title: New test for endothelin receptor type B (EDNRB) mutation genotyping in horses.
abstract: Lethal white foal syndrome (LWFS) is an autosomal recessive disease of neonatal foals characterized by a white hair coat and a functional intestinal obstruction. Traditional techniques for identifying the dinucleotide mutation (TC→AG) of the endothelin receptor B gene (EDNRB) associated with LWFS are time-consuming. We developed a new technique based on mutagenically separated polymerase chain reaction (MS-PCR) for simple detection of the EDNRB genotype in horses.
authors.affiliation: Departamento de Producción Animal, División de Ciencias Veterinarias, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, A.P. 218 Zapopan 1, C.P. 45101, Zapopan, Jalisco, Mexico. Electronic address: manayala@cucba.udg.mx.
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Ayala-Valdovinos
authors.affiliation: Departamento de Producción Animal, División de Ciencias Veterinarias, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, A.P. 218 Zapopan 1, C.P. 45101, Zapopan, Jalisco, Mexico.
authors.forename: Jorge
authors.initials: J
authors.lastname: Galindo-García
authors.affiliation: Departamento de Producción Animal, División de Ciencias Veterinarias, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, A.P. 218 Zapopan 1, C.P. 45101, Zapopan, Jalisco, Mexico.
authors.forename: David
authors.initials: D
authors.lastname: Sánchez-Chiprés
authors.affiliation: Departamento de Producción Animal, División de Ciencias Veterinarias, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, A.P. 218 Zapopan 1, C.P. 45101, Zapopan, Jalisco, Mexico.
authors.forename: Theodor
authors.initials: T
authors.lastname: Duifhuis-Rivera
mesh_terms: D000818:Animals; D004587:Electrophoresis, Agar Gel; D060005:Genotyping Techniques / Q000379:methods*; D006734:Horse Diseases / Q000235:genetics; D006736:Horses / Q000235:genetics*; D016296:Mutagenesis / Q000235:genetics; D009154:Mutation / Q000235:genetics*; D044023:Receptor, Endothelin B / Q000235:genetics*
chemical_list: D044023:Receptor, Endothelin B
keywords: EDNRB gene; Horse; LWFS; Mutagenically separated polymerase chain reaction