title: [Sunitinib inhibits the expressions of co-stimulatory molecule ligands on dendritic cells].
abstract: OBJECTIVE
  To investigate the effect of sunitinib on the expressions of co-stimulatory molecule ligands, programmed death ligand 1 (PD-L1), PD-L2, CD80, CD86, B7-H4 and herpes virus entry mediator (HVEM) on peripheral blood monocyte-derived dendritic cells (DCs) from patients with renal cell carcinoma (RCC).
  
  
  METHODS
  Monocyte-derived DCs from patients with RCC were cultured in vitro and randomly divided into three groups: sunitinib combined with lipopolysaccharide (LPS), LPS only and dimethyl sulfoxide (DMSO) treatment. Sunitinib plus LPS group was pretreated with 200 ng/mL sunitinib for 12 hours followed by stimulated with 1 μg/mL LPS for 24 hours; LPS group was pretreated with 1 μL/mL DMSO for 12 hours and then stimulated with 1 μg/mL LPS for 24 hours; DMSO group was treated with 1 μL/mL DMSO for 36 hours. Morphological changes were observed by an inverted microscope. Flow cytometry was used to detect the expressions of PD-L1, PD-L2, CD80, CD86, B7-H4 and HVEM.
  
  
  RESULTS
  Sunitinib-LPS co-treated and LPS-treated cells had typical dendrites, while DMSO-treated cells had no obvious dendrites. Compared with the LPS-treated group, the expressions of CD80, PD-L1 and B7-H4 on DCs significantly decreased in the sunitinib-LPS group; the expressions of PD-L1, PD-L2, CD80, CD86, B7-H4 and HVEM were lower in the DMSO group.
  
  
  CONCLUSIONS
  Sunitinib inhibits the expressions of CD80, PD-L1 and B7-H4 on DCs induced by LPS.
authors.affiliation: Department of Urology, Clinical College, No. 307 Hospital of PLA, Anhui Medical University, Beijing 100071, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Ding
authors.affiliation: Department of Urology, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Haixing
authors.initials: H
authors.lastname: Mai
authors.affiliation: Department of Urology, Clinical College, No. 307 Hospital of PLA, Anhui Medical University, Department of Urology, Academy of Military Medical Sciences, Beijing 100071, China. *Corresponding authors, E-mail: chenlj829@163.com.
authors.forename: Lijun
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Hematopoietic Stem Cell Transplantation, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China. *Corresponding authors, E-mail: zb307ctc@163.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
mesh_terms: D018122:B7-1 Antigen / Q000235:genetics / Q000276:immunology; D051940:B7-2 Antigen / Q000235:genetics / Q000276:immunology; D060890:B7-H1 Antigen / Q000235:genetics / Q000276:immunology; D002292:Carcinoma, Renal Cell / Q000188:drug therapy / Q000235:genetics* / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology; D015536:Down-Regulation / Q000187:drug effects; D005260:Female; D006801:Humans; D007211:Indoles / Q000009:adverse effects / Q000494:pharmacology*; D008024:Ligands; D008297:Male; D009000:Monocytes / Q000187:drug effects / Q000276:immunology; D061027:Programmed Cell Death 1 Ligand 2 Protein / Q000235:genetics / Q000276:immunology; D011758:Pyrroles / Q000009:adverse effects / Q000494:pharmacology*; D000077210:Sunitinib
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; D060890:B7-H1 Antigen; C423236:CD274 protein, human; D007211:Indoles; D008024:Ligands; D061027:Programmed Cell Death 1 Ligand 2 Protein; D011758:Pyrroles; D000077210:Sunitinib