title: BHV-1 induced oxidative stress contributes to mitochondrial dysfunction in MDBK cells.
abstract: The levels of cellular reactive oxygen species (ROS) and ATP as well as the mitochondrial membrane potential (MMP) in response to bovine herpesvirus 1 (BHV-1) infection of MDBK cells were measured, respectively. BHV-1 infection increased ROS production which depended on viral entry, and de novo protein expression and/or DNA replication. Vice versa, excessive ROS was required for efficient viral replication. Levels of both ATP and MMP were significantly decreased after BHV-1 infection. Interestingly, the loss of MMP was ameliorated by ROS depression. Collectively, ROS dependent mitochondrial damage and ultimately disruption of energy metabolism (ATP depletion) are a potential pathogenic mechanism for BHV-1 infection.
authors.affiliation: College of Veterinary Medicine and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, 48 Wenhui East Road, Yangzhou, 225009, China.
authors.forename: Liqian
authors.initials: L
authors.lastname: Zhu
authors.affiliation: College of Veterinary Medicine and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, 48 Wenhui East Road, Yangzhou, 225009, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Yuan
authors.affiliation: College of Veterinary Medicine and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, 48 Wenhui East Road, Yangzhou, 225009, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, 48 Wenhui East Road, Yangzhou, 225009, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: College of Veterinary Medicine and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, 48 Wenhui East Road, Yangzhou, 225009, China.|The Test Center of Yangzhou University, 48 Wenhui East Road, Yangzhou, 225009, China.
authors.forename: Xiuyan
authors.initials: X
authors.lastname: Ding
authors.affiliation: College of Veterinary Medicine and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, 48 Wenhui East Road, Yangzhou, 225009, China. yzgqzhu@yzu.edu.cn.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism* / Q000821:virology; D004261:DNA Replication; D015870:Gene Expression; D006566:Herpesviridae Infections / Q000378:metabolism / Q000662:veterinary* / Q000821:virology; D007242:Herpesvirus 1, Bovine / Q000502:physiology*; D007241:Infectious Bovine Rhinotracheitis / Q000378:metabolism* / Q000821:virology; D008928:Mitochondria / Q000378:metabolism / Q000821:virology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D017382:Reactive Oxygen Species