title: Improvement of the Xpert Carba-R Kit for the Detection of Carbapenemase-Producing Enterobacteriaceae.
abstract: The Xpert Carba-R kit, version 2 (v2), which has been improved for the efficient detection of blaOXA-181 and blaOXA-232 genes, was tested on a collection of 150 well-characterized enterobacterial isolates that had a reduced susceptibility to carbapenems. The performance of the Xpert Carba-R v2 was high, as it was able to detect the five major carbapenemases (NDM, VIM, IMP, KPC, and OXA-48). Thus, it is now well adapted to the carbapenemase-producing Enterobacteriaceae epidemiology of many countries worldwide.
authors.affiliation: Bacteriology-Hygiene Unit, Assistance Publique/Hôpitaux de Paris, Bicêtre Hospital, Le Kremlin-Bicêtre, France EA7361 "Structure, Dynamic, Function and Expression of Broad Spectrum β-Lactamases," Paris-Sud University, LabEx Lermit, Faculty of Medicine, Le Kremlin-Bicêtre, France Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, Le Kremlin-Bicêtre, France Joint Research Unit EERA "Evolution and Ecology of Resistance to Antibiotics," Institut Pasteur-APHP-Université Paris-Sud, Paris, France.
authors.forename: Laurent
authors.identifier: 0000-0001-6596-7384
authors.initials: L
authors.lastname: Dortet
authors.affiliation: Bacteriology-Hygiene Unit, Assistance Publique/Hôpitaux de Paris, Bicêtre Hospital, Le Kremlin-Bicêtre, France Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, Le Kremlin-Bicêtre, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Fusaro
authors.affiliation: Bacteriology-Hygiene Unit, Assistance Publique/Hôpitaux de Paris, Bicêtre Hospital, Le Kremlin-Bicêtre, France EA7361 "Structure, Dynamic, Function and Expression of Broad Spectrum β-Lactamases," Paris-Sud University, LabEx Lermit, Faculty of Medicine, Le Kremlin-Bicêtre, France Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, Le Kremlin-Bicêtre, France Joint Research Unit EERA "Evolution and Ecology of Resistance to Antibiotics," Institut Pasteur-APHP-Université Paris-Sud, Paris, France thierry.naas@bct.aphp.fr.
authors.forename: Thierry
authors.initials: T
authors.lastname: Naas
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D015780:Carbapenems / Q000494:pharmacology; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D015870:Gene Expression; D006801:Humans; D010957:Plasmids / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D011933:Reagent Kits, Diagnostic; D001618:beta-Lactamases / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D011933:Reagent Kits, Diagnostic; D001618:beta-Lactamases; C063912:carbapenemase