title: Automated Cell-Based Quantitation of 8-OHdG Damage.
abstract: Detection of 8-OHdG-base damage has been a big challenge for decades, though different analytical methods are developed. The recent approaches that are used for quantitating either the total amount of base damage or the amount of base damage per cell from different sources of samples are not automated. We have developed a method for automated damage detection from a single cell and applied it to 8-OHdG quantitation.
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, Ege University, Bornova, Izmir, Turkey.
authors.forename: Bilge
authors.initials: B
authors.lastname: Debelec-Butuner
authors.affiliation: Cancer Biology Laboratory, Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, Izmir, Turkey.
authors.forename: Aykut
authors.initials: A
authors.lastname: Bostancı
authors.affiliation: Medipan GmBH, Dahlewitz, Berlin, Germany.
authors.forename: Lisa
authors.initials: L
authors.lastname: Heiserich
authors.affiliation: Medipan GmBH, Dahlewitz, Berlin, Germany.
authors.forename: Caroline
authors.initials: C
authors.lastname: Eberle
authors.affiliation: Mass Spec. Laboratory, Department of Biochemistry, Faculty of Medicine, Akdeniz University, Antalya, Turkey.
authors.forename: Filiz
authors.initials: F
authors.lastname: Ozcan
authors.affiliation: Mass Spec. Laboratory, Department of Biochemistry, Faculty of Medicine, Akdeniz University, Antalya, Turkey.
authors.forename: Mutay
authors.initials: M
authors.lastname: Aslan
authors.affiliation: Medipan GmBH, Dahlewitz, Berlin, Germany.|Faculty of Science, Brandenburg University of Technology Cottbus-Senftenberg, Senftenberg, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Roggenbuck
authors.affiliation: Cancer Biology Laboratory, Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, Izmir, Turkey. ks_korkmaz@yahoo.com.
authors.forename: Kemal Sami
authors.initials: KS
authors.lastname: Korkmaz
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D004249:DNA Damage / Q000187:drug effects* / Q000235:genetics; D004260:DNA Repair / Q000235:genetics; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D018384:Oxidative Stress / Q000235:genetics; D059010:Single-Cell Analysis / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine
keywords: 8-OHdG; Base excision repair; DNA damage; Oxidative stress; ROS