title: Biofluid infrared spectro-diagnostics: pre-analytical considerations for clinical applications.
abstract: Several proof-of-concept studies on the vibrational spectroscopy of biofluids have demonstrated that the methodology has promising potential as a clinical diagnostic tool. However, these studies also show that there is a lack of a standardised protocol in sample handling and preparation prior to spectroscopic analysis. One of the most important sources of analytical errors is the pre-analytical phase. For the technique to be translated into clinics, it is clear that a very strict protocol needs to be established for such biological samples. This study focuses on some of the aspects of the pre-analytical phase in the development of the high-throughput Fourier Transform Infrared (FTIR) spectroscopy of some of the most common biofluids such as serum, plasma and bile. Pre-analytical considerations that can impact either the samples (solvents, anti-coagulants, freeze-thaw cycles…) and/or spectroscopic analysis (sample preparation such as drying, deposit methods, volumes, substrates, operators dependence…) and consequently the quality and the reproducibility of spectral data will be discussed in this report.
authors.affiliation: Université de Reims Champagne-Ardenne, MéDIAN-Biophotonique et Technologies pour la Santé, UFR de Pharmacie, 51 rue Cognacq-Jay, 51095 Reims Cedex, France. ganesh.sockalingum@univ-reims.fr and CNRS UMR 7369, Matrice extracellulaire et Dynamique Cellulaire, MEDyC, 51096 Reims Cedex, France and WESTChem, Department of Pure and Applied Chemistry, Technology and Innovation Centre, University of Strathclyde, Glasgow, G1 1RD, UK.
authors.forename: L
authors.initials: L
authors.lastname: Lovergne
authors.affiliation: SATT NORD (Société d'Accélération du Transfert de Technologie), 4 bd de la Paix, 51100 Reims, France.
authors.forename: P
authors.initials: P
authors.lastname: Bouzy
authors.affiliation: Université de Reims Champagne-Ardenne, MéDIAN-Biophotonique et Technologies pour la Santé, UFR de Pharmacie, 51 rue Cognacq-Jay, 51095 Reims Cedex, France. ganesh.sockalingum@univ-reims.fr and CNRS UMR 7369, Matrice extracellulaire et Dynamique Cellulaire, MEDyC, 51096 Reims Cedex, France and Plateforme en imagerie cellulaire et tissulaire (PICT), Université de Reims Champagne-Ardenne, 51 rue Cognacq-Jay, 51096 Reims Cedex, France.
authors.forename: V
authors.initials: V
authors.lastname: Untereiner
authors.affiliation: Université de Reims Champagne-Ardenne, MéDIAN-Biophotonique et Technologies pour la Santé, UFR de Pharmacie, 51 rue Cognacq-Jay, 51095 Reims Cedex, France. ganesh.sockalingum@univ-reims.fr and CNRS UMR 7369, Matrice extracellulaire et Dynamique Cellulaire, MEDyC, 51096 Reims Cedex, France and Laboratoire de Biologie et Recherche Pédiatriques, CHU de Reims, 51092 Reims Cedex, France.
authors.forename: R
authors.initials: R
authors.lastname: Garnotel
authors.affiliation: WESTChem, Department of Pure and Applied Chemistry, Technology and Innovation Centre, University of Strathclyde, Glasgow, G1 1RD, UK.
authors.forename: M J
authors.initials: MJ
authors.lastname: Baker
authors.affiliation: Université de Reims Champagne-Ardenne, MéDIAN-Biophotonique et Technologies pour la Santé, UFR de Pharmacie, 51 rue Cognacq-Jay, 51095 Reims Cedex, France. ganesh.sockalingum@univ-reims.fr and CNRS UMR 7369, Matrice extracellulaire et Dynamique Cellulaire, MEDyC, 51096 Reims Cedex, France and Service d'Hépato-Gastroentérologie, CHU de Reims, Hôpital Robert Debré, 51092 Reims Cedex, France.
authors.forename: G
authors.initials: G
authors.lastname: Thiéfin
authors.affiliation: Université de Reims Champagne-Ardenne, MéDIAN-Biophotonique et Technologies pour la Santé, UFR de Pharmacie, 51 rue Cognacq-Jay, 51095 Reims Cedex, France. ganesh.sockalingum@univ-reims.fr and CNRS UMR 7369, Matrice extracellulaire et Dynamique Cellulaire, MEDyC, 51096 Reims Cedex, France.
authors.forename: G D
authors.initials: GD
authors.lastname: Sockalingum
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods* / Q000592:standards*; D000925:Anticoagulants / Q000737:chemistry; D001646:Bile / Q000737:chemistry; D001826:Body Fluids / Q000737:chemistry* / Q000000981:diagnostic imaging*; D005615:Freezing; D006801:Humans; D010949:Plasma / Q000737:chemistry; D015203:Reproducibility of Results; D044967:Serum / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods* / Q000592:standards*; D014732:Vibration
chemical_list: D000925:Anticoagulants; D012997:Solvents