title: Inhibition of Listeria monocytogenes on cooked cured chicken breasts by acidified coating containing allyl isothiocyanate or deodorized Oriental mustard extract.
abstract: Ready-to-eat meats are considered foods at high risk to cause life-threatening Listeria monocytogenes infections. This study screened 5 L. monocytogenes strains for their ability to hydrolyze sinigrin (a glucosinolate in Oriental mustard), which formed allyl isothiocyanate (AITC) and reduced L. monocytogenes viability on inoculated vacuum-packed, cooked, cured roast chicken slices at 4 °C. Tests involved incorporation of 25-50 μl/g AITC directly or 100-250 mg/g Oriental mustard extract in 0.5% (w/v) κ-carrageenan/2% (w/v) chitosan-based coatings prepared using 1.5% malic or acetic acid. L. monocytogenes strains hydrolyzed 33.6%-48.4% pure sinigrin in MH broth by 21 d at 25 °C. Acidified κ-carrageenan/chitosan coatings containing 25-50 μl/g AITC or 100-250 mg/g mustard reduced the viability of L. monocytogenes and aerobic bacteria on cooked, cured roast chicken slices by 4.1 to >7.0 log10 CFU/g compared to uncoated chicken stored at 4 °C for 70 d. Coatings containing malic acid were significantly more antimicrobial than those with acetic acid. During storage for 70 d, acidified κ-carrageenan/chitosan coatings containing 25-50 μl/g AITC or 250 mg/g mustard extract reduced lactic acid bacteria (LAB) numbers 3.8 to 5.4 log10 CFU/g on chicken slices compared to uncoated samples. Acidified κ-carrageenan/chitosan-based coatings containing either AITC or Oriental mustard extract at the concentrations tested had the ability to control L. monocytogenes viability and delay growth of potential spoilage bacteria on refrigerated, vacuum-packed cured roast chicken.
authors.affiliation: Department of Food Science, Faculty of Agriculture and Food Science, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada.
authors.forename: Amin N
authors.initials: AN
authors.lastname: Olaimat
authors.affiliation: Department of Food Science, Faculty of Agriculture and Food Science, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada. Electronic address: rick_holley@umanitoba.ca.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Holley
mesh_terms: D000818:Animals; D002645:Chickens; D003296:Cooking; D005506:Food Contamination / Q000517:prevention & control; D005519:Food Preservation / Q000295:instrumentation / Q000379:methods*; D005520:Food Preservatives / Q000737:chemistry / Q000494:pharmacology*; D017879:Isothiocyanates / Q000494:pharmacology*; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development; D008460:Meat / Q000032:analysis / Q000382:microbiology*; D009149:Mustard Plant / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D005520:Food Preservatives; D017879:Isothiocyanates; D010936:Plant Extracts; C004471:allyl isothiocyanate
keywords: Acetic acid; Allyl isothiocyanate; Antimicrobial coating; L. monocytogenes; Malic acid; Oriental mustard; Ready-to-eat meat