title: Relationship between virulence factors, resistance to antibiotics and phylogenetic groups of uropathogenic Escherichia coli in two locations in Mexico.
abstract: BACKGROUND
  Escherichia coli is the major causative agent of urinary tract infections (UTI), and virulence factors are responsible for the severity of these emerging infections. The aim of this study was to evaluate the relationship between virulence determinants and antibiotic susceptibility with phylogenetic groups of E.coli isolates of UTI in two locations in Mexico.
  
  
  METHODS
  An analysis was performed on 50 isolates of E.coli from the centre of the country and 57 from a town in the southwest. The isolates were characterized by phenotype (serotyping assays, in vitro adhesion, biofilm formation, production of haemolysin, and antibiotic susceptibility) and genotype (phylogenetic groups and virulence genes).
  
  
  RESULTS
  In the centre of the country location the phylogenetic group B2 (60%) and F (12%) were significantly more prevalent and had a higher frequency of genes, fimH (96%), iutA (66%), sat (36%), compared to the southwest location, where the group A (35%) and B1 (21%) were significantly predominant and had fewer virulence genes. About one-fifth (21.5%) of all isolates belonged to the O25-ST131 group. Haemolysin and biofilm producing strains were significantly higher in the southwest location. Resistance to ampicillin (92.5%), tetracycline (76.6%), and trimethoprim/sulfamethoxazole (70.1%) were the most common in both groups.
  
  
  CONCLUSIONS
  The phylogenetic group, virulence factors, and antibiotic susceptibility of the E.coli that causes UTI in the community, varies significantly among the Mexican populations studied. Phylogenetic groups A and B1 may be multidrug resistant and have the ability to produce UTI.
authors.affiliation: Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo de los Bravo, Guerrero, México.
authors.forename: Laura Iveth
authors.initials: LI
authors.lastname: Miranda-Estrada
authors.affiliation: Laboratorio Clínico, Clínica Hospital ISSSTE, Chilpancingo de los Bravo, Guerrero, México.
authors.forename: María
authors.initials: M
authors.lastname: Ruíz-Rosas
authors.affiliation: Laboratorio de Patogenicidad Bacteriana, Departamento de Salud Pública, Facultad de Medicina, UNAM, Ciudad de México, D.F., México; Hospital Infantil de México «Federico Gómez», Secretaría de Salud, Ciudad de México, D.F., México.
authors.forename: José
authors.initials: J
authors.lastname: Molina-López
authors.affiliation: Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo de los Bravo, Guerrero, México.
authors.forename: Isela
authors.initials: I
authors.lastname: Parra-Rojas
authors.affiliation: Laboratorio de Patogenicidad Bacteriana, Departamento de Salud Pública, Facultad de Medicina, UNAM, Ciudad de México, D.F., México; Hospital Infantil de México «Federico Gómez», Secretaría de Salud, Ciudad de México, D.F., México.
authors.forename: Edgar
authors.initials: E
authors.lastname: González-Villalobos
authors.affiliation: Laboratorio de Investigación en Microbiología, Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo de los Bravo, Guerrero, México. Electronic address: natividadcastro24@gmail.com.
authors.forename: Natividad
authors.initials: N
authors.lastname: Castro-Alarcón
mesh_terms: D024881:Drug Resistance, Bacterial; D006801:Humans; D008800:Mexico; D010802:Phylogeny; D014552:Urinary Tract Infections / Q000382:microbiology; D056689:Uropathogenic Escherichia coli / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000472:pathogenicity; D014774:Virulence; D037521:Virulence Factors
chemical_list: D037521:Virulence Factors
keywords: Antibiotic resistance; Escherichia coli uropatógena; Factores de virulencia; Grupos filogenéticos; Infección del tracto urinario; Phylogenetic groups; Resistencia a antibióticos; Urinary tract infection; Uropathogenic Escherichia coli; Virulence factors