title: Molecular epidemiology of canine picornavirus in Hong Kong and Dubai and proposal of a novel genus in Picornaviridae.
abstract: Previously, we reported the discovery of a novel canine picornavirus (CanPV) in the fecal sample of a dog. In this molecular epidemiology study, CanPV was detected in 15 (1.11%) of 1347 canine fecal samples from Hong Kong and one (0.76%) of 131 canine fecal samples from Dubai, with viral loads 1.06×10(3) to 6.64×10(6) copies/ml. Complete genome sequencing and phylogenetic analysis showed that CanPV was clustered with feline picornavirus (FePV), bat picornavirus (BatPV) 1 to 3, Ia io picornavirus 1 (IaioPV1) and bovine picornavirus (BoPV), and this cluster was most closely related to the genera Enterovirus and Sapelovirus. The Ka/Ks ratios of all the coding regions were <0.1. According to the definition of the Picornavirus Study Group of ICTV, CanPV, FePV, BatPV 1 to 3, IaioPV1 and BoPV should constitute a novel genus in Picornaviridae. BEAST analysis showed that this genus diverged from its most closely related genus, Sapelovirus, about 49 years ago.
authors.affiliation: State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China; Department of Microbiology, The University of Hong Kong, Hong Kong, China; Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou 310006, China. Electronic address: pcywoo@hku.hk.
authors.forename: Patrick C Y
authors.initials: PCY
authors.lastname: Woo
authors.affiliation: State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China; Department of Microbiology, The University of Hong Kong, Hong Kong, China; Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou 310006, China.
authors.forename: Susanna K P
authors.initials: SKP
authors.lastname: Lau
authors.affiliation: Department of Microbiology, The University of Hong Kong, Hong Kong, China.
authors.forename: Garnet K Y
authors.initials: GKY
authors.lastname: Choi
authors.affiliation: Department of Microbiology, The University of Hong Kong, Hong Kong, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Central Veterinary Research Laboratory, Dubai, United Arab Emirates.
authors.forename: Saritha
authors.initials: S
authors.lastname: Sivakumar
authors.affiliation: Department of Microbiology, The University of Hong Kong, Hong Kong, China.
authors.forename: Hoi-Wah
authors.initials: HW
authors.lastname: Tsoi
authors.affiliation: Department of Microbiology, The University of Hong Kong, Hong Kong, China.
authors.forename: Cyril C Y
authors.initials: CCY
authors.lastname: Yip
authors.affiliation: Central Veterinary Research Laboratory, Dubai, United Arab Emirates.
authors.forename: Shanty V
authors.initials: SV
authors.lastname: Jose
authors.affiliation: Department of Microbiology, The University of Hong Kong, Hong Kong, China.
authors.forename: Ru
authors.initials: R
authors.lastname: Bai
authors.affiliation: Department of Microbiology, The University of Hong Kong, Hong Kong, China.
authors.forename: Emily Y M
authors.initials: EYM
authors.lastname: Wong
authors.affiliation: Central Veterinary Research Laboratory, Dubai, United Arab Emirates.
authors.forename: Marina
authors.initials: M
authors.lastname: Joseph
authors.affiliation: Department of Microbiology, The University of Hong Kong, Hong Kong, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Li
authors.affiliation: Central Veterinary Research Laboratory, Dubai, United Arab Emirates. Electronic address: cvrl@cvrl.ae.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Wernery
authors.affiliation: State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China; Department of Microbiology, The University of Hong Kong, Hong Kong, China; Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou 310006, China.
authors.forename: Kwok-Yung
authors.initials: KY
authors.lastname: Yuen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002415:Cats; D002417:Cattle; D002685:Chiroptera; D004283:Dog Diseases / Q000453:epidemiology* / Q000821:virology; D004285:Dogs; D005243:Feces / Q000821:virology; D016679:Genome, Viral; D006723:Hong Kong / Q000453:epidemiology; D017720:Molecular Epidemiology; D016366:Open Reading Frames; D010802:Phylogeny; D010849:Picornaviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010850:Picornaviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D012367:RNA, Viral / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014479:United Arab Emirates / Q000453:epidemiology; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins
keywords: animal RNA viruses; dog; picornavirus