title: Changes in the Immune System of Female Wistar Rats After Exposure to Immunosuppressive Treatment During Pregnancy.
abstract: This experimental study assessed the impact of medications frequently used after kidney transplantation on the immune system of pregnant female Wistar rats. The study evaluates medications, both approved and contraindicated during pregnancy in common therapeutic combinations. The study was conducted on 32 female Wistar rats, subjected to immunosuppressive regimens most commonly used in therapy of human kidney transplant recipients (cyclosporine A, mycophenolate mofetil and prednisone; tacrolimus, mycophenolate mofetil and prednisone; and cyclosporine A, everolimus and prednisone). The animals received drugs by oral gavage 2 weeks before pregnancy and at 3 weeks of pregnancy. We found drug regimen-dependent differences in cytometry from spleen. Many subpopulations of lymphocytes were suppressed in rats treated with cyclosporine A, mycophenolate mofetil and prednisone and tacrolimus, mycophenolate mofetil and prednisone; the number of NK cells was increased in group of rats treated with cyclosporine A, everolimus and prednisone. We also found changes in histological examination of thymus and spleen of all treated dams. In cytokine assay, we noticed increasing levels of IL-17 with increasing doses of concanavalin A in control group and in group of dams treated with cyclosporine A, mycophenolate mofetil and prednisone. This increase was blocked in rats treated with tacrolimus, mycophenolate mofetil and prednisone and cyclosporine A, everolimus and prednisone. Qualitative, quantitative and morphological changes of immune system in pharmacologically immunosuppressed females have been observed. Thymus structure, spleen composition and splenocytes IL-17 production were mostly affected in drug regimen-dependent manner.
authors.affiliation: Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczecin, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Kabat-Koperska
authors.affiliation: Department of Histology and Embryology, Pomeranian Medical University, Szczecin, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Kolasa-Wołosiuk
authors.affiliation: Department of Microbiology and Immunological Diagnostics, Pomeranian Medical University, Szczecin, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Wojciuk
authors.affiliation: Department of Microbiology and Immunological Diagnostics, Pomeranian Medical University, Szczecin, Poland.
authors.forename: I
authors.initials: I
authors.lastname: Wojciechowska-Koszko
authors.affiliation: Department of Microbiology and Immunological Diagnostics, Pomeranian Medical University, Szczecin, Poland.
authors.forename: P
authors.initials: P
authors.lastname: Roszkowska
authors.affiliation: Department of Microbiology and Immunological Diagnostics, Pomeranian Medical University, Szczecin, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Krasnodębska-Szponder
authors.affiliation: Department of General Pathology, Pomeranian Medical University, Szczecin, Poland.
authors.forename: E
authors.initials: E
authors.lastname: Paczkowska
authors.affiliation: Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Szczecin, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Safranow
authors.affiliation: Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczecin, Poland.
authors.forename: E
authors.initials: E
authors.lastname: Gołembiewska
authors.affiliation: Department of General Pathology, Pomeranian Medical University, Szczecin, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Machaliński
authors.affiliation: Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczecin, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Ciechanowski
mesh_terms: D000284:Administration, Oral; D000818:Animals; D005260:Female; D006084:Graft Rejection / Q000517:prevention & control*; D006801:Humans; D007165:Immunosuppression Therapy; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D020381:Interleukin-17 / Q000378:metabolism; D016030:Kidney Transplantation; D007694:Killer Cells, Natural / Q000276:immunology*; D011247:Pregnancy / Q000187:drug effects* / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000187:drug effects* / Q000276:immunology; D013950:Thymus Gland / Q000187:drug effects* / Q000276:immunology
chemical_list: D007166:Immunosuppressive Agents; D020381:Interleukin-17