title: The Effects of Different Purifying Methods on the Chemical Properties, in Vitro Anti-Tumor and Immunomodulatory Activities of Abrus cantoniensis Polysaccharide Fractions.
abstract: Abrus cantoniensis (Hance) is a popular Chinese vegetable consumed as a beverage, soup or folk medicine. To fully exploit the potential of the polysaccharide in Abrus cantoniensis, nine polysaccharide fractions of Abrus cantoniensis were isolated and purified (AP-AOH30-1, AP-AOH30-2, AP-AOH80-1, AP-AOH80-2, AP-ACl-1, AP-ACl-2, AP-ACl-3, AP-H and AP-L). Fourier-transform infrared spectroscopy (FT-IR) and gas chromatography (GC) were used to characterize these Abrus polysaccharides fractions (APF). In vitro anti-tumor and immunomodulatory activities were also investigated and compared using the rank-sum ratio (RSR) method. Results demonstrated significant differences in the structure and bioactivities among APF, which were associated to the process used for their purification. Among the APF, AP-ACl-3 yield was 613.5 mg/kg of product and consisted of rhamnose (9.8%), arabinose (8.9%), fructose (3.0%), galactose (9.9%), glucose (4.3%), galacturonic acid (3.0%) and glucuronic acid (61.1%) with a molecular weight of 4.4 × 10⁴ Da. Furthermore, AP-ACl-3 exhibited considerable bioactivities significantly preventing the migration of MCF-7 cells and stimulating lymphocyte proliferation along with nitric oxide (NO) production of peritoneal macrophages. AP-ACl-3 could be explored as a novel potential anti-tumor and immunomodulatory agent.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China. zemilym87@yahoo.com.
authors.forename: Shaowei
authors.initials: S
authors.lastname: Wu
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China. roger1887@163.com.
authors.forename: Xiong
authors.initials: X
authors.lastname: Fu
authors.affiliation: Centre for Food Research and Innovation, Lincoln University, Lincoln 7648, New Zealand. Margaret.Brennan@Lincoln.ac.nz.
authors.forename: Margaret A
authors.initials: MA
authors.lastname: Brennan
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China. charles.brennan@lincoln.ac.nz.|Centre for Food Research and Innovation, Lincoln University, Lincoln 7648, New Zealand. charles.brennan@lincoln.ac.nz.
authors.forename: Charles S
authors.initials: CS
authors.lastname: Brennan
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China. cd0328@163.com.
authors.forename: Chen
authors.initials: C
authors.lastname: Chun
mesh_terms: D029912:Abrus / Q000737:chemistry*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D007155:Immunologic Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects; D008297:Male; D008807:Mice, Inbred BALB C; D009369:Neoplasms / Q000188:drug therapy; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D007155:Immunologic Factors; D011134:Polysaccharides
keywords: Abrus cantoniensis; anti-tumor; immunomodulatory; polysaccharide; purifying method