title: Genome sequence and plasmid transformation of the model high-yield bacterial cellulose producer Gluconacetobacter hansenii ATCC 53582.
abstract: Bacterial cellulose is a strong, highly pure form of cellulose that is used in a range of applications in industry, consumer goods and medicine. Gluconacetobacter hansenii ATCC 53582 is one of the highest reported bacterial cellulose producing strains and has been used as a model organism in numerous studies of bacterial cellulose production and studies aiming to increased cellulose productivity. Here we present a high-quality draft genome sequence for G. hansenii ATCC 53582 and find that in addition to the previously described cellulose synthase operon, ATCC 53582 contains two additional cellulose synthase operons and several previously undescribed genes associated with cellulose production. In parallel, we also develop optimized protocols and identify plasmid backbones suitable for transformation of ATCC 53582, albeit with low efficiencies. Together, these results provide important information for further studies into cellulose synthesis and for future studies aiming to genetically engineer G. hansenii ATCC 53582 for increased cellulose productivity.
authors.affiliation: Department of Life Sciences, Imperial College London, London, UK.|Centre for Synthetic Biology and Innovation, Imperial College London, London, UK.
authors.forename: Michael
authors.initials: M
authors.lastname: Florea
authors.affiliation: Centre for Synthetic Biology and Innovation, Imperial College London, London, UK.|Department of Bioengineering, Imperial College London, London, UK.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Reeve
authors.affiliation: Bioinformatics Support Service, Department of Surgery and Cancer, Imperial College London, London, UK.|Centre for Integrative Systems Biology and Bioinformatics, Imperial College London, London, UK.
authors.forename: James
authors.initials: J
authors.lastname: Abbott
authors.affiliation: Centre for Synthetic Biology and Innovation, Imperial College London, London, UK.|Department of Medicine, Imperial College London, London, UK.
authors.forename: Paul S
authors.initials: PS
authors.lastname: Freemont
authors.affiliation: Centre for Synthetic Biology and Innovation, Imperial College London, London, UK.|Department of Bioengineering, Imperial College London, London, UK.
authors.forename: Tom
authors.initials: T
authors.lastname: Ellis
mesh_terms: D002482:Cellulose / Q000096:biosynthesis*; D016680:Genome, Bacterial; D041821:Gluconacetobacter / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D010802:Phylogeny; D010957:Plasmids; D014169:Transformation, Bacterial
chemical_list: D002482:Cellulose