title: Aberrant localization of lamin B receptor (LBR) in cellular senescence in human cells.
abstract: 5-Bromodeoxyuridine (BrdU), a thymidine analogue, induces cellular senescence in mammalian cells. BrdU induces cellular senescence probably through the regulation of chromatin because BrdU destabilizes or disrupts nucleosome positioning and decondenses heterochromatin. Since heterochromatin is tethered to the nuclear periphery through the interaction with the nuclear envelope proteins, we examined the localization of the several nuclear envelope proteins such as lamins, lamin-interacting proteins, nuclear pore complex proteins, and nuclear transport proteins in senescent cells. We have shown here that lamin B receptor (LBR) showed a change in localization in both BrdU-induced and replicative senescent cells.
authors.affiliation: Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027, Japan.
authors.forename: Rumi
authors.initials: R
authors.lastname: Arai
authors.affiliation: Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027, Japan.
authors.forename: Atsuki
authors.initials: A
authors.lastname: En
authors.affiliation: Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Ukekawa
authors.affiliation: Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027, Japan; Ichiban Life Corporation, 1-1-7 Horai-cho, Naka-ku, Yokohama 231-0048, Japan.
authors.forename: Kensuke
authors.initials: K
authors.lastname: Miki
authors.affiliation: Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027, Japan. Electronic address: mifuji@yokohama-cu.ac.jp.
authors.forename: Michihiko
authors.initials: M
authors.lastname: Fujii
authors.affiliation: Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027, Japan; Ichiban Life Corporation, 1-1-7 Horai-cho, Naka-ku, Yokohama 231-0048, Japan.
authors.forename: Dai
authors.initials: D
authors.lastname: Ayusawa
mesh_terms: D001973:Bromodeoxyuridine / Q000494:pharmacology; D016922:Cellular Senescence / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D034882:Lamins / Q000378:metabolism*; D009685:Nuclear Envelope / Q000378:metabolism* / Q000648:ultrastructure*; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D014018:Tissue Distribution / Q000187:drug effects; D000097682:Lamin B Receptor
chemical_list: D001973:Bromodeoxyuridine; D034882:Lamins; D018160:Receptors, Cytoplasmic and Nuclear; D000097682:Lamin B Receptor
keywords: 5-Bromodeoxyuridine; Cellular senescence; Chromatin; LBR; Nuclear envelope