title: Substrate specificity of proteolytic activity in the testes fluid and seminal plasma of the common carp Cyprinus carpio.
abstract: Substrate specificity in the seminal plasma and testes fluids of the common carp Cyprinus carpio was determined using gelatin, casein, albumin and haemoglobin. Proteolytic profiles of the testes and seminal plasma were compared. Different ranges of pH (5·5-9·5) and temperature (4-37° C) were used during incubations of seminal plasma proteinases. Differences in proteolytic activity between testes and seminal plasma may reflect specific functions of the testes and sperm ducts in semen production. Seminal plasma metalloproteinases were characterized by higher substrate specificity than were serine proteinases. Zymography optimization for seminal plasma indicated that pH 7·5 and 22° C were the optimal conditions for gel incubations.
authors.affiliation: Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748, Olsztyn, Poland.
authors.forename: B I
authors.initials: BI
authors.lastname: Cejko
authors.affiliation: Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748, Olsztyn, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Słowińska
authors.affiliation: Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748, Olsztyn, Poland.
authors.forename: S
authors.initials: S
authors.lastname: Judycka
authors.affiliation: Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748, Olsztyn, Poland.
authors.forename: R K
authors.initials: RK
authors.lastname: Kowalski
mesh_terms: D000818:Animals; D001826:Body Fluids; D002347:Carps / Q000378:metabolism*; D010450:Endopeptidases; D006863:Hydrogen-Ion Concentration; D008297:Male; D010447:Peptide Hydrolases / Q000378:metabolism*; D012661:Semen / Q000201:enzymology*; D013094:Spermatozoa; D013379:Substrate Specificity; D013696:Temperature; D013737:Testis / Q000201:enzymology*
chemical_list: D010450:Endopeptidases; D010447:Peptide Hydrolases
keywords: casein; fish; gelatin; pH; proteases; reproductive tract