title: A Centrifugal Microfluidic Platform That Separates Whole Blood Samples into Multiple Removable Fractions Due to Several Discrete but Continuous Density Gradient Sections.
abstract: We present a miniaturized centrifugal platform that uses density centrifugation for separation and analysis of biological components in small volume samples (~5 μL). We demonstrate the ability to enrich leukocytes for on-disk visualization via microscopy, as well as recovery of viable cells from each of the gradient partitions. In addition, we simplified the traditional Modified Wright-Giemsa staining by decreasing the time, volume, and expertise involved in the procedure. From a whole blood sample, we were able to extract 95.15% of leukocytes while excluding 99.8% of red blood cells. This platform has great potential in both medical diagnostics and research applications as it offers a simpler, automated, and inexpensive method for biological sample separation, analysis, and downstream culturing.
authors.affiliation: Sandia National Laboratories, Livermore, California, United States of America.|University of Texas Medical Branch, Galveston, Texas, United States of America.
authors.forename: Scott T
authors.initials: ST
authors.lastname: Moen
authors.affiliation: University of Texas Medical Branch, Galveston, Texas, United States of America.
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Hatcher
authors.affiliation: Sandia National Laboratories, Livermore, California, United States of America.|University of Texas Medical Branch, Galveston, Texas, United States of America.
authors.forename: Anup K
authors.initials: AK
authors.lastname: Singh
mesh_terms: D001773:Blood Cells / Q000166:cytology*; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D002499:Centrifugation, Density Gradient / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods