title: Optimization of the production and characterization of lipase from Candida rugosa and Geotrichum candidum in soybean molasses by submerged fermentation.
abstract: This present work describes the production and biochemical characterization of lipase by Candida rugosa and Geotrichum candidum in a culture supplemented with soybean molasses. After optimizing the fermentation times for both microorganisms, the effects of changing the soybean molasses concentration, the fermentative medium pH and the fermentation temperature were evaluated using the Central Composite Planning. When soybean molasses was used at a concentration of 200 g/L at 27 ± 1 °C and pH 3.5, the lipolytic activity measured in the broth was 12.3 U/mL after 12 h for C. rugosa and 11.48 U/mL after 24 h for G. candidum. The molecular masses were 38.3 kDa to G. candidum lipase and 59.7 kDa to C. rugosa lipase, determined by SDS-PAGE. The lipase from both microorganisms exhibited maximal hydrolytic activity at a temperature of 40 °C and were inhibited at pH 10.0. Using different concentration of p-nitrophenylbutyrate (p-NPB), the kinetic parameters were calculated, as follows: the Km of lipase from G. candidum was 465.44 μM and the Vmax 0.384 μmol/min; the Km and Vmax of lipase from C. rugosa were 129.21 μM and 0.034 μmol/min, respectively. Lipases activity were increased by metallic ions Mg(2+) and Na(+) and inhibited by metallic ion Cu(3+).
authors.affiliation: Faculty of Chemical Engineering, Uberlândia Federal University, Av. João Naves de Ávila, 2121, Campus Santa Mônica, Bloco 1K, 38400-902 Uberlândia, MG, Brazil; Instituto de Investigación en Ciencias de la Alimentación CIAL (CSIC-UAM), C/Nicolás Cabrera 9 28049, Cantoblanco, Campus de la Universidad Autónoma de Madrid, Madrid, Spain. Electronic address: wjunioreq@gmail.com.
authors.forename: Wilson Galvão
authors.initials: WG
authors.lastname: de Morais
authors.affiliation: University of São Paulo, Rua Duque de Caxias Norte, 225 - Sala 6 da ZEA, 13635-900 Pirassununga, SP, Brazil.
authors.forename: Eliana Setsuko
authors.initials: ES
authors.lastname: Kamimura
authors.affiliation: Faculty of Chemical Engineering, Uberlândia Federal University, Av. João Naves de Ávila, 2121, Campus Santa Mônica, Bloco 1K, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Eloízio Júlio
authors.initials: EJ
authors.lastname: Ribeiro
authors.affiliation: Instituto de Investigación en Ciencias de la Alimentación CIAL (CSIC-UAM), C/Nicolás Cabrera 9 28049, Cantoblanco, Campus de la Universidad Autónoma de Madrid, Madrid, Spain; Departamento de Engenharia e Tecnologias, Instituto Superior Politecnico de Tecnologías e Ciencias, ISPTEC, Av. Luanda Sul, Rua Lateral Via S10, Talatona, Luanda Sul, Angola.
authors.forename: Benevides Costa
authors.initials: BC
authors.lastname: Pessela
authors.affiliation: Faculty of Chemical Engineering, Uberlândia Federal University, Av. João Naves de Ávila, 2121, Campus Santa Mônica, Bloco 1K, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Vicelma Luiz
authors.initials: VL
authors.lastname: Cardoso
authors.affiliation: Faculty of Chemical Engineering, Uberlândia Federal University, Av. João Naves de Ávila, 2121, Campus Santa Mônica, Bloco 1K, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Miriam Maria
authors.initials: MM
authors.lastname: de Resende
mesh_terms: D002175:Candida / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D005285:Fermentation; D005848:Geotrichum / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D006868:Hydrolysis; D007218:Industrial Microbiology / Q000379:methods; D007700:Kinetics; D008049:Lipase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008965:Molasses / Q000032:analysis; D013025:Glycine max / Q000737:chemistry*; D013696:Temperature
chemical_list: D008049:Lipase
keywords: Candida rugosa; Geotrichum candidum; Lipase; Soybean molasses; Submerged fermentation