title: Anti-inflammatory Effect of Mesenchymal Stromal Cell Transplantation and Quercetin Treatment in a Rat Model of Experimental Cerebral Ischemia.
abstract: Here, we have investigated the synergistic effect of quercetin administration and transplantation of human umbilical cord mesenchymal stromal cells (HUMSCs) following middle cerebral artery occlusion in rat. Combining quercetin treatment with delayed transplantation of HUMSCs after local cerebral ischemia significantly (i) improved neurological functional recovery; (ii) reduced proinflammatory cytokines (interleukin(IL)-1β and IL-6), increased anti-inflammatory cytokines (IL-4, IL-10, and transforming growth factor-β1), and reduced ED-1 positive areas; (iii) inhibited cell apoptosis (caspase-3 expression); and (iv) improved the survival rate of HUMSCs in the injury site. Altogether, our results demonstrate that combined HUMSC transplantation and quercetin treatment is a potential strategy for reducing secondary damage and promoting functional recovery following cerebral ischemia.
authors.affiliation: Research Center of Clinic Medicine, Nanfang Hospital, Southern Medical University, No. 1838, Guangzhou Avenue North, Baiyun District, Guangzhou, 510515, China.
authors.forename: Lan-Lan
authors.initials: LL
authors.lastname: Zhang
authors.affiliation: The affiliated Bayi Brain Hospital, The Military General Hospital of Beijing, PLA, Beijing, 100700, China.
authors.forename: Hong-Tian
authors.initials: HT
authors.lastname: Zhang
authors.affiliation: The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong Province, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, China.
authors.forename: Ying-Qian
authors.initials: YQ
authors.lastname: Cai
authors.affiliation: Research Center of Clinic Medicine, Nanfang Hospital, Southern Medical University, No. 1838, Guangzhou Avenue North, Baiyun District, Guangzhou, 510515, China.
authors.forename: Yan-Jiang
authors.initials: YJ
authors.lastname: Han
authors.affiliation: Research Center of Clinic Medicine, Nanfang Hospital, Southern Medical University, No. 1838, Guangzhou Avenue North, Baiyun District, Guangzhou, 510515, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yao
authors.affiliation: Research Center of Clinic Medicine, Nanfang Hospital, Southern Medical University, No. 1838, Guangzhou Avenue North, Baiyun District, Guangzhou, 510515, China.
authors.forename: Zhao-Hu
authors.initials: ZH
authors.lastname: Yuan
authors.affiliation: Research Center of Clinic Medicine, Nanfang Hospital, Southern Medical University, No. 1838, Guangzhou Avenue North, Baiyun District, Guangzhou, 510515, China. bywu@smu.edu.cn.
authors.forename: Bing-Yi
authors.initials: BY
authors.lastname: Wu
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism / Q000628:therapy*; D016207:Cytokines / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D011794:Quercetin / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D016207:Cytokines; D015850:Interleukin-6; D016753:Interleukin-10; D015847:Interleukin-4; D011794:Quercetin
keywords: Apoptosis; Inflammation; Mesenchymal stromal cells; Quercetin; Stroke