title: Human lung fibroblasts may modulate dendritic cell phenotype and function: results from a pilot in vitro study.
abstract: In human lung fibrotic lesions, fibroblasts were shown to be closely associated with immature dendritic cell (DC) accumulation. The aim of the present pilot study was to characterize the role of pulmonary fibroblasts on DC phenotype and function, using co-culture of lung fibroblasts from patients with idiopathic pulmonary fibrosis (IPF) and from control patients, with a DC cell line MUTZ-3. We observed that co-culture of lung control and IPF fibroblasts with DCs reduced the expression of specific DC markers and down-regulated their T-cell stimulatory activity. This suggests that pulmonary fibroblasts might sustain chronic inflammation in the fibrotic lung by maintaining in situ a pool of immature DCs.
authors.affiliation: Inserm U 1152, 46, rue Henri Huchard, Paris, 75018, France.|Université Paris Diderot, Sorbonne Paris Cité, Paris, France.|DHU FIRE, Paris, France.|Assistance Publique-Hôpitaux de Paris, Hôpital Bichat, Service de Pneumologie A, Paris, France.
authors.forename: Olivia
authors.initials: O
authors.lastname: Freynet
authors.affiliation: Inserm U 1152, 46, rue Henri Huchard, Paris, 75018, France.|Université Paris Diderot, Sorbonne Paris Cité, Paris, France.|DHU FIRE, Paris, France.
authors.forename: Joëlle
authors.initials: J
authors.lastname: Marchal-Sommé
authors.affiliation: Inserm UMR-S 976, Université Paris Diderot, Sorbonne Paris Cité, Hôpital Saint Louis, Paris, France.
authors.forename: Francette
authors.initials: F
authors.lastname: Jean-Louis
authors.affiliation: Inserm U 1152, 46, rue Henri Huchard, Paris, 75018, France.|Université Paris Diderot, Sorbonne Paris Cité, Paris, France.|DHU FIRE, Paris, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Mailleux
authors.affiliation: Inserm U 1152, 46, rue Henri Huchard, Paris, 75018, France. bruno.crestani@bch.aphp.fr.|Université Paris Diderot, Sorbonne Paris Cité, Paris, France. bruno.crestani@bch.aphp.fr.|DHU FIRE, Paris, France. bruno.crestani@bch.aphp.fr.|Assistance Publique-Hôpitaux de Paris, Hôpital Bichat, Service de Pneumologie A, Paris, France. bruno.crestani@bch.aphp.fr.|Service de Pneumologie, Hôpital Bichat, 46, rue Henri Huchard, Paris cedex 18, 75018, France. bruno.crestani@bch.aphp.fr.
authors.forename: Bruno
authors.initials: B
authors.lastname: Crestani
authors.affiliation: Inserm U 1152, 46, rue Henri Huchard, Paris, 75018, France.|Université Paris Diderot, Sorbonne Paris Cité, Paris, France.|DHU FIRE, Paris, France.
authors.forename: Paul
authors.initials: P
authors.lastname: Soler
authors.affiliation: Inserm UMR-S 976, Université Paris Diderot, Sorbonne Paris Cité, Hôpital Saint Louis, Paris, France. laurence.michel@inserm.fr.|Inserm UMR-S 976, Hôpital Saint-Louis, 1 avenue Claude Vellefaux, 75475, Paris, 75010, France. laurence.michel@inserm.fr.
authors.forename: Laurence
authors.initials: L
authors.lastname: Michel
mesh_terms: D002450:Cell Communication / Q000276:immunology*; D049109:Cell Proliferation / Q000502:physiology; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology* / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D008168:Lung / Q000166:cytology* / Q000276:immunology*; D010641:Phenotype; D010865:Pilot Projects
chemical_list: D016207:Cytokines
keywords: Dendritic cells; Fibroblasts; Fibrosis; Lung