title: Effects of fetal bovine serum and estrus buffalo serum on maturation of buffalo (Bubalus bubalis) oocytes in vitro.
abstract: OBJECTIVE
  The aim was to assess the effects of fetal bovine serum (FBS) and estrus buffalo serum (EBS) on in vitro maturation rate of oocytes in buffalo.
  
  
  METHODS
  Maturation rate of oocytes was assessed in two maturation media supplemented with 20% FBS and EBS. Oocytes maturation rate was evaluated on the basis of cumulus cell expansion and extrusion of polar body after 24 h of in vitro culture in CO2 incubator.
  
  
  RESULTS
  The average percentage of in vitro matured oocytes in FBS was 83.80%, and EBS was 77.45%, respectively. The results revealed a significant (p<0.05) increase in maturation rate of oocytes in FBS than EBS.
  
  
  CONCLUSIONS
  Buffalo oocytes were better in vitro matured in FBS than EBS.
authors.affiliation: Department of Veterinary Physiology & Biochemistry, Vanbandhu College of Veterinary Science & Animal Husbandry, Navsari Agricultural University, Navsari - 396 450, Gujarat, India.
authors.forename: Gopal
authors.initials: G
authors.lastname: Puri
authors.affiliation: Department of Veterinary Physiology & Biochemistry, Vanbandhu College of Veterinary Science & Animal Husbandry, Navsari Agricultural University, Navsari - 396 450, Gujarat, India.
authors.forename: S S
authors.initials: SS
authors.lastname: Chaudhary
authors.affiliation: Department of Veterinary Physiology & Biochemistry, Vanbandhu College of Veterinary Science & Animal Husbandry, Navsari Agricultural University, Navsari - 396 450, Gujarat, India.
authors.forename: V K
authors.initials: VK
authors.lastname: Singh
authors.affiliation: Department of Veterinary Physiology & Biochemistry, Vanbandhu College of Veterinary Science & Animal Husbandry, Navsari Agricultural University, Navsari - 396 450, Gujarat, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Sharma
keywords: buffalo oocytes; estrus buffalo serum; fetal bovine serum; in vitro maturation