title: Sepsis-induced impairment of neutrophil chemotaxis on a microfluidic chip.
abstract: This study aimed to design a microfluidic chip to measure neutrophil chemotaxis, which is a convenient assay to assess the severity and prognosis of sepsis, and to study the mechanisms involved in the variation of neutrophil chemotaxis. Neutrophil chemotaxis was investigated in this microfluidic device by measuring the migration speed of neutrophils following the LPS concentration gradient stimulus. Neutrophils of 32 sepsis patients were divided into three groups according to the seriousness of physician-diagnosed sepsis, and 12 healthy individuals served as controls. Statistical significance was set at an alpha value of P<0.05. Neutrophil chemotaxis was significantly decreased following the seriousness of sepsis. By contrast, in septic neutrophils, the expression of TLR2 was significantly increased, whereas the expression of CXCR2 was significantly decreased. Neutrophil chemotaxis in sepsis was significantly reduced as compared to healthy individuals. We speculated that impaired neutrophil chemotaxis in sepsis was probably mediated by the TLR2-CXCR2 pathway.
authors.affiliation: Department of Emergency Medicine, Affiliated Zhongshan Hospital of Dalian University, DaLian, LiaoNing 116001, China. Electronic address: dllxg@126.com.
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Medicine, Dalian University, DaLian, LiaoNing 116622, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Lv
authors.affiliation: School of Pharmaceutical Science and Technology, Dalian University of Technology, DaLian, LiaoNing 116024, China.
authors.forename: Yueyang
authors.initials: Y
authors.lastname: Qu
authors.affiliation: School of Pharmaceutical Science and Technology, Dalian University of Technology, DaLian, LiaoNing 116024, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, DaLian, LiaoNing 116011, China.
authors.forename: Bingcheng
authors.initials: B
authors.lastname: Lin
authors.affiliation: Nanjing University of Chinese Medicine, NanJing, JiangSu 210023, China.
authors.forename: Libin
authors.initials: L
authors.lastname: Zhan
authors.affiliation: Department of Emergency Medicine, Affiliated Zhongshan Hospital of Dalian University, DaLian, LiaoNing 116001, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Emergency Medicine, Affiliated Zhongshan Hospital of Dalian University, DaLian, LiaoNing 116001, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Fan
mesh_terms: D002465:Cell Movement; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte; D006801:Humans; D044085:Microfluidics / Q000379:methods*; D009504:Neutrophils / Q000276:immunology*; D023063:Receptors, Interleukin-8B / Q000378:metabolism*; D018805:Sepsis / Q000175:diagnosis / Q000276:immunology*; D015398:Signal Transduction; D051195:Toll-Like Receptor 2 / Q000378:metabolism*
chemical_list: D023063:Receptors, Interleukin-8B; C495342:TLR2 protein, human; D051195:Toll-Like Receptor 2
keywords: CXCR2; Microfluidic chip; Neutrophil chemotaxis; Sepsis; TLR2; TLR2-CXCR2 pathway