title: Spirosoma aerophilum sp. nov., isolated from an air sample.
abstract: A rod-shaped, yellow, Gram-stain-negative, non-flagellated, aerobic bacterium, designated 5516J-17T, was isolated from an air sample collected from Jeju Island, Republic of Korea. It grew in the temperature range of 10-37 °C (optimum 28-30 °C), pH 6.0-11.0 (optimum, pH 7.0) and 0-1 % NaCl (w/v). Phylogenetic trees generated using 16S rRNA gene sequences revealed that strain 5516J-17T belongs to the genus Spirosoma, showing 96.9 % sequence similarity to the most closely related species, Spirosoma linguale DSM 74T. The cellular fatty acids comprised large amounts (>10 % of total fatty acids) of summed feature 3 (C16:1ω7c and/or C16:1ω6c) and C16:1ω5c, and moderate amounts (5-10 % of total fatty acids) of iso-C17:0 3-OH, iso-C15:0 and C16:0. The DNA G+C content was 55.7 mol % and MK-7 was the predominant isoprenoid quinone. Polar lipids were phosphatidylethanolamine, two unknown aminophospholipids, one unknown aminolipid and one unknown lipid. On the basis of this phenotypic and polyphasic taxonomy study, it is suggested that strain 5516J-17T represents a novel species within the genus Spirosoma, with the proposed name Spirosoma aerophilum. The type strain is 5516J-17T (= KACC 17323T = DSM 28388T = JCM 19950T).
authors.affiliation: Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 565-851, Republic of Korea.
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 565-851, Republic of Korea.
authors.forename: Jae-Hyung
authors.initials: JH
authors.lastname: Ahn
authors.affiliation: Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 565-851, Republic of Korea.
authors.forename: Hang-Yeon
authors.initials: HY
authors.lastname: Weon
authors.affiliation: Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 565-851, Republic of Korea.
authors.forename: Seung-Beom
authors.initials: SB
authors.lastname: Hong
authors.affiliation: Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 565-851, Republic of Korea.
authors.forename: Soon-Ja
authors.initials: SJ
authors.lastname: Seok
authors.affiliation: Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 565-851, Republic of Korea.
authors.forename: Jeong-Seon
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 565-851, Republic of Korea.
authors.forename: Soon-Wo
authors.initials: SW
authors.lastname: Kwon
mesh_terms: D000392:Air Movements; D015373:Bacterial Typing Techniques; D001482:Base Composition; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7