title: Determination of β-glucuronidase in human colorectal carcinoma cell lines.
abstract: OBJECTIVE
  To study the relationship between β-glucuronidase and the invasiveness of human colorectal carcinoma cell lines.
  
  
  METHODS
  Six colorectal carcinoma cell lines, including three well-differentiated (CX1, CCL187, and CCL229) and three poorly differentiated ones (CCL227, CCL228, and Clone A), were analyzed by Fischman's method to determine the concentration of β-glucuronidase in the medium.
  
  
  RESULTS
  Low levels of β-glucuronidase (activity range: 1.29 to 1.96 μg/106 cells·h) were associated with poor invasiveness. This finding was in contrast to the elevated levels of the enzyme (2.46-3.37 μg/106·h) detected in the medium derived from the more aggressively invasive cells (CCL 227, CCL 228, Clone A, and CCL 229).
  
  
  CONCLUSIONS
  Highly invasive colorectal carcinoma cells secreted higher levels of β-glucuronidase than the poorly invasive cells. Determination of secreted β-glucuronidase might represent a useful in vitro measurement tool to assess the invasiveness of colorectal carcinoma.
authors.affiliation: Shu Feng, Jin-Dan Song, Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, Shenyang 110001, Liaonin Province, China.
authors.forename: S
authors.initials: S
authors.lastname: Feng
authors.affiliation: Shu Feng, Jin-Dan Song, Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, Shenyang 110001, Liaonin Province, China.
authors.forename: J D
authors.initials: JD
authors.lastname: Song
keywords: Cell lines; Colorectal neoplasms; β-glucuronidase invasiveness