title: A novel stoichiometries methodology to quantify functional microorganisms in simultaneous (partial) nitrification-endogenous denitrification and phosphorus removal (SNEDPR).
abstract: Although efficient removal of carbon (C), nitrogen (N) and phosphorous (P) from wastewater with low C/N ratio was achieved in anaerobic/aerobic simultaneous nitrification-endogenous denitrification and phosphorus removal (SNEDPR) systems, the removal pathways and metabolic transformations in this complex system are unclear. This work targeted at developing the stoichiometric models for denitrifying glycogen organisms (DGAOs) via nitrite and nitrate (DGAONi and DGAONa), and demonstrating a novel methodology to quantify diverse functional microorganisms (e.g. ammonia and nitrite oxidizing bacteria, aerobic phosphorus accumulating organisms (APAOs), denitrifying PAOs (DPAOs) and aerobic GAOs (AGAOs)) for the removal of C, N and P. The results showed that the anaerobic intracellular carbon storage (CODintra) was mainly accomplished by GAOs, and PAOs were only responsible for about 40% of CODintra through a stable P release. At the aerobic stage, 84.9% of P was removed by APAOs with 15.1% left by DPAOs, while 64.6% of N was removed by DGAOs (45.8% by DGAONi and 18.8% by DGAONa) with 18.1% by DPAOs and 17.3% by bacterial growth. High proportion of N removal via nitrite (partial nitrification-endogenous denitrification) (71%) saved 7.3% aeration and 38% intracellular carbon demand. However, AGAOs still activated well at the aerobic intercellular carbon consumption, which limited the further improvement of N removal efficiency. By elucidating the nutrient removal pathways among diverse functional microorganisms, the methodology developed in this study could accelerate the nutrient removal in the SNEDPR process.
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China. Electronic address: wsy@bjut.edu.cn.
authors.forename: Shuying
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Ji
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Xian
authors.initials: X
authors.lastname: Dai
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China; Department of Civil and Environmental Engineering, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: Baikun
authors.initials: B
authors.lastname: Li
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Yongzhen
authors.initials: Y
authors.lastname: Peng
mesh_terms: D019149:Bioreactors / Q000382:microbiology; D058440:Denitrification; D058465:Nitrification; D009584:Nitrogen / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism; D014865:Waste Disposal, Fluid
chemical_list: D010758:Phosphorus; D009584:Nitrogen
keywords: Denitrifying glycogen accumulating organisms (DGAOs); Partial nitrification (PN); Phosphorous accumulating organisms (PAOs); Simultaneous nitrification-endogenous denitrification and phosphorus removal (SNEDPR); Stoichiometric model