title: A (1)H HR-MAS NMR-Based Metabolomic Study for Metabolic Characterization of Rice Grain from Various Oryza sativa L. Cultivars.
abstract: Rice grain metabolites are important for better understanding of the plant physiology of various rice cultivars and thus for developing rice cultivars aimed at providing diverse processed products. However, the variation of global metabolites in rice grains has rarely been explored. Here, we report the identification of intra- or intercellular metabolites in rice (Oryza sativa L.) grain powder using a (1)H high-resolution magic angle spinning (HR-MAS) NMR-based metabolomic approach. Compared with nonwaxy rice cultivars, marked accumulation of lipid metabolites such as fatty acids, phospholipids, and glycerophosphocholine in the grains of waxy rice cultivars demonstrated the distinct metabolic regulation and adaptation of each cultivar for effective growth during future germination, which may be reflected by high levels of glutamate, aspartate, asparagine, alanine, and sucrose. Therefore, this study provides important insights into the metabolic variations of diverse rice cultivars and their associations with environmental conditions and genetic backgrounds, with the aim of facilitating efficient development and the improvement of rice grain quality through inbreeding with genetic or chemical modification and mutation.
authors.affiliation: Division of Food and Nutrition, Chonnam National University , Yongbong-ro, Buk-gu, Gwangju 500-757, Republic of Korea.
authors.forename: Eun-Hye
authors.initials: EH
authors.lastname: Song
authors.affiliation: Western Seoul Center, Korea Basic Science Institute , Seoul 136-701, Republic of Korea.
authors.forename: Hyun-Ju
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Statistics, Chonnam National University , Yongbong-ro, Buk-gu, Gwangju 500-757, Republic of Korea.
authors.forename: Jaesik
authors.initials: J
authors.lastname: Jeong
authors.affiliation: Division of Food and Nutrition, Chonnam National University , Yongbong-ro, Buk-gu, Gwangju 500-757, Republic of Korea.
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Chung
authors.affiliation: Department of Applied Plant Science, Chonnam National University , Yongbong-ro, Buk-gu, Gwangju 500-757, Republic of Korea.
authors.forename: Han-Yong
authors.initials: HY
authors.lastname: Kim
authors.affiliation: Western Seoul Center, Korea Basic Science Institute , Seoul 136-701, Republic of Korea.
authors.forename: Eunjung
authors.initials: E
authors.lastname: Bang
authors.affiliation: Division of Food and Nutrition, Chonnam National University , Yongbong-ro, Buk-gu, Gwangju 500-757, Republic of Korea.
authors.forename: Young-Shick
authors.initials: YS
authors.lastname: Hong
mesh_terms: D002523:Edible Grain / Q000737:chemistry* / Q000235:genetics; D014644:Genetic Variation; D055432:Metabolomics; D019906:Nuclear Magnetic Resonance, Biomolecular; D012275:Oryza / Q000737:chemistry* / Q000235:genetics
keywords: cultivars; grain; high-resolution magic angle spinning (HR-MAS) NMR; metabolomics; rice