title: Comparison of blood microsampling with DBS and conventional blood collection techniques used in a midazolam biostudy.
abstract: BACKGROUND
  Quantitative DBS LC-MS/MS assay for midazolam was used to compare two sample collection techniques (venipuncture and finger prick) and the midazolam concentrations measured in plasma samples, DBS and dried plasma spots.
  
  
  METHODS
  Midazolam was extracted from DBS cards and compared with whole blood collected from usual venipuncture. Dried plasma spots were also compared with plasma. The blood volume used as well as the temperature impact during the blood and plasma deposits was evaluated. Midazolam was administrated to six healthy subjects during a clinical trial to obtained blood and plasma samples for the statistical comparison.
  
  
  CONCLUSIONS
  The method for midazolam using DBS was validated and showed an excellent performance. Excellent correlations were observed when the same collection procedures were used.
authors.affiliation: inVentiv Health Clinical, 2500 Einstein, Quebec, QC G1P 0A2, Canada.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Lachance
authors.affiliation: inVentiv Health Clinical, 2500 Einstein, Quebec, QC G1P 0A2, Canada.
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Théberge
authors.affiliation: inVentiv Health Clinical, 2500 Einstein, Quebec, QC G1P 0A2, Canada.
authors.forename: Guy
authors.initials: G
authors.lastname: Havard
authors.affiliation: inVentiv Health Clinical, 2500 Einstein, Quebec, QC G1P 0A2, Canada.
authors.forename: Dominique
authors.initials: D
authors.lastname: Anctil
authors.affiliation: inVentiv Health Clinical, 2500 Einstein, Quebec, QC G1P 0A2, Canada.
authors.forename: Olivier
authors.initials: O
authors.lastname: Barrière
authors.affiliation: inVentiv Health Clinical, 2500 Einstein, Quebec, QC G1P 0A2, Canada.
authors.forename: Sara
authors.initials: S
authors.lastname: Croteau
authors.affiliation: inVentiv Health Clinical, 2500 Einstein, Quebec, QC G1P 0A2, Canada.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Pellerin
authors.affiliation: Former Senior Director at inVentiv Health Clinical, 2500 Einstein, Quebec, QC G1P 0A2, Canada.
authors.forename: Ann
authors.initials: A
authors.lastname: Lévesque
mesh_terms: D019540:Area Under Curve; D001800:Blood Specimen Collection; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D059788:Dried Blood Spot Testing; D006207:Half-Life; D006801:Humans; D008874:Midazolam / Q000097:blood* / Q000493:pharmacokinetics / Q000592:standards; D012372:ROC Curve; D012015:Reference Standards; D053719:Tandem Mass Spectrometry / Q000592:standards; D013696:Temperature
chemical_list: D008874:Midazolam
keywords: DBS; DPS; blood volume deposited; finger prick; influence of temperature; method validation; microsampling; midazolam; recovery; venipuncture