title: An in vitro study of alkaline phosphatase sensitivity to mixture of aflatoxin B1 and fumonisin B1 in the hepatopancreas of coastal lagoon wild and farmed shrimp Litopenaeus vannamei.
abstract: This study aimed to establish the combined effect of aflatoxin B1 (AFB1) and fumonisin B1 (FB1) on wild Litopenaeus vannamei hepatopancreas alkaline phosphatase (AP) activity compared with that of farmed shrimp. AP activity in hepatopancreas extract was confirmed by several specific inhibitor assays. AP activity of wild shrimp was higher than that of farmed shrimp (p < 0.05). However, AP activity from both wild and farmed shrimp was inhibited when incubated with AFB1 and FB1. The greatest inhibition occurred when AP was incubated with a mixture of AFB1 and FB1. The IC50 for AFB1 on AP activity of wild and farmed shrimp hepatopancreases was 0.790 and 0.398 μg/mL, respectively. The IC50 of FB1 was 0.87 μg/mL for wild shrimp and 0.69 μg/mL for farmed shrimp. These results suggest that, at the mycotoxins concentrations used in the study, AP from farmed L. vannamei was sensitive to the presence of both mycotoxins; however, AP is more sensitive to the combination of AFB1 + FB1 suggesting a possible synergistic or potentiating inhibitory effect.
authors.affiliation: Department of Research and Postgraduate in Foods, University of Sonora, Blvd. Luis Encinas y Rosales s/n, Col. Centro, Apdo. Postal 1658, 83000, Hermosillo, Sonora, Mexico.
authors.forename: Jesús A
authors.initials: JA
authors.lastname: Pérez-Acosta
authors.affiliation: Department of Research and Postgraduate in Foods, University of Sonora, Blvd. Luis Encinas y Rosales s/n, Col. Centro, Apdo. Postal 1658, 83000, Hermosillo, Sonora, Mexico.
authors.forename: Armando
authors.initials: A
authors.lastname: Burgos-Hernandez
authors.affiliation: Department of Chemical and Biological Sciences, University of Sonora, Blvd. Luis Encinas y Rosales s/n, Col. Centro, 83000, Hermosillo, Sonora, Mexico.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Velázquez-Contreras
authors.affiliation: Department of Research and Postgraduate in Foods, University of Sonora, Blvd. Luis Encinas y Rosales s/n, Col. Centro, Apdo. Postal 1658, 83000, Hermosillo, Sonora, Mexico.
authors.forename: Enrique
authors.initials: E
authors.lastname: Márquez-Ríos
authors.affiliation: Department of Research and Postgraduate in Foods, University of Sonora, Blvd. Luis Encinas y Rosales s/n, Col. Centro, Apdo. Postal 1658, 83000, Hermosillo, Sonora, Mexico.
authors.forename: Wilfrido
authors.initials: W
authors.lastname: Torres-Arreola
authors.affiliation: Department of Chemical and Biological Sciences, University of Sonora, Blvd. Luis Encinas y Rosales s/n, Col. Centro, 83000, Hermosillo, Sonora, Mexico.
authors.forename: Aldo A
authors.initials: AA
authors.lastname: Arvizu-Flores
authors.affiliation: Department of Research and Postgraduate in Foods, University of Sonora, Blvd. Luis Encinas y Rosales s/n, Col. Centro, Apdo. Postal 1658, 83000, Hermosillo, Sonora, Mexico. ezquerra@guayacan.uson.mx.
authors.forename: J Marina
authors.initials: JM
authors.lastname: Ezquerra-Brauer
mesh_terms: D016604:Aflatoxin B1 / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors*; D000818:Animals; D004791:Enzyme Inhibitors / Q000378:metabolism*; D037341:Fumonisins / Q000378:metabolism*; D043143:Hepatopancreas / Q000201:enzymology*; D020128:Inhibitory Concentration 50; D033561:Penaeidae / Q000201:enzymology*
chemical_list: D004791:Enzyme Inhibitors; D037341:Fumonisins; C056933:fumonisin B1; D016604:Aflatoxin B1; D000469:Alkaline Phosphatase
keywords: Aflatoxin B1; Alkaline phosphatase; Fumonisin B1; Hepatopancreas; Litopenaeus vannamei; Mycotoxins