title: Impact of stress on Aeromonas diversity in tambaqui (Colossoma macropomum) and lectin level change towards a bacterial challenge.
abstract: Tambaqui (Colossoma macropomum) is among the most cultivated fish species in tropical countries. Stress is the main cause of disease in fish farms. The genus Aeromonas is a common causative agent of fish diseases. This work reports the identification of Aeromonas species colonizing gills of C. macropomum submitted or not to a confinement stress. We also evaluated changes in serum levels of lectins (carbohydrate-binding proteins that are components of fish immune system) in tambaqui submitted to a challenge using two isolated Aeromonas strains. Gill tissues from stressed and unstressed fishes were used to isolate Aeromonas. Then 72 Aeromonas strains were isolated, 97% being from stressed fishes. Among these, 63 were identified at species level and 6 were classified as atypical Aeromonas strains. The most prevalent species were Aeromonas bestiarum and Aeromonas caviae and their strains were used in bacterial challenges. The lectin serum levels significantly increased after 24 h of infection with A. bestiarum; however, no significant increase was found for infection with A. caviae. In conclusion, C. macropomum gills are susceptible to colonization by different Aeromonas species, mainly at confinement stressful conditions, and serum lectins may have a role in the acute immunological response towards infection by A. bestiarum.
authors.affiliation: a Departamento de Bioquímica , CCB, Universidade Federal de Pernambuco , Recife , Pernambuco , Brazil.
authors.forename: Diego S C
authors.initials: DS
authors.lastname: Marques
authors.affiliation: b Estação de Aquicultura Continental Professor Johei Koike, DEPAq , Universidade Federal Rural de Pernambuco , Recife , Pernambuco , Brazil.
authors.forename: Dijaci A
authors.initials: DA
authors.lastname: Ferreira
authors.affiliation: a Departamento de Bioquímica , CCB, Universidade Federal de Pernambuco , Recife , Pernambuco , Brazil.
authors.forename: Patrícia M G
authors.initials: PM
authors.lastname: Paiva
authors.affiliation: a Departamento de Bioquímica , CCB, Universidade Federal de Pernambuco , Recife , Pernambuco , Brazil.
authors.forename: Thiago H
authors.initials: TH
authors.lastname: Napoleão
authors.affiliation: c Departamento de Antibióticos , CCB, Universidade Federal de Pernambuco , Recife , Pernambuco , Brazil.
authors.forename: Janete M
authors.initials: JM
authors.lastname: Araújo
authors.affiliation: a Departamento de Bioquímica , CCB, Universidade Federal de Pernambuco , Recife , Pernambuco , Brazil.
authors.forename: Elba V M
authors.initials: EV
authors.lastname: Maciel Carvalho
authors.affiliation: a Departamento de Bioquímica , CCB, Universidade Federal de Pernambuco , Recife , Pernambuco , Brazil.
authors.forename: Luana C B B
authors.initials: LC
authors.lastname: Coelho
mesh_terms: D000333:Aeromonas / Q000145:classification / Q000302:isolation & purification*; D000818:Animals; D060125:Characiformes / Q000097:blood / Q000382:microbiology*; D005393:Fish Diseases / Q000097:blood / Q000382:microbiology*; D005880:Gills / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000097:blood / Q000382:microbiology* / Q000662:veterinary; D037102:Lectins / Q000097:blood*; D013312:Stress, Physiological / Q000502:physiology*
chemical_list: D037102:Lectins
keywords: Aeromonas sp.; Colossoma macropomum; bacterial challenge; fish pathogens; serum lectin