title: Anti-human AFP variant monoclonal antibody in radioimmunodetection of primary hepatocellular carcinoma.
abstract: OBJECTIVE
  To investigate the affinity of AFP-R-LCA monoclonal antibody (AFP-R-LCA McAb) for AFP-positive primary hepatocellular carcinoma (HCC) cells.
  
  
  METHODS
  AFP-R-LCA McAb was labeled by (131)I. Eleven cases of HCC with AFP positivity, 6 with AFP negativity, and 4 with hepatitis B-related cirrhosis were investigated by radioimmunodetection.
  
  
  RESULTS
  The (131)I-AFP-R-LCA McAb immunoreacted with 9 of the HCC AFP-positive cases (9/11), but with none of the 6 AFP negative HCC cases or of the 4 cirrhosis patients. (131)I-AFP-R-LCA McAb at a small dose (7.4 × 10(7) Bq/300 μg) was associated with no side effects as determined by the liver function test, prothrombin time (Pt) test and thyroid gland function test (P > 0.05). Two cases of AFP-positive HCC were not imaged because of large tumor size (diameter > 10 cm) and higher AFP concentration in serum (20000 μg/L).
  
  
  CONCLUSIONS
  AFP-R-LCA McAb has a strong and special affinity to AFP-positive HCC cells and may be useful as a carrier for radioimmunodetection and radioimmunotherapy.
authors.affiliation: Yang Liu, Meng-Chao Wu, Han Chen, Bai-He Zhang, Guang-Xiang Qian, Wen-Zhou Pan, Mei-Yu Qiang, Eastern Hospital of Hepatobiliary Surgery, Second Military Medical University, Shanghai 200433, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Yang Liu, Meng-Chao Wu, Han Chen, Bai-He Zhang, Guang-Xiang Qian, Wen-Zhou Pan, Mei-Yu Qiang, Eastern Hospital of Hepatobiliary Surgery, Second Military Medical University, Shanghai 200433, China.
authors.forename: M C
authors.initials: MC
authors.lastname: Wu
authors.affiliation: Yang Liu, Meng-Chao Wu, Han Chen, Bai-He Zhang, Guang-Xiang Qian, Wen-Zhou Pan, Mei-Yu Qiang, Eastern Hospital of Hepatobiliary Surgery, Second Military Medical University, Shanghai 200433, China.
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.affiliation: Yang Liu, Meng-Chao Wu, Han Chen, Bai-He Zhang, Guang-Xiang Qian, Wen-Zhou Pan, Mei-Yu Qiang, Eastern Hospital of Hepatobiliary Surgery, Second Military Medical University, Shanghai 200433, China.
authors.forename: B H
authors.initials: BH
authors.lastname: Zhang
authors.affiliation: Yang Liu, Meng-Chao Wu, Han Chen, Bai-He Zhang, Guang-Xiang Qian, Wen-Zhou Pan, Mei-Yu Qiang, Eastern Hospital of Hepatobiliary Surgery, Second Military Medical University, Shanghai 200433, China.
authors.forename: G X
authors.initials: GX
authors.lastname: Qian
authors.affiliation: Yang Liu, Meng-Chao Wu, Han Chen, Bai-He Zhang, Guang-Xiang Qian, Wen-Zhou Pan, Mei-Yu Qiang, Eastern Hospital of Hepatobiliary Surgery, Second Military Medical University, Shanghai 200433, China.
authors.forename: W Z
authors.initials: WZ
authors.lastname: Pan
authors.affiliation: Yang Liu, Meng-Chao Wu, Han Chen, Bai-He Zhang, Guang-Xiang Qian, Wen-Zhou Pan, Mei-Yu Qiang, Eastern Hospital of Hepatobiliary Surgery, Second Military Medical University, Shanghai 200433, China.
authors.forename: M Y
authors.initials: MY
authors.lastname: Qiang
keywords: AFP; Liver neoplasms; McAb; Radioimmunodetection