemical_list: D018744:DNA, Plant