title: Biological and Chemical Characterization of a New Larvicide Ovitrap Made of Plastic With Pyriproxyfen Incorporated for Aedes aegypti (Diptera: Culicidae) Control.
abstract: Aedes aegypti (L.) is a species of international concern because of its ability to transmit serious human arboviral diseases including yellow fever, dengue, and chikungunya, which have spread to all continents. Ovitraps are containers constructed to imitate Aedes' natural breeding sites and have been used for many decades as a sensitive and inexpensive surveillance tool for detecting the presence of container-inhabiting mosquitoes. In addition to their value for vector surveillance, various ovitrap devices have been evaluated as tools for suppressing Ae. aegypti populations. In this study, we performed a biological and chemical characterization of a new ovitrap prototype manufactured by injection molding of low-density polyethylene (LDPE) with the larvicide pyriproxyfen. Our research shows that pyriproxyfen was immediately released from the LDPE into the water of the ovitrap and led to an emergence inhibition of 100% for over 30 weeks. In addition, ovitraps continued to show a high larvicidal activity after over 20 washes. Pyriproxyfen was detectable in the water after 20 s and reached a peak after 24 h. Our results show that this ovitrap can be an effective, inexpensive, and low-maintenance tool for Ae. aegypti surveillance and control.
authors.affiliation: Centro de Investigaciones de Plagas e Insecticidas (CIPEIN-UNIDEF/CITEDEF/CONICET), J.B. de LaSalle 4397, Villa Martelli (1603), Buenos Aires, Argentina (lharburguer@citedef.gob.ar; slicastro@citedef.gob.ar; hmasuh@citedef.gob.ar; ezerba@citedef.gob.ar), hmasuh@citedef.gob.ar.
authors.forename: Laura
authors.initials: L
authors.lastname: Harburguer
authors.affiliation: Centro de Investigaciones de Plagas e Insecticidas (CIPEIN-UNIDEF/CITEDEF/CONICET), J.B. de LaSalle 4397, Villa Martelli (1603), Buenos Aires, Argentina (lharburguer@citedef.gob.ar; slicastro@citedef.gob.ar; hmasuh@citedef.gob.ar; ezerba@citedef.gob.ar).
authors.forename: Susana
authors.initials: S
authors.lastname: Licastro
authors.affiliation: Centro de Investigaciones de Plagas e Insecticidas (CIPEIN-UNIDEF/CITEDEF/CONICET), J.B. de LaSalle 4397, Villa Martelli (1603), Buenos Aires, Argentina (lharburguer@citedef.gob.ar; slicastro@citedef.gob.ar; hmasuh@citedef.gob.ar; ezerba@citedef.gob.ar), hmasuh@citedef.gob.ar.|Instituto de Investigaciones e Ingeniería Ambiental (3IA), Universidad Nacional de San Martín, San Martín, Buenos Aires, Argentina.
authors.forename: Héctor
authors.initials: H
authors.lastname: Masuh
authors.affiliation: Centro de Investigaciones de Plagas e Insecticidas (CIPEIN-UNIDEF/CITEDEF/CONICET), J.B. de LaSalle 4397, Villa Martelli (1603), Buenos Aires, Argentina (lharburguer@citedef.gob.ar; slicastro@citedef.gob.ar; hmasuh@citedef.gob.ar; ezerba@citedef.gob.ar).|Instituto de Investigaciones e Ingeniería Ambiental (3IA), Universidad Nacional de San Martín, San Martín, Buenos Aires, Argentina.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Zerba
mesh_terms: D000330:Aedes / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D000818:Animals; D007303:Insect Vectors / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D007306:Insecticides / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D009032:Mosquito Control / Q000295:instrumentation / Q000379:methods*; D011725:Pyridines / Q000494:pharmacology*
chemical_list: D007306:Insecticides; D011725:Pyridines; C055613:pyriproxyfen
keywords: Aedes aegypti; larvicide; low-density polyethylene; ovitrap; pyriproxyfen