title: Differential regulation of T-cell dependent and T-cell independent antibody responses through arginine methyltransferase PRMT1 in vivo.
abstract: Protein arginine methyltransferase 1 (PRMT1), a major PRMT in mammalian cells, has been shown to play a crucial role in multiple biological functions in vitro. To explore the role of PRMT1 in B cells in vivo, we generated B cell-specific PRMT1-deficient (Prmt1(-/-) ) mice using a Cre-loxP system. Prmt1(-/-) mice showed a defect in B-cell development with diminished levels of serum antibodies. Antibody responses in Prmt1(-/-) mice were absent after stimulation with the type 2 T cell-independent antigen NP-Ficoll but intact after stimulation with the T cell-dependent antigen NP-OVA. Our findings comprise the first evidence showing that PRMT1 is necessary for lymphocyte functions in vivo.
authors.affiliation: Department of Immunology, Tokyo Medical University, Japan.
authors.forename: Kikumi
authors.initials: K
authors.lastname: Hata
authors.affiliation: Department of Immunology, Tokyo Medical University, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Yanase
authors.affiliation: Animal Research Center, Tokyo Medical University, Japan.
authors.forename: Katsuko
authors.initials: K
authors.lastname: Sudo
authors.affiliation: Animal Resource Development Unit, RIKEN Center for Life Science Technologies, Kobe, Japan.|Genetic Engineering Team, RIKEN Center for Life Science Technologies, Kobe, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kiyonari
authors.affiliation: Genetic Engineering Team, RIKEN Center for Life Science Technologies, Kobe, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Mukumoto
authors.affiliation: Department of Immunology, Tokyo Medical University, Japan.
authors.forename: Junichiro
authors.initials: J
authors.lastname: Mizuguchi
authors.affiliation: Department of Immunology, Tokyo Medical University, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yokosuka
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000276:immunology*; D000941:Antigens / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D005362:Ficoll / Q000276:immunology; D007136:Immunoglobulins / Q000097:blood; D008213:Lymphocyte Activation; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010047:Ovalbumin / Q000276:immunology; D011484:Protein-Arginine N-Methyltransferases / Q000172:deficiency / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000941:Antigens; D007136:Immunoglobulins; D005362:Ficoll; D010047:Ovalbumin; C546338:Prmt1 protein, mouse; D011484:Protein-Arginine N-Methyltransferases
keywords: B cells; PRMT; T-dependent antigen; T-independent antigen; antibody response; arginine methylation