title: Tissue Factor in Dermatitis Herpetiformis and Bullous Pemphigoid: Link between Immune and Coagulation System in Subepidermal Autoimmune Bullous Diseases.
abstract: Dermatitis herpetiformis (DH) and bullous pemphigoid (BP) are skin diseases associated with eosinophilic and neutrophilic infiltrations. Although chemokines are critical for the selective accumulation and activation of various leukocyte subsets in the inflammatory process, there are few findings concerning inflammatory cells and production of coagulation factors in blistering diseases. Skin biopsies were taken from 14 patients with DH, 27 with BP, and 20 control subjects. The localization and expression of tissue factor (TF) in skin lesions and perilesional skin were studied by immunohistochemistry and confirmed by Western Blot. Moreover the plasma concentrations of TF were measured by immunoassays. D dimers, fibrinogen, and selected coagulation parameters were measured by routine methods. Expression of TF in the epidermis and in inflammatory influxed cells in dermis was detected in skin biopsies from BP patients. Examined TF expression was detected in perilesional skin of all BP patients too. The expression of TF was not observed in biopsies from healthy people and DH patients. The findings of the study show an increased expression of tissue factor in the lesional and perilesional skin of patients with bullous pemphigoid. The difference in chemokine pattern expression and variations in the cellular infiltration in BP and DH cause variable expression of TF.
authors.affiliation: Department of Dermatology and Venereology, Medical University of Lodz, Hallera Square 1, 90-497 Lodz, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Zebrowska
authors.affiliation: Laboratory of Nephropathology, Medical University of Lodz, Pomorska 251 Street, 92-213 Lodz, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Wagrowska-Danilewicz
authors.affiliation: Laboratory of Nephropathology, Medical University of Lodz, Pomorska 251 Street, 92-213 Lodz, Poland.
authors.forename: Marian
authors.initials: M
authors.lastname: Danilewicz
authors.affiliation: Department of Immunopathology, Faculty of Biomedical Sciences and Postgraduate Training, Medical University of Lodz, Zeligowskiego 7/9, 90-752 Lodz, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Wieczfinska
authors.affiliation: Department of Immunopathology, Faculty of Biomedical Sciences and Postgraduate Training, Medical University of Lodz, Zeligowskiego 7/9, 90-752 Lodz, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Pniewska
authors.affiliation: Department of Social Medicine, Medical University of Lodz, Zeligowskiego 7/9, 90-752 Lodz, Poland.
authors.forename: Michal
authors.initials: M
authors.lastname: Zebrowski
authors.affiliation: Department of Dermatology and Venereology, Medical University of Lodz, Hallera Square 1, 90-497 Lodz, Poland.
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Waszczykowska
authors.affiliation: Department of Dermatology and Venereology, Medical University of Lodz, Hallera Square 1, 90-497 Lodz, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Wozniacka
authors.affiliation: Department of Laboratory Diagnostics, Medical University of Lodz, Kopcinskiego 22, 90-153 Lodz, Poland.
authors.forename: Makandjou-Ola
authors.initials: M
authors.lastname: Eusebio
authors.affiliation: Department of Laboratory Diagnostics, Medical University of Lodz, Kopcinskiego 22, 90-153 Lodz, Poland.
authors.forename: Miroslawa
authors.initials: M
authors.lastname: Pietruczuk
authors.affiliation: Department of Immunopathology, Faculty of Biomedical Sciences and Postgraduate Training, Medical University of Lodz, Zeligowskiego 7/9, 90-752 Lodz, Poland.
authors.forename: Rafal
authors.initials: R
authors.lastname: Pawliczak
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001777:Blood Coagulation / Q000276:immunology*; D016022:Case-Control Studies; D003874:Dermatitis Herpetiformis / Q000097:blood* / Q000276:immunology* / Q000473:pathology; D005260:Female; D005338:Fibrin Fibrinogen Degradation Products / Q000378:metabolism; D005340:Fibrinogen / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010391:Pemphigoid, Bullous / Q000097:blood* / Q000276:immunology* / Q000473:pathology; D012867:Skin / Q000276:immunology / Q000473:pathology; D013925:Thromboplastin / Q000378:metabolism*; D055815:Young Adult
chemical_list: D005338:Fibrin Fibrinogen Degradation Products; C036309:fibrin fragment D; D005340:Fibrinogen; D013925:Thromboplastin