title: Raft-based interactions of gangliosides with a GPI-anchored receptor.
abstract: Gangliosides, glycosphingolipids containing one or more sialic acid(s) in the glyco-chain, are involved in various important physiological and pathological processes in the plasma membrane. However, their exact functions are poorly understood, primarily because of the scarcity of suitable fluorescent ganglioside analogs. Here, we developed methods for systematically synthesizing analogs that behave like their native counterparts in regard to partitioning into raft-related membrane domains or preparations. Single-fluorescent-molecule imaging in the live-cell plasma membrane revealed the clear but transient colocalization and codiffusion of fluorescent ganglioside analogs with a fluorescently labeled glycosylphosphatidylinisotol (GPI)-anchored protein, human CD59, with lifetimes of 12 ms for CD59 monomers, 40 ms for CD59's transient homodimer rafts in quiescent cells, and 48 ms for engaged-CD59-cluster rafts, in cholesterol- and GPI-anchoring-dependent manners. The ganglioside molecules were always mobile in quiescent cells. These results show that gangliosides continually and dynamically exchange between raft domains and the bulk domain, indicating that raft domains are dynamic entities.
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, Japan.|Department of Applied Bioorganic Chemistry, Gifu University, Gifu, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Komura
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, Japan.|The Institute for Stem Cell Biology and Regenerative Medicine (inStem), The National Centre for Biological Sciences (NCBS), Bangalore, India.
authors.forename: Kenichi G N
authors.initials: KG
authors.lastname: Suzuki
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, Japan.|Department of Applied Bioorganic Chemistry, Gifu University, Gifu, Japan.
authors.forename: Hiromune
authors.initials: H
authors.lastname: Ando
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, Japan.|Department of Applied Bioorganic Chemistry, Gifu University, Gifu, Japan.
authors.forename: Miku
authors.initials: M
authors.lastname: Konishi
authors.affiliation: Department of Applied Bioorganic Chemistry, Gifu University, Gifu, Japan.
authors.forename: Machi
authors.initials: M
authors.lastname: Koikeda
authors.affiliation: Department of Applied Bioorganic Chemistry, Gifu University, Gifu, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Imamura
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, Japan.
authors.forename: Rahul
authors.initials: R
authors.lastname: Chadda
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, Japan.
authors.forename: Takahiro K
authors.initials: TK
authors.lastname: Fujiwara
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, Japan.
authors.forename: Hisae
authors.initials: H
authors.lastname: Tsuboi
authors.affiliation: Department of Chemistry, University of Illinois at Chicago, Chicago, Illinois, USA.
authors.forename: Ren
authors.initials: R
authors.lastname: Sheng
authors.affiliation: Department of Chemistry, University of Illinois at Chicago, Chicago, Illinois, USA.
authors.forename: Wonhwa
authors.initials: W
authors.lastname: Cho
authors.affiliation: Department of Biochemistry II, Nagoya University, Nagoya, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Furukawa
authors.affiliation: Department of Life and Medical Sciences, Chubu University, Nagoya, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Furukawa
authors.affiliation: Department of Biochemistry II, Nagoya University, Nagoya, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Yamauchi
authors.affiliation: Department of Applied Bioorganic Chemistry, Gifu University, Gifu, Japan.
authors.forename: Hideharu
authors.initials: H
authors.lastname: Ishida
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, Japan.|Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan.|Membrane Cooperativity Unit, Okinawa Institute of Science and Technology, Onna-son, Okinawa, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Kusumi
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, Japan.|Department of Applied Bioorganic Chemistry, Gifu University, Gifu, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kiso
mesh_terms: D018957:CD59 Antigens / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D004058:Diffusion; D005453:Fluorescence; D005732:Gangliosides / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D017261:Glycosylphosphatidylinositols / Q000378:metabolism*; D006801:Humans; D021962:Membrane Microdomains / Q000737:chemistry / Q000378:metabolism*; D008968:Molecular Conformation; D011485:Protein Binding; D013997:Time Factors
chemical_list: D018957:CD59 Antigens; D005732:Gangliosides; D017261:Glycosylphosphatidylinositols; C051590:CD59 protein, human