title: [Overexpression of PPARγ induces adipogenic steatosis in mouse primary hepatocytes].
abstract: To investigate the effects of PPARγ overexpression on steatosis in mouse primary hepatocytes.
  
  
  
  Primary hepatocytes isolated from C57BL/6J mice were infected with either Ad/LacZ or Ad/PPARγ for 48 h. Steatosis of the primary hepatocytes was checked by Oil Red O staining. The mRNA and protein expression of adipocyte-specific genes PPARγ, aP2 and CideA were analyzed by using RT Real-time PCR and Western Blot.
  
  
  
  Primary hepatocytes were small and even. Hepatocyte nuclei were round with dispersed chromatin and prominent nucleoli. Accumulated lipid droplets were observed in Ad/PPARγ-infected hepatocytes, but in Ad/LacZ-infected hepatocytes. Moreover, compared with Ad/LacZ-infected hepatocytes, the mRNA expression of PPARγ, aP2, FGF21 and CideA in Ad/PPARγ-infected hepatocytes were significantly induced, the protein expression of PPARγ and its target aP2 strongly increased.
  
  
  
  over expression of PPARγ induces adipogenic steatosis in mouse primary hepatocytes.
authors.affiliation: Research Institute of Atherosclerotic Disease, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China;Laboratory Animal Center, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Bai
authors.affiliation: Research Institute of Atherosclerotic Disease, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China;Laboratory Animal Center, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Research Institute of Atherosclerotic Disease, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China;Laboratory Animal Center, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Xie
authors.affiliation: Research Institute of Atherosclerotic Disease, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China;Laboratory Animal Center, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.affiliation: Research Institute of Atherosclerotic Disease, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China;Laboratory Animal Center, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China.
authors.forename: Sihai
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Pathology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.
authors.forename: Yuzhi
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Research Institute of Atherosclerotic Disease, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China;Laboratory Animal Center, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China.
authors.forename: Enqi
authors.initials: E
authors.lastname: Liu
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D050156:Adipogenesis; D000818:Animals; D002478:Cells, Cultured; D005234:Fatty Liver / Q000378:metabolism / Q000473:pathology*; D005822:Genetic Vectors; D022781:Hepatocytes / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D047495:PPAR gamma / Q000378:metabolism*; D014162:Transfection
chemical_list: D047495:PPAR gamma