title: Diagnostic and prognostic role of serum protein peak at 6449 m/z in gastric adenocarcinoma based on mass spectrometry.
abstract: BACKGROUND
  Gastric cancer (GC) is a highly aggressive cancer type associated with significant mortality owing to delayed diagnosis and non-specific symptoms observed in the early stages. Therefore, identification of novel specific GC serum biomarkers for screening purposes is an urgent clinical requirement.
  
  
  METHODS
  This study recruited a total of 432 serum samples from 296 GC patients split into the mining and testing sets. We aimed to screen for reliable protein biomarkers from matched serum samples based on mass spectrometry, followed by comparison with three representative conventional markers using receiver operating characteristic and survival curve analyses to ascertain their potential values as diagnostic and prognostic biomarkers for GC.
  
  
  RESULTS
  We identified an apoC-III fragment with confirmation in an independent test set from a second hospital. We found that the diagnostic ability of this fragment performed better than current standard GC diagnostic biomarkers both individually and in combination in distinguishing patients with GC from healthy individuals. Moreover, we found that this apoC-III protein fragment represents a more robust potential prognostic factor for GC than the three conventional markers.
  
  
  CONCLUSIONS
  In view of these findings, we suggest that apoC-III protein fragment is a novel diagnostic and prognostic biomarker, a complement to conventional biomarkers in detecting GC.
authors.affiliation: Department of Pediatric Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.|Institute of Clinical Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.
authors.forename: Dongjian
authors.initials: D
authors.lastname: Song
authors.affiliation: Department of Ultrasonography, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.
authors.forename: Lifang
authors.initials: L
authors.lastname: Yue
authors.affiliation: Department of Pediatric Surgery, Guiyang Maternal and Child Health Hospital, Guiyang, Guizhou 550003, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Pediatric Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.
authors.forename: Zechen
authors.initials: Z
authors.lastname: Yan
authors.affiliation: Department of Pediatric Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.
authors.forename: Yingzhong
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Department of Pediatric Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.
authors.forename: Heying
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Pediatric Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.
authors.forename: Qiuliang
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Pediatric Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.
authors.forename: Da
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Pediatric Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.
authors.forename: Ziqiang
authors.initials: Z
authors.lastname: Xia
authors.affiliation: Department of Pediatric Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Qin
authors.affiliation: Department of Pediatric Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Jia
authors.affiliation: Department of Pediatric Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yue
authors.affiliation: Institute of Cancer, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310000, China.
authors.forename: Jiekai
authors.initials: J
authors.lastname: Yu
authors.affiliation: Institute of Cancer, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310000, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Proteomic Platform, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Fuquan
authors.initials: F
authors.lastname: Yang
authors.affiliation: Department of Pediatric Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.
authors.forename: Jiaxiang
authors.initials: J
authors.lastname: Wang
mesh_terms: D000230:Adenocarcinoma / Q000097:blood* / Q000175:diagnosis* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008875:Middle Aged; D011379:Prognosis; D013274:Stomach Neoplasms / Q000097:blood* / Q000175:diagnosis* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D001798:Blood Proteins