title: Cadaveric cardiosphere-derived cells can maintain regenerative capacity and improve the heart function of cardiomyopathy.
abstract: Cardiosphere-derived cells (CDCs) improve cardiac function and attenuate remodeling in ischemic and non-ischemic cardiomyopathy, and are currently obtained through myocardial biopsy. However, there is not any study on whether functional CDCs may be obtained through cadaveric autopsy with similar benefits in non-ischemic cardiomyopathy.
  
  
  
  Cardiac tissues from human or mouse cadavers were harvested, plated at 4°C, and removed at varying time points to culture human CDCs (CLH-EDCs) and mouse CDCs (CM-CDCs). The differentiation and paracrine effects of CDCs were also assessed. Furthermore, intramyocardial injection of cadaveric CM-CDCs was performed in an induced dilated cardiomyopathy (DCM) model.
  
  
  
  With the extension of post mortem hours, the number of CLH-EDCs and CM-CDCs harvested from autopsy specimens decreased. The expressions of von Willebrand factor (VWF) and smooth muscle actin (SMA) on CDCs were gradually reduced, however, cardiac troponin I (TNI) expression increased in the 24 h group compared to the 0 h group. CLH-EDCs were also found to have similar paracrine function in the 24 h group compared to 0 h group. 8 weeks after CM-CDCs transplantion to the injured heart, mean left ventricular ejection fraction increased in both 0 h (64.99 ± 3.4%) and 24 h (62.99 ± 2.8%) CM-CDCs-treated groups as compared to the PBS treated group (53.64 ± 5.6 cm), with a decrease in left ventricular internal diastolic diameter (0.29 ± 0.08 cm and 0.32 ± 0.04 cm in 0 h and 24 h groups, vs. 0.41 ± 0.05 cm in PBS group).
  
  
  
  CDCs from cadaveric autopsy are highly proliferative and differentiative, and may be used as a source for allograft transplantation, in order to decrease myocardial fibrosis, attenuate left ventricular remodeling, and improve heart function in doxorubicin-induced non-ischemic cardiomyopathy.
authors.affiliation: a Key Laboratories of Education Ministry for Myocardial Ischemia Mechanism and Treatment , Harbin Medical University , Harbin , China.|b Department of Cardiology , The Second Affiliated Hospital of Harbin Medical University , Harbin , China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Sun
authors.affiliation: a Key Laboratories of Education Ministry for Myocardial Ischemia Mechanism and Treatment , Harbin Medical University , Harbin , China.|b Department of Cardiology , The Second Affiliated Hospital of Harbin Medical University , Harbin , China.
authors.forename: Di
authors.initials: D
authors.lastname: Chi
authors.affiliation: c Department of Cardiology , The First Hospital of Fangshan District , Beijing , china.
authors.forename: Miaoxin
authors.initials: M
authors.lastname: Tan
authors.affiliation: d Department of Cardial Surgery , The Second Affiliated Hospital of Harbin Medical University , Harbin , China.
authors.forename: Kai
authors.initials: K
authors.lastname: Kang
authors.affiliation: a Key Laboratories of Education Ministry for Myocardial Ischemia Mechanism and Treatment , Harbin Medical University , Harbin , China.|b Department of Cardiology , The Second Affiliated Hospital of Harbin Medical University , Harbin , China.
authors.forename: Maomao
authors.initials: M
authors.lastname: Zhang
authors.affiliation: e Department of Thoracic Surgery , The Third Affiliated Hospital of Harbin Medical University , Harbin , China.
authors.forename: Xiangyuan
authors.initials: X
authors.lastname: Jin
authors.affiliation: f Department of Doppler Ultrasonic , The Second Affiliated Hospital of Harbin Medical University , Harbin , China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Leng
authors.affiliation: a Key Laboratories of Education Ministry for Myocardial Ischemia Mechanism and Treatment , Harbin Medical University , Harbin , China.|b Department of Cardiology , The Second Affiliated Hospital of Harbin Medical University , Harbin , China.
authors.forename: Rui
authors.initials: R
authors.lastname: Cao
authors.affiliation: b Department of Cardiology , The Second Affiliated Hospital of Harbin Medical University , Harbin , China.
authors.forename: Xianglan
authors.initials: X
authors.lastname: Liu
authors.affiliation: a Key Laboratories of Education Ministry for Myocardial Ischemia Mechanism and Treatment , Harbin Medical University , Harbin , China.|b Department of Cardiology , The Second Affiliated Hospital of Harbin Medical University , Harbin , China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
authors.affiliation: a Key Laboratories of Education Ministry for Myocardial Ischemia Mechanism and Treatment , Harbin Medical University , Harbin , China.|b Department of Cardiology , The Second Affiliated Hospital of Harbin Medical University , Harbin , China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D002102:Cadaver; D009202:Cardiomyopathies / Q000503:physiopathology*; D002454:Cell Differentiation; D002470:Cell Survival; D002648:Child; D002675:Child, Preschool; D050991:GATA4 Transcription Factor / Q000378:metabolism; D006321:Heart / Q000503:physiopathology*; D000072576:Homeobox Protein Nkx-2.5 / Q000378:metabolism; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D032383:Myocytes, Cardiac / Q000166:cytology*; D012038:Regeneration; D018874:Spheroids, Cellular / Q000166:cytology*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D013997:Time Factors; D055815:Young Adult
chemical_list: D050991:GATA4 Transcription Factor; D000072576:Homeobox Protein Nkx-2.5; D036341:Intercellular Signaling Peptides and Proteins
keywords: allograft transplantation; cadaver; cardiosphere-derived cells; dilated cardiomyopathy