title: An alternative method based on enzymatic fat hydrolysis to quantify volatile compounds in wheat bread crumb.
abstract: An alternative method to quantify 40 volatile compounds in wheat bread crumb is proposed. It consists of a Soxhlet extraction with a mixture of dichloromethane and diethyl ether containing lipases and a subsequent concentration with Vigreux column. It is the first time that lipases are added to transform the fat into free fatty acids and glycerol, which elute at the end of the chromatogram after the analytes, avoiding problems in the chromatography due to fat residues, such as dirtiness in the injector, column clogging or overlapping peaks. The extract is most easily analysed by GC/MS, using a standard addition method to correct matrix effect. The method was fully validated, with extraction efficiencies between 70% and 100% and precision RSD lower than 15%. The method was applied to a commercial crumb, with acetoin, phenylethyl alcohol and acetic acid as highly abundant compounds, which are considered main volatiles in crumb.
authors.affiliation: I.U. Cinquima, Analytical Chemistry Group, University of Valladolid, E-47011 Valladolid, Spain. Electronic address: joana.pico@uva.es.
authors.forename: Joana
authors.initials: J
authors.lastname: Pico
authors.affiliation: I.U. Cinquima, Analytical Chemistry Group, University of Valladolid, E-47011 Valladolid, Spain.
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Nozal
authors.affiliation: Food Technology Area, E.T.S. Ingenierías Agrarias, University of Valladolid, E-34071 Palencia, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Gómez
authors.affiliation: I.U. Cinquima, Analytical Chemistry Group, University of Valladolid, E-47011 Valladolid, Spain.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Bernal
mesh_terms: D001939:Bread / Q000032:analysis*; D005591:Chemical Fractionation / Q000379:methods*; D005223:Fats / Q000378:metabolism*; D005232:Fatty Acids, Volatile / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006868:Hydrolysis; D008049:Lipase / Q000378:metabolism; D014908:Triticum
chemical_list: D005223:Fats; D005232:Fatty Acids, Volatile; D008049:Lipase
keywords: Bread crumb aroma; Fat hydrolysis; Gas chromatography; Lipases; Soxhlet extraction