title: Cytotoxicity induced by cypermethrin in Human Neuroblastoma Cell Line SH-SY5Y.
abstract: The purpose of this study was to evaluate the cytotoxic potential of Cypermethrin (CM) on cultured human Neuroblastoma SH-SY5Y cells. SH-SY5Y cells were treated with CM at 0-200µM for 24, 48, and 72 h, in vitro. It was found that CM induced the cell death of Neuroblastoma cells in a dose- and time-dependent manner, as shown by LDH assays. Next, some aspects of the process of cell death triggered by CM in the human SH-SY5Y cell line were investigated. It was revealed that the pan-caspase inhibitor Q-VD-OPh, sensitizes SH-SY5Y cells to necroptosis caused by CM. Furthermore, signal transduction inhibitors PD98059, SL-327, SB202190, SP600125 failed to attenuate the effect of the pesticide. Finally, it was shown that inhibition of TNF-a by Pomalidomide (PLD) caused statistically significant reduction in CM-induced cytotoxicity. Overall, the data obtained suggest that CM induces neurotoxicity in SH-SY5Y cells by necroptosis.
authors.affiliation: Department of Physiopathology, Institute of Rural Health, Lublin, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Raszewski
authors.affiliation: Department of Medical Biology, Institute of Rural Health, Lublin, Poland.
authors.forename: Marta Kinga
authors.initials: MK
authors.lastname: Lemieszek
authors.affiliation: Department of Physiopathology, Institute of Rural Health, Lublin, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Łukawski
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007306:Insecticides / Q000008:administration & dosage / Q000633:toxicity*; D009447:Neuroblastoma / Q000473:pathology*; D011722:Pyrethrins / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D007306:Insecticides; D011722:Pyrethrins; C017160:cypermethrin