title: ICOS Expression as Immunologic Marker in Immune Activating Monoclonal Antibodies.
abstract: Compelling experimental evidences point to monitoring of the absolute number of circulating ICOS-positive T cells as an early predictive marker of clinical activity of anti-CTLA-4 antibodies in cancer patients. Here, we report available data focusing on this issue and operative procedures to detect ICOS expression on circulating peripheral blood mononuclear cells during CTLA-4 blockade therapy.
authors.affiliation: Medical Oncology and Immunotherapy, Istituto Toscano Tumori, University Hospital of Siena, Siena, Italy. r.danielli.e@ao-siena.toscana.it.|Division of Medical Oncology and Immunotherapy, Department of Oncology, University Hospital of Siena, Viale Mario Bracci n. 16, Siena, 53100, Italy. r.danielli.e@ao-siena.toscana.it.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Danielli
authors.affiliation: Medical Oncology and Immunotherapy, Istituto Toscano Tumori, University Hospital of Siena, Siena, Italy.
authors.forename: Ornella
authors.initials: O
authors.lastname: Cutaia
authors.affiliation: Medical Oncology and Immunotherapy, Istituto Toscano Tumori, University Hospital of Siena, Siena, Italy.
authors.forename: Carolina
authors.initials: C
authors.lastname: Fazio
authors.affiliation: Medical Oncology and Immunotherapy, Istituto Toscano Tumori, University Hospital of Siena, Siena, Italy.
authors.forename: Erika
authors.initials: E
authors.lastname: Bertocci
authors.affiliation: Medical Oncology and Immunotherapy, Istituto Toscano Tumori, University Hospital of Siena, Siena, Italy.
authors.forename: Ester
authors.initials: E
authors.lastname: Fonsatti
authors.affiliation: Medical Oncology and Immunotherapy, Istituto Toscano Tumori, University Hospital of Siena, Siena, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Maio
authors.affiliation: Medical Oncology and Immunotherapy, Istituto Toscano Tumori, University Hospital of Siena, Siena, Italy.
authors.forename: Luana
authors.initials: L
authors.lastname: Calabrò
mesh_terms: D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D002469:Cell Separation; D002478:Cells, Cultured; D006801:Humans; D060889:Inducible T-Cell Co-Stimulator Protein / Q000378:metabolism*; D000074324:Ipilimumab; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; C556692:ICOS protein, human; D060889:Inducible T-Cell Co-Stimulator Protein; D000074324:Ipilimumab
keywords: CTLA-4; Flow cytometry; ICOS; Immunomodulating antibodies; Solid tumors