title: Comparative Analysis of Cellular Immune Responses in Treated Leishmania Patients and Hamsters against Recombinant Th1 Stimulatory Proteins of Leishmania donovani.
abstract: Our prior studies demonstrated that cellular response of T helper 1 (Th1) type was generated by a soluble antigenic fraction (ranging from 89.9 to 97.1 kDa) of Leishmania donovani promastigote, in treated Leishmania patients as well as hamsters and showed significant prophylactic potential against experimental visceral leishmaniasis (VL). Eighteen Th1 stimulatory proteins were identified through proteomic analysis of this subfraction, out of which 15 were developed as recombinant proteins. In the present work, we have evaluated these 15 recombinant proteins simultaneously for their comparative cellular responses in treated Leishmania patients and hamsters. Six proteins viz. elongation factor-2, enolase, aldolase, triose phosphate isomerase, protein disulfide isomerase, and p45 emerged as most immunogenic as they produced a significant lymphoproliferative response, nitric oxide generation and Th1 cytokine response in PBMCs and lymphocytes of treated Leishmania patients and hamsters respectively. The results suggested that these proteins may be exploited for developing a successful poly-protein and/or poly-epitope vaccine against VL.
authors.affiliation: Parasitology Division, Council of Scientific and Industrial Research-Central Drug Research Institute Lucknow, India.
authors.forename: Sumit
authors.initials: S
authors.lastname: Joshi
authors.affiliation: Parasitology Division, Council of Scientific and Industrial Research-Central Drug Research Institute Lucknow, India.
authors.forename: Narendra K
authors.initials: NK
authors.lastname: Yadav
authors.affiliation: Parasitology Division, Council of Scientific and Industrial Research-Central Drug Research Institute Lucknow, India.
authors.forename: Keerti
authors.initials: K
authors.lastname: Rawat
authors.affiliation: Parasitology Division, Council of Scientific and Industrial Research-Central Drug Research Institute Lucknow, India.
authors.forename: Chandra Dev P
authors.initials: CD
authors.lastname: Tripathi
authors.affiliation: Parasitology Division, Council of Scientific and Industrial Research-Central Drug Research Institute Lucknow, India.
authors.forename: Anil K
authors.initials: AK
authors.lastname: Jaiswal
authors.affiliation: Parasitology Division, Council of Scientific and Industrial Research-Central Drug Research Institute Lucknow, India.
authors.forename: Prashant
authors.initials: P
authors.lastname: Khare
authors.affiliation: Parasitology Division, Council of Scientific and Industrial Research-Central Drug Research Institute Lucknow, India.
authors.forename: Rati
authors.initials: R
authors.lastname: Tandon
authors.affiliation: Parasitology Division, Council of Scientific and Industrial Research-Central Drug Research Institute Lucknow, India.
authors.forename: Rajendra K
authors.initials: RK
authors.lastname: Baharia
authors.affiliation: Parasitology Division, Council of Scientific and Industrial Research-Central Drug Research Institute Lucknow, India.
authors.forename: Sanchita
authors.initials: S
authors.lastname: Das
authors.affiliation: Parasitology Division, Council of Scientific and Industrial Research-Central Drug Research Institute Lucknow, India.
authors.forename: Reema
authors.initials: R
authors.lastname: Gupta
authors.affiliation: Parasitology Division, Council of Scientific and Industrial Research-Central Drug Research Institute Lucknow, India.
authors.forename: Pramod K
authors.initials: PK
authors.lastname: Kushawaha
authors.affiliation: Department of Medicine, Institute of Medical Sciences, Banaras Hindu University Varanasi, India.
authors.forename: Shyam
authors.initials: S
authors.lastname: Sundar
authors.affiliation: Molecular and Structural Biology Division, Council of Scientific and Industrial Research-Central Drug Research Institute Lucknow, India.
authors.forename: Amogh A
authors.initials: AA
authors.lastname: Sahasrabuddhe
authors.affiliation: Parasitology Division, Council of Scientific and Industrial Research-Central Drug Research Institute Lucknow, India.
authors.forename: Anuradha
authors.initials: A
authors.lastname: Dube
keywords: Leishmania donovani; PBMCs; cellular response; immunogenicity; lymphocytes; recombinant Th1 stimulatory proteins; treated Leishmania patients; treated Leishmania-infected hamster