title: Analysis of the Expression and Function of Immunoglobulin-Like Transcript 4 (ILT4, LILRB2) in Dendritic Cells from Patients with Systemic Lupus Erythematosus.
abstract: Dendritic cells (DC) play an important role in the development and maintenance of immune tolerance. Although the inhibitory receptor ILT4/LILRB2 has been related with the tolerogenic phenotype of DC, the possible role of this receptor in the breakdown of DC tolerogenic function in systemic lupus erythematosus (SLE) has not been elucidated. In this study, we analyzed the expression and function of the inhibitory receptor ILT4 in DC from SLE patients. We found that the percentage of ILT4 positive plasmacytoid DC and myeloid DC is significantly diminished in SLE patients. Interestingly, ligation of ILT4 did not affect the maturation or immunogenic capability of DC in healthy controls. In contrast, in SLE patients we observed an inhibitory effect of ILT4 on the immunogenic capability of DC. ILT4 was shown not to have a crucial role in regulating the maturation and function of DC from healthy controls but is partially involved in the maturation process and immunogenic capability of DC from SLE patients, suggesting that other inhibitory receptors, involved in the regulation of DC tolerogenic function, may be impaired in this autoimmune disease.
authors.affiliation: Departamento de Inmunología, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, Avenida Venustiano Carranza 2405, 78210 San Luis Potosí, SLP, Mexico.
authors.forename: Paola Del Carmen
authors.initials: Pdel C
authors.lastname: Guerra-de Blas
authors.affiliation: Departamento de Inmunología, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, Avenida Venustiano Carranza 2405, 78210 San Luis Potosí, SLP, Mexico.
authors.forename: Yael Sebastián
authors.initials: YS
authors.lastname: Villaseñor-Talavera
authors.affiliation: Departamento de Inmunología, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, Avenida Venustiano Carranza 2405, 78210 San Luis Potosí, SLP, Mexico.
authors.forename: Daniela de Jesús
authors.initials: Dde J
authors.lastname: Cruz-González
authors.affiliation: Departamento de Inmunología, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, Avenida Venustiano Carranza 2405, 78210 San Luis Potosí, SLP, Mexico; Departamento de Reumatología, Hospital Central "Dr. Ignacio Morones Prieto", Avenida Venustiano Carranza 2395, 78290 San Luis Potosí, SLP, Mexico.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Baranda
authors.affiliation: Departamento de Inmunología, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, Avenida Venustiano Carranza 2405, 78210 San Luis Potosí, SLP, Mexico.
authors.forename: Lesly
authors.initials: L
authors.lastname: Doníz-Padilla
authors.affiliation: Departamento de Reumatología, Hospital Central "Dr. Ignacio Morones Prieto", Avenida Venustiano Carranza 2395, 78290 San Luis Potosí, SLP, Mexico.
authors.forename: Carlos
authors.initials: C
authors.lastname: Abud-Mendoza
authors.affiliation: Departamento de Inmunología, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, Avenida Venustiano Carranza 2405, 78210 San Luis Potosí, SLP, Mexico.
authors.forename: Roberto
authors.initials: R
authors.lastname: González-Amaro
authors.affiliation: Departamento de Inmunología, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, Avenida Venustiano Carranza 2405, 78210 San Luis Potosí, SLP, Mexico.
authors.forename: Adriana Elizabeth
authors.initials: AE
authors.lastname: Monsiváis-Urenda
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006801:Humans; D007108:Immune Tolerance; D000075362:Leukocyte Immunoglobulin-like Receptor B1; D008070:Lipopolysaccharides / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D010641:Phenotype; D011971:Receptors, Immunologic / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; D016207:Cytokines; C105299:LILRB1 protein, human; C491341:LILRB2 protein, human; D000075362:Leukocyte Immunoglobulin-like Receptor B1; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic