title: Synthesis of polyester urethane urea and fabrication of elastomeric nanofibrous scaffolds for myocardial regeneration.
abstract: Fabrication of bioactive scaffolds is one of the most promising strategies to reconstruct the infarcted myocardium. In this study, we synthesized polyester urethane urea (PEUU), further blended it with gelatin and fabricated PEUU/G nanofibrous scaffolds. Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), differential scanning calorimetry (DSC) and X-ray diffraction were used for the characterization of the synthesized PEUU and properties of nanofibrous scaffolds were evaluated using scanning electron microscopy (SEM), ATR-FTIR, contact angle measurement, biodegradation test, tensile strength analysis and dynamic mechanical analysis (DMA). In vitro biocompatibility studies were performed using cardiomyocytes. DMA analysis showed that the scaffolds could be reshaped with cyclic deformations and might remain stable in the frequencies of the physiological activity of the heart. On the whole, our study suggests that aligned PEUU/G 70:30 nanofibrous scaffolds meet the required specifications for cardiac tissue engineering and could be used as a promising construct for myocardial regeneration.
authors.affiliation: Department of Textile engineering, Isfahan university of technology, Isfahan 84156-83111, Iran.
authors.forename: Elham Sadat
authors.initials: ES
authors.lastname: Jamadi
authors.affiliation: Department of Textile engineering, Isfahan university of technology, Isfahan 84156-83111, Iran.
authors.forename: Laleh
authors.initials: L
authors.lastname: Ghasemi-Mobarakeh
authors.affiliation: Department of Textile engineering, Isfahan university of technology, Isfahan 84156-83111, Iran. Electronic address: morshed@cc.iut.ac.ir.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Morshed
authors.affiliation: Department of Chemical Engineering, Isfahan university of technology, Isfahan 84156-83111, Iran.
authors.forename: Morteza
authors.initials: M
authors.lastname: Sadeghi
authors.affiliation: Department of Mechanical Engineering, Faculty of Engineering, 2 Engineering Drive 3, National University of Singapore, Singapore 117576, Singapore. Electronic address: nanotechmpp@gmail.com.
authors.forename: Molamma P
authors.initials: MP
authors.lastname: Prabhakaran
authors.affiliation: Department of Mechanical Engineering, Faculty of Engineering, 2 Engineering Drive 3, National University of Singapore, Singapore 117576, Singapore.
authors.forename: Seeram
authors.initials: S
authors.lastname: Ramakrishna
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D002152:Calorimetry, Differential Scanning; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005780:Gelatin / Q000737:chemistry; D006321:Heart / Q000502:physiology; D008855:Microscopy, Electron, Scanning; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism; D057139:Nanofibers / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry*; D011817:Rabbits; D051381:Rats; D012038:Regeneration / Q000502:physiology; D017550:Spectroscopy, Fourier Transform Infrared; D013718:Tensile Strength; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D011140:Polyurethanes; C115016:polyetherurethane; D005780:Gelatin
keywords: Electrospinning; Myocardial regeneration; Polyester urethane; Tissue engineering