title: Determination of ochratoxin A in pig tissues using enzymatic digestion coupled with high-performance liquid chromatography with a fluorescence detector.
abstract: We present a new method for the rapid analysis of ochratoxin A (OTA) in pig tissues (muscle, liver and kidney) using enzymatic digestion (ED) coupled to high-performance liquid chromatography with a fluorescence detector (HPLC-FLD). OTA was digested with a 1% pancreatin solution in a phosphate buffer and then cleaned with ethylacetate. After being evaporated to dryness and re-dissolved, the sample was determined using HPLC-FLD. The method was validated taking into account the currently permitted limit of 1 μg/kg OTA in pork meat and derived products in Italy. The recovery was higher than 90%. Intra- and inter-day repeatability expressed as RSD were less than 7%. The LOD and LOQ were 0.001 and 0.002 μg/kg, respectively. Our method is more efficient, easier, and cheaper than conventional clean-up procedures (liquid-liquid extraction). •The aim of the study was to develop and validate a quantitative HPLC-FLD method based on ED followed by a chromatographic analysis without any previous clean-up or concentration step for the detection of OTA in pig tissues.•The ED method showed a 90%+ recovery, and intra- and inter-day RSD less than 7%.•This method is simple, rapid, easy to use, and consumes low amounts of organic solvents.
authors.affiliation: Department of Veterinary Science, University of Pisa, Italy.
authors.forename: Luci
authors.initials: L
authors.lastname: Giacomo
authors.affiliation: Department of Veterinary Science, University of Pisa, Italy.
authors.forename: Vanni
authors.initials: V
authors.lastname: Michele
authors.affiliation: Department of Veterinary Science, University of Pisa, Italy.
authors.forename: Ferruzzi
authors.initials: F
authors.lastname: Guido
authors.affiliation: Department of Veterinary Science, University of Pisa, Italy.
authors.forename: Mani
authors.initials: M
authors.lastname: Danilo
authors.affiliation: Department of Veterinary Science, University of Pisa, Italy.
authors.forename: Intorre
authors.initials: I
authors.lastname: Luigi
authors.affiliation: Department of Veterinary Science, University of Pisa, Italy.
authors.forename: Meucci
authors.initials: M
authors.lastname: Valentina
keywords: Enzyme assisted digestion; Enzyme assisted digestion coupled to HPLC-FLD; Kidney; Liver; Muscle; Ochratoxin A; Pig