title: Phytochemicals from Camellia nitidissima Chi inhibited the formation of advanced glycation end-products by scavenging methylglyoxal.
abstract: The objective of this study was to investigate the inhibitory effects of Camellia nitidissima Chi (CNC) on the advanced glycation end-product (AGE) formation. CNC was extracted with ethanol and further separated into dichloromethane, ethyl acetate, n-butanol, and water soluble fractions. Ethyl acetate fraction had the highest total phenolic and quercetin content compared with other fractions. Sixteen phenolic compounds were identified using HPLC Triple TOF MS/MS. Bovine serum albumin (BSA)-glucose assay showed that dichloromethane and ethyl acetate fraction inhibited AGE formation by 88.1% and 87.5% at 2.5mg/mL. BSA-methylglyoxal assay showed that ethyl acetate fraction inhibited 54.1% AGE formation while dichloromethane fraction inhibited 28.1%. Over 96.0% of methylglyoxal was scavenged by different fractions within 12h. Both mono- and di-methylglyoxal quercetin adducts were identified after incubating quercetin with methylglyoxal using HPLC-ESI-MS(n). The results in this study suggest that CNC extracts inhibited AGEs formation in part through scavenging methylglyoxal by phenolic compounds.
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China; Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA.
authors.forename: Weixin
authors.initials: W
authors.lastname: Wang
authors.affiliation: Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Liu
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Zhennan
authors.initials: Z
authors.lastname: Wang
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Qi
authors.affiliation: Jiangsu Center for Pharmacodynamics Research and Evaluation, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Shengtao
authors.initials: S
authors.lastname: Yuan
authors.affiliation: Guangxi Gui RenTang Co., Ltd, Fangchenggang 538021, China.
authors.forename: Weijie
authors.initials: W
authors.lastname: Zhang
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Chen
authors.affiliation: School of Nutrition and Food Science, Louisiana State University, Baton Rouge, LA 34202, USA.
authors.forename: John W
authors.initials: JW
authors.lastname: Finley
authors.affiliation: Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA.
authors.forename: Liwei
authors.initials: L
authors.lastname: Gu
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. Electronic address: aiqunj302@njust.edu.cn.
authors.forename: Ai-Qun
authors.initials: AQ
authors.lastname: Jia
mesh_terms: D028244:Camellia / Q000737:chemistry*; D017127:Glycation End Products, Advanced / Q000737:chemistry*; D064209:Phytochemicals / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D011765:Pyruvaldehyde / Q000737:chemistry*; D011794:Quercetin / Q000737:chemistry*
chemical_list: D017127:Glycation End Products, Advanced; D064209:Phytochemicals; D010936:Plant Extracts; D011765:Pyruvaldehyde; D011794:Quercetin
keywords: Advanced glycation end-products; Camellia nitidissima Chi; Quercetin; Scavenging methylglyoxal