title: Nanoparticle Effects on Human Platelets in Vitro: A Comparison between PAMAM and Triazine Dendrimers.
abstract: Triazine and PAMAM dendrimers of similar size and number of cationic surface groups were compared for their ability to promote platelet aggregation. Triazine dendrimers (G3, G5 and G7) varied in molecular weight from 8 kDa-130 kDa and in surface groups 16-256. PAMAM dendrimers selected for comparison included G3 (7 kDa, 32 surface groups) and G6 (58 kDa, 256 surface groups). The treatment of human platelet-rich plasma (PRP) with low generation triazine dendrimers (0.01-1 µM) did not show any significant effect in human platelet aggregation in vitro; however, the treatment of PRP with larger generations promotes an effective aggregation. These results are in agreement with studies performed with PAMAM dendrimers, where large generations promote aggregation. Triazine dendrimers promote aggregation less aggressively than PAMAM dendrimers, a factor attributed to differences in cationic charge or the formation of supramolecular assemblies of dendrimers.
authors.affiliation: Department of Chemistry &amp; Biochemistry, Texas Christian University, Fort Worth, TX 76129, USA. a.encisobarros@tcu.edu.
authors.forename: Alan E
authors.initials: AE
authors.lastname: Enciso
authors.affiliation: Nanotechnology Characterization Lab, Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USA. neunb@mail.nih.gov.
authors.forename: Barry
authors.initials: B
authors.lastname: Neun
authors.affiliation: Nanotechnology Characterization Lab, Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USA. rodriguezjc@mail.nih.gov.
authors.forename: Jamie
authors.initials: J
authors.lastname: Rodriguez
authors.affiliation: Department of Molecular and Medical Genetics &amp; Institute of Cancer Research, University of North Texas Health Science Center, Fort Worth, TX 76109, USA. amalendu.ranjan@unthsc.edu.
authors.forename: Amalendu P
authors.initials: AP
authors.lastname: Ranjan
authors.affiliation: Nanotechnology Characterization Lab, Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USA. marina@mail.nih.gov.
authors.forename: Marina A
authors.initials: MA
authors.lastname: Dobrovolskaia
authors.affiliation: Department of Chemistry &amp; Biochemistry, Texas Christian University, Fort Worth, TX 76129, USA. e.simanek@tcu.edu.
authors.forename: Eric E
authors.initials: EE
authors.lastname: Simanek
mesh_terms: D001792:Blood Platelets / Q000187:drug effects*; D050091:Dendrimers / Q000494:pharmacology; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D010974:Platelet Aggregation / Q000187:drug effects*; D014227:Triazines / Q000494:pharmacology*
chemical_list: D050091:Dendrimers; C104700:PAMAM Starburst; D014227:Triazines
keywords: PAMAM; biocompatibility; dendrimer; platelet; triazine