title: Arabidopsis DREB1B in transgenic Salvia miltiorrhiza increased tolerance to drought stress without stunting growth.
abstract: Multiple stress response genes are controlled by transcription factors in a coordinated manner; therefore, these factors can be used for molecular plant breeding. CBF1/DREB1B, a known stress-inducible gene, was isolated from Arabidopsis thaliana and introduced into Salvia miltiorrhiza under the control of the CaMV35S or RD29A promoter. Under drought stress, relative water content, chlorophyll content, and the net photosynthetic rate were observed to be higher in the transgenic lines than in the wild type (WT). Moreover, O2(-) and H2O2 accumulation was observed to be lower in the transgenic lines. Additional analyses revealed that the AtDREB1B transgenic plants generally displayed lesser malondialdehyde (MDA) but higher superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activities than the WT under drought stress. Quantitative real-time polymerase chain reaction of a subset of genes involved in photosynthesis, stress response, carbohydrate metabolism, and cell protection further verified that AtDREB1B could enhance tolerance to drought by activating different downstream DREB/CBF genes in the transgenic plants. Furthermore, no growth inhibition was detected in transgenic S. miltiorrhiza plants that expressed AtDREB1B driven by either the constitutive CaMV35S promoter or the stress-inducible RD29A promoter. Together, these results suggest that AtDREB1B is a good candidate gene for increasing drought tolerance in transgenic S. miltiorrhiza.
authors.affiliation: College of Life Sciences, Nankai University, Tianjin, 300071, PR China; School of Life Sciences and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wei
authors.affiliation: School of Life Sciences and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, PR China.
authors.forename: Kejun
authors.initials: K
authors.lastname: Deng
authors.affiliation: College of Life Sciences, Nankai University, Tianjin, 300071, PR China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Gao
authors.affiliation: School of Life Sciences and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Nankai University, Tianjin, 300071, PR China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Yang
authors.affiliation: College of Life Sciences, Nankai University, Tianjin, 300071, PR China.
authors.forename: Lipeng
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Life Sciences and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, PR China.
authors.forename: Xuelian
authors.initials: X
authors.lastname: Zheng
authors.affiliation: College of Life Sciences, Nankai University, Tianjin, 300071, PR China.
authors.forename: Chunguo
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Nankai University, Tianjin, 300071, PR China.
authors.forename: Wenqin
authors.initials: W
authors.lastname: Song
authors.affiliation: College of Life Sciences, Nankai University, Tianjin, 300071, PR China. Electronic address: chencb@nankai.edu.cn.
authors.forename: Chengbin
authors.initials: C
authors.lastname: Chen
authors.affiliation: School of Life Sciences and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, PR China. Electronic address: zhangyong916@uestc.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002734:Chlorophyll / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D008315:Malondialdehyde / Q000378:metabolism; D010788:Photosynthesis; D030821:Plants, Genetically Modified; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D027561:Salvia miltiorrhiza / Q000201:enzymology / Q000235:genetics / Q000254:growth & development* / Q000502:physiology*; D013312:Stress, Physiological / Q000235:genetics; D014157:Transcription Factors / Q000378:metabolism*; D019076:Transgenes; D014867:Water / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; C114578:DREB1A protein, Arabidopsis; D017382:Reactive Oxygen Species; C036483:T-DNA; D014157:Transcription Factors; D014867:Water; D002734:Chlorophyll; D008315:Malondialdehyde
keywords: Antioxidant defense system; AtDREB1B; Drought; Photosynthesis; Salvia miltiorrhiza