title: Quantitative Probing of Cu(2+) Ions Naturally Present in Single Living Cells.
abstract: Quantitative probing of Cu(2+) ions naturally present in single living cells is realized by developing a quantum-dot-embedded nanowire-waveguide probe. The intracellular Cu(2+) ion concentration is quantified by direct monitoring of photoluminescence quenching during the insertion of the nanowire in a living neuron. The measured intracellular Cu(2+) ion concentration is 3.34 ± 1.04 × 10(-6) m (mean ± s.e.m.) in single hippocampal neurons.
authors.affiliation: X-Ray Imaging Center, Department of Materials Science and Engineering, Pohang University of Science and Technology, Pohang, 790-784, Korea.
authors.forename: Junho
authors.initials: J
authors.lastname: Lee
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, 790-784, Korea.
authors.forename: Hwa-Rim
authors.initials: HR
authors.lastname: Lee
authors.affiliation: X-Ray Imaging Center, Department of Materials Science and Engineering, Pohang University of Science and Technology, Pohang, 790-784, Korea.
authors.forename: Jaeyeon
authors.initials: J
authors.lastname: Pyo
authors.affiliation: Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang, 790-784, Korea.
authors.forename: Youngseob
authors.initials: Y
authors.lastname: Jung
authors.affiliation: Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang, 790-784, Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Seo
authors.affiliation: Department of Life Sciences, Pohang University of Science and Technology, Pohang, 790-784, Korea.
authors.forename: Hye Guk
authors.initials: HG
authors.lastname: Ryu
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, 790-784, Korea.|Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang, 790-784, Korea.|Department of Life Sciences, Pohang University of Science and Technology, Pohang, 790-784, Korea.
authors.forename: Kyong-Tai
authors.initials: KT
authors.lastname: Kim
authors.affiliation: X-Ray Imaging Center, Department of Materials Science and Engineering, Pohang University of Science and Technology, Pohang, 790-784, Korea.
authors.forename: Jung Ho
authors.initials: JH
authors.lastname: Je
mesh_terms: D002413:Cations, Divalent; D003300:Copper / Q000737:chemistry*; D053770:Nanowires; D045663:Quantum Dots
chemical_list: D002413:Cations, Divalent; D003300:Copper
keywords: PL quenching; active nanowires; intracellular copper ions; single living cells; waveguide probes