title: Carbon dots for naked eye colorimetric ultrasensitive arsenic and glutathione detection.
abstract: A novel one-step method for the synthesis of bright, multicolor fluorescent sulphur doped carbon dots (CNDs) has been developed by using simple microwave assisted pyrolysis of citric acid and sodium thiosulphate. The synthesized CNDs showed dual mode naked eye colorimetric ultrasensitive sensing capability both for arsenic [As (III)] and glutathione (GSH) with high selectivity. Using fluorometric assay, the detection limit (DL) for As (III) was found to be as low as 32pM. The selectivity data show that the newly developed CNDs is very specific for As (III) even with interference by high concentrations of other metal ions. The CNDs were also able to detect GSH very selectively over other biothiols like cysteine (Cys) and homo-cysteine (H-cys) with a DL of 43nM, even in blood plasma. The fast kinetic data suggests that the present CNDs assay could be used onsite As (III) detection. The CNDs, further, showed its potential application in high resolution bioimaging of bacterial nucleoid segregation.
authors.affiliation: School of Basic Sciences, Indian Institute of Technology Mandi, Himachal Pradesh, India.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Gupta
authors.affiliation: School of Basic Sciences, Indian Institute of Technology Mandi, Himachal Pradesh, India.
authors.forename: Navneet Chandra
authors.initials: NC
authors.lastname: Verma
authors.affiliation: School of Basic Sciences, Indian Institute of Technology Mandi, Himachal Pradesh, India.
authors.forename: Syamantak
authors.initials: S
authors.lastname: Khan
authors.affiliation: School of Basic Sciences, Indian Institute of Technology Mandi, Himachal Pradesh, India. Electronic address: chayan@iitmandi.ac.in.
authors.forename: Chayan Kanti
authors.initials: CK
authors.lastname: Nandi
mesh_terms: D001151:Arsenic / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods; D002244:Carbon / Q000737:chemistry*; D003124:Colorimetry / Q000379:methods*; D005978:Glutathione / Q000032:analysis / Q000097:blood*; D006801:Humans; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D013050:Spectrometry, Fluorescence / Q000379:methods; D014867:Water / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D014874:Water Pollutants, Chemical; D014867:Water; D002244:Carbon; D005978:Glutathione; D001151:Arsenic
keywords: Arsenic and glutathione detection; Bacterial Nucleoid division; Carbon dots; Colorimetric dual mode sensor