title: Exploiting Secreted Luciferases to Monitor Tumor Progression In Vivo.
abstract: Secreted luciferases provide a simple, accurate, and cost-effective tool to monitor tumor response after treatment in small animal models. This protocol describes all the steps required to generate human tumor cell lines expressing this reporter gene and to monitor in vivo tumor progression after injection in immunocompromised mice by means of serial peripheral blood sampling and analysis.
authors.affiliation: Innovative Immunotherapies Unit, IRCCS San Raffaele Hospital, Via Olgettina 60, 21032, Milano, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Falcone
authors.affiliation: Innovative Immunotherapies Unit, IRCCS San Raffaele Hospital Scientific Institute, Via Olgettina 60, 21032, Milano, Italy. casucci.monica@hsr.it.
authors.forename: Monica
authors.initials: M
authors.lastname: Casucci
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D058948:Cell Tracking; D018450:Disease Progression; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology; D047368:Tumor Burden
chemical_list: D008156:Luciferases
keywords: Animal models; Cancer therapy; In vivo imaging; Monitoring tumor growth in vivo; Secreted luciferases