title: Inhibition of Liver Carcinoma Cell Invasion and Metastasis by Knockdown of Cullin7 In Vitro and In Vivo.
abstract: Cullin7 is an E3 ubiquitin ligase. The Cullin7 protein family functions as a molecular scaffold to coordinate substrate ubiquitination in Skp, Cullin, and F-box-containing complex (SCF complex). Cullin7s control normal development and primary cellular processes and are characterized by a unique genomic network organization. Less is known about the involvement of Cullin7 with hepatocellular carcinoma (HCC). In this study, we found that Cullin7 showed a high expression in HCC tumor tissues, especially in metastatic HCC tumor tissues. Also, there was a negative correlation between Cullin7 expression and long survival. Silencing of Cullin7 in liver cancer cells can significantly reduce the migration, invasion, and metastatic abilities. Also, detection of epithelial-mesenchymal transition (EMT) marker expression showed that Cullin7 promotes epithelial-mesenchymal transformation of cancer cells. The results of this study helped to elucidate the oncogene functions of Cullin7 in liver cancers.
authors.affiliation: Second Ward of Infectious Disease Department, Linyi People's Hospital, Linyi, Shandong, China.
authors.forename: Donghui
authors.initials: D
authors.lastname: Zhang
authors.forename: Genling
authors.initials: G
authors.lastname: Yang
authors.forename: Xidong
authors.initials: X
authors.lastname: Li
authors.forename: Cheng
authors.initials: C
authors.lastname: Xu
authors.forename: Honglei
authors.initials: H
authors.lastname: Ge
mesh_terms: D000818:Animals; D044842:Cullin Proteins / Q000235:genetics* / Q000378:metabolism; D058750:Epithelial-Mesenchymal Transition; D055785:Gene Knockdown Techniques; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology* / Q000473:pathology; D008175:Lung Neoplasms / Q000201:enzymology* / Q000556:secondary; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation
chemical_list: C506299:CUL7 protein, human; D044842:Cullin Proteins