title: Establishment and Characterization of Naïve Pluripotency in Human Embryonic Stem Cells.
abstract: Mouse embryonic stem cells are known to represent the naïve state of pluripotency, while human embryonic stem cells typically represented the primed state of pluripotency, characterized by a higher drift toward differentiation and some other disadvantages. Here we describe an efficient method for rapid, transgene free induction of the naïve pluripotent state in human by applying a novel combination of small molecules and growth factors in the culture medium (2i, LIF, basic fibroblast growth factor, ascorbic acid, and forskolin). Conversion of primed human embryonic stem cells towards the naive pluripotent state should be confirmed by a detailed characterization of the cells, as described in this chapter.
authors.affiliation: Ghent-Fertility and Stem cell Team (G-FaST), Department for Reproductive Medicine, Ghent University Hospital, De Pintelaan 185, 9000, Ghent, Belgium.
authors.forename: Sharat
authors.initials: S
authors.lastname: Warrier
authors.affiliation: Ghent-Fertility and Stem cell Team (G-FaST), Department for Reproductive Medicine, Ghent University Hospital, De Pintelaan 185, 9000, Ghent, Belgium.
authors.forename: Mina
authors.initials: M
authors.lastname: Popovic
authors.affiliation: Ghent-Fertility and Stem cell Team (G-FaST), Department for Reproductive Medicine, Ghent University Hospital, De Pintelaan 185, 9000, Ghent, Belgium.
authors.forename: Margot
authors.initials: M
authors.lastname: Van der Jeught
authors.affiliation: Ghent-Fertility and Stem cell Team (G-FaST), Department for Reproductive Medicine, Ghent University Hospital, De Pintelaan 185, 9000, Ghent, Belgium. bjorn.heindryckx@ugent.be.
authors.forename: Björn
authors.initials: B
authors.lastname: Heindryckx
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D000066449:Human Embryonic Stem Cells / Q000187:drug effects*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D051379:Mice; D000066450:Mouse Embryonic Stem Cells / Q000187:drug effects*; D015398:Signal Transduction / Q000187:drug effects; D054852:Small Molecule Libraries / Q000494:pharmacology
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D054852:Small Molecule Libraries
keywords: Human embryonic stem cells; Immunostaining; Karyotyping; Mouse embryonic fibroblasts; Naïve pluripotency; Quantitative real-time PCR