title: Effect of amygdalin on proliferation of rat hepatic fat-storing cells and collagen production in vitro.
abstract: OBJECTIVE
  To study the anti-fibrotic mechanism of amygdalin, a component of Semen Persicae, on fat-storing cells (FSC).
  
  
  METHODS
  Livers of normal adult rats were perfused with Pronas E and collagenase in situ. FSC were isolated by centrifugation with 11% Metrizamide. The subcultured FSC were incubated with 10(-4)-10(-8) mol/L amygdalin for 72 h, and then FSC proliferation and collagen production were assayed, respectively.
  
  
  RESULTS
  Low doses of amygdalin reduced incorporation of L-[(3)H]-thymidine into FSC and L-[5-(3)H]-proline into secreted collagenase-sensitive proteins and cell layer-associated collagenase-sensitive proteins. the strongest effects were seen for the 10(-8) mol/L dose of amygdalin, which inhibited the proliferation of FSC by 25.0%, and decreased collagen production in medium and cell layer by 24.2% and 26.8%, respectively.
  
  
  CONCLUSIONS
  An anti-fibrotic mechanism of amygdalin is to inhibit the proliferation and collagen production of active FSC.
authors.affiliation: Lie-Ming Xu, Cheng Liu, Ping Liu, Liver Diseases Research Center, Shanghai Academy of Traditional Chinese Medicine, Shanghai 200032, China.
authors.forename: L M
authors.initials: LM
authors.lastname: Xu
authors.affiliation: Lie-Ming Xu, Cheng Liu, Ping Liu, Liver Diseases Research Center, Shanghai Academy of Traditional Chinese Medicine, Shanghai 200032, China.
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.affiliation: Lie-Ming Xu, Cheng Liu, Ping Liu, Liver Diseases Research Center, Shanghai Academy of Traditional Chinese Medicine, Shanghai 200032, China.
authors.forename: P
authors.initials: P
authors.lastname: Liu
keywords: Amygdalin; Biosynthesis; Cirrhosis; Collagen; Drug effects; Drug therapy; Liver; Pharmacology