title: Antioxidant activities of Eryngium caucasicum inflorescence.
abstract: OBJECTIVE
  Medicinal plants are good sources for discovery of safe and new antioxidants. The aim of present study was to evaluate the efficiencies of three methods for evaluated for antioxidants from Eryngium caucasicum.
  
  
  METHODS
  Ultrasonically assisted extraction, Soxhlet extraction and percolation method were evaluated for the extraction of antioxidants from E. caucasicum inflorescence. Antioxidant activities of extracts were evaluated with four different in vitro tests.
  
  
  RESULTS
  Soxhlet extract had higher total phenolic and flavonoid contents than other extracts. Soxhlet extract showed the highest activity in DPPH radical scavenging (IC50 = 83.1 ± 2.1 µg ml-1). Soxhlet extract showed the best activity in iron chelatory (IC50 = 272 ± 6.3 µg ml-1) followed by ultrasonic extract. Percolation extract showed higher NO radical scavenging (IC50 = 390 ± 11.4 µg ml-1) than other extracts. Extracts showed good reducing power (p > 0.05).
  
  
  CONCLUSIONS
  The results obtained indicated that all three extraction methods especially Soxhlet method could effectively extract antioxidants from this plant.
authors.affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Fars, Iran. zadeh20@gmail.com.
authors.forename: S
authors.initials: S
authors.lastname: Motallebi Riekandeh
authors.forename: M
authors.initials: M
authors.lastname: Mazandarani
authors.forename: M A
authors.initials: MA
authors.lastname: Ebrahimzadeh
authors.forename: M
authors.initials: M
authors.lastname: Zargari
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000737:chemistry; D031049:Eryngium; D005419:Flavonoids / Q000032:analysis; D056626:Inflorescence; D007501:Iron / Q000737:chemistry; D009569:Nitric Oxide / Q000737:chemistry; D010084:Oxidation-Reduction; D010636:Phenols / Q000032:analysis; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D009569:Nitric Oxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D007501:Iron