title: Neuroprotective effect of ischemic preconditioning via modulating the expression of adropin and oxidative markers against transient cerebral ischemia in diabetic rats.
abstract: Ischemic preconditioning (IPreC) can render the brain more tolerant to a subsequent potential lethal ischemic injury. Hyperglycemia has been shown to increase the size of ischemic stroke and worsen the clinical outcome following a stroke, thus exacerbating oxidative stress. Adropin has a significant association with cardiovascular disease, especially with diabetes. In this study, we aimed to evaluate the role of the IPreC due to modulating the expression of adropin and oxidative damage markers against stroke by induced transient middle cerebral artery occlusion (MCAo) in streptozotocin (STZ)-induced diabetic rats.
  
  
  
  72 male Spraque Dawley rats were allocated to 8 groups. In order to evaluate alterations of anti/oxidative status and adropin level, we induced transient MCAo seven days after STZ-induced diabetes. Also we performed IPreC 72h before transient MCAo to assess whether IPreC could have a neuroprotective effect against ischemia-reperfusion injury.
  
  
  
  The general characteristics of STZ-treated rats (STZ) included reduced body weight and elevated blood glucose levels compared to non-diabetic ones. Ischemic preconditioning before cerebral ischemia significantly reduced infarction size compared with the other groups [IPreC+MCAo (27±11mm(3)) vs. MCAo (109±17mm(3)) p<0.001; STZ+IPreC+MCAo (38±10mm(3)) vs. STZ+MCAo (165±45mm(3)) p<0.001, respectively]. The mean total antioxidant status level in IPreC groups was higher than other groups (p≤0.05). Moreover, IPreC considerably decreased mean adropin levels compared with other groups (p≤0.05).
  
  
  
  The study results supported the neuroprotective effects of ischemic preconditioning in MCA infarcts correlated with the level of oxidative damage markers and adropin.
authors.affiliation: Bor State Hospital, Neurology Clinic, Istasyon Street, 51700 Bor, Nigde, Turkey. Electronic address: dr.ozgealtintas@gmail.com.
authors.forename: O
authors.initials: O
authors.lastname: Altintas
authors.affiliation: BezmiAlem Vakif University, Vocational School of Health Services, Medical Laboratory Techniques, Adnan Menderes Bulvarı, 34093 Fatih, Istanbul, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Kumas
authors.affiliation: Fatih University, Faculty of Engineering, Department of Genetics and Bioengineering, Buyukcekmece Campus, 34500 Buyukcekmece, Istanbul, Turkey.
authors.forename: M O
authors.initials: MO
authors.lastname: Altintas
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001798:Blood Proteins / Q000235:genetics / Q000378:metabolism*; D001921:Brain / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000378:metabolism*; D005786:Gene Expression Regulation; D020244:Infarction, Middle Cerebral Artery / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D019194:Ischemic Preconditioning; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D017207:Rats, Sprague-Dawley
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; C586182:ENHO protein, rat; D010455:Peptides
keywords: Adropin; Neuroprotection; Oxidative stress; Preconditioning; Transient ischemia