title: Recent advances in yeast cell-surface display technologies for waste biorefineries.
abstract: Waste biorefinery aims to maximize the output of value-added products from various artificial/agricultural wastes by using integrated bioprocesses. To make waste biorefinery economically feasible, it is thus necessary to develop a low-cost, environment-friendly technique to perform simultaneous biodegradation and bioconversion of waste materials. Cell-surface display engineering is a novel, cost-effective technique that can auto-immobilize proteins on the cell exterior of microorganisms, and has been applied for use with waste biofinery. Through tethering different enzymes (e.g., cellulase, lipase, and protease) or metal-binding peptides on cell surfaces, various yeast strains can effectively produce biofuels and biochemicals from sugar/protein-rich waste materials, catalyze waste oils into biodiesels, or retrieve heavy metals from wastewater. This review critically summarizes recent applications of yeast cell-surface display on various types of waste biorefineries, highlighting its potential and future challenges with regard to commercializing this technology.
authors.affiliation: Department of Chemical Science and Engineering, Kobe University, Kobe, Japan.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute Technology, Harbin, PR China. Electronic address: stephen6949@hit.edu.cn.
authors.forename: Shih-Hsin
authors.initials: SH
authors.lastname: Ho
authors.affiliation: Organization of Advanced Science and Technology, Kobe University, Kobe, Japan.
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Hasunuma
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Taiwan; Research Center for Energy Technology and Strategy, National Cheng Kung University, Taiwan.
authors.forename: Jo-Shu
authors.initials: JS
authors.lastname: Chang
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute Technology, Harbin, PR China.
authors.forename: Nan-Qi
authors.initials: NQ
authors.lastname: Ren
authors.affiliation: Department of Chemical Science and Engineering, Kobe University, Kobe, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D001673:Biodegradation, Environmental; D056804:Biofuels / Q000382:microbiology*; D006801:Humans; D019216:Metals, Heavy / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000502:physiology; D018505:Waste Management / Q000379:methods*; D062065:Wastewater / Q000737:chemistry / Q000382:microbiology; D015003:Yeasts / Q000166:cytology / Q000502:physiology*
chemical_list: D056804:Biofuels; D019216:Metals, Heavy; D062065:Wastewater
keywords: Biochemical; Biofuel; Cell-surface display engineering; Heavy metal; Waste biorefinery; Yeast