title: IDENTIFICATION OF COENZYME Q10FROM PORPHYRIDIUM PURPUREUM (RHODOPHYTA) BY MATRIX-ASSISTED LASER DESORPTION IONIZATION CURVED FIELD REFLECTRON MASS SPECTROMETRY1.
abstract: Antioxidant agents from natural sources are currently the focus of scientific interest and are part of several natural product screenings. Coenzymes Q (CoQ, ubiquinones) are integral parts of the electron transport chain of the inner mitochondrial membrane. As antioxidants they protect phospholipids against peroxidation and are also involved in various processes of tissue protection. Their natural occurrence was validated for Saccharomyces cerevisiae as CoQ6, for Escherichia coli as CoQ8, and for humans as CoQ10. After carrying out a preparative reversed-phase (RP)-HPLC separation of extracts isolated from unicellular red alga Porphyridium purpureum (Bory) K. M. Drew et R. Ross, it was possible to identify a 2,3-dimethoxy-5-methyl-6-decaprenyl-1,4-benzoquinone (CoQ10) within these extracts using a matrix-assisted laser desorption ionization (MALDI) curved field reflectron (CFR) mass spectrometer. Detected mass fragments showed a high significance and could be structurally interpreted for both commercialized standard and CoQ10isolated from P. purpureum.
authors.affiliation: Institute of Bioprocess Engineering, Friedrich-Alexander University Erlangen-Nuremberg (FAU), Paul-Gordan-Strasse 3, 91052 Erlangen, Germany.
authors.forename: Barbara C
authors.initials: BC
authors.lastname: Klein
authors.affiliation: Institute of Bioprocess Engineering, Friedrich-Alexander University Erlangen-Nuremberg (FAU), Paul-Gordan-Strasse 3, 91052 Erlangen, Germany.
authors.forename: Sebastian J
authors.initials: SJ
authors.lastname: Bartel
authors.affiliation: Institute of Bioprocess Engineering, Friedrich-Alexander University Erlangen-Nuremberg (FAU), Paul-Gordan-Strasse 3, 91052 Erlangen, Germany.
authors.forename: Kai H
authors.initials: KH
authors.lastname: Darsow
authors.affiliation: Institute of Bioprocess Engineering, Friedrich-Alexander University Erlangen-Nuremberg (FAU), Paul-Gordan-Strasse 3, 91052 Erlangen, Germany.
authors.forename: Ivonne
authors.initials: I
authors.lastname: Naumann
authors.affiliation: Institute of Bioprocess Engineering, Friedrich-Alexander University Erlangen-Nuremberg (FAU), Paul-Gordan-Strasse 3, 91052 Erlangen, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Walter
authors.affiliation: Institute of Bioprocess Engineering, Friedrich-Alexander University Erlangen-Nuremberg (FAU), Paul-Gordan-Strasse 3, 91052 Erlangen, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Buchholz
authors.affiliation: Institute of Bioprocess Engineering, Friedrich-Alexander University Erlangen-Nuremberg (FAU), Paul-Gordan-Strasse 3, 91052 Erlangen, Germany.
authors.forename: Harald A
authors.initials: HA
authors.lastname: Lange
keywords: MALDI curved field reflectron mass spectrometer; Porphyridium purpureum; antioxidant; coenzyme Q10