title: Concanavaline A conjugated bacterial polyester-based PHBHHx nanoparticles loaded with curcumin for breast cancer therapy.
abstract: The aim of this study was to evaluate therapeutic potential of curcumin-loaded poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) PHBHHx nanoparticles (CUR-NPs) and concanavaline A conjugated curcumin-loaded NPs (ConA-CUR-NPs) for breast cancer treatment. The size and zeta potential of prepared NPs were about 228 ± 5 nm and -23.3 mV, respectively. The entrapment efficiencies of polymer/drug weight ratios, 1.25CUR-NPs, 2.5CUR-NPs, 5CUR-NPs, ConA-1.25CUR-NPs, ConA-2.5CUR-NPs and ConA-5CUR-NPs were found to be ≈68, 55, 45, 70, 60 and 51%, respectively. Optimized NPs formulations in the freeze-dried form were assessed with their short-term stability for 30 days of storage at 4 °C and 25 °C. Anticancer activity of ConA-CUR-NPs was proved by MTT assay and reconfirmed by double staining and flow cytometry results. The anticancer activity of ConA-CUR-NPs was measured in human breast cancer cells (MDA-MB 231) in vitro, and the results revealed that the ConA-CUR-NPs had better tumor cells decline activity.
authors.affiliation: a Zonguldak Vocational High School, Bülent Ecevit University , Zonguldak , Turkey ;
authors.forename: Ebru
authors.initials: E
authors.lastname: Kilicay
authors.affiliation: b Department of Biology, Faculty of Science , Aksaray University , Aksaray , Turkey ;
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Karahaliloglu
authors.affiliation: c Department of Chemistry, Physical Chemistry Division , Bülent Ecevit University , Zonguldak , Turkey ;
authors.forename: Baki
authors.initials: B
authors.lastname: Hazer
authors.affiliation: d Department of Medical Immunology, Faculty of Medicine , Bülent Ecevit University , Zonguldak , Turkey ;
authors.forename: Ishak Özel
authors.initials: IÖ
authors.lastname: Tekin
authors.affiliation: e Department of Chemistry, Biochemistry Division , Hacettepe University , Ankara , Turkey.
authors.forename: Emir Baki
authors.initials: EB
authors.lastname: Denkbas
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000494:pharmacology; D001940:Breast / Q000187:drug effects; D001943:Breast Neoplasms / Q000188:drug therapy; D032449:Canavalia / Q000737:chemistry; D002208:Caproates / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003208:Concanavalin A / Q000737:chemistry*; D003474:Curcumin / Q000008:administration & dosage* / Q000494:pharmacology; D003692:Delayed-Action Preparations / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D005260:Female; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D000094122:Polyhydroxybutyrates
chemical_list: D020155:3-Hydroxybutyric Acid; D000970:Antineoplastic Agents; D002208:Caproates; D003208:Concanavalin A; D003474:Curcumin; D003692:Delayed-Action Preparations; D004337:Drug Carriers; C115940:poly(3-hydroxybutyrate-co-3-hydroxyhexanoate); D000094122:Polyhydroxybutyrates
keywords: PHBHHx; PHBHHx nanoparticles; concanavaline A; curcumin; drug delivery; human breast cancer cells