title: Transcribing RNA polymerase III observed by electron cryomicroscopy.
abstract: Electron cryomicroscopy reconstructions of elongating RNA polymerase (Pol) III at 3.9 Å resolution and of unbound Pol III (apo Pol III) in two distinct conformations at 4.6 Å and 4.7 Å resolution allow the construction of complete atomic models of Pol III and provide new functional insights into the adaption of Pol III to fulfill its specific transcription tasks.
authors.affiliation: European Molecular Biology Laboratory (EMBL), Structural and Computational Biology Unit, Heidelberg, Germany.
authors.forename: Niklas A
authors.initials: NA
authors.lastname: Hoffmann
authors.affiliation: European Molecular Biology Laboratory (EMBL), Structural and Computational Biology Unit, Heidelberg, Germany.
authors.forename: Arjen J
authors.initials: AJ
authors.lastname: Jakobi
authors.affiliation: European Molecular Biology Laboratory (EMBL), Structural and Computational Biology Unit, Heidelberg, Germany.
authors.forename: Matthias K
authors.initials: MK
authors.lastname: Vorländer
authors.affiliation: European Molecular Biology Laboratory (EMBL), Structural and Computational Biology Unit, Heidelberg, Germany.
authors.forename: Carsten
authors.initials: C
authors.lastname: Sachse
authors.affiliation: European Molecular Biology Laboratory (EMBL), Structural and Computational Biology Unit, Heidelberg, Germany.
authors.forename: Christoph W
authors.identifier: 0000-0003-2176-8337
authors.initials: CW
authors.lastname: Müller
mesh_terms: D020285:Cryoelectron Microscopy; D006801:Humans; D008958:Models, Molecular; D012320:RNA Polymerase III / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D014158:Transcription, Genetic
chemical_list: D012320:RNA Polymerase III
keywords: Pol III; RNA polymerase III; electron cryomicroscopy; tRNA; transcription