title: Anti-wrinkle effects of Seungma-Galgeun-Tang as evidenced by the inhibition of matrix metalloproteinase-I production and the promotion of type-1 procollagen synthesis.
abstract: BACKGROUND
  Seungma-Galgeun-Tang (SMGGT), a traditional herbal medicinal formula, has been used to treat various skin problems such as inflammation and rashes in Korean traditional medicine. In order to clarify the scientific evidence for the biological efficacy of SMGGT on the prevention of skin aging and in particular wrinkle formation, molecular anti-wrinkle parameters were evaluated in cultured human dermal fibroblasts.
  
  
  METHODS
  Standard SMGGT was prepared from KFDA-certified herbal medicines and the chemical fingerprint of SMGGT was verified by HPLC-ESI-MS to insure the quality of SMGGT. To evaluate the inhibitory effects of SMGGT on the synthesis of matrix metalloproteinase-1 (MMP-1) and type-1 procollagen, the content of MMP-1 and type-1 procollagen synthesizing enzymes in cultured human dermal fibroblasts were measured using an ELISA kit and Western Blot, respectively.
  
  
  RESULTS
  The treatment of SMGGT water extract significantly inhibited the production of MMP-1 and promoted type-1 procollagen synthesis concentration dependently.
  
  
  CONCLUSIONS
  These results suggest that SMGGT has the potential to prevent wrinkle formation by down-regulating MMP-1 and up-regulating type-1 procollagen in human dermal fibroblasts.
authors.affiliation: Division of Pharmacognosy, College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, South Korea.|Department of Life and Nanopharmaceutical Sciences, College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, South Korea.
authors.forename: Min Kyoung
authors.initials: MK
authors.lastname: Kim
authors.affiliation: Department of Preventive Pharmacy and Toxicology, College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, South Korea.
authors.forename: Chae Young
authors.initials: CY
authors.lastname: Bang
authors.affiliation: Division of Pharmacognosy, College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, South Korea.
authors.forename: Gwang Jun
authors.initials: GJ
authors.lastname: Yun
authors.affiliation: Department of Life and Nanopharmaceutical Sciences, College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, South Korea.
authors.forename: Hyang-Yu
authors.initials: HY
authors.lastname: Kim
authors.affiliation: Division of Pharmacognosy, College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, South Korea. ypjang@khu.ac.kr.|Department of Life and Nanopharmaceutical Sciences, College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, South Korea. ypjang@khu.ac.kr.
authors.forename: Young Pyo
authors.initials: YP
authors.lastname: Jang
authors.affiliation: Department of Preventive Pharmacy and Toxicology, College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, South Korea. sychoung@khu.ac.kr.|Department of Life and Nanopharmaceutical Sciences, College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, South Korea. sychoung@khu.ac.kr.
authors.forename: Se Young
authors.initials: SY
authors.lastname: Choung
mesh_terms: D002470:Cell Survival; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism*; D061965:Matrix Metalloproteinase Inhibitors / Q000494:pharmacology*; D011347:Procollagen / Q000096:biosynthesis*; D015595:Skin Aging / Q000187:drug effects*; D055815:Young Adult
chemical_list: D004365:Drugs, Chinese Herbal; D061965:Matrix Metalloproteinase Inhibitors; D011347:Procollagen; C000611873:seungma-galgeun-tang; D020781:Matrix Metalloproteinase 1
keywords: Anti-wrinkle; Matrix metalloproteinase-1; Seungma-Galgeun-Tang; Type-1 procollagen