title: [THE COMPARATIVE ANALYSIS OF TECHNIQUES OF IDENTIFICATION OF CORYNEBACTERIUM NON DIPHTHERIAE].
abstract: The comparative analysis was carried out concerning effectiveness of three techniques of identification of Corynebacterium non diphtheriae: bacteriological, molecular genetic (sequenation on 16SpRNA) andmass-spectrometric (MALDI-ToFMS). The analysis covered 49 strains of Corynebacterium non diphtheriae (C.pseudodiphheriticum, C.amycolatum, C.propinquum, C.falsenii) and 2 strains of Corynebacterium diphtheriae isolated under various pathology form urogenital tract and upper respiratory ways. The corinbacteria were identified using bacteriologic technique, sequenation on 16SpRNA and mass-spectrometric technique (MALDIToF MS). The full concordance of results of species' identification was marked in 26 (51%) of strains of Corynebacterium non diphtheriae at using three analysis techniques; in 43 (84.3%) strains--at comparison of bacteriologic technique with sequenation on 16S pRNA and in 29 (57%)--at mass-spectrometric analysis and sequenation on 16S pRNA. The bacteriologic technique is effective for identification of Corynebacterium diphtheriae. The precise establishment of species belonging of corynebacteria with variable biochemical characteristics the molecular genetic technique of analysis is to be applied. The mass-spectrometric technique (MALDI-ToF MS) requires further renewal of data bases for identifying larger spectrum of representatives of genus Corynebacterium.
authors.forename: G G
authors.initials: GG
authors.lastname: Kharseeva
authors.forename: N A
authors.initials: NA
authors.lastname: Voronina
authors.forename: A Yu
authors.initials: AY
authors.lastname: Mironov
authors.forename: E L
authors.initials: EL
authors.lastname: Alutina
mesh_terms: D015373:Bacterial Typing Techniques / Q000295:instrumentation / Q000379:methods*; D003352:Corynebacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D003354:Corynebacterium Infections / Q000382:microbiology; D003353:Corynebacterium diphtheriae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D006801:Humans; D008853:Microscopy; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012141:Respiratory Tract Infections / Q000382:microbiology; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014552:Urinary Tract Infections / Q000382:microbiology
chemical_list: D012336:RNA, Ribosomal, 16S