title: Detection of ST772 Panton-Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus (Bengal Bay clone) and ST22 S. aureus isolates with a genetic variant of elastin binding protein in Nepal.
abstract: Genetic characteristics were analysed for recent clinical isolates of methicillin-resistant and -susceptible Staphylococcus aureus (MRSA and MSSA respectively) in Kathmandu, Nepal. MRSA isolates harbouring Panton-Valentine leukocidin (PVL) genes were classified into ST1, ST22 and ST88 with SCCmec-IV and ST772 with SCCmec-V (Bengal Bay clone), while PVL-positive MSSA into ST22, ST30 and ST772. ST22 isolates (PVL-positive MRSA and MSSA, PVL-negative MRSA) possessed a variant of elastin binding protein gene (ebpS) with an internal deletion of 180 bp, which was similar to that reported for ST121 S. aureus previously outside Nepal. Phylogenetic analysis indicated that the ebpS variant in ST22 might have occurred independently of ST121 strains. This is the first report of ST772 PVL-positive MRSA in Nepal and detection of the deletion variant of ebpS in ST22 S. aureus.
authors.affiliation: Genesis Laboratory and Research, Tribhuvan University, Kathmandu, Nepal.
authors.forename: R H
authors.initials: RH
authors.lastname: Pokhrel
authors.affiliation: Sapporo Medical University, Sapporo, Japan.
authors.forename: M S
authors.initials: MS
authors.lastname: Aung
authors.affiliation: Genesis Laboratory and Research, Tribhuvan University, Kathmandu, Nepal.
authors.forename: B
authors.initials: B
authors.lastname: Thapa
authors.affiliation: Nepal Army Institute of Health Sciences, Tribhuvan University, Kathmandu, Nepal.
authors.forename: R
authors.initials: R
authors.lastname: Chaudhary
authors.affiliation: Institute of Medicine, Tribhuvan University, Kathmandu, Nepal.
authors.forename: S K
authors.initials: SK
authors.lastname: Mishra
authors.affiliation: Sapporo Medical University, Sapporo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kawaguchiya
authors.affiliation: Sapporo Medical University, Sapporo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Urushibara
authors.affiliation: Sapporo Medical University, Sapporo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Kobayashi
keywords: Bengal Bay clone; MRSA; Nepal; Panton-Valentine leukocidin (PVL); ST22; ST772; elastin binding protein