title: Analysis of Gene Expression in the Endocervical Epithelium of Women With Deep Endometriosis.
abstract: Endometriosis affects approximately 12% of reproductive-age women and is currently diagnosed using invasive laparoscopic surgery. Differences in gene expression in the eutopic endometrium between women with and without endometriosis have been reported, and determining the reproducibility of these genetic differences in the endocervical epithelium would represent an important step toward developing novel diagnostic strategies. In this study, we analyzed gene expression in the endocervical epithelium in women with and without moderate or severe endometriosis. Using RT2 Profiler PCR Arrays, we analyzed gene expression in endocervical epithelial cells from women with deep endometriosis (n = 4) and healthy women (n =6). Nine genes were identified as being upregulated: 5 cell cycle genes (cyclin B1 [CCNB1], cyclin G1 [CCNG1], cullin 1 [CUL1], general transcription factor IIH, polypeptide 1 [GTF2H1], and proliferating cell nuclear antigen [PCNA]), 3 cytokine genes (C3, chemokine (C-C motif) ligand 21 [CCL21], and chemokine (C-X-C motif) ligand 14 [CXCL14]) and 1 gene related to dendritic cell pathways (ICAM2), showing that differential gene expression is present in the endocervical epithelium of women with deep endometriosis.
authors.affiliation: Pelvic Pain and Endometriosis Unit, Gynecology Department, Paulista School of Medicine, Federal University of Sao Paulo (EPM-UNIFESP), São Paulo, Brazil akopelman@gmail.com.
authors.forename: Alexander
authors.initials: A
authors.lastname: Kopelman
authors.affiliation: Pelvic Pain and Endometriosis Unit, Gynecology Department, Paulista School of Medicine, Federal University of Sao Paulo (EPM-UNIFESP), São Paulo, Brazil.
authors.forename: Manoel J B C
authors.initials: MJ
authors.lastname: Girão
authors.affiliation: Pelvic Pain and Endometriosis Unit, Gynecology Department, Paulista School of Medicine, Federal University of Sao Paulo (EPM-UNIFESP), São Paulo, Brazil.
authors.forename: Tatiana C S
authors.initials: TC
authors.lastname: Bonetti
authors.affiliation: Pelvic Pain and Endometriosis Unit, Gynecology Department, Paulista School of Medicine, Federal University of Sao Paulo (EPM-UNIFESP), São Paulo, Brazil.
authors.forename: Cristina V
authors.initials: CV
authors.lastname: Carvalho
authors.affiliation: Molecular and Metabolomics Laboratory, Department of Gynecology, Universidade Federal de São Paulo - Escola Paulista de Medicina (EPM-UNIFESP), São Paulo, Brazil.
authors.forename: Ismael Dale Cotrim Guerreiro
authors.initials: ID
authors.lastname: da Silva
authors.affiliation: Pelvic Pain and Endometriosis Unit, Gynecology Department, Paulista School of Medicine, Federal University of Sao Paulo (EPM-UNIFESP), São Paulo, Brazil.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Schor
mesh_terms: D000328:Adult; D004715:Endometriosis / Q000235:genetics* / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D014599:Uterus / Q000378:metabolism*
keywords: endocervix; endometriosis; gene expression; noninvasive diagnosis