title: Biomechanical Characteristics of Osteoporotic Fracture Healing in Ovariectomized Rats: A Systematic Review.
abstract: Biomechanical tests are widely used in animal studies on osteoporotic fracture healing. However, the biomechanical recovery process is still unknown, leading to difficulty in choosing time points for biomechanical tests and in correctly assessing osteoporotic fracture healing. To determine the biomechanical recovery process during osteoporotic fracture healing, studies on osteoporotic femur fracture healing with biomechanical tests in ovariectomized rat (OVX) models were collected from PUBMED, EMBASE, and Chinese databases. Quadratic curves of fracture healing time and maximum load were fitted with data from the analyzed studies. In the fitted curve for normal fractures, the predicted maximum load was 145.56 N, and the fracture healing time was 88.0 d. In the fitted curve for osteoporotic fractures, the predicted maximum load was 122.30 N, and the fracture healing time was 95.2 d. The maximum load of fractured femurs in OVX rats was also lower than that in sham rats at day 84 post-fracture (D84 PF). The fracture healing time was prolonged and maximum load at D84 PF decreased in OVX rats with closed fractures. The maximum load of Wister rats was higher than that of Sprague-Dawley (SD) rats, but the fracture healing time of SD and Wister rats was similar. Osteoporotic fracture healing was delayed in rats that were < = 12 weeks old when ovariectomized, and at D84 PF, the maximum load of rats < = 12 weeks old at ovariectomy was lower than that of rats >12 weeks old at ovariectomy. There was no significant difference in maximum load at D84 PF between rats with an osteoporosis modeling time <12 weeks and > = 12 weeks. In conclusion, fracture healing was delayed and biomechanical property decreased by osteoporosis. Time points around D95.2 PF should be considered for biomechanical tests of osteoporotic femur fracture healing in OVX rat models. Osteoporotic fracture healing in OVX rats was affected by the fracture type but not by the strain of the rat.
authors.affiliation: Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.|Spine Research Institute, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.|Spine Research Institute, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Long
authors.initials: L
authors.lastname: Yang
authors.affiliation: Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.|Spine Research Institute, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yao
authors.affiliation: Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.|Spine Research Institute, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Cui
authors.affiliation: Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.|Spine Research Institute, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Chun-Chun
authors.initials: CC
authors.lastname: Xue
authors.affiliation: Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.|Spine Research Institute, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Wang
authors.affiliation: Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.|Spine Research Institute, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Shu
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D005260:Female; D005269:Femur / Q000293:injuries; D017102:Fracture Healing; D058866:Osteoporotic Fractures / Q000209:etiology* / Q000628:therapy; D010052:Ovariectomy / Q000009:adverse effects*; D051381:Rats