title: Detection of the Emerging Picornavirus Senecavirus A in Pigs, Mice, and Houseflies.
abstract: Senecavirus A (SVA) is an emerging picornavirus that has been recently associated with an increased number of outbreaks of vesicular disease and neonatal mortality in swine. Many aspects of SVA infection biology and epidemiology remain unknown. Here, we present a diagnostic investigation conducted in swine herds affected by vesicular disease and increased neonatal mortality. Clinical and environmental samples were collected from affected and unaffected herds and were screened for the presence of SVA by real-time reverse transcriptase PCR and virus isolation. Notably, SVA was detected and isolated from vesicular lesions and tissues of affected pigs, environmental samples, mouse feces, and mouse small intestine. SVA nucleic acid was also detected in houseflies collected from affected farms and from a farm with no history of vesicular disease. Detection of SVA in mice and housefly samples and recovery of viable virus from mouse feces and small intestine suggest that these pests may play a role on the epidemiology of SVA. These results provide important information that may allow the development of improved prevention and control strategies for SVA.
authors.affiliation: Animal Disease Research and Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, South Dakota, USA.
authors.forename: Lok R
authors.initials: LR
authors.lastname: Joshi
authors.affiliation: Animal Disease Research and Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, South Dakota, USA.
authors.forename: Kristin A
authors.initials: KA
authors.lastname: Mohr
authors.affiliation: Animal Disease Research and Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, South Dakota, USA.
authors.forename: Travis
authors.initials: T
authors.lastname: Clement
authors.affiliation: Animal Disease Research and Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, South Dakota, USA.
authors.forename: Kyle S
authors.initials: KS
authors.lastname: Hain
authors.affiliation: Pipestone Applied Research, Pipestone Veterinary Services, Pipestone, Minnesota, USA.
authors.forename: Bryan
authors.initials: B
authors.lastname: Myers
authors.affiliation: Pipestone Applied Research, Pipestone Veterinary Services, Pipestone, Minnesota, USA.
authors.forename: Joseph
authors.initials: J
authors.lastname: Yaros
authors.affiliation: Animal Disease Research and Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, South Dakota, USA.
authors.forename: Eric A
authors.initials: EA
authors.lastname: Nelson
authors.affiliation: Animal Disease Research and Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, South Dakota, USA.
authors.forename: Jane
authors.initials: J
authors.lastname: Christopher-Hennings
authors.affiliation: Embrapa Swine and Poultry, Concórdia, Santa Catarina, Brazil.
authors.forename: Danielle
authors.initials: D
authors.lastname: Gava
authors.affiliation: Embrapa Swine and Poultry, Concórdia, Santa Catarina, Brazil.
authors.forename: Rejane
authors.initials: R
authors.lastname: Schaefer
authors.affiliation: Embrapa Swine and Poultry, Concórdia, Santa Catarina, Brazil.
authors.forename: Luizinho
authors.initials: L
authors.lastname: Caron
authors.affiliation: Pipestone Applied Research, Pipestone Veterinary Services, Pipestone, Minnesota, USA.
authors.forename: Scott
authors.initials: S
authors.lastname: Dee
authors.affiliation: Animal Disease Research and Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, South Dakota, USA diego.diel@sdstate.edu.
authors.forename: Diego G
authors.initials: DG
authors.lastname: Diel
mesh_terms: D000818:Animals; D004196:Disease Outbreaks; D004783:Environmental Microbiology; D006793:Houseflies / Q000821:virology*; D051379:Mice / Q000821:virology*; D010849:Picornaviridae / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D013555:Swine Vesicular Disease / Q000453:epidemiology* / Q000821:virology*