title: Comparing Raman and fluorescence lifetime spectroscopy from human atherosclerotic lesions using a bimodal probe.
abstract: Fluorescence lifetime imaging (FLIm) and Raman spectroscopy are two promising methods to support morphological intravascular imaging techniques with chemical contrast. Both approaches are complementary and may also be used in combination with OCT/IVUS to add chemical specificity to these morphologic intravascular imaging modalities. In this contribution, both modalities were simultaneously acquired from two human coronary specimens using a bimodal probe. A previously trained SVM model was used to interpret the fluorescence lifetime data; integrated band intensities displayed in RGB false color images were used to interpret the Raman data. Both modalities demonstrate unique strengths and weaknesses and these will be discussed in comparison to histologic analyses from the two coronary arteries imaged.
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Dochow
authors.affiliation: Department of Biomedical Engineering, University of California, Davis, 451 E. Health Sciences Drive, Davis, CA 95616, USA.
authors.forename: Hussain
authors.initials: H
authors.lastname: Fatakdawala
authors.affiliation: Department of Biomedical Engineering, University of California, Davis, 451 E. Health Sciences Drive, Davis, CA 95616, USA.
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Phipps
authors.affiliation: Department of Biomedical Engineering, University of California, Davis, 451 E. Health Sciences Drive, Davis, CA 95616, USA.
authors.forename: Dinglong
authors.initials: D
authors.lastname: Ma
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Bocklitz
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Schmitt
authors.affiliation: Department of Pathology & Laboratory Medicine, University of California Davis Medical Center, Davis, CA, USA.
authors.forename: John W
authors.initials: JW
authors.lastname: Bishop
authors.affiliation: Cardiovascular Institute, Perelman School of Medicine, , University of Pennsylvania, Philadelphia, PA, USA.
authors.forename: Kenneth B
authors.initials: KB
authors.lastname: Margulies
authors.affiliation: Department of Biomedical Engineering, University of California, Davis, 451 E. Health Sciences Drive, Davis, CA 95616, USA. lmarcu@ucdavis.edu.
authors.forename: Laura
authors.initials: L
authors.lastname: Marcu
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany. juergen.popp@ipht-jena.de.|Leibniz Institute of Photonic Technology Jena e.V., Albert-Einstein-Str. 9, 07745, Jena, Germany. juergen.popp@ipht-jena.de.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Popp
mesh_terms: D050197:Atherosclerosis / Q000000981:diagnostic imaging*; D003331:Coronary Vessels / Q000000981:diagnostic imaging*; D006801:Humans; D009206:Myocardium / Q000473:pathology; D061848:Optical Imaging; D013050:Spectrometry, Fluorescence; D013059:Spectrum Analysis, Raman
keywords: Raman spectroscopy; atherosclerosis; bimodal; fiber probes; fluorescence lifetime; imaging