title: Metagenome changes in the biogas producing community during anaerobic digestion of rice straw.
abstract: The present investigation was undertaken to study the microbial community succession in a sour and healthy digester. Ion torrent next-generation sequencing (NGS)-based metagenomic approach indicated abundance of hydrolytic bacteria and exclusion of methanogens and syntrophic bacteria in sour digester. Functional gene analysis revealed higher abundance of enzymes involved in acidogenesis and lower abundance of enzymes associated with methanogenesis like Methyl coenzyme M-reductase, F420 dependent reductase and Formylmethanofuran dehydrogenase in sour digester. Increased abundance of methanogens (Methanomicrobia) and genes involved in methanogenesis was observed in the restored/healthy digester highlighting revival of pH sensitive methanogenic community.
authors.affiliation: Bioenergy Group, MACS Agharkar Research Institute, Pune, India.
authors.forename: Soham D
authors.initials: SD
authors.lastname: Pore
authors.affiliation: Bioenergy Group, MACS Agharkar Research Institute, Pune, India; MCE Society's Abeda Inamdar Senior College of Arts, Science and Commerce, Pune, India.
authors.forename: Deepa
authors.initials: D
authors.lastname: Shetty
authors.affiliation: Bioenergy Group, MACS Agharkar Research Institute, Pune, India.
authors.forename: Preeti
authors.initials: P
authors.lastname: Arora
authors.affiliation: Bioenergy Group, MACS Agharkar Research Institute, Pune, India.
authors.forename: Sneha
authors.initials: S
authors.lastname: Maheshwari
authors.affiliation: Bioenergy Group, MACS Agharkar Research Institute, Pune, India. Electronic address: pkdhakephalkar@aripune.org.
authors.forename: Prashant K
authors.initials: PK
authors.lastname: Dhakephalkar
mesh_terms: D000693:Anaerobiosis; D001419:Bacteria / Q000235:genetics; D056804:Biofuels / Q000382:microbiology*; D019605:Euryarchaeota / Q000235:genetics; D005232:Fatty Acids, Volatile / Q000378:metabolism; D059014:High-Throughput Nucleotide Sequencing; D054892:Metagenome; D056186:Metagenomics; D008697:Methane / Q000378:metabolism*; D059013:Microbial Consortia; D012275:Oryza / Q000382:microbiology*; D010088:Oxidoreductases / Q000235:genetics
chemical_list: D056804:Biofuels; D005232:Fatty Acids, Volatile; D010088:Oxidoreductases; C021572:methyl coenzyme M reductase; D008697:Methane
keywords: Acidogenesis; Healthy digester; Metagenomics; Methanogenesis; Sour digester