title: Single-Cell Analysis Using Drop-on-Demand Inkjet Printing and Probe Electrospray Ionization Mass Spectrometry.
abstract: This study describes a novel method for single-cell analysis and lipid profiling by combining drop-on-demand inkjet cell printing and probe electrospray ionization mass spectrometry (PESI-MS). Through inkjet sampling of a cell suspension, droplets with single cells were generated, precisely dripped onto a tungsten-made electrospray ionization needle, and immediately sprayed under a high-voltage electric field. Lipid fingerprints of single cells were obtained by a mass spectrometry (MS) detector. A homemade magnetic stirring device was applied to the cell suspension reservoir, which controlled the homogeneous distribution of cells in liquid and improved the single-cell-droplet percentage by 43.8%. Eight types of single cells were screened in our platform and further differentiated by principal component analysis based on cellular surface phospholipids. Thus, this study successfully provides a facile method for the direct MS profiling of single-cell lipids by PESI-MS.
authors.affiliation: Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University , Beijing 100084, China.
authors.forename: Fengming
authors.initials: F
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University , Beijing 100084, China.
authors.forename: Luyao
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University , Beijing 100084, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University , Beijing 100084, China.
authors.forename: Ziyi
authors.initials: Z
authors.lastname: He
authors.affiliation: Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University , Minamiohsawa, Hachioji, Tokyo 192-0397, Japan.
authors.forename: Katsumi
authors.initials: K
authors.lastname: Uchiyama
authors.affiliation: Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University , Beijing 100084, China.|Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in University of Shandong, Shandong Normal University , Jinan 250014, China.
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Lin
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D051379:Mice; D015335:Molecular Probes; D010316:Particle Size; D011327:Printing; D059010:Single-Cell Analysis / Q000295:instrumentation* / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation*; D014414:Tungsten
chemical_list: D015335:Molecular Probes; D014414:Tungsten