title: miR-935 promotes gastric cancer cell proliferation by targeting SOX7.
abstract: Gastric cancer is the most common cancer in the world, miRNAs have been demonstrated to play critical role in the development and progression of gastric cancer, such as miR-7, miR-217 and miR-335. Here, we found miR-935 was upregulated in gastric cancer tissues and cells. Overexpression of miR-935 promoted cell proliferation and tumorigenesis in vitro determined by MTT analysis, colony formation analysis, BrdU cell proliferation analysis and soft agar growth analysis, knockdown of miR-935 reduced these effects. Tumor suppressor sex-determining region Y-box 7 (SOX7) was the direct target of miR-935, overexpression of miR-935 inhibited SOX7 expression, but promoted the levels CCND1 and C-MYC which promotes cell proliferation and tumorigenesis, knockdown of miR-935 increased SOX7 level, and inhibited CCND1 and C-MYC expression. Synchronous knockdown of miR-935 and SOX7 promoted cell proliferation and tumorigenesis in vitro, confirming miR-935 regulated gastric cancer cell proliferation by inhibiting SOX7. In summary, we found miR-935 contributed to cell proliferation of gastric cancer through targeting SOX7.
authors.affiliation: The Second Department of Tumor Surgery, Cangzhou Central Hospital, Cangzhou, Hebei, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Yang
authors.affiliation: The Second Department of Tumor Surgery, Cangzhou Central Hospital, Cangzhou, Hebei, China.
authors.forename: Guozhong
authors.initials: G
authors.lastname: Cui
authors.affiliation: The Second Department of Tumor Surgery, Cangzhou Central Hospital, Cangzhou, Hebei, China.
authors.forename: Mingjian
authors.initials: M
authors.lastname: Ding
authors.affiliation: The Second Department of Tumor Surgery, Cangzhou Central Hospital, Cangzhou, Hebei, China.
authors.forename: Wenhua
authors.initials: W
authors.lastname: Yang
authors.affiliation: The Second Department of Tumor Surgery, Cangzhou Central Hospital, Cangzhou, Hebei, China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Liu
authors.affiliation: The Second Department of Tumor Surgery, Cangzhou Central Hospital, Cangzhou, Hebei, China.
authors.forename: Dianlu
authors.initials: D
authors.lastname: Dai
authors.affiliation: The Second Department of Tumor Surgery, Cangzhou Central Hospital, Cangzhou, Hebei, China. Electronic address: yangmeng20161770@qq.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D055760:SOXF Transcription Factors / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*; D015854:Up-Regulation / Q000235:genetics
chemical_list: C000608148:MIRN-935 microRNA, human; D035683:MicroRNAs; C484456:SOX7 protein, human; D055760:SOXF Transcription Factors
keywords: Cell proliferation; Gastric cancer; SOX7; miR-935