title: Reversible Fluorescent Probe for Selective Detection and Cell Imaging of Oxidative Stress Indicator Bisulfite.
abstract: In this paper, we report a benzothiazole-functionalized cyanine fluorescence probe and demonstrate that it is selectively reactive to bisulfite, an intermediate indicator for oxidative stress. The selective reaction can be monitored by distinct ratiometric fluorescence variation favorable for cell imaging and visualization. The original probe can be regenerated in high yield through the elimination of bisulfite from the product by peroxides such as hydrogen peroxide, accompanied by fluorescence turning on at 590 nm, showing a potential application for the detection of peroxides. We successfully applied this probe for fluorescence imaging of bisulfite in cancer cells (MCF-7) treated with bisulfite and hydrogen peroxide as well as a selective detection limit of 0.34 μM bisulfite in aqueous solution.
authors.affiliation: Institute of Intelligent Machines, Chinese Academy of Sciences , Hefei, Anhui 230031, China.|Department of Materials Science and Engineering, University of Science and Technology of China , Hefei, Anhui 230026, China.
authors.forename: Yajiao
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.
authors.forename: Lingmei
authors.initials: L
authors.lastname: Guan
authors.affiliation: Institute of Intelligent Machines, Chinese Academy of Sciences , Hefei, Anhui 230031, China.|Department of Materials Science and Engineering, University of Science and Technology of China , Hefei, Anhui 230026, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Yu
authors.affiliation: Institute of Intelligent Machines, Chinese Academy of Sciences , Hefei, Anhui 230031, China.|Department of Materials Science and Engineering, University of Science and Technology of China , Hefei, Anhui 230026, China.
authors.forename: Yehan
authors.initials: Y
authors.lastname: Yan
authors.affiliation: State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.
authors.forename: Libo
authors.initials: L
authors.lastname: Du
authors.affiliation: State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Intelligent Machines, Chinese Academy of Sciences , Hefei, Anhui 230031, China.|Department of Materials Science and Engineering, University of Science and Technology of China , Hefei, Anhui 230026, China.
authors.forename: Mingtai
authors.initials: M
authors.lastname: Sun
authors.affiliation: Food Science and Technology Programme, Department of Chemistry, National University of Singapore , 3 Science Drive 3, Singapore 117543, Republic of Singapore.
authors.forename: Dejian
authors.initials: D
authors.lastname: Huang
authors.affiliation: Institute of Intelligent Machines, Chinese Academy of Sciences , Hefei, Anhui 230031, China.|Department of Materials Science and Engineering, University of Science and Technology of China , Hefei, Anhui 230026, China.
authors.forename: Suhua
authors.initials: S
authors.lastname: Wang
mesh_terms: D002232:Carbocyanines / Q000138:chemical synthesis / Q000737:chemistry*; D002470:Cell Survival; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis / Q000737:chemistry; D061986:MCF-7 Cells; D015394:Molecular Structure; D018384:Oxidative Stress; D013447:Sulfites / Q000032:analysis* / Q000737:chemistry; D013458:Sulfur Dioxide / Q000032:analysis
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; D013447:Sulfites; D013458:Sulfur Dioxide; D006861:Hydrogen Peroxide; C042345:hydrogen sulfite