title: Study of cytogenetic abnormalities in G-CSF stimulated peripheral blood cells and non-stimulated bone marrow cells of patients with myelofibrosis.
abstract: The aim of the study was to improve cytogenetic diagnostics and monitoring of myelofibrosis and to reveal the spectrum of cytogenetic abnormalities in patients from Ukraine.
  
  
  METHODS
  A total of 42 patients (23 females and 19 males) with myelofibrosis was studied using different cytogenetic methods. Granulocyte colony-stimulating factor (G-CSF) was added by the new method during cultivation of peripheral blood (PB) cells from 31 patients for specific stimulation of mitotic divisions. Two patients underwent examination by fluorescent in situ hybridization method.
  
  
  RESULTS
  In cell cultures of PB stimulated in vitro with G-CSF and in non-stimulated bone marrow chromosome abnormalities were found in 19 (45.2%) of all the patients. The spectrum of cytogenetic abnormalities of bone marrow and PB was the same in all of the patients. Aspiration of bone marrow was unsuccessful due to significant fibrosis in 10 (29.4%) of 34 patients. The study by fluorescent in situ hybridization method confirmed cytogenetic abnormalities revealed by G-method and discovered additional possibly normal subclone.
  
  
  CONCLUSIONS
  Cytogenetic study of PB using in vitro G-CSF as a specific stimulant of mitosis instead of phytohemagglutinin revealed significant variety of chromosomal abnormalities in Ukrainian patients with myelofibrosis. This method could be a less invasive alternative to cytogenetic examination of bone marrow in the subgroup of patients with considerable fibrosis and consecutive changes. The usage of fluorescent in situ hybridization method supplemented karyotyping by G-banding method.
authors.affiliation: State Institution "Institute of Blood Pathology and Transfusion Medicine of National Academy of Medical Sciences of Ukraine", Lviv 79044, Ukraine.
authors.forename: R Y
authors.initials: RY
authors.lastname: Lozynskyy
authors.affiliation: State Institution "Institute of Hereditary Pathology of National Academy of Medical Sciences of Ukraine", Lviv 79000, Ukraine.
authors.forename: M R
authors.initials: MR
authors.lastname: Lozynska
authors.affiliation: Medical Center "IGR", Kyiv 03115, Ukraine.
authors.forename: Y V
authors.initials: YV
authors.lastname: Hontar
authors.affiliation: State Institution "Institute of Hereditary Pathology of National Academy of Medical Sciences of Ukraine", Lviv 79000, Ukraine.
authors.forename: N L
authors.initials: NL
authors.lastname: Huleyuk
authors.affiliation: State Institution "Institute of Blood Pathology and Transfusion Medicine of National Academy of Medical Sciences of Ukraine", Lviv 79044, Ukraine.
authors.forename: Z V
authors.initials: ZV
authors.lastname: Maslyak
authors.affiliation: State Institution "Institute of Blood Pathology and Transfusion Medicine of National Academy of Medical Sciences of Ukraine", Lviv 79044, Ukraine.
authors.forename: V L
authors.initials: VL
authors.lastname: Novak
mesh_terms: D001773:Blood Cells / Q000378:metabolism / Q000473:pathology*; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology*; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D020732:Cytogenetic Analysis / Q000379:methods*; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D055728:Primary Myelofibrosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D016179:Granulocyte Colony-Stimulating Factor