title: Expression of IL-1β, IL-2, IL-10, TNF-β and GM-CSF in peripheral blood leukocytes of rabbits experimentally infected with rabbit haemorrhagic disease virus.
abstract: Rabbit haemorrhagic disease (RHD) is a highly morbid and mortal viral infection of European rabbits. This disease is one of the main causes of death in wild rabbits, and results in large economic losses in farms of rabbits worldwide. Although the first outbreak of this disease was noted in 1984, the pathogenesis of RHD and mechanisms of RHDV (rabbit haemorrhagic disease virus) pathogenecity have still not been fully elucidated. Recent studies indicate a role of the immune response, especially peripheral blood leukocytes (PBL), in the pathogenesis of this disease. Thus, in the present study we investigated the expression of IL-1β, IL-2, IL-10, TNF-β and GM-CSF genes in PBL of RHDV-infected rabbits. We also compared the expression of genes encoding these cytokines in rabbits with different course of RHDV infection (in animals that died 36h postinfection or survived until 60th h after infection). The study revealed that three (IL-10, TNF-β and GM-CSF) out of five investigated genes encoding cytokines showed increased expression in PBL of RHDV-infected rabbits, and the level of expression depended on the course of RHD. The results indicate the potential role of these cytokines in RHDV infection and their influence on the survival time of infected rabbits.
authors.affiliation: Department of Immunology, Faculty of Biology, University of Szczecin, Poland; Molecular Biology and Biotechnology Center, Faculty of Biology, University of Szczecin, Poland. Electronic address: alicja.ryczek@gmail.com.
authors.forename: Alicja
authors.initials: A
authors.lastname: Trzeciak-Ryczek
authors.affiliation: Department of Immunology, Faculty of Biology, University of Szczecin, Poland; Molecular Biology and Biotechnology Center, Faculty of Biology, University of Szczecin, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Tokarz-Deptuła
authors.affiliation: Department of Microbiology, Faculty of Biology, University of Szczecin, Poland; Molecular Biology and Biotechnology Center, Faculty of Biology, University of Szczecin, Poland.
authors.forename: Wiesław
authors.initials: W
authors.lastname: Deptuła
mesh_terms: D000818:Animals; D017250:Caliciviridae Infections / Q000276:immunology / Q000503:physiopathology / Q000662:veterinary*; D016207:Cytokines / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D017929:Hemorrhagic Disease Virus, Rabbit / Q000502:physiology*; D007962:Leukocytes / Q000276:immunology*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D016019:Survival Analysis
chemical_list: D016207:Cytokines; D012333:RNA, Messenger
keywords: Cytokines; RHDV (rabbit haemorrhagic disease virus)