title: Posttranscriptional Regulation of PTEN by Competing Endogenous RNAs.
abstract: PTEN expression can be dysregulated in cancers via multiple mechanisms including genomic loss, epigenetic silencing, transcriptional repression, and posttranscriptional regulation by microRNAs. MicroRNAs are short, noncoding RNAs that regulate gene expression by binding to recognition sites on target transcripts. Recent studies have demonstrated that the competition for shared microRNAs between both protein-coding and noncoding transcripts represents an additional facet of gene regulation. Here, we describe in detail an integrated computational and experimental approach to identify and validate these competing endogenous RNA (ceRNA) interactions.
authors.affiliation: Cancer Science Institute of Singapore, Centre for Translational Medicine, National University of Singapore, 14 Medical Drive, #12-01, Singapore, 117599, Singapore. yvonnetay@nus.edu.sg.|Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117597, Singapore. yvonnetay@nus.edu.sg.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Tay
authors.affiliation: Cancer Research Institute, Beth Israel Deaconess Cancer Center, Department of Medicine and Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, 3 Blackfan Circle, Boston, MA, 02215, USA. ppandolf@bidmc.harvard.edu.
authors.forename: Pier Paolo
authors.initials: PP
authors.lastname: Pandolfi
mesh_terms: D000818:Animals; D019295:Computational Biology / Q000379:methods*; D005786:Gene Expression Regulation; D045325:HCT116 Cells; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics; D051059:PTEN Phosphohydrolase / Q000235:genetics*; D012313:RNA / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D012313:RNA; D051059:PTEN Phosphohydrolase
keywords: Competing endogenous RNA; MicroRNA; PTEN; ceRNA; miRNA