title: Ghrelin treatment prevents development of activity based anorexia in mice.
abstract: Stimulation of feeding is necessary for treatment of pathological conditions of chronic malnutrition due to anorexia. Ghrelin, a hunger hormone, is one of the candidate for pharmacological treatments of anorexia, but because of its instability in plasma has limited efficacy. We previously showed that plasmatic IgG protect ghrelin from degradation and that IgG from obese subjects and mice may increase ghrelin׳s orexigenic effect. In this study we tested if ghrelin alone or combined with IgG may improve feeding in chronically food-restricted mice with or without physical activity-based anorexia (ABA) induced by free access to a running wheel. Mice received a single daily intraperitoneal injection of ghrelin (1nM) together or not with total IgG (1nM) from obese ob/ob or lean mice before access to food during 8 days of 3h/day feeding time. We found that both ghrelin and ghrelin combined with IgG from obese, but not lean mice, prevented ABA, however, they were not able to diminish body weight loss. Physical activity was lower during the feeding period and was increased shortly after feeding in mice receiving ghrelin together with IgG from obese mice. In food-restricted mice without ABA, ghrelin treatments did not have significant effects on food intake. Thus, this study supports pharmacological use of ghrelin or ghrelin combined with IgG from obese animals for treatment of anorexia accompanied by elevated physical activity. The utility of combining ghrelin with protective IgG should be further determined in animal models of anorexia with unrestricted access to food.
authors.affiliation: Nutrition, Gut and Brain Laboratory, Inserm UMR1073, Rouen University, 76183 France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen 76183, France.
authors.forename: Romain
authors.initials: R
authors.lastname: Legrand
authors.affiliation: Nutrition, Gut and Brain Laboratory, Inserm UMR1073, Rouen University, 76183 France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen 76183, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Lucas
authors.affiliation: Nutrition, Gut and Brain Laboratory, Inserm UMR1073, Rouen University, 76183 France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen 76183, France.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Breton
authors.affiliation: Nutrition, Gut and Brain Laboratory, Inserm UMR1073, Rouen University, 76183 France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen 76183, France.
authors.forename: Saïda
authors.initials: S
authors.lastname: Azhar
authors.affiliation: Institute for Research and Innovation in Biomedicine (IRIB), Rouen 76183, France; Animal Behavioral Platform SCAC, Rouen University, Rouen 76183, France.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: do Rego
authors.affiliation: Nutrition, Gut and Brain Laboratory, Inserm UMR1073, Rouen University, 76183 France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen 76183, France; Department of Nutrition, Rouen University Hospital, 76183 Rouen, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Déchelotte
authors.affiliation: Nutrition, Gut and Brain Laboratory, Inserm UMR1073, Rouen University, 76183 France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen 76183, France; Department of Nutrition, Rouen University Hospital, 76183 Rouen, France.
authors.forename: Moïse
authors.initials: M
authors.lastname: Coëffier
authors.affiliation: Nutrition, Gut and Brain Laboratory, Inserm UMR1073, Rouen University, 76183 France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen 76183, France. Electronic address: Serguei.Fetissov@univ-rouen.fr.
authors.forename: Sergueï O
authors.initials: SO
authors.lastname: Fetissov
mesh_terms: D000818:Animals; D000855:Anorexia / Q000517:prevention & control* / Q000523:psychology; D019138:Antibodies, Blocking / Q000494:pharmacology; D001835:Body Weight / Q000187:drug effects; D031204:Caloric Restriction; D004435:Eating / Q000187:drug effects; D054439:Ghrelin / Q000037:antagonists & inhibitors / Q000097:blood / Q000627:therapeutic use*; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism; D007074:Immunoglobulin G / Q000276:immunology; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009043:Motor Activity; D009765:Obesity / Q000276:immunology
chemical_list: D019138:Antibodies, Blocking; D054439:Ghrelin; D007074:Immunoglobulin G
keywords: Anorexia nervosa; Appetite; Autoantibodies; Cachexia; Food intake; Ghrelin