title: Analysis of Individual Signaling Complexes by mMAPS, a Flow-Proteometric System.
abstract: Signal transduction is essential for maintaining normal cell physiological functions, and deregulation of signaling can lead to diseases such as diabetes and cancers. Some of the major players in signal delivery are molecular complexes composed of proteins and nucleic acids. This unit describes a technique called microchannel for multiparameter analysis of proteins in a single complex (mMAPS) for analyzing and quantifying individual target signaling complexes. mMAPS is a flow-proteometric system that allows detection of individual proteins or complexes flowing through a microfluidic channel. Specific target proteins and nucleic acids labeled by fluorescent tags are harvested from tissues or cultured cells for analysis by the mMAPS system. Overall, mMAPS enables both detection of multiple components within a single complex and direct quantification of different populations of molecular complexes in one setting in a short timeframe and requiring very low sample input.
authors.affiliation: Department of Molecular and Cellular Oncology, University of Texas MD Anderson Cancer Center, Houston, Texas.|Graduate Institute for Cancer Biology and Center for Molecular Medicine, China Medical University, Taichung, Taiwan.|These authors contributed equally to this work.
authors.forename: Chao-Kai
authors.initials: CK
authors.lastname: Chou
authors.affiliation: Department of Molecular and Cellular Oncology, University of Texas MD Anderson Cancer Center, Houston, Texas.|These authors contributed equally to this work.
authors.forename: Pei-Hsiang
authors.initials: PH
authors.lastname: Tsou
authors.affiliation: Department of Molecular and Cellular Oncology, University of Texas MD Anderson Cancer Center, Houston, Texas.|Graduate Institute for Cancer Biology and Center for Molecular Medicine, China Medical University, Taichung, Taiwan.|Department of Biotechnology, Asia University, Taichung, Taiwan.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Hsu
authors.affiliation: Department of Molecular and Cellular Oncology, University of Texas MD Anderson Cancer Center, Houston, Texas.
authors.forename: Heng-Huan
authors.initials: HH
authors.lastname: Lee
authors.affiliation: Department of Molecular and Cellular Oncology, University of Texas MD Anderson Cancer Center, Houston, Texas.|Graduate Institute for Cancer Biology and Center for Molecular Medicine, China Medical University, Taichung, Taiwan.|Department of Biotechnology, Asia University, Taichung, Taiwan.
authors.forename: Ying-Nai
authors.initials: YN
authors.lastname: Wang
authors.affiliation: Department of Electrical and Computer Engineering, Texas A&M University, College Station, Texas.
authors.forename: Jun
authors.initials: J
authors.lastname: Kameoka
authors.affiliation: Department of Molecular and Cellular Oncology, University of Texas MD Anderson Cancer Center, Houston, Texas.|Graduate Institute for Cancer Biology and Center for Molecular Medicine, China Medical University, Taichung, Taiwan.|Department of Biotechnology, Asia University, Taichung, Taiwan.
authors.forename: Mien-Chie
authors.initials: MC
authors.lastname: Hung
mesh_terms: D000818:Animals; D002138:Calibration; D002478:Cells, Cultured; D006801:Humans; D046210:Microfluidic Analytical Techniques; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D011786:Quality Control
chemical_list: D011506:Proteins
keywords: flow proteometry; microfluidic; protein-protein interaction; protein−nucleic acid interaction; single complex; single molecule