title: Resveratrol lowers blood pressure in spontaneously hypertensive rats via calcium-dependent endothelial NO production.
abstract: OBJECTIVE
  Resveratrol, a polyphenol of natural compounds, has beneficial cardiovascular effects, many of which are mediated by nitric oxide (NO). Resveratrol increases intracellular calcium and activates AMP-activated protein kinase (AMPK), all of which could increase NO production. We hypothesized that resveratrol via a calcium-dependent NO production lowers blood pressure (BP) in spontaneously hypertensive rats (SHR).
  
  
  METHODS
  Acetylcholine (Ach)-induced endothelium-dependent relaxations in rat aortas were examined by organ chamber. Blood pressures were determined by radiotelemetry methods.
  
  
  RESULTS
  Incubation of isolated aortas from SHR with resveratrol dramatically improved vasorelaxation induced by Ach. Preincubation of aortas with endothelial NO synthase (eNOS) inhibitor or calcium chelant blunted the effects of resveratrol on Ach-induced relaxation, as wells as NO production and eNOS phosphorylation. In animal studies, administration of resveratrol significantly lowered systemic BP in SHR.
  
  
  CONCLUSIONS
  Resveratrol increases endothelial NO production to improve endothelial dysfunction and lowers BP in hypertensive rats, which depends on calcium-eNOS activation.
authors.affiliation: a Department of Cardiology , The First Affiliated Hospital of Medical College, Xi'an Jiao Tong University , Xi'an , China.|b Department of Cardiology , The Fourth Affiliated Hospital of Harbin Medical University , Harbin , China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: b Department of Cardiology , The Fourth Affiliated Hospital of Harbin Medical University , Harbin , China.
authors.forename: Yingnan
authors.initials: Y
authors.lastname: Dai
authors.affiliation: b Department of Cardiology , The Fourth Affiliated Hospital of Harbin Medical University , Harbin , China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Yan
authors.affiliation: b Department of Cardiology , The Fourth Affiliated Hospital of Harbin Medical University , Harbin , China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Shi
authors.affiliation: b Department of Cardiology , The Fourth Affiliated Hospital of Harbin Medical University , Harbin , China.
authors.forename: Jingxiu
authors.initials: J
authors.lastname: Li
authors.affiliation: b Department of Cardiology , The Fourth Affiliated Hospital of Harbin Medical University , Harbin , China.
authors.forename: Jinglu
authors.initials: J
authors.lastname: Liu
authors.affiliation: b Department of Cardiology , The Fourth Affiliated Hospital of Harbin Medical University , Harbin , China.
authors.forename: Li
authors.initials: L
authors.lastname: Cha
authors.affiliation: a Department of Cardiology , The First Affiliated Hospital of Medical College, Xi'an Jiao Tong University , Xi'an , China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Mu
mesh_terms: D000109:Acetylcholine; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology; D001011:Aorta / Q000187:drug effects / Q000503:physiopathology; D001794:Blood Pressure / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D004195:Disease Models, Animal; D006973:Hypertension / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism / Q000494:pharmacology; D014664:Vasodilation / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D013267:Stilbenes; D009569:Nitric Oxide; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III; D000109:Acetylcholine; D000077185:Resveratrol; D002118:Calcium
keywords: Calcium; eNOS; endothelial dysfunction; hypertensive; resveratrol