title: Novel synthetic chalcones induces apoptosis in human glioblastoma cells.
abstract: Glioblastoma multiforme is the main and most frequent tumor in adults' central nervous system. With a survival average of 5% two years after diagnosis, this type of cancer is a main health problem. Substances like the chalcones have been tested in order to develop new treatments. Here, we studied the effects of three synthetic chalcones (A23, C31 and J11) on A172 and surgery obtained-glioma cells. All chalcones showed a decrease in cell viability, mainly C31. An increase in apoptosis levels with no further increase of necrosis was observed. This augmentation may be linked to the high oxidative effect found, caused by the increased presence of reactive oxygen species and nitric oxide production. Cell cycle distribution showed an arrest at G0/G1 and S phases, suggesting that C31 interferes in cell cycle control. Our results shall aid in directing future research with this substance and its antitumor effect.
authors.affiliation: Laboratório de Biologia de Gliomas, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Trindade, CEP: 88040-900, Florianópolis, SC, Brazil.
authors.forename: Lucas Felipe Fernandes
authors.initials: LF
authors.lastname: Bittencourt
authors.affiliation: Laboratório de Biologia de Gliomas, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Trindade, CEP: 88040-900, Florianópolis, SC, Brazil; Laboratório de Neuroquímica-4, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Trindade, CEP: 88040-900, Florianópolis, SC, Brazil.
authors.forename: Karen Andrinéia de
authors.initials: KA
authors.lastname: Oliveira
authors.affiliation: Laboratório de Biologia de Gliomas, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Trindade, CEP: 88040-900, Florianópolis, SC, Brazil.
authors.forename: Carine Bropp
authors.initials: CB
authors.lastname: Cardoso
authors.affiliation: Laboratório de Biologia de Gliomas, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Trindade, CEP: 88040-900, Florianópolis, SC, Brazil.
authors.forename: Flávia Garcia
authors.initials: FG
authors.lastname: Lopes
authors.affiliation: Laboratório de Neuroquímica-4, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Trindade, CEP: 88040-900, Florianópolis, SC, Brazil.
authors.forename: Tharine
authors.initials: T
authors.lastname: Dal-Cim
authors.affiliation: Laboratório Estrutura e Atividade (LEAT), Departamento de Química, Universidade Federal de Santa Catarina, Campus Trindade, CEP: 88040-900, Florianópolis, SC, Brazil.
authors.forename: Louise Domeneghini
authors.initials: LD
authors.lastname: Chiaradia-Delatorre
authors.affiliation: Laboratório Estrutura e Atividade (LEAT), Departamento de Química, Universidade Federal de Santa Catarina, Campus Trindade, CEP: 88040-900, Florianópolis, SC, Brazil.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Mascarello
authors.affiliation: Laboratório de Genética, Divisão de Análises Clinicas, Hospital Universitário, Universidade Federal de Santa Catarina, Campus Trindade, CEP: 88040-900, Florianópolis, SC, Brazil.
authors.forename: Sharbel Weidner
authors.initials: SW
authors.lastname: Maluf
authors.affiliation: Laboratório Estrutura e Atividade (LEAT), Departamento de Química, Universidade Federal de Santa Catarina, Campus Trindade, CEP: 88040-900, Florianópolis, SC, Brazil.
authors.forename: Rosendo Augusto
authors.initials: RA
authors.lastname: Yunes
authors.affiliation: Laboratório de Biologia de Gliomas, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Trindade, CEP: 88040-900, Florianópolis, SC, Brazil.
authors.forename: Ricardo Castilho
authors.initials: RC
authors.lastname: Garcez
authors.affiliation: Laboratório de Neuroquímica-4, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Trindade, CEP: 88040-900, Florianópolis, SC, Brazil.
authors.forename: Carla Inês
authors.initials: CI
authors.lastname: Tasca
authors.affiliation: Laboratório de Biologia de Gliomas, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Trindade, CEP: 88040-900, Florianópolis, SC, Brazil. Electronic address: claudianedel@gmail.com.
authors.forename: Cláudia Beatriz
authors.initials: CB
authors.lastname: Nedel
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D001932:Brain Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D047188:Chalcones / Q000737:chemistry / Q000494:pharmacology*; D005909:Glioblastoma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D047188:Chalcones; D017382:Reactive Oxygen Species
keywords: Cancer; Cell cycle; Cell death; Glioblastoma multiforme; Glioma; Nitric oxide; Oxidative stress; Reactive oxygen species