title: Comparative study of different alcoholate pretreatments for enhanced enzymatic hydrolysis of sugarcane bagasse.
abstract: Pretreatment of sugarcane bagasse (SCB) with alcoholates, sodium methoxide (CH3ONa), potassium methoxide (CH3OK) and sodium ethoxide (C2H5ONa), was investigated. Analyses of lignocellulose composition and enzymatic saccharification indicated that C2H5ONa showed the highest enzymatic efficiency of 102.1%. The response surface optimization of C2H5ONa pretreatment showed that under optimal conditions (4% of C2H5ONa, 121°C, 1h), 65.4% of lignin was removed and the enzymatic efficiency reached 105.2%. Hydrolysis of SCB with cellulases and xylanase at a ratio of 4:1 showed the strongest synergism with reducing sugar production of 21g/L and conversion rates of cellulose and xylan reaching 110.4% and 94.5%, respectively. These results indicated that C2H5ONa is a promising alkali to pretreat SCB and the synergism between cellulases and xylanase has a significant effect on enzymatic saccharification of the pretreated SCB.
authors.affiliation: Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
authors.forename: Qiuli
authors.initials: Q
authors.lastname: Yan
authors.affiliation: Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Fu
authors.affiliation: Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Lv
authors.affiliation: Research and Development Department, Guangdong Qizhi Biotechnology Co., Ltd., Guangzhou, PR China.
authors.forename: Chunjiang
authors.initials: C
authors.lastname: Xiong
authors.affiliation: Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China. Electronic address: jianghai.lin@jnu.edu.cn.
authors.forename: Jianghai
authors.initials: J
authors.lastname: Lin
authors.affiliation: Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China. Electronic address: zhliu@jnu.edu.cn.
authors.forename: Zehuan
authors.initials: Z
authors.lastname: Liu
mesh_terms: D002241:Carbohydrates / Q000032:analysis; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D000431:Ethanol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006868:Hydrolysis; D000432:Methanol / Q000494:pharmacology*; D031786:Saccharum / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
chemical_list: D002241:Carbohydrates; C098088:sodium ethoxide; D000431:Ethanol; D002482:Cellulose; C027433:bagasse; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases; D000432:Methanol
keywords: Alcoholate pretreatment; Enzymatic hydrolysis; Response surface methodology; Sugarcane bagasse; Synergism