title: Effects of label-dose permethrin administration in yearling beef cattle: I. Bull reproductive function and testicular histopathology.
abstract: Pyrethroid administration to a wide variety of laboratory animals has been shown to cause detrimental effects on male fertility, including sperm quality, by means of endocrine disruption. The objective of this experiment was to study the effects of a commercial, permethrin-containing pour-on product on reproductive variables and testicular histopathology of yearling beef bulls. Black Angus bulls (n = 60; aged 369 ± 17 days; 511 ± 33 kg; 6.2 ± 0.5 body condition scores) were assigned to either (1) saline control (CON) or (2) permethrin pour-on administered at label dose (PYR). Blood samples were collected, and industry standard breeding soundness examinations (BSE), via electroejaculation, were performed on all bulls at 5 days before and 14 days after treatment. Progressive sperm motility and eosin-nigrosin-stained sperm were analyzed using high-power phase-contrast microscopy. Plasma testosterone concentrations were analyzed via radioimmunoassay. Bulls were slaughtered at 34 days, and one testicle per bull was randomly collected for histologic examination. Change in sperm motility between BSEs was not different because of treatment; sperm morphology however improved across treatments, but PYR bulls had less improvement in percent of head (P < 0.001) sperm abnormalities compared to CON, resulting in less improvement of primary abnormalities (P = 0.04). Nonetheless, morphological differences did not change the overall outcome for satisfactory breeder status. Change in testosterone concentration did not differ because of treatment. Histopathologic examination identified that testicular degeneration and tubule diameter did not differ as a result of treatment. It should be noted, however, that degeneration score (higher score having more degeneration) was positively correlated with primary abnormalities (P < 0.01; r = 0.35) and negatively correlated with normal sperm cells (P < 0.001; r = -0.43). In summary, these data indicate that a single use of permethrin at label dose in yearling Angus bulls results in minimal detrimental effects on sperm morphology but not to a degree that impacts the ability of bulls to pass a standard BSE.
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA.
authors.forename: Tyler M
authors.initials: TM
authors.lastname: Dohlman
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA.
authors.forename: Patrick E
authors.initials: PE
authors.lastname: Phillips
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA.
authors.forename: Darin M
authors.initials: DM
authors.lastname: Madson
authors.affiliation: Armstrong Research and Demonstration Farm, Iowa State University, Lewis, Iowa, USA.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Clark
authors.affiliation: Department of Animal Science, Iowa State University, Ames, Iowa, USA. Electronic address: pgunn@iastate.edu.
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Gunn
mesh_terms: D000818:Animals; D002417:Cattle; D052244:Endocrine Disruptors / Q000008:administration & dosage / Q000009:adverse effects*; D005298:Fertility / Q000187:drug effects; D008297:Male; D026023:Permethrin / Q000008:administration & dosage / Q000009:adverse effects*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects; D013737:Testis / Q000187:drug effects / Q000473:pathology
chemical_list: D052244:Endocrine Disruptors; D026023:Permethrin
keywords: Bull; Endocrine disruption; Permethrin; Pyrethroid; Sperm; Testicular degeneration