title: [Changes of CD3γ, CD3δ and CD3epsilon chains mRNAs in lead-poisoned patients after chelate treatment].
abstract: OBJECTIVE
  To investigate the expressions of T cell receptor (TCR) signaling CD3γ, CD3δ and CD3epsilon chains in T cells from peripheral blood mononuclear cells (PBMCs) in lead-poisoned workers after chelate treatment.
  
  
  METHODS
  The expression levels of CD3γ, CD3δ and CD3epsilon mRNAs in PBMCs from 16 lead-poisoned workers before and after chelate treatment and 9 healthy individuals were detected by real-time quantitative PCR. The β2-microglobulin gene (β2M) was used as an endogenous reference. Relative mRNA levels were calculated by the 2(-δCt)×100%. Spearman correlation analysis was used to estimate the correlations of different gene expression levels between healthy individuals and the patients as well as before and after the treatment.
  
  
  RESULTS
  The CD3γ mRNA level in the lead-poisoned workers before and after chelate treatment has no significant difference as compared with that of the healthy individuals. The expression levels of CD3δ mRNA in the lead-poisoning workers before and after chelate treatment were remarkably lower than that of the healthy individuals. After chelate treatment, the CD3δ mRNA level in the lead-poisoned workers was lower than that before treatment. The CD3epsilon mRNA level in the lead-poisoned workers before treatment was obviously higher than that of the healthy individuals, while the expression level of CD3epsilon mRNA decreased after chelate treatment. There were no evident correlations between CD3γ and CD3δ, CD3γ and CD3epsilon, CD3δ and CD3epsilon mRNAs, while CD3γ and CD3δ, CD3γ and CD3epsilon, CD3δ and CD3epsilon mRNAs had markedly positive correlations in the lead-poisoned workers before chelate treatment, and only CD3δ and CD3epsilon had a significantly positive correlation after the treatment.
  
  
  CONCLUSIONS
  The expression levels of CD3γ, CD3δ and CD3epsilon mRNAs in the lead-poisoned workers showed varied alterations, which indicated that lead poisoning had an impact on human immunity system and affected different genes in different ways. After chelate treatment, CD3δ mRNA level was still different from that of healthy individuals, which indicated that the function of the immunity system was still abnormal.
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou 510632, China.
authors.forename: Lixing
authors.initials: L
authors.lastname: Guo
authors.affiliation: Ministry of Education Key Laboratory for Regenerative Medicine, Jinan University, Guangzhou 510632, China.
authors.forename: Yankai
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou 510632, China.
authors.forename: Sichu
authors.initials: S
authors.lastname: Liu
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou 510632; Ministry of Education Key Laboratory for Regenerative Medicine, Jinan University, Guangzhou 510632, China. *Corresponding author, E-mail: jnlibo517@163.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.affiliation: Guangzhou Twelfth People's Hospital, Guangzhou 510620, China.
authors.forename: Zhiqian
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Guangzhou Twelfth People's Hospital, Guangzhou 510620, China.
authors.forename: Yili
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou 510632; Ministry of Education Key Laboratory for Regenerative Medicine, Jinan University, Guangzhou 510632, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Liu
authors.forename: Yangqiu
authors.initials: Y
authors.lastname: Li
mesh_terms: D000328:Adult; D017252:CD3 Complex / Q000235:genetics* / Q000276:immunology; D016022:Case-Control Studies; D002614:Chelating Agents / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D007855:Lead Poisoning / Q000188:drug therapy* / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D055815:Young Adult
chemical_list: D017252:CD3 Complex; C452842:CD3 antigen, gamma chain; C115335:CD3E protein, human; C472562:CD3delta antigen; D002614:Chelating Agents