title: Combined effects of soy isoflavones and milk basic protein on bone mineral density in hind-limb unloaded mice.
abstract: We examined whether the combination of isoflavone and milk basic protein both are reported to be effective for bone metabolism, prevents bone loss induced by skeletal hind-limb unloading in mice. Female ddY strain mice, aged 8 weeks, were divided into six groups (n = 6-8 each): (1) normally housed group, (2) loading group, (3) hind-limb unloading group fed a control diet, (4) hind-limb unloading group fed a 0.2% isoflavone conjugates diet, (5) hind-limb unloading group fed a 1.0% milk basic protein diet, and (6) hind-limb unloading group fed a 0.2% isoflavone conjugates and 1.0% milk basic protein diet. After 3 weeks, femoral bone mineral density was markedly reduced in unloading mice. The combination of isoflavone and milk basic protein showed cooperative effects in preventing bone loss and milk basic protein inhibited the increased expression of osteogenic genes in bone marrow cells in unloading mice. These results suggest that the combination of soy isoflavone and milk basic protein may be useful for bone health in subjects with disabling conditions as well as astronauts.
authors.affiliation: Department of Food Function and Labeling, National Institute of Health and Nutrition, National Institutes of Biomedical Innovation, Health and Nutrition, Tokyo 162-8636, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Matsumoto
authors.affiliation: Department of Food Function and Labeling, National Institute of Health and Nutrition, National Institutes of Biomedical Innovation, Health and Nutrition, Tokyo 162-8636, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Tousen
authors.affiliation: Department of Food Function and Labeling, National Institute of Health and Nutrition, National Institutes of Biomedical Innovation, Health and Nutrition, Tokyo 162-8636, Japan.
authors.forename: Yoriko
authors.initials: Y
authors.lastname: Nishide
authors.affiliation: Department of Nutritional Science, Tokyo University of Agriculture, Tokyo 156-8502, Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Tadaishi
authors.affiliation: Milk Science Research Institute, Megmilk Snow Brand Co., Ltd., Saitama 350-1165, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Kato
authors.affiliation: Department of Food Function and Labeling, National Institute of Health and Nutrition, National Institutes of Biomedical Innovation, Health and Nutrition, Tokyo 162-8636, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Ishimi
keywords: bone mineral density; hindlimb-unloading; milk basic protein (MBP); soy isoflavones