title: Aging-associated oxidized albumin promotes cellular senescence and endothelial damage.
abstract: Increased levels of oxidized proteins with aging have been considered a cardiovascular risk factor. However, it is unclear whether oxidized albumin, which is the most abundant serum protein, induces endothelial damage. The results of this study indicated that with aging processes, the levels of oxidized proteins as well as endothelial microparticles release increased, a novel marker of endothelial damage. Among these, oxidized albumin seems to play a principal role. Through in vitro studies, endothelial cells cultured with oxidized albumin exhibited an increment of endothelial damage markers such as adhesion molecules and apoptosis levels. In addition, albumin oxidation increased the amount of endothelial microparticles that were released. Moreover, endothelial cells with increased oxidative stress undergo senescence. In addition, endothelial cells cultured with oxidized albumin shown a reduction in endothelial cell migration measured by wound healing. As a result, we provide the first evidence that oxidized albumin induces endothelial injury which then contributes to the increase of cardiovascular disease in the elderly subjects.
authors.affiliation: Nephrology Unit, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Reina Sofía University Hospital, Córdoba, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Luna
authors.affiliation: Department of Systems Biology, Physiology Unit, Universidad de Alcalá, Madrid, Spain.
authors.forename: Matilde
authors.initials: M
authors.lastname: Alique
authors.affiliation: Department of Systems Biology, Physiology Unit, Universidad de Alcalá, Madrid, Spain.
authors.forename: Estefanía
authors.initials: E
authors.lastname: Navalmoral
authors.affiliation: Anesthesia Unit, Reina sofía University Hospital, Córdoba, Spain.
authors.forename: Maria-Victoria
authors.initials: MV
authors.lastname: Noci
authors.affiliation: Department of Systems Biology, Physiology Unit, Universidad de Alcalá, Madrid, Spain.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Bohorquez-Magro
authors.affiliation: Nephrology Unit, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Reina Sofía University Hospital, Córdoba, Spain.
authors.forename: Julia
authors.initials: J
authors.lastname: Carracedo
authors.affiliation: Department of Systems Biology, Physiology Unit, Universidad de Alcalá, Madrid, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Ramírez
mesh_terms: D000328:Adult; D062107:Advanced Oxidation Protein Products / Q000097:blood*; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000097:blood*; D017209:Apoptosis; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002478:Cells, Cultured; D016922:Cellular Senescence; D042783:Endothelial Cells / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D012307:Risk Factors; D012709:Serum Albumin / Q000502:physiology*
chemical_list: D062107:Advanced Oxidation Protein Products; D015415:Biomarkers; D012709:Serum Albumin
keywords: elderly; microparticles; oxidative stress; vascular damage