title: In vivo imaging of cortical interneurons migrating in the intermediate/subventricular zones.
abstract: We developed an imaging system that enables migrating cortical interneurons (CIs) through the lower intermediate zone/subventricular zone (IZ/SVZ) in mouse embryos. CIs were labeled by in utero electroporation performed at embryonic day (E) 11.5 and were observed, through the skull of living embryos, detached from the dam with the umbilical cord remain attached. To identify imaged cell locations, we used GAD67-GFP mice and GFP fluorescence was photo-bleached after the recording. We found that CIs in the IZ/SVZ migrated medially straight toward the midline on the tangential plane, while those in the marginal zone migrated in all directions.
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University Yamadaoka 1-3, Suita, Osaka 565-0871, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Higuchi
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University Yamadaoka 1-3, Suita, Osaka 565-0871, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kita
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University Yamadaoka 1-3, Suita, Osaka 565-0871, Japan. Electronic address: murakami@fbs.osaka-u.ac.jp.
authors.forename: Fujio
authors.initials: F
authors.lastname: Murakami
mesh_terms: D000818:Animals; D002465:Cell Movement; D002540:Cerebral Cortex / Q000166:cytology*; D004625:Embryo, Nonmammalian; D005968:Glutamate Decarboxylase / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D007395:Interneurons / Q000502:physiology*; D020547:Lateral Ventricles / Q000166:cytology*; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D036641:Microscopy, Fluorescence, Multiphoton; D059906:Neuroimaging
chemical_list: D049452:Green Fluorescent Proteins; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1
keywords: Cortical interneuron; In utero electroporation; In vivo imaging; Migration; Mouse embryos