title: PTEN at 18: Still Growing.
abstract: Discovered in 1997, PTEN remains one of the most studied tumor suppressors. In this issue of Methods in Molecular Biology, we assembled a series of papers describing various clinical and experimental approaches to studying PTEN function. Due to its broad expression, regulated subcellular localization, and intriguing phosphatase activity, methodologies aimed at PTEN study have often been developed in the context of mutations affecting various aspects of its regulation, found in patients burdened with PTEN loss-driven tumors. PTEN's extensive posttranslational modifications and dynamic localization pose unique challenges for studying PTEN features in isolation and necessitate considerable development of experimental systems to enable controlled characterization. Nevertheless, ongoing efforts towards the development of PTEN knockout and knock-in animals and cell lines, antibodies, and enzymatic assays have facilitated a huge body of work, which continues to unravel the fascinating biology of PTEN.
authors.affiliation: Princess Margaret Cancer Centre, University Health Network, Room 13-313, Toronto Medical Discovery Tower, 101 College Street, Toronto, M5G 1L7, ON, Canada.
authors.forename: Olena
authors.initials: O
authors.lastname: Gorbenko
authors.affiliation: University of Toronto, Princess Margaret Cancer Centre, Room 13-313, Toronto Medical Discovery Tower, 101 College Street, Toronto, M5G 1L7, ON, Canada. vuks@uhnres.utoronto.ca.
authors.forename: Vuk
authors.initials: V
authors.lastname: Stambolic
mesh_terms: D000818:Animals; D002460:Cell Line; D055879:Gene Knock-In Techniques; D055786:Gene Knockout Techniques; D006801:Humans; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000378:metabolism
chemical_list: D010716:Phosphatidylinositols; D010749:Phosphoprotein Phosphatases; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human
keywords: DNA damage repair; Genome integrity; PI3K signaling; PTEN; Phosphatase; Tumor suppressor