title: Efficacy of Hyaluronic Acid in The Selection of Human Spermatozoa with Intact DNA by The Swim-up Method.
abstract: OBJECTIVE
  In 2014, enrolled 20 patients who applied to the Unit of Assisted Reproduction Techniques, Konya Necmettin Erbakan University. Based on the presence of hyaluronic acid (HA) in the oocyte-cumulus cell complex, sperm attached to HA in vivo were modeled in vitro. Available healthy sperm obtained in the swim-up procedure using HA were investigated.
  
  
  METHODS
  This observational cohort study, a routine analysis was conducted on the ejaculation samples obtained from 20 patients. We divided each sample into two groups and the swim-up method was applied. Human serum albumin (HSA, 0.5%) was added to samples from the first group. HA (10%) was added to samples from the second group. We determined the floating linear and non-linear sperm concentrations of both groups annexin V was used to determine the rate of apoptosis of these sperm.
  
  
  RESULTS
  Following swim-up, linear and non-linear sperm concentrations were higher in the group that contained HA compared to the group with HSA. However, there was a significantly higher apoptosis rate in the HSA group compared to the HA group.
  
  
  CONCLUSIONS
  The addition of HA to the medium in the swim-up procedure positively affected sperm parameters. Thus, healthier sperm cells were obtained without DNA damage and with high motility.
authors.affiliation: Necmettin Erbakan (NE) University, Meram Faculty of Medicine, Department of Histology and Embryology, Konya, Turkey.
authors.forename: Aslihan
authors.initials: A
authors.lastname: Saylan
authors.affiliation: Necmettin Erbakan (NE) University, Meram Faculty of Medicine, Department of Histology and Embryology, Konya, Turkey.
authors.forename: Selcuk
authors.initials: S
authors.lastname: Duman
keywords: Annexin V; Apoptosis; Human; Hyaluronic Acid