title: Isolation and Expansion of Muscle Precursor Cells from Human Skeletal Muscle Biopsies.
abstract: One of the major issues concerning human skeletal muscle progenitor cells is represented by the efficient isolation and in vitro expansion of cells retaining the ability to proliferate, migrate and differentiate once transplanted. Here we describe a method (1) effective in obtaining human muscle precursor cells both from fresh and frozen biopsies coming from different muscles, (2) selective to yield cells uniformly positive for CD56 and negative for CD34 without FACS sorting, (3) reliable in maintaining proliferative and in vitro differentiative capacity up to passage 10.
authors.affiliation: Stem cells and Regenerative Medicine Laboratory, Fondazione Istituto di Ricerca Pediatrica Città della Speranza, Corso Stati Uniti 4, 35127, Padova, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Franzin
authors.affiliation: Stem cells and Regenerative Medicine Laboratory, Fondazione Istituto di Ricerca Pediatrica Città della Speranza, Corso Stati Uniti 4, 35127, Padova, Italy.
authors.forename: Martina
authors.initials: M
authors.lastname: Piccoli
authors.affiliation: Stem Cells and Regenerative Medicine Section, Developmental Biology and Cancer Programme, UCL Institute of Child Health and Great Ormond Street Hospital, London, UK.
authors.forename: Luca
authors.initials: L
authors.lastname: Urbani
authors.affiliation: Department of Surgery, Oncology and Gastroenterology DiSCOG, Orthopedic Clinic, University of Padova, Padova, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Biz
authors.affiliation: Pediatric Surgery Unit, Department of Women's and Children's Health, University of Padova, Padova, Italy.
authors.forename: Piergiorgio
authors.initials: P
authors.lastname: Gamba
authors.affiliation: Stem Cells and Regenerative Medicine Section, Developmental Biology and Cancer Programme, UCL Institute of Child Health and Great Ormond Street Hospital, London, UK.
authors.forename: Paolo
authors.initials: P
authors.lastname: De Coppi
authors.affiliation: Stem cells and Regenerative Medicine Laboratory, Fondazione Istituto di Ricerca Pediatrica Città della Speranza, Corso Stati Uniti 4, 35127, Padova, Italy. m.pozzobon@irpcds.org.
authors.forename: Michela
authors.initials: M
authors.lastname: Pozzobon
mesh_terms: D001706:Biopsy; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D019070:Cell Lineage / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D002469:Cell Separation / Q000379:methods*; D006801:Humans; D018482:Muscle, Skeletal / Q000166:cytology*; D032446:Myoblasts / Q000166:cytology*
keywords: CD56; Enzymatic digestion; Human muscle cell primary culture; Human muscle precursor cells; Skeletal muscle biopsy