title: Discovery of piRNAs Pathway Associated with Early-Stage Spermatogenesis in Chicken.
abstract: Piwi-interacting RNAs (piRNAs) play a key role in spermatogenesis. Here, we describe the piRNAs profiling of primordial germ cells (PGCs), spermatogonial stem cells (SSCs), and the spermatogonium (Sp) during early-stage spermatogenesis in chicken. We obtained 31,361,989 reads from PGCs, 31,757,666 reads from SSCs, and 46,448,327 reads from Sp cells. The length distribution of piRNAs in the three samples showed peaks at 33 nt. The resulting genes were subsequently annotated against the Gene Ontology (GO) database. Five genes (RPL7A, HSPA8, Pum1, CPXM2, and PRKCA) were found to be involved in cellular processes. Interactive pathway analysis (IPA) further revealed three important pathways in early-stage spermatogenesis including the FGF, Wnt, and EGF receptor signaling pathways. The gene Pum1 was found to promote germline stem cell proliferation, but it also plays a role in spermatogenesis. In conclusion, we revealed characteristics of piRNAs during early spermatogonial development in chicken and provided the basis for future research.
authors.affiliation: College of Animal Science & Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Xu
authors.affiliation: College of Animal Science & Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Qiu
authors.affiliation: College of Animal Science & Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
authors.forename: Guobin
authors.initials: G
authors.lastname: Chang
authors.affiliation: College of Animal Science & Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
authors.forename: Qixin
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, Jiangsu, 225003, China.
authors.forename: Xiangping
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Animal Science & Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Bi
authors.affiliation: College of Animal Science & Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, Jiangsu, 225003, China.
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Animal Science & Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
authors.forename: Zhiteng
authors.initials: Z
authors.lastname: Li
authors.affiliation: College of Animal Science & Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Guo
authors.affiliation: College of Animal Science & Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wan
authors.affiliation: College of Animal Science & Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science & Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Xu
authors.affiliation: College of Animal Science & Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
authors.forename: Guohong
authors.initials: G
authors.lastname: Chen
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D030161:Avian Proteins / Q000096:biosynthesis*; D049109:Cell Proliferation / Q000502:physiology; D002645:Chickens / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D034741:RNA, Small Interfering / Q000378:metabolism*; D013091:Spermatogenesis / Q000502:physiology*
chemical_list: D030161:Avian Proteins; D034741:RNA, Small Interfering