title: Quantitative super-resolution imaging with qPAINT.
abstract: Counting molecules in complexes is challenging, even with super-resolution microscopy. Here, we use the programmable and specific binding of dye-labeled DNA probes to count integer numbers of targets. This method, called quantitative points accumulation in nanoscale topography (qPAINT), works independently of dye photophysics for robust counting with high precision and accuracy over a wide dynamic range. qPAINT was benchmarked on DNA nanostructures and demonstrated for cellular applications by quantifying proteins in situ and the number of single-molecule FISH probes bound to an mRNA target.
authors.affiliation: Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA.|Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Ralf
authors.initials: R
authors.lastname: Jungmann
authors.affiliation: Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA.|Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Maier S
authors.initials: MS
authors.lastname: Avendaño
authors.affiliation: Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA.|Harvard Program in Biophysics, Harvard University, Boston, Massachusetts, USA.
authors.forename: Mingjie
authors.identifier: 0000-0002-8665-4966
authors.initials: M
authors.lastname: Dai
authors.affiliation: Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA.
authors.forename: Johannes B
authors.initials: JB
authors.lastname: Woehrstein
authors.affiliation: Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA.
authors.forename: Sarit S
authors.initials: SS
authors.lastname: Agasti
authors.affiliation: Rosenstiel Basic Medical Sciences Research Center, Department of Biology, Brandeis University, Waltham, Massachusetts, USA.
authors.forename: Zachary
authors.initials: Z
authors.lastname: Feiger
authors.affiliation: Rosenstiel Basic Medical Sciences Research Center, Department of Biology, Brandeis University, Waltham, Massachusetts, USA.
authors.forename: Avital
authors.identifier: 0000-0002-2051-8304
authors.initials: A
authors.lastname: Rodal
authors.affiliation: Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA.|Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Peng
authors.initials: P
authors.lastname: Yin
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods*; D062105:Molecular Docking Simulation; D017422:Sequence Analysis, DNA; D012984:Software
chemical_list: D005456:Fluorescent Dyes; D004247:DNA