title: Differential expression of microRNAs in plasma of patients with prediabetes and newly diagnosed type 2 diabetes.
abstract: OBJECTIVE
  MicroRNAs (miRNAs) are present in plasma and have emerged as critical regulators of gene expression at posttranscriptional level, and thus are involved in various human diseases, including diabetes. The objective of this study was to screen and validate differentially expressed plasma miRNAs in prediabetes and newly diagnosed type 2 diabetes (T2D).
  
  
  METHODS
  In this study, we screened differentially expressed plasma miRNAs in prediabetes and newly diagnosed T2D by miRNA microarray analysis, and validated the expression of candidate miRNAs using quantitative reverse transcription polymerase chain reaction assays. Furthermore, we performed gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway analyses to disclose functional enrichment of genes predicted to be regulated by the differentially expressed miRNAs.
  
  
  RESULTS
  Notably, our results revealed that hsa-miR-1249, hsa-miR-320b, and hsa-miR-572 (P < 0.05) were differentially expressed among the three groups, which yielded an area under the receiver operator characteristics curve (AUC) of 0.784 [95 % confidence interval (CI) 0.685-0.883], 0.946 (95 % CI 0.906-0.985), and 0.843 (95 % CI 0.766-0.920) discriminating T2D patients from NGT control groups, respectively, while the AUC was 0.887 (95 % CI 0.818-0.957), 0.635 (95 % CI 0.525-0.744), and 0.69 (95 % CI 0.580-0.793) discriminating prediabetes patients from NGT control groups, respectively. In addition, GO and KEGG pathway analyses showed that genes predicted to be regulated by differentially expressed miRNAs were significantly enriched in several related biological processes and pathways, including the development of multicellular organisms, signal transduction, cell differentiation, apoptosis, cell metabolism, ion transport regulation, and other biological functions.
  
  
  CONCLUSIONS
  Taken together, our results showed differentially expressed miRNAs in T2D and prediabetes. Plasma hsa-miR-1249, hsa-miR-320b, and hsa-miR-572 may serve as novel biomarkers for diagnosis and potential targets for the treatment for prediabetes and T2D.
authors.affiliation: Department of Medical Laboratory, Guizhou Medical University, Guiyang, Guizhou Province, People's Republic of China.
authors.forename: Shaoying
authors.initials: S
authors.lastname: Yan
authors.affiliation: Department of Clinical Laboratory, People's Hospital of Guizhou Province, Guiyang, Guizhou Province, People's Republic of China.
authors.forename: Tianqiong
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, People's Hospital of Guizhou Province, Guiyang, Guizhou Province, People's Republic of China.
authors.forename: Shengwen
authors.initials: S
authors.lastname: Huang
authors.affiliation: Department of Clinical Laboratory, People's Hospital of Guizhou Province, Guiyang, Guizhou Province, People's Republic of China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Di
authors.affiliation: Department of Medical Laboratory, Guizhou Medical University, Guiyang, Guizhou Province, People's Republic of China.
authors.forename: Yunzhu
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Clinical Laboratory, People's Hospital of Guizhou Province, Guiyang, Guizhou Province, People's Republic of China.
authors.forename: Xingmei
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Clinical Laboratory, People's Hospital of Guizhou Province, Guiyang, Guizhou Province, People's Republic of China.
authors.forename: Zhenyuan
authors.initials: Z
authors.lastname: Luo
authors.affiliation: Department of Medical Laboratory, Guizhou Medical University, Guiyang, Guizhou Province, People's Republic of China.
authors.forename: Wenping
authors.initials: W
authors.lastname: Han
authors.affiliation: Department of Clinical Laboratory, People's Hospital of Guizhou Province, Guiyang, Guizhou Province, People's Republic of China. anhonggao@sina.com.
authors.forename: Bangquan
authors.identifier: http://orcid.org/0000-0002-6901-4326
authors.initials: B
authors.lastname: An
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D011236:Prediabetic State / Q000097:blood*
chemical_list: D015415:Biomarkers; D035683:MicroRNAs
keywords: Differential expression; MicroRNA; Plasma; Prediabetes; Type 2 diabetes (T2D)