title: Quantitative proteomic analysis of mice corneal tissues reveals angiogenesis-related proteins involved in corneal neovascularization.
abstract: Corneal neovascularization (CNV) was induced in Balb/c mice by alkali burns in the central area of the cornea with a diameter of 2.5mm. After fourteen days, the cornea from one eye was collected for histological staining for CNV examination, while the cornea from the other eye of the same mouse was harvested for proteomic analysis. The label-free quantitative proteomic approach was applied to analyze five normal corneal tissues (normal group mice n=5) and five corresponding neovascularized corneal tissues (model group mice n=5). A total of 2124 proteins were identified, and 1682 proteins were quantified from these corneal tissues. Among these quantified proteins, 290 proteins were significantly changed between normal and alkali burned corneal tissues. Of these significantly changed proteins, 35 were reported or predicted as angiogenesis-related proteins. Then, these 35 proteins were analyzed using Ingenuity Pathway Analysis Software, resulting in 26 proteins enriched and connected to each other in the protein-protein interaction network, such as Lcn-2, αB-crystallin and Serpinf1 (PEDF). These three significantly changed proteins were selected for further Western blotting validation. Consistent with the quantitative proteomic results, Western blotting showed that Lcn-2 and αB-crystallin were significantly up-regulated in CNV model, while PEDF was down-regulated. This study provided increased understanding of angiogenesis-related proteins involved in corneal vascular development, which will be useful in the ophthalmic clinic of specifically target angiogenesis.
authors.affiliation: Department of Ophthalmology, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Minqian
authors.initials: M
authors.lastname: Shen
authors.affiliation: Department of Analytical Chemistry and CAS Key Laboratory of Receptor Research, Shanghai Institute of MateriaMedica, Chinese Academy of Sciences, Shanghai 201203, China; SIMMUOMICS Laboratory, Joint Research Laboratory of Translational "OMICS" between Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China and University of Ottawa, Canada.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Tao
authors.affiliation: Department of Ophthalmology, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Department of Analytical Chemistry and CAS Key Laboratory of Receptor Research, Shanghai Institute of MateriaMedica, Chinese Academy of Sciences, Shanghai 201203, China; SIMMUOMICS Laboratory, Joint Research Laboratory of Translational "OMICS" between Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China and University of Ottawa, Canada.
authors.forename: Xing
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Ophthalmology, Zhongshan Hospital, Fudan University, Shanghai 200032, China. Electronic address: yuanfei20@yahoo.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Yuan
authors.affiliation: Department of Analytical Chemistry and CAS Key Laboratory of Receptor Research, Shanghai Institute of MateriaMedica, Chinese Academy of Sciences, Shanghai 201203, China; SIMMUOMICS Laboratory, Joint Research Laboratory of Translational "OMICS" between Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China and University of Ottawa, Canada. Electronic address: zhouhu@simm.ac.cn.
authors.forename: Hu
authors.initials: H
authors.lastname: Zhou
mesh_terms: D000209:Acute-Phase Proteins / Q000032:analysis; D000818:Animals; D015153:Blotting, Western; D003315:Cornea / Q000737:chemistry*; D016510:Corneal Neovascularization / Q000209:etiology*; D003459:Crystallins / Q000032:analysis; D005136:Eye Proteins / Q000032:analysis; D000071068:Lipocalin-2; D054834:Lipocalins / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D009414:Nerve Growth Factors / Q000032:analysis; D015513:Oncogene Proteins / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D015843:Serpins / Q000032:analysis; D000099174:Pigment Epithelium-Derived Factor
chemical_list: D000209:Acute-Phase Proteins; D003459:Crystallins; D005136:Eye Proteins; D000071068:Lipocalin-2; D054834:Lipocalins; D009414:Nerve Growth Factors; D015513:Oncogene Proteins; D015843:Serpins; D000099174:Pigment Epithelium-Derived Factor; C071332:Lcn2 protein, mouse
keywords: Alkali burn; Corneal neovascularization; Mice; Proteomics