title: The Regulatory Roles of ncRNA Rli60 in Adaptability of Listeria monocytogenes to Environmental Stress and Biofilm Formation.
abstract: Listeria monocytogenes is a facultative anaerobic Gram-positive bacterium. It is well adapted to external environments and able to infect both humans and animals. To understand the impacts of ncRNA Rli60 on the adaptability of L. monocytogenes to environmental stresses and biofilm formation, a rli60 deletion strain of L. monocytogenes (LM-Δrli60) was constructed using splicing by overlap extension PCR (SOE-PCR) and homologous recombination and then compared it with wild-type strain L. monocytogenes EGD-e in the aspects of adaptability to environmental stresses by measuring their growth under stresses of different temperatures, and acidic, alkaline, hypertonic and alcoholic conditions, and capability of biofilm formation by using crystal violet staining, as well as the transcriptional levels of genes (gltB and gltC) related to the biofilm formation by real-time quantitative PCR (qRT-PCR). The results showed that (1) the growth of LM-Δrli60 strain was significantly slower under environmental stresses of low temperature (30 °C), high temperature (42 °C), as well as alkaline and alcoholic conditions, (2) the amount of biofilm formed by LM-Δrli60 was attenuated, and (3) the transcriptional levels of gltB and gltC genes at 24 h and 48 h in LM-Δrli60 revealed a significant reduction. Overall, the results confirmed that ncRNA Rli60 plays important roles in regulating the adaptability of L. monocytogenes to environmental stresses and biofilm formation possibly through impacting the expression of gltB and gltC genes.
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
authors.forename: Ye-Long
authors.initials: YL
authors.lastname: Peng
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
authors.forename: Qing-Ling
authors.initials: QL
authors.lastname: Meng
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China. qj710625@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Qiao
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Xie
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Chen
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
authors.forename: Tian-Li
authors.initials: TL
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
authors.forename: Zheng-Xiang
authors.initials: ZX
authors.lastname: Hu
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Ma
authors.affiliation: State Key Lab of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China.
authors.forename: Xue-Peng
authors.initials: XP
authors.lastname: Cai
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
authors.forename: Chuang-Fu
authors.initials: CF
authors.lastname: Chen
mesh_terms: D000222:Adaptation, Physiological; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018441:Biofilms; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D008089:Listeria monocytogenes / Q000235:genetics / Q000502:physiology*; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D001426:Bacterial Proteins; D012329:RNA, Bacterial; D062085:RNA, Long Noncoding