title: The Effects of IFN-β 1a on the Expression of Inflammasomes and Apoptosis-Associated Speck-Like Proteins in Multiple Sclerosis Patients.
abstract: This study aims to evaluate the effects of treatment with IFN-β 1α on the expressions of NLRP3, NLRP1, NLRC4, and AIM2, as inflammasomes, and caspase-1, IL-1β, and IL-18, as the downstream molecules of inflammasomes, in a population of Iranian multiple sclerosis (MS) patients. In this study, 30 MS patients (22 women and 8 men) participated. Before receiving any medication and 6Â months after treatment with standard doses of IFN-β 1α 30Â mcg injected intramuscularly once a week, blood samples were taken and then the leukocytes isolated, total RNAs extracted, and complementary DNAs (cDNAs) synthesized. Gene expressions of NLRP3, NLRP1, NLRC4, AIM2, and ASC were evaluated at messenger RNA (mRNA) levels using real-time PCR method; for assessing caspase-1 at protein level, the Western blot method was used. The amounts of IL-1β and IL-18 were measured in plasma using enzyme-linked immunosorbent assay method. Analysis of the results before and after therapy with IFN-β 1α in all patients shows significantly decreased expressions of NLRP3, NLRC4, and AIM2. The plasma levels of IL-1β, after treatment with IFN-β 1α, were significantly decreased in the MS patients. Based on our results, it appears that NLRP3, NLRC4, and AIM2 play critical roles in the progression of MS, probably by mediating Th1 and Th17 responses. It seems that decreased expression of IL-1β is related to decreased production and also functions of inflammasomes.
authors.affiliation: Neurology Research Center, Kerman University of Medical Sciences, Kerman, Iran.|Department of Biochemistry, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Saam
authors.initials: S
authors.lastname: Noroozi
authors.affiliation: Neurology Research Center, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Hossein Ali Ebrahimi
authors.initials: HAE
authors.lastname: Meimand
authors.affiliation: Department of Laboratory Sciences, Faculty of Paramedicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
authors.forename: Mohammad Kazemi
authors.initials: MK
authors.lastname: Arababadi
authors.affiliation: Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Nouzar
authors.initials: N
authors.lastname: Nakhaee
authors.affiliation: Endocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran. gh_asadi@kmu.ac.ir.|Department of Biochemistry, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran. gh_asadi@kmu.ac.ir.
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Asadikaram
mesh_terms: D015153:Blotting, Western; D053468:CARD Signaling Adaptor Proteins; D020170:Caspase 1 / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D058847:Inflammasomes / Q000378:metabolism*; D000068556:Interferon beta-1a / Q000494:pharmacology / Q000627:therapeutic use*; D020382:Interleukin-18 / Q000097:blood; D053583:Interleukin-1beta / Q000097:blood; D008297:Male; D009103:Multiple Sclerosis / Q000097:blood / Q000188:drug therapy* / Q000473:pathology*
chemical_list: D053468:CARD Signaling Adaptor Proteins; D003598:Cytoskeletal Proteins; D058847:Inflammasomes; D020382:Interleukin-18; D053583:Interleukin-1beta; C402202:PYCARD protein, human; D020170:Caspase 1; D000068556:Interferon beta-1a
keywords: Caspase-1; Cytokine; IFN-β 1α; Inflammasomes; Multiple sclerosis