title: Construction of SET overexpression vector and its effects on the proliferation and apoptosis of 293T cells.
abstract: The expression of SET nuclear proto‑oncogene (SET) is commonly associated with cell proliferation and tumorigenesis. In the present study, a eukaryotic SET expression plasmid (pEGFP‑N1‑SET) was constructed and transiently transfected into 293T human embryonic kidney cells. Transfection led to expression of the SET oncoprotein at high levels, as indicated by polymerase chain reaction and western blot analysis. In addition, the relative mRNA and protein expression of protein phosphatase 2A in pEGFP‑N1‑SET‑transfected 293T cells was downregulated compared with that in empty vector‑transfected cells. Furthermore, overexpression of SET increased the percentage of 293T cells in S and G2/M phases compared with the control transfectants. An increase in B‑cell lymphoma 2 (Bcl‑2) and a decrease in Bcl‑2‑associated X (Bax) protein expression was observed in the pEGFP‑N1‑SET‑transfected cells compared with that in the controls, and their susceptibility to As4S4‑induced apoptosis was decreased. The protein SET is involved in a number of cellular processes, including DNA replication, chromatin remodeling, gene transcription, differentiation, migration and cell cycle regulation. SET is overexpressed in several neoplasms, particularly in acute myeloid leukemia. The findings of the present study suggested that the SET gene may contribute to tumorigenesis and may be a potential novel effective therapeutic target for leukemia and other cancer types.
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Xi'an Jiaotong University, Medical College, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Xi'an Jiaotong University, Medical College, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Peng-Cheng
authors.initials: PC
authors.lastname: He
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Xi'an Jiaotong University, Medical College, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Yan-Feng
authors.initials: YF
authors.lastname: Liu
authors.affiliation: Institute of Xi'an Blood Bank, Shaanxi Blood Center, Xi'an, Shaanxi 710068, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Qi
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Xi'an Jiaotong University, Medical College, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis; D002460:Cell Line; D049109:Cell Proliferation; D004268:DNA-Binding Proteins; D015870:Gene Expression; D005822:Genetic Vectors; D056488:Histone Chaperones / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D056488:Histone Chaperones; C513473:SET protein, human; D014157:Transcription Factors