title: Matrix metalloproteinase 9 -1562C/T polymorphism increased protein levels in patients with colorectal cancer in a sample from southeastern Brazil.
abstract: We examined whether the allelic and/or genotypic profile of locus -1562C/T of the matrix metalloproteinase (MMP-9) gene influences the protein expression levels of MMP-9 in patients with colorectal cancer (CRC) compared with controls. A total of 104 patients with CRC and 84 controls were evaluated. Peripheral blood was collected from both groups and DNA extraction was performed for -1562C/T genotyping; the plasma was used for MMP-9 quantification. The CT genotype was associated with increased MMP-9 expression (P = 0.0211). High levels of protein, independently of polymorphisms, were observed in the patient group (P < 0.0001) compared to controls. Mucinous tumors with signet ring cells were more frequent in females (P = 0.0177). Overall, patients older than 50 years showed a significant risk of developing CRC (P = 0.0001). MMP-9 plasma expression was increased in patients with CRC compared to controls, particularly in those with the heterozygous -1562CT genotype.
authors.affiliation: Instituto de Ensino e Pesquisa da Santa Casa de Belo Horizonte, Belo Horizonte, MG, Brasil.
authors.forename: C R
authors.initials: CR
authors.lastname: Hoelzle
authors.affiliation: Instituto de Ensino e Pesquisa da Santa Casa de Belo Horizonte, Belo Horizonte, MG, Brasil.
authors.forename: K C S F
authors.initials: KC
authors.lastname: Magalhães
authors.affiliation: Instituto de Ensino e Pesquisa da Santa Casa de Belo Horizonte, Belo Horizonte, MG, Brasil.
authors.forename: S S
authors.initials: SS
authors.lastname: Carvalho
authors.affiliation: Instituto de Ensino e Pesquisa da Santa Casa de Belo Horizonte, Belo Horizonte, MG, Brasil.|Grupo de Coloproctologia do Hospital da Santa Casa de Belo Horizonte, Belo Horizonte, MG, Brasil.
authors.forename: G A
authors.initials: GA
authors.lastname: Santos
authors.affiliation: Instituto de Ensino e Pesquisa da Santa Casa de Belo Horizonte, Belo Horizonte, MG, Brasil.
authors.forename: I M
authors.initials: IM
authors.lastname: Maia
authors.affiliation: Instituto de Ensino e Pesquisa da Santa Casa de Belo Horizonte, Belo Horizonte, MG, Brasil.
authors.forename: M C P S
authors.initials: MC
authors.lastname: Sousa
authors.affiliation: Laboratório José de Souza Andrade Filho, Belo Horizonte, MG, Brasil.
authors.forename: J S
authors.initials: JS
authors.lastname: Andrade-Filho
authors.affiliation: Instituto de Ensino e Pesquisa da Santa Casa de Belo Horizonte, Belo Horizonte, MG, Brasil.|Grupo de Coloproctologia do Hospital da Santa Casa de Belo Horizonte, Belo Horizonte, MG, Brasil.
authors.forename: G M G
authors.initials: GM
authors.lastname: Cruz
authors.affiliation: Instituto de Ensino e Pesquisa da Santa Casa de Belo Horizonte, Belo Horizonte, MG, Brasil.
authors.forename: R T
authors.initials: RT
authors.lastname: Simões
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D001938:Brazil; D015179:Colorectal Neoplasms / Q000097:blood* / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000097:blood* / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D020780:Matrix Metalloproteinase 9