title: Deoxynivalenol induces apoptosis in PC12 cells via the mitochondrial pathway.
abstract: Deoxynivalenol (DON) has broad toxicity in animals and humans. In this study the impact of DON treatment on apoptotic pathways in PC12 cells was determined. The effects of DON were evaluated on (i) typical indicators of apoptosis, including cellular morphology, cell activity, lactate dehydrogenase (LDH) release, and apoptosis ratio in PC12 cells, and on (ii) the expression of key genes and proteins related to apoptosis, including Bcl-2, Bax, Bid, cytochrome C (Cyt C), apoptosis inducing factor (AIF), cleaved-Caspase9, and cleaved-Caspase3. DON treatment inhibited proliferation of PC12 cells, induced significant morphological changes and apoptosis, promoted the release of Cyt C and AIF from the mitochondria, and increased the activities of cleaved-Caspase9 and cleaved-Caspase3. Bcl-2 expression decreased with increasing DON concentrations, in contrast to Bax and Bid, which were increased with increasing DON concentration. These data demonstrate that DON induces apoptosis in PC12 cells through the mitochondrial apoptosis pathway.
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, China. Electronic address: wangxichun@ahau.edu.cn.
authors.forename: Xichun
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, China. Electronic address: xwdd136@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, China. Electronic address: 1464981421@qq.com.
authors.forename: Mengxue
authors.initials: M
authors.lastname: Fan
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, China. Electronic address: 649449698@qq.com.
authors.forename: Tingting
authors.initials: T
authors.lastname: Meng
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, China. Electronic address: 912012949@qq.com.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, China. Electronic address: 597913559@qq.com.
authors.forename: Yunjing
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, China. Electronic address: 1473007481@qq.com.
authors.forename: Dianfeng
authors.initials: D
authors.lastname: Zhu
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, China. Electronic address: 1299791970@qq.com.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Hu
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, China. Electronic address: 798500733@qq.com.
authors.forename: Jiajie
authors.initials: J
authors.lastname: Gong
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, China. Electronic address: luyifsb@126.com.
authors.forename: Shibin
authors.initials: S
authors.lastname: Feng
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, China. Electronic address: wjj@ahau.edu.cn.
authors.forename: Jinjie
authors.initials: J
authors.lastname: Wu
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, China. Electronic address: lydhy2014@ahau.edu.cn.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D045304:Cytochromes c / Q000378:metabolism; D015386:Hazardous Substances / Q000633:toxicity*; D008928:Mitochondria; D016716:PC12 Cells; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D014255:Trichothecenes / Q000633:toxicity*
chemical_list: D015386:Hazardous Substances; D019253:Proto-Oncogene Proteins c-bcl-2; D014255:Trichothecenes; D045304:Cytochromes c; D053148:Caspase 3; C007262:deoxynivalenol
keywords: Apoptosis; Cytotoxicity; Deoxynivalenol; Neurotoxicity; PC12 cell