title: Epigenome confrontation triggers immediate reprogramming of DNA methylation and transposon silencing in Arabidopsis thaliana F1 epihybrids.
abstract: Genes and transposons can exist in variable DNA methylation states, with potentially differential transcription. How these epialleles emerge is poorly understood. Here, we show that crossing an Arabidopsis thaliana plant with a hypomethylated genome and a normally methylated WT individual results, already in the F1 generation, in widespread changes in DNA methylation and transcription patterns. Novel nonparental and heritable epialleles arise at many genic loci, including a locus that itself controls DNA methylation patterns, but with most of the changes affecting pericentromeric transposons. Although a subset of transposons show immediate resilencing, a large number display decreased DNA methylation, which is associated with de novo or enhanced transcriptional activation and can translate into transposon mobilization in the progeny. Our findings reveal that the combination of distinct epigenomes can be viewed as an epigenomic shock, which is characterized by a round of epigenetic variation creating novel patterns of gene and TE regulation.
authors.affiliation: Laboratoire Génétique Reproduction et Développement, CNRS, UMR 6293, INSERM U1103, F-63001 Clermont-Ferrand, France; Laboratoire Génétique Reproduction et Développement, Université Clermont Auvergne, Université Blaise Pascal, BP 10448, F-63000 Clermont-Ferrand, France;
authors.forename: Mélanie
authors.initials: M
authors.lastname: Rigal
authors.affiliation: Department of Molecular Biology, Max Planck Institute for Developmental Biology, D-72076 Tuebingen, Germany.
authors.forename: Claude
authors.initials: C
authors.lastname: Becker
authors.affiliation: Laboratoire Génétique Reproduction et Développement, CNRS, UMR 6293, INSERM U1103, F-63001 Clermont-Ferrand, France; Laboratoire Génétique Reproduction et Développement, Université Clermont Auvergne, Université Blaise Pascal, BP 10448, F-63000 Clermont-Ferrand, France;
authors.forename: Thierry
authors.initials: T
authors.lastname: Pélissier
authors.affiliation: Laboratoire Génétique Reproduction et Développement, CNRS, UMR 6293, INSERM U1103, F-63001 Clermont-Ferrand, France; Laboratoire Génétique Reproduction et Développement, Université Clermont Auvergne, Université Blaise Pascal, BP 10448, F-63000 Clermont-Ferrand, France;
authors.forename: Romain
authors.initials: R
authors.lastname: Pogorelcnik
authors.affiliation: Department of Molecular Biology, Max Planck Institute for Developmental Biology, D-72076 Tuebingen, Germany.
authors.forename: Jane
authors.initials: J
authors.lastname: Devos
authors.affiliation: Laboratoire Génétique Reproduction et Développement, CNRS, UMR 6293, INSERM U1103, F-63001 Clermont-Ferrand, France; Laboratoire Génétique Reproduction et Développement, Université Clermont Auvergne, Université Blaise Pascal, BP 10448, F-63000 Clermont-Ferrand, France;
authors.forename: Yoko
authors.initials: Y
authors.lastname: Ikeda
authors.affiliation: Department of Molecular Biology, Max Planck Institute for Developmental Biology, D-72076 Tuebingen, Germany weigel@tue.mpg.de olivier.mathieu@univ-bpclermont.fr.
authors.forename: Detlef
authors.initials: D
authors.lastname: Weigel
authors.affiliation: Laboratoire Génétique Reproduction et Développement, CNRS, UMR 6293, INSERM U1103, F-63001 Clermont-Ferrand, France; Laboratoire Génétique Reproduction et Développement, Université Clermont Auvergne, Université Blaise Pascal, BP 10448, F-63000 Clermont-Ferrand, France; weigel@tue.mpg.de olivier.mathieu@univ-bpclermont.fr.
authors.forename: Olivier
authors.initials: O
authors.lastname: Mathieu
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D019175:DNA Methylation; D004251:DNA Transposable Elements; D044127:Epigenesis, Genetic; D020868:Gene Silencing; D018745:Genome, Plant; D006824:Hybridization, Genetic; D014158:Transcription, Genetic
chemical_list: D004251:DNA Transposable Elements
keywords: Arabidopsis; DNA methylation; gene silencing; transcription; transposable elements