title: Simultaneous and rapid determination of deoxynivalenol and its acetylate derivatives in wheat flour and rice by ultra high performance liquid chromatography with photo diode array detection.
abstract: A simple and reliable method of ultra high performance liquid chromatography coupled with photo-diode array detection has been proposed for the simultaneous determination of deoxynivalenol and its acetylated derivatives in wheat flour and rice, especially focusing on the optimization of sample extraction, cleanup, and chromatographic separation conditions. Sample pretreatment consisted of a first step using a quick, easy, cheap, effective, rugged, and safe based extraction procedure and a subsequent cleanup step based on solid-phase extraction. The method was extensively validated in wheat flour and rice, obtaining satisfactory analytical performance with good linearity (R(2) ≥ 0.999), acceptable recoveries (80.0-104.4%), and repeatability (RSDs 1.3-10.7%). The limits of detection (21.7-57.4 μg/kg) and quantitation (72.3-191.4 μg/kg) for deoxynivalenols were lower than those usually permitted by various countries' legislation in these food matrices. The method was applied to 34 wheat and rice samples. The results were further compared with results of ultra high performance liquid chromatography with electrospray ionization tandem mass spectrometry.
authors.affiliation: Lab of Physicochemical Research, Department of Physicochemical & Toxicology, Zhejiang Provincial Centre for Disease Control and Prevention, Zhejiang, China.
authors.forename: Jiao-Jiao
authors.initials: JJ
authors.lastname: Xu
authors.affiliation: College of Chemical Engineering, Zhejiang University of Technology, Zhejiang, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Lab of Physicochemical Research, Department of Physicochemical & Toxicology, Zhejiang Provincial Centre for Disease Control and Prevention, Zhejiang, China.
authors.forename: Bai-Fen
authors.initials: BF
authors.lastname: Huang
authors.affiliation: Lab of Physicochemical Research, Department of Physicochemical & Toxicology, Zhejiang Provincial Centre for Disease Control and Prevention, Zhejiang, China.
authors.forename: Zeng-Xuan
authors.initials: ZX
authors.lastname: Cai
authors.affiliation: Lab of Physicochemical Research, Department of Physicochemical & Toxicology, Zhejiang Provincial Centre for Disease Control and Prevention, Zhejiang, China.
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Xu
authors.affiliation: Lab of Physicochemical Research, Department of Physicochemical & Toxicology, Zhejiang Provincial Centre for Disease Control and Prevention, Zhejiang, China.|College of Chemical Engineering, Zhejiang University of Technology, Zhejiang, China.
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Ren
mesh_terms: D000107:Acetylation; D002851:Chromatography, High Pressure Liquid; D005433:Flour / Q000032:analysis*; D012275:Oryza / Q000737:chemistry*; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization; D014255:Trichothecenes / Q000032:analysis*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D014255:Trichothecenes; C007262:deoxynivalenol
keywords: Acetyl-deoxynivalenol; Deoxynivalenol; Solid-phase extraction; Ultra high performance liquid chromatography