title: Treatment of experimental furcation perforations with mineral trioxide aggregate, platelet rich plasma or platelet rich fibrin in dogs' teeth.
abstract: This work evaluates the effect of mineral trioxide aggregate (MTA), platelet rich plasma (PRP) or platelet rich fibrin (PRF) on healing of non-contaminated and contaminated furcation perforations. A total of 192 teeth of 12 dogs was divided into three equal groups according to evaluation period. Each group was further subdivided into MTA, PRP, PRF, negative and positive control subgroups. Each experimental subgroup was further subdivided according to perforation status into non-contaminated and contaminated subdivisions. Root canal therapy was carried out and furcation perforation was made in all teeth except in negative control subgroup. The furcation perforation was repaired immediately in subdivision (1) and after 4 weeks in subdivision (2). The change in vertical bone loss was measured by radiography. Inflammatory cell count, cemental deposition, new bone formation, bone resorption and epithelial proliferation were assessed. Both PRP and PRF demonstrated statistically significant reduction in vertical bone loss and inflammatory cell count than MTA. No significant difference was found between MTA, PRP and PRF in cemental deposition, new bone formation, bone resorption and epithelial proliferation. The non-contaminated teeth demonstrated better treatment outcomes than the contaminated teeth. In conclusion, PRP and PRF are successful treatment options for repairing of furcation perforation in both non-contaminated and contaminated teeth in dogs with superior outcomes in non contaminated teeth.
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Ain Shams University, Cairo, Egypt.
authors.forename: Hosam E
authors.initials: HE
authors.lastname: Tawfik
authors.affiliation: Department of Surgery, Anesthesiology & Radiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt. Electronic address: ashrafseida@cu.edu.eg.
authors.forename: Ashraf M
authors.initials: AM
authors.lastname: Abu-Seida
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Ain Shams University, Cairo, Egypt.
authors.forename: Ahmed A
authors.initials: AA
authors.lastname: Hashem
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Ain Shams University, Cairo, Egypt.
authors.forename: Mohammed M
authors.initials: MM
authors.lastname: El-Khawlani
mesh_terms: D017607:Aluminum Compounds / Q000494:pharmacology*; D000818:Animals; D017610:Calcium Compounds / Q000494:pharmacology*; D004195:Disease Models, Animal; D004285:Dogs; D004338:Drug Combinations; D005337:Fibrin / Q000494:pharmacology*; D010087:Oxides / Q000494:pharmacology*; D053657:Platelet-Rich Plasma; D012387:Root Canal Filling Materials / Q000494:pharmacology; D012390:Root Canal Therapy / Q000009:adverse effects; D017640:Silicates / Q000494:pharmacology*; D014092:Tooth Root / Q000187:drug effects / Q000293:injuries*
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; D005337:Fibrin
keywords: Cemental deposition; Dogs; Furcation perforation; MTA; PRF; PRP