title: Early tissue formation on whole-area osteochondral defect of rabbit patella by covering with fibroin sponge.
abstract: Large osteochondral defects have been difficult to repair via tissue engineering treatments due to the lack of a sufficient number of source cells for repairing the defect and to the severe mechanical stresses affecting the replacement tissue. In the present study, whole-area osteochondral defects of rabbit patella were covered and wrapped with a fibroin sponge containing chondrocytes, with or without Green Fluorescent Protein (GFP) transgenic marking, on the surface facing the osteochondral defect. Five of eight osteochondral defects that were covered with the chondrocyte-seeded fibroin sponges showed hyaline cartilage-like repair containing no fibroin fragments at 6 weeks after surgery. The repaired tissue showed a layer formation, which showed intensive safranin-O and toluidine blue staining, and which showed positive type II collagen immunostaining. The average surface coverage of the repaired cartilage was 53%. On average, 48% of the cells in the repaired tissue were derived from GFP transgenic chondrocytes, which had been seeded in the fibroin sponge. The fibroin-sponge covering had the potential to allow the early repair of large osteochondral defects. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1474-1482, 2016.
authors.affiliation: Field of Medical Engineering, Division of Mechanical Engineering and Science Graduate School of Engineering, Kyoto University, Kyoto, Japan. heihou@mb.infoweb.ne.jp.|Department of Plastic Surgery, Kyoto University Postgraduate School of Medicine, Kyoto, Japan. heihou@mb.infoweb.ne.jp.
authors.forename: Eiichi
authors.initials: E
authors.lastname: Hirakata
authors.affiliation: Field of Medical Engineering, Division of Mechanical Engineering and Science Graduate School of Engineering, Kyoto University, Kyoto, Japan.
authors.forename: Naohide
authors.initials: N
authors.lastname: Tomita
authors.affiliation: National Institute of Agrobiological Sciences, Ibaraki, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Tamada
authors.affiliation: Institute of Medical Science, Tokyo Medical University, Tokyo, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Suguro
authors.affiliation: Department of Physical Therapy, School of Health Science and Social Welfare, Kibi International University, Okayama, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Nakajima
authors.affiliation: Field of Medical Engineering, Division of Mechanical Engineering and Science Graduate School of Engineering, Kyoto University, Kyoto, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kambe
authors.affiliation: Field of Medical Engineering, Division of Mechanical Engineering and Science Graduate School of Engineering, Kyoto University, Kyoto, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Yamada
authors.affiliation: Field of Medical Engineering, Division of Mechanical Engineering and Science Graduate School of Engineering, Kyoto University, Kyoto, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Yamamoto
authors.affiliation: Field of Medical Engineering, Division of Mechanical Engineering and Science Graduate School of Engineering, Kyoto University, Kyoto, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kawakami
authors.affiliation: Field of Medical Engineering, Division of Mechanical Engineering and Science Graduate School of Engineering, Kyoto University, Kyoto, Japan.
authors.forename: Akihisa
authors.initials: A
authors.lastname: Otaka
authors.affiliation: Department of Orthopaedic Surgery, Rakuyo Hospital, Kyoto, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Okumura
authors.affiliation: Department of Plastic Surgery, Kyoto University Postgraduate School of Medicine, Kyoto, Japan.
authors.forename: Shigehiko
authors.initials: S
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D019902:Chondrocytes / Q000378:metabolism / Q000473:pathology / Q000637:transplantation; D005349:Fibroins / Q000494:pharmacology*; D010329:Patella / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D011817:Rabbits
chemical_list: D005349:Fibroins
keywords: cartilage repair; cell delivery; fibroin sponge; osteoarthritis; osteochondral defect