title: Structure of the O-specific polysaccharide from a marine bacterium Echinicola pacifica КММ 6172(Т) containing 2,3-diacetamido-2,3-dideoxy-D-glucuronic acid.
abstract: The O-polysaccharide was isolated from the lipopolysaccharide of Echinicola pacifica KMM 6172(T) and studied by chemical analyses along with (1)H and (13)C NMR spectroscopy, including (1)H, (1)H COSY, 1D and 2D TOCSY, ROESY, (1)H, (13)С HMQC, HMBC and H2BC experiments. It was found that the polysaccharide is built up of branched pentasaccharide repeating units, containing D-galactose (Gal), L-rhamnose (Rha), 2-acetamido-2-deoxy-D-glucose (GlcNAc), two residues of 2,3-diacetamido-2,3-dideoxy-D-glucuronic acid (GlcNAc3NAcA) and O-acetyl group in nonstoichiometric amount and has the following structure [Structure: see text].
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia. Electronic address: tomsh@piboc.dvo.ru.
authors.forename: Svetlana V
authors.initials: SV
authors.lastname: Tomshich
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia.
authors.forename: Maxim S
authors.initials: MS
authors.lastname: Kokoulin
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia.
authors.forename: Anatoliy I
authors.initials: AI
authors.lastname: Kalinovsky
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia.
authors.forename: Ol'ga I
authors.initials: OI
authors.lastname: Nedashkovskaya
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia.
authors.forename: Nadezhda A
authors.initials: NA
authors.lastname: Komandrova
mesh_terms: D002236:Carbohydrate Conformation; D041964:Cytophagaceae / Q000737:chemistry*; D005965:Glucuronates / Q000032:analysis / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D005965:Glucuronates; D011134:Polysaccharides; C031522:2,3-diacetamido-2,3-dideoxy-glucuronic acid
keywords: 2,3-diacetamido-2,3-dideoxy-D-glucuronic acid (GlcNAc3NAcA); Bacterial polysaccharide structure; Echinicola pacifica; Marine bacterium; O-specific polysaccharide