title: Genomic, pathogenic, and antigenic comparisons of Getah virus strains isolated in 1978 and 2014 in Japan.
abstract: A Getah virus strain isolated during an outbreak in racehorses in Japan in 2014 (14-I-605) was compared with the vaccine strain isolated in 1978 (MI-110). A comparison of the genome sequences of these strains revealed seven amino acid substitutions in non-structural protein 3, and one or two substitutions in each of other non-structural proteins. In contrast, the structural proteins were highly conserved (99.8-99.9 % amino acid sequence identity). Horse antisera raised against the MI-110 strain showed similar virus-neutralization titers against both MI-110 and 14-I-605 strains (512 and 256, respectively). Therefore, antigenic mutation was probably not a direct cause of the outbreak that occurred in 2014.
authors.affiliation: Equine Research Institute, Japan Racing Association, 1400-4, Shiba, Shimotsuke, Tochigi, 329-0412, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Nemoto
authors.affiliation: Equine Research Institute, Japan Racing Association, 1400-4, Shiba, Shimotsuke, Tochigi, 329-0412, Japan. bannai@equinst.go.jp.
authors.forename: Hiroshi
authors.identifier: http://orcid.org/0000-0002-9573-5901
authors.initials: H
authors.lastname: Bannai
authors.affiliation: Equine Research Institute, Japan Racing Association, 1400-4, Shiba, Shimotsuke, Tochigi, 329-0412, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Tsujimura
authors.affiliation: Equine Research Institute, Japan Racing Association, 1400-4, Shiba, Shimotsuke, Tochigi, 329-0412, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamanaka
authors.affiliation: Equine Research Institute, Japan Racing Association, 1400-4, Shiba, Shimotsuke, Tochigi, 329-0412, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondo
mesh_terms: D000524:Alphavirus / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D018354:Alphavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D019943:Amino Acid Substitution; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics; D003429:Cross Reactions; D004196:Disease Outbreaks / Q000662:veterinary; D016679:Genome, Viral; D006734:Horse Diseases / Q000453:epidemiology / Q000821:virology*; D006736:Horses; D007564:Japan / Q000453:epidemiology; D010802:Phylogeny; D013997:Time Factors; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000956:Antigens, Viral; D014764:Viral Proteins