title: Identification and Assessment of the Potential Allergenicity of 7S Vicilins in Olive (Olea europaea L.) Seeds.
abstract: Olive seeds, which are a raw material of interest, have been reported to contain 11S seed storage proteins (SSPs). However, the presence of SSPs such as 7S vicilins has not been studied. In this study, following a search in the olive seed transcriptome, 58 sequences corresponding to 7S vicilins were retrieved. A partial sequence was amplified by PCR from olive seed cDNA and subjected to phylogenetic analysis with other sequences. Structural analysis showed that olive 7S vicilin contains 9 α-helixes and 22 β-sheets. Additionally, 3D structural analysis displayed good superimposition with vicilin models generated from Pistacia and Sesamum. In order to assess potential allergenicity, T and B epitopes present in these proteins were identified by bioinformatic approaches. Different motifs were observed among the species, as well as some species-specific motifs. Finally, expression analysis of vicilins was carried out in protein extracts obtained from seeds of different species, including the olive. Noticeable bands were observed for all species in the 15-75 kDa MW interval, which were compatible with vicilins. The reactivity of the extracts to sera from patients allergic to nuts was also analysed. The findings with regard to the potential use of olive seed as food are discussed.
authors.affiliation: Plant Reproductive Biology Laboratory, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, 18008 Granada, Spain.
authors.forename: Jose C
authors.initials: JC
authors.lastname: Jimenez-Lopez
authors.affiliation: Plant Reproductive Biology Laboratory, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, 18008 Granada, Spain; Elayo Group, Castillo de Locubín, 23670 Jaén, Spain.
authors.forename: Adoración
authors.initials: A
authors.lastname: Zafra
authors.affiliation: Plant Reproductive Biology Laboratory, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, 18008 Granada, Spain.
authors.forename: Lucía
authors.initials: L
authors.lastname: Palanco
authors.affiliation: Allergy Service, Hospital Universitario San Cecilio, 18012 Granada, Spain.
authors.forename: José Fernando
authors.initials: JF
authors.lastname: Florido
authors.affiliation: Plant Reproductive Biology Laboratory, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, 18008 Granada, Spain.
authors.forename: Juan de Dios
authors.identifier: 0000-0002-7547-6025
authors.initials: Jde D
authors.lastname: Alché
mesh_terms: D000485:Allergens / Q000096:biosynthesis / Q000276:immunology*; D000595:Amino Acid Sequence / Q000235:genetics; D000939:Epitopes / Q000096:biosynthesis / Q000276:immunology*; D005504:Food Analysis; D018506:Gene Expression Regulation, Plant; D006801:Humans; D031658:Olea / Q000276:immunology; D055314:Seed Storage Proteins / Q000096:biosynthesis / Q000276:immunology*; D012639:Seeds / Q000276:immunology; D059467:Transcriptome / Q000276:immunology*
chemical_list: D000485:Allergens; D000939:Epitopes; D055314:Seed Storage Proteins; C015575:vicilin protein, plant