title: Cotton Leaf Curl Multan Betasatellite DNA as a Tool to Deliver and Express the Human B-Cell Lymphoma 2 (Bcl-2) Gene in Plants.
abstract: The betasatellite DNA associated with Cotton leaf curl Multan virus (CLCuMB) contains a single complementary-sense ORF, βC1, which is a pathogenicity determinant. CLCuMB was able to replicate in plants in the presence of diverse helper geminiviruses, including Tomato leaf curl virus-Australia (TLCV-Au), Iranian isolate of Tomato yellow leaf curl virus (TYLCV-[Ab]), and Beet curly top virus (BCTV-Svr), and can be used as a plant gene delivery vector. To test the hypothesis that CLCuMB has the potential to act as an animal gene delivery vector, a specific insertion construct was produced by the introduction of a human B-cell lymphoma 2 (Bcl-2) cDNA into a mutant DNA of CLCuMB in which the βC1 was deleted (β∆C1). The recombinant βΔC1-Bcl-2 construct was successfully replicated in tomato and tobacco plants in the presence of TLCV-Au, BCTV-Svr and TYLCV-[Ab]. Real-time PCR and Western blot analyses of plants containing the replicative forms of recombinant βΔC1-Bcl-2 DNA showed that Bcl-2 gene was expressed in an acceptable level in these plants, indicating that β∆C1 can be used as a tool to deliver and express animal genes in plants. This CLCuMB-based system, having its own promoter activity, offers the possibility of production of animal recombinant proteins in plants.
authors.affiliation: Institute of Biotechnology, Shiraz University, Shiraz, Iran.
authors.forename: Sara
authors.initials: S
authors.lastname: Kharazmi
authors.affiliation: Institute of Biotechnology, Shiraz University, Shiraz, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Ataie Kachoie
authors.affiliation: Plant Virology Research Center, College of Agriculture, Shiraz University, Shiraz, Iran. akbar_behjatnia@hotmail.com.
authors.forename: Seyed Ali Akbar
authors.initials: SA
authors.lastname: Behjatnia
mesh_terms: D004279:DNA, Viral / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D010942:Plant Viruses / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D014162:Transfection
chemical_list: D004279:DNA, Viral; D019253:Proto-Oncogene Proteins c-bcl-2
keywords: Betasatellite; CLCuMB; Delivery vector; Geminivirus; Gene expression; Human Bcl-2