title: Light induces translocation of NF-κB p65 to the mitochondria and suppresses expression of cytochrome c oxidase subunit III (COX III) in the rat retina.
abstract: The transcription factor nuclear factor kappaB (NF-κB) plays various roles in cell survival, apoptosis, and inflammation. In the rat retina, NF-κB activity increases after exposure to damaging light, resulting in degeneration of photoreceptors. Here, we report that in dark-adapted rats exposed for 6 h to bright white light, the p65 subunit of retinal NF-κB translocates to the mitochondria, an event associated with a decrease in expression of cytochrome c oxidase subunit III (COX III). However, sustained exposure for 12 h depleted p65 from the mitochondria, and enhanced COX III expression. Treatment with the protective antioxidant PBN prior to light exposure prevents p65 depletion in the mitochondria and COX III upregulation during prolonged exposure, and apoptosis in photoreceptor cells. These results indicate that COX III expression is sensitive to the abundance of NF-κB p65 in the mitochondria, which, in turn, is affected by exposure to damaging light.
authors.affiliation: Laboratory of Visual Neuroscience, Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering, 4-3-5 Ueda, Morioka, Iwate 020-8551, Japan; Soft-Path Engineering Research Center (SPERC), Faculty of Science and Engineering, Iwate University, Morioka 020-8551, Japan; Clinical Research, Innovation and Education Center, Tohoku University Hospital, 1-1 Seiryo, Aoba, Sendai, Miyagi 980-8574, Japan. Electronic address: htomita@iwate-u.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tomita
authors.affiliation: Laboratory of Visual Neuroscience, Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering, 4-3-5 Ueda, Morioka, Iwate 020-8551, Japan. Electronic address: ktabata@iwate-u.ac.jp.
authors.forename: Kitako
authors.initials: K
authors.lastname: Tabata
authors.affiliation: Laboratory of Visual Neuroscience, Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering, 4-3-5 Ueda, Morioka, Iwate 020-8551, Japan. Electronic address: mqdelta@iwate-u.ac.jp.
authors.forename: Maki
authors.initials: M
authors.lastname: Takahashi
authors.affiliation: Laboratory of Visual Neuroscience, Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering, 4-3-5 Ueda, Morioka, Iwate 020-8551, Japan. Electronic address: t2114018@iwate-u.ac.jp.
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Nishiyama
authors.affiliation: Laboratory of Visual Neuroscience, Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering, 4-3-5 Ueda, Morioka, Iwate 020-8551, Japan; Soft-Path Engineering Research Center (SPERC), Faculty of Science and Engineering, Iwate University, Morioka 020-8551, Japan. Electronic address: sseriko@iwate-u.ac.jp.
authors.forename: Eriko
authors.initials: E
authors.lastname: Sugano
mesh_terms: D000818:Animals; D017209:Apoptosis; D003497:Cyclic N-Oxides / Q000494:pharmacology; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000528:radiation effects*; D020672:I-kappa B Proteins / Q000378:metabolism; D008027:Light; D008928:Mitochondria / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D021381:Protein Transport / Q000528:radiation effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D051996:Transcription Factor RelA / Q000378:metabolism*
chemical_list: D003497:Cyclic N-Oxides; D020672:I-kappa B Proteins; D016328:NF-kappa B; C498145:Rela protein, rat; D051996:Transcription Factor RelA; C029217:phenyl-N-tert-butylnitrone; D003576:Electron Transport Complex IV
keywords: Cytochrome C oxidase III; Light damage; NF-κB; Retina