title: Effect of cobalt supplementation and fractionation on the biological response in the biomethanization of Olive Mill Solid Waste.
abstract: Due to the low trace metals concentration in the Olive Mill Solid Waste (OMSW), a proposed strategy to improve its biomethanization is the supplementation of key metals to enhance the microorganism activity. Among essential trace metals, cobalt has been reported to have a crucial role in anaerobic degradation. This study evaluates the effect of cobalt supplementation to OMSW, focusing on the connection between fractionation of cobalt in the system and the biological response. The highest biological responses was found in a range from 0.018 to 0.035mg/L of dissolved cobalt (0.24-0.65mg total cobalt/L), reaching improvements up to 23% and 30% in the methane production rate and the methane yield coefficient, respectively. It was found that the dissolved cobalt fraction is more accurately related with the biological response than the total cobalt. The total cobalt is distorted by the contribution of dissolved and non-dissolved inert fractions.
authors.affiliation: Master of Engineering Sciences with Specialization in Biotechnology, Universidad de La Frontera, Casilla 54-D, Temuco, Chile; Escuela de Procesos Industriales, Facultad de Ingeniería, Universidad Católica de Temuco, Casilla 15-D, Temuco, Chile.
authors.forename: F
authors.initials: F
authors.lastname: Pinto-Ibieta
authors.affiliation: Instituto de la Grasa (C.S.I.C.), Edificio 46, Campus Universitario Pablo de Olavide, Ctra. de Utrera, Km. 1, 41013 Seville, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Serrano
authors.affiliation: Departamento de Ingeniería Química, Universidad de La Frontera, Casilla 54-D, Temuco, Chile.
authors.forename: D
authors.initials: D
authors.lastname: Jeison
authors.affiliation: Instituto de la Grasa (C.S.I.C.), Edificio 46, Campus Universitario Pablo de Olavide, Ctra. de Utrera, Km. 1, 41013 Seville, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Borja
authors.affiliation: Instituto de la Grasa (C.S.I.C.), Edificio 46, Campus Universitario Pablo de Olavide, Ctra. de Utrera, Km. 1, 41013 Seville, Spain. Electronic address: fgfermoso@ig.csic.es.
authors.forename: F G
authors.initials: FG
authors.lastname: Fermoso
mesh_terms: D000693:Anaerobiosis; D019149:Bioreactors; D003035:Cobalt / Q000737:chemistry*; D008670:Metals; D008697:Methane / Q000032:analysis / Q000096:biosynthesis*; D031658:Olea / Q000737:chemistry* / Q000378:metabolism; D014131:Trace Elements / Q000378:metabolism; D018505:Waste Management / Q000379:methods
chemical_list: D008670:Metals; D014131:Trace Elements; D003035:Cobalt; D008697:Methane
keywords: Cobalt fractionation; Improved anaerobic digestion; Methane production rate; Olive Mill Solid Waste; Trace metal requirements