title: The role of 15-lipoxygenase-1 expression and its potential role in the pathogenesis of endometrial hyperplasia and endometrial adenocarcinomas.
abstract: OBJECTIVE
  To investigate the presence of 15-lipoxygenase-1 (15-LOX-1) expression and its potential role in the pathogenesis of endometrial hyperplasia and endometrial adenocarcinomas.
  
  
  METHODS
  The authors investigated the presence of 15-LOX-1 expression in samples from patients diagnosed with normal endometrium (n = 12), endometrial hyperplasia (n = 12), and endometrial cancer (n = 12). The immunohistochemical stainings were scored by three independent pathologists. A Western blot of 15- LOX-1 determined the presence of protein expression in normal endometrium. A Kolmogorov-Smirnov test was used to evaluate the data's distribution pattern. For pairwise comparisons of the combined scores between groups, the Mann-Whitney U test was used.
  
  
  RESULTS
  Based on the combined scores for 15-LOX-1 expression, strong immunochemistry staining was observed in samples diagnosed with normal endometrium. There was a significant difference in 15-LOX-1 expression between normal endometrium and endometrial adenocarcinoma (p = 0.03). Comparing tissues from normal endometrium and endometrial hyperplasia, there was a decline in the expression from normal endometrium to endometrial hyperplasia. However, the difference was not statistically significant.
  
  
  CONCLUSIONS
  The present results show that a decrease of 15-LOX-1 expression in the endometrial tumorigenesis process, starting from normal endometrium to hyperplasia and endometrial cancer, might be a trigger. Further studies are required to determine its potential use as a marker in a larger randomized multicenter study.
authors.forename: M E
authors.initials: ME
authors.lastname: Sak
authors.forename: I
authors.initials: I
authors.lastname: Alanbay
authors.forename: A
authors.initials: A
authors.lastname: Rodriguez
authors.forename: T
authors.initials: T
authors.lastname: Gokaslan
authors.forename: M
authors.initials: M
authors.lastname: Borahay
authors.forename: I
authors.initials: I
authors.lastname: Shureiqi
authors.forename: G S
authors.initials: GS
authors.lastname: Kilic
mesh_terms: D000328:Adult; D000368:Aged; D001093:Arachidonate 15-Lipoxygenase / Q000032:analysis / Q000502:physiology*; D004714:Endometrial Hyperplasia / Q000201:enzymology / Q000209:etiology*; D016889:Endometrial Neoplasms / Q000201:enzymology / Q000209:etiology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged
chemical_list: C515716:ALOX15 protein, human; D001093:Arachidonate 15-Lipoxygenase