title: Transplantation of Defined Populations of Differentiated Human Neural Stem Cell Progeny.
abstract: Many neurological injuries are likely too extensive for the limited repair capacity of endogenous neural stem cells (NSCs). An alternative is to isolate NSCs from a donor, and expand them in vitro as transplantation material. Numerous groups have already transplanted neural stem and precursor cells. A caveat to this approach is the undefined phenotypic distribution of the donor cells, which has three principle drawbacks: (1) Stem-like cells retain the capacity to proliferate in vivo. (2) There is little control over the cells' terminal differentiation, e.g., a graft intended to replace neurons might choose a predominantly glial fate. (3) There is limited ability of researchers to alter the combination of cell types in pursuit of a precise treatment. We demonstrate a procedure for differentiating human neural precursor cells (hNPCs) in vitro, followed by isolation of the neuronal progeny. We transplanted undifferentiated hNPCs or a defined concentration of hNPC-derived neurons into mice, then compared these two groups with regard to their survival, proliferation and phenotypic fate. We present evidence suggesting that in vitro-differentiated-and-purified neurons survive as well in vivo as their undifferentiated progenitors, and undergo less proliferation and less astrocytic differentiation. We also describe techniques for optimizing low-temperature cell preservation and portability.
authors.affiliation: Department of Neurosurgery, McKnight Brain Institute, University of Florida, Gainesville, FL 32610-0261, USA.
authors.forename: Jeff M
authors.initials: JM
authors.lastname: Fortin
authors.affiliation: Department of Neurosurgery, McKnight Brain Institute, University of Florida, Gainesville, FL 32610-0261, USA.|Neural Stem Cell and Regenerative Neuroscience Laboratory, Department of Anatomical Sciences &Shiraz Stem Cell Institute, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Hassan
authors.initials: H
authors.lastname: Azari
authors.affiliation: Department of Neurosurgery, McKnight Brain Institute, University of Florida, Gainesville, FL 32610-0261, USA.
authors.forename: Tong
authors.initials: T
authors.lastname: Zheng
authors.affiliation: Department of Neurosurgery, McKnight Brain Institute, University of Florida, Gainesville, FL 32610-0261, USA.
authors.forename: Roya P
authors.initials: RP
authors.lastname: Darioosh
authors.affiliation: Department of Neurosurgery, McKnight Brain Institute, University of Florida, Gainesville, FL 32610-0261, USA.
authors.forename: Michael E
authors.initials: ME
authors.lastname: Schmoll
authors.affiliation: Department of Neurosurgery, McKnight Brain Institute, University of Florida, Gainesville, FL 32610-0261, USA.
authors.forename: Vinata
authors.initials: V
authors.lastname: Vedam-Mai
authors.affiliation: Department of Neurosurgery, McKnight Brain Institute, University of Florida, Gainesville, FL 32610-0261, USA.
authors.forename: Loic P
authors.initials: LP
authors.lastname: Deleyrolle
authors.affiliation: Department of Neurosurgery, McKnight Brain Institute, University of Florida, Gainesville, FL 32610-0261, USA.
authors.forename: Brent A
authors.initials: BA
authors.lastname: Reynolds
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D058948:Cell Tracking / Q000379:methods; D015925:Cryopreservation / Q000379:methods*; D015870:Gene Expression; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods; D007276:Injections, Intraventricular; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D058953:Neural Stem Cells / Q000166:cytology* / Q000502:physiology; D055495:Neurogenesis / Q000502:physiology*; D009457:Neuroglia / Q000166:cytology / Q000502:physiology; D009474:Neurons / Q000166:cytology* / Q000502:physiology / Q000637:transplantation*; D010641:Phenotype; D033581:Stem Cell Transplantation; D013238:Stereotaxic Techniques; D014183:Transplantation, Heterologous
chemical_list: D049452:Green Fluorescent Proteins