title: Polycaprolactone-laponite composite scaffold releasing strontium ranelate for bone tissue engineering applications.
abstract: We report polycaprolactone-laponite composite scaffold for the controlled release of strontium ranelate (SRA), a drug for osteoporosis. Laponite-SRA complex with electrostatic interaction between the drug and laponite was obtained through an aqueous phase reaction. Structural evaluation verified complexation of the bulky SRA molecules with the negatively charged laponite tactoid surfaces, leading to extended ordering of the tactoids, leaving behind the interlayer spacing of the laponite unchanged. The laponite-SRA complex was solution blended with polycaprolactone to obtain composite scaffolds. The strategy was found improving the dispersibility of laponite in PCL due to partial organomodification imparted through interaction with the SRA. The composite scaffolds with varying laponite-SRA complex content of 3-12wt% were evaluated in vitro using human osteosarcoma cells. It was confirmed that an optimum composition of the scaffold with 3wt% laponite-SRA complex loading would be ideal for obtaining enhanced ALP activity, by maintaining cell viability.
authors.affiliation: Division of Tissue Engineering and Regeneration Technologies, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram 695012, Kerala, India; Department of Chemistry, Mahatma Gandhi College, Thiruvananthapuram 695004, Kerala, India. Electronic address: bindumelekkuttu@gmail.com.
authors.forename: Bindu P
authors.initials: BP
authors.lastname: Nair
authors.affiliation: Division of Tissue Engineering and Regeneration Technologies, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram 695012, Kerala, India.
authors.forename: Megha
authors.initials: M
authors.lastname: Sindhu
authors.affiliation: Division of Tissue Engineering and Regeneration Technologies, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram 695012, Kerala, India. Electronic address: prabha@sctimst.ac.in.
authors.forename: Prabha D
authors.initials: PD
authors.lastname: Nair
mesh_terms: D050071:Bone Density Conservation Agents / Q000737:chemistry* / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003692:Delayed-Action Preparations / Q000737:chemistry* / Q000494:pharmacology; D004339:Drug Compounding; D065546:Drug Liberation; D006801:Humans; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D011091:Polyesters / Q000737:chemistry*; D017640:Silicates / Q000737:chemistry*; D013876:Thiophenes / Q000737:chemistry* / Q000494:pharmacology; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D050071:Bone Density Conservation Agents; D003692:Delayed-Action Preparations; D011091:Polyesters; D017640:Silicates; D013876:Thiophenes; C081587:strontium ranelate; C016240:polycaprolactone; C524813:laponite
keywords: Bone tissue engineering; Clay; Laponite; Osteoporosis; Strontium ranelate