title: Selective Cell Elimination from Mixed 3D Culture Using a Near Infrared Photoimmunotherapy Technique.
abstract: Recent developments in tissue engineering offer innovative solutions for many diseases. For example, tissue engineering using induced pluripotent stem cell (iPS) emerged as a new method in regenerative medicine. Although this tissue regeneration is promising, contamination with unwanted cells during tissue cultures is a major concern. Moreover, there is a safety concern regarding tumorigenicity after transplantation. Therefore, there is an urgent need for eliminating specific cells without damaging other cells that need to be protected, especially in established tissue. Here, we present a method for specific cell elimination from a mixed 3D cell culture in vitro with near infrared photoimmunotherapy (NIR-PIT) without damaging non-targeted cells. This technique enables the elimination of specific cells from mixed cell cultures or tissues.
authors.affiliation: Molecular Imaging Program, National Cancer Institute.
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Sato
authors.affiliation: Molecular Imaging Program, National Cancer Institute.
authors.forename: Peter L
authors.initials: PL
authors.lastname: Choyke
authors.affiliation: Molecular Imaging Program, National Cancer Institute; kobayash@mail.nih.gov.
authors.forename: Kobayashi
authors.initials: K
authors.lastname: Hisataka
mesh_terms: D016475:3T3 Cells; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D006801:Humans; D007167:Immunotherapy / Q000379:methods*; D057026:Induced Pluripotent Stem Cells / Q000502:physiology*; D051379:Mice; D010789:Phototherapy / Q000379:methods*; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods