title: Anti-Inflammatory Effect of Quercetin on RAW 264.7 Mouse Macrophages Induced with Polyinosinic-Polycytidylic Acid.
abstract: Quercetin (3,3',4',5,6-pentahydroxyflavone) is a well-known antioxidant and a flavonol found in many fruits, leaves, and vegetables. Quercetin also has known anti-inflammatory effects on lipopolysaccharide-induced macrophages. However, the effects of quercetin on virus-induced macrophages have not been fully reported. In this study, the anti-inflammatory effect of quercetin on double-stranded RNA (dsRNA)-induced macrophages was examined. Quercetin at concentrations up to 50 μM significantly inhibited the production of NO, IL-6, MCP-1, IP-10, RANTES, GM-CSF, G-CSF, TNF-α, LIF, LIX, and VEGF as well as calcium release in dsRNA (50 μg/mL of polyinosinic-polycytidylic acid)-induced RAW 264.7 mouse macrophages (p < 0.05). Quercetin at concentrations up to 50 μM also significantly inhibited mRNA expression of signal transducer and activated transcription 1 (STAT1) and STAT3 in dsRNA-induced RAW 264.7 cells (p < 0.05). In conclusion, quercetin had alleviating effects on viral inflammation based on inhibition of NO, cytokines, chemokines, and growth factors in dsRNA-induced macrophages via the calcium-STAT pathway.
authors.affiliation: Department of Pathology, College of Korean Medicine, Gachon University, Seong-nam 13120, Korea. godsentry@naver.com.
authors.forename: Young-Jin
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Department of Pathology, College of Korean Medicine, Gachon University, Seong-nam 13120, Korea. pws98@gachon.ac.kr.
authors.forename: Wansu
authors.initials: W
authors.lastname: Park
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D018925:Chemokines / Q000096:biosynthesis; D016207:Cytokines / Q000096:biosynthesis; D005786:Gene Expression Regulation / Q000187:drug effects; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis*; D011070:Poly I-C / Q000633:toxicity; D011794:Quercetin / Q000008:administration & dosage*; D000067996:RAW 264.7 Cells
chemical_list: D000975:Antioxidants; D018925:Chemokines; D016207:Cytokines; D009569:Nitric Oxide; D011794:Quercetin; D011070:Poly I-C
keywords: STAT; calcium; cytokine; dsRNA; inflammation; macrophages; nitric oxide; quercetin