title: Nutritional Omega-3 Deficiency Alters Glucocorticoid Receptor-Signaling Pathway and Neuronal Morphology in Regionally Distinct Brain Structures Associated with Emotional Deficits.
abstract: Extensive evidence suggests that long term dietary n-3 polyunsaturated fatty acids (PUFAs) deficiency results in altered emotional behaviour. We have recently demonstrated that n-3 PUFAs deficiency induces emotional alterations through abnormal corticosterone secretion which leads to altered dendritic arborisation in the prefrontal cortex (PFC). Here we show that hypothalamic-pituitary-adrenal (HPA) axis feedback inhibition was not compromised in n-3 deficient mice. Rather, glucocorticoid receptor (GR) signaling pathway was inactivated in the PFC but not in the hippocampus of n-3 deficient mice. Consequently, only dendritic arborisation in PFC was affected by dietary n-3 PUFAs deficiency. In addition, occlusion experiment with GR blockade altered GR signaling in the PFC of control mice, with no further alterations in n-3 deficient mice. In conclusion, n-3 PUFAs deficiency compromised PFC, leading to dendritic atrophy, but did not change hippocampal GR function and dendritic arborisation. We argue that this GR sensitivity contributes to n-3 PUFAs deficiency-related emotional behaviour deficits.
authors.affiliation: INRA, Nutrition et Neurobiologie Intégrée, UMR 1286, 33076 Bordeaux, France; Univ. Bordeaux, Nutrition et Neurobiologie Intégrée, UMR 1286, 33076 Bordeaux, France; Brain Mind Institute, School of Life Sciences, École Polytechnique Federale de Lausanne (EPFL), 1050 Lausanne, Switzerland.
authors.forename: Thomas
authors.initials: T
authors.lastname: Larrieu
authors.affiliation: INSERM, Neurocentre Magendie, UMR 862, 33077 Bordeaux, France; Univ. Bordeaux, Neurocentre Magendie, UMR 862, 33077 Bordeaux, France.
authors.forename: Muna L
authors.initials: ML
authors.lastname: Hilal
authors.affiliation: INRA, Nutrition et Neurobiologie Intégrée, UMR 1286, 33076 Bordeaux, France; Univ. Bordeaux, Nutrition et Neurobiologie Intégrée, UMR 1286, 33076 Bordeaux, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: De Smedt-Peyrusse
authors.affiliation: INSERM, Neurocentre Magendie, UMR 862, 33077 Bordeaux, France; Univ. Bordeaux, Neurocentre Magendie, UMR 862, 33077 Bordeaux, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Sans
authors.affiliation: INRA, Nutrition et Neurobiologie Intégrée, UMR 1286, 33076 Bordeaux, France; Univ. Bordeaux, Nutrition et Neurobiologie Intégrée, UMR 1286, 33076 Bordeaux, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Layé
mesh_terms: D000818:Animals; D048430:Cell Shape / Q000502:physiology; D004644:Emotions / Q000502:physiology*; D015525:Fatty Acids, Omega-3; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D007030:Hypothalamo-Hypophyseal System / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D010913:Pituitary-Adrenal System / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D015525:Fatty Acids, Omega-3; D011965:Receptors, Glucocorticoid