title: [Mechanism of inhibitory effects of silencing high mobility group box-1 on invasion and migration of  endometrial carcinoma of uterus].
abstract: OBJECTIVE
  To determine the effects of p38 mitogen-activated protein kinase (MAPK) signal pathway after small hairpin RNA on inhibiting the expression of high mobility group box-1 (HMGB1), and to explore the mechanism responsible for the effects of HMGB1 on invasion and migration of endometrial carcinoma of uterus.
  
  
  METHODS
  Based on RNA interference technology, we designed and synthetized HMGB1 small hairpin RNA (HMGB1 shRNA-1, HMGB1 shRNA-2, HMGB1 shRNA-3, HMGB1 shRNA-1), and then transfected HMGB1 shRNA plasmids into HEC-1A cells. The expression of genes and proteins was examined by RT-PCR and Western blot. The activity of HEC-1A cells was evaluated by the methyl thiazolyl tetrazolium (MTT) method.
  
  
  RESULTS
  HMGB1 shRNA effectively inhibited the expression of the HMGB1 and p38MAPK in HEC-1A cells (P<0.05). Among the shRNAs, the inhibitory effect of HMGB1 shRNA-3 was the best (P<0.05). According to the results of MTT, the growth of HEC-1A cells was obviously inhibited after transfection of HEC-1A HMGB1 shRNA (P<0.05).
  
  
  CONCLUSIONS
  Downregulation of HMGB1 may effectively inhibit proliferation, invasion and migration of HEC-1A cells in endometrial carcinoma of uterus, which is dependent on p38MAPK signal pathway.
authors.affiliation: Department of Obstetrics and Gynecology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Jiajie
authors.initials: J
authors.lastname: Wu
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D015536:Down-Regulation; D016889:Endometrial Neoplasms; D005260:Female; D024243:HMGB1 Protein; D006801:Humans; D010957:Plasmids; D034622:RNA Interference; D034741:RNA, Small Interfering; D014162:Transfection; D048051:p38 Mitogen-Activated Protein Kinases
chemical_list: D024243:HMGB1 Protein; C585491:HMGB1 protein, human; D034741:RNA, Small Interfering; D048051:p38 Mitogen-Activated Protein Kinases