title: Conventional and fluorescent based semen quality assessment in Karan Fries bulls.
abstract: OBJECTIVE
  The present study was carried out on semen ejaculates of 15 Karan Fries (KF) bulls maintained at Artificial Breeding Research Centre, National Dairy Research Institute, Karnal, India with an objective to evaluate the relationship between the conventional and fluorescent based semen quality analysis of the bulls.
  
  
  METHODS
  A total of 96 ejaculates were collected from 15 KF (Holstein Friesian [HF] crossbred) bulls. Semen were evaluated for color, volume, mass activity (MA) and percentage of individual motility (IM), sperm concentration, percent live spermatozoa, hypo-osmotic swelling test and acrosome integrity, chromatin integrity, sperm viability, and membrane integrity. Data were analyzed using SPSS software package for descriptive analysis. The correlation between rankings of sires based on conventional and fluorescent semen parameters were calculated by Spearman's rank correlation coefficient.
  
  
  RESULTS
  The average ejaculates volume (ml), sperm concentration (10(6)/ml), MA, IM (%), live (%), morphological abnormalities (%), host (%), acrosome integrity (%), chromomycin A3 (CMA3) (%), SYBR-PI (%), and fluorescent isothiocyanate-peanut agglutinin (FITC-PNA) (%) were 4.57±0.36, 1162.98±97.93, 2.95±0.09, 60.8±1.22, 71.41±2.10, 9.31±1.15, 65.5±1.81, 86.6±1.59, 3.53±0.43, 65.39±2.23 and 74.47±2.53, respectively. Rank correlations were found to be significant for SYBR-PI and FITC-PNA with most of the parameters evaluated by conventional methods. Overall, among conventional criteria, IM revealed ranking of bulls almost similar to that of fluorescent criteria.
  
  
  CONCLUSIONS
  Overview of our results indicated that, among conventional criteria, MA and IM revealed ranking of bulls almost similar to that of fluorescent criteria.
authors.affiliation: Division of Dairy Cattle Breeding, Indian Council of Agricultural Research, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: A
authors.initials: A
authors.lastname: Panmei
authors.affiliation: Division of Dairy Cattle Breeding, Indian Council of Agricultural Research, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Gupta
authors.affiliation: Division of Dairy Cattle Breeding, Indian Council of Agricultural Research, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: P R
authors.initials: PR
authors.lastname: Shivahre
authors.affiliation: Division of Dairy Cattle Breeding, Indian Council of Agricultural Research, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: M
authors.initials: M
authors.lastname: Bhakat
authors.affiliation: Division of Dairy Cattle Breeding, Indian Council of Agricultural Research, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: A
authors.initials: A
authors.lastname: Upadhyay
keywords: Karan Fries; conventional method; fluorescent technique; semen quality