title: Acute changes in liver tumour perfusion measured non-invasively with arterial spin labelling.
abstract: BACKGROUND
  Non-invasive measures of tumour vascular perfusion are desirable, in order to assess response to vascular targeting (or modifying) therapies. In this study, hepatic arterial spin labelling (ASL) magnetic resonance imaging (MRI) was investigated to measure acute changes in perfusion of colorectal cancer in the liver, in response to vascular disruption therapy with OXi4503.
  
  
  METHODS
  SW1222 and LS174T tumours were established in the liver of MF1 nu/nu mice via intrasplenic injection. Perfusion and R2(*) MRI measurements were acquired with an Agilent 9.4T horizontal bore scanner, before and at 90 min after 40 mg kg(-1) OXi4503.
  
  
  RESULTS
  A significant decrease in SW1222 tumour perfusion was observed (-43±33%, P<0.005). LS174T tumours had a significantly lower baseline level of perfusion. Intrinsic susceptibility MRI showed a significant increase in R2(*) in LS174T tumours (28±25%, P<0.05). An association was found between the change in tumour perfusion and the proximity to large vessels, with pre-treatment blood flow predictive of subsequent response. Histological evaluation confirmed the onset of necrosis and evidence of heterogeneous response between tumour deposits.
  
  
  CONCLUSIONS
  Hepatic ASL-MRI can detect acute response to targeted tumour vascular disruption entirely non-invasively. Hepatic ASL of liver tumours has potential for use in a clinical setting.
authors.affiliation: UCL Cancer Institute, University College London, Paul O'Gorman Building, 72 Huntley Street, London WC1E 6DD, UK.|UCL Centre for Advanced Biomedical Imaging, University College London, Paul O'Gorman Building, 72 Huntley Street, London WC1E 6DD, UK.
authors.forename: S Peter
authors.initials: SP
authors.lastname: Johnson
authors.affiliation: UCL Cancer Institute, University College London, Paul O'Gorman Building, 72 Huntley Street, London WC1E 6DD, UK.|UCL Centre for Advanced Biomedical Imaging, University College London, Paul O'Gorman Building, 72 Huntley Street, London WC1E 6DD, UK.
authors.forename: Rajiv
authors.initials: R
authors.lastname: Ramasawmy
authors.affiliation: UCL Centre for Advanced Biomedical Imaging, University College London, Paul O'Gorman Building, 72 Huntley Street, London WC1E 6DD, UK.|Cardiovascular and Pulmonary Branch, Division of Intramural Research, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Adrienne E
authors.initials: AE
authors.lastname: Campbell-Washburn
authors.affiliation: UCL Centre for Advanced Biomedical Imaging, University College London, Paul O'Gorman Building, 72 Huntley Street, London WC1E 6DD, UK.
authors.forename: Jack A
authors.initials: JA
authors.lastname: Wells
authors.affiliation: UCL Cancer Institute, University College London, Paul O'Gorman Building, 72 Huntley Street, London WC1E 6DD, UK.
authors.forename: Mathew
authors.initials: M
authors.lastname: Robson
authors.affiliation: UCL Cancer Institute, University College London, Paul O'Gorman Building, 72 Huntley Street, London WC1E 6DD, UK.
authors.forename: Vineeth
authors.initials: V
authors.lastname: Rajkumar
authors.affiliation: UCL Centre for Advanced Biomedical Imaging, University College London, Paul O'Gorman Building, 72 Huntley Street, London WC1E 6DD, UK.
authors.forename: Mark F
authors.initials: MF
authors.lastname: Lythgoe
authors.affiliation: UCL Cancer Institute, University College London, Paul O'Gorman Building, 72 Huntley Street, London WC1E 6DD, UK.
authors.forename: R Barbara
authors.initials: RB
authors.lastname: Pedley
authors.affiliation: UCL Centre for Advanced Biomedical Imaging, University College London, Paul O'Gorman Building, 72 Huntley Street, London WC1E 6DD, UK.
authors.forename: Simon
authors.initials: S
authors.lastname: Walker-Samuel
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000473:pathology; D005260:Female; D006499:Hepatic Artery / Q000473:pathology*; D008099:Liver / Q000473:pathology*; D008113:Liver Neoplasms / Q000175:diagnosis* / Q000473:pathology*; D018810:Magnetic Resonance Angiography / Q000379:methods; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D051379:Mice; D008819:Mice, Nude; D013113:Spin Labels
chemical_list: D013113:Spin Labels