title: Deep sequencing leads to the identification of eukaryotic translation initiation factor 5A as a key element in Rsv1-mediated lethal systemic hypersensitive response to Soybean mosaic virus infection in soybean.
abstract: Rsv1, a single dominant resistance locus in soybean, confers extreme resistance to the majority of Soybean mosaic virus (SMV) strains, but is susceptible to the G7 strain. In Rsv1-genotype soybean, G7 infection provokes a lethal systemic hypersensitive response (LSHR), a delayed host defence response. The Rsv1-mediated LSHR signalling pathway remains largely unknown. In this study, we employed a genome-wide investigation to gain an insight into the molecular interplay between SMV G7 and Rsv1-genotype soybean. Small RNA (sRNA), degradome and transcriptome sequencing analyses were used to identify differentially expressed genes (DEGs) and microRNAs (DEMs) in response to G7 infection. A number of DEGs, DEMs and microRNA targets, and the interaction network of DEMs and their target mRNAs responsive to G7 infection, were identified. Knock-down of one of the identified DEGs, the eukaryotic translation initiation factor 5A (eIF5A), diminished the LSHR and enhanced viral accumulation, suggesting the essential role of eIF5A in the G7-induced, Rsv1-mediated LSHR signalling pathway. This work provides an in-depth genome-wide analysis of high-throughput sequencing data, and identifies multiple genes and microRNA signatures that are associated with the Rsv1-mediated LSHR.
authors.affiliation: London Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON, Canada, N5T 4T3.|Department of Biology, University of Western Ontario, London, ON, Canada, N6A 5B7.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.affiliation: London Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON, Canada, N5T 4T3.
authors.forename: Andrej
authors.initials: A
authors.lastname: Adam Arsovski
authors.affiliation: Greenhouse and Processing Crops Research Centre, Agriculture and Agri-Food Canada, Harrow, ON, Canada, N0R 1G0.
authors.forename: Kangfu
authors.initials: K
authors.lastname: Yu
authors.affiliation: London Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON, Canada, N5T 4T3.|Department of Biology, University of Western Ontario, London, ON, Canada, N6A 5B7.
authors.forename: Aiming
authors.initials: A
authors.lastname: Wang
mesh_terms: D030541:Databases, Genetic; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D017343:Genes, Plant; D005838:Genotype; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D010448:Peptide Initiation Factors / Q000235:genetics* / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology* / Q000821:virology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D017800:Potyvirus / Q000502:physiology*; D020871:RNA Stability / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D013025:Glycine max / Q000235:genetics / Q000276:immunology* / Q000821:virology*; D014158:Transcription, Genetic; D000097574:Eukaryotic Translation Initiation Factor 5A
chemical_list: D035683:MicroRNAs; D010448:Peptide Initiation Factors; D010940:Plant Proteins; D016601:RNA-Binding Proteins; D000097574:Eukaryotic Translation Initiation Factor 5A
keywords: Soybean mosaic virus; degradome-seq; eukaryotic translation initiation factor; lethal systemic hypersensitive response; sRNA-seq; transcriptome-seq; virus-host interaction