title: Accurate Detection of Rifampicin-Resistant Mycobacterium Tuberculosis Strains.
abstract: In 2013 alone, the death rate among the 9.0 million people infected with Mycobacterium tuberculosis (TB) worldwide was around 14%, which is unacceptably high. An empiric treatment of patients infected with TB or drug-resistant Mycobacterium tuberculosis (MDR-TB) strain can also result in the spread of MDR-TB. The diagnostic tools which are rapid, reliable, and have simple experimental protocols can significantly help in decreasing the prevalence rate of MDR-TB strain. We report the evaluation of the 9G technology based 9G DNAChips that allow accurate detection and discrimination of TB and MDR-TB-RIF. One hundred and thirteen known cultured samples were used to evaluate the ability of 9G DNAChip in the detection and discrimination of TB and MDR-TB-RIF strains. Hybridization of immobilized probes with the PCR products of TB and MDR-TB-RIF strains allow their detection and discrimination. The accuracy of 9G DNAChip was determined by comparing its results with sequencing analysis and drug susceptibility testing. Sequencing analysis showed 100% agreement with the results of 9G DNAChip. The 9G DNAChip showed very high sensitivity (95.4%) and specificity (100%).
authors.affiliation: Biometrix Technology, Inc. 202 BioVenture Plaza, Chuncheon 200-161, Korea. hanlimsk@empal.com.
authors.forename: Keum-Soo
authors.initials: KS
authors.lastname: Song
authors.affiliation: Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 200-702, Korea. satish_nimse@hallym.ac.kr.
authors.forename: Satish Balasaheb
authors.initials: SB
authors.lastname: Nimse
authors.affiliation: Korea Mycobacterium Resource Center (KMRC), The Korean Institute of Tuberculosis, Cheongju-si 28158, Korea. hatchingbird@yahoo.co.kr.
authors.forename: Hee Jin
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Korea Mycobacterium Resource Center (KMRC), The Korean Institute of Tuberculosis, Cheongju-si 28158, Korea. nonr@daum.net.
authors.forename: Jeongseong
authors.initials: J
authors.lastname: Yang
authors.affiliation: Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 200-702, Korea. tskim@hallym.ac.kr.
authors.forename: Taisun
authors.initials: T
authors.lastname: Kim
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004352:Drug Resistance, Microbial / Q000235:genetics*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000302:isolation & purification* / Q000472:pathogenicity; D012293:Rifampin / Q000627:therapeutic use; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D012293:Rifampin
keywords: accuracy; diagnosis; drug-resistant tuberculosis; sensitivity; specificity; tuberculosis