title: Studying Bacterial Multispecies Biofilms: Where to Start?
abstract: The high prevalence and significance of multispecies biofilms have now been demonstrated in various bacterial habitats with medical, industrial, and ecological relevance. It is highly evident that several species of bacteria coexist and interact in biofilms, which highlights the need for evaluating the approaches used to study these complex communities. This review focuses on the establishment of multispecies biofilms in vitro, interspecies interactions in microhabitats, and how to select communities for evaluation. Studies have used different experimental approaches; here we evaluate the benefits and drawbacks of varying the degree of complexity. This review aims to facilitate multispecies biofilm research in order to expand the current limited knowledge on interspecies interactions.
authors.affiliation: Section of Microbiology, Universitetsparken 15, 2100 Copenhagen Ø, Department of Biology, Faculty of Science, University of Copenhagen, Denmark.
authors.forename: Henriette L
authors.initials: HL
authors.lastname: Røder
authors.affiliation: Section of Microbiology, Universitetsparken 15, 2100 Copenhagen Ø, Department of Biology, Faculty of Science, University of Copenhagen, Denmark.
authors.forename: Søren J
authors.initials: SJ
authors.lastname: Sørensen
authors.affiliation: Section of Microbiology, Universitetsparken 15, 2100 Copenhagen Ø, Department of Biology, Faculty of Science, University of Copenhagen, Denmark. Electronic address: burmolle@bio.ku.dk.
authors.forename: Mette
authors.initials: M
authors.lastname: Burmølle
mesh_terms: D001419:Bacteria / Q000254:growth & development / Q000378:metabolism*; D018407:Bacterial Physiological Phenomena; D018441:Biofilms / Q000145:classification*; D004463:Ecology; D004777:Environment; D059013:Microbial Consortia / Q000502:physiology; D056265:Microbial Interactions / Q000502:physiology
keywords: bacterial interactions; biofilm; communities; in vitro; multispecies