title: Affinity purification lipase from wheat germ: comparison of hydrophobic and metal chelation effect.
abstract: Cryogels are used quite a lot nowadays for adsorption studies as synthetic adsorbents. In this study, lipase enzyme (obtained from Candida cylindracea) adsorption capacity of poly(2-hydroxyethyl methacrylate-N-methacryloyl-L-tryptophan), poly(HEMA-MATrp), and Cu(II) ions immobilized poly(HEMA-MATrp), poly(HEMA-MATrp)-Cu(II), cryogel membranes were synthesized to determine and compare the adsorption behavior of lipase enzyme. In this regard, the effect of pH, interaction time, lipase initial concentration, temperature and ionic strength on the adsorption capacity of these membranes was investigated. Maximum lipase enzyme adsorption capacities of poly(HEMA-MATrp) and poly(HEMA-MATrp)-Cu(II) cryogel membranes were determined as 166.4 mg/g and 196.4 mg/g, respectively.
authors.affiliation: a Scientific Technical Research and Application Center , Hitit University , Çorum , Turkey.
authors.forename: Kazım
authors.initials: K
authors.lastname: Köse
authors.affiliation: b Department of Chemistry, Faculty of Science and Literature , Hitit University , Çorum , Turkey.
authors.forename: Kadir
authors.initials: K
authors.lastname: Erol
authors.affiliation: b Department of Chemistry, Faculty of Science and Literature , Hitit University , Çorum , Turkey.
authors.forename: Dursun
authors.initials: D
authors.lastname: Ali Köse
authors.affiliation: c Department of Molecular Biology and Genetics, Faculty of Science and Literature , Hitit University , Çorum , Turkey.
authors.forename: Emre
authors.initials: E
authors.lastname: Evcı
authors.affiliation: d Department of Chemistry , Hacettepe University, Faculty of Science , Ankara , Turkey.
authors.forename: Lokman
authors.initials: L
authors.lastname: Uzun
mesh_terms: D000327:Adsorption; D002175:Candida / Q000737:chemistry* / Q000201:enzymology; D002413:Cations, Divalent; D002614:Chelating Agents / Q000737:chemistry; D002846:Chromatography, Affinity / Q000379:methods; D003300:Copper / Q000737:chemistry; D059985:Cryogels / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000302:isolation & purification*; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D008049:Lipase / Q000737:chemistry / Q000302:isolation & purification*; D009994:Osmolar Concentration; D011109:Polymethacrylic Acids / Q000737:chemistry*; D013696:Temperature; D014908:Triticum / Q000382:microbiology; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D002413:Cations, Divalent; D002614:Chelating Agents; D059985:Cryogels; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D011109:Polymethacrylic Acids; C579335:poly(hydroxyethyl methacrylate-N-methacryloyltyroptophan methylester); D003300:Copper; D014364:Tryptophan; D008049:Lipase
keywords: Adsorption; cryogel; enzyme; lipase; membrane