title: Effects of perfluorooctane sulfonate on the conformation and activity of bovine serum albumin.
abstract: Perfluorooctane sulfonate (PFOS) is among the most prominent contaminates in human serum and has been reported to possess potential toxicity to the human body. In this study, the effects of PFOS on the conformation and activity of bovine serum albumin (BSA) were investigated in vitro. The results indicated that the binding interaction of PFOS with BSA destroyed the tertiary and secondary structures of protein with the loss of α-helix structure and the increasing of hydrophobic microenvironment of the Trp or Tyr residues. During the thermal denaturation protein, PFOS increases the protein stability of BSA. The proportion of α-helix decreased on increasing the PFOS concentration and the microenvironment of the Trp or Tyr residues becomes more hydrophobic. The results from molecular modeling indicated that BSA had not only one possible binding site to bind with PFOS by the polar interaction, hydrogen bonds and hydrophobic forces. In addition, the BSA relative activities were decreased with the increase of PFOS concentration. Such loss of BSA activity in the presence of PFOS indicated that one of the binding sites in BSA is located in subdomain IIIA, which is in good agreement with the fluorescence spectroscopic experiments and molecular modeling results. This study offers a comprehensive picture of the interactions of PFOS with serum albumin and provides insights into the toxicological effect of perfluoroalkylated substances.
authors.affiliation: Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, People's Republic of China. Electronic address: wyqing76@126.com.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, People's Republic of China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Marine and Bio-engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, People's Republic of China.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Kang
authors.affiliation: Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, People's Republic of China. Electronic address: yctu_caojian@126.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Cao
mesh_terms: D017738:Alkanesulfonic Acids / Q000494:pharmacology*; D001665:Binding Sites; D005466:Fluorocarbons / Q000494:pharmacology*; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D062105:Molecular Docking Simulation; D011487:Protein Conformation; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D013057:Spectrum Analysis / Q000379:methods
chemical_list: D017738:Alkanesulfonic Acids; D005466:Fluorocarbons; D012710:Serum Albumin, Bovine; C076994:perfluorooctane sulfonic acid
keywords: Denaturation; Perfluorooctane sulfonate; Secondary structure; Serum albumin; Toxicological evaluation