title: Evaluation of blood-brain barrier-stealth nanocomposites for in situ glioblastoma theranostics applications.
abstract: The blood-brain barrier (BBB) is a physiological structure of the blood vessels in the brain. The BBB efficiently traps most therapeutic drugs in the blood vessels and stops them from entering the brain tissue, resulting in a decreased therapeutic efficiency. In this study, we developed BBB-stealth nanocomposites composed of iron oxide (Fe3O4) nanoparticles (NPs) as a safe nanocarrier for glioblastoma therapy. We showed the antitumor activity of Dox/alg-Fe3O4 NPs using in vitro and in vivo tests. We demonstrated that G23-alg-Fe3O4 NPs crossed the BBB and entered the brain. In situ glioblastoma tumor-bearing mice were used to successfully evaluate the antitumor activity of G23-Dox/alg-Fe3O4 NPs. Magnetic resonance imaging (MRI) and bioluminescence imaging (BLI) confirmed the BBB crossing. The BBB-stealth nanocomposites show great potential for a proof-of-concept clinical trial as a theranostics platform for human brain tumor therapy.
authors.affiliation: Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 833, Taiwan and Department of Biomedical Imaging and Radiological Sciences, National Yang Ming University, Taipei 112, Taiwan.
authors.forename: Chia-Hao
authors.initials: CH
authors.lastname: Su
authors.affiliation: Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 833, Taiwan.
authors.forename: Ching-Yi
authors.initials: CY
authors.lastname: Tsai
authors.affiliation: Institute of Nuclear Physics, Polish Academy of Sciences, 152 Radzikowskiego, Krakow, Malopolska 31-342, Poland.
authors.forename: Boguslaw
authors.initials: B
authors.lastname: Tomanek
authors.affiliation: Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 833, Taiwan.
authors.forename: Wei-Yu
authors.initials: WY
authors.lastname: Chen
authors.affiliation: Institute of Oral Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, 1 University Road, Tainan City 701, Taiwan. FYCheng@mail.ncku.edu.tw.
authors.forename: Fong-Yu
authors.initials: FY
authors.lastname: Cheng
mesh_terms: D000464:Alginates / Q000737:chemistry; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage; D001812:Blood-Brain Barrier; D001932:Brain Neoplasms / Q000000981:diagnostic imaging / Q000188:drug therapy*; D004317:Doxorubicin / Q000008:administration & dosage; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry; D005909:Glioblastoma / Q000000981:diagnostic imaging / Q000188:drug therapy*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D051379:Mice; D053761:Nanocomposites / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D059906:Neuroimaging; D000068936:Theranostic Nanomedicine
chemical_list: D000464:Alginates; D000970:Antineoplastic Agents; D004337:Drug Carriers; D006603:Hexuronic Acids; D058185:Magnetite Nanoparticles; D004317:Doxorubicin; D020723:Glucuronic Acid