title: In silico study on Arabidopsis BAG gene expression in response to environmental stresses.
abstract: BAG (Bcl-2 athanogene) family proteins are conserved in a wide range of eukaryotes, and they have been proposed to play a crucial role in plant programmed cell death (PCD). During the past decade, with the help of advanced bioinformatics tools, seven homologs of BAG genes have been identified in the Arabidopsis genome; these genes are involved in pathogen attack and abiotic stress conditions. In this study, gene expression of Arabidopsis BAG family members under environmental stresses was analyzed using the Botany Array Resource (BAR) expression browser tool and the in silico data were partially confirmed by qRT-PCR analysis for the selected stress- and hormone-treated conditions related to environmental stresses. Particularly, the induction of AtBAG6 gene in response to heat shock was confirmed by using GUS reporter lines. The loss of the AtBAG6 gene resulted into impairment in basal thermotolerance of plant and showed enhanced cell death in response to heat stress. To elucidate the regulatory mechanisms of BAG genes, we analyzed ∼1-kbp promoter regions for the presence of stress-responsive elements. Our transcription profiling finally revealed that the Arabidopsis BAG genes differentially respond to environmental stresses under the control of specifically organized upstream regulatory elements.
authors.affiliation: Division of Applied Life Science and PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju, 52828, Republic of Korea.
authors.forename: Ganesh M
authors.initials: GM
authors.lastname: Nawkar
authors.affiliation: Division of Applied Life Science and PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju, 52828, Republic of Korea.
authors.forename: Punyakishore
authors.initials: P
authors.lastname: Maibam
authors.affiliation: Division of Applied Life Science and PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju, 52828, Republic of Korea.
authors.forename: Joung Hun
authors.initials: JH
authors.lastname: Park
authors.affiliation: Eco-friendly Bio-Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, 580-185, Republic of Korea.
authors.forename: Su Gyeong
authors.initials: SG
authors.lastname: Woo
authors.affiliation: Eco-friendly Bio-Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, 580-185, Republic of Korea.
authors.forename: Cha Young
authors.initials: CY
authors.lastname: Kim
authors.affiliation: Division of Applied Life Science and PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju, 52828, Republic of Korea. sylee@gnu.ac.kr.
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Division of Applied Life Science and PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju, 52828, Republic of Korea. jacobgnu69@gnu.ac.kr.
authors.forename: Chang Ho
authors.initials: CH
authors.lastname: Kang
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003198:Computer Simulation; D004777:Environment; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D017930:Genes, Reporter; D005966:Glucuronidase / Q000378:metabolism; D018869:Heat-Shock Response / Q000187:drug effects / Q000235:genetics; D059372:Nucleotide Motifs / Q000235:genetics; D010937:Plant Growth Regulators / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics*; D000071436:Thermotolerance / Q000187:drug effects / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D010937:Plant Growth Regulators; D012333:RNA, Messenger; D005966:Glucuronidase
keywords: Bcl-2 athanogene (BAG); Environmental stress; In silico data; Stress-responsive elements; Transcription profiling