title: Epistatic interactions between at least three loci determine the "rat-tail" phenotype in cattle.
abstract: BACKGROUND
  The "rat-tail" syndrome (RTS) is an inherited hypotrichosis in cattle, which is exclusively expressed in diluted coloured hair. The affected animals also suffer from disturbed thermoregulation, which impairs their health and growth performance. Phenotypic features that are similar to RTS are observed in dogs with black hair follicle dysplasia.
  
  
  RESULTS
  We used a resource cross population between German Holstein and Charolais cattle breeds to prove that epistatic interactions between at least three independent genetic loci are required for the expression of the RTS phenotype. In this population, the RTS is exclusively expressed in animals with a eumelanic background that is due to the dominant E (D) allele at the melanocortin 1 receptor gene located on Bos taurus autosome (BTA) 18. In addition, only the individuals that are heterozygous at the dilution locus on BTA5 that corresponds to the premelanosome protein or silver gene variant c.64G>A were classified as displaying a RTS phenotype. Linkage and whole-genome association analyses using different models and different pedigrees allowed us to map a third locus (hereafter referred to as the RTS locus) that is essential for the expression of the RTS phenotype to the chromosomal region between 14 and 22 Mb on BTA5. Our findings clearly demonstrate that the RTS and dilution loci are distinct loci on BTA5.
  
  
  CONCLUSIONS
  Our study provides evidence that the RTS locus has effects on hair conformation and coat colour dilution and that the effect on coat colour dilution is clearly independent from that of the dilution locus. Finally, our results excluded several other loci that were previously reported to be associated with or to underlie hair conformation or pigmentation traits as the causal mutations of RTS and also several major functional candidate genes that are associated with hypotrichosis in humans. Our finding on the identification of a three-locus interaction that underlies RTS provides a prime example of epistatic interaction between several independent loci that is required for the expression of a distinct phenotype.
authors.affiliation: Institute for Genome Biology, Leibniz Institute for Farm Animal Biology, 18196, Dummerstorf, Mecklenburg-Vorpommern, Germany.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Knaust
authors.affiliation: Institute for Genome Biology, Leibniz Institute for Farm Animal Biology, 18196, Dummerstorf, Mecklenburg-Vorpommern, Germany.
authors.forename: Frieder
authors.initials: F
authors.lastname: Hadlich
authors.affiliation: Institute for Genome Biology, Leibniz Institute for Farm Animal Biology, 18196, Dummerstorf, Mecklenburg-Vorpommern, Germany.
authors.forename: Rosemarie
authors.initials: R
authors.lastname: Weikard
authors.affiliation: Institute for Genome Biology, Leibniz Institute for Farm Animal Biology, 18196, Dummerstorf, Mecklenburg-Vorpommern, Germany. kuehn@fbn-dummerstorf.de.|Faculty of Agricultural and Environmental Sciences, University of Rostock, 18059, Rostock, Germany. kuehn@fbn-dummerstorf.de.
authors.forename: Christa
authors.initials: C
authors.lastname: Kuehn
mesh_terms: D000818:Animals; D001947:Breeding; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics*; D002874:Chromosome Mapping; D004843:Epistasis, Genetic; D008040:Genetic Linkage; D055106:Genome-Wide Association Study / Q000379:methods; D005838:Genotype; D007039:Hypotrichosis / Q000235:genetics / Q000662:veterinary*; D010641:Phenotype; D010858:Pigmentation / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D044102:Receptor, Melanocortin, Type 1 / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D044102:Receptor, Melanocortin, Type 1