title: New nasal nanocomplex self-assembled from charged biomacromolecules: N,N,N-Trimethyl chitosan and dextran sulfate.
abstract: Although chitosan (CHT, a linear cationic polysaccharide) is biodegradable, biocompatible, non-toxic, and mucoadhesive in nature, the low solubility of CHT in aqueous and alkaline media limits its applicability in pharmaceutical and biomedical field. This necessitate the introduction of new chemically-modified derivatives of CHT those can surmount the solubility barrier. Herein, N,N,N-trimethyl chitosan (TMC), a quaternized hydrophilic derivative of CHT, was synthesized by two-step reductive methylation of CHT and characterized for (1)H NMR and zeta potential measurements. Polyelectrolyte complexes (PECs) based on TMC and dextran sulfate (DS) were prepared via ionic interactions between charged functional groups of former polysaccharides at different pH conditions (pH 5, 8, 10, and 12) and characterized for physicochemical (particle size and zeta potential) and solid- state characterizations (HR-TEM, SEM, FTIR, TGA and XRD). At alkaline pH conditions, the participant polymer chains (TMC and DS) are sufficiently close to form more stable PECs. The release efficiency was assessed after loading a model drug into optimized PEC formulation. Data indicated that the PECs fabricated at alkaline pH presents a reliable formulation for pharmaceutical and biomedical applications.
authors.affiliation: Industrial Pharmacy Laboratory, Department of Pharmaceutics, R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Maharashtra, India.
authors.forename: Abhijeet D
authors.initials: AD
authors.lastname: Kulkarni
authors.affiliation: Industrial Pharmacy Laboratory, Department of Pharmaceutics, R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Maharashtra, India.
authors.forename: Yogesh H
authors.initials: YH
authors.lastname: Vanjari
authors.affiliation: Industrial Pharmacy Laboratory, Department of Pharmaceutics, R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Maharashtra, India.
authors.forename: Karan H
authors.initials: KH
authors.lastname: Sancheti
authors.affiliation: Department of Pharmaceutical Chemistry, R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Maharashtra, India.
authors.forename: Harun M
authors.initials: HM
authors.lastname: Patel
authors.affiliation: Department of Pharmaceutical Sciences, R.T.M. Nagpur University, Nagpur, Maharashtra, India.
authors.forename: Veena S
authors.initials: VS
authors.lastname: Belgamwar
authors.affiliation: Industrial Pharmacy Laboratory, Department of Pharmaceutics, R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Maharashtra, India.
authors.forename: Sanjay J
authors.initials: SJ
authors.lastname: Surana
authors.affiliation: Industrial Pharmacy Laboratory, Department of Pharmaceutics, R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Maharashtra, India. Electronic address: chandrakantpardeshi11@gmail.com.
authors.forename: Chandrakantsing V
authors.initials: CV
authors.lastname: Pardeshi
mesh_terms: D000281:Administration, Intranasal; D048271:Chitosan / Q000138:chemical synthesis / Q000737:chemistry* / Q000627:therapeutic use; D016264:Dextran Sulfate / Q000737:chemistry* / Q000627:therapeutic use; D004339:Drug Compounding; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D053758:Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use; D010316:Particle Size; D012995:Solubility
chemical_list: C118071:N-trimethyl chitosan chloride; D048271:Chitosan; D016264:Dextran Sulfate
keywords: Chitosan; Dextran sulfate; N,N,N-Trimethyl chitosan; Nasal delivery; Polyelectrolyte nanocomplex; Reductive methylation