title: Use of a Chimeric Hsp70 to Enhance the Quality of Recombinant Plasmodium falciparum S-Adenosylmethionine Decarboxylase Protein Produced in Escherichia coli.
abstract: S-adenosylmethionine decarboxylase (PfAdoMetDC) from Plasmodium falciparum is a prospective antimalarial drug target. The production of recombinant PfAdoMetDC for biochemical validation as a drug target is important. The production of PfAdoMetDC in Escherichia coli has been reported to result in unsatisfactory yields and poor quality product. The co-expression of recombinant proteins with molecular chaperones has been proposed as one way to improve the production of the former in E. coli. E. coli heat shock proteins DnaK, GroEL-GroES and DnaJ have previously been used to enhance production of some recombinant proteins. However, the outcomes were inconsistent. An Hsp70 chimeric protein, KPf, which is made up of the ATPase domain of E. coli DnaK and the substrate binding domain of P. falciparum Hsp70 (PfHsp70) has been previously shown to exhibit chaperone function when it was expressed in E. coli cells whose resident Hsp70 (DnaK) function was impaired. We proposed that because of its domain constitution, KPf would most likely be recognised by E. coli Hsp70 co-chaperones. Furthermore, because it possesses a substrate binding domain of plasmodial origin, KPf would be primed to recognise recombinant PfAdoMetDC expressed in E. coli. First, using site-directed mutagenesis, followed by complementation assays, we established that KPf with a mutation in the hydrophobic residue located in its substrate binding cavity was functionally compromised. We further co-expressed PfAdoMetDC with KPf, PfHsp70 and DnaK in E. coli cells either in the absence or presence of over-expressed GroEL-GroES chaperonin. The folded and functional status of the produced PfAdoMetDC was assessed using limited proteolysis and enzyme assays. PfAdoMetDC co-expressed with KPf and PfHsp70 exhibited improved activity compared to protein co-expressed with over-expressed DnaK. Our findings suggest that chimeric KPf may be an ideal Hsp70 co-expression partner for the production of recombinant plasmodial proteins in E. coli.
authors.affiliation: Department of Biochemistry and Microbiology, University of Zululand, KwaDlangezwa, South Africa.
authors.forename: Xolani Henry
authors.initials: XH
authors.lastname: Makhoba
authors.affiliation: Department of Biochemistry, School of Mathematical & Natural Sciences, University of Venda, Thohoyandou, South Africa.
authors.forename: Adélle
authors.initials: A
authors.lastname: Burger
authors.affiliation: Department of Biochemistry, Centre for Sustainable Malaria Control, University of Pretoria, Pretoria, South Africa.
authors.forename: Dina
authors.initials: D
authors.lastname: Coertzen
authors.affiliation: Department of Biochemistry, School of Mathematical & Natural Sciences, University of Venda, Thohoyandou, South Africa.
authors.forename: Tawanda
authors.initials: T
authors.lastname: Zininga
authors.affiliation: Department of Biochemistry, Centre for Sustainable Malaria Control, University of Pretoria, Pretoria, South Africa.
authors.forename: Lyn-Marie
authors.initials: LM
authors.lastname: Birkholtz
authors.affiliation: Department of Biochemistry, School of Mathematical & Natural Sciences, University of Venda, Thohoyandou, South Africa.
authors.forename: Addmore
authors.initials: A
authors.lastname: Shonhai
mesh_terms: D012437:Adenosylmethionine Decarboxylase / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D050956:HSP40 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism; D010963:Plasmodium falciparum / Q000235:genetics*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D018832:Molecular Chaperones; D011994:Recombinant Proteins; C063842:dnaK protein, E coli; D012437:Adenosylmethionine Decarboxylase