title: Phlorizin, an Active Ingredient of Eleutherococcus senticosus, Increases Proliferative Potential of Keratinocytes with Inhibition of MiR135b and Increased Expression of Type IV Collagen.
abstract: E. senticosus extract (ESE), known as antioxidant, has diverse pharmacologic effects. It is also used as an antiaging agent for the skin and phlorizin (PZ) is identified as a main ingredient. In this study, the effects of PZ on epidermal stem cells were investigated. Cultured normal human keratinocytes and skin equivalents are used to test whether PZ affects proliferative potential of keratinocytes and how it regulates these effects. Skin equivalents (SEs) were treated with ESE and the results showed that the epidermis became slightly thickened on addition of 0.002% ESE. The staining intensity of p63 as well as proliferating cell nuclear antigen (PCNA) is increased, and integrin α6 was upregulated. Analysis of ESE confirmed that PZ is the main ingredient. When SEs were treated with PZ, similar findings were observed. In particular, the expression of integrin α6, integrin β1, and type IV collagen was increased. Levels of mRNA for type IV collagen were increased and levels of miR135b were downregulated. All these findings suggested that PZ can affect the proliferative potential of epidermal cells in part by microenvironment changes via miR135b downregulation and following increased expression of type IV collagen.
authors.affiliation: Department of Dermatology, Seoul National University Bundang Hospital, Seongnam, Gyeonggi 463-707, Republic of Korea.
authors.forename: Hye-Ryung
authors.initials: HR
authors.lastname: Choi
authors.affiliation: Department of Dermatology, Seoul National University Bundang Hospital, Seongnam, Gyeonggi 463-707, Republic of Korea.
authors.forename: Kyung-Mi
authors.initials: KM
authors.lastname: Nam
authors.affiliation: Department of Dermatology, Seoul National University Bundang Hospital, Seongnam, Gyeonggi 463-707, Republic of Korea.
authors.forename: Hyun-Sun
authors.initials: HS
authors.lastname: Lee
authors.affiliation: Department of Dermatology, Seoul National University Bundang Hospital, Seongnam, Gyeonggi 463-707, Republic of Korea.
authors.forename: Seung-Hye
authors.initials: SH
authors.lastname: Yang
authors.affiliation: Biospectrum Life Science Institute, Seongnam, Gyeonggi 462-807, Republic of Korea.
authors.forename: Young-Soo
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Department of Genetic Engineering, Sungkyunkwan University, 2066 Seobu-ro, Suwon City, Gyunggi Do 164-19, Republic of Korea.
authors.forename: Jongsung
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Medical Pharmacy, Hyogo University of Health Sciences, Kobe 650-8530, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Date
authors.affiliation: P&G Group, Kobe 658-0032, Japan.
authors.forename: Kazumi
authors.initials: K
authors.lastname: Toyama
authors.affiliation: Department of Dermatology, Seoul National University Bundang Hospital, Seongnam, Gyeonggi 463-707, Republic of Korea.
authors.forename: Kyoung-Chan
authors.initials: KC
authors.lastname: Park
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects* / Q000235:genetics; D002478:Cells, Cultured; D024141:Collagen Type IV / Q000235:genetics* / Q000378:metabolism; D028001:Eleutherococcus / Q000737:chemistry; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000502:physiology; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D010695:Phlorhizin / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
chemical_list: D000975:Antioxidants; D024141:Collagen Type IV; C000600409:MIRN135 microRNA, rat; D035683:MicroRNAs; D010695:Phlorhizin