title: Real Time Gabor-Domain Optical Coherence Microscopy for 3D Imaging.
abstract: Fast, robust, nondestructive 3D imaging is needed for the characterization of microscopic tissue structures across various clinical applications. A custom microelectromechanical system (MEMS)-based 2D scanner was developed to achieve, together with a multi-level GPU architecture, 55 kHz fast-axis A-scan acquisition in a Gabor-domain optical coherence microscopy (GD-OCM) custom instrument. GD-OCM yields high-definition micrometer-class volumetric images. A dynamic depth of focusing capability through a bio-inspired liquid lens-based microscope design, as in whales' eyes, was developed to enable the high definition instrument throughout a large field of view of 1 mm3 volume of imaging. Developing this technology is prime to enable integration within the workflow of clinical environments. Imaging at an invariant resolution of 2 μm has been achieved throughout a volume of 1 × 1 × 0.6 mm3, acquired in less than 2 minutes. Volumetric scans of human skin in vivo and an excised human cornea are presented.
authors.affiliation: University of Rochester, Rochester, NY, USA 14627.
authors.forename: Jannick P
authors.initials: JP
authors.lastname: Rolland
authors.affiliation: LighTopTech Corp., West Henrietta, NY, USA 14586.
authors.forename: Cristina
authors.initials: C
authors.lastname: Canavesi
authors.affiliation: University of Rochester, Rochester, NY, USA 14627.
authors.forename: Patrice
authors.initials: P
authors.lastname: Tankam
authors.affiliation: University of Rochester, Rochester, NY, USA 14627.
authors.forename: Andrea
authors.initials: A
authors.lastname: Cogliati
authors.affiliation: University of Rochester, Rochester, NY, USA 14627.
authors.forename: Mara
authors.initials: M
authors.lastname: Lanis
authors.affiliation: University of California, Los Angeles, CA, USA 90095.
authors.forename: Anand P
authors.initials: AP
authors.lastname: Santhanam
mesh_terms: D003199:Computer Systems; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000295:instrumentation* / Q000379:methods; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation* / Q000379:methods; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*; D041623:Tomography, Optical Coherence / Q000295:instrumentation* / Q000379:methods