title: Sustainable efficient way for opioid peptide LVV-h7 preparation from enzymatic proteolysis in a microfluidic-based reaction-extraction process with solvent recycling.
abstract: LVV-h7 (LVVYPWTQFR) is a bioactive peptide that can be obtained from blood as waste of food industry, more precisely from hemoglobin hydrolysis by pepsin. This opioid peptide belongs to the hemorphins family and have strong physiological effects that bring its use in pharmaceutics and various therapeutic treatments attractive, in particular for substituting its costly chemically synthetized analogous. Hemoglobin hydrolysis by pepsin generates a huge variety of peptides among whose LVV-h7 can be purified by liquid-liquid extraction (LLE). Herein, selective preparation of this peptide is proposed by a microfluidic-based continuous reaction-separation process. Hemoglobin hydrolysis in microreactor was firstly coupled to LVV-h7 LLE in octan-1-ol and then coupled to LVV-h7 back LLE in acidic water. This continuous process allowed to prepare pure LVV-h7, as confirmed by liquid chromatography and mass spectrometry. The microfluidic circuit also allowed octan-1-ol recycling in a closed loop, making this method more sustainable than similar biphasic batch process.
authors.affiliation: Univ. Lille, EA 7394, USC 1281, ICV-Institut Charles Viollette, F-59655 Villeneuve d'Ascq, France; Institut d'Electronique, de Microélectronique et de Nanotechnologie (IEMN)-UMR CNRS 8520, Université Lille 1-Sciences et Technologies, Villeneuve d'Ascq, France.
authors.forename: Adil
authors.initials: A
authors.lastname: Elagli
authors.affiliation: Univ. Lille, EA 7394, USC 1281, ICV-Institut Charles Viollette, F-59655 Villeneuve d'Ascq, France; Institut d'Electronique, de Microélectronique et de Nanotechnologie (IEMN)-UMR CNRS 8520, Université Lille 1-Sciences et Technologies, Villeneuve d'Ascq, France.
authors.forename: Kalim
authors.initials: K
authors.lastname: Belhacene
authors.affiliation: Univ. Lille, EA 7394, USC 1281, ICV-Institut Charles Viollette, F-59655 Villeneuve d'Ascq, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Dhulster
authors.affiliation: Univ. Lille, EA 7394, USC 1281, ICV-Institut Charles Viollette, F-59655 Villeneuve d'Ascq, France. Electronic address: renato.froidevaux@univ-lille1.fr.
authors.forename: Renato
authors.initials: R
authors.lastname: Froidevaux
mesh_terms: D000818:Animals; D019149:Bioreactors; D002417:Cattle; D004867:Equipment Design; D017743:Equipment Reuse; D006454:Hemoglobins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D059625:Liquid-Liquid Extraction / Q000295:instrumentation* / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D008954:Models, Biological; D010434:Pepsin A / Q000737:chemistry / Q000378:metabolism*; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D059748:Proteolysis
chemical_list: D006454:Hemoglobins; D010446:Peptide Fragments; C074309:LVV-hemorphin-7; D010434:Pepsin A
keywords: Bioactive peptide; Enzymatic hydrolysis; Liquid–liquid extraction; Microfluidics; Solvent recycling