title: Inhibition properties of propolis extracts to some clinically important enzymes.
abstract: The present study was conducted to envisage inhibition effects of propolis on the crucial enzymes, urease, xanthine oxidase (XO) and acetylcholinesterase (AChE). Some of the antioxidant properties of the propolis samples were determined using the total phenolic content (TPE) and total flavonoids in the eight different ethanolic propolis extracts (EPE) samples. Inhibition values of the enzymes were expressed as inhibition concentration (IC50; mg/mL or μg/mL) causing 50% inhibition of the enzymes with donepezil, acetohydroxamic acid and allopurinol as reference inhibitors. All the propolis extracts exhibited variable inhibition effects on these enzymes, but the higher the phenolic contents the lower the inhibitions values (IC50= 0.074 to 1.560 mg/mL). IC50values of the P5 propolis sample having the highest TPE, obtained from Zonguldak, for AChE, urease and XO were 0.081 ± 0.009, 0.080 ± 0.006 and 0.074 ± 0.011 μg/mL, respectively. The EPE proved to be a good source of inhibitor agents that can be used as natural inhibitors to serve human health.
authors.affiliation: a Department of Chemistry , Faculty of Arts & Science, Recep Tayyip Erdogan University , Rize , Turkey.
authors.forename: Nimet
authors.initials: N
authors.lastname: Baltas
authors.affiliation: b Maçka Vocational School, Karadeniz Thecnical University , Trabzon , Turkey , and.
authors.forename: Oktay
authors.initials: O
authors.lastname: Yildiz
authors.affiliation: c Department of Chemistry , Faculty of Science, Karadeniz Thecnical University , Trabzon , Turkey.
authors.forename: Sevgi
authors.initials: S
authors.lastname: Kolayli
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011429:Propolis / Q000737:chemistry*; D013329:Structure-Activity Relationship; D014510:Urease / Q000037:antagonists & inhibitors* / Q000378:metabolism; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D010936:Plant Extracts; D011429:Propolis; D014969:Xanthine Oxidase; D000110:Acetylcholinesterase; D014510:Urease
keywords: Acetylcholinesterase; enzyme inhibition; propolis; urease; xanthine oxidase