title: Changes in microbial community structures due to varying operational conditions in the anaerobic digestion of oxytetracycline-medicated cow manure.
abstract: Management of manure containing veterinary antibiotics is a major concern in anaerobic treatment systems because of their possible adverse effects on microbial communities. Therefore, the aim of study was to investigate how oxytetracycline (OTC) influences bacteria and acetoclastic and hydrogenotrophic methanogens under varying operational conditions in OTC-medicated and non-medicated anaerobic cow manure digesters. Concentrations of OTC and its metabolites throughout the anaerobic digestion were determined using ultraviolet-high-performance liquid chromatography (UV-HPLC) and tandem liquid chromatography-mass spectrometry (LC/MS/MS), respectively. Fluorescent in situ hybridization, denaturing gradient gel electrophoresis, cloning, and sequencing analyses were used to monitor changes in microbial community structures. According to the results of analytical and molecular approaches, operating conditions highly influence active microbial community dynamics and associate with biogas production and elimination of OTC and its metabolites during anaerobic digestion of cow manure in the presence of an average initial concentration of 2.2 mg OTC/L. The impact of operating conditions has a drastic effect on acetoclastic methanogens than hydrogenotrophic methanogens and bacteria.
authors.affiliation: Institute of Environmental Sciences, Boğaziçi University, Bebek, 34342, Istanbul, Turkey.
authors.forename: Gokhan
authors.initials: G
authors.lastname: Turker
authors.affiliation: Department of Environmental Engineering, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey. sevcan_aydn@hotmail.com.
authors.forename: Sevcan
authors.initials: S
authors.lastname: Aydin
authors.affiliation: Institute of Environmental Sciences, Boğaziçi University, Bebek, 34342, Istanbul, Turkey.
authors.forename: Çağrı
authors.initials: Ç
authors.lastname: Akyol
authors.affiliation: Institute of Environmental Sciences, Boğaziçi University, Bebek, 34342, Istanbul, Turkey.
authors.forename: Orhan
authors.initials: O
authors.lastname: Yenigun
authors.affiliation: Department of Environmental Engineering, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey.
authors.forename: Orhan
authors.initials: O
authors.lastname: Ince
authors.affiliation: Institute of Environmental Sciences, Boğaziçi University, Bebek, 34342, Istanbul, Turkey.
authors.forename: Bahar
authors.initials: B
authors.lastname: Ince
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D001673:Biodegradation, Environmental; D056804:Biofuels / Q000382:microbiology; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D002417:Cattle; D019641:DNA, Archaeal / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D008372:Manure / Q000382:microbiology*; D017012:Methanobacteriales / Q000145:classification; D017018:Methanomicrobiales / Q000145:classification; D017019:Methanosarcinaceae / Q000145:classification; D010118:Oxytetracycline / Q000737:chemistry*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D053719:Tandem Mass Spectrometry
chemical_list: D056804:Biofuels; D019641:DNA, Archaeal; D004269:DNA, Bacterial; D008372:Manure; D012336:RNA, Ribosomal, 16S; D010118:Oxytetracycline
keywords: Anaerobic digestion; Biogas; Cow manure; Microbial community; Oxytetracycline