title: Fluorescent aptasensor for chloramphenicol detection using DIL-encapsulated liposome as nanotracer.
abstract: A novel fluorescence aptasensor was successfully developed to respond to chloramphenicol (CAP) in food based on magnetic aptamer-liposome vesicle probe. In order to fabricate it, aptamer labeled on functionalized magnetic beads (MB) was firstly employed as capture adsorbent (MB-Apt), then SSB (single-stranded DNA binding protein) and DIL (1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanineperchlorate) coimmobilized liposomes (SSB/DIL-Lip) was employed as vesicle signal tracer. The composite vesicle probe is formed between SSB/DIL-Lip and MB-Apt based on SSB's specific recognition towards aptamer on vesicle signal tracer. Upon the vesicle probe solution reacted with CAP, the aptamer on the magnetic beads preferentially bounded with CAP, and then released SSB/DIL-Lip vesicle signal tracer in the supernatant after magnetic separation. The released tracer can emit fluorescence which was correspondence with the concentration of the analyte. At the optimum conditions, the aptasensor exhibited a good linear response for CAP detection in the range of 0.003-10nM with a detection limit of 1pM. Importantly, the methodology was further validated for analyzing CAP in fish samples with consistent results obtained by ELISA kit, thus providing a promising approach for quantitative monitoring of CAP and significant anti-interference ability in food safety.
authors.affiliation: State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.
authors.forename: Yang-Bao
authors.initials: YB
authors.lastname: Miao
authors.affiliation: Key Laboratory of Asymmetric Synthesis and Chirotechnology of Sichuan Province, Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041, PR China; University of Chinese Academy of Sciences, Beijing 10049, PR China.
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Ren
authors.affiliation: State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China. Electronic address: ganning@nbu.edu.cn.
authors.forename: Ning
authors.initials: N
authors.lastname: Gan
authors.affiliation: State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China. Electronic address: caoyuting@nbu.edu.cn.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Cao
authors.affiliation: State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.
authors.forename: Tianhua
authors.initials: T
authors.lastname: Li
authors.affiliation: Faculty of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210000, PR China.
authors.forename: Yijin
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D002701:Chloramphenicol / Q000032:analysis*; D005399:Fishes; D005456:Fluorescent Dyes / Q000737:chemistry; D005504:Food Analysis / Q000379:methods*; D055767:Immobilized Proteins / Q000737:chemistry; D057230:Limit of Detection; D008081:Liposomes / Q000737:chemistry; D059346:Magnets / Q000737:chemistry; D010472:Perchlorates / Q000737:chemistry; D017747:Seafood / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D000900:Anti-Bacterial Agents; D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D055767:Immobilized Proteins; D008081:Liposomes; D010472:Perchlorates; D002701:Chloramphenicol
keywords: Chloramphenicol; DIL; Fluorescence aptasensor; Magnetic liposome vesicle signal tracer; Single-stranded DNA binding protein