title: Clostridium perfringens: Comparative effects of heat and osmotic stress on non-enterotoxigenic and enterotoxigenic strains.
abstract: Clostridium perfringens isolates associated with food poisoning carries a chromosomal cpe gene, while non-foodborne human gastrointestinal disease isolates carry a plasmid cpe gene. The enterotoxigenic strains tested produced vegetative cells and spores with significantly higher resistance than non-enterotoxigenic strains. These results suggest that the vegetative cells and spores have a competitive advantage over non-enterotoxigenic strains. However, no explanation has been provided for the significant associations between chromosomal cpe genotypes with the high resistance, which could explain the strong relationship between chromosomal cpe isolates and C. perfringens type A food poisoning. Here, we analyse the action of physical and chemical agent on non-enterotoxigenic and enterotoxigenic regional strains. And this study tested the relationship between the sensitivities of spores and their levels SASPs (small acid soluble proteins) production in the same strains examined.
authors.affiliation: IBAM-CONICET and Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Mendoza, Argentina. Electronic address: abbonacinthia@gmail.com.
authors.forename: Cinthia Carolina
authors.initials: CC
authors.lastname: Abbona
authors.affiliation: Departamento de Química Biológica Facultad de Química Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina. Electronic address: pvstag@unsl.edu.ar.
authors.forename: Patricia Virginia
authors.initials: PV
authors.lastname: Stagnitta
mesh_terms: D000818:Animals; D002417:Cattle; D002876:Chromosomes, Bacterial / Q000737:chemistry*; D003016:Clostridium perfringens / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D004768:Enterotoxins / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial; D006358:Hot Temperature; D006801:Humans; D008460:Meat / Q000032:analysis*; D009997:Osmotic Pressure; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism*; D017365:Spices / Q000032:analysis / Q000382:microbiology; D013171:Spores, Bacterial / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity
chemical_list: D004768:Enterotoxins
keywords: Antibiotic resistance; C-cpe strains; Clostridium perfringens; Food safety; Heat resistance; Osmotic stress; SASPs