title: [RECONSTRUCTION OF LOWER EXTREMITY FUNCTION OF COMPLETE SPINAL CORD INJURY RATS BY FIRST NEURON CONNECTION].
abstract: OBJECTIVE
  To investigate the effects of the first neuron connection for the reconstruction of lower extremity function of complete spinal cord injury rats.
  
  
  METHODS
  Forty adult female Sprague Dawley rats of 300-350 g in weight were selected to prepare the models of L₁ transverse spinal cord injury. After 2 weeks of establishing model, the rats were randomly divided into control group (n = 20) and experimental group (n = 20). In the experimental group, the right hind limb function was reconstructed directly by the first neuron; in the control group, the other treatments were the same to the experimental group except that the distal tibial nerve and the proximal femoral nerve were not sutured. The recovery of motor function of lower extremity was observed by the Basso-Beattie-Bresnahan (BBB) scoring system on bilateral hind limbs at 7, 30, 50, and 70 days after operation. The changes of the spinal cord were observed by HE staining, neurofilament 200 immunohistochemistry staining, and the technique of horseradish peroxidase (HRP) tracing.
  
  
  RESULTS
  After establishing models, 6 rats died. The right hind limb had no obvious recovery of the motor function, with the BBB score of 0 in 2 groups; the left hind limb motor function was recovered in different degrees, and there was no significant difference in BBB score between 2 groups (P > 0.05). In the experimental group, HE staining showed that the spinal cord was reconstructed with the sciatic nerve, which was embedded in the spinal cord, and the sciatic nerve membrane was clearly identified, and there was no obvious atrophy in the connecting part of the spinal cord. In the experimental group, the expression of nerve fiber was stained with immunohistochemistry, and the axons of the spinal cord were positively by stained and the peripheral nerve was connected with the spinal cord. HRP labelled synapses were detected by HRP retrograde tracing in the experimental group, while there was no HRP labelled synapse in the control group.
  
  
  CONCLUSIONS
  Direct reconstruction of the first neurons is sufficient in the regeneration of corresponding neural circuit by the growth of residual axon; but the motor function recovery of the target muscles innervated by peripheral nerve is not observed.
authors.forename: Fangyong
authors.initials: F
authors.lastname: Wang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Jianjun
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D001369:Axons; D005260:Female; D006614:Hindlimb / Q000293:injuries / Q000503:physiopathology*; D007150:Immunohistochemistry; D035002:Lower Extremity / Q000503:physiopathology; D016900:Neurofilament Proteins / Q000378:metabolism*; D009474:Neurons; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D013119:Spinal Cord Injuries / Q000503:physiopathology* / Q000628:therapy
chemical_list: D016900:Neurofilament Proteins; C051774:neurofilament protein H