title: Numerical modelling of biophysicochemical effects on multispecies reactive transport in porous media involving Pseudomonas putida for potential microbial enhanced oil recovery application.
abstract: pH and resident time of injected slug plays a critical role in characterizing the reservoir for potential microbial enhanced oil recovery (MEOR) application. To investigate MEOR processes, a multispecies (microbes-nutrients) reactive transport model in porous media was developed by coupling kinetic and transport model. The present work differs from earlier works by explicitly determining parametric values required for kinetic model by experimental investigations using Pseudomonas putida at different pH conditions and subsequently performing sensitivity analysis of pH, resident time and water saturation on concentrations of microbes, nutrients and biosurfactant within reservoir. The results suggest that nutrient utilization and biosurfactant production are found to be maximum at pH 8 and 7.5 respectively. It is also found that the sucrose and biosurfactant concentrations are highly sensitive to pH rather than reservoir microbial concentration, while at larger resident time and water saturation, the microbial and nutrient concentrations were lesser due to enhanced dispersion.
authors.affiliation: Petroleum Engineering Program, Department of Ocean Engineering, Indian Institute of Technology-Madras, Chennai 600036, India. Electronic address: sivasankarpetro@gmail.com.
authors.forename: P
authors.initials: P
authors.lastname: Sivasankar
authors.affiliation: Petroleum Engineering Program, Department of Ocean Engineering, Indian Institute of Technology-Madras, Chennai 600036, India. Electronic address: arkanna1983@gmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Rajesh Kanna
authors.affiliation: Petroleum Engineering Program, Department of Ocean Engineering, Indian Institute of Technology-Madras, Chennai 600036, India. Electronic address: gskumar@iitm.ac.in.
authors.forename: G
authors.initials: G
authors.lastname: Suresh Kumar
authors.affiliation: Applied and Industrial Microbiological Laboratory, Department of Biotechnology, Bhupat and Jyothi Mehta School of Biosciences, Indian Institute of Technology-Madras, Chennai 600036, India. Electronic address: gummadi@iitm.ac.in.
authors.forename: Sathyanarayana N
authors.initials: SN
authors.lastname: Gummadi
mesh_terms: D056804:Biofuels; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008962:Models, Theoretical; D016958:Pseudomonas putida / Q000254:growth & development / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D056804:Biofuels; D003470:Culture Media; D013501:Surface-Active Agents; D013395:Sucrose
keywords: Microbial enhanced oil recovery; Porous medium; Pseudomonas putida; Reactive transport modelling; pH