title: Pivotal role of microRNA-9 in osteosarcoma tumorigenesis and tumor progression.
abstract: MicroRNA-9 (miR-9) has a well-established role in various tumors; the clinical significance and potential mechanism of miR-9 in human osteosarcoma (OS) has not been elucidated. The aim of this study was to investigate the mechanism and role of miR-9 expression in osteosarcoma cells. miR-9 expression in the OS cell line MG-63 and OS tissues was compared to that in a human osteoblastic cell line (hFOB 1.19) and adjacent normal tissues, respectively, by reverse transcriptase-polymerase chain reaction. miR-9 expression was downregulated by introducing small interfering RNA against miR-9 (si-miR-9) into the cells, and the proliferative, migratory, and invasive capacities of si-miR-9-transfected MG-63 cells were compared to those of control MG-63 cells. miR-9 was significantly upregulated in OS tissues and cell lines compared to the corresponding non-cancerous bone tissues (P < 0.05) and human osteoblastic cell line (P < 0.05), respectively. Upregulated miR-9 expression was also associated with increased cell proliferation (P < 0.05), migration (P < 0.05), and invasion (P < 0.05), and decreased apoptotic ability (P < 0.05). These results suggest that miR-9 may play a pivotal role in tumorigenesis and tumor progression in osteosarcoma.
authors.affiliation: Department of Orthopedics, Yantai Yuhuangding Hospital, Yantai, Shandong, China.
authors.forename: X J
authors.initials: XJ
authors.lastname: Qi
authors.affiliation: Department of Orthopedics, Yantai Yuhuangding Hospital, Yantai, Shandong, China.
authors.forename: J F
authors.initials: JF
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Yantai Yuhuangding Hospital, Yantai, Shandong, China.
authors.forename: G D
authors.initials: GD
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Yantai Yuhuangding Hospital, Yantai, Shandong, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Department of Orthopedics, Yantai Yuhuangding Hospital, Yantai, Shandong, China.
authors.forename: H L
authors.initials: HL
authors.lastname: Sun
mesh_terms: D017209:Apoptosis / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000502:physiology; D012516:Osteosarcoma / Q000235:genetics* / Q000378:metabolism*
chemical_list: C541874:MIRN92 microRNA, human; D035683:MicroRNAs