title: Dietary flaxseed oil and fish oil ameliorates renal oxidative stress, protein glycation, and inflammation in streptozotocin-nicotinamide-induced diabetic rats.
abstract: Protective and prophylactic effects of omega-3 fatty acids on oxidative stress and inflammation are well known. We assessed beneficial effects of flaxseed oil and fish oil on streptozotocin (65 mg/kg; i.p.)-nicotinamide (110 mg/kg; i.p.) induced diabetic rats by studying renal expression of antioxidant and inflammatory genes. Diabetic rats given 10 % flaxseed oil or 10 % fish oil diet for 35 days showed significant decrease in renal lipid peroxidation. Flaxseed oil diet resulted in up-regulation of renal superoxide dismutase-1 (SOD-1) (activity and expression) and glutathione peroxidase-1 (GPx-1) expression. Furthermore, both diets up-regulated catalase (CAT) (activity and expression) and down-regulated heme oxygenase-1 (HO-1) expression. Both diets were able to limit the renal advanced glycation end products (AGEs) formation and reduced receptor of AGE (RAGE) protein expression significantly. Expressions of interleukin-6 (IL-6) and NF-κB p65 subunit were down-regulated significantly by flaxseed oil or fish oil diet. The histological tubular injuries were also lowered by both diets. These results suggest that dietary ω-3 fatty acids may slow the progression of diabetic nephropathy (DN) associated with oxidative stress, glycation, and inflammation in the kidney.
authors.affiliation: Department of Pharmaceutical Biotechnology, Bharati Vidyapeeth Deemed University, Poona College of Pharmacy, Erandwane, Pune, Maharashtra, 411038, India.
authors.forename: Nivedita M
authors.initials: NM
authors.lastname: Jangale
authors.affiliation: Interactive Research School for Health Affairs, Bharati Vidyapeeth Deemed University, Pune, Maharashtra, 411043, India.
authors.forename: Prasad P
authors.initials: PP
authors.lastname: Devarshi
authors.affiliation: Proteomics Facility, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, Maharashtra, 411008, India.
authors.forename: Sneha B
authors.initials: SB
authors.lastname: Bansode
authors.affiliation: Proteomics Facility, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, Maharashtra, 411008, India.
authors.forename: Mahesh J
authors.initials: MJ
authors.lastname: Kulkarni
authors.affiliation: Department of Pharmaceutical Biotechnology, Bharati Vidyapeeth Deemed University, Poona College of Pharmacy, Erandwane, Pune, Maharashtra, 411038, India. aharsulkar@yahoo.com.
authors.forename: Abhay M
authors.initials: AM
authors.lastname: Harsulkar
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000178:diet therapy* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D004042:Dietary Fats, Unsaturated / Q000627:therapeutic use*; D015525:Fatty Acids, Omega-3 / Q000627:therapeutic use; D005395:Fish Oils / Q000627:therapeutic use*; D005786:Gene Expression Regulation; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors*; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D007668:Kidney / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008043:Linseed Oil / Q000627:therapeutic use*; D015227:Lipid Peroxidation; D008297:Male; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D009536:Niacinamide; D018384:Oxidative Stress; D011897:Random Allocation; D017208:Rats, Wistar; D000067759:Receptor for Advanced Glycation End Products / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D013311:Streptozocin; D000093242:Interleukin-6 Inhibitors
chemical_list: D015415:Biomarkers; D004042:Dietary Fats, Unsaturated; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D017127:Glycation End Products, Advanced; D015850:Interleukin-6; D008043:Linseed Oil; D016328:NF-kappa B; D009536:Niacinamide; D000067759:Receptor for Advanced Glycation End Products; D013311:Streptozocin; D000093242:Interleukin-6 Inhibitors
keywords: Diabetes; Fish oil; Flaxseed oil; Glycation; Kidney; Streptozotocin