title: Direct and cost-efficient hyperpolarization of long-lived nuclear spin states on universal (15)N2-diazirine molecular tags.
abstract: Conventional magnetic resonance (MR) faces serious sensitivity limitations which can be overcome by hyperpolarization methods, but the most common method (dynamic nuclear polarization) is complex and expensive, and applications are limited by short spin lifetimes (typically seconds) of biologically relevant molecules. We use a recently developed method, SABRE-SHEATH, to directly hyperpolarize (15)N2 magnetization and long-lived (15)N2 singlet spin order, with signal decay time constants of 5.8 and 23 minutes, respectively. We find >10,000-fold enhancements generating detectable nuclear MR signals that last for over an hour. (15)N2-diazirines represent a class of particularly promising and versatile molecular tags, and can be incorporated into a wide range of biomolecules without significantly altering molecular function.
authors.affiliation: Department of Chemistry, Duke University, Durham, NC 27708, USA.
authors.forename: Thomas
authors.identifier: 0000-0001-6779-9978
authors.initials: T
authors.lastname: Theis
authors.affiliation: Department of Chemistry, Duke University, Durham, NC 27708, USA.
authors.forename: Gerardo X
authors.initials: GX
authors.lastname: Ortiz
authors.affiliation: Department of Chemistry, Duke University, Durham, NC 27708, USA.
authors.forename: Angus W J
authors.identifier: 0000-0003-3985-9835
authors.initials: AW
authors.lastname: Logan
authors.affiliation: Department of Physics, Duke University, Durham, NC 27708, USA.
authors.forename: Kevin E
authors.identifier: 0000-0002-6072-5033
authors.initials: KE
authors.lastname: Claytor
authors.affiliation: Department of Chemistry, Duke University, Durham, NC 27708, USA.
authors.forename: Yesu
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC 27708, USA.
authors.forename: William P
authors.identifier: 0000-0002-8815-4594
authors.initials: WP
authors.lastname: Huhn
authors.affiliation: Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC 27708, USA.
authors.forename: Volker
authors.initials: V
authors.lastname: Blum
authors.affiliation: Department of Chemistry, Duke University, Durham, NC 27708, USA.
authors.forename: Steven J
authors.identifier: 0000-0003-3229-0949
authors.initials: SJ
authors.lastname: Malcolmson
authors.affiliation: Departments of Radiology and Biomedical Engineering, Vanderbilt University, Institute of Imaging Science, Nashville, TN 37232, USA.
authors.forename: Eduard Y
authors.initials: EY
authors.lastname: Chekmenev
authors.affiliation: Department of Chemistry, Duke University, Durham, NC 27708, USA.
authors.forename: Qiu
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Duke University, Durham, NC 27708, USA.; Department of Physics, Duke University, Durham, NC 27708, USA.; Departments of Radiology and Biomedical Engineering, Duke University, Durham, NC 27708, USA.
authors.forename: Warren S
authors.initials: WS
authors.lastname: Warren
mesh_terms: D003978:Diazomethane / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D060526:Magnetic Fields; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D009587:Nitrogen Isotopes / Q000737:chemistry*
chemical_list: D009587:Nitrogen Isotopes; D003978:Diazomethane
keywords: Magnetic resonance; hyperpolarization; nuclear spin states