title: Genome-Wide Identification and Expression Analysis of the 14-3-3 Family Genes in Medicago truncatula.
abstract: The 14-3-3 gene family, which is conserved in eukaryotes, is involved in protein-protein interactions and mediates signal transduction. However, detailed investigations of the 14-3-3 gene family in Medicago truncatula are largely unknown. In this study, the identification and study of M. truncatula 14-3-3-family genes were performed based on the latest M. truncatula genome. In the M. truncatula genome, 10 14-3-3 family genes were identified, and they can be grouped into ε and non-ε groups. An exon-intron analysis showed that the gene structures are conserved in the same group. The protein structure analysis showed that 14-3-3 proteins in M. truncatula are composed of nine typical antiparallel α-helices. The expression patterns of Mt14-3-3 genes indicated that they are expressed in all tissues. Furthermore, the gene expression levels of Mt14-3-3 under hormone treatment and Sinorhizobium meliloti infection showed that the Mt14-3-3 genes were involve in nodule formation. Our findings lay a solid foundation for further functional studies of 14-3-3 in M. truncatula.
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University Hangzhou, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Qin
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University Hangzhou, China.
authors.forename: Linming
authors.initials: L
authors.lastname: Cheng
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University Hangzhou, China.
authors.forename: Jingqin
authors.initials: J
authors.lastname: Shen
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University Hangzhou, China.
authors.forename: Yunhong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University Hangzhou, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Cao
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University Hangzhou, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Lu
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University Hangzhou, China.
authors.forename: Chenjia
authors.initials: C
authors.lastname: Shen
keywords: 14-3-3 gene family; Medicago truncatula; Sinorhizobium meliloti infection; flowering; nodule formation