title: The prostaglandin E2/EP4 receptor/cyclic AMP/T-type Ca(2+) channel pathway mediates neuritogenesis in sensory neuron-like ND7/23 cells.
abstract: We investigated mechanisms for the neuritogenesis caused by prostaglandin E2 (PGE2) or intracellular cyclic AMP (cAMP) in sensory neuron-like ND7/23 cells. PGE2 caused neuritogenesis, an effect abolished by an EP4 receptor antagonist or inhibitors of adenylyl cyclase (AC) or protein kinase A (PKA) and mimicked by the AC activator forskolin, dibutyryl cAMP (db-cAMP), and selective activators of PKA or Epac. ND7/23 cells expressed both Cav3.1 and Cav3.2 T-type Ca(2+) channels (T-channels). The neuritogenesis induced by db-cAMP or PGE2 was abolished by T-channel blockers. T-channels were functionally upregulated by db-cAMP. The PGE2/EP4/cAMP/T-channel pathway thus appears to mediate neuritogenesis in sensory neurons.
authors.affiliation: Laboratory of Pharmacology and Pathophysiology, Faculty of Pharmacy, Kindai University, (formerly Kinki University), Higashi-Osaka 577-8502, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Mitani
authors.affiliation: Laboratory of Pharmacology and Pathophysiology, Faculty of Pharmacy, Kindai University, (formerly Kinki University), Higashi-Osaka 577-8502, Japan.
authors.forename: Fumiko
authors.initials: F
authors.lastname: Sekiguchi
authors.affiliation: Laboratory of Pharmacology and Pathophysiology, Faculty of Pharmacy, Kindai University, (formerly Kinki University), Higashi-Osaka 577-8502, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Maeda
authors.affiliation: Laboratory of Pharmacology and Pathophysiology, Faculty of Pharmacy, Kindai University, (formerly Kinki University), Higashi-Osaka 577-8502, Japan.
authors.forename: Yukari
authors.initials: Y
authors.lastname: Tanaka
authors.affiliation: Department of Life Science, Faculty of Science and Engineering, Kindai University, Higashi-Osaka 577-8502, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Yoshida
authors.affiliation: Laboratory of Pharmacology and Pathophysiology, Faculty of Pharmacy, Kindai University, (formerly Kinki University), Higashi-Osaka 577-8502, Japan. Electronic address: kawabata@phar.kindai.ac.jp.
authors.forename: Atsufumi
authors.initials: A
authors.lastname: Kawabata
mesh_terms: D000818:Animals; D020747:Calcium Channels, T-Type / Q000494:pharmacology / Q000502:physiology*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000494:pharmacology / Q000502:physiology*; D015232:Dinoprostone / Q000494:pharmacology / Q000502:physiology*; D051379:Mice; D000071446:Neuronal Outgrowth / Q000187:drug effects* / Q000235:genetics*; D051381:Rats; D058309:Receptors, Prostaglandin E, EP4 Subtype / Q000502:physiology*; D011984:Sensory Receptor Cells / Q000166:cytology*; D015398:Signal Transduction
chemical_list: D020747:Calcium Channels, T-Type; D058309:Receptors, Prostaglandin E, EP4 Subtype; D000242:Cyclic AMP; D015232:Dinoprostone
keywords: Neuritogenesis; Sensory neuron; T-type calcium channel