title: Real-time high dynamic range laser scanning microscopy.
abstract: In conventional confocal/multiphoton fluorescence microscopy, images are typically acquired under ideal settings and after extensive optimization of parameters for a given structure or feature, often resulting in information loss from other image attributes. To overcome the problem of selective data display, we developed a new method that extends the imaging dynamic range in optical microscopy and improves the signal-to-noise ratio. Here we demonstrate how real-time and sequential high dynamic range microscopy facilitates automated three-dimensional neural segmentation. We address reconstruction and segmentation performance on samples with different size, anatomy and complexity. Finally, in vivo real-time high dynamic range imaging is also demonstrated, making the technique particularly relevant for longitudinal imaging in the presence of physiological motion and/or for quantification of in vivo fast tracer kinetics during functional imaging.
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center, 185 Cambridge Street, Boston, Massachusetts 02114, USA.
authors.forename: C
authors.initials: C
authors.lastname: Vinegoni
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center, 185 Cambridge Street, Boston, Massachusetts 02114, USA.
authors.forename: C
authors.initials: C
authors.lastname: Leon Swisher
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center, 185 Cambridge Street, Boston, Massachusetts 02114, USA.|Department of Neurological, Biomedical and Movement Sciences, University of Verona, Strada Le Grazie 8, 37134 Verona, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Fumene Feruglio
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center, 185 Cambridge Street, Boston, Massachusetts 02114, USA.
authors.forename: R J
authors.initials: RJ
authors.lastname: Giedt
authors.affiliation: Center for Brain Science, Department of Molecular and Cell Biology, Harvard University, 52 Oxford Street, Cambridge, Massachusetts 02138, USA.
authors.forename: D L
authors.initials: DL
authors.lastname: Rousso
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center, 185 Cambridge Street, Boston, Massachusetts 02114, USA.
authors.forename: S
authors.initials: S
authors.lastname: Stapleton
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center, 185 Cambridge Street, Boston, Massachusetts 02114, USA.
authors.forename: R
authors.initials: R
authors.lastname: Weissleder
mesh_terms: D000465:Algorithms; D000818:Animals; D001921:Brain / Q000473:pathology; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton; D059629:Signal-To-Noise Ratio