title: Lack of Associations between TNF-αPolymorphisms and Cervical Cancer in Thai women.
abstract: The risk of developing cervical cancer in women infected with human papillomavirus (HPV) may be influenced by an individual's genetic susceptibility. Published data linking single nucleotide polymorphisms (SNPs) in the tumor necrosis factor-alpha (TNF-?) promoter region at positions -308G>A (rs1800629) and -238G>A (rs361525) to cervical cancer risk have been inconclusive. In this study, we examined 251 cervical specimens and classified them into two groups according to their cytological findings: 121 cancer cases and 130 controls (low-grade squamous intraepithelial lesion and normal cytology). All specimens were typed by PCR and sequencing for TNF-αpromoter -308G>A (rs1800629) and -238G>A (rs361525). The genotype distribution of SNPs in either rs1800629 or rs361525 did not significantly demonstrate higher frequency in the cancer group (p=0.621 and p=0.68, respectively). Based on these results, neither the TNF-αpromoter -308G>A (rs1800629) nor the -238G>A (rs361525) polymorphism presents a major risk factor for cervical cancer among Thai women. Larger studies are necessary to elucidate possible genetic mechanisms influencing cervical cancer development.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand E-mail : yong.p@chula.ac.th.
authors.forename: Teeraporn
authors.initials: T
authors.lastname: Chinchai
authors.forename: Krittaphak
authors.initials: K
authors.lastname: Homchan
authors.forename: Watanyoo
authors.initials: W
authors.lastname: Sopipong
authors.forename: Jira
authors.initials: J
authors.lastname: Chansaenroj
authors.forename: Sukumarn
authors.initials: S
authors.lastname: Swangvaree
authors.forename: Pairoj
authors.initials: P
authors.lastname: Junyangdikul
authors.forename: Sompong
authors.initials: S
authors.lastname: Vongpunsawad
authors.forename: Yong
authors.initials: Y
authors.lastname: Poovorawan
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000472:pathogenicity; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012307:Risk Factors; D013785:Thailand; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000821:virology; D055815:Young Adult
chemical_list: D014409:Tumor Necrosis Factor-alpha