title: Identification of Dermatophilus congolensis from lower leg dermatitis of cattle in Kerala, India.
abstract: This study was conducted to identify the aetiological agents associated with a particular type of lower leg dermatitis, locally called pododermatitis, among dairy cattle in Kerala. Skin scabs and scrapings were collected aseptically from 82 naturally occurring cases of lower leg dermatitis in cattle and were subjected to direct microscopical examination and bacterial and fungal culture. Microscopical examination of the skin scrapings with 10% potassium hydroxide revealed fungal spores in hair shafts from only two samples and did not reveal the presence of mites or other parasites. Fungal culture yielded dermatophytes from only five samples; these were identified as Trichophyton mentagrophytes in two cases, T verrucosum in one case, Epidermophyton floccosum in one case and Microsporum nanum in one case. Microscopical examination of Giemsa- and Gram-stained smears of the scab material from the lesions from 72 cases revealed characteristic Gram-positive septate branching filaments with multiple rows of spherical to ovoid cocci, with a typical 'tram-track' appearance suggestive of Dermatophilus congolensis. Culture of the scab materials on sheep blood agar in the presence of 10% carbon dioxide yielded typical beta haemolytic colonies of D. congolensis from 75 samples. The isolates were further confirmed by the macroscopic and microscopic morphology of the colonies, and biochemical test results. This study confirmed the presence of dermatophilosis caused by D. congolensis in cattle in Kerala.
authors.forename: P V
authors.initials: PV
authors.lastname: Tresamol
authors.forename: M R
authors.initials: MR
authors.lastname: Saseendranath
authors.forename: H
authors.initials: H
authors.lastname: Subramanian
authors.forename: U N
authors.initials: UN
authors.lastname: Pillai
authors.forename: M
authors.initials: M
authors.lastname: Mini
authors.forename: S
authors.initials: S
authors.lastname: Ajithkumar
mesh_terms: D039903:Actinobacteria / Q000302:isolation & purification*; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology*; D005121:Extremities / Q000382:microbiology* / Q000473:pathology; D007194:India; D017192:Skin Diseases, Bacterial / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*