title: Photobiomodulation of Dental Derived Mesenchymal Stem Cells: A Systematic Review.
abstract: OBJECTIVE
  This study aimed to conduct a systematic review of the literature published from 2000 to August 2015, to investigate the effect of photobiomodulation (PBM) therapy on dentoalveolar-derived mesenchymal stem cells (ddMSCs), assessing whether a clear conclusion can be reached from the data presented.
  
  
  BACKGROUND
  Systematic reviews provide the best evidence on the effectiveness of a procedure and permit investigation of factors that may influence the performance of a method. To the best of our knowledge, no previous systematic review has evaluated the effects of PBM only on ddMSCs.
  
  
  METHODS
  The search was conducted in PubMed /MEDLINE®, Scopus and Web of Science databases, and reported according to the Preferred Reporting Items for Systematic Reviews and Metaanalyses (PRISMA Statement). Original research articles investigating the effects of PBM therapy on ddMSCs, published from 2000 to August 2015, were retrieved and used for this review according to the following eligibility criteria: evaluating PBM therapy, assessing stem cells of dentoalveolar origin, published in English, dealing with cells characterized as stem cells, and using light that did not need external chromophores.
  
  
  RESULTS
  From the initial 3467 potentially relevant articles identified, 6 were excluded because they were duplicates, and 3453 were considered ineligible based on the inclusion criteria. Therefore, eight articles remained, and these were fully analyzed in order to closely check exclusion criteria items. Only one of them was excluded because the cultured cells studied were not characterized as stem cells. Finally, seven articles served as the basis for this systematic review.
  
  
  CONCLUSIONS
  PBM therapy has no deleterious effects on ddMSCs. Although no other clear conclusion was obtained because of the scarce number of publications, the results of these studies are pointing to an important tendency of PBM therapy to improve ddMSCs' viability and proliferation.
authors.affiliation: 1 Department of Restorative Dentistry, School of Dentistry, University of Sao Paulo , Sao Paulo, SP, Brazil .
authors.forename: Márcia Martins
authors.initials: MM
authors.lastname: Marques
authors.affiliation: 1 Department of Restorative Dentistry, School of Dentistry, University of Sao Paulo , Sao Paulo, SP, Brazil .
authors.forename: Ivana Márcia Alves
authors.initials: IM
authors.lastname: Diniz
authors.affiliation: 2 Department of Dentistry, School of Dentistry, Faculdades Metropolitanas Unidas , Sao Paulo, SP, Brazil .
authors.forename: Sueli Patricia Harumi Miyagi
authors.initials: SP
authors.lastname: de Cara
authors.affiliation: 1 Department of Restorative Dentistry, School of Dentistry, University of Sao Paulo , Sao Paulo, SP, Brazil .
authors.forename: Ana Clara Fagundes
authors.initials: AC
authors.lastname: Pedroni
authors.affiliation: 1 Department of Restorative Dentistry, School of Dentistry, University of Sao Paulo , Sao Paulo, SP, Brazil .
authors.forename: Gabriela Laranjeira
authors.initials: GL
authors.lastname: Abe
authors.affiliation: 3 School of Dentistry, Federal University of Para , Belem, PA, Brazil .
authors.forename: Roberta Souza
authors.initials: RS
authors.lastname: D'Almeida-Couto
authors.affiliation: 1 Department of Restorative Dentistry, School of Dentistry, University of Sao Paulo , Sao Paulo, SP, Brazil .
authors.forename: Paula Loures Valle
authors.initials: PL
authors.lastname: Lima
authors.affiliation: 4 Department of Biodentistry, School of Dentistry, Ibirapuera University , Sao Paulo, SP, Brazil .
authors.forename: Tamara Kerber
authors.initials: TK
authors.lastname: Tedesco
authors.affiliation: 4 Department of Biodentistry, School of Dentistry, Ibirapuera University , Sao Paulo, SP, Brazil .
authors.forename: Maria Stella
authors.initials: MS
authors.lastname: Moreira
mesh_terms: D049109:Cell Proliferation / Q000502:physiology / Q000528:radiation effects*; D003782:Dental Pulp / Q000166:cytology / Q000528:radiation effects*; D005260:Female; D006801:Humans; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D012038:Regeneration / Q000502:physiology / Q000528:radiation effects; D012680:Sensitivity and Specificity
keywords: dental pulp; low level laser; low power laser; photobiomodulation; phototherapy; stem cell