title: [The specific features of the distribution of 2,6-di-tret-buthyl-4-methlhydroxybenzole in the organism of the warm-blooded animals].
abstract: We have studied the specific features of the distribution of 2,6-di-tret-buthyl-4-methlhydroxybenzole in the organism of the omnivorous warm-blooded animals (rats) following the intragastric administration of the three-fold lethal dose of the poisonous substance. 2,6-di-tret-buthyl-4-methlhydroxybenzole was isolated from the blood and various organs of the animals by means of acetone extraction. It was further purified on the 40/100 mcm L silicagel column with the use of hexane/acetone for elution. TLC, GC-MS, and UV-spectrophotometry were employed to identify and quantify the material of interest. It was shown that 2,6-di-tret-buthyl-4-methlhydroxybenzole undergoes modification in the internal organs and blood of the poisoned animals. In was present in the highest amounts (100 mg/g) in the stomach and small intestine contents (787.78±52.31 and 18.31±3.47 respectively), spleen (12.46±1.02), and kidneys (8.48±0.61).
authors.affiliation: State budgetary educational institution of higher professional education "Kursk State Medical University", Russian Ministry of Health, Kursk, Russia, 305041.
authors.forename: V K
authors.initials: VK
authors.lastname: Shormanov
authors.affiliation: State budgetary educational institution of higher professional education "Kursk State Medical University", Russian Ministry of Health, Kursk, Russia, 305041.
authors.forename: O I
authors.initials: OI
authors.lastname: Pugacheva
authors.affiliation: State budgetary educational institution of higher professional education "Kursk State Medical University", Russian Ministry of Health, Kursk, Russia, 305041.
authors.forename: A P
authors.initials: AP
authors.lastname: Astashkina
authors.affiliation: State budgetary educational institution of higher professional education "Kursk State Medical University", Russian Ministry of Health, Kursk, Russia, 305041.
authors.forename: E P
authors.initials: EP
authors.lastname: Tsatsua
mesh_terms: D000818:Animals; D002855:Chromatography, Thin Layer / Q000379:methods; D004195:Disease Models, Animal; D053593:Forensic Toxicology / Q000379:methods; D007422:Intestines / Q000473:pathology*; D007668:Kidney / Q000473:pathology*; D007928:Lethal Dose 50; D008297:Male; D010636:Phenols / Q000493:pharmacokinetics*; D011041:Poisoning / Q000209:etiology / Q000473:pathology; D051381:Rats; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013154:Spleen / Q000473:pathology*; D013270:Stomach / Q000473:pathology*; D014018:Tissue Distribution
chemical_list: D010636:Phenols