title: Anti-Inflammatory Activity of Triterpenes Isolated from Protium paniculatum Oil-Resins.
abstract: Protium is the main genus of the Burseraceae family and one of the most common genera in South America, with an important species called "breu." Gum and oil-resins of this species are used as tonic and stimulant and for the treatment of ulcers and inflammation. The present study aims to isolate and investigate the anti-inflammatory activity of triterpene compounds isolated from oil-resin of Protium paniculatum. The pentacyclic triterpenes α,β-amyrin, acetylated α,β-amyrin, α,β-amyrone, and brein/maniladiol did not alter the viability of murine J774 macrophages (IC50 > 20 µg/mL), with the exception of mixture of brein/maniladiol which showed moderate cytotoxic activity. Also it was observed that compounds at 10 µg/mL inhibited more than 80% of production of NO(•), although only α,β-amyrin was able to inhibit the production of TNF-α (52.03 ± 2.4%). The compounds inhibited the production of IL-6 and induced the production of IL-10 in murine J774 macrophages stimulated by LPS. α,β-Amyrone inhibited the expression of COX-2 and also inhibited the formation of paw or ear edema in rats and mice, having a quick and immediate effect. This study may provide the basis for future investigations on the therapeutic role of α,β-amyrone in treating inflammation.
authors.affiliation: Laboratório de Atividade Biológica, Faculdade de Ciências Farmacêuticas, Universidade Federal do Amazonas (UFAM), Avenida Gen. Rodrigo Otavio, No. 6200, 69077-000 Manaus, AM, Brazil.
authors.forename: Patrícia D O
authors.initials: PD
authors.lastname: de Almeida
authors.affiliation: Laboratório de Atividade Biológica, Faculdade de Ciências Farmacêuticas, Universidade Federal do Amazonas (UFAM), Avenida Gen. Rodrigo Otavio, No. 6200, 69077-000 Manaus, AM, Brazil.
authors.forename: Ana Paula de A
authors.initials: AP
authors.lastname: Boleti
authors.affiliation: Instituto de Ciências Exatas, Departamento de Química, Universidade Federal do Amazonas, Avenida Gen. Rodrigo Otavio, No. 6200, 69077-000 Manaus, AM, Brazil.
authors.forename: André Luis
authors.initials: AL
authors.lastname: Rüdiger
authors.affiliation: Laboratório de Farmacologia, Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal do Amazonas, Avenida Gen. Rodrigo Otavio, No. 6200, 69077-000 Manaus, AM, Brazil.
authors.forename: Geane A
authors.initials: GA
authors.lastname: Lourenço
authors.affiliation: Instituto de Ciências Exatas, Departamento de Química, Universidade Federal do Amazonas, Avenida Gen. Rodrigo Otavio, No. 6200, 69077-000 Manaus, AM, Brazil.
authors.forename: Valdir Florêncio
authors.initials: VF
authors.lastname: da Veiga Junior
authors.affiliation: Laboratório de Atividade Biológica, Faculdade de Ciências Farmacêuticas, Universidade Federal do Amazonas (UFAM), Avenida Gen. Rodrigo Otavio, No. 6200, 69077-000 Manaus, AM, Brazil.
authors.forename: Emerson S
authors.initials: ES
authors.lastname: Lima