title: A Novel Conductive Poly(3,4-ethylenedioxythiophene)-BSA Film for the Construction of a Durable HRP Biosensor Modified with NanoAu Particles.
abstract: In this study, we have investigated the contribution of bovine serum albumin (BSA) to the durability of the electrochemically synthesized poly(3,4-ethylenedioxythiophene) (PEDOT) film on a platinum (Pt) electrode. The electrode was capable to effectively adsorb the nano Au particles (AuNPs) to form a uniform layout, which was then able to immobilize the horseradish peroxidase (HRP) to construct a functional HRP/AuNPs/PEDOT(BSA)/Pt biosensor. Cyclic voltammetry was employed to evaluate the performance of the biosensor through the measurement of hydrogen peroxide. Our results revealed a satisfied linear correlation between the cathodic current and the concentration of H2O2. Furthermore, the addition of oxidized form of nicotinamide adenine dinucleotide, or NAD⁺, as the electron transfer mediator in the detection solution could dramatically enhance the sensitivity of detection by about 35.5%. The main advantages of the current biosensor are its durability, sensitivity, reliability, and biocompatibility.
authors.affiliation: Department of Chemical and Biochemical Engineering, Xiamen University, No. 422, Siming South Road, Xiamen 361005, Fujian, China. fcxu@xmu.edu.cn.
authors.forename: Fangcheng
authors.initials: F
authors.lastname: Xu
authors.affiliation: Department of Chemical and Biochemical Engineering, Xiamen University, No. 422, Siming South Road, Xiamen 361005, Fujian, China. rht0517@126.com.|Department of Chemical and Materials Engineering, Tunghai University, No.1727, Sec.4, Taiwan Boulevard, Xitun District, Taichung 40704, Taiwan. rht0517@126.com.
authors.forename: Shuaibin
authors.initials: S
authors.lastname: Ren
authors.affiliation: Department of Chemical and Materials Engineering, Tunghai University, No.1727, Sec.4, Taiwan Boulevard, Xitun District, Taichung 40704, Taiwan. yegu@go.thu.edu.tw.
authors.forename: Yesong
authors.initials: Y
authors.lastname: Gu
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry; D002417:Cattle; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000302:isolation & purification*; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D004800:Enzymes, Immobilized; D011108:Polymers; C121383:poly(3,4-ethylene dioxythiophene); D012710:Serum Albumin, Bovine; D006046:Gold; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase
keywords: HRP biosensor; bovine serum albumin; electron transfer mediator; nano Au particle; poly(3,4-ethylenedioxythiophene)