title: Magnetic bead-based nucleic acid purification kit: Clinical application and performance evaluation in stool specimens.
abstract: Two different methods - the semi-automated magnetic bead-based kit (SK, Stool DNA/RNA Purification kit®) and the manual membrane column-based kit (QS, QIAamp® DNA Stool Mini kit) - for purifying nucleic acids from clinical stool samples were compared and evaluated. The SK kit was more user-friendly than QS due to the reduced manual processing, partial automation, and short turnaround time with half cost. Furthermore, SK produced high yields in both DNA and RNA extractions but poor purity in RNA extraction. In the assessment of rotavirus and Clostridium difficile infection, both kits had equivalent or more sensitive performance compared with the standard method. Although SK showed some interference and inhibition in nucleic acid extraction, the performance, including the repeatability, linearity, analytical sensitivity, and matrix effect, was sufficient for routine clinical use.
authors.affiliation: Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Republic of Korea.
authors.forename: Jihoon G
authors.initials: JG
authors.lastname: Yoon
authors.affiliation: Molecular Diagnostics Team, SK Telecom, Sungnam-si, Gyeonggi-do, Republic of Korea; Department of Molecular and Life Science, Hanyang University, Ansan, Gyeonggi-do, Republic of Korea.
authors.forename: Jin Seok
authors.initials: JS
authors.lastname: Kang
authors.affiliation: Department of Bio-Nanotechnology & Department of Molecular and Life Science, Hanyang University, Ansan, Gyeonggi-do, Republic of Korea.
authors.forename: Seung Yong
authors.initials: SY
authors.lastname: Hwang
authors.affiliation: Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Republic of Korea.
authors.forename: Jaewoo
authors.initials: J
authors.lastname: Song
authors.affiliation: Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Republic of Korea. Electronic address: kscpjsh@yuhs.ac.
authors.forename: Seok Hoon
authors.initials: SH
authors.lastname: Jeong
mesh_terms: D001331:Automation; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D008280:Magnetics / Q000295:instrumentation / Q000379:methods*; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic
chemical_list: D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic
keywords: Clostridium difficile; Nucleic acid extraction; Rotavirus; Stool