title: Effect of IL-7 and IL-15 on T cell phenotype in myelodysplastic syndromes.
abstract: Aberrant T cell phenotype is one of the characteristics of myelodysplastic syndromes (MDS). In this study, we detected an increased concentration of IL-15 in the plasma of MDS patients (n = 20) compared with that in the plasma of healthy controls (n = 20). In MDS patients, reduced naïve CD4+ and CD8+ T cells [16.11 ± 6.56 vs. 24.11 ± 7.18 for CD4+ T cells (p < 0.001) and 13.15 ± 5.67 vs. 23.51 ± 6.25 for CD8+ T cells (p < 0.001)] were observed. The reduced naïve and increased effector memory T cells were significantly correlated with IL-15 plasma level. Then, the effect of IL-15 and IL-7 was tested in vitro. Peripheral blood mononuclear cells from MDS were treated for 15 days with IL-15. This treatment significantly decreased naïve CD4+ (p < 0.001) and CD8+ (p < 0.001) T cells and correspondingly increased terminal memory CD4+ and CD8+ T cells (p < 0.001). Treatment with IL-7 increased naïve CD4+ (p < 0.05) and CD8+ (p < 0.001) T cells. Our results indicated that exposure to high levels of IL-15 may be involved in the T cell phenotype conversion observed in MDS. IL-7 may be one of the promising therapeutic candidates for recovering the effector immune compartment in MDS patients.
authors.affiliation: Department of Orthopaedic Surgery, Tianjin Hongqiao Hospital, Tianjin, P.R. China.
authors.forename: Wen
authors.initials: W
authors.lastname: Dong
authors.affiliation: Department of Immunology, Tianjin Cancer Institute and Hospital, Tianjin Medical University, Tianjin, P.R. China.|National Clinical Research Center of Cancer, P.R. China.|Tianjin Key Laboratory of Cancer Immunology and Biotherapy, Tianjin, P.R. China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Ding
authors.affiliation: Department of Immunology, Tianjin Cancer Institute and Hospital, Tianjin Medical University, Tianjin, P.R. China.|National Clinical Research Center of Cancer, P.R. China.|Tianjin Key Laboratory of Cancer Immunology and Biotherapy, Tianjin, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Immunology, Tianjin Cancer Institute and Hospital, Tianjin Medical University, Tianjin, P.R. China.|National Clinical Research Center of Cancer, P.R. China.|Tianjin Key Laboratory of Cancer Immunology and Biotherapy, Tianjin, P.R. China.
authors.forename: Xiubao
authors.initials: X
authors.lastname: Ren
authors.affiliation: Immunology Program, H. Lee Moffitt Cancer Center, Tampa, FL, USA.
authors.forename: P K
authors.initials: PK
authors.lastname: Epling-Burnette
authors.affiliation: Department of Immunology, Tianjin Cancer Institute and Hospital, Tianjin Medical University, Tianjin, P.R. China.|National Clinical Research Center of Cancer, P.R. China.|Tianjin Key Laboratory of Cancer Immunology and Biotherapy, Tianjin, P.R. China.
authors.forename: Lili
authors.initials: L
authors.lastname: Yang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007156:Immunologic Memory / Q000187:drug effects / Q000276:immunology; D019409:Interleukin-15 / Q000276:immunology* / Q000494:pharmacology; D015851:Interleukin-7 / Q000097:blood / Q000276:immunology* / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000097:blood / Q000276:immunology*; D010641:Phenotype; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
chemical_list: D019409:Interleukin-15; D015851:Interleukin-7
keywords: IL-15; IL-7; T cells; myelodysplastic syndromes