title: Evaluation of antibiofilm and mechanical properties of new nanocomposites based on acrylic resins and silver vanadate nanoparticles.
abstract: OBJECTIVE
  The purpose of this study was evaluate, for the first time, the impact of incorporation of nanostructured silver vanadate (β-AgVO3) in antibiofilm and mechanical properties of dental acrylic resins (poly(methyl methacrylate), PMMA).
  
  
  METHODS
  The β-AgVO3 was synthesized and characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy, and microanalysis (SEM/EDS). Resins specimens were prepared with 0-10% wt.% β-AgVO3 and characterized by SEM, XRD and optical microscopy. The antibiofim activity of the samples against Candida albicans and Streptococcus mutans was investigated by XTT reduction test, colony-forming units (CFUs), and confocal laser scanning microscopy (CLSM). The flexural strength, hardness, and surface roughness of the samples containing β-AgVO3 were compared with the pure PMMA matrix.
  
  
  RESULTS
  The incorporation of 10% β-AgVO3 significantly reduced the metabolic activity of C. albicans and S. mutans (p<0.05). There was a reduction in microbial load (CFU/mL) of microorganisms for the different concentrations used (p<0.05), which was confirmed by confocal microscopy. The addition of β-AgVO3 did not change the mechanical properties of hardness and surface roughness of the resins (p>0.05). However, flexural strength decreased with the addition of amounts greater than 1% (p<0.05).
  
  
  CONCLUSIONS
  β-AgVO3 additions in dental acrylic resin may have an impact on inhibition of biofilm of main microorganisms associated with dental prostheses. However, the viability of clinical use should be evaluated in function of changed promoted in some mechanical properties.
authors.affiliation: Department of Dental Materials and Prosthodontics, School of Dentistry of Ribeirão Preto, USP-University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Denise T
authors.initials: DT
authors.lastname: de Castro
authors.affiliation: Department of Dental Materials and Prosthodontics, School of Dentistry of Ribeirão Preto, USP-University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Mariana L C
authors.initials: ML
authors.lastname: Valente
authors.affiliation: Department of Dental Materials and Prosthodontics, School of Dentistry of Ribeirão Preto, USP-University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Cláudia H L
authors.initials: CH
authors.lastname: da Silva
authors.affiliation: Department of Dental Materials and Prosthodontics, School of Dentistry of Ribeirão Preto, USP-University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Evandro
authors.initials: E
authors.lastname: Watanabe
authors.affiliation: Postgraduate Program in Materials Science and Engineering-PPG-CEM, Federal University of São Carlos-UFSCAR, São Carlos, SP, Brazil.
authors.forename: Renato L
authors.initials: RL
authors.lastname: Siqueira
authors.affiliation: Department of Natural Sciences, GPQM -Research Group of Materials Chemistry, Federal University of São João del Rei-UFSJ, São João del Rei, MG, Brazil.
authors.forename: Marco A
authors.initials: MA
authors.lastname: Schiavon
authors.affiliation: Laboratory of Solid State Chemistry, State University of Campinas (UNICAMP), Campinas, Brazil.
authors.forename: Oswaldo Luiz
authors.initials: OL
authors.lastname: Alves
authors.affiliation: Department of Dental Materials and Prosthodontics, School of Dentistry of Ribeirão Preto, USP-University of São Paulo, Ribeirão Preto, SP, Brazil. Electronic address: andreare@forp.usp.br.
authors.forename: Andréa C
authors.initials: AC
authors.lastname: Dos Reis
mesh_terms: D000180:Acrylic Resins / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects / Q000254:growth & development; D003188:Composite Resins / Q000737:chemistry; D003764:Dental Materials / Q000737:chemistry; D006244:Hardness; D008422:Materials Testing; D053761:Nanocomposites / Q000737:chemistry*; D019904:Polymethyl Methacrylate / Q000737:chemistry / Q000494:pharmacology; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*; D013295:Streptococcus mutans / Q000187:drug effects / Q000254:growth & development; D014638:Vanadates / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000180:Acrylic Resins; D003188:Composite Resins; D003764:Dental Materials; D012834:Silver; D014638:Vanadates; D019904:Polymethyl Methacrylate
keywords: Acrylic resin; Antibiofilm properties; Mechanical behavior; Nanomaterials; Silver vanadate