title: Characterization of dengue virus 2 growth in megakaryocyte-erythrocyte progenitor cells.
abstract: Megakaryocyte-erythrocyte progenitor (MEP) cells are potential in vivo targets of dengue virus (DENV); the virus has been found associated with megakaryocytes ex vivo and platelets during DENV-induced thrombocytopenia. We report here that DENV serotype 2 (DENV2) propagates well in human nondifferentiated MEP cell lines (Meg01 and K562). In comparison to virus propagated in Vero cells, viruses from MEP cell lines had similar structure and buoyant density. However, differences in MEP-DENV2 stability and composition were suggested by distinct protein patterns in western blot analysis. Also, antibody neutralization of envelope domain I/II on MEP-DENV2 was reduced relative to that on Vero-DENV2. Infectious DENV2 was produced at comparable kinetics and magnitude in MEP and Vero cells. However, fewer virion structures appeared in electron micrographs of MEP cells. We propose that DENV2 infects and produces virus efficiently in megakaryocytes and that megakaryocyte impairment might contribute to dengue disease pathogenesis.
authors.affiliation: Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University, Atlanta, GA, USA.
authors.forename: Kristina B
authors.initials: KB
authors.lastname: Clark
authors.affiliation: Center for AIDS Research, Department of Pediatrics, Emory University School of Medicine and Veterans Affairs Medical Center, Atlanta, GA, USA.
authors.forename: Hui-Mien
authors.initials: HM
authors.lastname: Hsiao
authors.affiliation: Center for AIDS Research, Department of Pediatrics, Emory University School of Medicine and Veterans Affairs Medical Center, Atlanta, GA, USA.
authors.forename: Leda
authors.initials: L
authors.lastname: Bassit
authors.affiliation: Departments of Pediatrics, Pathology, Microbiology, and Immunology, Vanderbilt University, Nashville, TN, USA.
authors.forename: James E
authors.initials: JE
authors.lastname: Crowe
authors.affiliation: Center for AIDS Research, Department of Pediatrics, Emory University School of Medicine and Veterans Affairs Medical Center, Atlanta, GA, USA.
authors.forename: Raymond F
authors.initials: RF
authors.lastname: Schinazi
authors.affiliation: Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Guey Chuen
authors.initials: GC
authors.lastname: Perng
authors.affiliation: Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University, Atlanta, GA, USA; New Iberia Research Center, University of Louisiana at Lafayette, New Iberia, LA, USA.
authors.forename: Francois
authors.initials: F
authors.lastname: Villinger
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D002460:Cell Line; D002522:Chlorocebus aethiops; D003715:Dengue / Q000821:virology; D003716:Dengue Virus / Q000254:growth & development* / Q000276:immunology / Q000648:ultrastructure; D006801:Humans; D055015:Megakaryocyte-Erythroid Progenitor Cells / Q000821:virology*; D051379:Mice; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000276:immunology; D014779:Virus Replication
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000956:Antigens, Viral; D014759:Viral Envelope Proteins
keywords: Dengue virus; Electron microscopy; Erythrocyte; K562; MEP; Meg01; Megakaryocyte; Progenitor