title: Adhesion and removal kinetics of Bacillus cereus biofilms on Ni-PTFE modified stainless steel.
abstract: Biofilm control remains a challenge to food safety. A well-studied non-fouling coating involves codeposition of polytetrafluoroethylene (PTFE) during electroless plating. This coating has been reported to reduce foulant build-up during pasteurization, but opportunities remain in demonstrating its efficacy in inhibiting biofilm formation. Herein, the initial adhesion, biofilm formation, and removal kinetics of Bacillus cereus on Ni-PTFE-modified stainless steel (SS) are characterized. Coatings lowered the surface energy of SS and reduced biofilm formation by > 2 log CFU cm(-2). Characterization of the kinetics of biofilm removal during cleaning demonstrated improved cleanability on the Ni-PTFE coated steel. There was no evidence of biofilm after cleaning by either solution on the Ni-PTFE coated steel, whereas more than 3 log and 1 log CFU cm(-2) of bacteria remained on the native steel after cleaning with water and an alkaline cleaner, respectively. This work demonstrates the potential application of Ni-PTFE non-fouling coatings on SS to improve food safety by reducing biofilm formation and improving the cleaning efficiency of food processing equipment.
authors.affiliation: a Department of Food Science , University of Massachusetts , Amherst, MA , USA.
authors.forename: Kang
authors.initials: K
authors.lastname: Huang
authors.affiliation: a Department of Food Science , University of Massachusetts , Amherst, MA , USA.
authors.forename: Lynne A
authors.initials: LA
authors.lastname: McLandsborough
authors.affiliation: a Department of Food Science , University of Massachusetts , Amherst, MA , USA.
authors.forename: Julie M
authors.initials: JM
authors.lastname: Goddard
mesh_terms: D001409:Bacillus cereus / Q000502:physiology*; D001422:Bacterial Adhesion; D058491:Bacterial Load; D018441:Biofilms; D003902:Detergents / Q000494:pharmacology; D007700:Kinetics; D011138:Polytetrafluoroethylene; D013193:Stainless Steel
chemical_list: D003902:Detergents; D013193:Stainless Steel; D011138:Polytetrafluoroethylene
keywords: Bacillus cereus; Nickel polytetrafluoroethylene (Ni-PTFE); biofilm; biofouling; fouling release coatings; non-fouling stainless steel