title: [GH10 Family of Glycoside Hydrolases: Structure and Evolutionary Connections].
abstract: Evolutionary connections were analyzed for endo-β-xylanases, which possess the GH10 family catalytic domains. A homology search yielded thrice as many proteins as are available from the Carbohydrate-Active Enzymes (CAZy) database. Lateral gene transfer was shown to play an important role in evolution of bacterial proteins of the family, especially in the phyla Acidobacteria, Cyanobacteria, Planctomycetes, Spirochaetes, and Verrucomicrobia. In the case of Verrucomicrobia, 23 lateral transfers from organisms of other phyla were detected. Evolutionary relationships were observed between the GH10 family domains and domains with the TIM-barrel tertiary structure from several other glycosidase families. The GH39 family of glycoside hydrolases showed the closest relationship. Unclassified homologs were grouped into 12 novel families of putative glycoside hydrolases (GHL51-GHL62).
authors.affiliation: Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, 117312 Russia.|daniil_naumoff@yahoo.com.
authors.forename: D G
authors.initials: DG
authors.lastname: Naumoff
mesh_terms: D001419:Bacteria / Q000201:enzymology* / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics; D020134:Catalytic Domain / Q000235:genetics; D019143:Evolution, Molecular; D022761:Gene Transfer, Horizontal; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000145:classification* / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D006026:Glycoside Hydrolases
keywords: GH-A clan; GH10 family; GHL families; PF00331 family; PSI Protein Classifier; PSI-BLAST; Verrucomicrobia; carbohydrate metabolism; endoglycosidase; gene annotation; glycoside hydrolase; lateral transfer; new protein families; protein evolution; protein superfamily; search for homologs; xylan utilization; β-xylanase