title: Selective isolation of components from natural volatile oil by countercurrent chromatography with cyclodextrins as selective reagent.
abstract: Selective separation of chemical components from seven kinds of volatile oil by countercurrent chromatography with three types of cyclodextrins as selective reagent was investigated in this work. Preparative separation of chemical components from volatile oil is generally quite challenging due to their extremely complexity of the composition. A biphasic solvent system n-hexane-0.10 mol L(-1) cyclodextrin (1:1, v/v) was selected for separation of components from volatile oil and three types of cyclodextrins were investigated, including β-cyclodexrin, methyl-β-cyclodexrin and hydroxypropyl-β-cyclodexrin. All kinds of volatile oils are from seven kinds of traditional Chinese herb. Results showed that some chemical components could be well separated with high purity from each kind of volatile oil using different type of cyclodextrin as selective reagent. For example, germacrone and curcumenone could be selectively separated from volatile oil of Curcumae Rhizoma with methyl-β-cyclodexrin and hydroxypropyl-β-cyclodexrin as selector respectively, and other five components were selectively separated from volatile oil of Chuanxiong Rhizoma, Myristicae Semen, Aucklandiae Radix and Angelicae Sinensis Radix by countercurrent chromatography with different cyclodexrin as selective reagent. Separation mechanism for separation of components from volatile oil by countercurrent chromatography with cyclodextrin as selective reagent was proposed. Peak resolution of the present separation method could be greatly influenced by the chemical compositions of volatile oil.
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310032, China. Electronic address: sqtong@zjut.edu.cn.
authors.forename: Shengqiang
authors.initials: S
authors.lastname: Tong
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310032, China.
authors.forename: Mengxia
authors.initials: M
authors.lastname: Lu
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310032, China.
authors.forename: Chu
authors.initials: C
authors.lastname: Chu
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310032, China.
authors.forename: Jizhong
authors.initials: J
authors.lastname: Yan
authors.affiliation: Zhejiang Province Institute of Veterinary Drug and Feed Control, Hangzhou, 310020, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Huang
authors.affiliation: Zhejiang Province Institute of Veterinary Drug and Feed Control, Hangzhou, 310020, China.
authors.forename: Yongfei
authors.initials: Y
authors.lastname: Ying
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D003377:Countercurrent Distribution; D030024:Curcuma / Q000737:chemistry; D003505:Cyclodextrins / Q000737:chemistry*; D006586:Hexanes / Q000737:chemistry; D009822:Oils, Volatile / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry
chemical_list: D003505:Cyclodextrins; D006586:Hexanes; D009822:Oils, Volatile; C026385:n-hexane
keywords: Countercurrent chromatography; Cyclodextrins; Selective isolation; Separation mechanism; Volatile oil