title: Correlative light and electron microscopy methods for the study of virus-cell interactions.
abstract: Electron microscopy (EM) is an invaluable tool to study the interactions of viruses with cells, and the ultrastructural changes induced in host cells by virus infection. Light microscopy (LM) is a complementary tool with the potential to locate rare events, label specific components, and obtain dynamic information. The combination of LM and EM in correlative light and electron microscopy (CLEM) is particularly powerful. It can be used to complement a static EM image with dynamic data from live imaging, identify the ultrastructure observed in LM, or, conversely, provide molecular specificity data for a known ultrastructure. Here, we describe methods and strategies for CLEM, discuss their advantages and limitations, and review applications of CLEM to study virus-host interactions.
authors.affiliation: Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: Yury S
authors.initials: YS
authors.lastname: Bykov
authors.affiliation: Department of Infectious Diseases, Molecular Virology, Heidelberg University, Germany.
authors.forename: Mirko
authors.identifier: 0000-0003-1786-4211
authors.initials: M
authors.lastname: Cortese
authors.affiliation: Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: John A G
authors.initials: JA
authors.lastname: Briggs
authors.affiliation: Department of Infectious Diseases, Molecular Virology, Heidelberg University, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Bartenschlager
mesh_terms: D000818:Animals; D002477:Cells / Q000648:ultrastructure* / Q000821:virology*; D054884:Host-Pathogen Interactions; D006801:Humans; D008854:Microscopy, Electron / Q000379:methods*; D014771:Virion / Q000648:ultrastructure; D014780:Viruses / Q000648:ultrastructure*
keywords: correlative light and electron microscopy; electron microscopy; virus-host interaction