title: Effects of RGD immobilization on light-induced cell sheet detachment from TiO2 nanodots films.
abstract: Light-induced cell detachment is reported to be a safe and effective cell sheet harvest method. In the present study, the effects of arginine-glycine-aspartic acid (RGD) immobilization on cell growth, cell sheet construction and cell harvest through light illumination are investigated. RGD was first immobilized on TiO2 nanodots films through simple physical adsorption, and then mouse pre-osteoblastic MC3T3-E1 cells were seeded on the films. It was found that RGD immobilization promoted cell adhesion and proliferation. It was also observed that cells cultured on RGD immobilized films showed relatively high level of pan-cadherin. Cells harvested with ultraviolet illumination (365 nm) showed good viability on both RGD immobilized and unmodified TiO2 nanodot films. Single cell detachment assay showed that cells detached more quickly on RGD immobilized TiO2 nanodot films. That could be ascribed to the RGD release after UV365 illumination. The current study demonstrated that RGD immobilization could effectively improve both the cellular responses and light-induced cell harvest.
authors.affiliation: School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou 310027, China.
authors.forename: Kui
authors.initials: K
authors.lastname: Cheng
authors.affiliation: School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou 310027, China.
authors.forename: Tiantian
authors.initials: T
authors.lastname: Wang
authors.affiliation: The Affiliated Stomatologic Hospital, Zhejiang University, Hangzhou 310003, China; The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou, 310003, China.
authors.forename: Mengliu
authors.initials: M
authors.lastname: Yu
authors.affiliation: School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou 310027, China.
authors.forename: Hongping
authors.initials: H
authors.lastname: Wan
authors.affiliation: The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou, 310003, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lin
authors.affiliation: School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou 310027, China; The Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, China. Electronic address: wengwj@zju.edu.cn.
authors.forename: Wenjian
authors.initials: W
authors.lastname: Weng
authors.affiliation: The Affiliated Stomatologic Hospital, Zhejiang University, Hangzhou 310003, China; The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou, 310003, China. Electronic address: hmwang1960@hotmail.com.
authors.forename: Huiming
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects / Q000528:radiation effects; D018929:Cell Culture Techniques / Q000295:instrumentation; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D051379:Mice; D018613:Microscopy, Confocal; D049329:Nanostructures / Q000737:chemistry* / Q000633:toxicity; D009842:Oligopeptides / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D015820:Cadherins; D009842:Oligopeptides; C009495:titanium dioxide; C047981:arginyl-glycyl-aspartic acid; D014025:Titanium
keywords: Cell detachment; Nanodots film; RGD immobilization; TiO(2); UV365 illumination