title: Rice bacterial endophytes: isolation of a collection, identification of beneficial strains and microbiome analysis.
abstract: Endophytes are harmless or beneficial microorganisms that live inside plants between cells. The relationship they develop with the plant as well as their potential role in plant health is at large unexplored and it is believed that the opportunity to find new and interesting endophytes among the large variety of plants is great. Here, we present the isolation and analysis of a large collection of endophytes from one cultivar of rice grown in Italy. A total 1318 putative endophytes were isolated from roots, leaves and stems from rice grown in submerged and dry conditions and a working collection of 229 isolates was created. Among these, several isolates were confirmed to be endophytes and a few displayed the trait of plant growth promotion. A cultivation independent analysis via 16S rDNA amplicons of the bacterial community of the endosphere was also performed providing information on bacterial diversity in the rice endopshere.
authors.affiliation: International Centre for Genetic Engineering and Biotechnology, Padriciano 99, 34149, Trieste, Italy.
authors.forename: Iris
authors.initials: I
authors.lastname: Bertani
authors.affiliation: Parco Tecnologico Padano (PTP) via A. Einstein Loc. Codazza, 26900, Lodi, Italy.
authors.forename: Pamela
authors.initials: P
authors.lastname: Abbruscato
authors.affiliation: Parco Tecnologico Padano (PTP) via A. Einstein Loc. Codazza, 26900, Lodi, Italy.
authors.forename: Pietro
authors.initials: P
authors.lastname: Piffanelli
authors.affiliation: International Centre for Genetic Engineering and Biotechnology, Padriciano 99, 34149, Trieste, Italy.
authors.forename: Sujatha
authors.initials: S
authors.lastname: Subramoni
authors.affiliation: International Centre for Genetic Engineering and Biotechnology, Padriciano 99, 34149, Trieste, Italy.
authors.forename: Vittorio
authors.initials: V
authors.lastname: Venturi
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D060026:Endophytes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D007558:Italy; D064307:Microbiota; D012275:Oryza / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology; D018547:Plant Stems / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S