title: Behaviour of the Biological Adhesives TachoSil®, GelitaSpon®, and a New Elastic Cyanoacrylate (Adhflex®) in Experimental Renal Trauma and Wound Healing.
abstract: BACKGROUND
  Renal injuries are relatively frequent in abdominal trauma. In some cases, adhesives and sealants can be used to repair and preserve injured organs. This paper describes the behaviour of three biomaterials - TachoSil®, GelitaSpon®, and a new elastic cyanoacrylate (CyA), Adhflex® - in standardized experimental renal injuries.
  
  
  METHODS
  Ninety male Wistar rats (300-350 g) were used. A Stiefel Biopsy Punch (8 mm diameter, 3 mm depth) was used to create injuries to the anterior kidney to evaluate wound healing. The animals were divided into five groups: (1) sham (n = 3); (2) control (n = 6), untreated, standard punch injury created on the anterior left kidney; (3) TachoSil® (n = 27), punch injury treated with TachoSil®; (4) GelitaSpon® (n = 27), punch injury treated with GelitaSpon®, and (5) Adhflex® (n = 27), punch injury treated with the new elastic CyA adhesive. The parameters studied were bleeding time, peritoneal adhesions, and histopathological evaluation of wound healing on days 2, 6, and 18, including measurements of the gap between wound edges, inflammatory reaction (CD68), and vascular neoformation (CD31).
  
  
  RESULTS
  The bleeding time was significantly shorter (27.7 ± 12.9 s) in the Adhflex® group than in the control (135.8 ± 11.6 s; p < 0.01), TachoSil® (77.5 ± 7.4 s; p < 0.05), and GelitaSpon® (82.5 ± 14.4 s; p < 0.05) groups. The incidence of intraperitoneal adhesions in the animals treated with Adhflex® was 3.6 times higher than in the non-treated group. It was also higher (p < 0.04) than in the groups treated with TachoSil® and GelitaSpon®. The time point with the largest gap between the wound edges and most abundant granulation tissue was at day 6. The largest gap after 18 days was reported for the Adhflex® adhesive. With regard to the markers CD31 and CD68, Adhflex® showed the largest areas 2 days after surgery, but no differences were found after 6 and 18 days versus the other treatments. The expression of the immunomarkers on the renal samples treated with Adhflex® was consistent with a normal healing process.
  
  
  CONCLUSIONS
  In this experimental model of renal injuries, the new elastic CyA (Adhflex®) resulted in the shortest bleeding time. It offers rapid sealing of the bleeding produced by renal injuries, fixation to adjacent tissues, and reduced occurrences of relapse. The evolution of the scarring is similar to other procedures. Given that traumatic renal injuries are always an emergency due to haemorrhage, Adhflex® might offer additional benefits over conventional treatment methods in human clinical practice.
authors.affiliation: Department of Surgery, Valencia University School of Medicine, Valencia, Spain.
authors.forename: José Miguel
authors.initials: JM
authors.lastname: Lloris-Carsí
authors.forename: David
authors.initials: D
authors.lastname: García-Cerdá
authors.forename: Beatriz
authors.initials: B
authors.lastname: Prieto-Moure
authors.forename: Carlos
authors.initials: C
authors.lastname: Barrios
authors.forename: Antonio B
authors.initials: AB
authors.lastname: Martín-Ballester
authors.forename: Dolores
authors.initials: D
authors.lastname: Cejalvo-Lapeña
mesh_terms: D000818:Animals; D001760:Bleeding Time; D002482:Cellulose / Q000494:pharmacology*; D003487:Cyanoacrylates / Q000494:pharmacology*; D004338:Drug Combinations; D005340:Fibrinogen / Q000494:pharmacology*; D007150:Immunohistochemistry; D007668:Kidney / Q000293:injuries* / Q000473:pathology; D016015:Logistic Models; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013917:Thrombin / Q000494:pharmacology*; D000267:Tissue Adhesions / Q000453:epidemiology; D014014:Tissue Adhesives / Q000494:pharmacology*; D014945:Wound Healing
chemical_list: D003487:Cyanoacrylates; D004338:Drug Combinations; C518955:TachoSil; D014014:Tissue Adhesives; D005340:Fibrinogen; D002482:Cellulose; D013917:Thrombin