title: In Vivo evaluation of adipogenic induction in fibrous and honeycomb-structured atelocollagen scaffolds.
abstract: Nowadays, soft tissue restoration techniques are mainly focused on volume regeneration instead of function recovering. So far, autologous fat transplant has been the most popular method although its multiple reported problems like volume and function loss. Adipose tissue engineering therefore emerges as a solution for development of biological substitutes for soft tissue which promotes not only volume regeneration but also function restoration with minimal consequences. Here we tested fibrous-structured atelocollagen (FSA) scaffolds and honeycomb atelocollagen (HCA) scaffolds for their ability to induce adipogenesis in vivo. Implants were subjected to histological and immunohistochemical assessment after 1, 2, and 4 weeks of implantation. Our studies showed that FSA scaffolds induced in vivo a markedly adipogenic response, whereas an acute inflammatory process was observed at HCA scaffolds without tissue regeneration detected within them. Our histological findings concerning FSA scaffolds clearly showed the presence of adipose-like tissue surprisingly composed by a mixture of brown-like and white-like adipocytes at week 2 whereas only white-like adipocytes at week 4. Subsequent positive Pax7 immunostaining at weeks 1 and 2 suggested the existence of a common myogenic progenitor shared by brown-like and white-like adipocytes observed. Then, in this work we present FSA scaffolds as a promising structure for brown and white adipose tissue engineering.
authors.affiliation: Department of Oral Pathology and Medicine, Graduated School of Medicine and Dentistry, Okayama University, 2-5- 1 Shikata-cho, Okayama 7008558, Japan; Laboratorio de Medios e Interfases, Departamento de Bioingeniería, Universidad Nacional de Tucumán, Consejo Nacional de Investigaciones Científicas y Técnicas, Av. Kirchner 1800, Tucumán, Argentina T4000.
authors.forename: Andrea P
authors.initials: AP
authors.lastname: Rodríguez
authors.affiliation: Laboratorio de Medios e Interfases, Departamento de Bioingeniería, Universidad Nacional de Tucumán, Consejo Nacional de Investigaciones Científicas y Técnicas, Av. Kirchner 1800, Tucumán, Argentina T4000. Electronic address: betiana_felice@yahoo.com.ar.
authors.forename: Betiana
authors.initials: B
authors.lastname: Felice
authors.affiliation: Laboratorio de Medios e Interfases, Departamento de Bioingeniería, Universidad Nacional de Tucumán, Consejo Nacional de Investigaciones Científicas y Técnicas, Av. Kirchner 1800, Tucumán, Argentina T4000.
authors.forename: María A
authors.initials: MA
authors.lastname: Sánchez
authors.affiliation: Department of Life Science, Okayama University of Science, 1-1 Ridai-cho, Kita-ku, Okayama 7000005, Japan.
authors.forename: Hidetsugu
authors.initials: H
authors.lastname: Tsujigiwa
authors.affiliation: Laboratorio de Medios e Interfases, Departamento de Bioingeniería, Universidad Nacional de Tucumán, Consejo Nacional de Investigaciones Científicas y Técnicas, Av. Kirchner 1800, Tucumán, Argentina T4000.
authors.forename: Carmelo J
authors.initials: CJ
authors.lastname: Felice
authors.affiliation: Department of Oral Pathology and Medicine, Graduated School of Medicine and Dentistry, Okayama University, 2-5- 1 Shikata-cho, Okayama 7008558, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nagatsuka
mesh_terms: D050156:Adipogenesis; D000273:Adipose Tissue / Q000378:metabolism / Q000473:pathology; D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D002454:Cell Differentiation; D003094:Collagen / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D016513:Mice, SCID; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D051763:PAX7 Transcription Factor / Q000378:metabolism; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D018952:Antigens, CD34; D051763:PAX7 Transcription Factor; C497322:Pax7 protein, mouse; C059821:atelocollagen; D003094:Collagen
keywords: Adipose tissue engineering; Atelocollagen; In vivo; Myogenic origin; White adipocytes