title: Myosin II governs collective cell migration behaviour downstream of guidance receptor signalling.
abstract: Border cell migration during Drosophila oogenesis is a potent model to study collective cell migration, a process involved in development and metastasis. Border cell clusters adopt two main types of behaviour during migration: linear and rotational. However, the molecular mechanism controlling the switch from one to the other is unknown. Here, we demonstrate that non-muscle Myosin II (NMII, also known as Spaghetti squash) activity controls the linear-to-rotational switch. Furthermore, we show that the regulation of NMII takes place downstream of guidance receptor signalling and is critical to ensure efficient collective migration. This study thus provides new insight into the molecular mechanism coordinating the different cell behaviours in a migrating cluster.
authors.affiliation: LBCMCP, Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, 31062 Toulouse, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Combedazou
authors.affiliation: LBCMCP, Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, 31062 Toulouse, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Choesmel-Cadamuro
authors.affiliation: DamCB, Data Analysis and Modelling for Cell Biology, Marseille F-13005, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Gay
authors.affiliation: LBCMCP, Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, 31062 Toulouse, France.
authors.forename: Jiaying
authors.identifier: 0000-0002-4340-7746
authors.initials: J
authors.lastname: Liu
authors.affiliation: INSERM, UMR 1043, Centre de Physiopathologie de Toulouse Purpan, 31024 Toulouse, France.|Université Toulouse III Paul-Sabatier, 31062 Toulouse, France.|CNRS, UMR 5282, 31204 Toulouse, France.
authors.forename: Loïc
authors.initials: L
authors.lastname: Dupré
authors.affiliation: LBCMCP, Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, 31062 Toulouse, France.
authors.forename: Damien
authors.identifier: 0000-0002-9089-1497
authors.initials: D
authors.lastname: Ramel
authors.affiliation: LBCMCP, Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, 31062 Toulouse, France xiaobo.wang@univ-tlse3.fr.
authors.forename: Xiaobo
authors.identifier: 0000-0002-3311-1378
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D002465:Cell Movement; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology* / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism; D024462:Myosin Type II / Q000378:metabolism*; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism*; D012399:Rotation; D015398:Signal Transduction; D059008:Time-Lapse Imaging
chemical_list: D029721:Drosophila Proteins; C495593:Sqh protein, Drosophila; D066246:ErbB Receptors; C438368:Pvr protein, Drosophila; D020794:Receptor Protein-Tyrosine Kinases; D024462:Myosin Type II
keywords: Border cells; Collective cell migration; Guidance receptor; Myosin II