title: Gene expression changes in chicken NLRC5 signal pathway associated with in vitro avian leukosis virus subgroup J infection.
abstract: Nucleotide-binding oligomerization domain-like receptors (NLRs) play a key role in the innate immune response as pattern-recognition receptors. However, the role of NLRC5, which is a member of the NLR family, in NF-κB activation and MHC-I expression remains debatable. Infection with the J group avian leukosis virus (ALV-J) can result in immunosuppression and a subsequent increase in susceptibility to secondary infection. This results in huge economic losses to the poultry industry worldwide. Using quantitative real-time polymerase chain reaction (qRT-PCR), we investigated the mRNA expression levels of NLRC5 signal pathway-related genes in secondary chicken embryo fibroblasts 7 days after infection with ALV-J. The results indicated that, compared with the control groups, the expression levels of TLR7, MHC-I, and IL-18 increased significantly in the infected groups at 7 days post-infection (d.p.i.). The expression levels of NLRC5 and IL-6 were conspicuously downregulated at 7 d.p.i., but the expression levels of NF-κB, STAT1, and STAT3 were not significantly altered. These results suggest that NLRC5 and some genes involved in the NLRC5 pathway play a key role in antiviral immunity, typically the response to ALV-J infection. Moreover, MHC-I expression levels vary between different cell types.
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, China.
authors.forename: L L
authors.initials: LL
authors.lastname: Qiu
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, China.
authors.forename: L
authors.initials: L
authors.lastname: Xu
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, China.
authors.forename: X M
authors.initials: XM
authors.lastname: Guo
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, China.
authors.forename: Z T
authors.initials: ZT
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, China.
authors.forename: F
authors.initials: F
authors.lastname: Wan
authors.affiliation: Poultry Institute, Chinese Academy of Agricultural Science, Yangzhou, Jiangsu, China.
authors.forename: X P
authors.initials: XP
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, China.
authors.forename: G H
authors.initials: GH
authors.lastname: Chen
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, China.
authors.forename: G B
authors.initials: GB
authors.lastname: Chang
mesh_terms: D000818:Animals; D001353:Avian Leukosis / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D002642:Chick Embryo; D016207:Cytokines; D005347:Fibroblasts / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D000070576:NLR Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D050794:STAT1 Transcription Factor / Q000235:genetics / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction; D051199:Toll-Like Receptor 7 / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation; D007378:Interleukins; D000095022:Interferon Lambda
chemical_list: D016207:Cytokines; D015395:Histocompatibility Antigens Class I; D015850:Interleukin-6; D016328:NF-kappa B; D000070576:NLR Proteins; D012333:RNA, Messenger; D050794:STAT1 Transcription Factor; D050796:STAT3 Transcription Factor; D051199:Toll-Like Receptor 7; C511668:interferon-lambda protein, mouse; D007378:Interleukins; D000095022:Interferon Lambda