title: Comparison of anti-inflammatory mechanisms of mango (Mangifera Indica L.) and pomegranate (Punica Granatum L.) in a preclinical model of colitis.
abstract: Tannin-rich fruits have been evaluated as alternative prevention strategies for colorectal cancer based on their anti-inflammatory properties. This study compared tannin-rich preparations from mango (rich in gallotannins) and pomegranate (rich in ellagitannins) in the dextran sodium sulfate-induced colitis model.
  
  
  
  In rats, mango and pomegranate beverages decreased intestinal inflammation and the levels of pro-inflammatory cytokines in mucosa and serum. The mango beverage suppressed the ratio of phosphorylated/total protein expression of the IGF-1R-AKT/mTOR axis and downregulated mRNA expression of Igf1, Insr, and pik3cv. Pomegranate decreased p70S6K and RPS6, as well as Rps6ka2, Map2k2, and Mapk1 mRNA. In silico modeling indicated a high binding of docked of gallic acid to the catalytic domain of IGF-1R, which may suppress the activity of the enzyme. Ellagic acid docked effectively into the catalytic domains of both IGF-1R and EGFR. In vitro assays with lipopolysaccharide-treated CCD-18Co cells using polyphenolic extracts from each beverage, as well as pure compounds, corroborated the predictions made in silico.
  
  
  
  Mango polyphenols inhibited the IGF-1R- AKT/mTOR axis, and pomegranate polyphenols downregulate the mTOR downstream pathway through reductions in ERK1/2. These results suggest that extracts rich in gallo- and ellagitannins act on different molecular targets in the protection against ulcerative colitis.
authors.affiliation: Department of Nutrition and Food Science, Texas A&M University, College Station, TX, USA.
authors.forename: Hyemee
authors.initials: H
authors.lastname: Kim
authors.affiliation: Interdisciplinary Program of Toxicology, Texas A&M University, College Station, TX, USA.
authors.forename: Nivedita
authors.initials: N
authors.lastname: Banerjee
authors.affiliation: Department of Veterinary Physiology and Pharmacology, Texas A&M University, College Station, TX, USA.
authors.forename: Ivan
authors.initials: I
authors.lastname: Ivanov
authors.affiliation: Department of Veterinary Pathobiology, Texas A&M University, College Station, TX, USA.
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Pfent
authors.affiliation: Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, USA.
authors.forename: Kalan R
authors.initials: KR
authors.lastname: Prudhomme
authors.affiliation: Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, USA.
authors.forename: William H
authors.initials: WH
authors.lastname: Bisson
authors.affiliation: Department of Nutrition and Food Science, Texas A&M University, College Station, TX, USA.|Center for Epigenetics & Disease Prevention, Texas A&M Health Science Center, Houston, TX, USA.
authors.forename: Roderick H
authors.initials: RH
authors.lastname: Dashwood
authors.affiliation: Department of Nutrition and Food Science, Texas A&M University, College Station, TX, USA.
authors.forename: Stephen T
authors.initials: ST
authors.lastname: Talcott
authors.affiliation: Department of Nutrition and Food Science, Texas A&M University, College Station, TX, USA. smtalcott@tamu.edu.|Department of Veterinary Physiology and Pharmacology, Texas A&M University, College Station, TX, USA. smtalcott@tamu.edu.
authors.forename: Susanne U
authors.initials: SU
authors.lastname: Mertens-Talcott
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000494:pharmacology*; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy*; D016264:Dextran Sulfate / Q000633:toxicity; D004195:Disease Models, Animal; D066246:ErbB Receptors / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D000067030:Fruit and Vegetable Juices; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D029561:Lythraceae / Q000737:chemistry*; D008297:Male; D031022:Mangifera / Q000737:chemistry*; D062105:Molecular Docking Simulation; D059808:Polyphenols / Q000494:pharmacology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D017526:Receptor, IGF Type 1 / Q000737:chemistry / Q000378:metabolism; D058570:TOR Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D059808:Polyphenols; D016264:Dextran Sulfate; C546845:mTOR protein, rat; C512478:EGFR protein, human; D066246:ErbB Receptors; D017526:Receptor, IGF Type 1; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: Colitis Mango mTOR pathway Pomegranate Rat