title: Co-expression of endoglucanase and β-glucosidase in Corynebacterium glutamicum DM1729 towards direct lysine fermentation from cellulose.
abstract: The aim of the present study is the development of a consolidated bioprocess for the production of lysine with recombinant Corynebacterium glutamicum DM1729 strains expressing endoglucanase and β-glucosidase genes. Here, the endoglucanase genes from Xanthomonas campestris XCC3521 and XCC2387 and betaglucosidase gene from Saccharophagus degradans Sde1394 were cloned in C. glutamicum DM1729 and expressed either extracellularly or on cell surface. The highest β-glucosidase activity of 9±0.5U/OD600 of 1 and endoglucanase activity of 5.5±0.8U was obtained in C. glutamicum DM 1729 (pVWEx1-TATXCC2387) (pEKEx3-PorC-Sde1394) when cellobiose (20g/L) alone or in combination with carboxymethyl cellulose (20g/L) was used as the carbon sources respectively. The overall efforts resulted in a lysine titre of 5.9±0.5mM. The ability of the constructs to utilize carboxymethyl cellulose and cellobiose for growth and amino acid production proves the concept of utilization of C. glutamicum as a biocatalyst in the lignocellulosic biorefinery.
authors.affiliation: Biotechnology Division, CSIR - National Institute For Interdisciplinary Science and Technology (NIIST), Trivandrum 695 019, Kerala, India.
authors.forename: Murali
authors.initials: M
authors.lastname: Anusree
authors.affiliation: Chair of Genetics of Prokaryotes, Bielefeld University, Universitaetsstr. 25, 33615 Bielefeld, Germany.
authors.forename: Volker F
authors.initials: VF
authors.lastname: Wendisch
authors.affiliation: Biotechnology Division, CSIR - National Institute For Interdisciplinary Science and Technology (NIIST), Trivandrum 695 019, Kerala, India. Electronic address: madhavan85@hotmail.com.
authors.forename: K Madhavan
authors.initials: KM
authors.lastname: Nampoothiri
mesh_terms: D001709:Biotechnology / Q000379:methods; D002266:Carboxymethylcellulose Sodium / Q000378:metabolism; D002475:Cellobiose / Q000378:metabolism; D002480:Cellulase / Q000235:genetics / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D048230:Corynebacterium glutamicum / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D008239:Lysine / Q000378:metabolism*; D012275:Oryza / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D031786:Saccharum / Q000378:metabolism; D016959:Xanthomonas campestris / Q000235:genetics; D001617:beta-Glucosidase / Q000235:genetics / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D002475:Cellobiose; D002482:Cellulose; D001617:beta-Glucosidase; D002480:Cellulase; D008239:Lysine; D002266:Carboxymethylcellulose Sodium
keywords: Biorefinery; Corynebacterium glutamicum; Endoglucanase; Lysine; β-Glucosidase