title: miR-590 promotes cell proliferation and invasion in T-cell acute lymphoblastic leukaemia by inhibiting RB1.
abstract: MicroRNAs play important roles in the pathogenesis of cancers by inhibiting gene expression at posttranscriptional level. Here, we identified that miR-590 and its predicted target gene RB1 are differentially expressed in T-cell acute lymphoblastic leukaemia (T-ALL). The correlation between miR-590 and RB1 was further confirmed in 395 T-ALL patients. In T-ALL cell lines, miR-590 promoted the cell proliferation by increasing G1/S transition. Moreover, migration and invasion assay showed that miR-590 promotes the migration and invasion of T-ALL cells by increasing E-cadherin and inhibiting MMP-9. Luciferase assays confirmed that miR-590 directly binds to the 3'untranslated region of RB1, and western blotting showed that miR-590 suppresses the expression of RB1 at the protein levels. This study indicated that miR-590 inhibits RB1 and promotes proliferation and invasion of T-ALL cells. Thus, miR-590 may represent a potential therapeutic target for T-ALL intervention.
authors.affiliation: Department of Clinical Laboratory Diagnosis, Children's Hospital of Soochow University, Suzhou, China.
authors.forename: Mei-Hua
authors.initials: MH
authors.lastname: Miao
authors.affiliation: Department of Clinical Laboratory Diagnosis, Children's Hospital of Soochow University, Suzhou, China.
authors.forename: Xue-Qiang
authors.initials: XQ
authors.lastname: Ji
authors.affiliation: Department of Hematology, Affiliated Hospital of Jining Medical University, Jining, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory Diagnosis, Children's Hospital of Soochow University, Suzhou, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Clinical Laboratory Diagnosis, Children's Hospital of Soochow University, Suzhou, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Clinical Laboratory Diagnosis, Children's Hospital of Soochow University, Suzhou, China.
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Shao
mesh_terms: D020413:3' Untranslated Regions; D000293:Adolescent; D000328:Adult; D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism; D002453:Cell Cycle; D002465:Cell Movement; D049109:Cell Proliferation; D002648:Child; D002675:Child, Preschool; D005260:Female; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D016130:Immunophenotyping; D007223:Infant; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D035683:MicroRNAs / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000276:immunology; D056286:Retinoblastoma Binding Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D055815:Young Adult
chemical_list: D020413:3' Untranslated Regions; D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; C568221:MIRN590 microRNA, human; D035683:MicroRNAs; C000606393:RB1 protein, human; D056286:Retinoblastoma Binding Proteins; D044767:Ubiquitin-Protein Ligases; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: RB1; T-ALL; cancer; miR-590