title: C. elegans Methods to Study PTEN.
abstract: C. elegans encodes a PTEN homolog called DAF-18 and human PTEN can functionally replace DAF-18. Thus C. elegans provides a valuable model organism to study PTEN. This chapter provides methods to study DAF-18/PTEN function in C. elegans. We provide methods to genotype daf-18/Pten mutants, visualize and quantify DAF-18/PTEN in C. elegans, as well as to study physiological and developmental processes that will provide molecular insight on DAF-18/PTEN function.
authors.affiliation: Department of Biology, Queen's University, 116 Barrie St., Kingston, ON, Canada, K7L 3N6.
authors.forename: Shanqing
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Department of Biology, Queen's University, 116 Barrie St., Kingston, ON, Canada, K7L 3N6. chinsang@queensu.ca.
authors.forename: Ian D
authors.initials: ID
authors.lastname: Chin-Sang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D017173:Caenorhabditis elegans / Q000737:chemistry / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000032:analysis / Q000378:metabolism*; D008136:Longevity; D023421:Models, Animal
chemical_list: D029742:Caenorhabditis elegans Proteins; C116661:DAF-18 protein, C elegans
keywords: C. elegans; DAF-18; Molecular biology; PTEN; Tumor suppressor