title: Coexpression network analysis of the genes regulated by two types of resistance responses to powdery mildew in wheat.
abstract: Powdery mildew disease caused by Blumeria graminis f. sp. tritici (Bgt) inflicts severe economic losses in wheat crops. A systematic understanding of the molecular mechanisms involved in wheat resistance to Bgt is essential for effectively controlling the disease. Here, using the diploid wheat Triticum urartu as a host, the genes regulated by immune (IM) and hypersensitive reaction (HR) resistance responses to Bgt were investigated through transcriptome sequencing. Four gene coexpression networks (GCNs) were developed using transcriptomic data generated for 20 T. urartu accessions showing IM, HR or susceptible responses. The powdery mildew resistance regulated (PMRR) genes whose expression was significantly correlated with Bgt resistance were identified, and they tended to be hubs and enriched in six major modules. A wide occurrence of negative regulation of PMRR genes was observed. Three new candidate immune receptor genes (TRIUR3_13045, TRIUR3_01037 and TRIUR3_06195) positively associated with Bgt resistance were discovered. Finally, the involvement of TRIUR3_01037 in Bgt resistance was tentatively verified through cosegregation analysis in a F2 population and functional expression assay in Bgt susceptible leaf cells. This research provides insights into the global network properties of PMRR genes. Potential molecular differences between IM and HR resistance responses to Bgt are discussed.
authors.affiliation: The State Key Laboratory of Plant Cell and chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Juncheng
authors.initials: J
authors.lastname: Zhang
authors.affiliation: The Collaborative Innovation Center for Grain Crops, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Hongyuan
authors.initials: H
authors.lastname: Zheng
authors.affiliation: The State Key Laboratory of Plant Cell and chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Li
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Li
authors.affiliation: The State Key Laboratory of Plant Cell and chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: The State Key Laboratory of Plant Cell and chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Huanju
authors.initials: H
authors.lastname: Qin
authors.affiliation: The State Key Laboratory of Plant Cell and chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Lingli
authors.initials: L
authors.lastname: Dong
authors.affiliation: The State Key Laboratory of Plant Cell and chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.|The Collaborative Innovation Center for Grain Crops, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Daowen
authors.initials: D
authors.lastname: Wang
mesh_terms: D001203:Ascomycota / Q000502:physiology*; D060467:Disease Resistance / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D017343:Genes, Plant; D018515:Plant Leaves / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D018749:RNA, Plant / Q000096:biosynthesis / Q000235:genetics; D059010:Single-Cell Analysis; D059467:Transcriptome; D014908:Triticum / Q000235:genetics* / Q000276:immunology / Q000382:microbiology
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant