title: Neuroprotective effect of S-allyl-l-cysteine derivatives against endoplasmic reticulum stress-induced cytotoxicity is independent of calpain inhibition.
abstract: S-allyl-l-cysteine (SAC) is known to have neuroprotective properties. We synthesized various SAC derivatives and tested their effects on endoplasmic reticulum stress-induced neurotoxicity in cultured hippocampal neurons (HPNs). Among the compounds tested, S-propyl-l-cysteine (SPC) exhibited the strongest neuroprotective activity in HPNs, followed by S-ethyl-l-cysteine (SEC) and S-methyl-l-cysteine (SMC). Unlike SAC and SMC, SPC and SEC did not have inhibitory activity on μ-calpain, suggesting that the mechanism underlying the protective activity of SPC and SEC differs from that of SAC.
authors.affiliation: Laboratory of Pharmacology, School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi-shi, Chiba 274-8555, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Imai
authors.affiliation: Laboratory of Pharmacology, School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi-shi, Chiba 274-8555, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kosuge
authors.affiliation: Laboratory of Bio-organic Chemistry, School of Pharmacy, Nihon University, 7-7-1, Narashinodai, Funabashi, Chiba 274-8555, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Saito
authors.affiliation: Laboratory of Bio-organic Chemistry, School of Pharmacy, Nihon University, 7-7-1, Narashinodai, Funabashi, Chiba 274-8555, Japan.
authors.forename: Taketo
authors.initials: T
authors.lastname: Uchiyama
authors.affiliation: Laboratory of Health Science, School of Pharmacy, Nihon University, Funabashi, Chiba, Japan.
authors.forename: Taira
authors.initials: T
authors.lastname: Wada
authors.affiliation: Laboratory of Health Science, School of Pharmacy, Nihon University, Funabashi, Chiba, Japan.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Shimba
authors.affiliation: Laboratory of Pharmacology, School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi-shi, Chiba 274-8555, Japan.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Ishige
authors.affiliation: Laboratory of Bio-organic Chemistry, School of Pharmacy, Nihon University, 7-7-1, Narashinodai, Funabashi, Chiba 274-8555, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Miyairi
authors.affiliation: Division of Biochemistry, Department of Biomedical Sciences, School of Medicine, Nihon University, 30-1 Oyaguchi-kamicho, Itabashi-ku, Tokyo 173-8610, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Makishima
authors.affiliation: Laboratory of Pharmacology, School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi-shi, Chiba 274-8555, Japan. Electronic address: ito.yoshihisa@nihon-u.ac.jp.
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Ito
mesh_terms: D000818:Animals; D002154:Calpain / Q000037:antagonists & inhibitors*; D002478:Cells, Cultured; D003545:Cysteine / Q000031:analogs & derivatives* / Q000494:pharmacology; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects* / Q000502:physiology; D006624:Hippocampus / Q000166:cytology; D009474:Neurons / Q000187:drug effects* / Q000473:pathology*; D018696:Neuroprotective Agents; D017208:Rats, Wistar
chemical_list: D018696:Neuroprotective Agents; C028742:S-propylcysteine; C065299:S-allylcysteine; C008425:S-methylcysteine; C026826:S-ethylcysteine; D002154:Calpain; D003545:Cysteine
keywords: Endoplasmic reticulum stress; Hippocampal neuron; S-allyl-l-cysteine derivatives