title: Metagenomic bacterial community profiles of chicken embryo gastrointestinal tract by using T-RFLP analysis.
abstract: Thirty microbial phylotypes of microorganisms were found in the gastrointestinal tract of chicken belonging to the Hajseks White breed, and 38 phylotypes were found in the gastrointestinal tract of chicken belonging to the Hajseks Brown breed. The microbiome of the gastrointestinal tract of the chicken embryos of the Hajseks White breed was dominated by the typical representatives of avian intestinal microflora--bacteria of the family Enterobacteriaceae (47.3%), orders Actinomycetales (13.6%) and Bifidobacteriales (20.6%), and the family Lachnospiraceae (1.1%). The microbiome of the gastrointestinal tract of the chicken embryos of the Hajseks Brown breed was dominated by the pathogenic bacteria of the order Rickettsiales (94.8%). The metagenome of gastrointestinal tract of both breeds also contained a small number of genes of unidentified bacteria.
authors.affiliation: BIOTROPH+ Ltd., St. Petersburg, Russia.
authors.forename: L A
authors.initials: LA
authors.lastname: Ilina
authors.affiliation: BIOTROPH+ Ltd., St. Petersburg, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Yildirim
authors.affiliation: BIOTROPH+ Ltd., St. Petersburg, Russia.
authors.forename: I N
authors.initials: IN
authors.lastname: Nikonov
authors.affiliation: BIOTROPH+ Ltd., St. Petersburg, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Filippova
authors.affiliation: BIOTROPH+ Ltd., St. Petersburg, Russia.
authors.forename: G Y
authors.initials: GY
authors.lastname: Laptev
authors.affiliation: BIOTROPH+ Ltd., St. Petersburg, Russia.
authors.forename: N I
authors.initials: NI
authors.lastname: Novikova
authors.affiliation: All-Russia Research and Technological Institute of Poultry, Sergiev Posad, Moscow oblast, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Grozina
authors.affiliation: All-Russia Research and Technological Institute of Poultry, Sergiev Posad, Moscow oblast, Russia.
authors.forename: T N
authors.initials: TN
authors.lastname: Lenkova
authors.affiliation: All-Russia Research and Technological Institute of Poultry, Sergiev Posad, Moscow oblast, Russia. vard13@yandex.ru.
authors.forename: V A
authors.initials: VA
authors.lastname: Manukyan
authors.affiliation: All-Russia Research and Technological Institute of Poultry, Sergiev Posad, Moscow oblast, Russia.
authors.forename: I A
authors.initials: IA
authors.lastname: Egorov
authors.affiliation: All-Russia Research and Technological Institute of Poultry, Sergiev Posad, Moscow oblast, Russia.
authors.forename: V I
authors.initials: VI
authors.lastname: Fisinin
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D002642:Chick Embryo; D016680:Genome, Bacterial; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000382:microbiology*; D054892:Metagenome; D064307:Microbiota; D016996:Rickettsieae / Q000235:genetics / Q000302:isolation & purification