title: Cell growth on 3D microstructured surfaces.
abstract: Chinese Hamster Ovary (CHO) cell cultures were grown on surfaces lithographed with periodic 3D hexagonal microcavity array morphology. The range of microcavity size (inscribed circle diameter) was from 12 μm to 560 μm. CHO cells were grown also on flat surfaces. The characterization was performed with respect to cell growth density (number of nuclei per unit area) by fluorescence optical microscopy and evaluated by correlation function analysis. We found that optimum microcavity radius was 80 μm, concerning to the maximum cell growth density, being even greater than the growth density on a flat (unstructured) substrate of the same material. This finding can be important for optimization of biotechnological processes and devices.
authors.affiliation: Institute of Physics, University of São Paulo, C.P. 66318, CEP 05315-970 São Paulo, SP, Brazil. Electronic address: wwlysses@if.usp.com.
authors.forename: W W R
authors.initials: WW
authors.lastname: Araujo
authors.affiliation: Institute of Physics, University of São Paulo, C.P. 66318, CEP 05315-970 São Paulo, SP, Brazil.
authors.forename: F S
authors.initials: FS
authors.lastname: Teixeira
authors.affiliation: Pharmacy School, UFOP - Federal University of Ouro Preto, Ouro Preto, MG, Brazil.
authors.forename: G N
authors.initials: GN
authors.lastname: da Silva
authors.affiliation: Botucatu Medical School, UNESP - São Paulo State University, Botucatu, SP, Brazil. Electronic address: mcsalva@if.usp.br.
authors.forename: D M F
authors.initials: DM
authors.lastname: Salvadori
authors.affiliation: Institute of Physics, University of São Paulo, C.P. 66318, CEP 05315-970 São Paulo, SP, Brazil.
authors.forename: M C
authors.initials: MC
authors.lastname: Salvadori
authors.affiliation: Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, United States.
authors.forename: I G
authors.initials: IG
authors.lastname: Brown
mesh_terms: D000818:Animals; D016466:CHO Cells; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D049109:Cell Proliferation / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D004852:Epoxy Compounds / Q000737:chemistry / Q000494:pharmacology; D007091:Image Processing, Computer-Assisted; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D013499:Surface Properties
chemical_list: D004852:Epoxy Compounds; D011108:Polymers; C520895:SU-8 compound
keywords: Biomaterials; Cell aggregation; Optical microscopy; Surface patterning