title: Hyperbaric oxygen therapy improves colorectal anastomotic healing.
abstract: OBJECTIVE
  Hyperbaric oxygen treatment (HBOT) has been found to improve the healing of poorly oxygenated tissues. This study aimed to investigate the influence of HBOT on the healing in ischemic colorectal anastomosis.
  
  
  METHODS
  Forty Wistar rats were randomly divided into a treatment group that received HBOT for 10 consecutive days (7 days before and 3 days after surgery), or in a control group, which did not receive the therapy. Colectomy with an ischemic anastomosis was performed in all rats. In each group, the rats were followed for 3 or 7 days after surgery to determine the influence of HBOT on anastomotic healing.
  
  
  RESULTS
  Five rats from each group died during follow-up. No anastomotic dehiscence was seen in the HBOT group, compared to 37.5 % and 28.6 % dehiscence in the control group on postoperative day (POD) 3 and 7, respectively. The HBOT group had a significantly higher bursting pressure (130.9 ± 17.0 mmHg) than the control group (88.4 ± 46.7 mmHg; p = 0.03) on POD 3. On POD 3 and POD 7, the adhesion severity was significantly higher in the control groups than in the HBOT groups (p < 0.005). Kidney function (creatinine level) of the HBOT group was significantly better than of the control group on POD 7 (p = 0.001). Interestingly, a significantly higher number of CD206+ cells (marker for type 2 macrophages) was observed in the HBOT group at the anastomotic area on POD 3.
  
  
  CONCLUSIONS
  Hyperbaric oxygen enhanced the healing of ischemic anastomoses in rats and improved the postoperative kidney function.
authors.affiliation: Department of Surgery, Laboratory of Experimental Surgery, Erasmus MC, University Medical Center, Room Ee-173 Postbus 2040, 3000 CA, Rotterdam, The Netherlands.
authors.forename: G S A
authors.initials: GSA
authors.lastname: Boersema
authors.affiliation: Department of Surgery, Laboratory of Experimental Surgery, Erasmus MC, University Medical Center, Room Ee-173 Postbus 2040, 3000 CA, Rotterdam, The Netherlands. wuzhouqiao@gmail.com.|Department of Gastrointestinal Surgery, Peking University Cancer Hospital and Institute, Beijing, China. wuzhouqiao@gmail.com.
authors.forename: Z
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Surgery, Laboratory of Experimental Surgery, Erasmus MC, University Medical Center, Room Ee-173 Postbus 2040, 3000 CA, Rotterdam, The Netherlands.
authors.forename: L F
authors.initials: LF
authors.lastname: Kroese
authors.affiliation: Department of Surgery, Laboratory of Experimental Surgery, Erasmus MC, University Medical Center, Room Ee-173 Postbus 2040, 3000 CA, Rotterdam, The Netherlands.|Department of Surgery, Amsterdam Medical Center, Amsterdam, The Netherlands.
authors.forename: S
authors.initials: S
authors.lastname: Vennix
authors.affiliation: Department of Orthopaedics, Erasmus MC, University Medical Center, Rotterdam, The Netherlands.
authors.forename: Y M
authors.initials: YM
authors.lastname: Bastiaansen-Jenniskens
authors.affiliation: Department of Plastic and Reconstructive Surgery, Erasmus MC, University Medical Center, Rotterdam, The Netherlands.
authors.forename: J W
authors.initials: JW
authors.lastname: van Neck
authors.affiliation: Department of Pathology, Erasmus MC, University Medical Center, Rotterdam, The Netherlands.
authors.forename: K H
authors.initials: KH
authors.lastname: Lam
authors.affiliation: Department of Neuroscience, Erasmus University Medical Center, Rotterdam, The Netherlands.
authors.forename: G J
authors.initials: GJ
authors.lastname: Kleinrensink
authors.affiliation: Department of Neuroscience, Erasmus University Medical Center, Rotterdam, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Jeekel
authors.affiliation: Department of Surgery, Laboratory of Experimental Surgery, Erasmus MC, University Medical Center, Room Ee-173 Postbus 2040, 3000 CA, Rotterdam, The Netherlands.
authors.forename: J F
authors.initials: JF
authors.lastname: Lange
mesh_terms: D018784:Abdominal Abscess / Q000097:blood / Q000150:complications / Q000209:etiology; D000714:Anastomosis, Surgical / Q000009:adverse effects; D057868:Anastomotic Leak / Q000097:blood / Q000209:etiology; D000818:Animals; D002452:Cell Count; D003106:Colon / Q000601:surgery*; D003404:Creatinine / Q000097:blood; D006931:Hyperbaric Oxygenation; D008264:Macrophages / Q000473:pathology; D008297:Male; D017208:Rats, Wistar; D012007:Rectum / Q000601:surgery*; D013529:Surgical Wound Dehiscence / Q000097:blood / Q000150:complications / Q000209:etiology; D000267:Tissue Adhesions / Q000097:blood / Q000150:complications / Q000473:pathology; D014945:Wound Healing
chemical_list: D003404:Creatinine
keywords: Anastomotic healing; Animal model; Hyperbaric oxygen therapy; Perfusion