title: Functional role of eukaryotic translation initiation factor 4 gamma 1 (EIF4G1) in NSCLC.
abstract: Eukaryotic translation initiation factor 4 gamma 1(EIF4G1) is related to tumorigenesis and tumor progression. However, its role and the underlying mechanisms in the regulation of tumor development in non-small cell lung cancers (NSCLC) remain largely unknown. Here we report that the levels of EIF4G1 expression are much higher in NSCLC cell lines and tumor tissues than those in the normal lung cells and adjacent normal tissues from the same patients. Using shRNA to knock down EIF4G1 expression stably, we found EIF4G1 required for NSCLC cell proliferation, anchorage-independent growth, migration and invasion. Furthermore, silencing of EIF4G1 induces NSCLC cell apoptosis and causes G0/G1 cell cycle arrest. To identify the partner protein network of EIF4G1 in NSCLC cells, we found that Ubiquitin-specific protease 10 (USP10) can directly interacts with EIF4G1, while acting as a negative regulator for EIF4G1-mediated functions. Together, our results indicate that EIF4G1 functions as an oncoprotein during NSCLC development, which may represent a novel and promising therapeutic target in lung cancer.
authors.affiliation: Department of Oncology, Shanghai East Hospital, Dalian Medical University, Shanghai 200120, China.|Research Center for Translational Medicine and Key Laboratory of Arrhythmias, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China.
authors.forename: Yueyu
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Oncology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China.|Research Center for Translational Medicine and Key Laboratory of Arrhythmias, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China.
authors.forename: Mengdan
authors.initials: M
authors.lastname: Wei
authors.affiliation: Department of Oncology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China.|Research Center for Translational Medicine and Key Laboratory of Arrhythmias, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Oncology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China.|Research Center for Translational Medicine and Key Laboratory of Arrhythmias, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Oncology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China.|Research Center for Translational Medicine and Key Laboratory of Arrhythmias, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Oncology, Shanghai East Hospital, Dalian Medical University, Shanghai 200120, China.|Research Center for Translational Medicine and Key Laboratory of Arrhythmias, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Tang
authors.affiliation: Research Center for Translational Medicine and Key Laboratory of Arrhythmias, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China.
authors.forename: Tianbao
authors.initials: T
authors.lastname: Lu
authors.affiliation: Department of Oncology, Shanghai East Hospital, Dalian Medical University, Shanghai 200120, China.|Research Center for Translational Medicine and Key Laboratory of Arrhythmias, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China.
authors.forename: Yujiao
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Department of Oncology, Shanghai East Hospital, Dalian Medical University, Shanghai 200120, China.|Department of Oncology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China.|Research Center for Translational Medicine and Key Laboratory of Arrhythmias, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China.|Departments of Microbiology/Immunology/Parasitology, Louisiana State University Health Sciences Center, Louisiana Cancer Research Center, New Orleans, LA 70112, USA.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Qin
authors.affiliation: Department of Oncology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China.|Research Center for Translational Medicine and Key Laboratory of Arrhythmias, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China.
authors.forename: Zengguang
authors.initials: Z
authors.lastname: Xu
mesh_terms: D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D039603:Eukaryotic Initiation Factor-4G / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D011379:Prognosis; D034741:RNA, Small Interfering / Q000235:genetics; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; C113920:EIF4G1 protein, human; D039603:Eukaryotic Initiation Factor-4G; D034741:RNA, Small Interfering
keywords: EIF4G1; NSCLC; USP10; lung cancer