title: Exercise amelioration of depression-like behavior in OVX mice is associated with suppression of NLRP3 inflammasome activation in hippocampus.
abstract: Exercise has benefit for perimenopause women in many ways, such as affective disorders. Our previous study has demonstrated that inflammation in hippocampus contributes to development of depression-like behavior in ovariectomized (OVX) rats. Recently, oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome has been implicated to be involved in lipopolysaccharide (LPS)- and chronic stress-induced depression-like behavior in rodents. We sought to investigate whether ovariectomy-induced depression-like behavior is associated with NLRP3 inflammasome activation in brain and the effect of exercise on NLRP3 inflammasome activation in this model. The results showed that ovariectomy resulted in depression-like behavior in mice and an increase in levels of IL-1β and IL-18 in hippocampus. Exercise ameliorated the depression-like behavior and decreased levels of IL-1β and IL-18 in hippocampus. The level of IL-1β and IL-18 in hippocampus correlated to depression-like behavior in OVX mice. The levels of NLRP3, cleaved caspase-1 P10 and CD11b in hippocampus were increased in OVX mice compared with control group. Exercise could reduce the levels of NLRP3, cleaved caspase-1 P10 and CD11b in OVX mice. Our study suggests that NLRP3 inflammasome activation contribute to inflammation in hippocampus upon to deprivation of ovary. Exercise amelioration of depression-like behavior is associated with suppression of NLRP3 inflammasome activation in hippocampus of this model.
authors.affiliation: School of Kinesiology, The Key Laboratory of Exercise and Health Sciences of Ministry of Education, Shanghai University of Sport, Shanghai 200438, China.
authors.forename: Yujun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Physiology, Second Military Medical University, Shanghai, China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Physiology, Second Military Medical University, Shanghai, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Sheng
authors.affiliation: Department of Physiology, Second Military Medical University, Shanghai, China. Electronic address: nixin@smmu.edu.cn.
authors.forename: Xin
authors.initials: X
authors.lastname: Ni
authors.affiliation: School of Kinesiology, The Key Laboratory of Exercise and Health Sciences of Ministry of Education, Shanghai University of Sport, Shanghai 200438, China. Electronic address: lujianqiang@sus.edu.cn.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Lu
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism; D003863:Depression / Q000209:etiology / Q000473:pathology / Q000534:rehabilitation; D004195:Disease Models, Animal; D004660:Encephalitis / Q000150:complications / Q000209:etiology / Q000473:pathology; D004958:Estradiol / Q000097:blood; D005081:Exercise Therapy / Q000379:methods*; D005106:Exploratory Behavior; D005260:Female; D005518:Food Preferences / Q000523:psychology; D005786:Gene Expression Regulation / Q000502:physiology; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D058847:Inflammasomes / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000633:toxicity; D051379:Mice; D008810:Mice, Inbred C57BL; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein / Q000235:genetics / Q000378:metabolism*; D010052:Ovariectomy / Q000009:adverse effects*; D013395:Sucrose / Q000008:administration & dosage; D013550:Swimming / Q000523:psychology
chemical_list: D016207:Cytokines; D058847:Inflammasomes; D008070:Lipopolysaccharides; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C492716:Nlrp3 protein, mouse; D004958:Estradiol; D013395:Sucrose
keywords: Depression; Estrogen; Exercise; NLRP3 inflammasome