title: Response of bacterial community compositions to different sources of pollutants in sediments of a tributary of Taihu Lake, China.
abstract: Sediment bacterial communities are sensitive to water conditions in river ecosystems. The objective of this study was to compare the influences of different pollution sources, including urban areas, wastewater treatment plants (WWTPs), suburban areas, and agricultural areas, on sediment bacterial communities along a typical tributary of Taihu Lake, China. The dominant composition of the sediment bacterial community was determined using a combination of terminal restriction fragment length polymorphism (T-RFLP) and a 16S rRNA clone library. The results showed that the sediment bacterial communities were distinctly affected by the four pollution sources. Alphaproteobacteria, Betaproteobacteria, and Actinobacteria (>50 % in total) were the predominant bacterial taxa across the sediment samples. Apart from those, the sediment bacterial community composition (BCC) affected by WWTP effluent was subsequently dominated by Nitrospira (12.4 %) and Bacteroidetes (11.5 %), agriculture was dominated by Firmicutes (13.2 %) and Deltaproteobacteria (7.2 %), while urban and suburban were dominated by Bacteroidetes (7.6 and 7.9 %, respectively) and Deltaproteobacteria (7.9 and 7.6 %, respectively). Cluster analysis indicated that the BCC affected by WWTP effluent was distinct from the BCC in urban, suburban, and agricultural areas. In addition, the bacterial community richness and evenness affected by WWTP effluent were much less than those by the other pollution sources. Redundancy analysis (RDA) indicated that the variation in BCC across the sediment samples was significantly associated with ammonium (17 %), organic matter (12 %), and cadmium (3 %) (p < 0.01). Overall, the results indicated that the four different pollution sources (WWTP, urban, suburban, and agriculture) have dissimilar impacts on the sediment BCC in the tributary of Taihu Lake, while WWTPs exhibited the greatest potential to lead to biotic homogenization in river sediments.
authors.affiliation: Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Xikang Road #1, Nanjing, 210098, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Xikang Road #1, Nanjing, 210098, People's Republic of China. envly@hhu.edu.cn.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Xikang Road #1, Nanjing, 210098, People's Republic of China.
authors.forename: Peifang
authors.initials: P
authors.lastname: Wang
authors.affiliation: Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Xikang Road #1, Nanjing, 210098, People's Republic of China. Nlhnq55@163.com.
authors.forename: Lihua
authors.initials: L
authors.lastname: Niu
authors.affiliation: Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Xikang Road #1, Nanjing, 210098, People's Republic of China.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Xikang Road #1, Nanjing, 210098, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics*; D002681:China; D017753:Ecosystem; D019015:Geologic Sediments / Q000382:microbiology*; D060106:Lakes / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology*; D062065:Wastewater; D014871:Water Microbiology; D014874:Water Pollutants, Chemical
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Agriculture; Bacterial community; River ecosystem; Sediment; Suburban; Urban; Wastewater treatment plant