title: Changes of the plasma metabolome of newly born piglets subjected to postnatal hypoxia and resuscitation with air.
abstract: Perinatal hypoxic-ischemic brain damage is a major cause of mortality and morbidity in the neonatal period. Currently, limited ranges of biochemical tests assessing the intensity and duration of hypoxia are ready for clinical use. However, the need to initiate hypothermia therapy early after the clinical suspicion of hypoxic-ischemic encephalopathy requires the availability of early and reliable hypoxia markers. We have sought these biomarkers in an experimental model of hypoxia reoxygenation.
  
  
  
  Hypoxia and hypotension were induced in newborn piglets following a standardized model and reoxygenation was carried out using room air (RA). An untargeted liquid chromatography-time of flight mass spectrometry (LC-TOFMS) approach was used to assess changes in the metabolomic profile of plasma samples after intense hypoxia and upon reoxygenation.
  
  
  
  At the end of hypoxia, the plasma metabolome showed an increased plasma concentration of analytes reflecting a metabolic adaptation to prolonged anaerobiosis. However, after resuscitation, metabolite levels returned to the starting values.
  
  
  
  Severe hypoxia induces early, significant, and transient changes of specific metabolites in the plasma metabolome, which represent a snapshot of the biochemical adaptation of mammals to intense hypoxia. These metabolites could have applicability in predicting the severity of hypoxia in the clinical setting.
authors.affiliation: Department of Pediatric Research, Institute for Surgical Research, University of Oslo, Oslo University Hospital - Rikshospitalet, Oslo, Norway.
authors.forename: Rønnaug
authors.initials: R
authors.lastname: Solberg
authors.affiliation: Neonatal Research Group, Health Research Institute Hospital La Fe, Valencia, Spain.
authors.forename: Julia
authors.initials: J
authors.lastname: Kuligowski
authors.affiliation: Department of Pediatric Research, Institute for Surgical Research, University of Oslo, Oslo University Hospital - Rikshospitalet, Oslo, Norway.
authors.forename: Leonid
authors.initials: L
authors.lastname: Pankratov
authors.affiliation: Neonatal Research Group, Health Research Institute Hospital La Fe, Valencia, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Escobar
authors.affiliation: Leitat Technological Center, Safety and Sustainability Division, Valencia, Spain.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Quintás
authors.affiliation: Neonatal Research Group, Health Research Institute Hospital La Fe, Valencia, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Lliso
authors.affiliation: Neonatal Research Group, Health Research Institute Hospital La Fe, Valencia, Spain.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Sánchez-Illana
authors.affiliation: Department of Pediatric Research, Institute for Surgical Research, University of Oslo, Oslo University Hospital - Rikshospitalet, Oslo, Norway.
authors.forename: Ola Didrik
authors.initials: OD
authors.lastname: Saugstad
authors.affiliation: Neonatal Research Group, Health Research Institute Hospital La Fe, Valencia, Spain.|Division of Neonatology, University & Polytechnic Hospital La Fe, Valencia, Spain.
authors.forename: Máximo
authors.identifier: 0000-0003-0061-4742
authors.initials: M
authors.lastname: Vento
mesh_terms: D000388:Air; D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000378:metabolism; D002138:Calibration; D002853:Chromatography, Liquid; D005260:Female; D007035:Hypothermia / Q000473:pathology; D000860:Hypoxia / Q000097:blood* / Q000473:pathology; D007511:Ischemia / Q000473:pathology; D008297:Male; D055442:Metabolome; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism; D011897:Random Allocation; D012151:Resuscitation / Q000379:methods*; D013552:Swine
chemical_list: D015415:Biomarkers; D010100:Oxygen