title: Assessment of IgE and IgG4 Binding Capacities of Cow's Milk Proteins Selectively Altered by Proteases.
abstract: Specific IgE and IgG4 have been reported to play key roles in the context of IgE-mediated cow's milk allergy (CMA), but the persistence of their epitopes in milk hydrolysates has not been evaluated. Using sera from 32 CMA patients, 6 CMA patients treated by epicutaneous therapy (CM-treated), and 4 CM-tolerant peanut allergic patients, we analyzed the IgE and IgG4 binding capacities related to major milk allergens in processed milk. Different proteases (plasmin, chymosin, α-chymotrypsin, or pepsin) were used progressively and selectively to hydrolyze β-lactoglobulin (β-LG) and casein (CN) in milk. We then showed that proteases differentially affect IgE or IgG4 immunoreactivities of CN and β-LG and also that we could not relate IgE and/or IgG4 levels or specificities to milk hydrolysates to the clinical status of the patients.
authors.affiliation: UMR Service de Pharmacologie et d'Immunoanalyse, CEA-INRA, Université Paris-Saclay , CEA de Saclay, F-91191 Gif-sur-Yvette, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Charcosset
authors.affiliation: UMR Service de Pharmacologie et d'Immunoanalyse, CEA-INRA, Université Paris-Saclay , CEA de Saclay, F-91191 Gif-sur-Yvette, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Adel-Patient
authors.affiliation: Pediatric Gastroenterology Service, Hôpital Necker Enfants Malades , F-75015 Paris, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Dupont
authors.affiliation: UMR Service de Pharmacologie et d'Immunoanalyse, CEA-INRA, Université Paris-Saclay , CEA de Saclay, F-91191 Gif-sur-Yvette, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Bernard
mesh_terms: D000818:Animals; D002417:Cattle; D002648:Child; D002675:Child, Preschool; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism*; D007074:Immunoglobulin G / Q000378:metabolism*; D007223:Infant; D008297:Male; D008894:Milk Proteins / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D007074:Immunoglobulin G; D008894:Milk Proteins; D007073:Immunoglobulin E; D010447:Peptide Hydrolases
keywords: IgE; IgG4; cow’s milk allergy; dairy proteases; gastrointestinal proteases; hydrolyzed proteins