title: Heterotrophic nitrogen removal by a newly-isolated alkalitolerant microorganism, Serratia marcescens W5.
abstract: A new microbe, Serratia marcescens W5 was successfully isolated. Its feasibility in purification of excessively nitrogen-containing wastewater was evaluated using inorganic nitrogen media. Single factor tests showed that W5 exhibited high ammonium removal rates (above 80%) under different culture conditions (pH 7-10, C/N ratios of 6-20, 15-35°C, 0-2.5% of salinity, respectively). Besides various organic carbon sources, W5 was able to utilize calcium carbonate with 28.05% of ammonium removed. Further experiments indicated that W5 was capable of resisting high-strength ammonium (1200mg/L) with the maximum removal rate of 514.13mgL(-1)d(-1). The nitrogen removal pathway of W5 was also tested, showing that both nitrite and nitrate were efficiently removed only in the presence of ammonium, with hydroxylamine as intermediate, which was different from the conventional nitrogen removal pathway. All the results verified that W5 was a good candidate for the purification of excessively nitrogenous wastewater.
authors.affiliation: College of Marine Life Science, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China.
authors.forename: Teng
authors.initials: T
authors.lastname: Wang
authors.affiliation: College of Marine Life Science, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China.
authors.forename: Qifeng
authors.initials: Q
authors.lastname: Dang
authors.affiliation: College of Marine Life Science, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China. Electronic address: liucsouc@126.com.
authors.forename: Chengsheng
authors.initials: C
authors.lastname: Liu
authors.affiliation: School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China.
authors.forename: Jingquan
authors.initials: J
authors.lastname: Yan
authors.affiliation: Qingdao Aorun Biotechnology Co. Ltd., Room 602, Century Mansion, 39 Donghaixi Road, Qingdao 266071, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Fan
authors.affiliation: The Graduate School of Biotechnology, Korea University, Seoul 136-701, South Korea.
authors.forename: Dongsu
authors.initials: D
authors.lastname: Cha
authors.affiliation: College of Marine Life Science, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Yin
authors.affiliation: College of Marine Life Science, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China.
authors.forename: Yubei
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000332:Aerobiosis; D064751:Ammonium Compounds / Q000378:metabolism; D058440:Denitrification; D052836:Heterotrophic Processes; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D009584:Nitrogen / Q000302:isolation & purification / Q000378:metabolism*; D012706:Serratia marcescens / Q000378:metabolism*; D062065:Wastewater / Q000737:chemistry
chemical_list: D064751:Ammonium Compounds; D009566:Nitrates; D009573:Nitrites; D009584:Nitrogen; D062065:Wastewater
keywords: Alkalitolerant microorganism; Heterotrophic nitrogen removal; Nitrogen removal pathway; Serratia marcescens; Stress resistance