title: Phylogenetic analysis of β-xylanase SRXL1 of Sporisorium reilianum and its relationship with families (GH10 and GH11) of Ascomycetes and Basidiomycetes.
abstract: In this paper, the amino acid sequence of the β-xylanase SRXL1 of Sporisorium reilianum, which is a pathogenic fungus of maize was used as a model protein to find its phylogenetic relationship with other xylanases of Ascomycetes and Basidiomycetes and the information obtained allowed to establish a hypothesis of monophyly and of biological role. 84 amino acid sequences of β-xylanase obtained from the GenBank database was used. Groupings analysis of higher-level in the Pfam database allowed to determine that the proteins under study were classified into the GH10 and GH11 families, based on the regions of highly conserved amino acids, 233-318 and 180-193 respectively, where glutamate residues are responsible for the catalysis.
authors.affiliation: Universidad Politécnica de Pachuca. Zempoala, Hidalgo, México.
authors.forename: Jorge
authors.initials: J
authors.lastname: Álvarez-Cervantes
authors.affiliation: Laboratory of Biotechnology, Research Center for Biological Sciences, Universidad Autónoma de Tlaxcala, Tlaxcala, México.
authors.forename: Gerardo
authors.initials: G
authors.lastname: Díaz-Godínez
authors.affiliation: Universidad Politécnica de Pachuca. Zempoala, Hidalgo, México.
authors.forename: Yuridia
authors.initials: Y
authors.lastname: Mercado-Flores
authors.affiliation: Molecular Glycobiotechnology Group, Discipline of Biochemistry, National University of Ireland Galway, Galway, Ireland.
authors.forename: Vijai Kumar
authors.initials: VK
authors.lastname: Gupta
authors.affiliation: Universidad Politécnica de Pachuca. Zempoala, Hidalgo, México.
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Anducho-Reyes
mesh_terms: D001203:Ascomycota / Q000201:enzymology* / Q000235:genetics; D001487:Basidiomycota / Q000201:enzymology* / Q000235:genetics; D017124:Conserved Sequence; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry / Q000235:genetics*; D019143:Evolution, Molecular; D005656:Fungal Proteins / Q000235:genetics; D008958:Models, Molecular; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D020539:Sequence Analysis, Protein; D013329:Structure-Activity Relationship
chemical_list: D005656:Fungal Proteins; D043364:Endo-1,4-beta Xylanases