title: Gender and chronological age affect erythrocyte membrane oxidative indices in citrate phosphate dextrose adenine-formula 1 (CPDA-1) blood bank storage condition.
abstract: It is well known that in vitro storage lesions lead to membrane dysfunction and decreased number of functional erythrocytes. As erythrocytes get older, in storage media as well as in peripheral circulation, they undergo a variety of biochemical changes. In our study, the erythrocytes with different age groups in citrate phosphate dextrose adenine-formula 1 (CPDA-1) storage solution were used in order to investigate the possible effect of gender factor on oxidative damage. Oxidative damage biomarkers in erythrocyte membranes such as ferric reducing antioxidant power, pro-oxidant-antioxidant balance, protein-bound advance glycation end products, and sialic acid were analyzed. Current study reveals that change in membrane redox status during blood-bank storage condition also depends on both gender depended homeostatic factors and the presence of CPDA-1. During the storage period in CPDA-1, erythrocytes from the male donors are mostly affected by free radical-mediated oxidative stress but erythrocytes obtained from females are severely affected by glyoxidative stress.
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Istanbul Medeniyet University, Goztepe Training and Research Hospital, Istanbul, Turkey. cakatay@yahoo.com.
authors.forename: Hayriye
authors.initials: H
authors.lastname: Erman
authors.forename: Uğur
authors.initials: U
authors.lastname: Aksu
authors.forename: Ahmet
authors.initials: A
authors.lastname: Belce
authors.forename: Pınar
authors.initials: P
authors.lastname: Atukeren
authors.forename: Duygu
authors.initials: D
authors.lastname: Uzun
authors.forename: Tamer
authors.initials: T
authors.lastname: Cebe
authors.forename: Ahmet D
authors.initials: AD
authors.lastname: Kansu
authors.forename: Remisa
authors.initials: R
authors.lastname: Gelişgen
authors.forename: Ezel
authors.initials: E
authors.lastname: Uslu
authors.forename: Seval
authors.initials: S
authors.lastname: Aydın
authors.forename: Ufuk
authors.initials: U
authors.lastname: Çakatay
mesh_terms: D000225:Adenine / Q000737:chemistry*; D000375:Aging / Q000097:blood* / Q000473:pathology*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry; D000094902:Blood Banking / Q000379:methods*; D001793:Blood Preservation / Q000379:methods*; D002951:Citrates / Q000737:chemistry*; D004910:Erythrocyte Membrane / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005947:Glucose / Q000737:chemistry*; D008297:Male; D010084:Oxidation-Reduction; D010710:Phosphates / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012737:Sex Factors
chemical_list: D000975:Antioxidants; C034876:CPDA solutions; D002951:Citrates; D010710:Phosphates; D005947:Glucose; D000225:Adenine