title: Tertiary Dentin Formation after Indirect Pulp Capping Using Protein CPNE7.
abstract: If there is a partial loss of dentin, the exposed dentinal surface should be protected by an indirect pulp capping (IPC) procedure to preserve pulp vitality and prevent symptoms of dentin hypersensitivity. In our previous study, copine7 (CPNE7) induced odontoblast differentiation in vitro and promoted dentin formation in vivo. The aim of this study was to investigate the possibility of IPC therapy using the CPNE7 protein at the exposed dentinal surface and the resulting effects on tertiary dentin formation in a beagle model. CPNE7 promoted mineralization of odontoblasts and had high calcium ion-binding capacity. The in vivo IPC model with canine teeth showed that regeneration of physiologic reactionary dentin with dentinal tubule structures was clearly observed beneath the remaining dentin in the CPNE7 group, whereas irregular features of reparative dentin were generated in the mineral trioxide aggregate (MTA) group. The CPNE7+MTA group also showed typical reactionary dentin without reparative dentin, showing synergistic effects of CPNE7 with MTA. A scanning electron microscopy analysis showed that dentinal tubules beneath the original dentin were occluded by the deposition of peritubular dentin in the CPNE7 and CPNE7+MTA groups, whereas those in the control group were opened. Therefore, CPNE7 may be able to serve as a novel IPC material and improve symptoms of dentin hypersensitivity.
authors.affiliation: Department of Oral Histology/Developmental Biology, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea.
authors.forename: H W
authors.initials: HW
authors.lastname: Choung
authors.affiliation: Department of Oral Histology/Developmental Biology, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea.
authors.forename: D S
authors.initials: DS
authors.lastname: Lee
authors.affiliation: Department of Oral Histology/Developmental Biology, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea.
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Conservative Dentistry, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea.
authors.forename: W J
authors.initials: WJ
authors.lastname: Shon
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea.
authors.forename: Jong-Ho
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea.
authors.forename: Y
authors.initials: Y
authors.lastname: Ku
authors.affiliation: Department of Oral Histology/Developmental Biology, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea jcapark@snu.ac.kr.
authors.forename: J C
authors.initials: JC
authors.lastname: Park
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003785:Dental Pulp Capping / Q000379:methods*; D003804:Dentin / Q000187:drug effects / Q000254:growth & development*; D004285:Dogs; D006801:Humans; D008565:Membrane Proteins / Q000627:therapeutic use*; D009804:Odontoblasts / Q000187:drug effects / Q000502:physiology; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C400933:CPNE7 protein, human; D008565:Membrane Proteins
keywords: biomaterial(s); dentinogenesis; development; epithelial-mesenchymal interaction; mineralized tissue; regeneration