title: Grifola frondosa Glycoprotein GFG-3a Arrests S phase, Alters Proteome, and Induces Apoptosis in Human Gastric Cancer Cells.
abstract: GFG-3a is a novel glycoprotein previously purified from the fermented mycelia of Grifola frondosa with novel sugar compositions and protein sequencing. The present study aims to investigate its effects on the cell cycle, differential proteins expression, and apoptosis of human gastric cancer SGC-7901 cells. Our findings revealed that GFG-3a induced the cell apoptosis and arrested cell cycle at S phase. GFG-3a treatment resulted in the differential expression of 21 proteins in SGC-7901 cells by upregulating 10 proteins including RBBP4 associated with cell cycle arrest and downregulating 11 proteins including RUVBL1, NPM, HSP90AB1, and GRP78 involved in apoptosis and stress response. qRT-PCR and Western blot analysis also suggested that GFG-3a could increase the expressions of Caspase-8/-3, p53, Bax, and Bad while decrease the expressions of Bcl2, Bcl-xl, PI3K, and Akt1. These results indicated that the stress response, p53-dependent mitochondrial-mediated, Caspase-8/-3-dependent, and PI3k/Akt pathways were involved in the GFG-3a-induced apoptosis process in SGC-7901 cells. These findings might provide a basis to prevent or treat human gastric cancer with GFG-3a and understand the tumor-inhibitory molecular mechanisms of mushroom glycoproteins.
authors.affiliation: a School of Food and Biological Engineering, Jiangsu University , Zhenjiang , China.|b Jiangxi Provincial Engineering and Technology Center for Food Additives Bio-production , Dexing , China.
authors.forename: Fengjie
authors.initials: F
authors.lastname: Cui
authors.affiliation: a School of Food and Biological Engineering, Jiangsu University , Zhenjiang , China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Zan
authors.affiliation: a School of Food and Biological Engineering, Jiangsu University , Zhenjiang , China.
authors.forename: Yunhong
authors.initials: Y
authors.lastname: Li
authors.affiliation: a School of Food and Biological Engineering, Jiangsu University , Zhenjiang , China.|b Jiangxi Provincial Engineering and Technology Center for Food Additives Bio-production , Dexing , China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Sun
authors.affiliation: c National Engineering Research Center of Edible Fungi, Shanghai Academy of Agricultural Sciences , Shanghai , China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: d State Key Lab Breeding Base for Quality and Safety of Agro-products, MOA Key Lab for Pesticide Residue Detection, Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences , Hangzhou , China.|e School of Civil Engineering and Architecture, Zhejiang University of Science and Technology , Hangzhou , China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Ping
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D000091342:Endoplasmic Reticulum Chaperone BiP; D005656:Fungal Proteins / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006023:Glycoproteins / Q000494:pharmacology*; D048691:Grifola / Q000737:chemistry*; D006801:Humans; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D059807:S Phase Cell Cycle Checkpoints / Q000187:drug effects; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; D000091342:Endoplasmic Reticulum Chaperone BiP; D005656:Fungal Proteins; D006023:Glycoproteins; C000717247:HSPA5 protein, human; D011506:Proteins