title: Role of Mas receptor in renal blood flow response to angiotensin-(1-7) in ovariectomized estradiol treated rats.
abstract: The angiotensin 1-7 (Ang 1-7), is abundantly produced in kidneys and antagonizes the function of angiotensin II through Mas receptor (MasR) or other unknown mechanisms. In the current study, the role of MasR and steroid hormone estrogen on renal blood flow response to Ang 1-7 administration was investigated in ovariectomized (OV) female rats. OV female Wistar-rats received estradiol (500 μg/kg/week) or vehicle for two weeks. In the day of the experiment, the animals were anesthetized, cannulated, and the responses including mean arterial pressure, renal blood flow (RBF), and renal vascular resistance at the constant level of renal perfusion pressure to graded infusion of Ang 1-7 at 0, 100 and 300 ng/kg/min were determined in OV and OV estradiol-treated (OVE) rats, treated with vehicle or MasR antagonist; A779. RBF response to Ang 1-7 infusion increased dose-dependently in vehicle (Pdose <0.001) and A779-treated (Pdose <0.01) animals. However, when MasR was blocked, the RBF response to Ang 1-7 significantly increased in OV animals compared with OVE rats (P<0.05). When estradiol was limited by ovariectomy, A779 increased RBF response to Ang 1-7 administration, while this response was attenuated in OVE animals.
authors.affiliation: Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, I.R. Iran; Department of Physiology, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
authors.forename: Shadan
authors.initials: S
authors.lastname: Saberi
authors.affiliation: Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, I.R. Iran; Department of Physiology, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
authors.forename: Aghdas
authors.initials: A
authors.lastname: Dehghani
authors.affiliation: Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, I.R. Iran; Department of Physiology, Isfahan University of Medical Sciences, Isfahan, I.R. Iran; IsfahanMN Institute of Basic and Applied Sciences Research, Isfahan, I.R. Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Nematbakhsh
keywords: Angiotensin 1-7; Estradiol; MasR; Renal blood flow