title: Viscerotropic velogenic Newcastle disease virus replication in feathers of infected chickens.
abstract: Newcastle disease viruses (NDVs) cause systemic diseases in chickens with high mortality. However, little is known about persistence of NDVs in contaminated tissues from infected birds. In this study, we examined viral replication in the feather pulp of chickens inoculated with viscerotropic velogenic NDV (vvNDV) genotype VII. Reverse transcription real-time PCR and immunohistochemistry were used to investigate viral persistence in the samples. vvNDV was detected in the oropharynx and cloaca and viral antigens were detected in the feathers, suggesting that feathers act as sources of viral transmission.
authors.affiliation: Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Dong-Hun
authors.initials: DH
authors.lastname: Lee
authors.affiliation: Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Kwon
authors.affiliation: Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Jin-Yong
authors.initials: JY
authors.lastname: Noh
authors.affiliation: Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Jae-Keun
authors.initials: JK
authors.lastname: Park
authors.affiliation: Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Seong-Su
authors.initials: SS
authors.lastname: Yuk
authors.affiliation: Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Tseren-Ochir
authors.initials: TO
authors.lastname: Erdene-Ochir
authors.affiliation: Laboratory of Veterinary Anatomy, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Sang-Soep
authors.initials: SS
authors.lastname: Nahm
authors.affiliation: Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs, Anyang 14089, Korea.
authors.forename: Yong-Kuk
authors.initials: YK
authors.lastname: Kwon
authors.affiliation: Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Sang-Won
authors.initials: SW
authors.lastname: Lee
authors.affiliation: Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Chang-Seon
authors.initials: CS
authors.lastname: Song
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002645:Chickens; D002988:Cloaca / Q000821:virology; D005241:Feathers / Q000821:virology*; D050296:Microbial Viability; D009521:Newcastle Disease / Q000635:transmission / Q000821:virology*; D009522:Newcastle disease virus / Q000302:isolation & purification / Q000502:physiology*; D009960:Oropharynx / Q000821:virology; D011201:Poultry Diseases / Q000635:transmission / Q000821:virology*; D014779:Virus Replication / Q000502:physiology*
chemical_list: D000956:Antigens, Viral
keywords: Newcastle disease virus; chicken; feathers