title: Protective effect of berberine on doxorubicin‑induced acute hepatorenal toxicity in rats.
abstract: Doxorubicin (DOX), a potent broad‑spectrum chemotherapeutic agent used for the treatment of several types of cancer, is largely limited due to its serious side effects on non‑target organs. Thus, the present study aimed to investigate whether berberine (Ber), an isoquinoline alkaloid, could reduce DOX‑induced acute hepatorenal toxicity in rats. Fifty rats were randomly divided into five groups: i) Control group, ii) DOX group, iii) DOX+Ber (5 mg kg) group; iv) DOX+Ber (10 mg kg), and v) DOX+Ber (20 mg kg) group. In the tests, body weight, organ index, general condition and mortality were observed. In addition, the serum levels of alanine transaminase (ALT), aspartate aminotransferase (AST), total cholesterol (TCHO) and blood urea nitrogen (BUN) were determined to evaluate hepatorenal function. Hepatorenal toxicity was further assessed using hematoxylin and eosin stained sections. Furthermore, the levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and malondialdehyde (MDA) in rat serum or tissue homogenate were also assessed to determine the mechanisms of action. Results suggested that pretreatment with Ber ameliorated the DOX‑induced liver and kidney injury by lowering the serum ALT, AST, TCHO and BUN levels, and the damage observed histologically, such as hemorrhage and focal necrosis of liver and kidney tissues induced by DOX were also attenuated by Ber. Furthermore, Ber also exerted certain antioxidative properties through reversing the changes in the levels of MDA, SOD, GSH and MDA induced by DOX. These findings indicate that Ber has protective effects against DOX‑induced acute hepatorenal toxicity in rats. Combination of Ber with DOX is a novel strategy that has the potential for protecting against DOX‑induced hepatorenal toxicity in clinical practice.
authors.affiliation: The Key Laboratory of Neural and Vascular Biology, Ministry of Education, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China.
authors.forename: Xueyan
authors.initials: X
authors.lastname: Chen
authors.affiliation: The Family Planning Research Institute of Hebei, Shijiazhuang, Hebei 050000, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Neural and Vascular Biology, Ministry of Education, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China.
authors.forename: Zhongning
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Pharmaceutical Department, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China.
authors.forename: Huanlong
authors.initials: H
authors.lastname: Liu
authors.affiliation: The Key Laboratory of Neural and Vascular Biology, Ministry of Education, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China.
authors.forename: Huicai
authors.initials: H
authors.lastname: Guo
authors.affiliation: The Key Laboratory of Neural and Vascular Biology, Ministry of Education, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Xiong
authors.affiliation: The Key Laboratory of Neural and Vascular Biology, Ministry of Education, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China.
authors.forename: Kerang
authors.initials: K
authors.lastname: Xie
authors.affiliation: The Key Laboratory of Neural and Vascular Biology, Ministry of Education, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Neural and Vascular Biology, Ministry of Education, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China.
authors.forename: Suwen
authors.initials: S
authors.lastname: Su
mesh_terms: D000818:Animals; D001599:Berberine / Q000494:pharmacology*; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000517:prevention & control; D004317:Doxorubicin / Q000009:adverse effects* / Q000494:pharmacology; D007674:Kidney Diseases / Q000097:blood / Q000139:chemically induced / Q000517:prevention & control; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001599:Berberine; D004317:Doxorubicin