title: Presence of Chlamydiales DNA in samples negative by broad-range bacterial 16S rRNA PCRs: new insights into chlamydial pathogenic role.
abstract: Since routine eubacterial 16S rRNA PCR does not amplify members of the Chlamydiales order, we tested all samples received in our laboratory during a 10 months period using a pan-Chlamydiales real-time PCR. 3 of 107 samples (2.8%) revealed to be positive, suggesting a role of some Chlamydiales in the pathogenesis of chronic bronchial stenosis or bronchial stenosis superinfection and as agents of orthopaedic prosthesis infections.
authors.affiliation: Department of Laboratory, Institute of Microbiology, Switzerland.
authors.forename: F
authors.initials: F
authors.lastname: Tagini
authors.affiliation: Department of Laboratory, Institute of Microbiology, Switzerland.
authors.forename: K
authors.initials: K
authors.lastname: Jaton
authors.affiliation: Department of Internal Medicine, Service of Infectious Diseases, University Hospital Center, Lausanne, Switzerland.
authors.forename: O
authors.initials: O
authors.lastname: Manuel
authors.affiliation: Department of Laboratory, Institute of Microbiology, Switzerland; Department of Internal Medicine, Service of Infectious Diseases, University Hospital Center, Lausanne, Switzerland.
authors.forename: G
authors.initials: G
authors.lastname: Greub
keywords: Broad-range bacterial 16S rRNA PCR; Chlamydia-related bacteria; Chlamydiales; molecular diagnosis; pan-Chlamydiales PCR