title: A capillary electrophoresis system with dual capacitively coupled contactless conductivity detection and electrospray ionization tandem mass spectrometry.
abstract: A commercial system that is comprised of a CE coupled to an ESI triple quadrupole mass spectrometer was equipped with two capacitively coupled contactless conductivity detectors (C(4) Ds). The first C(4) D was positioned inside the original cartridge, and the second C(4) D was positioned as close as possible to the ESI probe entrance by using a 3D-printed support. The C(4) Ds electropherograms were matched to the ESI-MS electropherogram by correcting their timescales by the factor LT /LD , where LT and LD are the total capillary length and the length until the C(4) D, respectively. A general approach for method development supporting the simultaneous conductivity and MS detection is discussed, while application examples are introduced. These examples include the use of C(4) D as a simple device that dismiss the use of an EOF marker, a low-selectivity detector that continuously provide information about unexpected features of the sample, and even a detector that can be more sensitive than ESI-MS. The C(4) D used in this setup proved to have a smaller contribution to the peak broadening than ESI-MS, which allowed that a C(4) D, positioned at 12 cm from the inlet of an 80-cm-long capillary, could be used to foresee position and shape of the peaks being formed 6.8 times slower at the ESI-MS electropherogram.
authors.affiliation: Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, São Paulo - SP, CEP, Brazil.
authors.forename: Kelliton José Mendonça
authors.initials: KJ
authors.lastname: Francisco
authors.affiliation: Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, São Paulo - SP, CEP, Brazil.
authors.forename: Claudimir Lucio
authors.initials: CL
authors.lastname: do Lago
mesh_terms: D004553:Electric Conductivity; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods; D053719:Tandem Mass Spectrometry
keywords: Conductivity detection; Electrospray ionization; Mass spectrometry