title: Endoplasmic reticulum stress and renal lesion in mice with combination of high-fat diet and streptozotocin-induced diabetes.
abstract: OBJECTIVE
  To investigate in the kidney the pathologic changes and expression of GRP78 and CHOP in the Kunming (KM) mice with combination of high-fat diet and streptozotocin-induced diabetes.
  
  
  METHODS
  Sixty two male KM mice were randomly divided into a normal control (NC) group (n=20) and a high-fat diet (HFD) group (n=42). After a four-week dietary manipulation, the KM mice in the HFD group were injected intraperitoneally with streptozotocin to induce diabetes. After diabetic models were successfully established, the kidneys were excised and conserved for further test.
  
  
  RESULTS
  No significant difference in the body weight was observed after the dietary manipulation (p=0.554). After the streptozotocin was injected, fasting blood glucose levels in the diabetes group (DM) were significantly higher than that in the NC group (p＜0.0001). Glomerular atrophy observed under light microscope in the DM group was more serious compared with the NC group. The expression of GRP78 and CHOP in the kidneys of the mice in the DM group were higher compared with the NC group.
  
  
  CONCLUSIONS
  Renal lesion occurs in the diabetic Kunming mice induced by combination of high-fat diet and low-dose streptozotocin, and endoplasmic reticulum stress and CHOP may contribute to the injury process.
authors.affiliation: Faculty of Nursing, Bengbu Medical College, Bengbu, Anhui, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xie
authors.affiliation: Faculty of Nursing, Bengbu Medical College, Bengbu, Anhui, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Huang
authors.affiliation: Faculty of Nursing, Bengbu Medical College, Bengbu, Anhui, China.
authors.forename: Yayun
authors.initials: Y
authors.lastname: Li
authors.affiliation: Faculty of Nursing, Bengbu Medical College, Bengbu, Anhui, China.
authors.forename: Han
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Faculty of Pharmacy, Bengbu Medical College, Bengbu, Anhui, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight / Q000502:physiology; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D003928:Diabetic Nephropathies / Q000378:metabolism* / Q000473:pathology*; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D000091342:Endoplasmic Reticulum Chaperone BiP; D059865:Endoplasmic Reticulum Stress / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000378:metabolism; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D011897:Random Allocation; D051742:Transcription Factor CHOP / Q000378:metabolism; D056811:Unfolded Protein Response / Q000502:physiology
chemical_list: D001786:Blood Glucose; D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; C000717248:Hspa5 protein, mouse; D051742:Transcription Factor CHOP