title: Molecular basis of 5-hydroxytryptophan synthesis in Saccharomyces cerevisiae.
abstract: We report for the first time that 5-hydroxytryptophan can be synthesized in Saccharomyces cerevisiae by heterologously expressing prokaryotic phenylalanine 4-hydroxylase or eukaryotic tryptophan 3/5-hydroxylase, together with enhanced synthesis of MH4 or BH4 cofactors. The innate DFR1 gene in the folate synthesis pathway was found to play pivotal roles in 5-hydroxytryptophan synthesis.
authors.affiliation: Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. xueyang@vt.edu and Department of Pharmacology & Toxicology, University of Arizona, Tucson, AZ 85721, USA.
authors.forename: Jiantao
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. xueyang@vt.edu.
authors.forename: Chaochen
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. xueyang@vt.edu.
authors.forename: Jiayuan
authors.initials: J
authors.lastname: Sheng
authors.affiliation: Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. xueyang@vt.edu.
authors.forename: Xueyang
authors.initials: X
authors.lastname: Feng
mesh_terms: D006916:5-Hydroxytryptophan / Q000096:biosynthesis*; D001708:Biopterins / Q000031:analogs & derivatives / Q000378:metabolism; D005492:Folic Acid / Q000096:biosynthesis; D015966:Gene Expression Regulation, Fungal; D010651:Phenylalanine Hydroxylase / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D014365:Tryptophan Hydroxylase / Q000235:genetics / Q000378:metabolism
chemical_list: D001708:Biopterins; D005492:Folic Acid; D006916:5-Hydroxytryptophan; D010651:Phenylalanine Hydroxylase; D014365:Tryptophan Hydroxylase; C003402:sapropterin