title: Myosin heavy chain is stabilized by BCL-2 interacting cell death suppressor (BIS) in skeletal muscle.
abstract: BCL-2 interacting cell death suppressor (BIS), which is ubiquitously expressed, has important roles in various cellular processes, such as apoptosis, the cellular stress response, migration and invasion and protein quality control. In particular, BIS is highly expressed in skeletal and cardiac muscles, and BIS gene mutations result in human myopathy. In this study, we show that mRNA and protein levels of BIS were markedly increased during skeletal myogenesis in C2C12 cells and mouse satellite cells. BIS knockdown did not prevent the early stage of skeletal myogenesis, but did induce muscle atrophy and a decrease in the diameter of myotubes. BIS knockdown significantly suppressed the expression level of myosin heavy chain (MyHC) without changing the expression levels of myogenic marker proteins, such as Mgn, Cav-3 and MG53. In addition, BIS endogenously interacted with MyHC, and BIS knockdown induced MyHC ubiquitination and degradation. From these data, we conclude that molecular association of MyHC and BIS is necessary for MyHC stabilization in skeletal muscle.
authors.affiliation: Tunneling Nanotube Research Center, Korea University, Seoul, Republic of Korea.|Division of Life Sciences, Korea University, Seoul, Republic of Korea.
authors.forename: Jin
authors.initials: J
authors.lastname: Hong
authors.affiliation: Tunneling Nanotube Research Center, Korea University, Seoul, Republic of Korea.|Division of Life Sciences, Korea University, Seoul, Republic of Korea.
authors.forename: Jun-Sub
authors.initials: JS
authors.lastname: Park
authors.affiliation: Tunneling Nanotube Research Center, Korea University, Seoul, Republic of Korea.|Division of Life Sciences, Korea University, Seoul, Republic of Korea.
authors.forename: Hyun
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of Surgery, Hanyang University College of Medicine, Seoul, Republic of Korea.
authors.forename: Jaemin
authors.initials: J
authors.lastname: Jeong
authors.affiliation: Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.|Institute for Aging and Metabolic Diseases, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Hye
authors.initials: H
authors.lastname: Hyeon Yun
authors.affiliation: Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.|Institute for Aging and Metabolic Diseases, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Hye
authors.initials: H
authors.lastname: Yun Kim
authors.affiliation: Tunneling Nanotube Research Center, Korea University, Seoul, Republic of Korea.|Division of Life Sciences, Korea University, Seoul, Republic of Korea.
authors.forename: Young-Gyu
authors.initials: YG
authors.lastname: Ko
authors.affiliation: Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.|Institute for Aging and Metabolic Diseases, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Jeong-Hwa
authors.initials: JH
authors.lastname: Lee
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D015870:Gene Expression; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D024510:Muscle Development / Q000235:genetics; D018482:Muscle, Skeletal / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000378:metabolism*; D011485:Protein Binding; D055550:Protein Stability; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D032496:Satellite Cells, Skeletal Muscle / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; C414431:BAG3 protein, human; D012333:RNA, Messenger; D018995:Myosin Heavy Chains