title: Antimicrobial peptide (Cn-AMP2) from liquid endosperm of Cocos nucifera forms amyloid-like fibrillar structure.
abstract: Cn-AMP2 is an antimicrobial peptide derived from liquid endosperm of coconut (Cocos nucifera). It consists of 11 amino acid residues and predicted to have high propensity for β-sheet formation that disposes this peptide to be amyloidogenic. In the present study, we have examined the amyloidogenic propensities of Cn-AMP2 in silico and then tested the predictions under in vitro conditions. The in silico study revealed that the peptide possesses high amyloidogenic propensity comparable with Aβ. Upon solubilisation and agitation in aqueous buffer, Cn-AMP2 forms visible aggregates that display bathochromic shift in the Congo red absorbance spectra, strong increase in thioflavin T fluorescence and fibrillar morphology under transmission electron microscopy. All these properties are typical of an amyloid fibril derived from various proteins/peptides including Aβ.
authors.affiliation: Department of Biotechnology, Central University of Rajasthan, Bandersindri NH-8 Jaipur-Ajmer Highway, Dist., Ajmer, 305 801, Rajasthan, India.
authors.forename: Shalini
authors.initials: S
authors.lastname: Gour
authors.affiliation: Department of Biotechnology, Central University of Rajasthan, Bandersindri NH-8 Jaipur-Ajmer Highway, Dist., Ajmer, 305 801, Rajasthan, India.
authors.forename: Vibha
authors.initials: V
authors.lastname: Kaushik
authors.affiliation: Department of Biotechnology, Central University of Rajasthan, Bandersindri NH-8 Jaipur-Ajmer Highway, Dist., Ajmer, 305 801, Rajasthan, India.
authors.forename: Vijay
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Department of Biotechnology, Central University of Rajasthan, Bandersindri NH-8 Jaipur-Ajmer Highway, Dist., Ajmer, 305 801, Rajasthan, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Bhat
authors.affiliation: Department of Anatomy, All India Institute of Medical Sciences, New Delhi, -110029, India.
authors.forename: Subhash C
authors.initials: SC
authors.lastname: Yadav
authors.affiliation: Department of Biotechnology, Central University of Rajasthan, Bandersindri NH-8 Jaipur-Ajmer Highway, Dist., Ajmer, 305 801, Rajasthan, India.
authors.forename: Jay K
authors.initials: JK
authors.lastname: Yadav
mesh_terms: D000682:Amyloid / Q000737:chemistry / Q000648:ultrastructure; D000890:Anti-Infective Agents / Q000737:chemistry; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry*; D003059:Cocos / Q000737:chemistry*; D056625:Endosperm / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D066329:Protein Aggregates; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary
chemical_list: D000682:Amyloid; D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D010940:Plant Proteins; D066329:Protein Aggregates
keywords: amyloids; antimicrobial peptides (AMPs); cross β structure; oligomers