title: Meiotic recombination is suppressed near the histone-defined border of euchromatin and heterochromatin on chromosome 2L of Drosophila melanogaster.
abstract: In Drosophila melanogaster, the borders between pericentric heterochromatin and euchromatin on the major chromosome arms have been defined in various ways, including chromatin-specific histone modifications, the binding patterns of heterochromatin-enriched chromosomal proteins, and various cytogenetic techniques. Elucidation of the genetic properties that independently define the different chromatin states associated with heterochromatin and euchromatin should help refine the boundary. Since meiotic recombination is present in euchromatin, but absent in heterochromatin, it constitutes a key genetic property that can be observed transitioning between chromatin states. Using P element insertion lines marked with a su(Hw) insulated mini-white gene, meiotic recombination was found to transition in a region consistent with the H3K9me2 transition observed in ovaries.
authors.affiliation: a Department of Biology, York University, Toronto, ON M3J 1P3, Canada.
authors.forename: Alistair B
authors.initials: AB
authors.lastname: Coulthard
authors.affiliation: a Department of Biology, York University, Toronto, ON M3J 1P3, Canada.
authors.forename: Rhodri W
authors.initials: RW
authors.lastname: Taylor-Kamall
authors.affiliation: b Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC V5A 1S6, Canada.
authors.forename: Graham
authors.initials: G
authors.lastname: Hallson
authors.affiliation: a Department of Biology, York University, Toronto, ON M3J 1P3, Canada.
authors.forename: Anna
authors.initials: A
authors.lastname: Axentiev
authors.affiliation: b Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC V5A 1S6, Canada.
authors.forename: Don A
authors.initials: DA
authors.lastname: Sinclair
authors.affiliation: b Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC V5A 1S6, Canada.
authors.forename: Barry M
authors.initials: BM
authors.lastname: Honda
authors.affiliation: a Department of Biology, York University, Toronto, ON M3J 1P3, Canada.
authors.forename: Arthur J
authors.initials: AJ
authors.lastname: Hilliker
mesh_terms: D000818:Animals; D059006:Chromosomes, Insect / Q000235:genetics; D019175:DNA Methylation; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D022041:Euchromatin / Q000235:genetics*; D005260:Female; D006570:Heterochromatin / Q000235:genetics*; D006657:Histones / Q000235:genetics; D059765:Homologous Recombination; D008540:Meiosis; D010053:Ovary
chemical_list: D004251:DNA Transposable Elements; D022041:Euchromatin; D006570:Heterochromatin; D006657:Histones
keywords: Drosophila; euchromatic–heterochromatic border; frontières l’hétérochromatine et l’euchromatine; heterochromatin; hétérochromatine; meiotic recombination; recombinaison méiotique