title: Modeling single cell antibody excretion on a biosensor.
abstract: We simulated, using Comsol Multiphysics, the excretion of antibodies by single hybridoma cells and their subsequent binding on a surface plasmon resonance imaging (SPRi) sensor. The purpose was to confirm that SPRi is suitable to accurately quantify antibody (anti-EpCAM) excretion. The model showed that antibody loss by diffusion away from the sensor was less than 1%. Unexpectedly, more than 99% of the excreted antibodies were captured on the sensor. These data prove the remarkable phenomenon that the SPRi output of cellular antibody excretion and its subsequent binding, performed under the conditions described here, is directly usable for quantification of single cell antibody production rates.
authors.affiliation: Medical Cell Biophysics Group, MIRA Institute, Faculty of Science and Technology, University of Twente, 7500 AE Enschede, The Netherlands. Electronic address: i.stojanovic@utwente.nl.
authors.forename: Ivan
authors.initials: I
authors.lastname: Stojanović
authors.affiliation: Medical Cell Biophysics Group, MIRA Institute, Faculty of Science and Technology, University of Twente, 7500 AE Enschede, The Netherlands.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Baumgartner
authors.affiliation: Medical Cell Biophysics Group, MIRA Institute, Faculty of Science and Technology, University of Twente, 7500 AE Enschede, The Netherlands; IBIS Technologies, 7521 PR Enschede, The Netherlands.
authors.forename: Thomas J G
authors.initials: TJ
authors.lastname: van der Velden
authors.affiliation: Medical Cell Biophysics Group, MIRA Institute, Faculty of Science and Technology, University of Twente, 7500 AE Enschede, The Netherlands.
authors.forename: Leon W M M
authors.initials: LW
authors.lastname: Terstappen
authors.affiliation: Medical Cell Biophysics Group, MIRA Institute, Faculty of Science and Technology, University of Twente, 7500 AE Enschede, The Netherlands; IBIS Technologies, 7521 PR Enschede, The Netherlands.
authors.forename: Richard B M
authors.initials: RB
authors.lastname: Schasfoort
mesh_terms: D000906:Antibodies / Q000276:immunology / Q000378:metabolism*; D000917:Antibody Formation; D004058:Diffusion; D000071858:Epithelial Cell Adhesion Molecule / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D020349:Surface Plasmon Resonance
chemical_list: D000906:Antibodies; D000071858:Epithelial Cell Adhesion Molecule
keywords: EpCAM; Hybridoma cells; Quantification; SPRi; Simulation