title: Correlation of Wnt and NOTCH pathways in esophageal squamous cell carcinoma.
abstract: There is an inevitable association between cell signaling pathways and tumorigenesis. Wnt and notch pathways play important roles during development and self-renewal. Beside the independent role of such pathways on tumor progression, different cross talks between these pathways through tumorigenesis are emphasized. In this study, we analyzed cross talk between Wnt and NOTCH signaling pathways through assessment of probable correlation between MAML1 and PYGO2 as the main transcription factors of these pathways, respectively in esophageal squamous cell carcinoma (ESCC) patients. Levels of MAML1 and PYGO2 mRNA expression in 48 ESCC patients were compared to the correlated margin normal tissues using real-time polymerase chain reaction (PCR). Eleven out of 48 patients (22.9 %) have shown the concomitant MAML1/PYGO2 over expression in significant correlation with tumor size (p = 0.046) and depth of tumor invasion (p = 0.050). We showed that there is a significant correlation and feedback between these markers during the ESCC progression and metastasis.
authors.affiliation: Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Meysam
authors.initials: M
authors.lastname: Moghbeli
authors.affiliation: Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran.|Medical Genetics Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Abbaszadegan
authors.affiliation: Department of Anesthesiology and Critical Care, Mashhad University of Medical Sciences, Mashhad, Iran.|Addiction and Behavioral Sciences Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Golmakani
authors.affiliation: Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, 9613596135, Sabzevar, Iran. forghanifard@gmail.com.
authors.forename: Mohammad Mahdi
authors.initials: MM
authors.lastname: Forghanifard
keywords: Cancer stem cell; Crosstalk; Feedback; Gene expression; Real time PCR; Signaling pathway