title: Lysophosphatidic Acid Receptor Is a Functional Marker of Adult Hippocampal Precursor Cells.
abstract: Here, we show that the lysophosphatidic acid receptor 1 (LPA1) is expressed by a defined population of type 1 stem cells and type 2a precursor cells in the adult mouse dentate gyrus. LPA1, in contrast to Nestin, also marks the quiescent stem cell population. Combining LPA1-GFP with EGFR and prominin-1 expression, we have enabled the prospective separation of both proliferative and non-proliferative precursor cell populations. Transcriptional profiling of the isolated proliferative precursor cells suggested immune mechanisms and cytokine signaling as molecular regulators of adult hippocampal precursor cell proliferation. In addition to LPA1 being a marker of this important stem cell population, we also show that the corresponding ligand LPA is directly involved in the regulation of adult hippocampal precursor cell proliferation and neurogenesis, an effect that can be attributed to LPA signaling via the AKT and MAPK pathways.
authors.affiliation: CRTD - Center for Regenerative Therapies Dresden, Technische Universität Dresden, Dresden 01307, Germany.
authors.forename: Tara L
authors.initials: TL
authors.lastname: Walker
authors.affiliation: CRTD - Center for Regenerative Therapies Dresden, Technische Universität Dresden, Dresden 01307, Germany.
authors.forename: Rupert W
authors.initials: RW
authors.lastname: Overall
authors.affiliation: German Center for Neurodegenerative Diseases (DZNE) Dresden, Dresden 01307, Germany.
authors.forename: Steffen
authors.initials: S
authors.lastname: Vogler
authors.affiliation: Max Planck Institute for Molecular Cell Biology and Genetics, Dresden 01307, Germany.
authors.forename: Alex M
authors.initials: AM
authors.lastname: Sykes
authors.affiliation: CRTD - Center for Regenerative Therapies Dresden, Technische Universität Dresden, Dresden 01307, Germany.
authors.forename: Susann
authors.initials: S
authors.lastname: Ruhwald
authors.affiliation: German Center for Neurodegenerative Diseases (DZNE) Dresden, Dresden 01307, Germany.
authors.forename: Daniela
authors.initials: D
authors.lastname: Lasse
authors.affiliation: CRTD - Center for Regenerative Therapies Dresden, Technische Universität Dresden, Dresden 01307, Germany.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ichwan
authors.affiliation: CRTD - Center for Regenerative Therapies Dresden, Technische Universität Dresden, Dresden 01307, Germany; German Center for Neurodegenerative Diseases (DZNE) Dresden, Dresden 01307, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Fabel
authors.affiliation: CRTD - Center for Regenerative Therapies Dresden, Technische Universität Dresden, Dresden 01307, Germany; German Center for Neurodegenerative Diseases (DZNE) Dresden, Dresden 01307, Germany. Electronic address: gerd.kempermann@crt-dresden.de.
authors.forename: Gerd
authors.initials: G
authors.lastname: Kempermann
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D015153:Blotting, Western; D049109:Cell Proliferation; D002469:Cell Separation; D002478:Cells, Cultured; D018891:Dentate Gyrus / Q000166:cytology / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D063990:Gene Ontology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D008246:Lysophospholipids / Q000494:pharmacology; D008297:Male; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D049368:Receptors, Lysophosphatidic Acid / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012420:Running; D015398:Signal Transduction / Q000187:drug effects; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D008246:Lysophospholipids; D049368:Receptors, Lysophosphatidic Acid; D049452:Green Fluorescent Proteins; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases; C032881:lysophosphatidic acid