title: Mycoplasma bovis isolates recovered from cattle and bison (Bison bison) show differential in vitro effects on PBMC proliferation, alveolar macrophage apoptosis and invasion of epithelial and immune cells.
abstract: In the last few years, several outbreaks of pneumonia, systemically disseminated infection, and high mortality associated with Mycoplasma bovis (M. bovis) in North American bison (Bison bison) have been reported in Alberta, Manitoba, Saskatchewan, Nebraska, New Mexico, Montana, North Dakota, and Kansas. M. bovis causes Chronic Pneumonia and Polyarthritis Syndrome (CPPS) in young, stressed calves in intensively-managed feedlots. M. bovis is not classified as a primary pathogen in cattle, but in bison it appears to be a primary causative agent with rapid progression of disease with fatal outcomes and an average 20% mature herd mortality. Thus, there is a possibility that M. bovis isolates from cattle and bison differ in their pathogenicity. Hence, we decided to compare selected cattle isolates to several bison isolates obtained from clinical cases. We show differences in modulation of PBMC proliferation, invasion of trachea and lung epithelial cells, along with modulation of apoptosis and survival in alveolar macrophages. We concluded that some bison isolates showed less inhibition of cattle and bison PBMC proliferation, were not able to suppress alveolar macrophage apoptosis as efficiently as cattle isolates, and were more or less invasive than the cattle isolate in various cells. These findings provide evidence about the differential properties of M. bovis isolated from the two species and has helped in the selection of bison isolates for genomic sequencing.
authors.affiliation: Vaccine and Infectious Disease Organization-International Vaccine Centre, University of Saskatchewan, 120 Veterinary Rd, Saskatoon S7 N 5E3, SK, Canada. Electronic address: mus537@mail.usask.ca.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Suleman
authors.affiliation: Vaccine and Infectious Disease Organization-International Vaccine Centre, University of Saskatchewan, 120 Veterinary Rd, Saskatoon S7 N 5E3, SK, Canada.
authors.forename: Tracy
authors.initials: T
authors.lastname: Prysliak
authors.affiliation: Vaccine and Infectious Disease Organization-International Vaccine Centre, University of Saskatchewan, 120 Veterinary Rd, Saskatoon S7 N 5E3, SK, Canada.
authors.forename: Kyle
authors.initials: K
authors.lastname: Clarke
authors.affiliation: Bluffton Veterinary Services, Box14, Main St. #2, Bluffton T0C 0M0, AB, Canada.
authors.forename: Pat
authors.initials: P
authors.lastname: Burrage
authors.affiliation: Department of Production Animal Health, University of Calgary Faculty of Veterinary Medicine, 3330 Hospital Drive NW, Calgary T2 N 4N1, AB, Canada.
authors.forename: Claire
authors.initials: C
authors.lastname: Windeyer
authors.affiliation: Vaccine and Infectious Disease Organization-International Vaccine Centre, University of Saskatchewan, 120 Veterinary Rd, Saskatoon S7 N 5E3, SK, Canada.
authors.forename: Jose
authors.initials: J
authors.lastname: Perez-Casal
mesh_terms: D000818:Animals; D017209:Apoptosis; D016164:Bison; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology* / Q000503:physiopathology; D049109:Cell Proliferation; D004847:Epithelial Cells / Q000382:microbiology; D007963:Leukocytes, Mononuclear / Q000382:microbiology*; D016676:Macrophages, Alveolar / Q000382:microbiology*; D050296:Microbial Viability; D009175:Mycoplasma Infections / Q000382:microbiology*; D045682:Mycoplasma bovis / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*
keywords: Apoptosis; Bison; Cattle; Host-pathogen interaction; Mycoplasma bovis; PBMCs