title: Russelioside B, a pregnane glycoside ameliorates hyperglycemia in streptozotocin induced diabetic rats by regulating key enzymes of glucose metabolism.
abstract: An alternative strategy to treat diabetes mellitus is the use of various natural agents possessing hypoglycemic effect. Caralluma quadrangula has been used in Saudi traditional medicine in cases of thirst and hunger and for the treatment of diabetes. The present study was designed to evaluate the improving effect of russelioside B, a pregnane glycoside isolated from Caralluma quadrangula on glucose metabolism in the liver of streptozotocin-induced diabetic rats. Diabetes mellitus was induced in rats by a single intraperitoneal injection of streptozotocin (50 mg/kg body weight). Experimental rats were administered russelioside B at a dose of 50 mg/kg body weight once a day for 30 days. The results showed that RB improved the fasting serum glucose level, glycated hemoglobin percent, serum insulin level and lipid profile. A significant improvement was observed upon the administration of russelioside B on the activities of the key enzymes of carbohydrate metabolism (glucokinase, glucose-6-phosphatase, glucose-6-phosphate dehydrogenase, and glycogen phosphorylase) in the liver of diabetic rats. Further, russelioside B administration to diabetic rats reverted gene expression of glucokinase, glucose-6-phosphatase, glycogen synthase and glycogen synthase kinase-3β to near normal levels. In conclusion, russelioside B possess antidiabetic and antihyperlipidemic effect in streptozotocin induced diabetic rats. Hence, administration of russelioside B may represent a potentially useful strategy for the management of diabetes.
authors.affiliation: Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt. Electronic address: abdelsattar@yahoo.com.
authors.forename: Essam
authors.initials: E
authors.lastname: Abdel-Sattar
authors.affiliation: Biochemistry Department, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt.
authors.forename: Shohda A
authors.initials: SA
authors.lastname: El-Maraghy
authors.affiliation: Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt.
authors.forename: Riham Salah
authors.initials: RS
authors.lastname: El-Dine
authors.affiliation: Biochemistry Department, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt.
authors.forename: Sherine M
authors.initials: SM
authors.lastname: Rizk
mesh_terms: D000818:Animals; D029062:Apocynaceae / Q000737:chemistry; D001786:Blood Glucose / Q000378:metabolism; D050260:Carbohydrate Metabolism / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000188:drug therapy* / Q000201:enzymology* / Q000378:metabolism; D005941:Glucokinase / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006442:Glycated Hemoglobin / Q000378:metabolism; D024981:Glycogen Phosphorylase / Q000378:metabolism; D006027:Glycosides / Q000737:chemistry / Q000627:therapeutic use*; D006943:Hyperglycemia / Q000150:complications / Q000188:drug therapy* / Q000201:enzymology* / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000627:therapeutic use*; D008297:Male; D011278:Pregnanes / Q000737:chemistry / Q000627:therapeutic use*; D017208:Rats, Wistar; D013311:Streptozocin
chemical_list: D001786:Blood Glucose; D005941:Glucokinase; D005947:Glucose; D005952:Glucose-6-Phosphatase; D005954:Glucosephosphate Dehydrogenase; D006442:Glycated Hemoglobin; D024981:Glycogen Phosphorylase; D006027:Glycosides; D007004:Hypoglycemic Agents; D011278:Pregnanes; D013311:Streptozocin; C574437:pregnane glycoside
keywords: Antihyperglycemic; Caralluma quadrangula; Carbohydrate metabolizing enzymes; Diabetes; Russelioside B