title: A high-throughput multiplex genetic detection system for Helicobacter pylori identification, virulence and resistance analysis.
abstract: We established a high-throughput multiplex genetic detection system (HMGS) for identification of Helicobacter pylori with concomitant analysis of virulence and drug resistance.
  
  
  
  Confirmed 132 H. pylori cultures from gastric biopsies were screened by 20-gene site-HMGS, sequencing and E-test.
  
  
  
  HMGS was highly sensitive and specific for H. pylori identification. Concordance rate between HMGS and sequencing averaged 94.5% (virulence genes) and 97.3% (resistance genes). Observed resistance rates to four mainstream antibiotics were high, except for amoxicillin. Significant association between virulence genotype and risks for specific gastrointestinal diseases was found for five genes. Metronidazole resistance in peptic ulcer patients was significantly higher.
  
  
  
  HMGS is an effective method for H. pylori identification and analysis of virulence and drug resistance.
authors.affiliation: Department of Laboratory Medicine, Huadong Hospital Affiliated to Fudan University, No. 221 Yanan West Road, Shanghai, 200040, China.
authors.forename: Binjie
authors.initials: B
authors.lastname: Hu
authors.affiliation: Department of Laboratory Medicine, Huadong Hospital Affiliated to Fudan University, No. 221 Yanan West Road, Shanghai, 200040, China.
authors.forename: Fuju
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Department of Laboratory Medicine, Huadong Hospital Affiliated to Fudan University, No. 221 Yanan West Road, Shanghai, 200040, China.
authors.forename: Shiwen
authors.initials: S
authors.lastname: Wang
authors.affiliation: Division of Pulmonary & Critical Care Medicine, Department of Internal Medicine, University of Michigan Health System & Research Service, VA Ann Arbor Health Systems, Ann Arbor, MI, USA.
authors.forename: Michal A
authors.initials: MA
authors.lastname: Olszewski
authors.affiliation: Department of Gastroenterology, Tongji Hospital Affiliated to Shanghai Tongji University, Shanghai, China.
authors.forename: Haipeng
authors.initials: H
authors.lastname: Bian
authors.affiliation: Ningbo HEALTH Gene Technologies Co., Ltd., Ningbo, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Ningbo HEALTH Gene Technologies Co., Ltd., Ningbo, China.
authors.forename: Mimi
authors.initials: M
authors.lastname: Kong
authors.affiliation: Shanghai ABSciex Analytical Instrument Trading Co., Ltd., Shanghai, China.
authors.forename: Lingli
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Laboratory Medicine, Huadong Hospital Affiliated to Fudan University, No. 221 Yanan West Road, Shanghai, 200040, China.
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Miao
authors.affiliation: Department of Laboratory Medicine, Huadong Hospital Affiliated to Fudan University, No. 221 Yanan West Road, Shanghai, 200040, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Department of Gastroenterology, Tongji Hospital Affiliated to Shanghai Tongji University, Shanghai, China.
authors.forename: Changqing
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Laboratory Medicine, Huadong Hospital Affiliated to Fudan University, No. 221 Yanan West Road, Shanghai, 200040, China.|Key Laboratory of Clinical Geriatric Medicine, Shanghai, China.|Research Center on Aging & Medicine, Fudan University, Shanghai, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Laboratory Medicine, Huadong Hospital Affiliated to Fudan University, No. 221 Yanan West Road, Shanghai, 200040, China.|Key Laboratory of Clinical Geriatric Medicine, Shanghai, China.|Research Center on Aging & Medicine, Fudan University, Shanghai, China.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D001706:Biopsy / Q000379:methods; D002681:China; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial / Q000302:isolation & purification; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005260:Female; D005767:Gastrointestinal Diseases / Q000235:genetics / Q000382:microbiology; D041981:Gastrointestinal Tract / Q000382:microbiology; D005798:Genes, Bacterial / Q000235:genetics; D005838:Genotype; D016481:Helicobacter Infections / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D016480:Helicobacter pylori / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008297:Male; D008795:Metronidazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction; D010437:Peptic Ulcer / Q000188:drug therapy / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics*; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D037521:Virulence Factors; D008795:Metronidazole
keywords: Helicobacter pylori; drug resistance; high-throughput multiplex genetic detection system; identification; virulence