title: [Evaluation of Salt Tolerance of Transgenic Tobacco Plants Bearing with P5CS1 Gene of Arabidopsis thaliana].
abstract: Arabidopsis thaliana delta1-pyrroline-5-carhoxylate synthase 1 gene (P5CS1) cDNA was cloned under the control of the potent constitutive 35S RNA promoter of the cauliflower mosaic virus and transferred into genome of tobacco cv. Petit Havana SR-1 (Nicotiana tabacum L.) plants. It is shown that the constitutive level of proline in the transgenic plants T0 exceeds that of the SR1 reference line by 1.5 to 4 times. Under conditions of salt stress (200, 300 mM NaCl) T1-generation transgenic plants in early stages of development formed a large biomass, developed more quickly, and had a higher rate of root growth compared to the control, which confirms the involvement of the P5CS1 gene in molecular mechanisms of stress resistance in plants.
authors.forename: S M
authors.initials: SM
authors.lastname: Ibragimova
authors.forename: E A
authors.initials: EA
authors.lastname: Trifonova
authors.forename: E A
authors.initials: EA
authors.lastname: Filipenko
authors.forename: V K
authors.initials: VK
authors.lastname: Shymny
mesh_terms: D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000096:biosynthesis* / Q000235:genetics; D050698:Glutamate-5-Semialdehyde Dehydrogenase / Q000096:biosynthesis* / Q000235:genetics; D009097:Multienzyme Complexes / Q000096:biosynthesis* / Q000235:genetics; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000096:biosynthesis* / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development*; D055049:Salt Tolerance / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics / Q000254:growth & development*
chemical_list: D029681:Arabidopsis Proteins; D009097:Multienzyme Complexes; C583835:delta(1)-pyrroline-5-carboxylate synthetase, Arabidopsis; D050698:Glutamate-5-Semialdehyde Dehydrogenase; D017853:Phosphotransferases (Alcohol Group Acceptor)