title: A Simplified Method for the Aspiration of Bone Marrow from Patients Undergoing Hip and Knee Joint Replacement for Isolating Mesenchymal Stem Cells and In Vitro Chondrogenesis.
abstract: The procedure for aspiration of bone marrow from the femur of patients undergoing total knee arthroplasty (TKA) or total hip arthroplasty (THA) may vary from an OR (operating room) to OR based on the surgeon's skill and may lead to varied extent of clotting of the marrow and this, in turn, presents difficulty in the isolation of mesenchymal stem cells (MSCs) from such clotted bone marrow. We present a simple detailed protocol for aspirating bone marrow from such patients, isolation, and characterization of MSCs from the aspirated bone marrow specimens and show that the bone marrow presented no clotting or exhibited minimal clotting. This represents an economical source and convenient source of MSCs from bone marrow for use in regenerative medicine. Also, we presented the detailed protocol and showed that the MSCs derived from such bone marrow specimens exhibited MSCs characteristics and generated micromass cartilages, the recipe for regenerative medicine for osteoarthritis. The protocols we presented can be used as standard operating procedures (SOPs) by researchers and clinicians.
authors.affiliation: Arthritis Program, Orthopaedic Surgery, Toronto Western Hospital, 399 Bathurst Street, Toronto, ON, Canada M5T 2S8.
authors.forename: Subhash C
authors.initials: SC
authors.lastname: Juneja
authors.affiliation: Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON, Canada M5S 3G9; Krembil Research Institute, University Health Network, 399 Bathurst Street, Toronto, ON, Canada M5T 2S8.
authors.forename: Sowmya
authors.initials: S
authors.lastname: Viswanathan
authors.affiliation: STTARR Innovation Centre, Princess Margaret Cancer Centre, University Health Network, 101 College Street, Toronto, ON, Canada M5G 1L7.
authors.forename: Milan
authors.initials: M
authors.lastname: Ganguly
authors.affiliation: Arthritis Program, Orthopaedic Surgery, Toronto Western Hospital, 399 Bathurst Street, Toronto, ON, Canada M5T 2S8.
authors.forename: Christian
authors.initials: C
authors.lastname: Veillette