title: Prevention of Cutaneous Penetration and CD1c+ Uptake of Pollen Allergens by a Barrier-Enhancing Formulation.
abstract: Recent studies have shown that pollen proteins can penetrate the impaired skin barrier of atopic patients and exacerbate their disease. In the presented study the effect of a topically applied barrier-enhancing formulation was investigated for its preventive effect on the uptake of pollen allergens into CD1c+ epidermal cells. The pollen proteins were fluorescence labelled and applied on barrier-disrupted excised human skin. CD1c+ cells were selected after magnetic cell sorting and analysed using laser scanning microscopy. In untreated disrupted skin, 81% of the CD1c+ cells contained the fluorescence-labelled pollen allergens. In formulation-pretreated skin only 12% of the CD1c+ cells showed an uptake of pollen allergens. These results encourage the treatment of atopic patients with barrier-enhancing formulations to reduce the impact of pollen on air-exposed skin areas and hence the exacerbation of cutaneous symptoms.
authors.affiliation: Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Venerology and Allergology, Charitx00E9; - Universitx00E4;tsmedizin Berlin, Berlin, Germany.
authors.forename: Martina Claudia
authors.initials: MC
authors.lastname: Meinke
authors.forename: Sabine
authors.initials: S
authors.lastname: Schanzer
authors.forename: Heike
authors.initials: H
authors.lastname: Richter
authors.forename: Frank
authors.initials: F
authors.lastname: Rippke
authors.forename: Alexander
authors.initials: A
authors.lastname: Filbry
authors.forename: Kerstin
authors.initials: K
authors.lastname: Bohnsack
authors.forename: Alexa
authors.initials: A
authors.lastname: Patzelt
authors.forename: Jürgen
authors.initials: J
authors.lastname: Lademann
mesh_terms: D000279:Administration, Cutaneous; D000485:Allergens / Q000008:administration & dosage / Q000378:metabolism*; D018949:Antigens, CD1 / Q000008:administration & dosage / Q000378:metabolism*; D004339:Drug Compounding; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000378:metabolism*; D006023:Glycoproteins / Q000008:administration & dosage / Q000378:metabolism*; D006801:Humans; D008055:Lipids / Q000008:administration & dosage / Q000493:pharmacokinetics; D009823:Ointment Bases / Q000008:administration & dosage / Q000378:metabolism*; D011058:Pollen / Q000378:metabolism*
chemical_list: D000485:Allergens; D018949:Antigens, CD1; C437944:CD1C protein, human; D005456:Fluorescent Dyes; D006023:Glycoproteins; D008055:Lipids; D009823:Ointment Bases; C039909:eucerin