title: Fertility response in postpartum anoestrus buffaloes (Bubalus bubalis) using modified Ovsynch based timed insemination protocols.
abstract: OBJECTIVE
  Suboestrus constitutes the largest factor responsible for poor reproductive efficiency in buffaloes. Therefore, oestrus synchronization (OS) and fixed-time artificial insemination (AI) is considered an alternative approach to enhance reproductive efficiency in buffaloes. Thus, the present study was carried out to study the efficacy of modified Ovsynch protocol with fixed time insemination in post-partum anoestrus buffaloes.
  
  
  METHODS
  Total 50 post-partum anoestrus dairy buffaloes were selected and randomly divided into 5 Groups, each comprising ten animals (n=10). Animals of Group I received buserelin acetate 10 μg(GnRH) at day 0 and 9, and prostaglandin F2α (PGF2α) at day 7; in Group II similar to Group I except double dose of Gn RH (20 μg) at day 0; in Group III, similar to Group I plus additional administration of insulin on day 0, 1 and 2; in Group IV, similar to Group II plus additional administration of insulin on day 0,1 and 2; in Group V similar to Group I except replacement of first Gn RH with insulin on day 0,1 and 2. Animal of all groups were inseminated at fixed time using frozen semen at 60 h and 72 h after PGF2α administration and confirmed for pregnancy at day 60 post-insemination.
  
  
  RESULTS
  The results revealed satisfactory and comparable synchronization of oestrus (60-80%) and conception rate (20-40%) among the various treatment groups in the present study. The better synchronization was observed in modified protocols. However, non-significantly higher conception rate was recorded in modified Ovsynch protocol (40% each in Group II-V) as compared to 20% in Group-I (p>0.05).
  
  
  CONCLUSIONS
  In this study comparatively higher OS and conception following insulin modified Ovsynch based timed AI protocols in post-partum anoestrus buffaloes was found satisfactory and comparable.
authors.affiliation: Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science & Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh, India.
authors.forename: K K
authors.initials: KK
authors.lastname: Gupta
authors.affiliation: Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science & Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh, India.
authors.forename: S N
authors.initials: SN
authors.lastname: Shukla
authors.affiliation: Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science & Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh, India.
authors.forename: P
authors.initials: P
authors.lastname: Inwati
authors.affiliation: Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science & Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh, India.
authors.forename: O P
authors.initials: OP
authors.lastname: Shrivastava
keywords: anoestrus buffaloes; fertility; insulin; ovsynch synchronization; timed artificial insemination