title: Frying oils with high natural or added antioxidants content, which protect against postprandial oxidative stress, also protect against DNA oxidation damage.
abstract: OBJECTIVE
  Using sunflower oil as frying oil increases postprandial oxidative stress, which is considered the main endogenous source of DNA oxidative damage. We aimed to test whether the protective effect of virgin olive oil and oil models with added antioxidants against postprandial oxidative stress may also protect against DNA oxidative damage.
  
  
  METHODS
  Twenty obese people received four breakfasts following a randomized crossover design consisting of different oils [virgin olive oil (VOO), sunflower oil (SFO), and a mixed seed oil (SFO/canola oil) with added dimethylpolysiloxane (SOX) or natural antioxidants from olives (SOP)], which were subjected to 20 heating cycles.
  
  
  RESULTS
  We observed the postprandial increase in the mRNA levels of p53, OGG1, POLB, and GADD45b after the intake of the breakfast prepared with SFO and SOX, and an increase in the expression of MDM2, APEX1, and XPC after the intake of the breakfast prepared with SFO, whereas no significant changes at the postprandial state were observed after the intake of the other breakfasts (all p values <0.05). We observed lower 8-OHdG postprandial levels after the intake of the breakfast prepared with VOO and SOP than after the intake of the breakfast prepared with SFO and SOX (all p values <0.05).
  
  
  CONCLUSIONS
  Our results support the beneficial effect on DNA oxidation damage of virgin olive oil and the oil models with added antioxidants, as compared to the detrimental use of sunflower oil, which induces p53-dependent DNA repair pathway activation.
authors.affiliation: Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital/University of Córdoba, Av. Menendez Pidal s/n., 14004, Córdoba, Spain.|CIBER Fisiopatología de la obesidad y la nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Oriol A
authors.initials: OA
authors.lastname: Rangel-Zuñiga
authors.affiliation: Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital/University of Córdoba, Av. Menendez Pidal s/n., 14004, Córdoba, Spain.|CIBER Fisiopatología de la obesidad y la nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Haro
authors.affiliation: CIBER Fisiopatología de la obesidad y la nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.|Department of Biochemistry and Molecular Biology, Service of Clinical Analysis, General University Hospital-CDB, University of Valencia, Valencia, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Tormos
authors.affiliation: Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital/University of Córdoba, Av. Menendez Pidal s/n., 14004, Córdoba, Spain.|CIBER Fisiopatología de la obesidad y la nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: Perez-Martinez
authors.affiliation: Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital/University of Córdoba, Av. Menendez Pidal s/n., 14004, Córdoba, Spain.|CIBER Fisiopatología de la obesidad y la nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Delgado-Lista
authors.affiliation: Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital/University of Córdoba, Av. Menendez Pidal s/n., 14004, Córdoba, Spain.|CIBER Fisiopatología de la obesidad y la nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Marin
authors.affiliation: Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital/University of Córdoba, Av. Menendez Pidal s/n., 14004, Córdoba, Spain.|CIBER Fisiopatología de la obesidad y la nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Gracia M
authors.initials: GM
authors.lastname: Quintana-Navarro
authors.affiliation: CIBER Fisiopatología de la obesidad y la nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.|Department of Biochemistry and Molecular Biology, Service of Clinical Analysis, General University Hospital-CDB, University of Valencia, Valencia, Spain.
authors.forename: Concha
authors.initials: C
authors.lastname: Cerdá
authors.affiliation: CIBER Fisiopatología de la obesidad y la nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.|Department of Biochemistry and Molecular Biology, Service of Clinical Analysis, School of Medicine-INCLIVA, University Hospital Doctor PESET, University of Valencia, Valencia, Spain.
authors.forename: Guillermo T
authors.initials: GT
authors.lastname: Sáez
authors.affiliation: Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital/University of Córdoba, Av. Menendez Pidal s/n., 14004, Córdoba, Spain.|CIBER Fisiopatología de la obesidad y la nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Lopez-Segura
authors.affiliation: Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital/University of Córdoba, Av. Menendez Pidal s/n., 14004, Córdoba, Spain.|CIBER Fisiopatología de la obesidad y la nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Jose
authors.initials: J
authors.lastname: Lopez-Miranda
authors.affiliation: Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital/University of Córdoba, Av. Menendez Pidal s/n., 14004, Córdoba, Spain.|CIBER Fisiopatología de la obesidad y la nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Perez-Jimenez
authors.affiliation: Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital/University of Córdoba, Av. Menendez Pidal s/n., 14004, Córdoba, Spain. b92cagaa@uco.es.|CIBER Fisiopatología de la obesidad y la nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain. b92cagaa@uco.es.
authors.forename: Antonio
authors.initials: A
authors.lastname: Camargo
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D000943:Antigens, Differentiation / Q000235:genetics / Q000378:metabolism; D000975:Antioxidants / Q000008:administration & dosage* / Q000032:analysis; D062408:Breakfast; D018592:Cross-Over Studies; D004249:DNA Damage / Q000187:drug effects*; D045647:DNA Glycosylases / Q000235:genetics / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood / Q000652:urine; D004129:Dimethylpolysiloxanes / Q000008:administration & dosage / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009765:Obesity; D000069463:Olive Oil / Q000008:administration & dosage / Q000032:analysis; D018384:Oxidative Stress / Q000187:drug effects*; D010938:Plant Oils / Q000008:administration & dosage* / Q000032:analysis; D019518:Postprandial Period; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000074262:Rapeseed Oil / Q000008:administration & dosage / Q000032:analysis; D000074242:Sunflower Oil / Q000008:administration & dosage / Q000032:analysis; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D000097642:GADD45 Proteins
chemical_list: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000943:Antigens, Differentiation; D000975:Antioxidants; D045647:DNA Glycosylases; D003849:Deoxyguanosine; D004129:Dimethylpolysiloxanes; D000069463:Olive Oil; D010938:Plant Oils; D012333:RNA, Messenger; D000074262:Rapeseed Oil; D000074242:Sunflower Oil; D016159:Tumor Suppressor Protein p53; C086470:GADD45B protein, human; D000097642:GADD45 Proteins; C479058:oxoguanine glycosylase 1, human
keywords: DNA oxidation damage; Frying oils; Oxidative stress; Phenolic compounds; Virgin olive oil