title: Cloning, expression, purification and crystallization of Schizosaccharomyces pombe Set7, a putative histone methyltransferase.
abstract: Dysfunction of histone-modifying enzymes affects chromatin regulation and is involved in carcinogenesis, tumour progression and other diseases. Histone methyltransferases are a family of key histone-modifying enzymes, but their structures, functions and mechanisms are incompletely understood, thus constraining drug-design efforts. Here, preliminary steps towards structure-function studies of Schizosaccharomyces pombe Set7, a putative histone methyltransferase and the first yeast full-length SET-domain-containing protein to be studied using X-ray crystallography, are reported. The methods from cloning to X-ray diffraction and phasing are discussed and the results will aid in prospective studies of histone-modifying enzymes.
authors.affiliation: Genetic Engineering Department, School of Life Sciences, College of Natural Sciences, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu 41566, Republic of Korea.
authors.forename: Damiaan E H F
authors.initials: DE
authors.lastname: Mevius
authors.affiliation: Genetic Engineering Department, School of Life Sciences, College of Natural Sciences, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu 41566, Republic of Korea.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Genetic Engineering Department, School of Life Sciences, College of Natural Sciences, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu 41566, Republic of Korea.
authors.forename: Masayo
authors.initials: M
authors.lastname: Morishita
authors.affiliation: Genetic Engineering Department, School of Life Sciences, College of Natural Sciences, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu 41566, Republic of Korea.
authors.forename: Eric
authors.initials: E
authors.lastname: di Luccio
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003460:Crystallization; D000076983:Histone Methyltransferases; D011495:Histone-Lysine N-Methyltransferase; D012568:Schizosaccharomyces / Q000737:chemistry*; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D029702:Schizosaccharomyces pombe Proteins; D000076983:Histone Methyltransferases; D011495:Histone-Lysine N-Methyltransferase
keywords: Schizosaccharomyces pombe; Set7; X-ray crystallography; histone methyltransferase; histone modifications