title: Identification of galectin-1 as a novel mediator for chemoresistance in chronic myeloid leukemia cells.
abstract: Multidrug resistance protein-1 (MDR1) has been proven to be associated with the development of chemoresistance to imatinib (Glivec, STI571) which displays high efficacy in treatment of BCR-ABL-positive chronic myelogenous leukemia (CML). However, the possible mechanisms of MDR1 modulation in the process of the resistance development remain to be defined. Herein, galectin-1 was identified as a candidate modulator of MDR1 by proteomic analysis of a model system of leukemia cell lines with a gradual increase of MDR1 expression and drug resistance. Coincidently, alteration of galectin-1 expression triggers the change of MDR1 expression as well as the resistance to the cytotoxic drugs, suggesting that augment of MDR1 expression engages in galectin-1-mediated chemoresistance. Moreover, we provided the first data showing that NF-κB translocation induced by P38 MAPK activation was responsible for the modulation effect of galectin-1 on MDR1 in the chronic myelogenous leukemia cells. Galectin-1 might be considered as a novel target for combined modality therapy for enhancing the efficacy of CML treatment with imatinib.
authors.affiliation: Institute of Tissue Transplantation and Immunology, Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Key Laboratory of Molecule Immunology and Antibody Engineering of Guangdong Province, Jinan University, Guangzhou, 510632, China.
authors.forename: Wu
authors.initials: W
authors.lastname: Luo
authors.affiliation: Institute of Tissue Transplantation and Immunology, Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Key Laboratory of Molecule Immunology and Antibody Engineering of Guangdong Province, Jinan University, Guangzhou, 510632, China.
authors.forename: Li
authors.initials: L
authors.lastname: Song
authors.affiliation: Institute of Tissue Transplantation and Immunology, Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Key Laboratory of Molecule Immunology and Antibody Engineering of Guangdong Province, Jinan University, Guangzhou, 510632, China.
authors.forename: Xi-Lei
authors.initials: XL
authors.lastname: Chen
authors.affiliation: Institute of Tissue Transplantation and Immunology, Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Key Laboratory of Molecule Immunology and Antibody Engineering of Guangdong Province, Jinan University, Guangzhou, 510632, China.
authors.forename: Xiang-Feng
authors.initials: XF
authors.lastname: Zeng
authors.affiliation: School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Jian-Zhang
authors.initials: JZ
authors.lastname: Wu
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou, 510623, China.
authors.forename: Cai-Rong
authors.initials: CR
authors.lastname: Zhu
authors.affiliation: Institute of Tissue Transplantation and Immunology, Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Key Laboratory of Molecule Immunology and Antibody Engineering of Guangdong Province, Jinan University, Guangzhou, 510632, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Huang
authors.affiliation: Institute of Tissue Transplantation and Immunology, Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Key Laboratory of Molecule Immunology and Antibody Engineering of Guangdong Province, Jinan University, Guangzhou, 510632, China.
authors.forename: Xiang-Peng
authors.initials: XP
authors.lastname: Tan
authors.affiliation: Institute of Tissue Transplantation and Immunology, Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Key Laboratory of Molecule Immunology and Antibody Engineering of Guangdong Province, Jinan University, Guangzhou, 510632, China.
authors.forename: Xiao-Mian
authors.initials: XM
authors.lastname: Lin
authors.affiliation: Institute of Tissue Transplantation and Immunology, Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Key Laboratory of Molecule Immunology and Antibody Engineering of Guangdong Province, Jinan University, Guangzhou, 510632, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Institute of Tissue Transplantation and Immunology, Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Key Laboratory of Molecule Immunology and Antibody Engineering of Guangdong Province, Jinan University, Guangzhou, 510632, China.
authors.forename: Ji-Zhong
authors.initials: JZ
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Xiao-Kun
authors.initials: XK
authors.lastname: Li
authors.affiliation: Institute of Tissue Transplantation and Immunology, Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Key Laboratory of Molecule Immunology and Antibody Engineering of Guangdong Province, Jinan University, Guangzhou, 510632, China.|School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Wu
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000378:metabolism; D019008:Drug Resistance, Neoplasm / Q000502:physiology*; D037483:Galectin 1 / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000378:metabolism / Q000473:pathology*; D040901:Proteomics
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D037483:Galectin 1
keywords: MDR1; P38 MAPK; chemoresistance; chronic myelogenous leukemia; galectin-1