title: Measles Virus Genotype D Wild Strains Suppress Interferon-Stimulated Gene Expression More Potently than Laboratory Strains in SiHa Cells.
abstract: Changes in interferon (IFN)-stimulated gene (ISG) expression in cells infected with measles virus (MeV), four wild strains (belonging to different genotypes), and the laboratory strain Edmonston were examined. ISGs [MxA, 2'-5'-oligoadenylate synthetase, and interferon regulatory factor-1] were upregulated in an MeV-infection-induced manner and in an IFN-induced manner. In MeV-infected SiHa cell lines, the MeV infection-induced expression levels were in the order of A>H1>D8>D5>D3. On the other hand, all infected cell lines abolished type I and III IFN-induced ISG expression. However, partial type II IFN-mediated induction was observed in the MeV-infected cells. The wild strain of genotype D3 was the most potent inhibitor of MeV infection-induced and IFN-induced ISG expression and generated the highest titer of infectious viral particles. Edmonston triggered the highest levels of MeV infection-induced ISG expression in SiHa cells and produced the lowest titer of infectious particles. Expression of the viral C protein was associated with suppression of MeV infection-induced and type II IFN-induced ISG expression.
authors.affiliation: 1 Department of Microbiology, Sapporo Medical University School of Medicine , Sapporo, Japan .
authors.forename: Masaru
authors.initials: M
authors.lastname: Jinushi
authors.affiliation: 1 Department of Microbiology, Sapporo Medical University School of Medicine , Sapporo, Japan .
authors.forename: Soh
authors.initials: S
authors.lastname: Yamamoto
authors.affiliation: 1 Department of Microbiology, Sapporo Medical University School of Medicine , Sapporo, Japan .|2 Department of Otorhinolaryngology, Sapporo Medical University School of Medicine , Sapporo, Japan .
authors.forename: Noriko
authors.initials: N
authors.lastname: Ogasawara
authors.affiliation: 3 Hokkaido Institute of Public Health , Sapporo, Japan .
authors.forename: Hideki
authors.initials: H
authors.lastname: Nagano
authors.affiliation: 4 Department of Pediatrics, Sapporo Medical University School of Medicine , Sapporo, Japan .
authors.forename: Shin
authors.initials: S
authors.lastname: Hashimoto
authors.affiliation: 4 Department of Pediatrics, Sapporo Medical University School of Medicine , Sapporo, Japan .
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsutsumi
authors.affiliation: 2 Department of Otorhinolaryngology, Sapporo Medical University School of Medicine , Sapporo, Japan .
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Himi
authors.affiliation: 1 Department of Microbiology, Sapporo Medical University School of Medicine , Sapporo, Japan .
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Yokota
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D045744:Cell Line, Tumor; D002584:Cervix Uteri / Q000187:drug effects / Q000276:immunology / Q000821:virology; D002522:Chlorocebus aethiops; D004847:Epithelial Cells / Q000187:drug effects / Q000276:immunology / Q000821:virology*; D005260:Female; D005786:Gene Expression Regulation; D016679:Genome, Viral; D005838:Genotype; D054884:Host-Pathogen Interactions; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D007372:Interferons / Q000494:pharmacology; D008459:Measles virus / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000276:immunology; D009570:Nitriles; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000276:immunology; D010957:Plasmids / Q000737:chemistry / Q000276:immunology; D011720:Pyrazoles / Q000494:pharmacology; D011743:Pyrimidines; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014709:Vero Cells; D019562:Viral Load / Q000187:drug effects; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000276:immunology*; D007378:Interleukins; D000095022:Interferon Lambda
chemical_list: D016898:Interferon-alpha; D007371:Interferon-gamma; D007372:Interferons; D009570:Nitriles; D019590:Nucleocapsid Proteins; D011720:Pyrazoles; D011743:Pyrimidines; D017361:Viral Nonstructural Proteins; C584392:C protein, measles virus; C000624353:interferon-lambda, human; D007378:Interleukins; D000095022:Interferon Lambda; C540383:ruxolitinib