title: Opsonophagocytic Assay To Evaluate Immunogenicity of Nontyphoidal Salmonella Vaccines.
abstract: Nontyphoidal Salmonella (NTS) invasive infections are an important cause of morbidity and mortality in sub-Saharan Africa. Several vaccines are in development to prevent these infections. We describe an NTS opsonophagocytic killing assay that uses HL-60 cells and baby rabbit complement to quantify functional antibodies elicited by candidate NTS vaccines.
authors.affiliation: Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland, USA.|Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA.
authors.forename: Girish
authors.initials: G
authors.lastname: Ramachandran
authors.affiliation: Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland, USA.|Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland, USA.
authors.forename: Mary Adetinuke
authors.initials: MA
authors.lastname: Boyd
authors.affiliation: Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland, USA.|Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: MacSwords
authors.affiliation: Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland, USA.|Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA.
authors.forename: Ellen E
authors.initials: EE
authors.lastname: Higginson
authors.affiliation: Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland, USA.|Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA.
authors.forename: Raphael
authors.initials: R
authors.lastname: Simon
authors.affiliation: Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland, USA.|Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA.
authors.forename: James E
authors.initials: JE
authors.lastname: Galen
authors.affiliation: Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland, USA.|Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland, USA.
authors.forename: Marcela F
authors.initials: MF
authors.lastname: Pasetti
authors.affiliation: Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland, USA.|Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA.|Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland, USA.
authors.forename: Myron M
authors.initials: MM
authors.lastname: Levine
authors.affiliation: Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland, USA stennant@medicine.umaryland.edu.|Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA.
authors.forename: Sharon M
authors.initials: SM
authors.lastname: Tennant
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology*; D002675:Child, Preschool; D003165:Complement System Proteins / Q000276:immunology; D018922:HL-60 Cells; D006801:Humans; D000071497:Immunogenicity, Vaccine; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D010587:Phagocytosis; D011817:Rabbits; D012475:Salmonella / Q000276:immunology*; D012480:Salmonella Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control; D022562:Salmonella Vaccines / Q000276:immunology*; D012486:Salmonella typhimurium / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D022562:Salmonella Vaccines; D003165:Complement System Proteins