title: Modulatory effects of catechin hydrate against genotoxicity, oxidative stress, inflammation and apoptosis induced by benzo(a)pyrene in mice.
abstract: Benzo(a)pyrene [B(a)P], a polycyclic aromatic hydrocarbon (PAH) is a strong mutagen and potent carcinogen. The aim of the present study was to investigate the efficacy of catechin hydrate against B(a)P induced genotoxicity, oxidative stress, inflammation, apoptosis and to explore its underlying molecular mechanisms in the lungs of Swiss albino mice. Administration of B(a)P (125 mg/kg b. wt., p. o.) increased the activities of toxicity markers such as LPO, LDH and B(a)P metabolizing enzymes [NADPH-cytochrome P450 reductase (CYPOR) and microsomal epoxide hydrolase (mEH)] with subsequent decrease in the activities of tissue anti-oxidant armory (SOD, CAT, GPx, GR, GST, QR and GSH). It also caused DNA damage and activation of apoptotic and inflammatory pathway by upregulation of TNF-α, IL-6, NF-kB, COX-2, p53, bax, caspase-3 and down regulating Bcl-2. However, pre-treatment with catechin at a dose of 20 and 40 mg/kg significantly decreased LDH, LPO, B(a)P metabolizing enzymes and increased anti-oxidant armory as well as regulated apoptosis and inflammation in lungs. Histological results also supported the protective effects of catechin. The findings of the present studies suggested that catechin as an effective natural product attenuates B(a)P induced lung toxicity.
authors.affiliation: Section of Molecular Carcinogenesis and Chemoprevention Department of Medical Elementology and Toxicology, Faculty of Science Jamia Hamdard (Hamdard University), Hamdard Nagar New Delhi 110062, India.
authors.forename: Ayaz
authors.initials: A
authors.lastname: Shahid
authors.affiliation: Section of Molecular Carcinogenesis and Chemoprevention Department of Medical Elementology and Toxicology, Faculty of Science Jamia Hamdard (Hamdard University), Hamdard Nagar New Delhi 110062, India.
authors.forename: Rashid
authors.initials: R
authors.lastname: Ali
authors.affiliation: Section of Molecular Carcinogenesis and Chemoprevention Department of Medical Elementology and Toxicology, Faculty of Science Jamia Hamdard (Hamdard University), Hamdard Nagar New Delhi 110062, India.
authors.forename: Nemat
authors.initials: N
authors.lastname: Ali
authors.affiliation: Section of Molecular Carcinogenesis and Chemoprevention Department of Medical Elementology and Toxicology, Faculty of Science Jamia Hamdard (Hamdard University), Hamdard Nagar New Delhi 110062, India.
authors.forename: Syed Kazim
authors.initials: SK
authors.lastname: Hasan
authors.affiliation: Section of Molecular Carcinogenesis and Chemoprevention Department of Medical Elementology and Toxicology, Faculty of Science Jamia Hamdard (Hamdard University), Hamdard Nagar New Delhi 110062, India.
authors.forename: Preeti
authors.initials: P
authors.lastname: Bernwal
authors.affiliation: Section of Molecular Carcinogenesis and Chemoprevention Department of Medical Elementology and Toxicology, Faculty of Science Jamia Hamdard (Hamdard University), Hamdard Nagar New Delhi 110062, India.
authors.forename: Shekh Mohammad
authors.initials: SM
authors.lastname: Afzal
authors.affiliation: Section of Molecular Carcinogenesis and Chemoprevention Department of Medical Elementology and Toxicology, Faculty of Science Jamia Hamdard (Hamdard University), Hamdard Nagar New Delhi 110062, India.
authors.forename: Abul
authors.initials: A
authors.lastname: Vafa
authors.affiliation: Section of Molecular Carcinogenesis and Chemoprevention Department of Medical Elementology and Toxicology, Faculty of Science Jamia Hamdard (Hamdard University), Hamdard Nagar New Delhi 110062, India. Electronic address: sarwat786@rediffmail.com.
authors.forename: Sarwat
authors.initials: S
authors.lastname: Sultana
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D001564:Benzo(a)pyrene / Q000633:toxicity*; D002392:Catechin / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects* / Q000235:genetics; D007124:Immunoenzyme Techniques; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D008297:Male; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D016328:NF-kappa B; D001564:Benzo(a)pyrene; D002392:Catechin
keywords: Apoptosis; Benzo(a)pyrene; Catechin hydrate; Genotoxicity; Histopathology