title: LncRNA-UCA1 enhances cell proliferation and 5-fluorouracil resistance in colorectal cancer by inhibiting miR-204-5p.
abstract: Recent preliminary studies reported the in vitro tumor-promoting effects of long non-coding RNA urothelial carcinoma associated 1 (UCA1) in colorectal cancer (CRC). However, the in vivo functions and molecular mechanism of UCA1 in CRC remain unclear. Therefore, we investigated the detailed role and mechanism of UCA1 in CRC. We found that UCA1 was up-regulated in CRCs and negatively correlated with survival time in two CRC cohorts. Functional assays revealed the in vitro and in vivo growth-promoting function of UCA1 and revealed that UCA1 can decrease the sensitivity of CRC cells to 5-FU by attenuating apoptosis. Further mechanistic studies revealed that UCA1 could sponge endogenous miR-204-5p and inhibit its activity. We also identified CREB1 as a new target of miR-204-5p. The protein levels of CREB1 were significantly up-regulated in CRCs, negatively associated with survival time and positively correlated with the UCA1 expression. The present work provides the first evidence of a UCA1-miR-204-5p-CREB1/BCL2/RAB22A regulatory network in CRC and reveals that UCA1 and CREB1 are potential new oncogenes and prognostic factors for CRC.
authors.affiliation: Wuxi Oncology Institute, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, 214062, China.
authors.forename: Zehua
authors.initials: Z
authors.lastname: Bian
authors.affiliation: Department of Surgical Oncology, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, 214062, China.|Department of Surgery, the Second Affiliated Hospital of Soochow University, Suzhou, 215004, China.
authors.forename: Liugen
authors.initials: L
authors.lastname: Jin
authors.affiliation: Wuxi Oncology Institute, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, 214062, China.
authors.forename: Jiwei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Wuxi Oncology Institute, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, 214062, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Wuxi Oncology Institute, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, 214062, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Quan
authors.affiliation: Wuxi Oncology Institute, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, 214062, China.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Wuxi Oncology Institute, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, 214062, China.
authors.forename: Yuyang
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Wuxi Oncology Institute, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, 214062, China.
authors.forename: Heyong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Surgical Oncology, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, 214062, China.
authors.forename: Bojian
authors.initials: B
authors.lastname: Fei
authors.affiliation: Wuxi Oncology Institute, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, 214062, China.|Department of Oncology, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, 214062, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Wuxi Oncology Institute, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, 214062, China.|Department of Oncology, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, 214062, China.
authors.forename: Leyuan
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Pathology, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, 214062, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Qi
authors.affiliation: Institutes of Biomedical Sciences and Shanghai Cancer Center, Shanghai Medical College, Fudan University, Shanghai, 200032, China.
authors.forename: Shenlin
authors.initials: S
authors.lastname: Huang
authors.affiliation: Wuxi Oncology Institute, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, 214062, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Hua
authors.affiliation: Department of Surgery, the Second Affiliated Hospital of Soochow University, Suzhou, 215004, China.
authors.forename: Chungen
authors.initials: C
authors.lastname: Xing
authors.affiliation: Wuxi Oncology Institute, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, 214062, China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Huang
mesh_terms: D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000188:drug therapy / Q000235:genetics*; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics / Q000378:metabolism*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005472:Fluorouracil / Q000737:chemistry*; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D009857:Oncogenes; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000235:genetics*; D015533:Transcriptional Activation; D015854:Up-Regulation
chemical_list: C115163:CREB1 protein, human; C492004:Creb1 protein, mouse; D017362:Cyclic AMP Response Element-Binding Protein; C549404:MIRN204 microRNA, human; C574692:MIRN204 microRNA, mouse; D035683:MicroRNAs; D062085:RNA, Long Noncoding; D005472:Fluorouracil