title: Uncovering the diversity in the Amazophrynella minuta complex: integrative taxonomy reveals a new species of Amazophrynella (Anura, Bufonidae) from southern Peru.
abstract: A new species of the genus Amazophrynella (Anura, Bufonidae) is described from the departments of Madre de Dios, Cusco and Junin in Peru. An integrative taxonomy approach is used. A morphological diagnosis, morphometrics comparisons, description of the advertisement call, and the phylogenetic relationships of the new species are provided. Amazophrynella javierbustamantei sp. n. differs from other species of Amazophrynella by: intermediate body-size (snout-vent length 14.9 mm in males, n = 26 and 19.6 mm in females, n = 20), tuberculate skin texture of body, greatest hand length of the Amazophrynella spp. (3.6 mm in males, n = 26 and 4.6 mm in females, n = 20), venter coloration yellowish, tiny rounded black points covering the venter, and thirteen molecular autapomorphies in the 16S RNA gene. Its distribution varies from 215 to 708 m a.s.l. This discovery highlights the importance of the remnant forest in preserving the biodiversity in Peru, and increase in seven the species formally described in the genus Amazophrynella.
authors.affiliation: Programa de Pós-graduação em Genética Conservação e Biologia Evolutiva- Instituto Nacional de Pesquisas da Amazônia-INPA, Av. André Araújo, 2936, Manaus, Brazil; Laboratório de Genética e Evolução Animal, Departamento de Genética, ICB, Universidade Federal do Amazonas, Av. Gen. Rodrigo Octávio Jordão Ramos, 3000, Manaus, Brazil.
authors.forename: Rommel R
authors.initials: RR
authors.lastname: Rojas
authors.affiliation: Museo de Historia Natural, Universidad Nacional de San Antonio Abad del Cusco, Peru.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Chaparro
authors.affiliation: Laboratório de Genética e Evolução Animal, Departamento de Genética, ICB, Universidade Federal do Amazonas, Av. Gen. Rodrigo Octávio Jordão Ramos, 3000, Manaus, Brazil; Programa de Pós-Graduação em Biodiversidade e Biotecnologia, Av. Gen. Rodrigo Octávio Jordão Ramos, 3000, Manaus, Brazil.
authors.forename: Vinícius Tadeu De
authors.initials: VT
authors.lastname: Carvalho
authors.affiliation: Universidade Regional do Cariri, Centro de Ciências Biológicas e da Saúde, Departamento de Ciências Biológicas, Campus do Pimenta, Rua Cel. Antônio Luiz, 1161, Bairro do Pimenta, Crato, Brazil.
authors.forename: Robson W
authors.initials: RW
authors.lastname: Ávila
authors.affiliation: Laboratório de Genética e Evolução Animal, Departamento de Genética, ICB, Universidade Federal do Amazonas, Av. Gen. Rodrigo Octávio Jordão Ramos, 3000, Manaus, Brazil.
authors.forename: Izeni Pires
authors.initials: IP
authors.lastname: Farias
authors.affiliation: Laboratório de Genética e Evolução Animal, Departamento de Genética, ICB, Universidade Federal do Amazonas, Av. Gen. Rodrigo Octávio Jordão Ramos, 3000, Manaus, Brazil.
authors.forename: Tomas
authors.initials: T
authors.lastname: Hrbek
authors.affiliation: Departamento de Biologia, ICB, Universidade Federal do Amazonas, Av. Gen. Rodrigo Octávio Jordão Ramos, 3000, Manaus, Brazil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Gordo
keywords: Amphibian; Southern Peru; Tree Toad; conservation; integrative taxonomy