title: Anti-Interleukin-1 Beta/Tumor Necrosis Factor-Alpha IgY Antibodies Reduce Pathological Allergic Responses in Guinea Pigs with Allergic Rhinitis.
abstract: This study aims to determine whether the combined blockade of IL-1β and TNF-α can alleviate the pathological allergic inflammatory reaction in the nasal mucosa and lung tissues in allergic rhinitis (AR) guinea pigs. Healthy guinea pigs treated with saline were used as the healthy controls. The AR guinea pigs were randomly divided into (1) the AR model group treated with intranasal saline; (2) the 0.1% nonspecific IgY treatment group; (3) the 0.1% anti-TNF-α IgY treatment group; (4) the 0.1% anti-IL-1β IgY treatment group; (5) the 0.1% combined anti-IL-1β and TNF-α IgY treatment group; and (6) the fluticasone propionate treatment group. The inflammatory cells were evaluated using Wright's staining. Histopathology was examined using hematoxylin-eosin staining. The results showed that the number of eosinophils was significantly decreased in the peripheral blood, nasal lavage fluid, and bronchoalveolar lavage fluid (P < 0.05), and eosinophil, neutrophil, and lymphocyte infiltration and edema were significantly reduced or absent in the nasal mucosa and lung tissues (P < 0.05) in the combined 0.1% anti-IL-1β- and TNF-α IgY-treated guinea pigs. The data suggest that topical blockade of IL-1β and TNF-α could reduce pathological allergic inflammation in the nasal mucosa and lung tissues in AR guinea pigs.
authors.affiliation: Department of Radiation Oncology, Shanghai Proton and Heavy Ion Center, Shanghai 201315, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Wei-Xu
authors.affiliation: Institute of Clinical Medical Sciences, Jiangxi Province People's Hospital, Nanchang, Jiangxi 330006, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Wen-Yun
authors.affiliation: Institute of Clinical Medical Sciences, Jiangxi Province People's Hospital, Nanchang, Jiangxi 330006, China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Xi-Ling
authors.affiliation: Department of Hematology, Jiangxi Academy of Medical Science, Nanchang, Jiangxi 330006, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Institute of Clinical Medical Sciences, Jiangxi Province People's Hospital, Nanchang, Jiangxi 330006, China.
authors.forename: Wu
authors.initials: W
authors.lastname: Li-Hua
authors.affiliation: Institute of Clinical Medical Sciences, Jiangxi Province People's Hospital, Nanchang, Jiangxi 330006, China.
authors.forename: Wu
authors.initials: W
authors.lastname: Xiao-Mu
authors.affiliation: Institute of Clinical Medical Sciences, Jiangxi Province People's Hospital, Nanchang, Jiangxi 330006, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hui-Ping
authors.affiliation: Institute of Clinical Medical Sciences, Jiangxi Province People's Hospital, Nanchang, Jiangxi 330006, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Wen-Ding
authors.affiliation: Institute of Clinical Medical Sciences, Jiangxi Province People's Hospital, Nanchang, Jiangxi 330006, China.
authors.forename: He
authors.initials: H
authors.lastname: Dan
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha, Hunan 410082, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Qin
authors.affiliation: Institute of Clinical Medical Sciences, Jiangxi Province People's Hospital, Nanchang, Jiangxi 330006, China.
authors.forename: Hu
authors.identifier: 0000-0003-1525-353X
authors.initials: H
authors.lastname: Guo-Zhu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006168:Guinea Pigs; D007136:Immunoglobulins / Q000627:therapeutic use*; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors / Q000276:immunology*; D008168:Lung / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008297:Male; D009297:Nasal Mucosa / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D065631:Rhinitis, Allergic / Q000188:drug therapy* / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000276:immunology*
chemical_list: C016512:IgY; D007136:Immunoglobulins; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha