title: Alcohol produces distinct hepatic lipidome and eicosanoid signature in lean and obese.
abstract: Alcohol- and obesity-related liver diseases often coexist. The hepatic lipidomics due to alcohol and obesity interaction is unknown. We characterized the hepatic lipidome due to 1) alcohol consumption in lean and obese mice and 2) obesity and alcohol interactions. In the French-Tsukamoto mouse model, intragastric alcohol or isocaloric dextrose were fed with either chow (lean) or high-fat, high-cholesterol diet (obese). Four groups (lean, lean alcohol, obese, and obese alcohol) were studied. MS was performed for hepatic lipidomics, and data were analyzed. Alcohol significantly increased hepatic cholesteryl esters and diacyl-glycerol in lean and obese but was more pronounced in obese. Alcohol produced contrasting changes in hepatic phospholipids with significant enrichment in lean mice versus significant decrease in obese mice, except phosphatidylglycerol, which was increased in both lean and obese alcohol groups. Most lysophospholipids were increased in lean alcohol and obese mice without alcohol use only. Prostaglandin E2; 5-, 8-, and 11-hydroxyeicosatetraenoic acids; and 9- and 13-hydroxyoctadecadienoic acids were considerably increased in obese mice with alcohol use. Alcohol consumption produced distinct changes in lean and obese with profound effects of obesity and alcohol interaction on proinflammatory and oxidative stress-related eicosanoids.
authors.affiliation: Division of Gastroenterology, Hepatology and Nutrition, Department of Internal Medicine, Virginia Commonwealth University School of Medicine, Richmond, VA puneet.puri@vcuhealth.org.
authors.forename: Puneet
authors.initials: P
authors.lastname: Puri
authors.affiliation: University of Southern California, Los Angeles, CA.
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.affiliation: Zora Biosciences Oy, Espoo, Finland.
authors.forename: Terhi
authors.initials: T
authors.lastname: Vihervaara
authors.affiliation: Zora Biosciences Oy, Espoo, Finland.
authors.forename: Riikka
authors.initials: R
authors.lastname: Katainen
authors.affiliation: Zora Biosciences Oy, Espoo, Finland.
authors.forename: Kim
authors.initials: K
authors.lastname: Ekroos
authors.affiliation: Division of Gastroenterology, Hepatology and Nutrition, Department of Internal Medicine, Virginia Commonwealth University School of Medicine, Richmond, VA.
authors.forename: Kalyani
authors.initials: K
authors.lastname: Daita
authors.affiliation: Division of Gastroenterology, Hepatology and Nutrition, Department of Internal Medicine, Virginia Commonwealth University School of Medicine, Richmond, VA.
authors.forename: Hae-Ki
authors.initials: HK
authors.lastname: Min
authors.affiliation: Venebio Group LLC, Richmond, VA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Joyce
authors.affiliation: Division of Gastroenterology, Hepatology and Nutrition, Department of Internal Medicine, Virginia Commonwealth University School of Medicine, Richmond, VA.
authors.forename: Faridoddin
authors.initials: F
authors.lastname: Mirshahi
authors.affiliation: University of Southern California, Los Angeles, CA.
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Tsukamoto
authors.affiliation: Division of Gastroenterology, Hepatology and Nutrition, Department of Internal Medicine, Virginia Commonwealth University School of Medicine, Richmond, VA.
authors.forename: Arun J
authors.initials: AJ
authors.lastname: Sanyal
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism / Q000473:pathology; D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D059305:Diet, High-Fat; D015777:Eicosanoids / Q000235:genetics* / Q000378:metabolism; D000431:Ethanol / Q000633:toxicity; D050356:Lipid Metabolism / Q000187:drug effects / Q000235:genetics*; D008099:Liver / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008107:Liver Diseases / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D008820:Mice, Obese; D009765:Obesity / Q000378:metabolism* / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D014280:Triglycerides / Q000378:metabolism
chemical_list: D015777:Eicosanoids; D014280:Triglycerides; D000431:Ethanol; D002784:Cholesterol
keywords: cyclooxygenase; cytochrome P450; hydroxyeicosatetraenoic acids; lipoxygenase; liver; obesity; phospholipids; prostaglandins