title: Deoxynivalenol inhibits proliferation and induces apoptosis in human umbilical vein endothelial cells.
abstract: Deoxynivalenol (DON) is a stable mycotoxins found in cereals infected by certain fungal species and causes adverse health effects in animals and human such as vomiting, diarrhea and reproductive toxicity. In this study, we investigated the toxic and apoptotic effects of DON in human umbilical vein endothelial cells (HUVECs), a good model for studying inflammation. The results show that DON significantly inhibited the viability of HUVECs. DON could also inhibit the proliferation of HUVECs through G2/M phase arrest in cell cycle progression. Moreover, oxidative stress induced by DON was indicated by observations of increased levels of reactive oxygen species (ROS). In addition, DON also causes mitochondrial damage by decreasing the mitochondrial membrane potential and inducing apoptosis by up-regulation of apoptosis-related genes like caspase-3, caspase-9, and Bax genes, and down-regulation of Bcl-2 gene. These results together suggest that DON could induce cell cycle arrest, oxidative stress, and apoptosis in HUVECs.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Deng
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Changyun
authors.initials: C
authors.lastname: Ji
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Weisen
authors.initials: W
authors.lastname: Qin
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Xifeng
authors.initials: X
authors.lastname: Cao
authors.affiliation: Center for Infection & Immunity, School of Basic Medical Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
authors.forename: Jialian
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Center for Infection & Immunity, School of Basic Medical Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
authors.forename: Yugu
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Biochemistry, University of Illinois, Urbana, IL 61801, USA.
authors.forename: Swaminath
authors.initials: S
authors.lastname: Srinivas
authors.affiliation: Center for Infection & Immunity, School of Basic Medical Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China. Electronic address: fengyj@zju.edu.cn.
authors.forename: Youjun
authors.initials: Y
authors.lastname: Feng
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. Electronic address: xbdeng@scau.edu.cn.
authors.forename: Xianbo
authors.initials: X
authors.lastname: Deng
mesh_terms: D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D015386:Hazardous Substances / Q000633:toxicity*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D014255:Trichothecenes / Q000633:toxicity*
chemical_list: D015386:Hazardous Substances; D017382:Reactive Oxygen Species; D014255:Trichothecenes; D053148:Caspase 3; D053453:Caspase 9; C007262:deoxynivalenol
keywords: Apoptosis; Cell cycle; Deoxynivalenol; HUVECs; Mitochondrial membrane potential