title: Neuron Culture from Mouse Superior Cervical Ganglion.
abstract: The rodent superior cervical ganglion (SCG) is a useful and readily accessible source of neurons for studying the mechanisms of sympathetic nervous system (SNS) development and growthin vitro.The sympathetic nervous system (SNS) of early postnatal animals undergoes a great deal of remodeling and development; thus, neurons taken from mice at this age are primed to re-grow and establish synaptic connections afterin situremoval. The stereotypic location and size of the SCG make it ideal for rapid isolation and dissociation. The protocol described here details the requirements for the dissection, culture and differentiation of SCG neurons. The protocol is suitable for culturing neurons from late embryonic gestation to approximately postnatal day 3. The culture technique discussed below utilizes glass coverslips for the microscopic examination of fixed cells.
authors.affiliation: Department of Neurology, Feinberg School of Medicine, Northwestern University, Chicago, USA.
authors.forename: Marisa
authors.initials: M
authors.lastname: Jackson
authors.affiliation: Department of Neurology, Feinberg School of Medicine, Northwestern University, Chicago, USA.
authors.forename: Warren
authors.initials: W
authors.lastname: Tourtellotte