title: Generation of cloned mice and nuclear transfer embryonic stem cell lines from urine-derived cells.
abstract: Cloning animals by nuclear transfer provides the opportunity to preserve endangered mammalian species. However, there are risks associated with the collection of donor cells from the body such as accidental injury to or death of the animal. Here, we report the production of cloned mice from urine-derived cells collected noninvasively. Most of the urine-derived cells survived and were available as donors for nuclear transfer without any pretreatment. After nuclear transfer, 38-77% of the reconstructed embryos developed to the morula/blastocyst, in which the cell numbers in the inner cell mass and trophectoderm were similar to those of controls. Male and female cloned mice were delivered from cloned embryos transferred to recipient females, and these cloned animals grew to adulthood and delivered pups naturally when mated with each other. The results suggest that these cloned mice had normal fertility. In additional experiments, 26 nuclear transfer embryonic stem cell lines were established from 108 cloned blastocysts derived from four mouse strains including inbreds and F1 hybrids with relatively high success rates. Thus, cells derived from urine, which can be collected noninvasively, may be used in the rescue of endangered mammalian species by using nuclear transfer without causing injury to the animal.
authors.affiliation: Faculty of Life and Environmental Sciences, University of Yamanashi, 4-4-37 Takeda, Kofu-shi, Yamanashi, Japan.|Advanced Biotechnology Center, University of Yamanashi, 4-4-37 Takeda, Kofu-shi, Yamanashi, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Mizutani
authors.affiliation: Faculty of Life and Environmental Sciences, University of Yamanashi, 4-4-37 Takeda, Kofu-shi, Yamanashi, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Torikai
authors.affiliation: Advanced Biotechnology Center, University of Yamanashi, 4-4-37 Takeda, Kofu-shi, Yamanashi, Japan.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Wakayama
authors.affiliation: COC Promotion Center, University of Yamanashi, 4-4-37 Takeda, Kofu-shi, Yamanashi, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nagatomo
authors.affiliation: Faculty of Life and Environmental Sciences, University of Yamanashi, 4-4-37 Takeda, Kofu-shi, Yamanashi, Japan.
authors.forename: Yasuhide
authors.initials: Y
authors.lastname: Ohinata
authors.affiliation: Faculty of Life and Environmental Sciences, University of Yamanashi, 4-4-37 Takeda, Kofu-shi, Yamanashi, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kishigami
authors.affiliation: Faculty of Life and Environmental Sciences, University of Yamanashi, 4-4-37 Takeda, Kofu-shi, Yamanashi, Japan.|Advanced Biotechnology Center, University of Yamanashi, 4-4-37 Takeda, Kofu-shi, Yamanashi, Japan.
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Wakayama
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology; D002460:Cell Line; D019976:Cloning, Organism / Q000379:methods*; D004624:Embryo Transfer; D053595:Embryonic Stem Cells / Q000166:cytology; D005260:Female; D005298:Fertility; D049452:Green Fluorescent Proteins; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D053652:Nuclear Transfer Techniques; D014556:Urine / Q000166:cytology*
chemical_list: D049452:Green Fluorescent Proteins