title: High-throughput profiling of microbial community structures in an ANAMMOX-UASB reactor treating high-strength wastewater.
abstract: In this study, the microbial community structure was assessed in an anaerobic ammonium oxidation-upflow anaerobic sludge blanket (ANAMMOX-UASB) reactor treating high-strength wastewater (approximately 700 mg N L(-1) in total nitrogen) by employing Illumina high-throughput sequencing analysis. The reactor was started up and reached a steady state in 26 days by seeding mature ANAMMOX granules, and a high nitrogen removal rate (NRR) of 2.96 kg N m(-3) day(-1) was obtained at 13.2∼17.6 °C. Results revealed that the abundance of ANAMMOX bacteria increased during the operation, though it occupied a low proportion in the system. The phylum Planctomycetes was only 8.39 % on day 148 and Candidatus Brocadia was identified as the dominant ANAMMOX species with a percentage of 2.70 %. The phylum of Chloroflexi, Bacteroidetes, and Proteobacteria constituted a percentage up to 70 % in the community, of which the Chloroflexi and Bacteroidetes were likely to be related to the sludge granulation. In addition, it was found that heterotrophic denitrifying bacteria of Denitratisoma belonging to Proteobacteria phylum occupied a large proportion (22.1∼23.58 %), which was likely caused by the bacteria lysis and decay with the internal carbon source production. The SEM images also showed that plenty of other microorganisms existed in the ANAMMOX-UASB reactor.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090, China.
authors.forename: Shenbin
authors.initials: S
authors.lastname: Cao
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovory Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing, 100124, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Du
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovory Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing, 100124, China.|Department of Civil and Environmental Engineering, University of Connecticut, Storrs, CT, 06269, USA.
authors.forename: Baikun
authors.initials: B
authors.lastname: Li
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090, China.
authors.forename: Nanqi
authors.initials: N
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090, China. pyz@bjut.edu.cn.|Key Laboratory of Beijing for Water Quality Science and Water Environment Recovory Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing, 100124, China. pyz@bjut.edu.cn.
authors.forename: Yongzhen
authors.initials: Y
authors.lastname: Peng
mesh_terms: D041963:Bacteroidetes / Q000254:growth & development; D001673:Biodegradation, Environmental; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D041862:Chloroflexi / Q000254:growth & development; D004269:DNA, Bacterial / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing; D009584:Nitrogen / Q000378:metabolism; D020560:Proteobacteria / Q000254:growth & development; D017422:Sequence Analysis, DNA; D062065:Wastewater / Q000382:microbiology*; D018508:Water Purification
chemical_list: D004269:DNA, Bacterial; D009584:Nitrogen; D062065:Wastewater
keywords: ANAMMOX; Denitrifying bacterium; High-strength wastewater; High-throughput sequencing analysis; Microbial community structure