title: Glucotoxicity Induced Oxidative Stress and Inflammation In Vivo and In Vitro in Psammomys obesus: Involvement of Aqueous Extract of Brassica rapa rapifera.
abstract: Context. Brassica rapa is considered as natural source of antioxidants and is used to treat diabetes. Objective. Our study carried the impact of glucotoxicity induced in vivo and in vitro in vascular smooth muscle cells (VSMCs) in Psammomys and the therapeutic effect of Brassica rapa (AEBr). Materials and Methods. We administered a hyperglucidic diet (30% sucrose) for 9 months and a treatment for 20 days with AEBr at 100 mg/kg. VSMCs were submitted to D-Glucose (0.6%) for 48 hours and treated with AEBr (2100 μg/mL) for 24 hours. We measured, in blood metabolic parameters, the redox statues and inflammatory markers in adipose tissue. Histological study was effectuated in liver. In VSMCs, we measured markers of glucotoxicity (IRS1p Serine, AKT) inflammation (NO, MCP1, TNFα, and NF-κB) and oxidative stress (oxidants and antioxydants markers). Cell viability and apoptosis were estimated by the morphological study. Results. AEBr corrects the metabolic parameters and inflammatory and oxidative markers in blood and homogenate tissue and reduces lipid droplets in liver. It induces, in VSMCs, a significant decrease of IRS1p serine, cyt c, NO, MCP1, TNFα, NF-κB, protein, and lipid oxidation and increases cell viability, AKT, ERK1/2, catalase, and SOD activity. Conclusion. Brassica enhanced the antidiabetic, anti-inflammatory, and antioxidant defense leading to the protection of cardiovascular diseases.
authors.affiliation: Laboratory of Physiology of Organisms, Team of Cellular and Molecular Physiopathology, Faculty of Biological Sciences, University of Technological Sciences Houari Boumediene, BP 32, EL Alia, 16011 Algiers, Algeria.
authors.forename: Sihem
authors.initials: S
authors.lastname: Berdja
authors.affiliation: Laboratory of Physiology of Organisms, Team of Cellular and Molecular Physiopathology, Faculty of Biological Sciences, University of Technological Sciences Houari Boumediene, BP 32, EL Alia, 16011 Algiers, Algeria.
authors.forename: Leila
authors.initials: L
authors.lastname: Smail
authors.affiliation: Laboratory of Organic and Functionally Analysis, Faculty of Chemistry, USTHB, BP 32, El Alia, 16011 Algiers, Algeria.
authors.forename: Boualem
authors.initials: B
authors.lastname: Saka
authors.affiliation: Laboratory of Physiology of Organisms, Team of Cellular and Molecular Physiopathology, Faculty of Biological Sciences, University of Technological Sciences Houari Boumediene, BP 32, EL Alia, 16011 Algiers, Algeria.
authors.forename: Samia
authors.initials: S
authors.lastname: Neggazi
authors.affiliation: Laboratory of Nuclear Medicine of Central Hospital of Army, Ain Naadja, 16005 Algiers, Algeria.
authors.forename: El-Mehdi
authors.initials: el-M
authors.lastname: Haffaf
authors.affiliation: Team of Biochemistry and Extracellular Matrix Remodeling, Faculty of Biological Sciences, USTHB, BP 32, El Alia, 16011 Algiers, Algeria.
authors.forename: Yasmina
authors.initials: Y
authors.lastname: Benazzoug
authors.affiliation: Laboratory of Biochemistry of Central Hospital of Army, Ain Naadja, 16005 Algiers, Algeria.
authors.forename: Ghouti
authors.initials: G
authors.lastname: Kacimi
authors.affiliation: Laboratory of Organic and Functionally Analysis, Faculty of Chemistry, USTHB, BP 32, El Alia, 16011 Algiers, Algeria.
authors.forename: Lynda
authors.initials: L
authors.lastname: Boudarene
authors.affiliation: Laboratory of Physiology of Organisms, Team of Cellular and Molecular Physiopathology, Faculty of Biological Sciences, University of Technological Sciences Houari Boumediene, BP 32, EL Alia, 16011 Algiers, Algeria.
authors.forename: Souhila
authors.initials: S
authors.lastname: Aouichat Bouguerra