title: EXPERIMENTAL PARACOCCIDIOIDOMYCOSIS IN PREGNANT RATS.
abstract: Paracoccidioidomycosis (PCM), caused by the dimorphic fungus Paracoccidioides brasiliensis (Pb), is the most prevalent systemic mycosis in Latin America. There are few reports in the literature about the disease damages during pregnancy and the consequences to the fetuses and breeding. This study evaluated the implications of PCM during pregnancy on offspring and mothers in Wistar rats. Groups of rats were submitted to systemic Pb infection, by intraperitoneal infusion, and mated 30 days after the infection date. Immediately after birth, rats and neonates were sacrificed to obtain organs for standard histological examination, morphometric analysis, fungi recovery by plating (CFU) and dosing of anti-Pb antibodies by ELISA. There were no stillbirths or miscarriages, however, the fetuses from infected pregnant rats had lower body and organ weight but the fertility rate was 100%. The largest number of CFU was recovered from the organ of pregnant rats, the pathological examination revealed more severe infection in the same group, further on the largest number of granulomas and fungal field. It can be concluded that the PCM was more severe in the group of pregnant rats, with implications to the weight of offspring.
authors.affiliation: Departamento de Fisioterapia, Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brasil.
authors.forename: Eduardo Alexandre
authors.initials: EA
authors.lastname: Loth
authors.affiliation: Departamento de Fisioterapia, Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brasil.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Cecatto
authors.affiliation: Departamento de Fisioterapia, Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brasil.
authors.forename: Samia Khalil
authors.initials: SK
authors.lastname: Biazim
authors.affiliation: Departamento de Fisioterapia, Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brasil.
authors.forename: José Henrique Fermino
authors.initials: JH
authors.lastname: Ferreira
authors.affiliation: Departamento de Fisioterapia, Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brasil.
authors.forename: Caroline
authors.initials: C
authors.lastname: Danielli
authors.affiliation: Departamento de Fisioterapia, Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brasil.
authors.forename: Rodrigo Daniel
authors.initials: RD
authors.lastname: Genske
authors.affiliation: Departamento de Farmácia, Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brasil.
authors.forename: Rinaldo Ferreira
authors.initials: RF
authors.lastname: Gandra
authors.affiliation: Departamento de Patologia, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors.forename: Marcello Fabiano de
authors.initials: MF
authors.lastname: Franco
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000908:Antibodies, Fungal / Q000032:analysis*; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015769:Granuloma, Respiratory Tract / Q000382:microbiology / Q000473:pathology; D008107:Liver Diseases / Q000382:microbiology* / Q000473:pathology; D008172:Lung Diseases, Fungal / Q000382:microbiology* / Q000473:pathology; D009929:Organ Size; D010229:Paracoccidioidomycosis / Q000473:pathology; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology* / Q000473:pathology; D017208:Rats, Wistar
chemical_list: D000908:Antibodies, Fungal