title: Chemical imaging of molecular changes in a hydrated single cell by dynamic secondary ion mass spectrometry and super-resolution microscopy.
abstract: Chemical imaging of single cells at the molecular level is important in capturing biological dynamics. Single cell correlative imaging is realized between super-resolution microscopy, namely, structured illumination microscopy (SIM), and time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a multimodal microreactor (i.e., System for Analysis at the Liquid Vacuum Interface, SALVI). SIM characterized cells and guided subsequent ToF-SIMS analysis. Lipid fragments were identified in the cell membrane via dynamic ToF-SIMS depth profiling. Positive SIMS spectra show intracellular potassium and sodium ion transport due to exposure to nanoparticles. Spectral principal component analysis elucidates differences in chemical composition among healthy alveolar epithelial mouse lung C10 cells, cells that uptake zinc oxide nanoparticles, and various wet and dry control samples. The observation of Zn(+) gives the first direct evidence of ZnO NP uptake and dissolution by the cell membrane. Our results provide submicron chemical mapping for investigating cell dynamics at the molecular level.
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Nanjing, Jiangsu Province 211189, China. liusq@seu.edu.cn.
authors.forename: Xin
authors.initials: X
authors.lastname: Hua
authors.forename: Craig
authors.initials: C
authors.lastname: Szymanski
authors.forename: Zhaoying
authors.initials: Z
authors.lastname: Wang
authors.forename: Yufan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Xiang
authors.initials: X
authors.lastname: Ma
authors.forename: Jiachao
authors.initials: J
authors.lastname: Yu
authors.forename: James
authors.initials: J
authors.lastname: Evans
authors.forename: Galya
authors.initials: G
authors.lastname: Orr
authors.forename: Songqin
authors.initials: S
authors.lastname: Liu
authors.forename: Zihua
authors.initials: Z
authors.lastname: Zhu
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Yu
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry*; D002469:Cell Separation; D004847:Epithelial Cells / Q000737:chemistry* / Q000166:cytology*; D007089:Image Enhancement; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008853:Microscopy; D057054:Molecular Imaging / Q000379:methods*; D018629:Spectrometry, Mass, Secondary Ion; D014867:Water / Q000378:metabolism; D015034:Zinc Oxide / Q000737:chemistry
chemical_list: D014867:Water; D015034:Zinc Oxide