title: Candida glabrata Binding to Candida albicans Hyphae Enables Its Development in Oropharyngeal Candidiasis.
abstract: Pathogenic mechanisms of Candida glabrata in oral candidiasis, especially because of its inability to form hyphae, are understudied. Since both Candida albicans and C. glabrata are frequently co-isolated in oropharyngeal candidiasis (OPC), we examined their co-adhesion in vitro and observed adhesion of C. glabrata only to C. albicans hyphae microscopically. Mice were infected sublingually with C. albicans or C. glabrata individually, or with both species concurrently, to study their ability to cause OPC. Infection with C. glabrata alone resulted in negligible infection of tongues; however, colonization by C. glabrata was increased by co-infection or a pre-established infection with C. albicans. Furthermore, C. glabrata required C. albicans for colonization of tongues, since decreasing C. albicans burden with fluconazole also reduced C. glabrata. C. albicans hyphal wall adhesins Als1 and Als3 were important for in vitro adhesion of C. glabrata and to establish OPC. C. glabrata cell wall protein coding genes EPA8, EPA19, AWP2, AWP7, and CAGL0F00181 were implicated in mediating adhesion to C. albicans hyphae and remarkably, their expression was induced by incubation with germinated C. albicans. Thus, we found a near essential requirement for the presence of C. albicans for both initial colonization and establishment of OPC infection by C. glabrata.
authors.affiliation: Department of Oral Biology, School of Dental Medicine, University at Buffalo, Buffalo, New York, United States of America.
authors.forename: Swetha
authors.initials: S
authors.lastname: Tati
authors.affiliation: Department of Oral Biology, School of Dental Medicine, University at Buffalo, Buffalo, New York, United States of America.
authors.forename: Peter
authors.initials: P
authors.lastname: Davidow
authors.affiliation: Department of Oral Biology, School of Dental Medicine, University at Buffalo, Buffalo, New York, United States of America.
authors.forename: Andrew
authors.initials: A
authors.lastname: McCall
authors.affiliation: Department of Molecular Biology and Genetics, Johns Hopkins University, Baltimore, Maryland, United States of America.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Hwang-Wong
authors.affiliation: Department of Oral Biology, School of Dental Medicine, University at Buffalo, Buffalo, New York, United States of America.
authors.forename: Isolde G
authors.initials: IG
authors.lastname: Rojas
authors.affiliation: Department of Molecular Biology and Genetics, Johns Hopkins University, Baltimore, Maryland, United States of America.
authors.forename: Brendan
authors.initials: B
authors.lastname: Cormack
authors.affiliation: Department of Oral Biology, School of Dental Medicine, University at Buffalo, Buffalo, New York, United States of America.
authors.forename: Mira
authors.initials: M
authors.lastname: Edgerton
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000187:drug effects / Q000378:metabolism; D041221:Candida glabrata / Q000187:drug effects / Q000378:metabolism* / Q000472:pathogenicity; D002180:Candidiasis, Oral / Q000382:microbiology*; D002473:Cell Wall / Q000378:metabolism; D060085:Coinfection; D005260:Female; D015725:Fluconazole / Q000494:pharmacology*; D025301:Hyphae / Q000378:metabolism; D051379:Mice
chemical_list: D015725:Fluconazole