title: CHD3 chromatin-remodeling factor PICKLE regulates floral transition partially via modulating LEAFY expression at the chromatin level in Arabidopsis.
abstract: PICKLE (PKL), a putative CHD3 chromatin remodeling factor, has been suggested to be involved in multiple processes in Arabidopsis. Here, we confirmed the late-flowering phenotype caused by pkl mutation with pkl mutants in two different ecotypes, and investigated the possible mechanisms that account for PKL regulation of flowering time. Quantitative RT-PCR and RNA-seq assays showed that expression of the LEAFY gene (LFY) and a number of LFY-regulated floral homeotic genes were down-regulated in seedlings of the pkl mutants. As predicted, overexpression of LFY restored normal flowering time of pkl mutants. Our results suggest that PKL may be involved in regulating flowering time via LFY expression. To uncover the underlying mechanism, ChIP-PCR using anti-PKL was performed on materials from three developmental stages of seedlings. Our results showed that PKL associated with the genomic sequences of LFY, particularly at 10-day and 25-day after germination. We also showed that loss of PKL affected H3K27me3 level at the promoter of LFY. Taken together, our data suggest that transcriptional regulation of LFY at the chromatin level by PKL may at least partially account for the late-flowering phenotype of pkl mutants.
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, 100871, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Fu
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, 100871, China.
authors.forename: Chaonan
authors.initials: C
authors.lastname: Li
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, 100871, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, 100871, China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, 100871, China.
authors.forename: Hang
authors.initials: H
authors.lastname: He
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, 100871, China. lmfan@pku.edu.cn.
authors.forename: Liu-Min
authors.initials: LM
authors.lastname: Fan
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000502:physiology*; D002843:Chromatin / Q000235:genetics*; D004265:DNA Helicases / Q000235:genetics / Q000502:physiology*; D035264:Flowers / Q000254:growth & development*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D009154:Mutation; D060888:Real-Time Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; D002843:Chromatin; C074747:LFY protein, Arabidopsis; D014157:Transcription Factors; C402442:AT2G25170 protein, Arabidopsis; D004265:DNA Helicases
keywords: Arabidopsis; LEAFY; PICKLE; chromatin-remodeling factor; floral transition