title: Data on hepatic lipolysis, adipose triglyceride lipase, and hormone-sensitive lipase in fasted and non-fasted C57BL/6J female mice.
abstract: Liver homogenates produced from fasted and non-fasted C57BL/6J female mice were assayed for total lipolytic activity measured as hydrolysis of [9,10-(3)H(N)]-triolein into [(3)H] free fatty acids (FFA). Liver homogenates were also used for immunoblotting to determine levels of the lipolytic enzymes adipose-triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL), as well as site specific phosphorylation at the 14-3-3 binding site of ATGL and the serine 565 and serine 660 sites of HSL. Significantly higher triolein hydrolysis activity was observed in fasted liver samples, as well as a significant increase in total ATGL and a significant decrease in HSL phosphorylation at the S565 site.
authors.affiliation: University of Waterloo, Department of Kinesiology, Faculty of Applied Health Sciences, 200 University Avenue W., BMH1110, Waterloo, Ontario, Canada N2L 3G1.
authors.forename: Phillip M
authors.initials: PM
authors.lastname: Marvyn
authors.affiliation: University of Waterloo, Department of Kinesiology, Faculty of Applied Health Sciences, 200 University Avenue W., BMH1110, Waterloo, Ontario, Canada N2L 3G1.
authors.forename: Emily B
authors.initials: EB
authors.lastname: Mardian
authors.affiliation: University of Waterloo, Department of Kinesiology, Faculty of Applied Health Sciences, 200 University Avenue W., BMH1110, Waterloo, Ontario, Canada N2L 3G1.
authors.forename: Ryan M
authors.initials: RM
authors.lastname: Bradley
authors.affiliation: University of Waterloo, Department of Kinesiology, Faculty of Applied Health Sciences, 200 University Avenue W., BMH1110, Waterloo, Ontario, Canada N2L 3G1.
authors.forename: Kristin
authors.initials: K
authors.lastname: A Marks
authors.affiliation: University of Waterloo, Department of Kinesiology, Faculty of Applied Health Sciences, 200 University Avenue W., BMH1110, Waterloo, Ontario, Canada N2L 3G1.
authors.forename: Robin E
authors.initials: RE
authors.lastname: Duncan