title: Role of nitro-oxidative stress in the pathogenesis of experimental rat periodontitis.
abstract: OBJECTIVE
  Periodontitis is a common chronic adult condition that implicates oxidative damage to gingival tissue, periodontal ligament and alveolar bone. This study aimed at assessing the association between the nitro-oxidative stress and the periodontal tissues destructions in experimental rat periodontitis.
  
  
  METHODS
  Periodontitis was induced in 15 male albino rats by repetitive lesions to the gingiva adjacent to the inferior incisors, performed daily, for 16 days. On D1, D3, D6, D8, and D16 the onset and evolution of periodontitis were monitored by clinical and histopathological examinations; blood was collected and serum nitro-oxidative stress was evaluated through total nitrites and nitrates, total oxidative status, total antioxidant capacity, and oxidative stress index.
  
  
  RESULTS
  The results demonstrated that there was a graded and continuous increase in serum levels of total nitrites and nitrates, total oxidative status and oxidative stress index, which was consistent with the severity of periodontal destructions during periodontitis progression. However, total antioxidant capacity was not significantly influenced by the disease progression.
  
  
  CONCLUSIONS
  In experimental rat periodontitis, the systemic nitro-oxidative stress was associated with the severity of periodontal destructions assessed clinically and histopathologically. Therefore, systemic nitro-oxidative stress parameters might be used as diagnostic tools in periodontitis.
authors.affiliation: Department of Histology, Faculty of Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors.forename: Adina Bianca
authors.initials: AB
authors.lastname: Boşca
authors.affiliation: Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania.
authors.forename: Viorel
authors.initials: V
authors.lastname: Miclăuş
authors.affiliation: Department of Oral Rehabilitation, Oral Health and Dental Office Management, Faculty of Medical Dentistry, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors.forename: Aranka
authors.initials: A
authors.lastname: Ilea
authors.affiliation: Department of Oral Rehabilitation, Oral Health and Dental Office Management, Faculty of Medical Dentistry, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors.forename: Radu Septimiu
authors.initials: RS
authors.lastname: Câmpian
authors.affiliation: Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania.
authors.forename: Vasile
authors.initials: V
authors.lastname: Rus
authors.affiliation: Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania.
authors.forename: Flavia
authors.initials: F
authors.lastname: Ruxanda
authors.affiliation: Department of Oral Implantology, Faculty of Medical Dentistry, University of Oradea, Romania.
authors.forename: Cristian
authors.initials: C
authors.lastname: Raţiu
authors.affiliation: Department of Physiopathology, Faculty of Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors.forename: Ana
authors.initials: A
authors.lastname: Uifălean
authors.affiliation: Department of Physiopathology, Faculty of Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors.forename: Alina Elena
authors.initials: AE
authors.lastname: Pârvu
keywords: experimental periodontitis; nitro-oxidative stress; pathogenesis; rat