title: BDNF promotes the axonal regrowth after sciatic nerve crush through intrinsic neuronal capability upregulation and distal portion protection.
abstract: Nowadays peripheral nerve injurie occurs more common, the outcome is often poor because of the ineffective treatment. Recent researches revealed the duration of BDNF administration acts a positive role during the nerve regeneration, but its potential mechanisms beneath the behavioral recovery and axonal regrowth after peripheral nerve injury are still controversial. To observe the potential mechanisms we established sciatic nerve injury model and detected the expression of several axonal regeneration and function related genes. The results showed that, BDNF promotes axonal regrowth through increasing the activation of neuronal intrinsic growth capacity and strengthening the deference effects against distal portion atrophy. To further study, we determined the expression of protein associated to neuronal intrinsic growth capacity and investigated the ultrastructure of the distal portion of the injured nerve were analyzed. These data revealed that BDNF triggers multiple effects including neuronal intrinsic growth capacity improvement and distal portion atrophy protection to promote behavioral recovery following sciatic nerve crush injury in mouse.
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu 210029, China; Department of Neurosurgery, The Affiliated Huai'an Hospital of Xuzhou Medical College, No. 62 South Huaihai Road, Huai'an 223002, China.
authors.forename: Jinyu
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Department of Anesthesiology, Huai'an Matenal and Child Health Hospital, Huai'an, Jiangsu 223002, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu 210029, China.
authors.forename: Xiaocheng
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu 210029, China.
authors.forename: Penglai
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu 210029, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu 210029, China. Electronic address: lilixin2@hotmail.com.
authors.forename: Lixin
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology / Q000502:physiology*; D008297:Male; D008810:Mice, Inbred C57BL; D009416:Nerve Regeneration; D009474:Neurons / Q000502:physiology*; D059348:Peripheral Nerve Injuries / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D012584:Sciatic Nerve / Q000293:injuries* / Q000473:pathology / Q000503:physiopathology
chemical_list: D019208:Brain-Derived Neurotrophic Factor
keywords: BDNF; Gap43; SCI; TEM; Toe spreading; cAMP