title: Antimicrobial, Anti-inflammatory and Antioxidant Activities of Jatropha multifida L. (Euphorbiaceae).
abstract: BACKGROUND
  Jatropha multifida is used in Togolease folk medicine for the healing of chronic wounds.
  
  
  OBJECTIVE
  This study aims to investigate antibacterial, anti-inflammatory and antioxidant activities of the leaves ethanolic extract.
  
  
  METHODS
  The antimicrobial activity was assayed by National Committee for Clinical Laboratory Standards broth microdilution method on strains of Staphylococcus aureus and Pseudomoas aeruginosa isolated from wounds, whereas the anti-inflammatory activity was performed by carrageenan and histamine induced paw edema method in rat modele. The 2, 2-diphenyl-1picrylhydrazyl (DPPH) free radical scavenging and ferric reducing antioxidant power (FRAP) were used for the antioxidant activity.
  
  
  RESULTS
  The antibacterial assay showed an in vitro growth inhibition of P. aeruginosa and S. aureus in dose-dependent manner, with minimum inhibitory concentration values ranging from 2.5 to 3.12 mg/mL for S. aureus and from 6.25 to 12.5 mg/mL for P. aeruginosa. The maximum paw anti-inflammatory effect occurred after 3 and 5 h administration of histamine and carrageenan, respectively. The DPPH radical scavenging and the FRAP assays yielded weak antioxidant activity.
  
  
  CONCLUSIONS
  J. multifida possesses antibacterial and anti-inflammatory activities that could justify the use of the plant for the treatment of wounds in the folk medicine.
  
  
  CONCLUSIONS
  Antibacterial on germs isolated from wound, anti-inflammatory and antioxidant activities of Jatropha multifida were assayed by NCCLS broth method, carrageenan and histamine, DPPH and FRAP respectively. The results indicated that Jatropha multifida possesses antibacterial and anti-inflammatory and weak antioxidant activities that could justify its use for the treatment of wounds in the folk medicine.
authors.affiliation: Laboratoire de Microbiologie et de Contrôle de Qualité des Denrées Alimentaires (LAMICODA), Togo; Centre de Recherche et de Formation sur les Plantes Médicinales (CERFOPLAM), Togo; Centre de Recherche en Agriculture Biologique et Substances Naturelles (CRABISNA), Togo.
authors.forename: Kokou
authors.initials: K
authors.lastname: Anani
authors.affiliation: Laboratoire de Microbiologie et de Contrôle de Qualité des Denrées Alimentaires (LAMICODA), Togo; Centre de Recherche et de Formation sur les Plantes Médicinales (CERFOPLAM), Togo; Centre de Recherche en Agriculture Biologique et Substances Naturelles (CRABISNA), Togo.
authors.forename: Yao
authors.initials: Y
authors.lastname: Adjrah
authors.affiliation: Laboratoire de Microbiologie et de Contrôle de Qualité des Denrées Alimentaires (LAMICODA), Togo; Centre de Recherche et de Formation sur les Plantes Médicinales (CERFOPLAM), Togo.
authors.forename: Yaovi
authors.initials: Y
authors.lastname: Améyapoh
authors.affiliation: Laboratoire de Microbiologie et de Contrôle de Qualité des Denrées Alimentaires (LAMICODA), Togo; Centre de Recherche et de Formation sur les Plantes Médicinales (CERFOPLAM), Togo; Centre de Recherche en Agriculture Biologique et Substances Naturelles (CRABISNA), Togo.
authors.forename: Simplice Damintoti
authors.initials: SD
authors.lastname: Karou
authors.affiliation: Centre de Recherche et de Formation sur les Plantes Médicinales (CERFOPLAM), Togo; Laboratoire de Physiologie et de Pharmacologie, Faculté des Sciences, Université de Lomé, B.P. 1515, Lomé, Togo.
authors.forename: Amegnona
authors.initials: A
authors.lastname: Agbonon
authors.affiliation: Laboratoire de Microbiologie et de Contrôle de Qualité des Denrées Alimentaires (LAMICODA), Togo; Centre de Recherche et de Formation sur les Plantes Médicinales (CERFOPLAM), Togo.
authors.forename: Comlan
authors.initials: C
authors.lastname: de Souza
authors.affiliation: Centre de Recherche et de Formation sur les Plantes Médicinales (CERFOPLAM), Togo; Laboratoire de Physiologie et de Pharmacologie, Faculté des Sciences, Université de Lomé, B.P. 1515, Lomé, Togo.
authors.forename: Messanvi
authors.initials: M
authors.lastname: Gbeassor
keywords: Anti-inflammatory; Antibacterial; Antioxidant; Jatropha multifida; wound