title: [EVALUATION OF THE BACTERIOSTATIC EFFECT OF IMMOBILIZED ON COLLAGEN CARRIER ANTIBIOTICS AND SILVER IONS IN PROVISION OF THE ASEPTIC STATE OF THE TISSUE WOUNDS].
abstract: In the treatment of tissue wounds practically all the time there is necessary the establishment of aseptic conditions in the area of the damaged tissue. In modern medical practice quite a while the use of materials on the basis of collagen aroused widespread. The aim of the study was an assessment the bacteriostatic properties both of collagenic substance with the antibiotics immobilized on it, and ions of silver also. The presence on a surface of collagen of a large number of active functional groups allow to use it as a matrix for an immobilization of various biologically active and medicinal agents. Authors performed an assessment of possibility of an immobilization of antibiotics on the collagenic carrier. The purpose of an immobilization was in the prolongation of the impact of an active component--Polymyxin-M or Gentamycin. Besides aseptic effect, availability of specific amino acids of collagen will promote the acceleration of the reparative processes in a wound. There were performed investigations on determination of bacteriostatic effect of immobilized on the protein carrier (collagen of hydrobionts) antibiotics, and silver ions also. Bacteriostatic effect was estimated by the disco--a diffusive method with use of E. coli as test culture. Results of the studies showed that the delivered preparation had bacteriostatic effect on the test culture. At that the area of a zone of a growth inhibition in case of the of use--Polymyxin was 1.3 time more than, than in case of the use of a preparation of Gentamycin. The obtained collagenic substances of an origin with the immobilized bacteriostatic components and ions of silver have considerable prospects and real opportunity in the delivery of aseptic preparations of a domestic production. Taking into account the available data in further there is planned the development of aseptic preparations with the occluded biologically active agents and nanoparticles of metals for providing an aseptics and acceleration the reparative processes in the treatment of open wounds.
authors.forename: S A
authors.initials: SA
authors.lastname: Storublevtsev
authors.forename: V I
authors.initials: VI
authors.lastname: Popov
authors.forename: L V
authors.initials: LV
authors.lastname: Antipova
authors.forename: O G
authors.initials: OG
authors.lastname: Stukalo
authors.forename: S B
authors.initials: SB
authors.lastname: Bolgova
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003094:Collagen; D004337:Drug Carriers; D004926:Escherichia coli / Q000187:drug effects* / Q000302:isolation & purification; D006801:Humans; D012834:Silver; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D012834:Silver; D003094:Collagen