title: Differential expression of microRNAs in bovine papillomavirus type 1 transformed equine cells.
abstract: Bovine papillomavirus (BPV) types 1 and 2 play an important role in the pathogenesis of equine sarcoids (ES), the most common cutaneous tumour affecting horses. MicroRNAs (miRNAs), small non-coding RNAs that regulate essential biological and cellular processes, have been found dysregulated in a wide range of tumours. The aim of this study was to identify miRNAs associated with ES. Differential expression of miRNAs was assessed in control equine fibroblasts (EqPalFs) and EqPalFs transformed with the BPV-1 genome (S6-2 cells). Using a commercially available miRNA microarray, 492 mature miRNAs were interrogated. In total, 206 mature miRNAs were differentially expressed in EqPalFs compared with S6-2 cells. Aberrant expression of these miRNAs in S6-2 cells can be attributed to the presence of BPV-1 genomes. Furthermore, we confirm the presence of 124 miRNAs previously computationally predicted in the horse. Our data supports the involvement of miRNAs in the pathogenesis of ES.
authors.affiliation: MRC - University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
authors.forename: N
authors.initials: N
authors.lastname: Terron-Canedo
authors.affiliation: Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
authors.forename: W
authors.initials: W
authors.lastname: Weir
authors.affiliation: School of Veterinary Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
authors.forename: L
authors.initials: L
authors.lastname: Nicolson
authors.affiliation: Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
authors.forename: C
authors.initials: C
authors.lastname: Britton
authors.affiliation: School of Veterinary Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
authors.forename: L
authors.initials: L
authors.lastname: Nasir
mesh_terms: D000818:Animals; D052688:Bovine papillomavirus 1; D002472:Cell Transformation, Viral; D005347:Fibroblasts / Q000378:metabolism / Q000821:virology; D006734:Horse Diseases / Q000378:metabolism; D006736:Horses; D035683:MicroRNAs / Q000378:metabolism*; D030361:Papillomavirus Infections / Q000378:metabolism / Q000662:veterinary* / Q000821:virology; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D012878:Skin Neoplasms / Q000378:metabolism / Q000662:veterinary / Q000821:virology
chemical_list: D035683:MicroRNAs
keywords: bovine papillomavirus; equine fibroblasts; equine sarcoid; miRNA; microRNA; microarray