title: Isotope Labeling Study of Retinal Chromophore Fragmentation.
abstract: Previous studies have shown that the gas-phase fragmentation of the retinal chromophore after S0-S1 photoexcitation results in a prominent fragment of mass 248 which cannot be explained by the cleavage of any single bond along the polyene chain. It was therefore theorized that the fragmentation mechanism involves a series of isomerizations and cyclization processes, and two mechanisms for these processes were suggested. Here we used isotope labeling MS-MS to provide conclusive support for the fragmentation mechanism suggested by Coughlan et al. (J. Phys. Chem. Lett. 2014, 5, 3195).
authors.affiliation: Institute of Nanotechnology and Advanced Materials, Bar-Ilan University , Ramat-Gan 5290002, Israel.
authors.forename: Lihi
authors.initials: L
authors.lastname: Musbat
authors.affiliation: Institute of Nanotechnology and Advanced Materials, Bar-Ilan University , Ramat-Gan 5290002, Israel.
authors.forename: Maria
authors.initials: M
authors.lastname: Nihamkin
authors.affiliation: Institute of Nanotechnology and Advanced Materials, Bar-Ilan University , Ramat-Gan 5290002, Israel.
authors.forename: Shany
authors.initials: S
authors.lastname: Ytzhak
authors.affiliation: Chemistry Department, Weizmann Institute of Science , Rehovot 978007, Israel.
authors.forename: Amiram
authors.initials: A
authors.lastname: Hirshfeld
authors.affiliation: Chemistry Department, Weizmann Institute of Science , Rehovot 978007, Israel.
authors.forename: Noga
authors.initials: N
authors.lastname: Friedman
authors.affiliation: Spectrum Warfare Systems Department, NSWC Crane Division , Crane, Indiana 47522, United States.
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Dilger
authors.affiliation: Chemistry Department, Weizmann Institute of Science , Rehovot 978007, Israel.
authors.forename: Mordechai
authors.initials: M
authors.lastname: Sheves
authors.affiliation: Institute of Nanotechnology and Advanced Materials, Bar-Ilan University , Ramat-Gan 5290002, Israel.
authors.forename: Yoni
authors.initials: Y
authors.lastname: Toker
mesh_terms: D003500:Cyclization; D007553:Isotope Labeling; D012160:Retina / Q000737:chemistry*; D012243:Rhodopsin / Q000737:chemistry*; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry
chemical_list: D012243:Rhodopsin