title: Protective effect of zingerone on increased vascular contractility in diabetic rat aorta.
abstract: The aim of the present study was to investigate the effect and possible mechanism of action of zingerone, the main constituent of ginger, on vascular reactivity in isolated aorta from diabetic rats. The results show that incubation of aortae with zingerone alleviates the exaggerated vasoconstriction of diabetic aortae to phenylephrine, as well as the impaired relaxatory response to acetylcholine in a concentration-dependent manner. Furthermore, Zingerone directly relax phenylephrine-precontracted aortae. The vasorelaxatory response is significantly attenuated by the nitric oxide synthase inhibitor Nω-nitro-l-arginine methyl ester hydrochloride and the guanylate cyclase inhibitor methylene blue but no effect of either the potassium channels blocker tetraethylammonium chloride, or the cyclooxygenase inhibitor indomethacin was observed. Zingerone had no effect on advanced glycation end product formation as well. In conclusion, zingerone ameliorates enhanced vascular contraction in diabetic aortae which may be mediated by its vasodilator effect through NO- and guanylate cyclase stimulation.
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Salah A
authors.initials: SA
authors.lastname: Ghareib
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia; Department of Pharmacology, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt.
authors.forename: Hany M
authors.initials: HM
authors.lastname: El-Bassossy
authors.affiliation: Department of Clinical Pharmacy, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia; Department of Pharmacology, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt. Electronic address: berry_ahmed@yahoo.com.
authors.forename: Ahmed A
authors.initials: AA
authors.lastname: Elberry
authors.affiliation: Department of Pediatric Cardiology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Azhar
authors.affiliation: Department of Pharmacy and Pharmacology, University of Bath, Bath, UK.
authors.forename: Malcolm L
authors.initials: ML
authors.lastname: Watson
authors.affiliation: Department of Clinical Biochemistry, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Zainy M
authors.initials: ZM
authors.lastname: Banjar
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt.
authors.forename: Abdulrahman M
authors.initials: AM
authors.lastname: Alahdal
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000503:physiopathology*; D017127:Glycation End Products, Advanced / Q000378:metabolism; D006139:Guaiacol / Q000031:analogs & derivatives* / Q000494:pharmacology; D008297:Male; D010656:Phenylephrine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D014661:Vasoconstriction / Q000187:drug effects*; D014664:Vasodilation / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology*
chemical_list: D017127:Glycation End Products, Advanced; D014665:Vasodilator Agents; D010656:Phenylephrine; C013738:zingerone; D006139:Guaiacol
keywords: Advanced glycation end products; Diabetes; Nitric oxide; Vascular complications; Vasorelaxant; Zingerone