title: Utility of a novel triple marker (combination of thyroid transcription factor 1, Napsin A, and P40) in the subclassification of non-small cell lung carcinomas using fine-needle aspiration cases.
abstract: Personalized treatment of lung cancer requires an accurate subclassification of non-small cell lung carcinoma (NSCLC) into adenocarcinoma (ADC), squamous cell carcinoma (SqCC), and other subtypes. In poorly differentiated tumors especially on small fine-needle aspirate specimens, the subclassification could be difficult in certain cases. Our previous study using resected tumor tissue has shown that the combination of commonly used individual markers (thyroid transcription factor 1 [TTF-1], P40, and Napsin A) into a novel triple marker has high sensitivity and specificity in subclassification of NSCLC and also the advantage of using minimal tumor tissue. In this study, we further evaluated the utility of this novel triple marker using fine-needle aspirate cases. We included primary NSCLC, consisting of 37 SqCCs (primary, 35; metastasis, 2) and 50 ADCs (primary, 29; metastasis, 21), 12 metastatic ADCs of nonpulmonary primary, and 10 small cell lung carcinomas. The immunohistochemical patterns were semiquantitatively scored. In lung SqCCs and ADCs, the sensitivity and specificity of the triple marker were 100% and 97.1% and 86.0% and 100%, respectively. The triple marker showed no immunoreactivity in 12 metastatic nonpulmonary ADCs. In 10 small cell lung carcinomas, TTF-1 had focal positivity in 40% of cases. The limitations of the triple marker include staining of alveolar macrophages (by TTF-1 and Napsin A), basal layer of bronchial epithelial cells (by P40), and nonspecific cytoplasmic staining of TTF-1. Our study not only supports our previous finding using resected tumor specimens but also provides evidence that the triple marker can be used for cytological material and preserving tumor tissue for molecular testing.
authors.affiliation: The Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21287. Electronic address: rsharma@jhmi.edu.
authors.forename: Rajni
authors.initials: R
authors.lastname: Sharma
authors.affiliation: The Department of Chemistry, Magdalen College, University of Oxford, Oxford OX1 4AU, United Kingdom.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21287.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: The Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21287.
authors.forename: Grzegorz T
authors.initials: GT
authors.lastname: Gurda
authors.affiliation: The Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21287.
authors.forename: Susan
authors.initials: S
authors.lastname: Geddes
authors.affiliation: The Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21287.
authors.forename: Edward
authors.initials: E
authors.lastname: Gabrielson
authors.affiliation: The Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21287.
authors.forename: Frederic
authors.initials: F
authors.lastname: Askin
authors.affiliation: The Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21287. Electronic address: qli23@jhmi.edu.
authors.forename: Qing Kay
authors.initials: QK
authors.lastname: Li
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D016282:Aspartic Acid Endopeptidases / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D044963:Biopsy, Fine-Needle; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry* / Q000145:classification / Q000556:secondary; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000737:chemistry* / Q000145:classification / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis*; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors / Q000032:analysis*; D025521:Tumor Suppressor Proteins / Q000032:analysis*
chemical_list: D014408:Biomarkers, Tumor; C000614412:NKX2-1 protein, human; D009687:Nuclear Proteins; D010446:Peptide Fragments; C115051:TP63 protein, human; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; D016282:Aspartic Acid Endopeptidases; C498549:NAPSA protein, human
keywords: Fine-needle aspiration (FNA) cytology; Immunohistochemical (IHC) marker; Non–small cell lung carcinoma (NSCLC); P40; TTF-1 and Napsin A