title: microRNA-7 Protects Against 1-Methyl-4-Phenylpyridinium Iodide-Induced Cell Apoptosis in SH-SY5Y Cells by Directly Targeting Krüpple-Like Factor 4.
abstract: This study intended to investigate the role and underling mechanism of microRNA-7 (miR-7) on neuronal death in Parkinson's disease (PD). Human neuroblastoma cell line SH-SY5Y was employed and 1-methyl-4-phenylpyridinium iodide [MPP(+)] was used to generate PD model in vitro. Furthermore, an upregulation of miR-7 was performed in SH-SY5Y by transfection with miR-7 mimics. Cell viability and cell apoptosis were determined. Moreover, the target and the mechanism of miR-7 in MPP(+)-induced cell death were also investigated. The upregulation of miR-7 promoted cell viability and suppressed cell apoptosis in MPP(+)-treated SH-SY5Y cells. Furthermore, miR-7 could directly bind to the 3'-untranslated region of Krüppel-like factor 4 (KLF4, positions 574-580). Moreover, knockdown of KLF4 by the specific siRNA inhibited SH-SY5Y apoptosis under MPP(+) treatment. In addition, KLF4 overexpression apparently attenuated the protective effect of miR-7 in MPP(+)-induced SH-SY5Y apoptosis. This study indicated that miR-7 protects from MPP(+)-induced cell apoptosis in SH-SY5Y by directly targeting KLF4.
authors.affiliation: 1 Department of Neurosurgery, The Third People's Hospital of Chengdu , Chengdu, China .
authors.forename: Bin
authors.initials: B
authors.lastname: Kong
authors.affiliation: 2 Department of Neurosurgery, Xianyang Central Hospital , Xianyang, China .
authors.forename: Peng-Chang
authors.initials: PC
authors.lastname: Wu
authors.affiliation: 3 Department of Neurology, Chengdu Military General Hospital , Chengdu, China .
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: 3 Department of Neurology, Chengdu Military General Hospital , Chengdu, China .
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.affiliation: 1 Department of Neurosurgery, The Third People's Hospital of Chengdu , Chengdu, China .
authors.forename: Yu-Qing
authors.initials: YQ
authors.lastname: Yuan
authors.affiliation: 3 Department of Neurology, Chengdu Military General Hospital , Chengdu, China .
authors.forename: Yong-Qin
authors.initials: YQ
authors.lastname: Kuang
authors.affiliation: 3 Department of Neurology, Chengdu Military General Hospital , Chengdu, China .
authors.forename: Lin
authors.initials: L
authors.lastname: Cheng
authors.affiliation: 3 Department of Neurology, Chengdu Military General Hospital , Chengdu, China .
authors.forename: Hu-Tian
authors.initials: HT
authors.lastname: Zhou
authors.affiliation: 4 The 306 Hospital of PLA , Beijing, China .
authors.forename: Jian-Wen
authors.initials: JW
authors.lastname: Gu
mesh_terms: D015655:1-Methyl-4-phenylpyridinium / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism*; D035683:MicroRNAs / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology; D020011:Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D015655:1-Methyl-4-phenylpyridinium; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; D035683:MicroRNAs; D020011:Protective Agents; D017382:Reactive Oxygen Species; C000715467:KLF4 protein, human; C549242:MIRN7-1 microRNA, human