title: Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance.
abstract: Naturally occurring oxygen tolerant NiFe membrane bound hydrogenases have a conserved catalytic bias towards hydrogen oxidation which limits their technological value. We present an Escherichia coli Hyd-1 amino acid exchange that apparently causes the catalytic rate of H2 production to double but does not impact the O2 tolerance.
authors.affiliation: Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK. alison.parkin@york.ac.uk.
authors.forename: Lindsey A
authors.initials: LA
authors.lastname: Flanagan
authors.affiliation: School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK.
authors.forename: John J
authors.initials: JJ
authors.lastname: Wright
authors.affiliation: School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK.
authors.forename: Maxie M
authors.initials: MM
authors.lastname: Roessler
authors.affiliation: Department of Biology, University of York, York YO10 5DD, UK.
authors.forename: James W
authors.initials: JW
authors.lastname: Moir
authors.affiliation: Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK. alison.parkin@york.ac.uk.
authors.forename: Alison
authors.initials: A
authors.lastname: Parkin
mesh_terms: D055162:Biocatalysis; D006859:Hydrogen / Q000737:chemistry / Q000378:metabolism*; D006864:Hydrogenase / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism*; D015202:Protein Engineering
chemical_list: D006859:Hydrogen; C043296:nickel-iron hydrogenase; D006864:Hydrogenase; D010100:Oxygen