title: (-)-Epigallocatechin Gallate Inhibits Asymmetric Dimethylarginine-Induced Injury in Human Brain Microvascular Endothelial Cells.
abstract: (-)-Epigallocatechin gallate (EGCG) is the main polyphenol component of green tea (leaves of the Camellia sinensis plant). EGCG has been reported to protect human brain microvascular endothelial cells (HBMECs) against injury in several models. However, the exact mechanism is still unclear. In the current study we found that EGCG protected against asymmetric dimethylarginine (ADMA)-induced HBMEC injury, and inhibited ADMA-induced reactive oxygen species production and malondialdehyde expression. At the same time, we found that pretreatment with EGCG attenuated the upregulation of Bax and the downregulation of Bcl-2, thus confirming the cellular protective properties of EGCG against ADMA-induced apoptosis. Furthermore, we found that EGCG inhibited ADMA-induced phosphorylation of ERK1/2 and p-38, whose inhibitors relieved HBMEC injury. In conclusion, EGCG can protect against ADMA-induced HBMEC injury via the ERK1/2 and p38 MAPK pathways, which are involved in the underlying mechanisms of HBMEC injury in cerebral infarction.
authors.affiliation: Department of Neurosurgery, Tianjin Nankai Hospital, Tianjin, 300100, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Tianjin Nankai Hospital, Tianjin, 300100, China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Tianjin Nankai Hospital, Tianjin, 300100, China.
authors.forename: Lianjie
authors.initials: L
authors.lastname: Lv
authors.affiliation: Department of Neurosurgery, Tianjin Nankai Hospital, Tianjin, 300100, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Qiao
authors.affiliation: Department of Neurosurgery, Tianjin Nankai Hospital, Tianjin, 300100, China.
authors.forename: Xiuju
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, Tianjin Nankai Hospital, Tianjin, 300100, China. changlinzhoutj@sina.com.|Surgical Department, Tianjin Nankai Hospital, Tianjin, 300100, China. changlinzhoutj@sina.com.
authors.forename: Changlin
authors.initials: C
authors.lastname: Zou
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D001120:Arginine / Q000031:analogs & derivatives* / Q000633:toxicity; D001921:Brain / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D055806:Microvessels / Q000187:drug effects* / Q000293:injuries / Q000378:metabolism
chemical_list: D000975:Antioxidants; C018524:N,N-dimethylarginine; D002392:Catechin; D001120:Arginine; C045651:epigallocatechin gallate
keywords: (−)-Epigallocatechin gallate; Asymmetric dimethylarginine; Cerebral infarction; Human brain microvascular endothelial cells; Mitogen-activated protein kinases