title: Composite poly(l-lactic-acid)/silk fibroin scaffold prepared by electrospinning promotes chondrogenesis for cartilage tissue engineering.
abstract: Nanofibrous materials produced by electrospinning have attracted considerable attention from researchers in regenerative medicine. A combination of nanofibrous scaffold and chondrocytes is considered promising for repair of cartilage defect or damage. In the present study, we fabricated a poly(l-lactic-acid) (PLLA)/silk fibroin (SF) nanofibrous scaffold by electrospinning and evaluated its chondrogenic potential. The PLLA/SF nanofibers were characterized for diameter, surface wettability, swelling ratio, and tensile strength. Throughin vitroexperiments, PLLA/SF scaffold-chondrocyte interactions were investigated relative to the unmodified PLLA scaffold with regard to cellular adhesion, spreading, and proliferation by scanning electron microscopy and confocal laser scanning microscopy, and through analyses of DNA, sulfated glycosaminoglycan, and collagen. In addition, hematoxylin-eosin and Alcian blue-nuclear fast red staining were used to observe growth of chondrocytes, and secretion and distribution of cartilage-specific extracellular matrices in the scaffolds. Expressions of cartilage-related genes (collagen II, aggrecan, sox9, collagen I, and collagen X) were detected by real-time quantitative PCR. The PLLA/SF scaffold had better hydrophilicity, and could support chondrocytes adhesion and spreading more effectively than the unmodified PLLA scaffold. Chondrocytes secreted more cartilage-specific extracellular matrices and maintained their phenotype on the PLLA/SF scaffold. So it is concluded that the PLLA/SF scaffold is more conducive toin vitroformation of cartilage-like new tissues than the unmodified PLLA scaffold, and may be a promising material in cartilage tissue engineering.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Jilin University, Changchun, China Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun, China.
authors.forename: Zhengqiang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Stomatology, School of Medicine, Yanbian University, Yanji, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.affiliation: College of Chemistry, Jilin University, Changchun, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Yang
authors.affiliation: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Jilin University, Changchun, China Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Jilin University, Changchun, China Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: You
authors.affiliation: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Jilin University, Changchun, China Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun, China.
authors.forename: Jiale
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Jilin University, Changchun, China Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun, China.
authors.forename: Zilin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Jilin University, Changchun, China Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun, China hbing@jlu.edu.cn.
authors.forename: Bing
authors.initials: B
authors.lastname: Han
mesh_terms: D000818:Animals; D002356:Cartilage / Q000166:cytology* / Q000254:growth & development; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology; D020219:Chondrogenesis; D005349:Fibroins / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011091:Polyesters / Q000737:chemistry*; D011817:Rabbits; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011091:Polyesters; C033616:poly(lactide); D005349:Fibroins
keywords: Electrospinning; cartilage tissue engineering; chondrocytes; nanofiber; poly-(l-lactic-acid); scaffold; silk fibroin