title: Increased expression of tissue inhibitor of metalloproteinase-1 correlates with improved outcome in canine cutaneous mast cell tumours.
abstract: Canine mast cell tumour (MCT) is a biologically heterogeneous disease. The extracellular matrix degradation promoted by matrix metalloproteinases (MMPs) has been studied in an attempt to elucidate the mechanisms involved in the biological behaviour of tumours. The aim of this study was to characterize the expression of MMP-2 and -9 and tissue inhibitors of metalloproteinase (TIMP)-1 and -2 in canine cutaneous MCTs and to evaluate their prognostic values. Immunohistochemical staining for MMP-2, MMP-9, TIMP-2 and TIMP-1 was performed in 46 canine cases of MCTs. TIMP-1 expression showed an independent prognostic value for post-surgical survival and disease-related mortality. Dogs with MCTs showing less than 22.9% mast cell TIMP-1 positivity were more prone to die because of the disease and had a shorter post-surgical survival. This article suggests the involvement of TIMP-1 in MCT progression, by contributing to a good outcome in patients with MCTs.
authors.affiliation: Departamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil.|Laboratório de Oncologia Comparada e Translacional, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, Brazil.
authors.forename: L H
authors.initials: LH
authors.lastname: Pulz
authors.affiliation: Departamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil.|Laboratório de Oncologia Comparada e Translacional, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, Brazil.
authors.forename: C N
authors.initials: CN
authors.lastname: Barra
authors.affiliation: Universidade Metodista de São Paulo, São Bernardo do Campo, Brazil.
authors.forename: S R
authors.initials: SR
authors.lastname: Kleeb
authors.affiliation: Universidade Paulista, São Paulo, Brazil.
authors.forename: J G
authors.initials: JG
authors.lastname: Xavier
authors.affiliation: Departamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: J L
authors.initials: JL
authors.lastname: Catão-Dias
authors.affiliation: Onco Cane Veterinária, São Paulo, Brazil.
authors.forename: R A
authors.initials: RA
authors.lastname: Sobral
authors.affiliation: Laboratório de Oncologia Comparada e Translacional, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, Brazil.
authors.forename: H
authors.initials: H
authors.lastname: Fukumasu
authors.affiliation: Laboratório de Oncologia Comparada e Translacional, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, Brazil.
authors.forename: R F
authors.initials: RF
authors.lastname: Strefezzi
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000175:diagnosis / Q000201:enzymology* / Q000401:mortality; D004285:Dogs; D005260:Female; D008297:Male; D054705:Mastocytoma, Skin / Q000175:diagnosis / Q000201:enzymology / Q000401:mortality / Q000662:veterinary*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D011379:Prognosis; D016019:Survival Analysis; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism*; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000378:metabolism
chemical_list: D019715:Tissue Inhibitor of Metalloproteinase-1; D019716:Tissue Inhibitor of Metalloproteinase-2; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9
keywords: TIMP-1; dog; extracellular matrix; immunohistochemistry; metalloproteinase; prognosis