title: Effect of yeast biomass with high content of carotenoids on erythrocyte deformability, NO production and Na,K-ATPase activity in healthy and LPS treated rats.
abstract: Measurements of red blood cell (RBC) deformability together with estimation of NO-synthase activity and Na,K-ATPase activity were used for characterization of RBC functionality in rats subjected to single dose of Escherichia coli lipopolysaccharides (LPS) at a dose of 1 mg/kg. We hypothesized that LPS might initiate a malfunction of RBC. We also investigated the potential effect of carotenoids (10 mg/kg/day) produced in red yeast biomass of Rhodotorula glutinis on RBC in LPS-challenged rats. LPS significantly reduced the deformability of RBC (by 14%) together with decrease of NO-synthase activity by 20%. Daily supplementation of carotenoids for 10 days attenuated the LPS-induced injury, as observed by 22% increase of RBC deformability and 23% increase of NO-synthase activity. The activity of Na,K-ATPase was also improved probably due to increased number of active enzyme molecules as indicated by 66% enhancement of Vmax value, hence maintaining the activity of erythrocyte Na,K-ATPase to the level even higher as compared with healthy control animals. It may be concluded that administration of yeast biomass with high content of carotenoids resulted in advanced function of erythrocytes as concerns their ability to squeeze through narrow capillaries of the circulation, better intrinsic production of NO and improvement of intracellular homeostasis of sodium.
authors.affiliation: Institute of Physiology, Faculty of Medicine, Comenius University in Bratislava, Bratislava, Slovak Republic.|Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors.forename: J
authors.initials: J
authors.lastname: Radosinska
authors.affiliation: Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors.forename: L
authors.initials: L
authors.lastname: Mezesova
authors.affiliation: Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors.forename: L
authors.initials: L
authors.lastname: Okruhlicova
authors.affiliation: Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors.forename: K
authors.initials: K
authors.lastname: Frimmel
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors.forename: E
authors.initials: E
authors.lastname: Breierova
authors.affiliation: Institute of Physiology, Faculty of Medicine, Comenius University in Bratislava, Bratislava, Slovak Republic.|Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors.forename: M
authors.initials: M
authors.lastname: Bartekova
authors.affiliation: Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors.forename: N
authors.initials: N
authors.lastname: Vrbjar
mesh_terms: D000818:Animals; D018533:Biomass; D002338:Carotenoids / Q000378:metabolism*; D004195:Disease Models, Animal; D004907:Erythrocyte Deformability / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000378:metabolism* / Q000494:pharmacology; D008297:Male; D009569:Nitric Oxide; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D015003:Yeasts
chemical_list: D008070:Lipopolysaccharides; D009569:Nitric Oxide; D002338:Carotenoids; D000254:Sodium-Potassium-Exchanging ATPase
keywords: Erythrocyte deformability; lipopolysaccharide; nitric oxide synthase; sodium pump; yeast carotenoids