title: The Probiotic Lactobacillus Prevents Citrobacter rodentium-Induced Murine Colitis in a TLR2-Dependent Manner.
abstract: The main objective of this study was to investigate whether Lactobacillus rhamnosus GG (LGG) ameliorated the effects of Citrobactor rodentium infection in Toll-like receptor 2 (TLR2) knockout (KO) and TLR4 KO mice, as well as in wild-type C57BL/6 (B6) mice. TLR2 KO, TLR4 KO, and B6 mice were divided into three groups per each strain. Each group had an uninfected control group (n = 5), C. rodentium-infected group (n = 8), and LGG-pretreated C. rodentium-infected group (n = 8). The survival rate of B6 mice infected with C. rodentium was higher when pretreated with LGG. Pretreatment with LGG ameliorated C. rodentium-induced mucosal hyperplasia in B6 and TLR4 KO mice. However, in C-rodentium-infected TLR2 KO mice, mucosal hyperplasia persisted, regardless of pretreatment with LGG. In addition, LGG-pretreated B6 and TLR4 KO mice showed a decrease in spleen weight and downregulation of tumor necrosis factor alpha, interferon gamma, and monocyte chemotactic protein 1 mRNA expression compared with the non-pretreated group. In contrast, such changes were not observed in TLR2 KO mice, regardless of pretreatment with LGG. From the above results, we conclude that pretreatment with LGG ameliorates C. rodentium-induced colitis in B6 and TLR4 KO mice, but not in TLR2 KO mice. Therefore, LGG protects mice from C. rodentium-induced colitis in a TLR2-dependent manner.
authors.affiliation: Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Seung-Hyun
authors.initials: SH
authors.lastname: Ryu
authors.affiliation: Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Jong-Hyung
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Soo-Young
authors.initials: SY
authors.lastname: Choi
authors.affiliation: Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Hee-Yeon
authors.initials: HY
authors.lastname: Jeon
authors.affiliation: Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Jin-Il
authors.initials: JI
authors.lastname: Park
authors.affiliation: Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Jun-Young
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Seung-Hoon
authors.initials: SH
authors.lastname: Ham
authors.affiliation: Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Yang-Kyu
authors.initials: YK
authors.lastname: Choi
mesh_terms: D000818:Animals; D044082:Citrobacter rodentium; D003092:Colitis / Q000378:metabolism* / Q000382:microbiology* / Q000401:mortality / Q000473:pathology; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D004756:Enterobacteriaceae Infections / Q000378:metabolism* / Q000382:microbiology* / Q000401:mortality / Q000473:pathology; D005260:Female; D015870:Gene Expression; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D007778:Lactobacillus / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D019936:Probiotics / Q000008:administration & dosage*; D012333:RNA, Messenger / Q000235:genetics; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism*; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4
keywords: Citrobacter rodentium; Lactobacillus rhamnosus; Toll-like receptor; colitis; probiotic