title: Estimation of HIV-1 DNA Level Interfering with Reliability of HIV-1 RNA Quantification Performed on Dried Blood Spots Collected from Successfully Treated Patients.
abstract: The impact of HIV-1 DNA coamplification during HIV-1 RNA quantification on dried blood spots (DBS) was explored. False-positive HIV RNA detection (22/62, 35%) was associated with high HIV-1 DNA levels. Specificity of HIV-1 RNA assays on DBS should be evaluated following manufacturer protocols on samples with HIV-1 DNA levels of ≥1,000 copies/10(6) peripheral blood mononuclear cells.
authors.affiliation: Laboratoire de Virologie, Département des Sciences Biomédicales, Centre MURAZ, Bobo-Dioulasso, Burkina Faso.|INSERM Unité 1058, Université de Montpellier et CHRU de Montpellier, Montpellier, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Zida
authors.affiliation: INSERM Unité 1058, Université de Montpellier et CHRU de Montpellier, Montpellier, France e-tuaillon@chu-montpellier.fr.
authors.forename: Edouard
authors.initials: E
authors.lastname: Tuaillon
authors.affiliation: Département de Pédiatrie, CHU Sanou Souro, Bobo-Dioulasso, Burkina Faso.
authors.forename: Makoura
authors.initials: M
authors.lastname: Barro
authors.affiliation: Laboratoire de Virologie, Département des Sciences Biomédicales, Centre MURAZ, Bobo-Dioulasso, Burkina Faso.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Kwimatouo Lekpa Franchard
authors.affiliation: Laboratoire de Virologie, Département des Sciences Biomédicales, Centre MURAZ, Bobo-Dioulasso, Burkina Faso.
authors.forename: Thérèse
authors.initials: T
authors.lastname: Kagoné
authors.affiliation: Département de Pédiatrie, CHU Sanou Souro, Bobo-Dioulasso, Burkina Faso.
authors.forename: Boubacar
authors.initials: B
authors.lastname: Nacro
authors.affiliation: Laboratoire de Bactériologie-Virologie, CHU Yalgado Ouédraogo, Ouagadougou, Burkina Faso.
authors.forename: Abdoul Salam
authors.initials: AS
authors.lastname: Ouedraogo
authors.affiliation: INSERM Unité 1058, Université de Montpellier et CHRU de Montpellier, Montpellier, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Bolloré
authors.affiliation: INSERM Unité 1058, Université de Montpellier et CHRU de Montpellier, Montpellier, France.
authors.forename: Armen
authors.initials: A
authors.lastname: Sanosyan
authors.affiliation: Laboratoire de Virologie, CHRU Charles Nicole, Rouen, France.
authors.forename: Jean-Christophe
authors.identifier: http://orcid.org/0000-0001-6417-4083
authors.initials: JC
authors.lastname: Plantier
authors.affiliation: Laboratoire de Virologie, Département des Sciences Biomédicales, Centre MURAZ, Bobo-Dioulasso, Burkina Faso.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Meda
authors.affiliation: Laboratoire de Bactériologie-Virologie, CHU Yalgado Ouédraogo, Ouagadougou, Burkina Faso.
authors.forename: Lassana
authors.initials: L
authors.lastname: Sangaré
authors.affiliation: Equipe d'accueil 7327, Université Paris-Descartes, Paris, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Rouzioux
authors.affiliation: Unité VIH/Hépatites, Institut Pasteur du Cambodge, Phnom Penh, Cambodia.
authors.forename: François
authors.initials: F
authors.lastname: Rouet
authors.affiliation: Laboratoire de Virologie, Département des Sciences Biomédicales, Centre MURAZ, Bobo-Dioulasso, Burkina Faso.
authors.forename: Dramane
authors.initials: D
authors.lastname: Kania
mesh_terms: D004279:DNA, Viral / Q000097:blood*; D003890:Desiccation; D003951:Diagnostic Errors; D016903:Drug Monitoring / Q000379:methods; D005189:False Positive Reactions; D015658:HIV Infections / Q000188:drug therapy / Q000821:virology*; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D012367:RNA, Viral / Q000097:blood*; D013048:Specimen Handling / Q000379:methods*; D019562:Viral Load / Q000379:methods*
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral