title: Effects of Hepatoprotective Compounds from the Leaves of Lumnitzera racemosa on Acetaminophen-Induced Liver Damage in Vitro.
abstract: Phytochemical investigation of the n-BuOH fraction of the mangrove plant Lumnitzera racemosa WILLD. (Combretaceae) led to the isolation of one new flavonoid glycoside; myrcetin 3-O-methyl glucuronate (1), one new phenolic glycoside; lumniracemoside (2) and one new aliphatic alcohol glycoside; n-hexanol 1-O-rutinoside (3), in addition to seven known compounds (4-10). The structures of these compounds were determined by spectroscopic analyses (UV, IR, high resolution-electrospray ionization (HR-ESI)-MS, one- and two-dimensional (1D- and 2D)-NMR). Compound 7 showed the highest hepatoprotective activity against acetaminophen-induced hepatotoxicity using human HepG2 cells at protection % value of 34.2±3.1%, while compounds 1, 2, 3, 6, and 9 showed weak to moderate hepatoprotective activity (11.6-18.9%). Almost all of these compounds showed stronger 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity compared with the standard Trolox. These results suggest the usefulness of this plant extract and the isolated compounds as promising hepatoprotective agents.
authors.affiliation: Department of Pharmacognosy, Graduate School of Biomedical and Health Sciences, Hiroshima University.
authors.forename: Ahmed Gomaa Gomaa
authors.initials: AG
authors.lastname: Darwish
authors.forename: Mamdouh Nabil
authors.initials: MN
authors.lastname: Samy
authors.forename: Sachiko
authors.initials: S
authors.lastname: Sugimoto
authors.forename: Hideaki
authors.initials: H
authors.lastname: Otsuka
authors.forename: Hosni
authors.initials: H
authors.lastname: Abdel-Salam
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Matsunami
mesh_terms: D000082:Acetaminophen / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control*; D029764:Combretaceae / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects*; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D010936:Plant Extracts; D000082:Acetaminophen