title: Grinding and polishing instead of sectioning for the tissue samples with a graft: Implications for light and electron microscopy.
abstract: A broad use of the graft replacement requires a detailed investigation of the host-graft interaction, including both histological examination and electron microscopy. A high quality sectioning of the host tissue with a graft seems to be complicated; in addition, it is difficult to examine the same tissue area by both of the mentioned microscopy techniques. To solve these problems, we developed a new technique of epoxy resin embedding with the further grinding, polishing, and staining. Graft-containing tissues prepared by grinding and polishing preserved their structure; however, sectioning frequently required the explantation of the graft and led to tissue disintegration. Moreover, stained samples prepared by grinding and polishing may then be assessed by both light microscopy and backscattered scanning electron microscopy. Therefore, grinding and polishing outperform sectioning when applied to the tissues with a graft.
authors.affiliation: Division of Experimental and Clinical Cardiology, Research Institute for Complex Issues of Cardiovascular Diseases, Sosnovy Boulevard 6, 650002 Kemerovo, Russian Federation.
authors.forename: Rinat A
authors.initials: RA
authors.lastname: Mukhamadiyarov
authors.affiliation: Division of Experimental and Clinical Cardiology, Research Institute for Complex Issues of Cardiovascular Diseases, Sosnovy Boulevard 6, 650002 Kemerovo, Russian Federation.
authors.forename: Victoria V
authors.initials: VV
authors.lastname: Sevostyanova
authors.affiliation: Division of Experimental and Clinical Cardiology, Research Institute for Complex Issues of Cardiovascular Diseases, Sosnovy Boulevard 6, 650002 Kemerovo, Russian Federation.
authors.forename: Daria K
authors.initials: DK
authors.lastname: Shishkova
authors.affiliation: Division of Experimental and Clinical Cardiology, Research Institute for Complex Issues of Cardiovascular Diseases, Sosnovy Boulevard 6, 650002 Kemerovo, Russian Federation.
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Nokhrin
authors.affiliation: Department of Pathology, Kemerovo State Medical Academy, Voroshilova Street 22a, 650029 Kemerovo, Russian Federation.
authors.forename: Olga D
authors.initials: OD
authors.lastname: Sidorova
authors.affiliation: Division of Experimental and Clinical Cardiology, Research Institute for Complex Issues of Cardiovascular Diseases, Sosnovy Boulevard 6, 650002 Kemerovo, Russian Federation. Electronic address: antonkutikhin@gmail.com.
authors.forename: Anton G
authors.initials: AG
authors.lastname: Kutikhin
mesh_terms: D004853:Epoxy Resins; D008853:Microscopy / Q000379:methods*; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D008867:Microtomy / Q000379:methods; D013048:Specimen Handling / Q000379:methods*; D013499:Surface Properties; D016610:Tissue Embedding / Q000379:methods
chemical_list: D004853:Epoxy Resins
keywords: Electron microscopy; Graft; Grinding; Light microscopy; Polishing; Sample preparation