title: Efficient and Durable Vaccine against Intimin β of Diarrheagenic E. Coli Induced by Clay Nanoparticles.
abstract: Improved strategies are urgently required to control infections with enterohemorrhagic Escherichia coli and enteropathogenic E. coli, two dominant zoonotic enteric pathogens responsible for a wide spectrum of illnesses as well as deaths of human being, with tremendous financial cost worldwide. The present study investigates the capacity of two clay nanoparticles (NPs) with opposite surface charges, namely synthetic layered double hydroxide (LDH) and hectorite (HEC) NPs as adjuvants to promote strong immune responses against the infections. Here both LDH and HEC NPs are showed to be able to carry an appreciable amount of Intimin β (1.1 and 4.4 mg per mg clay nanomaterials, respectively) and significantly facilitate antigen uptake by antigen-presenting cells. Remarkably, these clay NPs induce strong antibody and cell-mediated immune responses, which are much higher than that by the potent adjuvant, QuilA. Furthermore, these strong immune responses are well maintained for at least four months in the mouse model, during which there are no changes in histopathology of the animal organs. Collectively these data demonstrate the suitability of LDH and HEC NPs as useful adjuvants in new-generation vaccine formulations to control various infectious diseases.
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St Lucia, QLD, 4072, Australia.
authors.forename: Weiyu
authors.initials: W
authors.lastname: Chen
authors.affiliation: Vaccine Delivery, Animal Science, Agri-Science Queensland Department of Agriculture, Fisheries and Forestry, St Lucia, QLD, 4067, Australia.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD, 4072, Australia.
authors.forename: Timothy
authors.initials: T
authors.lastname: Mahony
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St Lucia, QLD, 4072, Australia.
authors.forename: Wenyi
authors.initials: W
authors.lastname: Gu
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St Lucia, QLD, 4072, Australia.
authors.forename: Barbara
authors.initials: B
authors.lastname: Rolfe
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St Lucia, QLD, 4072, Australia.
authors.forename: Zhi Ping
authors.initials: ZP
authors.lastname: Xu
mesh_terms: D018829:Adhesins, Bacterial / Q000276:immunology*; D000538:Aluminum Silicates / Q000737:chemistry*; D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D000938:Antigen-Presenting Cells / Q000378:metabolism; D000942:Antigens, Bacterial / Q000378:metabolism; D000077215:Clay; D004705:Endocytosis; D058501:Enzyme-Linked Immunospot Assay; D004926:Escherichia coli / Q000276:immunology*; D029968:Escherichia coli Proteins / Q000276:immunology*; D022361:Escherichia coli Vaccines / Q000276:immunology*; D007111:Immunity, Cellular / Q000276:immunology; D007114:Immunization; D007371:Interferon-gamma / Q000378:metabolism; D008810:Mice, Inbred C57BL; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D018829:Adhesins, Bacterial; D000538:Aluminum Silicates; D000942:Antigens, Bacterial; D029968:Escherichia coli Proteins; D022361:Escherichia coli Vaccines; C073819:eaeA protein, E coli; D007371:Interferon-gamma; D000077215:Clay
keywords: clay nanoparticles; hectorite; intimin β; layered double hydroxides; vaccine adjuvants