title: Acyl hydrolases from trans-AT polyketide synthases target acetyl units on acyl carrier proteins.
abstract: Acyl hydrolase (AH) domains are a common feature of trans-AT PKSs. They have been hypothesised to perform a proofreading function by removing acyl chains from stalled sites. This study determines the substrate tolerance of the AH PedC for a range of acyl-ACPs. Clear preference towards short, linear acyl-ACPs is shown, with acetyl-ACP the best substrate. These results imply a more targeted housekeeping role for PedC: namely the removal of unwanted acetyl groups from ACP domains caused by erroneous transfer of acetyl-CoA, or possibly by decarboxylation of malonyl-ACP.
authors.affiliation: School of Chemistry, University of Nottingham, University Park, Nottingham, NG7 2RD, UK. neil.oldham@nottingham.ac.uk.
authors.forename: Matthew
authors.initials: M
authors.lastname: Jenner
authors.forename: Jose P
authors.initials: JP
authors.lastname: Afonso
authors.forename: Christoph
authors.initials: C
authors.lastname: Kohlhaas
authors.forename: Petra
authors.initials: P
authors.lastname: Karbaum
authors.forename: Sarah
authors.initials: S
authors.lastname: Frank
authors.forename: Jörn
authors.initials: J
authors.lastname: Piel
authors.forename: Neil J
authors.initials: NJ
authors.lastname: Oldham
mesh_terms: D000105:Acetyl Coenzyme A / Q000378:metabolism; D000213:Acyl Carrier Protein / Q000737:chemistry / Q000378:metabolism*; D000217:Acyltransferases / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D001419:Bacteria / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D003653:Decarboxylation; D006867:Hydrolases / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D008958:Models, Molecular; D048630:Polyketide Synthases / Q000737:chemistry / Q000378:metabolism*; D000072417:Protein Domains; D011714:Pyrans / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D000213:Acyl Carrier Protein; D011714:Pyrans; D000105:Acetyl Coenzyme A; D048630:Polyketide Synthases; C100127:pederin; D000217:Acyltransferases; D006867:Hydrolases