title: Radioadaptive Response for Reproductive Cell Death Demonstrated in In Vivo Tissue Model of Caenorhabditis elegans.
abstract: Reproductive cell death (RCD) occurs after one or more cell divisions resulting from an insult such as radiation exposure or other treatments with carcinogens or mutagens. The radioadaptive response for RCD is usually investigated by in vitro or in vivo clonogenic assay. To date, this has not been demonstrated in the vulval tissue in Caenorhabditis elegans ( C. elegans ), which is a well established in vivo model for radiation-induced RCD. In this study to determine whether radioadaptive response occurs in the vulval tissue model of C. elegans , early larval worms were gamma irradiated with lower adaptive doses, followed by higher challenge doses. The ratio of protruding vulva was used to assess the RCD of vulval cells. The results of this study showed that the radioadaptive response for RCD in this vulval tissue model could be well induced by dose combinations of 5 + 75 Gy and 5 + 100 Gy at the time point of 14-16 h in worm development. In addition, the time course analysis indicated that radioresistance in vulval cells developed within 1.75 h after an adaptive dose and persisted for only a short period of time (2-4 h). DNA damage checkpoint and non-homologous end joining were involved in the radioadaptive response, exhibiting induction of protruding vulva in worms deficient in these two pathways similar to their controls. Interestingly, the DNA damage checkpoint was not active in the somatic vulval cells, and it was therefore suggested that the DNA damage checkpoint might mediate the radioadaptive response in a cell nonautonomous manner. Here, we show evidence of the occurrence of a radioadaptive response for RCD in the vulval tissue model of C. elegans . This finding provides a potential opportunity to gain further insight into the underlying mechanisms of the radioadaptive response for RCD, in view of the abundant genetic resources of C. elegans .
authors.affiliation: Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences and Key Laboratory of Environmental Toxicology and Pollution Control Technology of Anhui Province, Institute of Technical Biology and Agriculture Engineering, Chinese Academy of Sciences, Hefei 230031, Peoples Republic of China.
authors.forename: Huangqi
authors.initials: H
authors.lastname: Tang
authors.affiliation: Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences and Key Laboratory of Environmental Toxicology and Pollution Control Technology of Anhui Province, Institute of Technical Biology and Agriculture Engineering, Chinese Academy of Sciences, Hefei 230031, Peoples Republic of China.
authors.forename: Liangwen
authors.initials: L
authors.lastname: Chen
authors.affiliation: Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences and Key Laboratory of Environmental Toxicology and Pollution Control Technology of Anhui Province, Institute of Technical Biology and Agriculture Engineering, Chinese Academy of Sciences, Hefei 230031, Peoples Republic of China.
authors.forename: Jialu
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences and Key Laboratory of Environmental Toxicology and Pollution Control Technology of Anhui Province, Institute of Technical Biology and Agriculture Engineering, Chinese Academy of Sciences, Hefei 230031, Peoples Republic of China.
authors.forename: Jue
authors.initials: J
authors.lastname: Shi
authors.affiliation: Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences and Key Laboratory of Environmental Toxicology and Pollution Control Technology of Anhui Province, Institute of Technical Biology and Agriculture Engineering, Chinese Academy of Sciences, Hefei 230031, Peoples Republic of China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Li
authors.affiliation: Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences and Key Laboratory of Environmental Toxicology and Pollution Control Technology of Anhui Province, Institute of Technical Biology and Agriculture Engineering, Chinese Academy of Sciences, Hefei 230031, Peoples Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences and Key Laboratory of Environmental Toxicology and Pollution Control Technology of Anhui Province, Institute of Technical Biology and Agriculture Engineering, Chinese Academy of Sciences, Hefei 230031, Peoples Republic of China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Wu
authors.affiliation: Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences and Key Laboratory of Environmental Toxicology and Pollution Control Technology of Anhui Province, Institute of Technical Biology and Agriculture Engineering, Chinese Academy of Sciences, Hefei 230031, Peoples Republic of China.
authors.forename: Furu
authors.initials: F
authors.lastname: Zhan
authors.affiliation: Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences and Key Laboratory of Environmental Toxicology and Pollution Control Technology of Anhui Province, Institute of Technical Biology and Agriculture Engineering, Chinese Academy of Sciences, Hefei 230031, Peoples Republic of China.
authors.forename: Po
authors.initials: P
authors.lastname: Bian
mesh_terms: D000222:Adaptation, Physiological / Q000528:radiation effects*; D000818:Animals; D017173:Caenorhabditis elegans; D016923:Cell Death / Q000528:radiation effects*; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005720:Gamma Rays; D011836:Radiation Tolerance; D012098:Reproduction; D014844:Vulva / Q000166:cytology* / Q000528:radiation effects*