title: [Adiponectin exerts antiproliferative effect on high glucose-induced BeWo cell proliferation in vitro].
abstract: OBJECTIVE
  To determine the effects of adiponectin on high glucose induced BeWo cell proliferation in vitro.
  
  
  METHODS
  BeWo cells were seeded in 96-well plates at the appropriate density. After treatments with high glucose (25 mmol/L), western blot analysis of cyclin D1 and a colorimetric assay (cell counting kit-8, CCK-8) were used to analyse BeWo cells' proliferation, and western blot was used to detect the expression of adiponectin. Moreover, we added adiponectin (20 μg/ml) in the culture medium and three methods were utilized for cell proliferation analysis: CCK-8, cell cycle analysis (by flow cytometry) and proliferating cell nuclear antigen (PCNA) immunocytochemical staining.
  
  
  RESULTS
  Compared to BeWo cells cultured by normal glucose and high mannitol, the proliferation of BeWo cells treated by high glucose increased (P<0.05). Compared with BeWo cells cultured by high mannitol, the expression of adiponectin in BeWo cells treated by high glucose decreased. After added adiponectin in the culture medium, the proliferation of BeWo cells treated by adiponectin+high glucose decreased than that of cells treated by high glucose (0.770±0.050 versus 0.990±0.070, P<0.05); the proportion of G2+S phases of BeWo cells treated by adiponectin+high glucose decreased than that of cells treated by high glucose [(40.7±2.1)% versus (44.9± 3.9)%, P<0.05]; the rate of PCNA positive cell in BeWo cells treated by adiponectin+high glucose decreased than that of cells treated by high glucose [(28±5)% versus (44±5)%, P<0.05].
  
  
  CONCLUSIONS
  Adiponectin could inhibit proliferation of high glucose induced BeWo cells in vitro.
authors.affiliation: Department of Obstetrics and Gynecology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: H T
authors.initials: HT
authors.lastname: Chen
authors.forename: D Y
authors.initials: DY
authors.lastname: Wang
authors.forename: H Z
authors.initials: HZ
authors.lastname: Geng
authors.forename: H Q
authors.initials: HQ
authors.lastname: Chen
authors.forename: Y X
authors.initials: YX
authors.lastname: Wu
authors.forename: S Q
authors.initials: SQ
authors.lastname: Deng
authors.forename: Z L
authors.initials: ZL
authors.lastname: Wang
mesh_terms: D052242:Adiponectin / Q000378:metabolism*; D015153:Blotting, Western; D002453:Cell Cycle; D049109:Cell Proliferation / Q000502:physiology*; D005434:Flow Cytometry; D005947:Glucose; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin; D018809:Proliferating Cell Nuclear Antigen; D005947:Glucose