title: Fast detection of coliform bacteria by means of gas chromatography-differential mobility spectrometry.
abstract: In this study, we demonstrate that the combination of an enzymatic method (based on Colilert-18 medium) and gas chromatography-differential mobility spectrometry (GC-DMS) can reduce the time required for detection of coliform bacteria (including Escherichia coli) from 18 to 2.5 h. The presented method includes the incubation (~2.5 h) of the sample containing coliform bacteria in Colilert-18 medium. The incubation time of 2.5 h is required for the activation of the β-galactosidase enzyme. Produced during the incubation biomarker o-nitrophenol (ONP) can be detected by means of GC-DMS within just 200 s. The detection limit for ONP was 45 ng (on-column). The method developed in this work provides significantly shorter analysis time compared with standard methods, and can be potentially adapted to the field conditions. Therefore, this method is a promising tool for an early detection of coliform bacteria (including E. coli). Graphical Abstract Fast detection of coliform bacteria by means of GC-DMS.
authors.affiliation: Faculty of Chemistry, Instrumental Analytical Chemistry, University of Duisburg-Essen, Universitätsstr. 5, 45141, Essen, Germany. lenasaptalena@gmail.com.
authors.forename: Lena Ganda
authors.initials: LG
authors.lastname: Saptalena
authors.affiliation: Faculty of Chemistry, Instrumental Analytical Chemistry, University of Duisburg-Essen, Universitätsstr. 5, 45141, Essen, Germany. andriy.kuklya@uni-due.de.
authors.forename: Andriy
authors.initials: A
authors.lastname: Kuklya
authors.affiliation: Faculty of Chemistry, Instrumental Analytical Chemistry, University of Duisburg-Essen, Universitätsstr. 5, 45141, Essen, Germany.|IWW Water Centre, Moritzstr. 26, 45476, Mülheim a.d. Ruhr, Germany.
authors.forename: Ursula
authors.initials: U
authors.lastname: Telgheder
mesh_terms: D002849:Chromatography, Gas / Q000379:methods*; D004755:Enterobacteriaceae / Q000302:isolation & purification*; D057230:Limit of Detection; D013057:Spectrum Analysis / Q000379:methods*
keywords: Bacteria detection; Coliform bacteria; Differential mobility mpectrometry (DMS); Enzymatic method; Escherichia coli (E. coli); MicroAnalyzer