title: Effect of autologous platelet-rich plasma application on cutaneous wound healing in dogs.
abstract: This study was conducted to identify the effectiveness of platelet-rich plasma (PRP) and efficacy of intralesional injection as a method of application to acute cutaneous wounds in dogs. Healthy adult beagles (n = 3) were used in this study. Autologous PRP was separated from anticoagulant treated whole blood in three dogs. Cutaneous wounds were created and then treated by intralesional injection of PRP in the experimental group, while they were treated with saline in the control group on days 0, 2 and 4. The healing process was evaluated by gross examination throughout the experimental period and histologic examination on day 7, 14 and 21. In PRP treated wounds, the mean diameter was smaller and the wound closure rate was higher than in the control. Histological study revealed that PRP treated wounds showed more granulation formation and angiogenesis on day 7, and faster epithelialization, more granulation formation and collagen deposition were observed on day 14 than in control wounds. On day 21, collagen deposition and epithelialization were enhanced in PRP treated groups. Overall, PRP application showed beneficial effects in wound healing, and intralesional injection was useful for application of PRP and could be a good therapeutic option for wound management in dogs.
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Cho-Hee
authors.initials: CH
authors.lastname: Jee
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Na-Young
authors.initials: NY
authors.lastname: Eom
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Hyo-Mi
authors.initials: HM
authors.lastname: Jang
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Jung
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Eul-Soo
authors.initials: ES
authors.lastname: Choi
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Jin-Hee
authors.initials: JH
authors.lastname: Won
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Il-Hwa
authors.initials: IH
authors.lastname: Hong
authors.affiliation: Laboratory of Veterinary Dermatology and Neurology, College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Korea.
authors.forename: Byeong-Teck
authors.initials: BT
authors.lastname: Kang
authors.affiliation: Family Medicine Clinic and Research Institute of Convergence of Biomedical Sciences and Technology, Pusan National University Yangsan Hospital, Pusan National University School of Medicine, Yangsan 50612, Korea.
authors.forename: Dong Wook
authors.initials: DW
authors.lastname: Jeong
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Dong-In
authors.initials: DI
authors.lastname: Jung
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism; D020405:Dermis / Q000166:cytology / Q000293:injuries / Q000502:physiology; D004285:Dogs; D000078404:Epidermal Cells; D004817:Epidermis / Q000293:injuries / Q000502:physiology*; D005260:Female; D006097:Granulation Tissue / Q000166:cytology; D015552:Injections, Intralesional / Q000662:veterinary; D008297:Male; D018919:Neovascularization, Physiologic; D053657:Platelet-Rich Plasma; D012038:Regeneration; D016896:Treatment Outcome; D014945:Wound Healing; D014947:Wounds and Injuries / Q000628:therapy / Q000662:veterinary*
chemical_list: D003094:Collagen
keywords: cutaneous wound; dog; intralesional injection; platelet-rich plasma; wound healing