title: A microfluidic array for real-time live-cell imaging of human and rodent pancreatic islets.
abstract: In this study, we present a microfluidic array for high-resolution imaging of individual pancreatic islets. The device is based on hydrodynamic trapping principle and enables real-time analysis of islet cellular responses to insulin secretagogues. This device has significant advantages over our previously published perifusion chamber device including significantly increased analytical power and assay sensitivity, as well as improved spatiotemporal resolution. The islet array, with live-cell multiparametric imaging integration, provides a better tool to understand the physiological and pathophysiological changes of pancreatic islets through the analysis of single islet responses. This platform demonstrates the feasibility of array-based islet cellular analysis and opens up a new modality to conduct informative and quantitive evaluation of islets and cell-based screening for new diabetes treatments.
authors.affiliation: Department of Surgery/Transplant, University of Illinois at Chicago, Chicago, IL, 60612 USA. jober@uic.edu wangy@uic.edu and Department of Bioengineering, University of Illinois at Chicago, Chicago, IL, 60607 USA.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Nourmohammadzadeh
authors.affiliation: Department of Surgery/Transplant, University of Illinois at Chicago, Chicago, IL, 60612 USA. jober@uic.edu wangy@uic.edu and Department of Bioengineering, University of Illinois at Chicago, Chicago, IL, 60607 USA.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Xing
authors.affiliation: Department of Electroengineering, Northern Illinois University, Chicago, IL, 60115 USA.
authors.forename: Jin Wuk
authors.initials: JW
authors.lastname: Lee
authors.affiliation: Department of Surgery/Transplant, University of Illinois at Chicago, Chicago, IL, 60612 USA. jober@uic.edu wangy@uic.edu and Department of Bioengineering, University of Illinois at Chicago, Chicago, IL, 60607 USA.
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Bochenek
authors.affiliation: Department of Surgery/Transplant, University of Illinois at Chicago, Chicago, IL, 60612 USA. jober@uic.edu wangy@uic.edu and Department of Bioengineering, University of Illinois at Chicago, Chicago, IL, 60607 USA.
authors.forename: Joshua E
authors.initials: JE
authors.lastname: Mendoza-Elias
authors.affiliation: Department of Surgery/Transplant, University of Illinois at Chicago, Chicago, IL, 60612 USA. jober@uic.edu wangy@uic.edu.
authors.forename: James J
authors.initials: JJ
authors.lastname: McGarrigle
authors.affiliation: Department of Surgery/Transplant, University of Illinois at Chicago, Chicago, IL, 60612 USA. jober@uic.edu wangy@uic.edu.
authors.forename: Enza
authors.initials: E
authors.lastname: Marchese
authors.affiliation: Department of Surgery/Transplant, University of Illinois at Chicago, Chicago, IL, 60612 USA. jober@uic.edu wangy@uic.edu.
authors.forename: Yeh
authors.initials: Y
authors.lastname: Chun-Chieh
authors.affiliation: Department of Bioengineering, University of Illinois at Chicago, Chicago, IL, 60607 USA.
authors.forename: David T
authors.initials: DT
authors.lastname: Eddington
authors.affiliation: Department of Surgery/Transplant, University of Illinois at Chicago, Chicago, IL, 60612 USA. jober@uic.edu wangy@uic.edu and Department of Bioengineering, University of Illinois at Chicago, Chicago, IL, 60607 USA.
authors.forename: José
authors.initials: J
authors.lastname: Oberholzer
authors.affiliation: Department of Surgery/Transplant, University of Illinois at Chicago, Chicago, IL, 60612 USA. jober@uic.edu wangy@uic.edu and Department of Bioengineering, University of Illinois at Chicago, Chicago, IL, 60607 USA.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D002470:Cell Survival; D005240:Feasibility Studies; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology*; D056656:Lab-On-A-Chip Devices; D051379:Mice; D057054:Molecular Imaging / Q000295:instrumentation*