title: Evidence for the involvement of nematocidal toxins of Purpureocillium lilacinum 6029 cultured on Karanja deoiled cake liquid medium.
abstract: In present study, in vitro nematocidal bioassays, FT-IR and HPLC analysis were employed to demonstrate the involvement of toxins of Purpureocillium lilacinum in killing root-knot nematodes (Meloidogyne incognita). During growth study, maximum mycelial biomass (10.52 g/l) in de-oiled Karanja cake medium was achieved on 8th day while complete mortality of nematodes was obtained by 6th day filtrate (FKSM). Maximum production of crude nematocidal toxin was recorded on 7th day suggesting that the toxin production was paralleled with growth of the fungus. The median lethal concentration (LC50) determined for the crude toxin from 6th day to 10th day ranged from 89.41 to 43.21 ppm. The median lethal time (LT50) for the crude toxin of FKSM was found to be 1.46 h. This is the first report of implementing a comparative infra-red spectroscopy coupled with HPLC analysis to predict the presence of nematocidal toxin in the fungal filtrate cultured on Karanja deoiled cake liquid medium.
authors.affiliation: Centre for Rural Development and Technology, Indian Institute of Technology Delhi, 289, Hauz Khas, New Delhi, 110016, India.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Centre for Rural Development and Technology, Indian Institute of Technology Delhi, 289, Hauz Khas, New Delhi, 110016, India. satyawatis@hotmail.com.
authors.forename: Satyawati
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Kusuma School of Biological Sciences, Indian Institute of Technology Delhi, New Delhi, India.
authors.forename: Aditya
authors.initials: A
authors.lastname: Mittal
authors.affiliation: Centre for Rural Development and Technology, Indian Institute of Technology Delhi, 289, Hauz Khas, New Delhi, 110016, India.
authors.forename: S N
authors.initials: SN
authors.lastname: Naik
mesh_terms: D000818:Animals; D000969:Antinematodal Agents / Q000378:metabolism* / Q000633:toxicity*; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*; D006999:Hypocreales / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D007928:Lethal Dose 50; D052596:Pongamia / Q000378:metabolism / Q000382:microbiology*; D014431:Tylenchoidea / Q000187:drug effects
chemical_list: D000969:Antinematodal Agents; D003470:Culture Media
keywords: HPLC; Karanja deoiled cake; Meloidogyne incognita; Purpureocillium lilacinum; Toxin