title: Relationships between leptin, KiSS-1/GPR54 expression and TSH secretion from pituitary cells of pubertal ewes in vitro.
abstract: Kisspeptin and leptin play a crucial role in the puberty of sheep as they initiate the activity of hypothalamic-pituitary-ovarian axis. Also hormones of thyrotropic axis are probably involved in this process. The aim of study was to analyze the impact of leptin on kisspeptin-10 secretion as well as kisspeptin-1 and G protein-coupled receptor (GPR54) mRNA expression in pituitary cells of pubertal ewes in vitro. The influence of kisspeptin on TSH secretion was also examined. Cells were cultured in McCoy's 5A medium without hormones; with 10(-10)-10(-5)M of leptin; with 10(-11)-10(-5)M of kisspeptin-10; with peptide 234 (10(-7)M, antagonist of GPR54) or 10(-11)-10(-5)M of kisspeptin-10 and peptide 234. Then, kisspeptin-10 and TSH secretion as well as KiSS-1 and GPR54 expression were analyzed. We found that leptin directly affected kisspeptin-10 secretion and kisspeptin-1/GPR54 expression in pituitary cells of pubertal ewes. Kisspeptin-10 did not change TSH secretion, except exerting a short-term influence after 2h.
authors.affiliation: Department of Pathophysiology, Chair of Preclinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland. Electronic address: radwanek@autograf.pl.
authors.forename: Paulina
authors.initials: P
authors.lastname: Radwańska
authors.affiliation: Department of Pathophysiology, Chair of Preclinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland.
authors.forename: Urszula
authors.initials: U
authors.lastname: Kosior-Korzecka
mesh_terms: D000818:Animals; D005260:Female; D059648:Kisspeptins / Q000235:genetics* / Q000378:metabolism; D020738:Leptin / Q000502:physiology*; D010902:Pituitary Gland / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000378:metabolism; D012756:Sheep / Q000235:genetics* / Q000378:metabolism; D013972:Thyrotropin / Q000378:metabolism*
chemical_list: D059648:Kisspeptins; D020738:Leptin; D043562:Receptors, G-Protein-Coupled; D013972:Thyrotropin
keywords: Kisspeptin; Leptin; Pituitary cells in vitro; Pubertal ewes; Thyroid-stimulating hormone