title: Streptomyces indoligenes sp. nov., isolated from rhizosphere soil of Populus euphratica.
abstract: A novel actinobacterium, designated TRM 43006T, was isolated from the rhizosphere soil of Populus euphratica in Xinjiang Province, north-west China. Phylogenetic and phenotypic analysis demonstrated that strain TRM 43006T belongs to the genus Streptomyces. The strain was aerobic and Gram-stain-positive; the aerial mycelium branched monopodially, forming chains of arthrospores. The spores were oval to cylindrical with smooth surfaces. The whole-cell sugar pattern of strain TRM 43006T consisted of xylose, mannitol, galactose and ribose. The menaquinones were MK-9(H6), MK-9(H8) and MK-9(H10). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannosides and four unknown phospholipids. Major fatty acids were iso-C16 : 0, iso-C16 : 1, iso-C14 : 0 and anteiso-C15 : 0. The G+C content of the genomic DNA was 69.0 mol%. Comparative 16S rRNA gene sequence analysis indicated that strain TRM 43006T was phylogenetically most closely related to Streptomyces roseolilacinus NBRC 12815T (98.6 % similarity) and Streptomycessudanensis SD 504T (98.3 %); however, DNA-DNA hybridization studies between S. roseolilacinus NBRC 12815T, S. sudanensis SD 504T and TRM 43006T showed only 30.28 and 30.65  % relatedness, respectively. Based on the evidence from this polyphasic study, strain TRM 43006T represents a novel species of the genus Streptomyces, for which the name Streptomyces indoligenes sp. nov. is proposed. The type strain is TRM 43006T (=KCTC 39611T=CCTCC AA 2015010T).
authors.affiliation: Key Laboratory of Protection and Utilization of Biological Resoures in Tarim Basin of Xinjiang Production & Construction Corps/College of Life Science, Tarim University, Alar 843300, China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Luo
authors.affiliation: Key Laboratory of Protection and Utilization of Biological Resoures in Tarim Basin of Xinjiang Production & Construction Corps/College of Life Science, Tarim University, Alar 843300, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Key Laboratory of Protection and Utilization of Biological Resoures in Tarim Basin of Xinjiang Production & Construction Corps/College of Life Science, Tarim University, Alar 843300, China.
authors.forename: Sinan
authors.initials: S
authors.lastname: Xie
authors.affiliation: Key Laboratory of Protection and Utilization of Biological Resoures in Tarim Basin of Xinjiang Production & Construction Corps/College of Life Science, Tarim University, Alar 843300, China.
authors.forename: Chuanxing
authors.initials: C
authors.lastname: Wan
authors.affiliation: Key Laboratory of Protection and Utilization of Biological Resoures in Tarim Basin of Xinjiang Production & Construction Corps/College of Life Science, Tarim University, Alar 843300, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D032107:Populus / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9