title: [THE DNA DIAGNOSTIC OF PSEUDOMONAS INFECTION].
abstract: The modern laboratory diagnostic of pseudomonas infection, including bacteriologic analysis with subsequent identication of agent, is time-consuming and it is not every time effective because of modifiability of characteristics of the mentioned agent. The article presents the laboratory mode of identification of Pseudomonas aeruginosa based on electrophoresis analysis of DNA isolatedfrom wound samples or microbial mass grew on solid nutritional medium. The wound sample or pure growth is placed into physiological solution and is warmed under 990C. This action results in DNA releasing into supernatant which sample is underwent to standard horizontal electrophoresis in agarose gel with ethidium bromide. The presence on electrophoregrams of three bands of DNA fluorescent in ultraviolet with size of fragments about 10,000, 6000-8000a and less than 750 pairs of nucleotides testifies the presence of P. aeruginosa in the analyzed biomaterial. The given mode permits to identify both chromogenic and pigment-free strains of P. aeruginosa and to differentiate them of other representatives of non-fermentative gram-negative bacteria and most significant genera and species of family of Enterobacteriaceae. The identification of P. aeruginosa in wound sample using the proposed mode implementing without its bacteriological analysis, can be used as technique of express diagnostic of pseudomonas infection.
authors.forename: N F
authors.initials: NF
authors.lastname: Maltseva
authors.forename: O N
authors.initials: ON
authors.lastname: Vorobyeva
authors.forename: A D
authors.initials: AD
authors.lastname: Tarasko
authors.forename: E A
authors.initials: EA
authors.lastname: Pirogov
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D004755:Enterobacteriaceae / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D004756:Enterobacteriaceae Infections / Q000175:diagnosis* / Q000382:microbiology; D004996:Ethidium; D006358:Hot Temperature; D006801:Humans; D011552:Pseudomonas Infections / Q000175:diagnosis* / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D012965:Sodium Chloride / Q000737:chemistry; D014946:Wound Infection / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D012965:Sodium Chloride; D004996:Ethidium