title: NINA-LAMP compared to microscopy, RDT, and nested PCR for the detection of imported malaria.
abstract: Microscopy and field adaptable rapid diagnostic tests (RDTs) are not sensitive and specific in certain conditions such as poor training of microscopists, lack of electricity, or lower sensitivity in the detection of non-falciparum malaria. More sensitive point-of-care testing (POCT) would reduce delays in diagnosis and initiation of therapy. In the current study, we have evaluated the efficacy of noninstrumented nucleic acid amplification (NINA) coupled with loop-mediated isothermal amplification (LAMP) for detection of traveler's malaria (n=140) in comparison with microscopy, nested PCR, and the only Food and Drug Administration-approved rapid diagnostic test. NINA-LAMP was 100% sensitive and 98.6% specific when compared to nested PCR. For non-falciparum detection, NINA-LAMP sensitivity was 100% sensitive compared to nested PCR, whereas RDT sensitivity was 71%. LAMP is highly sensitive and specific for symptomatic malaria diagnosis regardless of species.
authors.affiliation: Department of Microbiology, Immunology and Infectious Disease, University of Calgary, AB, Canada; Department of Pathology and Laboratory Medicine, University of Calgary, AB, Canada.
authors.forename: Abu Naser
authors.initials: AN
authors.lastname: Mohon
authors.affiliation: Department of Microbiology, Immunology and Infectious Disease, University of Calgary, AB, Canada.
authors.forename: Lydia Da-Yeong
authors.initials: LD
authors.lastname: Lee
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Calgary, AB, Canada.
authors.forename: Abebe Genetu
authors.initials: AG
authors.lastname: Bayih
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Calgary, AB, Canada.
authors.forename: Asongna
authors.initials: A
authors.lastname: Folefoc
authors.affiliation: PATH, Seattle, WA, USA.
authors.forename: Dylan
authors.initials: D
authors.lastname: Guelig
authors.affiliation: PATH, Seattle, WA, USA.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Burton
authors.affiliation: PATH, Seattle, WA, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: LaBarre
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Calgary, AB, Canada.
authors.forename: Wilson
authors.initials: W
authors.lastname: Chan
authors.affiliation: Department of Medicine, University of Calgary, Calgary, AB, Canada.
authors.forename: Bonnie
authors.initials: B
authors.lastname: Meatherall
authors.affiliation: Department of Microbiology, Immunology and Infectious Disease, University of Calgary, AB, Canada; Department of Pathology and Laboratory Medicine, University of Calgary, AB, Canada; Department of Medicine, University of Calgary, Calgary, AB, Canada. Electronic address: drpillai@ucalgary.ca.
authors.forename: Dylan R
authors.initials: DR
authors.lastname: Pillai
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D003955:Diagnostic Tests, Routine / Q000379:methods*; D006801:Humans; D008288:Malaria / Q000175:diagnosis*; D008853:Microscopy / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D019095:Point-of-Care Systems; D012680:Sensitivity and Specificity; D014195:Travel
keywords: LAMP; Malaria; Point of care test