title: Protective Effects of Danlou Tablet () against Murine Myocardial Ischemia and Reperfusion Injury In Vivo.
abstract: OBJECTIVE
  To observe the in vivo effect of Danlou Tablet (, DLT) on myocardial ischemia and reperfusion (I/R) injury.
  
  
  METHODS
  DLT effects were evaluated in mouse heart preparation using 30-min coronary occlusion followed by 24-h reperfusion and compared among sham group (n=6), I/R group (n=8), IPC group (ischemia preconditioning, n=6) and DLT group (I/R with DLT pretreatment for 3 days, 750 mg•kg-1•day-1, n=8). The effects of DLT were characterized in infarction size (IS) compared with risk region (RR) and left ventricle using the Evans blue/triphenyltetrazolium chloride double dye staining method in vivo. Furthermore, the dose-dependent effect of DLT on I/R injury was evaluated by double staining method. Five different concentrations of DLT (0.625, 1.25, 2.5, 5 and 10 g•kg-1•day-1) were chosen in this study, and dose-response curve of DLT was obtained on these data.
  
  
  RESULTS
  The ratio of IS to left ventricle was significantly smaller in the DLT and IPC groups than the I/R group (P<0.05 or P<0.01), the ratio of IS to RR was also reduced in the DLT and IPC groups (P<0.01), while there were no differences in RR among the four groups (P>0.05). Experiments showed incidence of arrhythmias was reduced in the DLT group (P<0.01). Furthermore, DLT produced a dose-dependent inhibitory effect with a half maximal inhibitory concentration of 1.225 g•kg-1•day-1.
  
  
  CONCLUSIONS
  Our research concluded that DLT was effective in reducing I/R injury in mice, and provided experimental supports for the clinical use of DLT.
authors.affiliation: Intensive Care Laboratory, Guangdong Province Hospital of Chinese Medicine, The 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, China.
authors.forename: Jian-Yong
authors.initials: JY
authors.lastname: Qi
authors.affiliation: Intensive Care Laboratory, Guangdong Province Hospital of Chinese Medicine, The 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Rheumatology, The 3rd Affiliated Hospital of Southern Medical University, Guangzhou, 510630, China.
authors.forename: Dong-Sheng
authors.initials: DS
authors.lastname: Gu
authors.affiliation: Intensive Care Laboratory, Guangdong Province Hospital of Chinese Medicine, The 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, China.
authors.forename: Li-Heng
authors.initials: LH
authors.lastname: Guo
authors.affiliation: Network Pharmacology, Guangdong Province Hospital of Chinese Medicine, The 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510006, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Intensive Care Laboratory, Guangdong Province Hospital of Chinese Medicine, The 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, China. minzhouzhang8@163.com.
authors.forename: Min-Zhou
authors.initials: MZ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001145:Arrhythmias, Cardiac / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology; D001831:Body Temperature / Q000187:drug effects; D002316:Cardiotonic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D006339:Heart Rate / Q000187:drug effects; D006352:Heart Ventricles / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D008297:Male; D008810:Mice, Inbred C57BL; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D012307:Risk Factors; D013607:Tablets
chemical_list: D002316:Cardiotonic Agents; D004365:Drugs, Chinese Herbal; D013607:Tablets
keywords: Chinese medicine; Danlou Tablet; ischemia and reperfusion