title: A Novel Method for the Separation of Overlapping Pollen Species for Automated Detection and Classification.
abstract: The identification of pollen in an automated way will accelerate different tasks and applications of palynology to aid in, among others, climate change studies, medical allergies calendar, and forensic science. The aim of this paper is to develop a system that automatically captures a hundred microscopic images of pollen and classifies them into the 12 different species from Lagunera Region, Mexico. Many times, the pollen is overlapping on the microscopic images, which increases the difficulty for its automated identification and classification. This paper focuses on a method to segment the overlapping pollen. First, the proposed method segments the overlapping pollen. Second, the method separates the pollen based on the mean shift process (100% segmentation) and erosion by H-minima based on the Fibonacci series. Thus, pollen is characterized by its shape, color, and texture for training and evaluating the performance of three classification techniques: random tree forest, multilayer perceptron, and Bayes net. Using the newly developed system, we obtained segmentation results of 100% and classification on top of 96.2% and 96.1% in recall and precision using multilayer perceptron in twofold cross validation.
authors.affiliation: Departamento de Posgrado, Instituto Tecnológico Superior de Lerdo, Tecnológico 1555, Placido Domingo, 35150 Lerdo, DG, Mexico; Departamento de Posgrado, Instituto Tecnológico de la Laguna, Boulevard Revolución, Centro, 27000 Torreón, CO, Mexico.
authors.forename: Santiago
authors.identifier: 0000-0002-2575-8251
authors.initials: S
authors.lastname: Tello-Mijares
authors.affiliation: Departamento de Posgrado, Instituto Tecnológico de la Laguna, Boulevard Revolución, Centro, 27000 Torreón, CO, Mexico.
authors.forename: Francisco
authors.initials: F
authors.lastname: Flores
mesh_terms: D000465:Algorithms; D001499:Bayes Theorem; D001901:Botany / Q000379:methods*; D057231:Climate Change; D003116:Color; D019295:Computational Biology / Q000379:methods; D005189:False Positive Reactions; D006801:Humans; D007091:Image Processing, Computer-Assisted; D000069550:Machine Learning; D008800:Mexico; D010363:Pattern Recognition, Automated; D011058:Pollen / Q000145:classification*; D011336:Probability; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013045:Species Specificity