title: Large Particle Sorting to Isolate Live Parasitic Nematode Eggs.
abstract: Traditional jet-in-air cell sorters have been designed and optimized to isolate small particles such as mammalian lymphocytes with an average diameter of 10 μm. We discuss the practical considerations of setting up a conventional jet-in-air cell sorter, using a 200-μm nozzle, to isolate the large parasitic nematode eggs of Nippostrongylus brasiliensis, with a maximum size of 60 μm. The eggs were separated based on light scattering properties, no fluorescent dye or molecule was required.
authors.affiliation: Malaghan Institute of Medical Research, Wellington, New Zealand.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Schmidt
authors.affiliation: Malaghan Institute of Medical Research, Wellington, New Zealand.
authors.forename: Tiffany
authors.initials: T
authors.lastname: Bouchery
authors.affiliation: Malaghan Institute of Medical Research, Wellington, New Zealand.
authors.forename: Graham
authors.initials: G
authors.lastname: Le Gros
authors.affiliation: Malaghan Institute of Medical Research, Wellington, New Zealand.
authors.forename: Kylie M
authors.initials: KM
authors.lastname: Price
mesh_terms: D000818:Animals; D005243:Feces / Q000469:parasitology; D005434:Flow Cytometry / Q000379:methods*; D051379:Mice; D009559:Nippostrongylus / Q000166:cytology*; D010063:Ovum / Q000166:cytology*; D010271:Parasites / Q000166:cytology; D011786:Quality Control; D051381:Rats; D013242:Sterilization
keywords: cell sorting; egg; large particle; live parasite; nematode