title: Histologic features of acute thrombi retrieved from stroke patients during mechanical reperfusion therapy.
abstract: The histologic features of thrombus may differ according to the stroke subtypes. However, in acute reperfusion therapy, fibrin-specific thrombolytics are used based on the assumption that all thrombi are alike.
  
  
  
  The histologic characteristics of thrombi were compared between patients with different stroke etiologies.
  
  
  
  Between April 2010 and March 2012, we analyzed thrombi retrieved from acute stroke patients during mechanical thrombectomy. All thrombi were analyzed using component-specific stains such as Martius scarlet blue for fibrins and immunostaining with CD42b antibody for platelets. The stroke subtypes were determined based on the Trial of ORG 10172 in Acute Stroke Treatment classification.
  
  
  
  Among 36 patients, 22 were diagnosed with cardioembolism, 8 with atherothrombosis, and 6 with undetermined etiology. In arteriogenic thrombi, red blood cells were most abundant (56.9 ± 12.2%), and the platelets covered the fibrin layers or were localized at the edge or periphery of the thrombus. In cardiogenic thrombi, fibrin was most abundant (39.5 ± 13.5%), and platelets were clustered within the rich fibrin. Red blood cells proportion was greater in arteriogenic thrombi than in cardiogenic thrombi (p < 0.001), whereas fibrin proportion was greater in cardiogenic thrombi than in arteriogenic thrombi (p = 0.003). Of six patients with undetermined etiology, the thrombi in five showed histologic features and composition similar to that of cardiogenic thrombi.
  
  
  
  Acute thrombi showed different histologic features according to the stroke etiology. The distribution of platelets and proportion of red blood cells and fibrin were major distinguishing factors between stroke subtypes.
authors.affiliation: Department of Neurology, Chosun University School of Medicine, GwangJu, Republic of Korea shahn@chosun.ac.kr.
authors.forename: Seong Hwan
authors.initials: SH
authors.lastname: Ahn
authors.affiliation: Department of Pathology, Chosun University School of Medicine, GwangJu, Republic of Korea.
authors.forename: Ran
authors.initials: R
authors.lastname: Hong
authors.affiliation: Department of Neurology, Chosun University School of Medicine, GwangJu, Republic of Korea.
authors.forename: In Sung
authors.initials: IS
authors.lastname: Choo
authors.affiliation: Department of Neurology, Yonsei University College of Medicine, Seoul, Republic of Korea.
authors.forename: Ji Hoe
authors.initials: JH
authors.lastname: Heo
authors.affiliation: Department of Neurology, Yonsei University College of Medicine, Seoul, Republic of Korea.
authors.forename: Hyo Suk
authors.initials: HS
authors.lastname: Nam
authors.affiliation: Department of Neurology, Chosun University School of Medicine, GwangJu, Republic of Korea.
authors.forename: Hyun Goo
authors.initials: HG
authors.lastname: Kang
authors.affiliation: Department of Neurology, Chosun University School of Medicine, GwangJu, Republic of Korea.
authors.forename: Hoo Won
authors.initials: HW
authors.lastname: Kim
authors.affiliation: Department of Neurology, Chosun University School of Medicine, GwangJu, Republic of Korea.
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Kim
mesh_terms: D000368:Aged; D001792:Blood Platelets / Q000378:metabolism / Q000473:pathology; D002548:Cerebral Revascularization; D004912:Erythrocytes / Q000378:metabolism / Q000473:pathology; D005260:Female; D005337:Fibrin / Q000378:metabolism; D006801:Humans; D008297:Male; D061185:Mechanical Thrombolysis; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000378:metabolism; D015424:Reperfusion; D020521:Stroke / Q000209:etiology* / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D013927:Thrombosis / Q000209:etiology / Q000378:metabolism / Q000473:pathology* / Q000601:surgery
chemical_list: D019038:Platelet Glycoprotein GPIb-IX Complex; D005337:Fibrin
keywords: Ischemic stroke; etiology; histology; stroke subtype; thrombectomy; thrombus