title: [Expression of FAS-AS1 in breast cancer and its effect on the host gene sFas].
abstract: OBJECTIVE
  To investigate the expression level of antisense transcript of FAS (FAS-antisense 1, FAS-AS1) in breast cancer, and its effect on the host gene soluble Fas receptor (sFas).
  
  
  METHODS
  The expression of FAS-AS1 and sFas gene was detected in 20 cases of breast cancer tumors and breast cancer cells and their normal counterparts by using real-time quantitative polymerase chain reaction(RT-qPCR). The effects of FAS-AS1 on the expression of sFas gene in breast cancer cells were analyzed by overexpressing FAS-AS1 in breast cancer cells.
  
  
  RESULTS
  FAS-AS1 expression in breast cancer tissue and cell lines was significantly lower than that of adjacent breast tissue (P=0.000) and normal breast cells (P<0.01). However, sFasexpression in the breast cancer tissue and cell lines was significantly higher than that of adjacent breast tissue (P=0.001) and normal breast cells (P<0.01). The expression of FAS-AS1 was negatively correlated with the expression of sFas(r=-0.751, P=0.020). FAS-AS1 expression was observed to be closely correlated with tumor size (r(2)=0.351, P=0.025) and lymph node metastasis (r(2)=0.265, P=0.043). Expression of sFas and cell proliferation was significantly reduced in FAS-AS1-overexpressing MCF-7 cells (P<0.05).
  
  
  CONCLUSIONS
  FAS-AS1 is distinctly downexpressed in breast cancer, and it has certain effects on the expression of host gene sFas and breast cancer development.
authors.affiliation: Department of Head & Neck, and Breast Surgery, Xinxiang Central Hospital, Xinxiang, Henan 453000, China.
authors.forename: S W
authors.initials: SW
authors.lastname: Zhou
authors.forename: B B
authors.initials: BB
authors.lastname: Su
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D001943:Breast Neoplasms / Q000097:blood*; D053222:Fas Ligand Protein / Q000235:genetics* / Q000378:metabolism; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D060888:Real-Time Polymerase Chain Reaction; D019014:fas Receptor / Q000235:genetics* / Q000378:metabolism
chemical_list: C491901:FAS protein, human; D053222:Fas Ligand Protein; D019014:fas Receptor