title: Two host microRNAs influence WSSV replication via STAT gene regulation.
abstract: MicroRNAs (miRNAs) have important roles in post-transcriptional regulation of gene expression. During viral infection, viruses utilize hosts to enhance their replication by altering cellular miRNAs. The Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway plays crucial roles in the antiviral responses. In this study, two miRNAs (miR-9041 and miR-9850) from Macrobrachium rosenbergii were found to promote white spot syndrome virus (WSSV) replication. The up-regulation of miR-9041 or miR-9850 suppresses STAT expression in the gills of M. rosenbergii, which subsequently down-regulates the expression of its downstream dynamin (Dnm) genes: Dnm1, Dnm2, and Dnm3. Knockdown of miR-9041 and miR-9850 restricts WSSV replication by up-regulating STAT and Dnm gene expression. The silencing of STAT, Dnm1, Dnm2, or Dnm3 led to an increase of the number of WSSV copies in shrimp. The injection of recombinant Dnm1, Dnm2, or Dnm3 proteins could inhibit WSSV replication in vivo. Overall, our research indicates the roles of host miRNAs in the enhancement of WSSV replication by regulating the host JAK/STAT pathway.
authors.affiliation: Jiangsu Key Laboratory for Biodiversity &Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Jiangsu Key Laboratory for Biodiversity &Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Wang
authors.affiliation: Jiangsu Key Laboratory for Biodiversity &Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Ren
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics* / Q000378:metabolism; D034281:Dynamins / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D053612:Janus Kinases / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D009843:Oligoribonucleotides / Q000235:genetics / Q000378:metabolism; D033541:Palaemonidae / Q000235:genetics* / Q000821:virology; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D050791:STAT Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D014779:Virus Replication / Q000187:drug effects; D046848:White spot syndrome virus 1 / Q000502:physiology*
chemical_list: D060829:Arthropod Proteins; D035683:MicroRNAs; D009843:Oligoribonucleotides; D020033:Protein Isoforms; D011994:Recombinant Proteins; D050791:STAT Transcription Factors; D053612:Janus Kinases; D034281:Dynamins