title: [ThE COMPARATIVE CHARACTERISTIC OF PHENOTYPIC AND GENOTYPIC RESISTANCE TO AMINOGLYCOSIDES OF STRAINS STAPHYLOCOCCUS AUREUS ISOLATED IN TRAUMATOLOGICAI ORTHOPEDIC HOSPITAL].
abstract: The clinical isolates of Staphylococcus aureus (n = 102) were analyzed on sensitivity and to gentamicin, tobramicin, netimicin and amikacin. The disc diffusing technique was applied. The technique ofpolymerase chain reaction was applied to analyze all strains establishing presence in their genomes genes aac (6'-Ie/aph(2"), ant1, aac, ant(6)-Ia, aph (3')-IIIa and ant(4')-Ia coding amino-glycoside-modifying enzymes. The strains sensitive to amino-glycosides had no the given genes in genome. The genome of all strains resistant to amino-glycosides included no less than two of enumerated genes. The 100% correlation was established between phenotypic resistance of analyzed strains to amino-glycosides and availability in them of gene aac(6')-Ie/aph(2").
authors.forename: E M
authors.initials: EM
authors.lastname: Poliakova
authors.forename: S A
authors.initials: SA
authors.lastname: Bojkova
mesh_terms: D000123:Acetyltransferases / Q000235:genetics* / Q000378:metabolism; D000583:Amikacin / Q000378:metabolism / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D001711:Biotransformation; D052978:Disk Diffusion Antimicrobial Tests; D024881:Drug Resistance, Bacterial / Q000235:genetics; D018628:Gene Dosage; D015870:Gene Expression; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D005838:Genotype; D005839:Gentamicins / Q000378:metabolism / Q000494:pharmacology; D006761:Hospitals; D006801:Humans; D009428:Netilmicin / Q000378:metabolism / Q000494:pharmacology; D009985:Orthopedics; D010641:Phenotype; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics* / Q000378:metabolism; D012426:Russia; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology* / Q000473:pathology / Q000601:surgery; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D014031:Tobramycin / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D009428:Netilmicin; D000583:Amikacin; C108453:6'-aminoglycoside acetyltransferase-2''-aminoglycoside phosphotransferase; D000123:Acetyltransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); D014031:Tobramycin