title: Association of NLK polymorphisms with intramuscular fat content and fatty acid composition traits in pigs.
abstract: Nemo-like protein kinase (NLK) is a key enzyme in the noncanonical Wnt signaling pathway and it is involved in adipogenesis. In this study, the associations of the polymorphisms of the NLK gene with intramuscular fat (IMF) content and fatty acid (FA) composition traits were analyzed in crossbred commercial pigs. Three single nucleotide polymorphisms (SNPs) of the porcine NLK gene were identified in 5'-flanking region and introns, consisting of g.403739_403764insdel, g.574462T>C and g.630426A>G. The NLK g.403739_403764insdel was significantly associated with IMF content and palmitoleic acid levels. No association of the NLK g.574462T>C SNP with IMF content was observed. However, this SNP was significantly associated with arachidic and eicosenoic acid levels. Moreover, the NLK g.630426A>G SNP was significantly associated with IMF content and the fatty composition of arachidic, linoleic, as well as polyunsaturated FA and ω6 FA levels. These results indicate the importance of porcine NLK as a candidate gene for porcine IMF content and fatty acid composition traits.
authors.affiliation: Human and Animal Biotechnology Program, Graduate School, Chiang Mai University, Chiang Mai 50200, Thailand; Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Bangkok 10900, Thailand.
authors.forename: Pantaporn
authors.initials: P
authors.lastname: Supakankul
authors.affiliation: Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address: supamitmekchay@gmail.com.
authors.forename: Supamit
authors.initials: S
authors.lastname: Mekchay
mesh_terms: D050156:Adipogenesis / Q000235:genetics; D000818:Animals; D004041:Dietary Fats / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis*; D005229:Fatty Acids, Monounsaturated / Q000032:analysis; D043371:Fatty Acids, Omega-6 / Q000032:analysis; D060005:Genotyping Techniques; D019787:Linoleic Acid / Q000032:analysis; D018482:Muscle, Skeletal / Q000737:chemistry; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D000069466:Red Meat / Q000032:analysis*; D013552:Swine; D060449:Wnt Signaling Pathway
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D043371:Fatty Acids, Omega-6; C008757:palmitoleic acid; D019787:Linoleic Acid; D017346:Protein Serine-Threonine Kinases; C572289:eicosenoic acid
keywords: Association; Fatty acid; Intramuscular fat; NLK; Pig