title: Caffeic Acid Inhibits Chronic UVB-Induced Cellular Proliferation Through JAK-STAT3 Signaling in Mouse Skin.
abstract: Signal transducers and activators of transcription 3 (STAT3) play a critical role in inflammation, proliferation and carcinogenesis. Inhibition of JAK-STAT3 signaling is proved to be a novel target for prevention of UVB-induced skin carcinogenesis. In this study, chronic UVB irradiation (180 mJ cm(-2) ; weekly thrice for 30 weeks) induces the expression of IL-10 and JAK1 that eventually activates the STAT3 which leads to the transcription of proliferative and antiapoptotic markers such as PCNA, Cyclin-D1, Bcl2 and Bcl-xl, respectively. Caffeic acid (CA) inhibits JAK-STAT3 signaling, thereby induces apoptotic cell death by upregulating Bax, Cytochrome-C, Caspase-9 and Caspase-3 expression in mouse skin. Furthermore, TSP-1 is an antiangiogeneic protein, which is involved in the inhibition of angiogenesis and proliferation. Chronic UVB exposure decreased the expression of TSP-1 and pretreatment with CA prevented the UVB-induced loss of TSP-1 in UVB-irradiated mouse skin. Thus, CA offers protection against UVB-induced photocarcinogenesis probably through modulating the JAK-STAT3 in the mouse skin.
authors.affiliation: Department of Biochemistry and Biotechnology, Annamalai University, Chidambaram, Tamilnadu, India.
authors.forename: Balupillai
authors.initials: B
authors.lastname: Agilan
authors.affiliation: Department of Biochemistry and Biotechnology, Annamalai University, Chidambaram, Tamilnadu, India.
authors.forename: N
authors.initials: N
authors.lastname: Rajendra Prasad
authors.affiliation: Department of Biochemistry and Biotechnology, Annamalai University, Chidambaram, Tamilnadu, India.
authors.forename: Govindasamy
authors.initials: G
authors.lastname: Kanimozhi
authors.affiliation: Department of Biochemistry and Biotechnology, Annamalai University, Chidambaram, Tamilnadu, India.
authors.forename: Ramasamy
authors.initials: R
authors.lastname: Karthikeyan
authors.affiliation: Department of Biochemistry and Biotechnology, Annamalai University, Chidambaram, Tamilnadu, India.
authors.forename: Muthusamy
authors.initials: M
authors.lastname: Ganesan
authors.affiliation: Department of Biochemistry and Biotechnology, Annamalai University, Chidambaram, Tamilnadu, India.
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Mohana
authors.affiliation: Bioinformatics Infrastructure Facility (BIF), University of Madras, Chennai, Tamilnadu, India.
authors.forename: Devadasan
authors.initials: D
authors.lastname: Velmurugan
authors.affiliation: Bioinformatics Infrastructure Facility (BIF), University of Madras, Chennai, Tamilnadu, India.
authors.forename: Dhanapalan
authors.initials: D
authors.lastname: Ananthakrishnan
mesh_terms: D000818:Animals; D017209:Apoptosis; D015415:Biomarkers; D002109:Caffeic Acids / Q000494:pharmacology*; D020134:Catalytic Domain; D049109:Cell Proliferation / Q000187:drug effects* / Q000528:radiation effects*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000528:radiation effects; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism; D053613:Janus Kinase 1 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008958:Models, Molecular; D011487:Protein Conformation; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects; D012867:Skin / Q000166:cytology; D014466:Ultraviolet Rays
chemical_list: D015415:Biomarkers; D002109:Caffeic Acids; C556062:IL10 protein, mouse; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; D016753:Interleukin-10; C507905:Jak1 protein, mouse; D053613:Janus Kinase 1; C040048:caffeic acid