title: Oryza sativa H+-ATPase (OSA) is Involved in the Regulation of Dumbbell-Shaped Guard Cells of Rice.
abstract: The stomatal apparatus consists of a pair of guard cells and regulates gas exchange between the leaf and atmosphere. In guard cells, blue light (BL) activates H(+)-ATPase in the plasma membrane through the phosphorylation of its penultimate threonine, mediating stomatal opening. Although this regulation is thought to be widely adopted among kidney-shaped guard cells in dicots, the molecular basis underlying that of dumbbell-shaped guard cells in monocots remains unclear. Here, we show that H(+)-ATPases are involved in the regulation of dumbbell-shaped guard cells. Stomatal opening of rice was promoted by the H(+)-ATPase activator fusicoccin and by BL, and the latter was suppressed by the H(+)-ATPase inhibitor vanadate. Using H(+)-ATPase antibodies, we showed the presence of phosphoregulation of the penultimate threonine in Oryza sativa H(+)-ATPases (OSAs) and localization of OSAs in the plasma membrane of guard cells. Interestingly, we identified one H(+)-ATPase isoform, OSA7, that is preferentially expressed among the OSA genes in guard cells, and found that loss of function of OSA7 resulted in partial insensitivity to BL. We conclude that H(+)-ATPase is involved in BL-induced stomatal opening of dumbbell-shaped guard cells in monocotyledon species.
authors.affiliation: Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, 464-8602 Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Toda
authors.affiliation: Institute for Advanced Research, Nagoya University, Chikusa, Nagoya, 464-8602 Japan.
authors.forename: Yin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences, Tsukuba, 305-8602 Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Takahashi
authors.affiliation: Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, 464-8602 Japan.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Kawai
authors.affiliation: Center of Gene Research, Nagoya University, Chikusa, Nagoya, 464-8602 Japan.
authors.forename: Yasuomi
authors.initials: Y
authors.lastname: Tada
authors.affiliation: Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki, 710-0046 Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamaji
authors.affiliation: Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki, 710-0046 Japan.
authors.forename: Jian
authors.initials: J
authors.lastname: Feng Ma
authors.affiliation: Bioscience Center, Nagoya University, Chikusa, Nagoya, 464-8601 Japan.
authors.forename: Motoyuki
authors.initials: M
authors.lastname: Ashikari
authors.affiliation: Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, 464-8602 Japan Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Chikusa, Nagoya, 464-8602 Japan kinoshita@bio.nagoya-u.ac.jp.
authors.forename: Toshinori
authors.initials: T
authors.lastname: Kinoshita
mesh_terms: D000595:Amino Acid Sequence; D048430:Cell Shape / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D008027:Light; D012275:Oryza / Q000166:cytology* / Q000201:enzymology* / Q000235:genetics / Q000528:radiation effects; D010766:Phosphorylation / Q000528:radiation effects; D010769:Phosphothreonine / Q000378:metabolism; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D054046:Plant Stomata / Q000166:cytology* / Q000502:physiology / Q000528:radiation effects; D006180:Proton-Translocating ATPases / Q000737:chemistry / Q000378:metabolism*; D036226:Seedlings / Q000378:metabolism / Q000528:radiation effects
chemical_list: D010940:Plant Proteins; D010769:Phosphothreonine; D006180:Proton-Translocating ATPases
keywords: Dumbbell-type stomata; H+-ATPase; Light-induced stomatal opening; Rice