title: Isolation and Propagation of Glioma Stem Cells from Acutely Resected Tumors.
abstract: Gliomas are characterized by striking intratumoral cellular heterogeneity. A consistent method to isolate undifferentiated GSC fraction from clinical specimens provides an opportunity to establish in vitro models that more closely mirror the in vivo state compared to traditional serum containing culture methods. Here we describe techniques involved with isolation, identification, and expansion of human malignant glioma-derived stem cells using a serum-free growth condition.
authors.affiliation: Department of Stem Cell Biology, Cleveland Clinic Foundation, Cleveland, OH, USA.
authors.forename: Jinkyu
authors.initials: J
authors.lastname: Jung
authors.affiliation: Neuro-Oncology Branch, National Cancer Institute, National Institutes of Health, NIH, 9030 Old Georgetown Road, Bethesda, MD, 20817, USA.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Gilbert
authors.affiliation: Neuro-Oncology Branch, National Cancer Institute, National Institutes of Health, NIH, 9030 Old Georgetown Road, Bethesda, MD, 20817, USA. deric.park@nih.gov.
authors.forename: Deric M
authors.initials: DM
authors.lastname: Park
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D002469:Cell Separation / Q000379:methods*; D005910:Glioma / Q000473:pathology* / Q000601:surgery; D006801:Humans; D051379:Mice; D014411:Neoplastic Stem Cells / Q000473:pathology*; D023041:Xenograft Model Antitumor Assays
keywords: GBM; Glioblastoma multiforme; Glioma stem cell; N2 medium; Neural stem cell; Serum-free cell culture