title: Novel leptin OB3 peptide-induced signaling and progression in thyroid cancers: Comparison with leptin.
abstract: Obesity results in increased secretion of cytokines from adipose tissue and is a risk factor for various cancers. Leptin is largely produced by adipose tissue and cancer cells. It induces cell proliferation and may serve to induce various cancers. OB3-leptin peptide (OB3) is a new class of functional leptin peptide. However, its mitogenic effect has not been determined. In the present study, because of a close link between leptin and the hypothalamic-pituitary-thyroid axis, OB3 was compared with leptin in different thyroid cancer cells for gene expression, proliferation and invasion. Neither agent stimulated cell proliferation. Leptin stimulated cell invasion, but reduced adhesion in anaplastic thyroid cancer cells. Activated ERK1/2 and STAT3 contributed to leptin-induced invasion. In contrast, OB3 did not affect expression of genes involved in proliferation and invasion. In vivo studies in the mouse showed that leptin, but not OB3, significantly increased circulating levels of thyrotropin (TSH), a growth factor for thyroid cancer. In summary, OB3 is a derivative of leptin that importantly lacks the mitogenic effects of leptin on thyroid cancer cells.
authors.affiliation: Joint Biobank, Office of Human Research, Taipei Medical University, Taipei, Taiwan.
authors.forename: Yu-Chen Sh
authors.initials: YC
authors.lastname: Yang
authors.affiliation: Taipei Cancer Center, Taipei Medical University, Taipei, Taiwan.
authors.forename: Yu-Tang
authors.initials: YT
authors.lastname: Chin
authors.affiliation: Taipei Cancer Center, Taipei Medical University, Taipei, Taiwan.|PhD Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan.
authors.forename: Meng-Ti
authors.initials: MT
authors.lastname: Hsieh
authors.affiliation: Taipei Cancer Center, Taipei Medical University, Taipei, Taiwan.
authors.forename: Hsuan-Yu
authors.initials: HY
authors.lastname: Lai
authors.affiliation: Biomedical Imaging Research Center, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Chien-Chih
authors.initials: CC
authors.lastname: Ke
authors.affiliation: Center for Immunology and Microbial Disease, Albany Medical College, Albany, New York, USA.
authors.forename: Dana R
authors.initials: DR
authors.lastname: Crawford
authors.affiliation: Stem Cell Research Center, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Oscar K
authors.initials: OK
authors.lastname: Lee
authors.affiliation: Department of Periodontology, School of Dentistry, National Defense Medical College, Taipei, Taiwan.
authors.forename: Earl
authors.initials: E
authors.lastname: Fu
authors.affiliation: Pharmaceutical Research Institute, Albany College of Pharmacy and Health Sciences, Albany, New York, USA.
authors.forename: Shaker A
authors.initials: SA
authors.lastname: Mousa
authors.affiliation: Department of Medicine, Albany Medical College, Albany, New York, USA.
authors.forename: Patricia
authors.initials: P
authors.lastname: Grasso
authors.affiliation: Taipei Cancer Center, Taipei Medical University, Taipei, Taiwan.
authors.forename: Leroy F
authors.initials: LF
authors.lastname: Liu
authors.affiliation: Department of Biochemistry and Molecular Cell Biology, College of Medicine, Taipei Medical University, Taipei, Taiwan.|Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.
authors.forename: Heng-Yu
authors.initials: HY
authors.lastname: Chang
authors.affiliation: Pharmaceutical Research Institute, Albany College of Pharmacy and Health Sciences, Albany, New York, USA.
authors.forename: Heng-Yuan
authors.initials: HY
authors.lastname: Tang
authors.affiliation: Taipei Cancer Center, Taipei Medical University, Taipei, Taiwan.|PhD Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan.
authors.forename: Hung-Yun
authors.initials: HY
authors.lastname: Lin
authors.affiliation: Pharmaceutical Research Institute, Albany College of Pharmacy and Health Sciences, Albany, New York, USA.|Department of Medicine, Albany Medical College, Albany, New York, USA.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Davis
mesh_terms: D000818:Animals; D050260:Carbohydrate Metabolism / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D018450:Disease Progression; D006801:Humans; D020738:Leptin / Q000378:metabolism / Q000493:pharmacokinetics / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010446:Peptide Fragments / Q000378:metabolism / Q000494:pharmacology*; D011897:Random Allocation; D015398:Signal Transduction; D013964:Thyroid Neoplasms / Q000097:blood / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D013972:Thyrotropin / Q000097:blood
chemical_list: D020738:Leptin; D010446:Peptide Fragments; C410793:leptin (116-130); D013972:Thyrotropin
keywords: OB3-leptin peptide; cancer cell invasion; leptin; obesity