title: Matrix Gla protein repression by miR-155 promotes oncogenic signals in breast cancer MCF-7 cells.
abstract: MGP is a protein that was initially associated with the inhibition of calcification in skeleton, soft tissues, and arteries, but more recently also implicated in cancer. In breast cancer, higher levels of MGP mRNA were associated with poor prognosis, but since this deregulation was never demonstrated at the protein level, we postulated the involvement of a post-transcriptional regulatory mechanism. In this work we show that MGP is significantly repressed by miR-155 in breast cancer MCF-7 cells, and concomitantly there is a stimulation of cell proliferation and cell invasiveness. This study brings new insights into the putative involvement of MGP and oncomiR-155 in breast cancer, and may contribute to develop new therapeutic strategies.
authors.affiliation: Centre of Marine Sciences (CCMAR), University of Algarve, Faro, Portugal.
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Tiago
authors.affiliation: Centre of Marine Sciences (CCMAR), University of Algarve, Faro, Portugal.
authors.forename: Natércia
authors.initials: N
authors.lastname: Conceição
authors.affiliation: Centre of Marine Sciences (CCMAR), University of Algarve, Faro, Portugal.
authors.forename: Helena
authors.initials: H
authors.lastname: Caiado
authors.affiliation: Centre of Marine Sciences (CCMAR), University of Algarve, Faro, Portugal.
authors.forename: Vincent
authors.initials: V
authors.lastname: Laizé
authors.affiliation: Centre of Marine Sciences (CCMAR), University of Algarve, Faro, Portugal.|Department of Biomedical Sciences and Medicine, University of Algarve, Faro, Portugal.
authors.forename: Maria Leonor
authors.initials: ML
authors.lastname: Cancela
mesh_terms: D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D063646:Carcinogenesis / Q000235:genetics*; D049109:Cell Proliferation; D016326:Extracellular Matrix Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D057809:HEK293 Cells; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D009361:Neoplasm Invasiveness; D034741:RNA, Small Interfering / Q000378:metabolism; D015398:Signal Transduction; D000097568:Matrix Gla Protein
chemical_list: D002135:Calcium-Binding Proteins; D016326:Extracellular Matrix Proteins; D035683:MicroRNAs; D034741:RNA, Small Interfering; D000097568:Matrix Gla Protein; C524942:MIRN155 microRNA, human
keywords: MCF-7 cells; MGP; breast cancer; miR-155; oncogenesis