title: Gene expression profiles of putative biomarker candidates in Mycobacterium avium subsp. paratuberculosis-infected cattle.
abstract: This study was conducted to analyze the gene expression of prognostic potential biomarker candidates using the whole blood of cattle naturally infected with ITALIC! Mycobacterium aviumsubsp. ITALIC! paratuberculosis(MAP). We conducted real-time PCR to evaluate 23 potential biomarker candidates. Experimental animals were divided into four groups based on fecal MAP PCR and serum ELISA. Seven ( ITALIC! KLRB1, ITALIC! HGF, ITALIC! MPO, ITALIC! LTF, ITALIC! SERPINE1, ITALIC! S100A8and ITALIC! S100A9) genes were up-regulated in fecal MAP-positive cattle and three ( ITALIC! KLRB1, ITALIC! MPOand ITALIC! S100A9) were up-regulated in MAP-seropositive cattle relative to uninfected cattle. In subclinically infected animals, 17 genes ( ITALIC! TFRC, ITALIC! S100A8, ITALIC! S100A9, ITALIC! MPO, ITALIC! GBP6, ITALIC! LTF, ITALIC! KLRB1, ITALIC! SERPINE1, ITALIC! PIGR, ITALIC! IL-10, ITALIC! CXCR3, ITALIC! CD14, ITALIC! MMP9, ITALIC! ELANE, ITALIC! CHI3L1, ITALIC! HPand ITALIC! HGF) were up-regulated compared with the control group. Moreover, six genes ( ITALIC! CXCR3, ITALIC! HP, ITALIC! HGF, ITALIC! LTF, ITALIC! TFRCand ITALIC! GBP6) showed significant differences between experimental groups. Taken together, our data suggest that six genes ( ITALIC! LTF, ITALIC! HGF, ITALIC! HP, ITALIC! CXCR3, ITALIC! GBP6and ITALIC! TFRC) played essential roles in the immune response to MAP during the subclinical stage and therefore might be useful as prognostic biomarkers.
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Hyun-Eui
authors.initials: HE
authors.lastname: Park
authors.affiliation: Department of Microbiology, College of Medicine, Institute of Health Sciences, Gyeongsang National University, Jinju 52727, Republic of Korea.
authors.forename: Min-Kyoung
authors.initials: MK
authors.lastname: Shin
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Hong-Tae
authors.initials: HT
authors.lastname: Park
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Myunghwan
authors.initials: M
authors.lastname: Jung
authors.affiliation: Department of Animal Resources Development, National Institute of Animal Science, Rural Development Administration, Cheonan 31000, Republic of Korea.
authors.forename: Yong Il
authors.initials: YI
authors.lastname: Cho
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul 08826, Republic of Korea Institute of Green Bio Science and Technology, Seoul National University, Pyeongchang 25354, Republic of Korea yoohs@snu.ac.kr.
authors.forename: Han Sang
authors.initials: HS
authors.lastname: Yoo
mesh_terms: D000818:Animals; D015415:Biomarkers; D002417:Cattle; D020869:Gene Expression Profiling; D016927:Mycobacterium avium subsp. paratuberculosis; D010283:Paratuberculosis / Q000235:genetics* / Q000382:microbiology*; D059467:Transcriptome
chemical_list: D015415:Biomarkers
keywords: Biomarkers; Cattle; Paratuberculosis; Subclinical stage