title: Knockdown of ANXA1 suppresses the biological behavior of human NSCLC cells in vitro.
abstract: Annexin A1 (ANXA1) is a member of the annexin superfamily. Previous studies have reported that ANXA1 is highly expressed in various types of malignant tumor; however, its role in the progression of non‑small cell lung cancer (NSCLC) remains to be fully clarified. The present study aimed to investigate the oncogenic role of ANXA1 in NSCLC cells in vitro. RNA interference was used to downregulate ANXA1 expression in A549 and H1299 cells using a small interfering RNA lentiviral vector. Subsequently, cell proliferation and migration were detected using Cell Counting kit‑8, clone formation, wound healing and Transwell chamber assays. Successful transfection was confirmed using fluorescence microscopy, which demonstrated that ANXA1 had been efficiently inhibited. ANXA1 knockdown suppressed the proliferation, migration and invasion of NSCLC cells. In conclusion, the present study provided evidence suggesting that ANXA1 may contribute to the growth and invasion of NSCLC cell lines, and ANXA1 may be exploited as an in vitro therapeutic target for the treatment of NSCLC.
authors.affiliation: Department of Pathology, School of Basic Medical Science, Guangzhou Medical University, Guangzhou, Guangdong 510180, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Department of Pathology, School of Basic Medical Science, Guangzhou Medical University, Guangzhou, Guangdong 510180, P.R. China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Guan
authors.affiliation: Department of Pathology, School of Basic Medical Science, Guangzhou Medical University, Guangzhou, Guangdong 510180, P.R. China.
authors.forename: Tonghui
authors.initials: T
authors.lastname: Cai
authors.affiliation: Department of Pathology, School of Basic Medical Science, Guangzhou Medical University, Guangzhou, Guangdong 510180, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Long
authors.affiliation: Department of Pathology, School of Basic Medical Science, Guangzhou Medical University, Guangzhou, Guangdong 510180, P.R. China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Pathology, School of Basic Medical Science, Guangzhou Medical University, Guangzhou, Guangdong 510180, P.R. China.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Xie
authors.affiliation: Department of Pathology, School of Basic Medical Science, Guangzhou Medical University, Guangzhou, Guangdong 510180, P.R. China.
authors.forename: Yajie
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000368:Aged; D017305:Annexin A1 / Q000235:genetics / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D016086:Lentivirus; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering
chemical_list: D017305:Annexin A1; D009363:Neoplasm Proteins; D034741:RNA, Small Interfering