title: Effects of dietary addition of cellulase and a Saccharomyces cerevisiae fermentation product on nutrient digestibility, rumen fermentation and enteric methane emissions in growing goats.
abstract: This study was designed to assess the effectiveness of dietary cellulase (243 U/g, derived from Neocallimastix patriciarum) and a Saccharomyces cerevisiae fermentation product (yeast product) on ruminal fermentation characteristics, enteric methane (CH4) emissions and methanogenic community in growing goats. The experiment was conducted in a 5 × 5 Latin square design using five Xiangdong black wether goats. The treatments included a Control and two levels of cellulase (0.8 g and 1.6 g/kg dry matter intake (DMI), i.e. 194 U/kg and 389 U/kg DMI, respectively) crossed over with two levels (6 g or 12 g/kg DMI) of the yeast product. There were no significant differences regarding feed intake, apparent digestibility of organic matter, neutral detergent fibre and acid detergent fibre among all the treatments. In comparison with the Control, the ruminal ammonia N concentration was decreased (p = 0.001) by cellulase and yeast product addition. The activities of carboxymethylcellulase and xylanase were decreased after cellulase addition. Moreover, dietary cellulase and yeast product addition led to a significant reduction (p < 0.05) of enteric CH4 emissions although the diversity and copy numbers of methanogens among treatments were not dissimilar. The present results indicate that the combination of cellulase and yeast fermentation product can reduce the production of CH4 energy and mitigate the enteric CH4 emissions to a certain degree.
authors.affiliation: a Key Laboratory for Agro-Ecological Processes in Subtropical Region, and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture , Institute of Subtropical Agriculture, The Chinese Academy of Sciences , Changsha , Hunan , P.R. China.|b College of Resources and Environment , University of Chinese Academy of Sciences , Beijing , P. R. China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Lu
authors.affiliation: a Key Laboratory for Agro-Ecological Processes in Subtropical Region, and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture , Institute of Subtropical Agriculture, The Chinese Academy of Sciences , Changsha , Hunan , P.R. China.|b College of Resources and Environment , University of Chinese Academy of Sciences , Beijing , P. R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.affiliation: a Key Laboratory for Agro-Ecological Processes in Subtropical Region, and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture , Institute of Subtropical Agriculture, The Chinese Academy of Sciences , Changsha , Hunan , P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: a Key Laboratory for Agro-Ecological Processes in Subtropical Region, and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture , Institute of Subtropical Agriculture, The Chinese Academy of Sciences , Changsha , Hunan , P.R. China.
authors.forename: Chuanshe
authors.initials: C
authors.lastname: Zhou
authors.affiliation: a Key Laboratory for Agro-Ecological Processes in Subtropical Region, and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture , Institute of Subtropical Agriculture, The Chinese Academy of Sciences , Changsha , Hunan , P.R. China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Han
authors.affiliation: c Animal Production and Health Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture , International Atomic Energy Agency , Vienna , Austria.
authors.forename: Edwin Nicholas
authors.initials: EN
authors.lastname: Odongo
authors.affiliation: a Key Laboratory for Agro-Ecological Processes in Subtropical Region, and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture , Institute of Subtropical Agriculture, The Chinese Academy of Sciences , Changsha , Hunan , P.R. China.
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Tan
authors.affiliation: a Key Laboratory for Agro-Ecological Processes in Subtropical Region, and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture , Institute of Subtropical Agriculture, The Chinese Academy of Sciences , Changsha , Hunan , P.R. China.
authors.forename: Shaoxun
authors.initials: S
authors.lastname: Tang
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002480:Cellulase / Q000737:chemistry / Q000378:metabolism*; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements / Q000032:analysis; D005285:Fermentation; D005656:Fungal Proteins / Q000008:administration & dosage / Q000737:chemistry; D000069196:Gastrointestinal Microbiome / Q000502:physiology; D006041:Goats / Q000382:microbiology / Q000502:physiology*; D008297:Male; D008697:Methane / Q000378:metabolism*; D020088:Neocallimastix / Q000737:chemistry*; D012417:Rumen / Q000382:microbiology / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000737:chemistry*
chemical_list: D005656:Fungal Proteins; D002480:Cellulase; D008697:Methane
keywords: cellulase; digestibility; feed supplements; methane production; ruminants; yeast