title: Plasma Peptidome as a Source of Biomarkers for Diagnosis of Cholangiocarcinoma.
abstract: Cholangiocarcinoma (CCA) is the bile duct cancer which constitutes one of the important public health problems in Thailand with high mortality rate, especially in the Opisthorchis viverrini (a parasite risk factor for CCA) endemic area of the northeastern region of the country. This study aimed to identify potential biomarkers from the plasma peptidome by CCA patients. Peptides were isolated using 10 kDa cut-off filter column and the flow-through was then used as a peptidome for LC-MS/MS analysis. A total of 209 peptides were obtained. Among these, 15 peptides were concerned with signaling pathways and 12 related to metabolic, regulatory, and biosynthesis of secondary metabolite pathways. Five exclusive peptides were identified as potential biomarkers, i.e. ETS domain-containing transcription factor ERF (P50548), KIAA0220 (Q92617), phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform isoform 1 (P42338), LP2209 (Q6XYC0), and casein kinase II subunit alpha (P19784). Three of these biomarkers are signaling related molecules. A combination of these biomarkers for CCA diagnosis is proposed.
authors.affiliation: Graduate Program in Bioclinical Sciences, Chulabhorn International College of Medicine, Thammasat University, Pathumthani, Thailand E-mail : kesaratmu@yahoo.com.
authors.forename: Kanawut
authors.initials: K
authors.lastname: Kotawong
authors.forename: Veerachai
authors.initials: V
authors.lastname: Thitapakorn
authors.forename: Sittiruk
authors.initials: S
authors.lastname: Roytrakul
authors.forename: Narumon
authors.initials: N
authors.lastname: Phaonakrop
authors.forename: Vithoon
authors.initials: V
authors.lastname: Viyanant
authors.forename: Kesara
authors.initials: K
authors.lastname: Na-Bangchang
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D001650:Bile Duct Neoplasms / Q000097:blood* / Q000469:parasitology / Q000473:pathology; D001653:Bile Ducts, Intrahepatic / Q000378:metabolism* / Q000469:parasitology / Q000473:pathology; D014408:Biomarkers, Tumor / Q000097:blood*; D016022:Case-Control Studies; D018281:Cholangiocarcinoma / Q000097:blood* / Q000469:parasitology / Q000473:pathology; D002853:Chromatography, Liquid; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D009889:Opisthorchiasis / Q000150:complications* / Q000453:epidemiology / Q000469:parasitology; D009891:Opisthorchis / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000097:blood*; D011379:Prognosis; D012307:Risk Factors; D053719:Tandem Mass Spectrometry; D013785:Thailand / Q000453:epidemiology
chemical_list: D014408:Biomarkers, Tumor; D010446:Peptide Fragments