title: Yeasts isolated from figs (Ficus carica L.) as biocontrol agents of postharvest fruit diseases.
abstract: Fresh fruit is highly perishable during postharvest life, mainly due to fungal growth. Thus, fungal control is an important goal for the fruit industry. In this work, a selection of antagonistic yeasts isolated from fig and breba crops were screened in vitro. The isolated yeasts were challenged with three moulds isolated from decayed figs and breba crops, identified as Penicillium expansum M639 and Cladosporium cladosporioides M310 and M624, and pathogenic moulds Botrytis cinerea CECT20518 and Monilia laxa CA1 from culture collections. Two yeast isolates, Hanseniaspora opuntiae L479 and Metschnikowia pulcherrima L672, were selected for their ability to inhibit the growth of aforementioned moulds. These yeasts reduced the radial growth of moulds on PDA by between 45.23% and 66.09%. Antagonistic activity was associated with the interaction of live yeast cells with moulds. M. pulcherrima L672 apparently parasitised C. cladosporioides isolates. In addition, challenges were assayed using wounded apples and nectarines, with significant reductions in percent infection and lesion size for all moulds tested. To our knowledge, this is the first report identifying H. opuntiae as an antagonist against different pathogenic moulds.
authors.affiliation: Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Av. Adolfo Suárez s/n, 06007 Badajoz, Spain; Instituto Universitario de Recursos Agrarios (INURA), Avd. de la Investigación s/n, Campus Universitario, 06006 Badajoz, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Ruiz-Moyano
authors.affiliation: Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Av. Adolfo Suárez s/n, 06007 Badajoz, Spain; Instituto Universitario de Recursos Agrarios (INURA), Avd. de la Investigación s/n, Campus Universitario, 06006 Badajoz, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Martín
authors.affiliation: Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Av. Adolfo Suárez s/n, 06007 Badajoz, Spain; Instituto Universitario de Recursos Agrarios (INURA), Avd. de la Investigación s/n, Campus Universitario, 06006 Badajoz, Spain.
authors.forename: M C
authors.initials: MC
authors.lastname: Villalobos
authors.affiliation: Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Av. Adolfo Suárez s/n, 06007 Badajoz, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Calle
authors.affiliation: Área de Vegetales, Centro de Investigaciones Científicas y Tecnológicas de Extremadura (CICYTEX), A5 km 372, 06187 Guadajira, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Serradilla
authors.affiliation: Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Av. Adolfo Suárez s/n, 06007 Badajoz, Spain; Instituto Universitario de Recursos Agrarios (INURA), Avd. de la Investigación s/n, Campus Universitario, 06006 Badajoz, Spain.
authors.forename: M G
authors.initials: MG
authors.lastname: Córdoba
authors.affiliation: Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Av. Adolfo Suárez s/n, 06007 Badajoz, Spain; Instituto Universitario de Recursos Agrarios (INURA), Avd. de la Investigación s/n, Campus Universitario, 06006 Badajoz, Spain. Electronic address: ahernandez@unex.es.
authors.forename: A
authors.initials: A
authors.lastname: Hernández
mesh_terms: D000898:Antibiosis; D020171:Botrytis / Q000254:growth & development; D002175:Candida / Q000254:growth & development; D030681:Ficus / Q000382:microbiology*; D005638:Fruit / Q000382:microbiology*; D027845:Malus / Q000382:microbiology*; D010407:Penicillium / Q000254:growth & development; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control*; D057048:Plant Nectar; D015003:Yeasts / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*
chemical_list: D057048:Plant Nectar
keywords: Antagonistic yeast; Botrytis cinerea; Cladosporium cladosporioides; Hanseniaspora opuntiae; Metschnikowia pulcherrima; Monilia laxa; Penicillium expansum; Postharvest disease