title: MAL62 overexpression and NTH1 deletion enhance the freezing tolerance and fermentation capacity of the baker's yeast in lean dough.
abstract: BACKGROUND
  Trehalose is related to several types of stress responses, especially freezing response in baker's yeast (Saccharomyces cerevisiae). It is desirable to manipulate trehalose-related genes to create yeast strains that better tolerate freezing-thaw stress with improved fermentation capacity, which are in high demand in the baking industry.
  
  
  RESULTS
  The strain overexpressing MAL62 gene showed increased trehalose content and cell viability after prefermention-freezing and long-term frozen. Deletion of NTH1 in combination of MAL62 overexpression further strengthens freezing tolerance and improves the leavening ability after freezing-thaw stress.
  
  
  CONCLUSIONS
  The mutants of the industrial baker's yeast with enhanced freezing tolerance and leavening ability in lean dough were developed by genetic engineering. These strains had excellent potential industrial applications.
authors.affiliation: College of Biological Engineering, Tianjin Agricultural University, Tianjin, 300384, People's Republic of China.|Tianjin Engineering Research Center of Agricultural Products Processing, Tianjin, 300384, People's Republic of China.
authors.forename: Xi
authors.initials: X
authors.lastname: Sun
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Industrial Microbiology Key Laboratory, Tianjin University of Science and Technology, Tianjin, 300457, People's Republic of China.|College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People's Republic of China.
authors.forename: Cui-Ying
authors.initials: CY
authors.lastname: Zhang
authors.affiliation: Diagreat Biotechnologies., Ltd, Beijing, 101111, People's Republic of China.
authors.forename: Ming-Yue
authors.initials: MY
authors.lastname: Wu
authors.affiliation: College of Biological Engineering, Tianjin Agricultural University, Tianjin, 300384, People's Republic of China.|Tianjin Engineering Research Center of Agricultural Products Processing, Tianjin, 300384, People's Republic of China.
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Fan
authors.affiliation: College of Biological Engineering, Tianjin Agricultural University, Tianjin, 300384, People's Republic of China.|Tianjin Engineering Research Center of Agricultural Products Processing, Tianjin, 300384, People's Republic of China.
authors.forename: Shan-Na
authors.initials: SN
authors.lastname: Liu
authors.affiliation: College of Biological Engineering, Tianjin Agricultural University, Tianjin, 300384, People's Republic of China.
authors.forename: Wen-Bi
authors.initials: WB
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Industrial Microbiology Key Laboratory, Tianjin University of Science and Technology, Tianjin, 300457, People's Republic of China. xdg@tust.edu.cn.|College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People's Republic of China. xdg@tust.edu.cn.
authors.forename: Dong-Guang
authors.initials: DG
authors.lastname: Xiao
mesh_terms: D000064:Acclimatization / Q000235:genetics*; D003080:Cold Temperature; D005285:Fermentation / Q000235:genetics*; D005433:Flour / Q000382:microbiology; D005615:Freezing; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D030781:Organisms, Genetically Modified; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D014198:Trehalase / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics; D000520:alpha-Glucosidases / Q000235:genetics*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; C582346:MAL62 protein, S cerevisiae; D000520:alpha-Glucosidases; C000614366:NTH1 protein, S cerevisiae; D014198:Trehalase
keywords: Baker’s yeast; Cell viability; Freezing tolerance; Leavening ability; MAL62; NTH1