title: Physiological Concentration of Exogenous Lactate Reduces Antimycin A Triggered Oxidative Stress in Intestinal Epithelial Cell Line IPEC-1 and IPEC-J2 In Vitro.
abstract: Weaning triggers an adaptation of the gut function including luminal lactate generation by lactobacilli, depending on gastrointestinal site. We hypothesized that both lactobacilli and lactate influence porcine intestinal epithelial cells. In vivo experiments showed that concentration of lactate was significantly higher in gastric, duodenal and jejunal chyme of suckling piglets compared to their weaned counterparts. In an in vitro study we investigated the impact of physiological lactate concentration as derived from the in vivo study on the porcine intestinal epithelial cells IPEC-1 and IPEC-J2. We detected direct adherence of lactobacilli on the apical epithelial surface and a modulated F-actin structure. Application of lactobacilli culture supernatant alone or lactate (25 mM) at low pH (pH 4) changed the F-actin structure in a similar manner. Treatment of IPEC cultures with lactate at near neutral pH resulted in a significantly reduced superoxide-generation in Antimycin A-challenged cells. This protective effect was nearly completely reversed by inhibition of cellular lactate uptake via monocarboxylate transporter. Lactate treatment enhanced NADH autofluorescence ratio (Fcytosol/Fnucleus) in non-challenged cells, indicating an increased availability of reduced nucleotides, but did not change the overall ATP content of the cells. Lactobacilli-derived physiological lactate concentration in intestine is relevant for alleviation of redox stress in intestinal epithelial cells.
authors.affiliation: Institute of Anatomy, Otto von Guericke University Magdeburg, Magdeburg, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Kahlert
authors.affiliation: Department of Veterinary Biosciences, University of Helsinki, Helsinki, Finland.
authors.forename: Sami
authors.initials: S
authors.lastname: Junnikkala
authors.affiliation: Institute of Anatomy, Otto von Guericke University Magdeburg, Magdeburg, Germany.
authors.forename: Lydia
authors.initials: L
authors.lastname: Renner
authors.affiliation: Department of Veterinary Biosciences, University of Helsinki, Helsinki, Finland.
authors.forename: Ulla
authors.initials: U
authors.lastname: Hynönen
authors.affiliation: Institute of Molecular and Clinical Immunology, Otto von Guericke University Magdeburg, Magdeburg, Germany.
authors.forename: Roland
authors.initials: R
authors.lastname: Hartig
authors.affiliation: Institute of Anatomy, Otto von Guericke University Magdeburg, Magdeburg, Germany.
authors.forename: Constanze
authors.initials: C
authors.lastname: Nossol
authors.affiliation: Institute of Anatomy, Otto von Guericke University Magdeburg, Magdeburg, Germany.
authors.forename: Anikó
authors.initials: A
authors.lastname: Barta-Böszörményi
authors.affiliation: Institute of Animal Nutrition, Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, Braunschweig, Germany.
authors.forename: Sven
authors.initials: S
authors.lastname: Dänicke
authors.affiliation: Research Unit for Nutritional Physiology 'Oskar Kellner', Leibniz Institute for Farm Animal Biology, Dummerstorf, Germany.
authors.forename: Wolfgang-Bernhard
authors.initials: WB
authors.lastname: Souffrant
authors.affiliation: Department of Veterinary Biosciences, University of Helsinki, Helsinki, Finland.
authors.forename: Airi
authors.initials: A
authors.lastname: Palva
authors.affiliation: Institute of Anatomy, Otto von Guericke University Magdeburg, Magdeburg, Germany.
authors.forename: Hermann-Josef
authors.initials: HJ
authors.lastname: Rothkötter
authors.affiliation: Institute of Animal Nutrition, Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, Braunschweig, Germany.
authors.forename: Jeannette
authors.initials: J
authors.lastname: Kluess
mesh_terms: D000199:Actins / Q000737:chemistry / Q000187:drug effects; D000818:Animals; D000968:Antimycin A / Q000494:pharmacology*; D001422:Bacterial Adhesion; D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000382:microbiology; D005260:Female; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology* / Q000382:microbiology; D019344:Lactic Acid / Q000494:pharmacology*; D007778:Lactobacillus / Q000502:physiology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D013552:Swine
chemical_list: D000199:Actins; D019344:Lactic Acid; D000968:Antimycin A