title: New partners and phosphorylation sites of focal adhesion kinase identified by mass spectrometry.
abstract: The regulation of focal adhesion kinase (FAK) involves phosphorylation and multiple interactions with other signaling proteins. Some of these pathways are relevant for nervous system functions such as branching, axonal guidance, and plasticity. In this study, we screened mouse brain to identify FAK-interactive proteins and phosphorylatable residues as a first step to address the neuronal functions of this kinase. Using mass spectrometry analysis, we identified new phosphorylated sites (Thr 952, Thr 1048, and Ser 1049), which lie in the FAT domain; and putative new partners for FAK, which include cytoskeletal proteins such as drebrin and MAP 6, adhesion regulators such as neurabin-2 and plakophilin 1, and synapse-associated proteins such as SynGAP and a NMDA receptor subunit. Our findings support the participation of brain-localized FAK in neuronal plasticity.
authors.affiliation: Developmental Neurobiology and Neural Regeneration Group, Department of Cell Biology, Faculty of Biology, University of Barcelona, Diagonal 643, 08038 Barcelona, Spain; Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), ISCIII, 28031 Madrid, Spain.
authors.forename: Maria del Mar
authors.initials: Mdel M
authors.lastname: Masdeu
authors.affiliation: Developmental Neurobiology and Neural Regeneration Group, Department of Cell Biology, Faculty of Biology, University of Barcelona, Diagonal 643, 08038 Barcelona, Spain; Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), ISCIII, 28031 Madrid, Spain.
authors.forename: Beatriz G
authors.initials: BG
authors.lastname: Armendáriz
authors.affiliation: Developmental Neurobiology and Neural Regeneration Group, Department of Cell Biology, Faculty of Biology, University of Barcelona, Diagonal 643, 08038 Barcelona, Spain; Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), ISCIII, 28031 Madrid, Spain; Vall d´Hebron Institute of Research, 08035 Barcelona, Spain; Institució Catalana de Recerca i Estudis Avançats (ICREA), 08010 Barcelona, Spain.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Soriano
authors.affiliation: Developmental Neurobiology and Neural Regeneration Group, Department of Cell Biology, Faculty of Biology, University of Barcelona, Diagonal 643, 08038 Barcelona, Spain; Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), ISCIII, 28031 Madrid, Spain.
authors.forename: Jesús Mariano
authors.initials: JM
authors.lastname: Ureña
authors.affiliation: Developmental Neurobiology and Neural Regeneration Group, Department of Cell Biology, Faculty of Biology, University of Barcelona, Diagonal 643, 08038 Barcelona, Spain; Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), ISCIII, 28031 Madrid, Spain. Electronic address: fburgaya@ub.edu.
authors.forename: Ferran
authors.initials: F
authors.lastname: Burgaya
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001665:Binding Sites; D001921:Brain / Q000201:enzymology* / Q000503:physiopathology; D020134:Catalytic Domain; D002853:Chromatography, Liquid; D004195:Disease Models, Animal; D004789:Enzyme Activation; D051417:Focal Adhesion Kinase 1 / Q000737:chemistry / Q000378:metabolism*; D047468:Immunoprecipitation; D051379:Mice; D009473:Neuronal Plasticity; D010433:Pentylenetetrazole; D010766:Phosphorylation; D011485:Protein Binding; D012640:Seizures / Q000201:enzymology* / Q000503:physiopathology; D015398:Signal Transduction; D053719:Tandem Mass Spectrometry
chemical_list: D051417:Focal Adhesion Kinase 1; C496051:Ptk2 protein, mouse; D010433:Pentylenetetrazole
keywords: Brain; Focal adhesion kinase; Mass spectrometry; Synapse