title: Increased 8-hydroxy-2'-deoxyguanosine in leukocyte DNA from patients with type 2 diabetes and microangiopathy.
abstract: OBJECTIVE
  To evaluate oxidative damage in leukocytes from patients with type 2 diabetes by examining 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels.
  
  
  METHODS
  Patients with type 2 diabetes and healthy controls were assessed for demographic, clinical and biochemical characteristics. Levels of 8-OHdG in extracted leukocyte DNA were determined by enzyme linked immunosorbent assay.
  
  
  RESULTS
  Of 108 patients with type 2 diabetes (56 with microangiopathy, 52 without) and 65 healthy controls, leukocyte 8-OHdG levels were higher in patients with type 2 diabetes versus controls (median ± interquartile range [IQR], 3.19 ± 2.17 versus 0.38 ± 1.00 ng/ml), and higher in patients with type 2 diabetes and microangiopathy versus those without microangiopathy (median ± IQR, 3.34 ± 1.87 versus 2.71 ± 2.26 ng/ml). Patients with type 2 diabetes and microangiopathy had higher serum creatinine and urinary albumin levels versus those without microangiopathy. Leukocyte 8-OHdG levels, duration of type 2 diabetes, albuminuria, use of insulin and use of angiotensin-converting enzyme (ACE) inhibitors/angiotensin receptor blockers (ARBs) were independently associated with microangiopathy in patients with type 2 diabetes after adjustment for smoking.
  
  
  CONCLUSIONS
  Leukocyte oxidative DNA damage was high in patients with type 2 diabetes and microangiopathy. Presence of microangiopathy was associated with leukocyte 8-OHdG levels, duration of type 2 diabetes, albuminuria and use of ACE inhibitors/ARBs or insulin.
authors.affiliation: Department of Endocrinology, Jinling Hospital, Southern Medical School, Nanjing, Jiangsu Province, China.
authors.forename: Xiaozhen
authors.initials: X
authors.lastname: Ye
authors.affiliation: Department of Endocrinology, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu Province, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Jiang
authors.affiliation: Department of Endocrinology, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu Province, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu Province, China.
authors.forename: Ruifeng
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Endocrinology, Jinling Hospital, Nanjing, Jiangsu Province, China.
authors.forename: Cuihua
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Endocrinology, Jinling Hospital, Nanjing, Jiangsu Province, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Endocrinology, Jinling Hospital, Nanjing, Jiangsu Province, China duhong0810@sina.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Du
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D044466:Asian People; D016022:Case-Control Studies; D004247:DNA / Q000378:metabolism*; D004249:DNA Damage; D003710:Demography; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000378:metabolism*; D003925:Diabetic Angiopathies / Q000150:complications / Q000378:metabolism*; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis
chemical_list: D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine
keywords: 8-hydroxy-2’-deoxyguanosine; DNA damage; Type 2 diabetes; diabetic microangiopathy; oxidative stress