title: Aureolysin of Staphylococcus warneri M accelerates its proteolytic cascade, and participates in biofilm formation.
abstract: The aureolysin (Aur) gene of S. warneri M (aurWM) was cloned and sequenced. Analyses of the aurWM-inactivated mutant (S. warneri Mau) suggested that AurWM was probably associated with efficient processing of the PROM protease (homolog of V8/SspA serine protease), whereas considerable amount of mature-PROC protease (homolog of SspB cysteine protease) accumulated without AurWM. Additionally, AurWM appeared to affect biofilm formation in an uncertain suppressive way.
authors.affiliation: a Food Biochemistry Division , Toyama prefectural Food Research Institute , Toyama , Japan.
authors.forename: Ken-Ji
authors.initials: KJ
authors.lastname: Yokoi
authors.affiliation: b Molecular Biology Group, Faculty of Engineering , Toyama University , Toyama , Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Kuzuwa
authors.affiliation: c Quality Control Group , White Food Industry Co. Ltd. , Nanto , Japan.
authors.forename: Shu-Ichi
authors.initials: S
authors.lastname: Iwasaki
authors.affiliation: d Biochemistry Division, Department of Material Science , Wakayama National College of Technology , Wakayama , Japan.
authors.forename: Ayanori
authors.initials: A
authors.lastname: Yamakawa
authors.affiliation: e Department of Applied Physics and Chemistry , Fukui University of Technology , Fukui , Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Taketo
authors.affiliation: b Molecular Biology Group, Faculty of Engineering , Toyama University , Toyama , Japan.
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Kodaira
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D018441:Biofilms / Q000254:growth & development; D003001:Cloning, Molecular; D057056:Cysteine Proteases / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D008666:Metalloendopeptidases / Q000235:genetics* / Q000378:metabolism; D059748:Proteolysis; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D057057:Serine Proteases / Q000235:genetics* / Q000378:metabolism; D013210:Staphylococcus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D057056:Cysteine Proteases; D057057:Serine Proteases; D008666:Metalloendopeptidases; C115504:aureolysin
keywords: SspA; Staphylococcus warneri; aureolysin; biofilm; metalloprotease