title: CD142+/CD61+, CD146+ and CD45+ microparticles predict cardiovascular events in high risk patients following a Mediterranean diet supplemented with nuts.
abstract: Circulating microparticles (cMPs) are small phospholipid-rich microvesicles shed by activated cells that play a pivotal role in cell signalling related to the pathogenesis of atherothrombosis. We aimed to investigate the prognostic value of cMPs released from different vascular cells for cardiovascular event (CVE) presentation in asymptomatic patients at high cardiovascular risk factors under nutritional and pharmacologic treatment. This is a nested case-control study of 50 patients from the five-year follow-up prospective PREDIMED trial enrolled in the nuts arm of the Mediterranean diet (MedDiet-nuts). We randomly selected 25 patients who had suffered a CVE during follow-up and pair-matched them for sex, age, and classical CV risk factors to 25 patients who remained asymptomatic (no-CVE). Total Annexin V-(AV)+ cMPs and cMPs from cells of the vascular compartment were quantified by flow cytometry at baseline and after one year follow-up. MedDiet-nuts and pharmacological treatment neither modified levels nor source of MP shedding in CVE patients. However, no-CVE patients showed 40-86 % decreased total AV+, PAC-1+/AV+, CD61+/AV+, CD142+/CD61+/AV+, CD62P+/AV+, CD146+/AV+, CD63+/AV+ and CD11a+/AV+ cMPs at one year follow-up (p≤0.046, all). CD142+/CD61+/AV+, CD146+/AV+ and CD45+/AV+ cMPs were decreased in no-CVE patients compared to CVE patients. A ROC-curve clustered model for CD142+/CD61+/AV+, CD45+/AV+ and CD146+/AV+ cMPs predicted a future CVE [p<0.0001, AUC=0.805 (0.672 to 0.938)]. In patients at high CV risk profile treated with a controlled MedDiet supplemented with nuts and receiving up-to-date CV drug treatment, reduced cMPs derived from activated platelets, leukocytes and endothelial cells are predictive of protection against CVE within the next four years.
authors.forename: Gemma
authors.initials: G
authors.lastname: Chiva-Blanch
authors.forename: Javier
authors.initials: J
authors.lastname: Crespo
authors.forename: Rosa
authors.initials: R
authors.lastname: Suades
authors.forename: Gemma
authors.initials: G
authors.lastname: Arderiu
authors.forename: Teresa
authors.initials: T
authors.lastname: Padro
authors.forename: Gemma
authors.initials: G
authors.lastname: Vilahur
authors.forename: Judith
authors.initials: J
authors.lastname: Cubedo
authors.forename: Dolores
authors.initials: D
authors.lastname: Corella
authors.forename: Jordi
authors.initials: J
authors.lastname: Salas-Salvadó
authors.forename: Fernando
authors.initials: F
authors.lastname: Arós
authors.forename: Miguel-Angel
authors.initials: MA
authors.lastname: Martínez-González
authors.forename: Emilio
authors.initials: E
authors.lastname: Ros
authors.forename: Montse
authors.initials: M
authors.lastname: Fitó
authors.forename: Ramon
authors.initials: R
authors.lastname: Estruch
authors.affiliation: Prof. Lina Badimon, Cardiovascular Research Center - CSIC-ICCC, Hospital de la Santa Creu i Sant Pau, Av. S. Antoni M. Claret, 167 Barcelona 08025, Spain, Tel.: +34 93 556 58 80, Fax: +34 93 556 55 59, E-mail: lbadimon@csic-iccc.org.
authors.forename: Lina
authors.initials: L
authors.lastname: Badimon
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D051929:CD146 Antigen / Q000097:blood; D002318:Cardiovascular Diseases / Q000097:blood / Q000209:etiology* / Q000517:prevention & control; D016022:Case-Control Studies; D055252:Cell-Derived Microparticles / Q000276:immunology* / Q000473:pathology*; D038441:Diet, Mediterranean; D005260:Female; D006801:Humans; D039661:Integrin beta3 / Q000097:blood; D017493:Leukocyte Common Antigens / Q000097:blood; D008297:Male; D008875:Middle Aged; D009754:Nuts; D011379:Prognosis; D011446:Prospective Studies; D012307:Risk Factors; D013925:Thromboplastin / Q000378:metabolism
chemical_list: D051929:CD146 Antigen; C061248:ITGB3 protein, human; D039661:Integrin beta3; C497902:MCAM protein, human; D013925:Thromboplastin; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human
keywords: Circulating microparticles; cardiovascular event; leukocytes; platelets; tissue factor