title: Production of biomass and lipids by the oleaginous microalgae Monoraphidium sp. QLY-1 through heterotrophic cultivation and photo-chemical modulator induction.
abstract: A two-step strategy comprising heterotrophic cultivation and photo-chemical modulator induction was developed to enhance biomass and lipid accumulation in the oleaginous Monoraphidium sp. QLY-1, which was isolated from Qilu Lake in Yunnan Plateau. The algae were first cultivated heterotrophically to achieve high biomass concentration (5.54gL(-1)) with a lipid content of 22.47%. The cultivated algae were diluted, transferred to light environment, and treated with different chemical elicitors. Results showed that the lipid content increased to 36.68% after 3-day of photoinduction. The lipid content was further enhanced by 1.21, 1.32, and 1.29 folds in algal cells treated with nitrogen deficiency, 20gL(-1) NaCl, and 5mM glycine betaine, respectively. The maximum lipid content (48.54%) and lipid productivity (121.27mgL(-1)d(-1)) were obtained in treatments with 20gL(-1) NaCl and 5mM GB, respectively. This study proposes a strategy to efficiently produce lipids by using microalgae.
authors.affiliation: Faculty of Life Sciences and Technology, Kunming University of Science and Technology, Kunming 650500, China.
authors.forename: Yongteng
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Faculty of Life Sciences and Technology, Kunming University of Science and Technology, Kunming 650500, China.
authors.forename: Dafei
authors.initials: D
authors.lastname: Li
authors.affiliation: Faculty of Life Sciences and Technology, Kunming University of Science and Technology, Kunming 650500, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Ding
authors.affiliation: Faculty of Life Sciences and Technology, Kunming University of Science and Technology, Kunming 650500, China.
authors.forename: Raoqiong
authors.initials: R
authors.lastname: Che
authors.affiliation: Faculty of Life Sciences and Technology, Kunming University of Science and Technology, Kunming 650500, China.
authors.forename: Jun-Wei
authors.initials: JW
authors.lastname: Xu
authors.affiliation: Faculty of Life Sciences and Technology, Kunming University of Science and Technology, Kunming 650500, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Faculty of Life Sciences and Technology, Kunming University of Science and Technology, Kunming 650500, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: School of Foreign Languages, Kunming University, Kunming 650200, China.
authors.forename: Huixian
authors.initials: H
authors.lastname: Ma
authors.affiliation: Faculty of Life Sciences and Technology, Kunming University of Science and Technology, Kunming 650500, China. Electronic address: xuya_yu@163.com.
authors.forename: Xuya
authors.initials: X
authors.lastname: Yu
mesh_terms: D001622:Betaine / Q000494:pharmacology; D018533:Biomass; D000460:Chlorophyta / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D052836:Heterotrophic Processes / Q000187:drug effects; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism*; D055668:Photochemical Processes / Q000187:drug effects; D052817:Phototrophic Processes / Q000187:drug effects; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D005227:Fatty Acids; D008055:Lipids; D012337:RNA, Ribosomal, 18S; D017382:Reactive Oxygen Species; D001622:Betaine; D012965:Sodium Chloride
keywords: Glycine betaine; Lipid induction; Monoraphidium sp. QLY-1; NaCl; Two-step cultivation