title: Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus.
abstract: OBJECTIVE
  To analyze biological characteristics, malignant potential and expression of proteins associated with epithelial-mesenchymal transition in murine lung carcinoma cells transduced with interferon-beta (ifn-β) gene in baculovirus vector.
  
  
  METHODS
  The study was performed on Lewis lung carcinoma (LL) cells transduced with ifn-β gene in recombinant baculovirus vector. Biological characteristics of the LL cells were studied with the use of standard cell culture methods, cytogenetic and immunocytochemical assays.
  
  
  RESULTS
  Recombinant baculovirus-mediated transduction of LL cells with ifn-β gene resulted in significant decrease of cell growth rate and density both in complete and serum-free medium. Also, LL cells transduction with ifn-β gene significantly inhibited cell migration in vitro. Transduction of LL cells by baculovirus vector with or without ifn-β gene caused significant genotoxic effect in these cells. Furthermore, ifn-β gene transfer to lung carcinoma cells resulted in significant increase of nuclear expression of p19(ARF) (p < 0.01), p21(WAF1) (p < 0.01), cytoplasmic expression of E-cadherin (p < 0.005) and inhibition of transcription factors of epithelial-mesenchymal transition (EMT) Twist (p < 0.005) and Slug (p < 0.01) expression.
  
  
  CONCLUSIONS
  Transduction with ifn-β gene of LL cells in recombinant baculovirus resulted in acquirement of less malignant phenotype in vitro and suppressed expression of proteins associated with EMT.
authors.forename: O
authors.initials: O
authors.lastname: Lykhova
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, Ukraine.
authors.forename: O
authors.initials: O
authors.lastname: Kovalova
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, Ukraine.
authors.forename: N
authors.initials: N
authors.lastname: Bezdenezhnykh
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, Ukraine.
authors.forename: I
authors.initials: I
authors.lastname: Adamenko
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, Ukraine.
authors.forename: A
authors.initials: A
authors.lastname: Vorontsova
authors.affiliation: Institute of Molecular Biology and Genetics, NAS of Ukraine, Kyiv 03680, Ukraine.
authors.forename: L
authors.initials: L
authors.lastname: Strokovska
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, Ukraine.
authors.forename: Yu
authors.initials: Y
authors.lastname: Kudryavets
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D018827:Carcinoma, Lewis Lung / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D002465:Cell Movement; D058750:Epithelial-Mesenchymal Transition; D015972:Gene Expression Regulation, Neoplastic; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics / Q000627:therapeutic use*; D006801:Humans; D016899:Interferon-beta / Q000235:genetics*; D008168:Lung / Q000378:metabolism / Q000473:pathology*; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D014161:Transduction, Genetic
chemical_list: D016899:Interferon-beta