title: Decoding Polo-like kinase 1 signaling along the kinetochore-centromere axis.
abstract: Protein kinase signaling along the kinetochore-centromere axis is crucial to assure mitotic fidelity, yet the details of its spatial coordination are obscure. Here, we examined how pools of human Polo-like kinase 1 (Plk1) within this axis control signaling events to elicit mitotic functions. To do this, we restricted active Plk1 to discrete subcompartments within the kinetochore-centromere axis using chemical genetics and decoded functional and phosphoproteomic signatures of each. We observe distinct phosphoproteomic and functional roles, suggesting that Plk1 exists and functions in discrete pools along this axis. Deep within the centromere, Plk1 operates to assure proper chromosome alignment and segregation. Thus, Plk1 at the kinetochore is a conglomerate of an observable bulk pool coupled with additional functional pools below the threshold of microscopic detection or resolution. Although complex, this multiplicity of locales provides an opportunity to decouple functional and phosphoproteomic signatures for a comprehensive understanding of Plk1's kinetochore functions.
authors.affiliation: Department of Medicine, Hematology/Oncology Division, University of Wisconsin-Madison, Madison, Wisconsin, USA.|University of Wisconsin Carbone Cancer Center, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Robert F
authors.initials: RF
authors.lastname: Lera
authors.affiliation: Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.|Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Gregory K
authors.initials: GK
authors.lastname: Potts
authors.affiliation: Department of Biology, University of North Carolina, Chapel Hill, North Carolina, USA.
authors.forename: Aussie
authors.initials: A
authors.lastname: Suzuki
authors.affiliation: Department of Medicine, Hematology/Oncology Division, University of Wisconsin-Madison, Madison, Wisconsin, USA.|University of Wisconsin Carbone Cancer Center, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: James M
authors.initials: JM
authors.lastname: Johnson
authors.affiliation: Department of Biology, University of North Carolina, Chapel Hill, North Carolina, USA.
authors.forename: Edward D
authors.initials: ED
authors.lastname: Salmon
authors.affiliation: Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.|Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.|Genome Center, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Joshua J
authors.initials: JJ
authors.lastname: Coon
authors.affiliation: Department of Medicine, Hematology/Oncology Division, University of Wisconsin-Madison, Madison, Wisconsin, USA.|University of Wisconsin Carbone Cancer Center, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Mark E
authors.identifier: 0000-0002-4215-7722
authors.initials: ME
authors.lastname: Burkard
mesh_terms: D018797:Cell Cycle Proteins / Q000378:metabolism*; D002503:Centromere / Q000378:metabolism*; D006801:Humans; D018386:Kinetochores / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D015398:Signal Transduction; D000097869:Polo-Like Kinase 1
chemical_list: D018797:Cell Cycle Proteins; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D000097869:Polo-Like Kinase 1