title: Day-restricted feeding during pregnancy and lactation programs glucose intolerance and impaired insulin secretion in male rat offspring.
abstract: The maternal environment during pregnancy and lactation plays a determining role in programming energy metabolism in offspring. Among a myriad of maternal factors, disruptions in the light/dark cycle during pregnancy can program glucose intolerance in offspring. Out-of-phase feeding has recently been reported to influence metabolism in adult humans and rodents; however, it is not known whether this environmental factor impacts offspring metabolism when applied during pregnancy and lactation. This study aims to determine whether maternal day-restricted feeding (DF) influences energy metabolism in offspring.
  
  
  
  Pregnant and lactating Wistar rats were subjected to ad libitum (AL) or DF during pregnancy and lactation. The offspring born to the AL and DF dams were intra- and interfostered, which resulted in 4 group types.
  
  
  
  The male offspring born to and breastfed by the DF dams (DF/DF off) were glucose intolerant, but without parallel insulin resistance as adults. Experiments with isolated pancreatic islets demonstrated that the male DF/DF off rats had reduced insulin secretion with no parallel disruption in calcium handling. However, this reduction in insulin secretion was accompanied by increased miRNA-29a and miRNA34a expression and decreased syntaxin 1a protein levels.
  
  
  
  We conclude that out-of-phase feeding during pregnancy and lactation can lead to glucose intolerance in male offspring, which is caused by a disruption in insulin secretion capacity. This metabolic programming is possibly caused by mechanisms dependent on miRNA modulation of syntaxin 1a.
authors.affiliation: Faculty of Medical Sciences, Department of Pharmacology, State University of Campinas, Campinas, Brazil.
authors.forename: J
authors.initials: J
authors.lastname: de Almeida Faria
authors.affiliation: Faculty of Medical Sciences, Department of Pharmacology, State University of Campinas, Campinas, Brazil.
authors.forename: T M F
authors.initials: TM
authors.lastname: de Araújo
authors.affiliation: Faculty of Medical Sciences, Department of Pharmacology, State University of Campinas, Campinas, Brazil.
authors.forename: R I
authors.initials: RI
authors.lastname: Mancuso
authors.affiliation: Faculty of Medical Sciences, Department of Pharmacology, State University of Campinas, Campinas, Brazil.
authors.forename: J
authors.initials: J
authors.lastname: Meulman
authors.affiliation: Faculty of Medical Sciences, Department of Pharmacology, State University of Campinas, Campinas, Brazil.
authors.forename: D
authors.initials: D
authors.lastname: da Silva Ferreira
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, Campinas, Brazil.
authors.forename: T M
authors.initials: TM
authors.lastname: Batista
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, Campinas, Brazil.
authors.forename: J F
authors.initials: JF
authors.lastname: Vettorazzi
authors.affiliation: Faculty of Medical Sciences, Department of Pharmacology, State University of Campinas, Campinas, Brazil.
authors.forename: P M R
authors.initials: PM
authors.lastname: da Silva
authors.affiliation: Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: S C
authors.initials: SC
authors.lastname: Rodrigues
authors.affiliation: Faculty of Medical Sciences, Department of Pharmacology, State University of Campinas, Campinas, Brazil.
authors.forename: A
authors.initials: A
authors.lastname: Kinote
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, Campinas, Brazil.
authors.forename: E M
authors.initials: EM
authors.lastname: Carneiro
authors.affiliation: Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: S
authors.initials: S
authors.lastname: Bordin
authors.affiliation: Faculty of Medical Sciences, Department of Pharmacology, State University of Campinas, Campinas, Brazil.
authors.forename: G F
authors.initials: GF
authors.lastname: Anhê
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D031204:Caloric Restriction / Q000009:adverse effects*; D004734:Energy Metabolism / Q000502:physiology; D005260:Female; D018149:Glucose Intolerance / Q000378:metabolism; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism; D007774:Lactation / Q000502:physiology*; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics; D009249:NADP / Q000378:metabolism; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D050827:Syntaxin 1 / Q000096:biosynthesis / Q000235:genetics
chemical_list: D007328:Insulin; C526366:MIRN29 microRNA, rat; C560944:MIRN34 microRNA, rat; D035683:MicroRNAs; C494213:Stx1a protein, rat; D050827:Syntaxin 1; D009249:NADP; D002118:Calcium
keywords: lactation; metabolic programming; out-of-phase feeding; pregnancy