title: Proteomic Changes in Chicken Plasma Induced by Salmonella typhimurium Lipopolysaccharides.
abstract: Lipopolysaccharides (LPS) are cell wall components of Gram-negative bacteria that produce inflammation and sickness in higher animals. The objective was to identify plasma proteomic changes in an avian model of inflammation. Chickens were treated with either saline or LPS, and blood was collected at 24 hours postinjection. The pooled plasma samples were depleted of high-abundant proteins and analyzed by matrix-assisted laser desorption ionization (MALDI)-time-of-flight mass spectrometry and liquid chromatography-tandem mass spectrometry (LC-MS/MS). MALDI analyses showed an increase in fibrinogen beta-derived peptide and a decrease in apolipoprotein-AII-derived peptide in LPS samples. Label-free quantitation of LC-MS/MS spectra revealed an increase in the levels of α1-acid glycoprotein, a chemokine CCLI10, and cathelicidin-2, but a decrease in an interferon-stimulated gene-12-2 protein in the LPS group. These differentially expressed proteins are associated with immunomodulation, cytokine changes, and defense mechanisms, which may be useful as candidate biomarkers of infection and inflammation.
authors.affiliation: Cell and Molecular Biology Program, University of Arkansas, Fayetteville, AR, USA.; Department of Poultry Science, University of Arkansas, Fayetteville, AR, USA.; Poultry Production and Product Safety Research Unit, Agricultural Research Service, USDA, Poultry Science Center, University of Arkansas, Fayetteville, AR, USA.
authors.forename: Balamurugan
authors.initials: B
authors.lastname: Packialakshmi
authors.affiliation: Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR, USA.
authors.forename: Rohana
authors.initials: R
authors.lastname: Liyanage
authors.affiliation: Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR, USA.
authors.forename: Jackson O
authors.initials: JO
authors.lastname: Lay
authors.affiliation: Department of Poultry Science, University of Arkansas, Fayetteville, AR, USA.; Poultry Production and Product Safety Research Unit, Agricultural Research Service, USDA, Poultry Science Center, University of Arkansas, Fayetteville, AR, USA.
authors.forename: Sarbjeet K
authors.initials: SK
authors.lastname: Makkar
authors.affiliation: Poultry Production and Product Safety Research Unit, Agricultural Research Service, USDA, Poultry Science Center, University of Arkansas, Fayetteville, AR, USA.
authors.forename: Narayan C
authors.initials: NC
authors.lastname: Rath
keywords: acute-phase proteins; biomarkers; endotoxin; innate immunity; proteomics