title: Dynamic thiol/disulfide homeostasis in patients with autoimmune subclinical hypothyroidism.
abstract: OBJECTIVE
  The aim of this study was to investigate dynamic thiol/disulfide homeostasis in autoimmune subclinical hypothyroidism.
  
  
  METHODS
  Forty-eight patients with a new diagnosis of subclinical hypothyroidism due to Hashimoto thyroiditis who were not yet under medical therapy, and 48 healthy control subjects were enrolled. Thiol/disulfide homeostasis [native thiol-disulfide exchanges] was measured in both groups using the automated method developed by Erel and Neselioglu. An absolute difference of 0.5 between the total thiol and native thiol concentrations revealed the disulfide bond amount.
  
  
  RESULTS
  The native thiol level (p = 0.014) and native thiol/total thiol ratio (p = 0.001) were lower in patients with subclinical hypothyroidism than in the control group. Meanwhile, the disulfide level (p = 0.004), disulfide/native thiol ratio (p = 0.001), and disulfide/total thiol (p = 0.001) ratio were higher in patients with subclinical hypothyroidism than in the control group. The antithyroid peroxidase and anti-thyroglobulin levels were positively correlated with the disulfide/native thiol ratio (r = 0.339, p = 0.019; r = 0.243, p = 0.023, respectively) and the disulfide/total thiol ratio (r = 0.133, p = 0.019; r = 0.238, p = 0.026, respectively) and negatively correlated with the native thiol/total thiol ratio (r = -0.292, p = 0.004; r = -0.233, p = 0.022, respectively).
  
  
  CONCLUSIONS
  We found that thiol/disulfide homeostasis shifted to disulfide formation in patients with subclinical hypothyroidism and that thyroid autoantibodies were positively correlated with thiol oxidation. It is not clear whether abnormal thiol/disulfide homeostasis is a cause or a consequence in Hashimoto thyroiditis. Further studies are required.
authors.affiliation: a Department of Internal Medicine , Ankara Numune Training and Research Hospital , Ankara , Turkey.
authors.forename: Ihsan
authors.initials: I
authors.lastname: Ates
authors.affiliation: a Department of Internal Medicine , Ankara Numune Training and Research Hospital , Ankara , Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Altay
authors.affiliation: b Department of Biochemistry , Ankara Numune Training and Research Hospital , Ankara , Turkey.|c Department of Biochemistry , Yıldırım Beyazıt University Medical Faculty , Ankara , Turkey.
authors.forename: Fatma Meric
authors.initials: FM
authors.lastname: Yilmaz
authors.affiliation: b Department of Biochemistry , Ankara Numune Training and Research Hospital , Ankara , Turkey.
authors.forename: Canan
authors.initials: C
authors.lastname: Topcuoglu
authors.affiliation: c Department of Biochemistry , Yıldırım Beyazıt University Medical Faculty , Ankara , Turkey.
authors.forename: Salim
authors.initials: S
authors.lastname: Neselioglu
authors.affiliation: c Department of Biochemistry , Yıldırım Beyazıt University Medical Faculty , Ankara , Turkey.
authors.forename: Ozcan
authors.initials: O
authors.lastname: Erel
authors.affiliation: a Department of Internal Medicine , Ankara Numune Training and Research Hospital , Ankara , Turkey.
authors.forename: Nisbet
authors.initials: N
authors.lastname: Yilmaz
mesh_terms: D000328:Adult; D004220:Disulfides / Q000097:blood*; D005260:Female; D050031:Hashimoto Disease / Q000097:blood*; D006706:Homeostasis / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D013438:Sulfhydryl Compounds / Q000097:blood*
chemical_list: D004220:Disulfides; D013438:Sulfhydryl Compounds
keywords: Hashimoto thyroiditis; oxidative stress; thiol oxidation; thiol/disulfide homeostasis