title: Enhancement of fructosyltransferase and fructooligosaccharides production by A. oryzae DIA-MF in Solid-State Fermentation using aguamiel as culture medium.
abstract: The aim of this work was to improve the production of fructosyltransferase (FTase) by Solid-State Fermentation (SSF) using aguamiel (agave sap) as culture medium and Aspergillus oryzae DIA-MF as producer strain. SSF was carried out evaluating the following parameters: inoculum rate, incubation temperature, initial pH and packing density to determine the most significant factors through Box-Hunter and Hunter design. The significant factors were then further optimized using a Box-Behnken design and response surface methodology. The maximum FTase activity (1347U/L) was obtained at 32°C, using packing density of 0.7g/cm(3). Inoculum rate and initial pH had no significant influence on the response. FOS synthesis applying the enzyme produced by A. oryzae DIA-MF was also studied using aguamiel as substrate.
authors.affiliation: Group of Bioprocesses, Food Research Department, School of Chemistry, University Autonomous of Coahuila, 25280 Saltillo, Coahuila, Mexico.
authors.forename: Diana B
authors.initials: DB
authors.lastname: Muñiz-Márquez
authors.affiliation: Group of Bioprocesses, Food Research Department, School of Chemistry, University Autonomous of Coahuila, 25280 Saltillo, Coahuila, Mexico.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Contreras
authors.affiliation: Group of Bioprocesses, Food Research Department, School of Chemistry, University Autonomous of Coahuila, 25280 Saltillo, Coahuila, Mexico.
authors.forename: Raúl
authors.initials: R
authors.lastname: Rodríguez
authors.affiliation: Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, Netherlands.
authors.forename: Solange I
authors.initials: SI
authors.lastname: Mussatto
authors.affiliation: IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal.
authors.forename: José A
authors.initials: JA
authors.lastname: Teixeira
authors.affiliation: Group of Bioprocesses, Food Research Department, School of Chemistry, University Autonomous of Coahuila, 25280 Saltillo, Coahuila, Mexico. Electronic address: cristobal.aguilar@uadec.edu.mx.
authors.forename: Cristóbal N
authors.initials: CN
authors.lastname: Aguilar
mesh_terms: D001236:Aspergillus oryzae / Q000201:enzymology / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D003470:Culture Media; D005285:Fermentation; D006602:Hexosyltransferases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009844:Oligosaccharides / Q000096:biosynthesis*; D013696:Temperature; D014866:Waste Products; D001426:Bacterial Proteins
chemical_list: D003470:Culture Media; D006602:Hexosyltransferases; D009844:Oligosaccharides; D014866:Waste Products; C116580:fructooligosaccharide; C021451:inulosucrase; D001426:Bacterial Proteins
keywords: Aguamiel; Fructooligosaccharides; Fructosyltransferase; Solid State Fermentation