title: Circulating tight junction proteins mirror blood-brain barrier integrity in leukaemia central nervous system metastasis.
abstract: The aim of this study was to evaluate the clinical significance of circulating tight junction (TJ) proteins as biomarkers reflecting of leukaemia central nervous system (CNS) metastasis. TJs [claudin5 (CLDN5), occludin (OCLN) and ZO-1] concentrations were measured in serum and cerebrospinal fluid (CSF) samples obtained from 45 leukaemia patients. Serum ZO-1 was significantly higher (p < 0.05), but CSF ZO-1 levels were not significantly higher in the CNS leukaemia (CNSL) compared to the non-CNSL. The CNSL patients also had a lower CLDN5/ZO1 ratio in both serum and CSF than in non-CNSL patients (p < 0.05). The TJ index was negatively associated with WBCCSF, ALBCSFand BBB values in leukaemia patients. Among all of the parameters studied, CLDN5CSFhad the highest specificity in discriminating between CNSL and non-CNSL patients. Therefore, analysing serum and CSF levels of CLDN5, OCLN and the CLDN5/ZO1 ratio is valuable in evaluating the potential of leukaemia CNS metastasis. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Cell Biology and Genetics, Shantou University Medical College, Shantou, China.|Shantou University Medical College Mental Health Center, Shantou, China.
authors.forename: Jing-Cheng
authors.initials: JC
authors.lastname: Zhu
authors.affiliation: Department of Cell Biology and Genetics, Shantou University Medical College, Shantou, China.
authors.forename: Meng-Ya
authors.initials: MY
authors.lastname: Si
authors.affiliation: Department of Cell Biology and Genetics, Shantou University Medical College, Shantou, China.
authors.forename: Ya-Zhen
authors.initials: YZ
authors.lastname: Li
authors.affiliation: Department of Cell Biology and Genetics, Shantou University Medical College, Shantou, China.
authors.forename: Huan-Zhu
authors.initials: HZ
authors.lastname: Chen
authors.affiliation: Department of Cell Biology and Genetics, Shantou University Medical College, Shantou, China.
authors.forename: Zhi-Cheng
authors.initials: ZC
authors.lastname: Fan
authors.affiliation: Department of Cell Biology and Genetics, Shantou University Medical College, Shantou, China.
authors.forename: Qing-Dong
authors.initials: QD
authors.lastname: Xie
authors.affiliation: Department of Cell Biology and Genetics, Shantou University Medical College, Shantou, China.
authors.forename: Xiao-Yang
authors.initials: XY
authors.lastname: Jiao
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers; D001812:Blood-Brain Barrier / Q000378:metabolism* / Q000473:pathology; D016543:Central Nervous System Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000401:mortality / Q000556:secondary*; D002648:Child; D062507:Claudin-5 / Q000097:blood / Q000134:cerebrospinal fluid; D005260:Female; D006801:Humans; D007938:Leukemia / Q000473:pathology*; D008297:Male; D062793:Occludin / Q000097:blood / Q000134:cerebrospinal fluid; D063868:Patient Outcome Assessment; D011379:Prognosis; D012372:ROC Curve; D062725:Tight Junction Proteins / Q000097:blood* / Q000134:cerebrospinal fluid; D055815:Young Adult; D062826:Zonula Occludens-1 Protein / Q000097:blood / Q000134:cerebrospinal fluid
chemical_list: D015415:Biomarkers; C107466:CLDN5 protein, human; D062507:Claudin-5; D062793:Occludin; C569042:TJP1 protein, human; D062725:Tight Junction Proteins; D062826:Zonula Occludens-1 Protein
keywords: ZO-1; claudin-5; leukaemia; occludin; tight junction protein