title: Study of photodynamic activity of Au@SiO2 core-shell nanoparticles in vitro.
abstract: Metal-semiconductor core-shell type Au@SiO2 nanoparticles were prepared by Stober's method. They were characterized by absorption, XRD, HR-TEM and EDAX techniques. The resulting modified core-shell nanoparticles shows that the formation of singlet oxygen, which was confirmed by ESR technique. The photohemolysis studies were carried out under two different experimental conditions. It is observed that the photohemolysis increases with concentration as well as light dose. Cell viability of the core-shell nanoparticles against HeLa cell lines were studied by MTT assay method. The outcomes of the present study indicate that, the Au@SiO2 core-shell nanoparticles are extremely stable with a very high photodynamic efficiency under visible light illumination.
authors.affiliation: PG & Research Department of Chemistry, Queen Mary's College, Chennai 600 004, Tamil Nadu, India. Electronic address: dhanamveni88@gmail.com.
authors.forename: K S
authors.initials: KS
authors.lastname: Meena
authors.affiliation: PG & Research Department of Chemistry, Queen Mary's College, Chennai 600 004, Tamil Nadu, India. Electronic address: dhanalekshmi6@gmail.com.
authors.forename: K I
authors.initials: KI
authors.lastname: Dhanalekshmi
authors.affiliation: Department of Chemistry, St. Joseph's College of Engineering, Chennai 600119, Tamil Nadu, India.
authors.forename: K
authors.initials: K
authors.lastname: Jayamoorthy
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D004578:Electron Spin Resonance Spectroscopy; D004912:Erythrocytes / Q000166:cytology; D016166:Free Radical Scavengers / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006461:Hemolysis / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D008027:Light; D046529:Microscopy, Electron, Transmission; D045663:Quantum Dots / Q000737:chemistry* / Q000633:toxicity; D017382:Reactive Oxygen Species / Q000378:metabolism; D012822:Silicon Dioxide / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D006046:Gold; D012822:Silicon Dioxide
keywords: Cell viability; Core-shell nanoparticles; MTT assay; Photodynamic activity; Photohemolysis; Reactive oxygen species