title: The effect of explosive blast loading on human neuroblastoma cells.
abstract: Diagnosis of mild to moderate traumatic brain injury is challenging because brain tissue damage progresses slowly and is not readily detectable by conventional imaging techniques. We have developed a novel in vitro model to study primary blast loading on dissociated neurons using nitroamine explosives such as those used on the battlefield. Human neuroblastoma cells were exposed to single and triple 50-psi explosive blasts and single 100-psi blasts. Changes in membrane permeability and oxidative stress showed a significant increase for the single and triple 100-psi blast conditions compared with single 50-psi blast and controls.
authors.affiliation: U.S. Army Research Laboratory, Weapons and Materials Research Directorate, Aberdeen Proving Ground, MD 21005, USA. Electronic address: nicole.e.zander.civ@mail.mil.
authors.forename: Nicole E
authors.initials: NE
authors.lastname: Zander
authors.affiliation: U.S. Army Research Laboratory, Weapons and Materials Research Directorate, Aberdeen Proving Ground, MD 21005, USA.
authors.forename: Thuvan
authors.initials: T
authors.lastname: Piehler
authors.affiliation: U.S. Army Research Laboratory, Weapons and Materials Research Directorate, Aberdeen Proving Ground, MD 21005, USA.
authors.forename: Rohan
authors.initials: R
authors.lastname: Banton
authors.affiliation: Department of Biology, University of Delaware, Newark, DE 19716, USA.
authors.forename: Mary
authors.initials: M
authors.lastname: Boggs
mesh_terms: D001753:Blast Injuries / Q000378:metabolism* / Q000473:pathology*; D000070642:Brain Injuries, Traumatic / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002463:Cell Membrane Permeability; D053834:Explosive Agents; D006801:Humans; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology*; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress
chemical_list: D053834:Explosive Agents
keywords: Explosive blast; In vitro model; Membrane damage; Neurons; Oxidative stress; Traumatic brain injury