title: [IL-33 promotes degranulation of mouse bone marrow-derived mast cells and release of cytokines IL-1β, IL-6 and TNF-α].
abstract: OBJECTIVE
  To investigate the effect of interleukin 33 (IL-33) on degranulation and cytokine release of mouse bone marrow-derived mast cells (BMMCs).
  
  
  METHODS
  Mouse BMMCs were isolated and stimulated by 0, 10, 20, 50 ng/mL IL-33. The expression of c-Kit was assessed by Western blotting. Beta-hexosaminidase content in culture supernatant was evaluated indirectly through the absorbance value of the product of the reaction between chromogenix substrate and β-hexosaminidase. The levels of histamine and cytokines (IL-1β, IL-6 and TNF-α) in culture supernatant were examined by ELISA.
  
  
  RESULTS
  IL-33 induced the expression of c-Kit in BMMCs. Treatments with different concentrations of IL-33 for 30 minutes induced the degranulation of BMMCs to release β-hexosaminidase and histamine in a dose-dependent manner. IL-33 induced the release of IL-1β, IL-6 and TNF-α in BMMCs after treatments for 24 hours; the peak values of the three kinds of cytokines were got respectively in 50, 50 and 20 ng/mL IL-33 treatment groups.
  
  
  CONCLUSIONS
  IL-33 could induce the degranulation of mast cells and the release of cytokines (IL-1β, IL-6 and TNF-α).
authors.affiliation: New Drug Screening Center, China Pharmaceutical University, Nanjing 210009; School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Zhou
authors.affiliation: New Drug Screening Center, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: New Drug Screening Center, China Pharmaceutical University, Nanjing 210009, China. *Corresponding author, E-mail: shangjing21cn@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Shang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism*; D002478:Cells, Cultured; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D000067596:Interleukin-33 / Q000235:genetics / Q000378:metabolism*; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: C587672:IL1B protein, mouse; D053583:Interleukin-1beta; D000067596:Interleukin-33; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha