title: IDENTIFICATION OF THE MINUS MATING-TYPE SPECIFIC GENE MTD1 FROM GONIUM PECTORALE (VOLVOCALES, CHLOROPHYTA)(1).
abstract: Gonium pectorale O. F. Müll. (Volvocales, Chlorophyta), a colonial 8- or 16-cellular alga, is phylogenetically important as an intermediate form between isogametic unicellular Chlamydomonas and oogamous Volvox. We identified the mating-type specific gene GpMTD1, from G. pectorale, the first homologue of Chlamydomonas reinhardtii MTD1 (CrMTD1). The GpMTD1 gene was found to be present only in the minus mating-type locus and was expressed specifically in the gametic phase as is the case for CrMTD1, suggested to participate in development of the minus gametes. This gene is useful as a probe in analyzing the bacterial artificial chromosome (BAC) library for resolving genomic structures of the mating-type loci in isogamous and oogamous colonial volvocaleans.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113-0033, JapanPlant Biology Laboratory, Salk Institute, La Jolla, California 92037, USAAdvanced Science Institute, RIKEN, Wako-shi, Saitama 351-0198, JapanDepartment of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Hamaji
authors.affiliation: Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113-0033, JapanPlant Biology Laboratory, Salk Institute, La Jolla, California 92037, USAAdvanced Science Institute, RIKEN, Wako-shi, Saitama 351-0198, JapanDepartment of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Ferris
authors.affiliation: Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113-0033, JapanPlant Biology Laboratory, Salk Institute, La Jolla, California 92037, USAAdvanced Science Institute, RIKEN, Wako-shi, Saitama 351-0198, JapanDepartment of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Nishii
authors.affiliation: Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113-0033, JapanPlant Biology Laboratory, Salk Institute, La Jolla, California 92037, USAAdvanced Science Institute, RIKEN, Wako-shi, Saitama 351-0198, JapanDepartment of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Hisayoshi
authors.initials: H
authors.lastname: Nozaki
keywords: Gonium pectorale; MID; MTD1; Volvocales; evolution; mating-type locus; sex determination