title: A fast protocol for purification of translating mRNAs.
abstract: We present a protocol that enables easy extraction of translating mRNAs by purification of tagged ribosomes and associated mRNAs. By studying three different examples of stress influenced genes, we demonstrate the effectiveness and compare our rapid method to an older protocol (Halbeisen et al., 2009).
authors.affiliation: Institute of Biochemistry and Molecular Medicine (IBMM), Bühlstrasse 28, 3012 Bern, Switzerland. Electronic address: daniela.ross@ibmm.unibe.ch.
authors.forename: Daniela
authors.initials: D
authors.lastname: Ross
authors.affiliation: Institute of Biochemistry and Molecular Medicine (IBMM), Bühlstrasse 28, 3012 Bern, Switzerland. Electronic address: michael.altmann@ibmm.unibe.ch.
authors.forename: Michael
authors.initials: M
authors.lastname: Altmann
mesh_terms: D002458:Cell Fractionation / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D014176:Protein Biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D012270:Ribosomes / Q000737:chemistry / Q000235:genetics*
chemical_list: D012333:RNA, Messenger
keywords: GCN4; GRE1; HSF1; Ribosome purification; Translation under stress conditions