title: Serotyping and Antimicrobial Susceptibility Pattern of Escherichia coli Isolates from Urinary Tract Infections in Pediatric Population in a Tertiary Care Hospital.
abstract: Urinary tract infections (UTIs) in pediatric population are associated with high morbidity and long term complications. In recent years, there is increased prevalence of Escherichia coli (E. coli) strains producing extended spectrum β-lactamase, Amp C, and Metallo β-lactamase, making the clinical management even more difficult. This study was aimed to detect the serotypes and to determine antimicrobial susceptibility profile of E. coli isolates from urine samples of children <10 yrs old. A total of 75 pure E. coli strains isolated from patients with symptoms of UTI and colony count ≥10(5) organisms/mL were included in the study. Antibiotic sensitivity pattern showed maximum resistance to nalidixic acid (98.7%), followed by ampicillin (97.3%), amoxi-clavulanate (96%), and fluoroquinolones (92%) while most of the isolates were found sensitive to piperacillin-tazobactam (13.3%), nitrofurantoin (5.3%), and meropenem (1.3%). 48% of the strains were ESBL producer (extended spectrum beta lactamase). 44% strains were typable withantisera used in our study and the most common serogroup was O6 (33.3%) followed by O1 (15.1%) and O15 (15.1%). To conclude, judicious use of antibiotics according to hospital antibiotic policy and infection control measures should be implemented to prevent spread of multidrug resistant organisms.
authors.affiliation: Department of Microbiology, PGIMER and Dr. RML Hospital, New Delhi 110001, India.
authors.forename: Shweta
authors.identifier: 0000-0001-8221-9177
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Department of Microbiology, PGIMER and Dr. RML Hospital, New Delhi 110001, India.
authors.forename: Nirmaljit
authors.initials: N
authors.lastname: Kaur
authors.affiliation: Department of Microbiology, PGIMER and Dr. RML Hospital, New Delhi 110001, India.
authors.forename: Shalini
authors.initials: S
authors.lastname: Malhotra
authors.affiliation: Department of Microbiology, PGIMER and Dr. RML Hospital, New Delhi 110001, India.
authors.forename: Preeti
authors.initials: P
authors.lastname: Madan
authors.affiliation: Department of Microbiology, PGIMER and Dr. RML Hospital, New Delhi 110001, India.
authors.forename: Wasim
authors.initials: W
authors.lastname: Ahmad
authors.affiliation: Department of Microbiology, PGIMER and Dr. RML Hospital, New Delhi 110001, India.
authors.forename: Charoo
authors.initials: C
authors.lastname: Hans