title: MMP13, TIMP2 and TGFB3 Gene Polymorphisms in Brazilian Chronic Periodontitis and Periimplantitis Subjects.
abstract: Subjects susceptible to chronic periodontitis (CP) show a high risk for the development of periimplantitis (PI). Both diseases are multifactorial, presenting similarities in their pathophysiology and polygenic profile. MMP-13 (matrix metalloproteinases 13/ collagenase 3) is a collagenolytic enzyme, which expression is induced by TGF beta 3 (transforming growth factor type 3) in human gingival fibroblasts and inhibited by TIMP-2 (tissue inhibitor of metalloproteinase type 2). The aim of this study was to investigate the occurrence of periimplantitis (PI) in subjects with history of chronic periodontitis (CP) and polymorphisms frequency in MMP13, TIMP2 and TGFB3 genes. One hundred and sixty-three volunteers received dental implant placement were submitted to oral and radiographic examination in order to identify past history of CP or presence of PI. Volunteers were divided into 4 groups: Control (without PI and CP, n=72), CP (with CP and without PI, n=28), PI (with PI and without CP, n=28) and diseased (with CP and PI, n=35). The chi-square test correlated genotypes in specific regions of MMP13 (rs2252070), TIMP2 (rs7501477) and TGFB3 (rs2268626) genes, considering the interaction between CP and PI. The results showed that volunteers with CP had 3.2 times more susceptibility to develop PI (p=0.0004) compared to those without CP. No significant association was observed in MMP13, TIMP2 and TGFB3 genes with CP or PI. CP is a risk factor to develop PI, however, there is no association of both diseases with polymorphisms in the MMP13, TIMP2 and TGFB3 genes.
authors.affiliation: Postgraduate Program in Dentistry, Universidade Federal Fluminense, Niterói, RJ, Brazil.
authors.forename: Roberto
authors.initials: R
authors.lastname: Gonçalves Junior
authors.affiliation: Dental Clinical Department, Universidade Federal Fluminense, Niterói, RJ, Brazil.
authors.forename: Aristides da Rosa
authors.initials: Ada R
authors.lastname: Pinheiro
authors.affiliation: Oral Implantology Post-graduation, Universidade Federal Fluminense, Niterói, RJ, Brazil.
authors.forename: José Jorge
authors.initials: JJ
authors.lastname: Schoichet
authors.affiliation: Oral Implantology Post-graduation, Universidade Federal Fluminense, Niterói, RJ, Brazil.
authors.forename: Carlos Henrique Ramirez
authors.initials: CH
authors.lastname: Nunes
authors.affiliation: Oral Implantology Post-graduation, Universidade Federal Fluminense, Niterói, RJ, Brazil.
authors.forename: Rackel
authors.initials: R
authors.lastname: Gonçalves
authors.affiliation: Postgraduate Program in Dentistry, Universidade Federal Fluminense, Niterói, RJ, Brazil.
authors.forename: Leticia Ladeira
authors.initials: LL
authors.lastname: Bonato
authors.affiliation: Postgraduate Program in Dentistry, Universidade Federal Fluminense, Niterói, RJ, Brazil.
authors.forename: Valquiria
authors.initials: V
authors.lastname: Quinelato
authors.affiliation: Departament of Specific Formation, School of Dentistry, Universidade Federal Fluminense, Nova Friburgo, RJ, Brazil.
authors.forename: Leonardo Santos
authors.initials: LS
authors.lastname: Antunes
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Erika Calvano
authors.initials: EC
authors.lastname: Küchler
authors.affiliation: Clinical Research Unit and Biology Institute, Universidade Federal Fluminense, Niterói, RJ, Brazil.
authors.forename: Julie
authors.initials: J
authors.lastname: Lobo
authors.affiliation: Postgraduate Program in Dentistry, Universidade Federal Fluminense, Niterói, RJ, Brazil.
authors.forename: Ricardo de Mello
authors.initials: Rde M
authors.lastname: Villas-Bôas
authors.affiliation: Department of Oral Biology, School of Dental Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
authors.forename: Alexandre Rezende
authors.initials: AR
authors.lastname: Vieira
authors.affiliation: Quality and Technology, National Institute of Metrology, Rio de Janeiro, RJ, Brazil.
authors.forename: José Mauro
authors.initials: JM
authors.lastname: Granjeiro
authors.affiliation: Postgraduate Program in Dentistry, Universidade Federal Fluminense, Niterói, RJ, Brazil.
authors.forename: Priscila Ladeira
authors.initials: PL
authors.lastname: Casado
mesh_terms: D000368:Aged; D001938:Brazil; D016022:Case-Control Studies; D002908:Chronic Disease; D005260:Female; D006801:Humans; D008297:Male; D053509:Matrix Metalloproteinase 13; D008875:Middle Aged; D057873:Peri-Implantitis / Q000235:genetics*; D010518:Periodontitis / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D019716:Tissue Inhibitor of Metalloproteinase-2; D053782:Transforming Growth Factor beta3
chemical_list: C560823:TIMP2 protein, human; D053782:Transforming Growth Factor beta3; D019716:Tissue Inhibitor of Metalloproteinase-2; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13