title: Nonionic surfactants induced changes in cell characteristics and phenanthrene degradation ability of Sphingomonas sp. GY2B.
abstract: Surfactant-mediated bioremediation has been widely applied in decontaminating PAH-polluted sites. However, the impacts of surfactants on the biodegradation of PAHs have been controversial in the past years. To gain a clear insight into the influencing mechanisms, three nonionic surfactants (Tween80, TritonX-100 and Brij30) were selected to systematically investigate their effects on cell surface properties (membrane permeability, functional groups and elements), cell vitality as well as subsequent phenanthrene degradation ability of Sphingomonas sp. GY2B. Results showed that biodegradation of phenanthrene was stimulated by Tween80, slightly inhibited by TritonX-100 and severely inhibited by Brij30, respectively. Positive effect of Tween80 may arise from its role as the additional carbon source for GY2B to increase bacterial growth and activity, as demonstrated by the increasing viable cells in Tween80 amended degradation systems determined by flow cytometry. Although TritonX-100 could inhibit bacterial growth and disrupt cell membrane, its adverse impacts on microbial cells were weaker than Brij30, which may result in its weaker inhibitive extent. Results from this study can provide a rational basis on selecting surfactants for enhancing bioremediation of PAHs.
authors.affiliation: School of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Liu
authors.affiliation: School of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China; The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou 510006, PR China. Electronic address: clguo@scut.edu.cn.
authors.forename: Chuling
authors.initials: C
authors.lastname: Guo
authors.affiliation: School of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China.
authors.forename: Xujun
authors.initials: X
authors.lastname: Liang
authors.affiliation: School of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China.
authors.forename: Fengji
authors.initials: F
authors.lastname: Wu
authors.affiliation: School of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China; The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou 510006, PR China. Electronic address: chzdang@scut.edu.cn.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Dang
mesh_terms: D001673:Biodegradation, Environmental; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D050296:Microbial Viability / Q000187:drug effects; D017830:Octoxynol / Q000737:chemistry; D010616:Phenanthrenes / Q000378:metabolism*; D000077423:Polidocanol; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry; D011136:Polysorbates / Q000737:chemistry; D020578:Sphingomonas / Q000187:drug effects* / Q000378:metabolism; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D011136:Polysorbates; D013501:Surface-Active Agents; D000077423:Polidocanol; D011092:Polyethylene Glycols; C031181:phenanthrene; D017830:Octoxynol
keywords: Biodegradation; Cell characteristics; Phenanthrene; Surfactant