title: Calcitonin and vitamin D3 have high therapeutic potential for improving diabetic mandibular growth.
abstract: The goal of this study was to assess the effect of the intermittent combination of an antiresorptive agent (calcitonin) and an anabolic agent (vitamin D3) on treating the detrimental effects of Type 1 diabetes mellitus (DM) on mandibular bone formation and growth. Forty 3-week-old male Wistar rats were divided into four groups: the control group (normal rats), the control C+D group (normal rats injected with calcitonin and vitamin D3), the diabetic C+D group (diabetic rats injected with calcitonin and vitamin D3) and the diabetic group (uncontrolled diabetic rats). An experimental DM condition was induced in the male Wistar rats in the diabetic and diabetic C+D groups using a single dose of 60 mg·kg(-1) body weight of streptozotocin. Calcitonin and vitamin D3 were simultaneously injected in the rats of the control C+D and diabetic C+D groups. All rats were killed after 4 weeks, and the right mandibles were evaluated by micro-computed tomography and histomorphometric analysis. Diabetic rats showed a significant deterioration in bone quality and bone formation (diabetic group). By contrast, with the injection of calcitonin and vitamin D3, both bone parameters and bone formation significantly improved (diabetic C+D group) (P < 0.05). These findings suggest that these two hormones might potentially improve various bone properties.
authors.affiliation: Orthodontic Department, Faculty of Dentistry, King Abdulaziz University, Jeddah, Saudi Arabia.|Department of Orofacial Development and Function, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.|Alexandria University, Alexandria, Egypt.
authors.forename: Mona A
authors.initials: MA
authors.lastname: Abbassy
authors.affiliation: Department of Orofacial Development and Function, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Ippei
authors.initials: I
authors.lastname: Watari
authors.affiliation: Conservative Dentistry Department, Faculty of Dentistry, King Abdulaziz University, Jeddah, Saudi Arabia.|Cariology and Operative Dentistry Department, Graduate School of Medical and Dental Sciences, Tokyo Medical & Dental University, Tokyo, Japan.|Conservative Dentistry Department, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
authors.forename: Ahmed S
authors.initials: AS
authors.lastname: Bakry
authors.affiliation: Department of Orofacial Development and Function, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ono
authors.affiliation: Orthodontic Department, Faculty of Dentistry, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Ali H
authors.initials: AH
authors.lastname: Hassan
mesh_terms: D000818:Animals; D002116:Calcitonin / Q000494:pharmacology*; D002762:Cholecalciferol / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D003922:Diabetes Mellitus, Type 1 / Q000503:physiopathology*; D008297:Male; D008334:Mandible / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000254:growth & development*; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D055114:X-Ray Microtomography
chemical_list: D002762:Cholecalciferol; D002116:Calcitonin