title: Butylphthalide Suppresses Neuronal Cells Apoptosis and Inhibits JNK-Caspase3 Signaling Pathway After Brain Ischemia /Reperfusion in Rats.
abstract: Although Butylphthalide (BP) has protective effects that reduce ischemia-induced brain damage and neuronal cell death, little is known about the precise mechanisms occurring during cerebral ischemia/reperfusion (I/R). Therefore, the aim of this study was to investigate the neuroprotective mechanisms of BP against ischemic brain injury induced by cerebral I/R through inhibition of the c-Jun N-terminal kinase (JNK)-Caspase3 signaling pathway. BP in distilled non-genetically modified Soybean oil was administered intragastrically three times a day at a dosage of 15 mg/(kg day) beginning at 20 min after I/R in Sprague-Dawley rats. Immunohistochemical staining and Western blotting were performed to examine the expression of related proteins, and TUNEL-staining was used to detect the percentage of neuronal apoptosis in the hippocampal CA1 region. The results showed that BP could significantly protect neurons against cerebral I/R-induced damage. Furthermore, the expression of p-JNK, p-Bcl2, p-c-Jun, FasL, and cleaved-caspase3 was also decreased in the rats treated with BP. In summary, our results imply that BP could remarkably improve the survival of CA1 pyramidal neurons in I/R-induced brain injury and inhibit the JNK-Caspase3 signaling pathway.
authors.affiliation: Jiangsu Key Laboratory of Brain Disease Bioinformation, Xuzhou Medical College, Xuzhou, Jiangsu, 221004, People's Republic of China.|School of Basic Education Sciences, Xuzhou Medical College, Xuzhou, Jiangsu, 221004, People's Republic of China.
authors.forename: Xiang-Ru
authors.initials: XR
authors.lastname: Wen
authors.affiliation: Jiangsu Key Laboratory of Brain Disease Bioinformation, Xuzhou Medical College, Xuzhou, Jiangsu, 221004, People's Republic of China.|Department of Anatomy, Xuzhou Medical College, Xuzhou, Jiangsu, 221004, People's Republic of China.
authors.forename: Man
authors.initials: M
authors.lastname: Tang
authors.affiliation: Jiangsu Key Laboratory of Brain Disease Bioinformation, Xuzhou Medical College, Xuzhou, Jiangsu, 221004, People's Republic of China.|Department of Genetics, Research Center for Neurobiology, Xuzhou Medical College, 209 Tongshan Road, Xuzhou, Jiangsu, 221004, People's Republic of China.
authors.forename: Da-Shi
authors.initials: DS
authors.lastname: Qi
authors.affiliation: School of Public Health, Xuzhou Medical College, Xuzhou, Jiangsu, 221004, People's Republic of China.
authors.forename: Xiao-Jing
authors.initials: XJ
authors.lastname: Huang
authors.affiliation: Jiangsu Key Laboratory of Brain Disease Bioinformation, Xuzhou Medical College, Xuzhou, Jiangsu, 221004, People's Republic of China.
authors.forename: Hong-Zhi
authors.initials: HZ
authors.lastname: Liu
authors.affiliation: Jiangsu Key Laboratory of Brain Disease Bioinformation, Xuzhou Medical College, Xuzhou, Jiangsu, 221004, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Jiangsu Key Laboratory of Brain Disease Bioinformation, Xuzhou Medical College, Xuzhou, Jiangsu, 221004, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.affiliation: School of Public Health, Xuzhou Medical College, Xuzhou, Jiangsu, 221004, People's Republic of China.
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Wang
authors.affiliation: School of Public Health, Xuzhou Medical College, Xuzhou, Jiangsu, 221004, People's Republic of China.
authors.forename: Xun-Bao
authors.initials: XB
authors.lastname: Zhang
authors.affiliation: Department of Nuclear Medicine, The General Hospital of Xuzhou Coal Mining Group, Xuzhou, Jiangsu, 221006, People's Republic of China.
authors.forename: Ji-Qiang
authors.initials: JQ
authors.lastname: Guo
authors.affiliation: Department of Respiratory Medicine, The Affiliated Municipal Hospital of Xuzhou Medical College, Xuzhou, Jiangsu, 221002, People's Republic of China.
authors.forename: Shu-Ling
authors.initials: SL
authors.lastname: Wang
authors.affiliation: Jiangsu Key Laboratory of Brain Disease Bioinformation, Xuzhou Medical College, Xuzhou, Jiangsu, 221004, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Anatomy, Xuzhou Medical College, Xuzhou, Jiangsu, 221004, People's Republic of China.
authors.forename: Yu-Lan
authors.initials: YL
authors.lastname: Wang
authors.affiliation: Jiangsu Key Laboratory of Brain Disease Bioinformation, Xuzhou Medical College, Xuzhou, Jiangsu, 221004, People's Republic of China. biosongyuanjian@126.com.|Department of Genetics, Research Center for Neurobiology, Xuzhou Medical College, 209 Tongshan Road, Xuzhou, Jiangsu, 221004, People's Republic of China. biosongyuanjian@126.com.
authors.forename: Yuan-Jian
authors.initials: YJ
authors.lastname: Song
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001572:Benzofurans / Q000737:chemistry / Q000494:pharmacology*; D002545:Brain Ischemia / Q000188:drug therapy* / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D008297:Male; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D001572:Benzofurans; C027125:3-n-butylphthalide; D048031:JNK Mitogen-Activated Protein Kinases; C505410:Casp3 protein, rat; D053148:Caspase 3
keywords: Butylphthalide; Caspase3; Ischemia/reperfusion; JNK