title: Piperlongumine inhibits gastric cancer cells via suppression of the JAK1,2/STAT3 signaling pathway.
abstract: Piperlongumine (PL), a major active component of long peppers, has been reported to possess anti‑cancer properties; however, its effect on gastric cancer (GC) has remained to be demonstrated. The present study assessed the effects of PL on the MKN45 and AGS GC cell lines and explored the underlying mechanisms. An MTT assay revealed that PL suppressed the proliferation of GC cells, while flow cytometric analysis showed that PL inhibited cell cycle progression. Furthermore, Transwell assays revealed the inhibitory effects of PL on the invasion and migration of GC cells. In addition, PL reduced the phosphorylation of Janus kinase (JAK)1, JAK2 and signal transducer and activator of transcription (STAT)3 in a concentration‑dependent manner, as indicated by western blot analysis, and decreased the expression of STAT3‑dependent tumor‑associated genes in GC cells, as revealed by PCR analysis. In conclusion, the present study was the first, to the best of our knowledge, to reveal the efficacy of PL against GC. The consumption of long peppers is therefore recommended for the prevention and treatment of GC, and PL may be a promising candidate drug for treating GC.
authors.affiliation: Department of General Surgery, Tianjin Hospital, Tianjin 300211, P.R. China.
authors.forename: Baoji
authors.initials: B
authors.lastname: Song
authors.affiliation: Department of Gastric Cancer, Tianjin Cancer Hospital, Key Laboratory of Cancer Prevention and Treatment of Tianjin, Tianjin Medical University, Tianjin 300060, P.R. China.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Zhan
authors.affiliation: Department of General Surgery, Tianjin Hospital, Tianjin 300211, P.R. China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Bian
authors.affiliation: Department of General Surgery, Tianjin Hospital, Tianjin 300211, P.R. China.
authors.forename: Junping
authors.initials: J
authors.lastname: Gu
mesh_terms: D045744:Cell Line, Tumor; D004148:Dioxolanes / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D053613:Janus Kinase 1 / Q000235:genetics / Q000378:metabolism*; D053614:Janus Kinase 2 / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects / Q000235:genetics; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects* / Q000235:genetics; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D010881:Piperidones
chemical_list: D004148:Dioxolanes; D053613:Janus Kinase 1; D053614:Janus Kinase 2; D009363:Neoplasm Proteins; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; C507904:JAK1 protein, human; C507924:JAK2 protein, human; C498077:piperlongumine; D010881:Piperidones