title: Sphingosine inhibits the sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA) activity.
abstract: The increase in the intracellular Ca(2+) concentration ([Ca(2+)]i) is the key variable for many different processes, ranging from regulation of cell proliferation to apoptosis. In this work we demonstrated that the sphingolipid sphingosine (Sph) increases the [Ca(2+)]i by inhibiting the sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA), in a similar manner to thapsigargin (Tg), a specific inhibitor of this Ca(2+) pump. The results showed that addition of sphingosine produced a release of Ca(2+) from the endoplasmic reticulum followed by a Ca(2+) entrance from the outside mileu. The results presented in this work support that this sphingolipid could control the activity of the SERCA, and hence sphingosine may participate in the regulation of [Ca(2+)]I in mammalian cells.
authors.affiliation: Instituto de Estudios Avanzados (IDEA), Caracas, Venezuela; Instituto de Biología Experimental, Facultad de Ciencias, Universidad Central de Venezuela (UCV), Caracas, Venezuela. Electronic address: gbenaim@idea.gob.ve.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Benaim
authors.affiliation: Facultad de Farmacia, Universidad Central de Venezuela (UCV), Caracas, Venezuela. Electronic address: adriana.pimentel@ucv.ve.
authors.forename: Adriana A
authors.initials: AA
authors.lastname: Pimentel
authors.affiliation: Facultad de Ciencias, Universidad de Carabobo, Valencia, Venezuela.
authors.forename: Pimali
authors.initials: P
authors.lastname: Felibertt
authors.affiliation: Instituto de Biología Experimental, Facultad de Ciencias, Universidad Central de Venezuela (UCV), Caracas, Venezuela.
authors.forename: Adriana
authors.initials: A
authors.lastname: Mayora
authors.affiliation: Instituto Pasteur de Montevideo, Montevideo, Uruguay.
authors.forename: Laura
authors.initials: L
authors.lastname: Colman
authors.affiliation: Instituto de Biología Experimental, Facultad de Ciencias, Universidad Central de Venezuela (UCV), Caracas, Venezuela.
authors.forename: Felipe
authors.initials: F
authors.lastname: Sojo
authors.affiliation: Instituto de Inmunología, Universidad Central de Venezuela (UCV), Caracas, Venezuela; Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Venezuela.
authors.forename: Héctor
authors.initials: H
authors.lastname: Rojas
authors.affiliation: Instituto de Inmunología, Universidad Central de Venezuela (UCV), Caracas, Venezuela.
authors.forename: Juan B
authors.initials: JB
authors.lastname: De Sanctis
mesh_terms: D002118:Calcium / Q000378:metabolism*; D045744:Cell Line, Tumor; D004721:Endoplasmic Reticulum / Q000378:metabolism; D004789:Enzyme Activation; D006801:Humans; D009369:Neoplasms / Q000378:metabolism*; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000378:metabolism*; D013110:Sphingosine / Q000378:metabolism*
chemical_list: D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D013110:Sphingosine; D002118:Calcium
keywords: Ca(+2); SERCA; Sarco(endo)plasmic reticulum Ca(+2)-ATPase; Sphingolipid; Sphingosine