title: MOLECULAR CHARACTERIZATION OF THE LECTIN, BRYOHEALIN, INVOLVED IN PROTOPLAST REGENERATION OF THE MARINE ALGA BRYOPSIS PLUMOSA (CHLOROPHYTA)(1).
abstract: When a coenocytic cell of the green alga Bryopsis plumosa (Hudson) C. Agardh was cut open and the cell contents expelled, the cell organelles agglutinated rapidly in seawater to form protoplasts. This process was mediated by a lectin, Bryohealin. The full sequence of the cDNA encoding Bryohealin was obtained, which consisted of 1,101 base pairs (bp), with 24 bp of 5' untranslated region (UTR) and 201 bp of 3' UTR. It had an open reading frame (ORF) of 771 bp encoding 257 amino acid residues. A signal peptide consisted of 22 amino acids presented before the start codon of Bryohealin, indicating that this lectin was a vacuolar (storage) protein. The C-terminal sequence of Bryohealin was composed of antibiotic domains, suggesting that this lectin could perform two functions: (i) aggregation of cell organelles in seawater and (ii) protection from bacterial contamination for successful protoplast regeneration. The BLAST search result showed that Bryohealin had little sequence homology with any known plant lectins, but rather resembled animal lectins with fucolectin domains. The expression of recombinant Bryohealin (rBryohealin) was obtained in the Escherichia coli system.
authors.affiliation: Department of Biology, Kongju National University, Kongju, 314-701, KoreaDepartment of Chemistry, Kongju National University, Kongju, 314-701, KoreaDepartment of Biology, Kongju National University, Kongju, 314-701, Korea.
authors.forename: Kang Sup
authors.initials: KS
authors.lastname: Yoon
authors.affiliation: Department of Biology, Kongju National University, Kongju, 314-701, KoreaDepartment of Chemistry, Kongju National University, Kongju, 314-701, KoreaDepartment of Biology, Kongju National University, Kongju, 314-701, Korea.
authors.forename: Key Pyoung
authors.initials: KP
authors.lastname: Lee
authors.affiliation: Department of Biology, Kongju National University, Kongju, 314-701, KoreaDepartment of Chemistry, Kongju National University, Kongju, 314-701, KoreaDepartment of Biology, Kongju National University, Kongju, 314-701, Korea.
authors.forename: Tatyana A
authors.initials: TA
authors.lastname: Klochkova
authors.affiliation: Department of Biology, Kongju National University, Kongju, 314-701, KoreaDepartment of Chemistry, Kongju National University, Kongju, 314-701, KoreaDepartment of Biology, Kongju National University, Kongju, 314-701, Korea.
authors.forename: Gwang Hoon
authors.initials: GH
authors.lastname: Kim
keywords: Bryohealin; Bryopsis plumosa; green algae; lectin; protoplast formation; recombinant