title: Nuclear factor-κB-dependent microRNA-130a upregulation promotes cervical cancer cell growth by targeting phosphatase and tensin homolog.
abstract: Nuclear factor-κB (NF-κB) may activate a series of gene transcription control cellular signaling pathways whose products are components in a wide range of biological processes. MicroRNAs, a group of non-coding endogenous ones, may regulate gene expression and plays specific roles in tumorigenesis. Using human cervical cancer cell lines, we explored whether NF-κB regulates the expression of microRNA-130a (miR-130a) through binding elements in the miR-130a promoter region. We found that miR-130a accelerates cervical cancer cell proliferation by targeting the phosphatase and tensin homolog on chromosome 10 (PTEN). Further, NF-κB activates both HeLa and CaSki cell growth by upregulating miR-130a. In addition, by targeting PTEN 3' untranslated region, miR-130a might increase cell growth and initiate protein kinase B (AKT) pathway activation. Lastly, PTEN protein was upregulated in response to NF-κB overexpression and downregulated in response to NF-κB inhibition. Compared to total AKT protein level, p-AKT was downregulated by NF-κB overexpression while upregulated by NF-κB inhibition, indicating PTEN pathway activated and affected by NF-κB. Taken together, our findings shed new light on the NF-κB/miR-130a/PTEN pathway in cervical cancer and add new insight regarding the carcinogenesis of cervical cancer.
authors.affiliation: Department of Oncology, The Second Xiangya Hospital of Central South University, China. Electronic address: omo_2006@126.com.
authors.forename: Yeqian
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Department of Vasculocardiology, The Second Xiangya Hospital of Central South University, China. Electronic address: zhoushenghua_guo@163.com.
authors.forename: Shenghua
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Cancer Research Institute of Central South University, China. Electronic address: ligy@xysm.net.
authors.forename: Guiyuan
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Oncology, The Second Xiangya Hospital of Central South University, China. Electronic address: huchunh@medmail.com.cn.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Hu
authors.affiliation: Department of Oncology, The Second Xiangya Hospital of Central South University, China. Electronic address: zouwen29w@126.com.
authors.forename: Wen
authors.initials: W
authors.lastname: Zou
authors.affiliation: Department of Oncology, The Second Xiangya Hospital of Central South University, China. Electronic address: 25386132@qq.com.
authors.forename: Haixia
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Oncology, The First People's Hospital of YueYang, China. Electronic address: 39904963@qq.com.
authors.forename: Lili
authors.initials: L
authors.lastname: Sun
mesh_terms: D020413:3' Untranslated Regions; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D015398:Signal Transduction; D015854:Up-Regulation; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D020413:3' Untranslated Regions; C554019:MIRN130 microRNA, human; D035683:MicroRNAs; D016328:NF-kappa B; D012334:RNA, Neoplasm; D051057:Proto-Oncogene Proteins c-akt; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human
keywords: AKT; Cervical cancer; NF-κB; PTEN; Tumorigenesis; miR-130a