title: Optimization of Process Parameters and Kinetic Model of Enzymatic Extraction of Polyphenols from Lonicerae Flos.
abstract: OBJECTIVE
  To optimize and verify the cellulase extraction of polyphenols from honeysuckle and provide a reference for enzymatic extracting polyphenols from honeysuckle.
  
  
  METHODS
  The uniform design was used According to Fick's first law and kinetic model, fitting analysis of the dynamic process of enzymatic extracting polyphenols was conducted.
  
  
  RESULTS
  The optimum enzymatic extraction parameters for polyphenols from honeysuckle are found to be 80% (v/v) of alcohol, 35:1 (mL/g) of liquid-solid ratio, 80°C of extraction temperature, 8.5 of pH, 6.0 mg of enzyme levels, and 130 min of extraction time. Under the optimal conditions, the extraction rate of polyphenols was 3.03%. The kinetic experiments indicated kinetic equation had a good linear relationship with t even under the conditions of different levels of enzyme and temperature, which means fitting curve tallies well with the experimental values.
  
  
  CONCLUSIONS
  The results of quantification showed that the results provide a reference for enzymatic extracting polyphenols from honeysuckle.
  
  
  CONCLUSIONS
  Lonicerae flos (Lonicera japonica Thunb.) is a material of traditional Chinese medicine and healthy drinks, of which active compounds mainly is polyphenols. At present, plant polyphenols are the hotspots centents of food, cosmetic and medicine, because it has strong bioactivity. Several traditional methods are available for the extraction of plant polyphenols including impregnation, solvent extraction, ultrasonic extraction, hot-water extraction, alkaline dilute alcohol or alkaline water extraction, microwave extraction and Supercritical CO2 extraction. But now, an increasing number of research on using cellulase to extract active ingredients from plants. Enzymatic method is widely used for enzyme have excellent properties of high reaction efficiency and specificity, moderate reaction conditions, shorter extraction time and easier to control, less damage to the active ingredient. At present, the enzymatic extraction of polyphenols from honeysuckle and dynamic had not been reported. In this study, using cellulase to extract polyphenols from honeysuckle is first applied. Moreover, uniform design was used to optimize process and kinetic model of extraction was established to analyze the characteristics of enzymatic extraction, in order to improve the yield of polyphenols from honeysuckle and make maximum use of Lonicerae flos, which provide references for industrial production.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China; College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, China.
authors.forename: Fansheng
authors.initials: F
authors.lastname: Kong
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, China.
authors.forename: Shujuan
authors.initials: S
authors.lastname: Yu
authors.affiliation: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, China.
authors.forename: Yongguang
authors.initials: Y
authors.lastname: Bi
authors.affiliation: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Huang
authors.affiliation: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, China.
authors.forename: Mengqian
authors.initials: M
authors.lastname: Huang
keywords: Cellulase; Lonicerae Flos; kinetic model; polyphenols; uniform design