title: Lipidomic profiling of dried seahorses by hydrophilic interaction chromatography coupled to mass spectrometry.
abstract: Dried seahorse is a precious raw food material for cooking soups. In this study, a lipidomics strategy using the techniques of solid-phase extraction (SPE) and hydrophilic interaction chromatography-tandem mass spectrometry (HILIC-QTOF/MS) was developed for extraction, visualization, and quantification of phospholipids in dried seahorses. The parameters of SPE were optimized, and 1 mL of sample and chloroform/methanol (1:2, v/v) were found to be the best loading volume and eluting solvent, respectively. Afterwards, each phospholipid class was successfully separated on a HILIC column and analyzed by mass spectrometry. A total of 50 phospholipid molecular species were identified and determined, including 15 phosphatidylcholines (PCs), 14 phosphatidylethanolamines (PEs), 12 phosphatidylinositols (PIs) and 9 phosphatidylserines (PSs). In comparison to previously methods, this strategy was robust and efficient in extraction, characterization, and determination of phospholipids. The dried seahorse was found to contain large amounts of polyunsaturated fatty acyl phospholipids which are beneficial to human health.
authors.affiliation: Institute of Seafood, Zhejiang Gongshang University, Hangzhou, China; Department of Biomedical Sciences, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Shen
authors.affiliation: Institute of Seafood, Zhejiang Gongshang University, Hangzhou, China. Electronic address: day@zjgsu.edu.cn.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Dai
authors.affiliation: Department of Food Science and Technology, University of Georgia, Athens, GA, United States.
authors.forename: Yao-Wen
authors.initials: YW
authors.lastname: Huang
authors.affiliation: Department of Biomedical Sciences, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China. Electronic address: bhhonyun@cityu.edu.hk.
authors.forename: Hon-Yeung
authors.initials: HY
authors.lastname: Cheung
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D010713:Phosphatidylcholines / Q000032:analysis / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis* / Q000737:chemistry; D017747:Seafood / Q000032:analysis*; D023701:Smegmamorpha; D052616:Solid Phase Extraction / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids
keywords: Hydrophilic interaction chromatography; Lipidomics; Quadruple/time-of-flight mass spectrometry; Seahorse; Solid-phase extraction