title: Regulation of Arabidopsis thaliana plasma membrane glucose-responsive regulator (AtPGR) expression by A. thaliana storekeeper-like transcription factor, AtSTKL, modulates glucose response in Arabidopsis.
abstract: Biochemical, genetic, physiological, and molecular research in plants has demonstrated a central role of glucose (Glc) in the control of plant growth, metabolism, and development, and has revealed networks that integrate light, stresses, nutrients, and hormone signaling. Previous studies have reported that AtPGR protein as potential candidates for Glc signaling protein. In the present study, we characterized transcription factors that bind to the upstream region of the AtPGR gene isolated using the yeast one-hybrid screening with an Arabidopsis cDNA library. One of the selected genes (AtSTKL) appeared to confer elevated sensitivity to Glc response. Overexpression of AtSTKLs (AtSTKL1 and AtSTKL2) increased the sensitivity to Glc during the post-germination stages. In contrast, atstkl1 and atstkl2 antisense lines displayed reduced sensitivity to high Glc concentration during the early seedling stage. Furthermore, we showed that the two AtSTKLs bind to the 5'-GCCT-3' element of the upstream promoter region of the AtPGR gene in vitro and repress the beta-glucuronidase (GUS) activity in AtPGR promoter-GUS (P999-GUS) transgenic plants. Green fluorescent protein (GFP)-tagged AtSTKLs were localized in the nuclei of transgenic Arabidopsis cells. Collectively, these results suggest that AtSTKL1 and AtSTKL2 function both as repressors of AtPGR transcription and as novel transcription factors in the Glc signaling pathway.
authors.affiliation: Korea Atomic Energy Research Institute, Jeollabuk-do 580-185, South Korea; Department of Plant Biotechnology, Chonnam National University, Gwangju 500-757, South Korea.
authors.forename: Moon-Soo
authors.initials: MS
authors.lastname: Chung
authors.affiliation: Korea Atomic Energy Research Institute, Jeollabuk-do 580-185, South Korea.
authors.forename: Sungbeom
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Plant Biotechnology, Chonnam National University, Gwangju 500-757, South Korea.
authors.forename: Ji-Hee
authors.initials: JH
authors.lastname: Min
authors.affiliation: Department of Plant Biotechnology, Chonnam National University, Gwangju 500-757, South Korea.
authors.forename: Ping
authors.initials: P
authors.lastname: Huang
authors.affiliation: Department of Plant Biotechnology, Chonnam National University, Gwangju 500-757, South Korea.
authors.forename: Hyun-Woo
authors.initials: HW
authors.lastname: Ju
authors.affiliation: Department of Plant Biotechnology, Chonnam National University, Gwangju 500-757, South Korea. Electronic address: cskim626@chonnam.ac.kr.
authors.forename: Cheol Soo
authors.initials: CS
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D018548:Cotyledon / Q000187:drug effects / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D024202:Electrophoretic Mobility Shift Assay; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D005947:Glucose / Q000494:pharmacology*; D005966:Glucuronidase / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011485:Protein Binding / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D017384:Sequence Deletion; D014158:Transcription, Genetic / Q000187:drug effects; D015533:Transcriptional Activation / Q000187:drug effects / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D018744:DNA, Plant; D008565:Membrane Proteins; C000616055:PGR protein, Arabidopsis; D012333:RNA, Messenger; D012097:Repressor Proteins; C000616052:STKL1 protein, Arabidopsis; C000616053:STKL2 protein, Arabidopsis; D005966:Glucuronidase; D005947:Glucose
keywords: AtPGR; AtSTKL; Glucose-responsive element; Repressor; Transcription factor