title: Indigofera oblongifolia Ameliorates Lead Acetate-Induced Testicular Oxidative Damage and Apoptosis in a Rat Model.
abstract: Lead (Pb) enhances the production of reactive oxygen species and depletes the antioxidant molecules that cause tissue damage. In the current study, we investigated the protective effect of Indigofera oblongifolia (hasr in Arabic) against lead acetate-induced reproductive toxicity in rats. Exposure of rats to lead acetate (PbAc; 20 mg/kg body weight; intraperitoneal injection) induced a significant change in both of body weight loss and the relative testis weight. Furthermore, a significant increase in lipid peroxidation and nitric oxide and a marked depletion of glutathione were evident in the testis of the PbAc group compared to the control group. Also, PbAc significantly reduced the activity of antioxidant enzymes. Pre-administration of I. oblongifolia leaves extract (IOLE; 100 mg/kg body weight) to the PbAc-treated rats restored most of the parameters mentioned above to near-normal levels. Additionally, pretreatment of animals with IOLE accompanied with a significant decrease in the toxic effects of PbAc as shown by caspase-3 and Bax expressions and prevented the histological injury in the testis. On the basis of the above results, I. oblongifolia appeared to be a promising agent for protection against lead-induced oxidative damage and apoptosis in the testis of rat.
authors.affiliation: Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.|Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt.
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Dkhil
authors.affiliation: Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt. aest1977@hotmail.com.
authors.forename: Ahmed E Abdel
authors.initials: AE
authors.lastname: Moneim
authors.affiliation: Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Saleh
authors.initials: S
authors.lastname: Al-Quraishy
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000096:biosynthesis; D005786:Gene Expression Regulation / Q000187:drug effects; D029883:Indigofera / Q000737:chemistry*; D008297:Male; D009942:Organometallic Compounds / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000378:metabolism* / Q000473:pathology; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis
chemical_list: C494828:Bax protein, rat; D009942:Organometallic Compounds; D010936:Plant Extracts; D051028:bcl-2-Associated X Protein; C505410:Casp3 protein, rat; D053148:Caspase 3; C008261:lead acetate
keywords: Apoptosis; Indigofera oblongifolia; Lead acetate; Oxidative stress; Testis