title: HSP-1/2, a major horse seminal plasma protein, acts as a chaperone against oxidative stress.
abstract: The major protein of equine seminal plasma, HSP-1/2 exhibits chaperone-like activity and protects a variety of target proteins against thermal and chemical stress conditions. Here, we show that HSP-1/2 is able to protect enzymes such as alcohol dehydrogenase and glucose-6-phosphate dehydrogenase against H2O2 induced stress, clearly demonstrating that HSP-1/2 acts as a chaperone against oxidative stress. Further, the present studies show that HSP-1/2 also inhibits lipid (linoleic acid) peroxidation by hydroxyl radicals in vitro. These results are of great significance considering that so far limited or no antioxidative mechanism has been reported to be present in the mammalian spermatozoa that prevents lipid peroxidation which is detrimental to the motility and functioning of spermatozoa.
authors.affiliation: School of Chemistry, University of Hyderabad, Hyderabad 500046, India.
authors.forename: C Sudheer
authors.initials: CS
authors.lastname: Kumar
authors.affiliation: School of Chemistry, University of Hyderabad, Hyderabad 500046, India. Electronic address: mjswamy1@gmail.com.
authors.forename: Musti J
authors.initials: MJ
authors.lastname: Swamy
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D006736:Horses; D015227:Lipid Peroxidation / Q000502:physiology*; D018832:Molecular Chaperones; D018384:Oxidative Stress / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012661:Semen / Q000378:metabolism*; D029589:Seminal Plasma Proteins / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D006023:Glycoproteins; C095321:HSP-1 protein, Equus caballus; C106240:HSP-2 protein, Equus caballus; D018832:Molecular Chaperones; D017382:Reactive Oxygen Species; D029589:Seminal Plasma Proteins
keywords: Capacitation; Lipid peroxidation; Membranolytic activity; Molecular chaperone; Oxidative stress