title: Alu methylation serves as a biomarker for non-invasive diagnosis of glioma.
abstract: Current techniques for diagnosing glioma are invasive and do not accurately predict prognosis. We developed a novel, non-invasive liquid chip assay to diagnose glioma and predict prognosis. Using this method, we determined the methylation state of the Alu element in cell-free DNA extracted from the serum of 109 glioma patients. Controls included 56 patients with benign intracranial tumors and 50 healthy subjects. Matched tumor tissues were processed for 36 patients. The cfDNA from glioma patients showed lower levels of Alu methylation than the controls (P<0.01). Alu methylation was also lower in high-grade than low-grade gliomas (P<0.01), indicating that Alu methylation correlates negatively with disease severity. Moreover, Alu methylation correlated positively with survival (P<0.01). These findings suggest high-throughput liquid chip could serve as a non-invasive diagnostic assay for glioma.
authors.affiliation: Department of Neurological Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.affiliation: Comprehensive Surgical Laboratory, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Huan
authors.affiliation: Comprehensive Surgical Laboratory, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zuo
authors.affiliation: Comprehensive Surgical Laboratory, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.
authors.forename: Longxiang
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Comprehensive Surgical Laboratory, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.
authors.forename: Chuanjun
authors.initials: C
authors.lastname: Huang
authors.affiliation: Comprehensive Surgical Laboratory, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.
authors.forename: Xiaojiang
authors.initials: X
authors.lastname: Liu
authors.affiliation: Comprehensive Surgical Laboratory, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.
authors.forename: Shiqiang
authors.initials: S
authors.lastname: Hou
authors.affiliation: Comprehensive Surgical Laboratory, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Qi
authors.affiliation: Department of Neurological Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.|Comprehensive Surgical Laboratory, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
mesh_terms: D020087:Alu Elements; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D001932:Brain Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D016022:Case-Control Studies; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000097:blood / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D005910:Glioma / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm
keywords: Alu; cell-free DNA; glioma; liquid chip; methylation