title: Prevalence and Sequence-Based Identity of Rumen Fluke in Cattle and Deer in New Caledonia.
abstract: An abattoir survey was performed in the French Melanesian archipelago of New Caledonia to determine the prevalence of paramphistomes in cattle and deer and to generate material for molecular typing at species and subspecies level. Prevalence in adult cattle was high at animal level (70% of 387 adult cattle) and batch level (81%). Prevalence was lower in calves at both levels (33% of 484 calves, 51% at batch level). Animals from 2 of 7 deer farms were positive for rumen fluke, with animal-level prevalence of 41.4% (29/70) and 47.1% (33/70), respectively. Using ITS-2 sequencing, 3 species of paramphistomes were identified, i.e. Calicophoron calicophorum, Fischoederius elongatus and Orthocoelium streptocoelium. All three species were detected in cattle as well as deer, suggesting the possibility of rumen fluke transmission between the two host species. Based on heterogeneity in ITS-2 sequences, the C. calicophorum population comprises two clades, both of which occur in cattle as well as deer. The results suggest two distinct routes of rumen fluke introduction into this area. This approach has wider applicability for investigations of the origin of rumen fluke infections and for the possibility of parasite transmission at the livestock-wildlife interface.
authors.affiliation: Institut Agronomique néo-Calédonien, Connaissance et amélioration des agrosystèmes, Laboratoire de Parasitologie, BP 73, 98890, Païta, Nouvelle Calédonie.
authors.forename: Laura
authors.initials: L
authors.lastname: Cauquil
authors.affiliation: Institut Agronomique néo-Calédonien, Connaissance et amélioration des agrosystèmes, Laboratoire de Parasitologie, BP 73, 98890, Païta, Nouvelle Calédonie.
authors.forename: Thomas
authors.initials: T
authors.lastname: Hüe
authors.affiliation: Institut Agronomique néo-Calédonien, Connaissance et amélioration des agrosystèmes, Laboratoire de Parasitologie, BP 73, 98890, Païta, Nouvelle Calédonie.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Hurlin
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Penicuik, EH26 0PZ, United Kingdom.
authors.forename: Gillian
authors.initials: G
authors.lastname: Mitchell
authors.affiliation: Centre for Ecology & Hydrology, Bush Estate, Penicuik, EH26 0QB, United Kingdom.
authors.forename: Kate
authors.initials: K
authors.lastname: Searle
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Penicuik, EH26 0PZ, United Kingdom.
authors.forename: Philip
authors.initials: P
authors.lastname: Skuce
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Penicuik, EH26 0PZ, United Kingdom.|Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, G61 1QH, United Kingdom.
authors.forename: Ruth
authors.initials: R
authors.lastname: Zadoks
mesh_terms: D000818:Animals; D002417:Cattle / Q000469:parasitology*; D002418:Cattle Diseases / Q000453:epidemiology / Q000235:genetics / Q000469:parasitology; D003670:Deer / Q000469:parasitology*; D009510:New Caledonia / Q000453:epidemiology; D010250:Paramphistomatidae / Q000235:genetics*; D014201:Trematode Infections / Q000453:epidemiology / Q000235:genetics