title: Effect of natural and synthetic surfactants on crude oil biodegradation by indigenous strains.
abstract: Hydrocarbon pollution is a worldwide problem. In this study, five surfactants containing SDS, LAS, Brij 30, Tween 80 and biosurfactant were used to evaluate their effect on crude oil biodegradation. Hydrocarbon degrading bacteria were isolated from oil production water. The biosurfactant used was a kind of cyclic lipopeptide produced by Bacillus subtilis strain WU-3. Solubilization test showed all the surfactants could apparently increase the water solubility of crude oil. The microbial adhesion to the hydrocarbon (MATH) test showed surfactants could change cell surface hydrophobicity (CSH) of microbiota, depending on their species and concentrations. Microcalorimetric experiments revealed these surfactants exhibited toxicity to microorganisms at high concentrations (above 1 CMC), except for SDS which showed low antibacterial activity. Surfactant supplementation (about 0.1 and 0.2 CMC) could improve degradation rate of crude oil slightly, while high surfactant concentration (above 1 CMC) may decrease the degradation rate from 50.5% to 28.9%. Those findings of this work could provide guidance for the application of surfactants in bioremediation of oil pollution.
authors.affiliation: National "International Cooperation Based on Environment and Energy" and School of Civil & Environmental Engineering and, University of Science and Technology Beijing, Beijing 100083, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Tian
authors.affiliation: National "International Cooperation Based on Environment and Energy" and School of Civil & Environmental Engineering and, University of Science and Technology Beijing, Beijing 100083, PR China; School of Water Resource and Environmental Engineering, Sino-Hungarian Joint Laboratory of Environmental Science and Health, China University of Geosciences (Beijing), Beijing 100083, PR China. Electronic address: yaojun@ustb.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Yao
authors.affiliation: National "International Cooperation Based on Environment and Energy" and School of Civil & Environmental Engineering and, University of Science and Technology Beijing, Beijing 100083, PR China.
authors.forename: Ruiping
authors.initials: R
authors.lastname: Liu
authors.affiliation: National "International Cooperation Based on Environment and Energy" and School of Civil & Environmental Engineering and, University of Science and Technology Beijing, Beijing 100083, PR China.
authors.forename: Mijia
authors.initials: M
authors.lastname: Zhu
authors.affiliation: National "International Cooperation Based on Environment and Energy" and School of Civil & Environmental Engineering and, University of Science and Technology Beijing, Beijing 100083, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: National "International Cooperation Based on Environment and Energy" and School of Civil & Environmental Engineering and, University of Science and Technology Beijing, Beijing 100083, PR China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Wu
authors.affiliation: School of Resources and Environment, Anqing Normal University, 1318 Jixian North Road, Anqing 246133, PR China. Electronic address: liuhj@aqtc.edu.cn.
authors.forename: Haijun
authors.initials: H
authors.lastname: Liu
mesh_terms: D017738:Alkanesulfonic Acids / Q000737:chemistry; D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D006838:Hydrocarbons / Q000378:metabolism*; D057927:Hydrophobic and Hydrophilic Interactions; D010578:Petroleum / Q000378:metabolism*; D059392:Petroleum Pollution; D000077423:Polidocanol; D011092:Polyethylene Glycols / Q000737:chemistry; D011136:Polysorbates / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D012995:Solubility; D013501:Surface-Active Agents / Q000737:chemistry*; D014871:Water Microbiology; D014873:Water Pollutants / Q000378:metabolism*
chemical_list: D017738:Alkanesulfonic Acids; D006838:Hydrocarbons; D010578:Petroleum; D011136:Polysorbates; D013501:Surface-Active Agents; D014873:Water Pollutants; C030641:alkylbenzyl sulfonic acid; D000077423:Polidocanol; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols
keywords: Biosurfactant; Hydrophobicity; Solubilization; Synthetic surfactant; Toxicity