title: Ultrastructure of a Mobile Threadlike Tissue Floating in a Lymph Vessel.
abstract: Observations of the primo vascular system (PVS) floating in lymph ducts were reported by various groups. There have been, however, no studies on the ultrastructure of the entire cross section of a primo vessel (PV) inside a lymph vessel with a transmission electron microscope (TEM). In the current study we took the TEM images of a cross section of the PV inside a lymph vessel. We used the Alcian blue staining method for the finding of the target PV in a lymphatic vessel by injecting the dye into the inguinal lymph nodes. The stained PV was harvested together with the lymph vessel and some parts of the specimens were used for studying with optical microscopes. Some other parts were treated according to a standard protocol for TEM. As the results the TEM study revealed the loosely distributed collagen fibers with plenty of empty spaces and the lumens with the endothelial nuclei. It turned out to be very similar to the ultrastructure of the PVs observed on the surfaces of internal organs. It also showed how compactly the PV is surrounded with lymphocytes. In conclusion, the detailed morphological features like the distribution of fibers in the PV were revealed and shown to be similar to another kind of the PV on the surfaces of internal organs.
authors.affiliation: Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon 443-270, Republic of Korea; Department of Transdisciplinary Studies, Graduate School of Convergence Science and Technology, Seoul National University, Suwon 443-270, Republic of Korea.
authors.forename: Sharon Jiyoon
authors.initials: SJ
authors.lastname: Jung
authors.affiliation: Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon 443-270, Republic of Korea.
authors.forename: HyunJi
authors.initials: H
authors.lastname: Gil
authors.affiliation: Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon 443-270, Republic of Korea.
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Integrative Research Support Center, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea.
authors.forename: Hong-Lim
authors.initials: HL
authors.lastname: Kim
authors.affiliation: Department of Acupuncture & Moxibustion, Wonkwang University, Gwangju Medical Hospital, Gwangju 503-310, Republic of Korea.
authors.forename: Sungchul
authors.identifier: 0000-0001-9274-9243
authors.initials: S
authors.lastname: Kim
authors.affiliation: Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon 443-270, Republic of Korea.
authors.forename: Kwang-Sup
authors.identifier: 0000-0001-8776-7877
authors.initials: KS
authors.lastname: Soh