title: Catch and Release DNA Decoys: Capture and Photochemical Dissociation of NF-κB Transcription Factors.
abstract: Catch and release DNA decoys (CRDDs) are a new class of non-natural DNA probes that capture and dissociate from DNA-binding proteins using a light trigger. Photolytic cleavage of non-natural nucleobases in the CRDD yields abasic sites and truncation products that lower the affinity of the CRDD for its protein target. Herein, we demonstrate the ability of the first-generation CRDD to bind and release NF-κB proteins. This platform technology should be applicable to other DNA-binding proteins by modification of the target sequence.
authors.affiliation: Department of Medicinal Chemistry, University of Minnesota , 2231 6th Street S.E., Minneapolis, Minnesota 55455, United States.
authors.forename: Nicholas B
authors.initials: NB
authors.lastname: Struntz
authors.affiliation: Department of Medicinal Chemistry, University of Minnesota , 2231 6th Street S.E., Minneapolis, Minnesota 55455, United States.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Harki
mesh_terms: D015342:DNA Probes / Q000737:chemistry*; D003854:Deoxyribonucleotides / Q000737:chemistry*; D024202:Electrophoretic Mobility Shift Assay; D007211:Indoles / Q000737:chemistry*; D016328:NF-kappa B / Q000737:chemistry*; D011485:Protein Binding; D014466:Ultraviolet Rays
chemical_list: D015342:DNA Probes; D003854:Deoxyribonucleotides; D007211:Indoles; D016328:NF-kappa B