title: Characterization of microsatellite markers for Baccharis dracunculifolia (Asteraceae).
abstract: OBJECTIVE
  Baccharis dracunculifolia (Asteraceae) is a native plant of the Atlantic Forest that is used for the production of essential oil. Microsatellite markers were developed for this species to investigate the genetic diversity of three natural populations.
  
  
  RESULTS
  Seventeen out of 27 microsatellite loci identified in a genomic library used for the characterization of 315 individuals derived from three natural populations of B. dracunculifolia resulted in successful amplifications. Eleven polymorphic loci, ranging from two to seven alleles per locus, were obtained with expected and observed heterozygosity values ranging between 0.068 and 0.775 and 0.046 and 0.667, respectively.
  
  
  CONCLUSIONS
  The microsatellite loci described in this study are tools that can be used for further studies of population genetics of B. dracunculifolia with a focus on deforested areas and conservation of natural populations.
authors.affiliation: Programa de Pós-graduação em Horticultura, Universidade Estadual Paulista, Rua José Barbosa de Barros 1780, 18610-307 Botucatu, São Paulo, Brazil.
authors.forename: Camila M B
authors.initials: CM
authors.lastname: Belini
authors.affiliation: Departamento de Fitoquímica, Instituto Agronômico de Campinas, Avenida Doutor Theodureto de Almeida Camargo 1500, 13012-970 Campinas, São Paulo, Brazil.
authors.forename: Marcia O M
authors.initials: MO
authors.lastname: Marques
authors.affiliation: Centro Pluridisciplinar de Pesquisas Químicas Biológicas e Agrícolas, Universidade Estadual de Campinas, CP 6171, Rua Alexandre Cazelatto 999, 13081-970 Paulínia, São Paulo, Brazil.
authors.forename: Glyn M
authors.initials: GM
authors.lastname: Figueira
authors.affiliation: Departamento de Genética, Universidade de São Paulo (USP), Av. Pádua Dias 11, 13418-900 Piracicaba, São Paulo, Brazil.
authors.forename: Miklos M
authors.initials: MM
authors.lastname: Bajay
authors.affiliation: Programa de Pós-graduação em Genética e Biologia Molecular, Universidade Estadual de Campinas, CP 6109, Rua Bertrand Russel, 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Jaqueline B
authors.initials: JB
authors.lastname: Campos
authors.affiliation: Programa de Pós-graduação em Genética e Biologia Molecular, Universidade Estadual de Campinas, CP 6109, Rua Bertrand Russel, 13083-970 Campinas, São Paulo, Brazil.
authors.forename: João P G
authors.initials: JP
authors.lastname: Viana
authors.affiliation: Departamento de Genética, Universidade de São Paulo (USP), Av. Pádua Dias 11, 13418-900 Piracicaba, São Paulo, Brazil.
authors.forename: José B
authors.initials: JB
authors.lastname: Pinheiro
authors.affiliation: Agência Paulista de Tecnologia dos Agronegócios, Pólo Centro Sul, CP 28, Rodovia SP 127, Km 30, 13400-970 Piracicaba, São Paulo, Brazil.
authors.forename: Maria I
authors.initials: MI
authors.lastname: Zucchi
keywords: Asteraceae; Baccharis dracunculifolia; altitude; microsatellites; population genetics