title: First report of Leptolegnia chapmanii (Peronosporomycetes: Saprolegniales) affecting mosquitoes in central Brazil.
abstract: Numerous isolates of an oomycete 'fungus', Leptolegnia chapmanii, are reported from Brazil for the first time. This aquatic pathogen was baited with Aedes aegypti sentinel larvae from stagnant, temporary bodies of water in selected locations under secondary tropical forest in and near the central Brazilian city of Goiânia and from more distant sites in the western and northern regions of the state of Goiás. Isolates were identified based on their morphological and developmental characters, comparative sequence data for the ITS and TEF loci, as well as their rapid activity against A. aegypti larvae. Taxonomic issues affecting the application of the name L. chapmanii and its typification are rectified. This study contributes to a better understanding of the presence and distribution of this oomycete in Brazil, its sequence-based identification, and of its potential as a biological agent against mosquito vectors.
authors.affiliation: Laboratório de Patologia de Invertebrados, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
authors.forename: Cristian
authors.initials: C
authors.lastname: Montalva
authors.affiliation: Laboratório de Patologia de Invertebrados, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
authors.forename: Karine
authors.initials: K
authors.lastname: Dos Santos
authors.affiliation: Centro Universitário de Gurupí (UnirG), Gurupí, Tocantins, Brazil.
authors.forename: Karin
authors.initials: K
authors.lastname: Collier
authors.affiliation: Laboratório de Patologia de Invertebrados, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, Goiás, Brazil; Instituto Federal de Educação, Ciência e Tecnologia de Goiás (IFG), Aparecida de Goiânia, Goiás, Brazil.
authors.forename: Luiz F N
authors.initials: LF
authors.lastname: Rocha
authors.affiliation: Laboratório de Patologia de Invertebrados, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
authors.forename: Éverton K K
authors.initials: ÉK
authors.lastname: Fernandes
authors.affiliation: Department of Entomology, Cornell University, Ithaca, NY, USA.
authors.forename: Louela A
authors.initials: LA
authors.lastname: Castrillo
authors.affiliation: Laboratório de Patologia de Invertebrados, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
authors.forename: Christian
authors.initials: C
authors.lastname: Luz
authors.affiliation: USDA-ARS Emerging Pests and Pathogens Research Unit, Robert W. Holley Center for Agriculture and Health, Ithaca, NY, USA. Electronic address: richard.humber@ars.usda.gov.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Humber
mesh_terms: D000330:Aedes / Q000382:microbiology*; D000818:Animals; D001938:Brazil; D044742:Peronospora / Q000145:classification*; D016133:Polymerase Chain Reaction
keywords: Aedes; Culicidae; Distribution; Epitype; Lectotype; Pathogen; Watermold