title: Crystal structure of the substrate-recognition domain of the Shigella E3 ligase IpaH9.8.
abstract: Infectious diseases caused by bacteria have significant impacts on global public health. During infection, pathogenic bacteria deliver a variety of virulence factors, called effectors, into host cells. The Shigella effector IpaH9.8 functions as an ubiquitin ligase, ubiquitinating the NF-κB essential modulator (NEMO)/IKK-γ to inhibit host inflammatory responses. IpaH9.8 contains leucine-rich repeats (LRRs) involved in substrate recognition and an E3 ligase domain. To elucidate the structural basis of the function of IpaH9.8, the crystal structure of the LRR domain of Shigella IpaH9.8 was determined and this structure was compared with the known structures of other IpaH family members. This model provides insights into the structural features involved in substrate specificity.
authors.affiliation: Graduate School of Life Science, University of Hyogo, 3-2-1 Kouto, Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Takagi
authors.affiliation: The Hakubi Center for Advanced Research, Kyoto University, Yoshida-konoe-cho, Sakyo-ku, Kyoto-shi, Kyoto 606-8501, Japan.
authors.forename: Minsoo
authors.initials: M
authors.lastname: Kim
authors.affiliation: Division of Bacterial Infection Biology, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
authors.forename: Chihiro
authors.initials: C
authors.lastname: Sasakawa
authors.affiliation: Graduate School of Life Science, University of Hyogo, 3-2-1 Kouto, Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan.
authors.forename: Tsunehiro
authors.initials: T
authors.lastname: Mizushima
mesh_terms: D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid; D012760:Shigella / Q000201:enzymology*; D013379:Substrate Specificity; D044767:Ubiquitin-Protein Ligases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D044767:Ubiquitin-Protein Ligases
keywords: E3 ligase; IpaH9.8; Shigella flexneri; effector; leucine-rich repeats