title: The Eukaryotic Replisome Goes Under the Microscope.
abstract: The machinery at the eukaryotic replication fork has seen many new structural advances using electron microscopy and crystallography. Recent structures of eukaryotic replisome components include the Mcm2-7 complex, the CMG helicase, DNA polymerases, a Ctf4 trimer hub and the first look at a core replisome of 20 different proteins containing the helicase, primase, leading polymerase and a lagging strand polymerase. The eukaryotic core replisome shows an unanticipated architecture, with one polymerase sitting above the helicase and the other below. Additionally, structures of Mcm2 bound to an H3/H4 tetramer suggest a direct role of the replisome in handling nucleosomes, which are important to DNA organization and gene regulation. This review provides a summary of some of the many recent advances in the structure of the eukaryotic replisome.
authors.affiliation: DNA Replication Lab, The Rockefeller University, 1230 York Avenue, New York, New York, USA; Howard Hughes Medical Institute. Electronic address: odonnel@rockefeller.edu.
authors.forename: Mike
authors.initials: M
authors.lastname: O'Donnell
authors.affiliation: Department of Biochemistry & Cell Biology, Stony Brook University, Stony Brook, New York, USA; Biology Department, Brookhaven National Laboratory, Upton, New York, USA. Electronic address: hli@bnl.gov.
authors.forename: Huilin
authors.initials: H
authors.lastname: Li
mesh_terms: D000818:Animals; D003461:Crystallography; D004265:DNA Helicases / Q000737:chemistry / Q000378:metabolism; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry / Q000378:metabolism*; D008854:Microscopy, Electron; D064110:Minichromosome Maintenance Proteins / Q000737:chemistry* / Q000378:metabolism; D009707:Nucleosomes / Q000737:chemistry / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: C078338:CTF4 protein, S cerevisiae; D004268:DNA-Binding Proteins; D009707:Nucleosomes; D029701:Saccharomyces cerevisiae Proteins; D004259:DNA-Directed DNA Polymerase; D004265:DNA Helicases; C069199:MCM2 protein, S cerevisiae; D064110:Minichromosome Maintenance Proteins