title: Identification of Deleterious Mutations in Myostatin Gene of Rohu Carp (Labeo rohita) Using Modeling and Molecular Dynamic Simulation Approaches.
abstract: The myostatin (MSTN) is a known negative growth regulator of skeletal muscle. The mutated myostatin showed a double-muscular phenotype having a positive significance for the farmed animals. Consequently, adequate information is not available in the teleosts, including farmed rohu carp, Labeo rohita. In the absence of experimental evidence, computational algorithms were utilized in predicting the impact of point mutation of rohu myostatin, especially its structural and functional relationships. The four mutations were generated at different positions (p.D76A, p.Q204P, p.C312Y, and p.D313A) of MSTN protein of rohu. The impacts of each mutant were analyzed using SIFT, I-Mutant 2.0, PANTHER, and PROVEAN, wherein two substitutions (p.D76A and p.Q204P) were predicted as deleterious. The comparative structural analysis of each mutant protein with the native was explored using 3D modeling as well as molecular-dynamic simulation techniques. The simulation showed altered dynamic behaviors concerning RMSD and RMSF, for either p.D76A or p.Q204P substitution, when compared with the native counterpart. Interestingly, incorporated two mutations imposed a significant negative impact on protein structure and stability. The present study provided the first-hand information in identifying possible amino acids, where mutations could be incorporated into MSTN gene of rohu carp including other carps for undertaking further in vivo studies.
authors.affiliation: Fish Genetics and Biotechnology Division, Central Institute of Freshwater Aquaculture (ICAR), Bhubaneswar, Odisha 751 002, India.
authors.forename: Kiran Dashrath
authors.initials: KD
authors.lastname: Rasal
authors.affiliation: Fish Genetics and Biotechnology Division, Central Institute of Freshwater Aquaculture (ICAR), Bhubaneswar, Odisha 751 002, India.
authors.forename: Vemulawada
authors.initials: V
authors.lastname: Chakrapani
authors.affiliation: Fish Genetics and Biotechnology Division, Central Institute of Freshwater Aquaculture (ICAR), Bhubaneswar, Odisha 751 002, India.
authors.forename: Swagat Kumar
authors.initials: SK
authors.lastname: Patra
authors.affiliation: Fish Genetics and Biotechnology Division, Central Institute of Freshwater Aquaculture (ICAR), Bhubaneswar, Odisha 751 002, India.
authors.forename: Shibani D
authors.initials: SD
authors.lastname: Mohapatra
authors.affiliation: Fish Genetics and Biotechnology Division, Central Institute of Freshwater Aquaculture (ICAR), Bhubaneswar, Odisha 751 002, India.
authors.forename: Swapnarani
authors.initials: S
authors.lastname: Nayak
authors.affiliation: Fish Genetics and Biotechnology Division, Central Institute of Freshwater Aquaculture (ICAR), Bhubaneswar, Odisha 751 002, India.
authors.forename: Sasmita
authors.initials: S
authors.lastname: Jena
authors.affiliation: Fish Genetics and Biotechnology Division, Central Institute of Freshwater Aquaculture (ICAR), Bhubaneswar, Odisha 751 002, India.
authors.forename: Jitendra Kumar
authors.initials: JK
authors.lastname: Sundaray
authors.affiliation: Fish Genetics and Biotechnology Division, Central Institute of Freshwater Aquaculture (ICAR), Bhubaneswar, Odisha 751 002, India.
authors.forename: Pallipuram
authors.initials: P
authors.lastname: Jayasankar
authors.affiliation: Fish Genetics and Biotechnology Division, Central Institute of Freshwater Aquaculture (ICAR), Bhubaneswar, Odisha 751 002, India.
authors.forename: Hirak Kumar
authors.initials: HK
authors.lastname: Barman
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D003531:Cypriniformes / Q000235:genetics*; D029941:Fish Proteins / Q000235:genetics*; D020125:Mutation, Missense; D055435:Myostatin / Q000235:genetics*
chemical_list: D029941:Fish Proteins; D055435:Myostatin