title: Effects of Jia-Wei-Xiao-Yao-San on the Peripheral and Lymphatic Pharmacokinetics of Paclitaxel in Rats.
abstract: Paclitaxel is effective against breast cancer. The herbal medicine, Jia-Wei-Xiao-Yao-San (JWXYS), is the most frequent prescription used to relieve the symptoms of breast cancer treatments. The aim of the study was to investigate the herb-drug interaction effects of a herbal medicine on the distribution of paclitaxel to lymph. A validated ultraperformance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method was used to determine the paclitaxel levels in rat plasma and lymph after intravenous infusion of paclitaxel alone with or without 7 days of JWXYS pretreatment. The pharmacokinetic results indicate that paclitaxel concentrations in plasma exceeded those in lymph by approximately 3.6-fold. The biodistribution of paclitaxel from plasma to lymph was 39 ± 5%; however, this increased to 45 ± 4% with JWXYS pretreatment. With JWXYS pretreatment, the AUC and C max of paclitaxel in plasma were significantly reduced by approximately 1.5-fold, compared to paclitaxel alone. Additionally, JWXYS decreased the AUC and C max of paclitaxel in lymph. However, the lymph absorption rate of paclitaxel with or without JWXYS pretreatment was not significantly changed (27 ± 3 and 30 ± 2%, resp.). Our findings demonstrate that when paclitaxel is prescribed concurrently with herbal medicine, monitoring of the blood pharmacokinetics of paclitaxel is recommended.
authors.affiliation: Institute of Traditional Medicine, National Yang-Ming University, No. 155, Section 2, Li-Nong Street, Taipei 112, Taiwan.
authors.forename: Mei-Ling
authors.initials: ML
authors.lastname: Hou
authors.affiliation: Institute of Traditional Medicine, National Yang-Ming University, No. 155, Section 2, Li-Nong Street, Taipei 112, Taiwan.
authors.forename: Chia-Ming
authors.initials: CM
authors.lastname: Lu
authors.affiliation: Institute of Traditional Medicine, National Yang-Ming University, No. 155, Section 2, Li-Nong Street, Taipei 112, Taiwan; Graduate Institute of Acupuncture Science, China Medical University, No. 91, Hsueh-Shih Road, Taichung 404, Taiwan; School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung 807, Taiwan; Department of Education and Research, Taipei City Hospital, No. 145, Zhengzhou Road, Datong District, Taipei 103, Taiwan.
authors.forename: Tung-Hu
authors.identifier: 0000-0002-9007-2547
authors.initials: TH
authors.lastname: Tsai