title: Homozygous SLC4A11 mutation in a large Irish CHED2 pedigree.
abstract: Congenital hereditary endothelial dystrophy (CHED) is a genetic disorder of corneal endothelial cells resulting in corneal clouding and visual impairment. Autosomal dominant (CHED1) and autosomal recessive (CHED2) forms have been reported and map to distinct loci on chromosome 20. CHED2 is caused by mutations in the SLC4A11 gene which encodes a membrane transporter protein.
  
  
  
  Members of a large CHED2 family were recruited for clinical and genetic studies. Genomic DNA was sequenced for the exons and intron-exon boundaries of the SLC4A11 gene.
  
  
  
  Twelve family members were recruited, of which eight were diagnosed with CHED. A homozygous SLC4A11 mutation (Leu843Pro) was detected in the eight patients; a single copy of the mutation was present in three unaffected carriers.
  
  
  
  A missense SLC4A11 mutation (Leu843Pro) is responsible for CHED2 in this family; this is the first report of this mutation in a homozygous state.
authors.affiliation: a Department of Pathology , University College Cork , Cork , Ireland.
authors.forename: Collette K
authors.initials: CK
authors.lastname: Hand
authors.affiliation: b Department of Ophthalmology , Royal Victoria Eye and Ear Hospital , Dublin , Ireland.
authors.forename: Mairide
authors.initials: M
authors.lastname: McGuire
authors.affiliation: a Department of Pathology , University College Cork , Cork , Ireland.
authors.forename: Nollaig A
authors.initials: NA
authors.lastname: Parfrey
authors.affiliation: b Department of Ophthalmology , Royal Victoria Eye and Ear Hospital , Dublin , Ireland.|c Department of Ophthalmology , Royal College of Surgeons in Ireland , Dublin , Ireland.
authors.forename: Conor C
authors.initials: CC
authors.lastname: Murphy
mesh_terms: D000293:Adolescent; D000328:Adult; D027321:Anion Transport Proteins / Q000235:genetics*; D017920:Antiporters / Q000235:genetics*; D003241:Consanguinity; D003317:Corneal Dystrophies, Hereditary / Q000175:diagnosis / Q000235:genetics*; D004252:DNA Mutational Analysis; D005091:Exons / Q000235:genetics; D005260:Female; D006720:Homozygote; D006801:Humans; D007438:Introns / Q000235:genetics; D007494:Ireland / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D010375:Pedigree; D055815:Young Adult
chemical_list: D027321:Anion Transport Proteins; D017920:Antiporters; C425809:SLC4A11 protein, human
keywords: Congenital hereditary endothelial dystrophy; Fuchs endothelial dystrophy; Harboyan syndrome; SLC4A11 gene; corneal dystrophy and perceptive deafness