title: Surface Plasmon Resonance based sensing of lysozyme in serum on Micrococcus lysodeikticus-modified graphene oxide surfaces.
abstract: Lysozyme is an enzyme found in biological fluids, which is upregulated in leukemia, renal diseases as well as in a number of inflammatory gastrointestinal diseases. We present here the development of a novel lysozyme sensing concept based on the use of Micrococcus lysodeikticus whole cells adsorbed on graphene oxide (GO)-coated Surface Plasmon Resonance (SPR) interfaces. M. lysodeikticus is a typical enzymatic substrate for lysozyme. Unlike previously reported sensors which are based on the detection of lysozyme through bioaffinity interactions, the bioactivity of lysozyme will be used here for sensing purposes. Upon exposure to lysozyme containing serum, the integrity of the bacterial cell wall is affected and the cells detach from the GO based interfaces, causing a characteristic decrease in the SPR signal. This allows sensing the presence of clinically relevant concentrations of lysozyme in undiluted serum samples.
authors.affiliation: International Center of Biodynamics, 1B Intrarea Portocalelor, Sector 6, 060101 Bucharest, Romania. Electronic address: avasilescu@biodyn.ro.
authors.forename: Alina
authors.initials: A
authors.lastname: Vasilescu
authors.affiliation: International Center of Biodynamics, 1B Intrarea Portocalelor, Sector 6, 060101 Bucharest, Romania.
authors.forename: Szilveszter
authors.initials: S
authors.lastname: Gáspár
authors.affiliation: International Center of Biodynamics, 1B Intrarea Portocalelor, Sector 6, 060101 Bucharest, Romania.
authors.forename: Mihaela
authors.initials: M
authors.lastname: Gheorghiu
authors.affiliation: International Center of Biodynamics, 1B Intrarea Portocalelor, Sector 6, 060101 Bucharest, Romania.
authors.forename: Sorin
authors.initials: S
authors.lastname: David
authors.affiliation: National Institute for Laser, Plasma and Radiation Physics (INFLPR), Str Atomistilor 409, PO Box MG-36, 077125 Magurele, Bucharest, Romania.
authors.forename: Valentina
authors.initials: V
authors.lastname: Dinca
authors.affiliation: Department of Chemical Engineering and Materials Science, Michigan State University, 428 s Shaw Lane, East Lansing, Michigan 48824-1226, United States.
authors.forename: Serban
authors.initials: S
authors.lastname: Peteu
authors.affiliation: Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR CNRS 8520, Lille1 University, Avenue Poincaré-BP60069, 59652 Villeneuve d'Ascq, France; Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials (Ministry of Education), Shandong University, Jinan 250061, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials (Ministry of Education), Shandong University, Jinan 250061, China.
authors.forename: Musen
authors.initials: M
authors.lastname: Li
authors.affiliation: Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR CNRS 8520, Lille1 University, Avenue Poincaré-BP60069, 59652 Villeneuve d'Ascq, France.
authors.forename: Rabah
authors.initials: R
authors.lastname: Boukherroub
authors.affiliation: Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR CNRS 8520, Lille1 University, Avenue Poincaré-BP60069, 59652 Villeneuve d'Ascq, France. Electronic address: sabine.szunerits@univ-lille1.fr.
authors.forename: Sabine
authors.initials: S
authors.lastname: Szunerits
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D018914:Cells, Immobilized / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D016982:Micrococcus luteus / Q000378:metabolism*; D009113:Muramidase / Q000097:blood* / Q000378:metabolism*; D010087:Oxides / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D010087:Oxides; D006046:Gold; D006108:Graphite; D009113:Muramidase
keywords: Graphene oxide; Layer-by-layer; Lysozyme; Serum; Surface Plasmon Resonance; Whole cell biosensor