title: Common molecular mechanism of the hepatic lesion and the cardiac parasympathetic regulation in chronic hepatitis C infection: a critical role for the muscarinic receptor type 3.
abstract: BACKGROUND
  The pathophysiological overlapping between Sjorgen's Syndrome (SS) and HCV, presence of anti- muscarinic receptor type 3 (M3R) antibodies in SS, the role that M3R plays in the regulation of the heart rate, has led to the assumption that cardiovagal dysfunction in HCV patients is caused by anti-M3R antibodies elicited by HCV proteins or by their direct interaction with M3R.
  
  
  RESULTS
  To identify HCV protein which possibly is crossreactive with M3R or which binds to this receptor, we performed the Informational Spectrum Method (ISM) analysis of the HCV proteome. This analysis revealed that NS5A protein represents the most probable interactor of M3R or that this viral protein could elicit antibodies which modulate function of this receptor. Further detailed structure/function analysis of NS5A and M3R performed by the ISM method extended with other Digital Signal processing (DSP) approaches revealed domains of these proteins which participate in their crossreactivity or in their direct interaction, representing promising diagnostic and therapeutic targets.
  
  
  CONCLUSIONS
  Application of the ISM with other compatible bioinformatics methods offers new perspectives for identifying diagnostic and therapeutic targets for complicated forms of HCV and other viral infections. We show how the electron-ion interaction potential (EIIP) amino-acid scale used in the ISM combined with a robust, high performance hydrophobicity scale can provide new insights for understanding protein structure/function and protein-protein interactions.
authors.affiliation: Institute of Nuclear Sciences Vinča, University of Belgrade, Center for Multidisciplinary Research, PO Box 522, Belgrade, Serbia.
authors.forename: Sanja
authors.initials: S
authors.lastname: Glišić
authors.affiliation: Benchmark Electronics, Bradford Dr., Huntsville, AL, 35805, USA.
authors.forename: David P
authors.initials: DP
authors.lastname: Cavanaugh
authors.affiliation: Chemical and Materials Engineering, University of Alabama Huntsville, Huntsville, AL, 35899, USA.
authors.forename: Krishnan K
authors.initials: KK
authors.lastname: Chittur
authors.affiliation: Institute of Nuclear Sciences Vinča, University of Belgrade, Center for Multidisciplinary Research, PO Box 522, Belgrade, Serbia.
authors.forename: Milan
authors.initials: M
authors.lastname: Sencanski
authors.affiliation: Institute of Nuclear Sciences Vinča, University of Belgrade, Center for Multidisciplinary Research, PO Box 522, Belgrade, Serbia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Perovic
authors.affiliation: Institute of Nuclear Sciences Vinča, University of Belgrade, Laboratory of Radiobiology and Molecular Genetics, PO Box 522, 11000, Belgrade, Serbia. tijanabojic@vinca.rs.
authors.forename: Tijana
authors.initials: T
authors.lastname: Bojić
mesh_terms: D000465:Algorithms; D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism; D001323:Autoantibodies; D001665:Binding Sites; D016174:Hepacivirus / Q000302:isolation & purification / Q000378:metabolism; D019698:Hepatitis C, Chronic / Q000378:metabolism / Q000473:pathology / Q000821:virology; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008297:Male; D062105:Molecular Docking Simulation; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D043587:Receptor, Muscarinic M3 / Q000737:chemistry / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000378:metabolism; D012324:RNA-Dependent RNA Polymerase
chemical_list: D000596:Amino Acids; D001323:Autoantibodies; D043587:Receptor, Muscarinic M3; D017361:Viral Nonstructural Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase
keywords: Autonomic nervous system; Cross-reactivity; EIIP; Hepatitis C; Hydrophobicity; Informational Spectrum Method; Muscarinic receptor type 3