title: Quantification of the environmental neurotoxin annonacin in Rat brain by UPLC-MS/MS.
abstract: The Annonaceous acetogenin annonacin is an environmental neurotoxin identified in the pulp of several fruits of the Annonaceae family, whose consumption was linked to the occurrence of sporadic atypical Parkinsonism with dementia. A method for its quantification in Rat brain homogenates by UPLC-MS/MS in selected reaction monitoring (SRM) mode was developed and validated. This method was applied to the quantitation of annonacin in Rat brain after intravenous (0.5 mg/kg) and oral (10 mg/kg, 100 mg/kg) administration.
authors.affiliation: Laboratoire de Pharmacognosie, BioCIS, Univ. Paris-Sud, CNRS, Université Paris-Saclay, UFR Pharmacie, 5 rue J.-B. Clément, 92290, Châtenay-Malabry, France.
authors.forename: Natacha
authors.initials: N
authors.lastname: Bonneau
authors.affiliation: Institut de Chimie des Substances Naturelles, CNRS UPR2301, Université Paris-Saclay, Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Schmitz-Afonso
authors.affiliation: Institut de Chimie des Substances Naturelles, CNRS UPR2301, Université Paris-Saclay, Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Brunelle
authors.affiliation: Institut de Chimie des Substances Naturelles, CNRS UPR2301, Université Paris-Saclay, Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France.
authors.forename: David
authors.initials: D
authors.lastname: Touboul
authors.affiliation: Laboratoire de Pharmacognosie, BioCIS, Univ. Paris-Sud, CNRS, Université Paris-Saclay, UFR Pharmacie, 5 rue J.-B. Clément, 92290, Châtenay-Malabry, France. Electronic address: pierre.champy@u-psud.fr.
authors.forename: Pierre
authors.initials: P
authors.lastname: Champy
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001711:Biotransformation; D001812:Blood-Brain Barrier / Q000187:drug effects / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D042967:Electron Transport Complex I / Q000037:antagonists & inhibitors / Q000378:metabolism; D004785:Environmental Pollutants / Q000008:administration & dosage / Q000032:analysis / Q000633:toxicity*; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000032:analysis / Q000633:toxicity; D005663:Furans / Q000008:administration & dosage / Q000032:analysis / Q000633:toxicity*; D007275:Injections, Intravenous; D007783:Lactones / Q000008:administration & dosage / Q000032:analysis / Q000633:toxicity*; D008297:Male; D009474:Neurons / Q000737:chemistry / Q000378:metabolism*; D017208:Rats, Wistar; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D014018:Tissue Distribution; D066007:Toxicokinetics; D014118:Toxins, Biological / Q000008:administration & dosage / Q000032:analysis / Q000633:toxicity*
chemical_list: D004785:Environmental Pollutants; D004791:Enzyme Inhibitors; D005663:Furans; D007783:Lactones; D014118:Toxins, Biological; C053442:annonacin; D042967:Electron Transport Complex I
keywords: Acetogenin; Annonaceae; Annonacin; Distribution; Rat brain; UPLC-MS/MS