title: Cold plasma inactivation of chronic wound bacteria.
abstract: Cold plasma is partly ionized non-thermal plasma generated at atmospheric pressure. It has been recognized as an alternative approach in medicine for sterilization of wounds, promotion of wound healing, topical treatment of skin diseases with microbial involvement and treatment of cancer. Cold plasma used in wound therapy inhibits microbes in chronic wound due to its antiseptic effects, while promoting healing by stimulation of cell proliferation and migration of wound relating skin cells. In this study, two types of plasma systems are employed to generate cold plasma: a parallel plate dielectric barrier discharge and a capillary-guided corona discharge. Parameters such as applied voltage, discharge frequency, treatment time and the flow of the carrier gas influence the cold plasma chemistry and therefore change the composition and concentration of plasma species that react with the target sample. Chronic wound that fails to heal often infected by multidrug resistant organisms makes them recalcitrant to healing. Methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa (Pseudomonas aeruginosa) are two common bacteria in infected and clinically non-infected wounds. The efficacies of the cold plasma generated by the two designs on the inactivation of three different isolates of MRSA and four isolates of P. aeruginosa are reported here.
authors.affiliation: Plasma Research Laboratory, Department of Physics, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: N
authors.initials: N
authors.lastname: Mohd Nasir
authors.affiliation: Plasma Research Laboratory, Department of Physics, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: B K
authors.initials: BK
authors.lastname: Lee
authors.affiliation: Faculty of Engineering, Multimedia University, Cyberjaya, 63100, Selangor, Malaysia.
authors.forename: S S
authors.initials: SS
authors.lastname: Yap
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: K L
authors.initials: KL
authors.lastname: Thong
authors.affiliation: Plasma Research Laboratory, Department of Physics, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia. Electronic address: yapsl@um.edu.my.
authors.forename: S L
authors.initials: SL
authors.lastname: Yap
mesh_terms: D001274:Atmospheric Pressure; D001424:Bacterial Infections / Q000517:prevention & control; D049109:Cell Proliferation; D003080:Cold Temperature; D024881:Drug Resistance, Bacterial; D006801:Humans; D008712:Methicillin / Q000737:chemistry; D058626:Plasma Gases; D011550:Pseudomonas aeruginosa; D013211:Staphylococcus aureus; D014945:Wound Healing; D014947:Wounds and Injuries / Q000382:microbiology* / Q000628:therapy
chemical_list: D058626:Plasma Gases; D008712:Methicillin
keywords: Chronic wound bacteria inactivation; Cold plasma; Corona discharge; Dielectric barrier discharge; Non thermal plasma; Wound healing