title: Hydrogen sulfide mediates nicotine biosynthesis in tobacco (Nicotiana tabacum) under high temperature conditions.
abstract: Hydrogen sulfide (H2S) acts as a signal to induce many physiological processes in plants, but its role in controlling the biosynthesis of secondary metabolites is not well established. In this study, we found that high temperature (HT) treatment induced nicotine biosynthesis in tobacco (Nicotiana tabacum) and promoted the rapid accumulation of H2S. Furthermore, HT triggered the biosynthesis of jasmonic acid (JA), a plant hormone that promotes nicotine biosynthesis. Suppression of the H2S signal using chemical inhibitors or via RNAi suppression of l-cysteine desulphydrase (L-CD) in transgenic plants, compromised JA production and nicotine biosynthesis under HT treatments, and these inhibitory effects could be reversed by applying exogenous H2S. Based on these data, we propose that H2S is an important trigger of nicotine biosynthesis in tobacco under HT conditions, and that H2S acts upstream of JA signaling by modulating the transcription of genes associated with JA biosynthesis.
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai, China.
authors.forename: Ruijing
authors.initials: R
authors.lastname: Li
authors.affiliation: Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai, China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Plant Production Department, College of Food and Agricultural Sciences, King Saud University, PO. Box. 2460 Riyadh 11451, Saudi Arabia.
authors.forename: Abd Allah
authors.initials: AA
authors.lastname: Ef
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Aiqun
authors.initials: A
authors.lastname: Jia
authors.affiliation: Laboratory of Tobacco Agriculture, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China. Electronic address: liwei.hu@gmail.com.
authors.forename: Liwei
authors.initials: L
authors.lastname: Hu
authors.affiliation: Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai, China. Electronic address: huxiangyang@kib.ac.cn.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Hu
mesh_terms: D053898:Biosynthetic Pathways / Q000187:drug effects; D003517:Cyclopentanes / Q000378:metabolism; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D009538:Nicotine / Q000096:biosynthesis*; D054883:Oxylipins / Q000378:metabolism; D030821:Plants, Genetically Modified; D034622:RNA Interference / Q000187:drug effects; D013696:Temperature; D014026:Nicotiana / Q000187:drug effects / Q000378:metabolism*
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; D009538:Nicotine; C011006:jasmonic acid; D006862:Hydrogen Sulfide
keywords: H(2)S; High temperature; Jasmonic acid; Nicotine