title: The Comparison of Adipose Stem Cell and Placental Stem Cell in Secretion Characteristics and in Facial Antiaging.
abstract: Background. Mesenchymal stem cells are the most commonly used seed cells in biomedical research and tissue engineering. Their secretory proteins have also been proven to play an important role in tissue healing. Methods. We isolated adipose stem cells and placental stem cells and performed analysis examining characteristics. The secretory proteins were extracted from conditioned medium and analyzed by MALDI-TOF/TOF. The antiaging effect of conditioned mediums was evaluated by the results of facial skin application. Results. Adipose stem cells and placental stem cells were found to be very similar in their surface markers and multipotency. The specific proteins secreted from adipose stem cells were more adept at cell adhesion, migration, wound healing, and tissue remodeling, while the proteins secreted by placental stem cells were more adept at angiogenesis, cell proliferation, differentiation, cell survival, immunomodulation, and collagen degradation. While these two types of conditioned medium could improve the facial index, the improvement of Melanin index after injection of the adipose stem cell conditioned medium was much more significant. Conclusion. The results suggest that the secreted proteins are ideal cell-free substances for regeneration medicine, especially in the antiaging field.
authors.affiliation: Nanjing Regenerative Medicine Engineering and Technology Research Center, Nanjing 210046, China; Regenerative Medicine Center, The Second Military Medical University, Shanghai 200433, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Nanjing Regenerative Medicine Engineering and Technology Research Center, Nanjing 210046, China; Regenerative Medicine Center, The Second Military Medical University, Shanghai 200433, China.
authors.forename: Shilei
authors.initials: S
authors.lastname: Guo
authors.affiliation: Nanjing Regenerative Medicine Engineering and Technology Research Center, Nanjing 210046, China.
authors.forename: Cui
authors.initials: C
authors.lastname: Wei
authors.affiliation: Nanjing Regenerative Medicine Engineering and Technology Research Center, Nanjing 210046, China.
authors.forename: Honglan
authors.initials: H
authors.lastname: Li
authors.affiliation: Nanjing Regenerative Medicine Engineering and Technology Research Center, Nanjing 210046, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.affiliation: Nanjing Regenerative Medicine Engineering and Technology Research Center, Nanjing 210046, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Yin
authors.affiliation: Regenerative Medicine Center, The Second Military Medical University, Shanghai 200433, China.
authors.forename: Chuansen
authors.initials: C
authors.lastname: Zhang