title: mRNA and protein dataset of autophagy markers (LC3 and p62) in several cell lines.
abstract: We characterized the dynamics of autophagy in vitro using four different cell systems and analyzing markers widely used in this field, i.e. LC3 (microtubule-associated protein 1 light chain 3; protein recruited from the cytosol (LC3-I) to the autophagosomal membrane where it is lipidated (LC3-II)) and p62/SQSTM1 (adaptor protein that serves as a link between LC3 and ubiquitinated substrates), (Klionsky et al., 2016) [1]. Data provided include analyses of protein levels of LC3 and p62 by Western-blotting and endogenous immunofluorescence experiments, but also p62 mRNA levels obtained by quantitative PCR (qPCR). To monitor the turnover of these autophagy markers and, thus, measure the flux of this pathway, cells were under starvation conditions and/or treated with bafilomycin A1 (Baf. A1) to block fusion of autophagosomes with lysosomes.
authors.affiliation: Centro de Investigación Biomédica en Red en Enfermedades Neurodegenerativas (CIBERNED), Spain; Department of Cell Biology, University of Groningen, University Medical Center Groningen, A. Deusinglaan 1, 9713 AV Groningen, The Netherlands.
authors.forename: Rubén
authors.initials: R
authors.lastname: Gómez-Sánchez
authors.affiliation: Centro de Investigación Biomédica en Red en Enfermedades Neurodegenerativas (CIBERNED), Spain; Departamento de Bioquímica, Biología Molecular y Genética. Facultad de Enfermería y Terapia Ocupacional. Avda de la Universidad S/N C.P, 10003 Cáceres, Spain.
authors.forename: Sokhna M S
authors.initials: SM
authors.lastname: Yakhine-Diop
authors.affiliation: Centro de Investigación Biomédica en Red en Enfermedades Neurodegenerativas (CIBERNED), Spain; Departamento de Bioquímica, Biología Molecular y Genética. Facultad de Enfermería y Terapia Ocupacional. Avda de la Universidad S/N C.P, 10003 Cáceres, Spain.
authors.forename: Mario
authors.initials: M
authors.lastname: Rodríguez-Arribas
authors.affiliation: Centro de Investigación Biomédica en Red en Enfermedades Neurodegenerativas (CIBERNED), Spain; Equipe 11 labellisée Ligue contre le Cancer, Centre de Recherche des Cordeliers, 75006 Paris, France; INSERM U1138, 75006 Paris, France; Université Paris Descartes/Paris V, Sorbonne Paris Cité, 75006 Paris, France; Université Pierre et Marie Curie/Paris VI, 75006 Paris, France; Gustave Roussy Comprehensive Cancer Institute, 94805 Villejuif, France.
authors.forename: José M
authors.initials: JM
authors.lastname: Bravo-San Pedro
authors.affiliation: Centro de Investigación Biomédica en Red en Enfermedades Neurodegenerativas (CIBERNED), Spain; Departamento de Bioquímica, Biología Molecular y Genética. Facultad de Enfermería y Terapia Ocupacional. Avda de la Universidad S/N C.P, 10003 Cáceres, Spain.
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Martínez-Chacón
authors.affiliation: Centro de Investigación Biomédica en Red en Enfermedades Neurodegenerativas (CIBERNED), Spain; Departamento de Bioquímica, Biología Molecular y Genética. Facultad de Enfermería y Terapia Ocupacional. Avda de la Universidad S/N C.P, 10003 Cáceres, Spain.
authors.forename: Elisabet
authors.initials: E
authors.lastname: Uribe-Carretero
authors.affiliation: Centro de Investigación Biomédica en Red en Enfermedades Neurodegenerativas (CIBERNED), Spain; Departamento de Bioquímica, Biología Molecular y Genética. Facultad de Enfermería y Terapia Ocupacional. Avda de la Universidad S/N C.P, 10003 Cáceres, Spain.
authors.forename: Diana C J
authors.initials: DC
authors.lastname: Pinheiro de Castro
authors.affiliation: Centro de Investigación Biomédica en Red en Enfermedades Neurodegenerativas (CIBERNED), Spain; Departamento de Bioquímica, Biología Molecular y Genética. Facultad de Enfermería y Terapia Ocupacional. Avda de la Universidad S/N C.P, 10003 Cáceres, Spain.
authors.forename: Elisa
authors.initials: E
authors.lastname: Pizarro-Estrella
authors.affiliation: Centro de Investigación Biomédica en Red en Enfermedades Neurodegenerativas (CIBERNED), Spain; Departamento de Bioquímica, Biología Molecular y Genética. Facultad de Enfermería y Terapia Ocupacional. Avda de la Universidad S/N C.P, 10003 Cáceres, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Fuentes
authors.affiliation: Centro de Investigación Biomédica en Red en Enfermedades Neurodegenerativas (CIBERNED), Spain; Departamento de Bioquímica, Biología Molecular y Genética. Facultad de Enfermería y Terapia Ocupacional. Avda de la Universidad S/N C.P, 10003 Cáceres, Spain.
authors.forename: Rosa A
authors.initials: RA
authors.lastname: González-Polo
keywords: Autophagy; Baf. A1, Bafilomycin A1; EBSS, Earle׳s Balanced Salt Solution; FBS, Fetal bovine serum; GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; HFs, Human fibroblasts; LC3; LC3, Microtubule-associated protein 1 light chain 3; MEFs, Mouse embryonic fibroblasts; PBS, Phosphate-buffered saline; SB, Sample buffer; SDS, Sodium dodecyl sulfate; TBST, Tris-buffered saline with Tween 20; Western-blot; p62; qPCR, Quantitative PCR