title: Hydrophilic antioxidants from Andean tomato landraces assessed by their bioactivities in vitro and in vivo.
abstract: Potential nutraceutical properties of hydrophilic antioxidants in fruits of tomato landraces collected in Andean valleys were characterised. Antioxidant metabolites were measured by HPLC-DAD-MS/MS in mature fruits and their biological activities were assessed by in vitro and in vivo methods. In vitro antioxidant capacities were established by TEAC and FRAP methods. For in vivo biological activities we used a procedure based on Caenorhabditis elegans subjected to thermal stress. In addition, Saccharomyces cerevisiae was also used as a rapid screening system to evaluate tomato antioxidant capacity. All tomato accessions displayed significant differences regarding metabolic composition, biological activity and antioxidant capacity. Metabolite composition was associated with geographical origin and fruit size. Antioxidant activities showed significant association with phenolic compounds, such as caffeoylquinic acids, ferulic acid-O-hexosides and rutin. Combination of in vitro and in vivo methods applied here allowed evaluation of the variability in nutraceutical properties of tomato landraces, which could be applied to other fruits or food products.
authors.affiliation: Facultad de Ciencias Químicas - CIBICI, Universidad Nacional de Córdoba - CONICET, Ciudad Universitaria, 5000 Córdoba, Argentina; SECyT - ISIDSA/ICYTAC, Universidad Nacional de Córdoba - CONICET, Ciudad Universitaria, 5000 Córdoba, Argentina.
authors.forename: Romina D
authors.initials: RD
authors.lastname: Di Paola Naranjo
authors.affiliation: Facultad de Ciencias Químicas - CIBICI, Universidad Nacional de Córdoba - CONICET, Ciudad Universitaria, 5000 Córdoba, Argentina.
authors.forename: Santiago
authors.initials: S
authors.lastname: Otaiza
authors.affiliation: Facultad de Ciencias Químicas - CIBICI, Universidad Nacional de Córdoba - CONICET, Ciudad Universitaria, 5000 Córdoba, Argentina.
authors.forename: Alejandra C
authors.initials: AC
authors.lastname: Saragusti
authors.affiliation: SECyT - ISIDSA/ICYTAC, Universidad Nacional de Córdoba - CONICET, Ciudad Universitaria, 5000 Córdoba, Argentina.
authors.forename: Veronica
authors.initials: V
authors.lastname: Baroni
authors.affiliation: Facultad de Ciencias Químicas - CIBICI, Universidad Nacional de Córdoba - CONICET, Ciudad Universitaria, 5000 Córdoba, Argentina.
authors.forename: Andrea Del V
authors.initials: Adel V
authors.lastname: Carranza
authors.affiliation: Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo y CCT CONICET Mendoza, Mendoza, Argentina.
authors.forename: Iris E
authors.initials: IE
authors.lastname: Peralta
authors.affiliation: Instituto de Biología Molecular de Rosario, CONICET, Universidad Nacional de Rosario, Rosario, Argentina.
authors.forename: Estela M
authors.initials: EM
authors.lastname: Valle
authors.affiliation: Instituto de Biotecnología, INTA, Castelar, Argentina.
authors.forename: Fernando
authors.initials: F
authors.lastname: Carrari
authors.affiliation: Facultad de Ciencias Químicas - CIBICI, Universidad Nacional de Córdoba - CONICET, Ciudad Universitaria, 5000 Córdoba, Argentina. Electronic address: rasis@fcq.unc.edu.ar.
authors.forename: Ramón
authors.initials: R
authors.lastname: Asis
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D017173:Caenorhabditis elegans / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000032:analysis / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry*; D018551:Solanum lycopersicum / Q000737:chemistry*; D011801:Quinic Acid / Q000031:analogs & derivatives / Q000032:analysis / Q000494:pharmacology; D012431:Rutin / Q000032:analysis / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D013020:South America; D053719:Tandem Mass Spectrometry
chemical_list: D000975:Antioxidants; D003373:Coumaric Acids; C472707:caffeoylquinic acid; D011801:Quinic Acid; D012431:Rutin; C004999:ferulic acid
keywords: Antioxidant capacity; Caenorhabditis elegans; Phenolic profile; Saccharomyces cerevisiae; Solanum lycopersicum; Tomato landraces