title: Rapid assignment of malting barley varieties by matrix-assisted laser desorption-ionisation - Time-of-flight mass spectrometry.
abstract: A method for discriminating malting barley varieties based on direct matrix-assisted laser desorption-ionisation - time-of-flight mass spectrometry (MALDI-TOF MS) fingerprinting of proteins was developed. Signals corresponding to hordeins were obtained by simple mixing of powdered barley grain with a MALDI matrix solution containing 12.5mgmL(-1) of ferulic acid in an acetonitrile:water:formic acid 50:33:17 v/v/v mixture. Compared to previous attempts at MALDI-TOF mass spectrometric analysis of barley proteins, the extraction and fractionation steps were practically omitted, resulting in a significant reduction in analytical time and costs. The discriminatory power was examined on twenty malting barley varieties and the practicability of the method was tested on sixty barley samples acquired from Pilsner Urquell Brewery. The method is proposed as a rapid tool for variety assignment and purity determination of malting barley that may replace gel electrophoresis currently used for this purpose.
authors.affiliation: Research Group Proteomics, CEITEC - Central European Institute of Technology, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic; National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic. Electronic address: sedo@sci.muni.cz.
authors.forename: Ondrej
authors.initials: O
authors.lastname: Šedo
authors.affiliation: Pilsner Urquell Brewery, U prazdroje 7, 30497 Plzeň, Czech Republic.
authors.forename: Michal
authors.initials: M
authors.lastname: Kořán
authors.affiliation: Research Group Proteomics, CEITEC - Central European Institute of Technology, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic.
authors.forename: Michaela
authors.initials: M
authors.lastname: Jakešová
authors.affiliation: Research Institute of Brewing and Malting, Malting Institute Brno, Mostecká 7, 61400 Brno, Czech Republic.
authors.forename: Renata
authors.initials: R
authors.lastname: Mikulíková
authors.affiliation: Bruker Daltonics, s.r.o., Pražákova 60, 61900 Brno, Czech Republic.
authors.forename: Michal
authors.initials: M
authors.lastname: Boháč
authors.affiliation: Research Group Proteomics, CEITEC - Central European Institute of Technology, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic; National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic. Electronic address: zdrahal@sci.muni.cz.
authors.forename: Zbyněk
authors.initials: Z
authors.lastname: Zdráhal
mesh_terms: D001515:Beer; D005525:Food-Processing Industry / Q000379:methods*; D005983:Glutens / Q000032:analysis*; D001467:Hordeum / Q000737:chemistry / Q000145:classification* / Q000378:metabolism; D036226:Seedlings / Q000737:chemistry / Q000145:classification* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D005983:Glutens
keywords: Barley; MALDI-TOF MS; Malting; Proteins