title: Encapsulation of gallic acid/cyclodextrin inclusion complex in electrospun polylactic acid nanofibers: Release behavior and antioxidant activity of gallic acid.
abstract: Cyclodextrin-inclusion complexes (CD-ICs) possess great prominence in food and pharmaceutical industries due to their enhanced ability for stabilization of active compounds during processing, storage and usage. Here, CD-IC of gallic acid (GA) with hydroxypropyl-beta-cyclodextrin (GA/HPβCD-IC) was prepared and then incorporated into polylactic acid (PLA) nanofibers (PLA/GA/HPβCD-IC-NF) using electrospinning technique to observe the effect of CD-ICs in the release behavior of GA into three different mediums (water, 10% ethanol and 95% ethanol). The GA incorporated PLA nanofibers (PLA/GA-NFs) were served as control. Phase solubility studies showed an enhanced solubility of GA with increasing amount of HPβCD. The detailed characterization techniques (XRD, TGA and (1)H-NMR) confirmed the formation of inclusion complex between GA and HPβCD. Computational modeling studies indicated that the GA made an efficient complex with HPβCD at 1:1 either in vacuum or aqueous system. SEM images revealed the bead-free and uniform morphology of PLA/GA/HPβCD-IC-NF. The release studies of GA from PLA/GA/HPβCD-IC-NF and PLA/GA-NF were carried out in water, 10% ethanol and 95% ethanol, and the findings revealed that PLA/GA/HPβCD-IC-NF has released much more amount of GA in water and 10% ethanol system when compared to PLA/GA-NF. In addition, GA was released slowly from PLA/GA/HPβCD-IC-NF into 95% ethanol when compared to PLA/GA-NF. It was also observed that electrospinning process had no negative effect on the antioxidant activity of GA when GA was incorporated in PLA nanofibers.
authors.affiliation: Institute of Materials Science & Nanotechnology, Bilkent University, Ankara 06800, Turkey; UNAM-National Nanotechnology Research Center, Bilkent University, Ankara 06800, Turkey.
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Aytac
authors.affiliation: UNAM-National Nanotechnology Research Center, Bilkent University, Ankara 06800, Turkey; Department of Engineering Physics, Istanbul Medeniyet University, Göztepe 34700, Istanbul, Turkey.
authors.forename: Semran Ipek
authors.initials: SI
authors.lastname: Kusku
authors.affiliation: Institute of Materials Science & Nanotechnology, Bilkent University, Ankara 06800, Turkey; UNAM-National Nanotechnology Research Center, Bilkent University, Ankara 06800, Turkey.
authors.forename: Engin
authors.initials: E
authors.lastname: Durgun
authors.affiliation: Institute of Materials Science & Nanotechnology, Bilkent University, Ankara 06800, Turkey; UNAM-National Nanotechnology Research Center, Bilkent University, Ankara 06800, Turkey. Electronic address: tamer@unam.bilkent.edu.tr.
authors.forename: Tamer
authors.initials: T
authors.lastname: Uyar
mesh_terms: D000073738:2-Hydroxypropyl-beta-cyclodextrin; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism; D005707:Gallic Acid / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D012995:Solubility; D013818:Thermogravimetry; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D011091:Polyesters; D047392:beta-Cyclodextrins; D014867:Water; D000073738:2-Hydroxypropyl-beta-cyclodextrin; C033616:poly(lactide); D005707:Gallic Acid
keywords: Antioxidant activity; Electrospinning; Gallic acid; Hydroxypropyl-beta-cyclodextrin; Polylactic acid; Release