title: Proteomic Investigation of Rhizoctonia solani AG 4 Identifies Secretome and Mycelial Proteins with Roles in Plant Cell Wall Degradation and Virulence.
abstract: Rhizoctonia solani AG 4 is a soilborne necrotrophic fungal plant pathogen that causes economically important diseases on agronomic crops worldwide. This study used a proteomics approach to characterize both intracellular proteins and the secretome of R. solani AG 4 isolate Rs23A under several growth conditions, the secretome being highly important in pathogenesis. From over 500 total secretome and soluble intracellular protein spots from 2-D gels, 457 protein spots were analyzed and 318 proteins positively matched with fungal proteins of known function by comparison with available R. solani genome databases specific for anastomosis groups 1-IA, 1-IB, and 3. These proteins were categorized to possible cellular locations and functional groups and for some proteins their putative roles in plant cell wall degradation and virulence. The majority of the secreted proteins were grouped to extracellular regions and contain hydrolase activity.
authors.affiliation: Agricultural Research Service, U.S. Department of Agriculture , Beltsville, Maryland 20705, United States.
authors.forename: Dilip K
authors.initials: DK
authors.lastname: Lakshman
authors.affiliation: Agricultural Research Service, U.S. Department of Agriculture , Beltsville, Maryland 20705, United States.
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Roberts
authors.affiliation: Agricultural Research Service, U.S. Department of Agriculture , Beltsville, Maryland 20705, United States.
authors.forename: Wesley M
authors.initials: WM
authors.lastname: Garrett
authors.affiliation: Agricultural Research Service, U.S. Department of Agriculture , Beltsville, Maryland 20705, United States.
authors.forename: Savithiry S
authors.initials: SS
authors.lastname: Natarajan
authors.affiliation: Computer and Information Sciences, Towson University , Towson, Maryland 21252, United States.
authors.forename: Omar
authors.initials: O
authors.lastname: Darwish
authors.affiliation: Computer and Information Sciences, Towson University , Towson, Maryland 21252, United States.
authors.forename: Nadim
authors.initials: N
authors.lastname: Alkharouf
authors.affiliation: Pathogen Genomics, KAUST , Thuwal, Saudi Arabia 23955.
authors.forename: Arnab
authors.initials: A
authors.lastname: Pain
authors.affiliation: Agricultural Research Service, U.S. Department of Agriculture , Beltsville, Maryland 20705, United States.
authors.forename: Farooq
authors.initials: F
authors.lastname: Khan
authors.affiliation: Department of Plant Pathology, MPUA&T , Udaipur, PIN 327001, India.
authors.forename: Prashant P
authors.initials: PP
authors.lastname: Jambhulkar
authors.affiliation: Department of Plant Pathology, University of Nebraska , Lincoln, Nebraska 68583, United States.
authors.forename: Amitava
authors.initials: A
authors.lastname: Mitra
mesh_terms: D005656:Fungal Proteins / Q000378:metabolism; D059828:Plant Cells / Q000378:metabolism*; D010944:Plants / Q000235:genetics*; D040901:Proteomics; D012232:Rhizoctonia / Q000737:chemistry*; D014774:Virulence / Q000502:physiology*
chemical_list: D005656:Fungal Proteins
keywords: 2-D proteomics; Rhizoctonia solani AG 4; secretome; virulence