title: Lower Detection Probability of Avian Plasmodium in Blood Compared to Other Tissues.
abstract: We tested whether the probability of detecting avian haemosporidia (Plasmodium and Haemoproteus) using molecular techniques differs among blood, liver, heart, and pectoral muscle tissues. We used a paired design, sampling the 4 tissue types in 55 individuals of a wild South American suboscine antbird, the white-shouldered fire-eye (Pyriglena leucoptera). We also identified parasites to cytochrome b lineage. Detection probability was significantly lower in blood compared to the other 3 tissue types combined. Eight of 22 infections were not detected in blood samples; 4-7 infections were not detected in the other individual tissues. The same parasite lineage was recovered from different tissues.
authors.affiliation: Departamento de Genética e Biologia Evolutiva, Universidade de São Paulo, Rua do Matão, 277, Cidade Universitária, São Paulo, São Paulo 05508-090, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Svensson-Coelho
authors.affiliation: Departamento de Genética e Biologia Evolutiva, Universidade de São Paulo, Rua do Matão, 277, Cidade Universitária, São Paulo, São Paulo 05508-090, Brazil.
authors.forename: G T
authors.initials: GT
authors.lastname: Silva
authors.affiliation: Departamento de Genética e Biologia Evolutiva, Universidade de São Paulo, Rua do Matão, 277, Cidade Universitária, São Paulo, São Paulo 05508-090, Brazil.
authors.forename: S S
authors.initials: SS
authors.lastname: Santos
authors.affiliation: Departamento de Genética e Biologia Evolutiva, Universidade de São Paulo, Rua do Matão, 277, Cidade Universitária, São Paulo, São Paulo 05508-090, Brazil.
authors.forename: L S
authors.initials: LS
authors.lastname: Miranda
authors.affiliation: Departamento de Genética e Biologia Evolutiva, Universidade de São Paulo, Rua do Matão, 277, Cidade Universitária, São Paulo, São Paulo 05508-090, Brazil.
authors.forename: L E
authors.initials: LE
authors.lastname: Araújo-Silva
authors.affiliation: Departamento de Genética e Biologia Evolutiva, Universidade de São Paulo, Rua do Matão, 277, Cidade Universitária, São Paulo, São Paulo 05508-090, Brazil.
authors.forename: R E
authors.initials: RE
authors.lastname: Ricklefs
authors.affiliation: Departamento de Genética e Biologia Evolutiva, Universidade de São Paulo, Rua do Matão, 277, Cidade Universitária, São Paulo, São Paulo 05508-090, Brazil.
authors.forename: C Y
authors.initials: CY
authors.lastname: Miyaki
authors.affiliation: Departamento de Genética e Biologia Evolutiva, Universidade de São Paulo, Rua do Matão, 277, Cidade Universitária, São Paulo, São Paulo 05508-090, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Maldonado-Coelho
mesh_terms: D000818:Animals; D001938:Brazil; D045303:Cytochromes b / Q000235:genetics; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D016787:Haemosporida / Q000302:isolation & purification*; D006321:Heart / Q000469:parasitology; D008099:Liver / Q000469:parasitology; D008289:Malaria, Avian / Q000097:blood / Q000469:parasitology*; D046109:Passeriformes / Q000097:blood / Q000469:parasitology*; D010369:Pectoralis Muscles / Q000469:parasitology; D010961:Plasmodium / Q000302:isolation & purification
chemical_list: D004272:DNA, Mitochondrial; D016054:DNA, Protozoan; D045303:Cytochromes b