title: TLR Agonist Augments Prophylactic Potential of Acid Inducible Antigen Rv3203 against Mycobacterium tuberculosis H37Rv in Experimental Animals.
abstract: In general, the members of Lip gene family of Mycobacterium tuberculosis evoke strong immune response in the host. Keeping this fact into consideration, we investigated role of Rv3203, a cell wall associated protein with lipolytic activity, in imparting protection against experimental murine tuberculosis. The data of the present study suggested that archaeosome encapsulated Rv3203 induce strong lymphocyte proliferation, up-regulated Th-1 biased cytokines profile, increased expression of co-stimulatory markers on both antigen presenting cells and T lymphocytes. The immuno-prophylactic response was further modulated by exposure of the animals to zymosan, a TLR2/6 agonist, prior to immunization with archaeosome encapsulated Rv3203. Interestingly, pre-treatment of experimental animals with zymosan boosted strong immunological memory as compared to archaeosome encapsulated Rv3203 as well as BCG vaccine. We conclude that priming of immunized animal with TLR agonist followed by immunization with archaeosomes encapsulated Rv3203 offer substantial protection against tuberculosis infection and could be a potential subunit vaccine based prophylactic strategy.
authors.affiliation: Molecular Immunology Laboratory, Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India.
authors.forename: Owais
authors.initials: O
authors.lastname: Mohammad
authors.affiliation: Department of Biotechnology, Panjab University, Chandigarh, India.
authors.forename: Jagdeep
authors.initials: J
authors.lastname: Kaur
authors.affiliation: Department of Biotechnology, Panjab University, Chandigarh, India.
authors.forename: Gurpreet
authors.initials: G
authors.lastname: Singh
authors.affiliation: Molecular Immunology Laboratory, Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India.
authors.forename: Syed Mohd
authors.initials: SM
authors.lastname: Faisal
authors.affiliation: Molecular Immunology Laboratory, Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India.
authors.forename: Asim
authors.initials: A
authors.lastname: Azhar
authors.affiliation: Molecular Immunology Laboratory, Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India.
authors.forename: Mohd Ahmar
authors.initials: MA
authors.lastname: Rauf
authors.affiliation: National JALMA Institute for Leprosy & other Mycobacterial Diseases, Tajganj, Agra, India.
authors.forename: Umesh Dutt
authors.initials: UD
authors.lastname: Gupta
authors.affiliation: National JALMA Institute for Leprosy & other Mycobacterial Diseases, Tajganj, Agra, India.
authors.forename: Pushpa
authors.initials: P
authors.lastname: Gupta
authors.affiliation: National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Pal
authors.affiliation: Women's College, Aligarh Muslim University, Aligarh, India.
authors.forename: Swaleha
authors.initials: S
authors.lastname: Zubair
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology* / Q000302:isolation & purification; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007114:Immunization; D007156:Immunologic Memory; D008807:Mice, Inbred BALB C; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D017510:Protein Folding; D018417:Th1 Cells / Q000378:metabolism; D051193:Toll-Like Receptors / Q000819:agonists*; D014376:Tuberculosis / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000942:Antigens, Bacterial; D016207:Cytokines; D051193:Toll-Like Receptors