title: Localizing PRL-2 expression and determining the effects of dietary Mg(2+) on expression levels.
abstract: The phosphatase of regenerating liver (PRL) is a group of protein tyrosine phosphatases that play a key role in cancer progression and metastasis. We previously showed that PRL-2 modulates intracellular Mg(2+) levels and sustains cancer phenotypes by binding to the Mg(2+) transporter CNNM3. However, the physiological functions of PRL-2 in animals remain largely unknown. To better understand which cell types are associated with PRL-2 function, we characterized its expression in mouse tissues using a PRL-2 β-galactosidase reporter mouse model. Our results demonstrated that PRL-2 was ubiquitously expressed, with the highest expression levels observed in the hippocampal pyramidal neurons, ependymal cells, cone and rod photoreceptor cells, endocardium, vascular and bronchial smooth muscle, and collecting ducts in the kidney. On the other hand, PRL-2 expression was undetectable or very low in the parenchymal cells of the liver and pancreas. Our results also indicated that PRL-2 is involved in cell-type-specific Mg(2+) homeostasis and that PRL-2 expression is potentially inversely regulated by dietary Mg(2+) levels.
authors.affiliation: Department of Microbiology and Immunology, 3775 University Street, Montreal, QC, H3A 2B4, Canada.
authors.forename: Jeremy
authors.initials: J
authors.lastname: Gungabeesoon
authors.affiliation: Department of Microbiology and Immunology, 3775 University Street, Montreal, QC, H3A 2B4, Canada.|Department of Biochemistry, McGill University, 3655 Promenade Sir William Osler, Montreal, QC, H3G 1Y6, Canada.|Rosalind and Morris Goodman Cancer Research Centre, McGill University, Cancer Research Building, 1160 Pine Avenue West, Room 618, Montreal, QC, H3A 1A3, Canada.
authors.forename: Michel L
authors.initials: ML
authors.lastname: Tremblay
authors.affiliation: Rosalind and Morris Goodman Cancer Research Centre, McGill University, Cancer Research Building, 1160 Pine Avenue West, Room 618, Montreal, QC, H3A 1A3, Canada. noriko.uetani@mcgill.ca.
authors.forename: Noriko
authors.initials: N
authors.lastname: Uetani
mesh_terms: D000818:Animals; D019587:Dietary Supplements; D005260:Female; D006706:Homeostasis / Q000187:drug effects; D017874:Immediate-Early Proteins / Q000032:analysis* / Q000096:biosynthesis* / Q000378:metabolism; D008274:Magnesium / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D017027:Protein Tyrosine Phosphatases / Q000032:analysis* / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D017874:Immediate-Early Proteins; D017027:Protein Tyrosine Phosphatases; C111533:Ptp4a2 protein, mouse; D008274:Magnesium
keywords: CNNM; Magnesium homeostasis; PRL-2; PTP4A2; Tyrosine phosphatase