title: Synthesis and structural insight into ESX-1 Substrate Protein C, an immunodominant Mycobacterium tuberculosis-secreted antigen.
abstract: Tuberculosis, the second leading cause of death from a single infectious agent, is recognized as a major threat to human health due to a lack of practicable vaccines against the disease and the widespread occurrence of drug resistance. With a pressing need for a novel protein target as a platform for new vaccine development, ESX-1 Substrate Protein C (EspC) was recently identified as a novel Mycobacterium tuberculosis-secreted antigen that is as immunodominant as the two specific immunodiagnostic T-cell antigens, CFP-10 and ESAT-6. Here, we present the first chemical total synthesis, folding conditions, and circular dichroism data of EspC. © 2016 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 106: 267-274, 2016.
authors.affiliation: School of Biological Sciences, The University of Auckland, 3a Symonds Street, Auckland, 1010, New Zealand.
authors.forename: Soo Jung
authors.initials: SJ
authors.lastname: Son
authors.affiliation: School of Biological Sciences, The University of Auckland, 3a Symonds Street, Auckland, 1010, New Zealand.|School of Chemical Sciences, The University of Auckland, 23 Symonds Street, Auckland, 1010, New Zealand.|Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland, 3a Symonds Street, Auckland, 1010, New Zealand.
authors.forename: Paul W R
authors.initials: PW
authors.lastname: Harris
authors.affiliation: School of Biological Sciences, The University of Auckland, 3a Symonds Street, Auckland, 1010, New Zealand.|Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland, 3a Symonds Street, Auckland, 1010, New Zealand.
authors.forename: Chris J
authors.initials: CJ
authors.lastname: Squire
authors.affiliation: School of Biological Sciences, The University of Auckland, 3a Symonds Street, Auckland, 1010, New Zealand.|Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland, 3a Symonds Street, Auckland, 1010, New Zealand.
authors.forename: Edward N
authors.initials: EN
authors.lastname: Baker
authors.affiliation: School of Biological Sciences, The University of Auckland, 3a Symonds Street, Auckland, 1010, New Zealand.|School of Chemical Sciences, The University of Auckland, 23 Symonds Street, Auckland, 1010, New Zealand.|Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland, 3a Symonds Street, Auckland, 1010, New Zealand.
authors.forename: Margaret A
authors.initials: MA
authors.lastname: Brimble
mesh_terms: D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000737:chemistry / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D015870:Gene Expression; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000737:chemistry* / Q000276:immunology; D010455:Peptides / Q000138:chemical synthesis* / Q000276:immunology; D017510:Protein Folding; D017433:Protein Structure, Secondary; D012996:Solutions; D000069377:Type VII Secretion Systems / Q000235:genetics
chemical_list: D000942:Antigens, Bacterial; D000945:Antigens, Differentiation, T-Lymphocyte; D001426:Bacterial Proteins; C093276:ESAT-6 protein, Mycobacterium tuberculosis; D010455:Peptides; D012996:Solutions; D000069377:Type VII Secretion Systems
keywords: SPPS; antigen; mycobacterium tuberculosis; native chemical ligation