title: Distinct temporal changes in host cell lncRNA expression during the course of an adenovirus infection.
abstract: The deregulation of cellular long non-coding RNA (lncRNA) expression during a human adenovirus infection was studied by deep sequencing. Expression of lncRNAs increased substantially following the progression of the infection. Among 645 significantly expressed lncRNAs, the expression of 398 was changed more than 2-fold. More than 80% of them were up-regulated and 80% of them were detected during the late phase. Based on the genomic locations of the deregulated lncRNAs in relation to known mRNAs and miRNAs, they were predicted to be involved in growth, structure, apoptosis and wound healing in the early phase, cell proliferation in the intermediate phase and protein synthesis, modification and transport in the late phase. The most significant functions of cellular RNA-binding proteins, previously shown to interact with the deregulated lncRNAs identified here, are involved in RNA splicing, nuclear export and translation events. We hypothesize that adenoviruses exploit the lncRNA network to optimize their reproduction.
authors.affiliation: The Beijer Laboratory, Dept. of Immunology, Genetics and Pathology, Uppsala University, S-751 85 Uppsala, Sweden. Electronic address: Hongxing.Zhao@igp.uu.se.
authors.forename: Hongxing
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria 3086, Australia.
authors.forename: Maoshan
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Chemistry-BMC, Analytical Chemistry, Science for Life Laboratory, Uppsala University, Box 599, SE-751 24 Uppsala, Sweden.
authors.forename: Sara Bergström
authors.initials: SB
authors.lastname: Lind
authors.affiliation: The Beijer Laboratory, Dept. of Immunology, Genetics and Pathology, Uppsala University, S-751 85 Uppsala, Sweden.
authors.forename: Ulf
authors.initials: U
authors.lastname: Pettersson
mesh_terms: D000256:Adenoviridae / Q000502:physiology*; D017209:Apoptosis; D049109:Cell Proliferation; D019295:Computational Biology; D005347:Fibroblasts / Q000378:metabolism* / Q000821:virology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D054884:Host-Pathogen Interactions; D006801:Humans; D058977:Molecular Sequence Annotation; D005810:Multigene Family; D061251:Primary Cell Culture; D011544:Pseudogenes; D016372:RNA, Antisense / Q000235:genetics; D062085:RNA, Long Noncoding / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D016372:RNA, Antisense; D062085:RNA, Long Noncoding
keywords: Adenovirus infection; Antisense RNAs; Cellular gene expression; Long non-coding RNA (lncRNA); RNA sequencing; RNA-binding proteins (RBPs); lncRNA expression profile