title: An overview on the delivery of antitumor drug doxorubicin by carrier proteins.
abstract: Serum proteins play an increasing role as drug carriers in the clinical settings. In this review, we have compared the binding modalities of anticancer drug doxorubicin (DOX) to three model carrier proteins, human serum albumin (HSA), bovine serum albumin (BSA) and milk beta-lactoglobulin (β-LG) in order to determine the potential application of these model proteins in DOX delivery. Molecular modeling studies showed stronger binding of DOX with HSA than BSA and β-LG with the free binding energies of -10.75 (DOX-HSA), -9.31 (DOX-BSA) and -8.12kcal/mol (DOX-β-LG). Extensive H-boding network stabilizes DOX-protein conjugation and played a major role in drug-protein complex formation. DOX complexation induced major alterations of HSA and BSA conformations, while did not alter β-LG secondary structure. The literature review shows that these proteins can potentially be used for delivery of DOX in vitro and in vivo.
authors.affiliation: Department of Chemistry-Biochemistry-Physics, University of Québec, Trois-Rivières, C.P. 500, TR (Quebec) G9A 5H7, Canada.
authors.forename: D
authors.initials: D
authors.lastname: Agudelo
authors.affiliation: Department of Chemistry-Biochemistry-Physics, University of Québec, Trois-Rivières, C.P. 500, TR (Quebec) G9A 5H7, Canada.
authors.forename: G
authors.initials: G
authors.lastname: Bérubé
authors.affiliation: Department of Chemistry-Biochemistry-Physics, University of Québec, Trois-Rivières, C.P. 500, TR (Quebec) G9A 5H7, Canada. Electronic address: tajmirri@uqtr.ca.
authors.forename: H A
authors.initials: HA
authors.lastname: Tajmir-Riahi
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000737:chemistry / Q000627:therapeutic use; D002352:Carrier Proteins / Q000737:chemistry / Q000627:therapeutic use; D002417:Cattle; D004317:Doxorubicin / Q000737:chemistry / Q000627:therapeutic use*; D016503:Drug Delivery Systems; D006801:Humans; D007782:Lactoglobulins / Q000737:chemistry / Q000627:therapeutic use*; D009369:Neoplasms / Q000188:drug therapy*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000627:therapeutic use*
chemical_list: D001798:Blood Proteins; D002352:Carrier Proteins; D007782:Lactoglobulins; D012710:Serum Albumin, Bovine; D004317:Doxorubicin
keywords: Doxorubicin; Drug delivery; Hydrophobicity; Modeling; Protein; Spectroscopy