title: Modular synthesis of N-glycans and arrays for the hetero-ligand binding analysis of HIV antibodies.
abstract: A new class of broadly neutralizing antibodies (bNAbs) from HIV donors has been reported to target the glycans on gp120--a glycoprotein found on the surface of the virus envelope--thus renewing hope of developing carbohydrate-based HIV vaccines. However, the version of gp120 used in previous studies was not from human T cells and so the glycosylation pattern could be somewhat different to that found in the native system. Moreover, some antibodies recognized two different glycans simultaneously and this cannot be detected with the commonly used glycan microarrays on glass slides. Here, we have developed a glycan microarray on an aluminium-oxide-coated glass slide containing a diverse set of glycans, including homo- and mixed N-glycans (high-mannose, hybrid and complex types) that were prepared by modular chemo-enzymatic methods to detect the presence of hetero-glycan binding behaviours. This new approach allows rapid screening and identification of optimal glycans recognized by neutralizing antibodies, and could speed up the development of HIV-1 vaccines targeting cell surface glycans.
authors.affiliation: Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 115, Taiwan.|Chemical Biology and Molecular Biophysics, Taiwan International Graduate Program, Academia Sinica, Taiwan.|Institute of Biochemical Sciences, National Taiwan University, 1 Roosevelt Road, Section 4, Taipei, 106, Taiwan.|CHO Pharma Inc., Park Street, Nangang District, Taipei 11503, Taiwan.
authors.forename: Sachin S
authors.initials: SS
authors.lastname: Shivatare
authors.affiliation: Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 115, Taiwan.|Institute of Biochemical Sciences, National Taiwan University, 1 Roosevelt Road, Section 4, Taipei, 106, Taiwan.
authors.forename: Shih-Huang
authors.initials: SH
authors.lastname: Chang
authors.affiliation: The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
authors.forename: Tsung-I
authors.identifier: http://orcid.org/0000-0002-8355-6017
authors.initials: TI
authors.lastname: Tsai
authors.affiliation: Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 115, Taiwan.
authors.forename: Susan Yu
authors.identifier: http://orcid.org/0000-0002-5710-6960
authors.initials: SY
authors.lastname: Tseng
authors.affiliation: Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 115, Taiwan.
authors.forename: Vidya S
authors.initials: VS
authors.lastname: Shivatare
authors.affiliation: CHO Pharma Inc., Park Street, Nangang District, Taipei 11503, Taiwan.
authors.forename: Yih-Shyan
authors.initials: YS
authors.lastname: Lin
authors.affiliation: Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 115, Taiwan.
authors.forename: Yang-Yu
authors.initials: YY
authors.lastname: Cheng
authors.affiliation: Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 115, Taiwan.
authors.forename: Chien-Tai
authors.initials: CT
authors.lastname: Ren
authors.affiliation: Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 115, Taiwan.
authors.forename: Chang-Chun David
authors.initials: CC
authors.lastname: Lee
authors.affiliation: Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 115, Taiwan.|Chemical Biology and Molecular Biophysics, Taiwan International Graduate Program, Academia Sinica, Taiwan.
authors.forename: Sujeet
authors.initials: S
authors.lastname: Pawar
authors.affiliation: CHO Pharma Inc., Park Street, Nangang District, Taipei 11503, Taiwan.
authors.forename: Charng-Sheng
authors.initials: CS
authors.lastname: Tsai
authors.affiliation: CHO Pharma Inc., Park Street, Nangang District, Taipei 11503, Taiwan.
authors.forename: Hao-Wei
authors.initials: HW
authors.lastname: Shih
authors.affiliation: CHO Pharma Inc., Park Street, Nangang District, Taipei 11503, Taiwan.
authors.forename: Yi-Fang
authors.initials: YF
authors.lastname: Zeng
authors.affiliation: Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 115, Taiwan.|The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
authors.forename: Chi-Hui
authors.initials: CH
authors.lastname: Liang
authors.affiliation: Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: Peter D
authors.initials: PD
authors.lastname: Kwong
authors.affiliation: The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
authors.forename: Dennis R
authors.initials: DR
authors.lastname: Burton
authors.affiliation: Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 115, Taiwan.
authors.forename: Chung-Yi
authors.initials: CY
authors.lastname: Wu
authors.affiliation: Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 115, Taiwan.|The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
authors.forename: Chi-Huey
authors.initials: CH
authors.lastname: Wong
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology; D057134:Antibodies, Neutralizing / Q000276:immunology*; D015483:HIV Antibodies / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D008024:Ligands; D011134:Polysaccharides / Q000138:chemical synthesis* / Q000737:chemistry / Q000276:immunology
chemical_list: D016915:AIDS Vaccines; D057134:Antibodies, Neutralizing; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; D008024:Ligands; D011134:Polysaccharides