title: Identification and characterization of in vivo metabolites of asulacrine using advanced mass spectrophotometry technique in combination with improved data mining strategy.
abstract: Asulacrine (ASL) is a broad-spectrum, antitumor drug whose data are promising for the treatment of breast and lung cancers; however, a high incidence of phlebitis hampered its further development. Phlebitis is associated with generation of reactive species. Asulacrine donates electrons and produces oxidative stress in chemical reactions. It was expected that ASL would actively metabolize to oxidized products through reactive intermediates and produce more products in vivo than reported and thus cause phlebitis. A comprehensive study was planned to investigate in vivo metabolism of ASL, using high-resolution mass spectrometry LC/IT-TOF MS in positive mode. Metabolites were detected by different software by applying annotated detection strategy. The possible metabolites and their product ions were simultaneously detected by segmented data acquisition to get accurate mass values. Segmented data acquisition improved signal-to-noise (S/N) ratio, which was helpful to detect metabolites and their fragments even when present in trace amounts. A total of 21 metabolites were detected in gender-based biological fluids and characterized by comparing their accurate mass values, fragmentation patterns, and relative retention times with that of ASL. Among previously reported glucuronosylation metabolites, some oxidation, hydroxylation, carboxylation, demethylation, hydrogenation, glutamination, and acetylcysteine conjugation were detected for the first time. Twenty metabolites were tentatively identified by using the annotated strategy for data acquisition and post-data mining.
authors.affiliation: Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University (CPU), Nanjing, China.
authors.forename: Attia
authors.initials: A
authors.lastname: Afzal
authors.affiliation: Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University (CPU), Nanjing, China.
authors.forename: Yunxi
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: School of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology (HUST), Wuhan, China.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Sarfraz
authors.affiliation: Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University (CPU), Nanjing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University (CPU), Nanjing, China.
authors.forename: Longsheng
authors.initials: L
authors.lastname: Sheng
authors.affiliation: School of Pharmacy, The University of Auckland, New Zealand.
authors.forename: Zimei
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University (CPU), Nanjing, China. Electronic address: jgsun@cpu.edu.cn.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Sun
authors.affiliation: Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University (CPU), Nanjing, China. Electronic address: guangjiwang@hotmail.com.
authors.forename: Guangji
authors.initials: G
authors.lastname: Wang
mesh_terms: D000677:Amsacrine / Q000031:analogs & derivatives* / Q000378:metabolism; D000818:Animals; D001646:Bile / Q000737:chemistry; D057225:Data Mining; D005260:Female; D008297:Male; D013058:Mass Spectrometry; D008970:Molecular Weight; D051381:Rats; D012984:Software; D014556:Urine / Q000737:chemistry
chemical_list: D000677:Amsacrine; C042315:asulacrine
keywords: Asulacrine; LC/IT-TOF/MS; Metabolite characterization; Post-acquisition data mining; Segmented data acquisition