title: A Fluorescence Turn-On Probe for Thiols with a Tunable Dynamic Range.
abstract: We designed and synthesized a novel probe Cou-Rho-SA-Cu (II) for detection of thiols based on a coumarin-rhodamine FRET system. The compound Cou-Rho-SA exhibited strong blue fluorescence as the rhodamine moiety of the compound Cou-Rho-SA was in a ring-closing form and no FRET process occurred. The probe Cou-Rho-SA-Cu (II) was prepared by addition of Cu(2+) to the solution of Cou-Rho-SA. In the absence of GSH, the complex Cou-Rho-SA-Cu (II) showed almost no fluorescence owing to the FRET process and the paramagnetic nature of Cu(2+) . However, upon addition of GSH, the rhodamine moiety of the probe Cou-Rho-SA-Cu (II) recovered to its ring-closing form, and the FRET process was prohibited to show the blue fluorescence again. Moreover, via tuning the Cu(2+) concentrations, the probe could detect the different levels of GSH.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan, 410082, People's Republic of China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan, 410082, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan, 410082, People's Republic of China. weiyinglin2013@163.com.|Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Biological Science and Technology, University of Jinan, Jinan, Shandong, 250022, People's Republic of China. weiyinglin2013@163.com.
authors.forename: Weiying
authors.initials: W
authors.lastname: Lin
keywords: Cu2+ complex; Dynamic range; FRET; Fluorescent probe; Thiols