title: A novel integrative approach to identify lncRNAs associated with the survival of melanoma patients.
abstract: Long non-coding RNAs (lncRNAs) play important roles in diagnosis and prognosis of human cancers. With the development of microarray and RNA-seq, gene expression were measured in more and more tumor types for identification of prognostic markers. However, lncRNA expression profiles of tumor patients with follow-up information were rare. In this study, we developed a novel simple computational approach, which didn't use lncRNA expression, to identify lncRNAs associated with the survival of melanoma patients through integrating gene expression and lncRNA-target networks. First, we calculated the significance of associations between gene expression and patients' survival. Second, we constructed the experimentally validated lncRNA-target gene networks. Next, the significance of lncRNAs were obtained by combination of the p-values of their neighbor genes. Finally, we identified 15 lncRNAs that were significantly associated with the survival of melanoma patients (p<0.05), which were supported by functional analysis and literature review. Collectively, this study provides an effective approach to predict the lncRNA signatures for outcomes of tumor patients without lncRNA expression profiles.
authors.affiliation: Department of Anatomy, Qiqihar Medical College, Qiqihar 161006, China. Electronic address: linnaguo@tom.com.
authors.forename: Linna
authors.initials: L
authors.lastname: Guo
authors.affiliation: Department of Anatomy, Qiqihar Medical College, Qiqihar 161006, China.
authors.forename: Lijie
authors.initials: L
authors.lastname: Yao
authors.affiliation: Department of Anatomy, Qiqihar Medical College, Qiqihar 161006, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D020869:Gene Expression Profiling; D006801:Humans; D008545:Melanoma / Q000235:genetics* / Q000503:physiopathology*; D062085:RNA, Long Noncoding / Q000235:genetics*; D016019:Survival Analysis
chemical_list: D062085:RNA, Long Noncoding
keywords: Melanoma; Prognosis; Survival analysis; TCGA; lncRNA