title: A label free electrochemical immunosensor for sensitive detection of porcine serum albumin as a marker for pork adulteration in raw meat.
abstract: A label free electrochemical immunosensor for sensitive detection of porcine serum albumin (PSA) is reported in this work. The immunosensor was constructed by first electrochemically reducing 4-carboxyphenyl diazonium salt, which had been electrochemically generated in situ, to a stable 4-carboxyphenyl layer on carbon nanofiber-modified screen printed electrode. Antibodies were covalently attached onto the electrode using carbodiimide chemistry between the carboxylic groups of the 4-carboxyphenyl layer and amine groups of the antibody. Using the strong affinities of serum albumins towards anions, the increase in cathodic peak current in anionic redox probe after immunocomplex formation with antibodies was used for PSA detection. The reported immunosensor demonstrated a linear range from 0.5 to 500pg/mL for the measurement of PSA with detection limit of 0.5pg/mL in buffer solution. Cross-reactivity studies have shown excellent specificity with satisfactory recovery of PSA in fresh meat samples without the need of sample dilution.
authors.affiliation: Environmental and Life Sciences Programme, Faculty of Science, Universiti Brunei Darussalam, Jalan Tungku Link, Gadong BE 1410, Brunei Darussalam; Biosensors and Biotechnology Laboratory, Chemical Science Programme, Faculty of Science, Universiti Brunei Daruusalam, Jalan Tungku Link, Gadong BE 1410, Brunei Darussalam.
authors.forename: Syazana Abdullah
authors.initials: SA
authors.lastname: Lim
authors.affiliation: Biosensors and Biotechnology Laboratory, Chemical Science Programme, Faculty of Science, Universiti Brunei Daruusalam, Jalan Tungku Link, Gadong BE 1410, Brunei Darussalam. Electronic address: minhaz.ahmed@ubd.edu.bn.
authors.forename: Minhaz Uddin
authors.initials: MU
authors.lastname: Ahmed
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D004566:Electrodes; D005504:Food Analysis; D007118:Immunoassay; D057230:Limit of Detection; D000069466:Red Meat / Q000032:analysis*; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000032:analysis*; D013552:Swine
chemical_list: D012709:Serum Albumin
keywords: Carbon nanofiber; Immunosensor; Meat; Porcine serum albumin