title: SCF(SAP) controls organ size by targeting PPD proteins for degradation in Arabidopsis thaliana.
abstract: Control of organ size by cell proliferation and growth is a fundamental process, but the mechanisms that determine the final size of organs are largely elusive in plants. We have previously revealed that the ubiquitin receptor DA1 regulates organ size by repressing cell proliferation in Arabidopsis. Here we report that a mutant allele of STERILE APETALA (SAP) suppresses the da1-1 mutant phenotype. We show that SAP is an F-box protein that forms part of a SKP1/Cullin/F-box E3 ubiquitin ligase complex and controls organ size by promoting the proliferation of meristemoid cells. Genetic analyses suggest that SAP may act in the same pathway with PEAPOD1 and PEAPOD2, which are negative regulators of meristemoid proliferation, to control organ size, but does so independently of DA1. Further results reveal that SAP physically associates with PEAPOD1 and PEAPOD2, and targets them for degradation. These findings define a molecular mechanism by which SAP and PEAPOD control organ size.
authors.affiliation: State Key Laboratory of Plant Cell and Chromosome Engineering, CAS Center for Excellence in Molecular Plant Sciences, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Lincui East Road, Chaoyang District, Beijing 100101, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Zhibiao
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Plant Cell and Chromosome Engineering, CAS Center for Excellence in Molecular Plant Sciences, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Lincui East Road, Chaoyang District, Beijing 100101, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: State Key Laboratory of Plant Cell and Chromosome Engineering, CAS Center for Excellence in Molecular Plant Sciences, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Lincui East Road, Chaoyang District, Beijing 100101, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Department of Plant Systems Biology, VIB, Technologiepark 927, Ghent 9052, Belgium.|Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, Ghent 9052, Belgium.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Gonzalez
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xiahe
authors.initials: X
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Plant Systems Biology, VIB, Technologiepark 927, Ghent 9052, Belgium.|Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, Ghent 9052, Belgium.
authors.forename: Dirk
authors.initials: D
authors.lastname: Inzé
authors.affiliation: State Key Laboratory of Plant Cell and Chromosome Engineering, CAS Center for Excellence in Molecular Plant Sciences, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Lincui East Road, Chaoyang District, Beijing 100101, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Yunhai
authors.identifier: http://orcid.org/0000-0002-0025-4444
authors.initials: Y
authors.lastname: Li
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D049109:Cell Proliferation; D044842:Cullin Proteins / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D044783:F-Box Proteins / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D060588:LIM Domain Proteins / Q000235:genetics / Q000378:metabolism; D018519:Meristem / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D009929:Organ Size; D063246:Organogenesis, Plant / Q000235:genetics; D010641:Phenotype; D059828:Plant Cells / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D059748:Proteolysis; D044843:SKP Cullin F-Box Protein Ligases / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D044842:Cullin Proteins; C529975:DA1 protein, Arabidopsis; D004268:DNA-Binding Proteins; D044783:F-Box Proteins; D060588:LIM Domain Proteins; C513798:PEAPOD protein, Arabidopsis; C119016:SAP protein, Arabidopsis; D029701:Saccharomyces cerevisiae Proteins; C000593019:TIFY4B protein, Arabidopsis; D014157:Transcription Factors; D044843:SKP Cullin F-Box Protein Ligases; C120507:SKP1 protein, S cerevisiae; D044767:Ubiquitin-Protein Ligases; D046988:Proteasome Endopeptidase Complex