title: High Salt Diet Affects Renal Sodium Excretion and ERRα Expression.
abstract: Kidneys regulate the balance of water and sodium and therefore are related to blood pressure. It is unclear whether estrogen-related receptor α (ERRα), an orphan nuclear receptor and transcription factor highly expressed in kidneys, affects the reabsorption of water and sodium. The aim of this study was to determine whether changes in the expressions of ERRα, Na⁺/K⁺-ATPase and epithelial sodium channel (ENaC) proteins affected the reabsorption of water and sodium in kidneys of Dahl salt-sensitive (DS) rats. SS.13BN rats, 98% homologous to the DS rats, were used as a normotensive control group. The 24 h urinary sodium excretion of the DS and SS.13BN rats increased after the 6-week high salt diet intervention, while sodium excretion was increased in DS rats with daidzein (agonist of ERRα) treatment. ERRα expression was decreased, while β- and γ-ENaC mRNA expressions were increased upon high sodium diet treatment in the DS rats. In the chromatin immunoprecipitation (CHIP) assay, positive PCR signals were obtained in samples treated with anti-ERRα antibody. The transcriptional activity of ERRα was decreased upon high salt diet intervention. ERRα reduced the expressions of β- and γ-ENaC by binding to the ENaC promoter, thereby increased Na+ reabsorption. Therefore, ERRα might be one of the factors causing salt-sensitive hypertension.
authors.affiliation: Department of Cardiovascular Medicine, First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Key Laboratory of Molecular Cardiology, No. 277 Yanta West Road, Xi'an 710061, China. m15991631129@163.com.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Cardiovascular Medicine, First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Key Laboratory of Molecular Cardiology, No. 277 Yanta West Road, Xi'an 710061, China. wangyangxxk@126.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Cardiovascular Medicine, First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Key Laboratory of Molecular Cardiology, No. 277 Yanta West Road, Xi'an 710061, China. Liufuqiang0909@163.com.
authors.forename: Fu-Qiang
authors.initials: FQ
authors.lastname: Liu
authors.affiliation: Department of Cardiovascular Medicine, First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Key Laboratory of Molecular Cardiology, No. 277 Yanta West Road, Xi'an 710061, China. zuyiyuan@mail.xjtu.edu.cn.
authors.forename: Zu-Yi
authors.initials: ZY
authors.lastname: Yuan
authors.affiliation: Department of Cardiovascular Medicine, First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Key Laboratory of Molecular Cardiology, No. 277 Yanta West Road, Xi'an 710061, China. mujjun1964@163.com.
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Mu
mesh_terms: D000818:Animals; D053503:Epithelial Sodium Channels / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D007529:Isoflavones / Q000008:administration & dosage / Q000494:pharmacology; D007668:Kidney / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D051381:Rats; D020303:Rats, Inbred Dahl; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism*; D012964:Sodium / Q000652:urine*; D017673:Sodium Chloride, Dietary / Q000008:administration & dosage* / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000378:metabolism; D000097983:ERRalpha Estrogen-Related Receptor
chemical_list: D053503:Epithelial Sodium Channels; D007529:Isoflavones; D011960:Receptors, Estrogen; D012964:Sodium; D017673:Sodium Chloride, Dietary; D000254:Sodium-Potassium-Exchanging ATPase; D000097983:ERRalpha Estrogen-Related Receptor; C004742:daidzein
keywords: estrogen-related receptor α; potassium; salt sensitive; sodium