title: Antibacterial Effects of Liposomes Containing Phospholipid Cardiolipin and Fluoroquinolone Levofloxacin on Mycobacterium tuberculosis with Extensive Drug Resistance.
abstract: The effects of liposomes containing phospholipid cardiolipin without antibiotic and loaded with levofloxacin on the growth of Mycobacterium tuberculosis with extensive drug resistance were studied in vitro. Liposomes consisting of cardiolipin alone in a concentration of 335 μM completely suppressed the growth of M. tuberculosis. In order to reduce the minimum inhibitory concentration of cardiolipin, complex liposome preparation consisting of phosphatidylcholin/cholesterol/cardiolipin and loaded with levofloxacin was prepared. Due to this, the cardiolipin concentration was reduced to 33.5 μM (50 μg/ml) and concentration of levofloxacin - to 2 μg/ml.
authors.affiliation: M. V. Lomonosov Moscow State University of Fine Chemical Technologies, Moscow, Russia.
authors.forename: S K
authors.initials: SK
authors.lastname: Gaidukevich
authors.affiliation: M. V. Lomonosov Moscow State University of Fine Chemical Technologies, Moscow, Russia.
authors.forename: Yu L
authors.initials: YL
authors.lastname: Mikulovich
authors.affiliation: Central Research Institute of Tuberculosis, Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: T G
authors.initials: TG
authors.lastname: Smirnova
authors.affiliation: Central Research Institute of Tuberculosis, Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: S N
authors.initials: SN
authors.lastname: Andreevskaya
authors.affiliation: M. V. Lomonosov Moscow State University of Fine Chemical Technologies, Moscow, Russia. galinams@yandex.ru.
authors.forename: G M
authors.initials: GM
authors.lastname: Sorokoumova
authors.affiliation: Central Research Institute of Tuberculosis, Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: L N
authors.initials: LN
authors.lastname: Chernousova
authors.affiliation: M. V. Lomonosov Moscow State University, Moscow, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Selishcheva
authors.affiliation: M. V. Lomonosov Moscow State University of Fine Chemical Technologies, Moscow, Russia.
authors.forename: V I
authors.initials: VI
authors.lastname: Shvets
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002308:Cardiolipins / Q000494:pharmacology*; D002784:Cholesterol / Q000494:pharmacology; D004337:Drug Carriers / Q000378:metabolism* / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial; D024841:Fluoroquinolones / Q000494:pharmacology*; D064704:Levofloxacin / Q000494:pharmacology*; D008081:Liposomes / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D010713:Phosphatidylcholines / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D002308:Cardiolipins; D004337:Drug Carriers; D024841:Fluoroquinolones; D008081:Liposomes; D010713:Phosphatidylcholines; D064704:Levofloxacin; D002784:Cholesterol
keywords: Mycobacterium tuberculosis; bacteriostatic effect; cardiolipin; levofloxacin; resistance