title: Adipose-Derived Stem Cells as a Tool for Dental Implant Osseointegration: an Experimental Study in the Dog.
abstract: The biological interaction between the jaw bones and dental implant is fundamental for the long-term success of dental implant placement. Nevertheless, the insufficient bone volume remains a major clinical problem, especially in case of immediate dental implant. Using a canine model, the present study proves the regenerative potential of adipose- derived stem cells (ADSCs) to repair peri-implant bone defects occurring in immediate dental implant placement. In six labradors, all mandibular premolars and the first molars were extracted bilaterally and three months later dental implants were installed with a marginal gap. The marginal defects were filled with hydroxyapatite (HA)-based scaffolds previously seeded with ADSCs. After one month of healing, specimens were prepared for histological and histomorphometric evaluations. Histological analyses of ground sections show that ADSCs significantly increase bone regeneration. Several new vessels, osteoblasts and new bone matrix were detected. By contrast, no inflammatory cells have been revealed. ADSCs could be used to accelerate bone healing in peri- implant defects in case of immediate dental implant placement.
authors.affiliation: Department of Neurosciences, University of Padua, Padua, Italy.; Faculty of Dentistry, University of Medical Science, La Habana, Cuba.
authors.forename: Eriberto
authors.initials: E
authors.lastname: Bressan
authors.affiliation: Faculty of Dentistry, University of Medical Science, La Habana, Cuba.; Oral Surgery Division, ARDEC, Ariminum Odontologica, Rimini, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Botticelli
authors.affiliation: Department of Neurosciences, University of Padua, Padua, Italy.; Faculty of Dentistry, University of Medical Science, La Habana, Cuba.
authors.forename: Stefano
authors.initials: S
authors.lastname: Sivolella
authors.affiliation: Faculty of Dentistry, University of Medical Science, La Habana, Cuba.
authors.forename: Franco
authors.initials: F
authors.lastname: Bengazi
authors.affiliation: Department of Neurosciences, University of Padua, Padua, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Guazzo
authors.affiliation: Department of Neurosciences, University of Padua, Padua, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Sbricoli
authors.affiliation: Department of Neurosciences, University of Padua, Padua, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Ricci
authors.affiliation: Department of Neurosciences, University of Padua, Padua, Italy.
authors.forename: Letizia
authors.initials: L
authors.lastname: Ferroni
authors.affiliation: Department of Biomedical Sciences, University of Padua, Via Bassi 58, 35100 Padua, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Gardin
authors.affiliation: Faculty of Dentistry, University of Medical Science, La Habana, Cuba.
authors.forename: Joaquin Urbizo
authors.initials: JU
authors.lastname: Velez
authors.affiliation: Department of Biomedical Sciences, University of Padua, Via Bassi 58, 35100 Padua, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Zavan
keywords: Adipose-derived stem cells; alveolar bone loss; bone regeneration; hydroxyapatite; immediate dental implant; peri-implant bone defect