title: Tailoring Novel PTFE Surface Properties: Promoting Cell Adhesion and Antifouling Properties via a Wet Chemical Approach.
abstract: Many biomaterials used for tissue engineering applications lack cell-adhesiveness and, in addition, are prone to nonspecific adsorption of proteins. This is especially important for blood-contacting devices such as vascular grafts and valves where appropriate surface properties should inhibit the initial attachment of platelets and promote endothelial cell colonization. As a consequence, the long-term outcome of the implants would be improved and the need for anticoagulation therapy could be reduced or even abolished. Polytetrafluoroethylene (PTFE), a frequently used polymer for various medical applications, was wet-chemically activated and subsequently modified by grafting the endothelial cell (EC) specific peptide arginine-glutamic acid-aspartic acid-valine (REDV) using a bifunctional polyethylene glycol (PEG)-spacer (known to reduce platelet and nonspecific protein adhesion). Modified and control surfaces were both evaluated in terms of EC adhesion, colonization, and the attachment of platelets. In addition, samples underwent bacterial challenges. The results strongly suggested that PEG-mediated peptide immobilization renders PTFE an excellent substrate for cellular growth while simultaneously endowing the material with antifouling properties.
authors.affiliation: Sidra Medical and Research Center , Cardiovascular Division, QCRC, Doha, Qatar.
authors.forename: Matthias
authors.initials: M
authors.lastname: Gabriel
authors.affiliation: Department of Organic Chemistry, Johannes Gutenberg-University Mainz , 55128 Mainz, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Niederer
authors.forename: Marc
authors.initials: M
authors.lastname: Becker
authors.affiliation: Sidra Medical and Research Center , Cardiovascular Division, QCRC, Doha, Qatar.
authors.forename: Christophe Michel
authors.initials: CM
authors.lastname: Raynaud
authors.forename: Christian-Friedrich
authors.initials: CF
authors.lastname: Vahl
authors.affiliation: Department of Organic Chemistry, Johannes Gutenberg-University Mainz , 55128 Mainz, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Frey
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects; D057219:Biofouling / Q000517:prevention & control*; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D011138:Polytetrafluoroethylene / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D011138:Polytetrafluoroethylene