title: Caveolin-1 is involved in high glucose accelerated human glomerular mesangial cell senescence.
abstract: OBJECTIVE
  We demonstrated the role of caveolin-1 involved in high glucose (HG)-induced glomerular mesangial cells (GMCs) senescence.
  
  
  METHODS
  HG was used to stimulate GMCs. The telomere lengths were analyzed by Southern blot. β-Galactosidase staining was determined. The expressions of caveolin-1 and P53 proteins were determined by Western blot.
  
  
  RESULTS
  Treatment with high concentrations of glucose induced GMC senescence accompanied by shortened telomere length and increase of β-galactosidase staining as well as P53 protein, which was abrogated after application of caveolin-1-siRNA.
  
  
  CONCLUSIONS
  This study proved that HG induced cell senescence in GMCs. The caveolin-1 is involved in HG-induced mesangial cell senescence, and blocking caveolin-1 significantly reduced cell senescence. The effect of caveolin-1 is mediated by P53 pathway.
authors.affiliation: Department of Rheumatology, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Feng
authors.affiliation: Department of Rheumatology, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.affiliation: Department of Physiology, Liaoning Medical University, Jinzhou, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Li
mesh_terms: D051242:Caveolin 1 / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects*; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D005947:Glucose / Q000633:toxicity*; D006801:Humans; D050527:Mesangial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D034622:RNA Interference; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D059506:Telomere Shortening / Q000187:drug effects; D013997:Time Factors; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: C495540:CAV1 protein, human; C493956:CDKN1A protein, human; D051242:Caveolin 1; D050759:Cyclin-Dependent Kinase Inhibitor p21; D017382:Reactive Oxygen Species; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D001616:beta-Galactosidase; D005947:Glucose
keywords: Aging; Caveolin-1; Mesangial cells