title: Chlorogenic acid from honeysuckle improves hepatic lipid dysregulation and modulates hepatic fatty acid composition in rats with chronic endotoxin infusion.
abstract: Chlorogenic acid as a natural hydroxycinnamic acid has protective effect for liver. Endotoxin induced metabolic disorder, such as lipid dysregulation and hyperlipidemia. In this study, we investigated the effect of chlorogenic acid in rats with chronic endotoxin infusion. The Sprague-Dawley rats with lipid metabolic disorder (LD group) were intraperitoneally injected endotoxin. And the rats of chlorogenic acid-LD group were daily received chlorogenic acid by intragastric administration. In chlorogenic acid-LD group, the area of visceral adipocyte was decreased and liver injury was ameliorated, as compared to LD group. In chlorogenic acid-LD group, serum triglycerides, free fatty acids, hepatic triglycerides and cholesterol were decreased, the proportion of C20:1, C24:1 and C18:3n-6, Δ9-18 and Δ6-desaturase activity index in the liver were decreased, and the proportion of C18:3n-3 acid was increased, compared to the LD group. Moreover, levels of phosphorylated AMP-activated protein kinase, carnitine palmitoyltransferase-I, and fatty acid β-oxidation were increased in chlorogenic acid-LD group compared to LD rats, whereas levels of fatty acid synthase and acetyl-CoA carboxylase were decreased. These findings demonstrate that chlorogenic acid effectively improves hepatic lipid dysregulation in rats by regulating fatty acid metabolism enzymes, stimulating AMP-activated protein kinase activation, and modulating levels of hepatic fatty acids.
authors.affiliation: State Key Laboratory of Food Science and Technology and College of Food Science, Nanchang University, Nanchang 330047, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Food Science and Technology and College of Food Science, Nanchang University, Nanchang 330047, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Ruan
authors.affiliation: State Key Laboratory of Food Science and Technology and College of Food Science, Nanchang University, Nanchang 330047, China.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Wen
authors.affiliation: State Key Laboratory of Food Science and Technology and College of Food Science, Nanchang University, Nanchang 330047, China.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Food Science and Technology and College of Food Science, Nanchang University, Nanchang 330047, China.
authors.forename: Shumei
authors.initials: S
authors.lastname: Mi
authors.affiliation: State Key Laboratory of Food Science and Technology and College of Food Science, Nanchang University, Nanchang 330047, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Food Science and Technology and College of Food Science, Nanchang University, Nanchang 330047, China; Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wu
authors.affiliation: Institute of Nutrition and Food Safety, Center for Disease Control and Prevention of Jiangxi Province, Nanchang 330029, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Food Science and Technology and College of Food Science, Nanchang University, Nanchang 330047, China.
authors.forename: Zeyuan
authors.initials: Z
authors.lastname: Deng
authors.affiliation: Department of Animal Science, Faculty of Nutrition, Texas A&M University, College Station 77843-2471, USA.
authors.forename: Guoyao
authors.initials: G
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Food Science and Technology and College of Food Science, Nanchang University, Nanchang 330047, China; Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Yin
keywords: AMP-activated protein kinase; chlorogenic acid; fatty acids; lipid metabolism