title: Identification of Differentially Expressed microRNAs between the Fenpropathrin Resistant and Susceptible Strains in Tetranychus cinnabarinus.
abstract: The carmine spider mite (Tetranychus cinnabarinus) is one of the most serious pests on crops and its control mainly depends on chemical acaricides. The excessive and improper acaricides use has resulted in mite resistance to many acaricides, including fenpropathrin. Previous studies have indicated fenpropathrin resistance is a complex development process involving many genes, but information on resistance mechanism of post-transcription regulation is rare. Using Illumina sequencing, several categories of sRNAs were identified from susceptible (TS) and fenpropathrin-resistant strains (TR) of T. cinnabarinus, including 75 known microRNAs (miRNAs) and 64 novel miRNAs, whose target genes containing 78592 miRNA-target pairs were predicted by 6 algorithms. Also, 12 significantly differently expressed miRNAs were identified between the TS and TR libraries and RT-qPCR validation also performed a well consistency with sequencing. The targets of significantly differentially expressed miRNAs included 7 glutathione S-transferase, 7 cytochrome P450 and 16 carboxyl/choline esterase genes, their function in fenpropathrin resistance were further analyzed. The present study provides the firstly large-scale characterization of miRNAs in T. cinnabarinus and the comparison between TS and TR strains gives a clue on how miRNA involves in fenpropathrin resistance.
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
authors.forename: Yichao
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Peng
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
authors.forename: Yanchao
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Shi
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
authors.forename: Guangmao
authors.initials: G
authors.lastname: Shen
authors.affiliation: College of Horticulture and Landscape Agriculture, Southwest University, Chongqing, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
authors.forename: Lin
authors.initials: L
authors.lastname: He
mesh_terms: D056810:Acaricides / Q000494:pharmacology*; D000818:Animals; D004351:Drug Resistance / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics*; D011722:Pyrethrins / Q000494:pharmacology*; D040102:Tetranychidae / Q000187:drug effects* / Q000235:genetics
chemical_list: D056810:Acaricides; D035683:MicroRNAs; D011722:Pyrethrins; C044267:fenpropathrin