title: Fos expression in catecholaminergic medullary neurons induced by chemical stimulation of stomach projecting to the paraventricular nucleus of the hypothalamus in rats.
abstract: OBJECTIVE
  To determine whether medullary catecholaminergic neurons expressing Fos induced by chemical stimulation of the stomach project to the paraventricular nucleus of hypothalamus (PVH) in rats.
  
  
  METHODS
  Horseradish peroxidase (HRP) was introduced stereotaxically into the PVH of rats. Histochemical analysis of coronal sections through the medulla were analyzed using triple-label immunohistochemistry to identify cells that were retrogradely labeled with HRP, Fos (ABC method), and tyrosin hydroxylase (TH) (PAP method).
  
  
  RESULTS
  Seven kinds of labeled neurons were found in the nucleus tractus solitarii (NTS), the ventrolateral medulla (VLM) and the reticular formation (RF) of the medulla: Fos-like immunoreactive (FosLI) neurons, TH-like immunoreactive (TH-LI) neurons and HRP retrogradely single-labeled neurons, FosLI/HRP, FosLI/TH-LI and HRP/TH-LI double-labeled neurons, and FosLI/HRP/TH-LI triple-labeled neurons.
  
  
  CONCLUSIONS
  Ascending projections from the NTS, VLM and RF to the PVH might be involved in the transmitting process of visceral noxious stimulation.
authors.affiliation: Yuan-Xiang Dong, Kang-Hui Xiong, Zhi-Ren Rao, Ji-Wu Shi, Department of Anatomy, The Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China.
authors.forename: Y X
authors.initials: YX
authors.lastname: Dong
authors.affiliation: Yuan-Xiang Dong, Kang-Hui Xiong, Zhi-Ren Rao, Ji-Wu Shi, Department of Anatomy, The Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China.
authors.forename: K H
authors.initials: KH
authors.lastname: Xiong
authors.affiliation: Yuan-Xiang Dong, Kang-Hui Xiong, Zhi-Ren Rao, Ji-Wu Shi, Department of Anatomy, The Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China.
authors.forename: Z R
authors.initials: ZR
authors.lastname: Rao
authors.affiliation: Yuan-Xiang Dong, Kang-Hui Xiong, Zhi-Ren Rao, Ji-Wu Shi, Department of Anatomy, The Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China.
authors.forename: J W
authors.initials: JW
authors.lastname: Shi
keywords: Immunochemistry; Medulla oblongata; Neurons; Paraventricular hypothalamic nucleus