title: Effects of β-glucan on ROS production and energy metabolism in yellow croaker (Pseudosciaena crocea) under acute hypoxic stress.
abstract: The aim of the present study was to evaluate the effect of β-glucan on acute hypoxia-induced oxidative stress and the changes in energy metabolism by determining ROS production, activities and mRNA levels of energy metabolism enzyme (PK, F-ATPase, SDH and MDH), and in gene expression of HIF-1α in the liver of large yellow croaker. Fish were injected with β-glucan at a dose of 0 or 5 mg kg(-1) body weight on 6, 4 and 2 days before exposed to 1.5 and 7.0 mg DO L(-1) for 48 h. The results showed that β-glucan enhanced survival rate and reduced ROS during the lethal hypoxic stress, indicating that β-glucan could ameliorate hypoxia-induced oxidative stress. Obtained results also showed that β-glucan could up-regulate activities and mRNA levels of PK, demonstrating that β-glucan increased anaerobic glycolysis capacity. Furthermore, a coordinated transcriptional regulation of energy metabolism enzyme genes was observed, suggesting that HIF-1α is required for regulating these genes. In conclusion, β-glucan could alleviate cute hypoxia-induced oxidative stress in large yellow croker by enhancing anaerobic glycolysis capacity, emphasizing a central role of transcription factor HIF-1α in the process.
authors.affiliation: National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan, 316000, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zeng
authors.affiliation: National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan, 316000, China.
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Wang
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361005, China.
authors.forename: Chun-Xiang
authors.initials: CX
authors.lastname: Ai
authors.affiliation: National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan, 316000, China.
authors.forename: Jia-Lang
authors.initials: JL
authors.lastname: Zheng
authors.affiliation: National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan, 316000, China. wucw08@126.com.
authors.forename: Chang-Wen
authors.initials: CW
authors.lastname: Wu
authors.affiliation: National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan, 316000, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Cai
mesh_terms: D000818:Animals; D004734:Energy Metabolism / Q000187:drug effects*; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D000860:Hypoxia / Q000235:genetics / Q000378:metabolism*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics; D008099:Liver / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010473:Perciformes / Q000235:genetics / Q000378:metabolism*; D011770:Pyruvate Kinase / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D047071:beta-Glucans / Q000494:pharmacology*
chemical_list: D029941:Fish Proteins; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D047071:beta-Glucans; D008291:Malate Dehydrogenase; D013385:Succinate Dehydrogenase; D011770:Pyruvate Kinase; D025261:Mitochondrial Proton-Translocating ATPases
keywords: HIF-1α; Hypoxic stress; Pseudosciaena crocea; β-Glucan