title: Soy Isoflavone Protects Myocardial Ischemia/Reperfusion Injury through Increasing Endothelial Nitric Oxide Synthase and Decreasing Oxidative Stress in Ovariectomized Rats.
abstract: There is a special role for estrogens in preventing and curing cardiovascular disease in women. Soy isoflavone (SI), a soy-derived phytoestrogen, has similar chemical structure to endogenous estrogen-estradiol. We investigate to elucidate the protective mechanism of SI on myocardial ischemia/reperfusion (MI/R) injury. Female SD rats underwent bilateral ovariectomy. One week later, rats were randomly divided into several groups, sham ovariectomy (control group), ovariectomy with MI/R, or ovariectomy with sham MI/R. Other ovariectomy rats were given different doses of SI or 17β-estradiol (E2). Four weeks later, they were exposed to 30 minutes of left coronary artery occlusion followed by 6 or 24 hours of reperfusion. SI administration significantly reduced myocardial infarct size and improved left ventricle function and restored endothelium-dependent relaxation function of thoracic aortas after MI/R in ovariectomized rats. SI also decreased serum creatine kinase and lactate dehydrogenase activity, reduced plasma malonaldehyde, and attenuated oxidative stress in the myocardium. Meanwhile, SI increased phosphatidylinositol 3 kinase (PI3K)/Akt/endothelial nitric oxide synthase (eNOS) signal pathway. SI failed to decrease infarct size of hearts with I/R in ovariectomized rats if PI3K was inhibited. Overall, these results indicated that SI protects myocardial ischemia/reperfusion injury in ovariectomized rats through increasing PI3K/Akt/eNOS signal pathway and decreasing oxidative stress.
authors.affiliation: Key Laboratory of Cardiovascular Disease and Molecular Intervention, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Key Laboratory of Cardiovascular Disease and Molecular Intervention, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Shuangyue
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Gynaecology, The First Public Hospital of Zhangjiagang, Zhangjiagang 215699, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Aoyang Hospital, Zhangjiagang 215600, China.
authors.forename: Jinbiao
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Cardiovascular Disease and Molecular Intervention, Nanjing Medical University, Nanjing 210029, China; Department of Pharmacology, School of Pharmacy, Nantong University, Nantong 226001, China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Meng
authors.affiliation: Key Laboratory of Cardiovascular Disease and Molecular Intervention, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Xie
authors.affiliation: Key Laboratory of Food Safety Research, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Key Laboratory of Cardiovascular Disease and Molecular Intervention, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Ji
authors.affiliation: Department of Geriatrics, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Han
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000473:pathology; D001835:Body Weight / Q000187:drug effects; D003402:Creatine Kinase / Q000097:blood; D004958:Estradiol / Q000097:blood; D005260:Female; D006334:Heart Function Tests / Q000187:drug effects; D007529:Isoflavones / Q000494:pharmacology / Q000627:therapeutic use*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D009203:Myocardial Infarction / Q000150:complications / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000201:enzymology* / Q000473:pathology / Q000503:physiopathology; D009206:Myocardium / Q000473:pathology; D000072000:NF-KappaB Inhibitor alpha / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D009929:Organ Size / Q000187:drug effects; D010052:Ovariectomy; D018384:Oxidative Stress / Q000187:drug effects; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D013025:Glycine max / Q000737:chemistry*; D015854:Up-Regulation / Q000187:drug effects; D014599:Uterus / Q000187:drug effects / Q000473:pathology; D014664:Vasodilation / Q000187:drug effects
chemical_list: D007529:Isoflavones; D000072000:NF-KappaB Inhibitor alpha; D004958:Estradiol; D007770:L-Lactate Dehydrogenase; D052250:Nitric Oxide Synthase Type III; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D003402:Creatine Kinase