title: Evaluation of Tazobactam-Supplemented, Modified Charcoal-Cefoperazone-Deoxycholate Agar for Qualitative Detection of Campylobacter from Chicken Carcass Rinse.
abstract: Overgrowth of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli on modified charcoal-cefoperazone-deoxycholate agar (mCCDA) is the most common confounding factor for the isolation of Campylobacter from poultry samples. mCCDA modified by supplementation with tazobactam, an ESBL inhibitor, was evaluated for Campylobacter isolation from chicken carcass rinse with regard to isolation rate and selectivity. In total, 120 whole chicken carcasses purchased from retail stores were rinsed with buffered peptone water enriched with 2× blood-free Bolton broth at 42°C for 48 h and then inoculated onto mCCDA with and without tazobactam supplementation (mCCDA or T-mCCDA) at 42°C for 48 h under microaerobic conditions. Suspect colonies were subcultured and confirmed by colony PCR. Plates with tazobactam exhibited a higher Campylobacter isolation rate (56.7% vs. 30.8%, p < 0.05) and selectivity (0.8 vs. 83.3% plates contaminated with non-Campylobacter, p < 0.05) than mCCDA. Thus, tazobactam-supplemented mCCDA would be a useful option for qualitative detection of Campylobacter in chicken carcass rinse.
authors.affiliation: 1 Department of Animal Science and Technology, Konkuk University , Seoul, Republic of Korea.
authors.forename: Jung-Whan
authors.initials: JW
authors.lastname: Chon
authors.affiliation: 2 Center for One Health, College of Veterinary Medicine, Konkuk University , Seoul, Republic of Korea.
authors.forename: Young-Ji
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: 2 Center for One Health, College of Veterinary Medicine, Konkuk University , Seoul, Republic of Korea.
authors.forename: Hong-Seok
authors.initials: HS
authors.lastname: Kim
authors.affiliation: 2 Center for One Health, College of Veterinary Medicine, Konkuk University , Seoul, Republic of Korea.
authors.forename: Dong-Hyeon
authors.initials: DH
authors.lastname: Kim
authors.affiliation: 3 Department of Food and Nutrition, Kosin University , Pusan, Republic of Korea.
authors.forename: Dong Kwan
authors.initials: DK
authors.lastname: Jeong
authors.affiliation: 2 Center for One Health, College of Veterinary Medicine, Konkuk University , Seoul, Republic of Korea.
authors.forename: Kun-Ho
authors.initials: KH
authors.lastname: Seo
mesh_terms: D000362:Agar / Q000494:pharmacology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002167:Campylobacter / Q000187:drug effects / Q000302:isolation & purification* / Q000502:physiology; D002606:Charcoal / Q000494:pharmacology; D002645:Chickens / Q000382:microbiology; D003470:Culture Media; D003840:Deoxycholic Acid / Q000494:pharmacology; D005516:Food Microbiology; D010397:Penicillanic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D000078142:Tazobactam
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D003840:Deoxycholic Acid; D002606:Charcoal; D010397:Penicillanic Acid; D000362:Agar; D000078142:Tazobactam