title: Study on JNK/AP-1 signaling pathway of airway mucus hypersecretion of severe pneumonia under RSV infection.
abstract: OBJECTIVE
  To investigate the JNK/AP-1 signaling pathway of airway mucus hypersecretion of severe pneumonia under respiratory virus (RSV) infection.
  
  
  METHODS
  Total of 56 severe pneumonia children under RSV infection were selected. Reverse transcription polymerase chain reaction (RT-PCR) was performed to measure the expression quantity of MUC5B mRNA and MUC5AC mRNA, and ELISA was used to measure the expression quantity of MUC5AC and MUC5B proteins. Following that, the children were divided into airway mucus hypersecretion group (n = 37) and non-hypersecretion group (n = 19). Western blotting was performed to detect the expression levels of JNK1/2, p-JNK1/2 and AP-1 proteins.
  
  
  RESULTS
  Expression of MUC5AC and MUC5B proteins, and MUC5AC mRNA and MUC5B mRNA in the airway mucus hypersecretion group were significantly higher than those in the non-hypersecretion group (p < 0.05). The expression levels of JNK1/2, p-JNK1/2 and AP-1 proteins in airway mucus hypersecretion group were higher than those in the non-hypersecretion group (p < 0.05).
  
  
  CONCLUSIONS
  MUC5AC and MUC5B can be used as marker molecules of airway mucus hypersecretion. Airway mucus hypersecretion of severe pneumonia induced by RSV might be related to the activation of JNK/AP-1 signaling pathway.
authors.affiliation: Department of Pediatrics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China. lilylxm@163.com.
authors.forename: X-M
authors.initials: XM
authors.lastname: Li
authors.forename: S-Z
authors.initials: SZ
authors.lastname: Sun
authors.forename: F-L
authors.initials: FL
authors.lastname: Wu
authors.forename: T
authors.initials: T
authors.lastname: Shi
authors.forename: H-J
authors.initials: HJ
authors.lastname: Fan
authors.forename: D-Z
authors.initials: DZ
authors.lastname: Li
mesh_terms: D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D020935:MAP Kinase Signaling System / Q000235:genetics; D008297:Male; D055271:Mucin 5AC / Q000235:genetics / Q000378:metabolism; D055257:Mucin-5B / Q000235:genetics / Q000378:metabolism; D009093:Mucus / Q000378:metabolism*; D011014:Pneumonia / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D012137:Respiratory System / Q000378:metabolism* / Q000821:virology; D010253:Respirovirus Infections / Q000150:complications / Q000235:genetics* / Q000378:metabolism; D012720:Severity of Illness Index; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism
chemical_list: C524678:MUC5AC protein, human; C524578:MUC5B protein, human; D055271:Mucin 5AC; D055257:Mucin-5B; D018808:Transcription Factor AP-1