title: CD133 does not enrich for the stem cell activity in vivo in adult mouse prostates.
abstract: CD133 is widely used as a marker for stem/progenitor cells in many organ systems. Previous studies using in vitro stem cell assays have suggested that the CD133-expressing prostate basal cells may serve as the putative prostate stem cells. However, the precise localization of the CD133-expressing cells and their contributions to adult murine prostate homeostasis in vivo remain undetermined. We show that loss of function of CD133 does not impair murine prostate morphogenesis, homeostasis and regeneration, implying a dispensable role for CD133 in prostate stem cell function. Using a CD133-CreER(T2) model in conjunction with a fluorescent report line, we show that CD133 is not only expressed in a fraction of prostate basal cells, but also in some luminal cells and stromal cells. CD133(+) basal cells possess higher in vitro sphere-forming activities than CD133(-) basal cells. However, the in vivo lineage tracing study reveals that the two cell populations possess the same regenerative capacity and contribute equally to the maintenance of the basal cell lineage. Similarly, CD133(+) and CD133(-) luminal cells are functionally equivalent in maintaining the luminal cell lineage. Collectively, our study demonstrates that CD133 does not enrich for the stem cell activity in vivo in adult murine prostate. This study does not contradict previous reports showing CD133(+) cells as prostate stem cells in vitro. Instead, it highlights a substantial impact of biological contexts on cellular behaviors.
authors.affiliation: Department of Molecular and Cellular Biology, Baylor College of Medicine, United States; Graduate Program in Integrative Molecular and Biomedical Sciences, Baylor College of Medicine, United States.
authors.forename: Xing
authors.initials: X
authors.lastname: Wei
authors.affiliation: Biological Technologies, Analytical Development & Quality Control, Genentech Inc., United States.
authors.forename: Arturo V
authors.initials: AV
authors.lastname: Orjalo
authors.affiliation: Department of Molecular and Cellular Biology, Baylor College of Medicine, United States; Department of Pathology and Immunology, United States; Dan L. Duncan Cancer Center, Baylor College of Medicine, United States. Electronic address: xin@bcm.edu.
authors.forename: Li
authors.initials: L
authors.lastname: Xin
mesh_terms: D000071916:AC133 Antigen / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D004848:Epithelium / Q000502:physiology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011467:Prostate / Q000166:cytology / Q000378:metabolism*; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism; D012038:Regeneration; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D000071916:AC133 Antigen; C000606023:PROM1 protein, human; D012313:RNA
keywords: CD133; Lineage tracing; Prostate stem cells