title: Alterations in the expression of Per1 and Per2 induced by Aβ31-35 in the suprachiasmatic nucleus, hippocampus, and heart of C57BL/6 mouse.
abstract: Patients with Alzheimer's disease (AD) have circadian rhythm disorders, which are mimicked in 3xTg-AD and 5xFAD mouse models. The deposition of β-amyloid protein (Aβ) is an important pathological characteristic of AD, however, its role in inducing alterations in biological rhythms and in the expression of circadian clock-related genes remains elusive. The Per1 and Per2 play complex regulatory roles in biological clocks and are diffusely expressed in the suprachiasmatic nucleus (SCN), hippocampus and heart. In the present study, wheel-running behavioral experiments showed that Aβ31-35, which was administered into the hippocampus, resulted in the disruption of the circadian rhythm of C57BL/6 mice. Furthermore, real-time PCR and western blot analysis showed that Aβ31-35 altered the expression of the Per1 and Per2 in the SCN, hippocampus and heart. These findings provide experimental evidence for circadian rhythm disturbances in patients with AD.
authors.affiliation: Department of Pathology, Shanxi Medical University, Taiyuan 030001, China. Electronic address: 163.wangxh@163.com.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pathology, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Pathology, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Department of Pathology, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Cardiology, Shanxi Dayi Hospital, Taiyuan 030032, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Xueling
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Physiology, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Cao
authors.affiliation: Department of Physiology, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Pathology, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Lingmin
authors.initials: L
authors.lastname: Li
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism* / Q000503:physiopathology; D016229:Amyloid beta-Peptides / Q000008:administration & dosage*; D000818:Animals; D001683:Biological Clocks; D002940:Circadian Rhythm; D006624:Hippocampus / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009043:Motor Activity; D009206:Myocardium / Q000378:metabolism*; D010446:Peptide Fragments / Q000008:administration & dosage; D056950:Period Circadian Proteins / Q000378:metabolism*; D013493:Suprachiasmatic Nucleus / Q000378:metabolism*
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C539023:Per1 protein, mouse; C513312:Per2 protein, mouse; D056950:Period Circadian Proteins
keywords: Aβ31-35; Heart; Hippocampus; Per1; Per2; SCN