title: [Expression of heme oxygenase-1 in nasal polyps and regulation by glucocorticoid].
abstract: OBJECTIVE
  To evaluate the expression and possible modulation of heme oxygenase-1 (HO-1) in nasal polyps of patients with chronic rhinosinusitis with nasal polyps (CRSwNP).
  
  
  METHODS
  Nasal polyps and uncinate process tissues were collected from 25 CRSwNP patients and 19 healthy controls with nasal septal deviation. HO-1 expression was examined using qRT-PCR, immunohistochemistric staining and Western blot analysis. Moreover, additional uncinate process mucosal samples of 15 healthy controls with nasal septal deviation were harvested for nasal explant culture experiments. HO-1 expression was measured in cultured nasal explant in response to specific inflammatory and glucocorticoid stimulation. SPSS 20.0 software was used to analyze the data.
  
  
  RESULTS
  The mRNA and protein expression of HO-1 was significantly increased in polyp tissues, 1.220±0.397 in mRNA and 1.409±0.701 in protein, compared with healthy controls 0.464±0.318 in mRNA and 0.017±0.1147 in protein (U=22.00 in mRNA and U=1.00 in protein, both P< 0.05). The immunohistochemical results showed that HO-1 was mainly distributed in the epithelial layer, submucosal glands and inflammatory cells in nasal tissues. Nasal explant culture experiments demonstrated that HO-1 mRNA was upregulated by IL-17A. The HO-1 mRNA level before the stimulation was 1.000, and 17.264±4.275 after the stimulation of 1 ng/ml IL-17A (U=0, P<0.05), 19.128±4.605 after the stimulation of 10 ng/ml IL-17A (U=0, P<0.05), but was significantly suppressed after stimulation with glucocorticoids (dexamethasone, DEX). The mRNA level after the glucocorticoids stimulation was 0.370±0.101 (U=0, P<0.05) and 0.316±0.167 (U=0, P<0.05) respectively. Furthermore, the HO-1 mRNA was inhibited by TGF-β1, the mRNA level was 0.217±0.322 (U=0, P<0.05), 0.070±0.070 (U=0, P<0.05), respectively.
  
  
  CONCLUSIONS
  Increased HO-1 expression may play a role in the pathogenesis of CRSwNP, which may be considered as the therapeutic target.
authors.affiliation: Otorhinolaryngology Hospital, the First Affiliated Hospital of Sun Yatsen University, Guangzhou 510080, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Otorhinolaryngology Hospital, the First Affiliated Hospital of Sun Yatsen University, Guangzhou 510080, China.
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Cheng
authors.affiliation: Otorhinolaryngology Hospital, the First Affiliated Hospital of Sun Yatsen University, Guangzhou 510080, China.
authors.forename: Kejun
authors.initials: K
authors.lastname: Zuo
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Guozhen
authors.initials: G
authors.lastname: Meng
authors.affiliation: Otorhinolaryngology Hospital, the First Affiliated Hospital of Sun Yatsen University, Guangzhou 510080, China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Wen
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Huabin
authors.initials: H
authors.lastname: Li
mesh_terms: D015153:Blotting, Western; D016022:Case-Control Studies; D002908:Chronic Disease; D003907:Dexamethasone / Q000494:pharmacology; D005938:Glucocorticoids / Q000494:pharmacology*; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D006801:Humans; D020381:Interleukin-17 / Q000494:pharmacology; D009298:Nasal Polyps / Q000150:complications / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012220:Rhinitis / Q000150:complications / Q000378:metabolism*; D012852:Sinusitis / Q000150:complications / Q000378:metabolism*; D046509:Tissue Culture Techniques; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D005938:Glucocorticoids; C508717:IL17A protein, human; D020381:Interleukin-17; D012333:RNA, Messenger; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; D003907:Dexamethasone; C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1