title: Recent Advances in Clinical Glycoproteomics of Immunoglobulins (Igs).
abstract: Antibody glycosylation analysis has seen methodological progress resulting in new findings with regard to antibody glycan structure and function in recent years. For example, antigen-specific IgG glycosylation analysis is now applicable for clinical samples because of the increased sensitivity of measurements, and this has led to new insights in the relationship between IgG glycosylation and various diseases. Furthermore, many new methods have been developed for the purification and analysis of IgG Fc glycopeptides, notably multiple reaction monitoring for high-throughput quantitative glycosylation analysis. In addition, new protocols for IgG Fab glycosylation analysis were established revealing autoimmune disease-associated changes. Functional analysis has shown that glycosylation of IgA and IgE is involved in transport across the intestinal epithelium and receptor binding, respectively.
authors.affiliation: From the ‡Leiden University Medical Center, Center for Proteomics and Metabolomics, Leiden, The Netherlands;
authors.forename: Rosina
authors.initials: R
authors.lastname: Plomp
authors.affiliation: From the ‡Leiden University Medical Center, Center for Proteomics and Metabolomics, Leiden, The Netherlands; §Leiden University Medical Center, Department of Rheumatology, Leiden, The Netherlands;
authors.forename: Albert
authors.initials: A
authors.lastname: Bondt
authors.affiliation: From the ‡Leiden University Medical Center, Center for Proteomics and Metabolomics, Leiden, The Netherlands;
authors.forename: Noortje
authors.initials: N
authors.lastname: de Haan
authors.affiliation: ¶Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, UPS, France.
authors.forename: Yoann
authors.initials: Y
authors.lastname: Rombouts
authors.affiliation: From the ‡Leiden University Medical Center, Center for Proteomics and Metabolomics, Leiden, The Netherlands; m.wuhrer@lumc.nl.
authors.forename: Manfred
authors.initials: M
authors.lastname: Wuhrer
mesh_terms: D000941:Antigens / Q000378:metabolism; D001327:Autoimmune Diseases / Q000378:metabolism*; D001692:Biological Transport; D006031:Glycosylation; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry*; D007074:Immunoglobulin G / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D006023:Glycoproteins
chemical_list: D000941:Antigens; D007070:Immunoglobulin A; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; C042441:glycosylated IgA; C046179:glycosylated IgG; D006023:Glycoproteins