title: Evaluating the diagnostic and prognostic value of circulating cathepsin S in gastric cancer.
abstract: To evaluate whether serum Cathepsin S (Cat S) could serve as a biomarker for the diagnosis and prognosis of gastric cancer (GC), Enzyme-linked immuno sorbent assay (ELISA) was used to detect serum Cat S in 496 participants including healthy controls and patients with benign gastric diseases, gastric cancer, esophageal cancer, liver cancer, colorectal cancer, nasopharyngeal cancer and lung cancer. The levels of serum Cat S were significantly increased in cancer patients, especially in GC patients. The qRT-PCR, Western blotting, and immunohistochemical staining revealed the overexpression of Cat S in GC cell lines and tissues. The diagnostic value of serum Cat S for GC patients from controls resulted in an AUC of 0.803 with a sensitivity of 60.7% and a specificity of 90.0%. Moreover, the levels of serum Cat S were associated with GC tumor volume, lymphoid nodal status, metastasis status, and stages. Moreover, the patients with high levels of serum Cat S had a poorer overall survival. Univariate analysis revealed Cat S expression was a prognostic factor. The knockdown of Cat S significantly suppressed the migration and invasion of GC cells. This study suggested serum Cat S may be a potential biomarker for the diagnosis and prognosis of GC.
authors.affiliation: State Key Laboratory of Oncology in Southern China, Sun Yat-sen University Cancer Center, Guangzhou, China.|Department of Clinical Laboratory Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.
authors.forename: Wan-Li
authors.initials: WL
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Oncology in Southern China, Sun Yat-sen University Cancer Center, Guangzhou, China.|Department of Clinical Laboratory Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Microbial and Biochemical Pharmacy, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Oncology in Southern China, Sun Yat-sen University Cancer Center, Guangzhou, China.|Department of Clinical Laboratory Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.
authors.forename: Yi-Jun
authors.initials: YJ
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Oncology in Southern China, Sun Yat-sen University Cancer Center, Guangzhou, China.|Department of Clinical Laboratory Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Xing
authors.affiliation: State Key Laboratory of Oncology in Southern China, Sun Yat-sen University Cancer Center, Guangzhou, China.|Department of Clinical Laboratory Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.
authors.forename: Pei-Dong
authors.initials: PD
authors.lastname: Chi
authors.affiliation: State Key Laboratory of Oncology in Southern China, Sun Yat-sen University Cancer Center, Guangzhou, China.|Department of Clinical Laboratory Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Oncology in Southern China, Sun Yat-sen University Cancer Center, Guangzhou, China.|Department of Clinical Laboratory Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Xue
authors.affiliation: State Key Laboratory of Oncology in Southern China, Sun Yat-sen University Cancer Center, Guangzhou, China.|Department of Clinical Laboratory Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.
authors.forename: Yan-Zhen
authors.initials: YZ
authors.lastname: Lai
authors.affiliation: Department of Nasopharyngeal Carcinoma, Sun Yat-sen University of Cancer Center, Guangzhou, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Guo
authors.affiliation: Department of Microbial and Biochemical Pharmacy, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood*; D002403:Cathepsins / Q000097:blood*; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012372:ROC Curve; D012680:Sensitivity and Specificity; D013274:Stomach Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000473:pathology; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D002403:Cathepsins; C028217:cathepsin S
keywords: Cat S; ELISA; diagnosis and prognostic biomarker; gastric cancer