title: Proton mediated control of biochemical reactions with bioelectronic pH modulation.
abstract: In Nature, protons (H(+)) can mediate metabolic process through enzymatic reactions. Examples include glucose oxidation with glucose dehydrogenase to regulate blood glucose level, alcohol dissolution into carboxylic acid through alcohol dehydrogenase, and voltage-regulated H(+) channels activating bioluminescence in firefly and jellyfish. Artificial devices that control H(+) currents and H(+) concentration (pH) are able to actively influence biochemical processes. Here, we demonstrate a biotransducer that monitors and actively regulates pH-responsive enzymatic reactions by monitoring and controlling the flow of H(+) between PdHx contacts and solution. The present transducer records bistable pH modulation from an "enzymatic flip-flop" circuit that comprises glucose dehydrogenase and alcohol dehydrogenase. The transducer also controls bioluminescence from firefly luciferase by affecting solution pH.
authors.affiliation: Department of Materials Science and Engineering, University of Washington, Seattle, WA 98195-2120, USA.
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of Materials Science and Engineering, University of Washington, Seattle, WA 98195-2120, USA.|Department of Electrical Engineering, Jack Baskin School of Engineering, University of California, Santa Cruz, CA 95064, USA.
authors.forename: Takeo
authors.initials: T
authors.lastname: Miyake
authors.affiliation: Department of Materials Science and Engineering, University of Washington, Seattle, WA 98195-2120, USA.
authors.forename: Scott
authors.initials: S
authors.lastname: Keene
authors.affiliation: Department of Materials Science and Engineering, University of Washington, Seattle, WA 98195-2120, USA.|Department of Electrical Engineering, University of Washington, Seattle, WA 98195-2500, USA.
authors.forename: Erik E
authors.initials: EE
authors.lastname: Josberger
authors.affiliation: Department of Materials Science and Engineering, University of Washington, Seattle, WA 98195-2120, USA.|Department of Electrical Engineering, Jack Baskin School of Engineering, University of California, Santa Cruz, CA 95064, USA.
authors.forename: Marco
authors.initials: M
authors.lastname: Rolandi
mesh_terms: D001669:Biochemical Phenomena; D004581:Electronics / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements; D011522:Protons; D012815:Signal Processing, Computer-Assisted; D012996:Solutions; D014159:Transducers
chemical_list: D011522:Protons; D012996:Solutions