title: DENGUE OUTBREAK IN MATO GROSSO STATE, MIDWESTERN BRAZIL.
abstract: Dengue virus (DENV) is the most frequent arbovirus worldwide. In this study, we report a large outbreak in Mato Grosso State (MT). Serum samples from 604 patients with acute febrile illness for less than five days were inoculated in C6/36 cells, then infected cells were subjected to an indirect immunofluorescence test for DENV serotypes and yellow fever virus. Serum samples were submitted to a multiplex-semi-nested-RT-PCR for 11 flaviviruses. DENV-4 was isolated in 150/604 (24.8%) and DENV-1 in 19/604 (3.1%) specimens. By RT-PCR, 331 (54.8%) samples tested positive for DENV; 321 had single infections (DENV-4 n = 305; DENV-1 n = 15; DENV-3 n = 1), nine had co-infections of DENV-1/DENV-4, and one of DENV-2/DENV-4. DENV-4 was detected in 315/331 (95.2%) positive patients from 17 municipalities, and DENV-1 in 24/331 (7.2%) patients from five cities in north-central MT and the city of Cuiaba. The incidence of infection was higher in patients aged 20-39 (142/331; 42.9%). The NS5 partial nucleotide sequence of DENV-1 was most similar to that of genotype V, DENV-2 to Southeast Asian/American, DENV-3 to genotype III, and DENV-4 to genotype II strains, considered the most frequent strains in Brazil. This outbreak coincided with the introduction of DENV-4 in the state. Cuiaba was hyperendemic for the four DENV serotypes, highlighting the necessity for arbovirus surveillance in MT.
authors.affiliation: Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brasil.
authors.forename: Letícia Borges da Silva
authors.initials: LB
authors.lastname: Heinen
authors.affiliation: Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brasil.
authors.forename: Nayara
authors.initials: N
authors.lastname: Zuchi
authors.affiliation: Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brasil.
authors.forename: Belgath Fernandes
authors.initials: BF
authors.lastname: Cardoso
authors.affiliation: Secretaria Estadual de Saúde, Cuiabá, Mato Grosso, Brasil.
authors.forename: Marcelo Adriano Mendes dos
authors.initials: MA
authors.lastname: Santos
authors.affiliation: Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, São Paulo, Brasil.
authors.forename: Mauricio Lacerda
authors.initials: ML
authors.lastname: Nogueira
authors.affiliation: Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brasil.
authors.forename: Renata
authors.initials: R
authors.lastname: Dezengrini-Slhessarenko
mesh_terms: D000293:Adolescent; D000328:Adult; D001938:Brazil / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D003715:Dengue / Q000097:blood / Q000453:epidemiology*; D003716:Dengue Virus / Q000235:genetics*; D004196:Disease Outbreaks / Q000706:statistics & numerical data*; D005260:Female; D005334:Fever / Q000453:epidemiology; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D065288:Serogroup; D055815:Young Adult
chemical_list: D004279:DNA, Viral