title: Wound healing activity of the ethanol root extract and polyphenolic rich fraction from Potentilla fulgens.
abstract: BACKGROUND
  Potentilla fulgens Wall. ex Hook (Rosaceae) is a potent medicinal plant of the Western Himalayas, where its roots are traditionally used by the local people of Uttaranchal (India) to treat wounds and tiger bites.
  
  
  OBJECTIVE
  The present study scientifically evaluates the wound healing activity of P. fulgens ethanol root extract (EPF) and its ethyl acetate fraction (PFEA) on experimental rats.
  
  
  METHODS
  Wounds were inflicted on animals by using both excision and incision models. The wounded animals were treated for 16 days with EPF (oral: 200-400 mg/kg and topical: 5-10% w/w) and PFEA (oral: 75 mg/kg; topical: 1.75% w/w). Various physical (wound contraction, epithelialization rate, tensile strength) and biochemical parameters (hydroxyproline, hexosamine, proteins, DNA) were examined during the study. Oxidant product (lipidperoxidase), antioxidant enzymes (catalase, superoxide-dismutase) and reduced glutathione were determined. Morphological and histopathological studies of the skin tissues were monitored.
  
  
  RESULTS
  A significant (p < 0.05) wound healing property was observed when the animals were treated topically with EPF (10% w/w) and PFEA (1.75% w/w). A significantly (p < 0.05) increased in the levels of hydroxyproline, hexosamine, protein and DNA up to 59.22, 70.42, 61.01 and 60.00% was observed, respectively. This effect was further demonstrated by the morphological and histopathological representation, thus showing significant (p < 0.05) re-epethelialization on the healing area. EPF and PFEA also showed significant (p < 0.05) antioxidant activity.
  
  
  CONCLUSIONS
  The present study provided the scientific evidence, where P. fulgens rich in polyphenolic components possess remarkable wound healing activities, thereby supporting the traditional claims.
authors.affiliation: a Ayurvedic Pharmacy Research Laboratory , Rajiv Gandhi South Campus, Banaras Hindu University , Mirzapur , India.
authors.forename: Anindita
authors.initials: A
authors.lastname: Kundu
authors.affiliation: a Ayurvedic Pharmacy Research Laboratory , Rajiv Gandhi South Campus, Banaras Hindu University , Mirzapur , India.
authors.forename: Arka
authors.initials: A
authors.lastname: Ghosh
authors.affiliation: a Ayurvedic Pharmacy Research Laboratory , Rajiv Gandhi South Campus, Banaras Hindu University , Mirzapur , India.
authors.forename: Narendra K
authors.initials: NK
authors.lastname: Singh
authors.affiliation: a Ayurvedic Pharmacy Research Laboratory , Rajiv Gandhi South Campus, Banaras Hindu University , Mirzapur , India.
authors.forename: Gireesh K
authors.initials: GK
authors.lastname: Singh
authors.affiliation: a Ayurvedic Pharmacy Research Laboratory , Rajiv Gandhi South Campus, Banaras Hindu University , Mirzapur , India.
authors.forename: Ankit
authors.initials: A
authors.lastname: Seth
authors.affiliation: a Ayurvedic Pharmacy Research Laboratory , Rajiv Gandhi South Campus, Banaras Hindu University , Mirzapur , India.
authors.forename: Santosh K
authors.initials: SK
authors.lastname: Maurya
authors.affiliation: b Department of Pharmaceutics , Indian Institute of Technology (Banaras Hindu University) , Varanasi , India.
authors.forename: Siva
authors.initials: S
authors.lastname: Hemalatha
authors.affiliation: a Ayurvedic Pharmacy Research Laboratory , Rajiv Gandhi South Campus, Banaras Hindu University , Mirzapur , India.
authors.forename: Damiki
authors.initials: D
authors.lastname: Laloo
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D005260:Female; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology* / Q000633:toxicity; D018517:Plant Roots / Q000737:chemistry; D059808:Polyphenols / Q000494:pharmacology* / Q000633:toxicity; D031987:Potentilla / Q000737:chemistry*; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000187:drug effects / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D059808:Polyphenols
keywords: Antioxidant; HPTLC; collagen; excision wounds; re-pithelialization