title: Growing evidence suggests WT1 effects in the kidney development are modulated by Hsp70/NO interaction.
abstract: The study of kidney development at the cellular and molecular levels remains an active area of nephrology research. The functional integrity of the kidney depends on normal development as well as on physiological cell turnover. Apoptosis induction is essential for these mechanisms. A route to cell death revealed in the past decade shows that heat shock proteins (HSPs) and their cofactors are responsible for regulating the apoptotic pathway. Specifically, heat shock protein 70 (Hsp70), the most ubiquitous and highly conserved HSP, helps proteins adopt native conformation or regain function after misfolding. Hsp70 is an important cofactor for the function of Wilms' tumour 1 (WT1) and suggests a potential role for this chaperone during kidney differentiation. In addition, we have demonstrated that WT1 expression is modulated by nitric oxide (NO) availability and Hsp70 interaction after neonatal unilateral ureteral obstruction. NO has been identified as playing an important role in the developing kidney. These findings suggest that Hsp70 and NO may play a critical and fundamental role in the capacity to modulate both apoptotic pathway and oxidative stress during kidney development. Furthermore, the design of experimental protocols that assess renal epithelial functionality in this context, could contribute to the understanding of renal development and alterations.
authors.affiliation: Área de Farmacología, Departamento de Patología, Facultad de Ciencias Médicas, Centro Universitario, Universidad Nacional de Cuyo, CP 5500, Mendoza, Argentina.|IMBECU-CONICET (National Council of Scientific and Technical Research of Argentina), Buenos Aires, Argentina.
authors.forename: Luciana
authors.initials: L
authors.lastname: Mazzei
authors.affiliation: Área de Farmacología, Departamento de Patología, Facultad de Ciencias Médicas, Centro Universitario, Universidad Nacional de Cuyo, CP 5500, Mendoza, Argentina. wmanucha@yahoo.com.ar.|IMBECU-CONICET (National Council of Scientific and Technical Research of Argentina), Buenos Aires, Argentina. wmanucha@yahoo.com.ar.
authors.forename: Walter
authors.initials: W
authors.lastname: Manucha
mesh_terms: D000328:Adult; D000818:Animals; D017209:Apoptosis; D058750:Epithelial-Mesenchymal Transition; D018840:HSP70 Heat-Shock Proteins / Q000502:physiology*; D050884:HSP72 Heat-Shock Proteins / Q000502:physiology; D006801:Humans; D007668:Kidney / Q000196:embryology*; D009569:Nitric Oxide / Q000502:physiology*; D018384:Oxidative Stress; D025721:WT1 Proteins / Q000502:physiology*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D050884:HSP72 Heat-Shock Proteins; D025721:WT1 Proteins; C583751:WT1 protein, human; D009569:Nitric Oxide
keywords: Apoptosis; Heat shock protein 70; Kidney development; Nitric oxide; Wilms’ tumour 1