title: [Histone acetylation modification of topoisomerase enzyme Ⅱα promoter regulation factors in patients with chronic benzene poisoning].
abstract: OBJECTIVE
  To investigate histone acetylation modification of topoisomerase enzyme Ⅱα (TOPOⅡα) promoter regulation factors in patients with chronic benzene poisoning, to explore the possible regulatory mechanism of TOPOⅡα involved in toxicity of chronic benzene poisoning;
  
  
  METHODS
  The bone marrow samples were from 25 chronic benzene poisoning cases and 25 controls. The Chromatin Immunoprecipitation (ChIP) assay was carried out to study the possible mechanism of TOPOⅡα promoter regulation factors expression changes. TOPOⅡα promoter regulation factors mRNA were detected by RT-PCR technique.
  
  
  RESULTS
  (1) Compared with the control, the histone H4 acetylation, histone H3 acetylation level of TOPOⅡα promoter regulation factors SP1, ATF-2, SP3, NF-YA, P53, C-MYB, ICBP90, NF-M in chronic benzene poisoning patients decreased, with the significant difference (P<0.05) , except for C-JUN (P>0.05) ; (2) The mRNA expression of TOPOⅡαpromoter regulation factors SP1, NF-YA, C-MYB, C-JUN and NF-M were significantly lower than in the control with the significant difference (P<0.05) , while the expression of SP3、P53 mRNA increased (P<0.05) , ATF-2、ICBP90 mRNA wasn't changed (P>0.05) .
  
  
  CONCLUSIONS
  (1) Chronic benzene poisoning TOPO Ⅱα promoter regulation factors histone modification changes accompanied with mRNA level changed. (2) Histone acetylation modification of topoisomerase enzyme Ⅱα promoter regulation factors takes important role in the benezen's Hematopoietic toxicity.
authors.affiliation: Department of Hematology, the first affiliated hospital of Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Yifen
authors.initials: Y
authors.lastname: Shi
authors.forename: Shanhu
authors.initials: S
authors.lastname: Qian
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Li
authors.forename: Kang
authors.initials: K
authors.lastname: Yu
mesh_terms: D000107:Acetylation; D000951:Antigens, Neoplasm / Q000378:metabolism*; D001554:Benzene / Q000506:poisoning*; D016022:Case-Control Studies; D047369:Chromatin Immunoprecipitation; D002908:Chronic Disease; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D006801:Humans; D011041:Poisoning / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D000951:Antigens, Neoplasm; D004268:DNA-Binding Proteins; D006657:Histones; D012333:RNA, Messenger; D004250:DNA Topoisomerases, Type II; D001554:Benzene