title: Genetic characterization of a densovirus isolated from great tit (Parus major) in China.
abstract: During a study of ornithophilous viruses in China, a new densovirus (DNV) was isolated from the lung tissue of Parus major (PmDNV-JL). The complete genome of PmDNV-JL was cloned and sequenced. Five open reading frames (ORFs) were identified in the 5166nt sequence, on the basis of deduced amino acids. It was further shown that this virus caused cytopathic effects (CPE) in Feline kidney cells. The NS1 gene sequence of PmDNV-JL shares 70-99% nucleotide sequence identity with isolates of the Blattella germanica densovirus (BgDNV) and BgDNV-like virus. Phylogenetic analysis indicated that the predicted amino acid sequences of capsid (VP) and non-structural domain (NS1) of PmDNV-JL clustered with the BgDNV and were similar to BgDNV-HB within the genus Densovirus.
authors.affiliation: College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal probiotics, Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Wen-Tao
authors.initials: WT
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal probiotics, Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Shi
authors.affiliation: College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal probiotics, Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Yan-Long
authors.initials: YL
authors.lastname: Jiang
authors.affiliation: College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal probiotics, Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.affiliation: College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal probiotics, Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Hong-Liang
authors.initials: HL
authors.lastname: Chen
authors.affiliation: College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal probiotics, Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Ke-Yan
authors.initials: KY
authors.lastname: Huang
authors.affiliation: College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal probiotics, Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China. Electronic address: yangguilian@jlau.edu.cn.
authors.forename: Gui-Lian
authors.initials: GL
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal probiotics, Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China. Electronic address: wangchunfeng@jlau.edu.cn.
authors.forename: Chun-Feng
authors.initials: CF
authors.lastname: Wang
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000453:epidemiology* / Q000635:transmission / Q000821:virology; D036022:Capsid Proteins / Q000235:genetics; D002415:Cats; D002681:China / Q000453:epidemiology; D004279:DNA, Viral / Q000235:genetics; D017990:Densovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004847:Epithelial Cells / Q000821:virology; D016679:Genome, Viral; D007668:Kidney / Q000821:virology; D008168:Lung / Q000821:virology; D016366:Open Reading Frames; D010322:Parvoviridae Infections / Q000453:epidemiology / Q000635:transmission / Q000662:veterinary* / Q000821:virology; D046109:Passeriformes / Q000821:virology*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017361:Viral Nonstructural Proteins / Q000235:genetics
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral; D017361:Viral Nonstructural Proteins
keywords: Densovirus; Lung tissue; Parus major; Sequence analysis