title: Leaf Proteome Analysis Reveals Prospective Drought and Heat Stress Response Mechanisms in Soybean.
abstract: Drought and heat are among the major abiotic stresses that affect soybean crops worldwide. During the current investigation, the effect of drought, heat, and drought plus heat stresses was compared in the leaves of two soybean varieties, Surge and Davison, combining 2D-DIGE proteomic data with physiology and biochemical analyses. We demonstrated how 25 differentially expressed photosynthesis-related proteins affect RuBisCO regulation, electron transport, Calvin cycle, and carbon fixation during drought and heat stress. We also observed higher abundance of heat stress-induced EF-Tu protein in Surge. It is possible that EF-Tu might have activated heat tolerance mechanisms in the soybean. Higher level expressions of heat shock-related protein seem to be regulating the heat tolerance mechanisms. This study identifies the differential expression of various abiotic stress-responsive proteins that regulate various molecular processes and signaling cascades. One inevitable outcome from the biochemical and proteomics assays of this study is that increase of ROS levels during drought stress does not show significant changes at the phenotypic level in Davison and this seems to be due to a higher amount of carbonic anhydrase accumulation in the cell which aids the cell to become more resistant to cytotoxic concentrations of H2O2.
authors.affiliation: Department of Biology & Microbiology, South Dakota State University, Brookings, SD 57007, USA; Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77840, USA.
authors.forename: Aayudh
authors.initials: A
authors.lastname: Das
authors.affiliation: Department of Biology & Microbiology, South Dakota State University, Brookings, SD 57007, USA.
authors.forename: Moustafa
authors.initials: M
authors.lastname: Eldakak
authors.affiliation: Department of Biology & Microbiology, South Dakota State University, Brookings, SD 57007, USA.
authors.forename: Bimal
authors.initials: B
authors.lastname: Paudel
authors.affiliation: National Institute of Crop Science, Rural Development Administration (RDA), Wanju-gun, Jeollabuk-do 55365, Republic of Korea.
authors.forename: Dea-Wook
authors.initials: DW
authors.lastname: Kim
authors.affiliation: Department of Biology, California State University Northridge, Northridge, CA 91330, USA.
authors.forename: Homa
authors.initials: H
authors.lastname: Hemmati
authors.affiliation: Department of Biology, California State University Northridge, Northridge, CA 91330, USA.
authors.forename: Chhandak
authors.initials: C
authors.lastname: Basu
authors.affiliation: Department of Biology & Microbiology, South Dakota State University, Brookings, SD 57007, USA.
authors.forename: Jai S
authors.identifier: 0000-0002-4041-5804
authors.initials: JS
authors.lastname: Rohila
mesh_terms: D055864:Droughts; D018506:Gene Expression Regulation, Plant; D018869:Heat-Shock Response / Q000235:genetics*; D010788:Photosynthesis; D018515:Plant Leaves / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D020543:Proteome / Q000235:genetics*; D040901:Proteomics; D012273:Ribulose-Bisphosphate Carboxylase / Q000096:biosynthesis / Q000235:genetics; D013025:Glycine max / Q000235:genetics* / Q000502:physiology; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D010940:Plant Proteins; D020543:Proteome; D012273:Ribulose-Bisphosphate Carboxylase