title: Oral administration of myostatin-specific recombinant Saccharomyces cerevisiae vaccine in rabbit.
abstract: Yeast is considered as a simple and cost-effective host for protein expression, and our previous studies have proved that Saccharomyces cerevisiae can deliver recombinant protein and DNA into mouse dendritic cells and can further induce immune responses as novel vaccines. In order to know whether similar immune responses can be induced in rabbit by oral administration of such recombinant S. cerevisiae vaccine, we orally fed the rabbits with heat-inactivated myostatin-recombinant S. cerevisiae for 5 weeks, and then myostatin-specific antibody in serum was detected successfully by western blotting and ELISA assay. The rabbits treated with myostatin-recombinant S. cerevisiae vaccine grew faster and their muscles were much heavier than that of the control group. As a common experimental animal and a meat livestock with great economic value, rabbit was proved to be the second animal species that have been successfully orally immunized by recombinant S. cerevisiae vaccine after mice.
authors.affiliation: College of Animal Science & Technology, Shaan'xi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shaan'xi 712100, China.
authors.forename: Zhongtian
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Animal Science & Technology, Shaan'xi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shaan'xi 712100, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Zhou
authors.affiliation: College of Animal Science & Technology, Shaan'xi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shaan'xi 712100, China.
authors.forename: Chonghua
authors.initials: C
authors.lastname: Ren
authors.affiliation: College of Animal Science & Technology, Shaan'xi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shaan'xi 712100, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Xu
authors.affiliation: College of Animal Science & Technology, Shaan'xi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shaan'xi 712100, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yan
authors.affiliation: College of Animal Science & Technology, Shaan'xi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shaan'xi 712100, China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Li
authors.affiliation: Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: College of Animal Science & Technology, Shaan'xi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shaan'xi 712100, China. Electronic address: zhangzhy@nwsuaf.edu.cn.
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000906:Antibodies / Q000097:blood; D055435:Myostatin / Q000276:immunology*; D011817:Rabbits; D011897:Random Allocation; D011994:Recombinant Proteins / Q000276:immunology; D012441:Saccharomyces cerevisiae; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D000906:Antibodies; D055435:Myostatin; D011994:Recombinant Proteins; D019444:Vaccines, DNA
keywords: Myostatin; Rabbit; Saccharomyces cerevisiae; Vaccine