title: Poly(vinyl alcohol) Modified Porous Graphitic Carbon Stationary Phase for Hydrophilic Interaction Liquid Chromatography.
abstract: We report a poly(vinyl alcohol) (PVA)-coated porous graphitic carbon (PGC, Hypercarb) packing as a novel stationary phase for hydrophilic interaction liquid chromatography (HILIC). The exterior and the pores of the PGC particles are coated with a thin layer of PVA by soaking the particles in a PVA solution, filtering, and thermally cross-linking the PVA. Such PVA coated PGC particles (5.7 μm diameter), hereinafter called PVA-PGC are stable at least through pH 1.0-12.7, can be made in <2 h, and exhibit different selectivity relative to six commercial HILIC phases and bare PGC. To our knowledge, this is the first fully pH-stable, completely neutral HILIC phase. Excellent efficiency stable is observed for polar analytes (∼70 000 and 118 000 plates/m for cytosine and resorcinol, respectively). Retention closely resembles standard HILIC behavior. Other substances can also be easily incorporated in the PVA layer; an anion exchange column can be readily made by incorporating diallyldimethylammonium chloride in the PVA coating solution. The ease of preparation without the requirement of synthetic skills or paraphernalia and the possibility of incorporating a variety of modifiers makes this a particularly versatile approach.
authors.affiliation: School of Pharmacy, East China University of Science and Technology , Shanghai 200237, China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Hou
authors.affiliation: School of Pharmacy, East China University of Science and Technology , Shanghai 200237, China.
authors.forename: Feifang
authors.initials: F
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, East China University of Science and Technology , Shanghai 200237, China.
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
authors.affiliation: School of Pharmacy, East China University of Science and Technology , Shanghai 200237, China.
authors.forename: Bingcheng
authors.initials: B
authors.lastname: Yang
authors.affiliation: Thermo Fisher Scientific , 445 Lakeside Drive, Sunnyvale, California 94087, United States.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Chemistry and Biochemistry, University of Texas at Arlington , Arlington, Texas 76019-0065, United States.
authors.forename: Purnendu K
authors.initials: PK
authors.lastname: Dasgupta