title: The beneficial effects of a gas-permeable flask for expansion of Tumor-Infiltrating lymphocytes as reflected in their mitochondrial function and respiration capacity.
abstract: Adoptive transfer of autologousex vivoexpanded tumor-infiltrating lymphocytes (TIL) is a highly successful cell therapy approach in the treatment of late-stage melanoma. Notwithstanding the success of this therapy, only very few centers worldwide can provide it. To make this therapy broadly available, one of the major obstacles to overcome is the complexity of culturing the TIL. Recently, major efforts have been deployed to resolve this issue. The use of the Gas-permeable flask (G-Rex) during the REP has been one application that has facilitated this process. Here we show that the use of this new device is able to rescue poor TIL growth and maintain clonal diversity while supporting an improved mitochondrial function.
authors.affiliation: Department of Melanoma Medical Oncology; The University of Texas MD Anderson Cancer Center (MDACC) ; Houston, TX USA.
authors.forename: Marie-Andrée
authors.initials: MA
authors.lastname: Forget
authors.affiliation: Department of Melanoma Medical Oncology; The University of Texas MD Anderson Cancer Center (MDACC) ; Houston, TX USA.
authors.forename: Cara
authors.initials: C
authors.lastname: Haymaker
authors.affiliation: Department of Systems Biology; The University of Texas MDACC ; Houston, TX USA.
authors.forename: Jennifer B
authors.initials: JB
authors.lastname: Dennison
authors.affiliation: Department of Melanoma Medical Oncology; The University of Texas MD Anderson Cancer Center (MDACC) ; Houston, TX USA.
authors.forename: Christopher
authors.initials: C
authors.lastname: Toth
authors.affiliation: Division of Pediatrics; The University of Texas MDACC ; Houston, TX USA.
authors.forename: Sourindra
authors.initials: S
authors.lastname: Maiti
authors.affiliation: Department of Melanoma Medical Oncology; The University of Texas MD Anderson Cancer Center (MDACC) ; Houston, TX USA.
authors.forename: Orenthial J
authors.initials: OJ
authors.lastname: Fulbright
authors.affiliation: Division of Pediatrics; The University of Texas MDACC ; Houston, TX USA.
authors.forename: Laurence J N
authors.initials: LJ
authors.lastname: Cooper
authors.affiliation: Department of Melanoma Medical Oncology; The University of Texas MD Anderson Cancer Center (MDACC) ; Houston, TX USA.
authors.forename: Patrick
authors.initials: P
authors.lastname: Hwu
authors.affiliation: Department of Melanoma Medical Oncology; The University of Texas MD Anderson Cancer Center (MDACC); Houston, TX USA; Lion Biotechnologies; Tampa, FL USA; Department of Immunology; H. Lee Moffitt Cancer Center; Tampa, FL USA.
authors.forename: Laszlo G
authors.initials: LG
authors.lastname: Radvanyi
authors.affiliation: Department of Melanoma Medical Oncology; The University of Texas MD Anderson Cancer Center (MDACC) ; Houston, TX USA.
authors.forename: Chantale
authors.initials: C
authors.lastname: Bernatchez
keywords: TIL ACT; gas-permeable membrane; mitochondrial function; oxidative phenotype; tumor-infiltrating lymphocytes