title: ALTERATIONS IN BRAIN CREATINE CONCENTRATIONS UNDER LONG-TERM SOCIAL ISOLATION (EXPERIMENTAL STUDY).
abstract: Stress represents one of the main problems of modern humanity. This study was done for understanding more clearly alterations in creatine content of the brain under psycho-emotional stress induced by long-term social isolation. It was shown that under 30 days social isolation creatine amount in the brain was arisen, while decreasing concentrations of synthesizing enzymes (AGAT, GAMT) and creatine transporter protein (CrT). Another important point was that such changes were accompanied by down-regulation of creatine kinase (CK), therefore the enzyme's concentration was lowered. In addition, it was observed that content of phosphocreatine (PCr) and ATP were also reduced, thus indicating down-regulation of energy metabolism of brain that is really a crucial point for its normal functioning. To sum up the results it can be underlined that long-term social isolation has negative influence on energy metabolism of brain; and as a result reduce ATP content, while increase of free creatine concentration, supposedly maintaining maximal balance for ATP amount, but here must be also noted that up-regulated oxidative pathways might have impact on blood brain barrier, resulting on its permeability.
authors.affiliation: I. Javakhishvili Tbilisi State University, Faculty of Exact and Natural Sciences, Department of Biology, Georgia.
authors.forename: N
authors.initials: N
authors.lastname: Koshoridze
authors.affiliation: I. Javakhishvili Tbilisi State University, Faculty of Exact and Natural Sciences, Department of Biology, Georgia.
authors.forename: Z
authors.initials: Z
authors.lastname: Kuchukashvili
authors.affiliation: I. Javakhishvili Tbilisi State University, Faculty of Exact and Natural Sciences, Department of Biology, Georgia.
authors.forename: K
authors.initials: K
authors.lastname: Menabde
authors.affiliation: I. Javakhishvili Tbilisi State University, Faculty of Exact and Natural Sciences, Department of Biology, Georgia.
authors.forename: Sh
authors.initials: Sh
authors.lastname: Lekiashvili
authors.affiliation: I. Javakhishvili Tbilisi State University, Faculty of Exact and Natural Sciences, Department of Biology, Georgia.
authors.forename: M
authors.initials: M
authors.lastname: Koshoridze
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000579:Amidinotransferases / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism*; D003401:Creatine / Q000378:metabolism*; D003402:Creatine Kinase / Q000378:metabolism; D004734:Energy Metabolism; D050937:Guanidinoacetate N-Methyltransferase / Q000378:metabolism; D008297:Male; D026901:Membrane Transport Proteins / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism; D051381:Rats; D012934:Social Isolation
chemical_list: D026901:Membrane Transport Proteins; C080679:creatine transporter; D010725:Phosphocreatine; D000255:Adenosine Triphosphate; C494508:Gamt protein, rat; D050937:Guanidinoacetate N-Methyltransferase; D000579:Amidinotransferases; C020681:glycine amidinotransferase; D003402:Creatine Kinase; D003401:Creatine