title: Folding complex DNA nanostructures from limited sets of reusable sequences.
abstract: Scalable production of DNA nanostructures remains a substantial obstacle to realizing new applications of DNA nanotechnology. Typical DNA nanostructures comprise hundreds of DNA oligonucleotide strands, where each unique strand requires a separate synthesis step. New design methods that reduce the strand count for a given shape while maintaining overall size and complexity would be highly beneficial for efficiently producing DNA nanostructures. Here, we report a method for folding a custom template strand by binding individual staple sequences to multiple locations on the template. We built several nanostructures for well-controlled testing of various design rules, and demonstrate folding of a 6-kb template by as few as 10 unique strand sequences binding to 10 ± 2 locations on the template strand.
authors.affiliation: Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA.
authors.forename: Stefan
authors.initials: S
authors.lastname: Niekamp
authors.affiliation: Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA.
authors.forename: Katy
authors.initials: K
authors.lastname: Blumer
authors.affiliation: Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA.
authors.forename: Parsa M
authors.initials: PM
authors.lastname: Nafisi
authors.affiliation: Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA.
authors.forename: Kathy
authors.initials: K
authors.lastname: Tsui
authors.affiliation: Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA.
authors.forename: John
authors.initials: J
authors.lastname: Garbutt
authors.affiliation: Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA shawn.douglas@ucsf.edu.
authors.forename: Shawn M
authors.identifier: 0000-0001-5398-9041
authors.initials: SM
authors.lastname: Douglas
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D049329:Nanostructures; D036103:Nanotechnology; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry
chemical_list: D009841:Oligonucleotides; D004247:DNA