title: An extremely alkaline mannanase from Streptomyces sp. CS428 hydrolyzes galactomannan producing series of mannooligosaccharides.
abstract: An alkaline-thermostable mannanase from Streptomyces sp. CS428 was produced, purified, and biochemically characterized. The extracellular mannanase (Mn428) was purified to homogeneity with 12.4 fold, specific activity of 2406.7 U/mg, and final recovery of 37.6 %. The purified β-mannanase was found to be a monomeric protein with a molecular mass of approximately 35 kDa as analyzed by SDS-PAGE and zymography. The first N-terminal amino acid sequences of mannanase enzyme were HIRNGNHQLPTG. The optimal temperature and pH for enzyme were 60 °C and 12.5, respectively. The mannanase activities were significantly affected by the presence of metal ions, modulators, and detergents. Km and Vmax values of Mn428 were 1.01 ± 3.4 mg/mL and 5029 ± 85 µmol/min mg, respectively when different concentrations (0.6-10 mg/mL) of locust bean gum galactomannan were used as substrate. The substrate specificity of enzyme showed its highest specificity towards galactomannan which was further hydrolyzed to produce mannose, mannobiose, mannotriose, and a series of mannooligosaccharides. Mannooligosaccharides can be further converted to ethanol production, thus the purified β-mannanase isolated from Streptomyces sp. CS428 was found to be attractive for biotechnological applications.
authors.affiliation: Department of Pharmacy, College of Pharmacy, Chosun University, Gwangju, 501-759, Korea.
authors.lastname: Pradeep G C
authors.affiliation: Department of Pharmacy, Mokpo National University, Muan, Jeonnam, 534-729, Korea.
authors.forename: Seung Sik
authors.initials: SS
authors.lastname: Cho
authors.affiliation: Department of Pharmacy, College of Pharmacy, Chosun University, Gwangju, 501-759, Korea.
authors.forename: Yun Hee
authors.initials: YH
authors.lastname: Choi
authors.affiliation: Department of Pharmacy, College of Pharmacy, Chosun University, Gwangju, 501-759, Korea.
authors.forename: Yun Seok
authors.initials: YS
authors.lastname: Choi
authors.affiliation: Department of Pharmacy, College of Pharmacy, Chosun University, Gwangju, 501-759, Korea.
authors.forename: Jun-Pil
authors.initials: JP
authors.lastname: Jee
authors.affiliation: Department of Biology, Sunchon National University, Sunchon, Chonnam, 540-742, Korea.
authors.forename: Chi Nam
authors.initials: CN
authors.lastname: Seong
authors.affiliation: Department of Pharmacy, College of Pharmacy, Chosun University, Gwangju, 501-759, Korea. jcyu@chosun.ac.kr.
authors.forename: Jin Cheol
authors.initials: JC
authors.lastname: Yoo
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D055162:Biocatalysis; D004795:Enzyme Stability; D005690:Galactose / Q000031:analogs & derivatives; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008351:Mannans / Q000737:chemistry* / Q000378:metabolism; D008970:Molecular Weight; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism; D013302:Streptomyces / Q000737:chemistry / Q000201:enzymology*; D013379:Substrate Specificity; D044902:beta-Mannosidase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D008351:Mannans; D009844:Oligosaccharides; C012990:galactomannan; C014647:mannobiose; D044902:beta-Mannosidase; D005690:Galactose
keywords: Alkali-thermostable; Mannooligosaccharides; β-Mannanase