title: Bioremediation of polychlorinated-p-dioxins/dibenzofurans contaminated soil using simulated compost-amended landfill reactors under hypoxic conditions.
abstract: Compost-amended landfill reactors were developed to reduce polychlorinated-p-dioxins and dibenzofurans (PCDD/Fs) in contaminated soils. By periodically recirculating leachate and suppling oxygen, the online monitoring of the oxidation reduction potential confirmed that the reactors were maintained under hypoxic conditions, with redox levels constantly fluctuating between -400 and +80mV. The subsequent reactor operation demonstrated that PCDD/F degradation in soil could be facilitated by amending compost originating from the cow manure and waste sludge and that the degradation might be affected by the availability of easily degradable substrates in the soil and compost. The pyrosequencing analysis of V4/V5 regions of bacterial 16S rRNA genes suggested that species richness of the soil microbial community was increased by a factor of 1.37-1.61. Although the bacterial community varied with the compost origin and changed markedly during reactor operation, it was dominated by Alphaproteobacteria, Gammaproteobacteria, Actinobacteria, and Firmicutes. The aerotolerant anaerobic Sedimentibacter and Propionibacterium spp., and the uncultured Chloroflexi group could be temporarily induced to a high abundance by amending the cow manure compost; the bacterial growths were associated with the rapid degradation of PCDD/Fs. Overall, the novel bioremediation method for PCDD/F-contaminated soils using hypoxic conditions was effective, simple, energy saving, and thus easily practicable.
authors.affiliation: Department of Environmental Engineering, National Cheng Kung University, No.1, University Road, East District, Tainan City 701, Taiwan, ROC.
authors.forename: Wei-Yu
authors.initials: WY
authors.lastname: Chen
authors.affiliation: Department of Environmental Engineering, National Cheng Kung University, No.1, University Road, East District, Tainan City 701, Taiwan, ROC. Electronic address: enewujh@mail.ncku.edu.tw.
authors.forename: Jer-Horng
authors.initials: JH
authors.lastname: Wu
authors.affiliation: Department of Environmental Engineering, National Cheng Kung University, No.1, University Road, East District, Tainan City 701, Taiwan, ROC.
authors.forename: Shih-Chiang
authors.initials: SC
authors.lastname: Lin
authors.affiliation: Department of Environmental Engineering, National Cheng Kung University, No.1, University Road, East District, Tainan City 701, Taiwan, ROC.
authors.forename: Juu-En
authors.initials: JE
authors.lastname: Chang
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000378:metabolism; D001673:Biodegradation, Environmental; D002417:Cattle; D000072338:Dibenzofurans, Polychlorinated / Q000737:chemistry*; D004147:Dioxins / Q000737:chemistry*; D005260:Female; D008372:Manure; D012336:RNA, Ribosomal, 16S / Q000302:isolation & purification; D012722:Sewage; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000737:chemistry*; D064091:Waste Disposal Facilities
chemical_list: D000072338:Dibenzofurans, Polychlorinated; D004147:Dioxins; D008372:Manure; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D012989:Soil Pollutants; C038902:1,4-dioxin
keywords: Bioremediation; Compost; Hypoxic condition; Polychlorinated dioxins