title: Evaluation of lateral flow assay as a field test for investigation of brucellosis outbreak in an organized buffalo farm: A pilot study.
abstract: OBJECTIVE
  The aim was to evaluate lateral flow assay (LFA) as a field test for investigation of brucellosis outbreak in organized buffalo farm.
  
  
  METHODS
  A total of 153 serum samples were tested to detect the presence of brucella antibodies by LFA and three other serological tests i.e. rose bengal plate test (RBPT), protein G based indirect enzyme-linked immunoassay (iELISA), and competitive ELISA (cELISA). The performances of LFA and other serological tests were evaluated using OIE complaint cELISA as the gold standard.
  
  
  RESULTS
  Serological tests revealed 50% of the animals were seropositive for Brucella antibodies and correlated with clinical history of abortions, infertility, and productive failures. The newly developed assay showed 87.1% and 92.6% sensitivity and specificity, which was even higher than the specificity of RBPT.
  
  
  CONCLUSIONS
  The investigation proved the potential usefulness of LFA for field diagnosis of brucellosis in the regions where laboratory facilities are limited.
authors.affiliation: Bacteriology Lab-1, National Institute of Veterinary Epidemiology and Disease Informatics (Formerly PD_ADMAS), Ramagondanahalli, Yelahanka, Bengaluru - 560 064, Karnataka, India.
authors.forename: R
authors.initials: R
authors.lastname: Shome
authors.affiliation: Animal Disease Research Centre, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana - 141 004, Punjab, India.
authors.forename: G
authors.initials: G
authors.lastname: Filia
authors.affiliation: Bacteriology Lab-1, National Institute of Veterinary Epidemiology and Disease Informatics (Formerly PD_ADMAS), Ramagondanahalli, Yelahanka, Bengaluru - 560 064, Karnataka, India.
authors.forename: B S
authors.initials: BS
authors.lastname: Padmashree
authors.affiliation: Bacteriology Lab-1, National Institute of Veterinary Epidemiology and Disease Informatics (Formerly PD_ADMAS), Ramagondanahalli, Yelahanka, Bengaluru - 560 064, Karnataka, India.
authors.forename: N
authors.initials: N
authors.lastname: Krithiga
authors.affiliation: Bacteriology Lab-1, National Institute of Veterinary Epidemiology and Disease Informatics (Formerly PD_ADMAS), Ramagondanahalli, Yelahanka, Bengaluru - 560 064, Karnataka, India.
authors.forename: Swati
authors.initials: S
authors.lastname: Sahay
authors.affiliation: Bacteriology Lab-1, National Institute of Veterinary Epidemiology and Disease Informatics (Formerly PD_ADMAS), Ramagondanahalli, Yelahanka, Bengaluru - 560 064, Karnataka, India.
authors.forename: K
authors.initials: K
authors.lastname: Triveni
authors.affiliation: Bacteriology Lab-1, National Institute of Veterinary Epidemiology and Disease Informatics (Formerly PD_ADMAS), Ramagondanahalli, Yelahanka, Bengaluru - 560 064, Karnataka, India.
authors.forename: B R
authors.initials: BR
authors.lastname: Shome
authors.affiliation: Animal Disease Research Centre, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana - 141 004, Punjab, India.
authors.forename: V
authors.initials: V
authors.lastname: Mahajan
authors.affiliation: Animal Disease Research Centre, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana - 141 004, Punjab, India.
authors.forename: Amarjit
authors.initials: A
authors.lastname: Singh
authors.affiliation: Bacteriology Lab-1, National Institute of Veterinary Epidemiology and Disease Informatics (Formerly PD_ADMAS), Ramagondanahalli, Yelahanka, Bengaluru - 560 064, Karnataka, India.
authors.forename: H
authors.initials: H
authors.lastname: Rahman
keywords: brucellosis outbreak; competitive enzyme-linked immunoassay; indirect enzyme-linked immunoassay; lateral flow assay; rose bengal plate test