title: Maintenance of Skin Epithelial Stem Cells by Wnt-3a In Vitro.
abstract: CD49f+ CD34+ cells, a population rich in skin epithelial stem cells (EpSCs), were obtained from adult mouse skin and cultured with Wnt-3a for 10 days. On day 10, CD49f+ CD34+ cells were sorted and subjected to a second 10-day culture with Wnt-3a. The same procedures were repeated until fifteenth 10-day culture. CD49f+ CD34+ cells obtained from each 10-day culture retained the same EpSC-characteristics as seen in the original EpSCs from adult mouse skin. Here, wedescribe the culture protocol using Wnt-3a for successful maintenance of EpSCs.
authors.affiliation: Department of Pathogen, Infection and Immunity, Nara Medical University, 840 Shijo-Cho, Kashihara, Nara, 634-8521, Japan. oujix@naramed-u.ac.jp.
authors.forename: Yukiteru
authors.initials: Y
authors.lastname: Ouji
authors.affiliation: Department of Pathogen, Infection and Immunity, Nara Medical University, 840 Shijo-Cho, Kashihara, Nara, 634-8521, Japan. myoshika@naramed-u.ac.jp.
authors.forename: Masahide
authors.initials: M
authors.lastname: Yoshikawa
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology; D051379:Mice; D012867:Skin / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D060508:Wnt3 Protein / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D060508:Wnt3 Protein
keywords: Cell proliferation; Epithelial cells; Skin; Stem cells; Wnt signaling; Wnt-3a