title: Muse Cells, a New Type of Pluripotent Stem Cell Derived from Human Fibroblasts.
abstract: A new type of mesenchymal stem cells (MSCs) that expresses stage-specific embryonic antigen 3 (SSEA-3) and the mesenchymal cell marker CD105 are known as multilineage-differentiating stress-enduring (Muse) cells. Studies have shown that stem cells in suspension cultures are more likely to generate embryoid body-like stem cell spheres and maintain an undifferentiated phenotype and pluripotency. We separated Muse cells derived from human dermal fibroblasts by long-term trypsin incubation (LTT) through suspension cultures in methylcellulose. The Muse cells obtained expressed several pluripotency markers, including Nanog, Oct4, Sox2, and SSEA-3, and could differentiate in vitro into cells of the three germ layers, such as hepatocytes (endodermal), neural cells (ectodermal) and adipocytes, and osteocytes (mesodermal cells). These cells showed a low level of DNA methylation and a high nucleo-cytoplasmic ratio. Our study provides an innovative and exciting platform for exploring the potential cell-based therapy of various human diseases using Muse cells as well as their great possibility for regenerative medicine.
authors.affiliation: 1 Department of Dermatology, The Third Affiliated Hospital of Suzhou University , Changzhou, 213003, China .
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.affiliation: 1 Department of Dermatology, The Third Affiliated Hospital of Suzhou University , Changzhou, 213003, China .
authors.forename: Ru-zhi
authors.initials: RZ
authors.lastname: Zhang
authors.affiliation: 1 Department of Dermatology, The Third Affiliated Hospital of Suzhou University , Changzhou, 213003, China .
authors.forename: Di
authors.initials: D
authors.lastname: Li
authors.affiliation: 2 Department of Dermatology, The First Affiliated Hospital of Bengbu Medical College , Anhui, 213003, China .
authors.forename: Sai
authors.initials: S
authors.lastname: Cheng
authors.affiliation: 1 Department of Dermatology, The Third Affiliated Hospital of Suzhou University , Changzhou, 213003, China .
authors.forename: Yu-hua
authors.initials: YH
authors.lastname: Yang
authors.affiliation: 1 Department of Dermatology, The Third Affiliated Hospital of Suzhou University , Changzhou, 213003, China .
authors.forename: Ting
authors.initials: T
authors.lastname: Tian
authors.affiliation: 2 Department of Dermatology, The First Affiliated Hospital of Bengbu Medical College , Anhui, 213003, China .
authors.forename: Xiao-ru
authors.initials: XR
authors.lastname: Pan
mesh_terms: D000943:Antigens, Differentiation / Q000096:biosynthesis; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000096:biosynthesis; D065150:Cellular Reprogramming; D000071063:Endoglin / Q000096:biosynthesis; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D055746:Stage-Specific Embryonic Antigens / Q000096:biosynthesis
chemical_list: D000943:Antigens, Differentiation; D015295:Antigens, Tumor-Associated, Carbohydrate; C064206:ENG protein, human; D000071063:Endoglin; D055746:Stage-Specific Embryonic Antigens; C052924:stage-specific embryonic antigen-3