title: Colonic Mucosal Epigenome and Microbiome Development in Children and Adolescents.
abstract: Epigenetic and microbiome changes during pediatric development have been implicated as important elements in the developmental origins of inflammatory bowel diseases (IBDs) including Crohn's disease (CD) and ulcerative colitis (UC), which are linked to early onset colorectal cancer (CRC). Colonic mucosal samples from 22 control children between 3.5 and 17.5 years of age were studied by Infinium HumanMethylation450 BeadChips and, in 10 cases, by 454 pyrosequencing of the bacterial 16S rRNA gene. Intercalating age-specific DNA methylation and microbiome changes were identified, which may have significant translational relevance in the developmental origins of IBD and CRC.
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: R Alan
authors.initials: RA
authors.lastname: Harris
authors.affiliation: Section of Gastroenterology and Hepatology, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Shah
authors.affiliation: Department of Pathology & Immunology, Baylor College of Medicine, Houston, TX 77030, USA; Texas Children's Hospital, Houston, TX 77030, USA.
authors.forename: Emily B
authors.initials: EB
authors.lastname: Hollister
authors.affiliation: Department of Clinical Science, University of Bergen, 5020 Bergen, Norway; Department of Pediatrics, Haukeland University Hospital, 5021 Bergen, Norway.
authors.forename: Rune Rose
authors.initials: RR
authors.lastname: Tronstad
authors.affiliation: Department of Clinical Medicine, University of Bergen, 5020 Bergen, Norway.
authors.forename: Nils
authors.initials: N
authors.lastname: Hovdenak
authors.affiliation: Department of Pathology & Immunology, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Reka
authors.initials: R
authors.lastname: Szigeti
authors.affiliation: Department of Pathology & Immunology, Baylor College of Medicine, Houston, TX 77030, USA; Texas Children's Hospital, Houston, TX 77030, USA.
authors.forename: James
authors.initials: J
authors.lastname: Versalovic
authors.affiliation: Texas Children's Hospital, Houston, TX 77030, USA; Section of Pediatric Gastroenterology, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA; USDA/ARS Children's Nutrition Research Center, Houston, TX 77030, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Kellermayer
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D003093:Colitis, Ulcerative / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D018899:CpG Islands / Q000276:immunology; D003424:Crohn Disease / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D019175:DNA Methylation; D044127:Epigenesis, Genetic / Q000276:immunology*; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology* / Q000382:microbiology; D008297:Male; D064307:Microbiota / Q000235:genetics / Q000276:immunology*; D011506:Proteins / Q000235:genetics / Q000276:immunology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D000074083:Sodium-Hydrogen Exchanger 3; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics / Q000276:immunology
chemical_list: C568062:KHDC3L protein, human; D011506:Proteins; D012336:RNA, Ribosomal, 16S; D000074083:Sodium-Hydrogen Exchanger 3; D017923:Sodium-Hydrogen Exchangers