title: Microwave, ultrasound, thermal treatments, and bead milling as intensification techniques for extraction of lipids from oleaginous Yarrowia lipolytica yeast for a biojetfuel application.
abstract: In the present work, two different ways of lipids extraction from Yarrowia lipolytica yeast were investigated in order to maximize the extraction yield. Firstly, various modern techniques of extraction including ultrasound, microwave, and bead milling were tested to intensify the efficiency of lipid recovery. Secondly, several pretreatments such as freezing/defrosting, cold drying, bead milling, and microwave prior two washing of mixture solvent of chloroform:methanol (1:2, v/v) were study to evaluate the impact on lipid recovery. All these treatments were compared to conventional maceration, in terms of lipids extraction yield and lipid composition analysis. The main result of this study is the large difference of lipid recovery among treatments and the alteration of lipids profile after microwave and ultrasound techniques.
authors.affiliation: Université d'Avignon et des Pays du Vaucluse, INRA, UMR 408, GREEN Extraction Team, F-84000 Avignon, France.
authors.forename: Alice
authors.initials: A
authors.lastname: Meullemiestre
authors.affiliation: Université d'Avignon et des Pays du Vaucluse, INRA, UMR 408, GREEN Extraction Team, F-84000 Avignon, France.
authors.forename: Cassandra
authors.initials: C
authors.lastname: Breil
authors.affiliation: Université d'Avignon et des Pays du Vaucluse, INRA, UMR 408, GREEN Extraction Team, F-84000 Avignon, France. Electronic address: maryline.vian@univ-avignon.fr.
authors.forename: Maryline
authors.initials: M
authors.lastname: Abert-Vian
authors.affiliation: Université d'Avignon et des Pays du Vaucluse, INRA, UMR 408, GREEN Extraction Team, F-84000 Avignon, France.
authors.forename: Farid
authors.initials: F
authors.lastname: Chemat
mesh_terms: D056804:Biofuels; D008055:Lipids / Q000302:isolation & purification*; D008872:Microwaves; D014465:Ultrasonics; D025062:Yarrowia / Q000737:chemistry*
chemical_list: D056804:Biofuels; D008055:Lipids
keywords: Bead milling; Lipid extraction; Microwave; Oleaginous microorganisms; Ultrasound