title: Simultaneous determination of shanzhiside methyl ester, 8-O-acetylshan- zhiside methyl ester and luteolin-7-O-β-D-glucopyranoside in rat plasma by ultra performance liquid chromatography-tandem mass spectrometry and its application to a pharmacokinetic study after oral administration of Lamiophlomis rotata Pill.
abstract: A rapid, sensitive and specific ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the quantification of shanzhiside methyl ester, 8-O-acetylshanzhiside methyl ester and luteolin-7-O-β-D-glucopyranoside of Lamiophlomis rotata Pill in rat plasma was developed and validated. After liquid-liquid extraction with n-butyl alcohol/ethyl acetate (70:30, v/v), analytes and paeoniflorin (internal standard, IS) were separated on an Acquity BEH UPLC C18 column (100 × 2.1 mm, 1.7 μm) with gradient elution at a flow rate of 0.2 mL/min. All calibration curves had good linearity (r>0.9929) over the concentration ranges of 1-1000 ng/mL for shanzhiside methyl ester and 8-O-acetylshanzhiside methyl ester, 0.3-150 ng/mL for luteolin-7-O-β-D-glucopyranoside. The intra- and inter-day precisions were all within 11.1% and the accuracy (relative error, RE%) all ranged from -13.6% to 5.3%. The method also guaranteed an acceptable selectivity, recovery and stability, which was successfully applied to a pharmacokinetic study of the three analytes in rats after oral administration of Lamiophlomis rotata Pill.
authors.affiliation: School of Pharmacy, Shenyang Pharmaecutical University, Shenyang 110016, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: School of Pharmacy, Shenyang Pharmaecutical University, Shenyang 110016, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Shenyang Pharmaecutical University, Shenyang 110016, China.
authors.forename: Xinlei
authors.initials: X
authors.lastname: Liang
authors.affiliation: School of Pharmacy, Shenyang Pharmaecutical University, Shenyang 110016, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Sun
authors.affiliation: School of Pharmacy, Shenyang Pharmaecutical University, Shenyang 110016, China.
authors.forename: Jinghan
authors.initials: J
authors.lastname: Luo
authors.affiliation: School of Pharmacy, Shenyang Pharmaecutical University, Shenyang 110016, China.
authors.forename: Xingjie
authors.initials: X
authors.lastname: Guo
authors.affiliation: School of Pharmacy, Shenyang Pharmaecutical University, Shenyang 110016, China. Electronic address: longshanzhao@163.com.
authors.forename: Longshan
authors.initials: L
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics*; D005960:Glucosides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D016014:Linear Models; D047311:Luteolin / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D005960:Glucosides; C066408:luteolin-7-glucoside; D047311:Luteolin
keywords: Lamiophlomis rotata pill; Pharmacokinetics; Rat plasma; UPLC–MS/MS