title: The use of native fluorescence analysis of synovial fluid in the diagnosis of medial compartment disease in medium- and large-breed dogs.
abstract: We assumed that proteins are most likely responsible for synovial fluid fluorescence and that changes detected in fluorescence intensity are most likely the result of changes in the concentration of fluorescent proteins. Synchronous fluorescent matrices from synovial fluid samples were measured in the excitation wavelength range of 200-350 nm using a luminescence spectrophotometer. The synchronous matrix of synovial fluid consists of 2 dominant fluorescent centers (F1 and F2) in the ultraviolet region. The fluorescence intensities of both centers were significantly higher in pathological samples, with p = 0.001 (a 59% increase of the median value) for the F1 center and p = 0.002 (a 52% increase of the median value) for the F2 center. Receiver operating characteristic analysis confirmed that synovial fluid autofluorescence is a significant predictor of medial compartment disease in dogs, with the area under the curve at 0.776 (F1) and 0.778 (F2). We did not detect any differences in the autofluorescence of synovial fluid between male and female, or any breed-based changes. No position changes of fluorescent centers were recorded in the synovial fluid in diseased dogs compared with healthy dogs. The synovial fluid metabolic fingerprint of canine patients with medial compartment disease differed from that of healthy dogs. Our study demonstrated the feasibility of synovial fluid fingerprinting to identify disease-specific profiles of synovial fluid metabolites.
authors.affiliation: Small Animal Clinic, Department of Surgery, Orthopaedics, Radiology and Reproduction, (Bilská, Ledecký, Hluchý), University of Veterinary Medicine and Pharmacy, Košice, SlovakiaDepartment of Chemistry, Biochemistry and Biophysics (Šteffeková), University of Veterinary Medicine and Pharmacy, Košice, SlovakiaDepartment of Medical and Clinical Biochemistry and LABMED, a.s., Faculty of Medicine, Pavol Jozef Šafárik University, Košice, Slovakia (Birková, Mareková)Department of Animal Physiology, Institute of Biology and Ecology, Pavol Jozef Šafárik University, Košice, Slovakia (Kisková) kami851983@gmail.com.
authors.forename: Kamila
authors.initials: K
authors.lastname: Bilská
authors.affiliation: Small Animal Clinic, Department of Surgery, Orthopaedics, Radiology and Reproduction, (Bilská, Ledecký, Hluchý), University of Veterinary Medicine and Pharmacy, Košice, SlovakiaDepartment of Chemistry, Biochemistry and Biophysics (Šteffeková), University of Veterinary Medicine and Pharmacy, Košice, SlovakiaDepartment of Medical and Clinical Biochemistry and LABMED, a.s., Faculty of Medicine, Pavol Jozef Šafárik University, Košice, Slovakia (Birková, Mareková)Department of Animal Physiology, Institute of Biology and Ecology, Pavol Jozef Šafárik University, Košice, Slovakia (Kisková).
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Šteffeková
authors.affiliation: Small Animal Clinic, Department of Surgery, Orthopaedics, Radiology and Reproduction, (Bilská, Ledecký, Hluchý), University of Veterinary Medicine and Pharmacy, Košice, SlovakiaDepartment of Chemistry, Biochemistry and Biophysics (Šteffeková), University of Veterinary Medicine and Pharmacy, Košice, SlovakiaDepartment of Medical and Clinical Biochemistry and LABMED, a.s., Faculty of Medicine, Pavol Jozef Šafárik University, Košice, Slovakia (Birková, Mareková)Department of Animal Physiology, Institute of Biology and Ecology, Pavol Jozef Šafárik University, Košice, Slovakia (Kisková).
authors.forename: Anna
authors.initials: A
authors.lastname: Birková
authors.affiliation: Small Animal Clinic, Department of Surgery, Orthopaedics, Radiology and Reproduction, (Bilská, Ledecký, Hluchý), University of Veterinary Medicine and Pharmacy, Košice, SlovakiaDepartment of Chemistry, Biochemistry and Biophysics (Šteffeková), University of Veterinary Medicine and Pharmacy, Košice, SlovakiaDepartment of Medical and Clinical Biochemistry and LABMED, a.s., Faculty of Medicine, Pavol Jozef Šafárik University, Košice, Slovakia (Birková, Mareková)Department of Animal Physiology, Institute of Biology and Ecology, Pavol Jozef Šafárik University, Košice, Slovakia (Kisková).
authors.forename: Mária
authors.initials: M
authors.lastname: Mareková
authors.affiliation: Small Animal Clinic, Department of Surgery, Orthopaedics, Radiology and Reproduction, (Bilská, Ledecký, Hluchý), University of Veterinary Medicine and Pharmacy, Košice, SlovakiaDepartment of Chemistry, Biochemistry and Biophysics (Šteffeková), University of Veterinary Medicine and Pharmacy, Košice, SlovakiaDepartment of Medical and Clinical Biochemistry and LABMED, a.s., Faculty of Medicine, Pavol Jozef Šafárik University, Košice, Slovakia (Birková, Mareková)Department of Animal Physiology, Institute of Biology and Ecology, Pavol Jozef Šafárik University, Košice, Slovakia (Kisková).
authors.forename: Valent
authors.initials: V
authors.lastname: Ledecký
authors.affiliation: Small Animal Clinic, Department of Surgery, Orthopaedics, Radiology and Reproduction, (Bilská, Ledecký, Hluchý), University of Veterinary Medicine and Pharmacy, Košice, SlovakiaDepartment of Chemistry, Biochemistry and Biophysics (Šteffeková), University of Veterinary Medicine and Pharmacy, Košice, SlovakiaDepartment of Medical and Clinical Biochemistry and LABMED, a.s., Faculty of Medicine, Pavol Jozef Šafárik University, Košice, Slovakia (Birková, Mareková)Department of Animal Physiology, Institute of Biology and Ecology, Pavol Jozef Šafárik University, Košice, Slovakia (Kisková).
authors.forename: Marián
authors.initials: M
authors.lastname: Hluchý
authors.affiliation: Small Animal Clinic, Department of Surgery, Orthopaedics, Radiology and Reproduction, (Bilská, Ledecký, Hluchý), University of Veterinary Medicine and Pharmacy, Košice, SlovakiaDepartment of Chemistry, Biochemistry and Biophysics (Šteffeková), University of Veterinary Medicine and Pharmacy, Košice, SlovakiaDepartment of Medical and Clinical Biochemistry and LABMED, a.s., Faculty of Medicine, Pavol Jozef Šafárik University, Košice, Slovakia (Birková, Mareková)Department of Animal Physiology, Institute of Biology and Ecology, Pavol Jozef Šafárik University, Košice, Slovakia (Kisková).
authors.forename: Terézia
authors.initials: T
authors.lastname: Kisková
mesh_terms: D000818:Animals; D016022:Case-Control Studies; D004283:Dog Diseases / Q000175:diagnosis* / Q000473:pathology; D004285:Dogs; D004551:Elbow Joint; D005260:Female; D008297:Male; D010003:Osteoarthritis / Q000175:diagnosis / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000662:veterinary; D013582:Synovial Fluid / Q000737:chemistry*
keywords: Dogs; fluorescent spectroscopy; medial compartment disease; synovial fluid