title: NOB1 silencing inhibits the growth and metastasis of laryngeal cancer cells through the regulation of JNK signaling pathway.
abstract: Nin one binding protein (NOB1) plays important roles in the synthesis and degradation of proteins, thus having effects on the cellular process. In the present study, the expression level of NOB1 in laryngeal cancer patients was detected by quantitative PCR and western blotting, and the effect of NOB1 on growth and metastasis of laryngeal cancer cells was explored. Silence of NOB1 was found to inhibit the proliferation of laryngeal cancer cells, arrest cell cycle and induce cell apoptosis. NOB1 silence was also found to inhibit the migration and invasion of laryngeal cancer cells and to downregulate the protein levels of matrix metalloproteinases (MMPs)-2 and MMP-9. Further mechanism study revealed that the JNK signaling pathway was involved in the function of NOB1. Our present results suggest that NOB1 plays an oncogenic role in laryngeal cancer cells through the regulation of JNK signaling pathway, and lays a theoretical foundation for further exploration of NOB1.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Shengjing Hospital of China Medical University, Heping, Shenyang, Liaoning 110004, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Shengjing Hospital of China Medical University, Heping, Shenyang, Liaoning 110004, P.R. China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Shengjing Hospital of China Medical University, Heping, Shenyang, Liaoning 110004, P.R. China.
authors.forename: Weiliang
authors.initials: W
authors.lastname: Bai
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Shengjing Hospital of China Medical University, Heping, Shenyang, Liaoning 110004, P.R. China.
authors.forename: Wenyue
authors.initials: W
authors.lastname: Ji
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Shengjing Hospital of China Medical University, Heping, Shenyang, Liaoning 110004, P.R. China.
authors.forename: Liping
authors.initials: L
authors.lastname: Wang
mesh_terms: D017209:Apoptosis; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000502:physiology*; D007822:Laryngeal Neoplasms / Q000473:pathology*; D020935:MAP Kinase Signaling System / Q000502:physiology*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009687:Nuclear Proteins / Q000032:analysis / Q000037:antagonists & inhibitors / Q000502:physiology*; D016601:RNA-Binding Proteins / Q000032:analysis / Q000037:antagonists & inhibitors / Q000502:physiology*
chemical_list: C506664:NOB1 protein, human; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D048031:JNK Mitogen-Activated Protein Kinases