title: Engineering a filamentous fungus for L-rhamnose extraction.
abstract: L-Rhamnose is a high value rare sugar that is used as such or after chemical conversions. It is enriched in several biomass fractions such as the pectic polysaccharides rhamnogalacturonan I and II and in naringin, hesperidin, rutin, quercitrin and ulvan. We engineered the filamentous fungus Aspergillus niger to not consume L-rhamnose, while it is still able to produce the enzymes for the hydrolysis of L-rhamnose rich biomass. As a result we present a strain that can be used for the extraction of L-rhamnose in a consolidated process. In the process the biomass is hydrolysed to the monomeric sugars which are consumed by the fungus leaving the L-rhamnose.
authors.affiliation: VTT Technical Research Centre of Finland Ltd., PO Box 1000, 02044 VTT, Espoo, Finland. joosu.kuivanen@vtt.fi.
authors.forename: Joosu
authors.initials: J
authors.lastname: Kuivanen
authors.affiliation: VTT Technical Research Centre of Finland Ltd., PO Box 1000, 02044 VTT, Espoo, Finland.
authors.forename: Peter
authors.initials: P
authors.lastname: Richard
keywords: Aspergillus niger; Citrus peel; Consolidated bioprocess; L-rhamnose; Metabolic engineering; Pectin; Rhamogalacturonan