title: Wavelength-resolved simultaneous photoelectrochemical bifunctional sensor on single interface: A newly in vitro approach for multiplexed DNA monitoring in cancer cells.
abstract: Currently, the photoelectrochemical (PEC) strategies can just achieve single analyte detection on a single interface with limited detection efficiency. It is highly valuable but full of challenge to develop a PEC biosensor for multiple analytes evaluation on a single interface. For this point, the wavelength-selective photoactive materials, which could generate separated photocurrents under excitation lights with certain wavelengths, were mainly important to overcome this challenge. Herein, these wavelength-selective photoactive materials were successfully synthesized and served as signal indicators to construct a novel PEC biosensor for multiple analytes evaluation on a single interface for the first time. Moreover, an enzyme-assisted target recycling amplification strategy was introduced for ultrasensitive monitoring. As a result, the proposed PEC biosensor showed excellent analytical performance for both oral cancer (ORVOA 1) gene and p53 gene down to attomolar level. In addition, the fabricated PEC biosensor was employed to evaluate ORVOA 1 gene and p53 gene in Hela cells. This assay has laid the foundation for fabrication of simple, ultrasensitive and economical PEC diagnostic devices to detect multiple analytes in cells, which paved a new avenue for early diagnosis of cancer with higher efficiency and accuracy.
authors.affiliation: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.
authors.forename: Yingning
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China; Department of Clinical Biochemistry, Laboratory Sciences, Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, People's Republic of China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Liang
authors.affiliation: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.
authors.forename: Chengyi
authors.initials: C
authors.lastname: Xiong
authors.affiliation: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.
authors.forename: Shunbi
authors.initials: S
authors.lastname: Xie
authors.affiliation: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.
authors.forename: Haijun
authors.initials: H
authors.lastname: Wang
authors.affiliation: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China. Electronic address: yaqinchai@swu.edu.cn.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Chai
authors.affiliation: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China. Electronic address: yuanruo@swu.edu.cn.
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis / Q000235:genetics*; D055664:Electrochemical Techniques / Q000379:methods*; D016158:Genes, p53; D006367:HeLa Cells; D006801:Humans; D057230:Limit of Detection; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D009363:Neoplasm Proteins / Q000235:genetics; D009369:Neoplasms / Q000235:genetics*; D055668:Photochemical Processes
chemical_list: C465628:LTO1 protein, human; D009363:Neoplasm Proteins; D004247:DNA
keywords: Cell; Multiplexed detection; Photoelectrochemical biosensor; Signal amplification; Single interface