title: In Vitro Cytotoxicity Evaluation of Three Root-End Filling Materials in Human Periodontal Ligament Fibroblasts.
abstract: The aim of this study was to evaluate in vitro the cytotoxicity on human periodontal ligament fibroblasts of three root-end filling materials: MTA Angelus®, EndoSequence Root Repair Material Putty® and Super EBA®. A primary culture of human periodontal ligament fibroblasts was previously obtained in order to evaluate the cytotoxicity of the three extracts from the root-end filling materials after 2 and 7 days of setting. Serial dilutions of these extracts (1:1, 1:2, 1:4 and 1:8) were evaluated at 1, 3 and 7 days using the methyl-thiazol-tetrazolium (MTT) colorimetric assay. Cell viability was evaluated as percentage of the negative control group, which represented 100% cell viability. Statistical analyses were done with t-test, ANOVA and Kruskal-Wallis test at a significance level of 5%. It was found that the main difference among root-end filling materials was in the higher dilutions (p<0.05), but there was a similar behavior in lower dilutions (p>0.05). Cell viability of MTA Angelus® was superior for 2-day setting (p<0.05), compared with the other two root-end fillings. There were no statistically significant differences between 7-day set MTA Angelus® and EndoSequence Root Repair Material Putty®. Super EBA® showed the lowest percentage of cell viability at higher dilutions (p<0.05). Therefore, MTA Angelus® and EndoSequence Root Repair Material Putty® were less cytotoxic in the highest dilution (1:1) compared with Super EBA®.
authors.affiliation: Department of Endodontics, Dental School, Universidad Peruana Cayetano Heredia, Lima, Peru.
authors.forename: Hernán
authors.initials: H
authors.lastname: Coaguila-Llerena
authors.affiliation: Laboratories for Research and Development, School of Sciences and Philosophy, Universidad Peruana Cayetano Heredia, Lima, Peru.
authors.forename: Abraham
authors.initials: A
authors.lastname: Vaisberg
authors.affiliation: Department of Endodontics, Dental School, Universidad Peruana Cayetano Heredia, Lima, Peru.
authors.forename: Zulema
authors.initials: Z
authors.lastname: Velásquez-Huamán
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D066298:In Vitro Techniques; D010513:Periodontal Ligament / Q000473:pathology*; D012387:Root Canal Filling Materials
chemical_list: D012387:Root Canal Filling Materials