title: Protective Effect of a (Poly)phenol-Rich Extract Derived from Sweet Cherries Culls against Oxidative Cell Damage.
abstract: Oxidative stress is one of the key phenomena behind the most common types of chronic diseases. Therefore, the modulation of oxidative stress is an interesting target for acting either through prevention or as a therapeutic approach. In this work, a Portuguese variety of cherry (Saco Cherry) was processed in order to obtain a potent in vitro antioxidant phenolic-rich extract (Ch-PRE), which was further explored to evaluate its potential application as nutraceutical agent against cellular oxidative stress damage. Ch-PRE was mainly composed of anthocyanins, particularly cyanidin-3-rutinoside, cyanidin-3-glucoside, peonidin-3-glucoside and neochlorogenic acid, and exhibited a potent chemical antioxidant activity expressed by its oxygen radical absorbance capacity (ORAC) and hydroxyl radical averting capacity (HORAC) values. Ch-PRE also displayed effective intracellular radical scavenging properties in intestinal epithelial and neuronal cells challenged with oxidative stress but showed a different order of effectiveness regarding the modulation of endogenous antioxidant system. Ch-PRE could be an attractive candidate to formulate an agent for the prevention of oxidative stress-induced disorders such as intestinal inflammation disorders or with an appropriated delivery system for neurodegenerative diseases.
authors.affiliation: Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Avenida da República, Estação Agronómica Nacional, Oeiras 2780-157, Portugal. amatias@ibet.pt.|Instituto de Biologia Experimental e Tecnológica, Avenida da República, Quinta-do-Marquês, Estação Agronómica Nacional, Apartado 12, Oeiras 2781-901, Portugal. amatias@ibet.pt.|Instituto de Investigação do Medicamento (IMED), Faculdade de Farmácia da Universidade de Lisboa, Avenida das Forças Armadas, 1649-019 Lisboa, Portugal. amatias@ibet.pt.
authors.forename: Ana A
authors.initials: AA
authors.lastname: Matias
authors.affiliation: Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Avenida da República, Estação Agronómica Nacional, Oeiras 2780-157, Portugal. ritajoao.ramos@gmail.com.|Instituto de Biologia Experimental e Tecnológica, Avenida da República, Quinta-do-Marquês, Estação Agronómica Nacional, Apartado 12, Oeiras 2781-901, Portugal. ritajoao.ramos@gmail.com.
authors.forename: Rita
authors.initials: R
authors.lastname: Rosado-Ramos
authors.affiliation: Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Avenida da República, Estação Agronómica Nacional, Oeiras 2780-157, Portugal. sara.nunes88@gmail.com.|Instituto de Biologia Experimental e Tecnológica, Avenida da República, Quinta-do-Marquês, Estação Agronómica Nacional, Apartado 12, Oeiras 2781-901, Portugal. sara.nunes88@gmail.com.
authors.forename: Sara L
authors.initials: SL
authors.lastname: Nunes
authors.affiliation: Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Avenida da República, Estação Agronómica Nacional, Oeiras 2780-157, Portugal. inesf@itqb.unl.pt.
authors.forename: Inês
authors.initials: I
authors.lastname: Figueira
authors.affiliation: Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Avenida da República, Estação Agronómica Nacional, Oeiras 2780-157, Portugal. tserra@itqb.unl.pt.|Instituto de Biologia Experimental e Tecnológica, Avenida da República, Quinta-do-Marquês, Estação Agronómica Nacional, Apartado 12, Oeiras 2781-901, Portugal. tserra@itqb.unl.pt.
authors.forename: Ana Teresa
authors.initials: AT
authors.lastname: Serra
authors.affiliation: Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Avenida da República, Estação Agronómica Nacional, Oeiras 2780-157, Portugal. mrbronze@itqb.unl.pt.|Instituto de Biologia Experimental e Tecnológica, Avenida da República, Quinta-do-Marquês, Estação Agronómica Nacional, Apartado 12, Oeiras 2781-901, Portugal. mrbronze@itqb.unl.pt.|Instituto de Investigação do Medicamento (IMED), Faculdade de Farmácia da Universidade de Lisboa, Avenida das Forças Armadas, 1649-019 Lisboa, Portugal. mrbronze@itqb.unl.pt.
authors.forename: Maria R
authors.initials: MR
authors.lastname: Bronze
authors.affiliation: Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Avenida da República, Estação Agronómica Nacional, Oeiras 2780-157, Portugal. csantos@itqb.unl.pt.|Instituto de Biologia Experimental e Tecnológica, Avenida da República, Quinta-do-Marquês, Estação Agronómica Nacional, Apartado 12, Oeiras 2781-901, Portugal. csantos@itqb.unl.pt.
authors.forename: Claúdia N
authors.initials: CN
authors.lastname: Santos
authors.affiliation: Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Avenida da República, Estação Agronómica Nacional, Oeiras 2780-157, Portugal. cduarte@ibet.pt.|Instituto de Biologia Experimental e Tecnológica, Avenida da República, Quinta-do-Marquês, Estação Agronómica Nacional, Apartado 12, Oeiras 2781-901, Portugal. cduarte@ibet.pt.
authors.forename: Catarina M M
authors.initials: CM
authors.lastname: Duarte
mesh_terms: D000872:Anthocyanins / Q000737:chemistry* / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D018938:Caco-2 Cells; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D019636:Neurodegenerative Diseases / Q000188:drug therapy / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D000072257:Oxygen Radical Absorbance Capacity; D010636:Phenols / Q000737:chemistry* / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry; D000068220:Prunus avium / Q000737:chemistry
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts
keywords: Saco cherry; antioxidant; intestinal epithelium; neurodegeneration; oxidative stress; polyphenols