title: Metabolic profiling of Shu-Yu capsule in rat serum based on metabolic fingerprinting analysis using HPLC-ESI-MSn.
abstract: The Chinese herbal formula, Shu-Yu capsule (SYC), has been successfully used to treat depression-like disorders in clinical settings. To rapidly identify the chemical constituents of SYC and its metabolites in rat serum, a simple and sensitive liquid chromatography-tandem mass spectrometry method was established in the present study. By comparing the retention times, MS and MSn spectra data in the literature and reference standards, a total of 73 compounds were identified from SYC. In rat serum, 62 components, including 13 prototype compounds and 49 metabolites were identified. Of these components, 14 metabolites were confirmed as novel metabolites of SYC. The results of the present study indicated that certain ﬂavonoid glycosides and monoterpene glycosides were absorbed directly. Glucuronidation and sulfation were identified as the predominant metabolic pathways of the components in SYC. In addition, certain phase I reactions, including hydrolysis, demethylation and hydroxylation occurred in the rats. These results provide scientific evidence, which support further investigations of the pharmacology and mechanism of SYC.
authors.affiliation: Hospital Pharmacy, Fengtai Maternal and Children's Health Hospital of Beijing, Beijing 100069, P.R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs and Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Peking University, Beijing 100101, P.R. China.
authors.forename: You-Bo
authors.initials: YB
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology and Toxicology, Shandong Institute for Food and Drug Control, Jinan, Shandong 250012, P.R. China.
authors.forename: Xia
authors.initials: X
authors.lastname: Wei
authors.affiliation: Institute of Traditional Chinese Medicine Theory, School of Basic Medicine, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 100044, P.R. China.
authors.forename: Chun-Hong
authors.initials: CH
authors.lastname: Song
authors.affiliation: Institute of Traditional Chinese Medicine Theory, School of Basic Medicine, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 100044, P.R. China.
authors.forename: Ming-Qi
authors.initials: MQ
authors.lastname: Qiao
authors.affiliation: Institute of Traditional Chinese Medicine Theory, School of Basic Medicine, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 100044, P.R. China.
authors.forename: Hui-Yun
authors.initials: HY
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics* / Q000494:pharmacology*; D008297:Male; D055442:Metabolome / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004365:Drugs, Chinese Herbal