title: Convenient broad-host-range unstable vectors for studying stabilization cassettes in diverse bacteria.
abstract: BACKGROUND
  Low-copy-number vectors of potential wide application in biotechnology need to encode stabilization modules ensuring their stable inheritance. The efficiency of stabilization may vary depending on the plasmid host so a thorough analysis of stabilization functions is required before use.
  
  
  RESULTS
  To facilitate such analysis highly unstable, mobilizable, broad-host-range (BHR) vectors based on RK2 replicon were constructed. The vectors are suitable for testing of various stabilization functions, including plasmid and chromosomal partitioning cassettes encoding ParB homologues capable of spreading on DNA. The xylE or lacZ reporter systems facilitate easy monitoring of plasmid segregation.
  
  
  CONCLUSIONS
  The range of BHR vectors with different reporter cassettes and alternative mobilization systems expands their application in diverse bacterial species.
authors.affiliation: Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland.
authors.forename: Aneta A
authors.initials: AA
authors.lastname: Bartosik
authors.affiliation: Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Glabski
authors.affiliation: Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland.|Present address: Faculty of Chemistry, Institute of Biotechnology, Warsaw University of Technology, Warsaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Kulinska
authors.affiliation: Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Lewicka
authors.affiliation: Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland.|Present address: Faculty of Human Nutrition and Consumer Sciences, Laboratory of Food Chemistry, Warsaw University of Life Sciences, Warsaw, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Godziszewska
authors.affiliation: Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland.|, Present address: Pulawska 255A/4, 02-740, Warsaw, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Markowska
authors.affiliation: Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland. gjburdzy@ibb.waw.pl.
authors.forename: Grazyna
authors.initials: G
authors.lastname: Jagura-Burdzy
mesh_terms: D001419:Bacteria / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D005822:Genetic Vectors; D010957:Plasmids / Q000235:genetics*; D011815:R Factors; D012093:Replicon
chemical_list: D001426:Bacterial Proteins; C052883:chromosome partition proteins, bacterial
keywords: Broad-host-range; Cloning vector; RK2; Stability functions; lacZ; xylE reporter