title: The strengthening role of D:D interactions in fibrin self-assembly under oxidation.
abstract: The effect on ozone-induced oxidation on the self-assembly of fibrin in the presence of fibrin-stabilizing factor FXIIIa of soluble cross-linked fibrin oligomers was studied in a medium containing moderate urea concentrations. It is established that fibrin oligomers were formed by the protofibrils cross-linked through γ-γ dimers and the fibrils additionally cross-linked by through α-polymers. The oxidation promoted both the accumulation of greater amounts of γ-γ dimers and the formation of protofibrils, fibrils, and their dissociation products emerging with increasing urea concentrations, which have a high molecular weight. It is concluded that the oxidation enhances the axial interactions between D-regions of fibrin molecules.
authors.affiliation: Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 117977, Russia. markrosenfeld@rambler.ru.
authors.forename: M A
authors.initials: MA
authors.lastname: Rosenfeld
authors.affiliation: Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 117977, Russia.
authors.forename: V B
authors.initials: VB
authors.lastname: Leonova
authors.affiliation: Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 117977, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Bychkova
authors.affiliation: Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 117977, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Kostanova
authors.affiliation: Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 117977, Russia.
authors.forename: M I
authors.initials: MI
authors.lastname: Biryukova
authors.affiliation: Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 117977, Russia.
authors.forename: N B
authors.initials: NB
authors.lastname: Sultimova
authors.affiliation: Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 117977, Russia.
authors.forename: M L
authors.initials: ML
authors.lastname: Konstantinova
authors.affiliation: Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 117977, Russia.
authors.forename: M G
authors.initials: MG
authors.lastname: Gorobets
mesh_terms: D005176:Factor XIII / Q000737:chemistry / Q000378:metabolism; D005337:Fibrin / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D010084:Oxidation-Reduction; D011485:Protein Binding; D055503:Protein Multimerization; D014508:Urea / Q000737:chemistry
chemical_list: D014508:Urea; D005337:Fibrin; D005176:Factor XIII