title: Tissue accumulation and urinary excretion of Cr in chromium picolinate (CrPic)-supplemented lambs.
abstract: Chromium (Cr) concentrations in liver, kidney, spleen, heart, lymph node, skeletal muscle, bone, testis and urine of lambs were measured to trace the biodistribution and bioaccumulation of Cr after oral supplementation with chromium picolinate (CrPic). Twenty-four Santa Inês lambs were treated with four different concentrations of CrPic: placebo, 0.250, 0.375 and 0.500 mg of CrPic/animal/day for 84 days. The basal diet consisted of Panicum maximum cv Massai hay and concentrate. Cr concentrations were measured by ICP-MS measuring (52)Cr as collected mass. There was a positive linear relationship between dose administered and the accumulation of Cr in the heart, lungs and testis. Urinary excretion of Cr occurred in a time and dose-dependent manner, so the longer or more dietary Cr provided, the greater excretion of the element. As some non-carcass components (such as lungs or heart) are added to bone and visceral meal to feed animals, there is a risk of bioaccumulation and biomagnification due to Cr offered as CrPic in the diet.
authors.affiliation: Faculdade de Agronomia e Medicina Veterinária, Universidade de Brasília-UnB, Campus Universitário Darcy Ribeiro, ICC-Sul, Brasília/DF, CEP 70910-900, Brazil; Instituto Nacional de Ciência e Tecnologia-Informação Genético-Sanitária da Pecuária Brasileira (CNPq/INCT/IGSPB), Belo Horizonte/MG, Brazil. Electronic address: dallago@unb.br.
authors.forename: Bruno Stéfano Lima
authors.initials: BS
authors.lastname: Dallago
authors.affiliation: Laboratório de Geocronologia, Universidade de Brasília-UnB, Rede Geochronos, Campus Universitário Darcy Ribeiro, Brasília/DF, CEP 70910-900, Brazil. Electronic address: barbara_alcantara@yahoo.com.br.
authors.forename: Bárbara Alcântara Ferreira
authors.initials: BA
authors.lastname: Lima
authors.affiliation: Laboratório de Microscopia Eletrônica, Instituto de Biologia, Universidade de Brasília-UnB, Campus Universitário Darcy Ribeiro, Brasília/DF, CEP 70910-900, Brazil. Electronic address: shelidabraz@gmail.com.
authors.forename: Shélida Vasconcelos
authors.initials: SV
authors.lastname: Braz
authors.affiliation: Laboratório de Patologia Veterinária, Universidade de Brasília-UnB, Campus Universitário Darcy Ribeiro, Hospital Veterinário L4-Norte, Brasília/DF, CEP 70910-900, Brazil. Electronic address: vanessamustafa@yahoo.com.br.
authors.forename: Vanessa da Silva
authors.initials: Vda S
authors.lastname: Mustafa
authors.affiliation: Faculdade de Agronomia e Medicina Veterinária, Universidade de Brasília-UnB, Campus Universitário Darcy Ribeiro, ICC-Sul, Brasília/DF, CEP 70910-900, Brazil; Instituto Nacional de Ciência e Tecnologia-Informação Genético-Sanitária da Pecuária Brasileira (CNPq/INCT/IGSPB), Belo Horizonte/MG, Brazil. Electronic address: concepta@unb.br.
authors.forename: Concepta
authors.initials: C
authors.lastname: McManus
authors.affiliation: Instituto Federal de Educação, Ciência e Tecnologia Goiano, Campus Iporá, Av. Oeste s/n, Iporá/GO, CEP 76200-000, Brazil. Electronic address: pradopaim@hotmail.com.
authors.forename: Tiago do Prado
authors.initials: Tdo P
authors.lastname: Paim
authors.affiliation: Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Av. Centenário, n° 303, Caixa Postal 96, Piracicaba/SP, CEP 13400-970, Brazil. Electronic address: nina.campeche@gmail.com.
authors.forename: Aline
authors.initials: A
authors.lastname: Campeche
authors.affiliation: Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Av. Centenário, n° 303, Caixa Postal 96, Piracicaba/SP, CEP 13400-970, Brazil. Electronic address: icedtoad@gmail.com.
authors.forename: Edgard Franco
authors.initials: EF
authors.lastname: Gomes
authors.affiliation: Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Av. Centenário, n° 303, Caixa Postal 96, Piracicaba/SP, CEP 13400-970, Brazil; Instituto Nacional de Ciência e Tecnologia-Informação Genético-Sanitária da Pecuária Brasileira (CNPq/INCT/IGSPB), Belo Horizonte/MG, Brazil. Electronic address: louvandini@cena.usp.br.
authors.forename: Helder
authors.initials: H
authors.lastname: Louvandini
mesh_terms: D000818:Animals; D002857:Chromium / Q000652:urine*; D019587:Dietary Supplements; D008297:Male; D009928:Organ Specificity / Q000187:drug effects; D010848:Picolinic Acids / Q000494:pharmacology*; D012044:Regression Analysis; D012756:Sheep; D014018:Tissue Distribution / Q000187:drug effects
chemical_list: D010848:Picolinic Acids; D002857:Chromium; C030614:picolinic acid
keywords: Bioaccumulation; Biodistribution; ICP–MS; Mineral supplement; Santa Inês