title: Structural Influences: Cholesterol, Drug, and Proton Binding to Full-Length Influenza A M2 Protein.
abstract: The structure and functions of the M2 protein from Influenza A are sensitive to pH, cholesterol, and the antiinfluenza drug Amantadine. This is a tetrameric membrane protein of 97 amino-acid residues that has multiple functions, among them as a proton-selective channel and facilitator of viral budding, replacing the need for the ESCRT proteins that other viruses utilize. Here, various amino-acid-specific-labeled samples of the full-length protein were prepared and mixed, so that only interresidue (13)C-(13)C cross peaks between two differently labeled proteins representing interhelical interactions are observed. This channel is activated at slightly acidic pH values in the endosome when the His(37) residues in the middle of the transmembrane domain take on a +2 or +3 charged state. Changes observed here in interhelical distances in the N-terminus can be accounted for by modest structural changes, and no significant changes in structure were detected in the C-terminal portion of the channel upon activation of the channel. Amantadine, which blocks proton conductance by binding in the aqueous pore near the N-terminus, however, significantly modifies the tetrameric structure on the opposite side of the membrane. The interactions between the juxtamembrane amphipathic helix of one monomer and its neighboring monomer observed in the absence of drug are disrupted in its presence. However, the addition of cholesterol prevents this structural disruption. In fact, strong interactions are observed between cholesterol and residues in the amphipathic helix, accounting for cholesterol binding adjacent to a native palmitoylation site and near to an interhelix crevice that is typical of cholesterol binding sites. The resultant stabilization of the amphipathic helix deep in the bilayer interface facilitates the bilayer curvature that is essential for viral budding.
authors.affiliation: Department of Chemistry and Biochemistry, Florida State University, Tallahassee, Florida; National High Magnetic Field Lab, Florida State University, Tallahassee, Florida.
authors.forename: E Vindana
authors.initials: EV
authors.lastname: Ekanayake
authors.affiliation: National High Magnetic Field Lab, Florida State University, Tallahassee, Florida.
authors.forename: Riqiang
authors.initials: R
authors.lastname: Fu
authors.affiliation: Department of Chemistry and Biochemistry, Florida State University, Tallahassee, Florida; National High Magnetic Field Lab, Florida State University, Tallahassee, Florida; Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida. Electronic address: cross@magnet.fsu.edu.
authors.forename: Timothy A
authors.initials: TA
authors.lastname: Cross
mesh_terms: D000547:Amantadine / Q000378:metabolism*; D000595:Amino Acid Sequence; D002784:Cholesterol / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009980:Influenza A virus / Q000378:metabolism*; D000072417:Protein Domains; D017433:Protein Structure, Secondary; D011522:Protons; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000378:metabolism*; D000086082:Viroporin Proteins
chemical_list: D000547:Amantadine; D002784:Cholesterol; D011522:Protons; D014763:Viral Matrix Proteins; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins