title: Copper-Containing Anti-Biofilm Nanofiber Scaffolds as a Wound Dressing Material.
abstract: Copper particles were incorporated into nanofibers during the electrospinning of poly-D,L-lactide (PDLLA) and poly(ethylene oxide) (PEO). The ability of the nanofibers to prevent Pseudomonas aeruginosa PA01 and Staphylococcus aureus (strain Xen 30) to form biofilms was tested. Nanofibers containing copper particles (Cu-F) were thinner (326 ± 149 nm in diameter), compared to nanofibers without copper (CF; 445 ± 93 nm in diameter). The crystalline structure of the copper particles in Cu-F was confirmed by X-ray diffraction (XRD). Copper crystals were encapsulated, but also attached to the surface of Cu-F, as shown scanning transmission electron microscopy (STEM) and transmission electron microscopy (TEM), respectively. The copper particles had no effect on the thermal degradation and thermal behaviour of Cu-F, as shown by thermogravimetric analysis (TGA) and differential scanning calorimeter (DSC). After 48 h in the presence of Cu-F, biofilm formation by P. aeruginosa PA01 and S. aureus Xen 30 was reduced by 41% and 50%, respectively. Reduction in biofilm formation was ascribed to copper released from the nanofibers. Copper-containing nanofibers may be incorporated into wound dressings.
authors.affiliation: Department of Microbiology, University of Stellenbosch, 7602 Matieland (Stellenbosch), South Africa.
authors.forename: Jayesh J
authors.initials: JJ
authors.lastname: Ahire
authors.affiliation: Department of Microbiology, University of Stellenbosch, 7602 Matieland (Stellenbosch), South Africa.
authors.forename: Melanie
authors.initials: M
authors.lastname: Hattingh
authors.affiliation: Department of Microbiology, University of Stellenbosch, 7602 Matieland (Stellenbosch), South Africa.
authors.forename: Deon P
authors.initials: DP
authors.lastname: Neveling
authors.affiliation: Department of Microbiology, University of Stellenbosch, 7602 Matieland (Stellenbosch), South Africa.
authors.forename: Leon M T
authors.initials: LM
authors.lastname: Dicks
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001458:Bandages; D018441:Biofilms / Q000254:growth & development*; D003300:Copper / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D013211:Staphylococcus aureus / Q000502:physiology*; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D003300:Copper