title: Isolation of Enterobacter aerogenes carrying blaTEM-1 and blaKPC-3 genes recovered from a hospital Intensive Care Unit.
abstract: Enterobacter aerogenes has recently emerged as an important hospital pathogen. In this study, we showed the emergence of E. aerogenes isolates carrying the blaKPC gene in patients colonized by carbapenem-resistant Klebsiella pneumoniae strains. Two multiresistant E. aerogenes isolates were recovered from bronchial aspirates of two patients hospitalized in the Intensive Care Unit at the "Santa Maria della Scaletta" Hospital, Imola. The antimicrobial susceptibility test showed the high resistance to carbapenems and double-disk synergy test confirmed the phenotype of KPC and AmpC production. Other investigation revealed that ESBL and blaKPC genes were carried on the conjugative pKpQIL plasmid. This is a relevant report in Italy that describes a nosocomial infection due to the production of KPC beta-lactamases by an E. aerogenes isolate in patients previously colonized by K. pneumoniae carbapenem-resistant. In conclusion, it's necessary a continuous monitoring of multidrug-resistant strains for the detection of any KPC-producing bacteria that could expand the circulation of carbapenem-resistant pathogens.
authors.affiliation: Department of Molecular Medicine and Medical Biotechnology, Federico II University Medical School, Naples, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Pulcrano
authors.affiliation: Department of Diagnostic Services, S. Maria della Scaletta Hospital, Imola, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Pignanelli
authors.affiliation: Department of Molecular Medicine and Medical Biotechnology, Federico II University Medical School, Naples, Italy.
authors.forename: Adriana
authors.initials: A
authors.lastname: Vollaro
authors.affiliation: Department of Molecular Medicine and Medical Biotechnology, Federico II University Medical School, Naples, Italy.
authors.forename: Matilde
authors.initials: M
authors.lastname: Esposito
authors.affiliation: Department of Molecular Medicine and Medical Biotechnology, Federico II University Medical School, Naples, Italy.
authors.forename: Vita Dora
authors.initials: VD
authors.lastname: Iula
authors.affiliation: Department of Molecular Medicine and Medical Biotechnology, Federico II University Medical School, Naples, Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Roscetto
authors.affiliation: Department of Molecular Medicine and Medical Biotechnology, Federico II University Medical School, Naples, Italy.
authors.forename: Amata Amy
authors.initials: AA
authors.lastname: Soriano
authors.affiliation: Department of Molecular Medicine and Medical Biotechnology, Federico II University Medical School, Naples, Italy.
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Catania
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D001980:Bronchi / Q000382:microbiology; D003428:Cross Infection / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D021902:Enterobacter aerogenes / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D006761:Hospitals; D006801:Humans; D007362:Intensive Care Units; D007558:Italy; D007711:Klebsiella pneumoniae / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000032:analysis; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D001618:beta-Lactamases; C495220:beta-lactamase KPC-3, Klebsiella pneumoniae; C034413:beta-lactamase TEM-1
keywords: KPC gene; carbapenem resistence; nosocomial infection; surveillance