title: Studies on the relationship between the point mutation of ras oncogenes and the prognosis of patients with gastric cancer.
abstract: OBJECTIVE
  To study the relationship between the point mutation of ras oncogenes and the prognosis of patients with gastric cancer.
  
  
  METHODS
  The point mutations at codon 12 and 61 of c-Ha-ras, at codon 12 and 13 of K-ras, and at codon 12 of N-ras were studied with PCR-RFLP in 88 formalin fixed and paraffin embedded specimens of gastric cancer.
  
  
  RESULTS
  It was found that the overall rate of point mutation of ras oncogenes was 18.2% and the positivity of the point mutation of ras oncogenes was related to the cancerous invasion of the serosa, the status of lymph node metastasis, the stage of cancer and the survival time after surgery.
  
  
  CONCLUSIONS
  The findings suggest that the determination of point mutations of ras oncogenes can be used to determine the prognosis of patients with gastric cancer.
authors.affiliation: Dian-Chun Fang, Yuan-Hui Luo, Rong Lu, Wei-Wen Liu, Department of Gastroentrology, Southwest Hospital, Third Military Medical University, Chongqing 630038, China.
authors.forename: D C
authors.initials: DC
authors.lastname: Fang
authors.affiliation: Dian-Chun Fang, Yuan-Hui Luo, Rong Lu, Wei-Wen Liu, Department of Gastroentrology, Southwest Hospital, Third Military Medical University, Chongqing 630038, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Luo
authors.affiliation: Dian-Chun Fang, Yuan-Hui Luo, Rong Lu, Wei-Wen Liu, Department of Gastroentrology, Southwest Hospital, Third Military Medical University, Chongqing 630038, China.
authors.forename: R
authors.initials: R
authors.lastname: Lu
authors.affiliation: Dian-Chun Fang, Yuan-Hui Luo, Rong Lu, Wei-Wen Liu, Department of Gastroentrology, Southwest Hospital, Third Military Medical University, Chongqing 630038, China.
authors.forename: W W
authors.initials: WW
authors.lastname: Liu
keywords: Genes; Polymerase chain reaction; Polymorphism: Restriction fragment length; Prognosis; Ras point mutation; Stomach neoplasms