title: QTL mapping of soybean oil content for marker-assisted selection in plant breeding program.
abstract: The present study was undertaken to detect and map the quantitative trait loci (QTL) related to soybean oil content. We used 244 progenies derived from a bi-parental cross of the Lineage 69 (from Universidade Estadual Paulista "Júlio de Mesquita Filho"/Faculdade de Ciências Agrárias e Veterinárias - Breeding Program) and Tucunaré cultivar. A total of 358 simple sequence repeat (SSR; microsatellite) markers were used to investigate the polymorphism between the parental lines, and for the polymorphic lines all the F2 individuals were tested. Evaluation of the oil content and phenotype was performed with the aid of a Tango equipment by near infra-red reflectance spectroscopy, using single F2 seeds and F2:3 progenies, in triplicate. The data were analyzed by QTL Cartographer program for 56 SSR polymorphic markers. Two oil-content related QTLs were detected on K and H linkage groups. The total phenotypic variation explained by QTLs ranged from 7.8 to 46.75% for oil content. New QTLs were identified for the oil content in addition to those previously identified in other studies. The results reported in this study show that regions different from those already known could be involved in the genetic control of soybean oil content.
authors.affiliation: Laboratório de Biotecnologia Aplicada ao Melhoramento de Plantas, Faculdade de Ciências Agrárias e Veterinárias.
authors.forename: D C
authors.initials: DC
authors.lastname: Leite
authors.affiliation: Laboratório de Diversidade Genética e Melhoramento de Plantas, Universidade de São Paulo, Escola Superior de Agricultura "Luiz de Queiroz", Piracicaba, SP, Brasil.
authors.forename: J B
authors.initials: JB
authors.lastname: Pinheiro
authors.affiliation: Laboratório de Diversidade Genética e Melhoramento de Plantas, Universidade de São Paulo, Escola Superior de Agricultura "Luiz de Queiroz", Piracicaba, SP, Brasil.
authors.forename: J B
authors.initials: JB
authors.lastname: Campos
authors.affiliation: Laboratório de Biotecnologia Aplicada ao Melhoramento de Plantas, Faculdade de Ciências Agrárias e Veterinárias.
authors.forename: A O
authors.initials: AO
authors.lastname: Di Mauro
authors.affiliation: Laboratório de Biotecnologia Aplicada ao Melhoramento de Plantas, Faculdade de Ciências Agrárias e Veterinárias.
authors.forename: S H
authors.initials: SH
authors.lastname: Unêda-Trevisoli
mesh_terms: D008040:Genetic Linkage; D005819:Genetic Markers; D018895:Microsatellite Repeats; D000069600:Plant Breeding; D010938:Plant Oils / Q000378:metabolism*; D040641:Quantitative Trait Loci; D012639:Seeds / Q000235:genetics / Q000378:metabolism; D012641:Selection, Genetic; D013025:Glycine max / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D010938:Plant Oils