title: Turn-on near-infrared electrochemiluminescence sensing of thrombin based on resonance energy transfer between CdTe/CdS coresmall/shellthick quantum dots and gold nanorods.
abstract: Here we designed a near-infrared electrochemiluminescence (NECL) aptasensor for turn-on ultrasensitive determination of thrombin. It was based on the ECL resonance energy transfer (ECL-RET) of CdTe/CdS coresmall/shellthick quantum dots (QDs) to gold nanorods (AuNRs). AuNRs which functioned as ECL acceptors were assembled onto CdTe/CdS film by DNA hybridization between aptamers and their complementary oligonucleotides. In the absence of thrombin, the NECL of QDs was quenched as a result of the ECL-RET of QDs to AuNRs. In the presence of thrombin, the NECL of the system was "turned on" because thrombin can replace the AuNRs onto the QDs film, owing to the specific aptamer-protein affinity interactions. In this way, the increment of ECL intensity and the concentration of thrombin showed a logarithmic linear correlation in the range of 100 aM to 10 fM with a detection limit of 31 aM (S/N=3). Importantly, the developed aptasensor was successfully applied to thrombin sensing in real serum samples.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, PR China; College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, PR China; Hubei Key Laboratory for Processing and Application of Catalytic Materials, College of Chemical Engineering, Huanggang Normal University, Huangzhou 438000, PR China.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, PR China. Electronic address: hyhan@mail.hzau.edu.cn.
authors.forename: Heyou
authors.initials: H
authors.lastname: Han
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D019187:Cadmium Compounds / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007259:Infrared Rays; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D045663:Quantum Dots / Q000737:chemistry* / Q000648:ultrastructure; D013440:Sulfides / Q000737:chemistry; D013691:Tellurium / Q000737:chemistry; D013917:Thrombin / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D019187:Cadmium Compounds; D013440:Sulfides; C034939:cadmium sulfide; C081676:thrombin aptamer; D006046:Gold; D013917:Thrombin; D013691:Tellurium; C028337:cadmium telluride
keywords: Aptasensor; CdTe/CdS quantum dots; Electrochemiluminescence resonance energy transfer; Gold nanorods; Near-infrared; Thrombin