title: Melatonin can Ameliorate Radiation-Induced Oxidative Stress and Inflammation-Related Deterioration of Bone Quality in Rat Femur.
abstract: The aim of the present study was to evaluate the radioprotective effects of melatonin on the biomechanical properties of bone in comparison to amifostine (WR-2721). Forty Sprague Dawley rats were divided equally into 5 groups namely; control (C), irradiation (R; single dose of 50 Gy), irradiation + WR-2721 (R + WR-2721; irradiation + 200 mg/kg WR-2721) radiation + melatonin 25 mg/kg (R + M25; irradiation + 25 mg/kg melatonin), and radiation + melatonin 50 mg/kg (R + M50; irradiation + 50 mg/kg melatonin). In order to measure extrinsic (organ-level mechanical properties of bone; the ultimate strength, deformation, stiffness, energy absorption capacity) and intrinsic (tissue-level mechanical properties of bone; ultimate stress, ultimate strain, elastic modulus, toughness) features of the bone, a three-point bending (TPB) test was performed for biomechanical evaluation. In addition, a bone mineral density (BMD) test was carried out. The BMD and extrinsic properties of the diaphyseal femur were found to be significantly higher in the R + M25 group than in group R (p < 0.05). A significant increase was observed in R + M50 (p < 0.05) in comparison to group R in the cross-sectional area of the femoral shaft and elastic modulus parameter. The protective effect of melatonin was similar to that of WR-2721. Thus, biomechanical quality of irradiated bone can be ameliorated by free radical scavenger melatonin.
authors.affiliation: Department of Biophysics, Faculty of Medicine, Gaziantep University, Gaziantep, TR-27310, Turkey.
authors.forename: Zelal Ünlü
authors.initials: ZÜ
authors.lastname: Çakir
authors.affiliation: Department of Biophysics, Faculty of Medicine, Gaziantep University, Gaziantep, TR-27310, Turkey. cdemirel27@gmail.com.
authors.forename: Can
authors.initials: C
authors.lastname: Demirel
authors.affiliation: Department of Radiation Oncology, Kolan International Hospital, Istanbul, TR-34000, Turkey.
authors.forename: Sevil Cagiran
authors.initials: SC
authors.lastname: Kilciksiz
authors.affiliation: Department of Biophysics, Faculty of Medicine, Gaziosmanpaşa University, Tokat, TR-60000, Turkey.
authors.forename: Serkan
authors.initials: S
authors.lastname: Gürgül
authors.affiliation: Department of Radiation Oncology, Faculty of Medicine, Dicle University, Diyarbakır, TR-33169, Turkey.
authors.forename: S Burhanedtin
authors.initials: SB
authors.lastname: Zincircioğlu
authors.affiliation: Department of Biophysics, Faculty of Medicine, Mersin University, Mersin, TR-33169, Turkey.
authors.forename: Nurten
authors.initials: N
authors.lastname: Erdal
mesh_terms: D000818:Animals; D015519:Bone Density / Q000187:drug effects; D001842:Bone and Bones / Q000187:drug effects / Q000503:physiopathology*; D018483:Diaphyses / Q000187:drug effects; D004307:Dose-Response Relationship, Radiation; D005269:Femur / Q000187:drug effects / Q000503:physiopathology; D005720:Gamma Rays / Q000009:adverse effects; D007249:Inflammation / Q000150:complications*; D055595:Mechanical Phenomena / Q000187:drug effects / Q000528:radiation effects; D008550:Melatonin / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D008550:Melatonin
keywords: WR-2721; antioxidants; biomechanics; bone quality; inflammation; melatonin; radioprotection; radiotherapy