title: Detection of Circulating Tumor DNA in Human Blood via DNA-Mediated Surface-Enhanced Raman Spectroscopy of Single-Walled Carbon Nanotubes.
abstract: The levels of circulating tumor DNA (ctDNA) in the peripheral blood have been associated with tumor burden and malignant progression. However, ultrasensitive detection of ctDNA in blood remains to be explored. Herein, we have developed a new approach, employing DNA-mediated surface-enhanced Raman scattering (SERS) of single-walled carbon nanotubes (SWNTs), that allows ultrasensitive detection of a broad range of ctDNAs in human blood. Combined with the efficient ctDNA recognition capacity of our designed triple-helix molecular switch and RNase HII enzyme-assisted amplification, the T-rich DNA-mediated SERS enhancement of SWNTs could read out a content of KRAS G12DM as low as 0.3 fM, with a detection of 5.0 μL of sample volume, which has potential for point-of-care testing in clinical analysis.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, China.
authors.forename: Qifeng
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zheng
authors.affiliation: School of Chemistry and Biological Engineering, Changsha University of Science and Technology , Changsha 410004, China.
authors.forename: Zhihe
authors.initials: Z
authors.lastname: Qing
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, China.
authors.forename: Mengjie
authors.initials: M
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Yang
authors.affiliation: School of Chemistry and Biological Engineering, Changsha University of Science and Technology , Changsha 410004, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, China.
authors.forename: Le
authors.initials: L
authors.lastname: Ying
authors.affiliation: School of Chemistry and Biological Engineering, Changsha University of Science and Technology , Changsha 410004, China.
authors.forename: Ronghua
authors.initials: R
authors.lastname: Yang
mesh_terms: D000074141:Circulating Tumor DNA / Q000097:blood*; D004273:DNA, Neoplasm / Q000097:blood*; D006801:Humans; D037742:Nanotubes, Carbon / Q000737:chemistry*; D013059:Spectrum Analysis, Raman; D013499:Surface Properties
chemical_list: D000074141:Circulating Tumor DNA; D004273:DNA, Neoplasm; D037742:Nanotubes, Carbon