title: Therapeutic effects of tyroservatide on metastasis of lung cancer and its mechanism affecting integrin-focal adhesion kinase signal transduction.
abstract: Tyroservatide (YSV) can inhibit the growth and metastasis of mouse lung cancer significantly. This study investigated the therapeutic effects of tripeptide YSV on metastasis of human lung cancer cells and explored its possible mechanism that affects integrin-focal adhesion kinase (FAK) signal transduction in tumor cells. YSV significantly inhibited the adhesion and the invasion of highly metastatic human lung cancer cell lines 95D, A549, and NCI-H1299. In addition, YSV significantly inhibited phosphorylation of FAK Tyr397 and FAK Tyr576/577 in the 95D, A549, and NCI-H1299 human lung cancer cells in vitro. And the mRNA level and protein expression of FAK in these human lung cancer cells decreased at the same time. YSV also significantly inhibited mRNA and protein levels of integrin β1 and integrin β3 in the 95D, A549, and NCI-H1299 human lung cancer cells. Our research showed that YSV inhibited adhesion and invasion of human lung cancer cells and exhibited therapeutic effects on metastasis of lung cancer.
authors.affiliation: Department of Immunology, Tianjin Medical University, Tianjin, People's Republic of China.
authors.forename: Yu-ting
authors.initials: YT
authors.lastname: Huang
authors.affiliation: Department of Immunology, Tianjin Medical University, Tianjin, People's Republic of China.
authors.forename: Lan
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Immunology, Tianjin Medical University, Tianjin, People's Republic of China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Fu
authors.affiliation: Department of Immunology, Tianjin Medical University, Tianjin, People's Republic of China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Department of Immunology, Tianjin Medical University, Tianjin, People's Republic of China.
authors.forename: Xiao-meng
authors.initials: XM
authors.lastname: Song
authors.affiliation: Department of Immunology, Tianjin Medical University, Tianjin, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Jia
authors.affiliation: Department of Immunology, Tianjin Medical University, Tianjin, People's Republic of China.
authors.forename: Song
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Immunology, Tianjin Medical University, Tianjin, People's Republic of China.
authors.forename: Jin-ping
authors.initials: JP
authors.lastname: Li
authors.affiliation: Department of Immunology, Tianjin Medical University, Tianjin, People's Republic of China.
authors.forename: Zhi-feng
authors.initials: ZF
authors.lastname: Zhu
authors.affiliation: Department of Immunology, Tianjin Medical University, Tianjin, People's Republic of China; Shenzhen Kangzhe Pharmaceutical Co., Ltd., Shenzhen, People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Lin
authors.affiliation: Department of Immunology, Tianjin Medical University, Tianjin, People's Republic of China; Shenzhen Kangzhe Pharmaceutical Co., Ltd., Shenzhen, People's Republic of China.
authors.forename: Rong
authors.initials: R
authors.lastname: Lu
authors.affiliation: Department of Immunology, Tianjin Medical University, Tianjin, People's Republic of China; Key Laboratory of Immuno Microenvironment and Disease of the Educational Ministry of China, Tianjin Medical University, Tianjin, People's Republic of China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Yao
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000502:physiology*; D006801:Humans; D016023:Integrins / Q000037:antagonists & inhibitors / Q000502:physiology*; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000473:pathology; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009842:Oligopeptides / Q000494:pharmacology / Q000627:therapeutic use*; D015398:Signal Transduction / Q000502:physiology
chemical_list: D016023:Integrins; D009842:Oligopeptides; C510683:tripeptide tyroservatide; D051416:Focal Adhesion Protein-Tyrosine Kinases; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: FAK; MMP-2; MMP-9; focal adhesion kinase; integrin; tyroservatide