title: Preparation of highly purified timosaponin AIII from rhizoma anemarrhenae through an enzymatic method combined with preparative liquid chromatography.
abstract: Timosaponin AIII (TAIII) exhibits extensive pharmacological activities and has been reported as a potent antitumour agent for various human cancers. In the present study, a potential industrial process for producing TAIII that involves biotransformation directly in the crude extract liquid of rhizoma anemarrhenae (RA) was developed. β-D-glycosidase was used to transform timosaponin BII (TBII) into TAIII, and monofactor experiments were conducted to optimise the enzymolysis conditions. In addition, AB-8 macroporous resin column chromatography, preparative liquid chromatography, and crystallisation technique were applied for yielding TAIII crystals with a purity > 97%. Approximately, 7 g of TAIII with a high purity of > 97% was obtained from 1 kg of RA through this five-step preparation method, which can be used to produce TAIII on a large scale.
authors.affiliation: a School of Pharmacy , Shanghai University of Traditional Chinese Medicine , Shanghai , China.
authors.forename: Lu
authors.initials: L
authors.lastname: Lu
authors.affiliation: a School of Pharmacy , Shanghai University of Traditional Chinese Medicine , Shanghai , China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Liu
authors.affiliation: b Experiment Center for Teaching and Learning , Shanghai University of Traditional Chinese Medicine , Shanghai , China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Ding
authors.affiliation: b Experiment Center for Teaching and Learning , Shanghai University of Traditional Chinese Medicine , Shanghai , China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Hou
authors.affiliation: b Experiment Center for Teaching and Learning , Shanghai University of Traditional Chinese Medicine , Shanghai , China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: b Experiment Center for Teaching and Learning , Shanghai University of Traditional Chinese Medicine , Shanghai , China.
authors.forename: Haiping
authors.initials: H
authors.lastname: Xue
authors.affiliation: b Experiment Center for Teaching and Learning , Shanghai University of Traditional Chinese Medicine , Shanghai , China.
authors.forename: Tong
authors.initials: T
authors.lastname: Zhang
mesh_terms: D031403:Anemarrhena / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000302:isolation & purification; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003460:Crystallization; D006026:Glycoside Hydrolases / Q000378:metabolism; D027343:Rhizome / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D012503:Saponins; D013256:Steroids; C543146:timosaponin AIII; C523027:timosaponin B-II; D006026:Glycoside Hydrolases
keywords: enzymolysis; preparative liquid chromatography; timosaponin AIII; β-D-glycosidase