title: Detection of variant infectious bronchitis viruses in Sri Lanka (2012-2015).
abstract: Poultry production is an important sector of agriculture in Sri Lanka; however, there is a lack of information regarding circulation of infectious bronchitis virus (IBV). RNA was extracted from chicken tissues, subjected to IBV S1 RT-PCR, and sequenced. Overall, 19 out of 34 (55.88 %) samples were IBV positive and contained the genotype 793B (n = 13; 68.42 %), D274 (n = 4; 21.05 %) or Massachusetts (n = 2; 10.53 %). All three genotypes contained at least one strain with less than 99 % nucleotide sequence identity to the corresponding vaccine strains. This report identified co-circulation of IBV strains 793B, Massachusetts and D274, in Sri Lanka that are divergent from the respective vaccine strains.
authors.affiliation: Institute of Infection and Global Health, University of Liverpool, Leahurst Campus, Neston, Cheshire, CH64 7TE, UK.
authors.forename: Christopher
authors.initials: C
authors.lastname: Ball
authors.affiliation: Institute of Infection and Global Health, University of Liverpool, Leahurst Campus, Neston, Cheshire, CH64 7TE, UK.
authors.forename: Anne
authors.initials: A
authors.lastname: Forrester
authors.affiliation: Institute of Infection and Global Health, University of Liverpool, Leahurst Campus, Neston, Cheshire, CH64 7TE, UK. gana@liv.ac.uk.
authors.forename: Kannan
authors.initials: K
authors.lastname: Ganapathy
mesh_terms: D000818:Animals; D002645:Chickens / Q000821:virology; D018352:Coronavirus Infections / Q000662:veterinary* / Q000821:virology; D014644:Genetic Variation; D005838:Genotype; D001351:Infectious bronchitis virus / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification*; D010802:Phylogeny; D011201:Poultry Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D013188:Sri Lanka; D014765:Viral Vaccines / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014765:Viral Vaccines