title: Effects of intracerebral hemorrhage on 5-hydroxymethylcytosine modification in mouse brains.
abstract: The past decade has resulted in an increase in the knowledge of molecular mechanisms underlying brain injury induced by intracerebral hemorrhage (ICH). Recent advances have provided a link between epigenetic modification and the regulation of gene expression. 5-hydroxymethylcytosine (5hmC) converted from 5-methylcytosine by the ten-eleven translocation (TET) family of proteins has emerged as a new epigenetic modification. While the dynamics of 5hmC during cerebral ischemia have recently been reported, whether 5hmC is involved in ICH remains unexplored. In this study, we investigated the effects of ICH on DNA hydroxymethylation. We showed that the global level of 5hmC rapidly decreased as early as 24 hours after ICH and persisted until 72 hours. Furthermore, the level of 5hmC in the CpG-rich regions of Akt2, Pdpk1 and Vegf genes was significantly decreased with a minimum level observed at 48 hours or 72 hours. Decreased 5hmC was observed in parallel with an increase in 5-methylcytosine over this time course, and mRNA levels of Akt2, Pdpk1 and Vegf were downregulated upon ICH injury. Finally, Tet1, Tet2 and Tet3 mRNA levels were dramatically decreased in the ICH brain. Our study for the first time established the correlation between DNA hydroxymethylation and ICH injury. Further investigations should examine whether 5hmC modification could be a therapeutic target for the treatment of ICH injury.
authors.affiliation: Department of Neurology, Huashan Hospital of Fudan University, Shanghai, People's Republic of China.
authors.forename: Yilin
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Neurology, Huashan Hospital of Fudan University, Shanghai, People's Republic of China.
authors.forename: Sha
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Neurosurgery, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, Japan; Department of Psychiatry, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Asakawa
authors.affiliation: Department of Neurology, Huashan Hospital of Fudan University, Shanghai, People's Republic of China.
authors.forename: Yunhe
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Department of Neurology, Huashan Hospital of Fudan University, Shanghai, People's Republic of China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Han
authors.affiliation: Institute of Neurology, Huashan Hospital of Fudan University, Shanghai, People's Republic of China.
authors.forename: Baoguo
authors.initials: B
authors.lastname: Xiao
authors.affiliation: Department of Neurology, Huashan Hospital of Fudan University, Shanghai, People's Republic of China; Institute of Neurology, Huashan Hospital of Fudan University, Shanghai, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Dong
authors.affiliation: Department of Neurology, Huashan Hospital of Fudan University, Shanghai, People's Republic of China; Institute of Neurology, Huashan Hospital of Fudan University, Shanghai, People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
keywords: DNA hydroxymethylation; TET protein; cerebral edema; stroke