title: Preparing neural stem/progenitor cells in PuraMatrix hydrogel for transplantation after brain injury in rats: A comparative methodological study.
abstract: Cultivation of neural stem/progenitor cells (NS/PCs) in PuraMatrix (PM) hydrogel is an option for stem cell transplantation. The efficacy of a novel method for placing adult rat NS/PCs in PM (injection method) was compared to encapsulation and surface plating approaches. In addition, the efficacy of injection method for transplantation of autologous NS/PCs was studied in a rat model of brain injury. NS/PCs were obtained from the subventricular zone (SVZ) and cultivated without (control) or with scaffold (three-dimensional cultures; 3D). The effect of different approaches on survival, proliferation, and differentiation of NS/PCs were investigated. In in vivo study, brain injury was induced 45 days after NS/PCs were harvested from the SVZ and phosphate buffered saline, PM, NS/PCs, or PM+NS/PCs were injected into the brain lesion. There was an increase in cell viability and proliferation after injection and surface plating of NS/PCs compared to encapsulation and neural differentiation markers were expressed seven days after culturing the cells. Using injection method, transplantation of NS/PCs cultured in PM resulted in significant reduction of lesion volume, improvement of neurological deficits, and enhancement of surviving cells. In addition, the transplanted cells could differentiate in to neurons, astrocytes, or oligodendrocytes. Our results indicate that the injection and surface plating methods enhanced cell survival and proliferation of NS/PCs and suggest the injection method as a promising approach for transplantation of NS/PCs in brain injury.
authors.affiliation: Shefa Neuroscience Research Center, Khatam-al-Anbia Hospital, Tehran, Iran; Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran; Department of Neuroscience, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Hadi
authors.initials: H
authors.lastname: Aligholi
authors.affiliation: Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Seyed Mahdi
authors.initials: SM
authors.lastname: Rezayat
authors.affiliation: Neural Stem Cell & Regenerative Neuroscience Laboratory, Department of Anatomical Sciences, School of Medicine, Shiraz, Iran; Shiraz Stem Cell Institute, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Hassan
authors.initials: H
authors.lastname: Azari
authors.affiliation: Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Shahram
authors.initials: S
authors.lastname: Ejtemaei Mehr
authors.affiliation: Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Akbari
authors.affiliation: Shefa Neuroscience Research Center, Khatam-al-Anbia Hospital, Tehran, Iran.
authors.forename: Seyed Mostafa
authors.initials: SM
authors.lastname: Modarres Mousavi
authors.affiliation: Shefa Neuroscience Research Center, Khatam-al-Anbia Hospital, Tehran, Iran; Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Attari
authors.affiliation: Shefa Neuroscience Research Center, Khatam-al-Anbia Hospital, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Alipour
authors.affiliation: Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: hassanzadeh@tums.ac.ir.
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Hassanzadeh
authors.affiliation: Shefa Neuroscience Research Center, Khatam-al-Anbia Hospital, Tehran, Iran; Department of Neuroscience, Mashhad University of Medical Sciences, Mashhad, Iran; Epilepsy Research Center, Department of Neurosurgery, and Department of Neurology, Westfälische Wilhelms-Universität Münster, Münster, Germany. Electronic address: gorjial@uni-muenster.de.
authors.forename: Ali
authors.initials: A
authors.lastname: Gorji
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000628:therapy*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D020547:Lateral Ventricles / Q000166:cytology; D008297:Male; D009044:Motor Cortex / Q000293:injuries; D058953:Neural Stem Cells / Q000502:physiology* / Q000637:transplantation* / Q000648:ultrastructure; D061251:Primary Cell Culture / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D033581:Stem Cell Transplantation / Q000379:methods*
chemical_list: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate
keywords: Autologous transplantation; Brain injury; Nanoscaffold; Neural tissue engineering; Traumatic brain lesion