title: Autologous peripheral blood stem cell harvest: Collection efficiency and factors affecting it.
abstract: BACKGROUND
  Harvest of hematopoietic progenitor cells via leukapheresis is being used increasingly for transplants in India. Adequate yield of cells per kilogram body weight of recipient is required for successful engraftment. Collection efficiency (CE) is an objective quality parameter used to assess the quality of leukapheresis program. In this study, we calculated the CE of the ComTec cell separator (Fresenius Kabi, Germany) using two different formulae (CE1 and CE2) and analyzed various patient and procedural factors, which may affect it.
  
  
  METHODS
  One hundred and one consecutive procedures in 77 autologous donors carried out over 3 years period were retrospectively reviewed. Various characteristics like gender, age, weight, disease status, hematocrit, preprocedure total leukocyte count, preprocedure CD34 positive (CD34+) cells count, preprocedure absolute CD34+ cell count and processed apheresis volume effect on CE were compared. CE for each procedure was calculated using two different formulae, and results were compared using statistical correlation and regression analysis.
  
  
  RESULTS
  The mean CE1 and CE2 was 41.2 and 49.1, respectively. CE2 appeared to be more accurate indicator of overall CE as it considered the impact of continued mobilization of stem cells during apheresis procedure, itself. Of all the factors affecting CE, preprocedure absolute CD34+ was the only independent factor affecting CE.
  
  
  CONCLUSIONS
  The only factor affecting CE was preprocedure absolute CD34+ cells. Though the mean CE2 was higher than CE1, it was not statistically significant.
authors.affiliation: Department of Transfusion Medicine, Medanta-The Medicity, Sector-38, Gurgaon, Haryana, India.
authors.forename: Aseem K
authors.initials: AK
authors.lastname: Tiwari
authors.affiliation: Department of Transfusion Medicine, Jaypee Hospital, Sector-128, Noida, Uttar Pradesh, India.
authors.forename: Prashant
authors.initials: P
authors.lastname: Pandey
authors.affiliation: Department of Transfusion Medicine, Medanta-The Medicity, Sector-38, Gurgaon, Haryana, India.
authors.forename: Harini
authors.initials: H
authors.lastname: Subbaraman
authors.affiliation: Department of Hemato-Oncology, Artemis Hospital, Sector-51, Gurgaon, Haryana, India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Bhargava
authors.affiliation: Department of Transfusion Medicine, Medanta-The Medicity, Sector-38, Gurgaon, Haryana, India.
authors.forename: Ganesh
authors.initials: G
authors.lastname: Rawat
authors.affiliation: Department of Transfusion Medicine, Medanta-The Medicity, Sector-38, Gurgaon, Haryana, India.
authors.forename: Shivani
authors.initials: S
authors.lastname: Madiraju
authors.affiliation: Department of Transfusion Medicine, Medanta-The Medicity, Sector-38, Gurgaon, Haryana, India.
authors.forename: Vimarsh
authors.initials: V
authors.lastname: Raina
authors.affiliation: Department of Gastroenterology, Artemis Hospital, Sector-51, Gurgaon, Haryana, India.
authors.forename: Richa
authors.initials: R
authors.lastname: Bhargava
keywords: Collection efficiency; leukapheresis; peripheral blood stem cells