title: [Dental plaque microcosm biofilm behavior on a resin composite incorporated with nano-antibacterial inorganic filler containing long-chain alkyl quaternary ammonium salt].
abstract: OBJECTIVE
  To develop a resin composite incorporated with nano-antibacterial inorganic filler containing long-chain alkyl quaternary ammonium salt, and to measure its effect on human dental plaque microcosm biofilm.
  
  
  METHODS
  A novel nano-antibacterial inorganic filler containing long-chain alkyl quaternary ammonium salt was synthesized according to methods introduced in previous research. Samples of the novel nano-antibacterial inorganic fillers were modified by a coupling agent and then added into resin composite at 0%, 5%, 10%, 15% or 20% mass fractions; 0% composite was used as control. A flexural test was used to measure resin composite mechanical properties. Results showed that a dental plaque microcosm biofilm model with human saliva as inoculum was formed. Colony-forming unit (CFU) counts, lactic acid production, and live/dead assay of biofilm on the resin composite were calculated to test the effect of the resin composite on human dental plaque microcosm biofilm.
  
  
  RESULTS
  The incorporation of nano-antibacterial inorganic fillers with as much as 15% concentration into the resin composite showed no adverse effect on the mechanical properties of the resin composite (P > 0.05). Resin composite containing 5% or more nano-antibacterial inorganic fillers significantly inhibited the metabolic activity of dental plaque microcosm biofilm, suggesting its strong antibacterial potency (P < 0.05).
  
  
  CONCLUSIONS
  This novel resin composite exhibited a strong antibacterial property upon the addition of up to 5% nano-antibacterial inorganic fillers, thereby leading to effective caries inhibition in dental application.
authors.forename: Wu
authors.initials: W
authors.lastname: Junling
authors.forename: Zhang
authors.initials: Z
authors.lastname: Qiang
authors.forename: Sun
authors.initials: S
authors.lastname: Ruinan
authors.forename: Zhu
authors.initials: Z
authors.lastname: Ting
authors.forename: Ge
authors.initials: G
authors.lastname: Jianhua
authors.forename: Zhou
authors.initials: Z
authors.lastname: Chuanjian
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects*; D003188:Composite Resins / Q000737:chemistry*; D003731:Dental Caries / Q000517:prevention & control; D003773:Dental Plaque; D006801:Humans; D019344:Lactic Acid; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D012463:Saliva
chemical_list: D000900:Anti-Bacterial Agents; D003188:Composite Resins; D000644:Quaternary Ammonium Compounds; D019344:Lactic Acid