title: Cytochrome P450 CYP6DA2 regulated by cap 'n'collar isoform C (CncC) is associated with gossypol tolerance in Aphis gossypii Glover.
abstract: Cotton plants accumulate phytotoxins, such as gossypol and related sesquiterpene aldehydes, to resist insect herbivores. The survival of insects exposed to toxic secondary metabolites depends on the detoxification metabolism mediated by limited groups of cytochrome P450. Gossypol has an antibiotic effect on Aphis gossypii, and as the concentrations of gossypol were increased in the present study, the mortality of cotton aphids increased from 4 to 28%. The fecundity of the cotton aphids exposed to gossypol was also significantly reduced compared with the control. The transcriptional levels of CYP6DA2 in cotton aphids were significantly induced when exposed to gossypol, and knockdown of the CYP6DA2 transcripts by RNA interference (RNAi) significantly increased the toxicity of gossypol to cotton aphids. To further understand the gossypol regulatory cascade, the 5'-flanking promoter sequences of CYP6DA2 were isolated with a genome walker, and the promoter was very active and was inducible by gossypol. Co-transfection of the cap 'n' collar isoform C (CncC) and CYP6DA2 promoters dramatically increased the expression of CYP6DA2, and suppression of the CncC transcripts by RNAi significantly decreased the expression levels of CYP6DA2, and significantly increased the toxicity of gossypol to cotton aphids. Thus, the transcriptional regulation of CYP6DA2 involved the transcriptional factor CncC.
authors.affiliation: College of Plant Science, Jilin University, Changchun, China.
authors.forename: T
authors.initials: T
authors.lastname: Peng
authors.affiliation: College of Plant Science, Jilin University, Changchun, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Department of Entomology, China Agricultural University, Beijing, China.
authors.forename: X
authors.initials: X
authors.lastname: Gao
authors.affiliation: College of Plant Science, Jilin University, Changchun, China.
authors.forename: J
authors.initials: J
authors.lastname: Xi
authors.affiliation: Department of Entomology, China Agricultural University, Beijing, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Plant Science, Jilin University, Changchun, China.
authors.forename: C
authors.initials: C
authors.lastname: Yang
authors.affiliation: College of Plant Science, Jilin University, Changchun, China.|Department of Entomology, Jilin Agricultural University, Changchun, China.
authors.forename: R
authors.initials: R
authors.lastname: Bi
authors.affiliation: College of Plant Science, Jilin University, Changchun, China.
authors.forename: S
authors.initials: S
authors.lastname: Yang
authors.affiliation: College of Plant Science, Jilin University, Changchun, China.
authors.forename: X
authors.initials: X
authors.lastname: Xin
authors.affiliation: College of Plant Science, Jilin University, Changchun, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Shang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005298:Fertility; D005786:Gene Expression Regulation; D003368:Gossypium / Q000737:chemistry; D006072:Gossypol / Q000378:metabolism; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D019476:Insect Proteins; D007527:Isoenzymes; D020033:Protein Isoforms; D012097:Repressor Proteins; D003577:Cytochrome P-450 Enzyme System; D006072:Gossypol
keywords: Aphis gossypii; cytochrome P450; gossypol; transcriptional regulation