title: Natural Antibodies to Tumor-Associated Antigens.
abstract: Natural autoantibodies raised by humoral immune response to cancer can be exploited to identify potential tumor-associated antigens (TAAs), and might constitute new putative prognostic and/or diagnostic biomarkers. Here we describe how sera from tumor patients can be used to identify TAAs by screening antibody immunoreactivity against the cancer proteome resolved by two-dimensional gel electrophoresis.
authors.affiliation: Proteome Biochemistry Unit, IRCCS San Raffaele Hospital, Via Olgettina 58, 21032, Milano, Italy.
authors.forename: Sheila María
authors.initials: SM
authors.lastname: Álvarez-Fernández
authors.affiliation: Tumor Immunology Unit, IRCCS San Raffaele Hospital, Milano, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: De Monte
authors.affiliation: Proteome Biochemistry Unit, IRCCS San Raffaele Hospital, Via Olgettina 58, 21032, Milano, Italy. m.alessio@hsr.it.
authors.forename: Massimo
authors.initials: M
authors.lastname: Alessio
mesh_terms: D000912:Antibodies, Neoplasm / Q000097:blood / Q000302:isolation & purification*; D000951:Antigens, Neoplasm / Q000097:blood / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood / Q000302:isolation & purification; D045744:Cell Line, Tumor; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D020543:Proteome / Q000378:metabolism
chemical_list: D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D001323:Autoantibodies; D020543:Proteome
keywords: 2D-electrophoresis; Autoantibodies; Immunoproteomics; Tumor-associated antigens; Western blot