title: Diminished miR-613 expression as a novel prognostic biomarker for human ovarian cancer.
abstract: OBJECTIVE
  miR-613 may suppress ovarian cancer development and progression. We wished to assess the potential prognostic value of miR-613 expression analysis in ovarian cancer.
  
  
  METHODS
  A total of 236 pairs of human ovarian cancer and matched normal tissue specimens were collected. Resected tissue specimens were obtained during the operation and were snap-frozen in liquid nitrogen for subsequent RNA extraction. Total RNA was extracted from tissue specimens, and miR-613 expression was quantified using TaqMan qPCR. The miR-613 expression was normalized to expression of U6 as an internal standard.
  
  
  RESULTS
  miR-613 expression in ovarian cancer tissue specimens was significantly (p < 0.001) lower than that in matched normal adjacent tissue specimens. Furthermore, within ovarian cancer tissue specimens, 114 specimens exhibited lower levels of miR-613 ("low expression" group), whereas 122 specimens showed higher levels of this miR ("high expression" group). Low expression of miR-613 showed significant associations with the FIGO stage (p < 0.001), tumour grade (p < 0.001), and lymph node metastases (p < 0.001). Furthermore, low miR-613 expression was associated with lower progression-free and overall survival (respectively, p < 0.01 and < 0.0001). Finally, the multivariate analysis using the Cox proportional hazards model showed that miR-613 expression levels, FIGO stage, and lymph node metastases were independently associated with progression-free and overall survival in patients with ovarian cancer.
  
  
  CONCLUSIONS
  miR-613 expression levels are low in ovarian cancer tissue and correlate with progression-free and overall survival. Thus, miR-613 may be useful as a prognostic marker in ovarian cancer.
authors.affiliation: Department of Obstetrics and Gynecology, First People's Hospital of Shangqiu, Shangqiu City, Henan, China. Zhangxxpl@163.com.
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: H
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D002648:Child; D018450:Disease Progression; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; C561374:MIRN613 microRNA, human; D035683:MicroRNAs