title: Circulating LncRNAs as Novel, Non-Invasive Biomarkers for Prenatal Detection of Fetal Congenital Heart Defects.
abstract: OBJECTIVE
  To explore the clinical value of circulating long non-coding RNAs (lncRNAs) as biomarkers to predict fetal congenital heart defects (CHD) in pregnant women.
  
  
  METHODS
  Differential expression of lncRNAs isolated from the plasma of pregnant women with typical fetal CHD or healthy controls was analyzed by microarray. Gene ontology (GO), pathway and network analysis were performed to study the function of the lncRNAs. Differentially expressed lncRNAs were validated in plasma samples from 62 pregnant women with typical CHD and 62 matched controls by RT-PCR. The sensitivity and specificity of each lncRNA in the diagnosis of fetal CHD was determined by ROC curve analysis.
  
  
  RESULTS
  Microarray analysis identified 3694 up-regulated and 3919 down-regulated (fold change ≥2.0) lncRNAs. The top ten significantly differentially expressed, CHD-associated lncRNAs were validated by RT-PCR. Five significantly up-regulated or down-regulated lncRNAs were identified: ENST00000436681, ENST00000422826, AA584040, AA709223 and BX478947 with the AUC of ROC curves calculated as 0.892, 0.817, 0.755, 0.882 and 0.886, respectively.
  
  
  CONCLUSIONS
  Specific lncRNAs aberrantly expressed in the plasma of pregnant women with typical fetal CHD may play a key role in the development of CHD and may be used as novel biomarkers for prenatal diagnosis of fetal CHD.
authors.forename: Meng
authors.initials: M
authors.lastname: Gu
authors.forename: Aibin
authors.initials: A
authors.lastname: Zheng
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Tu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Li
authors.forename: Shuping
authors.initials: S
authors.lastname: Han
authors.forename: Xiaoshan
authors.initials: X
authors.lastname: Hu
authors.forename: Jingai
authors.initials: J
authors.lastname: Zhu
authors.forename: Ya
authors.initials: Y
authors.lastname: Pan
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.forename: Zhangbin
authors.initials: Z
authors.lastname: Yu
mesh_terms: D000328:Adult; D019540:Area Under Curve; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D015536:Down-Regulation; D005260:Female; D006330:Heart Defects, Congenital / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D011247:Pregnancy; D011296:Prenatal Diagnosis; D062085:RNA, Long Noncoding / Q000097:blood*; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; D062085:RNA, Long Noncoding