title: Laxitextines A and B, Cyathane Xylosides from the Tropical Fungus Laxitextum incrustatum.
abstract: Bioassay-guided fractionation of the mycelial extract of a basidiomycete culture collected in Kenya led to the isolation of two new cyathane diterpenoids named laxitextines A (1) and B (2). The producer strain was characterized by detailed taxonomic studies based on rDNA using the 5.8S gene region, the internal transcribed spacer 2 (ITS2), and part of the large subunit that identified the fungus as Laxitextum incrustatum. The structures of 1 and 2 were elucidated by NMR spectroscopic and mass spectrometric analyses. Both compounds exhibited moderate activities against Gram-positive bacteria Bacillus subtilis (DSM 10), Staphylococcus aureus (DSM 346), and methicillin-resistant Staph. aureus (DSM 1182). The two compounds also showed variable antiproliferative activities against mouse fibroblast (L929) and selected human cell lines (breast cancer MCF-7, epidermoid carcinoma A431, and umbilical vein endothelial HUVEC). The IC50 values with respect to the MCF-7 cell line for compounds 1 and 2 were 2.3 and 2.0 μM, respectively.
authors.affiliation: Institut für Chemie, Technische Universität Berlin , Strasse des 17. Juni 124, 10623 Berlin, Germany.
authors.forename: Cynthia M
authors.initials: CM
authors.lastname: Mudalungu
authors.affiliation: Department of Microbial Drugs, Braunschweig, Helmholtz Centre for Infection Research GmbH , Inhoffenstraße 7, 38124 Braunschweig, Germany.|German Centre for Infection Research, partner site Hannover-Braunschweig , Inhoffenstraße 7, 38124 Braunschweig, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Richter
authors.affiliation: Department of Microbial Drugs, Braunschweig, Helmholtz Centre for Infection Research GmbH , Inhoffenstraße 7, 38124 Braunschweig, Germany.|German Centre for Infection Research, partner site Hannover-Braunschweig , Inhoffenstraße 7, 38124 Braunschweig, Germany.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Wittstein
authors.affiliation: Institut für Chemie, Technische Universität Berlin , Strasse des 17. Juni 124, 10623 Berlin, Germany.
authors.forename: Muna Ali
authors.initials: MA
authors.lastname: Abdalla
authors.affiliation: Department of Chemistry, Egerton University , P.O. Box 536, Egerton, Kenya.
authors.forename: Josphat C
authors.initials: JC
authors.lastname: Matasyoh
authors.affiliation: Department of Microbial Drugs, Braunschweig, Helmholtz Centre for Infection Research GmbH , Inhoffenstraße 7, 38124 Braunschweig, Germany.|German Centre for Infection Research, partner site Hannover-Braunschweig , Inhoffenstraße 7, 38124 Braunschweig, Germany.
authors.forename: Marc
authors.initials: M
authors.lastname: Stadler
authors.affiliation: Institut für Chemie, Technische Universität Berlin , Strasse des 17. Juni 124, 10623 Berlin, Germany.
authors.forename: Roderich D
authors.initials: RD
authors.lastname: Süssmuth
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D061986:MCF-7 Cells; D016106:Methicillin Resistance / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D004224:Diterpenes; D006027:Glycosides; C497675:cyathane; C000610788:laxitextine A; C000610789:laxitextine B; C010807:xylosides