title: Fabrication of DNA/RNA Hybrids Through Sequence-Specific Scission of Both Strands by pcPNA-S1 Nuclease Combination.
abstract: By combining two strands of pseudo-complementary peptide nucleic acid (pcPNA) with S1 nuclease, a tool for site-selective and dual-strand scission of DNA/RNA hybrids has been developed. Both of the DNA and the RNA strands in the hybrids are hydrolyzed at desired sites to provide unique sticky ends. The scission fragments are directly ligated with other DNA/RNA hybrids by using T4 DNA ligase, resulting in the formation of desired recombinant DNA/RNA hybrids.
authors.affiliation: a University of Tsukuba , Tsukuba , Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Futai
authors.affiliation: a University of Tsukuba , Tsukuba , Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Sumaoka
authors.affiliation: a University of Tsukuba , Tsukuba , Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Komiyama
mesh_terms: D001483:Base Sequence; D053837:DNA Cleavage; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics; D006801:Humans; D020014:K562 Cells; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D012313:RNA / Q000737:chemistry / Q000235:genetics; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000737:chemistry*
chemical_list: D004277:DNA, Single-Stranded; D020135:Peptide Nucleic Acids; C039919:RNA, recombinant; D012313:RNA; D015719:Single-Strand Specific DNA and RNA Endonucleases
keywords: DNA/RNA hybrids; S1 nuclease; genetic engineering; pcPNA; recombination; sequence-specific scission