title: Pigment Epithelium-Derived Factor Inhibits Oxygen-Induced Retinal Neovascularization in a Murine Model.
abstract: BACKGROUND
  The inhibitory effects of pigment epithelium-derived factor (PEDF) on retinal neovascularization were observed in a murine model of oxygen-induced retinopathy.
  
  
  METHODS
  PEDF was administered via intravitreal injection into the right eye of mice subjected to hyperoxia. The proliferative neovascular response was assessed by evaluating the vascular pattern in retinal flat-mounts and quantified by counting the number of new vascular cell nuclei extending into the internal limiting membrane. Retinas were stained for vascular endothelial growth factor (VEGF), PEDF, and CD31 with immunohistochemical method.
  
  
  RESULTS
  The effect of PEDF on retinal neovascularization is related to the postnatal age at the time of injection and the number of injections. The injection of 2 µg of PEDF at postnatal day 12 (P12) and P14 markedly inhibited retinal neovascularization.
  
  
  CONCLUSIONS
  Locally administered PEDF is a potentially effective vascular inhibitory factor for the treatment and prevention of retinopathy of prematurity.
authors.affiliation: a Department of Neonatology , Children's Hospital of Fudan University , Shanghai , China.
authors.forename: Shulian
authors.initials: S
authors.lastname: Zhang
authors.affiliation: b Department of Pediatrics , Minhang Hospital of Fudan University , Shanghai , China.
authors.forename: Guanpeng
authors.initials: G
authors.lastname: Zhai
authors.affiliation: a Department of Neonatology , Children's Hospital of Fudan University , Shanghai , China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Shi
authors.affiliation: a Department of Neonatology , Children's Hospital of Fudan University , Shanghai , China.
authors.forename: Yuhuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: a Department of Neonatology , Children's Hospital of Fudan University , Shanghai , China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.affiliation: a Department of Neonatology , Children's Hospital of Fudan University , Shanghai , China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Dai
authors.affiliation: a Department of Neonatology , Children's Hospital of Fudan University , Shanghai , China.
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005136:Eye Proteins / Q000378:metabolism*; D018496:Hyperoxia / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D008810:Mice, Inbred C57BL; D009414:Nerve Growth Factors / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D015861:Retinal Neovascularization / Q000378:metabolism* / Q000473:pathology*; D015843:Serpins / Q000378:metabolism*; D000099174:Pigment Epithelium-Derived Factor
chemical_list: D005136:Eye Proteins; D009414:Nerve Growth Factors; D010100:Oxygen; D015843:Serpins; D000099174:Pigment Epithelium-Derived Factor
keywords: pigment epithelium-derived factor; retinal neovascularization; retinopathy of prematurity