title: BSA-binding properties and anti-proliferative effects of amino acids functionalized polyoxomolybdates.
abstract: Glycine decorated heteropolymolybdates, K2Na[AsMo6O21(O2CCH2NH3)3]·6H2O 1 and K2Na2[γ-Mo8O26(O2CCH2NH3)2]·6H2O 2, have been synthesized and evaluated for in vitro anti-proliferative effects. The identity and high purity of compounds 1 and 2 were confirmed by elemental analysis, FT-IR spectrum, UV-vis spectrum, and X-ray diffraction. Crystal data for 2: triclinic, P-1, a=9.792(2)Å, b=10.077(2)Å, c=10.351(2)Å, α=83.865(4)°, β=71.110(4)°, γ=62.284(3)°, V=854.3(3)Å(3), Z=1, R(final)=0.0486. The inhibitory effects of 1 and 2 on human non-small cell lung cancer cell line A549 were investigated by MTT assay, nuclear staining, and the flow cytometry. It indicated that compound 1 inhibited the proliferation of A549 cells in a dose-dependent and time-dependent manner, which is more effective than the positive control, 5-fluorouracil (5-FU) (P<0.05). The staining and flow cytometry results showed that compound 1 induced the apoptosis and necrosis of A549 cells and inhibit cell proliferation, which is associated with S-phase arrest. Compound 2 showed a modest activity in a dose-dependent manner. In addition, the interaction between compound 1 and bovine serum albumin (BSA) was evaluated by spectroscopic methods. The results showed that the compound 1 effectively quenched the intrinsic fluorescence of BSA via static quenching and changed the conformation of BSA.
authors.affiliation: School of Public Health, Jilin University, Changchun, Jilin 130021, PR China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Li
authors.affiliation: School of Public Health, Jilin University, Changchun, Jilin 130021, PR China.
authors.forename: Wen
authors.initials: W
authors.lastname: Qi
authors.affiliation: School of Public Health, Jilin University, Changchun, Jilin 130021, PR China.
authors.forename: Hongqian
authors.initials: H
authors.lastname: Cao
authors.affiliation: School of Public Health, Jilin University, Changchun, Jilin 130021, PR China. Electronic address: qiyanfei@jlu.edu.cn.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Qi
authors.affiliation: State Key Laboratory Integrated Optoelectronics, College of Electronic Science and Engineering, Jinlin University, Changchun 130012, China. Electronic address: zhangs@jlu.edu.cn.
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory Integrated Optoelectronics, College of Electronic Science and Engineering, Jinlin University, Changchun 130012, China.
authors.forename: Shihan
authors.initials: S
authors.lastname: Xu
authors.affiliation: School of Public Health, Jilin University, Changchun, Jilin 130021, PR China.
authors.forename: Jiaheng
authors.initials: J
authors.lastname: Sun
authors.affiliation: School of Public Health, Jilin University, Changchun, Jilin 130021, PR China.
authors.forename: Shuanli
authors.initials: S
authors.lastname: Guo
mesh_terms: D000072283:A549 Cells; D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002453:Cell Cycle / Q000187:drug effects; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D005434:Flow Cytometry; D006801:Humans; D008982:Molybdenum / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D000596:Amino Acids; D012710:Serum Albumin, Bovine; D008982:Molybdenum
keywords: Anti-proliferative effect; Bovine serum albumin; In vitro; Polyoxometalates