title: Fecal dysbiosis in miniature dachshunds with inflammatory colorectal polyps.
abstract: Chronic gastrointestinal disease is associated with the alteration of gastrointestinal microbiota. Inflammatory colorectal polyps (ICRPs) are commonly observed in miniature dachshunds (MDs) in Japan and are characterized by multiple polyps that are restricted in the colorectal mucosa with severe neutrophil infiltration. This study was aimed to compare the fecal microbiota of ICRP-affected MDs with that of healthy MDs. High-throughput sequencing of amplicons derived from the V3-V4 region of the 16S rRNA gene was applied using the Illumina MiSeq system. Principal coordinates analysis revealed that fecal microbiota of ICRP-affected MDs was significantly altered compared with that of healthy MDs. Proportions of Fusobacteriaceae, Helicobacteraceae, Porphyromonadaceae, and Turicibacteraceae were significantly more abundant in ICRP-affected MDs, while those of Lachnospiraceae were significantly less abundant in ICRP-affected MDs compared with healthy MDs. These results suggest that the dysbiosis is associated with ICRPs and is a potential therapeutic target, though further investigations are needed.
authors.affiliation: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Igarashi
authors.affiliation: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Electronic address: aohno@mail.ecc.u-tokyo.ac.jp.
authors.forename: Koichi
authors.initials: K
authors.lastname: Ohno
authors.affiliation: Food Science and Technology Institute, Morinaga Milk Industry Co. Ltd., Kanagawa, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Horigome
authors.affiliation: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Aki
authors.initials: A
authors.lastname: Fujiwara-Igarashi
authors.affiliation: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Kanemoto
authors.affiliation: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Fukushima
authors.affiliation: Food Science and Technology Institute, Morinaga Milk Industry Co. Ltd., Kanagawa, Japan.
authors.forename: Toshitaka
authors.initials: T
authors.lastname: Odamaki
authors.affiliation: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Tsujimoto
mesh_terms: D000818:Animals; D015179:Colorectal Neoplasms / Q000382:microbiology / Q000662:veterinary*; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D064806:Dysbiosis / Q000382:microbiology / Q000662:veterinary*; D005243:Feces / Q000382:microbiology*; D007249:Inflammation / Q000382:microbiology / Q000662:veterinary; D007564:Japan; D011127:Polyps / Q000382:microbiology / Q000662:veterinary*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA sequencing; Fecal microbiota; Inflammatory bowel disease; Inflammatory colorectal polyp; Miniature dachshund; Next generation sequencer