title: Evaluation of oral Lanzhou lamb rotavirus vaccine via passive transfusion with CD4(+)/CD8(+) T lymphocytes.
abstract: Lanzhou Lamb derived Rotavirus (RV) Vaccine (namely LLR) for children is only used in China. Since there were no reports on evaluation of LLR, even the data of phase IV clinical trial, we proceed the evaluation of LLR through focusing on T-cell to investigate whether LLR could induce the potential function involving in protection as a vaccine. Four groups of nude mice were transfused with CD4(+)/CD8(+) T-cells isolated from LLR-immunized (primed) and LLR-unimmunized (naïve) mice via intraperitonea (i.p.) respectively. Consequently, the adoption mice were challenged with mice-origin wild rotavirus EDIM (Epizootic Diarrhea of Infant Mice) by intragastric administration. Series of fecal/serum samples were collected and viral shedding, then serum IgA/IgG and secreted IgA were assayed. Compared to the mice transfused with T lymphocytes from naïve mice, the nude mice transfused with CD4(+) T lymphocytes from primed mice induce fecal and serum IgA increasing more rapidly, and have a shorter duration of virus shedding too. Whereas, no significant difference in virus clearance was found between the mice transfused with CD8(+) T lymphocytes isolated from primed and naïve mice. Therefore, we cleared the distinct roles of transfused CD4(+)/CD8(+) T lymphocytes for rotavirus clearance in nude mice, that the viral clearance conducted by CD4(+) T lymphocytes. Meanwhile, it has ability to help induction of LLR specific immunogenicity. Comparing with the transfusion of cell from primed and naïve mice, LLR can induce CD4(+) T lymphocytes memory which is a potential index to reflect the immunogenicity and protection, while CD8(+) T lymphocytes remove rotavirus by CTL with little memory ability.
authors.affiliation: Division of Enteric Viral Vaccines, National Institutes for Food and Drug Control, Beijing, 100050, China.
authors.forename: Jialiang
authors.initials: J
authors.lastname: Du
authors.affiliation: Division of Enteric Viral Vaccines, National Institutes for Food and Drug Control, Beijing, 100050, China; National Vaccine & Serum Institute, Beijing, China.
authors.forename: Zhiling
authors.initials: Z
authors.lastname: Lan
authors.affiliation: Division of Enteric Viral Vaccines, National Institutes for Food and Drug Control, Beijing, 100050, China.
authors.forename: Yueyue
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Division of Enteric Viral Vaccines, National Institutes for Food and Drug Control, Beijing, 100050, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Division of Enteric Viral Vaccines, National Institutes for Food and Drug Control, Beijing, 100050, China.
authors.forename: Qingchuan
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Division of Enteric Viral Vaccines, National Institutes for Food and Drug Control, Beijing, 100050, China; University of Pennsylvania, USA.
authors.forename: Yanchao
authors.initials: Y
authors.lastname: Li
authors.affiliation: Division of Enteric Viral Vaccines, National Institutes for Food and Drug Control, Beijing, 100050, China. Electronic address: guotai@nifdc.org.cn.
authors.forename: Tai
authors.initials: T
authors.lastname: Guo
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D002469:Cell Separation; D005260:Female; D007070:Immunoglobulin A / Q000097:blood; D007071:Immunoglobulin A, Secretory / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D012400:Rotavirus Infections; D022243:Rotavirus Vaccines / Q000276:immunology*; D017201:Virus Shedding
chemical_list: D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D022243:Rotavirus Vaccines
keywords: CD4(+)/CD8(+) T lymphocytes; LLR; Nude mouse; Rotavirus vaccine; Transfusion