title: Racemicystis crocea gen. nov., sp. nov., a soil myxobacterium in the family Polyangiaceae.
abstract: A novel bacterial strain designated MSr9521T was isolated in 2014 from a soil sample collected in 1986 from the Philippines. The novel bacterium shows myxobacterial characteristics that include pseudoplasmodial swarming, fruiting body formation and predatory ability to lyse microorganisms. The strain is chemoheterotrophic, mesophilic and aerobic. Major fatty acids are C18:1, C17:1 2-OH and iso-C15:0, and also contains trace amounts of omega-3/-6 polyunsaturated fatty acids. The G+C content of the genomic DNA is 70.4 mol%. The 16S rRNA gene sequence shows 95-96 % closest similarity to Sorangium cellulosum DSM 14627T, Polyangium fumosum Pl fu5T, Jahnella thaxteri Pl t4T and Byssovorax cruenta By c2T. The molecular phylogenetic analysis shows that the novel isolate forms a novel branch in the family Polyangiaceae, suborder Sorangiineae. Polyphasic taxonomic characterization suggests that the strain MSr9521T represents a novel species of a new genus in the family Polyangiaceae, for which the name Racemicystis crocea gen. nov., sp. nov. is proposed. The type strain of Racemicystis crocea is MSr9521T (=DSM 100773T=NCCB 100574T).
authors.affiliation: Department of Pharmaceutical Biotechnology, Saarland University, Building E8 1, 66123 Saarbrücken, Germany.|Department of Microbial Natural Products (MINS), Helmholtz Institute for Pharmaceutical Research Saarland (HIPS) - Helmholtz Centre for Infection Research (HZI), Saarland University Campus, Building E 8 1, 66123 Saarbrücken, Germany.
authors.forename: Ram Prasad
authors.initials: RP
authors.lastname: Awal
authors.affiliation: Department of Pharmaceutical Biotechnology, Saarland University, Building E8 1, 66123 Saarbrücken, Germany.|Department of Microbial Natural Products (MINS), Helmholtz Institute for Pharmaceutical Research Saarland (HIPS) - Helmholtz Centre for Infection Research (HZI), Saarland University Campus, Building E 8 1, 66123 Saarbrücken, Germany.|German Center for Infection Research (DZIF), Partner site Hannover-Braunschweig, 38124 Braunschweig, Germany.
authors.forename: Ronald
authors.initials: R
authors.lastname: Garcia
authors.affiliation: Department of Pharmaceutical Biotechnology, Saarland University, Building E8 1, 66123 Saarbrücken, Germany.|Department of Microbial Natural Products (MINS), Helmholtz Institute for Pharmaceutical Research Saarland (HIPS) - Helmholtz Centre for Infection Research (HZI), Saarland University Campus, Building E 8 1, 66123 Saarbrücken, Germany.|German Center for Infection Research (DZIF), Partner site Hannover-Braunschweig, 38124 Braunschweig, Germany.
authors.forename: Rolf
authors.initials: R
authors.lastname: Müller
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D009231:Myxococcales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010679:Philippines; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S