title: Microbial mechanisms of using enhanced ecological floating beds for eutrophic water improvement.
abstract: Enhanced ecological floating beds were implemented to reduce nutrient quantity and improve the water quality of a eutrophic lake. The results showed that average removal efficiencies of CODCr, total nitrogen, NH3-N and total phosphorus for Canna indica L. set-up were 23.1%, 15.3%, 18.1% and 19.4% higher, respectively, than that of the setup with only substrate, and 14.2%, 12.8%, 7.9% and 11.9% higher than Iris pseudacorus L. ecological floating bed. The microbial community structure had obvious differences between devices and low similarity; bacteria were mainly attached on the fiber filling. The microbial population was abundant at the start and end of the experiment. Shannon index of samples selected ranged from 0.85 to 1.05. The sequencing results showed that fiber filling collected most uncultured bacteria species and the majority of bacteria on the plant roots were β-Proteobacteria and α-Proteobacteria. The co-dominant species attaching to the filling and plant was Nitrosomonadaceae.
authors.affiliation: School of Environmental Science and Engineering, Tianjin University, 300072, China; Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard, Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wu
authors.affiliation: School of Environmental Science and Engineering, Tianjin University, 300072, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Hu
authors.affiliation: School of Environmental Science and Engineering, Tianjin University, 300072, China.
authors.forename: Shuqun
authors.initials: S
authors.lastname: Li
authors.affiliation: School of Environmental Science and Engineering, Tianjin University, 300072, China. Electronic address: pengsen@tju.edu.cn.
authors.forename: Sen
authors.initials: S
authors.lastname: Peng
authors.affiliation: Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard, Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China.
authors.forename: Huabing
authors.initials: H
authors.lastname: Zhao
mesh_terms: D044822:Biodiversity; D057919:Biological Oxygen Demand Analysis; D058645:Denaturing Gradient Gel Electrophoresis; D004463:Ecology; D005068:Eutrophication; D060106:Lakes; D009584:Nitrogen / Q000302:isolation & purification; D010758:Phosphorus / Q000302:isolation & purification; D010802:Phylogeny; D063245:Plant Development; D016133:Polymerase Chain Reaction; D020560:Proteobacteria / Q000378:metabolism*; D014867:Water / Q000737:chemistry; D018508:Water Purification / Q000295:instrumentation* / Q000379:methods*
chemical_list: D014867:Water; D010758:Phosphorus; D009584:Nitrogen
keywords: Bacterial diversity; Enhanced ecological floating bed; Eutrophic water body; PCR–DGGE