title: Fast, Efficient, and Gentle Transfection of Human Adherent Cells in Suspension.
abstract: We demonstrate a highly efficient method for gene delivery into clinically relevant human cell types, such as induced pluripotent stem cells (iPSCs) and fibroblasts, reducing the protocol time by one full day. To preserve cell physiology during gene transfer, we designed a microfluidic strategy, which facilitates significant gene delivery in a short transfection time (<1 min) for several human cell types. This fast, optimized and generally applicable cell transfection method can be used for rapid screening of different delivery systems and has significant potential for high-throughput cell therapy applications.
authors.affiliation: Department of Chemical Engineering and Material Science, University of Minnesota , Minneapolis, Minnesota 55455, United States.
authors.forename: Pranav
authors.initials: P
authors.lastname: Agrawal
authors.affiliation: Department of Chemistry, University of Minnesota , Minneapolis, Minnesota 55455, United States.
authors.forename: Nilesh P
authors.initials: NP
authors.lastname: Ingle
authors.affiliation: Department of Chemistry, University of Minnesota , Minneapolis, Minnesota 55455, United States.
authors.forename: William S
authors.initials: WS
authors.lastname: Boyle
authors.affiliation: Stem Cell Institute and Division of Pediatric Blood and Marrow Transplantation, University of Minnesota , Minneapolis, Minnesota 55455, United States.
authors.forename: Emily
authors.initials: E
authors.lastname: Ward
authors.affiliation: Stem Cell Institute and Division of Pediatric Blood and Marrow Transplantation, University of Minnesota , Minneapolis, Minnesota 55455, United States.
authors.forename: Jakub
authors.initials: J
authors.lastname: Tolar
authors.affiliation: Department of Chemical Engineering and Material Science, University of Minnesota , Minneapolis, Minnesota 55455, United States.
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Dorfman
authors.affiliation: Department of Chemistry, University of Minnesota , Minneapolis, Minnesota 55455, United States.
authors.forename: Theresa M
authors.initials: TM
authors.lastname: Reineke
mesh_terms: D002454:Cell Differentiation / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology; D005434:Flow Cytometry; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D014162:Transfection / Q000379:methods*
keywords: fibroblasts; flow cytometry; microfluidics; pluripotent stem cells; suspension transfection