title: Salmonella Typhimurium infection primes a nutriprive mechanism in piglets.
abstract: Salmonella enterica serovar Typhimurium (S. Typhimurium) is an important cause of acute food- borne zoonoses worldwide, typically carried by pigs. It is well known that Salmonella has evolved a wide array of strategies enabling it to invade the host, but little information is available on the specific host responses to Salmonella infections. In the present study, we used an in vivo approach (involving piglets infected with a virulent or an attenuated S. Typhimurium strain) coupled to histological and proteomic analysis of the cecum mucosa, to highlight the host pathways activated during S. Typhimurium infection. We confirm the complex host-pathogen interaction. Our data showed that the metabolic and the cytoskeleton organization functions were the most significantly altered. In particular, the modifications of energy metabolic pathway could suggest a "nutriprive" mechanism, in which the host reduce its metabolic and energetic status to limit Salmonella infection. This study could represent a preliminary approach, providing information useful to better understand the host-Salmonella interaction.
authors.affiliation: Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Monterotondo (RM), Italy. Electronic address: maria.miarelli@crea.gov.it.
authors.forename: Maria
authors.initials: M
authors.lastname: Miarelli
authors.affiliation: Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, Rome, Italy; Department of Comparative Biomedicine and Food Science, University of Padova, Agripolis, Legnaro (PD), Italy.
authors.forename: Rosanna
authors.initials: R
authors.lastname: Drumo
authors.affiliation: Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Monterotondo (RM), Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Signorelli
authors.affiliation: Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Monterotondo (RM), Italy.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Marchitelli
authors.affiliation: Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Perugia, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Pavone
authors.affiliation: Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, Rome, Italy; VISAVET Health Surveillance Centre, Universidad Complutense Madrid, Madrid, Spain.
authors.forename: Michele
authors.initials: M
authors.lastname: Pesciaroli
authors.affiliation: Department of Veterinary Diagnostic, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Brescia, Italy.
authors.forename: Jessica
authors.initials: J
authors.lastname: Ruggieri
authors.affiliation: Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Chirullo
authors.affiliation: Department of Biology, University of Roma Tor Vergata, Rome, Italy.
authors.forename: Serena
authors.initials: S
authors.lastname: Ammendola
authors.affiliation: Department of Biology, University of Roma Tor Vergata, Rome, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Battistoni
authors.affiliation: Department of Veterinary Diagnostic, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Brescia, Italy.
authors.forename: Giovanni L
authors.initials: GL
authors.lastname: Alborali
authors.affiliation: Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Perugia, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Manuali
authors.affiliation: Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Pasquali
mesh_terms: D000818:Animals; D002432:Cecum / Q000382:microbiology / Q000503:physiopathology; D003599:Cytoskeleton / Q000473:pathology; D005786:Gene Expression Regulation / Q000276:immunology; D054884:Host-Pathogen Interactions / Q000276:immunology*; D007422:Intestines / Q000276:immunology / Q000382:microbiology; D020543:Proteome; D012481:Salmonella Infections, Animal / Q000276:immunology*; D012486:Salmonella typhimurium / Q000276:immunology / Q000472:pathogenicity; D013552:Swine; D013553:Swine Diseases / Q000276:immunology
chemical_list: D020543:Proteome
keywords: Attenuated strain; Histological analysis; Piglets; Proteoma; Salmonella Typhimurium; Virulent strain