title: Adaptive evolution of Schizochytrium sp. by continuous high oxygen stimulations to enhance docosahexaenoic acid synthesis.
abstract: Adaptive laboratory evolution (ALE) is an effective method in changing the strain characteristics. Here, ALE with high oxygen as a selection pressure was applied to improve the production capacity of Schizochytrium sp. Results showed that cell dry weight (CDW) of endpoint strain was 32.4% higher than that of starting strain. But slight lipid accumulation impairment was observed. These major performance changes were accompanied with enhanced isocitrate dehydrogenase enzyme activity and reduced ATP:citrate lyase enzyme activity. And a serious decrease of 62.6% in SDHA 140rpm→170rpm was observed in the endpoint strain. To further study the docosahexaenoic acid (DHA) production ability of evolved strain, fed-batch strategy was applied and 84.34g/L of cell dry weight and 26.40g/L of DHA yield were observed. In addition, endpoint strain produced greatly less squalene than starting strain. This work demonstrated that ALE may be a promising tool in modifying microalga strains.
authors.affiliation: Jiangsu National Synergetic Innovation Center for Advanced Materials, College of Biotechnology and Pharmaceutical Engineering, School of Pharmaceutical Sciences, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
authors.forename: Xiao-Man
authors.initials: XM
authors.lastname: Sun
authors.affiliation: Jiangsu National Synergetic Innovation Center for Advanced Materials, College of Biotechnology and Pharmaceutical Engineering, School of Pharmaceutical Sciences, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China. Electronic address: renlujing@njtech.edu.cn.
authors.forename: Lu-Jing
authors.initials: LJ
authors.lastname: Ren
authors.affiliation: Jiangsu National Synergetic Innovation Center for Advanced Materials, College of Biotechnology and Pharmaceutical Engineering, School of Pharmaceutical Sciences, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Ji
authors.affiliation: Jiangsu National Synergetic Innovation Center for Advanced Materials, College of Biotechnology and Pharmaceutical Engineering, School of Pharmaceutical Sciences, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
authors.forename: Sheng-Lan
authors.initials: SL
authors.lastname: Chen
authors.affiliation: Jiangsu National Synergetic Innovation Center for Advanced Materials, College of Biotechnology and Pharmaceutical Engineering, School of Pharmaceutical Sciences, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
authors.forename: Dong-Sheng
authors.initials: DS
authors.lastname: Guo
authors.affiliation: Jiangsu National Synergetic Innovation Center for Advanced Materials, College of Biotechnology and Pharmaceutical Engineering, School of Pharmaceutical Sciences, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China. Electronic address: biotech@njtech.edu.cn.
authors.forename: He
authors.initials: H
authors.lastname: Huang
mesh_terms: D004281:Docosahexaenoic Acids / Q000096:biosynthesis*; D005285:Fermentation; D058086:Microalgae / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D010100:Oxygen / Q000494:pharmacology*; D058009:Stramenopiles / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology
chemical_list: D004281:Docosahexaenoic Acids; D010100:Oxygen
keywords: Adaptive evolution; Docosahexaenoic acid; Enzyme activity; Schizochytrium sp