title: Effects of Silk Sericin on Incision Wound Healing in a Dorsal Skin Flap Wound Healing Rat Model.
abstract: BACKGROUND
  The wound healing process is complex and still poorly understood. Sericin is a silk protein synthesized by silk worms (Bombyx mori). The objective of this study was to evaluate in vivo wound healing effects of a sericin-containing gel formulation in an incision wound model in rats.
  
  
  METHODS
  Twenty-eight Wistar-Albino rats were divided into 4 groups (n=7). No intervention or treatment was applied to the Intact control group. For other groups, a dorsal skin flap (9×3 cm) was drawn and pulled up with sharp dissection. The Sham operated group received no treatment. The Placebo group received placebo gel without sericin applied to the incision area once a day from day 0 to day 9. The Sericin Group 3 received 1% sericin gel applied to the incision area once a day from day 0 to day 9. Hematoxylin and eosin stain was applied for histological analysis and Mallory-Azan staining was applied for histoimmunochemical analysis of antibodies and iNOS (inducible nitric oxide synthase), and desmin was applied to paraffin sections of skin wound specimens. Parameters of oxidative stress were measured in the wound area.
  
  
  RESULTS
  Epidermal thickness and vascularization were increased, and hair root degeneration, edema, cellular infiltration, collagen discoloration, and necrosis were decreased in Sericin group in comparison to the Placebo group and the Sham operated group. Malonyldialdehyde (MDA) levels were decreased, but superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities were increased in the sericin group.
  
  
  CONCLUSIONS
  We found that sericin had significant positive effects on wound healing and antioxidant activity. Sericin-based formulations can improve healing of incision wounds.
authors.affiliation: Department of Emergency Medicine, Ege University, Faculty of Medicine, Bornova, Izmir, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Ersel
authors.affiliation: Department of Histology and Embryology, Ege University, Faculty of Medicine, Bornova, Izmir, Turkey.
authors.forename: Yigit
authors.initials: Y
authors.lastname: Uyanikgil
authors.affiliation: Department of Emergency Medicine, Ege University, Faculty of Medicine, Bornova, Izmir, Turkey.
authors.forename: Funda
authors.initials: F
authors.lastname: Karbek Akarca
authors.affiliation: Department of Emergency Medicine, Ege University, Faculty of Medicine, Bornova, Izmir, Turkey.
authors.forename: Enver
authors.initials: E
authors.lastname: Ozcete
authors.affiliation: Department of Emergency Medicine, Ege University, Faculty of Medicine, Bornova, Izmir, Turkey.
authors.forename: Yusuf Ali
authors.initials: YA
authors.lastname: Altunci
authors.affiliation: Department of Biotechnology, Graduate School of Natural and Applied Sciences, Ege University, Bornova, Izmir, Turkey.
authors.forename: Fatih
authors.initials: F
authors.lastname: Karabey
authors.affiliation: Department of Histology and Embryology, Ege University, Faculty of Medicine, Bornova, Izmir, Turkey.
authors.forename: Turker
authors.initials: T
authors.lastname: Cavusoglu
authors.affiliation: Department of Biochemistry, Evliya Celebi Training and Research Hospital, Faculty of Medicine, Dumlupınar University, Kutahya, Turkey.
authors.forename: Ayfer
authors.initials: A
authors.lastname: Meral
authors.affiliation: Department of Histology and Embryology, Ege University, Faculty of Medicine, Bornova, Izmir, Turkey.
authors.forename: Gurkan
authors.initials: G
authors.lastname: Yigitturk
authors.affiliation: Department of Biopharmaceutics and Pharmacokinetics, Ege University, Faculty of Pharmacy, Bornova, Izmir, Turkey.
authors.forename: Emel
authors.initials: E
authors.lastname: Oyku Cetin
mesh_terms: D000818:Animals; D001706:Biopsy; D002374:Catalase / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000378:metabolism; D004195:Disease Models, Animal; D004487:Edema / Q000473:pathology; D004817:Epidermis / Q000187:drug effects / Q000473:pathology; D005979:Glutathione Peroxidase / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009336:Necrosis; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010919:Placebos; D017208:Rats, Wistar; D047030:Sericins / Q000737:chemistry / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects / Q000473:pathology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013524:Surgical Flaps / Q000473:pathology*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D010919:Placebos; D047030:Sericins; D008315:Malondialdehyde; D003094:Collagen; D002374:Catalase; D005979:Glutathione Peroxidase; D052247:Nitric Oxide Synthase Type II; D013482:Superoxide Dismutase