title: Genome sequencing of Metrosideros polymorpha (Myrtaceae), a dominant species in various habitats in the Hawaiian Islands with remarkable phenotypic variations.
abstract: Whole genome sequences, which can be provided even for non-model organisms owing to high-throughput sequencers, are valuable in enhancing the understanding of adaptive evolution. Metrosideros polymorpha, a tree species endemic to the Hawaiian Islands, occupies a wide range of ecological habitats and shows remarkable polymorphism in phenotypes among/within populations. The biological functions of genetic variations observed within this species could provide significant insights into the adaptive radiation found in a single species. Here de novo assembled genome sequences of M. polymorpha are presented to reveal basic genomic parameters about this species and to develop our knowledge of ecological divergences. The assembly yielded 304-Mbp genome sequences, half of which were covered by 19 scaffolds with >5 Mbp, and contained 30 K protein-coding genes. Demographic history inferred from the genome-wide heterozygosity indicated that this species experienced a dramatic rise and fall in the effective population size, possibly owing to past geographic or climatic changes in the Hawaiian Islands. This M. polymorpha genome assembly represents a high-quality genome resource useful for future functional analyses of both intra- and interspecies genetic variations or comparative genomics.
authors.affiliation: Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan. izunoay@gmail.com.
authors.forename: Ayako
authors.initials: A
authors.lastname: Izuno
authors.affiliation: Department of Evolutionary Biology and Environmental Studies, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland.|Functional Genomics Center Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland.
authors.forename: Masaomi
authors.initials: M
authors.lastname: Hatakeyama
authors.affiliation: Advanced Science Research Center, Kanazawa University, 13-1 Takara-machi, Kanazawa, 920-0934, Japan.
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Nishiyama
authors.affiliation: Gifu Academy of Forest Science and Culture, 88 Sodai, Mino, Gifu, 501-3714, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Tamaki
authors.affiliation: Department of Evolutionary Biology and Environmental Studies, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland.
authors.forename: Rie
authors.initials: R
authors.lastname: Shimizu-Inatsugi
authors.affiliation: Organization of Frontier Science and Innovation, Kanazawa University, Kakuma-machi, Kanazawa, 920-1192, Japan.
authors.forename: Ryuta
authors.initials: R
authors.lastname: Sasaki
authors.affiliation: Department of Evolutionary Biology and Environmental Studies, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland.
authors.forename: Kentaro K
authors.initials: KK
authors.lastname: Shimizu
authors.affiliation: Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Isagi
mesh_terms: D017753:Ecosystem; D005434:Flow Cytometry; D059646:Genome Size; D018745:Genome, Plant; D006254:Hawaii; D062312:Islands; D027822:Myrtaceae / Q000235:genetics*; D010641:Phenotype; D017422:Sequence Analysis, DNA; D013045:Species Specificity
keywords: Genome; Hawaii; Heterozygosity; Metrosideros; PSMC; Wild plants