title: [STUDY OF COMPOSITION OF PLANT EXTRACTS, POSSESSING ANTIMICROBIAL EFFECT AGAINST VIBRIO CHOLERAE EL TOR, USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY].
abstract: OBJECTIVE
  Study the composition of plant extracts using high-performance liquid chromatography. (HPLC) and evaluation of their antimicrobial effect against Vibrio cholerae El Tor.
  
  
  METHODS
  Qualitative and quantitative composition of plant extracts was studied using HPLC. Determination of sensitivity of microorganisms to plant extracts was carried out by diffusion into agar method and serial dilutions method.
  
  
  RESULTS
  Antibacterial effect of water, water-alcohol and acetone extracts of roots of Limonium gmelinii L., Berberis vulgaris L. and Glycyrrhiza glabra L. was studied. The most effective methods of extraction of biologically active substances, possessing antimicrobial effect against various strains of V. cholerae El Tor, were determined.
  
  
  CONCLUSIONS
  The use of HPLC allowed to establish the presence of catechines, alkaloids protoberberines and glycyrrhizic acid in xtracts, possessing antimicrobial effect against V. cholera El Tor strains.
authors.forename: O V
authors.initials: OV
authors.lastname: Markina
authors.forename: E V
authors.initials: EV
authors.lastname: Maksimenko
authors.forename: N V
authors.initials: NV
authors.lastname: Markin
authors.forename: N A
authors.initials: NA
authors.lastname: Selyanskaya
authors.forename: A I
authors.initials: AI
authors.lastname: Shelokhovich
authors.forename: A B
authors.initials: AB
authors.lastname: Mazrukho
authors.forename: N I
authors.initials: NI
authors.lastname: Borisenko
mesh_terms: D000096:Acetone / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001600:Berberine Alkaloids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D028142:Berberis / Q000737:chemistry; D002396:Catechols / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000737:chemistry; D006035:Glycyrrhiza / Q000737:chemistry; D019695:Glycyrrhizic Acid / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry*; D029606:Plumbaginaceae / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D014734:Vibrio cholerae / Q000187:drug effects* / Q000254:growth & development; D014867:Water / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D001600:Berberine Alkaloids; D002396:Catechols; D010936:Plant Extracts; D012997:Solvents; D014867:Water; D000096:Acetone; D000431:Ethanol; D019695:Glycyrrhizic Acid