title: Development and validation of an LC-ESI-MS/MS method for the simultaneous quantification of naproxen and sumatriptan in human plasma: application to a pharmacokinetic study.
abstract: A sensitive and fast liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method was developed and validated for the simultaneous quantification of naproxen and sumatriptan in human plasma. A simple liquid-liquid extraction procedure, with a mixture of ethyl acetate, methyl tert-butyl ether, and dichloromethane (4:3:3, v/v), was used for the cleanup of plasma. Naratriptan and aceclofenac were employed as internal standards. The analyses were carried out using an ACE C18 column (50 × 4.6 mm i.d.; particle size 5 μm) and a mobile phase consisting of 2 mM aqueous ammonium acetate with 0.025 % formic acid and methanol (38:62, v/v). A triple-quadrupole mass spectrometer equipped with an electrospray source in the positive mode was set up in the selective reaction monitoring mode to detect the ion transitions m/z 231.67 → m/z 185.07, m/z 296.70 → m/z 157.30, m/z 354.80 → m/z 215.00, and m/z 336.80 → m/z 97.94 for naproxen, sumatriptan, aceclofenac, and naratriptan, respectively. The method was validated and proved to be linear, accurate, precise, and selective over the ranges of 2.5-130 μg mL(-1) for naproxen and 1-50 ng mL(-1) for sumatriptan. The validated method was successfully applied to a pharmacokinetic study with simultaneous administration of naproxen sodium and sumatriptan succinate tablet formulations in healthy volunteers.
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Minas Gerais (UFMG), Avenida Presidente Antônio Carlos 6627, Belo Horizonte, Minas Gerais, 31.270-901, Brazil.
authors.forename: Juliana Machado
authors.initials: JM
authors.lastname: Brêtas
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Minas Gerais (UFMG), Avenida Presidente Antônio Carlos 6627, Belo Horizonte, Minas Gerais, 31.270-901, Brazil. isacesar@gmail.com.
authors.forename: Isabela Costa
authors.initials: IC
authors.lastname: César
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Minas Gerais (UFMG), Avenida Presidente Antônio Carlos 6627, Belo Horizonte, Minas Gerais, 31.270-901, Brazil.
authors.forename: Camila Machado
authors.initials: CM
authors.lastname: Brêtas
authors.affiliation: Instituto de Ciências Farmacêuticas, Alameda Coronel Eugênio Jardim 53, Aparecida de Goiânia, Goiás, 74.175-100, Brazil.
authors.forename: Leonardo de Souza
authors.initials: Lde S
authors.lastname: Teixeira
authors.affiliation: Instituto de Ciências Farmacêuticas, Alameda Coronel Eugênio Jardim 53, Aparecida de Goiânia, Goiás, 74.175-100, Brazil.
authors.forename: Karini Bruno
authors.initials: KB
authors.lastname: Bellorio
authors.affiliation: Instituto de Ciências Farmacêuticas, Alameda Coronel Eugênio Jardim 53, Aparecida de Goiânia, Goiás, 74.175-100, Brazil.
authors.forename: Iram Moreira
authors.initials: IM
authors.lastname: Mundim
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Minas Gerais (UFMG), Avenida Presidente Antônio Carlos 6627, Belo Horizonte, Minas Gerais, 31.270-901, Brazil.
authors.forename: Gerson Antônio
authors.initials: GA
authors.lastname: Pianetti
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D009288:Naproxen / Q000097:blood* / Q000493:pharmacokinetics; D010949:Plasma / Q000737:chemistry; D018170:Sumatriptan / Q000097:blood* / Q000493:pharmacokinetics; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009288:Naproxen; D018170:Sumatriptan
keywords: Human plasma; LC-ESI-MS/MS; Naproxen; Pharmacokinetics; Sumatriptan