title: Functional analysis of PI-like gene in relation to flower development from bamboo (Bambusa oldhamii).
abstract: Bamboo flowering owns many unique characteristics and remains a mystery. To investigate the molecular mechanisms underlying flower development in bamboo, a petal-identity gene was identified as a PISTILLATA homologue named BoPI from Bambusa oldhamii (bamboo family). Expression analysis showed that BoPI was highly expressed in flower organs and gradually increased during flower development stage, suggesting that BoPI played an important role in flower development. Ectopic expression of BoPI in Arabidopsis caused conversion of sepals to petals. 35S::BoPI fully rescued the defective petal formation in the pi-1 mutant. BoPI could interact with BoAP3 protein in vitro. These results suggested that BoPI regulated flower development of bamboo in a similar way with PI. Besides flower organs, BoPI was also expressed in leaf and branch, which revealed that BoPI may involve in leaf and branch development. Similar to other MIKC-type gene, BoPI contained the Cterminal sequence but its function was controversial. Ectopic expression of the C-terminal deletion construct (BoPI- ∆C) in Arabidopsis converted sepals to petals; BoPI- ∆C interacted with BoAP3 on yeast two-hybrid assay, just like the full-length con struct. The result implied that the C-terminal sequence may not be absolutely required for organ identity function in the context of BoPI.
authors.affiliation: The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Linan, Hangzhou 311300, People's Republic of China;linxcx@163.com.
authors.forename: Longfei
authors.initials: L
authors.lastname: Zhu
authors.forename: Yan
authors.initials: Y
authors.lastname: Shi
authors.forename: Qiaolu
authors.initials: Q
authors.lastname: Zang
authors.forename: Quan
authors.initials: Q
authors.lastname: Shi
authors.forename: Shinan
authors.initials: S
authors.lastname: Liu
authors.forename: Yingwu
authors.initials: Y
authors.lastname: Xu
authors.forename: Xinchun
authors.initials: X
authors.lastname: Lin
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D031723:Bambusa / Q000235:genetics / Q000254:growth & development*; D035264:Flowers / Q000254:growth & development*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid