title: Comparison of Predictive Immunohistochemical Marker Expression of Primary Breast Cancer and Paired Distant Metastasis using Surgical Material: A Practice-Based Study.
abstract: Parallel studies of primary breast carcinomas and corresponding distant metastases samples reveal considerable differences. Our aim was to highlight this issue from another perspective and provide further data based on 98 patient samples: 69 primary breast carcinoma and 85 distant metastases from bone, central nervous system (CNS) and lung (56 paired). Two independent series of immunohistochemical reactions with different antibodies for estrogen receptor (ER), progesterone receptor (PgR) and human epidermal growth factor receptor 2 (Her2), along with HER2 fluroscence in situ hybridization (FISH) were performed on tissue microarrays to classify breast carcinoma and distant metastases samples into Luminal A, Luminal B-proliferating, Luminal B-HER2+, HER2+ and triple negative (TNBC) surrogate breast cancer groups. Correlation and agreement between the two assessments of ER and PgR were fair-to-moderate, and almost perfect for HER2 and Ki67. There was 40% discordance concerning immunophenotype between breast carcinomas and distant metastases. Most common metastatic site of ER+ breast carcinoma was the skeletal system (59.2%), whereas that of TNBCs was the CNS (58.8%) and lungs (23.5%). Distant metastases in bones were mostly luminal (54.3%), in the CNS, Luminal B (53.2%), and in the lung, TNBC (37.5%). The change of drugable properties of primary breast cancers in the respective bone and CNS metastases suggests that characterization of the metastasis is necessary for appropriate treatment planning.
authors.affiliation: 2nd Department of Pathology, Semmelweis University, Hungary (JK, BS, LVL, OK, AMT, AMS)
authors.forename: Janina
authors.initials: J
authors.lastname: Kulka
authors.affiliation: 2nd Department of Pathology, Semmelweis University, Hungary (JK, BS, LVL, OK, AMT, AMS)
authors.forename: Borbála
authors.initials: B
authors.lastname: Székely
authors.affiliation: 2nd Department of Pathology, Semmelweis University, Hungary (JK, BS, LVL, OK, AMT, AMS)
authors.forename: Lilla V
authors.initials: LV
authors.lastname: Lukács
authors.affiliation: 2nd Department of Pathology, Semmelweis University, Hungary (JK, BS, LVL, OK, AMT, AMS)
authors.forename: Orsolya
authors.initials: O
authors.lastname: Kiss
authors.affiliation: 2nd Department of Pathology, Semmelweis University, Hungary (JK, BS, LVL, OK, AMT, AMS)|Hungarian Academy of Sciences, MTA-SE Tumour Progression Research Group, 2nd Department of Pathology, Semmelweis University, Hungary (AMT)
authors.forename: Anna-Mária
authors.initials: AM
authors.lastname: Tőkés
authors.affiliation: 1st Department of Pathology and Experimental Cancer Research, Semmelweis University, Hungary (EV, ET)
authors.forename: Eszter
authors.initials: E
authors.lastname: Vincze
authors.affiliation: 1st Department of Pathology and Experimental Cancer Research, Semmelweis University, Hungary (EV, ET)
authors.forename: Eszter
authors.initials: E
authors.lastname: Turányi
authors.affiliation: Department of Pathology, Korányi National Institute for Tuberculosis and Pulmonology, Hungary (JF)
authors.forename: János
authors.initials: J
authors.lastname: Fillinger
authors.affiliation: Department of Pathology, National Institute of Neurosurgery, Hungary (ZH)
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Hanzély
authors.affiliation: Department of Orthopedics, Semmelweis University, Hungary (GA, MS)
authors.forename: Gabriella
authors.initials: G
authors.lastname: Arató
authors.affiliation: Department of Orthopedics, Semmelweis University, Hungary (GA, MS)
authors.forename: Miklós
authors.initials: M
authors.lastname: Szendrői
authors.affiliation: MTA-TTK Lendület Cancer Biomarker Research Group, Hungarian Academy of Sciences, Hungary (BG, AMS)|2nd Department of Pediatrics, Semmelweis University Budapest, Hungary (BG).
authors.forename: Balázs
authors.initials: B
authors.lastname: Győrffy
authors.affiliation: 2nd Department of Pathology, Semmelweis University, Hungary (JK, BS, LVL, OK, AMT, AMS)|MTA-TTK Lendület Cancer Biomarker Research Group, Hungarian Academy of Sciences, Hungary (BG, AMS)
authors.forename: A Marcell
authors.initials: AM
authors.lastname: Szász
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis; D001859:Bone Neoplasms / Q000175:diagnosis / Q000473:pathology / Q000556:secondary*; D001842:Bone and Bones / Q000473:pathology; D001940:Breast / Q000473:pathology*; D001943:Breast Neoplasms / Q000175:diagnosis / Q000473:pathology*; D002490:Central Nervous System / Q000473:pathology; D016543:Central Nervous System Neoplasms / Q000175:diagnosis / Q000473:pathology / Q000556:secondary*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis; D008175:Lung Neoplasms / Q000175:diagnosis / Q000473:pathology / Q000556:secondary*; D011379:Prognosis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis; D011960:Receptors, Estrogen / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; C508053:ERBB2 protein, human
keywords: bone; breast cancer; central nervous system; immunoprofiling; lung; metastasis