title: [Prevalence of human papillomavirus infection in squamous cell carcinoma of the oral cavity, oropharynx and larynx].
abstract: BACKGROUND
  Cancer of the head and neck comprises a group of neoplasms that share a similar anatomical origin. Most originate from the epithelium of the aerodigestive tract and 90% correspond to squamous cell carcinoma. In the last 15 years, an increase in the incidence of squamous cell carcinoma induced by human papillomavirus (HPV) has been seen, mainly types 16 and 18, which are the most frequent found in cancers of the oral cavity and oropharynx, and types 6 and 11 in laryngeal cancer. There are reports in the literature that show HPV as the leading cause of oropharyngeal squamous cell carcinoma.
  
  
  OBJECTIVE
  Determine the prevalence of infection with high-risk HPV in patients diagnosed with squamous cell carcinoma of the oral cavity, oropharynx and larynx.
  
  
  METHODS
  An observational, cross-sectional, descriptive, unblinded study was performed. Prevalence of HPV infection was determined by polymerase chain reaction (PCR) in DNA samples from tumour tissue of patients with squamous cell carcinoma of the oral cavity, oropharynx and larynx. Typing was subsequently performed in HPV positive samples in order to detect types 18, 16, 11 and 6, using custom primers.
  
  
  RESULTS
  A total of 45 patients were included. The association between laryngeal squamous cell carcinoma and HPV was established in two patients, which represented an overall prevalence of 4.4% in our population, and 10% for laringeal tumours.
  
  
  CONCLUSIONS
  There is a low prevalence of HPV infection in squamous cell carcinoma of the oral cavity, oropharynx and larynx, in our population. Prospective studies on younger patients could provide more information.
authors.affiliation: Servicio de Otorrinolaringología, Hospital Universitario Dr. José Eleuterio González, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, México. Electronic address: centrodevoz@yahoo.com.mx.
authors.forename: Vicente José
authors.initials: VJ
authors.lastname: Villagómez-Ortíz
authors.affiliation: Servicio de Otorrinolaringología, Hospital Universitario Dr. José Eleuterio González, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, México.
authors.forename: Diana Estela
authors.initials: DE
authors.lastname: Paz-Delgadillo
authors.affiliation: Centro de Investigación y Desarrollo en Ciencias de la Salud, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, México.
authors.forename: Iván
authors.initials: I
authors.lastname: Marino-Martínez
authors.affiliation: Servicio de Anatomía Patológica, Hospital Universitario Dr. José Eleuterio González, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, México.
authors.forename: Luis Ángel
authors.initials: LÁ
authors.lastname: Ceseñas-Falcón
authors.affiliation: Servicio de Otorrinolaringología, Hospital Universitario Dr. José Eleuterio González, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, México.
authors.forename: Anabel
authors.initials: A
authors.lastname: Sandoval-de la Fuente
authors.affiliation: Servicio de Otorrinolaringología, Hospital Universitario Dr. José Eleuterio González, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, México.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Reyes-Escobedo
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology* / Q000821:virology; D003430:Cross-Sectional Studies; D015344:DNA Probes, HPV; D004273:DNA, Neoplasm / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006258:Head and Neck Neoplasms / Q000453:epidemiology* / Q000821:virology; D006801:Humans; D007822:Laryngeal Neoplasms / Q000453:epidemiology* / Q000821:virology; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000453:epidemiology* / Q000821:virology; D009959:Oropharyngeal Neoplasms / Q000453:epidemiology* / Q000821:virology; D027383:Papillomaviridae / Q000145:classification / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology; D015995:Prevalence; D012307:Risk Factors; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: D015344:DNA Probes, HPV; D004273:DNA, Neoplasm; D004279:DNA, Viral
keywords: Cabeza y cuello; Carcinoma espinocelular; Cavidad oral; Head and neck; Human papillomavirus; Laringe; Laryngeal; Oral cavity; Squamous cell carcinoma; Virus del papiloma humano