title: Host origin and tissue microhabitat shaping the microbiota of the terrestrial isopod Armadillidium vulgare.
abstract: We present the first in-depth investigation of the host-associated microbiota of the terrestrial isopod crustacean Armadillidium vulgare. This species is an important decomposer of organic matter in terrestrial ecosystems and a major model organism for arthropod-Wolbachia symbioses due to its well-characterized association with feminizing Wolbachia 16S rRNA gene pyrotags were used to characterize its bacterial microbiota at multiple levels: (i) in individuals from laboratory lineages and field populations and (ii) in various host tissues. This integrative approach allowed us to reveal an unexpectedly high bacterial diversity, placing this species in the same league as termites in terms of symbiotic diversity. Interestingly, both animal groups belong to the same ecological guild in terrestrial ecosystems. While Wolbachia represented the predominant taxon in infected individuals, it was not the only major player. Together, the most abundant taxa represented a large scope of symbiotic interactions, including bacterial pathogens, a reproductive parasite (Wolbachia) and potential nutritional symbionts. Furthermore, we demonstrate that individuals from different populations harboured distinct bacterial communities, indicating a strong link between the host-associated microbiota and environmental bacteria, possibly due to terrestrial isopod nutritional ecology. Overall, this work highlights the need for more studies of host-microbiota interactions and bacterial diversity in non-insect arthropods.
authors.affiliation: Université de Poitiers, UMR CNRS 7267, Ecologie et Biologie des Interactions, 5 rue Albert Turpain, TSA 51106, 86073 Poitiers, France dittmer@rowland.harvard.edu.
authors.forename: Jessica
authors.initials: J
authors.lastname: Dittmer
authors.affiliation: Université de Poitiers, UMR CNRS 7267, Ecologie et Biologie des Interactions, 5 rue Albert Turpain, TSA 51106, 86073 Poitiers, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Lesobre
authors.affiliation: Université de Poitiers, UMR CNRS 7267, Ecologie et Biologie des Interactions, 5 rue Albert Turpain, TSA 51106, 86073 Poitiers, France.
authors.forename: Bouziane
authors.initials: B
authors.lastname: Moumen
authors.affiliation: Université de Poitiers, UMR CNRS 7267, Ecologie et Biologie des Interactions, 5 rue Albert Turpain, TSA 51106, 86073 Poitiers, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Bouchon
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D033321:Isopoda / Q000382:microbiology* / Q000502:physiology; D064307:Microbiota; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013559:Symbiosis; D020577:Wolbachia / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Hepatoplasma; Wolbachia; bacterial diversity; host–symbiont interactions; microbiota; symbiosis