title: LC-MS-MS Method for Analysis of Opiates in Wastewater During Football Games II.
abstract: Continuing our previous studies analyzing drugs of abuse in municipal wastewater, a method was developed for the analysis of opiates in wastewater samples using liquid chromatography coupled with tandem mass spectrometry (LC-MS-MS). Eight opiate drugs and metabolites were analyzed including codeine, hydrocodone, hydromorphone, 6-monoacetylmorphine (6-MAM, the primary urinary metabolite of heroin), morphine, norhydrocodone (the primary urinary metabolite of hydrocodone), oxycodone and oxymorphone. These drugs were chosen because of their widespread abuse. Wastewater samples were collected at both the Oxford Waste Water Treatment Plant in Oxford, Mississippi (MS) and the University Wastewater Treatment Plant in University, MS. These wastewater samples were collected on weekends in which the Ole Miss Rebel football team held home games (Vaught-Hemingway Stadium, University, MS 38677). The collected samples were analyzed using a validated method and found to contain codeine, hydrocodone, hydromorphone, morphine, norhydrocodone, oxycodone and oxymorphone. None of the samples contained 6-MAM.
authors.affiliation: ElSohly Laboratories, Inc., 5 Industrial Park Drive, Oxford, MS 38655, USA National Center for Natural Products Research, University of Mississippi, University, MS 38677, USA.
authors.forename: Waseem
authors.initials: W
authors.lastname: Gul
authors.affiliation: ElSohly Laboratories, Inc., 5 Industrial Park Drive, Oxford, MS 38655, USA Department of Chemistry and Biochemistry, University of Mississippi, University, MS 38677, USA.
authors.forename: Brandon
authors.initials: B
authors.lastname: Stamper
authors.affiliation: Department of Chemistry and Biochemistry, University of Mississippi, University, MS 38677, USA.
authors.forename: Murrell
authors.initials: M
authors.lastname: Godfrey
authors.affiliation: ElSohly Laboratories, Inc., 5 Industrial Park Drive, Oxford, MS 38655, USA.
authors.forename: Shahbaz W
authors.initials: SW
authors.lastname: Gul
authors.affiliation: ElSohly Laboratories, Inc., 5 Industrial Park Drive, Oxford, MS 38655, USA National Center for Natural Products Research, University of Mississippi, University, MS 38677, USA Department of Pharmaceutics and Drug Delivery, University of Mississippi, University, MS 38677, USA elsohly@elsohly.com.
authors.forename: Mahmoud A
authors.initials: MA
authors.lastname: ElSohly
mesh_terms: D000701:Analgesics, Opioid / Q000032:analysis* / Q000652:urine; D002853:Chromatography, Liquid; D003061:Codeine / Q000652:urine; D005538:Football; D006801:Humans; D006853:Hydrocodone / Q000652:urine; D004091:Hydromorphone / Q000652:urine; D009020:Morphine / Q000652:urine; D053610:Opiate Alkaloids / Q000032:analysis* / Q000652:urine; D010098:Oxycodone / Q000652:urine; D010111:Oxymorphone / Q000652:urine; D015813:Substance Abuse Detection / Q000379:methods*; D053719:Tandem Mass Spectrometry; D062065:Wastewater / Q000032:analysis / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000701:Analgesics, Opioid; D003061:Codeine; D006853:Hydrocodone; D004091:Hydromorphone; D009020:Morphine; D053610:Opiate Alkaloids; D010098:Oxycodone; D010111:Oxymorphone; D062065:Wastewater; D014874:Water Pollutants, Chemical