title: Application of water-soluble nanofilters for collection of airborne Mycobacterium tuberculosis DNA in hospital wards.
abstract: A simple inexpensive technique for collection of airborne biomarkers of nosocomial infections is described. Biomarkers were collected on water-soluble electrospun nanofilters attached to a household vacuum cleaner from 6-10m(3) of air in 10-15min within several wards of a tuberculosis clinic. Filters were then dissolved in water and tested for the presence of the IS6110 and regX3 genes of Mycobacterium tuberculosis (MTB) using real-time polymerase chain reaction. It was demonstrated that trace amounts of airborne MTB DNA (>3gene copies/m(3)) were always present in the air and on the surfaces in the wards occupied with tuberculosis patients having positive smear tests.
authors.affiliation: Phthisiopulmonology Research Institute of the Sechenov First State Medical University, Moscow, Russian Federation.
authors.forename: M A
authors.initials: MA
authors.lastname: Vladimirsky
authors.affiliation: Phthisiopulmonology Research Institute of the Sechenov First State Medical University, Moscow, Russian Federation.
authors.forename: L K
authors.initials: LK
authors.lastname: Shipina
authors.affiliation: Phthisiopulmonology Research Institute of the Sechenov First State Medical University, Moscow, Russian Federation.
authors.forename: E S
authors.initials: ES
authors.lastname: Makeeva
authors.affiliation: Phthisiopulmonology Research Institute of the Sechenov First State Medical University, Moscow, Russian Federation.
authors.forename: Y S
authors.initials: YS
authors.lastname: Alyapkina
authors.affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 3, Institutskaya Str., Moscow Region, Pushchino, Russian Federation.
authors.forename: A Y
authors.initials: AY
authors.lastname: Mikheev
authors.affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 3, Institutskaya Str., Moscow Region, Pushchino, Russian Federation; The National Center for Biodefense and Infectious Diseases, George Mason University, Manassas, VA 20110, USA. Electronic address: vmorozov@gmu.edu.
authors.forename: V N
authors.initials: VN
authors.lastname: Morozov
mesh_terms: D000391:Air Microbiology; D001431:Bacteriological Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D006761:Hospitals; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D049329:Nanostructures; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012995:Solubility; D013048:Specimen Handling / Q000379:methods*
chemical_list: D004269:DNA, Bacterial
keywords: Aerosol; Mycobacteria detection; Polymerase chain reaction; Water-soluble filters