title: Evaluation of specific humoral immune response in pigs vaccinated with cell culture adapted classical swine fever vaccine.
abstract: OBJECTIVE
  To determine an efficient vaccination schedule on the basis of the humoral immune response of cell culture adapted live classical swine fever virus (CSFV) vaccinated pigs and maternally derived antibody (MDA) in piglets of vaccinated sows.
  
  
  METHODS
  A cell culture adapted live CSFV vaccine was subjected to different vaccination schedule in the present study. Serum samples were collected before vaccination (day 0) and 7, 14, 28, 42, 56, 180, 194, 208, 270, 284 and 298 days after vaccination and were analyzed by liquid phase blocking enzyme-linked immunosorbent assay. Moreover, MDA titre was detected in the serum of piglets at 21 and 42 days of age after farrowing of the vaccinated sows.
  
  
  RESULTS
  On 28 days after vaccination, serum samples of 83.33% vaccinated pigs showed the desirable level of antibody titer (log10 1.50 at 1:32 dilution), whereas 100% animals showed log10 1.50 at 1:32 dilution after 42 days of vaccination. Animals received a booster dose at 28 and 180 days post vaccination showed stable high-level antibody titre till the end of the study period. Further, piglets born from pigs vaccinated 1 month after conception showed the desirable level of MDA up to 42 days of age.
  
  
  CONCLUSIONS
  CSF causes major losses in pig industry. Lapinised vaccines against CSFV are used routinely in endemic countries. In the present study, a cell culture adapted live attenuated vaccine has been evaluated. Based on the level of humoral immune response of vaccinated pigs and MDA titer in piglets born from immunized sows, it may be concluded that the more effective vaccination schedule for prevention of CSF is primary vaccination at 2 months of age followed by booster vaccination at 28 and 180 days post primary vaccination and at 1 month of gestation.
authors.affiliation: Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India.
authors.forename: Mrinal K
authors.initials: MK
authors.lastname: Nath
authors.affiliation: ICAR-National Research Centre on Pig, Rani, Guwahati, Assam, India.
authors.forename: D K
authors.initials: DK
authors.lastname: Sarma
authors.affiliation: Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India.
authors.forename: B C
authors.initials: BC
authors.lastname: Das
authors.affiliation: Department of Veterinary Microbiology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India.
authors.forename: P
authors.initials: P
authors.lastname: Deka
authors.affiliation: ICAR - All India Co-ordinated Research Project on Pigs, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India.
authors.forename: D
authors.initials: D
authors.lastname: Kalita
authors.affiliation: Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India.
authors.forename: J B
authors.initials: JB
authors.lastname: Dutta
authors.affiliation: Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India.
authors.forename: G
authors.initials: G
authors.lastname: Mahato
authors.affiliation: Department of Veterinary Bio-chemistry, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India.
authors.forename: S
authors.initials: S
authors.lastname: Sarma
authors.affiliation: Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Central Agricultural University, Aizawal, Mizoram, India.
authors.forename: P
authors.initials: P
authors.lastname: Roychoudhury
keywords: antibody titer; classical swine fever vaccine; liquid phase blocking-enzyme-linked immunosorbent assay; pig