title: Rapid electrical immunoassay of the cardiac biomarker troponin I through dielectrophoretic concentration using imbedded electrodes.
abstract: Rapidity and high sensitivity are critical factors for the diagnoses of heart attacks, and cardiac troponin I (cTnI) is at present a clinical standard for its diagnosis. Here we report a rapid, label-free, and highly sensitive single-walled carbon nanotube (SWCNT) electrical immunosensor, featuring two pairs of electrodes. Two concentration electrodes (gaps: 25 and 80µm) and two detection electrodes (source and drain; gap: 20µm; width: 50µm) were used for dielectrophoretic concentration of cTnI on the SWCNT channels and resistance measurements of the dielectrophoresis (DEP)-concentrated cTnI, respectively. The two concentration electrodes were imbedded between upper and lower dielectric layers, facing each other, underneath the -COOH-functionalized SWCNT channels deposited between the detection electrodes. Therefore, the gap between these imbedded concentration electrodes can be reduced to maximize the electric field intensity for DEP-mediated concentration of cTnI, thereby greatly reducing the detection time (1min) and enhancing the limit of detection (0.7-0.8pgmL(-)(1)). Relative resistance changes of the SWCNTs were measured as cTnI concentration in Tris-Borate-EDTA (TBE; 0.0025×) and human serum diluted 500-fold with 0.0025× TBE decreased from 100ngmL(-)(1) to 1pgmL(-1), and they were shown to be linear with the logarithm of cTnI concentration (R(2)=0.99 and 0.97, respectively). These immunosensors also showed high specificity over another cardiac biomarker, myoglobin, TBE medium (0.0025×), and 500-fold diluted human serum. The DEP-capture of cTnI depended on the frequency of the applied electric field, demonstrating the qualitative nature of the real part of the Clausius-Mossotti factor for cTnI.
authors.affiliation: School of Mechanical and Nuclear Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea.
authors.forename: Abhinav
authors.initials: A
authors.lastname: Sharma
authors.affiliation: School of Mechanical and Nuclear Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea.
authors.forename: Chang-Ho
authors.initials: CH
authors.lastname: Han
authors.affiliation: School of Mechanical and Nuclear Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea; Department of Biomedical Engineering, UNIST, Ulsan 44919, Republic of Korea; School of Materials Science and Engineering, UNIST, Ulsan 44919, Republic of Korea. Electronic address: jjang@unist.ac.kr.
authors.forename: Jaesung
authors.initials: J
authors.lastname: Jang
mesh_terms: D015415:Biomarkers / Q000097:blood; D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation; D002318:Cardiovascular Diseases / Q000097:blood / Q000175:diagnosis*; D055664:Electrochemical Techniques / Q000191:economics / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D006801:Humans; D007118:Immunoassay / Q000191:economics / Q000295:instrumentation*; D057230:Limit of Detection; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D013997:Time Factors; D019210:Troponin I / Q000097:blood*
chemical_list: D015415:Biomarkers; D037742:Nanotubes, Carbon; D019210:Troponin I
keywords: Carbon nanotubes; Cardiac troponin I; Cardiovascular disease; Dielectrophoresis; Immunoassay