title: Pheno- and genotypic characterization of Pasteurella multocida isolated from cats, dogs and rabbits from Brazil.
abstract: Pasteurella multocida is the causative agent of many diseases of economic importance in veterinary medicine and is characterized by high zoonotic potential. Pet animals can be infected and play a major role as carriers. This study aimed to characterize the genetic diversity of P. multocida isolated from dogs, cats and rabbits, and to evaluate their antimicrobial susceptibility profiles. A total of 620 animals were studied; 51 were positive for P. multocida and 92 strains were isolated. 60.9% of the strains belonged to the capsular type A, while the remaining were classified as non-typeable. The hgbA, ptfA, sodC, tadD and hsf2 genes were more frequent among the rabbit strains. Sulfonamides and cotrimoxazole presented the highest resistance rate, followed by erythromycin. PFGE clustered strains according to host species. Our results indicate that P. multocida from companion animals carry several virulence factors and are resistant to antimicrobials commonly used in human and veterinary medicine.
authors.affiliation: Programa de Epidemiologia Experimental Aplicada às Zoonoses - Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Thais Sebastiana Porfida
authors.initials: TS
authors.lastname: Ferreira
authors.affiliation: Programa de Epidemiologia Experimental Aplicada às Zoonoses - Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Luisa Zanolli
authors.initials: LZ
authors.lastname: Moreno
authors.affiliation: Programa de Epidemiologia Experimental Aplicada às Zoonoses - Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Maria Roberta
authors.initials: MR
authors.lastname: Felizardo
authors.affiliation: Programa de Epidemiologia Experimental Aplicada às Zoonoses - Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Débora Dirani Sena
authors.initials: DD
authors.lastname: de Gobbi
authors.affiliation: Programa de Epidemiologia Experimental Aplicada às Zoonoses - Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Pedro Henrique de Lima Nogueira
authors.initials: PH
authors.lastname: Filsner
authors.affiliation: Programa de Epidemiologia Experimental Aplicada às Zoonoses - Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Vasco Tulio
authors.initials: VT
authors.lastname: de Moura Gomes
authors.affiliation: Programa de Epidemiologia Experimental Aplicada às Zoonoses - Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Marina
authors.initials: M
authors.lastname: Moreno
authors.affiliation: Programa de Epidemiologia Experimental Aplicada às Zoonoses - Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil. Electronic address: morenoam@usp.br.
authors.forename: Andrea Micke
authors.initials: AM
authors.lastname: Moreno
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001938:Brazil / Q000453:epidemiology; D002371:Cat Diseases / Q000382:microbiology; D002415:Cats / Q000382:microbiology*; D004283:Dog Diseases / Q000382:microbiology; D004285:Dogs / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D009643:Norfloxacin / Q000494:pharmacology; D010326:Pasteurella Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D016979:Pasteurella multocida / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011817:Rabbits / Q000382:microbiology*; D037521:Virulence Factors / Q000235:genetics; D015047:Zoonoses
chemical_list: D000900:Anti-Bacterial Agents; D037521:Virulence Factors; D009643:Norfloxacin
keywords: PFGE; Pasteurella multocida; Resistance; Virulence genes