title: In Vitro Cytotoxicity Test and Surface Characterization of CoCrW Alloy in Artificial Saliva Solution for Dental Applications.
abstract: In order to evaluate its application as a dental prosthesis material, a CoCrW alloy was subjected to in vitro cytotoxicity test, surface characterization and electrochemical studies performed in artificial saliva and 0.15 mol.L-1 NaCl medium. The used techniques were: anodic polarization curves, chronoamperometric measurements, electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) analysis and X-ray photoelectron spectroscopy (XPS). Cytotoxicity test was also performed. The electrochemical behavior of CoCrW alloy was compared in both studied media, from corrosion potential (Ecorr) to a 600 mV anodic overvoltage. From the electrochemical measurements it was observed that the CoCrW alloy in both media presents only generalized corrosion. SEM and EDS analysis showed that the alloy presents carbide niobium and silicon and manganese oxides as nonmetallic inclusions. XPS results indicated that cobalt does not significantly contribute to the passivating film formation. Cytotoxicity test showed no cytotoxic character of CoCrW alloy. These results suggest that the CoCrW alloy can be used as biomaterial to be applied as prosthesis in dental implants.
authors.affiliation: Institute of Chemistry, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Klester Santos
authors.initials: KS
authors.lastname: Souza
authors.affiliation: Universidade Federal do ABC, Santo André, SP, Brazil.
authors.forename: Ruth Flavia Vera Villamil
authors.initials: RF
authors.lastname: Jaimes
authors.affiliation: Instituto de Pesquisas Energéticas e Nucleares, São Paulo, SP, Brazil.
authors.forename: Sizue Otta
authors.initials: SO
authors.lastname: Rogero
authors.affiliation: Department of Materials Engineering, Universidade Federal de São Carlos, São Carlos, SP, Brazil.
authors.forename: Pedro Augusto de Paula
authors.initials: PA
authors.lastname: Nascente
authors.affiliation: Institute of Chemistry, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Silvia Maria Leite
authors.initials: SM
authors.lastname: Agostinho
mesh_terms: D003722:Dental Alloys / Q000737:chemistry*; D066298:In Vitro Techniques; D012464:Saliva, Artificial / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D003722:Dental Alloys; D012464:Saliva, Artificial