title: COOLIA CANARIENSIS SP. NOV. (DINOPHYCEAE), A NEW NONTOXIC EPIPHYTIC BENTHIC DINOFLAGELLATE FROM THE CANARY ISLANDS(1).
abstract: A new photosynthetic dinoflagellate species, Coolia canariensis S. Fraga sp. nov., is described based on samples taken from tidal ponds on the rocky shore of the Canary Islands, northeast Atlantic Ocean. Its morphology was studied by LM and SEM. It is almost spherical and has a thick smooth theca with many scattered pores. Plate 1' is the biggest of the epithecal plates, and 7″ is twice as wide as it is long. Phylogeny inferred from the D1/D2 regions of the LSU nuclear rDNA of three strains of C. canariensis and several strains of other Coolia species, C. monotis, C. sp., showed that C. canariensis strains clustered in a well-supported clade distinct from the other species. No toxins were detected using mouse bioassay, liquid chromatography with Fluorescence detection (LC-FLD) or liquid chromatography-mass spectrometry (LC-MS). Its pigment composition is of the peridinin type of dinoflagellates. Together with this new species, many other strains of C. monotis from the Atlantic Ocean and Mediterranean Sea have been analyzed for toxin presence, and no evidence of toxin production related to yessotoxins (YTXs) was found, as was previously suggested for C. monotis from Australia.
authors.affiliation: Instituto Español de Oceanografía, Apdo. 1552, 36200 Vigo, SpainCentro Biologia Ambientale, University of Urbino, Vle. Trieste 296, 61100 Pesaro, ItalyCentro Biotecnologie, University of Urbino, Fano Ateneo, Via T. Campanella 1, 61032 Fano, ItalyInstituto de Investigacións Mariñas, Eduardo Cabello 6, 36208 Vigo, SpainCentro de Investigacións Mariñas, Consellería de Pesca e Asuntos Marítimos, Xunta de Galicia, Apdo. 13, 36260 Vilanova de Arousa, Spain.
authors.forename: Santiago
authors.initials: S
authors.lastname: Fraga
authors.affiliation: Instituto Español de Oceanografía, Apdo. 1552, 36200 Vigo, SpainCentro Biologia Ambientale, University of Urbino, Vle. Trieste 296, 61100 Pesaro, ItalyCentro Biotecnologie, University of Urbino, Fano Ateneo, Via T. Campanella 1, 61032 Fano, ItalyInstituto de Investigacións Mariñas, Eduardo Cabello 6, 36208 Vigo, SpainCentro de Investigacións Mariñas, Consellería de Pesca e Asuntos Marítimos, Xunta de Galicia, Apdo. 13, 36260 Vilanova de Arousa, Spain.
authors.forename: Antonella
authors.initials: A
authors.lastname: Penna
authors.affiliation: Instituto Español de Oceanografía, Apdo. 1552, 36200 Vigo, SpainCentro Biologia Ambientale, University of Urbino, Vle. Trieste 296, 61100 Pesaro, ItalyCentro Biotecnologie, University of Urbino, Fano Ateneo, Via T. Campanella 1, 61032 Fano, ItalyInstituto de Investigacións Mariñas, Eduardo Cabello 6, 36208 Vigo, SpainCentro de Investigacións Mariñas, Consellería de Pesca e Asuntos Marítimos, Xunta de Galicia, Apdo. 13, 36260 Vilanova de Arousa, Spain.
authors.forename: Irene
authors.initials: I
authors.lastname: Bianconi
authors.affiliation: Instituto Español de Oceanografía, Apdo. 1552, 36200 Vigo, SpainCentro Biologia Ambientale, University of Urbino, Vle. Trieste 296, 61100 Pesaro, ItalyCentro Biotecnologie, University of Urbino, Fano Ateneo, Via T. Campanella 1, 61032 Fano, ItalyInstituto de Investigacións Mariñas, Eduardo Cabello 6, 36208 Vigo, SpainCentro de Investigacións Mariñas, Consellería de Pesca e Asuntos Marítimos, Xunta de Galicia, Apdo. 13, 36260 Vilanova de Arousa, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Paz
authors.affiliation: Instituto Español de Oceanografía, Apdo. 1552, 36200 Vigo, SpainCentro Biologia Ambientale, University of Urbino, Vle. Trieste 296, 61100 Pesaro, ItalyCentro Biotecnologie, University of Urbino, Fano Ateneo, Via T. Campanella 1, 61032 Fano, ItalyInstituto de Investigacións Mariñas, Eduardo Cabello 6, 36208 Vigo, SpainCentro de Investigacións Mariñas, Consellería de Pesca e Asuntos Marítimos, Xunta de Galicia, Apdo. 13, 36260 Vilanova de Arousa, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Zapata
keywords: Coolia; LSU rRNA; cooliatoxin; dinoflagellate; peridinin; taxonomy; yessotoxin