title: Triazole-based highly selective supramolecular sensor for the detection of diclofenac in real samples.
abstract: A triazole-based supramolecular chemosensor 6 for the selective spectrophotometric detection of diclofenac in human plasma and tap water was developed. The 6 was synthesized through click approach and characterized via UV-vis spectroscopy, FT-IR, Mass and NMR spectroscopy. The supramolecular interaction of compound 6 with commonly used drugs has been investigated with the help of UV-vis spectral titrations, FT-IR and (1)H, (13)C NMR spectroscopy. The UV-visible spectral changes upon addition of various commonly used drugs showed that compound 6 is highly selective for diclofenac over other drugs. The supramolecule 6 exhibited a selective enhancement in the absorbance intensity after mixing with diclofenac in human plasma and water samples in the presence of other drugs at pH 6-7, with a linear range of 0.938 (R(2)=0.938) and a limit of detection 10µM. The relative standard deviation (RSD) for 100µM diclofenac was found to be 0.462. The Job's plot analysis revealed that diclofenac bind to compound 6 in 1:1 stoichiometry.
authors.affiliation: International Centre for Chemical and Biological Sciences, H.E.J. Research Institute of Chemistry, University of Karachi, Karachi 75270, Pakistan.
authors.forename: Farid
authors.initials: F
authors.lastname: Ahmed
authors.affiliation: International Centre for Chemical and Biological Sciences, H.E.J. Research Institute of Chemistry, University of Karachi, Karachi 75270, Pakistan.
authors.forename: Kiramat
authors.initials: K
authors.lastname: Shah
authors.affiliation: Department of Industrial Chemistry, University of Bologna, Bologna, Italy.
authors.forename: Iqra Zubair
authors.initials: IZ
authors.lastname: Awan
authors.affiliation: International Centre for Chemical and Biological Sciences, H.E.J. Research Institute of Chemistry, University of Karachi, Karachi 75270, Pakistan. Electronic address: raza_shahm@yahoo.com.
authors.forename: Muhammad Raza
authors.initials: MR
authors.lastname: Shah
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D004008:Diclofenac / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D057230:Limit of Detection; D009682:Magnetic Resonance Spectroscopy; D010949:Plasma / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D014230:Triazoles / Q000737:chemistry*; D014867:Water / Q000032:analysis
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D014230:Triazoles; D014867:Water; D004008:Diclofenac
keywords: Chemosensors; Diclofenac; Human blood's plasma; Supramolecular chemistry; Tap water; UV–visible spectrophotometery