title: Biological carbon monoxide conversion to acetate production by mixed culture.
abstract: To utilize waste CO for mixed culture gas fermentation, carbon sources (CO, CO2) and pH were optimized in the batch system to find out the center point and boundary of response surface method (RSM) for higher acetate (HAc) production (center points: 25% CO, 40% CO2, and pH 8). The concentrations of CO and CO2, and pH had significant effects on acetate production, but the pH was the most significant on the HAc production. The optimum condition for HAc production in the gas fermentation was 20.81% CO, 41.38% CO2, 37.81% N2, and pH 7.18. The continuous gas fermentation under the optimum condition obtained 1.66g/L of cell DW, 23.6g/L HAc, 3.11g/L propionate, and 3.42g/L ethanol.
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Pohang 37673, South Korea.
authors.forename: Chul Woo
authors.initials: CW
authors.lastname: Nam
authors.affiliation: Bioenergy Research Center, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Pohang 37673, South Korea.
authors.forename: Kyung A
authors.initials: KA
authors.lastname: Jung
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Pohang 37673, South Korea; Bioenergy Research Center, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Pohang 37673, South Korea; Division of Advanced Nuclear Engineering, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Pohang 37673, South Korea. Electronic address: jmpark@postech.ac.kr.
authors.forename: Jong Moon
authors.initials: JM
authors.lastname: Park
mesh_terms: D000085:Acetates / Q000378:metabolism*; D000704:Analysis of Variance; D001419:Bacteria / Q000378:metabolism*; D061249:Batch Cell Culture Techniques / Q000379:methods*; D018533:Biomass; D002248:Carbon Monoxide / Q000378:metabolism*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration
chemical_list: D000085:Acetates; D002248:Carbon Monoxide
keywords: Acetate; Box–Behnken design; Carbon monoxide; Gas fermentation; Response surface method