title: Taxifolin and Fucoidin Abolish the Irradiation-Induced Increase in the Production of Reactive Oxygen Species in Rat Aorta.
abstract: We studied changes in ROS content in the aorta of Wistar rats at early terms after irradiation in doses equal to single fraction used in tumor radiotherapy and the effects of taxifolin and fucoidin, blockers of leukocyte adhesion to endothelium, on ROS content. Male rats were exposed to X-rays (200 kW) in doses of 1-7.5 Gy. ROS production in aorta segments was measured in 1-48 h after irradiation by dichlorodihydrofluorescein oxidation. The content of ROS in the aorta of rats exposed to radiation in doses of 1-2.5 Gy increased in 1-24 h after irradiation, the peak ROS content was found in 2 h after irradiation. Taxifolin (100 μg/kg dihydroquercetin once a day with drinking water) and fucoidin (10 mg/kg, i.v.) abolished ROS accumulation. The content of ROS in rat aorta increased in 1-24 h after irradiation in doses used for tumor radiotherapy and this increase can be determined by leukocyte adhesion to the endothelium.
authors.affiliation: Laboratory of Oxidative Stress, Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
authors.forename: T V
authors.initials: TV
authors.lastname: Arutyunyan
authors.affiliation: Laboratory of Oxidative Stress, Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
authors.forename: A F
authors.initials: AF
authors.lastname: Korystova
authors.affiliation: Laboratory of Oxidative Stress, Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
authors.forename: L N
authors.initials: LN
authors.lastname: Kublik
authors.affiliation: Laboratory of Oxidative Stress, Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
authors.forename: M Kh
authors.initials: MKh
authors.lastname: Levitman
authors.affiliation: Laboratory of Oxidative Stress, Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Shaposhnikova
authors.affiliation: Laboratory of Oxidative Stress, Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia. ykorystov@rambler.ru.
authors.forename: Yu N
authors.initials: YN
authors.lastname: Korystov
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism / Q000528:radiation effects*; D002448:Cell Adhesion / Q000502:physiology / Q000528:radiation effects*; D004727:Endothelium / Q000378:metabolism; D007962:Leukocytes / Q000502:physiology / Q000528:radiation effects; D008297:Male; D011134:Polysaccharides / Q000494:pharmacology*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism / Q000528:radiation effects*; D014965:X-Rays
chemical_list: D011134:Polysaccharides; D017382:Reactive Oxygen Species; C007789:fucoidan; D011794:Quercetin; C003377:taxifolin
keywords: aorta; fucoidin; irradiation; reactive forms of oxygen; taxifolin