title: Cyclin D1 and cyclin E2 are differentially expressed in gastric cancer.
abstract: Cell cycle regulators cyclin D1 and cyclin E2 function in G1/S transition by activating downstream cyclin-dependent kinases. Deregulated expression of these cyclins has been reported in various cancers. However, little is known about their clinical significance in gastric carcinoma. We aimed to explore that whether there is differential expression of these cyclins in clinically distinct gastric cancer patients. In this study we recruited a total of 92 subjects including 20 controls and 72 cases of histopathologically proven gastric carcinoma. Expression profiling at transcript level was done by semiquantitative RT-PCR and of protein by immunohistochemistry. Receiver operator characteristics analysis was done for determining diagnostic utility of cyclin D1 and cyclin E2. We demonstrate that cyclins D1 and E2 are frequently overexpressed in early stages of gastric carcinoma. Interestingly, expression of cyclins D1 and E2 significantly correlates with different clinical parameters such as gender, histological type (intestinal and diffuse), tumor location (proximal, middle, and distal), tumor differentiation (differentiated and undifferentiated), tumor invasion (serosal, lymphatic, and venous) and tumor metastasis (lymph node, peritoneal, ascites, and liver). Cyclin D1 has significantly higher sensitivity and specificity as diagnostic biomarker than cyclin E2. Our results suggest that overexpression of cyclin D1 and cyclin E2 is an early event in gastric carcinogenesis. The differential expression of these cyclins may be useful as diagnostic biomarkers for early detection of gastric carcinoma.
authors.affiliation: Cancer Genetics Laboratory, Department of Molecular and Human Genetics, Banaras Hindu University, Varanasi, 221 005, India.
authors.forename: Soni
authors.initials: S
authors.lastname: Kumari
authors.affiliation: Department of General Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221 005, India.
authors.lastname: Puneet
authors.affiliation: Cancer Genetics Laboratory, Department of Molecular and Human Genetics, Banaras Hindu University, Varanasi, 221 005, India.|Department of Microbiology, Central University of Rajasthan, Kishangarh, 305 801, India.
authors.forename: Shyam Babu
authors.initials: SB
authors.lastname: Prasad
authors.affiliation: Cancer Genetics Laboratory, Department of Molecular and Human Genetics, Banaras Hindu University, Varanasi, 221 005, India.|Applied Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110 067, India.
authors.forename: Suresh Singh
authors.initials: SS
authors.lastname: Yadav
authors.affiliation: Department of Pathology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221 005, India.
authors.forename: Mohan
authors.initials: M
authors.lastname: Kumar
authors.affiliation: Department of General Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221 005, India.
authors.forename: A
authors.initials: A
authors.lastname: Khanna
authors.affiliation: Department of Gastroenterology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221 005, India.
authors.forename: V K
authors.initials: VK
authors.lastname: Dixit
authors.affiliation: Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221 005, India.
authors.forename: Gopal
authors.initials: G
authors.lastname: Nath
authors.affiliation: Department of Zoology, Mahila Mahavidyalaya, Banaras Hindu University, Varanasi, 221 005, India.
authors.forename: Sunita
authors.initials: S
authors.lastname: Singh
authors.affiliation: Cancer Genetics Laboratory, Department of Molecular and Human Genetics, Banaras Hindu University, Varanasi, 221 005, India. gnarayan@bhu.ac.in.
authors.forename: Gopeshwar
authors.initials: G
authors.lastname: Narayan
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism; D016022:Case-Control Studies; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism*; D016213:Cyclins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D012372:ROC Curve; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; C530104:CCND1 protein, human; C503688:CCNE2 protein, human; D016213:Cyclins; D019938:Cyclin D1
keywords: Clinical correlation; Cyclin D1; Cyclin E2; Gastric cancer