title: Cloning and characterization of filamentous temperature-sensitive protein Z from Xanthomonas oryzae pv. Oryzae.
abstract: The ftsZ gene from Xanthomonas oryzae pv. Oryzae was amplified by PCR with the specific primers, and the recombinant plasmid pET-22b-ftsZ was constructed successfully. The FtsZ with a 6× His tag was overexpressed in a soluble form in Escherichia coli BL21 and purified through a Ni-NTA agarose column. The purified recombinant FtsZ showed a single band on SDS-PAGE with an apparent molecular mass of about 44 kDa, and confirmed by western blotting analysis. The optimum temperature for GTPase activity of the recombined FtsZ was 50 °C, and the optimum pH was 7.0. The recombinant FtsZ showed good stability and retained >95 % activity at 50 °C for 240 min. The GTPase activity followed Michaelis-Menten kinetics with the KM of 1.750 mM and the Vmax of 0.155 nmol Pi/min/nmol FtsZ respectively.
authors.affiliation: College of Life Science, Jiangxi Provincial Key Lab of Protection and Utilization of Subtropical Plant Resources, Jiangxi Normal University, Nanchang, 330022 Jiangxi China.
authors.forename: Leng
authors.initials: L
authors.lastname: Dai
authors.affiliation: College of Life Science, Jiangxi Provincial Key Lab of Protection and Utilization of Subtropical Plant Resources, Jiangxi Normal University, Nanchang, 330022 Jiangxi China.
authors.forename: Yunhong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: College of Life Science, Jiangxi Provincial Key Lab of Protection and Utilization of Subtropical Plant Resources, Jiangxi Normal University, Nanchang, 330022 Jiangxi China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Life Science, Jiangxi Provincial Key Lab of Protection and Utilization of Subtropical Plant Resources, Jiangxi Normal University, Nanchang, 330022 Jiangxi China.
authors.forename: Zhong-Er
authors.initials: ZE
authors.lastname: Long
keywords: Escherichia coli BL21; Expression; FtsZ; Xanthomonas oryzae pv. Oryzae