title: Solution structure of TbTFIIS2-1 PWWP domain from Trypanosoma brucei.
abstract: TbTFIIS2-1, one of the two TFIIS homologues of Trypanosome brucei (T. brucei), cooperates with TbTFIIS1 in regulating transcription in T. brcuei. Structurally divergent from other TFIIS homologues from higher organisms, TbTFIIS2-1 contains an additional N-terminal PWWP domain besides other three conserved domains, which may imply potential role of TbTFIIS2-1 in transcription regulation. Here, we determined the solution structure of PWWP domain of TbTFIIS2-1 by NMR spectroscopy, which was the first solution structure of PWWP domain solved in trypanosomatid. In spite of poor sequence similarity between PWWP domains, this domain of TbTFIIS2-1 adopts a conserved 3D-structure, which contains a five-stranded β-barrel and a C-terminal α-helix. Furthermore, we found that TbTFIIS2-1 PWWP domain may be a protein-protein interaction module without the ability of DNA recognition and methyl-group interaction. Proteins 2016; 84:912-919. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale, and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, 230026, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale, and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, 230026, People's Republic of China.
authors.forename: Jiahai
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale, and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, 230026, People's Republic of China.
authors.forename: Shanhui
authors.initials: S
authors.lastname: Liao
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale, and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, 230026, People's Republic of China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Tu
mesh_terms: D000595:Amino Acid Sequence; D006801:Humans; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D000072417:Protein Domains; D015800:Protozoan Proteins / Q000737:chemistry*; D035602:Transcriptional Elongation Factors / Q000737:chemistry*; D014346:Trypanosoma brucei brucei / Q000737:chemistry*; D014353:Trypanosomiasis, African / Q000469:parasitology
chemical_list: D015800:Protozoan Proteins; D035602:Transcriptional Elongation Factors; C018648:transcription factor S-II
keywords: NMR; T. brucei; TbTFIIS2-1 PWWP domain