title: Immune Response Induced by an Immunodominant 60 kDa Glycoprotein of the Cell Wall of Sporothrix schenckii in Two Mice Strains with Experimental Sporotrichosis.
abstract: Cell wall (CW) components of fungus Sporothrix schenckii are the major inductors antigens of immune responses. The immunodominant 60 kDa glycoprotein (gp60) has been shown to be associated with the virulence of this fungus but its role in experimental sporotrichosis is unknown. In this work, the immunological effects of CW-purified gp60 were investigated in a model of experimental subcutaneous sporotrichosis in normal and gp60-preimmunized C57BL/6 and BALB/c mice strains which were then infected with S. schenckii conidia. Results showed that both mice strains use different cytokine profiles in order to fight S. schenckii infection; C57BL/6 mice seem to use a Th17 response while BALB/c mice tend to depend on a Th1 profile. Preimmunization with gp60 showed a downregulatory effect on the immune response since cytokines levels were diminished in both strains. There were no significant differences in the magnitude of dorsoplantar inflammation between gp60-preimmunized and nonimmunized mice of both strains. However, skin lesions due to the infection in gp60-preimmunized mice were more severe in BALB/c than in C57BL/6 mice, suggesting that the antigen exerts a higher downregulatory effect on the Th1 response.
authors.affiliation: Facultad de Ciencias Químicas, Universidad Juárez del Estado de Durango, Avenida Veterinaria S/N, 34120 Durango, DGO, Mexico.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Alba-Fierro
authors.affiliation: Departamento de Biología Celular y Tisular, Facultad de Medicina, Universidad Nacional Autónoma de México, 04510 Ciudad de México, DF, Mexico.
authors.forename: Armando
authors.initials: A
authors.lastname: Pérez-Torres
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, 04510 Ciudad de México, DF, Mexico.
authors.forename: Conchita
authors.initials: C
authors.lastname: Toriello
authors.affiliation: Departamento de Biología Celular y Tisular, Facultad de Medicina, Universidad Nacional Autónoma de México, 04510 Ciudad de México, DF, Mexico.
authors.forename: Evelyn
authors.initials: E
authors.lastname: Pulido-Camarillo
authors.affiliation: Departamento de Biología, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Campus Guanajuato, Noria Alta S/N, 36050 Guanajuato, GTO, Mexico.
authors.forename: Everardo
authors.identifier: 0000-0002-2016-7399
authors.initials: E
authors.lastname: López-Romero
authors.affiliation: Departamento de Microbiología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Avenida Universidad No. 940, 20131 Aguascalientes, AGS, Mexico.
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Romo-Lozano
authors.affiliation: Department of Biochemistry and Molecular Biology and Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA 30602-4712, USA.
authors.forename: Gerardo
authors.initials: G
authors.lastname: Gutiérrez-Sánchez
authors.affiliation: Facultad de Ciencias Químicas, Universidad Juárez del Estado de Durango, Avenida Veterinaria S/N, 34120 Durango, DGO, Mexico.
authors.forename: Estela
authors.identifier: 0000-0003-1621-3505
authors.initials: E
authors.lastname: Ruiz-Baca
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000946:Antigens, Fungal / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D002473:Cell Wall / Q000737:chemistry / Q000276:immunology*; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D015870:Gene Expression; D006023:Glycoproteins / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D007111:Immunity, Cellular / Q000187:drug effects*; D007114:Immunization; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D013045:Species Specificity; D013172:Spores, Fungal / Q000737:chemistry / Q000276:immunology / Q000472:pathogenicity; D013175:Sporothrix / Q000737:chemistry / Q000276:immunology* / Q000472:pathogenicity; D013174:Sporotrichosis / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D018417:Th1 Cells / Q000276:immunology / Q000382:microbiology; D058408:Th1-Th2 Balance; D058504:Th17 Cells / Q000276:immunology / Q000382:microbiology
chemical_list: D000946:Antigens, Fungal; D016207:Cytokines; D006023:Glycoproteins