title: Atomic Force Microscopy of DNA-wrapped Single-walled Carbon Nanotubes in Aqueous Solution.
abstract: We evaluated hybrids of DNA and single-walled carbon nanotubes (SWNTs) in aqueous solution and in air using atomic force microscopy (AFM). Although intensive AFM observations of these hybrids were previously carried out for samples in air, this is the first report on AFM observations of these hybrids in solution. As expected, diameters of DNA-SWNT hybrids dramatically increased in tris(hydroxymethyl)aminomethane-ethylenediaminetetraacetic acid (TE) buffer solution. The data suggest that DNA molecules maintain their structures even on the SWNT surfaces. Furthermore, we simultaneously observed single DNA-SWNT hybrids using three different AFM modes in air and in the TE buffer solution. Height value of the hybrids was largest in the solution, and lowest for the mode that repulsive force is expected in air. For the bare SWNT molecules, height differences among the three AFM modes were much lower than those of the DNA-SWNT hybrids. DNA molecules adsorbed on SWNT surfaces flexibly changed their morphology as well as DNA molecules on flat surfaces such as mica. This is hopeful results for biological applications of DNA-SWNT hybrids. In addition, our results revealed the importance of the single-molecule approach to evaluate DNA structures on SWNT surfaces.
authors.affiliation: Biophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Hayashida
authors.affiliation: Biophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan. Electronic address: meicun2006@163.com.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Umemura
mesh_terms: D000327:Adsorption; D004247:DNA / Q000737:chemistry*; D004492:Edetic Acid / Q000737:chemistry; D018625:Microscopy, Atomic Force; D037742:Nanotubes, Carbon / Q000737:chemistry*; D013499:Surface Properties; D014325:Tromethamine / Q000737:chemistry; D014867:Water / Q000737:chemistry*
chemical_list: D037742:Nanotubes, Carbon; D014325:Tromethamine; D014867:Water; D004247:DNA; D004492:Edetic Acid
keywords: DNA; atomic force microscopy; attractive; flexibility; repulsive; single-walled carbon nanotube