title: Expression Profile of Long Non-Coding RNAs in Serum of Patients with Multiple Sclerosis.
abstract: Multiple sclerosis (MS) is a chronic progressive inflammatory disease of the central nervous system (CNS) that leads to severe neurological disability. There is an interest in potential biomarkers that could provide information predicting disease activity and progression. Long non-coding RNAs (lncRNAs) have been reported to be involved in the pathogenesis of various human disorders, such as oncologic, cardiovascular, and neurodegenerative diseases. No studies have so far explored a potential link between lncRNAs and MS pathology. We screened 84 lncRNAs, involved in autoimmunity and human inflammatory response, in the serum of relapsing-remitting MS (RR-MS) patients (n = 12), age-matched controls (n = 12), and in patients with idiopathic inflammatory myopathy (IIM) (n = 12). We used the following criteria for lncRNAs analysis: fold change >2 and p < 0.05. According to these criteria, by real-time PCR, we identified three lncRNAs up-regulated in RR-MS patients respectively to controls: nuclear paraspeckle assembly transcript 1 (NEAT1), taurine up-regulated 1 (TUG1), and 7SK small nuclear (RN7SK RNA). Literature data showed that NEAT1, TUG1, and RN7SK RNA play an important role in neurodegenerative processes. Our results indicate that these lncRNAs may be involved in MS pathogenesis. Additional experimental data are needed to clarify the molecular mechanisms through which lncRNAs up-regulation may have a role in MS.
authors.affiliation: Fondazione Don Carlo Gnocchi, Via Capecelatro 66, 20148, Milan, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Santoro
authors.affiliation: Fondazione Don Carlo Gnocchi, Via Capecelatro 66, 20148, Milan, Italy. viviana.nociti@policlinicogemelli.it.|Department of Geriatrics, Neuroscience and Orthopedics, Institute of Neurology, UCSC, Largo F. Vito 1, 00168, Rome, Italy. viviana.nociti@policlinicogemelli.it.
authors.forename: Viviana
authors.initials: V
authors.lastname: Nociti
authors.affiliation: Department of Geriatrics, Neuroscience and Orthopedics, Institute of Neurology, UCSC, Largo F. Vito 1, 00168, Rome, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Lucchini
authors.affiliation: Department of Geriatrics, Neuroscience and Orthopedics, Institute of Neurology, UCSC, Largo F. Vito 1, 00168, Rome, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: De Fino
authors.affiliation: Department of Geriatrics, Neuroscience and Orthopedics, Institute of Neurology, UCSC, Largo F. Vito 1, 00168, Rome, Italy.
authors.forename: Francesco Antonio
authors.initials: FA
authors.lastname: Losavio
authors.affiliation: Department of Geriatrics, Neuroscience and Orthopedics, Institute of Neurology, UCSC, Largo F. Vito 1, 00168, Rome, Italy.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Mirabella
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood*; D062085:RNA, Long Noncoding / Q000097:blood*
chemical_list: D015415:Biomarkers; C577276:NEAT1 long non-coding RNA, human; D062085:RNA, Long Noncoding; C000598536:TUG1 long noncoding RNA, human; C000610089:long non-coding RNA 7SK, human
keywords: Autoimmunity; Long non-coding RNA; Multiple sclerosis; Neurodegeneration