title: Reproduction of post-weaning multi-systemic wasting syndrome in an animal disease model as a tool for vaccine testing under controlled conditions.
abstract: Snatch farrowed, colostrum deprived piglets were inoculated with different combinations of porcine circovirus 2, porcine parvovirus and Erysipelothrix rhusiopathiae candidate vaccines. 10 piglets were mock-vaccinated. Following virus challenge with a combined porcine circovirus 2/porcine parvovirus inoculum, all animals were monitored and samples taken for serology, immunohistochemistry and qPCR. At 24 dpc all non-vaccinated animals remaining were exhibiting signs of post-weaning multi-systemic wasting syndrome which was confirmed by laboratory analysis. Details of the study, analysis of samples and performance of the candidate vaccines are described.
authors.affiliation: Veterinary Sciences Division, Agri-Food and Biosciences Institute, Stormont, Belfast BT4 3SD, United Kingdom. Electronic address: john.mckillen@afbini.gov.uk.
authors.forename: John
authors.initials: J
authors.lastname: McKillen
authors.affiliation: Veterinary Sciences Division, Agri-Food and Biosciences Institute, Stormont, Belfast BT4 3SD, United Kingdom.
authors.forename: Irene
authors.initials: I
authors.lastname: McNair
authors.affiliation: Veterinary Sciences Division, Agri-Food and Biosciences Institute, Stormont, Belfast BT4 3SD, United Kingdom.
authors.forename: Paula
authors.initials: P
authors.lastname: Lagan
authors.affiliation: Veterinary Sciences Division, Agri-Food and Biosciences Institute, Stormont, Belfast BT4 3SD, United Kingdom.
authors.forename: Karen
authors.initials: K
authors.lastname: McKay
authors.affiliation: Veterinary Sciences Division, Agri-Food and Biosciences Institute, Stormont, Belfast BT4 3SD, United Kingdom.
authors.forename: Julie
authors.initials: J
authors.lastname: McClintock
authors.affiliation: Veterinary Sciences Division, Agri-Food and Biosciences Institute, Stormont, Belfast BT4 3SD, United Kingdom.
authors.forename: Veronica
authors.initials: V
authors.lastname: Casement
authors.affiliation: Merial SAS, 29 Avenue Tony Garnier, 69007 Lyon, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Charreyre
authors.affiliation: School of Biological Sciences, Medical Biology Centre, Queen's University of Belfast, 97 Lisburn Road, Belfast BT9 7BL, United Kingdom.
authors.forename: Gordon
authors.initials: G
authors.lastname: Allan
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000494:pharmacology; D017925:Circovirus / Q000276:immunology*; D004195:Disease Models, Animal; D004888:Erysipelothrix / Q000276:immunology; D004889:Erysipelothrix Infections / Q000276:immunology / Q000382:microbiology; D010322:Parvoviridae Infections / Q000276:immunology / Q000821:virology; D028323:Parvovirus, Porcine / Q000276:immunology; D053570:Porcine Postweaning Multisystemic Wasting Syndrome / Q000276:immunology* / Q000821:virology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000382:microbiology / Q000821:virology; D014765:Viral Vaccines / Q000494:pharmacology*
chemical_list: D001428:Bacterial Vaccines; D014765:Viral Vaccines
keywords: Animal model; Porcine circovirus; Porcine parvovirus; Quantitative PCR; Vaccine