title: Antioxidant and hepatoprotective activity of Hamelia patens extracts.
abstract: Hamelia patens is widely used in the traditional medicine of Mexico and Central America for the treatment of illnesses associated with inflammatory processes. In this study, antioxidant and hepatoprotective activity were assayed on the methanolic crude (ME), hexane (HE), ethyl acetate (AE), and butanol (BE) extracts of H. patens. The total phenolic content (TPC) as mg of gallic acid equivalents per g of dry extract was determined by Folin-Ciocalteu's method (ME=141.58±11.99, HE=33.96±1.13, AE=375.18±13.09, BE=132.08±3.62), and antioxidant activity by 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free radical-scavenging method (EC(50) ME=77.87±5.67, HE=236.64±26.32, AE=45.87±2.24, BE=50.97±0.85μg/mL). Hepatoprotective activity was evaluated through AST activity on HepG2 cells subjected to damage with CCl(4) (ME=62.5±3.41, HE=72.25±2.87, AE=63.50±4.20, BE=43.74±4.03). BE showed the greater hepatoprotective activity and a good antioxidant capacity, while HE did not show hepatoprotective or antioxidant activity. Cytotoxicity was evaluated on Vero cells cultures; none showed significant toxicity.
authors.affiliation: Universidad Autónoma de Nuevo León, Departamento de Química Analítica, Facultad de Medicina. Av. Fancisco I. Madero y Dr Aguirre Pequeño S/N. Col Mitras Centro. CP 64460. Monterrey, NL. México.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Perez-Meseguer
authors.affiliation: Universidad Autónoma de Nuevo León, Departamento de Química Analítica, Facultad de Medicina. Av. Fancisco I. Madero y Dr Aguirre Pequeño S/N. Col Mitras Centro. CP 64460. Monterrey, NL. México.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Delgado-Montemayor
authors.affiliation: Universidad Autónoma de Nuevo León, Departamento de Química Analítica, Facultad de Medicina. Av. Fancisco I. Madero y Dr Aguirre Pequeño S/N. Col Mitras Centro. CP 64460. Monterrey, NL. México.
authors.forename: Tania
authors.initials: T
authors.lastname: Ortíz-Torres
authors.affiliation: Universidad Autónoma de Nuevo León, Departamento de Química Analítica, Facultad de Medicina. Av. Fancisco I. Madero y Dr Aguirre Pequeño S/N. Col Mitras Centro. CP 64460. Monterrey, NL. México.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Salazar-Aranda
authors.affiliation: Universidad Autónoma de Nuevo León, Unidad de Hígado. Facultad de Medicina y Hospital Universitario "Dr. José Eleuterio González. Av. Fancisco I. Madero y Dr Aguirre Pequeño S/N. Col Mitras Centro, CP 64460. Monterrey, NL. México.
authors.forename: Paula
authors.initials: P
authors.lastname: Cordero-Perez
authors.affiliation: Universidad Autónoma de Nuevo León, Departamento de Química Analítica, Facultad de Medicina. Av. Fancisco I. Madero y Dr Aguirre Pequeño S/N. Col Mitras Centro. CP 64460. Monterrey, NL. México.
authors.forename: Noemí Waksman
authors.initials: NW
authors.lastname: de Torres
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001219:Aspartate Aminotransferases / Q000032:analysis; D001713:Biphenyl Compounds; D002252:Carbon Tetrachloride Poisoning / Q000473:pathology / Q000517:prevention & control; D002460:Cell Line; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control*; D002522:Chlorocebus aethiops; D016166:Free Radical Scavengers / Q000494:pharmacology; D032063:Hamelia / Q000737:chemistry*; D006801:Humans; D010636:Phenols / Q000032:analysis; D010851:Picrates; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D059808:Polyphenols / Q000737:chemistry / Q000494:pharmacology; D014709:Vero Cells
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; C004931:1,1-diphenyl-2-picrylhydrazyl; D001219:Aspartate Aminotransferases