title: Application of spectroscopy (1HMRS) to assess liver metabolite concentrations in rats with intrauterine growth restriction.
abstract: OBJECTIVE
  Proton magnetic resonance spectroscopy (1H-MRS) measurement of liver metabolism in intrauterine growth restriction rats has seldom been reported. This study investigated the application of1H-MRS in assessing liver metabolism in newborn pups that experienced intrauterine growth restriction.
  
  
  METHODS
  Intra-uterine growth restriction was established by feeding rats low-protein diets during pregnancy. Newborn pups received conventional magnetic resonance imaging and1H-MRS using a 3.0T whole body MR scanner at 3, 8 and 12 weeks post birth.
  
  
  RESULTS
  The success rate of1H-MRS was 83.33%. Significantly lower body weight, BMI and body length at 3 weeks as well as significantly lower body weight, BMI and waist circumference at 8 and 12 weeks were observed in newborn pups of IUGR rats compared with pups of control rats. Significant differences in ACho/H2O, ACr/H2O, AGlx/H2O and ALipid/H2O at 3 and 8 weeks as well as significant differences in ACr/H2O, ALipid/H2O and AGlx/H2O at 12 weeks were observed between pups of control rats and pups of IUGR rats.
  
  
  CONCLUSIONS
  1H-MRS allows noninvasive assessment of liver metabolism in the rat and demonstrated the poor liver development of rats that experienced IUGR.
authors.affiliation: a Department of Neonatology , Children's Medical Center, Second Xiangya Hospital, Central South University , Changsha , P.R. China and.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: a Department of Neonatology , Children's Medical Center, Second Xiangya Hospital, Central South University , Changsha , P.R. China and.
authors.forename: Pingyang
authors.initials: P
authors.lastname: Chen
authors.affiliation: b Department of Radiology , Second Xiangya Hospital, Central South University , Changsha , P.R. China.
authors.forename: Dujun
authors.initials: D
authors.lastname: Bian
authors.affiliation: a Department of Neonatology , Children's Medical Center, Second Xiangya Hospital, Central South University , Changsha , P.R. China and.
authors.forename: Juncao
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002794:Choline / Q000032:analysis / Q000378:metabolism; D003401:Creatine / Q000032:analysis / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005317:Fetal Growth Retardation / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D005947:Glucose / Q000032:analysis / Q000378:metabolism; D006003:Glycogen / Q000032:analysis / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000737:chemistry* / Q000000981:diagnostic imaging* / Q000378:metabolism; D011247:Pregnancy; D066244:Proton Magnetic Resonance Spectroscopy; D051381:Rats; D017207:Rats, Sprague-Dawley; D014867:Water / Q000032:analysis / Q000378:metabolism
chemical_list: D008055:Lipids; D014867:Water; D006003:Glycogen; D005947:Glucose; D003401:Creatine; D002794:Choline
keywords: Intrauterine growth retardation; liver metabolism; proton magnetic resonance spectroscopy