title: Soybean C2H2-Type Zinc Finger Protein GmZFP3 with Conserved QALGGH Motif Negatively Regulates Drought Responses in Transgenic Arabidopsis.
abstract: Plant response to environmental stresses is regulated by a complicated network of regulatory and functional genes. In this study, we isolated the putative stress-associated gene GmZFP3 (a C2H2-type Zinc finger protein gene) based on the previous finding that it was one of two genes located in the QTL region between the Satt590 and Satt567 markers related to soybean tolerance to drought. Temporal and spatial expression analysis using quantitative real-time PCR indicated that GmZFP3 was primarily expressed in roots, stems and leaf organs and was expressed at low levels in flowers and soybean pods. Moreover, GmZFP3 expression increased in response to polyethylene glycol (PEG) and Abscisic acid (ABA) treatments. In addition, subcellular localization analysis indicated that GmZFP3 was ubiquitously distributed in plant cells. Transgenic experiments indicated that GmZFP3 played a negative role in plant tolerance to drought. Analysis of ABA-related marker gene expression in Arabidopsis suggested that GmZFP3 might be involved in the ABA-dependent pathway during the drought stress response. Taken together, these results suggest that soybean GmZFP3 negatively regulates the drought response.
authors.affiliation: Jiangsu Key Laboratory for Bioresources of Saline Soils, Provincial Key Laboratory of Agrobiology, Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences Nanjing, China.
authors.forename: Dayong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Institute of Botany, Chinese Academy of Sciences Nanjing, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Tong
authors.affiliation: Jiangsu Key Laboratory for Bioresources of Saline Soils, Provincial Key Laboratory of Agrobiology, Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences Nanjing, China.
authors.forename: Zhaolong
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Jiangsu Key Laboratory for Bioresources of Saline Soils, Provincial Key Laboratory of Agrobiology, Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences Nanjing, China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Wei
authors.affiliation: Jiangsu Key Laboratory for Bioresources of Saline Soils, Provincial Key Laboratory of Agrobiology, Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences Nanjing, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Xu
authors.affiliation: Jiangsu Key Laboratory for Bioresources of Saline Soils, Provincial Key Laboratory of Agrobiology, Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences Nanjing, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Wan
authors.affiliation: Jiangsu Key Laboratory for Bioresources of Saline Soils, Provincial Key Laboratory of Agrobiology, Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences Nanjing, China.
authors.forename: Yihong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Jiangsu Key Laboratory for Bioresources of Saline Soils, Provincial Key Laboratory of Agrobiology, Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences Nanjing, China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: He
authors.affiliation: Huaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu Huai'an, China.
authors.forename: Jiayin
authors.initials: J
authors.lastname: Yang
authors.affiliation: Jiangsu Key Laboratory for Bioresources of Saline Soils, Provincial Key Laboratory of Agrobiology, Institute of Biotechnology, Jiangsu Academy of Agricultural SciencesNanjing, China; Key Laboratory of Coastal Biology and Bioresources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of SciencesYantai, China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Shao
authors.affiliation: Jiangsu Key Laboratory for Bioresources of Saline Soils, Provincial Key Laboratory of Agrobiology, Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences Nanjing, China.
authors.forename: Hongxiang
authors.initials: H
authors.lastname: Ma
keywords: GmZFP3; Soybean; drought response; negatively; transgenic Arabidopsis