title: Hydrogen sulfide modulates sub-cellular susceptibility to oxidative stress induced by myocardial ischemic reperfusion injury.
abstract: In this study, we compared the impact of H2S pre (HIPC) and post-conditioning (HPOC) on oxidative stress, the prime reason for myocardial ischemia reperfusion injury (I/R), in different compartments of the myocardium, such as the mitochondria beside its subpopulations (interfibrillar (IFM) and subsarcolemmal (SSM) mitochondria) and microsomal fractions in I/R injured rat heart. The results demonstrated that compared to I/R rat heart, HIPC and HPOC treated hearts shows reduced myocardial injury, enhanced antioxidant enzyme activities and reduced the level of TBARS in different cellular compartments. The extent of recovery (measured by TBARS and GSH levels) in subcellular fractions, were in the following descending order: microsome > SSM > IFM in both HIPC and HPOC. In summary, oxidative stress mediated mitochondrial dysfunction, one of the primary causes for I/R injury, was partly recovered by HIPC and HPOC treatment, with significant improvement in SSM fraction compared to the IFM.
authors.affiliation: School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamilnadu, India.
authors.forename: Shakila Banu
authors.initials: SB
authors.lastname: Ansari
authors.affiliation: School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamilnadu, India. Electronic address: ginokurian@hotmail.com.
authors.forename: Gino A
authors.initials: GA
authors.lastname: Kurian
mesh_terms: D000818:Animals; D002316:Cardiotonic Agents / Q000627:therapeutic use*; D064426:Gasotransmitters / Q000627:therapeutic use*; D006862:Hydrogen Sulfide / Q000627:therapeutic use*; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002316:Cardiotonic Agents; D064426:Gasotransmitters; D006862:Hydrogen Sulfide
keywords: Hydrogen sulfide; Interfibrillar mitochondria; Ischemia-reperfusion; Oxidative stress; Subsarcolemmal mitochondria