title: Diversity of flux distribution in central carbon metabolism of S. cerevisiae strains from diverse environments.
abstract: BACKGROUND
  S. cerevisiae has attracted considerable interest in recent years as a model for ecology and evolutionary biology, revealing a substantial genetic and phenotypic diversity. However, there is a lack of knowledge on the diversity of metabolic networks within this species.
  
  
  RESULTS
  To identify the metabolic and evolutionary constraints that shape metabolic fluxes in S. cerevisiae, we used a dedicated constraint-based model to predict the central carbon metabolism flux distribution of 43 strains from different ecological origins, grown in wine fermentation conditions. In analyzing these distributions, we observed a highly contrasted situation in flux variability, with quasi-constancy of the glycolysis and ethanol synthesis yield yet high flexibility of other fluxes, such as the pentose phosphate pathway and acetaldehyde production. Furthermore, these fluxes with large variability showed multimodal distributions that could be linked to strain origin, indicating a convergence between genetic origin and flux phenotype.
  
  
  CONCLUSIONS
  Flux variability is pathway-dependent and, for some flux, a strain origin effect can be found. These data highlight the constraints shaping the yeast operative central carbon network and provide clues for the design of strategies for strain improvement.
authors.affiliation: SPO, INRA, SupAgro, Université de Montpellier, 34060, Montpellier, France. nidelet@supagro.inra.fr.
authors.forename: Thibault
authors.initials: T
authors.lastname: Nidelet
authors.affiliation: SPO, INRA, SupAgro, Université de Montpellier, 34060, Montpellier, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Brial
authors.affiliation: SPO, INRA, SupAgro, Université de Montpellier, 34060, Montpellier, France.
authors.forename: Carole
authors.initials: C
authors.lastname: Camarasa
authors.affiliation: SPO, INRA, SupAgro, Université de Montpellier, 34060, Montpellier, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Dequin
mesh_terms: D000220:Adaptation, Biological / Q000235:genetics; D002244:Carbon / Q000378:metabolism*; D060833:Cellular Microenvironment / Q000235:genetics; D017753:Ecosystem; D019143:Evolution, Molecular; D014644:Genetic Variation; D005838:Genotype; D006019:Glycolysis / Q000235:genetics; D053858:Metabolic Networks and Pathways / Q000235:genetics; D008828:Microbiological Techniques / Q000379:methods; D010427:Pentose Phosphate Pathway / Q000235:genetics; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
chemical_list: D002244:Carbon
keywords: Diversity; Flux balance analysis; Metabolic flux; Modeling; S. cerevisiae