title: [Measles virus stimulation effect on the expression of Toll-like receptors in PBMC].
abstract: BACKGROUND
  Toll-like receptors (TLRs) are an important component of a innate immune system. Stimulation of TLRs, through action with helper T cells, could change Th1/Th2 balance and thus affect adaptive immune response. Receptors TLR2 and TLR4 play important role in immune response to measles virus. The aim of this work was stimulation of the peripheral blood mononuclear cells (PBMC) and determination of Toll-like gene expression in these cells.
  
  
  METHODS
  PBMCs from 20 healthy donors were stimulated with measles viruses and ligands for TLR2 and TLR4. For examinations the real time RT-PCR (QuantiFast® Assay, Qiagen) was used. The expression of Toll-like receptors was determined on muRNA level, using real-time one step RT-PCR (QuantiFast® Assay, Qiagen) with simultaneous detection of TLR2 and TLR4 genes and housekeeping gene (GAPDH).
  
  
  RESULTS
  Virus-specific influence of wild measles virus strains activity on PBMC derived from vaccinated seronegative individuals manifested in higher level of expression of TLR2 and TLR4 genes in compare to the expression of these genes in PBMC of seropositive individuals.
  
  
  CONCLUSIONS
  Toll-like receptors participate in the development of immune response to measles virus.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Częścik
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Trzcińska
authors.forename: Milena
authors.initials: M
authors.lastname: Dunal-Szczepaniak
authors.forename: Joanna
authors.initials: J
authors.lastname: Siennicka
mesh_terms: D005786:Gene Expression Regulation; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D008459:Measles virus / Q000276:immunology*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D051195:Toll-Like Receptor 2 / Q000235:genetics* / Q000276:immunology; D051197:Toll-Like Receptor 4 / Q000235:genetics* / Q000276:immunology
chemical_list: D012333:RNA, Messenger; C495342:TLR2 protein, human; C495345:TLR4 protein, human; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4