title: A reversible and highly selective fluorescence "on-off-on" probe for detecting nickel ion in the mitochondria of living cells.
abstract: A simple molecular fluorescent probe L has been designed, synthesized and characterized. The probe upon interaction with different metals ions show high selectivity and sensitivity for Ni(2+) through fluorescence "turn-off" response in acetonitrile, and the fluorescence could be recovered when chelating agent TPEN is added in this system, viz an "on-off-on" process. Its recognition mechanism has been established by fluorescence spectra, (1)H NMR study. Moreover, to further explore its utility in biological system, L is selected as a probe for Ni(2+) indicator in live cells and shows sensitivity on Ni(2+) intracellular distribution. Fluorescence co-localization studies demonstrate that L is a specific mitochondria-targeted fluorescent probe with excellent cell membrane permeability. Furthermore, a rapidly reversible fluorescence imaging of probe L detecting Ni(2+) in the mitochondria is successfully achieved. These facts would make this probe a great potential for detecting Ni(2+) in biology.
authors.affiliation: College of Chemistry and Chemical Engineering, Anhui University and Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, 230601 Hefei, PR China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Yu
authors.affiliation: Center of Stem Cell Research and Transformation Medicine, Anhui University, Hefei 230601, PR China.
authors.forename: Xiaohe
authors.initials: X
authors.lastname: Tian
authors.affiliation: College of Chemistry and Chemical Engineering, Anhui University and Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, 230601 Hefei, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Chemistry and Chemical Engineering, Anhui University and Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, 230601 Hefei, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Chemistry and Chemical Engineering, Anhui University and Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, 230601 Hefei, PR China.
authors.forename: Weiju
authors.initials: W
authors.lastname: Zhu
authors.affiliation: College of Chemistry and Chemical Engineering, Anhui University and Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, 230601 Hefei, PR China. Electronic address: zhpzhp@263.net.
authors.forename: Hongping
authors.initials: H
authors.lastname: Zhou
authors.affiliation: College of Chemistry and Chemical Engineering, Anhui University and Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, 230601 Hefei, PR China.
authors.forename: Yupeng
authors.initials: Y
authors.lastname: Tian
authors.affiliation: College of Chemistry and Chemical Engineering, Anhui University and Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, 230601 Hefei, PR China.
authors.forename: Jieying
authors.initials: J
authors.lastname: Wu
mesh_terms: D015374:Biosensing Techniques; D002413:Cations, Divalent / Q000032:analysis; D005456:Fluorescent Dyes / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods; D008928:Mitochondria / Q000737:chemistry*; D009532:Nickel / Q000032:analysis*; D061848:Optical Imaging / Q000379:methods*; D013050:Spectrometry, Fluorescence
chemical_list: D002413:Cations, Divalent; D005456:Fluorescent Dyes; D009532:Nickel
keywords: Biological application; Fluorescence probe; Mitochondria; Nickel ion; Recognition mechanism