title: Streptococcus uberis ST439 and ST475 induce differential inflammatory responses in a mouse intramammary infection model.
abstract: Streptococcus uberis causing mastitis is a growing challenge to the dairy industry. Molecular, epidemiological and population structure studies have revealed clonal diversity among the infecting strains. In this study, mouse intramammary infection model was used to uncover the host immune response to two epidemiologically important live strains of S. uberis (SU1and SU2) obtained from subclinical case of mastitis possessing specific and unique multi locus sequence types (ST), pulsed field gel electrophoresis (PFGE) pulsotypes and virulence profiles. Temporal (2h, 4h, 8h, 12h, 24h and 48h) expression of key inflammatory mediators (IL2, IL4, IL6, IL12, TNFα, IFNγ, GMCSF, TLR2, TLR4, TLR9, TLR11, TLR12, CD14, IL1β, RANTES, Lactoferrin, and CXCl1) by reverse transcription and probe-based quantitative real-time PCR showed relative mRNA levels higher (p<0.05) in response to SU2 compared with SU1 with 24h PI serving as a critical point for the deviating behavior (SU1 versus SU2). Further employing the predicted biological processes under the influence of this pool of tested genes, the delineation of gene regulatory networks suggested SU1-favoring its persistence in the host environment; in contrast, SU2-which elevated gene expression indicating towards pathogen clearance or immune surveillance. This study suggested how these unique strains could manipulate the host immune response to influence the severity of mastitis; our results expand the available information on host pathogen interaction and provide a firm foundation needing further investigations to gain control over this pathogen.
authors.affiliation: ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Ramagondanahalli, Yelahanka, Bengaluru, Karnataka, India; Department of Biotechnology, Assam University, Silchar, Assam, India.
authors.forename: Susweta Das
authors.initials: SD
authors.lastname: Mitra
authors.affiliation: ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Ramagondanahalli, Yelahanka, Bengaluru, Karnataka, India. Electronic address: brshome@gmail.com.
authors.forename: Bibek Ranjan
authors.initials: BR
authors.lastname: Shome
authors.affiliation: ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Ramagondanahalli, Yelahanka, Bengaluru, Karnataka, India.
authors.forename: Bhuvana
authors.initials: B
authors.lastname: Mani
authors.affiliation: ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Ramagondanahalli, Yelahanka, Bengaluru, Karnataka, India.
authors.forename: D
authors.initials: D
authors.lastname: Velu
authors.affiliation: ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Ramagondanahalli, Yelahanka, Bengaluru, Karnataka, India.
authors.forename: Apala
authors.initials: A
authors.lastname: Banerjee
authors.affiliation: BionividPvt Ltd, Kasturinagar, Bengaluru, Karnataka, India.
authors.forename: Kiran
authors.initials: K
authors.lastname: Bankar
authors.affiliation: Department of Biotechnology, Assam University, Silchar, Assam, India.
authors.forename: Sankar Kumar
authors.initials: SK
authors.lastname: Ghosh
authors.affiliation: ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Ramagondanahalli, Yelahanka, Bengaluru, Karnataka, India.
authors.forename: Sandip
authors.initials: S
authors.lastname: Santra
authors.affiliation: ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Ramagondanahalli, Yelahanka, Bengaluru, Karnataka, India.
authors.forename: K P
authors.initials: KP
authors.lastname: Suresh
authors.affiliation: ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Ramagondanahalli, Yelahanka, Bengaluru, Karnataka, India.
authors.forename: Habibur
authors.initials: H
authors.lastname: Rahman
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D053263:Gene Regulatory Networks; D018836:Inflammation Mediators / Q000378:metabolism; D008413:Mastitis / Q000235:genetics / Q000382:microbiology* / Q000503:physiopathology; D051379:Mice; D011247:Pregnancy; D013291:Streptococcus / Q000472:pathogenicity*
chemical_list: D018836:Inflammation Mediators
keywords: Immune response; Mastitis; Mice model; S. uberis