title: Functional interaction between nonreceptor tyrosine kinase c-Abl and SR-Rich protein RBM39.
abstract: RBM39, also known as splicing factor HCC1.4, acts as a transcriptional coactivator for the steroid nuclear receptors JUN/AP-1, ESR1/ER-α and ESR2/ER-β. RBM39 is involved in the regulation of the transcriptional responses of these steroid nuclear receptors and promotes transcriptional initiation. In this paper, we report that RBM39 interacts with the nonreceptor tyrosine kinase c-Abl. Both the Src homology (SH) 2 and SH3 domains of c-Abl interact with RBM39. The major tyrosine phosphorylation sites on RBM39 that are phosphorylated by c-Abl are Y95 and Y99, as demonstrated by liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) and mutational analysis. c-Abl was shown boost the transcriptional coactivation activity of RBM39 for ERα and PRβ in a tyrosine kinase-dependent manner. The results suggest that mammalian c-Abl plays an important role in steroid hormone receptor-mediated transcription by regulating RBM39.
authors.affiliation: Beijing Institute of Biotechnology, 27 Taiping Rd, Haidian District, Beijing 100850, China.
authors.forename: Sanyue
authors.initials: S
authors.lastname: Mai
authors.affiliation: General Navy Hospital of PLA, 6 Fucheng Rd, Haidian District, Beijing 100037, China.
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Qu
authors.affiliation: Beijing Institute of Biotechnology, 27 Taiping Rd, Haidian District, Beijing 100850, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: Beijing Institute of Biotechnology, 27 Taiping Rd, Haidian District, Beijing 100850, China.
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Beijing Institute of Biotechnology, 27 Taiping Rd, Haidian District, Beijing 100850, China. Electronic address: liux931932@163.com.
authors.forename: Xuan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Beijing Institute of Biotechnology, 27 Taiping Rd, Haidian District, Beijing 100850, China. Electronic address: cao_c@sohu.com.
authors.forename: Cheng
authors.initials: C
authors.lastname: Cao
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D002460:Cell Line; D002853:Chromatography, Liquid; D004252:DNA Mutational Analysis; D047628:Estrogen Receptor alpha / Q000378:metabolism; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D009687:Nuclear Proteins / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding; D016315:Proto-Oncogene Proteins c-abl / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D015533:Transcriptional Activation; D020798:Two-Hybrid System Techniques; D018909:src Homology Domains
chemical_list: D047628:Estrogen Receptor alpha; C084105:HCC1 autoantigen; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D011980:Receptors, Progesterone; C097314:progesterone receptor B; D016315:Proto-Oncogene Proteins c-abl
keywords: Interact; Phosphorylation; RBM39; Transcriptional coactivation; c-Abl