title: Synergistic effects of ultraviolet-B and methyl jasmonate on tanshinone biosynthesis in Salvia miltiorrhiza hairy roots.
abstract: Tanshinones are major bioactive diterpenoids of Salvia miltiorrhiza roots used for the treatment of cardiocerebral diseases. To develop effective elicitation and bioprocess strategies for the enhanced production of tanshinones, ultraviolet-B (UV-B) irradiation and methyl jasmonate (MeJA) elicitation were applied alone or in combination respectively in S. miltiorrhiza hairy root cultures. Our results showed 40-min UV-B irradiation at 40μW/cm(2) stimulated tanshinone production without any suppression of root growth, suggesting a new effective elicitor to S. miltiorrhiza hairy root cultures for tanshinone production. Moreover, the combined treatment of UV-B irradiation and MeJA exhibited synergistic effects on the expression levels of 3-hydroxy-3-methylglutaryl-CoA reductase (SmHMGR) and geranylgeranyl diphosphate synthase (SmGGPPS) genes in the tanshinone biosynthetic pathway. When hairy roots of 18-day-old cultures were exposed to the combined elicitation for 9days, the maximum production of tanshinone reached to 28.21mg/L, a 4.9-fold increase over the control. The combined elicitation of UV-B and MeJA was firstly used to stimulate the production of biologically important secondary metabolites in hairy root cultures.
authors.affiliation: College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, China.
authors.forename: Cong Hui
authors.initials: CH
authors.lastname: Wang
authors.affiliation: Department of Horticulture, Soochow University, Suzhou 215123, China.
authors.forename: Li Ping
authors.initials: LP
authors.lastname: Zheng
authors.affiliation: College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, China; Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650205, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Tian
authors.affiliation: College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, China. Electronic address: jwwang@suda.edu.cn.
authors.forename: Jian Wen
authors.initials: JW
authors.lastname: Wang
mesh_terms: D045784:Abietanes / Q000378:metabolism*; D000085:Acetates / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism*; D054883:Oxylipins / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism*; D027561:Salvia miltiorrhiza / Q000187:drug effects* / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D045784:Abietanes; D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; C021751:tanshinone; C072239:methyl jasmonate
keywords: Elicitation; Hairy roots; MeJA; Salvia miltiorrhiza; Tanshinones; UV-B