title: Changes in iTRAQ-Based Proteomic Profiling of the Cladoceran Daphnia magna Exposed to Microcystin-Producing and Microcystin-Free Microcystis aeruginosa.
abstract: Global warming and increased nutrient fluxes cause cyanobacterial blooms in freshwater ecosystems. These phenomena have increased the concern for human health and ecosystem services. The mass occurrences of toxic cyanobacteria strongly affect freshwater zooplankton communities, especially the unselective filter feeder Daphnia. However, the molecular mechanisms of cyanobacterial toxicity remain poorly understood. This study is the first to combine the established body growth rate (BGR), which is an indicator of life-history fitness, with differential peptide labeling (iTRAQ)-based proteomics in Daphnia magna influenced by microcystin-producing (MP) and microcystin-free (MF) Microcystis aeruginosa. A significant decrease in BGR was detected when D. magna was exposed to MP or MF M. aeruginosa. Conducting iTRAQ proteomic analyses, we successfully identified and quantified 211 proteins with significant changes in expression. A cluster of orthologous groups revealed that M. aeruginosa-affected differential proteins were strongly associated with lipid, carbohydrate, amino acid, and energy metabolism. These parameters could potentially explain the reduced fitness based on the cost of the substance metabolism.
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Biological Sciences, Nanjing Normal University , 1 Wenyuan Road, Nanjing 210023, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Lyu
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Biological Sciences, Nanjing Normal University , 1 Wenyuan Road, Nanjing 210023, China.
authors.forename: Qingguo
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Biological Sciences, Nanjing Normal University , 1 Wenyuan Road, Nanjing 210023, China.
authors.forename: Xuexia
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Biological Sciences, Nanjing Normal University , 1 Wenyuan Road, Nanjing 210023, China.
authors.forename: Daoxin
authors.initials: D
authors.lastname: Dai
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Biological Sciences, Nanjing Normal University , 1 Wenyuan Road, Nanjing 210023, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Biological Sciences, Nanjing Normal University , 1 Wenyuan Road, Nanjing 210023, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Biological Sciences, Nanjing Normal University , 1 Wenyuan Road, Nanjing 210023, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000818:Animals; D000458:Cyanobacteria / Q000378:metabolism; D003621:Daphnia / Q000378:metabolism; D052998:Microcystins / Q000378:metabolism*; D046931:Microcystis / Q000378:metabolism*; D040901:Proteomics
chemical_list: D052998:Microcystins