title: HIV-1 Pol Gene Polymorphism and Antiretroviral Resistance Mutations in Treatment-Naive Adult Patients in French Guiana Between 2006 and 2012.
abstract: Little information is available on the molecular epidemiologic profile of HIV-1 in French Guiana, the French department with the highest HIV/AIDS incidence. To follow the evolution of HIV-1 diversity, we carried out a molecular analysis of HIV-1 isolates from 305 treatment-naive patients between 2006 and 2012. Protease and reverse-transcriptase sequences were obtained for subtype characterization, polymorphism analysis, and identification of drug resistance mutations. Of 305 HIV-1 strains, 95.1% were subtype B viruses. The overall prevalence of transmitted drug-resistance mutations (TDRMs) was 4.6% (14/305), ranging from 1.9% to 7.1% depending on the year. This study shows a low level of HIV-1 genetic diversity and a moderate prevalence of TDRMs with no evidence of an increasing trend over the study period. Nevertheless, the strong genetic polymorphism observed on both genes may be of concern for long-term treatment of people living with HIV-1 and thus deserves continuous monitoring.
authors.affiliation: 1 Laboratoire des Interactions Virus-Hôtes, Institut Pasteur de la Guyane , Cayenne Cedex, Guyane Française.
authors.forename: Edith
authors.initials: E
authors.lastname: Darcissac
authors.affiliation: 2 Hôpital de Jour Adultes, Centre Hospitalier de Cayenne "Andrée Rosemon ," Cayenne Cedex, Guyane Française.|3 Centre d'Investigation Clinique-Epidemiologie Clinique (CIC-EC) Antilles Guyane, INSERM CIE 802, Centre Hospitalier de Cayenne "Andrée Rosemon ," Cayenne Cedex, Guyane Française.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Nacher
authors.affiliation: 2 Hôpital de Jour Adultes, Centre Hospitalier de Cayenne "Andrée Rosemon ," Cayenne Cedex, Guyane Française.
authors.forename: Leila
authors.initials: L
authors.lastname: Adriouch
authors.affiliation: 4 Laboratoire de Biologie Médicale, Institut Pasteur de la Guyane , Cayenne Cedex, Guyane Française.
authors.forename: Alain
authors.initials: A
authors.lastname: Berlioz-Arthaud
authors.affiliation: 5 Service de Biologie Médicale, Centre Hospitalier de l'Ouest Guyanais "Franck Joly ," Saint Laurent du Maroni, Guyane Française.
authors.forename: Rachida
authors.initials: R
authors.lastname: Boukhari
authors.affiliation: 6 Service de Dermatologie, Centre Hospitalier de Cayenne "Andrée Rosemon ," Cayenne Cedex, Guyane Française.
authors.forename: Pierre
authors.initials: P
authors.lastname: Couppié
authors.affiliation: 7 Unité des Maladies Infectieuses et Tropicales, Centre Hospitalier de Cayenne "Andrée Rosemon ," Cayenne Cedex, Guyane Française.
authors.forename: Felix
authors.initials: F
authors.lastname: Djossou
authors.affiliation: 1 Laboratoire des Interactions Virus-Hôtes, Institut Pasteur de la Guyane , Cayenne Cedex, Guyane Française.
authors.forename: Damien
authors.initials: D
authors.lastname: Donato
authors.affiliation: 2 Hôpital de Jour Adultes, Centre Hospitalier de Cayenne "Andrée Rosemon ," Cayenne Cedex, Guyane Française.
authors.forename: Myriam
authors.initials: M
authors.lastname: El Guedj
authors.affiliation: 1 Laboratoire des Interactions Virus-Hôtes, Institut Pasteur de la Guyane , Cayenne Cedex, Guyane Française.
authors.forename: Anne
authors.initials: A
authors.lastname: Lavergne
authors.affiliation: 6 Service de Dermatologie, Centre Hospitalier de Cayenne "Andrée Rosemon ," Cayenne Cedex, Guyane Française.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Papot
authors.affiliation: 1 Laboratoire des Interactions Virus-Hôtes, Institut Pasteur de la Guyane , Cayenne Cedex, Guyane Française.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Pouliquen
authors.affiliation: 1 Laboratoire des Interactions Virus-Hôtes, Institut Pasteur de la Guyane , Cayenne Cedex, Guyane Française.
authors.forename: Edouard
authors.initials: E
authors.lastname: Tanguy
authors.affiliation: 8 Hôpital de Jour Adultes, Centre Hospitalier de l'Ouest Guyanais "Franck Joly ," Saint Laurent du Maroni, Guyane Française.
authors.forename: Vincent
authors.initials: V
authors.lastname: Vantilcke
authors.affiliation: 1 Laboratoire des Interactions Virus-Hôtes, Institut Pasteur de la Guyane , Cayenne Cedex, Guyane Française.
authors.forename: Vincent
authors.initials: V
authors.lastname: Lacoste
mesh_terms: D000328:Adult; D000368:Aged; D024882:Drug Resistance, Viral / Q000235:genetics; D005260:Female; D005616:French Guiana / Q000453:epidemiology; D005838:Genotype; D015658:HIV Infections / Q000188:drug therapy / Q000453:epidemiology* / Q000821:virology; D016333:HIV Protease / Q000235:genetics*; D017320:HIV Protease Inhibitors / Q000627:therapeutic use; D054303:HIV Reverse Transcriptase / Q000235:genetics*; D015497:HIV-1 / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D006801:Humans; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D009154:Mutation; D011110:Polymorphism, Genetic; D012189:Retrospective Studies; D018894:Reverse Transcriptase Inhibitors / Q000627:therapeutic use; D019562:Viral Load / Q000187:drug effects
chemical_list: D017320:HIV Protease Inhibitors; D018894:Reverse Transcriptase Inhibitors; D054303:HIV Reverse Transcriptase; D016333:HIV Protease