title: Oral immunization of mice against Clostridium perfringens epsilon toxin with a Lactobacillus casei vector vaccine expressing epsilon toxoid.
abstract: Clostridium perfringens type D infects ruminants and causes the enterotoxemia disease by ε-toxin. A mutated ε-toxin gene lacking toxicity was designed, synthesized, and cloned into the pT1NX vector and electroporated into Lactobacillus casei competent cells to yield LC-pT1NX-ε recombinant strain. BALB/c mice, immunized orally with this strain, highly induced mucosal, humoral, and cell-mediated immune responses and developed a protection against 200 MLD/ml of the activated ε-toxin. This study showed that the LC-pT1NX-ε could be a promising vaccine candidate against the enterotoxemia disease.
authors.affiliation: Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran; Department of Anaerobic Bacterial Vaccine Research and Production, Razi Vaccine and Serum Research Institute- Kerman branch, Kerman, Iran.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Alimolaei
authors.affiliation: Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran. Electronic address: golchin@uk.ac.ir.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Golchin
authors.affiliation: Department of Immunology, Medical School of Kerman University, Kerman, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Daneshvar
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D003016:Clostridium perfringens / Q000235:genetics / Q000276:immunology*; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005738:Gas Gangrene / Q000097:blood / Q000276:immunology / Q000401:mortality / Q000517:prevention & control*; D023061:Gene Order; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D007114:Immunization; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007780:Lacticaseibacillus casei / Q000235:genetics / Q000276:immunology*; D051379:Mice; D014121:Toxoids / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; C039811:Clostridium perfringens epsilon-toxin; D016207:Cytokines; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D014121:Toxoids
keywords: Clostridium perfringens; Enterotoxemia; Lactobacillus casei; Toxoid; Vaccine