title: Molecular structure, chemical properties and biological activities of Pinto bean pod polysaccharide.
abstract: Pinto bean pod polysaccharide (PBPP) was successfully extracted with yield of 38.5g/100g and the PBPP gave total carbohydrate and uronic acid contents of 286.2mg maltose equivalent/g and 374.3mgGal/g, respectively. The Mw of PBPP was 270.6kDa with intrinsic viscosity of 0.262dm(3)/g, which composed of mannose (2.5%), galacturonic acid (15.0%), rhamnose (4.0%), glucose (9.0%), galactose (62.2%), xylose (2.9%) and arabinose (4.3%) with trace amount of ribose and fucose. The result suggested that PBPP has a spherical conformation with a highly branched structure. Fourier Transform Infrared analysis showed that PBPP has a similar structure as commercial pectin with an esterification degree of 59.9%, whereas scanning electron microscopy study showed that the crude polysaccharide formed a thin layer of film that was made of multiple micro strands of fibre. PBPP exhibited substantial free radical scavenging activity (7.7%), metal reducing capability (2.04mmol/dm(3)) and α-amylase inhibitory activity (97.6%) at a total amount of 1mg. PBPP also exhibited high water- and oil-holding capacities (3.6g/g and 2.8g/g, respectively). At a low concentration, PBPP exhibited emulsifying activity of 39.6% with stability of 38.6%. Apart from that, PBPP was able to show thickening capability at low concentration (0.005kg/dm(3)).
authors.affiliation: Analytical Biochemistry Research Centre, 11800 USM, Penang, Malaysia.
authors.forename: Fazlina
authors.initials: F
authors.lastname: Kamarudin
authors.affiliation: Analytical Biochemistry Research Centre, 11800 USM, Penang, Malaysia. Electronic address: mattgan81@gmail.com.
authors.forename: Chee-Yuen
authors.initials: CY
authors.lastname: Gan
mesh_terms: D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D007887:Fabaceae / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification; D006601:Hexoses / Q000737:chemistry / Q000302:isolation & purification; D006603:Hexuronic Acids / Q000737:chemistry / Q000302:isolation & purification; D006868:Hydrolysis; D008320:Maltose / Q000737:chemistry / Q000302:isolation & purification; D015394:Molecular Structure; D010851:Picrates / Q000037:antagonists & inhibitors / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D000516:alpha-Amylases / Q000037:antagonists & inhibitors / Q000737:chemistry
chemical_list: D001713:Biphenyl Compounds; D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D006601:Hexoses; D006603:Hexuronic Acids; D010851:Picrates; D011134:Polysaccharides; C007819:galacturonic acid; D008320:Maltose; C004931:1,1-diphenyl-2-picrylhydrazyl; D000516:alpha-Amylases
keywords: Pod; Polysaccharide; α-Amylase inhibitor