title: [Effect of specific sequence oligodeoxynucleotide MT01 on the proliferation, apoptosis, and cell cycle of osteoblasts invaded by Porphyromonas gingivalis].
abstract: OBJECTIVE
  This aimed to investigate the effect of specific sequence oligodeoxynucleotide MT01 on the biological properties of osteoblasts invaded by Porphyromonas gingivalis (P. gingivalis ) by evaluating proliferation, cell cycle, and apoptosis.
  
  
  METHODS
  MG63 osteoblasts were recovered and incubated with MT01, CpG ODN, metronidazole (MNZ), and gentamicin (GEN) for 3 h. P. gingivalis (the multiplicity of infection was 100:1) was added subsequently and cocultured for another 24 and 48 h. Cells with PBS comprised the blank group, whereas cells with P. gingivalis comprised the negative controls. Six experimental groups were established: PBS group, P. gingivalis group, MT01+P. gingivalis group, CpG ODN+ P. gingivalis group, MNZ+P. gingivalis group, and GEN+P. gingivalis group. The proliferative ability was measured by methyl thiazolyl tetrazolium assay, and the percentages of apoptosis and cell cycle were examined by flow cytometry.
  
  
  RESULTS
  Compared with the blank group, proliferation increased significantly in the MT01+P. gingivalis group (P < 0.05). The ratio of cells was lower at the G₁ phase and higher at the S phase in the MT01+P. gingivalis group compared with the results in the P. gingivalis group (P < 0.05). Early cell apoptosis in the MT01+P. gingivalis group was significantly lower than that in the P. gingivalis group (P < 0.05).
  
  
  CONCLUSIONS
  MT01 can promote the proliferation, reduce the ratio of the G₁phase, increase the ratio of the S phase, and inhibit the early apoptosis of osteoblasts invaded by P. gingivalis.
authors.forename: Yu
authors.initials: Y
authors.lastname: Haijiao
authors.forename: Shen
authors.initials: S
authors.lastname: Yuqin
authors.forename: Liu
authors.initials: L
authors.lastname: Yin
authors.forename: Gao
authors.initials: G
authors.lastname: Han
authors.forename: Zhou
authors.initials: Z
authors.lastname: Yue
authors.forename: Hu
authors.initials: H
authors.lastname: Tianqi
authors.forename: Lin
authors.initials: L
authors.lastname: Chongtao
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division; D049109:Cell Proliferation / Q000187:drug effects*; D005434:Flow Cytometry; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D008795:Metronidazole / Q000494:pharmacology; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D016966:Porphyromonas gingivalis / Q000472:pathogenicity*
chemical_list: C408982:CPG-oligonucleotide; D005839:Gentamicins; D009838:Oligodeoxyribonucleotides; D008795:Metronidazole