title: HPLC and GC-MS Analysis of Tunisian Peganum harmala Seeds Oil and Evaluation of Some Biological Activities.
abstract: In this work, we characterized by high-performance liquid chromatography, the γ-tocopherol (573.66 μg/g), and by gas chromatography-mass spectrometry, the linoleic acid (62.05%) as major components from Peganum harmala L. seeds oil and evaluated their potential in vivo acute antiinflammatory, analgesic activities, and in vitro antioxidative capacity. The 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay showed an important antioxidant activity (62.50% with an IC50 of 4.8 mg/mL). In addition, the antiinflammatory effect of the formulation cream at 20% caused a reduction in inflammation at 5 hours after carrageenan application compared with diclofenac at 1% (60.4%; 45.65%; respectively). A slight potential peripheral analgesia was noted in plantar test in treated rats with cream (20 s). Therefore, our findings demonstrate that formulation cream of P. harmala seeds oil has an interesting antiinflammatory activity with a slight peripheral analgesic effect due mainly to its richness on linoleic acid, γ-tocopherol, and polyphenols and to its important antioxidant capacity.
authors.affiliation: 1Biochemistry of Lipids and Proteins Unit, Department of Biology, Faculty of Sciences of Tunis El Manar, Tunisia; 2Laboratory of Pharmaco-toxicology, National Agency of Medicinal and Aromatic Plants, Taounate, Morocco; 3Engineering of Organometallic and Molecular Materials Laboratory (CNRST, URAC 19), Faculty of Sciences, University Sidi Mohamed Ben Abdellah, Morocco; and 4Laboratory of Chemistry, Institute of Higher Education and Lifelong Learning, Bardo, Virtual University of Tunis, Tunisia.
authors.forename: Maroua
authors.initials: M
authors.lastname: Khadhr
authors.forename: Dalila
authors.initials: D
authors.lastname: Bousta
authors.forename: El Hajaji
authors.initials: EH
authors.lastname: Hanane
authors.forename: Latifa
authors.initials: L
authors.lastname: El Mansouri
authors.forename: Smahane
authors.initials: S
authors.lastname: Boukhira
authors.forename: Mohammed
authors.initials: M
authors.lastname: Lachkar
authors.forename: Bassem
authors.initials: B
authors.lastname: Jamoussi
authors.forename: Sadok
authors.initials: S
authors.lastname: Boukhchina
mesh_terms: D000700:Analgesics / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use; D002351:Carrageenan / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003876:Dermatitis, Atopic / Q000139:chemically induced / Q000188:drug therapy*; D004195:Disease Models, Animal; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D019787:Linoleic Acid / Q000494:pharmacology; D008297:Male; D032443:Peganum / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D010938:Plant Oils / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry*; D063465:Skin Cream / Q000494:pharmacology; D024504:gamma-Tocopherol / Q000494:pharmacology
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D010936:Plant Extracts; D010938:Plant Oils; D024504:gamma-Tocopherol; D002351:Carrageenan; D019787:Linoleic Acid