title: Association study for the role of Matrix metalloproteinases 2 and 3 gene polymorphisms in dental caries susceptibility.
abstract: OBJECTIVE
  Various exogenous and endogenous risk factors have been described as contributing to dental caries susceptibility. In the last decade it has been established that both pro and active forms of host derived Matrix metalloproteinases (MMPs) are present in the oral cavity. MMPs role in caries development has been hypothesized. The aim of this study was to analyse MMP2 (rs2287074) and MMP3 (rs679620) single nucleotide polymorphisms (SNPs) and their role in caries susceptibility.
  
  
  METHODS
  The two SNPs were analysed by PCR- restriction fragment length polymorphism (RFLP) in a sample of 102 ethnic Bulgarian volunteers (42 males and 60 females), all students in Sofia Medical University.
  
  
  RESULTS
  Statistical analysis of the MMP2 SNP showed significant differences for the genotype frequencies between the caries free (CF, DMFT=0) and low caries experience (LCE, DMFT≤5) groups. Analysis for the non-synonymous MMP3 SNP found significant differences between both CF vs caries experience groups (LCE+ high caries experience (HCE, DMFT≥5)) and LCE vs HCE groups. The presence of allele G decreased the risk of HCE about 4 times.
  
  
  CONCLUSIONS
  MMP2 and MMP3 genes are likely to be involved in caries susceptibility in our population. However, as dental caries is a multifactorial disorder and several genes are likely to have influence on it, it is reasonable to expect that SNPs, even those proven to be functional like rs679620, potentially play a significant, but not major role in the disease outcome.
authors.affiliation: Department of Conservative Dentistry, Faculty of Dental Medicine, Sofia Medical University, 1 Sv. G. Sofiiski Blvd., Sofia 1431, Bulgaria.
authors.forename: Dobrina
authors.initials: D
authors.lastname: Karayasheva
authors.affiliation: Genetic Medico-Diagnostic Laboratory Genica, 90 Tsar Asen str., Sofia 1643, Bulgaria.
authors.forename: Maria
authors.initials: M
authors.lastname: Glushkova
authors.affiliation: Department of Conservative Dentistry, Faculty of Dental Medicine, Sofia Medical University, 1 Sv. G. Sofiiski Blvd., Sofia 1431, Bulgaria.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Boteva
authors.affiliation: Department of Medical Chemistry and Biochemistry, Medical Faculty, Sofia Medical University, 2 Zdrave str., Sofia 1431, Bulgaria.
authors.forename: Vanyo
authors.initials: V
authors.lastname: Mitev
authors.affiliation: Genetic Medico-Diagnostic Laboratory Genica, 90 Tsar Asen str., Sofia 1643, Bulgaria; Department of Medical Chemistry and Biochemistry, Medical Faculty, Sofia Medical University, 2 Zdrave str., Sofia 1431, Bulgaria. Electronic address: kadiyska_t@yahoo.com.
authors.forename: Tanya
authors.initials: T
authors.lastname: Kadiyska
mesh_terms: D000328:Adult; D000483:Alleles; D003731:Dental Caries / Q000201:enzymology* / Q000235:genetics*; D003733:Dental Caries Susceptibility / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics*; D019278:Matrix Metalloproteinase 3 / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
chemical_list: C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2
keywords: Candidate genes; Dental caries; MMP2; MMP3; Matrix metalloproteinases