title: Substrate channelling as an approach to cascade reactions.
abstract: Millions of years of evolution have produced biological systems capable of efficient one-pot multi-step catalysis. The underlying mechanisms that facilitate these reaction processes are increasingly providing inspiration in synthetic chemistry. Substrate channelling, where intermediates between enzymatic steps are not in equilibrium with the bulk solution, enables increased efficiencies and yields in reaction and diffusion processes. Here, we review different mechanisms of substrate channelling found in nature and provide an overview of the analytical methods used to quantify these effects. The incorporation of substrate channelling into synthetic cascades is a rapidly developing concept, and recent examples of the fabrication of cascades with controlled diffusion and flux of intermediates are presented.
authors.affiliation: Chemical and Environmental Engineering, University of California Riverside, California 92521, USA.
authors.forename: Ian
authors.initials: I
authors.lastname: Wheeldon
authors.affiliation: Chemistry, University of Utah, Utah 84112, USA.
authors.forename: Shelley D
authors.initials: SD
authors.lastname: Minteer
authors.affiliation: Chemical Engineering, Columbia University, New York 10027, USA.
authors.forename: Scott
authors.initials: S
authors.lastname: Banta
authors.affiliation: Chemical and Materials Engineering, Michigan State University, Michigan 48824, USA.
authors.forename: Scott Calabrese
authors.initials: SC
authors.lastname: Barton
authors.affiliation: Department of Chemical and Biological Engineering and Center for Micro-Engineered Materials, University of New Mexico, New Mexico 87131, USA.
authors.forename: Plamen
authors.initials: P
authors.lastname: Atanassov
authors.affiliation: Chemistry, University of Utah, Utah 84112, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Sigman
mesh_terms: D055162:Biocatalysis; D020134:Catalytic Domain; D004058:Diffusion; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004798:Enzymes