title: Fabrication of porous gelatin-chitosan microcarriers and modeling of process parameters via the RSM method.
abstract: Porous gelatin-chitosan microcarriers (MCs) with the size of 350±50μm were fabricated with blends of different gelatin/chitosan (G/C) weight ratio using an electrospraying technique. Response surface methodology (RSM) was used to study the quantitative influence of process parameters, including blend ratio, voltage, and syringe pump flow rate, on MCs diameter and density. In the following, MCs of the same diameter and different G/C weight ratio (1, 2, and 3) were fabricated and their porosity and biocompatibility were investigated via SEM images and MTT assay, respectively. The results showed that mesenchymal stem cells (MSCs) could attach, proliferate, and spread on fabricated porous MCs during 7 days of culturing especially on those prepared with a G/C weight ratio of 1. Such porous gelatin-chitosan MCs with a G/C weight ratio of 1 may be considered as a promising candidate for injectable carriers supporting attachment and proliferation of MSCs.
authors.affiliation: Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Karimian S A
authors.affiliation: Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran. Electronic address: mashayekhan@sharif.edu.
authors.forename: Shohreh
authors.initials: S
authors.lastname: Mashayekhan
authors.affiliation: Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Baniasadi
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D004337:Drug Carriers; D055664:Electrochemical Techniques; D005163:Factor Analysis, Statistical; D005260:Female; D005780:Gelatin / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D016062:Porosity; D011247:Pregnancy; D061251:Primary Cell Culture; D054457:Tissue Scaffolds; D014470:Umbilical Cord / Q000166:cytology / Q000187:drug effects
chemical_list: D004337:Drug Carriers; D005780:Gelatin; D048271:Chitosan
keywords: Cell expansion; Gelatin/chitosan; Mesenchymal stem cells; Microcarriers; Response surface methodology