title: A Mammalian enhancer trap resource for discovering and manipulating neuronal cell types.
abstract: There is a continuing need for driver strains to enable cell-type-specific manipulation in the nervous system. Each cell type expresses a unique set of genes, and recapitulating expression of marker genes by BAC transgenesis or knock-in has generated useful transgenic mouse lines. However, since genes are often expressed in many cell types, many of these lines have relatively broad expression patterns. We report an alternative transgenic approach capturing distal enhancers for more focused expression. We identified an enhancer trap probe often producing restricted reporter expression and developed efficient enhancer trap screening with the PiggyBac transposon. We established more than 200 lines and found many lines that label small subsets of neurons in brain substructures, including known and novel cell types. Images and other information about each line are available online (enhancertrap.bio.brandeis.edu).
authors.affiliation: Department of Biology and National Center for Behavioral Genomics, Brandeis University, Waltham, United States.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Shima
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
authors.forename: Ken
authors.initials: K
authors.lastname: Sugino
authors.affiliation: Department of Biology and National Center for Behavioral Genomics, Brandeis University, Waltham, United States.
authors.forename: Chris Martin
authors.initials: CM
authors.lastname: Hempel
authors.affiliation: Department of Biology and National Center for Behavioral Genomics, Brandeis University, Waltham, United States.
authors.forename: Masami
authors.initials: M
authors.lastname: Shima
authors.affiliation: Department of Biology and National Center for Behavioral Genomics, Brandeis University, Waltham, United States.
authors.forename: Praveen
authors.initials: P
authors.lastname: Taneja
authors.affiliation: Department of Biology and National Center for Behavioral Genomics, Brandeis University, Waltham, United States.
authors.forename: James B
authors.initials: JB
authors.lastname: Bullis
authors.affiliation: Department of Biology and National Center for Behavioral Genomics, Brandeis University, Waltham, United States.
authors.forename: Sonam
authors.initials: S
authors.lastname: Mehta
authors.affiliation: Division of Biology and Biological Engineering, Beckman Institute, California Institute of Technology, Pasadena, United States.
authors.forename: Carlos
authors.initials: C
authors.lastname: Lois
authors.affiliation: Department of Biology and National Center for Behavioral Genomics, Brandeis University, Waltham, United States.
authors.forename: Sacha B
authors.identifier: http://orcid.org/0000-0002-0108-8599
authors.initials: SB
authors.lastname: Nelson
mesh_terms: D000818:Animals; D051379:Mice; D008822:Mice, Transgenic; D008967:Molecular Biology / Q000379:methods*; D009446:Neurobiology / Q000379:methods*; D009474:Neurons / Q000502:physiology*; D013194:Staining and Labeling / Q000379:methods*
keywords: cell type; enhancer trap; mouse; neuroscience; transgenic animals