title: A novel non-thermostable deuterolysin from Aspergillus oryzae.
abstract: Three putative deuterolysin (EC 3.4.24.29) genes (deuA, deuB, and deuC) were found in the Aspergillus oryzae genome database ( http://www.bio.nite.go.jp/dogan/project/view/AO ). One of these genes, deuA, was corresponding to NpII gene, previously reported. DeuA and DeuB were overexpressed by recombinant A. oryzae and were purified. The degradation profiles against protein substrates of both enzymes were similar, but DeuB showed wider substrate specificity against peptidyl MCA-substrates compared with DeuA. Enzymatic profiles of DeuB except for thermostability also resembled those of DeuA. DeuB was inactivated by heat treatment above 80° C, different from thermostable DeuA. Transcription analysis in wild type A. oryzae showed only deuB was expressed in liquid culture, and the addition of the proteinous substrate upregulated the transcription. Furthermore, the NaNO3 addition seems to eliminate the effect of proteinous substrate for the transcription of deuB.
authors.affiliation: a Department of Applied Biological Chemistry, The Graduate School of Agriculture , Tokyo University of Agriculture and Technology , Fuchu , Tokyo , Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Maeda
authors.affiliation: b Division of Life Science, Graduate School of Agricultural Science , Tohoku University , Sendai , Japan.|c Amano Enzyme Inc , Nagoya , Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Katase
authors.affiliation: a Department of Applied Biological Chemistry, The Graduate School of Agriculture , Tokyo University of Agriculture and Technology , Fuchu , Tokyo , Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sakai
authors.affiliation: a Department of Applied Biological Chemistry, The Graduate School of Agriculture , Tokyo University of Agriculture and Technology , Fuchu , Tokyo , Japan.
authors.forename: Michio
authors.initials: M
authors.lastname: Takeuchi
authors.affiliation: d National Food Research Institute , Tsukuba , Japan.
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Kusumoto
authors.affiliation: c Amano Enzyme Inc , Nagoya , Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Amano
authors.affiliation: e Gekkeikan Sake Co., Ltd , Fushimi-ku, Kyoto , Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ishida
authors.affiliation: b Division of Life Science, Graduate School of Agricultural Science , Tohoku University , Sendai , Japan.
authors.forename: Keietsu
authors.initials: K
authors.lastname: Abe
authors.affiliation: a Department of Applied Biological Chemistry, The Graduate School of Agriculture , Tokyo University of Agriculture and Technology , Fuchu , Tokyo , Japan.
authors.forename: Youhei
authors.initials: Y
authors.lastname: Yamagata
mesh_terms: D001236:Aspergillus oryzae / Q000201:enzymology / Q000235:genetics*; D004795:Enzyme Stability / Q000235:genetics; D005656:Fungal Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D008666:Metalloendopeptidases / Q000235:genetics*; D009566:Nitrates / Q000737:chemistry; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D005656:Fungal Proteins; D009566:Nitrates; C031618:sodium nitrate; D008666:Metalloendopeptidases
keywords: Aspergillus oryzae; deuterolysin; expression specificity; thermostability