title: Bioassay-Guided Isolation of Compounds from Datura stramonium with TRAIL-Resistance Overcoming Activity.
abstract: TRAIL is a potent inducer of apoptosis in most cancer cells, but not in normal cells, and therefore has deserved intense interest as a promising agent for cancer therapy. In the search for bioactive natural products for overcoming TRAIL-resistance, we previously reported a number of active compounds. In our screening program on natural resources targeting overcoming TRAIL-resistance, activity-guided fractionation of the MeOH extract of Datura stramonium leaves led to the isolation of three alkaloids--scopolamine (1), trigonelline (2), and tyramine (3). Compounds 1, 2, and 3 exhibited TRAIL-resistance overcoming activity at 50, 150, and 100 µM, respectively in TRAIL-resistant AGS cells.
authors.forename: Utpal K
authors.initials: UK
authors.lastname: Karmakar
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Toume
authors.forename: Naoki
authors.initials: N
authors.lastname: Ishikawa
authors.forename: Midori A
authors.initials: MA
authors.lastname: Arai
authors.forename: Samir K
authors.initials: SK
authors.lastname: Sadhu
authors.forename: Firoj
authors.initials: F
authors.lastname: Ahmed
authors.forename: Masami
authors.initials: M
authors.lastname: Ishibashi
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D001681:Biological Assay / Q000379:methods*; D002460:Cell Line; D002470:Cell Survival; D013286:Datura stramonium / Q000737:chemistry*; D019008:Drug Resistance, Neoplasm; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000494:pharmacology*
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D053221:TNF-Related Apoptosis-Inducing Ligand