title: Aberrant expression of miR-127, miR-21 and miR-16 in placentas of deceased cloned sheep.
abstract: Placental deficiencies are associated with developmental abnormalities of animal produced by somatic cell nuclear transfer (SCNT). It is reported that aberrant expression of microRNAs (miRNAs) in the common placenta is associated with fetal growth restriction and placental deficiencies. However, an understanding of the expression and function of miRNAs in the placentas of cloned animal is lacking. In this study, we characterized the expression of five growth-associated miRNAs (miR-127, miR-16, miR-21, miR-93 and miR-182) in placentas of deceased transgenic cloned sheep (deceased group, n=7), live transgenic cloned sheep (live group, n=5) and conventionally produced sheep (control group, n=10). Expression levels of miR-127 (P<0.01), miR-21 (P<0.01) and miR-16 (P<0.05) were significantly up-regulated in the placentas of deceased group compared to that of control group. In contrast, the expression of these miRNAs was largely normal in the placentas of live group, except for the expression of miR-21. Furthermore, we confirmed that retrotransposon-like gene (Rtl1), a key gene in placental development, was down-regulated by miR-127 as a target in placenta cells. Our results suggested that the abnormal expression of miR-127, miR-21 and miR-16 in placentas of deceased sheep, through dysregulation of target genes, may result in developmental deficiencies of transgenic cloned sheep.
authors.affiliation: College of Life Sciences, Shihezi University, Shihezi, Xinjiang 832003, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ni
authors.affiliation: College of Life Sciences, Shihezi University, Shihezi, Xinjiang 832003, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: You
authors.affiliation: College of Life Sciences, Shihezi University, Shihezi, Xinjiang 832003, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Cao
authors.affiliation: College of Life Sciences, Shihezi University, Shihezi, Xinjiang 832003, China.
authors.forename: Cunyuan
authors.initials: C
authors.lastname: Li
authors.affiliation: College of Life Sciences, Shihezi University, Shihezi, Xinjiang 832003, China.
authors.forename: Junchuang
authors.initials: J
authors.lastname: Wei
authors.affiliation: College of Life Sciences, Shihezi University, Shihezi, Xinjiang 832003, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832003, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Qiao
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832003, China.
authors.forename: Xinxia
authors.initials: X
authors.lastname: Zhao
authors.affiliation: College of Life Sciences, Shihezi University, Shihezi, Xinjiang 832003, China. Electronic address: hushengwei@163.com.
authors.forename: Shengwei
authors.initials: S
authors.lastname: Hu
authors.affiliation: College of Life Sciences, Shihezi University, Shihezi, Xinjiang 832003, China. Electronic address: 32586999@qq.com.
authors.forename: Renzhe
authors.initials: R
authors.lastname: Quan
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D034561:Sheep, Domestic / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs
keywords: Placenta; SCNT; Transgenic cloned sheep; miR-127; miR-16; miR-21