title: Effect of Genotype and Environment on Salvia miltiorrhiza Roots Using LC/MS-Based Metabolomics.
abstract: Salvia miltiorrhiza (S. miltiorrhiza) Bunge is broadly used as herbal medicine for the clinical treatments of cardiovascular and cerebrovascular diseases. Despite its commercial and medicinal values, few systematic studies on the metabolome of S. miltiorrhiza roots have been carried out so far. We systematically described the metabolic profiles of S. miltiorrhiza using high pressure liquid chromatography mass spectrometry (LC/MS) in conjunction with multivariate statistical analyses, aimed at monitoring their biological variations of secondary metabolites related to three locations and four S. miltiorrhiza genotypes. A total of 40 bioactive constituents were putatively annotated in S. miltiorrhiza root samples. This study found that both the same S. miltiorrhiza genotype growing at three different locations and four S. miltiorrhiza genotypes growing at the same location had significant metabonomic differences identified by the principal component analysis (PCA) approach. By using orthogonal projection to latent structure with discriminant analysis (OPLS-DA), 16 and 14 secondary metabolites can be used as potential location-specific and genotype-specific markers in S. miltiorrhiza, respectively. The specificity of LC/MS profiles offered a powerful tool to discriminate S. miltiorrhiza samples according to genotypes or locations.
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian 271018, China. sdauzhaoq@163.com.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian 271018, China. szqsdau@163.com.
authors.forename: Zhenqiao
authors.initials: Z
authors.lastname: Song
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian 271018, China. xinshf@126.com.
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Fang
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian 271018, China. mingwangxingmomo@163.com.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian 271018, China. guolianlin2013@163.com.
authors.forename: Linlin
authors.initials: L
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian 271018, China. tea1022@163.com.
authors.forename: Tian
authors.initials: T
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian 271018, China. wangjh@sdau.edu.cn.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Wang
mesh_terms: D002853:Chromatography, Liquid; D004777:Environment; D005838:Genotype; D013058:Mass Spectrometry; D055442:Metabolome / Q000235:genetics*; D055432:Metabolomics; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D025341:Principal Component Analysis; D027561:Salvia miltiorrhiza / Q000235:genetics / Q000378:metabolism*
keywords: LC-MS; Salvia miltiorrhiza Bunge; metabolite profiling; multivariate analysis; secondary metabolites