title: A new formula to calculate activity of superoxide dismutase in indirect assays.
abstract: To calculate superoxide dismutase (SOD) activity rapidly and accurately by indirect SOD assays, a formula based on the ratio of the catalytic speed of SOD to the reaction speed of the indicator with superoxide anion was deduced. The accuracy of this formula was compared with the conventional formula based on inhibition in five indirect SOD assays. The new formula was validated in nearly the entire SOD activity range, whereas the conventional formula was validated only during inhibition of 40-60%. This formula might also be used for the assays of other enzymes.
authors.affiliation: Institute of Biotechnology, Fuzhou University, Fuzhou, Fujian 350002, China; Biobased Chemistry & Technology, 6708 WG Wageningen, The Netherlands.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Wageningen UR Food & Biobased Research, 6708 WG Wageningen, The Netherlands.
authors.forename: Marieke E
authors.initials: ME
authors.lastname: Bruins
authors.affiliation: Institute of Biotechnology, Fuzhou University, Fuzhou, Fujian 350002, China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Yang
authors.affiliation: Institute of Biotechnology, Fuzhou University, Fuzhou, Fujian 350002, China. Electronic address: stliu@fzu.edu.cn.
authors.forename: Shu-Tao
authors.initials: ST
authors.lastname: Liu
authors.affiliation: CAS.SIBS-Zhejiang Gongshang University Joint Center for Food & Nutrition Research, Hangzhou 310035, China.
authors.forename: Ping-Fan
authors.initials: PF
authors.lastname: Rao
mesh_terms: D055162:Biocatalysis / Q000187:drug effects; D057075:Enzyme Assays / Q000379:methods*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019811:Hydroxylamine / Q000494:pharmacology; D007700:Kinetics; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013997:Time Factors
chemical_list: D004791:Enzyme Inhibitors; D019811:Hydroxylamine; D013482:Superoxide Dismutase
keywords: Cytochrome c; Epinephrine; Hydroxylamine hydrochloride; Nitroblue tetrazolium; SOD; WST