title: Lipase-catalyzed in-situ biosynthesis of glycerol-free biodiesel from heterotrophic microalgae, Aurantiochytrium sp. KRS101 biomass.
abstract: Heterotrophic microalgae, Aurantiochytrium sp. KRS101 had a large amount of lipid (56.8% total lipids). The cells in the culture medium were easily ruptured due to thin cell wall of Aurantiochytrium sp., which facilitated in-situ fatty acid methyl esters (FAMEs) production directly from biomass. The harvested biomass had a high content of free fatty acids (FFAs), which was advantageous for glycerol-free FAMEs production. FAMEs were directly produced from Aurantiochytrium sp. KRS101 biomass (48.4% saponifiable lipids) using Novozyme 435-catalyzed in-situ esterification in dimethyl carbonate (DMC). DMC was used as a lipid extraction reagent, acyl acceptor and reaction medium. A 433.09mg FAMEs/g biomass was obtained with 89.5% conversion under the optimal condition: DMC to biomass ratio of 5:1 (v/w) and enzyme to biomass ratio of 30% (w/w) at 50°C for 12h. Glycerol could not be detected in the produced FAMEs.
authors.affiliation: Department of Chemical Engineering, Kyung Hee University, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: Keon Hee
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Department of Chemical Engineering, Kyung Hee University, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: Ok Kyung
authors.initials: OK
authors.lastname: Lee
authors.affiliation: Microbe-based Fusion Technology Research Center and Industrial Microbiology & Bioprocess Research Center, Jeonbuk Branch Institute, Korean Research Institute of Bioscience and Biotechnology, Jeonbuk 580-185, Republic of Korea.
authors.forename: Chul Ho
authors.initials: CH
authors.lastname: Kim
authors.affiliation: Microbe-based Fusion Technology Research Center and Industrial Microbiology & Bioprocess Research Center, Jeonbuk Branch Institute, Korean Research Institute of Bioscience and Biotechnology, Jeonbuk 580-185, Republic of Korea.
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Seo
authors.affiliation: Microbe-based Fusion Technology Research Center and Industrial Microbiology & Bioprocess Research Center, Jeonbuk Branch Institute, Korean Research Institute of Bioscience and Biotechnology, Jeonbuk 580-185, Republic of Korea.
authors.forename: Baek-Rock
authors.initials: BR
authors.lastname: Oh
authors.affiliation: Department of Chemical Engineering, Kyung Hee University, Gyeonggi-do 446-701, Republic of Korea. Electronic address: eunylee@khu.ac.kr.
authors.forename: Eun Yeol
authors.initials: EY
authors.lastname: Lee
mesh_terms: D055162:Biocatalysis / Q000187:drug effects; D056804:Biofuels; D018533:Biomass; D004800:Enzymes, Immobilized; D004951:Esterification / Q000187:drug effects; D004952:Esters / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005561:Formates / Q000494:pharmacology; D005656:Fungal Proteins; D005990:Glycerol / Q000378:metabolism*; D052836:Heterotrophic Processes / Q000187:drug effects; D006878:Hydroxides / Q000494:pharmacology; D008049:Lipase / Q000378:metabolism*; D058086:Microalgae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D017680:Potassium Compounds / Q000494:pharmacology; D012997:Solvents; D058009:Stramenopiles / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000494:pharmacology
chemical_list: D056804:Biofuels; D004800:Enzymes, Immobilized; D004952:Esters; D005230:Fatty Acids, Nonesterified; D005561:Formates; D005656:Fungal Proteins; D006878:Hydroxides; D017680:Potassium Compounds; D012997:Solvents; D014867:Water; C093667:Novozyme 435; D008049:Lipase; C023025:methyl carbonate; D005990:Glycerol; C029943:potassium hydroxide
keywords: Aurantiochytrium sp. KRS101; Biodiesel; Fatty acid methyl esters; Heterotrophic microalgae; In-situ esterification