title: ANALYSIS OF EXPRESSED SEQUENCE TAGS FROM THE MARINE MICROALGA NANNOCHLOROPSIS OCULATA (EUSTIGMATOPHYCEAE)(1).
abstract: Nannochloropsis oculata (Droop) D. J. Hibberd (Eustigmatophyceae), a marine eukaryotic unicellular alga, is widely used in mariculture as live feed. It is considered to be of high nutritional value owing to its high content of proteins; polyunsaturated fatty acids (PUFAs), especially eicosapentaenoic acid (EPA, C20:5n3); and diverse pigments. Previous studies of this microalga focused on its taxonomy, culture, and biochemistry, but little is known at the molecular level. Establishing a molecular base is vital to understand the biological processes of this alga. Therefore, we constructed a cDNA library using algal cells grown at exponential growth phase and carried out expressed sequence tag (EST) analysis. A total of 1,960 nonredundant sequences (NRSs) were generated for N. oculata clone CS-179. Only 32.5% of NRSs showed significant similarity (E < 1e-04) to proteins registered in the GenBank nonredundant protein database. The KOG (clusters of euKaryotic Orthologous Groups) profile database returned significant hits for 490 NRSs. Analysis revealed that a large proportion of NRSs could be unique to this microalga.
authors.affiliation: Ministry of Education Key Laboratory of Mariculture, Ocean University of China, Qingdao 266003, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaMinistry of Education Key Laboratory of Marine Drugs, Ocean University of China, Qingdao 266003, ChinaMinistry of Education Key Laboratory of Bioinformatics, Tsinghua University, Beijing 100084, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Shi
authors.affiliation: Ministry of Education Key Laboratory of Mariculture, Ocean University of China, Qingdao 266003, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaMinistry of Education Key Laboratory of Marine Drugs, Ocean University of China, Qingdao 266003, ChinaMinistry of Education Key Laboratory of Bioinformatics, Tsinghua University, Beijing 100084, China.
authors.forename: Kehou
authors.initials: K
authors.lastname: Pan
authors.affiliation: Ministry of Education Key Laboratory of Mariculture, Ocean University of China, Qingdao 266003, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaMinistry of Education Key Laboratory of Marine Drugs, Ocean University of China, Qingdao 266003, ChinaMinistry of Education Key Laboratory of Bioinformatics, Tsinghua University, Beijing 100084, China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Yu
authors.affiliation: Ministry of Education Key Laboratory of Mariculture, Ocean University of China, Qingdao 266003, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaMinistry of Education Key Laboratory of Marine Drugs, Ocean University of China, Qingdao 266003, ChinaMinistry of Education Key Laboratory of Bioinformatics, Tsinghua University, Beijing 100084, China.
authors.forename: Baohua
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Ministry of Education Key Laboratory of Mariculture, Ocean University of China, Qingdao 266003, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaMinistry of Education Key Laboratory of Marine Drugs, Ocean University of China, Qingdao 266003, ChinaMinistry of Education Key Laboratory of Bioinformatics, Tsinghua University, Beijing 100084, China.
authors.forename: Guanpin
authors.initials: G
authors.lastname: Yang
authors.affiliation: Ministry of Education Key Laboratory of Mariculture, Ocean University of China, Qingdao 266003, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaMinistry of Education Key Laboratory of Marine Drugs, Ocean University of China, Qingdao 266003, ChinaMinistry of Education Key Laboratory of Bioinformatics, Tsinghua University, Beijing 100084, China.
authors.forename: Wengong
authors.initials: W
authors.lastname: Yu
authors.affiliation: Ministry of Education Key Laboratory of Mariculture, Ocean University of China, Qingdao 266003, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaMinistry of Education Key Laboratory of Marine Drugs, Ocean University of China, Qingdao 266003, ChinaMinistry of Education Key Laboratory of Bioinformatics, Tsinghua University, Beijing 100084, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Zhang
keywords: Eustigmatophyceae; Nannochloropsis oculata; expressed sequence tag; genomics; lipid metabolism