title: Roles of ERα during mouse trophectoderm lineage differentiation: revealed by antagonist and agonist of ERα.
abstract: During mouse early embryogenesis, blastomeres increase in number by the morula stage. Among them, the outer cells are polarized and differentiated into trophectoderm (TE), while the inner cells remain unpolarized and give rise to inner cell mass (ICM). TE provides an important liquid environment for ICM development. In spite of extensive research, the molecular mechanisms underlying TE formation are still obscure. In order to investigate the roles of estrogen receptor α (ERα) in this course, mouse 8-cell embryos were collected and cultured in media containing ERα specific antagonist MPP and/or agonist PPT. The results indicated that MPP treatment inhibits blastocyst formation in a dose-dependent manner, while PPT, at proper concentration, promotes the cavitation ratio of mouse embryos. Immunofluorescence staining results showed that MPP significantly decreased the nuclear expression of CDX2 in morula, but no significant changes of OCT4 were observed. Moreover, after MPP treatment, the expression levels of the genes related to TE specification, Tead4, Gata3 and Cdx2, were significantly reduced. Overall, these results indicated that ERα might affect mouse embryo cavitation by regulating TE lineage differentiation.
authors.affiliation: Cellular and Developmental Engineering Center, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350108, China.
authors.forename: Xiaoxiang
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350108, China.
authors.forename: Songhua
authors.initials: S
authors.lastname: Xu
authors.affiliation: Cellular and Developmental Engineering Center, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350108, China.
authors.forename: Chanchan
authors.initials: C
authors.lastname: Song
authors.affiliation: Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350108, China.
authors.forename: Lin
authors.initials: L
authors.lastname: He
authors.affiliation: Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350108, China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Lian
authors.affiliation: Cellular and Developmental Engineering Center, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350108, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Cellular and Developmental Engineering Center, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350108, China.|Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350108, China.
authors.forename: Jianen
authors.initials: J
authors.lastname: Wei
authors.affiliation: Cellular and Developmental Engineering Center, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350108, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Pang
authors.affiliation: Cellular and Developmental Engineering Center, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350108, China.|Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350108, China.
authors.forename: Shie
authors.initials: S
authors.lastname: Wang
mesh_terms: D015655:1-Methyl-4-phenylpyridinium / Q000494:pharmacology; D000818:Animals; D001483:Base Sequence; D053624:Blastocyst Inner Cell Mass / Q000166:cytology / Q000378:metabolism; D001757:Blastomeres / Q000166:cytology / Q000378:metabolism; D000071616:CDX2 Transcription Factor / Q000235:genetics; D002454:Cell Differentiation; D019070:Cell Lineage; D004268:DNA-Binding Proteins / Q000235:genetics; D004475:Ectoderm / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D047628:Estrogen Receptor alpha / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005260:Female; D050990:GATA3 Transcription Factor / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D008297:Male; D051379:Mice; D018613:Microscopy, Confocal; D009124:Muscle Proteins / Q000235:genetics; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D010636:Phenols / Q000494:pharmacology; D011720:Pyrazoles / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000091262:TEA Domain Transcription Factors; D014157:Transcription Factors / Q000235:genetics; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism*
chemical_list: D000071616:CDX2 Transcription Factor; C487514:Cdx2 protein, mouse; D004268:DNA-Binding Proteins; D047628:Estrogen Receptor alpha; D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D009124:Muscle Proteins; D050814:Octamer Transcription Factor-3; D010636:Phenols; C494174:Pou5f1 protein, mouse; D011720:Pyrazoles; D000091262:TEA Domain Transcription Factors; C101204:Tead4 protein, mouse; D014157:Transcription Factors; C486184:4,4',4''-(4-propyl-((1)H)-pyrazole-1,3,5-triyl) tris-phenol; D015655:1-Methyl-4-phenylpyridinium
keywords: blastocyst; differentiation; estrogen receptor alpha; morula; trophectoderm