title: [Knockdown of CART1 induces S phase arrest and inhibits invasion and migration of MDA-MB-231 breast cancer cells].
abstract: OBJECTIVE
  To investigate the effect of exogenous small interference RNA(siRNA)-mediated silencing of the cartilage homeoprotein 1 (CART1) gene on cell biological behavior in MDA-MB-231 human breast cancer cells.
  
  
  METHODS
  We designed and chemically synthesized siRNA targeting CART1 or control, and then siRNA was transfected into MDA-MB-231 cells using RNAiMAX. The suppression effect of siRNA on CART1 expression was determined by real-time quantitative PCR and Western blotting. The effects of CART1 knockdown on MDA-MB-231 cell invasion, proliferation and cell cycle were detected by TranswellTM invasion assay, CCK-8 assay and flow cytometry, respectively.
  
  
  RESULTS
  CART1-siRNA could significantly suppress the expression of CART1 mRNA and protein as compared with the control group. MDA-MB-231 cells transfected with CART1 siRNA had lower capacity of invasion and proliferation compared with the control group. What's more, the cell ratio increased in S phase and decreased in G2/M in the CART1 siRNA-transfected cells.
  
  
  CONCLUSIONS
  Knockdown of the CART1 gene could significantly inhibit cell invasion and proliferation and induce cell cycle arrest in S phase in MDA-MB-231 human breast cancer cells.
authors.affiliation: Department of Pathology, Centre for Molecular Medicine and Cancer Research, College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Tinghong
authors.initials: T
authors.lastname: Guan
authors.affiliation: Department of Pathology, Centre for Molecular Medicine and Cancer Research, College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Linxi
authors.initials: L
authors.lastname: Su
authors.affiliation: Department of Pathology, Centre for Molecular Medicine and Cancer Research, College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China. *Corresponding author, E-mail: yiyongfen1953@aliyun.com.
authors.forename: Yongfen
authors.initials: Y
authors.lastname: Yi
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D009361:Neoplasm Invasiveness; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D059807:S Phase Cell Cycle Checkpoints; D014162:Transfection
chemical_list: C489985:ALX1 protein, human; D018398:Homeodomain Proteins; D034741:RNA, Small Interfering