title: Characterization and anti-biofilm activity of extracellular polymeric substances produced by the marine biofilm-forming bacterium Pseudoalteromonas ulvae strain TC14.
abstract: This study investigated soluble (Sol-EPS), loosely bound (LB-EPS), and tightly bound extracellular polymeric substances (TB-EPS) harvested from biofilm and planktonic cultures of the marine bacterium Pseudoalteromonas ulvae TC14. The aim of the characterization (colorimetric methods, FTIR, GC-MS, NMR, HPGPC, and AFM analyses) was to identify new anti-biofilm compounds; activity was assessed using the BioFilm Ring Test®. A step-wise separation of EPS was designed, based on differences in water-solubility and acidity. An acidic fraction was isolated from TB-EPS, which strongly inhibited biofilm formation by marine bacterial strains in a concentration-dependent manner. The main constituents of this fraction were characterized as two glucan-like polysaccharides. An active poly(glutamyl-glutamate) fraction was also recovered from TB-EPS. The distribution of these key EPS components in Sol-EPS, LB-EPS, and TB-EPS was distinct and differed quantitatively in biofilm vs planktonic cultures. The anti-biofilm potential of the fractions emphasizes the putative antifouling role of EPS in the environment.
authors.affiliation: a Laboratoire MAPIEM, EA 4323 , Université de Toulon , La Garde , France.
authors.forename: Florence
authors.initials: F
authors.lastname: Brian-Jaisson
authors.affiliation: a Laboratoire MAPIEM, EA 4323 , Université de Toulon , La Garde , France.
authors.forename: Maëlle
authors.initials: M
authors.lastname: Molmeret
authors.affiliation: a Laboratoire MAPIEM, EA 4323 , Université de Toulon , La Garde , France.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Fahs
authors.affiliation: a Laboratoire MAPIEM, EA 4323 , Université de Toulon , La Garde , France.
authors.forename: Linda
authors.initials: L
authors.lastname: Guentas-Dombrowsky
authors.affiliation: a Laboratoire MAPIEM, EA 4323 , Université de Toulon , La Garde , France.
authors.forename: Gérald
authors.initials: G
authors.lastname: Culioli
authors.affiliation: a Laboratoire MAPIEM, EA 4323 , Université de Toulon , La Garde , France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Blache
authors.affiliation: b Service commun de Résonance Magnétique Nucléaire , Université de Bretagne Occidentale , Brest , France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Cérantola
authors.affiliation: a Laboratoire MAPIEM, EA 4323 , Université de Toulon , La Garde , France.
authors.forename: Annick
authors.initials: A
authors.lastname: Ortalo-Magné
mesh_terms: D018441:Biofilms / Q000187:drug effects*; D018625:Microscopy, Atomic Force; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D031161:Pseudoalteromonas / Q000378:metabolism*; D014871:Water Microbiology
chemical_list: D011135:Polysaccharides, Bacterial
keywords: EPS; Pseudoalteromonas ulvae; anti-biofilm; glucan; marine bacteria; poly(glutamyl-glutamate)