title: Therapy to target renal cell carcinoma using 131I-labeled B7-H3 monoclonal antibody.
abstract: B7-H3 is a tumor-associated antigen that plays a critical role in potential tumor-targeted therapy. In this study, we aimed to assess the radiobiological effect of 131I-labeled B7-H3 monoclonal antibody (131I-4H7) in nude mice with human renal cell carcinoma (RCC) and evaluate the effect of 131I-4H7 on RCC treatment. The radiobiological activity and tumor uptake of 131I-4H7, and its effect on tumor growth were measured. 131I-4H7 was absorbed by the tumor and reached its maximal uptake rate (3.32% injected dose [ID]/g) at 24 h, at which point the drug concentration in the tumor was 7.36-, 2.06-, 1.80-, and 2.78-fold higher than that in muscle, kidneys, liver, and heart, respectively. Measurements and positron emission tomography-computed tomography imaging showed that tumor development was significantly inhibited by 131I-4H7. HE staining revealed that 131I-4H7 significantly injures tumor cells. Our results suggest that 131I-4H7 is markedly absorbed by the tumor and did suppress the development of RCC xenografted tumors in nude mice, which might provide a new candidate for antibody-mediated targeted radiotherapy in human RCC.
authors.affiliation: Department of Urology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu, China.
authors.forename: Gongcheng
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Urology, Huai'an Hospital Affiliated of Xuzhou Medical College and Huai'an Second People's Hospital, Huai'an, Jiangsu, China.
authors.forename: Ziyu
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Urology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu, China.
authors.forename: Yunyan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Gerontology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu, China.
authors.forename: Xueqin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Clinical Immunology Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
authors.forename: Guangbo
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Urology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
authors.forename: Jianquan
authors.initials: J
authors.lastname: Hou
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002292:Carcinoma, Renal Cell / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes / Q000008:administration & dosage / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008819:Mice, Nude
chemical_list: D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes
keywords: B7-H3; mice, nude; monoclonal antibody; renal cell carcinoma; targeting therapy