title: CRISPR/Cas9: an advanced tool for editing plant genomes.
abstract: To meet current challenges in agriculture, genome editing using sequence-specific nucleases (SSNs) is a powerful tool for basic and applied plant biology research. Here, we describe the principle and application of available genome editing tools, including zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeat associated CRISPR/Cas9 system. Among these SSNs, CRISPR/Cas9 is the most recently characterized and rapidly developing genome editing technology, and has been successfully utilized in a wide variety of organisms. This review specifically illustrates the power of CRISPR/Cas9 as a tool for plant genome engineering, and describes the strengths and weaknesses of the CRISPR/Cas9 technology compared to two well-established genome editing tools, ZFNs and TALENs.
authors.affiliation: Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, Kharagpur, 721302, India.
authors.forename: Milan Kumar
authors.initials: MK
authors.lastname: Samanta
authors.affiliation: Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, Kharagpur, 721302, India.
authors.forename: Avishek
authors.initials: A
authors.lastname: Dey
authors.affiliation: Department of Human Genetics, University of Michigan, Ann Arbor, MI, 48109, USA. sgayen@umich.edu.
authors.forename: Srimonta
authors.initials: S
authors.lastname: Gayen
mesh_terms: D064113:CRISPR-Cas Systems / Q000235:genetics*; D000072669:Gene Editing / Q000379:methods*; D018390:Gene Targeting; D018745:Genome, Plant / Q000235:genetics*; D010944:Plants / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics*; D000075804:Zinc Finger Nucleases / Q000235:genetics
chemical_list: D000075804:Zinc Finger Nucleases
keywords: CRISPR/Cas9; Genome editing; Plants; Sequence-specific nucleases; TALENs; ZFNs