title: Pyrosequencing analysis of bacterial communities in Lake Bosten, a large brackish inland lake in the arid northwest of China.
abstract: The bacteria inhabiting brackish lake environments are poorly known, and there are few studies on the microbial diversity of these environments. Lake Bosten, a large brackish inland lake, is the largest lake in Xinjiang Province in northwestern China. Because sediments record past limnic changes, the analysis of sedimentary bacteria in Lake Bosten may help elucidate bacterial responses to environmental change. We employed 454 pyrosequencing to investigate the diversity and bacterial community composition in Lake Bosten. A total of 48 230 high-quality sequence reads with 16 314 operational taxonomic units were successfully obtained from the 4 selected samples, and they were numerically dominated by members of the Deltaproteobacteria (17.1%), Chloroflexi (16.1%), Betaproteobacteria (12.6%), Bacteroidetes (6.6%), and Firmicutes (5.7%) groups, accounting for more than 58.1% of the bacterial sequences. The sediment bacterial communities and diversity were consistently different along the 2 geographic environmental gradients: (i) freshwater-brackish water gradient and (ii) oligotrophic-mesotrophic habitat gradient. Deltaproteobacteria, Chloroflexi, and Betaproteobacteria were amplified throughout all of the sampling sites. More Bacteroidetes and Firmicutes were found near the Kaidu River estuary (site 14). Our investigation showed that Proteobacteria did not display any systematic change along the salinity gradient, and numerous 16S rRNA sequences could not be identified at the genus level. Our data will provide a better understanding of the diversity and distribution of bacteria in arid region brackish lakes.
authors.affiliation: a School of Biology and Food Engineering, Chuzhou University, Chuzhou 239000, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: b State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, People's Republic of China.
authors.forename: Guang
authors.initials: G
authors.lastname: Gao
authors.affiliation: b State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, People's Republic of China.
authors.forename: Xiangming
authors.initials: X
authors.lastname: Tang
authors.affiliation: b State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, People's Republic of China.
authors.forename: Keqiang
authors.initials: K
authors.lastname: Shao
authors.affiliation: b State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Gong
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics*; D041963:Bacteroidetes / Q000145:classification / Q000235:genetics; D020562:Betaproteobacteria / Q000145:classification / Q000235:genetics; D044822:Biodiversity; D002681:China; D041862:Chloroflexi / Q000145:classification / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D020564:Deltaproteobacteria / Q000145:classification / Q000235:genetics; D017753:Ecosystem; D000068536:Bacillota / Q000145:classification / Q000235:genetics; D019015:Geologic Sediments / Q000382:microbiology; D060106:Lakes / Q000382:microbiology*; D010802:Phylogeny; D020560:Proteobacteria / Q000145:classification / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: 454 pyrosequencing; brackish lake; communauté bactérienne de sédiments; eutrophication; eutrophisation; lac saumâtre; pyroséquençage 454; salinization; sediment bacterial community