title: Impact of β-glucan on the Fecal Water Genotoxicity of Polypectomized Patients.
abstract: The aim of the study was to determine the effect of β-glucan on the cytotoxicity and genotoxicity of polypectomized patient's fecal water (FW). Polypectomized volunteers (n = 69) were randomly assigned to consume bread with or without β-glucan, for 3 months. FW was collected at the beginning (t = 0), the 30th and 90th day and 2 wk after the intervention. Cytotoxicity and genotoxicity were estimated on Caco-2 cells, using trypan blue exclusion test and comet assay, respectively. Gastrointestinal symptoms were recorded and subjects kept a 3-day food diary at baseline and after completion. Trypan blue exclusion test revealed cell survival of approximately 87% after incubation with FW. The FW samples showed 49% genotoxicity at the baseline. Genotoxicity in the intervention group decreased during the trial reaching statistical significance on the 90th day compared to control. An increase was noticed 2 wk after the trial, but it still remained significantly lower compared to control. Group-specific analysis for β-glucan also revealed significant decrease in the genotoxicity on the 90th day compared to baseline. β-glucan ingestion in polypectomized patients significantly decreased the genotoxicity of their FW. Our findings suggest that β-glucan consumption could possibly provide protection against colon cancer development.
authors.affiliation: a Laboratory of Biology, Biochemistry, Physiology and Microbiology, Harokopio University , 70, El Venizelou str., Kallithea , Greece.
authors.forename: Katja T
authors.initials: KT
authors.lastname: Turunen
authors.affiliation: b Institute of Biology, Medicinal Chemistry and Biotechnology, National Hellenic Research Foundation , Athens , Greece.
authors.forename: Vasiliki
authors.initials: V
authors.lastname: Pletsa
authors.affiliation: b Institute of Biology, Medicinal Chemistry and Biotechnology, National Hellenic Research Foundation , Athens , Greece.
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Georgiadis
authors.affiliation: c IBD Unit, IASO General Hospital , Athens , Greece.
authors.forename: John K
authors.initials: JK
authors.lastname: Triantafillidis
authors.affiliation: d Second Department of Gastroenterology , Evangelismos Hospital , Athens , Greece.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Karamanolis
authors.affiliation: a Laboratory of Biology, Biochemistry, Physiology and Microbiology, Harokopio University , 70, El Venizelou str., Kallithea , Greece.
authors.forename: Adamantini
authors.initials: A
authors.lastname: Kyriacou
mesh_terms: D000368:Aged; D003111:Colonic Polyps; D020552:Comet Assay; D005243:Feces / Q000737:chemistry*; D005260:Female; D001467:Hordeum / Q000737:chemistry; D006801:Humans; D008297:Male; D008875:Middle Aged; D009152:Mutagenicity Tests; D014867:Water; D047071:beta-Glucans / Q000494:pharmacology*
chemical_list: D047071:beta-Glucans; D014867:Water