title: Aggregation-induced emissive nanoparticles for fluorescence signaling in a low cost paper-based immunoassay.
abstract: Low cost paper based immunoassays are receiving interest due to their fast performance and small amounts of biomolecules needed for developing an immunoassay complex. In this work aggregation-induced emissive (AIE) nanoparticles, obtained from a diastereoisomeric mixture of 1,2-di-(4-hydroxyphenyl)-1,2-diphenylethene (TPEDH) in a one-step top-down method, are characterized through Dynamic Light Scattering (DLS), Scanning Electron Microscopy (SEM), and Zeta potential. By measuring the Zeta potential before and after labeling the nanoparticles with antibodies we demonstrate that the colloidal system is stable in a wide pH-range. The AIE-active nanoparticles are deposited on chitosan and glutaraldehyde modified paper pads overcoming the common aggregation-caused quenching (ACQ) effect. Analyte concentrations from 1000ng and below are applied in a model immunocomplex using Goat anti-Rabbit IgG and Rabbit IgG. In the range of 7.81ng-250ng, linear trends with a high R(2) are observed, which leads to a strong increase of the blue fluorescence from the TPEDH nanoparticles.
authors.affiliation: Department of Chemistry, Biosensors and Bioelectronics Laboratory, The Hong Kong University of Science & Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong, China.
authors.forename: Jan F
authors.initials: JF
authors.lastname: Engels
authors.affiliation: Department of Chemistry, Institute for Advanced Study, Institute of Molecular Functional Materials, Division of Biomedical Engineering, Division of Life Science and State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science & Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong, China.
authors.forename: Jesse
authors.initials: J
authors.lastname: Roose
authors.affiliation: Department of Chemistry, Biosensors and Bioelectronics Laboratory, The Hong Kong University of Science & Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong, China.
authors.forename: Demi Shuang
authors.initials: DS
authors.lastname: Zhai
authors.affiliation: Department of Chemistry, Biosensors and Bioelectronics Laboratory, The Hong Kong University of Science & Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong, China.
authors.forename: Ka Man
authors.initials: KM
authors.lastname: Yip
authors.affiliation: Department of Chemistry, Biosensors and Bioelectronics Laboratory, The Hong Kong University of Science & Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong, China.
authors.forename: Mei Suet
authors.initials: MS
authors.lastname: Lee
authors.affiliation: Department of Chemistry, Institute for Advanced Study, Institute of Molecular Functional Materials, Division of Biomedical Engineering, Division of Life Science and State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science & Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong, China. Electronic address: tangbenz@ust.hk.
authors.forename: Ben Zhong
authors.initials: BZ
authors.lastname: Tang
authors.affiliation: Department of Chemistry, Biosensors and Bioelectronics Laboratory, The Hong Kong University of Science & Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong, China. Electronic address: chrenneb@ust.hk.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Renneberg
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry*; D001559:Benzhydryl Compounds / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry; D055664:Electrochemical Techniques; D005456:Fluorescent Dyes / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D006041:Goats; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis* / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry*; D010209:Paper; D011817:Rabbits; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
chemical_list: D000888:Antibodies, Anti-Idiotypic; D001559:Benzhydryl Compounds; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; C048844:anti-IgG; D048271:Chitosan; D005976:Glutaral
keywords: Aggregated-induced emission; Nanoparticles; Paper-based immunoassays; Tetraphenylethene