title: Effects of 4-META/MMA-TBB Resin at Different Curing Stages on Osteoblasts and Gingival Epithelial Cells.
abstract: OBJECTIVE
  To assess the effects of different curing stages of 4-META/MMA-TBB resin on osteoblasts and gingival keratinocytes.
  
  
  METHODS
  The MC3T3-E1 murine pre-osteoblastic cell line and GE-1 murine gingival epithelial cell line were cultured with mixtures of Super-Bond C&B at different curing stages, and the cell viability was assessed. The alkaline phosphatase (ALP) activity of the MC3T3-E1 cells was also assessed.
  
  
  RESULTS
  The majority of the MC3T3-E1 cells died and showed no ALP activity when cultured with 4-META/MMA-TBB resin during the initial curing phase (1 min of curing). A later curing phase of the 4-META/MMA-TBB resin (7 min of curing) showed cytotoxicity at day 1, but the toxic effect was temporary and the proliferative capacity and ALP activity in the cells were similar to control cells at day 7. Completely cured 4-META/MMA-TBB resin (after 1 or 12 h of curing) did not affect the cell viability or ALP activity of the MC3T3-E1 cells. In contrast, 4-META/MMA-TBB resin showed no effect on the GE-1 cells at any stage of curing.
  
  
  CONCLUSIONS
  Although 4-META/MMA-TBB resin during the initial curing phase shows toxic effects on MC3T3-E1 cells, that cytotoxicity is minimal at later curing phases. In contrast, neither the uncured nor cured resins affected the GE-1 cells.
authors.forename: Takahiko
authors.initials: T
authors.lastname: Morotomi
authors.forename: Shizu
authors.initials: S
authors.lastname: Hirata-Tsuchiya
authors.forename: Ayako
authors.initials: A
authors.lastname: Washio
authors.forename: Chiaki
authors.initials: C
authors.lastname: Kitamura
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001896:Boron Compounds / Q000737:chemistry* / Q000494:pharmacology; D018929:Cell Culture Techniques; D016923:Cell Death; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005881:Gingiva / Q000166:cytology / Q000187:drug effects*; D015603:Keratinocytes / Q000187:drug effects*; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry* / Q000494:pharmacology; D008768:Methylmethacrylates / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects*; D019279:Resin Cements / Q000737:chemistry* / Q000494:pharmacology; D013997:Time Factors
chemical_list: D001896:Boron Compounds; D008689:Methacrylates; D008768:Methylmethacrylates; D019279:Resin Cements; C049495:Super-bond; D000469:Alkaline Phosphatase