title: Qi-Shen-Yi-Qi Dripping Pills Promote Angiogenesis of Ischemic Cardiac Microvascular Endothelial Cells by Regulating MicroRNA-223-3p Expression.
abstract: Traditional Chinese medicine (TCM) research shows that Qi-Shen-Yi-Qi Dripping Pills (QSYQ) can promote ischemic cardiac angiogenesis. Studies have shown that microRNAs (miRNAs) are the key component of gene regulation networks, which play a vital role in angiogenesis and cardiovascular disease. Mechanisms involving miRNA by which TCM promotes ischemic cardiac angiogenesis have not been reported. We found that microRNA-223-3p (mir-223-3p) was the core miRNA of angiogenesis of rats ischemic cardiac microvascular endothelial cells (CMECs) and inhibited angiogenesis by affecting RPS6KB1/HIF-1α signal pathway in previous study. Based on the results, we observed biological characteristics and optimal dosage for QSYQ intervening in rats ischemic CMECs angiogenesis and concluded that QSYQ low-dose group had the strongest ability to promote angiogenesis of ischemic myocardium. Using miRNA chip and real-time PCR techniques in this study, we identified mir-223-3p as the pivotal miRNA in QSYQ that regulated angiogenesis of ischemic CMECs. From real-time PCR and western blot analysis, research showed that gene and protein expression of factors located RPS6KB1/HIF-1α signaling pathway, including HIF-1α, VEGF, MAPK, PI3K, and AKT, were significantly upregulated by QSYQ to regulate angiogenesis of ischemic CMECs. This study showed that QSYQ promote ischemic cardiac angiogenesis by downregulating mir-223-3p expression in rats ischemic CMECs.
authors.affiliation: Department of Cardiology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, China.
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Dai
authors.affiliation: Department of Cardiology, Jining Hospital of Traditional Chinese Medicine, Jining 272000, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.affiliation: Shandong University of Traditional Chinese Medicine, Jinan 250014, China.
authors.forename: Jing-Wei
authors.initials: JW
authors.lastname: Zhu
authors.affiliation: Shandong University of Traditional Chinese Medicine, Jinan 250014, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yao
authors.affiliation: Department of Cardiology and Brain, Weihai Hospital of Traditional Chinese Medicine, Weihai 264200, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Cardiology and Brain, Weihai Hospital of Traditional Chinese Medicine, Weihai 264200, China.
authors.forename: Pei-Ze
authors.initials: PZ
authors.lastname: Ma
authors.affiliation: Department of Cardiology, Jining Hospital of Traditional Chinese Medicine, Jining 272000, China.
authors.forename: Xian-Bo
authors.initials: XB
authors.lastname: Song