title: Screening for toxic phorbol esters in jerky pet treat products using LC-MS.
abstract: Since 2007, the U.S. FDA's Center for Veterinary Medicine (CVM) has been investigating reports of pets becoming ill after consuming jerky pet treats. Jerky used in pet treats contains glycerin, which can be made from vegetable oil or as a byproduct of biodiesel production. Because some biodiesel is produced using oil from Jatropha curcas, a plant that contains toxic compounds including phorbol esters, CVM developed a liquid chromatography-mass spectrometry (LC-MS) screening method to evaluate investigational jerky samples for the presence of these toxins. Results indicated that the samples analyzed with the new method did not contain Jatropha toxins at or above the lowest concentration tested.
authors.affiliation: Division of Residue Chemistry, Food and Drug Administration, Center for Veterinary Medicine, Office of Research, 8401 Muirkirk Road, Laurel, MD 20708, USA.
authors.forename: Upul
authors.initials: U
authors.lastname: Nishshanka
authors.affiliation: Division of Residue Chemistry, Food and Drug Administration, Center for Veterinary Medicine, Office of Research, 8401 Muirkirk Road, Laurel, MD 20708, USA.
authors.forename: Hiranthi
authors.initials: H
authors.lastname: Jayasuriya
authors.affiliation: Division of Residue Chemistry, Food and Drug Administration, Center for Veterinary Medicine, Office of Research, 8401 Muirkirk Road, Laurel, MD 20708, USA.
authors.forename: Chaitali
authors.initials: C
authors.lastname: Chattopadhaya
authors.affiliation: Division of Residue Chemistry, Food and Drug Administration, Center for Veterinary Medicine, Office of Research, 8401 Muirkirk Road, Laurel, MD 20708, USA.
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Kijak
authors.affiliation: Division of Residue Chemistry, Food and Drug Administration, Center for Veterinary Medicine, Office of Research, 8401 Muirkirk Road, Laurel, MD 20708, USA.
authors.forename: Pak-Sin
authors.initials: PS
authors.lastname: Chu
authors.affiliation: Veterinary Laboratory Investigation & Response Network, Food and Drug Administration, Center for Veterinary Medicine, Office of Research, 8401 Muirkirk Road, Laurel, MD 20708, USA.
authors.forename: Renate
authors.initials: R
authors.lastname: Reimschuessel
authors.affiliation: Veterinary Laboratory Investigation & Response Network, Food and Drug Administration, Center for Veterinary Medicine, Office of Research, 8401 Muirkirk Road, Laurel, MD 20708, USA.
authors.forename: Andriy
authors.initials: A
authors.lastname: Tkachenko
authors.affiliation: Veterinary Laboratory Investigation & Response Network, Food and Drug Administration, Center for Veterinary Medicine, Office of Research, 8401 Muirkirk Road, Laurel, MD 20708, USA.
authors.forename: Olgica
authors.initials: O
authors.lastname: Ceric
authors.affiliation: Division of Residue Chemistry, Food and Drug Administration, Center for Veterinary Medicine, Office of Research, 8401 Muirkirk Road, Laurel, MD 20708, USA. Electronic address: hemakanthi.dealwis@fda.hhs.gov.
authors.forename: Hemakanthi G
authors.initials: HG
authors.lastname: De Alwis
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D056804:Biofuels / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods*; D031286:Jatropha / Q000737:chemistry; D016014:Linear Models; D013058:Mass Spectrometry / Q000379:methods*; D010703:Phorbol Esters / Q000032:analysis* / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry
chemical_list: D056804:Biofuels; D010703:Phorbol Esters; D010938:Plant Oils
keywords: Accurate mass; High resolution; Jatropha curcas; Jerky pet treats; LC–MS method; Phorbol esters