title: Measurement of PTEN by Flow Cytometry.
abstract: Recent advancements have driven the development of smaller footprint, less expensive, and user-friendly flow cytometers introducing the technology to more users.Flow cytometry is an established tool for multiparametric analysis of various important cellular characteristics. Fluorescent dyes or fluorophore-conjugated antibodies allow for measurement of protein expression, identification of cell populations, or DNA content analysis. This is combined with analysis of light-scattering detection to determine cell size and complexity to allow for the study of complex cell samples, such as whole blood. Through antibody staining for a variety of surface markers as well as intracellular proteins we can also elucidate intracellular signaling, and phosphor-signaling, on a single-cell basis.Here we describe the application of flow cytometry analysis to the tumor suppressor PTEN in various cancer cell lines and a mouse model.
authors.affiliation: Department of Pharmacology and Toxicology, University of Toronto, Toronto, Canada. j.woolley@utoronto.ca.
authors.forename: John F
authors.initials: JF
authors.lastname: Woolley
authors.affiliation: Department of Pharmacology and Toxicology, University of Toronto, PrincessMargaret Cancer Centre, Room 4211, Medical SciencesBuilding, 1 King's College Circle, Toronto, M5S 1A8, ON, Canada.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Salmena
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D009369:Neoplasms / Q000235:genetics; D051059:PTEN Phosphohydrolase / Q000032:analysis* / Q000235:genetics
chemical_list: D051059:PTEN Phosphohydrolase
keywords: CD45; Flow cytometry; Mouse model; PTEN; Tumor suppressor