title: Dissecting the Role of K61/K59 Residue in VPS4 Functions.
abstract: ESCRTs (Endosomal Sorting Complexes Required for Transport) are required for the formation of the intraluminal vesicles in the multivesicular bodies and are involved in other topologically similar processes such as cytokinesis, nuclear envelope sealing and viral egress. The final complex ESCRT-III is disassembled by the recruitment and activation of the AAA-ATPase VPS4 to the endosomal membranes. This recruitment is due to the binding of VPS4 N-terminal MIT with MIM1 and MIM2 domains present in the CHMPs proteins. By analyzing different cellular membrane remodeling events in which VPS4 is involved, here we provide evidence that the K61 residue, mapping within the MIT domain of VPS4B (K59 in VPS4A), is involved in VPS4 functioning. Posttranslational modifications of this residue might modulate MIT-MIM2 binding affinity and, as a consequence, VPS4 functions.
authors.forename: Luca
authors.initials: L
authors.lastname: Cesaro
authors.forename: Marta
authors.initials: M
authors.lastname: Toffoletto
authors.forename: Arianna
authors.initials: A
authors.lastname: Calistri
authors.affiliation: Department of Biomedical Sciences, University of Padova, Via Ugo Bassi 58/B, Padova, Italy. mauro.salvi@unipd.it.
authors.forename: Mauro
authors.initials: M
authors.lastname: Salvi
mesh_terms: D000074183:ATPases Associated with Diverse Cellular Activities; D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism*; D056827:Endosomal Sorting Complexes Required for Transport / Q000378:metabolism*; D011992:Endosomes / Q000378:metabolism*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D025262:Vacuolar Proton-Translocating ATPases / Q000378:metabolism*
chemical_list: D056827:Endosomal Sorting Complexes Required for Transport; D025262:Vacuolar Proton-Translocating ATPases; D000074183:ATPases Associated with Diverse Cellular Activities; C490305:VPS4A protein, human