title: Using primate neural stem cells cultured in self-assembling peptide nanofiber scaffolds to repair injured spinal cords in rats.
abstract: METHODS
  Transplanted primates' neural stem cells (NSCs) tissue engineering complex into spinal cord injury (SCI) model rats, analyze and evaluate the long-term effects of repairing.
  
  
  OBJECTIVE
  Primate NSCs were cultured in self-assembling peptide nanofiber scaffolds to repair SCI.
  
  
  METHODS
  Sun Yat-sen Memorial Hospital, Guangzhou, China.
  
  
  METHODS
  Primate NSCs were isolated and cultured in self-assembling peptide nanofiber scaffolds. T10 SCI model was established; the rats were randomly divided into four groups: NSC plus self-assembling peptide scaffold group; NSC group; self-assembling peptide scaffold group; and control group. Immunohistochemical staining and electronic microscope were used to investigate the growth and differentiation of transplanted NSCs. The motor function of the hind limbs of rats was evaluated (P<0.05 was considered as statistically significant).
  
  
  RESULTS
  NSCs and NSCs cultured in self-assembling peptide nanofiber scaffolds could be induced to differentiation into neurons, glial cells and oligodendrocytes in vitro. The primate NSC culture was established in self-assembling peptide scaffolds. No significant difference was seen in the differentiation rate between primate NSCs cultured in self-assembling peptide nanofiber scaffolds and primate NSCs cultured in regular medium. The motor function of the hind limbs in the NSC plus self-assembling peptide scaffold group was significantly better than that of the other three groups. In addition, the NSCs of the NSC group mainly differentiated into astrocytes.
  
  
  CONCLUSIONS
  Transplantation of primate NSCs cultured in self-assembling peptide scaffolds is efficient for repairing the injured spinal cord and for improving the motor function of spinal cord in rats.
  
  
  BACKGROUND
  The National Natural Science Foundation of China; Science and Technology Office of Guangdong Province.
authors.affiliation: Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou China.
authors.forename: J-C
authors.initials: JC
authors.lastname: Ye
authors.affiliation: Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou China.|Department of Orthopedics, Zhuhai People's Hospital, Zhuhai, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Biotherapy Centre, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Y-F
authors.initials: YF
authors.lastname: Wu
authors.affiliation: Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou China.
authors.forename: P
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou China.
authors.forename: Y
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou China.
authors.forename: L
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou China.
authors.forename: M-J
authors.initials: MJ
authors.lastname: Ma
authors.affiliation: Department of Histology and Embryology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.
authors.forename: Y-S
authors.initials: YS
authors.lastname: Zeng
authors.affiliation: Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou China.
authors.forename: H-Y
authors.initials: HY
authors.lastname: Shen
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004622:Embryo, Mammalian; D005260:Female; D035002:Lower Extremity / Q000503:physiopathology; D008252:Macaca fascicularis; D008854:Microscopy, Electron; D009043:Motor Activity / Q000502:physiology; D057139:Nanofibers / Q000648:ultrastructure; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000502:physiology / Q000648:ultrastructure; D009836:Oligodendroglia / Q000502:physiology / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012720:Severity of Illness Index; D013116:Spinal Cord / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D013119:Spinal Cord Injuries / Q000601:surgery*; D033581:Stem Cell Transplantation / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D009419:Nerve Tissue Proteins