title: Proteomics-Based Analysis of Protein Complexes in Pluripotent Stem Cells and Cancer Biology.
abstract: A protein complex consists of two or more proteins that are linked together through protein-protein interactions. The proteins show stable/transient and direct/indirect interactions within the protein complex or between the protein complexes. Protein complexes are involved in regulation of most of the cellular processes and molecular functions. The delineation of protein complexes is important to expand our knowledge on proteins functional roles in physiological and pathological conditions. The genetic yeast-2-hybrid method has been extensively used to characterize protein-protein interactions. Alternatively, a biochemical-based affinity purification coupled with mass spectrometry (AP-MS) approach has been widely used to characterize the protein complexes. In the AP-MS method, a protein complex of a target protein of interest is purified using a specific antibody or an affinity tag (e.g., DYKDDDDK peptide (FLAG) and polyhistidine (His)) and is subsequently analyzed by means of MS. Tandem affinity purification, a two-step purification system, coupled with MS has been widely used mainly to reduce the contaminants. We review here a general principle for AP-MS-based characterization of protein complexes and we explore several protein complexes identified in pluripotent stem cell biology and cancer biology as examples.
authors.affiliation: Genomics Research Center, Academia Sinica, Taipei 11529, Taiwan. preddy@gate.sinica.edu.tw.
authors.forename: Putty-Reddy
authors.initials: PR
authors.lastname: Sudhir
authors.affiliation: Genomics Research Center, Academia Sinica, Taipei 11529, Taiwan. winschen@gate.sinica.edu.tw.
authors.forename: Chung-Hsuan
authors.initials: CH
authors.lastname: Chen
mesh_terms: D000818:Animals; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D046912:Multiprotein Complexes / Q000737:chemistry* / Q000378:metabolism; D009369:Neoplasms / Q000378:metabolism*; D039904:Pluripotent Stem Cells / Q000378:metabolism*; D055503:Protein Multimerization; D040901:Proteomics / Q000379:methods*
chemical_list: D046912:Multiprotein Complexes
keywords: affinity purification; cancer biology; mass spectrometry; pluripotent stem cells; protein complexes; proteomics