title: Submerged macrophytes shape the abundance and diversity of bacterial denitrifiers in bacterioplankton and epiphyton in the Shallow Fresh Lake Taihu, China.
abstract: nirK and nirS genes are important functional genes involved in the denitrification pathway. Recent studies about these two denitrifying genes are focusing on sediment and wastewater microbe. In this study, we conducted a comparative analysis of the abundance and diversity of denitrifiers in the epiphyton of submerged macrophytes Potamogeton malaianus and Ceratophyllum demersum as well as in bacterioplankton in the shallow fresh lake Taihu, China. Results showed that nirK and nirS genes had significant different niches in epiphyton and bacterioplankton. Bacterioplankton showed greater abundance of nirK gene in terms of copy numbers and lower abundance of nirS gene. Significant difference in the abundance of nirK and nirS genes also existed between the epiphyton from different submerged macrophytes. Similar community diversity yet different community abundance was observed between epiphytic bacteria and bacterioplankton. No apparent seasonal variation was found either in epiphytic bacteria or bacterioplankton; however, environmental parameters seemed to have direct relevancy with nirK and nirS genes. Our study suggested that submerged macrophytes have greater influence than seasonal parameters in shaping the presence and abundance of bacterial denitrifiers. Further investigation needs to focus on the potential contact and relative contribution between denitrifiers and environmental factors.
authors.affiliation: Jiangsu Key Laboratory of Environmental Change and Ecological Construction, Nanjing Normal University, Nanjing, 210023, China.|Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, School of Geography Science, Nanjing Normal University, Nanjing, 210023, China.|Jiangsu Provincial Key Laboratory of Materials Cycling and Pollution Control, Nanjing Normal University, Nanjing, 210023, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Fan
authors.affiliation: Jiangsu Key Laboratory of Environmental Change and Ecological Construction, Nanjing Normal University, Nanjing, 210023, China.|Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, School of Geography Science, Nanjing Normal University, Nanjing, 210023, China.|Jiangsu Provincial Key Laboratory of Materials Cycling and Pollution Control, Nanjing Normal University, Nanjing, 210023, China.
authors.forename: Rui-Ming
authors.initials: RM
authors.lastname: Han
authors.affiliation: Jiangsu Key Laboratory of Environmental Change and Ecological Construction, Nanjing Normal University, Nanjing, 210023, China.|Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, School of Geography Science, Nanjing Normal University, Nanjing, 210023, China.|Jiangsu Provincial Key Laboratory of Materials Cycling and Pollution Control, Nanjing Normal University, Nanjing, 210023, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
authors.affiliation: Jiangsu Key Laboratory of Environmental Change and Ecological Construction, Nanjing Normal University, Nanjing, 210023, China. wangguoxiang@njnu.edu.cn.|Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, School of Geography Science, Nanjing Normal University, Nanjing, 210023, China. wangguoxiang@njnu.edu.cn.|Jiangsu Provincial Key Laboratory of Materials Cycling and Pollution Control, Nanjing Normal University, Nanjing, 210023, China. wangguoxiang@njnu.edu.cn.|Nanjing Normal University, School of Geography Science, Wenyuan Road 1, Nanjing, 210023, China. wangguoxiang@njnu.edu.cn.
authors.forename: Guo-Xiang
authors.initials: GX
authors.lastname: Wang
mesh_terms: D059001:Aquatic Organisms / Q000378:metabolism; D001419:Bacteria / Q000201:enzymology / Q000235:genetics*; D044822:Biodiversity; D002681:China; D058440:Denitrification; D060106:Lakes / Q000382:microbiology; D019684:Magnoliopsida; D059013:Microbial Consortia; D009572:Nitrite Reductases / Q000235:genetics; D010933:Plankton / Q000201:enzymology / Q000235:genetics*; D031821:Potamogetonaceae / Q000382:microbiology*; D012621:Seasons
chemical_list: D009572:Nitrite Reductases
keywords: Bacterioplankton; Denitrifier; Epiphytic bacteria; Submerged macrophytes; Taihu Lake