title: The clinical relevance and prognostic significance of microsomal epoxide hydrolase gene polymorphisms and their susceptibility to acquired aplastic anemia: an Egyptian study.
abstract: BACKGROUND
  Microsomal epoxide hydrolase enzyme (mEPHX) is involved in xenobiotics detoxification. Two variants of mEPHX, Tyr113His and His139Arg, have been described. Both may lead to acquired aplastic anemia (AA).
  
  
  OBJECTIVE
  Assessing mEPHX genetic polymorphisms and detecting their impact on susceptibility and prognosis in Egyptian AA patients.
  
  
  METHODS
  mEPHX 113 and 139 genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 100 patients with AA and 100 control subjects.
  
  
  RESULTS
  Both mEPHX Tyr113His and His139Arg gene polymorphisms were associated with increased risk of developing AA, and have a significant impact of bad prognosis (p value < 0.01).
  
  
  CONCLUSIONS
  These mEPHX gene polymorphisms can be considered as risk factors and predictive molecular markers for prognosis in AA patients.
authors.affiliation: a Department of Clinical and Chemical Pathology , Faculty of Medicine, Cairo University , Cairo , Egypt and.
authors.forename: Manal Mohamed
authors.initials: MM
authors.lastname: Makhlouf
authors.affiliation: b Department of Pediatric , Faculty of Medicine, Cairo University , Cairo , Egypt.
authors.forename: Rania Ismail
authors.initials: RI
authors.lastname: Magdy
mesh_terms: D000741:Anemia, Aplastic / Q000175:diagnosis* / Q000235:genetics; D015415:Biomarkers; D016022:Case-Control Studies; D004534:Egypt; D004851:Epoxide Hydrolases / Q000235:genetics*; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011379:Prognosis; D012307:Risk Factors
chemical_list: D015415:Biomarkers; D005819:Genetic Markers; D004851:Epoxide Hydrolases
keywords: Acquired aplastic anemia; PCR-RFLP; genetic polymorphisms; mEPHX His139Arg; mEPHX Tyr113His