title: Neuropeptide Cycloprolylglycine Exhibits Neuroprotective Activity after Systemic Administration to Rats with Modeled Incomplete Global Ischemia and in In Vitro Modeled Glutamate Neurotoxicity.
abstract: We studied cerebroprotective properties of neuropeptide cycloprolylglycine (1 mg/kg) administered intraperitoneally to rats with modeled incomplete global ischemia rats and neuroprotective properties for HT-22 cells under conditions of glutamate toxicity. It was shown that the neuropeptide administered during the postischemic period restored the neurological status of rats by preventing sensorimotor impairments in the limb-placing test and suppression of locomotor activity in the open field test. In in vitro experiments, cycloprolylglycine in concentrations of 10(-5)-10(-8) M exhibited pronounced dose-dependent neuroprotective activity. The results attest to high cerebro- and neuroprotective potential of endogenous peptide cycloprolylglycine.
authors.affiliation: Laboratory of Peptide Bioregulators, Moscow, Russia. povarnina@gmail.com.
authors.forename: P Yu
authors.initials: PY
authors.lastname: Povarnina
authors.affiliation: Laboratory of Peptide Bioregulators, Moscow, Russia.
authors.forename: K N
authors.initials: KN
authors.lastname: Kolyasnikova
authors.affiliation: Laboratory of Pharmacology of Neuroprotection, V. V. Zakusov Research Institute of Pharmacology, Moscow, Russia.
authors.forename: S V
authors.initials: SV
authors.lastname: Nikolaev
authors.affiliation: Laboratory of Pharmacology of Neuroprotection, V. V. Zakusov Research Institute of Pharmacology, Moscow, Russia.
authors.forename: T A
authors.initials: TA
authors.lastname: Antipova
authors.affiliation: Laboratory of Peptide Bioregulators, Moscow, Russia.
authors.forename: T A
authors.initials: TA
authors.lastname: Gudasheva
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000188:drug therapy* / Q000503:physiopathology; D002460:Cell Line; D018698:Glutamic Acid / Q000633:toxicity*; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D020258:Neurotoxicity Syndromes / Q000188:drug therapy* / Q000503:physiopathology; D010456:Peptides, Cyclic / Q000494:pharmacology*; D051381:Rats
chemical_list: D018696:Neuroprotective Agents; D010456:Peptides, Cyclic; D018698:Glutamic Acid; C062238:cyclo(prolylglycyl)
keywords: cerebroprotective activity; glutamate neurotoxicity; incomplete global cerebral ischemia; neuropeptide cycloprolylglycine; neuroprotective activity