title: Identification and characterization of the melanoma differentiation - associated gene 5 in sea perch, Lateolabrax japonicus.
abstract: The RIG-I-like receptors family is a group of cytosolic RNA helicase proteins that can recognize viral RNA via binding to pathogen associated molecular pattern motifs within RNA ligands. A novel vertebrate RLR counterpart named LjMDA5 was firstly identified from the marine fish sea perch Lateolabrax japonicus in this study. The full-length cDNA of LjMDA5 is 3750 bp and encodes a polypeptide of 988 amino acids, containing two N-terminal tandem caspase activation and recruitment domains, a DExH (Asp-Glu-X-His) box domain, an HELICc domain, and a C-terminal domain RIG-I. Phylogenetic analysis showed that LjMDA5 shared the closest genetic relationship with the MDA5 of Larimichthys crocea. Quantitative RT-PCR analysis showed that LjMDA5 was ubiquitously expressed and up-regulated significantly in all selected tissues in vivo post NNV infection. Time course analysis showed that LjMDA5 transcripts significantly increased in spleen and kidney. We found LjMDA5 could be regulated in the sea perch LJB and LJF cell lines after lipopolysaccharide, polyinosinic-polycytidylic acid treatment and NNV challenge. RNA interference experiment indicated that silencing of LjMDA5 significantly increased RGNNV replication and virus production in NNV infected LJF cells. Our results revealed that MDA5 was essential for host defense against NNV, which provided new insights into the function of RLR signaling pathway during NNV infection in fish.
authors.affiliation: Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Zhuhai Key Laboratory of Marine Bioresources and Environment, School of Marine Sciences, Sun Yat-sen University, Guangzhou, China. Electronic address: jiapeng19890219@126.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Jia
authors.affiliation: Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Zhuhai Key Laboratory of Marine Bioresources and Environment, School of Marine Sciences, Sun Yat-sen University, Guangzhou, China. Electronic address: jiakuntong@126.com.
authors.forename: Kuntong
authors.initials: K
authors.lastname: Jia
authors.affiliation: Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Zhuhai Key Laboratory of Marine Bioresources and Environment, School of Marine Sciences, Sun Yat-sen University, Guangzhou, China. Electronic address: 2562492896@qq.com.
authors.forename: Limin
authors.initials: L
authors.lastname: Chen
authors.affiliation: Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Zhuhai Key Laboratory of Marine Bioresources and Environment, School of Marine Sciences, Sun Yat-sen University, Guangzhou, China. Electronic address: 916107751@qq.com.
authors.forename: Yao
authors.initials: Y
authors.lastname: Le
authors.affiliation: Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Zhuhai Key Laboratory of Marine Bioresources and Environment, School of Marine Sciences, Sun Yat-sen University, Guangzhou, China. Electronic address: 369277368@qq.com.
authors.forename: Yilin
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Zhuhai Key Laboratory of Marine Bioresources and Environment, School of Marine Sciences, Sun Yat-sen University, Guangzhou, China. Electronic address: zhangj358@mail2.sysu.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China. Electronic address: zhulimeimlz@163.com.
authors.forename: Limei
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China. Electronic address: zhangli@scsio.ac.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Zhuhai Key Laboratory of Marine Bioresources and Environment, School of Marine Sciences, Sun Yat-sen University, Guangzhou, China. Electronic address: yimsh@mail.sysu.edu.cn.
authors.forename: Meisheng
authors.initials: M
authors.lastname: Yi
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular; D005393:Fish Diseases / Q000276:immunology*; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D000072640:Interferon-Induced Helicase, IFIH1 / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D030381:Nodaviridae / Q000276:immunology*; D010471:Perches / Q000276:immunology*; D010802:Phylogeny; D012327:RNA Virus Infections / Q000276:immunology*; D034741:RNA, Small Interfering / Q000235:genetics; D013154:Spleen / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D029941:Fish Proteins; D034741:RNA, Small Interfering; D000072640:Interferon-Induced Helicase, IFIH1
keywords: Innate immune; Melanoma differentiation-associated gene 5; Nervous necrosis virus; Sea perch