title: Enhanced expression of endothelial nitric oxide synthase in the myocardium ameliorates the progression of left ventricular hypertrophy in L-arginine treated Wistar-Kyoto rats.
abstract: The present study investigated the role of endothelial nitric oxide synthase (eNOS) enzyme in the development of left ventricular hypertrophy (LVH) in Wistar-Kyoto rats. The effect of L-arginine administration on cardiac structure, arterial stiffness, renal and systemic hemodynamic parameters was studied and the change in expression of eNOS and cystathione γ lyase (CSE) in the myocardium of LVH rats was evaluated. LVH was induced using isoprenaline (5 mg/kg, S.C.) and caffeine (62 mg/L in drinking water) for 14 days. Following to that, L-arginine (1.25 g/L in drinking water) was given for 5 weeks as a donor of NO. eNOS and CSE gene expressions were down regulated in the LVH group by about 35% and 67% respectively when compared to control. However, in the LVH group treated with L-arginine there was up regulation of eNOS by almost 27% and down regulation in CSE by 24% when compared to control (all P < 0.05). Heart index and H2S plasma levels were reduced by almost 53% in the L-arginine treated LVH group compared to the control (all P < 0.05). Mean arterial pressure, heart rate and pulse wave velocity were reduced while renal blood perfusion increased in L-arginine treated LVH rats compared to their untreated counterparts (all P < 0.05). The enhanced expression of eNOS in L-arginine treated LVH rats resulted in the amelioration of oxidative and haemodynamic parameters suggesting that NO system is an important therapeutic target in cardiac and LV hypertrophies.
authors.affiliation: School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang, Malaysia. raza_chohan487@hotmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Ahmad
authors.affiliation: School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang, Malaysia. munavvar@usm.my.
authors.forename: M A
authors.initials: MA
authors.lastname: Sattar
authors.affiliation: School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang, Malaysia.
authors.forename: H A
authors.initials: HA
authors.lastname: Rathore
authors.affiliation: Department of Physiology, University College Cork, Cork, Ireland.
authors.forename: M H
authors.initials: MH
authors.lastname: Abdulla
authors.affiliation: School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang, Malaysia.
authors.forename: S A
authors.initials: SA
authors.lastname: Khan
authors.affiliation: Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: N A
authors.initials: NA
authors.lastname: Abdullah
authors.affiliation: Department of Physiology, University College Cork, Cork, Ireland.
authors.forename: E J
authors.initials: EJ
authors.lastname: Johns
mesh_terms: D000818:Animals; D001120:Arginine / Q000494:pharmacology*; D062186:Arterial Pressure / Q000187:drug effects; D001783:Blood Flow Velocity / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects*; D006321:Heart / Q000187:drug effects*; D006339:Heart Rate / Q000187:drug effects; D006439:Hemodynamics / Q000187:drug effects; D017379:Hypertrophy, Left Ventricular / Q000188:drug therapy* / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D063177:Pulse Wave Analysis / Q000379:methods; D051381:Rats; D011921:Rats, Inbred WKY; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D009569:Nitric Oxide; D001120:Arginine; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat