title: Poly (ADP-ribose) polymerase1 regulates growth and multicellularity in D. discoideum.
abstract: Poly (ADP-ribose) polymerase (PARP)-1 regulates various biological processes like DNA repair, cell death etc. However, the role of PARP-1 in growth and differentiation still remains elusive. The present study has been undertaken to understand the role of PARP-1 in growth and development of a unicellular eukaryote, Dictyostelium discoideum. In silico analysis demonstrates ADPRT1A as the ortholog of human PARP-1 in D. discoideum. The present study shows that ADPRT1A overexpression (A OE) led to slow growth of D. discoideum and significant population of AOE cells were in S and G2/M phase. Also, AOE cells exhibited high endogenous PARP activity, significant NAD(+) depletion and also significantly lower ADPRT1B and ADPRT2 transcript levels. Moreover, AOE cells are intrinsically stressed and also exhibited susceptibility to oxidative stress. AOE also affected development of D. discoideum predominantly streaming, aggregation and formation of early culminant which are concomitant with reports on PARP's role in D. discoideum development. In addition, under developmental stimuli, increased PARP activity was seen along with developmentally regulated transcript levels of ADPRT1A during D. discoideum multicellularity. Thus the present study suggests that PARP-1 regulates growth as well as the developmental morphogenesis of D. discoideum, thereby opening new avenues to understand the same in higher eukaryotes.
authors.affiliation: Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat 390002, India. Electronic address: alex.tina1901@gmail.com.
authors.forename: Tina
authors.initials: T
authors.lastname: Jubin
authors.affiliation: Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat 390002, India. Electronic address: ashleshakadam@rediffmail.com.
authors.forename: Ashlesha
authors.initials: A
authors.lastname: Kadam
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India. Electronic address: ssaran@mail.jnu.ac.in.
authors.forename: Shweta
authors.initials: S
authors.lastname: Saran
authors.affiliation: Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat 390002, India. Electronic address: rasheedunnisab@yahoo.co.in.
authors.forename: Rasheedunnisa
authors.initials: R
authors.lastname: Begum
mesh_terms: D036002:ADP Ribose Transferases / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D002453:Cell Cycle; D002454:Cell Differentiation; D002478:Cells, Cultured; D004023:Dictyostelium / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D053078:Membrane Potential, Mitochondrial; D018384:Oxidative Stress; D010802:Phylogeny; D000071137:Poly (ADP-Ribose) Polymerase-1 / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: D012333:RNA, Messenger; D036002:ADP Ribose Transferases; D000071137:Poly (ADP-Ribose) Polymerase-1
keywords: ADPRT1A; Cell cycle; Cell death; D. discoideum; Development; Overexpression