title: Synergistic antioxidant activity of resveratrol with genistein in high-glucose treated Madin-Darby canine kidney epithelial cells.
abstract: Resveratrol (Re), a stilbenoid, is associated with a potential benefit in controlling certain biomarkers in type II diabetes. Genistein (Ge), a phytoestrogen, may act as an antioxidant and thus may diminish damaging effects of free radicals in tissues. In the present study, a potential synergistic antioxidant effect of an Re/Ge combination on high-glucose (HG) incubation in Madin-Darby canine kidney (MDCK) epithelial cells was evaluated. Compared with the treatment of Re or Ge alone, the Re/Ge combination synergistically decreased intracellular reactive oxygen species (ROS) and hydroxyl radicals in MDCK cells. This synergistic antioxidant effect correlated with the inhibition of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase expression and an increase in γ-glutamylcysteine synthetase expression. In addition, mitochondrial complex I, NADPH oxidase, xanthine oxidase and lipoxygenase contributed towards ROS overproduction when the MDCK cells were incubated with HG. In conclusion, the Re/Ge combination synergistically enhanced the antioxidant effect in HG-incubated kidney cells, possibly through an enhanced antioxidant regulation mechanism. The Re/Ge combination may be a potential benefit against oxidative stress in diabetes mellitus.
authors.affiliation: Department of Microbiology, Immunology and Biopharmaceuticals, College of Life Sciences, National Chiayi University, Chiayi 60004, Taiwan, R.O.C.
authors.forename: Chishih
authors.initials: C
authors.lastname: Chu
authors.affiliation: Institute of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan, R.O.C.
authors.forename: Fung-Jou
authors.initials: FJ
authors.lastname: Lu
authors.affiliation: Department of Physical Medicine and Rehabilitation, Chia-Yi Chang Gung Memorial Hospital, Chiayi 61363, Taiwan, R.O.C.
authors.forename: Rang-Hui
authors.initials: RH
authors.lastname: Yeh
authors.affiliation: Department of Microbiology, Immunology and Biopharmaceuticals, College of Life Sciences, National Chiayi University, Chiayi 60004, Taiwan, R.O.C.
authors.forename: Zih-Ling
authors.initials: ZL
authors.lastname: Li
authors.affiliation: Department of Microbiology, Immunology and Biopharmaceuticals, College of Life Sciences, National Chiayi University, Chiayi 60004, Taiwan, R.O.C.
authors.forename: Ching-Hsein
authors.initials: CH
authors.lastname: Chen
keywords: Madin-Darby canine kidney cells; antioxidant; genistein; reactive oxygen species; resveratrol