title: A Biological Approach for the Synthesis of Bismuth Nanoparticles: Using Thiolated M13 Phage as Scaffold.
abstract: We report the synthesis of Bi nanoparticles (Bi NPs) using the M13 phage as scaffold. The p8 protein of the phage is functionalized with thiol groups of different lengths, and these thiolated regions act as nucleation centers for Bi(3+) ions. The size distribution, shape, and resilience to oxidation of the Bi NPs depend on the length of the thiol group used. The NPs are characterized by high resolution transmission electron microscopy, Raman, and IR spectroscopies, matrix assisted laser desorption/ionization, and optical absorption. These results show that the nanoparticles are crystalline and have a typical diameter of ∼3.0 nm. The method of preparation presented here is reproducible and implies "greener" conditions than those reported elsewhere. To the best of our knowledge, this is the first report of bismuth nanoparticles synthesized by a biomineralization method.
authors.affiliation: Departamento de Química, Área de Biofisicoquímica, Universidad Autónoma Metropolitana-Iztapalapa , San Rafael Atlixco No 186, Col. Vicentina, 09340, México D.F., México.
authors.forename: L Irais
authors.initials: LI
authors.lastname: Vera-Robles
authors.affiliation: Departamento de Física, Instituto Nacional de Investigaciones Nucleares , Apdo Postal 18-1027, México D.F., México.
authors.forename: Luis
authors.initials: L
authors.lastname: Escobar-Alarcón
authors.affiliation: Departamento de Física, Universidad Autónoma Metropolitana-Iztapalapa , San Rafael Atlixco No.186, Col. Vicentina, 09340 México D.F., México.
authors.forename: Michel
authors.initials: M
authors.lastname: Picquart
authors.affiliation: Departamento de Física, Universidad Autónoma Metropolitana-Iztapalapa , San Rafael Atlixco No.186, Col. Vicentina, 09340 México D.F., México.
authors.forename: J Luis
authors.initials: JL
authors.lastname: Hernández-Pozos
authors.affiliation: Departamento de Física, Universidad Autónoma Metropolitana-Iztapalapa , San Rafael Atlixco No.186, Col. Vicentina, 09340 México D.F., México.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Haro-Poniatowski
mesh_terms: D017104:Bacteriophage M13 / Q000737:chemistry*; D040761:Biomimetic Materials; D001729:Bismuth / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D011422:Propionates / Q000737:chemistry; D013059:Spectrum Analysis, Raman; D013388:Succinimides / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013440:Sulfides / Q000737:chemistry; D014764:Viral Proteins / Q000737:chemistry*
chemical_list: C000613816:N-succinimidyl S-acetylthiopropionate; D011422:Propionates; D013388:Succinimides; D013438:Sulfhydryl Compounds; D013440:Sulfides; D014764:Viral Proteins; C039011:N-hydroxysuccinimide S-acetylthioacetate; D001729:Bismuth