title: Ani s 11-Like Protein Is a Pepsin- and Heat-Resistant Major Allergen of Anisakis spp. and a Valuable Tool for Anisakis Allergy Component-Resolved Diagnosis.
abstract: BACKGROUND
  Anisakis simplex is a fish parasite responsible for gastrointestinal and allergic symptoms in humans. The Ani s 11-like protein has been proposed as an Anisakis allergen because its primary structure is similar to that of Ani s 11. The aims of this work were to analyse the frequency of detection of the Ani s 11-like protein and assess its diagnostic value.
  
  
  METHODS
  rAni s 11-like protein, rAni s 5 and rAni s 4 were expressed in Escherichia coli and rAni s 1 was produced in Pichia pastoris. Recombinant allergen detection patterns in 37 Anisakis-sensitised patients were determined. The stability to pepsin digestion and heat treatment of rAni s 11-like protein was also analysed by IgE immunoblotting.
  
  
  RESULTS
  Ani s 11-like protein is a major allergen detected by 78% of Anisakis-allergic patients, and 13.5% of patients detect only the rAni s 11-like allergen. This allergen is heat stable because it retains its capability of binding IgE after boiling for 30 min and it is resistant to pepsin digestion for 120 min.
  
  
  CONCLUSIONS
  These data indicate that the Ani s 11-like protein is a pepsin- and heat-resistant major allergen (Ani s 11.0201) of Anisakis spp. and a valuable tool for Anisakis allergy component-resolved diagnosis.
authors.affiliation: Department of Immunology, University Hospital La Paz Institute for Health Research (IdiPaz), Madrid, Spain.
authors.forename: Noelia
authors.initials: N
authors.lastname: Carballeda-Sangiao
authors.forename: Ana I
authors.initials: AI
authors.lastname: Rodríguez-Mahillo
authors.forename: Mercedes
authors.initials: M
authors.lastname: Careche
authors.forename: Alfonso
authors.initials: A
authors.lastname: Navas
authors.forename: Teresa
authors.initials: T
authors.lastname: Caballero
authors.forename: Javier
authors.initials: J
authors.lastname: Dominguez-Ortega
authors.forename: Jesús
authors.initials: J
authors.lastname: Jurado-Palomo
authors.forename: Miguel
authors.initials: M
authors.lastname: González-Muñoz
mesh_terms: D000328:Adult; D000368:Aged; D000485:Allergens / Q000276:immunology*; D000818:Animals; D017163:Anisakis / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology*; D016022:Case-Control Studies; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis* / Q000276:immunology*; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010434:Pepsin A / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D000485:Allergens; D000947:Antigens, Helminth; D011994:Recombinant Proteins; D007073:Immunoglobulin E; D010434:Pepsin A