title: Preparation of nanoliposome loaded with peanut peptide fraction: stability and bioavailability.
abstract: Nanoliposome loaded with peanut peptide fraction (PPF) prepared by high pressure microfluidization (HPM) treatment was investigated as well as its stability and bioavailability. PPF showed hydrophilicity character with a solubility of 97.50 ± 2.31 mg mL(-1) in aqueous solution. HPM treatment can prepare nanoliposome but decreased encapsulation efficiency (EE). A pressure of 120 MPa was the appropriate parameter where the particle size and EE of nanolipsome was 79.67 ± 1.85 nm and 65.12 ± 2.96%, respectively. Crude liposome and nanoliposome both showed good stability under different pH conditions, even at pH value of 2.0. Nanoliposome behaved better in vitro controlled release than crude liposome. Most important of all, nanoliposome had the highest angiotension converting enzyme (ACE) inhibitory activity after simulated gastrointestinal tract (GIT) digestion. Morphology of digested liposome proved that nanoliposome can keep relative integrity in structure although it suffered a lot of attack.
authors.affiliation: Institute of Agro-products Processing and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.O. Box 5109, Beijing 100193, China. wangqiang06@caas.cn and Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
authors.forename: Kui-Jie
authors.initials: KJ
authors.lastname: Gong
authors.affiliation: Institute of Agro-products Processing and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.O. Box 5109, Beijing 100193, China. wangqiang06@caas.cn.
authors.forename: Ai-Min
authors.initials: AM
authors.lastname: Shi
authors.affiliation: Institute of Agro-products Processing and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.O. Box 5109, Beijing 100193, China. wangqiang06@caas.cn.
authors.forename: Hong-Zhi
authors.initials: HZ
authors.lastname: Liu
authors.affiliation: Institute of Agro-products Processing and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.O. Box 5109, Beijing 100193, China. wangqiang06@caas.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Institute of Agro-products Processing and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.O. Box 5109, Beijing 100193, China. wangqiang06@caas.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Hu
authors.affiliation: Institute of Agro-products Processing and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.O. Box 5109, Beijing 100193, China. wangqiang06@caas.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.affiliation: School of Applied Sciences, RMIT University, City Campus, Melbourne, VIC 3001, Australia.
authors.forename: Benu
authors.initials: B
authors.lastname: Adhikari
authors.affiliation: Institute of Agro-products Processing and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.O. Box 5109, Beijing 100193, China. wangqiang06@caas.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
mesh_terms: D010367:Arachis / Q000737:chemistry* / Q000378:metabolism; D004063:Digestion; D004339:Drug Compounding / Q000379:methods*; D004355:Drug Stability; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism; D010316:Particle Size; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D012995:Solubility
chemical_list: D008081:Liposomes; D010455:Peptides