title: Enhanced succinic acid production in Corynebacterium glutamicum with increasing the available NADH supply and glucose consumption rate by decreasing H(+)-ATPase activity.
abstract: OBJECTIVE
  To enhance succinic acid production in Corynebacterium glutamicum by increasing the supply of NADH and the rate of glucose consumption by decreasing H(+)-ATPase activity.
  
  
  RESULTS
  A mutant of C. glutamicum NC-3-1 with decreased H(+)-ATPase activity was constructed. This increased the rate of glycolysis and the supply of NADH. Fermentation of C. glutamicum NC-3-1 gave 39 % higher succinic acid production (113 and 81 g/l), a 29 % higher succinic acid yield (0.94 and 0.73 g succinic acid/g glucose) and decreased by-products formation compared to that of C. glutamicum NC-3 in 5 l bioreactor.
  
  
  CONCLUSIONS
  The point mutation in C. glutamicum NC-3-1 increased the rate of glycolysis and resulted in higher succinic acid production, higher succinic acid yield and significantly decreased formation of by-products.
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 211816, China.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Xu
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 211816, China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 211816, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018, China.
authors.forename: Zhongjun
authors.initials: Z
authors.lastname: Chen
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 211816, China. cheng@njtech.edu.cn.
authors.forename: Heng
authors.initials: H
authors.lastname: Cai
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D048230:Corynebacterium glutamicum / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D009243:NAD / Q000378:metabolism*; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D009243:NAD; D019802:Succinic Acid; D000251:Adenosine Triphosphatases; D005947:Glucose
keywords: Corynebacterium glutamicum; Glucose consumption rate; H+-ATPase defect; NADH supply; Succinic acid