title: Biosensing made easy with PEG-targeted bi-specific antibodies.
abstract: Whilst recent advances in nanotechnology have yielded many new biosensing capabilities, innovative biological attachment and detection modalities remain relatively underdeveloped. Bi-specific antibodies (bsAbs)--which exhibit binding capability for two separate targets--offer an inherent advantage over conventional antibody reagents by significantly simplifying sensor surface preparation. Herein, we report the deployment of bsAbs for simultaneous attachment to a polymer-coated transducer and label-free, electrochemical (EC) detection of target antigens.
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology (AIBN), University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Lyndon J
authors.initials: LJ
authors.lastname: Raftery
authors.affiliation: Centre for Personalised NanoMedicine, AIBN, University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Yadveer S
authors.initials: YS
authors.lastname: Grewal
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology (AIBN), University of Queensland, Brisbane, QLD 4072, Australia and Centre for Advanced Imaging (CAI), University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Christopher B
authors.initials: CB
authors.lastname: Howard
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology (AIBN), University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Martina L
authors.initials: ML
authors.lastname: Jones
authors.affiliation: Centre for Personalised NanoMedicine, AIBN, University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Muhammad J A
authors.initials: MJ
authors.lastname: Shiddiky
authors.affiliation: Centre for Personalised NanoMedicine, AIBN, University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Laura G
authors.initials: LG
authors.lastname: Carrascosa
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology (AIBN), University of Queensland, Brisbane, QLD 4072, Australia and Centre for Advanced Imaging (CAI), University of Queensland, Brisbane, QLD 4072, Australia and ARC Centre of Excellence in Convergent BioNano Science and Technology, University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Kristofer J
authors.initials: KJ
authors.lastname: Thurecht
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology (AIBN), University of Queensland, Brisbane, QLD 4072, Australia and School of Chemical Engineering, University of Queensland, Brisbane, QLD 4072, Australia. s.mahler.eng.uq.edu.au.
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Mahler
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology (AIBN), University of Queensland, Brisbane, QLD 4072, Australia and Centre for Personalised NanoMedicine, AIBN, University of Queensland, Brisbane, QLD 4072, Australia and School of Chemistry and Molecular Biosciences (SCMB), University of Queensland, Brisbane, QLD 4072, Australia. m.trau@uq.edu.au.
authors.forename: Matt
authors.initials: M
authors.lastname: Trau
mesh_terms: D018033:Antibodies, Bispecific / Q000378:metabolism*; D015374:Biosensing Techniques; D011092:Polyethylene Glycols / Q000378:metabolism*
chemical_list: D018033:Antibodies, Bispecific; D011092:Polyethylene Glycols