title: A sandwich dipstick assay for ATP detection based on split aptamer fragments.
abstract: Aptamer-based strip assay is an easy, highly efficient and low-cost detection method, which has been developed and easily applied to onsite detection. A new sensitive sandwich dipstick assay for adenosine triphosphate (ATP) detection was successfully developed based on specific recognition between split aptamer fragments and the target. In this method, the thiolated aptamer was first conjugated to the surface of gold nanoparticles (AuNPs), while the biotin-aptamer was immobilized on the surface of a nitrocellulose filter in the test line. In the presence of ATP, the thiol-aptamer/ATP/biotin-aptamer complexes were generated, which led to an obvious increase in optical signals at the test line. Under the optimal determination conditions, an excellent linear logarithmic response to the ATP concentration was obtained within the range of 0.5 μM to 5 mM. The limit of detection (LOD) of 0.5 μM was reached at a signal-to-noise ratio of 3. The dipstick assay showed a good average recovery of 96-108 % with the RSD of less than 20 % in urine samples. The proposed method exhibited high specificity against other nucleotides such as the uridine triphosphate (UTP), cytidine triphosphate (CTP), and guanosine triphosphate (GTP). The results indicated that the dipstick strip may be considered as an inexpensive screening tool for onsite ATP determination. Graphical Abstract A simple split aptamer fragments based sandwich-type dipstick assay was developed for ATP detection.
authors.affiliation: Institute of Quality Standards and Testing Technology for Agro-products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.|Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing, 100081, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Institute of Quality Standards and Testing Technology for Agro-products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.|Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing, 100081, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Institute of Quality Standards and Testing Technology for Agro-products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.|Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing, 100081, China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Yan
authors.affiliation: Institute of Quality Standards and Testing Technology for Agro-products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.|Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing, 100081, China.|College of Food Science and Technology, Hainan University, Haikou, Hainan, 570228, China.
authors.forename: Yafei
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Institute of Quality Standards and Testing Technology for Agro-products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.|Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing, 100081, China.|College of Food Science and Technology, Hainan University, Haikou, Hainan, 570228, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Yan
authors.affiliation: Institute of Quality Standards and Testing Technology for Agro-products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.|Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing, 100081, China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Bai
authors.affiliation: Institute of Quality Standards and Testing Technology for Agro-products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. ailiang.chen@gmail.com.|Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing, 100081, China. ailiang.chen@gmail.com.
authors.forename: Ailiang
authors.initials: A
authors.lastname: Chen
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D052157:Aptamers, Nucleotide / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D052156:SELEX Aptamer Technique / Q000295:instrumentation / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D052157:Aptamers, Nucleotide; D001710:Biotin; D006046:Gold; D000255:Adenosine Triphosphate
keywords: ATP; Dipstick strip; Gold nanoparticles; Split aptamer fragments