title: [FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIA].
abstract: OBJECTIVE
  Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
  
  
  METHODS
  Vaccine,strains: Brucella melitensis Rev-1 and Brucella abortus 19BA. Protein profiling in linear mode on Microflex "Bruker Daltonics" MALDI-TOF mass-spectrometer.
  
  
  RESULTS
  A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used.
  
  
  CONCLUSIONS
  Based on the analysis of the data obtained, use of Albimi agar as the nutrient medium for preparation of brucella culture samples for mass-spectrometric analysis was shown to be optimal.
authors.forename: D V
authors.initials: DV
authors.lastname: Ulshina
authors.forename: D A
authors.initials: DA
authors.lastname: Kovalev
authors.forename: A M
authors.initials: AM
authors.lastname: Zhirov
authors.forename: N V
authors.initials: NV
authors.lastname: Zharinova
authors.forename: A A
authors.initials: AA
authors.lastname: Khudoleev
authors.forename: O I
authors.initials: OI
authors.lastname: Kogotkova
authors.forename: V I
authors.initials: VI
authors.lastname: Efremenko
authors.forename: N I
authors.initials: NI
authors.lastname: Evchenko
authors.forename: A N
authors.initials: AN
authors.lastname: Kulichenko
mesh_terms: D000362:Agar / Q000737:chemistry / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D002003:Brucella abortus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D017347:Brucella melitensis / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D055442:Metabolome / Q000187:drug effects; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D000362:Agar