title: Nonspecific protein labeling of photoaffinity linkers correlates with their molecular shapes in living cells.
abstract: Herein we report molecular shape-dependent nonspecific labeling of photoaffinity linkers (PLs) in the cellular proteome. Linear PLs have a greater tendency to engage in nonspecific binding than branched PLs. Exploiting this property, we discovered a smaller branched diazirine-based PL as the best photoaffinity probe with minimal nonspecific binding characteristics from among 5 probes with different PLs.
authors.affiliation: Department of Biophysics and Chemical Biology, Seoul National University, Seoul, 151-747, Korea. sbpark@snu.ac.kr pls6443@snu.ac.kr.
authors.forename: Hankum
authors.initials: H
authors.lastname: Park
authors.forename: Ja Young
authors.initials: JY
authors.lastname: Koo
authors.forename: Yellamelli V V
authors.initials: YV
authors.lastname: Srikanth
authors.forename: Sangmi
authors.initials: S
authors.lastname: Oh
authors.forename: Jiyoon
authors.initials: J
authors.lastname: Lee
authors.forename: Jongmin
authors.initials: J
authors.lastname: Park
authors.forename: Seung Bum
authors.initials: SB
authors.lastname: Park
mesh_terms: D001577:Benzophenones / Q000737:chemistry*; D057930:Click Chemistry; D003978:Diazomethane / Q000031:analogs & derivatives* / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D006367:HeLa Cells; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D015394:Molecular Structure; D019920:Photoaffinity Labels / Q000737:chemistry*; D014404:Tubulin / Q000032:analysis*
chemical_list: D001577:Benzophenones; D019920:Photoaffinity Labels; D014404:Tubulin; D003978:Diazomethane; D007770:L-Lactate Dehydrogenase