title: Freeze-dried egg yolk based extenders containing various antioxidants improve post-thawing quality and incubation resilience of goat spermatozoa.
abstract: The aim of this study was to evaluate different antioxidants-supplemented freeze-dried egg yolk based extenders for the post-thawing quality and incubation resilience of goat spermatozoa. Pooled semen were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in control and antoxidant supplemented freeze-dried extenders (methionine, cysteamine and butylated hydroxytoluene). Semen samples were assessed for sperm motility, plasma membrane functional integrity using hypoosmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). Membrane lipid peroxidation status was also analyzed using the malondialdehyde (MDA) concentration. In the study, antioxidant supplemented freeze-dried egg yolk based extenders have beneficial effect on goat sperm parameters. In addition, we achieved a higher quality in post thawed goat semen even after 6 h incubation when the extender was supplemented by 5 mM BHT or cysteamine.
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle/Bursa 16059, Turkey. Electronic address: salcay@uludag.edu.tr.
authors.forename: Selim
authors.initials: S
authors.lastname: Alcay
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle/Bursa 16059, Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Gokce
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle/Bursa 16059, Turkey.
authors.forename: M Berk
authors.initials: MB
authors.lastname: Toker
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle/Bursa 16059, Turkey.
authors.forename: N Tekin
authors.initials: NT
authors.lastname: Onder
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle/Bursa 16059, Turkey.
authors.forename: Burcu
authors.initials: B
authors.lastname: Ustuner
authors.affiliation: Department of Biostatistics, Faculty of Veterinary Medicine, Uludag University, Gorukle/Bursa 16059, Turkey.
authors.forename: Ender
authors.initials: E
authors.lastname: Uzabacı
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Uludag University, Gorukle/Bursa 16059, Turkey.
authors.forename: Zulfiye
authors.initials: Z
authors.lastname: Gul
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle/Bursa 16059, Turkey.
authors.forename: Seda
authors.initials: S
authors.lastname: Cavus
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods*; D003543:Cysteamine / Q000494:pharmacology; D004530:Egg Yolk; D006041:Goats; D020287:In Situ Nick-End Labeling; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D012661:Semen; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility; D013094:Spermatozoa
chemical_list: D000975:Antioxidants; D002084:Butylated Hydroxytoluene; D008315:Malondialdehyde; D003543:Cysteamine
keywords: Antioxidants; Cryopreservation; Freeze-drying; Goat semen; Incubation resilience