title: Structural Analysis of the Lipopeptide Produced by the Bacillus subtilis Mutant R2-104 with Mutagenesis.
abstract: The lipopeptide and its homologues are a kind of the five major biosurfactants with prominent interfacial and biological activities. A suite of mutagenesis method was adopted to expose a wild lipopeptide-producing strain Bacillus subtilis HSO121 to improve lipopeptide yield, and a stable mutant named R2-104 with a 2.0-fold production of lipopeptide was obtained. Compared to that of the wild strain HSO121, the lipopeptide produced by R2-104 showed a similar surface activity, but the course profiles of lipopeptide production during cultivation were different, with the peak yield of 500 mg at about 9 h by R2-104, and 400 mg at about 5 h by HSO121. The constituent abundance of the lipopeptide homologues produced by R2-104 was also different from that by HSO121. Combined methods of ESI-MS, GC-MS and MS-MS were applied for structural characterization of lipopeptide homologues, and it showed that the lipopeptides produced by R2-104 and HSO121 were attributed to a surfactin family with different constituents. The dominant constituent of the surfactin family produced by R2-104 was anteiso C15-surfactin with a relative content of 43.8 %, while the dominant one produced by HSO121was iso C14-surfactin with a relative content of 33.1 %.
authors.affiliation: State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Meng
authors.affiliation: State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
authors.forename: Jia
authors.initials: J
authors.lastname: You
authors.affiliation: State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
authors.forename: Hong-Ze
authors.initials: HZ
authors.lastname: Gang
authors.affiliation: State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
authors.forename: Jin-Feng
authors.initials: JF
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
authors.forename: Shi-Zhong
authors.initials: SZ
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
authors.forename: Ru-Qiang
authors.initials: RQ
authors.lastname: Ye
authors.affiliation: State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai, 200237, People's Republic of China. bzmu@ecust.edu.cn.
authors.forename: Bo-Zhong
authors.initials: BZ
authors.lastname: Mu
mesh_terms: D001412:Bacillus subtilis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D055666:Lipopeptides / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D016296:Mutagenesis; D050505:Mutant Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism; D013501:Surface-Active Agents / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D055666:Lipopeptides; D050505:Mutant Proteins; D013501:Surface-Active Agents
keywords: Bacillus subtilis; Lipopeptide; Mutagenesis; Structural analysis; Surfactin