title: Preliminary characterization of biosurfactant produced by a PAH-degrading Paenibacillus sp. under thermophilic conditions.
abstract: The capacities of a biosurfactant producing and polycyclic aromatic hydrocarbon (PAH) utilizing bacterium, namely, strain 1C, isolated from an Algerian contaminated soil, were investigated. Strain 1C belonged to the Paenibacillus genus and was closely related to the specie Paenibacillus popilliae, with 16S rRNA gene sequence similarity of 98.4 %. It was able to produce biosurfactant using olive oil as substrate. The biosurfactant production was shown by surface tension (32.6 mN/m) after 24 h of incubation at 45 °C and 150 rpm. The biosurfactant(s) retained its properties during exposure to elevated temperatures (70 °C), relatively high salinity (20 % NaCl), and a wide range of pH values (2-10). The infrared spectroscopy (FTIR) revealed that its chemical structure belonged to lipopeptide class. The critical micelle concentration (CMC) of this biosurfactant was about 0.5 g/l with 29.4 mN/m. In addition, the surface active compound(s) produced by strain 1C enhanced PAH solubility and showed a significant antimicrobial activity against pathogens. In addition to its biosurfactant production, strain 1C was shown to be able to utilize PAHs as the sole carbon and energy sources. Strain 1C as hydrocarbonoclastic bacteria and its interesting surface active agent may be used for cleaning the environments polluted with polyaromatic hydrocarbons.
authors.affiliation: Laboratory of Natural Products Chemistry and Biomolecules (LNSCB), University of Saad Dahlab-Blida 1, P.O. Box 270, 09000, Blida, Algeria. fatmazohra-env@hotmail.fr.|National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd. Amirouche, P.O. Box 67, Bousmail, W. Tipaza, Algeria. fatmazohra-env@hotmail.fr.
authors.forename: Fatma Zohra
authors.initials: FZ
authors.lastname: Mesbaiah
authors.affiliation: Laboratory of Natural Products Chemistry and Biomolecules (LNSCB), University of Saad Dahlab-Blida 1, P.O. Box 270, 09000, Blida, Algeria.|Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, PO Box 1177, 3018, Sfax, Tunisia.
authors.forename: Kamel
authors.initials: K
authors.lastname: Eddouaouda
authors.affiliation: Laboratory of Natural Products Chemistry and Biomolecules (LNSCB), University of Saad Dahlab-Blida 1, P.O. Box 270, 09000, Blida, Algeria.|National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd. Amirouche, P.O. Box 67, Bousmail, W. Tipaza, Algeria.
authors.forename: Abdelmalek
authors.initials: A
authors.lastname: Badis
authors.affiliation: Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, PO Box 1177, 3018, Sfax, Tunisia.
authors.forename: Alif
authors.initials: A
authors.lastname: Chebbi
authors.affiliation: Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, PO Box 1177, 3018, Sfax, Tunisia.
authors.forename: Dorra
authors.initials: D
authors.lastname: Hentati
authors.affiliation: Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, PO Box 1177, 3018, Sfax, Tunisia.
authors.forename: Sami
authors.initials: S
authors.lastname: Sayadi
authors.affiliation: Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, PO Box 1177, 3018, Sfax, Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Chamkha
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D000935:Antifungal Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D001673:Biodegradation, Environmental; D008826:Microbial Sensitivity Tests; D058889:Molecular Typing; D000069463:Olive Oil / Q000378:metabolism; D056507:Paenibacillus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010802:Phylogeny; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013501:Surface-Active Agents / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D014873:Water Pollutants / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D000069463:Olive Oil; D011084:Polycyclic Aromatic Hydrocarbons; D012336:RNA, Ribosomal, 16S; D013501:Surface-Active Agents; D014873:Water Pollutants
keywords: Bioremediation; Biosurfactant; Characterization; PAHs; Paenibacillus