title: Effect of an Activated Platelet Concentrate on Differentiated Cells Involved in Tissue Healing.
abstract: Tissue healing is a complex process involving several players such as cells and growth factors released from platelets upon activation. Today, platelet concentrates (PCs) are used in many different medical fields including oral, orthopaedic, and reconstructive surgery since they allow growth factors delivery to the injured site, aiming at enhancing tissue regeneration. The purpose of this in vitro study was to evaluate the effect of the acellular plasma of an activated platelet concentrate obtained using a manual protocol, on the proliferation, and biological activity of differentiated cells involved in tissue healing. Human osteoblasts and dermal fibroblasts were grown in serum-free medium supplemented with PC derived from several donors. Human osteoblast and human dermal fibroblast proliferation was assessed by MTT test after 7 days and cells were count up to 12-day incubation. Human osteoblast osteo-differentiation was tested after 7 and 14-day incubation by alkaline phosphatase assay. The addition of PC to the culture medium caused an increased proliferation with respect to cells grown in standard condition. The results of the present study suggest that PC supports the proliferation of terminally differentiated cells involved in wound healing and tissue regeneration, confirming its beneficial clinical application in regenerative therapies.
authors.affiliation: *Department of Biomedical, Surgical and Dental Sciences, University of Milan†IRCCS Galeazzi Orthopedic Institute, Milan‡Department of Health Sciences "A. Avogadro," University of Eastern Piedmont, Novara, Italy.
authors.forename: Anna T
authors.initials: AT
authors.lastname: Brini
authors.forename: Caterina
authors.initials: C
authors.lastname: Ceci
authors.forename: Silvio
authors.initials: S
authors.lastname: Taschieri
authors.forename: Stefania
authors.initials: S
authors.lastname: Niada
authors.forename: Alessandra
authors.initials: A
authors.lastname: Lolato
authors.forename: Chiara
authors.initials: C
authors.lastname: Giannasi
authors.forename: Carmen
authors.initials: C
authors.lastname: Mortellaro
authors.forename: Massimo
authors.initials: M
authors.lastname: Del Fabbro
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001792:Blood Platelets / Q000502:physiology*; D002454:Cell Differentiation; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D010006:Osteoblasts / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology; D015539:Platelet Activation / Q000502:physiology*; D053657:Platelet-Rich Plasma / Q000502:physiology*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D000469:Alkaline Phosphatase