title: Immunoreactivity, sensory and physicochemical properties of fermented soy protein isolate.
abstract: The effect of induced liquid state fermentation (Bacillus subtilis, Rhizopus oryzae, Saccharomyces cerevisiae, Lactobacillus helveticus) on the immunoreactivity, physicochemical and sensory properties of soy protein isolate (SPI) was studied. L. helveticus revealed the most abundant reduction in terms of immunoreactivity within soluble protein fractions, up to 100%, which could be measured by in vitro sandwich ELISA using mouse monoclonal anti-Glym5 antibodies (mAbs). Almost no binding was found in western blot analysis using mouse monoclonal mAbs and sera from soy sensitive individuals. Fermentation increased water- and oil-binding capacity as well as protein solubility at pH 4.0. Foaming activity was nearly doubled compared to non-fermented SPI. A decreased emulsifying capacity, foaming density, and quantity of soluble proteins at pH 7.0 were observed. Principal component analysis (PCA) confirmed decreased bitter and beany off-flavors of fermented samples compared to non-fermented SPI. Consequently, fermentation might be a promising method to produce tasty low-allergen food ingredients with good physicochemical properties.
authors.affiliation: Fraunhofer Institute for Process Engineering and Packaging (IVV), Giggenhauser Str. 35, Freising, Germany. Electronic address: pia.meinlschmidt@ivv.fraunhofer.de.
authors.forename: Pia
authors.initials: P
authors.lastname: Meinlschmidt
authors.affiliation: Fraunhofer Institute for Cell Therapy and Immunology (IZI), Perlickstr. 1, Leipzig, Germany.
authors.forename: Elke
authors.initials: E
authors.lastname: Ueberham
authors.affiliation: Fraunhofer Institute for Cell Therapy and Immunology (IZI), Perlickstr. 1, Leipzig, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Lehmann
authors.affiliation: Fraunhofer Institute for Process Engineering and Packaging (IVV), Giggenhauser Str. 35, Freising, Germany.
authors.forename: Ute
authors.initials: U
authors.lastname: Schweiggert-Weisz
authors.affiliation: Fraunhofer Institute for Process Engineering and Packaging (IVV), Giggenhauser Str. 35, Freising, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Eisner
mesh_terms: D000818:Animals; D015153:Blotting, Western; D055598:Chemical Phenomena; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005285:Fermentation; D006801:Humans; D051379:Mice; D025341:Principal Component Analysis / Q000379:methods*; D012995:Solubility; D030262:Soybean Proteins / Q000737:chemistry*
chemical_list: D030262:Soybean Proteins
keywords: Fermentation; Physicochemical properties; Principal component analysis (PCA); Sandwich ELISA and western blot; Soybean allergy