title: Microfluidic techniques for high throughput single cell analysis.
abstract: The microfabrication of microfluidic control systems and the development of increasingly sensitive molecular amplification tools have enabled the miniaturization of single cells analytical platforms. Only recently has the throughput of these platforms increased to a level at which populations can be screened at the single cell level. Techniques based upon both active and passive manipulation are now capable of discriminating between single cell phenotypes for sorting, diagnostic or prognostic applications in a variety of clinical scenarios. The introduction of multiphase microfluidics enables the segmentation of single cells into biochemically discrete picoliter environments. The combination of these techniques are enabling a class of single cell analytical platforms within great potential for data driven biomedicine, genomics and transcriptomics.
authors.affiliation: Department of Chemical Engineering, University of Wyoming, 1000 East University Avenue, Laramie, WY 82070, United States.
authors.forename: Amy
authors.initials: A
authors.lastname: Reece
authors.affiliation: Department of Chemical Engineering, University of Wyoming, 1000 East University Avenue, Laramie, WY 82070, United States.
authors.forename: Bingzhao
authors.initials: B
authors.lastname: Xia
authors.affiliation: Department of Chemical Engineering, University of Wyoming, 1000 East University Avenue, Laramie, WY 82070, United States.
authors.forename: Zhongliang
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Department of Chemical Engineering, University of Wyoming, 1000 East University Avenue, Laramie, WY 82070, United States.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Noren
authors.affiliation: Department of Chemical Engineering, University of Wyoming, 1000 East University Avenue, Laramie, WY 82070, United States.
authors.forename: Ralph
authors.initials: R
authors.lastname: McBride
authors.affiliation: Department of Chemical Engineering, University of Wyoming, 1000 East University Avenue, Laramie, WY 82070, United States. Electronic address: joakey@uwyo.edu.
authors.forename: John
authors.initials: J
authors.lastname: Oakey
mesh_terms: D000818:Animals; D057166:High-Throughput Screening Assays; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D059010:Single-Cell Analysis / Q000379:methods*