title: Proteomic and metabolomic analysis on the toxicological effects of Benzo[a]pyrene in pearl oyster Pinctada martensii.
abstract: Benzo[a]pyrene (BaP) is one of the typical toxic polycyclic aromatic hydrocarbons (PAHs) that are widely present in marine environment. BaP has diverse toxic effects, including teratogenic, carcinogenic, mutagenic effects and so on, in various organisms. In this work, we focused on the differential proteomic and metabolomic responses in the digestive gland of pearl oyster Pinctada martensii exposed to two doses of BaP (1 and 10μg/L). Metabolic responses revealed that the high dose of BaP (10μg/L) mainly caused disturbances in osmotic regulation and energy metabolism in the digestive gland. Proteomic responses indicated that both doses of BaP induced disturbances in energy metabolism, cytoskeleton, cell injury, oxidative stress and signal transduction based on the differential proteomic biomarkers. Overall, these results demonstrated a number of potential biomarkers that were characterized by an integrated proteomic and metabolomic approach and provided a useful insight into the toxicological effects on pearl oyster P. martensii.
authors.affiliation: College of Environment and Plant Protection, Hainan University, Haikou 570228, China; Haikou Key Laboratory of Environmental Toxicology, Haikou 570228, China. Electronic address: chenhao.4788@hotmail.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.affiliation: College of Agriculture, Hainan University, Haikou 570228, China. Electronic address: 2692988677@qq.com.
authors.forename: Qinqin
authors.initials: Q
authors.lastname: Song
authors.affiliation: College of Agriculture, Hainan University, Haikou 570228, China; Haikou Key Laboratory of Environmental Toxicology, Haikou 570228, China. Electronic address: diaoxip@hainu.edu.cn.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Diao
authors.affiliation: College of Agriculture, Hainan University, Haikou 570228, China; Haikou Key Laboratory of Environmental Toxicology, Haikou 570228, China. Electronic address: zhouhl@hainu.edu.cn.
authors.forename: Hailong
authors.initials: H
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D015415:Biomarkers / Q000378:metabolism; D003599:Cytoskeleton / Q000187:drug effects; D004064:Digestive System / Q000187:drug effects*; D004734:Energy Metabolism / Q000187:drug effects; D055432:Metabolomics; D018384:Oxidative Stress / Q000187:drug effects; D049911:Pinctada / Q000187:drug effects*; D040901:Proteomics / Q000379:methods; D015398:Signal Transduction / Q000187:drug effects; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D014874:Water Pollutants, Chemical; D001564:Benzo(a)pyrene
keywords: Benzo[a]pyrene; Biomarker; Metabolomics; Pinctada martensii; Proteomics