title: Stromal Vascular Fraction from Lipoaspirate Infranatant: Comparison Between Suction-Assisted Liposuction and Nutational Infrasonic Liposuction.
abstract: BACKGROUND
  Lipoaspirate has shown great promise as a source of progenitor cells for use in regenerative medicine. The stromal vascular fraction (SVF) can be isolated from lipoaspirate using enzyme digestion and centrifugation, but this approach may be limited by the labor-intensive nature of the technique as well as ambiguities in current governmental regulations. An alternative approach to obtain SVF from lipoaspirate was studied.
  
  
  METHODS
  Paired (collected from contralateral regions) lipoaspirate specimens were acquired from 30 consenting patients (age 24-62; 22 females, 8 males) by suction-assisted liposuction (SAL) and nutational infrasonic liposuction (NIL). The infranatant from 50 ml of adipose tissue (LAF) was centrifuged at 400g × 5 min and the resultant pellet was collected with a pipette. Time = 15-20 min. The respective SVFs cell populations were counted using an optical fluorescent cell counter (Nexcelom A2000) and the fluorescent stains-acridine orange (AO) and propidium iodide (PI).
  
  
  RESULTS
  The number of nucleated, live cells from SAL infranatant was 97,345 ± 23,435 per ml of adipose tissue and from NIL infranatant was 335,621 ± 81,274 per ml of adipose tissue. The p value is <0.00001, n = 30.
  
  
  CONCLUSIONS
  Regenerative cells can be isolated from the lipoaspirate infranatant from either SAL or NIL, although in lower quantities than from enzyme digestion. NIL acquisition yielded 3.5× the number of cells over that acquired from SAL. The time, skill, and cost of producing SVF from infranatant is less than using enzyme digestion, which potentially make these regenerative therapies accessible to more physicians and patients.
  
  
  METHODS
  This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
authors.affiliation: West Virginia University, 2000 Foundation Way #3700 Martinsburg, Morgantown, WV, 25401, USA. Rbowen3710@msn.com.
authors.forename: Robert E
authors.initials: RE
authors.lastname: Bowen
mesh_terms: D017667:Adipocytes / Q000637:transplantation*; D000273:Adipose Tissue / Q000601:surgery*; D000328:Adult; D005260:Female; D006801:Humans; D015187:Lipectomy / Q000379:methods*; D008297:Male; D008875:Middle Aged; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D017154:Stromal Cells / Q000637:transplantation; D013518:Surgery, Plastic / Q000379:methods; D020858:Tissue and Organ Harvesting / Q000379:methods; D014464:Ultrasonic Therapy / Q000379:methods*; D055815:Young Adult