title: Pararhizobium polonicum sp. nov. isolated from tumors on stone fruit rootstocks.
abstract: Five Gram-negative, rod-shaped, non-spore-forming bacteria were isolated from galls on different stone fruit rootstocks in Poland: strains F5.1(T) and F5.3 from Prunus avium F12/1, strains CP3.5 and CP17.2.1 from Prunus avium and strain AL5.1.8 from Prunus cerasifera. On the basis of 16S rDNA phylogeny, the strains cluster together and belong to the genus Pararhizobium with type strain of Pararhizobium herbae (99.6-99.8%) as their closest relative. Phylogenetic analysis of the novel strains using housekeeping genes atpD, recA and rpoB revealed their distinct position separate from other known Rhizobium species and confirmed their relation to P. herbae. DNA-DNA hybridization of strains F5.1(T), with the type strain of P. herbae LMG 25718(T) and Pararhizobium giardinii R-4385(T) revealed 28.3% and 27.9% of DNA-DNA relatedness, respectively. Phenotypic and physiological properties differentiate the novel isolates from other closely related species. On the basis of the results obtained, the five isolates are considered to represent a novel species of the genus Pararhizobium, for which the name Pararhizobium polonicum sp. nov. (type strain F5.1(T)=LMG 28610(T)=CFBP 8359(T)) is proposed.
authors.affiliation: Research Institute of Horticulture, ul. Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland. Electronic address: joanna.pulawska@inhort.pl.
authors.forename: Joanna
authors.initials: J
authors.lastname: Puławska
authors.affiliation: University of Belgrade - Faculty of Agriculture, Nemanjina 6, 11080 Belgrade, Serbia.
authors.forename: Nemanja
authors.initials: N
authors.lastname: Kuzmanović
authors.affiliation: Laboratory of Microbiology, Dept. Biochemistry and Microbiology, Fac. Science, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
authors.forename: Anne
authors.initials: A
authors.lastname: Willems
authors.affiliation: Environmental Genomics and Systems Biology Research Group, Institute of Natural Resource Sciences, Zurich University of Applied Sciences, Einsiedlerstrasse 31, CH-8820 Wädenswil, Switzerland.
authors.forename: Joël F
authors.initials: JF
authors.lastname: Pothier
mesh_terms: D015373:Bacterial Typing Techniques; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D010941:Plant Tumors / Q000382:microbiology; D011044:Poland; D000068220:Prunus avium / Q000382:microbiology*; D000068242:Prunus domestica / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011938:Rec A Recombinases / Q000235:genetics; D012230:Rhizobiaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014157:Transcription Factors / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014157:Transcription Factors; D011938:Rec A Recombinases; D012321:DNA-Directed RNA Polymerases
keywords: Crown gall; DNA–DNA hybridization; Genome; MALDI-TOF MS; MLSA