title: Partially reduced graphene oxide based FRET on fiber-optic interferometer for biochemical detection.
abstract: Fluorescent resonance energy transfer (FRET) with naturally exceptional selectivity is a powerful technique and widely used in chemical and biomedical analysis. However, it is still challenging for conventional FRET to perform as a high sensitivity compact sensor. Here we propose a novel 'FRET on Fiber' concept, in which a partially reduced graphene oxide (prGO) film is deposited on a fiber-optic modal interferometer, acting as both the fluorescent quencher for the FRET and the sensitive cladding for optical phase measurement due to refractive index changes in biochemical detection. The target analytes induced fluorescence recovery with good selectivity and optical phase shift with high sensitivity are measured simultaneously. The functionalized prGO film coated on the fiber-optic interferometer shows high sensitivities for the detections of metal ion, dopamine and single-stranded DNA (ssDNA), with detection limits of 1.2 nM, 1.3 μM and 1 pM, respectively. Such a prGO based 'FRET on fiber' configuration, bridging the FRET and the fiber-optic sensing technology, may serve as a platform for the realization of series of integrated 'FRET on Fiber' sensors for on-line environmental, chemical, and biomedical detection, with excellent compactness, high sensitivity, good selectivity and fast response.
authors.affiliation: Key Laboratory of Optical Fiber Sensing and Communications (Education Ministry of China), University of Electronic Science and Technology of China, Chengdu 610054, China.
authors.forename: B C
authors.initials: BC
authors.lastname: Yao
authors.affiliation: Key Laboratory of Optical Fiber Sensing and Communications (Education Ministry of China), University of Electronic Science and Technology of China, Chengdu 610054, China.|Center for Information in BioMedicine, University of Electronic Science and Technology of China, Chengdu 611731, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Key Laboratory of Optical Fiber Sensing and Communications (Education Ministry of China), University of Electronic Science and Technology of China, Chengdu 610054, China.
authors.forename: C B
authors.initials: CB
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Electronic Thin Films and Integrated Devices, University of Electronic Science and Technology of China, Chengdu 610054, China.
authors.forename: J R
authors.initials: JR
authors.lastname: He
authors.affiliation: Key Laboratory of Optical Fiber Sensing and Communications (Education Ministry of China), University of Electronic Science and Technology of China, Chengdu 610054, China.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Rao
authors.affiliation: Key Laboratory of Optical Fiber Sensing and Communications (Education Ministry of China), University of Electronic Science and Technology of China, Chengdu 610054, China.|Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, United States.|Center for Information in BioMedicine, University of Electronic Science and Technology of China, Chengdu 611731, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Gong
authors.affiliation: State Key Laboratory of Electronic Thin Films and Integrated Devices, University of Electronic Science and Technology of China, Chengdu 610054, China.
authors.forename: F
authors.initials: F
authors.lastname: Fu
authors.affiliation: State Key Laboratory of Electronic Thin Films and Integrated Devices, University of Electronic Science and Technology of China, Chengdu 610054, China.
authors.forename: Y F
authors.initials: YF
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Electronic Thin Films and Integrated Devices, University of Electronic Science and Technology of China, Chengdu 610054, China.
authors.forename: Y R
authors.initials: YR
authors.lastname: Li
mesh_terms: D015374:Biosensing Techniques; D005336:Fiber Optic Technology; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D010087:Oxides / Q000737:chemistry
chemical_list: D010087:Oxides; D006108:Graphite