title: NADPH oxidases in the arbuscular mycorrhizal symbiosis.
abstract: Plant NADPH oxidases are the major source of reactive oxygen species (ROS) that plays key roles as both signal and stressor in several plant processes, including defense responses against pathogens. ROS accumulation in root cells during arbuscular mycorrhiza (AM) development has raised the interest in understanding how ROS-mediated defense programs are modulated during the establishment of this mutualistic interaction. We have recently analyzed the expression pattern of 5 NADPH oxidase (also called RBOH) encoding genes in Medicago truncatula, showing that only one of them (MtRbohE) is specifically upregulated in arbuscule-containing cells. In line with this result, RNAi silencing of MtRbohE generated a strong alteration in root colonization, with a significant reduction in the number of arbusculated cells. On this basis, we propose that MtRBOHE-mediated ROS production plays a crucial role in the intracellular accommodation of arbuscules.
authors.affiliation: a Dipartimento di Scienze della Vita e Biologia dei Sistemi , Università degli Studi di Torino , Via Accademia Albertina 13, Torino , Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Belmondo
authors.affiliation: a Dipartimento di Scienze della Vita e Biologia dei Sistemi , Università degli Studi di Torino , Via Accademia Albertina 13, Torino , Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Calcagno
authors.affiliation: a Dipartimento di Scienze della Vita e Biologia dei Sistemi , Università degli Studi di Torino , Via Accademia Albertina 13, Torino , Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Genre
authors.affiliation: b INRA, Université de Nice Sophia Antipolis, CNRS, UMR 1355-7254 Institut Sophia Agrobiotech , Sophia Antipolis , France.
authors.forename: Alain
authors.initials: A
authors.lastname: Puppo
authors.affiliation: b INRA, Université de Nice Sophia Antipolis, CNRS, UMR 1355-7254 Institut Sophia Agrobiotech , Sophia Antipolis , France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Pauly
authors.affiliation: a Dipartimento di Scienze della Vita e Biologia dei Sistemi , Università degli Studi di Torino , Via Accademia Albertina 13, Torino , Italy.
authors.forename: Luisa
authors.initials: L
authors.lastname: Lanfranco
mesh_terms: D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D046913:Medicago truncatula / Q000201:enzymology* / Q000235:genetics / Q000382:microbiology*; D038821:Mycorrhizae / Q000502:physiology*; D019255:NADPH Oxidases / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D013559:Symbiosis
chemical_list: D010940:Plant Proteins; D019255:NADPH Oxidases
keywords: Arbuscular mycorrhizal symbiosis; Medicago truncatula; NADPH oxidase; reactive oxygen species (ROS); respiratory burst oxidase homolog (RBOH); spatio-temporal gene expression