title: Bone-Healing Capacity of PCL/PLGA/Duck Beak Scaffold in Critical Bone Defects in a Rabbit Model.
abstract: Bone defects are repaired using either natural or synthetic bone grafts. Poly(ϵ-caprolactone) (PCL), β-tricalcium phosphate (TCP), and poly(lactic-co-glycolic acid) (PLGA) are widely used as synthetic materials for tissue engineering. This study aimed to investigate the bone-healing capacity of PCL/PLGA/duck beak scaffold in critical bone defects and the oxidative stress status of the graft site in a rabbit model. The in vivo performance of 48 healthy New Zealand White rabbits, weighing between 2.5 and 3.5 kg, was evaluated. The rabbits were assigned to the following groups: group 1 (control), group 2 (PCL/PLGA hybrid scaffolds), group 3 (PCL/PLGA/TCP hybrid scaffolds), and group 4 (PCL/PLGA/DB hybrid scaffolds). A 5 mm critical defect was induced in the diaphysis of the left radius. X-ray, micro-CT, and histological analyses were conducted at (time 0) 4, 8, and 12 weeks after implantation. Furthermore, bone formation markers (bone-specific alkaline phosphatase, carboxyterminal propeptide of type I procollagen, and osteocalcin) were measured and oxidative stress status was determined. X-ray, micro-CT, biochemistry, and histological analyses revealed that the PCL/PLGA/duck beak scaffold promotes new bone formation in rabbit radius by inducing repair, suggesting that it could be a good option for the treatment of fracture.
authors.affiliation: College of Veterinary Medicine, Chungbuk National University, Cheongju 362-763, Republic of Korea.
authors.forename: Jae Yeon
authors.initials: JY
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine, Chungbuk National University, Cheongju 362-763, Republic of Korea.
authors.forename: Soo Jin
authors.initials: SJ
authors.lastname: Son
authors.affiliation: Korean Textile Development Institute, Daegu 703-712, Republic of Korea.
authors.forename: Jun Sik
authors.initials: JS
authors.lastname: Son
authors.affiliation: College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Seong Soo
authors.initials: SS
authors.lastname: Kang
authors.affiliation: College of Veterinary Medicine, Chungbuk National University, Cheongju 362-763, Republic of Korea.
authors.forename: Seok Hwa
authors.initials: SH
authors.lastname: Choi
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000627:therapeutic use*; D001861:Bone Regeneration; D016025:Bone Transplantation / Q000379:methods; D002130:Calcium Phosphates / Q000737:chemistry / Q000627:therapeutic use; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry / Q000627:therapeutic use; D010012:Osteogenesis / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D011091:Polyesters / Q000737:chemistry / Q000627:therapeutic use; D011100:Polyglycolic Acid / Q000737:chemistry / Q000627:therapeutic use; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D011091:Polyesters; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; C016240:polycaprolactone; D011100:Polyglycolic Acid; D019344:Lactic Acid; C018392:tricalcium phosphate