title: Inhibition of auxin transport and auxin signaling and treatment with far red light induces root coiling in the phospholipase-A mutant ppla-I-1. Significance for surface penetration?
abstract: When grown on a non-penetretable at a surface angle of 45°, Arabidopsis roots form wave-like structures and, in wild type rarely, but in certain mutants the tip root even may form circles. These circles are called coils. The formation of coils depends on the complex interaction of circumnutation, gravitropism and negative thigmotropism where - at least - gravitropism is intimately linked to auxin transport and signaling. The knockout mutant of patatin-related phospholipase-AI-1 (pplaI-1) is an auxin-signaling mutant which forms moderately increased numbers of coils on tilted agar plates. We tested the effects of the auxin efflux transport inhibitor NPA (1-naphthylphtalamic acid) and of the influx transport inhibitor 1-NOA (1-naphthoxyacetic acid) which both further increased root coil formation. The pPLAI-1 inhibitors HELSS (haloenol lactone suicide substrate=E-6-(bromomethylene)tetrahydro-3-(1-naphthalenyl)-2H-pyran-2-one) and ETYA (eicosatetraynoic acid) which are auxin signaling inhibitors also increased coil formation. In addition, far red light treatment increased coil formation. The results point out that a disturbance of auxin transport and signaling is one potential cause for root coils. As we show that the mutant pplaI-1 penetrates horizontal agar plates better than wild type plants root movements may help penetrating the soil.
authors.affiliation: Leibniz Universität Hannover, Institut für Gartenbauliche Produktionssysteme, Abt. Molekulare Ertragsphysiologie, Herrenhäuser Str. 2, D-30419 Hannover, Germany; Universität Hamburg Fakultät für Mathematik, Informatik und Naturwissenschaften, Biologie, Biozentrum Klein Flottbek, Ohnhorststr. 18, 22609 Hamburg, Germany. Electronic address: perrineau@uni-hamburg.de.
authors.forename: F
authors.initials: F
authors.lastname: Perrineau
authors.affiliation: Leibniz Universität Hannover, Institut für Gartenbauliche Produktionssysteme, Abt. Molekulare Ertragsphysiologie, Herrenhäuser Str. 2, D-30419 Hannover, Germany. Electronic address: rinukshi@yahoo.co.in.
authors.forename: R
authors.initials: R
authors.lastname: Wimalasekera
authors.affiliation: Leibniz Universität Hannover, Institut für Gartenbauliche Produktionssysteme, Abt. Molekulare Ertragsphysiologie, Herrenhäuser Str. 2, D-30419 Hannover, Germany; Al Azhar Indonesia University, Department of Biology, Sisingamangaraja, 12110 Jakarta, Indonesia. Electronic address: eyunus01@yahoo.com.
authors.forename: Y
authors.initials: Y
authors.lastname: Effendi
authors.affiliation: Leibniz Universität Hannover, Institut für Gartenbauliche Produktionssysteme, Abt. Molekulare Ertragsphysiologie, Herrenhäuser Str. 2, D-30419 Hannover, Germany. Electronic address: scherer@zier.uni-hannover.de.
authors.forename: G F E
authors.initials: GF
authors.lastname: Scherer
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D001692:Biological Transport; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D007210:Indoleacetic Acids / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007259:Infrared Rays; D009154:Mutation; D010937:Plant Growth Regulators / Q000037:antagonists & inhibitors / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D002265:Carboxylic Ester Hydrolases; C522182:PLAI protein, Arabidopsis
keywords: 1-NOA (1-naphthoxyacetic acid); 1-NPA (1-naphthylphtalamic acid); 2-NOA (2-naphthoxyacetic acid); ETYA (eicosatetraynoic acid); HELSS (haloenol lactone suicide substrate=E-6-(bromomethylene)tetrahydro-3-(1-naphthalenyl)-2H-pyran-2-one); Red light; pPLAI-1 (patatin-related phospholipase-AI-1)