title: Angiotensin I-converting enzyme (ACE) inhibitory activity and structural properties of oven- and freeze-dried protein hydrolysate from fresh water fish (Cirrhinus mrigala).
abstract: The angiotensin I-converting enzyme (ACE) inhibitory activity and structural properties of oven-dried (OD-FPH) and freeze-dried (FD-FPH) protein hydrolysates derived from fresh water fish (Cirrhinus mrigala) muscle, using papain, were investigated. Amino acid profiles indicated a higher proportion of hydrophobic residues in OD-FPH and hydrophilic residues in FD-FPH samples. Fourier transform infrared (FT-IR) spectra revealed random coil structure in OD-FPH and β-sheet in FD-FPH samples. The approximate molecular weight of peptides in OD-FPH and FD-FPH was in the range of 7030-339Da. The IC50 values for ACE inhibition by OD-FPH and FD-FPH samples were found to be 1.15 and 1.53mg of proteinml(-1), respectively. The ACE-inhibitory activity of OD-FPH was more stable (during sequential digestion, using pepsin and pancreatin) than that of FD-FPH sample. The study suggested that the ACE inhibitory activity of protein hydrolysate was not affected by oven-drying.
authors.affiliation: Department of Fish Processing Technology, Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar, College of Fisheries, Mangalore 575 002, India.
authors.forename: K
authors.initials: K
authors.lastname: Elavarasan
authors.affiliation: Department of Fish Processing Technology, Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar, College of Fisheries, Mangalore 575 002, India. Electronic address: bashamasundar@rediffmail.com.
authors.forename: B A
authors.initials: BA
authors.lastname: Shamasundar
authors.affiliation: Department of Nutrition and Food Safety, Faculty of Health and Medical Sciences, University of Surrey, Guildford GU2 7XH, UK.
authors.forename: Faraha
authors.initials: F
authors.lastname: Badii
authors.affiliation: Department of Nutrition and Food Safety, Faculty of Health and Medical Sciences, University of Surrey, Guildford GU2 7XH, UK.
authors.forename: Nazlin
authors.initials: N
authors.lastname: Howell
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry*; D000818:Animals; D003530:Cyprinidae; D005511:Food Handling / Q000379:methods*; D005612:Freeze Drying; D005618:Fresh Water; D020128:Inhibitory Concentration 50; D008970:Molecular Weight; D010194:Pancreatin / Q000737:chemistry; D010206:Papain / Q000737:chemistry; D010434:Pepsin A / Q000737:chemistry; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D011492:Protein Hydrolysates / Q000737:chemistry*; D017747:Seafood / Q000032:analysis*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000596:Amino Acids; D000806:Angiotensin-Converting Enzyme Inhibitors; D011492:Protein Hydrolysates; D010194:Pancreatin; D007703:Peptidyl-Dipeptidase A; D010206:Papain; D010434:Pepsin A
keywords: ACE-inhibitory activity; Bioactive peptide; FT-IR spectra; Mrigal protein hydrolysate; Oven-drying