title: Isolating Lysosomes from Rat Liver.
abstract: This protocol describes the generation of a fraction enriched in lysosomes from rat liver. The lysosomes are rapidly isolated using density-gradient centrifugation with gradient media that retain the osmolarity of the lysosomes such that they are functional and can be used in in vitro assays.
authors.affiliation: Centre for Immunology and Infection, Hull York Medical School and Department of Biology, University of York, York YO10 5DD, United Kingdom.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Pryor
mesh_terms: D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods*; D022781:Hepatocytes; D008099:Liver; D008247:Lysosomes; D051381:Rats