title: Dickeyafangzhongdai sp. nov., a plant-pathogenic bacterium isolated from pear trees (Pyrus pyrifolia).
abstract: Gram-stain-negative, pectinolytic bacteria were repeatedly isolated from pear trees displaying symptoms of bleeding canker in China. Three strains, JS5T, LN1 and QZH3, had identical 16S rRNA gene sequences that shared 99 % similarity to the type strain of Dickeya dadantii. Phylogenetic analysis of strains JS5T, LN1 and QZH3 with isolates representing all species of the genus Dickeya and related Pectobacterium species supported their affiliation to Dickeya. Multi-locus sequence typing employing concatenated sequences encoding recA, fusA, gapA, purA, rplB, dnaX and the intergenic spacer illustrated a phylogeny which placed strains JS5T, LN1 and QZH3 as a distinct clade, separate from all other species of the genus Dickeya. Average nucleotide identity values obtained in comparison with all species of the genus Dickeya supported the distinctiveness of strain JS5T within the genus Dickeya. Additionally, all three strains were phenotypically distinguished from other species of the genus Dickeya by failing to hydrolyse casein, and by producing acids from (-)-d-arabinose, (+)melibiose, (+)raffinose, mannitol and myo-inositol, but not from 5-keto-d-gluconate or β-gentiobiose. The name Dickeya fangzhongdai sp. nov. is proposed to accommodate these strains; the type strain is JS5T (=CGMCC 1.15464T=DSM 101947T).
authors.affiliation: College of Plant Protection and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Shanghai Agricultural Technology Extension and Service Center, Shanghai 201103, PR China.
authors.forename: Yuqiang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Charlottetown Laboratory, Canadian Food Inspection Agency, Charlottetown, Canada.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135, PR China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Yi
authors.affiliation: College of Plant Protection and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Jiaqin
authors.initials: J
authors.lastname: Fan
authors.affiliation: College of Plant Protection and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Xu
authors.affiliation: College of Plant Protection and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Baishi
authors.initials: B
authors.lastname: Hu
authors.affiliation: Charlottetown Laboratory, Canadian Food Inspection Agency, Charlottetown, Canada.
authors.forename: Solke H
authors.initials: SH
authors.lastname: De Boer
authors.affiliation: Charlottetown Laboratory, Canadian Food Inspection Agency, Charlottetown, Canada.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
mesh_terms: D015373:Bacterial Typing Techniques; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004755:Enterobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D058885:Multilocus Sequence Typing; D010641:Phenotype; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D031989:Pyrus / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S