title: Comparative Evaluation of Nimesulide-Loaded Nanoparticles for Anticancer Activity Against Breast Cancer Cells.
abstract: Recent clinical and epidemiological researches have declared that non-steroidal anti-inflammatory agents may display as antineoplastic agents and indicate pro-apoptotic and antiproliferative effects on cancer cells. The major purpose of this research was to develop a novel poly(ethyleneglycol)-block-poly(ε-caprolactone) (PEG-b-PCL) nano-sized particles encapsulated with nimesulide (NMS), a selective COX-2 inhibitor, and to evaluate its anticancer activity against MCF-7 breast cancer cells. NMS-encapsulated PEG-b-PCL nanoparticles were fabricated using three different production techniques: (i) by emulsion-solvent evaporation using a high shear homogenizer, (ii) by emulsion-solvent evaporation using an ultrasonicator, and (iii) by nanoprecipitation. Nanoparticles were evaluated with respect to the entrapment efficiency, size characteristics, drug release rates, thermal behavior, cell viability assays, and apoptosis. The resulting nanoparticles were found to be spherical shapes with negative surface charges. The average diameter of all nanoparticles ranged between 148.5 and 307.2 nm. In vitro release profiles showed that all nanoparticles exhibited a biphasic release pattern. NMS-loaded PEG-b-PCL nanoparticles demonstrated significant anticancer activity against MCF-7 breast cancer cells in a dose-dependent manner, and the effects of nanoparticles on cell proliferation were significantly affected by the preparation techniques. The nanoparticles developed in this work displayed higher potential for the NMS delivery against breast cancer treatment for the future.
authors.affiliation: Faculty of Pharmacy, Department of Pharmaceutical Technology, Ankara University, 06100, Tandogan, Ankara, Turkey.
authors.forename: Ceyda Tuba
authors.initials: CT
authors.lastname: Sengel-Turk
authors.affiliation: Faculty of Pharmacy, Department of Pharmaceutical Technology, Ankara University, 06100, Tandogan, Ankara, Turkey. cogan@pharmacy.ankara.edu.tr.
authors.forename: Canan
authors.initials: C
authors.lastname: Hascicek
authors.affiliation: Faculty of Pharmacy, Department of Biochemistry, Ankara University, 06100, Tandogan, Ankara, Turkey.
authors.forename: Filiz
authors.initials: F
authors.lastname: Bakar
authors.affiliation: Faculty of Pharmacy, Department of Pharmaceutical Technology, Ankara University, 06100, Tandogan, Ankara, Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Simsek
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000138:chemical synthesis / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000188:drug therapy*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D052246:Cyclooxygenase 2 Inhibitors / Q000737:chemistry / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry; D065546:Drug Liberation; D004655:Emulsions / Q000737:chemistry / Q000494:pharmacology; D005260:Female; D006801:Humans; D007783:Lactones / Q000737:chemistry; D061986:MCF-7 Cells; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013449:Sulfonamides / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000970:Antineoplastic Agents; D052246:Cyclooxygenase 2 Inhibitors; D004337:Drug Carriers; D004655:Emulsions; D007783:Lactones; D012997:Solvents; D013449:Sulfonamides; C521904:poly(ethylene glycol)-block-poly(epsilon-caprolactone); D011092:Polyethylene Glycols; C012655:nimesulide
keywords: MCF-7 cells; PEG-block-PCL; apoptosis; nimesulide; polymeric nanoparticles