title: Modified gold nanoparticles for intracellular delivery of anti-liver cancer siRNA.
abstract: To overcome the rapid enzymatic degradation and low transfection efficiency of siRNA, the delivery carriers for siRNA is a therapeutic demand to increase its stability. Gold nanoparticles (AuNPs) modified by branched polyethyleneimine (bPEI) were developed as an efficient and safe intracellular delivery carriers for siRNA. The current study implied that siRNA designed against an oncogene c-Myc could be delivered by a modified AuNPs complex without significant cytotoxicity. The comparative semi-quantitative and quantitative real time PCR were used to measure the c-Myc gene expression after transfection with naked siRNA and siRNA/bPEI/AuNPs, but AuNPs interfered with PCR. However, the c-Myc protein translation was successfully detected in the transfected HuH7 cells with naked siRNA and siRNA/bPEI/AuNPs and it was found to be inhibited by siRNA/bPEI/AuNPs more than naked siRNA. The results validate the successful silencing of c-Myc gene. Accordingly, it may confirm the promising and effective delivery of siRNA by bPEI/AuNPs. The complex enhances the cellular uptake of siRNA without significant cytotoxicity and confirms that bPEI modified AuNPs could be used as a good candidate for safe cellular delivery of siRNA.
authors.affiliation: Chemistry Department, Faculty of Science, Benha University, Benha, Egypt; Nanomedicine and Tissue Engineering Laboratory, Medical Research Centre of excellence, National Research Centre (NRC), Cairo, Egypt.
authors.forename: Hanan
authors.initials: H
authors.lastname: Shaat
authors.affiliation: Nanomedicine and Tissue Engineering Laboratory, Medical Research Centre of excellence, National Research Centre (NRC), Cairo, Egypt; Ceramics Department, NRC, Dokki, Cairo, Egypt,. Electronic address: amany.mostafa@univ-rennes1.fr.
authors.forename: Amany
authors.initials: A
authors.lastname: Mostafa
authors.affiliation: Chemistry Department, Faculty of Science, Benha University, Benha, Egypt.
authors.forename: Moustafa
authors.initials: M
authors.lastname: Moustafa
authors.affiliation: Cancer Biology and Genetics Laboratory Centre of Excellence for Advanced Sciences, NRC, Cairo, Egypt; Biochemistry Department, NRC, Dokki, Cairo, Egypt.
authors.forename: Amira
authors.initials: A
authors.lastname: Gamal-Eldeen
authors.affiliation: Nanomedicine and Tissue Engineering Laboratory, Medical Research Centre of excellence, National Research Centre (NRC), Cairo, Egypt; Ceramics Department, NRC, Dokki, Cairo, Egypt.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Emam
authors.affiliation: Zoology Department, Faculty of Science, Ain-Shams University, Cairo, Egypt.
authors.forename: Enas
authors.initials: E
authors.lastname: El-Hussieny
authors.affiliation: Biomedical Informatics and Chemo-Informatics Laboratory, Center of Excellence for advanced Sciences, NRC, Dokki, Cairo, Egypt,; Informatics and System Department, NRC, Dokki, Cairo, Egypt. Electronic address: mahef@aucegypt.edu.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Elhefnawi
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002951:Citrates / Q000737:chemistry; D015870:Gene Expression; D006046:Gold / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D008055:Lipids / Q000008:administration & dosage; D008113:Liver Neoplasms / Q000378:metabolism; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D002951:Citrates; D008055:Lipids; C086724:Lipofectamine; C489427:MYC protein, human; D016271:Proto-Oncogene Proteins c-myc; D034741:RNA, Small Interfering; C514290:trisodium citrate; D006046:Gold
keywords: Gene delivery; Gold nanoparticles; Polyelectrolyte complex; Polyethyleneimine; Small interfering RNA (siRNA); c-Myc