title: Redox Indicator Mice Stably Expressing Genetically Encoded Neuronal roGFP: Versatile Tools to Decipher Subcellular Redox Dynamics in Neuropathophysiology.
abstract: Reactive oxygen species (ROS) and downstream redox alterations not only mediate physiological signaling but also neuropathology. For long, ROS/redox imaging was hampered by a lack of reliable probes. Genetically encoded redox sensors overcame this gap and revolutionized (sub)cellular redox imaging. Yet, the successful delivery of sensor-coding DNA, which demands transfection/transduction of cultured preparations or stereotaxic microinjections of each subject, remains challenging. By generating transgenic mice, we aimed to overcome limiting cultured preparations, circumvent surgical interventions, and to extend effectively redox imaging to complex and adult preparations.
  
  
  
  Our redox indicator mice widely express Thy1-driven roGFP1 (reduction-oxidation-sensitive green fluorescent protein 1) in neuronal cytosol or mitochondria. Negative phenotypic effects of roGFP1 were excluded and its proper targeting and functionality confirmed. Redox mapping by ratiometric wide-field imaging reveals most oxidizing conditions in CA3 neurons. Furthermore, mitochondria are more oxidized than cytosol. Cytosolic and mitochondrial roGFP1s reliably report cell endogenous redox dynamics upon metabolic challenge or stimulation. Fluorescence lifetime imaging yields stable, but marginal, response ranges. We therefore developed automated excitation ratiometric 2-photon imaging. It offers superior sensitivity, spatial resolution, and response dynamics.
  
  
  
  Redox indicator mice enable quantitative analyses of subcellular redox dynamics in a multitude of preparations and at all postnatal stages. This will uncover cell- and compartment-specific cerebral redox signals and their defined alterations during development, maturation, and aging. Cross-breeding with other disease models will reveal molecular details on compartmental redox homeostasis in neuropathology. Combined with ratiometric 2-photon imaging, this will foster our mechanistic understanding of cellular redox signals in their full complexity. Antioxid. Redox Signal. 25, 41-58.
authors.affiliation: 1 Institut für Neuro- und Sinnesphysiologie, Georg-August-Universität Göttingen , Universitätsmedizin, Göttingen, Germany .
authors.forename: Kerstin C
authors.initials: KC
authors.lastname: Wagener
authors.affiliation: 1 Institut für Neuro- und Sinnesphysiologie, Georg-August-Universität Göttingen , Universitätsmedizin, Göttingen, Germany .
authors.forename: Benedikt
authors.initials: B
authors.lastname: Kolbrink
authors.affiliation: 1 Institut für Neuro- und Sinnesphysiologie, Georg-August-Universität Göttingen , Universitätsmedizin, Göttingen, Germany .
authors.forename: Katharina
authors.initials: K
authors.lastname: Dietrich
authors.affiliation: 1 Institut für Neuro- und Sinnesphysiologie, Georg-August-Universität Göttingen , Universitätsmedizin, Göttingen, Germany .
authors.forename: Kathrin M
authors.initials: KM
authors.lastname: Kizina
authors.affiliation: 1 Institut für Neuro- und Sinnesphysiologie, Georg-August-Universität Göttingen , Universitätsmedizin, Göttingen, Germany .
authors.forename: Lukas S
authors.initials: LS
authors.lastname: Terwitte
authors.affiliation: 1 Institut für Neuro- und Sinnesphysiologie, Georg-August-Universität Göttingen , Universitätsmedizin, Göttingen, Germany .
authors.forename: Belinda
authors.initials: B
authors.lastname: Kempkes
authors.affiliation: 2 Institut für Neurophysiologie und Zelluläre Biophysik, Georg-August-Universität Göttingen , Universitätsmedizin, Göttingen, Germany .
authors.forename: Guobin
authors.initials: G
authors.lastname: Bao
authors.affiliation: 1 Institut für Neuro- und Sinnesphysiologie, Georg-August-Universität Göttingen , Universitätsmedizin, Göttingen, Germany .
authors.forename: Michael
authors.initials: M
authors.lastname: Müller
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D003600:Cytosol / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics; D051379:Mice; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000378:metabolism* / Q000473:pathology / Q000502:physiology; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction; D014162:Transfection
chemical_list: D017382:Reactive Oxygen Species; D049452:Green Fluorescent Proteins; D005978:Glutathione