title: Two new polyhydroxysterols produced by Fusarium solani, an endophytic fungus from Chloranthus multistachys.
abstract: A highly antagonistic endophytic fungus, designated strain CL39, was originated from the leaves of Chloranthus multistachys collected in Wulong of Chongqing municipality of China in November 2015. The strain was identified as Fusarium solani based on morphological characteristics, 5.8S gene and internal transcribed spacer sequence analysis. Two new compounds, 2β, 9α-dihydroxy-5α-methoxyergosta-7, 22-diene (1), 2β, 6β-dihydroxy-5α-methoxyergosta-7, 22-diene (2) have been isolated from the culture broth of the strain. Structures of the new compounds were elucidated by detailed analysis of their spectroscopic data aided by the comparison with reported data of related derivatives, and found to belong to the polyhydroxylated steroids with a hydroxyl at C-2 instead of C-3, a rare structure among the steroids. The extract of this strain and all isolated compounds were evaluated for their antagonistic activities.
authors.affiliation: a Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Life Science , Southwest University , Chongqing , People's Republic of China.|b First Affiliated Hospital, Huzhou Teachers College , The First People's Hospital of Huzhou , Huzhou , China.
authors.forename: W Y
authors.initials: WY
authors.lastname: Shen
authors.affiliation: a Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Life Science , Southwest University , Chongqing , People's Republic of China.
authors.forename: R
authors.initials: R
authors.lastname: Bai
authors.affiliation: c Feed Research Institute, Chinese Academy of Agricultural Sciences , Beijing , People's Republic of China.
authors.forename: A R
authors.initials: AR
authors.lastname: Wang
authors.affiliation: a Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Life Science , Southwest University , Chongqing , People's Republic of China.
authors.forename: J Y
authors.initials: JY
authors.lastname: He
authors.affiliation: a Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Life Science , Southwest University , Chongqing , People's Republic of China.
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.affiliation: a Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Life Science , Southwest University , Chongqing , People's Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: a Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Life Science , Southwest University , Chongqing , People's Republic of China.
authors.forename: X F
authors.initials: XF
authors.lastname: Zhao
authors.affiliation: a Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Life Science , Southwest University , Chongqing , People's Republic of China.
authors.forename: J Y
authors.initials: JY
authors.lastname: Dong
mesh_terms: D000890:Anti-Infective Agents / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D002681:China; D004353:Drug Evaluation, Preclinical / Q000379:methods; D060026:Endophytes / Q000737:chemistry / Q000378:metabolism; D005670:Fusarium / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D019684:Magnoliopsida / Q000382:microbiology; D015394:Molecular Structure; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000382:microbiology; D013261:Sterols / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D013261:Sterols
keywords: Chloranthus multistachys; Fusarium; antagonistic activities; endophytic fungus; polyhydroxysterols