title: Polymorphic microsatellite loci for Haumania danckelmaniana and transferability to H. liebrechtsiana (Marantaceae).
abstract: OBJECTIVE
  Microsatellite markers were developed for the species Haumania danckelmaniana (Marantaceae) from central tropical Africa.
  
  
  RESULTS
  Microsatellite isolation was performed simultaneously on three different species of Marantaceae through a procedure that combines multiplex microsatellite enrichment and next-generation sequencing. From 80 primers selected for initial screening, 20 markers positively amplified in H. danckelmaniana, of which 10 presented unambiguous amplification products within the expected size range and eight were polymorphic with four to nine alleles per locus. Positive transferability with the related species H. liebrechtsiana was observed for the same 10 markers.
  
  
  CONCLUSIONS
  The polymorphic microsatellite markers are suitable for studies in genetic diversity and structure, mating system, and gene flow in H. danckelmaniana and the closely related species H. liebrechtsiana.
authors.affiliation: Institut für Geobotanik und Botanischer Garten, Martin-Luther-Universität Halle-Wittenberg, Im Neuwerk 21, 06108 Halle (Saale), Germany; Evolutionary Biology and Ecology, CP160/12, Faculté des Sciences, Université Libre de Bruxelles, 50 Av. F. Roosevelt, 1050 Brussels, Belgium.
authors.forename: Alexandra C
authors.initials: AC
authors.lastname: Ley
authors.affiliation: Evolutionary Biology and Ecology, CP160/12, Faculté des Sciences, Université Libre de Bruxelles, 50 Av. F. Roosevelt, 1050 Brussels, Belgium.
authors.forename: Olivier J
authors.initials: OJ
authors.lastname: Hardy
keywords: Africa; Haumania; Marantaceae; phylogeography; rainforest; simple sequence repeat (SSR)