title: Graphene Oxide Nanosheets Reshape Synaptic Function in Cultured Brain Networks.
abstract: Graphene offers promising advantages for biomedical applications. However, adoption of graphene technology in biomedicine also poses important challenges in terms of understanding cell responses, cellular uptake, or the intracellular fate of soluble graphene derivatives. In the biological microenvironment, graphene nanosheets might interact with exposed cellular and subcellular structures, resulting in unexpected regulation of sophisticated biological signaling. More broadly, biomedical devices based on the design of these 2D planar nanostructures for interventions in the central nervous system require an accurate understanding of their interactions with the neuronal milieu. Here, we describe the ability of graphene oxide nanosheets to down-regulate neuronal signaling without affecting cell viability.
authors.affiliation: Life Science Department, University of Trieste , 34127 Trieste, Italy.
authors.forename: Rossana
authors.initials: R
authors.lastname: Rauti
authors.affiliation: Nanomedicine Lab, School of Medicine and National Graphene Institute, Faculty of Medical & Human Sciences, University of Manchester , M13 9PL Manchester, United Kingdom.
authors.forename: Neus
authors.initials: N
authors.lastname: Lozano
authors.affiliation: Departamento de Química Orgánica, Facultad de Ciencias y Tecnologías Químicas-IRICA, Universidad de Castilla La Mancha , 13071 Ciudad Real, Spain.
authors.forename: Veronica
authors.initials: V
authors.lastname: León
authors.affiliation: Life Science Department, University of Trieste , 34127 Trieste, Italy.|ELETTRA Synchrotron Light Source , 34149 Trieste, Italy.
authors.forename: Denis
authors.initials: D
authors.lastname: Scaini
authors.affiliation: International School for Advanced Studies (SISSA) , 34136 Trieste, Italy.
authors.forename: Mattia
authors.initials: M
authors.lastname: Musto
authors.affiliation: ELETTRA Synchrotron Light Source , 34149 Trieste, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Rago
authors.affiliation: International School for Advanced Studies (SISSA) , 34136 Trieste, Italy.
authors.forename: Francesco P
authors.initials: FP
authors.lastname: Ulloa Severino
authors.affiliation: Department of Chemical and Pharmaceutical Sciences, University of Trieste , 34127 Trieste, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Fabbro
authors.affiliation: ELETTRA Synchrotron Light Source , 34149 Trieste, Italy.
authors.forename: Loredana
authors.initials: L
authors.lastname: Casalis
authors.affiliation: Departamento de Química Orgánica, Facultad de Ciencias y Tecnologías Químicas-IRICA, Universidad de Castilla La Mancha , 13071 Ciudad Real, Spain.
authors.forename: Ester
authors.initials: E
authors.lastname: Vázquez
authors.affiliation: Nanomedicine Lab, School of Medicine and National Graphene Institute, Faculty of Medical & Human Sciences, University of Manchester , M13 9PL Manchester, United Kingdom.
authors.forename: Kostas
authors.initials: K
authors.lastname: Kostarelos
authors.affiliation: Department of Chemical and Pharmaceutical Sciences, University of Trieste , 34127 Trieste, Italy.|CIC BiomaGUNE, Parque Tecnológico de San Sebastián, Paseo Miramón, 182, 20009 San Sebastián, Guipúzcoa, Spain.|Basque Foundation for Science , Ikerbasque, Bilbao 48013, Spain.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Prato
authors.affiliation: Life Science Department, University of Trieste , 34127 Trieste, Italy.|International School for Advanced Studies (SISSA) , 34136 Trieste, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Ballerini
mesh_terms: D000818:Animals; D001921:Brain / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D018929:Cell Culture Techniques; D015536:Down-Regulation; D005455:Fluorescent Antibody Technique; D006108:Graphite / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D009415:Nerve Net / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D061848:Optical Imaging; D010087:Oxides / Q000737:chemistry*; D010316:Particle Size; D051381:Rats; D013499:Surface Properties; D013569:Synapses / Q000502:physiology
chemical_list: D010087:Oxides; D006108:Graphite; D002118:Calcium
keywords: FMI-43; exocytosis; graphene; microvesicles; nanotechnology; patch-clamp; synaptic terminals