title: Hck Promotes Neuronal Apoptosis Following Intracerebral Hemorrhage.
abstract: The hematopoietic cell kinase (Hck) is a member of the Src family protein kinases which regulates many signal transduction pathways including cell growth, proliferation, differentiation, migration, and apoptosis. However, the expression and function of Hck after intracerebral hemorrhage (ICH) are unknown. Western blot, immunohistochemistry, and immunofluorescence showed that Hck was obviously up-regulation in neurons adjacent to the hematoma after ICH. In addition, the temporary raise of Hck expression was paralleled with the expression of p53, Bax, and active caspase-3, suggesting that Hck was involved in neuronal apoptosis. Hck siRNA dramatically decrease hemin-induced expression of p53, Bax, and active caspase-3 as well as the amount of apoptotic SH-SY5Y cells in vitro. Furthermore, Hck interacted with p53. Hence, Hck might promote neuronal apoptosis via p53 signaling pathway after ICH.
authors.affiliation: Department of Geriatric Medicine, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Geriatric Medicine, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Rongrong
authors.initials: R
authors.lastname: Chen
authors.affiliation: Department of Pathogen Biology, Medical College, Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Shen
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Yaohua
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Yilu
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, 226001, Jiangsu, China. ntdxtaotao@163.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Tao
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China. shijiansheng2016@163.com.
authors.forename: Jiansheng
authors.initials: J
authors.lastname: Shi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D045744:Cell Line, Tumor; D002543:Cerebral Hemorrhage / Q000201:enzymology* / Q000473:pathology*; D006801:Humans; D008297:Male; D009474:Neurons / Q000201:enzymology* / Q000473:pathology*; D051572:Proto-Oncogene Proteins c-hck / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C496631:HCK protein, human; D051572:Proto-Oncogene Proteins c-hck
keywords: Hck; Intracerebral hemorrhage; Neuronal apoptosis