title: Elevated expression of circulating miR876-5p is a specific response to severe EV71 infections.
abstract: Human enterovirus 71 (EV71) is a major causative agent of hand, foot, and, mouth disease, accounting for more than 65% of recent outbreaks. Following enteroviral infection, the host responses are crucial indicators for the development of a diagnosis regarding the clinical severity of EV71 infections. In this study, we implemented NanoString nCounter technology to characterize the responses of serum microRNA (miRNA) profiles to various EV71 infection diseases. Upon EV71 infection, 44 miRNAs were observed in patients with EV71 infections, with at least a 2-fold elevation and 133 miRNAs with a 2-fold reduction compared with the same miRNAs in healthy controls. Further detailed work with miR876-5p, a 9.5-fold change of upregulated miR-876-5p expression was observed in cases with severe EV71 symptoms, revealed that in vitro and in vivo knockdown of miR876-5p reduced viral RNA in cultured cells, and attenuated the severity of symptoms in EV71-infected mice. Altogether, we demonstrated that the elevated expression of circulating miR876-5p is a specific response to severe EV71 infections.
authors.affiliation: Department of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, 33302, Taiwan.
authors.forename: Robert Y L
authors.initials: RYL
authors.lastname: Wang
authors.affiliation: Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
authors.forename: Kuo-Feng
authors.initials: KF
authors.lastname: Weng
authors.affiliation: Division of Pediatric Infectious Diseases, Department of Pediatrics, Chang Gung Memorial and Children's Hospital, Linkuo, Taiwan.
authors.forename: Yhu-Chering
authors.initials: YC
authors.lastname: Huang
authors.affiliation: Division of Pediatric Infectious Diseases, Department of Pediatrics, Chang Gung Memorial and Children's Hospital, Linkuo, Taiwan.
authors.forename: Chih-Jung
authors.initials: CJ
authors.lastname: Chen
mesh_terms: D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D002648:Child; D002675:Child, Preschool; D004285:Dogs; D029821:Enterovirus A, Human / Q000235:genetics* / Q000502:physiology; D004769:Enterovirus Infections / Q000097:blood / Q000235:genetics* / Q000821:virology; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D055785:Gene Knockdown Techniques; D053263:Gene Regulatory Networks; D054884:Host-Pathogen Interactions / Q000235:genetics; D006801:Humans; D007223:Infant; D061985:Madin Darby Canine Kidney Cells; D008297:Male; D008813:Mice, Inbred ICR; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: C000598700:MIRN876 microRNA, human; D035683:MicroRNAs; D012367:RNA, Viral