title: Relationships between plasma leptin levels, leptin G2548A, leptin receptor Gln223Arg polymorphisms and gestational diabetes mellitus in Chinese population.
abstract: The purposes of this study were to examine concentrations of leptin and biochemical parameters in gestational diabetes mellitus (GDM) patients and normal glucose tolerance (NGT) individuals, and also to explore the links of leptin (LEP) G2548A and leptin receptor (LEPR) Gln223Arg polymorphisms with leptin levels and GDM risk among Chinese. Our study included 357 GDM and 355 NGT individuals who were at 24~30 gestational weeks. Plasma leptin and insulin levels were analyzed by ELISA. Gene polymorphisms were genotyped using TaqMan real-time polymerase chain reaction assay. The results showed that plasma leptin levels were significantly higher in the impaired fasting glucose (IFG) group than NGT group (34.35 (26.54, 56.48) ng/mL vs 26.31 (17.99, 37.87) ng/mL, P < 0.05). Plasma leptin levels correlated with plasma fasting insulin levels, pre-pregnant body mass index, homeostasis model assessment-insulin resistance and quantitative insulin sensitivity check index both in GDM and NGT group (P < 0.05). However, neither LEP G2548A nor LEPR Gln223Arg polymorphisms were significantly associated with GDM risk and plasma leptin levels (P > 0.05). Our findings showed that high leptin level was associated with GDM. And larger and more rigorous researches were needed to further explore the association of LEP and LEPR gene polymorphisms and GDM among Chinese population.
authors.affiliation: Department of Maternal and Child Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Maternal and Child Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Songxu
authors.initials: S
authors.lastname: Peng
authors.affiliation: Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Maternal and Child Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Wan
authors.affiliation: Department of Maternal and Child Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Fan
authors.affiliation: Department of Maternal and Child Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Yukai
authors.initials: Y
authors.lastname: Du
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics; D001786:Blood Glucose / Q000032:analysis; D016640:Diabetes, Gestational / Q000097:blood / Q000208:ethnology / Q000235:genetics*; D005215:Fasting / Q000097:blood; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005951:Glucose Tolerance Test; D006801:Humans; D007328:Insulin / Q000097:blood; D020738:Leptin / Q000097:blood / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D060888:Real-Time Polymerase Chain Reaction; D054411:Receptors, Leptin / Q000235:genetics*; D055815:Young Adult
chemical_list: D001786:Blood Glucose; D007328:Insulin; C515448:LEPR protein, human; D020738:Leptin; D054411:Receptors, Leptin