title: Tenascin-C promotes migration of hepatic stellate cells and production of type I collagen.
abstract: Tenascin-C (TN-C) is an extracellular matrix glycoprotein markedly upregulated during liver fibrosis. The study is performed to explore the role of TN-C during the growth and activation of hepatic stellate cells (HSCs). We found that TN-C was accumulated accompanying with the HSC activation. Our data on cell migration assay revealed that the rTN-C treatment enhanced HSC migration in a dose- and time-dependent manner, but did not influence their proliferation. HSCs transfected with pTARGET-TN-C overexpression vector displayed increased the type I collagen (Col I) production. TN-C overexpression enhanced the process of HSC activation through TGF-β1 signaling. Moreover, the anti-α9β1 integrin antibody treatment blocked the TN-C-driven Col I increase in rat HSCs. Collectively, TN-C had a positive role in activation of HSCs mediated by TGF-β1 and α9β1 integrin, manifesting elevation of Col I production and promotion of cell migration. Our results provide a potential insight for the therapy of hepatic fibrosis.
authors.affiliation: a Department of General Surgery , Second Affiliated Hospital of Xi'an Jiaotong University , Xi'an , China.
authors.forename: Jian-Cang
authors.initials: JC
authors.lastname: Ma
authors.affiliation: b Department of General Surgery , Xi'an Central Hospital, Xi'an Jiaotong University , Xi'an , China.
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.affiliation: b Department of General Surgery , Xi'an Central Hospital, Xi'an Jiaotong University , Xi'an , China.
authors.forename: Ya-Wei
authors.initials: YW
authors.lastname: Shen
authors.affiliation: b Department of General Surgery , Xi'an Central Hospital, Xi'an Jiaotong University , Xi'an , China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zheng
authors.affiliation: a Department of General Surgery , Second Affiliated Hospital of Xi'an Jiaotong University , Xi'an , China.
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Su
authors.affiliation: a Department of General Surgery , Second Affiliated Hospital of Xi'an Jiaotong University , Xi'an , China.
authors.forename: Jin-Kai
authors.initials: JK
authors.lastname: Xu
authors.affiliation: a Department of General Surgery , Second Affiliated Hospital of Xi'an Jiaotong University , Xi'an , China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D024042:Collagen Type I / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D005822:Genetic Vectors / Q000737:chemistry / Q000235:genetics; D055166:Hepatic Stellate Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D016023:Integrins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008103:Liver Cirrhosis / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008297:Male; D008954:Models, Biological; D061251:Primary Cell Culture; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction; D019063:Tenascin / Q000494:pharmacology*; D014162:Transfection; D053773:Transforming Growth Factor beta1 / Q000235:genetics* / Q000378:metabolism
chemical_list: D000906:Antibodies; D024042:Collagen Type I; D016023:Integrins; D011994:Recombinant Proteins; D019063:Tenascin; D053773:Transforming Growth Factor beta1; C113256:integrin alpha 9 beta 1
keywords: TGF-β1; hepatic stellate cell; tenascin-C(TN-C); type I collagen(Col I); α9β1 integrin