title: Evaluation of autologous bone marrow-derived mesenchymal stem cells on renal regeneration after experimentally induced acute kidney injury in dogs.
abstract: OBJECTIVE
  To evaluate the usefulness of autologous bone marrow-derived mesenchymal stem cell (BM-MSC) therapy for the treatment of dogs with experimentally induced acute kidney injury.
  
  
  METHODS
  6 healthy dogs.
  
  
  METHODS
  After induction of kidney injury (day 0) with cisplatin (5 mg/kg, IV), dogs immediately received saline (0.9% NaCl) solution (10 mL; n = 3) or BM-MSCs (1 × 10(6) cells/kg in 10 mL of saline solution; 3) IV. A CBC, serum biochemical analysis, and urinalysis were performed for each dog before administration of cisplatin and on days 1 through 4. Glomerular filtration rate was determined for all dogs on days -7 and 2; BM-MSC tracking by MRI was performed on BM-MSC-treated dogs on days -14 and 4. After sample collection and BM-MSC tracking on day 4, all dogs were euthanized; kidney tissue samples underwent histologic evaluation, immunohistochemical analysis, and cytokine profiling via reverse transcriptase PCR assays.
  
  
  RESULTS
  Kidney tissue from both groups had mononuclear inflammatory cell infiltration, tubular necrosis, dilated tubules, and glomerular damage. However, there was less fibrotic change and increased proliferation of renal tubular epithelial cells in the BM-MSC-treated dogs, compared with findings for the control dogs. Expressions of tumor necrosis factor-α and transforming growth factor-β were lower in the BM-MSC-treated group, compared with findings for the control group. Laboratory data revealed no improvement in the renal function in BM-MSC-treated dogs.
  
  
  CONCLUSIONS
  Results of this study suggested that autologous BM-MSCs may accelerate renal regeneration after experimentally induced acute kidney injury in dogs.
authors.forename: Chae-Young
authors.initials: CY
authors.lastname: Lim
authors.forename: Jae-Ik
authors.initials: JI
authors.lastname: Han
authors.forename: Seung-Gon
authors.initials: SG
authors.lastname: Kim
authors.forename: Chang-Min
authors.initials: CM
authors.lastname: Lee
authors.forename: Hee-Myung
authors.initials: HM
authors.lastname: Park
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced* / Q000628:therapy; D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D002945:Cisplatin / Q000633:toxicity; D004285:Dogs; D005919:Glomerular Filtration Rate; D007668:Kidney / Q000503:physiopathology; D045164:Mesenchymal Stem Cell Transplantation / Q000662:veterinary*; D059630:Mesenchymal Stem Cells; D012038:Regeneration / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha
chemical_list: D014409:Tumor Necrosis Factor-alpha; D002945:Cisplatin