title: Aqueous two-phase system purification for superoxide dismutase induced by menadione from Phanerochaete chrysosporium.
abstract: In the present work, the partitioning behavior of menadione-induced superoxide dismutase (SOD; EC 1.15.1.1), an antioxidant enzyme that has various applications in the medical and cosmetic industries, from the white rot fungus Phanerochaete chrysosporium has been characterized on different types of aqueous two-phase systems (ATPSs) (poly(ethylene glycol)/polypropylene glycol (PEG/PPG)-dextran, PEG-salt and PPG-salt). PEG-salt combinations were found most optimal systems for the purification of SOD. The best partition conditions were found using the PEG-3350 24% and K2HPO45% (w/w) with pH 7.0 at 25 °C. The partition coefficient of total SOD activity and total protein concentration observed in this system were 0.17 and 6.65, respectively, with the recovery percentage as 78.90% in the bottom phase and 13.17% in the top phase. The highest purification fold for SOD from P. chrysosporium was found as 6.04 in the bottom phase of PEG 3350%24 - K2HPO4%5 (w/w) system with pH 7.0. SOD purified from P. chrysosporium was determined to be a homodimer in its native state with a molecular weight of 60  ± 4 kDa. Consequently, simple and only one step PEG-salt ATPS system was developed for SOD purification from P. chrysosporium.
authors.affiliation: a Department of Chemistry, Faculty of Science , University of Dokuz Eylul , Buca, Izmir , Turkey.
authors.forename: Berna
authors.initials: B
authors.lastname: Kavakcıoğlu
authors.affiliation: a Department of Chemistry, Faculty of Science , University of Dokuz Eylul , Buca, Izmir , Turkey.
authors.forename: Burcu
authors.initials: B
authors.lastname: Tongul
authors.affiliation: a Department of Chemistry, Faculty of Science , University of Dokuz Eylul , Buca, Izmir , Turkey.
authors.forename: Leman
authors.initials: L
authors.lastname: Tarhan
mesh_terms: D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification*; D020075:Phanerochaete / Q000201:enzymology*; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011409:Propylene Glycols / Q000737:chemistry; D013482:Superoxide Dismutase / Q000737:chemistry / Q000302:isolation & purification*; D024483:Vitamin K 3 / Q000494:pharmacology*
chemical_list: D005656:Fungal Proteins; D011108:Polymers; D011409:Propylene Glycols; C012504:polypropylene glycol; D011092:Polyethylene Glycols; D024483:Vitamin K 3; D013482:Superoxide Dismutase; C000595209:polyethylene glycol 1000
keywords: Aqueous two-phase systems; partitioning; polymer/salt system; purification; superoxide dismutase