title: Rapid and sensitive monitoring of biocatalytic reactions using ion mobility mass spectrometry.
abstract: The combination of stable isotope labelling with direct infusion ion mobility mass spectrometry (IM-MS) enabled qualitative and quantitative monitoring of biocatalytic reactions with reduced analysis times, enhanced sensitivity and μL-level assay volumes. The new approach was demonstrated by applying to both lipase and monooxygenase enzymes, including multi-substrate screening.
authors.affiliation: School of Chemistry, Manchester Institute of Biotechnology, The University of Manchester, 131 Princess Street, Manchester M1 7DN, UK. perdita.barran@manchester.ac.uk sabine.flitsch@manchester.ac.uk.
authors.forename: Cunyu
authors.initials: C
authors.lastname: Yan
authors.forename: Jason W
authors.initials: JW
authors.lastname: Schmidberger
authors.forename: Fabio
authors.initials: F
authors.lastname: Parmeggiani
authors.forename: Shaneela A
authors.initials: SA
authors.lastname: Hussain
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Turner
authors.forename: Sabine L
authors.initials: SL
authors.lastname: Flitsch
authors.forename: Perdita
authors.initials: P
authors.lastname: Barran
mesh_terms: D000588:Amines / Q000737:chemistry; D055162:Biocatalysis; D004952:Esters; D008049:Lipase / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D044223:Pseudomonas stutzeri / Q000201:enzymology; D013997:Time Factors
chemical_list: D000588:Amines; D004952:Esters; D008049:Lipase