title: Generation and Cryopreservation of Clinical Grade Wilms' Tumor 1 mRNA-Loaded Dendritic Cell Vaccines for Cancer Immunotherapy.
abstract: First described in the 1970s, dendritic cells (DC) are currently subjects of intense investigation to exploit their unique antigen-presenting and immunoregulatory capacities. In cancer, DC show promise to elicit or amplify immune responses directed against cancer cells by activating natural killer (NK) cells and tumor antigen-specific T cells. Wilms' tumor 1 (WT1) protein is a tumor-associated antigen that is expressed in a majority of cancer types and has been designated as an antigen of major interest to be targeted in clinical cancer immunotherapy trials. In this chapter, we describe the generation, cryopreservation, and thawing of clinical grade autologous monocyte-derived DC vaccines that are loaded with WT1 by messenger RNA (mRNA) electroporation. This in-house-developed transfection method gives rise to presentation of multiple antigen epitopes and can be used for all patients without restriction of human leukocyte antigen (HLA) type.
authors.affiliation: Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute, University of Antwerp, Campus Drie Eiken, Universiteitsplein 1, D.T.431, Wilrijk, Antwerp, 2610, Belgium. evelien.smits@uza.be.|Center for Cell Therapy and Regenerative Medicine, Antwerp University Hospital, Edegem, Belgium. evelien.smits@uza.be.
authors.forename: Evelien L J M
authors.initials: EL
authors.lastname: Smits
authors.affiliation: Center for Cell Therapy and Regenerative Medicine, Antwerp University Hospital, Edegem, Belgium.
authors.forename: Barbara
authors.initials: B
authors.lastname: Stein
authors.affiliation: Center for Cell Therapy and Regenerative Medicine, Antwerp University Hospital, Edegem, Belgium.
authors.forename: Griet
authors.initials: G
authors.lastname: Nijs
authors.affiliation: Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute, University of Antwerp, Campus Drie Eiken, Universiteitsplein 1, D.T.431, Wilrijk, Antwerp, 2610, Belgium.|Center for Cell Therapy and Regenerative Medicine, Antwerp University Hospital, Antwerp, Belgium.
authors.forename: Eva
authors.initials: E
authors.lastname: Lion
authors.affiliation: Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute, University of Antwerp, Campus Drie Eiken, Universiteitsplein 1, D.T.431, Wilrijk, Antwerp, 2610, Belgium.
authors.forename: Viggo F
authors.initials: VF
authors.lastname: Van Tendeloo
authors.affiliation: Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute, University of Antwerp, Campus Drie Eiken, Universiteitsplein 1, D.T.431, Wilrijk, Antwerp, 2610, Belgium.|Center for Cell Therapy and Regenerative Medicine, Antwerp University Hospital, Edegem, Belgium.
authors.forename: Yannick
authors.initials: Y
authors.lastname: Willemen
authors.affiliation: Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute, University of Antwerp, Campus Drie Eiken, Universiteitsplein 1, D.T.431, Wilrijk, Antwerp, 2610, Belgium.|Center for Cell Therapy and Regenerative Medicine, Antwerp University Hospital, Edegem, Belgium.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Anguille
authors.affiliation: Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute, University of Antwerp, Campus Drie Eiken, Universiteitsplein 1, D.T.431, Wilrijk, Antwerp, 2610, Belgium.|Center for Cell Therapy and Regenerative Medicine, Antwerp University Hospital, Edegem, Belgium.
authors.forename: Zwi N
authors.initials: ZN
authors.lastname: Berneman
mesh_terms: D019496:Cancer Vaccines; D002478:Cells, Cultured; D015925:Cryopreservation; D003713:Dendritic Cells; D018274:Electroporation; D006801:Humans; D012333:RNA, Messenger; D014611:Vaccination; D025721:WT1 Proteins / Q000235:genetics; D009396:Wilms Tumor / Q000628:therapy*
chemical_list: D019496:Cancer Vaccines; D012333:RNA, Messenger; D025721:WT1 Proteins; C583751:WT1 protein, human
keywords: Clinical grade; Dendritic cells; Electroporation; RNA; WT1; Wilms’ tumor 1