title: HMGB1 Osteo-Modulatory Action on Osteosarcoma SaOS-2 Cell Line: An Integrated Study From Biochemical and -Omics Approaches.
abstract: High mobility group box protein-1 (HMGB1) is released from cells under various pathological conditions and it plays a pivotal role as an alarmin signaling tissue damage. Little is known about the impact of HMGB1 in bone repair and remodeling. To this aim, we focused on HMGB1-induced effects on the in vitro osteoblast model SaOS-2. Cell proliferation was stimulated with a maximum at concentration of 2.5 nM, and such a dose also stimulated cell migration and scratch wound healing. We then characterized the modulatory effect of HMGB1 on bone biology, by using osteogenesis/mineralization assays, a PCR array, and the analysis of a series of osteogenic markers. We performed also a proteomic screening using SWATH-MS on SaOS-2 cell exposed to HMGB1 and we provide evidence for proteins modulated in HMGB1 exposed cells. Taken together, our data demonstrate that SaOS-2 cell proliferation, migration, and osteogenic differentiation were increased by HMGB1. We, therefore, propose that HMGB1 could be a potent bone-remodeling signal but the physiological meaning of this property remains to be more ascertained. J. Cell. Biochem. 117: 2559-2569, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: DiSIT-Dipartimento di Scienze e Innovazione Tecnologica, University of Piemonte Orientale, Viale Teresa Michel 11, Alessandria 15121, Italy. simona.martinotti@uniupo.it.
authors.forename: Simona
authors.initials: S
authors.lastname: Martinotti
authors.affiliation: DiSIT-Dipartimento di Scienze e Innovazione Tecnologica, University of Piemonte Orientale, Viale Teresa Michel 11, Alessandria 15121, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Patrone
authors.affiliation: DiSIT-Dipartimento di Scienze e Innovazione Tecnologica, University of Piemonte Orientale, Viale Teresa Michel 11, Alessandria 15121, Italy.|Isalit srl, sede legale-via Bovio, 6, Novara; sede operativa-Politecnico di Torino, sede di Alessandria, viale Teresa Michel 5, Alessandria 15121, Italy.
authors.forename: Marcello
authors.initials: M
authors.lastname: Manfredi
authors.affiliation: DiSIT-Dipartimento di Scienze e Innovazione Tecnologica, University of Piemonte Orientale, Viale Teresa Michel 11, Alessandria 15121, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Gosetti
authors.affiliation: Department of Experimental Medicine (DIMES)-Biochemistry Section, University of Genova, Viale Benedetto XV 1, Genova 16132, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Pedrazzi
authors.affiliation: DiSIT-Dipartimento di Scienze e Innovazione Tecnologica, University of Piemonte Orientale, Viale Teresa Michel 11, Alessandria 15121, Italy.
authors.forename: Emilio
authors.initials: E
authors.lastname: Marengo
authors.affiliation: DiSIT-Dipartimento di Scienze e Innovazione Tecnologica, University of Piemonte Orientale, Viale Teresa Michel 11, Alessandria 15121, Italy.
authors.forename: Elia
authors.initials: E
authors.lastname: Ranzato
mesh_terms: D015153:Blotting, Western; D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D016723:Bone Remodeling; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D024243:HMGB1 Protein / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D014407:Tumor Cells, Cultured; D014945:Wound Healing
chemical_list: D024243:HMGB1 Protein; D020543:Proteome; D012333:RNA, Messenger
keywords: HMGB1; MIGRATION; OSTEOGENESIS; PCR ARRAY; SCRATCH WOUND ASSAY; SWATH-MS; SaOS-2