title: Expression of cartilage antitumor component RanBP9 in osteosarcoma.
abstract: This study was carried out to investigate the expression changes of RanBP9 in tissue specimens and osteosarcoma cell strains and preliminarily explore its mechanism in osteosarcoma, so as to provide a theoretical foundation for follow-up experiments. The expression of RanBP9 in human osteosarcoma tissue specimens was detected by immunohistochemistry and the expression of RanBP9 messenger ribose nucleic acid (mRNA) in osteosarcoma cell strains was detected in real-time with polymerase chain reaction (PCR), and finally the expression of RanBP9 protein in osteosarcoma cell strains was detected by immunofluorescent staining and Western blot. Results demonstrated that RanBP9 was widely expressed in tissues, but also highly expressed in cells; moreover, the expression of RanBP9 was mainly concentrated in cytoplasm and nucleus, and partial expression was found in cell membrane. Thus, it can be concluded that RanBP9 is positively expressed in bone tumor tissues and cell strains.
authors.affiliation: Department of Orthopaedics, the Peoples Hospital of Liaocheng City, Shandong, China.
authors.forename: L L
authors.initials: LL
authors.lastname: Zhu
authors.affiliation: Department of Orthopaedics, the Peoples Hospital of Liaocheng City, Shandong, China.
authors.forename: C H
authors.initials: CH
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, the Peoples Hospital of Liaocheng City, Shandong, China.
authors.forename: H P
authors.initials: HP
authors.lastname: Yang
authors.affiliation: Medical Basic Teaching and Research Office, School of Nursing, Binzhou Polytechnic, Shandong, China.
authors.forename: W H
authors.initials: WH
authors.lastname: Shu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D002356:Cartilage / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D003598:Cytoskeletal Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D010010:Osteoclasts / Q000378:metabolism; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; C116005:Ran binding protein 9