title: The C609T (Pro187Ser) Null Polymorphism of the NQO1 Gene Contributes Significantly to Breast Cancer Susceptibility in North Indian Populations: a Case Control Study.
abstract: BACKGROUND
  Worldwide, breast cancer is the most common cancer among women and is a leading cause of cancer death. In the present study, we investigated the NQO1 C609T genotypic and allelic distribution in north Indian breast cancer patients.
  
  
  METHODS
  The genotypic distribution of the NQ01 C609T polymorphism was assessed in 100 invasive ductal carcinoma (IDC) breast cancer patients and 100 healthy controls using allele specific PCR (AS-PCR).
  
  
  RESULTS
  A lower frequency of the CC genotype was found in breast cancer patients (24%) than in the controls. On the other hand, TT genotype frequency was also found to be higher in female healthy controls (32%) than the female breast cancer patients (20%). The frequencies of all three genotypes CC, CT, TT in patients were 24%, 56% and 20% and in healthy controls 50%, 22% and 32% respectively. We did not find any significant correlation between the NQO1 C609T polymorphism and age group, grading, menopausal status and distant metastasis. A less significant association was found between the NQ01 C609T polymorphism and the stage of breast cancer (X2=5.931, P=0.05).
  
  
  CONCLUSIONS
  The present study shows a strong association between NQO1 C609T polymorphism with the breast cancer risk in the north Indian breast cancer patients so that possible use as a risk factor should be further explored.
authors.affiliation: Department of Biochemistry, Maulana Azad Medical College and Associated Hospitals, New Delhi, India E-mail : prasant.mamc@gmail.com.
authors.forename: Prasant
authors.initials: P
authors.lastname: Yadav
authors.forename: Rashid
authors.initials: R
authors.lastname: Mir
authors.forename: Kajal
authors.initials: K
authors.lastname: Nandi
authors.forename: Jamsheed
authors.initials: J
authors.lastname: Javid
authors.forename: Mirza
authors.initials: M
authors.lastname: Masroor
authors.forename: Imtiyaz
authors.initials: I
authors.lastname: Ahmad
authors.forename: Mariyam
authors.initials: M
authors.lastname: Zuberi
authors.forename: Rcm
authors.initials: R
authors.lastname: Kaza
authors.forename: Sk
authors.initials: S
authors.lastname: Jain
authors.forename: Nita
authors.initials: N
authors.lastname: Khurana
authors.forename: Prakash Chandra
authors.initials: PC
authors.lastname: Ray
authors.forename: Alpana
authors.initials: A
authors.lastname: Saxena
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D001943:Breast Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D005500:Follow-Up Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007194:India / Q000453:epidemiology; D008875:Middle Aged; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics*; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D012307:Risk Factors
chemical_list: D014408:Biomarkers, Tumor; D016660:NAD(P)H Dehydrogenase (Quinone); C447479:NQO1 protein, human