title: Plasma biomarkers of SIRS and MODS associated with canine babesiosis.
abstract: Canine babesiosis is a tick-borne disease caused by the haemoprotozoan parasites of the genus Babesia. Early detection of systemic inflammatory response syndrome (SIRS) and multiple organ dysfunction syndrome (MODS) is of major importance in clinical practice for providing information about severity and outcomes of the disease and therapy. Plasma samples were taken at admission from five dogs with uncomplicated babesiosis caused by B. canis canis, five dogs with babesiosis and SIRS, five dogs with babesiosis and MODS, and five healthy dogs. After two-dimensional electrophoresis and capillary reversed - phase liquid chromatography coupled online with tandem mass spectrometry, 68 differentially expressed spots with level of significance P<0.05 were detected between groups. SIRS in babesiosis was characterised by increases in paraoxonase 1 and apoA-I, whereas MODS with decrease of complement inhibitors leading to prolonged complement activation and decrease of vitamin D binding protein due to haemolysis and activation of the coagulation cascade.
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000 Zagreb, Croatia. Electronic address: jkules@vef.hr.
authors.forename: J
authors.initials: J
authors.lastname: Kuleš
authors.affiliation: Proteomics Unit, Universitary Hospital Virgen de la Arrixaca, Ctra. Madrid-Cartagena, s/n, 30120 El Palmar, Murcia, Spain.
authors.forename: C
authors.initials: C
authors.lastname: de Torre-Minguela
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000 Zagreb, Croatia.
authors.forename: R
authors.initials: R
authors.lastname: Barić Rafaj
authors.affiliation: Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000 Zagreb, Croatia.
authors.forename: J
authors.initials: J
authors.lastname: Gotić
authors.affiliation: Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000 Zagreb, Croatia.
authors.forename: P
authors.initials: P
authors.lastname: Nižić
authors.affiliation: Department of Animal Medicine and Surgery, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30 100, Murcia, Spain.
authors.forename: J J
authors.initials: JJ
authors.lastname: Ceron
authors.affiliation: Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000 Zagreb, Croatia.
authors.forename: V
authors.initials: V
authors.lastname: Mrljak
mesh_terms: D000818:Animals; D001403:Babesia / Q000502:physiology*; D001404:Babesiosis / Q000175:diagnosis* / Q000469:parasitology; D015415:Biomarkers / Q000097:blood; D002853:Chromatography, Liquid / Q000662:veterinary; D004283:Dog Diseases / Q000175:diagnosis* / Q000469:parasitology; D004285:Dogs; D015180:Electrophoresis, Gel, Two-Dimensional / Q000662:veterinary; D009102:Multiple Organ Failure / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D018746:Systemic Inflammatory Response Syndrome / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D053719:Tandem Mass Spectrometry / Q000662:veterinary
chemical_list: D015415:Biomarkers
keywords: Babesiosis; Multiple organ dysfunction syndrome (MODS); Proteomics; Systemic inflammatory response syndrome (SIRS)