title: Free and glycosylated simple phenol profiling in Apulian Italian wines.
abstract: Free simple phenols have a significant role in defining the sensory and nutritional characteristics of wines, affecting the organoleptic profile and having positive effects on health, but glycosidically bound phenols can also be hydrolysed during the winemaking process, releasing the corresponding volatile compounds and making a possible contribution to the final sensory profile. In this work, application of on-line SPE liquid chromatography-high resolution mass spectrometry, operating in negative polarity with heated electrospray, allowed to detect over eighty free and glycosylated simple phenols in Primitivo di Manduria and Negroamaro wines. Sixty-one phenols, four of which phenolic glucosidic precursors, were quantified as having quantification limits ranging from 0.001 to 0.1μgmL(-1), calibration R(2) of 0.99 for over 92% of compounds, and precision (R.S.D.%) always lower than 12%. Twenty-four simple phenolic precursors were tentatively identified as hexoside, pentoside and hexoside-hexoside derivatives, on the basis of accurate mass, isotopic pattern and MS/MS fragmentation.
authors.affiliation: Centro Trasferimento Tecnologico, Fondazione E. Mach, via E. Mach 1, 38010 San Michele all'Adige (TN), Italy.
authors.forename: C
authors.initials: C
authors.lastname: Barnaba
authors.affiliation: Universidad de la Republica Uruguay, Facultad de Quimica, Gral. Flores 2124, C.P. 11800 Montevideo, Uruguay.
authors.forename: E
authors.initials: E
authors.lastname: Dellacassa
authors.affiliation: Centro Trasferimento Tecnologico, Fondazione E. Mach, via E. Mach 1, 38010 San Michele all'Adige (TN), Italy.
authors.forename: G
authors.initials: G
authors.lastname: Nicolini
authors.affiliation: Centro Trasferimento Tecnologico, Fondazione E. Mach, via E. Mach 1, 38010 San Michele all'Adige (TN), Italy.
authors.forename: T
authors.initials: T
authors.lastname: Nardin
authors.affiliation: Centro Trasferimento Tecnologico, Fondazione E. Mach, via E. Mach 1, 38010 San Michele all'Adige (TN), Italy.
authors.forename: M
authors.initials: M
authors.lastname: Malacarne
authors.affiliation: Centro Trasferimento Tecnologico, Fondazione E. Mach, via E. Mach 1, 38010 San Michele all'Adige (TN), Italy. Electronic address: roberto.larcher@fmach.it.
authors.forename: R
authors.initials: R
authors.lastname: Larcher
mesh_terms: D002853:Chromatography, Liquid; D005504:Food Analysis; D006031:Glycosylation; D007558:Italy; D019800:Phenol / Q000032:analysis*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry; D014920:Wine / Q000032:analysis*
chemical_list: D019800:Phenol
keywords: Free simple phenols; Glycosylated simple phenols; Liquid chromatography; Negroamaro wines; On-line solid-phase extraction; Orbitrap; Primitivo di Manduria wines