title: Preparation and characterization of an advanced collagen aggregate from porcine acellular dermal matrix.
abstract: The objective of this study was to extract and characterize an advanced collagen aggregate (Ag-col) from porcine acellular dermal matrix (pADM). Based on histological examination, scanning electron microscopy (SEM) and atomic force microscope (AFM), Ag-col was composed of the D-periodic cross-striated collagen fibrils and thick collagen fiber bundles with uneven diameters and non-orientated arrangement. Fourier transform infrared (FTIR) spectra of pADM, Ag-col and Col were similar and revealed the presence of the triple helix. Circular dichroism (CD) analysis exhibited a slightly higher content of α-helix but inappreciably less amount of random coil structure in Ag-col compared to Col. Moreover, imino acid contents of pADM, Ag-col and Col were 222.43, 218.30 and 190.01 residues/1000 residues, respectively. From zeta potential analysis, a net charge of zero was found at pH 6.45 and 6.11 for Ag-col and Col, respectively. Differential scanning calorimetry (DSC) study suggested that the Td of Ag-col was 20°C higher than that of Col as expected, and dynamic mechanical analysis (DMA) indicated that Ag-col possessed a higher storage modulus but similar loss factor compared to Col. Therefore, the collagen aggregate from pADM could serve as a better alternative source of collagens for further applications in food and biological industries.
authors.affiliation: Key Laboratory for Leather Chemistry and Engineering of the Education Ministry, Sichuan University, Chengdu, Sichuan 610065, China; Research Center of Biomedical Engineering, Sichuan University, Chengdu, Sichuan 610065, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory for Leather Chemistry and Engineering of the Education Ministry, Sichuan University, Chengdu, Sichuan 610065, China; Research Center of Biomedical Engineering, Sichuan University, Chengdu, Sichuan 610065, China.
authors.forename: Nianhua
authors.initials: N
authors.lastname: Dan
authors.affiliation: Key Laboratory for Leather Chemistry and Engineering of the Education Ministry, Sichuan University, Chengdu, Sichuan 610065, China; Research Center of Biomedical Engineering, Sichuan University, Chengdu, Sichuan 610065, China. Electronic address: danweihua_scu@126.com.
authors.forename: Weihua
authors.initials: W
authors.lastname: Dan
mesh_terms: D062088:Acellular Dermis; D000818:Animals; D003094:Collagen / Q000737:chemistry* / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D066329:Protein Aggregates; D017433:Protein Structure, Secondary; D055672:Static Electricity; D013499:Surface Properties; D013552:Swine; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D066329:Protein Aggregates; D003094:Collagen
keywords: Collagen; Collagen aggregate; Structure