title: Effects of Host Plant Factors on the Bacterial Communities Associated with Two Whitefly Sibling Species.
abstract: BACKGROUND
  Although discrepancy in the specific traits and ecological characteristics of Bemisia tabaci between species are partially attributed to the B. tabaci-associated bacteria, the factors that affect the diversity of B. tabaci-associated bacteria are not well-understood. We used the metagenomic approach to characterize the B. tabaci-associated bacterial community because the approach is an effective tool to identify the bacteria.
  
  
  RESULTS
  To investigate the effects of the host plant and a virus, tomato yellow leaf curl virus (TYLCV), on the bacterial communities of B. tabaci sibling species B and Q, we analyzed the bacterial communities associated with whitefly B and Q collected from healthy cotton, healthy tomato, and TYLCV-infected tomato. The analysis used miseq-based sequencing of a variable region of the bacterial 16S rDNA gene. For the bacteria associated with B. tabaci, we found that the influence of the host plant species was greater than that of the whitefly cryptic species. With further analysis of host plants infected with the TYLCV, the virus had no significant effects on the B. tabaci-associated bacterial community.
  
  
  CONCLUSIONS
  The effects of different plant hosts and TYLCV-infection on the diversity of B. tabaci-associated bacterial communities were successfully analyzed in this study. To explain why B. tabaci sibling species with different host ranges differ in performance, the analysis of the bacterial community may be essential to the explanation.
authors.affiliation: Key Lab of Integrated Crop Pest Management of Shandong Province, College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao, 266109, P. R. China.
authors.forename: Ming-Ming
authors.initials: MM
authors.lastname: Su
authors.affiliation: Key Lab of Integrated Crop Pest Management of Shandong Province, College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao, 266109, P. R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Guo
authors.affiliation: Key Lab of Integrated Crop Pest Management of Shandong Province, College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao, 266109, P. R. China.
authors.forename: Yun-Li
authors.initials: YL
authors.lastname: Tao
authors.affiliation: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China.
authors.forename: You-Jun
authors.initials: YJ
authors.lastname: Zhang
authors.affiliation: Key Lab of Integrated Crop Pest Management of Shandong Province, College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao, 266109, P. R. China.|State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100081, P.R. China.
authors.forename: Fang-Hao
authors.initials: FH
authors.lastname: Wan
authors.affiliation: Key Lab of Integrated Crop Pest Management of Shandong Province, College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao, 266109, P. R. China.
authors.forename: Dong
authors.initials: D
authors.lastname: Chu
mesh_terms: D000818:Animals; D003368:Gossypium / Q000382:microbiology; D006430:Hemiptera / Q000382:microbiology* / Q000502:physiology; D018551:Solanum lycopersicum / Q000382:microbiology; D056186:Metagenomics; D064307:Microbiota / Q000235:genetics / Q000502:physiology; D010942:Plant Viruses / Q000502:physiology