title: Platelet closure time in anesthetized Greyhounds with hemorrhagic shock treated with hydroxyethyl starch 130/0.4 or 0.9% sodium chloride infusions.
abstract: OBJECTIVE
  To measure platelet closure time (PCT) in dogs during controlled hemorrhagic shock and after fluid resuscitation with hydroxyethyl starch (HES) 130/0.4 or 0.9% sodium chloride.
  
  
  METHODS
  Experimental interventional study.
  
  
  METHODS
  University veterinary teaching hospital.
  
  
  METHODS
  Eleven healthy Greyhounds.
  
  
  METHODS
  Dogs were anesthetized and had 48 mL/kg of blood removed to induce hemorrhagic shock. Dogs received 20 mL/kg of HES 130/0.4 (n = 6) or 80 mL/kg of 0.9% sodium chloride (NaCl; n = 5) intravenously over 20 minutes. PCT was measured using the Platelet Function Analyzer-100 with collagen and adenosine-diphosphate cartridges at: T0 = 60 minutes after induction of anesthesia prior to hemorrhage, T1 = during hemorrhagic shock, and T2 = 40 minutes after completion of fluid bolus. Packed cell volume and platelet count were concurrently measured.
  
  
  RESULTS
  Hemorrhagic shock did not significantly change PCT, with no difference between T0 and T1. Both the HES 130/0.4 and 0.9% NaCl group had a significantly increased mean PCT at T2 of 91.4 seconds (95% CI 69.3-113.4) and 95.5 seconds (95% CI 78.2-112.8), respectively, compared to T1. The magnitude of change was significantly greater for the 0.9% NaCl group than the HES 130/0.4 group. There was no difference in the magnitude of change in PCV and platelet count between the 2 groups. The PCV and platelet count were >25% and >100,000/μL, respectively, in all dogs, except for dogs in the HES 130/0.4 group at T2 where platelet counts were <100,000/μL.
  
  
  CONCLUSIONS
  Controlled hemorrhagic shock in Greyhounds under anesthesia did not cause a significant change in PCT. Both HES 130/0.4 and 0.9% NaCl administration after induction of shock increased PCT. These results do not support that HES 130/0.4 causes relevant platelet dysfunction beyond hemodilution.
authors.affiliation: Department of Clinical Sciences and Service, The Royal Veterinary College, University of London, North Mymms, Hertfordshire, UK.
authors.forename: Duana
authors.initials: D
authors.lastname: McBride
authors.affiliation: Department of Clinical Sciences and Service, The Royal Veterinary College, University of London, North Mymms, Hertfordshire, UK.
authors.forename: Giselle
authors.initials: G
authors.lastname: Hosgood
authors.affiliation: Department of Clinical Sciences and Service, The Royal Veterinary College, University of London, North Mymms, Hertfordshire, UK.
authors.forename: Anthea
authors.initials: A
authors.lastname: Raisis
authors.affiliation: School of Veterinary and Life Sciences, College of Veterinary Medicine, Murdoch University, Murdoch Drive, Western Australia, Australia.
authors.forename: Lisa
authors.initials: L
authors.lastname: Smart
mesh_terms: D000758:Anesthesia / Q000662:veterinary*; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects; D004195:Disease Models, Animal; D004285:Dogs / Q000502:physiology*; D005440:Fluid Therapy / Q000379:methods / Q000662:veterinary; D006895:Hydroxyethyl Starch Derivatives / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D007262:Infusions, Intravenous / Q000662:veterinary; D008297:Male; D010976:Platelet Count / Q000662:veterinary; D012771:Shock, Hemorrhagic / Q000628:therapy / Q000662:veterinary*; D012965:Sodium Chloride / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D006895:Hydroxyethyl Starch Derivatives; D012965:Sodium Chloride
keywords: PFA-100; colloids; dog; platelet function