title: Low-dose oral interferon modulates expression of inflammatory and autoimmune genes in cattle.
abstract: While the safety and efficacy profiles of orally administered bovine interferon (IFN) alpha have been documented, the mechanism(s) that result in clinical benefits remain elusive. One approach to delineating the molecular pathways of IFN efficacy is through the use of gene expression profiling technologies. In this proof-of-concept study, different (0, 50, 200 and 800 units) oral doses of natural bovine IFN (type I) were tested in cattle to determine if oral IFN altered the expression of genes that may be pivotal to the development of systemic resistance to viral infections such as foot-and-mouth disease (FMD). Oral IFN was administered twice: Time 0 and 8h later. Blood was collected at 0, 8 and 24h after the first IFN administration, and DNA isolated from peripheral blood mononuclear cells (PBMCs) was employed in quantitative polymerase chain reaction (qPCR) microarray assays. Within 8h, 50 and 200 units of oral IFN induced significant (P<0.05) changes in expression of 41 of 92 tested autoimmune and inflammatory response-associated genes. These data suggest that orally administered IFN is a viable approach for providing short-term antiviral immunity to livestock exposed to viruses such as FMD virus (FMDV) until such a time that an effective vaccine can be produced and distributed to producers.
authors.affiliation: Beech Tree Labs, 117 Chapman Street, Providence, RI 02905, United States.
authors.forename: Stephen W
authors.initials: SW
authors.lastname: Mamber
authors.affiliation: Beech Tree Labs, 117 Chapman Street, Providence, RI 02905, United States.
authors.forename: Jeremy
authors.initials: J
authors.lastname: Lins
authors.affiliation: Beech Tree Labs, 117 Chapman Street, Providence, RI 02905, United States.
authors.forename: Volkan
authors.initials: V
authors.lastname: Gurel
authors.affiliation: Animal Agricultural Consulting International, 63 Neches Court, Scroggins, TX 75480, United States.
authors.forename: David P
authors.initials: DP
authors.lastname: Hutcheson
authors.affiliation: Texas A&M AgriLife Research Extension Center, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University System, Amarillo, TX 79106, United States.
authors.forename: Pablo
authors.initials: P
authors.lastname: Pinedo
authors.affiliation: Agri Research Center, 16851 Hope Road, Canyon, TX 79015, United States.
authors.forename: David
authors.initials: D
authors.lastname: Bechtol
authors.affiliation: Department of Veterinary Sciences, Ohio State University, Columbus, OH 43210, United States.
authors.forename: Steven
authors.initials: S
authors.lastname: Krakowka
authors.affiliation: Colorado Serum Company, 4950 York Street, Denver, CO 80216, United States.
authors.forename: Rachel
authors.initials: R
authors.lastname: Fields-Henderson
authors.affiliation: Bomunity Ltd., Co., 400 W. Walnut Street, Hereford, TX 79045, United States. Electronic address: shadynookfarms@hotmail.com.
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Cummins
mesh_terms: D000818:Animals; D015551:Autoimmunity / Q000187:drug effects* / Q000235:genetics; D002417:Cattle; D003402:Creatine Kinase / Q000097:blood; D016207:Cytokines / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects*; D016898:Interferon-alpha / Q000008:administration & dosage / Q000627:therapeutic use*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D008297:Male; D018121:Receptors, Cytokine / Q000235:genetics
chemical_list: D016207:Cytokines; D016898:Interferon-alpha; D018121:Receptors, Cytokine; D003402:Creatine Kinase
keywords: Autoimmune and inflammatory response genes; Cattle-bovine; Foot-and-mouth disease (FMD); IFN–stimulated genes (ISGs); Interferon (IFN) alpha (α); KEGG pathway analysis