title: The nuclear protein Sam68 is recruited to the cytoplasmic stress granules during enterovirus 71 infection.
abstract: Our previous study found that the nuclear protein, 68-kDa Src-associated in mitosis protein (Sam68), is translocated to the cytoplasm and forms punctate pattern during enterovirus 71 (EV71) infection [Virus Research, 180 (2014), 1-11]. However, the exact function of this punctate pattern in cytoplasm during EV71 infection remains unknown. In this study, we firstly have examined this punctate pattern of Sam68 re-localization in the cytoplasm, and observed the obvious recruitments of Sam68 to the EV71-induced stress granules (SGs). Sam68, belongs to the KH domain family of RNA binding proteins (RBPs), was then confirmed that its KH domain was essential for this recruitment. Nevertheless, Knockdown of Sam68 expression using ShRNA had no effects on SGs assembly, indicating that Sam68 is not a constitutive component of the SGs during EV71 infection. Lastly, we investigated the importance of microtubulin transport to SGs aggregation, and revealed that microtubule depolymerization inhibited SGs formation, suggesting that EV71-induced SGs move throughout the cytoplasm in a microtubule-dependent manner. Taken together, these results illuminated that EV71 infections can induce SGs formation, and Sam68, as a SGs component, migrates alone with SGs dependent on intact microtubule upon the viral infections. These findings may provide novel underlying mechanism for delineating the role of SGs during EV71 infection.
authors.affiliation: College of Animal Science and Veterinary Medicine, HeiLongJiang BaYi Agricultural University, Daqing 163319, China; College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Chen
authors.affiliation: Department of Nephrology, The Fifth Affiliated Hospital of Harbin Medical University, Daqing 163319, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Li
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China.
authors.forename: Ziye
authors.initials: Z
authors.lastname: Pan
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Ding
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China.
authors.forename: Dehua
authors.initials: D
authors.lastname: Zou
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China.
authors.forename: Liyang
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China.
authors.forename: Lijie
authors.initials: L
authors.lastname: Xiao
authors.affiliation: College of Animal Science and Veterinary Medicine, HeiLongJiang BaYi Agricultural University, Daqing 163319, China.
authors.forename: Binglei
authors.initials: B
authors.lastname: Shen
authors.affiliation: Daqing Branch of HeiLongJiang Academy of Sciences, Daqing 163319, China.
authors.forename: Shuxia
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Animal Science and Veterinary Medicine, HeiLongJiang BaYi Agricultural University, Daqing 163319, China; College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China. Electronic address: 549076831@qq.com.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Cao
authors.affiliation: College of Animal Science and Veterinary Medicine, HeiLongJiang BaYi Agricultural University, Daqing 163319, China; College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China. Electronic address: cuiyudong@yahoo.com.
authors.forename: Yudong
authors.initials: Y
authors.lastname: Cui
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000032:analysis*; D003594:Cytoplasmic Granules / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000032:analysis*; D029821:Enterovirus A, Human / Q000254:growth & development*; D006367:HeLa Cells; D006801:Humans; D021381:Protein Transport; D016601:RNA-Binding Proteins / Q000032:analysis*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D004268:DNA-Binding Proteins; C489559:KHDRBS1 protein, human; D016601:RNA-Binding Proteins
keywords: Enterovirus 71; RNA-binding proteins; Sam68; Stress granules