title: Simultaneous Quantitation of Free Amino Acids, Nucleosides and Nucleobases in Sipunculus nudus by Ultra-High Performance Liquid Chromatography with Triple Quadrupole Mass Spectrometry.
abstract: To evaluate the nutritional and functional value of Sipunculus nudus, a rapid, simple and sensitive analytical method was developed using ultra-high performance liquid chromatography coupled with a triple quadrupole mass detection in multiple-reaction monitoring mode for the simultaneous quantitative determination of 25 free amino acids and 16 nucleosides and nucleobases in S. nudus within 20 min, which was confirmed to be reproducible and accurate. The limits of detection (LODs) and quantification (LOQs) were between 0.003-0.229 μg/mL and 0.008-0.763 μg/mL for the 41 analytes, respectively. The established method was applied to analyze 19 batches of S. nudus samples from four habitats with two different processing methods. The results showed that S. nudus contained a variety of free amino acids, nucleosides and nucleobases in sufficient quantity and reasonable proportion. They also demonstrated that the contents of these compounds in different parts of S. nudus were significantly discriminating, which were in the order: (highest) coelomic fluid > body wall > intestine (lowest). The method is simple and accurate, and could serve as a technical support for establishing quality control of S. nudus and other functional seafoods. Moreover, the research results also laid foundation for further exploitation and development of S. nudus.
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. geyahui_jsnt@163.com.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Ge
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. yupingtang@njucm.edu.cn.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. gsh916@gmail.com.
authors.forename: Sheng
authors.initials: S
authors.lastname: Guo
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. liuxin_njutcm@163.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. 18913133908@163.com.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. lizhang2020@126.com.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. peiliu1981@126.com.
authors.forename: Pei
authors.initials: P
authors.lastname: Liu
authors.affiliation: College of Ocean and Environment, Xiamen University, Xiamen 361005, China. sxding@xmu.edu.cn.
authors.forename: Shaoxiong
authors.initials: S
authors.lastname: Ding
authors.affiliation: Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China. xzlin@tio.org.cn.
authors.forename: Xiangzhi
authors.initials: X
authors.lastname: Lin
authors.affiliation: Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China. linrulong@yahoo.com.
authors.forename: Rurong
authors.initials: R
authors.lastname: Lin
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. dja@njucm.edu.cn.
authors.forename: Jin-ao
authors.initials: JA
authors.lastname: Duan
mesh_terms: D000596:Amino Acids / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006571:Heterocyclic Compounds / Q000737:chemistry* / Q000302:isolation & purification; D057927:Hydrophobic and Hydrophilic Interactions; D009348:Nematoda / Q000737:chemistry*; D009705:Nucleosides / Q000737:chemistry* / Q000302:isolation & purification; D053719:Tandem Mass Spectrometry
chemical_list: D000596:Amino Acids; D006571:Heterocyclic Compounds; D009705:Nucleosides
keywords: Sipunculus nudus; UPLC-TQ-MS/MS; free amino acid; nucleobase; nucleoside