title: GENETIC MAPPING OF THE LAMINARIA JAPONICA (LAMINARALES, PHAEOPHYTA) USING AMPLIFIED FRAGMENT LENGTH POLYMORPHISM MARKERS(1).
abstract: To establish a molecular-marker-assisted system of breeding and genetic study for Laminaria japonica Aresch., amplified fragment length polymorphism (AFLP) was used to construct a genetic linkage map of L. japonica featuring 230 progeny of F2 cross population. Eighteen primer combinations produced 370 polymorphic loci and 215 polymorphic loci segregated in a 3:1 Mendelian segregation ratio (P ≤ 0.05). Of the 215 segregated loci, 142 were ordered into 27 linkage groups. The length of the linkage groups ranged from 6.7 to 90.3 centimorgans (cM) with an average length of 49.6 cM, and the total length was 1,085.8 cM, which covered 68.4% of the estimated 1,586.9 cM genome. The number of mapped markers on each linkage group ranged from 2 to 12, averaging 5.3 markers per group. The average density of the markers was 1 per 9.4 cM. Based on the marker density and the resolution of the map, the constructed linkage map can satisfy the need for quantitative trait locus (QTL) location and molecular-marker-assisted breeding for Laminaria.
authors.affiliation: Institute of Oceanology Chinese Academy of Sciences, Qingdao 266071, China and Graduate School of the Chinese Academy of Sciences, Beijing 100059, ChinaInstitute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaInstitute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China and Graduate School of the Chinese Academy of Sciences, Beijing 100059, ChinaInstitute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaLudong University, Yantai, 264025, China.
authors.forename: Fuli
authors.initials: F
authors.lastname: Liu
authors.affiliation: Institute of Oceanology Chinese Academy of Sciences, Qingdao 266071, China and Graduate School of the Chinese Academy of Sciences, Beijing 100059, ChinaInstitute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaInstitute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China and Graduate School of the Chinese Academy of Sciences, Beijing 100059, ChinaInstitute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaLudong University, Yantai, 264025, China.
authors.forename: Xiuliang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Oceanology Chinese Academy of Sciences, Qingdao 266071, China and Graduate School of the Chinese Academy of Sciences, Beijing 100059, ChinaInstitute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaInstitute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China and Graduate School of the Chinese Academy of Sciences, Beijing 100059, ChinaInstitute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaLudong University, Yantai, 264025, China.
authors.forename: Jidong
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Oceanology Chinese Academy of Sciences, Qingdao 266071, China and Graduate School of the Chinese Academy of Sciences, Beijing 100059, ChinaInstitute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaInstitute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China and Graduate School of the Chinese Academy of Sciences, Beijing 100059, ChinaInstitute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaLudong University, Yantai, 264025, China.
authors.forename: Wandong
authors.initials: W
authors.lastname: Fu
authors.affiliation: Institute of Oceanology Chinese Academy of Sciences, Qingdao 266071, China and Graduate School of the Chinese Academy of Sciences, Beijing 100059, ChinaInstitute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaInstitute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China and Graduate School of the Chinese Academy of Sciences, Beijing 100059, ChinaInstitute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaLudong University, Yantai, 264025, China.
authors.forename: Delin
authors.initials: D
authors.lastname: Duan
authors.affiliation: Institute of Oceanology Chinese Academy of Sciences, Qingdao 266071, China and Graduate School of the Chinese Academy of Sciences, Beijing 100059, ChinaInstitute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaInstitute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China and Graduate School of the Chinese Academy of Sciences, Beijing 100059, ChinaInstitute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaLudong University, Yantai, 264025, China.
authors.forename: Yingxia
authors.initials: Y
authors.lastname: Yang
keywords: Laminaria japonica; amplified fragment length polymorphism; genetic improvement; genetic linkage map; quantitative trait locus