title: DDX21 translocates from nucleus to cytoplasm and stimulates the innate immune response due to dengue virus infection.
abstract: Successful DENV infection relies on its ability to evade the host innate immune system. By using iTRAQ labeling followed by LC-MS/MS analysis, DDX21 was identified as a new host RNA helicase involved in the DENV life cycle. In DENV infected cells, DDX21 translocates from nucleus to cytoplasm to active the innate immune response and thus inhibits DENV replication in the early stages of infection. DDX21 is then degraded by the viral NS2B-NS3 protease complex and the innate immunity is thus subverted to facilitate DENV replication. The results reveal a new mechanism in which DENV subverts the host innate immune system to facilitate its replication in host cells.
authors.affiliation: The Department of Microbiology, Faculty of Preclinical Medicine, The Fourth Military Medical University, Xian, Shaanxi, 710032, China.
authors.forename: Yangchao
authors.initials: Y
authors.lastname: Dong
authors.affiliation: The Department of Microbiology, Faculty of Preclinical Medicine, The Fourth Military Medical University, Xian, Shaanxi, 710032, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ye
authors.affiliation: The Department of Microbiology, Faculty of Preclinical Medicine, The Fourth Military Medical University, Xian, Shaanxi, 710032, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: The Department of Microbiology, Faculty of Preclinical Medicine, The Fourth Military Medical University, Xian, Shaanxi, 710032, China.
authors.forename: Peijun
authors.initials: P
authors.lastname: Han
authors.affiliation: The Department of Microbiology, Faculty of Preclinical Medicine, The Fourth Military Medical University, Xian, Shaanxi, 710032, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The Department of Microbiology, Faculty of Preclinical Medicine, The Fourth Military Medical University, Xian, Shaanxi, 710032, China.
authors.forename: Chuantao
authors.initials: C
authors.lastname: Ye
authors.affiliation: The Department of Microbiology, Faculty of Preclinical Medicine, The Fourth Military Medical University, Xian, Shaanxi, 710032, China.
authors.forename: Daihui
authors.initials: D
authors.lastname: Weng
authors.affiliation: The Department of Microbiology, Faculty of Preclinical Medicine, The Fourth Military Medical University, Xian, Shaanxi, 710032, China.
authors.forename: Fanglin
authors.initials: F
authors.lastname: Zhang
authors.affiliation: The Department of Microbiology, Faculty of Preclinical Medicine, The Fourth Military Medical University, Xian, Shaanxi, 710032, China. Electronic address: zhikaixu523@163.com.
authors.forename: Zhikai
authors.initials: Z
authors.lastname: Xu
authors.affiliation: The Department of Microbiology, Faculty of Preclinical Medicine, The Fourth Military Medical University, Xian, Shaanxi, 710032, China. Electronic address: yflei@fmmu.edu.cn.
authors.forename: Yingfeng
authors.initials: Y
authors.lastname: Lei
mesh_terms: D002460:Cell Line; D053487:DEAD-box RNA Helicases / Q000276:immunology* / Q000378:metabolism; D003715:Dengue / Q000276:immunology* / Q000378:metabolism; D003716:Dengue Virus / Q000276:immunology* / Q000502:physiology; D006801:Humans; D007113:Immunity, Innate; D021381:Protein Transport; D059748:Proteolysis; D014779:Virus Replication
chemical_list: C501726:DDX21 protein, human; D053487:DEAD-box RNA Helicases
keywords: DDX21; DENV; Innate immune response; NS2B/3 protease; iTRAQ