title: Interaction Mode between Inclusion Complex of Vitamin K3 with γ- Cyclodextrin and Herring-Sperm DNA.
abstract: Methods including spectroscopy, electronic chemistry and thermodynamics were used to study the inclusion effect between γ-cyclodextrin (CD) and vitamin K3(K3), as well as the interaction mode between herring-sperm DNA (hsDNA) and γ-CD-K3 inclusion complex. The results from ultraviolet spectroscopic method indicated that VK3 and γ-CD formed 1:1 inclusion complex, with the inclusion constant Kf = 1.02 × 10(4) L/mol, which is based on Benesi-Hildebrand's viewpoint. The outcomes from the probe method and Scatchard methods suggested that the interaction mode between γ-CD-K3 and DNA was a mixture mode, which included intercalation and electrostatic binding effects. The binding constants were K (θ)25°C = 2.16 × 10(4) L/mol, and K(θ)37°C = 1.06 × 10(4) L/mol. The thermodynamic functions of the interaction between γ-CD-K3 and DNA were ΔrHm(θ) = -2.74 × 10(4) J/mol, ΔrSm(θ) = 174.74 J·mol(-1)K(-1), therefore, both ΔrHm(θ) (enthalpy) and ΔrSm(θ) (entropy) worked as driven forces in this action.
authors.affiliation: a College of Chemistry and Materials Science , Sichuan Normal University , Chengdu , P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Tang
authors.affiliation: a College of Chemistry and Materials Science , Sichuan Normal University , Chengdu , P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Cai
authors.affiliation: a College of Chemistry and Materials Science , Sichuan Normal University , Chengdu , P.R. China.
authors.forename: Kang
authors.initials: K
authors.lastname: Xue
authors.affiliation: b College of Chemical Engineering , Sichuan University , Chengdu , P.R. China.
authors.forename: Chunling
authors.initials: C
authors.lastname: Wang
authors.affiliation: a College of Chemistry and Materials Science , Sichuan Normal University , Chengdu , P.R. China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Xiong
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry*; D005399:Fishes / Q000235:genetics; D007364:Intercalating Agents / Q000737:chemistry*; D008297:Male; D033362:Powder Diffraction; D013094:Spermatozoa / Q000737:chemistry; D013816:Thermodynamics; D024483:Vitamin K 3 / Q000737:chemistry*; D014961:X-Ray Diffraction; D047408:gamma-Cyclodextrins / Q000737:chemistry*
chemical_list: D007364:Intercalating Agents; D047408:gamma-Cyclodextrins; D024483:Vitamin K 3; D004247:DNA; C023792:gamma-cyclodextrin
keywords: Vitamin K3; herring-sperm DNA; molecular probe; γ-cyclodextrin