title: Antagonism of miRNA-21 Sensitizes Human Gastric Cancer Cells to Paclitaxel.
abstract: The development of drug resistance has largely limited the clinical outcome of anti-cancer treatment. Recent work has highlighted the involvement of non-coding RNAs, microRNAs (miRNAs), in cancer development. The present study aimed to investigate the role of miR-21 in the development of drug resistance to paclitaxel in gastric cancer cells. Our study found that the expression of miR-21 upregulated in the paclitaxel resistant cell line SGC7901/paclitaxel compared to its parental line SGC7901. Moreover, over-expression of miR-21 significantly decreased antiproliferative effects and apoptosis induced by paclitaxel, while knockdown of miR-21 dramatically increased antiproliferative effects and apoptosis induction by paclitaxel. Moreover, our results demonstrated that miR-21 may modulate the sensitivity to PTX, at least in part, by regulating the expression of P-glycoprotein.
authors.affiliation: Department of Gastroenteropathy Surgery, The Third Affiliated Hospital (Xinjiang Tumor Hospital), Xinjiang Medical University, Urumqi, 830011, Xinjiang, China. aupt1980@sina.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Jin
authors.affiliation: Department of Anesthesiology, The Third Affiliated Hospital (Xinjiang Tumor Hospital), Xinjiang Medical University, Urumqi, 830011, Xinjiang, China. qisanwang@sina.com.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Gastroenteropathy Surgery, The Third Affiliated Hospital (Xinjiang Tumor Hospital), Xinjiang Medical University, Urumqi, 830011, Xinjiang, China.
authors.forename: Haijiang
authors.initials: H
authors.lastname: Wang
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000378:metabolism; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004305:Dose-Response Relationship, Drug; D019008:Drug Resistance, Neoplasm; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors* / Q000235:genetics*; D017239:Paclitaxel / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D015854:Up-Regulation
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D000972:Antineoplastic Agents, Phytogenic; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D017239:Paclitaxel
keywords: Gastric cancer; P-glycoprotein; miRNA-21