title: Factors associated with success of image-guided tumour biopsies: Results from a prospective molecular triage study (MOSCATO-01).
abstract: MOSCATO-01 is a molecular triage trial based on on-purpose tumour biopsies to perform molecular portraits. We aimed at identifying factors associated with high tumour cellularity.
  
  
  
  Tumour cellularity (percentage of tumour cells in samples defined at pathology) was evaluated according to patient characteristics, target lesion characteristics, operators' experience and biopsy approach.
  
  
  
  Among 460 patients enrolled between November, 2011 and March, 2014, 334 patients (73%) had an image-guided needle biopsy of the primary tumour (N = 38) or a metastatic lesion (N = 296). Biopsies were performed on liver (N = 127), lung (N = 72), lymph nodes (N = 71), bone (N = 11), or another tumour site (N = 53). Eighteen patients (5%) experienced a complication: pneumothorax in 10 patients treated medically, and haemorrhage in 8, requiring embolisation in 3 cases. Median tumour cellularity was 50% (interquartile range, 30-70%). The molecular analysis was successful in 291/334 cases (87%). On-going chemotherapy, tumour origin (primary versus metastatic), lesion size, tumour growth rate, presence of necrosis on imaging, standardised uptake value, and needle size were not statistically associated with cellularity. Compared to liver or lung biopsies, cellularity was significantly lower in bone and higher in other sites (P < 0.0001). Cellularity significantly increased with the number of collected samples (P < 0.0001) and was higher in contrast-enhanced ultrasound-guided biopsies (P < 0.02). In paired samples, cellularity in central samples was lower than in peripheral samples in 85, equal in 68 and higher in 89 of the cases.
  
  
  
  Image-guided biopsy is feasible and safe in cancer patients for molecular screening. Imaging modality, multiple sampling of the lesion, and the organ chosen for biopsy were associated with higher tumour cellularity.
authors.affiliation: Gustave Roussy, Département de Radiologie interventionnelle, France. Electronic address: vaniatacher@gmail.com.
authors.forename: Vania
authors.initials: V
authors.lastname: Tacher
authors.affiliation: INSERM, CESP Equipe 2, France; Gustave Roussy, Service de Biostatistique et d'Epidémiologie, Université Paris-Sud, UFR de Médecine, France. Electronic address: Marie-Cecile.LEDELEY@gustaveroussy.fr.
authors.forename: Marie-Cécile
authors.initials: MC
authors.lastname: Le Deley
authors.affiliation: Gustave Roussy, Département d'Innovation Thérapeutique et d'Essais Précoces, France. Electronic address: Antoine.HOLLEBECQUE@gustaveroussy.fr.
authors.forename: Antoine
authors.initials: A
authors.lastname: Hollebecque
authors.affiliation: Gustave Roussy, Département de Radiologie interventionnelle, France. Electronic address: Frederic.DESCHAMPS@gustaveroussy.fr.
authors.forename: Frederic
authors.initials: F
authors.lastname: Deschamps
authors.affiliation: Gustave Roussy, Département d'Anatomopathologie, France. Electronic address: Philippe.VIELH@gustaveroussy.fr.
authors.forename: Philippe
authors.initials: P
authors.lastname: Vielh
authors.affiliation: Gustave Roussy, Département de Radiologie interventionnelle, France. Electronic address: thakime@yahoo.com.
authors.forename: Antoine
authors.initials: A
authors.lastname: Hakime
authors.affiliation: Gustave Roussy, Département d'Innovation Thérapeutique et d'Essais Précoces, France. Electronic address: Elena.ILEANAECATERINA@gustaveroussy.fr.
authors.forename: Ecaterina
authors.initials: E
authors.lastname: Ileana
authors.affiliation: Gustave Roussy, Département d'Anatomopathologie, France. Electronic address: BEHNOUSH.ABEDI@gustaveroussy.fr.
authors.forename: Behnoush
authors.initials: B
authors.lastname: Abedi-Ardekani
authors.affiliation: Gustave Roussy, Département d'Anatomopathologie, France. Electronic address: Cecile.CHARPY@gustaveroussy.fr.
authors.forename: Cécile
authors.initials: C
authors.lastname: Charpy
authors.affiliation: Gustave Roussy, Département d'Innovation Thérapeutique et d'Essais Précoces, France. Electronic address: Christophe.MASSARD@gustaveroussy.fr.
authors.forename: Christophe
authors.initials: C
authors.lastname: Massard
authors.affiliation: Gustave Roussy, Service de Biostatistique et d'Epidémiologie, Université Paris-Sud, UFR de Médecine, France. Electronic address: SILVIA.ROSELLINI@gustaveroussy.fr.
authors.forename: Silvia
authors.initials: S
authors.lastname: Rosellini
authors.affiliation: Gustave Roussy, Service de Biostatistique et d'Epidémiologie, Université Paris-Sud, UFR de Médecine, France. Electronic address: dorota_gajda@yahoo.fr.
authors.forename: Dorota
authors.initials: D
authors.lastname: Gajda
authors.affiliation: Gustave Roussy, Service de Biostatistique et d'Epidémiologie, Université Paris-Sud, UFR de Médecine, France. Electronic address: Aljosa.CELEBIC@gustaveroussy.fr.
authors.forename: Aljosa
authors.initials: A
authors.lastname: Celebic
authors.affiliation: Gustave Roussy, Département of Medical Oncology, France. Electronic address: Charles.FERTE@gustaveroussy.fr.
authors.forename: Charles
authors.initials: C
authors.lastname: Ferté
authors.affiliation: Gustave Roussy, Département d'Innovation Thérapeutique et d'Essais Précoces, France. Electronic address: Maud.NGOCAMUS@gustaveroussy.fr.
authors.forename: Maud
authors.initials: M
authors.lastname: Ngo-Camus
authors.affiliation: Gustave Roussy, Service de Biostatistique et d'Epidémiologie, Université Paris-Sud, UFR de Médecine, France. Electronic address: SIHAM.GOUISSEM@gustaveroussy.fr.
authors.forename: Siham
authors.initials: S
authors.lastname: Gouissem
authors.affiliation: Gustave Roussy, Laboratoire de Recherche Translationnelle, France. Electronic address: Valerie.KOUBI-PICK@gustaveroussy.fr.
authors.forename: Valérie
authors.initials: V
authors.lastname: Koubi-Pick
authors.affiliation: Gustave Roussy, Département of Medical Oncology, France; Université Paris-Sud, UFR Médicale Le Kremlin-Bicêtre, France. Electronic address: Fabrice.ANDRE@gustaveroussy.fr.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Andre
authors.affiliation: Université Paris-Sud, UFR Médicale Le Kremlin-Bicêtre, France; Gustave Roussy, Direction de la Recherche Clinique, France. Electronic address: Gilles.Vassal@gustaveroussy.fr.
authors.forename: Gilles
authors.initials: G
authors.lastname: Vassal
authors.affiliation: Gustave Roussy, Département de Médecine Nucléaire, France. Electronic address: Desiree.DEANDREIS@gustaveroussy.fr.
authors.forename: Désirée
authors.initials: D
authors.lastname: Deandreis
authors.affiliation: Gustave Roussy, Laboratoire de Recherche Translationnelle, France; Université Paris-Sud, UFR Médicale Le Kremlin-Bicêtre, France. Electronic address: Ludovic.LACROIX@gustaveroussy.fr.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Lacroix
authors.affiliation: Gustave Roussy, Département d'Innovation Thérapeutique et d'Essais Précoces, France; Université Paris-Sud, UFR Médicale Le Kremlin-Bicêtre, France. Electronic address: Jean-Charles.Soria@gustaveroussy.fr.
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Soria
authors.affiliation: Gustave Roussy, Département de Radiologie interventionnelle, France; Université Paris-Sud, UFR Médicale Le Kremlin-Bicêtre, France. Electronic address: Thierry.DEBAERE@gustaveroussy.fr.
authors.forename: Thierry
authors.initials: T
authors.lastname: De Baère
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D061705:Image-Guided Biopsy / Q000009:adverse effects / Q000379:methods; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009369:Neoplasms / Q000473:pathology* / Q000628:therapy; D011446:Prospective Studies; D014218:Triage; D055815:Young Adult
keywords: Image-guided tumour biopsies; Molecular triage