title: The association of the Klotho polymorphism rs9536314 with parameters of calcium-phosphate metabolism in patients on long-term hemodialysis.
abstract: BACKGROUND
  Patients on long-term hemodialysis frequently suffer from complications, such as secondary hyperparathyroidism, bone fractures, and arteriosclerosis. The process of regulating Ca/P metabolism depends on factors, such as FGF23 and Klotho. This study aimed to answer the question of whether the Klotho polymorphism rs9536314 is associated with FGF23 plasma concentration.
  
  
  METHODS
  In 118 patients undergoing hemodialysis, blood was collected before and after hemodialysis. The following parameters were measured in plasma: FGF23, serum: Ca, P, PTH, HGB, and iron concentrations. The KL gene polymorphism rs9536314 was identified by PCR-RFLP.
  
  
  RESULTS
  The KL polymorphism rs9536314 was not associated with Ca, P, PTH, or FGF23. There was a negative correlation between FGF23 and blood HGB levels and positive correlation between FGF23 and ESA dose.
  
  
  CONCLUSIONS
  The results obtained may indicate that there is no association between the KL polymorphism and FGF23 concentration in patients undergoing long-term.
authors.affiliation: a Department of Nephrology, Transplantology and Internal Diseases , Pomeranian Medical University , Szczecin , Poland ;
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Marchelek-Myśliwiec
authors.affiliation: a Department of Nephrology, Transplantology and Internal Diseases , Pomeranian Medical University , Szczecin , Poland ;
authors.forename: Jacek
authors.initials: J
authors.lastname: Różański
authors.affiliation: a Department of Nephrology, Transplantology and Internal Diseases , Pomeranian Medical University , Szczecin , Poland ;
authors.forename: Aldona
authors.initials: A
authors.lastname: Ogrodowczyk
authors.affiliation: a Department of Nephrology, Transplantology and Internal Diseases , Pomeranian Medical University , Szczecin , Poland ;
authors.forename: Grażyna
authors.initials: G
authors.lastname: Dutkiewicz
authors.affiliation: b Department of Medical Analytics , Pomeranian Medical University , Szczecin , Poland ;
authors.forename: Barbara
authors.initials: B
authors.lastname: Dołęgowska
authors.affiliation: b Department of Medical Analytics , Pomeranian Medical University , Szczecin , Poland ;
authors.forename: Daria
authors.initials: D
authors.lastname: Sałata
authors.affiliation: b Department of Medical Analytics , Pomeranian Medical University , Szczecin , Poland ;
authors.forename: Marta
authors.initials: M
authors.lastname: Budkowska
authors.affiliation: c Department of Biochemistry and Medical Chemistry , Pomeranian Medical University , Szczecin , Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Safranow
authors.affiliation: a Department of Nephrology, Transplantology and Internal Diseases , Pomeranian Medical University , Szczecin , Poland ;
authors.forename: Joanna
authors.initials: J
authors.lastname: Stępniewska
authors.affiliation: a Department of Nephrology, Transplantology and Internal Diseases , Pomeranian Medical University , Szczecin , Poland ;
authors.forename: Magda
authors.initials: M
authors.lastname: Wiśniewska
authors.affiliation: a Department of Nephrology, Transplantology and Internal Diseases , Pomeranian Medical University , Szczecin , Poland ;
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Ciechanowski
mesh_terms: D000328:Adult; D000368:Aged; D002118:Calcium / Q000378:metabolism*; D005260:Female; D000089703:Fibroblast Growth Factor-23; D005346:Fibroblast Growth Factors / Q000097:blood; D005966:Glucuronidase / Q000235:genetics*; D006801:Humans; D006962:Hyperparathyroidism, Secondary / Q000209:etiology / Q000378:metabolism; D007676:Kidney Failure, Chronic / Q000150:complications / Q000235:genetics / Q000378:metabolism / Q000628:therapy; D000090265:Klotho Proteins; D008297:Male; D008875:Middle Aged; D010710:Phosphates / Q000378:metabolism*; D006435:Renal Dialysis / Q000009:adverse effects / Q000379:methods; D013223:Statistics as Topic
chemical_list: C000717427:FGF23 protein, human; D010710:Phosphates; D005346:Fibroblast Growth Factors; D000089703:Fibroblast Growth Factor-23; D005966:Glucuronidase; D000090265:Klotho Proteins; D002118:Calcium
keywords: FGF23; Klotho polymorphism; hemodialysis