title: Proteomic changes in the liver of Channa striatus in response to high temperature stress.
abstract: The present study was undertaken to investigate the proteomic changes in the liver of murrel Channa striatus exposed to high temperature stress. Fishes were exposed to 36°C for 4 days and liver proteome changes were analyzed using gel- based proteomics, i.e. 2DE, MALDI-TOF/TOF-MS, and validation by transcript analysis. The study showed, besides others, increased abundance of two sets of proteins, the antioxidative enzymes superoxide dismutase (SOD), ferritin, cellular retinol binding protein (CRBP), glutathione-S-transferase (GST), and the chaperones HSP60 and protein disulfide isomerase; this was validated by transcript analysis. The proteome data are available via ProteomeXchange with identifier PXD002608. Further, gene expression analysis was also carried out in the fishes exposed to thermal stress for longer durations (30 days experimental exposure in laboratory and for 30 days beyond, taking Channa collected from a hot spring runoff at 36-38°C); sod, gst, crbp, and hsp60 were found to continue to remain upregulated at eight-, 2.5-, 2.4-, and 2.45-fold, respectively, in the hot spring runoff fish. Pathway analysis showed that the upregulations of the antioxidant enzymes as well as molecular chaperones are induced by the transcription factor Nrf2 (nuclear factor erythroid 2-related factor 2). Thus, while short-term heat stress tolerance involves the antioxidative enzymes SOD, ferritin, CRBP, GST, and chaperones HSP60 and protein disulfide isomerase, adaptation under chronic heat stress is associated with SOD, CRBP, GST, and HSP60.
authors.affiliation: ICAR-Central Inland Fisheries Research Institute, FREM Division, Barrackpore, Kolkata, India.
authors.forename: Arabinda
authors.initials: A
authors.lastname: Mahanty
authors.affiliation: KIIT School of Biotechnology, KIIT University, Bhubaneswar, Odisha, India.
authors.forename: Gopal Krishna
authors.initials: GK
authors.lastname: Purohit
authors.affiliation: ICAR-Central Inland Fisheries Research Institute, FREM Division, Barrackpore, Kolkata, India.
authors.forename: Sudeshna
authors.initials: S
authors.lastname: Banerjee
authors.affiliation: ICAR-Central Inland Fisheries Research Institute, FREM Division, Barrackpore, Kolkata, India.
authors.forename: Dhanasekar
authors.initials: D
authors.lastname: Karunakaran
authors.affiliation: KIIT School of Biotechnology, KIIT University, Bhubaneswar, Odisha, India.
authors.forename: Sasmita
authors.initials: S
authors.lastname: Mohanty
authors.affiliation: ICAR-Central Inland Fisheries Research Institute, FREM Division, Barrackpore, Kolkata, India.
authors.forename: Bimal Prasanna
authors.initials: BP
authors.lastname: Mohanty
mesh_terms: D000818:Animals; D018834:Chaperonin 60 / Q000235:genetics; D005399:Fishes / Q000033:anatomy & histology*; D020869:Gene Expression Profiling; D005982:Glutathione Transferase / Q000235:genetics; D018869:Heat-Shock Response / Q000235:genetics*; D006358:Hot Temperature; D008099:Liver / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D054840:Retinol-Binding Proteins, Cellular / Q000235:genetics; D013482:Superoxide Dismutase / Q000235:genetics; D015854:Up-Regulation
chemical_list: D018834:Chaperonin 60; D054840:Retinol-Binding Proteins, Cellular; D013482:Superoxide Dismutase; D005982:Glutathione Transferase
keywords: Antioxidative enzymes; Channa striatus; High temperature stress; Liver proteome; Stress response