title: Induction of Functional Hair-Cell-Like Cells from Mouse Cochlear Multipotent Cells.
abstract: In this paper, we developed a two-step-induction method of generating functional hair cells from inner ear multipotent cells. Multipotent cells from the inner ear were established and induced initially into progenitor cells committed to the inner ear cell lineage on the poly-L-lysine substratum. Subsequently, the committed progenitor cells were cultured on the mitotically inactivated chicken utricle stromal cells and induced into hair-cell-like cells containing characteristic stereocilia bundles. The hair-cell-like cells exhibited rapid permeation of FM1-43FX. The whole-cell patch-clamp technique was used to measure the membrane currents of cells differentiated for 7 days on chicken utricle stromal cells and analyze the biophysical properties of the hair-cell-like cells by recording membrane properties of cells. The results suggested that the hair-cell-like cells derived from inner ear multipotent cells were functional following differentiation in an enabling environment.
authors.affiliation: Institute of Cell and Development, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Quanwen
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Epidemiology & Health Statistics, School of Medicine, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Institute of Cell and Development, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jiarong
authors.initials: J
authors.lastname: Chen
authors.affiliation: Institute of Cell and Development, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Ding
authors.affiliation: Institute of Cell and Development, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Zihua
authors.initials: Z
authors.lastname: Tang
authors.affiliation: Institute of Cell and Development, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Cui
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Institute of Cell and Development, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jianling
authors.initials: J
authors.lastname: Chen
authors.affiliation: Institute of Cell and Development, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.affiliation: Institute of Cell and Development, College of Life Sciences, Zhejiang University, Hangzhou 310058, China; Departments of Cell Biology and Otolaryngology, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.affiliation: Institute of Cell and Development, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jinfu
authors.initials: J
authors.lastname: Wang