title: Adsorbed plasma proteins modulate the effects of single-walled carbon nanotubes on neutrophils in blood.
abstract: Proteins adsorbed on a surface may affect the interaction of this surface with cells. Here, we studied the binding of human serum albumin (HSA), fibrinogen (FBG) and immunoglobulin G (IgG) to PEGylated single-walled carbon nanotubes (PEG-SWCNTs) and evaluated the impact of PEG-SWCNT treated by these proteins on neutrophils in whole blood samples. Measurements of adsorption parameters revealed tight binding of proteins to PEG-SWCNTs. AFM was employed to directly observe protein binding to sidewalls of PEG-SWCNTs. Fluorescein-labeled IgG was used to ascertain the stability of PEG-SWCNT-IgG complexes in plasma. In blood samples, all plasma proteins mitigated damage of neutrophils observed just after blood exposure to PEG-SWCNTs, while only treatment of PEG-SWCNTs with IgG resulted in dose- and time-dependent enhancement of CNT-induced neutrophil activation and in potentiation of oxidative stress. Our study demonstrates the ability of adsorbed plasma proteins to influence neutrophil response caused by PEG-SWCNTs in whole blood.
authors.affiliation: Research Institute for Physico-Chemical Medicine, FMBA, Moscow, Russia. Electronic address: irina.vlasova@yahoo.com.
authors.forename: Irina I
authors.initials: II
authors.lastname: Vlasova
authors.affiliation: Research Institute for Physico-Chemical Medicine, FMBA, Moscow, Russia.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Mikhalchik
authors.affiliation: Research Institute for Physico-Chemical Medicine, FMBA, Moscow, Russia.
authors.forename: Nikolay A
authors.initials: NA
authors.lastname: Barinov
authors.affiliation: Research Institute for Physico-Chemical Medicine, FMBA, Moscow, Russia; Research Institute of Experimental Medicine, Saint Petersburg, Russia.
authors.forename: Valeria A
authors.initials: VA
authors.lastname: Kostevich
authors.affiliation: Research Institute for Physico-Chemical Medicine, FMBA, Moscow, Russia.
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Smolina
authors.affiliation: Research Institute for Physico-Chemical Medicine, FMBA, Moscow, Russia.
authors.forename: Dmitry V
authors.initials: DV
authors.lastname: Klinov
authors.affiliation: Research Institute for Physico-Chemical Medicine, FMBA, Moscow, Russia; Research Institute of Experimental Medicine, Saint Petersburg, Russia; Saint Petersburg State University, Saint Petersburg, Russia.
authors.forename: Alexey V
authors.initials: AV
authors.lastname: Sokolov
mesh_terms: D000327:Adsorption; D001798:Blood Proteins / Q000502:physiology*; D006801:Humans; D037742:Nanotubes, Carbon; D009504:Neutrophils / Q000187:drug effects*; D011485:Protein Binding
chemical_list: D001798:Blood Proteins; D037742:Nanotubes, Carbon
keywords: Albumin; Fibrinogen; Immunoglobulin G; Myeloperoxidase; Neutrophil activation; PEGylated carbon nanotubes