title: An Intravenous Bolus of Epa: Dha 6: 1 Protects Against Myocardial Ischemia-Reperfusion-Induced Shock.
abstract: Enriching the diet with Omega-3 for several weeks improves myocardial resistance to ischemia-reperfusion (IR) in rats. However, patients with myocardial infarction requiring an emergency reperfusion cannot be pretreated with such a diet. The objective of our study was to describe the effects of an intravenous Omega-3 bolus before reperfusion in a rat model of myocardial IR.
  
  
  
  In a rat model of acute myocardial IR, an intravenous Omega-3 bolus (EPA:DHA 6:1), associated or not with iodinated contrast media, was administered after a 30-min ischemia, before reperfusion. Hemodynamic parameters were assessed. Circulating procoagulant microparticles were phenotyped. Vascular and heart inflammation, superoxide anion, and nitric oxide were measured. Ex vivo vascular reactivity was performed with a pharmacological selective inhibitor of inductible nitric oxide synthase. Cardiac troponin I (cTn-I) plasma levels were measured.
  
  
  
  Compared with untreated IR rats, an Omega-3 bolus before reperfusion significantly decreased the IR syndrome, improving mean arterial pressure (114 ± 9 vs. 61 ± 17 mmHg 4 h after reperfusion, P < 0.05) and carotid blood flow, and decreasing plasma cTn-I levels after revascularization. These beneficial effects may be due to improved ex vivo mesenteric resistance artery sensitivity to phenylephrine, endothelial protection assessed by decreased endothelial CD54 microparticle release (9.1 ± 2.5 vs. 4.8 ± 2.0 nM Eq PhtdSer, P < 0.05) and reduced vascular inflammation and oxidative stress.
  
  
  
  In this rat model of myocardial IR, an intravenous Omega-3 bolus before reperfusion decreases IR-induced vascular failure and shock. These results open therapeutic perspectives as far as myocardial reperfusion process is concerned that deserve further explorations in humans.
authors.affiliation: *EA 7293 FMTS, University of Strasbourg, Strasbourg, France †Biophotonic anc Pharmacology Laboratory, UMR 7213, University of Strasbourg, Illkirch, France ‡EA 4278, University of Avignon, F84000 Avignon, France §Laboratory of Medical Biostatistics and Informatics, University of Strasbourg, Strasbourg, France ¶Group Method in Clinical Research, Public Health Unit, Strasbourg University Hospital, Strasbourg, France ||Medical Intensive Care Unit, Strasbourg University Hospital, Strasbourg, France.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Burban
authors.forename: Grégory
authors.initials: G
authors.lastname: Meyer
authors.forename: Anne
authors.initials: A
authors.lastname: Olland
authors.forename: François
authors.initials: F
authors.lastname: Séverac
authors.forename: Blandine
authors.initials: B
authors.lastname: Yver
authors.forename: Florence
authors.initials: F
authors.lastname: Toti
authors.forename: Valérie
authors.initials: V
authors.lastname: Schini-Kerth
authors.forename: Ferhat
authors.initials: F
authors.lastname: Meziani
authors.forename: Julie
authors.initials: J
authors.lastname: Boisramé-Helms
mesh_terms: D000818:Animals; D004281:Docosahexaenoic Acids / Q000008:administration & dosage / Q000627:therapeutic use*; D015118:Eicosapentaenoic Acid / Q000008:administration & dosage / Q000627:therapeutic use*; D006439:Hemodynamics / Q000187:drug effects; D007275:Injections, Intravenous; D008297:Male; D009203:Myocardial Infarction / Q000097:blood* / Q000188:drug therapy*; D015428:Myocardial Reperfusion Injury / Q000097:blood* / Q000188:drug therapy*; D019001:Nitric Oxide Synthase / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D019210:Troponin I / Q000097:blood
chemical_list: D019210:Troponin I; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid; D019001:Nitric Oxide Synthase