title: The association of Hsp90 expression induced by aspirin with anti-stress damage in chicken myocardial cells.
abstract: The protective effect of aspirin during exposure to heat stress in broiler chickens was investigated. We assayed pathological damage, expression and distribution of Hsp90 protein and hsp90 mRNA expression in chicken heart tissues after oral administration of aspirin following exposure to high temperature for varying times. Heat stress induced increases in plasma aspartate aminotransferase, creatine kinase and lactate dehydrogenase activities while causing severe heart damage, which was characterized by granular and vacuolar degeneration, nuclear shrinkage and even myocardium fragmentation in cardiac muscle fibers. After aspirin administration, myocardial cells showed fewer pathological lesions than broilers treated with heat alone. A high positive Hsp90 signal was always detected in the nuclei of myocardial cells from broilers treated with aspirin, while in myocardial cells treated with heat alone, Hsp90 in the nuclei decreased, as did that in the cytoplasm. Aspirin induced rapid and significant synthesis of Hsp90 before and at the initial phase of heat stress, and significant expression of hsp90 mRNA was stimulated throughout the experiment when compared with cells exposed to heat stress alone. Thus, specific pre-induction of Hsp90 in cardiovascular tissue was useful for resisting heat stress damage because it produced stable damage-related enzymes and fewer pathologic changes.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Huai-sen
authors.initials: HS
authors.lastname: Zhu
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Qian
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Tang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.affiliation: Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Hannover 30173, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Kemper
authors.affiliation: Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Hannover 30173, Germany.
authors.forename: Joerg
authors.initials: J
authors.lastname: Hartung
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: En-dong
authors.initials: ED
authors.lastname: Bao
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology*; D002467:Cell Nucleus / Q000235:genetics; D002645:Chickens; D005786:Gene Expression Regulation / Q000187:drug effects*; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics*; D006358:Hot Temperature; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D013312:Stress, Physiological / Q000187:drug effects*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D018841:HSP90 Heat-Shock Proteins; D001241:Aspirin
keywords: Hsp90; aspirin; chicken; heart; heat stress damage