title: Liquid Growth of Arrayed Fluorescently Tagged Saccharomyces cerevisiae Strains for Live-Cell High-Throughput Microscopy Screens.
abstract: This protocol describes culturing arrays of fluorescently tagged yeast strains to early log-phase in a 96-well format for imaging on a high-throughput (HTP) microscope. The method assumes the use of the synthetic genetic array (SGA) technique to create the array of marked strains. When this approach is coupled with automated image analysis, the subcellular distribution and abundance of tagged proteins can be systematically and quantitatively examined in different genetic backgrounds and/or under different growth regimes.
authors.affiliation: The Donnelly Centre, University of Toronto, Toronto, Ontario, M5S 3E1 Canada;
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Cox
authors.affiliation: Janssen Pharmaceutica, Johnson & Johnson, Beerse, Belgium 2340.
authors.forename: Yolanda T
authors.initials: YT
authors.lastname: Chong
authors.affiliation: The Donnelly Centre, University of Toronto, Toronto, Ontario, M5S 3E1 Canada;
authors.forename: Charles
authors.initials: C
authors.lastname: Boone
authors.affiliation: The Donnelly Centre, University of Toronto, Toronto, Ontario, M5S 3E1 Canada;
authors.forename: Brenda
authors.initials: B
authors.lastname: Andrews
mesh_terms: D057205:Automation, Laboratory; D057166:High-Throughput Screening Assays; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods*; D061848:Optical Imaging / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000254:growth & development*; D013194:Staining and Labeling / Q000379:methods