title: Development of 14 microsatellite markers in Odontites vernus s.l. (Orobanchaceae) and cross-amplification in related taxa.
abstract: OBJECTIVE
  Microsatellite primers were developed for the first time in the root hemiparasite herb Odontites vernus (Orobanchaceae). These markers will be useful to investigate the role of polyploidization in the evolution of this diploid-tetraploid complex, as well as the extent of gene flow between different ploidy levels.
  
  
  RESULTS
  Fourteen polymorphic and reproducible loci were identified and optimized from O. vernus using a microsatellite-enriched library and 454 Junior sequencing. The set of primers amplified di- to pentanucleotide repeats and showed two to 13 alleles per locus. Transferability was tested in 30 taxa (19 belonging to Odontites and 11 from eight other genera of Orobanchaceae tribe Rhinantheae).
  
  
  CONCLUSIONS
  The results indicate the utility of the newly developed microsatellites in O. vernus and several other species, which will be useful for taxon delimitation and conservation genetics studies.
authors.affiliation: Departamento de Botánica, Universidad de Salamanca, E-37007 Salamanca, Spain; Biobanco de ADN Vegetal, Banco Nacional de ADN, Edificio Multiusos I+D+I, E-37007 Salamanca, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Pinto-Carrasco
authors.affiliation: Faculty of Science, University of South Bohemia, CZ-370 05 České Budějovice, Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Košnar
authors.affiliation: Departamento de Botánica, Universidad de Salamanca, E-37007 Salamanca, Spain; Biobanco de ADN Vegetal, Banco Nacional de ADN, Edificio Multiusos I+D+I, E-37007 Salamanca, Spain.
authors.forename: Noemí
authors.initials: N
authors.lastname: López-González
authors.affiliation: Faculty of Science, University of South Bohemia, CZ-370 05 České Budějovice, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Koutecký
authors.affiliation: Faculty of Science, University of South Bohemia, CZ-370 05 České Budějovice, Czech Republic.
authors.forename: Jakub
authors.initials: J
authors.lastname: Těšitel
authors.affiliation: Departamento de Botánica, Universidad de Salamanca, E-37007 Salamanca, Spain; Biobanco de ADN Vegetal, Banco Nacional de ADN, Edificio Multiusos I+D+I, E-37007 Salamanca, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Rico
authors.affiliation: Departamento de Botánica, Universidad de Salamanca, E-37007 Salamanca, Spain; Biobanco de ADN Vegetal, Banco Nacional de ADN, Edificio Multiusos I+D+I, E-37007 Salamanca, Spain.
authors.forename: M Montserrat
authors.initials: MM
authors.lastname: Martínez-Ortega
keywords: Odontites vernus; Orobanchaceae; Rhinantheae; conservation; diploid-tetraploid complex; microsatellite