title: Inhibitory activity of extracts of Hebridean brown seaweeds on lipase activity.
abstract: The effect of three Hebridean brown seaweeds on lipase activity was assessed using a turbidimetric lipase activity assay and an in vitro simulation of the upper digestive tract. The preparations ofAscophyllum nodosum,Fucus vesiculosus, andPelvetia canaliculatawere tested; whole seaweed homogenate, sodium carbonate extract, and ethanol extracts (pellet and supernatant were tested separately). All extracts showed significant inhibition of lipase, suggesting multiple bioactive agents, potentially including alginates, fucoidans, and polyphenols. Whole homogenate extract ofF. vesiculosuswas the most potent inhibitor of Lipase (IC50= 0.119 mg mL-1), followed by ethanol supernatant (IC50= 0.159 mg mL-1) while ethanol pellet and sodium carbonate extract showed relatively weaker inhibition (IC50= 0.360 mg mL-1 and IC50= 0.969 mg mL-1 respectively). ForA. nodosumandP. canaliculata, strongest inhibition occurred with ethanol pellet (IC50= 0.238 and 0.228 mg mL-1, respectively). These inhibitory effects were validated in a model gut system. The data presented herein suggests the use of seaweed as a potential weight management tool is deserving of further investigation.
authors.affiliation: Institute for Cell and Molecular Biosciences (ICaMB), Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle upon Tyne, NE2 4HH UK.
authors.forename: Peter Ian
authors.initials: PI
authors.lastname: Chater
authors.affiliation: Institute for Cell and Molecular Biosciences (ICaMB), Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle upon Tyne, NE2 4HH UK.
authors.forename: Mathew
authors.initials: M
authors.lastname: Wilcox
authors.affiliation: Institute for Cell and Molecular Biosciences (ICaMB), Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle upon Tyne, NE2 4HH UK.
authors.forename: Paul
authors.initials: P
authors.lastname: Cherry
authors.affiliation: Institute for Cell and Molecular Biosciences (ICaMB), Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle upon Tyne, NE2 4HH UK.
authors.forename: Andrew
authors.initials: A
authors.lastname: Herford
authors.affiliation: Institute for Cell and Molecular Biosciences (ICaMB), Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle upon Tyne, NE2 4HH UK.
authors.forename: Suraiami
authors.initials: S
authors.lastname: Mustar
authors.affiliation: Human Nutrition Research Centre, School of Agriculture, Food and Rural Development, Agriculture Building, Kings Road, Newcastle upon Tyne, NE1 7RU UK.
authors.forename: Hannah
authors.initials: H
authors.lastname: Wheater
authors.affiliation: Nanyang Polytechnic, Food and Human Nutrition Department, Newcastle University Office, 180 Ang Mo Kio, Avenue 8, 569830 Singapore, Singapore.
authors.forename: Iain
authors.initials: I
authors.lastname: Brownlee
authors.affiliation: Human Nutrition Research Centre, School of Agriculture, Food and Rural Development, Agriculture Building, Kings Road, Newcastle upon Tyne, NE1 7RU UK.
authors.forename: Chris
authors.initials: C
authors.lastname: Seal
authors.affiliation: Institute for Cell and Molecular Biosciences (ICaMB), Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle upon Tyne, NE2 4HH UK.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Pearson
keywords: Alginate; Ascophyllum nodosum; Fat digestion; Fucus vesiculosus; Inhibition; Lipase; Obesity; Pelvetia canaliculata; Polyphenol