title: Human Umbilical Cord Mesenchymal Stem Cells Therapy in Cyclophosphamide-Induced Premature Ovarian Failure Rat Model.
abstract: Premature ovarian failure (POF) is one of the most common causes of infertility in women. In our present study, we established cyclophosphamide- (CTX-) induced POF rat model and elucidated its effect on ovarian function. We detected the serum estrogen, follicle stimulating hormone, and anti-Müllerian hormone of mice models by ELISA and evaluated their folliculogenesis by histopathology examination. Our study revealed that CTX administration could severely disturb hormone secretion and influence folliculogenesis in rat. This study also detected ovarian cells apoptosis by deoxy-UTP-digoxigenin nick end labeling (TUNEL) and demonstrated marked ovarian cells apoptosis in rat models following CTX administration. In order to explore the potential of human umbilical cord mesenchymal stem cells (UCMSCs) in POF treatment, the above indexes were used to evaluate ovarian function. We found that human UCMSCs transplantation recovered disturbed hormone secretion and folliculogenesis in POF rat, in addition to reduced ovarian cell apoptosis. We also tracked transplanted UCMSCs in ovaries by fluorescence in situ hybridization (FISH). The results manifested that the transplanted human UCMSCs could reside in ovarian tissues and could survive for a comparatively long time without obvious proliferation. Our present study provides new insights into the great clinical potential of human UCMSCs in POF treatment.
authors.affiliation: Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Song
authors.affiliation: Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
authors.forename: Chunfeng
authors.initials: C
authors.lastname: Qian
authors.affiliation: Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
authors.forename: Qinyan
authors.initials: Q
authors.lastname: Zou
authors.affiliation: Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Ou
authors.affiliation: Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
authors.forename: Yichao
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Gao
authors.affiliation: Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
authors.forename: Gaigai
authors.initials: G
authors.lastname: Wang
authors.affiliation: Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
authors.forename: Zhenxing
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Li
authors.affiliation: Jiangsu Beike Biotechnology Co., Ltd., Jiangsu 215002, China.
authors.forename: Hailei
authors.initials: H
authors.lastname: Ding
authors.affiliation: Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
authors.forename: Huihua
authors.initials: H
authors.lastname: Wu
authors.affiliation: Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
authors.forename: Fuxin
authors.initials: F
authors.lastname: Wang
authors.affiliation: Experimental Animal Center, Soochow University, Suzhou, Jiangsu 215006, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
authors.forename: Hong
authors.identifier: 0000-0002-6186-2152
authors.initials: H
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D003520:Cyclophosphamide / Q000633:toxicity; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D051379:Mice; D010053:Ovary / Q000473:pathology; D016649:Primary Ovarian Insufficiency / Q000139:chemically induced / Q000601:surgery*; D051381:Rats; D017208:Rats, Wistar; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D003520:Cyclophosphamide