title: Human mesenchymal stem cells are resistant to cytotoxic and genotoxic effects of cisplatin in vitro.
abstract: Mesenchymal stem cells (MSCs) are known for their important properties involving multilineage differentiation potential., trophic factor secretion and localization along various organs and tissues. On the dark side, MSCs play a distinguished role in tumor microenvironments by differentiating into tumor-associated fibroblasts or supporting tumor growth via distinct mechanisms. Cisplatin (CIS) is a drug widely applied in the treatment of a large number of cancers and is known for its cytotoxic and genotoxic effects, both in vitro and in vivo. Here we assessed the effects of CIS on MSCs and the ovarian cancer cell line OVCAR-3, by MTT and comet assays. Our results demonstrated the resistance of MSCs to cell death and DNA damage induction by CIS, which was not observed when OVCAR-3 cells were exposed to this drug.
authors.affiliation: Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Bruno Corrêa
authors.initials: BC
authors.lastname: Bellagamba
authors.affiliation: Universidade Luterana do Brasil, Canoas, RS, Brazil.
authors.forename: Bianca Regina Ribas de
authors.initials: BR
authors.lastname: Abreu
authors.affiliation: Universidade Luterana do Brasil, Canoas, RS, Brazil.
authors.forename: Ivana
authors.initials: I
authors.lastname: Grivicich
authors.affiliation: Complexo Hospitalar Santa Casa de Porto Alegre, Porto Alegre, RS, Brazil.
authors.forename: Carolina Franke
authors.initials: CF
authors.lastname: Markarian
authors.affiliation: Complexo Hospitalar Santa Casa de Porto Alegre, Porto Alegre, RS, Brazil.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Chem
authors.affiliation: Universidade Luterana do Brasil, Canoas, RS, Brazil.
authors.forename: Melissa
authors.initials: M
authors.lastname: Camassola
authors.affiliation: Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Nance Beyer
authors.initials: NB
authors.lastname: Nardi
authors.affiliation: Universidade Luterana do Brasil, Canoas, RS, Brazil.
authors.forename: Rafael Rodrigues
authors.initials: RR
authors.lastname: Dihl