title: Host genetic influence on papillomavirus-induced tumors in the horse.
abstract: The common equine skin tumors known as sarcoids have been causally associated with infection by bovine papillomavirus (BPV). Additionally, there is evidence for host genetic susceptibility to sarcoids. We investigated the genetic basis of susceptibility to sarcoid tumors on a cohort of 82 affected horses and 270 controls genotyped on a genome-wide platform and two custom panels. A Genome Wide Association Study (GWAS) identified candidate regions on six chromosomes. Bayesian probability analysis of the same dataset verified only the regions on equine chromosomes (ECA) 20 and 22. Fine mapping using custom-produced SNP arrays for ECA20 and ECA22 regions identified two marker loci with high levels of significance: SNP BIEC2-530826 (map position 32,787,619) on ECA20 in an intron of the DQA1 gene in the Major Histocompatibility Complex (MHC) class II region (p = 4.6e-06), and SNP BIEC2-589604 (map position 25,951,536) on ECA22 in a 200 kb region containing four candidate genes: PROCR, EDEM2, EIF6 and MMP24 (p = 2.14e-06). The marker loci yielded odds ratios of 5.05 and 4.02 for ECA20 and ECA22, respectively. Associations between genetic MHC class II variants and papillomavirus-induced tumors have been reported for human papillomavirus and cottontail rabbit papillomavirus infections. This suggests a common mechanism for susceptibility to tumor progression that may involve subversion of the host immune response. This study also identified a genomic region other than MHC that influenced papillomavirus-induced tumor development in the studied population.
authors.affiliation: Department of Animal Science, Cornell University, Ithaca, NY.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Staiger
authors.affiliation: Baker Institute for Animal Health, Cornell University, Ithaca, NY.
authors.forename: Chia T
authors.initials: CT
authors.lastname: Tseng
authors.affiliation: Baker Institute for Animal Health, Cornell University, Ithaca, NY.
authors.forename: Donald
authors.initials: D
authors.lastname: Miller
authors.affiliation: Baker Institute for Animal Health, Cornell University, Ithaca, NY.
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Cassano
authors.affiliation: MRC-University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
authors.forename: Lubna
authors.initials: L
authors.lastname: Nasir
authors.affiliation: Department of Animal Science, Iowa State University, Ames, IA.
authors.forename: Dorian
authors.initials: D
authors.lastname: Garrick
authors.affiliation: Department of Animal Science, University of Florida, Gainesville, FL.
authors.forename: Samantha A
authors.initials: SA
authors.lastname: Brooks
authors.affiliation: Baker Institute for Animal Health, Cornell University, Ithaca, NY.
authors.forename: Douglas F
authors.initials: DF
authors.lastname: Antczak
mesh_terms: D000483:Alleles; D000818:Animals; D016022:Case-Control Studies; D002874:Chromosome Mapping; D020022:Genetic Predisposition to Disease; D055106:Genome-Wide Association Study; D006734:Horse Diseases / Q000453:epidemiology* / Q000209:etiology*; D006736:Horses; D015810:Linkage Disequilibrium; D009369:Neoplasms / Q000662:veterinary*; D016017:Odds Ratio; D030361:Papillomavirus Infections / Q000150:complications* / Q000821:virology; D020641:Polymorphism, Single Nucleotide; D012878:Skin Neoplasms / Q000453:epidemiology / Q000209:etiology
keywords: GWAS; MHC; horse; papillomavirus; sarcoid tumor