title: Tracking of Normal and Malignant Progenitor Cell Cycle Transit in a Defined Niche.
abstract: While implicated in therapeutic resistance, malignant progenitor cell cycle kinetics have been difficult to quantify in real-time. We developed an efficient lentiviral bicistronic fluorescent, ubiquitination-based cell cycle indicator reporter (Fucci2BL) to image live single progenitors on a defined niche coupled with cell cycle gene expression analysis. We have identified key differences in cell cycle regulatory gene expression and transit times between normal and chronic myeloid leukemia progenitors that may inform cancer stem cell eradication strategies.
authors.affiliation: Divisions of Regenerative Medicine and Hematology-Oncology, Department of Medicine, Moores Cancer Center, University of California, San Diego, La Jolla, CA 92093-0820, USA.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Pineda
authors.affiliation: Divisions of Regenerative Medicine and Hematology-Oncology, Department of Medicine, Moores Cancer Center, University of California, San Diego, La Jolla, CA 92093-0820, USA.
authors.forename: Kathleen M
authors.initials: KM
authors.lastname: Lennon
authors.affiliation: Divisions of Regenerative Medicine and Hematology-Oncology, Department of Medicine, Moores Cancer Center, University of California, San Diego, La Jolla, CA 92093-0820, USA.
authors.forename: Nathaniel P
authors.initials: NP
authors.lastname: Delos Santos
authors.affiliation: Divisions of Regenerative Medicine and Hematology-Oncology, Department of Medicine, Moores Cancer Center, University of California, San Diego, La Jolla, CA 92093-0820, USA.
authors.forename: Florence
authors.initials: F
authors.lastname: Lambert-Fliszar
authors.affiliation: Divisions of Regenerative Medicine and Hematology-Oncology, Department of Medicine, Moores Cancer Center, University of California, San Diego, La Jolla, CA 92093-0820, USA.|Biological Sciences Department, California Polytechnic State University, San Luis Obispo, CA, 93407, USA.
authors.forename: Gennarina L
authors.initials: GL
authors.lastname: Riso
authors.affiliation: Divisions of Regenerative Medicine and Hematology-Oncology, Department of Medicine, Moores Cancer Center, University of California, San Diego, La Jolla, CA 92093-0820, USA.|Doctoral School of Molecular and Translational Medicine, Department of Health Sciences, University of Milan, Milan, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Lazzari
authors.affiliation: Canada's Michael Smith Genome Sciences Centre, BC Cancer Agency, Vancouver, BC, Canada.
authors.forename: Marco A
authors.initials: MA
authors.lastname: Marra
authors.affiliation: Divisions of Regenerative Medicine and Hematology-Oncology, Department of Medicine, Moores Cancer Center, University of California, San Diego, La Jolla, CA 92093-0820, USA.
authors.forename: Sheldon
authors.initials: S
authors.lastname: Morris
authors.affiliation: Laboratory for Cell Function and Dynamics, Brain Science Institute, RIKEN, Wako-city, Saitama, Japan.
authors.forename: Asako
authors.initials: A
authors.lastname: Sakaue-Sawano
authors.affiliation: Laboratory for Cell Function and Dynamics, Brain Science Institute, RIKEN, Wako-city, Saitama, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Miyawaki
authors.affiliation: Divisions of Regenerative Medicine and Hematology-Oncology, Department of Medicine, Moores Cancer Center, University of California, San Diego, La Jolla, CA 92093-0820, USA.
authors.forename: Catriona H M
authors.initials: CH
authors.lastname: Jamieson
mesh_terms: D002453:Cell Cycle; D002460:Cell Line; D018920:Coculture Techniques; D019008:Drug Resistance, Neoplasm; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007700:Kinetics; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000000981:diagnostic imaging* / Q000235:genetics* / Q000378:metabolism; D018613:Microscopy, Confocal; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D017423:Sequence Analysis, RNA / Q000379:methods*; D055153:Stem Cell Niche; D059008:Time-Lapse Imaging