title: Activation of epithelial proliferation induced by Eimeria acervulina infection in the duodenum may be associated with cholesterol metabolism.
abstract: Cell proliferation in the intestine is commonly occurred during infection and inflammation to replace damaged enterocytes, and cholesterol as an essential constituent of cell membrane, is required for cell proliferation and growth. Here we found that coccidium-challenged (CC) chickens showed severe damages in intestinal structure, a significant increase of cell proliferation, and an activation of genes expression involved in the innate immune response. Compared to control (CON), CC chickens showed a marked decrease of cholesterol (Tch) level in the circulating system, but a significant increase in local duodenum epithelium. Increase of LDLR protein combined with a significant decrease of CYP27A1 protein expression in duodenum epithelium may contribute to intestinal cholesterol accumulation in CC chickens. Moreover, we found miRNAs targeting to CYP27A1 gene participating in post-transcriptional regulation. Hence, these results provide a new insight for the intervention of epithelial proliferation and cholesterol metabolism in the gastrointestinal tracts.
authors.affiliation: Key Laboratory of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Sun
authors.affiliation: Key Laboratory of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Dong
authors.affiliation: Department of Veterinary Parasitic Disease, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
authors.forename: Zhenchao
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Laboratory of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Key Laboratory of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China.
authors.forename: Yingdong
authors.initials: Y
authors.lastname: Ni
authors.affiliation: Department of Functional Genomics and Bioregulation, Institute of Animal Genetics, FLI, Mariensee, Neustadt a Rbg, Germany.
authors.forename: Roland
authors.initials: R
authors.lastname: Grossmann
authors.affiliation: Key Laboratory of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China.
authors.forename: Ruqian
authors.initials: R
authors.lastname: Zhao
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000502:physiology; D002645:Chickens; D002784:Cholesterol / Q000097:blood / Q000378:metabolism*; D003048:Coccidiosis / Q000097:blood / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D000072501:Cytochrome P450 Family 27 / Q000378:metabolism; D004378:Duodenal Diseases / Q000097:blood / Q000378:metabolism / Q000469:parasitology / Q000662:veterinary*; D004386:Duodenum / Q000378:metabolism / Q000469:parasitology / Q000473:pathology; D004539:Eimeria / Q000502:physiology*; D004848:Epithelium / Q000378:metabolism / Q000469:parasitology / Q000473:pathology; D006367:HeLa Cells; D006801:Humans; D011201:Poultry Diseases / Q000097:blood / Q000378:metabolism* / Q000469:parasitology* / Q000473:pathology
chemical_list: D002784:Cholesterol; D000072501:Cytochrome P450 Family 27
keywords: cell proliferation; cholesterol; coccidial infection; miRNAs; small intestine