title: The angiogenesis in decellularized scaffold-mediated the renal regeneration.
abstract: There are increasing numbers of patients underwent partial nephrectomy, and recovery of disturbed renal function is imperative post partial nephrectomy. We previously have demonstrated the decellularized (DC) scaffolds could mediate the residual kidney regeneration and thus improve disturbed renal function after partial nephrectomy. However, the cellular changes including the angiogenesis in the implanted DC scaffold has not yet been elaborated. In this study, we observed that the scaffold promoted the proliferation of human umbilical vein endothelial cells (HUVEC) that adhered to the DC scaffold in vitro. We next examined the pathological changes of the implanted DC graft in vivo, and found a decreased volume of the scaffold and a dramatic angiogenesis within the scaffold. The average microvessel density (aMVD) increased at the early stage, while decreased at the later stage post transplantation. Expression level of vascular endothelial growth factor (VEGF) showed similar dynamic changes. In addition, many endothelial cells (ECs) and endothelial progenitor cells (EPCs) were distributed in the region which contained active angiogenesis in the scaffold. However, the implanted graft became fibrosis and the angiogenesis degraded at final stage roughly 8 weeks post transplantation. Our data indicate that DC scaffold can be vascularized in vivo and possible mechanisms are discussed.
authors.affiliation: Anatomy Department, Wenzhou Medical University, Wenzhou, 325035, China.|Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, 325035, China.|Institute of Neuroscience, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Mei
authors.affiliation: Anatomy Department, Wenzhou Medical University, Wenzhou, 325035, China.|Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Medical School of Ningbo University, Ningbo, 315211, China.
authors.forename: Miaozhong
authors.initials: M
authors.lastname: Li
authors.affiliation: Anatomy Department, Wenzhou Medical University, Wenzhou, 325035, China.|Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Xi
authors.affiliation: Medical School of Ningbo University, Ningbo, 315211, China.
authors.forename: Sixiao
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Anatomy Department, Wenzhou Medical University, Wenzhou, 325035, China.|Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Anatomy Department, Wenzhou Medical University, Wenzhou, 325035, China.|Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Junqun
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Anatomy Department, Wenzhou Medical University, Wenzhou, 325035, China.|Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Jianse
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Neuroscience, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Yuqiang
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Xinfa
authors.initials: X
authors.lastname: Lou
authors.affiliation: Anatomy Department, Wenzhou Medical University, Wenzhou, 325035, China.|Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Maolin
authors.initials: M
authors.lastname: Tang
mesh_terms: D000071916:AC133 Antigen / Q000378:metabolism; D000818:Animals; D001808:Blood Vessels / Q000378:metabolism / Q000503:physiopathology; D017690:Cell Transplantation / Q000379:methods; D002478:Cells, Cultured; D061307:Human Umbilical Vein Endothelial Cells / Q000502:physiology* / Q000637:transplantation; D006801:Humans; D007668:Kidney / Q000098:blood supply / Q000503:physiopathology* / Q000601:surgery; D018919:Neovascularization, Physiologic / Q000502:physiology*; D009392:Nephrectomy / Q000379:methods; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D014183:Transplantation, Heterologous; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D000071916:AC133 Antigen; D042461:Vascular Endothelial Growth Factor A
keywords: angiogenesis; decellularized scaffolds; endothelial cells; endothelial progenitor cells; kidney regeneration