title: Role of TWIST2, E-cadherin and Vimentin in epithelial ovarian carcinogenesis and prognosis and their interaction in cancer progression.
abstract: Globally, most patients are at late-stage when they have been diagnosed with ovarian cancer. Investigating the potential mechanisms involved in tumor progression and prognosis is essential for improving treatment options, outcomes, and survival.
  
  
  OBJECTIVE
  This study elucidated the clinico-pathological significance of TWIST2 and the relationship of TWIST2, E-cadherin, and Vimentin expression in the progression and prognosis of epithelial ovarian cancer (EOC).
  
  
  METHODS
  Immunohistochemical staining was used to quantify the expression and relevance of TWIST2, E-cadherin, and Vimentin in 103 ovarian specimens, including 30 cases of benign ovarian tumors, 30 cases of borderline ovarian tumors, and 43 cases of EOC.
  
  
  RESULTS
  The expression of TWIST2 in the cytoplasm may help to maintain characteristics of epithelial cancer cells with E-cadherin normal membranous expression, while nuclear TWIST2 induces tumor translation front with membranous expression of Vimentin, which eventually promotes cancer metastasis. Moreover, the upregulation of TWIST2 was also related to the aberrant expression of E-cadherin and the increased expression of Vimentin, which were reported as important indicators of epithelial-mesenchymal transition (EMT).
  
  
  CONCLUSIONS
  The data suggested that co-expression of TWIST2/Vimentin was an independent prognostic indicator for both overall survival and disease-free survival by multivariate Cox proportional hazards model. TWIST2 regulates EMT by depriving the epithelial cell phenotype of E-cadherin and endowing the mesenchymal cell phenotype with Vimentin, which may be involved in the progression and prognosis of ovarian cancer, and TWIST2/Vimentin co-expression might be a novel indicator with prognostic potential in EOC patients.
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Liang
authors.forename: H
authors.initials: H
authors.lastname: Xing
mesh_terms: D015820:Cadherins / Q000032:analysis / Q000502:physiology*; D000077216:Carcinoma, Ovarian Epithelial; D018450:Disease Progression; D005260:Female; D006801:Humans; D009375:Neoplasms, Glandular and Epithelial / Q000209:etiology*; D010051:Ovarian Neoplasms / Q000209:etiology*; D011379:Prognosis; D016016:Proportional Hazards Models; D012097:Repressor Proteins / Q000032:analysis / Q000502:physiology*; D051879:Twist-Related Protein 1 / Q000032:analysis / Q000502:physiology*; D014746:Vimentin / Q000032:analysis / Q000502:physiology*
chemical_list: D015820:Cadherins; D012097:Repressor Proteins; C486274:TWIST2 protein, human; D051879:Twist-Related Protein 1; D014746:Vimentin