title: Infection of J774A.1 with different Mycobacterium species induces differential immune and miRNA-related responses.
abstract: Macrophages act as a reservoir for Mycobacterium tuberculosis, producing latent infection in approximately 90% of infected people. In this study, J774A.1 mouse macrophage cell line response and microRNA (miRNA) expression during infection with the most relevant mycobacterial strains for humans (M. tuberculosis, M. bovis and M. bovis BCG) was explored. No significant differences in bacillary loads were observed between activate and naive macrophages infected with M. tuberculosis and M. bovis. Nitrite production inhibition and infection control were in accordance with the virulence of the strain. Expression of let-7e, miR-21, miR-155, miR-210 and miR-223 was opposite in the two species and miR-146b* and miR-1224 expression seemed to be part of the general response to infection.
authors.affiliation: Genomic Sciences Program, Autonomous University of Mexico City, 290 San Lorenzo, Benito Juárez, Col del Valle Sur, 03100 Ciudad de México, México.
authors.forename: Mendoza-Coronel
authors.initials: MC
authors.lastname: Elizabeth
authors.affiliation: Genomic Sciences Program, Autonomous University of Mexico City, 290 San Lorenzo, Benito Juárez, Col del Valle Sur, 03100 Ciudad de México, México.
authors.forename: Olga Nohemí
authors.initials: ON
authors.lastname: Hernández de la Cruz
authors.affiliation: Genomic Sciences Program, Autonomous University of Mexico City, 290 San Lorenzo, Benito Juárez, Col del Valle Sur, 03100 Ciudad de México, México.
authors.forename: Castañón-Arreola
authors.initials: CA
authors.lastname: Mauricio
mesh_terms: D000818:Animals; D002460:Cell Line; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D007113:Immunity, Innate; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D009163:Mycobacterium bovis / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D009573:Nitrites / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D009573:Nitrites
keywords: macrophages; microRNA; mycobacterium