title: Intraperitoneal Injection of Multiplacentas Pooled Cells Treatment on a Mouse Model with Aplastic Anemia.
abstract: Coinfusion of hematopoietic and mesenchymal stem cells is more effective than hematopoietic stem cell transplantation alone. It is necessary to explore a safe and routine mixed stem cell intraperitoneal transplantation method. Multiplacentas pooled cells were intraperitoneally injected into a radiation- and immunity-induced mouse aplastic anemia model with single time. Then, mouse survival time, peripheral blood hemoglobin count, bone marrow architecture, and donor cell engraftment were assessed. The recipient mouse exhibited donor cell engraftment in both bone marrow and peripheral blood. Survival time and peripheral blood hemoglobin count increased in placenta pooled cells treated mice, compared with model-only controls (P = 0.048 and P = 0.000, resp.). However, placentas pooled cells failed to cause a significant decrease in bone marrow pimelosis area (P = 0.357). Intraperitoneally transplanted multiplacentas pooled cells can survive and engraft into a host body through blood circulation, which can increase the life span of an aplastic anemia model mice, and delay but not abrogate the development of aplastic anemia. Furthermore, they appear to play a role in increasing peripheral blood hemoglobin level response for increasing the life span of aplastic anemia model mice.
authors.affiliation: Medical School, Kunming University, Kunming 650214, China; Stem Cell, Tissue and Organ Engineering Research Center, Kunming General Hospital of Chengdu Military Command, Kunming 650032, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Oncology, Kunming General Hospital of Chengdu Military Command, Kunming 650032, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.affiliation: Stem Cell, Tissue and Organ Engineering Research Center, Kunming General Hospital of Chengdu Military Command, Kunming 650032, China.
authors.forename: Yan-Bo
authors.initials: YB
authors.lastname: Lv
authors.affiliation: Stem Cell, Tissue and Organ Engineering Research Center, Kunming General Hospital of Chengdu Military Command, Kunming 650032, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Haematology and Endocrinology, Kunming General Hospital of Chengdu Military Command, Kunming 650032, China.
authors.forename: Zheng-Jun
authors.initials: ZJ
authors.lastname: Xie
authors.affiliation: Stem Cell, Tissue and Organ Engineering Research Center, Kunming General Hospital of Chengdu Military Command, Kunming 650032, China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Ma
authors.affiliation: Stem Cell, Tissue and Organ Engineering Research Center, Kunming General Hospital of Chengdu Military Command, Kunming 650032, China.
authors.forename: Jie
authors.initials: J
authors.lastname: He
authors.affiliation: Medical School, Kunming University, Kunming 650214, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xue
authors.affiliation: Medical School, Kunming University, Kunming 650214, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Yu
authors.affiliation: Digestive System Department, Kunming General Hospital of Chengdu Military Command, Kunming 650032, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Guo
authors.affiliation: Neuroscience Institute of Kunming Medical University, Kunming 650500, China.
authors.forename: Ting-Hua
authors.initials: TH
authors.lastname: Wang
authors.affiliation: Medical School, Kunming University, Kunming 650214, China.
authors.forename: Tian-Xi
authors.initials: TX
authors.lastname: Wu
authors.affiliation: Stem Cell, Tissue and Organ Engineering Research Center, Kunming General Hospital of Chengdu Military Command, Kunming 650032, China.
authors.forename: Xing-Hua
authors.initials: XH
authors.lastname: Pan