title: Nonsteroidal anti-inflammatory drugs enhance IgE-mediated activation of human basophils in patients with food anaphylaxis dependent on and independent of nonsteroidal anti-inflammatory drugs.
abstract: Nonsteroidal anti-inflammatory drugs (NSAIDs) act as cofactors worsening the allergic reactions induced by food allergens.
  
  
  
  The aim of this study was to evaluate the effect of both lysine acetylsalicylate (L-ASA) (non-selective cyclooxygenase (COX) inhibitor) and valdecoxib (selective COX-2 inhibitor) in basophils activated by peach lipid transfer protein (Pru p 3) in patients with food-dependent NSAID-induced anaphylaxis (FDNIA).
  
  
  
  Twenty Pru p 3-allergic patients with FDNIA group, eleven peach anaphylaxis not exacerbated by NSAIDs (no-NSAID group) and 5 healthy volunteers were recruited. Basophil activation (BA) was measured as expression of CD63 (Flow(2) CAST(™) ; Bühlmann(®) ), after stimulation with Pru p 3, both alone and in combination with L-ASA (1.13, 3.38 and 6.78 mm) or valdecoxib (0.87, 7.8 and 31.25 μm).
  
  
  
  Basophils from no-NSAID group were significantly more reactive and sensitive to Pru p 3 than those from the FDNIA group. In both groups, an increase in BA was observed when basophils were exposed to Pru p 3 and L-ASA. In the FDNIA group, valdecoxib partially terminates the BA induced by Pru p 3, whereas in the no-NSAID group, a dual effect was observed depending on the concentration tested.
  
  
  
  This study indicates that subjects with food-induced anaphylaxis differ from FDNIA subjects in the higher reactivity and sensitivity of their basophils to allergen challenge. We have shown a direct effect of NSAIDs on basophils using a human model of FDNIA. Our results also suggest that selective COX2 inhibitors might be a safe alternative. BA test may be a useful tool in the study of the pathogenic mechanism of the cofactor phenomenon.
authors.affiliation: Servei d'Immunologia, Centre de Diagnostic Biomedic (CDB), Hospital Clinic, Universitat de Barcelona, Barcelona, Spain.|Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Pascal
authors.affiliation: Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.|Unitat d'Al·lergia, Servei de Pneumologia, Hospital Clinic, Universitat de Barcelona, Barcelona, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Muñoz-Cano
authors.affiliation: Servei d'Immunologia, Centre de Diagnostic Biomedic (CDB), Hospital Clinic, Universitat de Barcelona, Barcelona, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Milà
authors.affiliation: Clinica Universitaria de Navarra (CUN), Pamplona, Spain.
authors.forename: M L
authors.initials: ML
authors.lastname: Sanz
authors.affiliation: Centro de Biotecnologia y Genomica de Plantas, Universidad Politecnica de Madrid-Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria, Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Diaz-Perales
authors.affiliation: Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.|Unitat d'Al·lergia, Servei de Pneumologia, Hospital Clinic, Universitat de Barcelona, Barcelona, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Sánchez-López
authors.affiliation: Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.|Unitat d'Al·lergia, Servei de Pneumologia, Hospital Clinic, Universitat de Barcelona, Barcelona, Spain.
authors.forename: A
authors.initials: A
authors.lastname: García-Moral
authors.affiliation: Servei d'Immunologia, Centre de Diagnostic Biomedic (CDB), Hospital Clinic, Universitat de Barcelona, Barcelona, Spain.|Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Juan
authors.affiliation: Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.|Unitat d'Al·lergia, Servei de Pneumologia, Hospital Clinic, Universitat de Barcelona, Barcelona, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Valero
authors.affiliation: Servei d'Immunologia, Centre de Diagnostic Biomedic (CDB), Hospital Clinic, Universitat de Barcelona, Barcelona, Spain.|Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Yagüe
authors.affiliation: Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.|Unitat d'Al·lergia, Servei de Pneumologia, Hospital Clinic, Universitat de Barcelona, Barcelona, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Picado
authors.affiliation: Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.|Unitat d'Al·lergia, Servei de Pneumologia, Hospital Clinic, Universitat de Barcelona, Barcelona, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Bartra
mesh_terms: D000328:Adult; D000485:Allergens / Q000276:immunology; D000707:Anaphylaxis / Q000175:diagnosis / Q000276:immunology; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000009:adverse effects*; D001491:Basophils / Q000187:drug effects* / Q000276:immunology*; D015415:Biomarkers; D018450:Disease Progression; D005260:Female; D005502:Food / Q000009:adverse effects; D005512:Food Hypersensitivity / Q000175:diagnosis / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D016130:Immunophenotyping; D008297:Male; D008875:Middle Aged; D012720:Severity of Illness Index; D055815:Young Adult
chemical_list: D000485:Allergens; D000894:Anti-Inflammatory Agents, Non-Steroidal; D015415:Biomarkers; D007073:Immunoglobulin E
keywords: anaphylaxis; basophil activation test; cofactors; food allergy; nonsteroidal anti-inflammatory drugs