title: Automated synthesis of [(18)F](2S,4R)-4-fluoroglutamine on a GE TRACERlab™ FX-N Pro module.
abstract: Glutamine (Gln) and its analogues may serve as imaging agents for tumor diagnosis using positron emission tomography (PET), especially for tumors with negative [(18)F]FDG scan. We report the first automated synthesis of [(18)F](2S,4R)-4-fluoroglutamine ([(18)F]FGln) on a GE TRACERlab™ FX-N Pro module. [(18)F]FGln was obtained in 80±3min with a radiochemical yield of 21±3% (n=5, uncorrected). The radiochemical purity was >98%, and optical purity 90±5%. The synthesis is highly reproducible with good chemical purity, radiochemical yield, and is suitable for translation to cGMP production.
authors.affiliation: Imaging Probe Development Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Rockville, MD, USA.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Imaging Probe Development Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Rockville, MD, USA.
authors.forename: Falguni
authors.initials: F
authors.lastname: Basuli
authors.affiliation: Imaging Probe Development Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Rockville, MD, USA.
authors.forename: Zhen-Dan
authors.initials: ZD
authors.lastname: Shi
authors.affiliation: Imaging Probe Development Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Rockville, MD, USA.
authors.forename: Biying
authors.initials: B
authors.lastname: Xu
authors.affiliation: Imaging Probe Development Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Rockville, MD, USA.
authors.forename: Burchelle
authors.initials: B
authors.lastname: Blackman
authors.affiliation: Molecular Imaging Program, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Peter L
authors.initials: PL
authors.lastname: Choyke
authors.affiliation: Imaging Probe Development Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Rockville, MD, USA. Electronic address: rolf.swenson@nih.gov.
authors.forename: Rolf E
authors.initials: RE
authors.lastname: Swenson
mesh_terms: D001331:Automation / Q000295:instrumentation / Q000379:methods; D060326:Chemistry Techniques, Synthetic / Q000295:instrumentation / Q000379:methods; D004355:Drug Stability; D005462:Fluorine Radioisotopes; D005973:Glutamine / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D006801:Humans; D009369:Neoplasms / Q000000981:diagnostic imaging; D049268:Positron-Emission Tomography / Q000379:methods; D019275:Radiopharmaceuticals / Q000138:chemical synthesis*; D013679:Technology, Radiologic / Q000295:instrumentation / Q000379:methods
chemical_list: C569565:4-fluoroglutamine; D005462:Fluorine Radioisotopes; D019275:Radiopharmaceuticals; D005973:Glutamine
keywords: Automation; F-18; Fluoroglutamine; PET imaging; Tumor