title: Single-Cell Lineage Tracing Reveals that Oriented Cell Division Contributes to Trabecular Morphogenesis and Regional Specification.
abstract: The cardiac trabeculae are sheet-like structures extending from the myocardium that function to increase surface area. A lack of trabeculation causes embryonic lethality due to compromised cardiac function. To understand the cellular and molecular mechanisms of trabecular formation, we genetically labeled individual cardiomyocytes prior to trabeculation via the brainbow multicolor system and traced and analyzed the labeled cells during trabeculation by whole-embryo clearing and imaging. The clones derived from labeled single cells displayed four different geometric patterns that are derived from different patterns of oriented cell division (OCD) and migration. Of the four types of clones, the inner, transmural, and mixed clones contributed to trabecular cardiomyocytes. Further studies showed that perpendicular OCD is an extrinsic asymmetric cell division that putatively contributes to trabecular regional specification. Furthermore, N-Cadherin deletion in labeled clones disrupted the clonal patterns. In summary, our data demonstrate that OCD contributes to trabecular morphogenesis and specification.
authors.affiliation: Center for Cardiovascular Sciences, Albany Medical College, Albany, NY 12208, USA.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
authors.affiliation: Center for Cardiovascular Sciences, Albany Medical College, Albany, NY 12208, USA.
authors.forename: Lianjie
authors.initials: L
authors.lastname: Miao
authors.affiliation: Center for Cardiovascular Sciences, Albany Medical College, Albany, NY 12208, USA.
authors.forename: David
authors.initials: D
authors.lastname: Shieh
authors.affiliation: Center for Cardiovascular Sciences, Albany Medical College, Albany, NY 12208, USA.
authors.forename: Ernest
authors.initials: E
authors.lastname: Spiotto
authors.affiliation: Key Laboratory of Molecular Medicine, Ministry of Education, Fudan University, Shanghai 200032, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Genetics, Albert Einstein College of Medicine, Yeshiva University, Bronx, NY 10461, USA.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Center for Cardiovascular Sciences, Albany Medical College, Albany, NY 12208, USA.
authors.forename: Antoni
authors.initials: A
authors.lastname: Paul
authors.affiliation: Biology and Biochemistry, University of Houston, Houston, TX 77204-5001, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Schwartz
authors.affiliation: Riley Heart Research Center, Indiana University, Indianapolis, IN 46202, USA.
authors.forename: Anthony B
authors.initials: AB
authors.lastname: Firulli
authors.affiliation: Center for Cardiovascular Sciences, Albany Medical College, Albany, NY 12208, USA.
authors.forename: Harold A
authors.initials: HA
authors.lastname: Singer
authors.affiliation: Key Laboratory of Molecular Medicine, Ministry of Education, Fudan University, Shanghai 200032, China.
authors.forename: Guoying
authors.initials: G
authors.lastname: Huang
authors.affiliation: Center for Cardiovascular Sciences, Albany Medical College, Albany, NY 12208, USA. Electronic address: wum@mail.amc.edu.
authors.forename: Mingfu
authors.initials: M
authors.lastname: Wu
mesh_terms: D000818:Animals; D060049:Asymmetric Cell Division; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D019070:Cell Lineage; D002465:Cell Movement; D005260:Female; D006321:Heart / Q000196:embryology*; D008297:Male; D051379:Mice; D009024:Morphogenesis; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism
chemical_list: D015820:Cadherins