title: Detection of mcr-1 colistin resistance gene in polyclonal Escherichia coli isolates in Barcelona, Spain, 2012 to 2015.
abstract: Colistin resistance was detected in 53 of 10,011 Escherichia coli (0.5%) by prospective phenotypic testing of consecutive clinical isolates in a single hospital in Barcelona, Spain (2012-15). The mcr-1 gene was retrospectively identified by PCR and sequencing in 15 of 50 available isolates. Each isolate had a unique PFGE pattern except for two. This clonal diversity supports the hypothesis of horizontal dissemination of the mcr-1 gene in the local study population.
authors.affiliation: Microbiology Department, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
authors.forename: Núria
authors.initials: N
authors.lastname: Prim
authors.forename: Alba
authors.initials: A
authors.lastname: Rivera
authors.forename: Judith
authors.initials: J
authors.lastname: Rodríguez-Navarro
authors.forename: Montserrat
authors.initials: M
authors.lastname: Español
authors.forename: Miquel
authors.initials: M
authors.lastname: Turbau
authors.forename: Pere
authors.initials: P
authors.lastname: Coll
authors.forename: Beatriz
authors.initials: B
authors.lastname: Mirelis
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002648:Child; D003091:Colistin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000188:drug therapy* / Q000453:epidemiology; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D010641:Phenotype; D016133:Polymerase Chain Reaction; D015995:Prevalence; D011446:Prospective Studies; D013030:Spain / Q000453:epidemiology; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D003091:Colistin
keywords: Spain; antibiotic use; antimicrobial resistance; bacterial infections; laboratory surveillance; multidrug resistance