title: Magnesium Supplementation Diminishes Peripheral Blood Lymphocyte DNA Oxidative Damage in Athletes and Sedentary Young Man.
abstract: Sedentary lifestyle is highly associated with increased risk of cardiovascular disease, obesity, and type 2 diabetes. It is known that regular physical activity has positive effects on health; however several studies have shown that acute and strenuous exercise can induce oxidative stress and lead to DNA damage. As magnesium is essential in maintaining DNA integrity, the aim of this study was to determine whether four-week-long magnesium supplementation in students with sedentary lifestyle and rugby players could prevent or diminish impairment of DNA. By using the comet assay, our study demonstrated that the number of peripheral blood lymphocytes (PBL) with basal endogenous DNA damage is significantly higher in rugby players compared to students with sedentary lifestyle. On the other hand, magnesium supplementation significantly decreased the number of cells with high DNA damage, in the presence of exogenous H2O2, in PBL from both students and rugby players, and markedly reduced the number of cells with medium DNA damage in rugby players compared to corresponding control nonsupplemented group. Accordingly, the results of our study suggest that four-week-long magnesium supplementation has marked effects in protecting the DNA from oxidative damage in both rugby players and in young men with sedentary lifestyle. Clinical trial is registered at ANZCTR Trial Id: ACTRN12615001237572.
authors.affiliation: Department of Physiology, University of Belgrade, Faculty of Pharmacy, 11121 Belgrade, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Petrović
authors.affiliation: Department of Physiology, University of Belgrade, Faculty of Pharmacy, 11121 Belgrade, Serbia.
authors.forename: Dušanka
authors.initials: D
authors.lastname: Stanić
authors.affiliation: Department of Medical Biochemistry, University of Belgrade, Faculty of Pharmacy, 11121 Belgrade, Serbia.
authors.forename: Gordana
authors.initials: G
authors.lastname: Dmitrašinović
authors.affiliation: Department of Physiology, University of Belgrade, Faculty of Pharmacy, 11121 Belgrade, Serbia.
authors.forename: Bosiljka
authors.initials: B
authors.lastname: Plećaš-Solarović
authors.affiliation: Department of Medical Biochemistry, University of Belgrade, Faculty of Pharmacy, 11121 Belgrade, Serbia.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Ignjatović
authors.affiliation: Department of Physiology, University of Belgrade, Faculty of Pharmacy, 11121 Belgrade, Serbia.
authors.forename: Bojan
authors.initials: B
authors.lastname: Batinić
authors.affiliation: Department of Physiology, University of Belgrade, Faculty of Pharmacy, 11121 Belgrade, Serbia.
authors.forename: Dejana
authors.initials: D
authors.lastname: Popović
authors.affiliation: Department of Physiology, University of Belgrade, Faculty of Pharmacy, 11121 Belgrade, Serbia.
authors.forename: Vesna
authors.identifier: 0000-0002-1951-7291
authors.initials: V
authors.lastname: Pešić
mesh_terms: D000328:Adult; D056352:Athletes; D004249:DNA Damage / Q000187:drug effects*; D019587:Dietary Supplements; D005538:Football; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D057185:Sedentary Behavior; D055815:Young Adult
chemical_list: D006861:Hydrogen Peroxide; D008274:Magnesium