title: Altered neutrophil immunophenotypes in childhood B‑cell precursor acute lymphoblastic leukemia.
abstract: An increasing number of evidences suggest a genetic predisposition in acute lymphoblastic leukemia (ALL) that might favor the occurrence of the driver genetic alterations. Such genetic background might also translate into phenotypic alterations of residual hematopoietic cells. Whether such phenotypic alterations are present in bone marrow (BM) cells from childhood B-cell precursor (BCP)-ALL remains to be investigated. Here we analyzed the immunophenotypic profile of BM and peripheral blood (PB) maturing/matured neutrophils from 118 children with BCP-ALL and their relationship with the features of the disease. Our results showed altered neutrophil phenotypes in most (77%) BCP-ALL cases. The most frequently altered marker was CD10 (53%), followed by CD33 (34%), CD13 (15%), CD15/CD65 (10%) and CD123 (7%). Of note, patients with altered neutrophil phenotypes had younger age (p = 0.03) and lower percentages of BM maturing neutrophils (p = 0.004) together with greater BM lymphocyte (p = 0.04), and mature B-cell (p = 0.03) counts. No significant association was found between an altered neutrophil phenotype and other disease features. These findings point out the potential existence of an altered residual hematopoiesis in most childhood BCP-ALL cases.
authors.affiliation: Clinical Medicine Postgraduate Program, College of Medicine, Rio de Janeiro Federal University (UFRJ), Rio de Janeiro, Brazil.|Cytometry Service, Institute of Pediatrics and Puericulture Martagão Gesteira (IPPMG), UFRJ, Rio de Janeiro, Brazil.
authors.forename: Elen
authors.initials: E
authors.lastname: Oliveira
authors.affiliation: Cytometry Service, Institute of Pediatrics and Puericulture Martagão Gesteira (IPPMG), UFRJ, Rio de Janeiro, Brazil.
authors.forename: Thiago S
authors.initials: TS
authors.lastname: Bacelar
authors.affiliation: Departament of Medicine and Cytometry Service, Cancer Research Center (IBMCC, USAL-CSIC), Institute for Biomedical Research of Salamanca (IBSAL), University of Salamanca (USAL), Salamanca, Spain.
authors.forename: Juana
authors.initials: J
authors.lastname: Ciudad
authors.affiliation: Cytogenetics Service, IPPMG-UFRJ and Polo Xerém-UFRJ, Rio de Janeiro, Brazil.
authors.forename: Maria Cecília M
authors.initials: MC
authors.lastname: Ribeiro
authors.affiliation: Clinical Medicine Postgraduate Program, College of Medicine, Rio de Janeiro Federal University (UFRJ), Rio de Janeiro, Brazil.|Cytogenetics Department, Bone Marrow Transplantation Unit and Oncology Post Graduation Program, National Cancer Institute (INCa), Rio de Janeiro, Brazil.
authors.forename: Daniela R N
authors.initials: DR
authors.lastname: Garcia
authors.affiliation: Department of Pediatric Hematology/Oncology, Medical University of Silesia, Zabrze, Poland.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Sedek
authors.affiliation: Service of Pediatric Hematology, Federal Lagoa Hospital (HFL), Rio de Janeiro, Brazil.
authors.forename: Simone F
authors.initials: SF
authors.lastname: Maia
authors.affiliation: Clinical Medicine Postgraduate Program, College of Medicine, Rio de Janeiro Federal University (UFRJ), Rio de Janeiro, Brazil.
authors.forename: Daniel B
authors.initials: DB
authors.lastname: Aranha
authors.affiliation: Service of Pediatric Hematology, São José do Avaí Hospital (HSJA), Itaperuna, Rio de Janeiro, Brazil.
authors.forename: Indyara C
authors.initials: IC
authors.lastname: Machado
authors.affiliation: Service of Pediatric Hematology/Oncology, Servidores do Estado Federal Hospital (HSE), Rio de Janeiro, Brazil.
authors.forename: Arissa
authors.initials: A
authors.lastname: Ikeda
authors.affiliation: Service of Pediatric Hematology, Children's Cancer Hospital of Barretos, Barretos, São Paulo, Brazil.
authors.forename: Bianca F
authors.initials: BF
authors.lastname: Baglioli
authors.affiliation: Cytometry Service, Institute of Pediatrics and Puericulture Martagão Gesteira (IPPMG), UFRJ, Rio de Janeiro, Brazil.
authors.forename: Nathalia
authors.initials: N
authors.lastname: Lopez-Duarte
authors.affiliation: Clinical Medicine Postgraduate Program, College of Medicine, Rio de Janeiro Federal University (UFRJ), Rio de Janeiro, Brazil.|Cytometry Service, Institute of Pediatrics and Puericulture Martagão Gesteira (IPPMG), UFRJ, Rio de Janeiro, Brazil.
authors.forename: Lisandra A C
authors.initials: LA
authors.lastname: Teixeira
authors.affiliation: Department of Pediatric Hematology/Oncology, Medical University of Silesia, Zabrze, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Szczepanski
authors.affiliation: Cytogenetics Department, Bone Marrow Transplantation Unit and Oncology Post Graduation Program, National Cancer Institute (INCa), Rio de Janeiro, Brazil.
authors.forename: Maria Luiza M
authors.initials: ML
authors.lastname: Silva
authors.affiliation: Clinical Medicine Postgraduate Program, College of Medicine, Rio de Janeiro Federal University (UFRJ), Rio de Janeiro, Brazil.|Cytometry Service, Institute of Pediatrics and Puericulture Martagão Gesteira (IPPMG), UFRJ, Rio de Janeiro, Brazil.
authors.forename: Marcelo G P
authors.initials: MG
authors.lastname: Land
authors.affiliation: Departament of Medicine and Cytometry Service, Cancer Research Center (IBMCC, USAL-CSIC), Institute for Biomedical Research of Salamanca (IBSAL), University of Salamanca (USAL), Salamanca, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Orfao
authors.affiliation: Clinical Medicine Postgraduate Program, College of Medicine, Rio de Janeiro Federal University (UFRJ), Rio de Janeiro, Brazil.|Cytometry Service, Institute of Pediatrics and Puericulture Martagão Gesteira (IPPMG), UFRJ, Rio de Janeiro, Brazil.
authors.forename: Elaine S
authors.initials: ES
authors.lastname: Costa
mesh_terms: D002648:Child; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D009504:Neutrophils / Q000276:immunology*; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology*
keywords: B-cell precursor acute lymphoblastic leukemia; altered neutrophil immunophenotype; childhood; multiparameter flow cytometry; residual hematopoiesis