title: Proteomic profile of platelets during reconstitution of platelet counts after apheresis.
abstract: Circulating platelets consist of subpopulations of different age. We designed an approach to remove platelets from circulation using platelet apheresis. We aimed to detect changes in the platelet proteome related to increased platelet turnover after apheresis to map candidate proteins, which may serve as markers of young platelets.
  
  
  
  A healthy donor underwent three platelet apheresis procedures on three consecutive days. Blood was drawn at day 1 (baseline) and at days 3, 5, 7, 9, and 11 for the analysis of the platelet proteome using LC-ESI-MS/MS and 2D-DIGE.
  
  
  
  Among 1017 proteins identified by LC-ESI-MS/MS, 54 changed in quantity throughout the course of the study. Potential markers of young platelets were 40S ribosomal protein SA, COP9 signalosome complex subunit 5, and proteins involved in clathrin-mediated endocytosis signaling. Among 1036 protein spots observed by 2D-DIGE, 45 spots displayed changes in fluorescence intensity. Identified spots contained IQ motif containing GTPase activating protein 2, talin, moesin, myosin regulatory light chain 2, and coronin-1C.
  
  
  
  We provide the proof of principle that a combination of platelet apheresis and proteomic approaches enables identification of changes in the platelet proteome that are related to platelet de novo synthesis.
authors.affiliation: Institut für Immunologie und Transfusionsmedizin, University Medicine Greifswald, Greifswald, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Thiele
authors.affiliation: Institut für Immunologie und Transfusionsmedizin, University Medicine Greifswald, Greifswald, Germany.|Interfakultäres Institut für Genetik und Funktionelle Genomforschung, University Medicine Greifswald, Greifswald, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Braune
authors.affiliation: Interfakultäres Institut für Genetik und Funktionelle Genomforschung, University Medicine Greifswald, Greifswald, Germany.
authors.forename: Vishnu
authors.initials: V
authors.lastname: Dhople
authors.affiliation: Interfakultäres Institut für Genetik und Funktionelle Genomforschung, University Medicine Greifswald, Greifswald, Germany.
authors.forename: Elke
authors.initials: E
authors.lastname: Hammer
authors.affiliation: Klinik und Poliklinik für Hals-Nasen-Ohrenkrankheiten, Kopf- und Halschirurgie, University Medicine Greifswald, Greifswald, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Scharf
authors.affiliation: Institut für Immunologie und Transfusionsmedizin, University Medicine Greifswald, Greifswald, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Greinacher
authors.affiliation: Interfakultäres Institut für Genetik und Funktionelle Genomforschung, University Medicine Greifswald, Greifswald, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Völker
authors.affiliation: Interfakultäres Institut für Genetik und Funktionelle Genomforschung, University Medicine Greifswald, Greifswald, Germany.
authors.forename: Leif
authors.initials: L
authors.lastname: Steil
mesh_terms: D001781:Blood Component Removal; D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism*; D016000:Cluster Analysis; D006801:Humans; D010976:Platelet Count; D025341:Principal Component Analysis; D040901:Proteomics
keywords: Platelet age; Platelet apheresis; Platelet proteomics; Platelet turnover