title: Genome-wide profiling of RNA polymerase transcription at nucleotide resolution in human cells with native elongating transcript sequencing.
abstract: Many features of how gene transcription occurs in human cells remain unclear, mainly because of a lack of quantitative approaches to follow genome transcription with nucleotide precision in vivo. Here we present a robust genome-wide approach for studying RNA polymerase II (Pol II)-mediated transcription in human cells at single-nucleotide resolution by native elongating transcript sequencing (NET-seq). Elongating RNA polymerase and the associated nascent RNA are prepared by cell fractionation, avoiding immunoprecipitation or RNA labeling. The 3' ends of nascent RNAs are captured through barcode linker ligation and converted into a DNA sequencing library. The identity and abundance of the 3' ends are determined by high-throughput sequencing, which reveals the exact genomic locations of Pol II. Human NET-seq can be applied to the study of the full spectrum of Pol II transcriptional activities, including the production of unstable RNAs and transcriptional pausing. By using the protocol described here, a NET-seq library can be obtained from human cells in 5 d.
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Andreas
authors.initials: A
authors.lastname: Mayer
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: L Stirling
authors.initials: LS
authors.lastname: Churchman
mesh_terms: D002458:Cell Fractionation; D020869:Gene Expression Profiling / Q000379:methods*; D015723:Gene Library; D006367:HeLa Cells; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012319:RNA Polymerase II / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
chemical_list: D012313:RNA; D012319:RNA Polymerase II