title: Assessment of Periodontopathogens in Subgingival Biofilm of Banded and Bonded Molars in Early Phase of Fixed Orthodontic Treatment.
abstract: To assess the prevalence and occurrence of eleven periodontopathogens in subgingival biofilm of banded and bonded molars during the first period of fixed orthodontic treatment. Subjects were selected from patients referred to orthodontic treatment and were divided in two groups: group A comprised fifteen patients (14.4±2.45 years of age) who received orthodontic bands on first permanent molars and group B of ten patients (15.7±1.87 years of age) with directly bonded tubes on the labial surface of the same teeth. Subgingival sample collection was performed before bands and tubes application and 4-7 weeks after attachment placement. DNA-strip tehnique was used to assess the presence of eleven putative periodontopathogens at each time point. Fusobacterium nucleatum, Eikenella corrodens and Capnocytophaga spp. were found in a large number of samples, other periodontopathogens were present in a smaller rate. The 4-7 weeks after attachment placement a slight increase of putative species was observed in both groups. The presence of orthodontic tubes and bands influence the accumulation and composition of subgingival microbiota. Higher level of oral hygiene should be achieved before and during orthodontic treatment in order to prevent any side effects on periodontal tissues.
authors.affiliation: Department of Orthodontics, Faculty of Dental Medicine, University of Medicine and Pharmacy , Tirgu Mures , Roman.
authors.forename: Krisztina
authors.initials: K
authors.lastname: Mártha
authors.affiliation: Department of Microbiology, Faculty of General Medicine, University of Medicine and Pharmacy , Tirgu Mures , Roman.
authors.forename: Lilla
authors.initials: L
authors.lastname: Lőrinczi
authors.affiliation: Department of Pediatric Dentistry, Faculty of Dental Medicine, University of Medicine and Pharmacy , Tirgu Mures , Roman.
authors.forename: Cristina
authors.initials: C
authors.lastname: Bică
authors.affiliation: Department of Pediatric Dentistry, Faculty of Dental Medicine, University of Medicine and Pharmacy , Tirgu Mures , Roman.
authors.forename: Réka
authors.initials: R
authors.lastname: Gyergyay
authors.affiliation: Department of Preventive Dentistry, Faculty of Dental Medicine, University of Medicine and Pharmacy , Tirgu Mures , Roman.
authors.forename: Blanka
authors.initials: B
authors.lastname: Petcu
authors.affiliation: Department of Periodontology, Faculty of Dental Medicine, University of Medicine and Pharmacy , Tirgu Mures , Roman.
authors.forename: Luminița
authors.initials: L
authors.lastname: Lazăr
mesh_terms: D000293:Adolescent; D018441:Biofilms; D002206:Capnocytophaga / Q000302:isolation & purification / Q000502:physiology*; D002648:Child; D003773:Dental Plaque / Q000382:microbiology* / Q000628:therapy; D016124:Eikenella corrodens / Q000302:isolation & purification / Q000502:physiology*; D005260:Female; D016967:Fusobacterium nucleatum / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D008297:Male; D008963:Molar / Q000382:microbiology*; D009970:Orthodontics
keywords: DNA-strip technique; banded and bonded molars; periodontopathogens; subgingival biofilm