title: Calcium/Cobalt Alginate Beads as Functional Scaffolds for Cartilage Tissue Engineering.
abstract: Articular cartilage is a highly organized tissue with complex biomechanical properties. However, injuries to the cartilage usually lead to numerous health concerns and often culminate in disabling symptoms, due to the poor intrinsic capacity of this tissue for self-healing. Although various approaches are proposed for the regeneration of cartilage, its repair still represents an enormous challenge for orthopedic surgeons. The field of tissue engineering currently offers some of the most promising strategies for cartilage restoration, in which assorted biomaterials and cell-based therapies are combined to develop new therapeutic regimens for tissue replacement. The current study describes the in vitro behavior of human adipose-derived mesenchymal stem cells (hADSCs) encapsulated within calcium/cobalt (Ca/Co) alginate beads. These novel chondrogenesis-promoting scaffolds take advantage of the synergy between the alginate matrix and Co(+2) ions, without employing costly growth factors (e.g., transforming growth factor betas (TGF-βs) or bone morphogenetic proteins (BMPs)) to direct hADSC differentiation into cartilage-producing chondrocytes.
authors.affiliation: Department of Biomedical and Neuromotor Sciences, University of Bologna, 40126 Bologna, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Focaroli
authors.affiliation: Department of Biomedical and Neuromotor Sciences, University of Bologna, 40126 Bologna, Italy.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Teti
authors.affiliation: Department of Biomedical and Neuromotor Sciences, University of Bologna, 40126 Bologna, Italy.
authors.forename: Viviana
authors.initials: V
authors.lastname: Salvatore
authors.affiliation: Department of Pharmacy and Biotechnology, University of Bologna, 40126 Bologna, Italy.
authors.forename: Isabella
authors.initials: I
authors.lastname: Orienti
authors.affiliation: Department of Biomedical and Neuromotor Sciences, University of Bologna, 40126 Bologna, Italy.
authors.forename: Mirella
authors.initials: M
authors.lastname: Falconi