title: Crenalkalicoccus roseus gen. nov., sp. nov., a thermophilic bacterium isolated from alkaline hot springs.
abstract: Two closely related thermophilic bacterial strains, designated YIM 78023T and YIM 78058, were isolated from samples collected from two alkaline hot springs in Tengchong county, Yunnan province, south-west China. The novel isolates were Gram-stain-negative, non-motile, aerobic ovoid- to coccoid-shaped and non-spore-forming. Strain YIM 78023T grew at 20-60 ºC and pH 6.0-9.0 with optimal growth observed at 40-50 ºC and pH 8.0, while strain YIM 78058 grew at 25-60 ºC and pH 6.0-10.0 with optimal growth at 45-50 ºC and pH 8.0. Phylogenetic analysis based on 16S rRNA gene sequences affiliated these two isolates within the family Acetobacteraceae with high sequence similarities to members of the genera Roseomonas and Belnapia (all sequence similarities <94.5 %). In addition to the above two genera, these strains also clustered with the genera Craurococcus and Paracraurococcus (having sequence similarities <93.3 %) in the phylogenetic tree, but with a distinct lineage within the family Acetobacteraceae. The major ubiquinone was Q-10 and the major fatty acids observed were C18:1ω7c, summed feature 4 and C16:0. The genomic DNA G+C contents observed for strains YIM 78023T and YIM 78058 were 74.3 and 74.0 mol%, respectively. Morphological, phylogenetic and chemotaxonomic results suggest that strains YIM 78023T and YIM 78058 are representatives of a novel species of a new genus within the family Acetobacteraceae, for which the name Crenalkalicoccus roseus gen. nov., sp. nov. is proposed. The type strain of Crenalkalicoccus roseus is YIM 78023T (=JCM 19657T=KACC 17825T).
authors.affiliation: College of Life Sciences and Technology, Xinxiang Medical University, Xinxiang 453003, PR China.|Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Ming
authors.affiliation: College of Fisheries, Henan Normal University, Xinxiang 453007, PR China.
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Duan
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Yi-Rui
authors.initials: YR
authors.lastname: Yin
authors.affiliation: College of Fisheries, Henan Normal University, Xinxiang 453007, PR China.
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Meng
authors.affiliation: College of Fisheries, Henan Normal University, Xinxiang 453007, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Li
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.|State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
authors.forename: En-Min
authors.initials: EM
authors.lastname: Zhou
authors.affiliation: College of Fisheries, Henan Normal University, Xinxiang 453007, PR China.
authors.forename: Jian-Rong
authors.initials: JR
authors.lastname: Huang
authors.affiliation: College of Fisheries, Henan Normal University, Xinxiang 453007, PR China.
authors.forename: Guo-Xing
authors.initials: GX
authors.lastname: Nie
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.|Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology; D045482:Hot Springs / Q000382:microbiology*; D042082:Methylobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C024989:coenzyme Q10