title: Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi.
abstract: CS5931 is a novel anticancer agent isolated from the sea squirt Ciona savignyi. However, its content in the species is very low, and developing a novel approach for production of the polypeptide is promising. In the present study, we expressed and purified the polypeptide from E. coli, and the fermentation conditions were studied using response surface methodology. The yield of CS5931 was increased from 2.0 to 7.5 mg/L. The denaturing and renaturation conditions were also studied. Using the optimized renaturation condition, the anticancer activity of refolding CS5931 was increased significantly; the value of IC50 was decreased from 23.2 to 11.6 μM. In vivo study using xenograft nude mice bearing HCT116 cancer cells revealed that CS5931 was able to inhibit the growth of tumor significantly. The study provides a useful approach for obtaining enough amount of CS5931 for further study. This study is also important for developing the polypeptide as a novel anticancer agent.
authors.affiliation: School of Pharmacy, Tianjin Medical University, Tianjin 300070, China. chenxiaoshuang2012@126.com.
authors.forename: Xiaoshuang
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Pharmacolgoy, Capital Medical University, 10 Xitoutiao, Youanmen St., Beijing 100069, China. xhl52@msn.com.
authors.forename: Huanli
authors.initials: H
authors.lastname: Xu
authors.affiliation: Linqu, Haifushan Hospital, Shandong 262600, China. libo991@126.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.affiliation: Linqu, Haifushan Hospital, Shandong 262600, China. wangfeng9911@126.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Pharmacolgoy, Capital Medical University, 10 Xitoutiao, Youanmen St., Beijing 100069, China. chen911_1@163.com.
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Chen
authors.affiliation: School of Pharmacy, Tianjin Medical University, Tianjin 300070, China. kongdexin@tijmu.edu.cn.
authors.forename: Dexin
authors.initials: D
authors.lastname: Kong
authors.affiliation: Department of Pharmacolgoy, Capital Medical University, 10 Xitoutiao, Youanmen St., Beijing 100069, China. linxiukun@lzmc.edu.cn.
authors.forename: Xiukun
authors.initials: X
authors.lastname: Lin
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D005285:Fermentation; D000077155:Granulins; D045325:HCT116 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D036341:Intercellular Signaling Peptides and Proteins / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D008819:Mice, Nude; D014561:Urochordata / Q000378:metabolism*; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D000077155:Granulins; D036341:Intercellular Signaling Peptides and Proteins
keywords: CS5931; antitumor activity; protein expression; renaturation