title: ISOLATION AND GENOTYPING OF CLOSTRIDIUM PERFRINGENS FROM FREE-LIVING SOUTH AMERICAN COATI (NASUA NASUA).
abstract: The importance of Clostridium perfringens for most wild animal species remains unclear. This study aimed to isolate and genotype C. perfringens in stool samples from free-living South American coati (Nasua nasua) in Brazil. Forty-six free-living N. nasua were trapped and stool samples were collected. Two different protocols for C. perfringens isolation were tested: direct plating onto selective agar and pre-enrichment in broth followed by plating in selective agar. Clostridium perfringens type A was isolated from 15 (32.6%) animals by direct plating and 36 (78.3%) animals by broth PE, and the rate of isolation was significantly different between these two methods (P < 0.01). Twelve of the 36 (33.3%) isolated strains by the PE protocol were positive for the β-2 toxin-encoding gene (cpb2) whereas the enterotoxin-encoding gene (cpe) and necrotic enteritis like-B toxin gene (netb) were not found. These results suggest that C. perfringens is commonly part of the microbiota of free-living coatis. Additionally, the use of a PE protocol appears to be essential for studies on C. perfringens in this species.
authors.forename: Rodrigo O S
authors.initials: RO
authors.lastname: Silva
authors.forename: Lara R
authors.initials: LR
authors.lastname: Almeida
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Oliveira Junior
authors.forename: Paula C S
authors.initials: PC
authors.lastname: Lima
authors.forename: Danielle F M
authors.initials: DF
authors.lastname: Soares
authors.forename: Pedro L L
authors.initials: PL
authors.lastname: Pereira
authors.forename: Israel J
authors.initials: IJ
authors.lastname: Silva
authors.forename: Francisco C F
authors.initials: FC
authors.lastname: Lobato
mesh_terms: D000818:Animals; D000835:Animals, Wild; D003015:Clostridium Infections / Q000382:microbiology / Q000662:veterinary*; D003016:Clostridium perfringens / Q000235:genetics* / Q000302:isolation & purification*; D005260:Female; D005838:Genotype; D008297:Male; D045988:Procyonidae
keywords: Nasua nasua; coatis; diarrhea; enteritis; procyonid; β-2