title: Simultaneous Determination of Aegeline and Six Coumarins from Different Parts of the Plant Aegle marmelos Using UHPLC-PDA-MS and Chiral Separation of Aegeline Enantiomers Using HPLC-ToF-MS.
abstract: A fast UHPLC-PDA method was developed for the simultaneous analysis of one alkaloid, aegeline, and six coumarins, viz., umbelliferone, scopoletin, marmesinin, 8-hydroxypsoralen, angelicin, and marmelosin, from the leaf, fruit, root, and bark of Aegle marmelos. The UHPLC method was validated for linearity, accuracy, repeatability, limits of detection and limits of quantification. The linearity range (r(2) > 0.99) of the seven compounds was found to be 0.5-250 µg/mL, and the limits of detection and limits of quantification for the seven compounds were found to be 0.1 and 0.5 µg/mL, respectively. The developed UHPLC method is simple, fast, and especially suitable for quality control analysis of coumarins and aegeline from A. marmelos and commercial dietary supplements. Single quadrupole mass spectrometry was used for the identification and confirmation of coumarins and aegeline from different plant parts and dietary supplements. In addition, a novel chiral HPLC-ToF-MS method was developed for the resolution of aegeline enantiomers. By applying this chiral method, the distribution of enantiomers of aegeline from different parts of A. marmelos and aegeline-containing dietary supplements is reported for the first time.
authors.affiliation: National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS, USA.
authors.forename: Bharathi
authors.initials: B
authors.lastname: Avula
authors.affiliation: National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS, USA.
authors.forename: Amar G
authors.initials: AG
authors.lastname: Chittiboyina
authors.affiliation: National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS, USA.
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Wang
authors.affiliation: National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS, USA.
authors.forename: Satyanarayanaraju
authors.initials: S
authors.lastname: Sagi
authors.affiliation: National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS, USA.
authors.forename: Vijayasankar
authors.initials: V
authors.lastname: Raman
authors.affiliation: National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS, USA.
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.affiliation: National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS, USA.
authors.forename: Ikhlas A
authors.initials: IA
authors.lastname: Khan
mesh_terms: D045471:Aegle / Q000737:chemistry*; D000577:Amides / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003374:Coumarins / Q000032:analysis*; D019587:Dietary Supplements / Q000032:analysis; D005638:Fruit / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013237:Stereoisomerism
chemical_list: D000577:Amides; D003374:Coumarins; C560978:aegeline