title: Detection of Antileukocytic Antibodies in Blood Serum using Lymphocytes and Latex Microspheres Carrying HLA-Antigens upon Alloimmunization of Women with Recurrent Pregnancy Loss.
abstract: Anti-HLA-antibodies were detected using cross-reaction of blood serum with allogenic T and B cells and latex microspheres coated with HLA-I and HLA-II antigens. HLA+ and HLA-sera obtained from women before and after allogeneic immunization were tested. The results obtained by these methods significantly differed. The test with latex microspheres detected antibodies to HLA-I and HLA-II antigens with high sensitivity and specificity and can be used for assessment of clinical significance of alloantibody detection when using alloimmunization in the therapy of gestation disorders.
authors.affiliation: V. I. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia. elena2404.07@mail.ru.
authors.forename: E O
authors.initials: EO
authors.lastname: Stepanova
authors.affiliation: V. I. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: M A
authors.initials: MA
authors.lastname: Nikolaeva
authors.affiliation: V. I. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: E L
authors.initials: EL
authors.lastname: Golubeva
authors.affiliation: V. I. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Vtorushina
authors.affiliation: V. I. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: L V
authors.initials: LV
authors.lastname: Van'ko
authors.affiliation: V. I. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: Z S
authors.initials: ZS
authors.lastname: Khodzhaeva
authors.affiliation: V. I. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: L V
authors.initials: LV
authors.lastname: Krechetova
mesh_terms: D000026:Abortion, Habitual / Q000276:immunology*; D000906:Antibodies / Q000097:blood* / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D007962:Leukocytes / Q000276:immunology*; D008863:Microspheres; D011247:Pregnancy; D012680:Sensitivity and Specificity; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000906:Antibodies; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II
keywords: HLA; alloimmunization; antibodies to human leukocyte antigens; flow cytometry; recurrent abortion