title: Highly sensitive covalently functionalized light-addressable potentiometric sensor for determination of biomarker.
abstract: A biomarker is related to the biological status of a living organism and shows great promise for the early prediction of a related disease. Herein we presented a novel structured light-addressable potentiometric sensor (LAPS) for the determination of a model biomarker, human immunoglobulin G (hIgG). In this system, the goat anti-human immunoglobulin G antibody was used as recognition element and covalently immobilized on the surface of light-addressable potentiometric sensor chip to capture human immunoglobulin G. Due to the light addressable capability of light-addressable potentiometric sensor, human immunoglobulin G dissolved in the supporting electrolyte solution can be detected by monitoring the potential shifts of the sensor. In order to produce a stable photocurrent, the laser diode controlled by field-programmable gate array was used as the light emitter to drive the light-addressable potentiometric sensor. A linear correlation between the potential shift response and the concentration of human immunoglobulin G was achieved and the corresponding regression equation was ΔV (V)=0.00714ChIgG (μg/mL)-0.0147 with a correlation coefficient of 0.9968 over a range 0-150 μg/mL. Moreover, the light-addressable potentiometric sensor system also showed acceptable stability and reproducibility. All the results demonstrated that the system was more applicable to detection of disease biomarkers with simple operation, multiple-sample format and might hold great promise in various environmental, food, and clinical applications.
authors.affiliation: School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China; Guangxi Experiment Center of Information Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China.
authors.forename: Jintao
authors.initials: J
authors.lastname: Liang
authors.affiliation: School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China.
authors.forename: Mingyuan
authors.initials: M
authors.lastname: Guan
authors.affiliation: School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China.
authors.forename: Guoyin
authors.initials: G
authors.lastname: Huang
authors.affiliation: School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China.
authors.forename: Hengming
authors.initials: H
authors.lastname: Qiu
authors.affiliation: School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China.
authors.forename: Zhengcheng
authors.initials: Z
authors.lastname: Chen
authors.affiliation: School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China; Guangxi Experiment Center of Information Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China. Electronic address: liguiyin01@163.com.
authors.forename: Guiyin
authors.initials: G
authors.lastname: Li
authors.affiliation: School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China; Guangxi Experiment Center of Information Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China. Electronic address: huangyong503@126.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D015374:Biosensing Techniques; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D008027:Light; D057230:Limit of Detection; D056951:Photoelectron Spectroscopy; D011199:Potentiometry; D015203:Reproducibility of Results
chemical_list: D015415:Biomarkers; D007074:Immunoglobulin G
keywords: Biomarker; Human immunoglobulin G; Light-addressable potentiometric sensor