title: Kinetics and avidity of anti-Toxocara antibodies (IgG) in rabbits experimentally infected with Toxocara canis.
abstract: An evaluation was made of the kinetics and avidity of anti-Toxocara antibodies (IgG) in rabbits experimentally infected with embryonated Toxocara canis eggs. Seventeen four month old New Zealand White rabbits were distributed into two groups. In the experimental group, twelve rabbits were infected orally with 1,000 embryonated T. canis eggs. A second group (n = 5), uninfected, was used as a control. Serum samples were collected for analysis on days 7, 14, 21, 28 and 60 post-infection (DPI). An indirect ELISA test was performed to evaluate the reactivity index (RI) of IgG anti-T. canis antibodies and to calculate the avidity index (AI). The animals showed seroconversion from the 14th DPI, with high AI (over 50%) except for one animal, which presented an intermediate AI. At 60 DPI, all the animals were seropositive and maintained a high AI. The data indicated that specific IgG antibodies formed early (14 DPI) in rabbits infected with T. canis, with a high avidity index that persisted throughout the course of the infection.
authors.affiliation: Programa de Pós-Graduação em Ciência Animal, Universidade do Oeste Paulista, Presidente Prudente, SP, Brasil.
authors.forename: Lundia Luara Cavalcante
authors.initials: LL
authors.lastname: Bin
authors.affiliation: Programa de Pós-Graduação em Ciência Animal, Universidade do Oeste Paulista, Presidente Prudente, SP, Brasil.
authors.forename: Vamilton Alvares
authors.initials: VA
authors.lastname: Santarém
authors.affiliation: Programa de Pós-Graduação em Ciência Animal, Universidade do Oeste Paulista, Presidente Prudente, SP, Brasil.
authors.forename: Cecília Braga
authors.initials: CB
authors.lastname: Laposy
authors.affiliation: Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: Guita
authors.initials: G
authors.lastname: Rubinsky-Elefant
authors.affiliation: Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: William Henry
authors.initials: WH
authors.lastname: Roldán
authors.affiliation: Programa de Pós-Graduação em Ciência Animal, Universidade do Oeste Paulista, Presidente Prudente, SP, Brasil.
authors.forename: Rogério
authors.initials: R
authors.lastname: Giuffrida
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth; D000915:Antibody Affinity; D007074:Immunoglobulin G / Q000276:immunology*; D007700:Kinetics; D010063:Ovum / Q000276:immunology; D011817:Rabbits; D017166:Toxocara canis / Q000276:immunology*; D014120:Toxocariasis / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D007074:Immunoglobulin G