title: Evaluation of 2,4-dichlorophenol exposure of Japanese medaka, Oryzias latipes, using a metabolomics approach.
abstract: In this study, the metabolic effects of waterborne exposure of medaka (Oryzias latipes) to nominal concentrations of 20 (L group) and 2000 μg/L (H group) 2,4-dichlorophenol (DCP) were examined using a gas chromatography/mass spectroscopy (GC/MS) metabolomics approach. A principal component analysis (PCA) separated the L, H, and control groups along PC1 to explain the toxic effects of DCP at 24 h of exposure. Furthermore, the L and H groups were separated along PC1 at 96 h on the PCA score plots. These results suggest that the effects of DCP depended on exposure concentration and time. Changes in tricarboxylic cycle metabolites suggested that fish exposed to 2,4-DCP require more energy to metabolize and eliminate DCP, particularly at 96 h of exposure. A time-dependent response in the fish exposed to DCP was observed in the GC/MS data, suggesting that the higher DCP concentration had greater effects at 24 h than those observed in response to the lower concentration. In addition, several essential amino acids (arginine, histidine, lysine, isoleucine, leucine, methionine, phenylalanine, threonine, tryptophan, and valine) decreased after DCP exposure in the H group, and starvation condition and high concentration exposure of DCP could consume excess energy from amino acids.
authors.affiliation: Education and Research Center for Marine Resources and Environment, Faculty of Fisheries, Kagoshima University, 4-50-20 Shimoarata, Kagoshima, 890-0056, Japan. kokushi@fish.kagoshima-u.ac.jp.
authors.forename: Emiko
authors.initials: E
authors.lastname: Kokushi
authors.affiliation: Education and Research Center for Marine Resources and Environment, Faculty of Fisheries, Kagoshima University, 4-50-20 Shimoarata, Kagoshima, 890-0056, Japan.
authors.forename: Aoi
authors.initials: A
authors.lastname: Shintoyo
authors.affiliation: Education and Research Center for Marine Resources and Environment, Faculty of Fisheries, Kagoshima University, 4-50-20 Shimoarata, Kagoshima, 890-0056, Japan.
authors.forename: Jiro
authors.initials: J
authors.lastname: Koyama
authors.affiliation: Education and Research Center for Marine Resources and Environment, Faculty of Fisheries, Kagoshima University, 4-50-20 Shimoarata, Kagoshima, 890-0056, Japan.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Uno
mesh_terms: D000818:Animals; D002733:Chlorophenols / Q000633:toxicity*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D055432:Metabolomics; D009990:Oryzias / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D002733:Chlorophenols; D014874:Water Pollutants, Chemical; C004762:2,4-dichlorophenol
keywords: Dichlorophenol; Gluconeogenesis; Medaka; Metabolomics; Proteolysis; TCA cycle