title: CORRELATED EVOLUTION OF GENOME SIZE AND CELL VOLUME IN DIATOMS (BACILLARIOPHYCEAE)(1).
abstract: A correlation between genome size and cell volume has been observed across diverse assemblages of eukaryotes. We examined this relationship in diatoms (Bacillariophyceae), a phylum in which cell volume is of critical ecological and biogeochemical importance. In addition to testing whether there is a predictive relationship across extant species, we tested whether evolutionary divergences in genome size were correlated with evolutionary divergences in cell size (using independent contrasts). We estimated total DNA content for 16 diatom species using a flow cytometer and estimated cell volumes using critical dimensions with scaling equations. Our independent contrast analyses indicated a significant correlated evolution between genome size and cell volume. We then explored the evolutionary and ecological implications of this evolutionary relationship. Diatom cell volume is an important component of the global carbon cycle; therefore, understanding the mechanisms that drive diatom genome evolution has both evolutionary and ecological importance.
authors.affiliation: Center for Coastal Marine Sciences, Biological Sciences Department, California Polytechnic State University, San Luis Obispo, California 93407, USAInstitute of Marine and Coastal Sciences, Rutgers University, New Brunswick, New Jersey 08901, USABiological Sciences Department, California Polytechnic State University, San Luis Obispo, California 93407, USACenter for Coastal Marine Sciences, Biological Sciences Department, California Polytechnic State University, San Luis Obispo, California 93407, USA.
authors.forename: Jessica A
authors.initials: JA
authors.lastname: Connolly
authors.affiliation: Center for Coastal Marine Sciences, Biological Sciences Department, California Polytechnic State University, San Luis Obispo, California 93407, USAInstitute of Marine and Coastal Sciences, Rutgers University, New Brunswick, New Jersey 08901, USABiological Sciences Department, California Polytechnic State University, San Luis Obispo, California 93407, USACenter for Coastal Marine Sciences, Biological Sciences Department, California Polytechnic State University, San Luis Obispo, California 93407, USA.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Oliver
authors.affiliation: Center for Coastal Marine Sciences, Biological Sciences Department, California Polytechnic State University, San Luis Obispo, California 93407, USAInstitute of Marine and Coastal Sciences, Rutgers University, New Brunswick, New Jersey 08901, USABiological Sciences Department, California Polytechnic State University, San Luis Obispo, California 93407, USACenter for Coastal Marine Sciences, Biological Sciences Department, California Polytechnic State University, San Luis Obispo, California 93407, USA.
authors.forename: Jeremy M
authors.initials: JM
authors.lastname: Beaulieu
authors.affiliation: Center for Coastal Marine Sciences, Biological Sciences Department, California Polytechnic State University, San Luis Obispo, California 93407, USAInstitute of Marine and Coastal Sciences, Rutgers University, New Brunswick, New Jersey 08901, USABiological Sciences Department, California Polytechnic State University, San Luis Obispo, California 93407, USACenter for Coastal Marine Sciences, Biological Sciences Department, California Polytechnic State University, San Luis Obispo, California 93407, USA.
authors.forename: Charles A
authors.initials: CA
authors.lastname: Knight
authors.affiliation: Center for Coastal Marine Sciences, Biological Sciences Department, California Polytechnic State University, San Luis Obispo, California 93407, USAInstitute of Marine and Coastal Sciences, Rutgers University, New Brunswick, New Jersey 08901, USABiological Sciences Department, California Polytechnic State University, San Luis Obispo, California 93407, USACenter for Coastal Marine Sciences, Biological Sciences Department, California Polytechnic State University, San Luis Obispo, California 93407, USA.
authors.forename: Lars
authors.initials: L
authors.lastname: Tomanek
authors.affiliation: Center for Coastal Marine Sciences, Biological Sciences Department, California Polytechnic State University, San Luis Obispo, California 93407, USAInstitute of Marine and Coastal Sciences, Rutgers University, New Brunswick, New Jersey 08901, USABiological Sciences Department, California Polytechnic State University, San Luis Obispo, California 93407, USACenter for Coastal Marine Sciences, Biological Sciences Department, California Polytechnic State University, San Luis Obispo, California 93407, USA.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Moline
keywords: Bacillariophyceae; cellular volume; comparative methods; correlated evolution; diatom evolution; genome size variation