title: The Three-Dimensional Culture System with Matrigel and Neurotrophic Factors Preserves the Structure and Function of Spiral Ganglion Neuron In Vitro.
abstract: Whole organ culture of the spiral ganglion region is a resourceful model system facilitating manipulation and analysis of live sprial ganglion neurons (SGNs). Three-dimensional (3D) cultures have been demonstrated to have many biomedical applications, but the effect of 3D culture in maintaining the SGNs structure and function in explant culture remains uninvestigated. In this study, we used the matrigel to encapsulate the spiral ganglion region isolated from neonatal mice. First, we optimized the matrigel concentration for the 3D culture system and found the 3D culture system protected the SGNs against apoptosis, preserved the structure of spiral ganglion region, and promoted the sprouting and outgrowth of SGNs neurites. Next, we found the 3D culture system promoted growth cone growth as evidenced by a higher average number and a longer average length of filopodia and a larger growth cone area. 3D culture system also significantly elevated the synapse density of SGNs. Last, we found that the 3D culture system combined with neurotrophic factors had accumulated effects in promoting the neurites outgrowth compared with 3D culture or NFs treatment only groups. Together, we conclude that the 3D culture system preserves the structure and function of SGN in explant culture.
authors.affiliation: Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250022, China; Shandong Provincial Key Laboratory of Otology, Jinan 250022, China.
authors.forename: Gaoying
authors.initials: G
authors.lastname: Sun
authors.affiliation: Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250022, China; Shandong Provincial Key Laboratory of Otology, Jinan 250022, China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Liu
authors.affiliation: Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250022, China.
authors.forename: Zhaomin
authors.initials: Z
authors.lastname: Fan
authors.affiliation: Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250022, China.
authors.forename: Daogong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250022, China.
authors.forename: Yuechen
authors.initials: Y
authors.lastname: Han
authors.affiliation: Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250022, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Xu
authors.affiliation: Key Laboratory for Developmental Genes and Human Disease, Ministry of Education, Institute of Life Sciences, Southeast University, Nanjing 210096, China; Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China.
authors.forename: Jieyu
authors.initials: J
authors.lastname: Qi
authors.affiliation: Key Laboratory for Developmental Genes and Human Disease, Ministry of Education, Institute of Life Sciences, Southeast University, Nanjing 210096, China; Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Zhang
authors.affiliation: College of Medicine, The University of Tennessee Health Science Center, Memphis, TN 38163, USA.
authors.forename: Bradley T
authors.initials: BT
authors.lastname: Gao
authors.affiliation: Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250022, China; Shandong Provincial Key Laboratory of Otology, Jinan 250022, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Bai
authors.affiliation: Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250022, China; Shandong Provincial Key Laboratory of Otology, Jinan 250022, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory for Developmental Genes and Human Disease, Ministry of Education, Institute of Life Sciences, Southeast University, Nanjing 210096, China; Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China.
authors.forename: Renjie
authors.initials: R
authors.lastname: Chai
authors.affiliation: Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250022, China; Shandong Provincial Key Laboratory of Otology, Jinan 250022, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D003094:Collagen; D004338:Drug Combinations; D007797:Laminin; D051379:Mice; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D011509:Proteoglycans; D013136:Spiral Ganglion / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D013569:Synapses / Q000187:drug effects / Q000502:physiology
chemical_list: D004338:Drug Combinations; D007797:Laminin; D009414:Nerve Growth Factors; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen