title: Rapid characterisation of Klebsiella oxytoca isolates from contaminated liquid hand soap using mass spectrometry, FTIR and Raman spectroscopy.
abstract: Microbiological monitoring of consumer products and the efficiency of early warning systems and outbreak investigations depend on the rapid identification and strain characterisation of pathogens posing risks to the health and safety of consumers. This study evaluates the potential of three rapid analytical techniques for identification and subtyping of bacterial isolates obtained from a liquid hand soap product, which has been recalled and reported through the EU RAPEX system due to its severe bacterial contamination. Ten isolates recovered from two bottles of the product were identified as Klebsiella oxytoca and subtyped using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI TOF MS), near-infrared Fourier transform (NIR FT) Raman spectroscopy and Fourier transform infrared (FTIR) spectroscopy. Comparison of the classification results obtained by these phenotype-based techniques with outcomes of the DNA-based methods pulsed-field gel electrophoresis (PFGE), multi-locus sequence typing (MLST) and single nucleotide polymorphism (SNP) analysis of whole-genome sequencing (WGS) data revealed a high level of concordance. In conclusion, a set of analytical techniques might be useful for rapid, reliable and cost-effective microbial typing to ensure safe consumer products and allow source tracking.
authors.affiliation: Federal Institute for Risk Assessment, Department of Biological Safety, Max-Dohrn-Str. 8-10, D-10589 Berlin, Germany. ralf.dieckmann@bfr.bund.de.
authors.forename: Ralf
authors.initials: R
authors.lastname: Dieckmann
authors.affiliation: Federal Institute for Risk Assessment, Department of Biological Safety, Max-Dohrn-Str. 8-10, D-10589 Berlin, Germany. ralf.dieckmann@bfr.bund.de.
authors.forename: Jens Andre
authors.initials: JA
authors.lastname: Hammerl
authors.affiliation: Landesamt für Verbraucherschutz Sachsen-Anhalt, Fachbereich Lebensmittelsicherheit, Freiimfelder Str. 68, D-06112 Halle, Germany.
authors.forename: Hartmut
authors.initials: H
authors.lastname: Hahmann
authors.affiliation: Landesamt für Verbraucherschutz Sachsen-Anhalt, Fachbereich Lebensmittelsicherheit, Freiimfelder Str. 68, D-06112 Halle, Germany.
authors.forename: Amal
authors.initials: A
authors.lastname: Wicke
authors.affiliation: Federal Institute for Risk Assessment, Department of Biological Safety, Max-Dohrn-Str. 8-10, D-10589 Berlin, Germany. ralf.dieckmann@bfr.bund.de.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Kleta
authors.affiliation: Robert Koch-Institut, NG4 - Bioinformatics, Seestraße 10, D-13353 Berlin, Germany.
authors.forename: Piotr Wojciech
authors.initials: PW
authors.lastname: Dabrowski
authors.affiliation: Robert Koch-Institut, ZBS 1 - Highly Pathogenic Viruses, Seestraße 10, D-13353 Berlin, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Nitsche
authors.affiliation: Robert Koch-Institut, ZBS 6 - Proteomics and Spectroscopy, Seestraße 10, D-13353 Berlin, Germany.
authors.forename: Maren
authors.initials: M
authors.lastname: Stämmler
authors.affiliation: Federal Institute for Risk Assessment, Department of Biological Safety, Max-Dohrn-Str. 8-10, D-10589 Berlin, Germany. ralf.dieckmann@bfr.bund.de.
authors.forename: Sascha
authors.initials: S
authors.lastname: Al Dahouk
authors.affiliation: Robert Koch-Institut, ZBS 6 - Proteomics and Spectroscopy, Seestraße 10, D-13353 Berlin, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Lasch
mesh_terms: D004340:Drug Contamination; D006801:Humans; D041121:Klebsiella oxytoca / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D058885:Multilocus Sequence Typing; D012915:Soaps / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D013997:Time Factors
chemical_list: D012915:Soaps