title: NOVEL PROTEINS COMPRISING THE STRAMENOPILE TRIPARTITE MASTIGONEME IN OCHROMONAS DANICA (CHRYSOPHYCEAE)(1).
abstract: Two-dimensional (2-D) protein analysis of the mastigoneme fraction of the chromophyte alga Ochromonas danica E. G. Pringsh. showed the presence of several component proteins of the tubular mastigoneme. Adding to the reported gene Ocm1, three new genes (Ocm2, Ocm3, and Ocm4) belonging to the Ocm gene family were isolated using degenerate primers designed from predicted Ocm1 amino acid sequences. The predicted polypeptides encoded by Ocm2, Ocm3, and Ocm4 were smaller in size than Ocm1. However, they shared four highly conserved, cysteine-rich, epithelial growth factor (EGF)-like motifs, potentially involved in protein-protein interaction. In addition, Ocm2, Ocm3, and Ocm4 showed homology to the SIG protein family in the centric diatom Thalassiosira weissflogii (Grunow) Fryxell et Hasle, which is up-regulated during early stages of sexual reproduction. Immunofluorescence analysis with a polyclonal antibody against the partial amino acid sequences of Ocm2, Ocm3, and Ocm4 showed that Ocm2 and Ocm3 were located in the basal segment region of mastigonemes attached on the surface of the anterior flagellum, and that Ocm4 was located within the tubular shaft portion similar to Ocm1.
authors.affiliation: Research Center for Inland Seas, Kobe University, Kobe 657-8501, JapanMuroran Marine Station, Field Science Center for Northern Biosphere, Hokkaido University, Muroran 051-0003, JapanResearch Center for Inland Seas, Kobe University, Kobe 657-8501, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Yamagishi
authors.affiliation: Research Center for Inland Seas, Kobe University, Kobe 657-8501, JapanMuroran Marine Station, Field Science Center for Northern Biosphere, Hokkaido University, Muroran 051-0003, JapanResearch Center for Inland Seas, Kobe University, Kobe 657-8501, Japan.
authors.forename: Taizo
authors.initials: T
authors.lastname: Motomura
authors.affiliation: Research Center for Inland Seas, Kobe University, Kobe 657-8501, JapanMuroran Marine Station, Field Science Center for Northern Biosphere, Hokkaido University, Muroran 051-0003, JapanResearch Center for Inland Seas, Kobe University, Kobe 657-8501, Japan.
authors.forename: Chikako
authors.initials: C
authors.lastname: Nagasato
authors.affiliation: Research Center for Inland Seas, Kobe University, Kobe 657-8501, JapanMuroran Marine Station, Field Science Center for Northern Biosphere, Hokkaido University, Muroran 051-0003, JapanResearch Center for Inland Seas, Kobe University, Kobe 657-8501, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kawai
keywords: Chrysophyceae; EGF-like motif; Ochromonas; polyclonal antibody; stramenopile; tubular mastigoneme