title: Knockdown of retinoblastoma protein may sensitize glioma cells to cisplatin through inhibition of autophagy.
abstract: Glioblastoma multiforme (GBM) is one of the deadliest forms of cancer due to its limited sensitivity to chemotherapy and radiotherapy. Cisplatin (CCDP) is a widely used chemotherapeutic agent for tumors, but the agent often results in the development of chemo-resistance. In several cancers, cisplatin resistance is associated with autophagy induction. Here, we found that in glioma cells cisplatin treatment induced autophagy. Our data indicates that the autophagy induction plays a critical role in cisplatin resistance of glioma cells, knockdown of RB inhibited autophagy induced by cisplatin, and inhibition of autophagy improved cisplatin-induced apoptosis. It suggests that a combination of autophagy inhibitors with cisplatin may improve the therapeutic efficiency of cisplatin towards GBM with acquired resistance.
authors.affiliation: Department of Neurosurgery, Nanjing Jinling Hospital, School of Medicine, Nanjing University, Nanjing, Jiangsu, PR China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Nanjing Jinling Hospital, School of Medicine, Nanjing University, Nanjing, Jiangsu, PR China.
authors.forename: Kangjian
authors.initials: K
authors.lastname: Sun
authors.affiliation: Department of Neurosurgery, Nanjing Jinling Hospital, School of Medicine, Nanjing University, Nanjing, Jiangsu, PR China.
authors.forename: Handong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Surgery, School of Medicine, University of California, San Francisco, United States. Electronic address: yuyuan.dai@ucsf.edu.
authors.forename: Yuyuan
authors.initials: Y
authors.lastname: Dai
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D001343:Autophagy / Q000187:drug effects*; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm; D055785:Gene Knockdown Techniques; D005910:Glioma / Q000235:genetics / Q000473:pathology*; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D016160:Retinoblastoma Protein / Q000235:genetics*
chemical_list: D000970:Antineoplastic Agents; D034741:RNA, Small Interfering; D016160:Retinoblastoma Protein; D002945:Cisplatin
keywords: Apoptosis; Autophagy; Cisplatin; Glioblastoma; Retinoblastoma protein