title: Comparative Analysis of Begonia Plastid Genomes and Their Utility for Species-Level Phylogenetics.
abstract: Recent, rapid radiations make species-level phylogenetics difficult to resolve. We used a multiplexed, high-throughput sequencing approach to identify informative genomic regions to resolve phylogenetic relationships at low taxonomic levels in Begonia from a survey of sixteen species. A long-range PCR method was used to generate draft plastid genomes to provide a strong phylogenetic backbone, identify fast evolving regions and provide informative molecular markers for species-level phylogenetic studies in Begonia.
authors.affiliation: NIAB EMR, East Malling, Kent, United Kingdom.|The University of Reading, Whiteknights, Reading, Berkshire, United Kingdom.
authors.forename: Nicola
authors.initials: N
authors.lastname: Harrison
authors.affiliation: NIAB EMR, East Malling, Kent, United Kingdom.|The University of Reading, Whiteknights, Reading, Berkshire, United Kingdom.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Harrison
authors.affiliation: Royal Botanic Gardens Edinburgh, Edinburgh, Scotland, United Kingdom.|The University of Edinburgh, Darwin Building, King's Buildings, Edinburgh, Scotland, United Kingdom.
authors.forename: Catherine A
authors.identifier: http://orcid.org/0000-0001-6426-3000
authors.initials: CA
authors.lastname: Kidner
mesh_terms: D001482:Base Composition; D001499:Bayes Theorem; D031112:Begoniaceae / Q000145:classification* / Q000235:genetics*; D003062:Codon; D018553:Cucumis sativus / Q000145:classification / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D019143:Evolution, Molecular; D005819:Genetic Markers; D018745:Genome, Plant; D054627:Genome, Plastid; D016013:Likelihood Functions; D008957:Models, Genetic; D058977:Molecular Sequence Annotation; D010802:Phylogeny; D018087:Plastids / Q000235:genetics; D013045:Species Specificity
chemical_list: D003062:Codon; D018744:DNA, Plant; D005819:Genetic Markers