title: [Gluten--mechanisms of intolerance, symptoms and treatment possibilities of IgE-related allergy for gluten in the light of actual clinical and immunological studies].
abstract: Gluten is the product of a chemical bond of wheat prolamin proteins (glia- dins and glutenins) in an aqueous me- dium. IgE mediated gluten allergy can be induced either by gluten as an in- gredient in foods or wheat prolamines present in the air. The aim of the study was clinical analysis of 13 patients, who demonstrated elevated levels of gluten specific IgE and identification of the most allergenic protein fractions from several samples of wheat using serum of examined subjects. Clinical analysis showed the occupational allergy to gluten in the form of rhinitis, asthma and airborne dermatistis in 9 subjects, whose symptoms disappeared during isolation from occupational exposure despite the use of a normal diet. In case of 4 patients with severe forms of chronic urticaria and atopic dermatitis, who are also allergic to grass pollen at the same time, the introduction of a gluten-free diet resulted in improvement of health conditions. The study of wheat protein fractions revealed a significant polymorphism dependent on the wheat sample. In the protein fractions, low and high molecular glutenin fractions, and alpha, beta, gamma, and omega-gliadins were separated. It has been shown that the strongest immunogenic effect causes omega-5 gliadin fraction. The removal of this fraction resulted in reduction of skin reactivity evaluated by skin prick test in the studied patients.
authors.forename: Krystyna
authors.initials: K
authors.lastname: Obtułowicz
authors.forename: Jacek
authors.initials: J
authors.lastname: Waga
authors.forename: Wojciech
authors.initials: W
authors.lastname: Dyga
mesh_terms: D000328:Adult; D001249:Asthma / Q000276:immunology / Q000628:therapy; D002675:Child, Preschool; D017449:Dermatitis, Allergic Contact / Q000276:immunology / Q000628:therapy; D005260:Female; D005983:Glutens / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood*; D008297:Male; D008875:Middle Aged; D065631:Rhinitis, Allergic / Q000276:immunology / Q000628:therapy; D012882:Skin Tests; D014581:Urticaria / Q000276:immunology / Q000628:therapy; D021182:Wheat Hypersensitivity / Q000276:immunology* / Q000378:metabolism / Q000628:therapy; D055815:Young Adult
chemical_list: D007073:Immunoglobulin E; D005983:Glutens