title: Facile preparation of multifunctional superparamagnetic PHBV microspheres containing SPIONs for biomedical applications.
abstract: The promising potential of magnetic polymer microspheres in various biomedical applications has been frequently reported. However, the surface hydrophilicity of superparamagnetic iron oxide nanoparticles (SPIONs) usually leads to poor or even failed encapsulation of SPIONs in hydrophobic polymer microspheres using the emulsion method. In this study, the stability of SPIONs in poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) solution was significantly increased after surface modification with lauric acid. As a result, magnetic PHBV microspheres with high encapsulation efficiencies (71.0-87.4%) were prepared using emulsion-solvent extraction/evaporation method. Magnetic resonance imaging (MRI) showed significant contrast for the magnetic PHBV microspheres. The toxicity of these magnetic PHBV microspheres towards human T-lymphoma suspension cells and adherent colon carcinoma HT-29 cells was investigated using flow cytometry, and they were shown to be non-toxic in a broad concentration range. A model drug, tetracycline hydrochloride, was used to demonstrate the drug delivery capability and to investigate the drug release behavior of the magnetic PHBV microspheres. The drug was successfully loaded into the microspheres using lauric acid-coated SPIONs as drug carrier, and was released from the microspheres in a diffusion controlled manner. The developed magnetic PHBV microspheres are promising candidates for biomedical applications such as targeted drug delivery and MRI.
authors.affiliation: Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Cauerstrasse 6, 91058 Erlangen, Germany.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Section for Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung Professorship, University Hospital Erlangen, Glückstrasse 10a, 91054 Erlangen, Germany.
authors.lastname: Jan Zaloga
authors.affiliation: Institute of Polymer Materials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Martensstrasse 7, 91058 Erlangen, Germany.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Henriette Schmidt-Burkhardt Chair of Food Chemistry, Department of Chemistry and Pharmacy, University of Erlangen-Nuremberg, Schuhstrasse19, 91052 Erlangen, Germany.
authors.forename: Yufang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Section for Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung Professorship, University Hospital Erlangen, Glückstrasse 10a, 91054 Erlangen, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Janko
authors.affiliation: Henriette Schmidt-Burkhardt Chair of Food Chemistry, Department of Chemistry and Pharmacy, University of Erlangen-Nuremberg, Schuhstrasse19, 91052 Erlangen, Germany.
authors.forename: Monika
authors.initials: M
authors.lastname: Pischetsrieder
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Section for Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung Professorship, University Hospital Erlangen, Glückstrasse 10a, 91054 Erlangen, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Alexiou
authors.affiliation: Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Cauerstrasse 6, 91058 Erlangen, Germany.
authors.forename: Aldo R
authors.initials: AR
authors.lastname: Boccaccini
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D016503:Drug Delivery Systems / Q000379:methods; D019073:HT29 Cells; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008422:Materials Testing; D008863:Microspheres; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry* / Q000494:pharmacology; D013499:Surface Properties; D013752:Tetracycline / Q000737:chemistry / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D000094122:Polyhydroxybutyrates
chemical_list: D058185:Magnetite Nanoparticles; D011091:Polyesters; D013752:Tetracycline; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates