title: [Degradation of Steroidal Hormones by Salt-tolerant Altererythrobacter Strain MH-B5: Degradation Characteristics, Metabolites and Its Immobilization].
abstract: A steroidal hormone degrading bacterium, named MH-B5, was isolated from saline lagoon. Several steroidal hormones including estrone, 17-β-estradiol, estriol and testosterone could be utilized as sole carbon source by strain MH-B5. Analysis of 16S rRNA gene sequence showed that it belonged to genus Altererythrobacter. Growth of strain MH-B5 was observed at salinities of 0-7% and at pH of 5.5-9.0. The main degradation metabolites of testosterone by strain MH-B5 were identified as 4-androstenedione, 1-dehydrotestosterone and androstadienedione using ultra performance liquid chromatography (UPLC) coupled to quadrupole time-of-flight mass spectrometry (Q-TOF MS). Mono-carrier and multi-carrier cell immobilization techniques were successfully applied to this steroid-degrading bacterium. Batch estrogen degradation study showed that immobilized strain MH-B5 could effectively degrade 2 mg · L⁻¹ of 17β-estradiol and its metabolite estrone. The results mentioned above demonstrated the capability of MH-B5 to degrade both estrogens and testosterone and the potential application of using immobilized MH-B5 cells in bioremediation of steroidal hormone pollution in saline environment.
authors.forename: Cong
authors.initials: C
authors.lastname: Ma
authors.forename: Dan
authors.initials: D
authors.lastname: Qin
authors.forename: Qian
authors.initials: Q
authors.lastname: Sun
authors.forename: Chang-ping
authors.initials: CP
authors.lastname: Yu
mesh_terms: D020561:Alphaproteobacteria / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002853:Chromatography, Liquid; D004958:Estradiol; D004967:Estrogens / Q000378:metabolism*; D004970:Estrone; D013058:Mass Spectrometry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D055049:Salt Tolerance; D013256:Steroids / Q000378:metabolism*; D013739:Testosterone / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D004967:Estrogens; D012336:RNA, Ribosomal, 16S; D013256:Steroids; D004970:Estrone; D013739:Testosterone; D004958:Estradiol; C013915:boldenone