title: Steroidal saponins from Chlorophytum deistelianum.
abstract: Phytochemical investigation of the aerial parts of Chlorophytum deistelianum led to the isolation of four previously undescribed steroidal saponins called chlorodeistelianosides A-D with five known ones. Their structures were established mainly by extensive 1D and 2D NMR spectroscopic techniques and mass spectrometry as (25R)-3β-[(β-D-glucopyranosyl-(1→3)-[α-L-rhamnopyranosyl-(1→4)]-β-D-xylopyranosyl-(1→3)-[β-D-glucopyranosyl-(1→2)]-β-D-glucopyranosyl-(1→4)-β-D-galactopyranosyl)oxy]-5α-spirostan-12-one, (24S,25S)-24-[(β-D-glucopyranosyl)oxy]-3β-[(β-d-glucopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucopyranosyl-(1→4)-β-D-galactopyranosyl)oxy]-5α-spirostan-12-one, (25R)-26-[(β-D-glucopyranosyl)oxy]-2α-hydroxy-22α-methoxy-5α-furostan-3β-yl β-D-glucopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside, and (25R)-26-[(β-D-glucopyranosyl)oxy]-3β-[(β-D-glucopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucopyranosyl-(1→4)-β-D-galactopyranosyl)oxy]-5α-furost-20(22)-en-12-one. Cytotoxicity of most compounds was evaluated against one human cancer cell line (SW480) and one rat cardiomyoblast cell line (H9c2). Among them, three known spirostane-type glycosides exhibited cytotoxicity on both cell lines with IC50 ranging from 8 to 10 μM.
authors.affiliation: Laboratoire de Pharmacognosie, EA 4267/UFC, UFR des Sciences de Santé, Université de Bourgogne Franche-Comté, 7, Bd. Jeanne d'Arc, BP 87900, 21079 Dijon Cedex, France; Département de Chimie Organique, Université de Yaoundé 1, BP 812 Yaoundé, Cameroon.
authors.forename: Turibio Kuiate
authors.initials: TK
authors.lastname: Tabopda
authors.affiliation: Laboratoire de Pharmacognosie, EA 4267/UFC, UFR des Sciences de Santé, Université de Bourgogne Franche-Comté, 7, Bd. Jeanne d'Arc, BP 87900, 21079 Dijon Cedex, France.
authors.forename: Anne-Claire
authors.initials: AC
authors.lastname: Mitaine-Offer
authors.affiliation: Universität Siegen, FB8, OC-II (AK Ihmels), Adolf-Reichwein-Strasse 2, D-57068 Siegen, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Paululat
authors.affiliation: Cohiro, UFR des Sciences de Santé, 7, Bd. Jeanne d'Arc, BP 87900, 21079 Dijon Cedex, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Delemasure
authors.affiliation: Cohiro, UFR des Sciences de Santé, 7, Bd. Jeanne d'Arc, BP 87900, 21079 Dijon Cedex, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Dutartre
authors.affiliation: Département de Chimie Organique, Université de Yaoundé 1, BP 812 Yaoundé, Cameroon.
authors.forename: Bonaventure Tchaleu
authors.initials: BT
authors.lastname: Ngadjui
authors.affiliation: Laboratoire de Pharmacognosie, EA 4267/UFC, UFR des Sciences de Santé, Université de Bourgogne Franche-Comté, 7, Bd. Jeanne d'Arc, BP 87900, 21079 Dijon Cedex, France. Electronic address: m-a.lacaille-dubois@u-bourgogne.fr.
authors.forename: Marie-Aleth
authors.initials: MA
authors.lastname: Lacaille-Dubois
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002163:Cameroon; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D019668:Liliaceae / Q000737:chemistry*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010840:Phytosterols / Q000737:chemistry; D051381:Rats; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013150:Spirostans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013237:Stereoisomerism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010840:Phytosterols; D012503:Saponins; D013150:Spirostans
keywords: Asparagaceae; Chlorophytum deistelianum; Cytotoxicity; Steroidal saponins