title: The role of the tissue plasminogen activator as a prognostic and differentiation factor in patients with pancreatic cancer and chronic pancreatitis.
abstract: Unsatisfactory pancreatic cancer treatment outcomes have prompted multiple avenues of research focused on identifying not only biomarkers of pancreatic adenocarcinoma progression but also potential prognostic survival factors in patients with pancreatic adenocarcinoma. Study consisted of 75 patients who underwent pancreatic resections between 2006 and 2011: 35 patients with pancreatic ductal adenocarcinoma (PC), 30 patients with chronic pancreatitis (CP), and a non-malignant control group (NMCG) of 10 patients who underwent surgery due to benign tumors. Tissue plasminogen activator (t-PA) concentrations in tissue homogenates and sera were evaluated. The mean t-PA concentration in PC tissue homogenates was 12.3 ± 2 (7.5, 15) ng/mg. Compared with the t-PA concentration in the PC group, lower concentrations of t-PA (3.3 ± 0.7 (2.2, 4.7) ng/mg and 5.9 ± 0.8 (4.6, 7.3) ng/mg (P < 0.01)) were observed in tissue homogenates of the CP and the NMCG patients, respectively. Although serum concentrations of t-PA did not differ between patient groups, in PC patients, the t-PA concentrations were higher in sera than in tissue homogenates. In contrast, the CP and NMCG patient groups had lower t-PA concentrations in sera compared with tissue homogenates. Increasing tissue homogenate t-PA concentrations were associated with blood vessels infiltration. Tissue homogenate and serum t-PA concentrations were not related to the survival rate of patients with PC. The t-PA concentration above 7.45 ng/ml in tissue homogenates was indicative of PC. We concluded that higher concentrations of t-PA were observed in pancreatic cancer tissue compared to chronic pancreatitis, suggesting its potential role in the development and progression of pancreatic cancer. In contrast, the lack of significant differences in the serum t-PA concentrations between treatment groups suggests that serum t-PA concentrations may not be suitable as a biomarker for the diagnosis of pancreatic cancer.
authors.affiliation: Department of Gastrointestinal Surgery, Medical University of Silesia, Katowice, Poland.
authors.forename: D
authors.initials: D
authors.lastname: Baluka
authors.affiliation: Department of General and Vascular Surgery, Medical University of Silesia, Katowice, Poland.
authors.forename: T
authors.initials: T
authors.lastname: Urbanek
authors.affiliation: Department of Gastrointestinal Surgery, Medical University of Silesia, Katowice, Poland. andrzejlekstan@gmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Lekstan
authors.affiliation: Department of Biochemistry, Medical University of Silesia, Zabrze Rokitnica, Poland.
authors.forename: E
authors.initials: E
authors.lastname: Swietochowska
authors.affiliation: Department of Histology and Embryology, Medical University of Silesia, Katowice, Poland.
authors.forename: R
authors.initials: R
authors.lastname: Wiaderkiewicz
authors.affiliation: Department of Pathomorphology, Medical University of Silesia, Katowice, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Kajor
authors.affiliation: Department of Physiology, Medical University of Silesia, Katowice, Poland.
authors.forename: H
authors.initials: H
authors.lastname: Jedrzejewska-Szypulka
authors.affiliation: Department of Gastrointestinal Surgery, Medical University of Silesia, Katowice, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Kusnierz
authors.affiliation: Department of Gastrointestinal Surgery, Medical University of Silesia, Katowice, Poland.
authors.forename: P
authors.initials: P
authors.lastname: Lampe
mesh_terms: D000230:Adenocarcinoma / Q000097:blood / Q000378:metabolism / Q000473:pathology; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D021441:Carcinoma, Pancreatic Ductal / Q000097:blood / Q000378:metabolism / Q000473:pathology; D002454:Cell Differentiation / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000097:blood / Q000378:metabolism / Q000473:pathology*; D050500:Pancreatitis, Chronic / Q000097:blood / Q000378:metabolism / Q000473:pathology*; D011379:Prognosis; D010959:Tissue Plasminogen Activator / Q000097:blood* / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D010959:Tissue Plasminogen Activator