title: Molecular basis for cytokine biomarkers of complex 3D microtissue physiology in vitro.
abstract: 'Physiologically more-relevant' claims are readily made for cells cultured on any surface or in a scaffold that provides loosely defined 3D geometry. A set of tools to measure culture '3D-ness' more accurately are needed. Such tools should find applications in fields ranging from high-throughput identification of substrates for tissue engineering and regenerative medicine to cell-based screening of drug candidates. Until now, these fields have not provided a consensus for the most promising place to initiate the search. Here, we review recent advances in transcriptomic, proteomic, inflammation and oncology-related pathways, as well as functional studies that strongly point to cytokines as the most likely compounds to form the missing consensus.
authors.affiliation: Cellular Bioengineering Laboratory, College of Engineering, Driftmier Engineering Center, University of Georgia, Athens, GA 30602, USA.
authors.forename: Amish
authors.initials: A
authors.lastname: Asthana
authors.affiliation: Cellular Bioengineering Laboratory, College of Engineering, Driftmier Engineering Center, University of Georgia, Athens, GA 30602, USA. Electronic address: williamk@engr.uga.edu.
authors.forename: William S
authors.initials: WS
authors.lastname: Kisaalita
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques; D016207:Cytokines / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D040901:Proteomics; D023822:Tissue Engineering
chemical_list: D015415:Biomarkers; D016207:Cytokines