title: Fine mapping and candidate gene analysis of the dominant glandless gene Gl 2 (e) in cotton (Gossypium spp.).
abstract: Dominant glandless gene Gl 2 (e) was fine-mapped to a 15 kb region containing one candidate gene encoding an MYC transcription factor, sequence and expression level of the gene were analyzed. Cottonseed product is an excellent source of oil and protein. However, this nutrition source is greatly limited in utilization by the toxic gossypol in pigment glands. It is reported that the Gl 2 (e) gene could effectively inhibit the formation of the pigment glands. Here, three F2 populations were constructed using two pairs of near isogenic lines (NILs), which differ nearly only by the gland trait, for fine mapping of Gl 2 (e) . DNA markers were identified from recently developed cotton genome sequence. The Gl 2 (e) gene was located within a 15-kb genomic interval between two markers CS2 and CS4 on chromosome 12. Only one gene was identified in the genomic interval as the candidate for Gl 2 (e) which encodes a family member of MYC transcription factor with 475-amino acids. Unexpectedly, the results of expression analysis indicated that the MYC gene expresses in glanded lines while almost does not express in glandless lines. These results suggest that the MYC gene probably serves as a vital positive regulator in the organogenesis pathway of pigment gland, and low expression of this gene will not launch the downstream pathway of pigment gland formation. This is the first pigment gland-related gene identification in cotton and will facilitate the research on glandless trait, cotton MYC proteins and low-gossypol cotton breeding.
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China.
authors.forename: Hailiang
authors.initials: H
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China.
authors.forename: Cairui
authors.initials: C
authors.lastname: Lu
authors.affiliation: USDA-ARS, Southern Plains Agricultural Research Center, Crop Germplasm Research Unit, 2881 F&B Road, College Station, TX, 77845, USA.
authors.forename: John Z
authors.initials: JZ
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China.
authors.forename: Changsong
authors.initials: C
authors.lastname: Zou
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China.
authors.forename: Youping
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China.
authors.forename: Qiaolian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China.
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China.
authors.forename: Wencui
authors.initials: W
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China. sglzms@163.com.
authors.forename: Guoli
authors.initials: G
authors.lastname: Song
mesh_terms: D002874:Chromosome Mapping; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005799:Genes, Dominant; D017343:Genes, Plant; D005819:Genetic Markers; D003368:Gossypium / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers; D010940:Plant Proteins; D016271:Proto-Oncogene Proteins c-myc; D014157:Transcription Factors