title: Effect of heat shock protein 70 polymorphism on thermotolerance in Tharparkar cattle.
abstract: OBJECTIVE
  Out of various members of heat shock protein (HSP) superfamily which act a molecular chaperon by binding to the denaturing protein thus stabilizing them and preserving their activity, HSP70 are of major importance in thermotolerance development. Thus, present investigation aimed at a screening of HSP70 gene for polymorphisms and possible differences in thermotolerance in Tharparkar breed of cattle.
  
  
  METHODS
  A 295 bp fragment of HSP70 gene was subjected to polymerase chain reaction-single-strand conformation polymorphism (SSCP) followed by sequencing of different SSCP patterns in 64 Tharparkar cattle. A comparative thermotolerance of identified genotypes was analyzed using heat tolerance coefficients (HTCs) of animals for different seasons.
  
  
  RESULTS
  Three SSCP patterns and consequently two alleles namely A and B were documented in one fragment of HSP70 gene. On sequencing, one single-nucleotide polymorphism with G > T substitution was found at a position that led to a change of amino acid aspartate to tyrosine in allele A. It was found that in maintaining near normal average rectal temperature, genotype AA was superior (p≤0.01). Genotype AA, thus, was found to be most thermotolerant genotype with the highest HTC (p≤0.01).
  
  
  CONCLUSIONS
  The polymorphism at HSP70 is expected to be a potent determinant for heat tolerance in cattle, which may aid in selection for thermotolerance in cattle.
authors.affiliation: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.
authors.forename: Sandip
authors.initials: S
authors.lastname: Bhat
authors.affiliation: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.
authors.forename: Pushpendra
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.
authors.forename: Neeraj
authors.initials: N
authors.lastname: Kashyap
authors.affiliation: Department of Animal Genetics and Breeding, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India.
authors.forename: Bharti
authors.initials: B
authors.lastname: Deshmukh
authors.affiliation: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.
authors.forename: Mahesh Shivanand
authors.initials: MS
authors.lastname: Dige
authors.affiliation: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.
authors.forename: Bharat
authors.initials: B
authors.lastname: Bhushan
authors.affiliation: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.
authors.forename: Anuj
authors.initials: A
authors.lastname: Chauhan
authors.affiliation: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.
authors.forename: Gyanendra
authors.initials: G
authors.lastname: Singh
keywords: cattle; heat shock protein 70; heat tolerance; polymorphism, Tharparkar