title: Molecular cloning, recombinant expression, and antifungal functional characterization of the lipid transfer protein from Panax ginseng.
abstract: OBJECTIVE
  To establish an efficient expression system for a fusion protein GST-pgLTP (Lipid Transfer Protein) and to test its antifungal activity.
  
  
  RESULTS
  The nucleotide sequence of LTP gene was obtained from Panax ginseng using RT-PCR. The ORF of the cDNA is 363 bp, codING for a protein OF 120 amino acids with a calculated MW of 12.09 kDa. The pgLTP gene with a His6-tag at the C-terminus was cloned into the pGEX-6p1 vector to generate a GST-fusion pgLTP protein construct that was expressed in Escherichia coli Rosetta. Following purification by Ni-NTA, the fusion protein exhibited antifungal activity against five fungi found in ginseng.
  
  
  CONCLUSIONS
  The fusion protein GST-pgLTP has activity against a broad spectrum of phytopathogenic fungi, and can potentially be adapted for production to combat fungal diseases that affect P. ginseng.
authors.affiliation: Traditional Chinese Medicine and Biotechnology Research and Development Center, Changchun University of Traditional Chinese Medicine, 1035 Boshuo Road, Changchun, Jilin, People's Republic of China.
authors.forename: Kexin
authors.initials: K
authors.lastname: Cai
authors.affiliation: Traditional Chinese Medicine and Biotechnology Research and Development Center, Changchun University of Traditional Chinese Medicine, 1035 Boshuo Road, Changchun, Jilin, People's Republic of China.
authors.forename: Jiawen
authors.initials: J
authors.lastname: Wang
authors.affiliation: Traditional Chinese Medicine and Biotechnology Research and Development Center, Changchun University of Traditional Chinese Medicine, 1035 Boshuo Road, Changchun, Jilin, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Traditional Chinese Medicine and Biotechnology Research and Development Center, Changchun University of Traditional Chinese Medicine, 1035 Boshuo Road, Changchun, Jilin, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Traditional Chinese Medicine and Biotechnology Research and Development Center, Changchun University of Traditional Chinese Medicine, 1035 Boshuo Road, Changchun, Jilin, People's Republic of China.
authors.forename: Siming
authors.initials: S
authors.lastname: Wang
authors.affiliation: Traditional Chinese Medicine and Biotechnology Research and Development Center, Changchun University of Traditional Chinese Medicine, 1035 Boshuo Road, Changchun, Jilin, People's Republic of China. cnzhaoyu1972@126.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000737:chemistry / Q000145:classification / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D005894:Panax / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*
chemical_list: D000935:Antifungal Agents; D002352:Carrier Proteins; D018076:DNA, Complementary; D010940:Plant Proteins; D011994:Recombinant Proteins; C030363:lipid transfer protein
keywords: Antifungal activity; Lipid transfer proteins; Panax ginseng; Protein expression and purification