title: Nanografted Substrata and Triculture of Human Pericytes, Fibroblasts, and Endothelial Cells for Studying the Effects on Angiogenesis.
abstract: For the successful treatment of severe wounds, angiogenesis must be induced to provide a sufficient blood supply to the wound site. There have been many studies on various structural and biochemical factors regulating angiogenesis, such as surface topography, surface modifications, angiogenic factors, and various cell types. However, there is a paucity of research on the effects of micro- and nanoscale topography and the application of pericytes in angiogenesis. In this study, we utilized nanoscale topography combined with a triculture system consisting of human pericytes, fibroblasts, and endothelial cells. We investigated the effects of the nanografted substrata and a triculture system on proangiogenic therapies in vitro. Human dermal fibroblasts and human umbilical vein endothelial cells were seeded with human pericytes from the placenta directly onto nanografted substrata composed of polydimethylsiloxane. We demonstrated that key elements of angiogenesis, including segment and capillary-like tubular structure formation, as well as the secretion of extracellular matrix, were increased by interaction between the nanografted substrata and the coculture containing pericytes. Thus, nanografted surfaces and triculture systems containing human pericytes, fibroblasts, and endothelial cells promote angiogenesis.
authors.affiliation: 1 Biomaterials Research Center, Korea Institute of Science and Technology , Seoul, Republic of Korea .|2 NBIT, KU-KIST Graduate School of Converging Science and Technology, Korea University , Seoul, Republic of Korea .
authors.forename: Tae Hee
authors.initials: TH
authors.lastname: Kim
authors.affiliation: 1 Biomaterials Research Center, Korea Institute of Science and Technology , Seoul, Republic of Korea .|2 NBIT, KU-KIST Graduate School of Converging Science and Technology, Korea University , Seoul, Republic of Korea .|3 Department of Biomedical Engineering, Korea University of Science and Technology (UST), Daejeon, Republic of Korea .
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Kim
authors.affiliation: 4 Department of Biomedical Engineering, Columbia University , New York, New York.
authors.forename: Kam W
authors.initials: KW
authors.lastname: Leong
authors.affiliation: 1 Biomaterials Research Center, Korea Institute of Science and Technology , Seoul, Republic of Korea .|3 Department of Biomedical Engineering, Korea University of Science and Technology (UST), Daejeon, Republic of Korea .
authors.forename: Youngmee
authors.initials: Y
authors.lastname: Jung
mesh_terms: D018920:Coculture Techniques; D004129:Dimethylpolysiloxanes / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D018919:Neovascularization, Physiologic / Q000187:drug effects; D020286:Pericytes / Q000166:cytology* / Q000187:drug effects; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism
chemical_list: D004129:Dimethylpolysiloxanes; D019408:Platelet Endothelial Cell Adhesion Molecule-1; C013830:baysilon