title: Assessment of physicochemical parameters and prevalence of virulent and multiple-antibiotic-resistant Escherichia coli in treated effluent of two wastewater treatment plants and receiving aquatic milieu in Durban, South Africa.
abstract: The poor operational status of some wastewater treatment plants often result in the discharge of inadequately treated effluent into receiving surface waters. This is of significant public health concern as there are many informal settlement dwellers (ISDs) that rely on these surface waters for their domestic use. This study investigated the treatment efficiency of two independent wastewater treatment plants (WWTPs) in Durban, South Africa and determined the impact of treated effluent discharge on the physicochemical and microbial quality of the receiving water bodies over a 6-month period. Presumptive Escherichia coli isolates were identified using biochemical tests and detection of the mdh gene via PCR. Six major virulence genes namely eae, hly, fliC, stx1, stx2, and rfbE were also detected via PCR while antibiotic resistance profiles of the isolates were determined using Kirby-Bauer disc diffusion assay. The physicochemical parameters of the wastewater samples ranged variously between 9 and 313.33 mg/L, 1.52 and 76.43 NTUs, and 6.30 and 7.87 for COD, turbidity, and pH respectively, while the E. coli counts ranged between 0 and 31.2 × 10(3) CFU/ml. Of the 200 selected E. coli isolates, the hly gene was found in 28 %, fliC in 20 %, stx2 in 17 %, eae in 14 %, with stx1 and rfbE in only 4 % of the isolates. Notable resistance was observed toward trimethoprim (97 %), tetracycline (56 %), and ampicillin (52.5 %). These results further highlight the poor operational status of these WWTPs and outline the need for improved water quality monitoring and enforcement of stringent guidelines.
authors.affiliation: Discipline of Microbiology, School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal (Westville Campus), Private Bag X54001, Durban, 4000, Republic of South Africa.
authors.forename: Leanne
authors.initials: L
authors.lastname: Pillay
authors.affiliation: Discipline of Microbiology, School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal (Westville Campus), Private Bag X54001, Durban, 4000, Republic of South Africa. olanirana@ukzn.ac.za.
authors.forename: Ademola O
authors.initials: AO
authors.lastname: Olaniran
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004784:Environmental Monitoring / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity; D015995:Prevalence; D013019:South Africa; D014865:Waste Disposal, Fluid; D062065:Wastewater / Q000737:chemistry*; D060753:Water Quality
chemical_list: D000900:Anti-Bacterial Agents; D062065:Wastewater
keywords: Antibiotic resistance; Escherichia coli; Physicochemical parameters; Virulence genes; Wastewater effluents