title: Maintenance of Dermal Papilla Cells by Wnt-10b In Vitro.
abstract: Dermal papilla cells (DPCs) are associated with development of hair follicles (HFs) and regulation of the hair cycle. However, primary DPCs are known to lose their ability to induce HFs after culture in standard media for fibroblasts. We examined a new culture condition for DPCs including addition of Wnt-10b, which promoted proliferation and maintained their HF induction ability for more than ten passages. These results suggest that Wnt-10b plays a pivotal role in proliferation and maintenance of DPCs in vitro.
authors.affiliation: Department of Pathogen, Infection and Immunity, Nara Medical University, 840 Shijo-Cho, Kashihara, Nara, 634-8521, Japan. oujix@naramed-u.ac.jp.
authors.forename: Yukiteru
authors.initials: Y
authors.lastname: Ouji
authors.affiliation: Department of Pathogen, Infection and Immunity, Nara Medical University, 840 Shijo-Cho, Kashihara, Nara, 634-8521, Japan. myoshika@naramed-u.ac.jp.
authors.forename: Masahide
authors.initials: M
authors.lastname: Yoshikawa
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation / Q000235:genetics; D020405:Dermis / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology; D018859:Hair Follicle / Q000166:cytology*; D051379:Mice; D051153:Wnt Proteins / Q000235:genetics*
chemical_list: D051153:Wnt Proteins; C102068:Wnt10b protein, mouse
keywords: Cell culture; Cell proliferation; Dermal papilla; Wnt signaling; Wnt-10b