title: Silence of long noncoding RNA UCA1 inhibits malignant proliferation and chemotherapy resistance to adriamycin in gastric cancer.
abstract: Urothelial carcinoma associated 1 (UCA1) gene is a long noncoding RNA (lncRNA), which shows expression and function abnormality in some kinds of malignant cancers. This study aimed to investigate its clinical significance and to examine its regulative effects of malignant proliferation and chemosensitivity to doxorubicin in gastric cancer cells.
  
  
  
  The expression of UCA1 was detected by quantitative real-time PCR. Statistical analysis was used to determine the relationship between UCA1 expression and clinical features and the prognostic value of UCA1 for disease-free survival. MTT assay was used to detect cell proliferation ability and chemosensitivity. Dual-color flow cytometric method was used to detect cell apoptosis. The expression of cleaved PARP and Bcl-2 protein was examined by Western blot.
  
  
  
  UCA1 was highly expressed in gastric cancer tissues and cells, and its high expression level had a positive correlation with some malignant pathological characteristics of gastric cancer, such as higher grade and poor differentiation. Moreover, UCA1 was an independent prognostic biomarker of disease-free survival for gastric cancer patients. Silence of UCA1 could significantly inhibit the cell proliferation of gastric cancer BGC-823 and SGC7901 cells. The chemotherapy resistance to adriamycin of SGC7901/ADR cells was depressed by UCA1 silence, and IC50 for adriamycin presented a conspicuous depression. UCA1 silence advanced apoptosis induced by adriamycin in SGC7901/ADR cells, up-regulated cleaved PARP protein expression and depressed the expression of anti-apoptosis protein Bcl-2. These results demonstrated that chemotherapy resistance changes induced by UCA1 silence might be mediated by the cell apoptosis pathway.
  
  
  
  In summary, lncRNA UCA1 was an independent prognostic biomarker of disease-free survival in gastric cancer patients and acted as an oncogene to regulate the malignant proliferation and resistance to adriamycin in gastric cancer cells. UCA1 might provide a new potential therapeutic target and stratagem for gastric cancer.
authors.affiliation: Department of Surgical Oncology, Affiliated First Hospital, China Medical University, Shenyang, 110004, China.|Department of Neurobiology, College of Basic Medicine, China Medical University, Shenyang, 110122, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Shang
authors.affiliation: Department of Central Laboratory, School of Stomatology, China Medical University, Shenyang, 110007, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Surgical Oncology, Affiliated First Hospital, China Medical University, Shenyang, 110004, China.
authors.forename: Jiaxing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Surgical Oncology, Affiliated First Hospital, China Medical University, Shenyang, 110004, China. huangbaojun2014@163.com.|, No. 155 Nanjing North Street, Heping District, Shenyang, 110001, Liaoning, China. huangbaojun2014@163.com.
authors.forename: Baojun
authors.initials: B
authors.lastname: Huang
mesh_terms: D000328:Adult; D000368:Aged; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects* / Q000235:genetics; D018572:Disease-Free Survival; D015536:Down-Regulation; D004317:Doxorubicin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm / Q000187:drug effects / Q000235:genetics; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005260:Female; D020868:Gene Silencing; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D062085:RNA, Long Noncoding / Q000235:genetics*; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D015854:Up-Regulation
chemical_list: D000903:Antibiotics, Antineoplastic; D014408:Biomarkers, Tumor; D062085:RNA, Long Noncoding; C515159:UCA1 RNA, human; D004317:Doxorubicin
keywords: Adriamycin; Chemotherapy; Gastric cancer; Long noncoding RNA; UCA1