title: Ultrastructural description of Myxobolus cuneus (Myxosporea) in the skeletal muscle and kidney of tropical farmed fish Piaractus mesopotamicus (Characiformes: Characidae).
abstract: This study characterizes by transmission electron microscopy (TEM) and morphometric features the myxozoan Myxobolus cuneus (Myxosporea) in Piaractus mesopotamicus and reports the skeletal muscle and kidney as site of infection. The register was based in 21 young fish from intensive fish farming in Southeast Brazil and the spores were analyzed in fresh-mounted slides of the infected organs stained with Toluidine blue and processed as usual for TEM. It differs from Myxobolus cunhai from the fish host and different polar capsule size, and from Myxobolus serrasalmi on the pyriform spore shape and an oval macrospore, differently to that reported in this study. Morphometric characteristics and TEM study confirmed the present material as M. cuneus.
authors.affiliation: Department of Veterinary Pathology, Camilo Castelo Branco University, Descalvado Campus. Av. Hilário da Silva Passos, Descalvado, CEP: 13690-000, São Paulo, Brazil. wilsongomezmanrique@yahoo.es.
authors.forename: Wilson Gómez
authors.initials: WG
authors.lastname: Manrique
authors.affiliation: Department of Parasitology, Institute for Biomedical Sciences, University of São Paulo, São Paulo, 05508-000, Brazil.
authors.forename: Mayra Araguaia Pereira
authors.initials: MA
authors.lastname: Figueiredo
authors.affiliation: Department of Veterinary Pathology, Camilo Castelo Branco University, Descalvado Campus. Av. Hilário da Silva Passos, Descalvado, CEP: 13690-000, São Paulo, Brazil.|Department of Preventive Veterinary Medicine, São Paulo State University, Via de acesso Prof. Paulo Donato Castellane s/n, Jaboticabal, 14884-900, São Paulo, Brazil.
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: de Andrade Belo
authors.affiliation: Aquatic Organisms Health Laboratory, Aquaculture Department, Federal University of Santa Catarina, Rod. Admar Gonzaga 1346, Florianópolis, 88040-900, Santa Catarina, Brazil.
authors.forename: Maurício Laterça
authors.initials: ML
authors.lastname: Martins
authors.affiliation: Department of Cell Biology, Institute of Biomedical Sciences, University of Porto, Largo Prof. Abel Salazar 2, 4099-003, Porto, Portugal.
authors.forename: Carlos
authors.initials: C
authors.lastname: Azevedo
mesh_terms: D000818:Animals; D001938:Brazil; D060126:Characidae / Q000469:parasitology*; D005393:Fish Diseases / Q000469:parasitology*; D007668:Kidney / Q000469:parasitology; D046529:Microscopy, Electron, Transmission; D018482:Muscle, Skeletal / Q000469:parasitology; D055766:Myxobolus / Q000302:isolation & purification / Q000648:ultrastructure*; D055764:Myxozoa / Q000469:parasitology*; D009778:Occipital Lobe / Q000469:parasitology; D010273:Parasitic Diseases, Animal / Q000469:parasitology*; D013170:Spores; D033761:Spores, Protozoan / Q000648:ultrastructure*
keywords: Freshwater fish; Myxozoa; Pacu; Transmission electron microscopy