title: Genotyping of microsatellite markers to study genetic structure of the wild striped snakehead Channa striata in Malaysia.
abstract: Genetic variability and differences in wild striped snakehead Channa striata from Malaysia were analysed by genotyping nine novel nuclear microsatellite loci. Analysis revealed moderate-to-high genetic diversity in most of the populations, indicative of large effective population sizes. The highly diversified populations are admixed populations and, therefore, can be recommended as potential candidates for selective breeding and conservation since they each contain most of the alleles found in their particular region. Three homogenous groups of the wild populations were identified, apparently separated by effective barriers, in accordance with contemporary drainage patterns. The highest population pairwise FST found between members of the same group reflects the ancient population connectivity; yet prolonged geographical isolation resulted in adaptation of alleles to local contemporary environmental change. A significant relationship between genetic distance and geographical isolation was observed (r = 0·644, P < 0·01). Anthropogenic perturbations indicated apparent genetic proximity between distant populations.
authors.affiliation: School of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu, 21030, Kuala Terengganu, Terengganu, Malaysia.|Institut Akuakultur Tropika (AKUATROP), Universiti Malaysia Terengganu, 21030, Kuala Terengganu, Terengganu, Malaysia.|School of Biological Sciences, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia.
authors.forename: M P
authors.initials: MP
authors.lastname: Tan
authors.affiliation: School of Biological Sciences, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia.
authors.forename: A F J
authors.initials: AF
authors.lastname: Jamsari
authors.affiliation: School of Biological Sciences, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia.
authors.forename: M N
authors.initials: MN
authors.lastname: Siti Azizah
mesh_terms: D000483:Alleles; D000818:Animals; D051456:Gene Flow; D014644:Genetic Variation; D005838:Genotype; D060005:Genotyping Techniques; D005843:Geography; D008296:Malaysia; D018895:Microsatellite Repeats; D010473:Perciformes / Q000235:genetics*; D058974:Phylogeography
keywords: anthropogenic factor; conservation; freshwater fish; genetic diversity; isolation by distance; nuclear DNA