title: Towards "bionic" proteins: replacement of continuous sequences from HIF-1α with proteomimetics to create functional p300 binding HIF-1α mimics.
abstract: Using the HIF-1α transcription factor as a model, this manuscript illustrates how an extended sequence of α-amino acids in a polypeptide can be replaced with a non-natural topographical mimic of an α-helix comprised from an aromatic oligoamide. The resultant hybrid is capable of reproducing the molecular recognition profile of the p300 binding sequence of HIF-1α from which it is derived.
authors.affiliation: School of Chemistry, University of Leeds, Woodhouse Lane, Leeds, LS29JT, UK and Astbury Centre for Structural Molecular Biology, University of Leeds, Woodhouse Lane, Leeds, LS29JT, UK. a.j.wilson@leeds.ac.uk.
authors.forename: George M
authors.initials: GM
authors.lastname: Burslem
authors.affiliation: Astbury Centre for Structural Molecular Biology, University of Leeds, Woodhouse Lane, Leeds, LS29JT, UK. a.j.wilson@leeds.ac.uk and School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Woodhouse Lane, Leeds LS2 9JT, UK.
authors.forename: Hannah F
authors.initials: HF
authors.lastname: Kyle
authors.affiliation: Astbury Centre for Structural Molecular Biology, University of Leeds, Woodhouse Lane, Leeds, LS29JT, UK. a.j.wilson@leeds.ac.uk and School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Woodhouse Lane, Leeds LS2 9JT, UK and Discovery Sciences, AstraZeneca R&D, Alderley Park, Cheshire, SK10 4TG, UK.
authors.forename: Alexander L
authors.initials: AL
authors.lastname: Breeze
authors.affiliation: Astbury Centre for Structural Molecular Biology, University of Leeds, Woodhouse Lane, Leeds, LS29JT, UK. a.j.wilson@leeds.ac.uk and School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Woodhouse Lane, Leeds LS2 9JT, UK.
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Edwards
authors.affiliation: School of Chemistry, University of Leeds, Woodhouse Lane, Leeds, LS29JT, UK and Astbury Centre for Structural Molecular Biology, University of Leeds, Woodhouse Lane, Leeds, LS29JT, UK. a.j.wilson@leeds.ac.uk.
authors.forename: Adam
authors.initials: A
authors.lastname: Nelson
authors.affiliation: School of Chemistry, University of Leeds, Woodhouse Lane, Leeds, LS29JT, UK and Astbury Centre for Structural Molecular Biology, University of Leeds, Woodhouse Lane, Leeds, LS29JT, UK. a.j.wilson@leeds.ac.uk.
authors.forename: Stuart L
authors.initials: SL
authors.lastname: Warriner
authors.affiliation: School of Chemistry, University of Leeds, Woodhouse Lane, Leeds, LS29JT, UK and Astbury Centre for Structural Molecular Biology, University of Leeds, Woodhouse Lane, Leeds, LS29JT, UK. a.j.wilson@leeds.ac.uk.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Wilson
mesh_terms: D000577:Amides / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D040761:Biomimetic Materials / Q000737:chemistry* / Q000378:metabolism; D001701:Bionics; D050881:E1A-Associated p300 Protein / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D006841:Hydrocarbons, Aromatic / Q000737:chemistry* / Q000378:metabolism; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D000072756:Protein Conformation, alpha-Helical
chemical_list: D000577:Amides; D006841:Hydrocarbons, Aromatic; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D010455:Peptides; D050881:E1A-Associated p300 Protein; C494360:EP300 protein, human