title: Cardioprotective Effect of Modified Peroxiredoxins in Retrograde Perfusion of Isolated Rat Heart under Conditions of Oxidative Stress.
abstract: Antioxidant properties of recombinant peroxiredoxin-6 and chimeric protein PSH combining peroxidase and superoxide dismutase activities were studied on the model of retrograde perfusion of isolated rat heart under conditions of H2O2-induced oxidative stress. The exogenous antioxidant proteins exhibited cardioprotective properties manifested in heart rate normalization, maintenance of contractile activity of the myocardium, and prevention of H2O2-induced LPO in oxidative stress. Localization of peroxiredoxin-6 and PSH in the cardiac tissue was determined and myocardial structures most effectively protected by the antioxidant enzymes from ischemia/reperfusion-induced damages were identified. The results suggest that modified peroxiredoxins are promising components of perfusion media for preservation of isolated organs.
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia. e.v.karaduleva@gmail.com.
authors.forename: E V
authors.initials: EV
authors.lastname: Karaduleva
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia.
authors.forename: E K
authors.initials: EK
authors.lastname: Mubarakshina
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia.
authors.forename: M G
authors.initials: MG
authors.lastname: Sharapov
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia.
authors.forename: A E
authors.initials: AE
authors.lastname: Volkova
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia.|Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
authors.forename: O Yu
authors.initials: OY
authors.lastname: Pimenov
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia.
authors.forename: V K
authors.initials: VK
authors.lastname: Ravin
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia.|Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
authors.forename: Yu M
authors.initials: YM
authors.lastname: Kokoz
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia.
authors.forename: V I
authors.initials: VI
authors.lastname: Novoselov
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D002316:Cardiotonic Agents / Q000627:therapeutic use*; D006321:Heart / Q000503:physiopathology; D006339:Heart Rate / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000009:adverse effects; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000517:prevention & control*; D018384:Oxidative Stress / Q000187:drug effects*; D010477:Perfusion; D009195:Peroxidase / Q000378:metabolism; D054464:Peroxiredoxins / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002316:Cardiotonic Agents; D006861:Hydrogen Peroxide; D054464:Peroxiredoxins; D009195:Peroxidase; D013482:Superoxide Dismutase
keywords: oxidative stress; peroxiredoxins; retrograde perfusion of the heart