title: Where have all the inosines gone? Conflicting evidence for A-to-I editing of the anticodon of higher eukaryotic tRNAACGArg questions the dogma of a universal wobble-mediated decoding of CGN codons.
abstract: Codon-anticodon recognition between triplets of an mRNA and a specific tRNA is the key element in the translation of the genetic code. In general, the precision of this process is dominated by a strict Watson-Crick base-pairing scheme. However, the degeneracy of the genetic code led Crick to propose the Wobble Hypothesis, permitting a less restraining interaction with the third base of the codon and involving the participation of inosine for decoding C-ending codons. The concept that the anticodon base A34 of tRNAACGArg in all eukaryotes, eubacteria, and plant chloroplasts is converted to I34 is firmly anchored in the literature despite conflicting evidence for its existence in higher eukaryote cytoplasmic tRNAACGArg. Here, we provide additional data and summarize the arguments favoring and contradicting post-transcriptional deamination of this position. A hypothesis that resolves the apparent conflict is proposed. © 2016 IUBMB Life, 68(6):419-422, 2016.
authors.affiliation: Institut für Biologie III, Universität Freiburg, Freiburg, Germany.
authors.forename: Gabor L
authors.initials: GL
authors.lastname: Igloi
authors.affiliation: Institut für Biologie III, Universität Freiburg, Freiburg, Germany.
authors.forename: Carolin A
authors.initials: CA
authors.lastname: Aldinger
mesh_terms: D000241:Adenosine / Q000378:metabolism; D000926:Anticodon; D003062:Codon; D003593:Cytoplasm / Q000235:genetics / Q000378:metabolism; D005057:Eukaryotic Cells; D005815:Genetic Code; D006801:Humans; D007288:Inosine / Q000235:genetics* / Q000378:metabolism; D017393:RNA Editing; D012347:RNA, Transfer, Arg / Q000235:genetics / Q000378:metabolism*
chemical_list: D000926:Anticodon; D003062:Codon; D012347:RNA, Transfer, Arg; D007288:Inosine; D000241:Adenosine
keywords: RNA editing; import of tRNAs into mitochondria; protein synthesis; transfer RNAs and aminoacyl-tRNA synthetases