title: Lactobacillus rhamnosus RC007 intended for feed additive: immune-stimulatory properties and ameliorating effects on TNBS-induced colitis.
abstract: Lactobacillus rhamnosus RC007 is a potential probiotic bacterium that can exert beneficial effects as supplement for animal feed, by improving the immune status in healthy host, and by providing therapeutic benefits to infected/inflamed animals. The aim of the present work was to evaluate in vivo the beneficial properties of L. rhamnosus RC007, intended for animal feed, when administered to healthy and trinitro-benzene-sulfonic-acid (TNBS) colitis induced BALB/c mice. The administration of L. rhamnosus RC007 to healthy mice during 10 days increased the phagocytic activity of peritoneal macrophages and the number of immunoglobulin A+ cells in the lamina proper of the small intestine. Significant increases of monocyte chemotactic protein 1, interleukin (IL)-10 and tumour necrosis factor alpha (TNF-α) concentrations, and in the ratio between anti- and pro-inflammatory cytokines (IL-10/TNF-α) were observed in intestinal fluids after administration of bacteria. In the inflammation model, less body weight loss, macroscopic and histological damages in the large intestine were accompanied by increased IL-10/TNF-α ratio in the intestinal fluids of mice from the L. rhamnosus-TNBS group when compared to the TNBS group. In a healthy host, the oral administration of L. rhamnosus RC007 kept the gut immune system stimulated allowing a faster response to noxious stimulus. Mice that received L. rhamnosus RC007 also decreased the severity of the intestinal inflammation.
authors.affiliation: 1 Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta 36 km.601, 5800 Río Cuarto, Argentina.
authors.forename: C
authors.initials: C
authors.lastname: Dogi
authors.affiliation: 1 Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta 36 km.601, 5800 Río Cuarto, Argentina.|3 Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: G
authors.initials: G
authors.lastname: García
authors.affiliation: 2 Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145, T4000ILC San Miguel de Tucumán, Argentina.|3 Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: A
authors.initials: A
authors.lastname: De Moreno de LeBlanc
authors.affiliation: 1 Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta 36 km.601, 5800 Río Cuarto, Argentina.
authors.forename: C
authors.initials: C
authors.lastname: Greco
authors.affiliation: 1 Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta 36 km.601, 5800 Río Cuarto, Argentina.|3 Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: L
authors.initials: L
authors.lastname: Cavaglieri
mesh_terms: D000821:Animal Feed; D000818:Animals; D001835:Body Weight; D003092:Colitis / Q000139:chemically induced / Q000178:diet therapy / Q000276:immunology / Q000662:veterinary*; D016207:Cytokines / Q000378:metabolism; D005260:Female; D007422:Intestines / Q000276:immunology; D052201:Lacticaseibacillus rhamnosus; D008807:Mice, Inbred BALB C; D064307:Microbiota; D010587:Phagocytosis; D019936:Probiotics / Q000627:therapeutic use*; D014302:Trinitrobenzenesulfonic Acid
chemical_list: D016207:Cytokines; D014302:Trinitrobenzenesulfonic Acid
keywords: animal feed; gut inflammation; immune modulation; immune system; lactic acid bacteria