title: Apolipoprotein A1-Unique Peptide as a Diagnostic Biomarker for Acute Ischemic Stroke.
abstract: Clinically-informative biomarkers of ischemic stroke are needed for rapid diagnosis and timely treatment. In the present study, APOA1 unique peptide (APOA1-UP), a novel peptide biomarker, was identified and quantified by multiple reaction monitoring (MRM) using labeled reference peptide (LRP). Serum samples of 94 patients in the ischemic stroke group and 37 patients in the non-stroke group were analyzed for the levels of total APOA1-UP, low density lipoprotein cholesterol (LDL-C), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and total cholesterol (TC). Median ratio of total APOA1-UP/LRP was 2.14 (interquartile range, 0.40) in the non-stroke group and 1.32 (0.44) in the ischemic stroke group (p < 0.0001). The serum level of total APOA1-UP was independently correlated with the presence of ischemic stroke by multivariate logistic regression analysis (p < 0.0001). From the receiver operating characteristic (ROC) curve, the area under the curve (AUC) was 0.9750 and the optimal cutoff value of the serum APOA1-UP level was 1.80, which yielded a sensitivity of 90.63% and a specificity of 97.14%. The diagnostic efficiency of HDL-C was lower, with an AUC of 0.7488. Therefore, the serum level of APOA1-UP is a diagnostic biomarker candidate for ischemic stroke in the early stage.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China. z.x41@mail.scut.edu.cn.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhao
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China. yueyu9023@gmail.com.
authors.forename: Yue
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of China Medical University, Shenyang 110001, China. xwlcmu06@sina.com.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Xu
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of China Medical University, Shenyang 110001, China. np-2813@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Dong
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China. yuanyuan03_03@163.com.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China. binggao@scut.edu.cn.
authors.forename: Bing
authors.initials: B
authors.lastname: Gao
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of China Medical University, Shenyang 110001, China. tyjk1972@163.com.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Li
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China. wtzhang9@gmail.com.
authors.forename: Wentao
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D016632:Apolipoprotein A-I / Q000097:blood*; D019540:Area Under Curve; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D005260:Female; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D012372:ROC Curve; D020521:Stroke / Q000175:diagnosis* / Q000378:metabolism; D014280:Triglycerides / Q000097:blood
chemical_list: D016632:Apolipoprotein A-I; D015415:Biomarkers; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D014280:Triglycerides; D002784:Cholesterol
keywords: acute ischemic stroke (AIS); apolipoprotein A1 unique peptide (APOA1-UP); biomarker; high-density lipoprotein cholesterol (HDL-C); labeled reference peptide (LRP); multiple reaction monitoring (MRM)