title: A new approach to synthesis of benzyl cinnamate: Optimization by response surface methodology.
abstract: In this work, the new approach to synthesis of benzyl cinnamate by enzymatic esterification of cinnamic acid with benzyl alcohol is optimized by response surface methodology. The effects of various reaction conditions, including temperature, enzyme loading, substrate molar ratio of benzyl alcohol to cinnamic acid, and reaction time, are investigated. A 5-level-4-factor central composite design is employed to search for the optimal yield of benzyl cinnamate. A quadratic polynomial regression model is used to analyze the experimental data at a 95% confidence level (P<0.05). The coefficient of determination of this model is found to be 0.9851. Three sets of optimum reaction conditions are established, and the verified experimental trials are performed for validating the optimum points. Under the optimum conditions (40°C, 31mg/mL enzyme loading, 2.6:1 molar ratio, 27h), the yield reaches 97.7%, which provides an efficient processes for industrial production of benzyl cinnamate.
authors.affiliation: College of Pharmaceutical Science, Hebei University, Baoding 071002, China; Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Science, Hebei University, Baoding 071002, China. Electronic address: donghaozhang1@163.com.
authors.forename: Dong-Hao
authors.initials: DH
authors.lastname: Zhang
authors.affiliation: College of Pharmaceutical Science, Hebei University, Baoding 071002, China.
authors.forename: Jiang-Yan
authors.initials: JY
authors.lastname: Zhang
authors.affiliation: Xiaowangguozhuang Township Hospital of Gaoyang County, Baoding 071500, China.
authors.forename: Wen-Cai
authors.initials: WC
authors.lastname: Che
authors.affiliation: College of Pharmaceutical Science, Hebei University, Baoding 071002, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
mesh_terms: D019905:Benzyl Alcohol / Q000378:metabolism*; D001593:Benzyl Compounds / Q000378:metabolism*; D002934:Cinnamates / Q000378:metabolism*; D004951:Esterification; D008049:Lipase / Q000378:metabolism*; D013696:Temperature
chemical_list: D001593:Benzyl Compounds; D002934:Cinnamates; C029010:cinnamic acid; D008049:Lipase; D019905:Benzyl Alcohol; C526327:benzyl cinnamate
keywords: Benzyl cinnamate; Esterification; Lipase; Optimization; Response surface methodology