title: Immunoexpression of tumour necrosis factor-α, interleukin-1α and interleukin-10 on odontogenic cysts and tumours.
abstract: OBJECTIVE
  To analyse the immunoreactivity of IL-1α, TNF-α and IL-10 in odontogenic cysts and tumours and to investigate possible associations with established biological behaviours of these different lesions.
  
  
  METHODS
  Immunohistochemical expression of anti-IL-1α, anti-TNF-α and anti-IL-10 antibodies was assessed on epithelium and mesenchyme of 20 radicular cysts (RCs), 20 residual cysts (RECs), 20 dentigerous cysts (DCs), 18 solid ameloblastomas (SAs), 20 keratocystic odontogenic tumours (KCOTs) and 15 dental follicles (DFs). Comparative analysis of data was performed using the nonparametric Wilcoxon signed-rank test and Kruskal-Wallis's test.
  
  
  RESULTS
  Significantly greater expression of IL-1α in the epithelium was noted in RC, KCOT and SA (P = 0.01), whilst IL-10 and TNF-α was in the epithelium of RC, DC and KCOT (P < 0.01). In the mesenchyme, significantly greater immunopositivity was observed for IL-1α, IL-10 and TNF-α in KCOT, DC and RC (P < 0.01). In epithelial and mesenchymal tissues, there were a significant number of cases of RC and DC with IL-1α < IL-10 ratio (P < 0.01), whilst SA and KCOT showed IL-1α > IL-10 (P < 0.01). There was a significantly greater percentage of DF, DC and KCOT with TNF-α > IL10 ratio (P < 0.01).
  
  
  CONCLUSIONS
  These results suggest involvement of the proteins in the pathogenesis of odontogenic cysts and tumours, with emphasis on the highest immunoreactivity of osteolysis stimulating factors in tumours with aggressive biological behaviour, such as SA and KCOT.
authors.affiliation: Faculty of Dentistry, Department of Oral Pathology, Federal University of Rio Grande do Norte, Natal, Brazil.
authors.forename: M C
authors.initials: MC
authors.lastname: Sá
authors.affiliation: Department of Dentistry of Lagarto, Federal University of Sergipe, Lagarto, Brazil.
authors.forename: F R
authors.initials: FR
authors.lastname: de Matos
authors.affiliation: Faculty of Dentistry, Department of Oral Pathology, Federal University of Rio Grande do Norte, Natal, Brazil.
authors.forename: T S
authors.initials: TS
authors.lastname: Conceição
authors.affiliation: Department of Oral Pathology, Faculty of Dentistry, Federal University of Bahia, Salvador, Brazil.
authors.forename: A C G H
authors.initials: AC
authors.lastname: Leitão
authors.affiliation: Faculty of Dentistry, Department of Oral Pathology, Federal University of Rio Grande do Norte, Natal, Brazil.
authors.forename: R A
authors.initials: RA
authors.lastname: Freitas
mesh_terms: D003795:Dental Sac / Q000276:immunology / Q000473:pathology; D004848:Epithelium / Q000276:immunology / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D008648:Mesoderm / Q000276:immunology / Q000473:pathology; D009807:Odontogenic Cysts / Q000276:immunology* / Q000473:pathology; D009808:Odontogenic Tumors / Q000276:immunology* / Q000473:pathology; D011842:Radicular Cyst / Q000276:immunology* / Q000473:pathology; D014092:Tooth Root / Q000276:immunology / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha
chemical_list: D014409:Tumor Necrosis Factor-alpha
keywords: interleukin-10; interleukin-1α; odontogenic cysts; odontogenic tumours; tumour necrosis factor-α