title: DNA catabolites in triathletes: effects of supplementation with an aronia-citrus juice (polyphenols-rich juice).
abstract: In this study we analyzed whether our aronia-citrus juice (ACJ, the composition is based on a mixture of 95% citrus juice with 5% of Aronia melanocarpa juice), rich in polyphenols, and physical exercise had an effect on seven catabolites of DNA identified in plasma and on a urine isoprostane (8-iso-PGF2α). Sixteen elite triathletes on a controlled diet for triathlon training (45 days) were used in this clinical trial. Our results show a decrease in the 8-hydroxy-2'-deoxyguanosine concentration due to chronic physical exercise. The ACJ intake and physical exercise maintained the guanosine-3',5'-cyclic monophosphate plasmatic concentrations and decreased the concentration of 8-hydroxyguanine as well as urinary values of 8-iso-PGF2α. Finally, we observed a significant increase in the 8-nitroguanosine levels in triathletes after ACJ intake, compared to the placebo stage. It is concluded that the combination of the intake of ACJ, rich in polyphenolic compounds, with adequate training was able to influence the plasmatic and urinary values of oxidative stress biomarkers. This suggests a positive effect on the oxidative damage and potential associations with DNA repair mechanisms.
authors.affiliation: Department of Food Science and Technology, CEBAS-CSIC, Campus de Espinardo 25, 30100 Espinardo, Murcia, Spain. angelgil@cebas.csic.es.
authors.forename: Libia Alejandra
authors.initials: LA
authors.lastname: García-Flores
authors.affiliation: Department of Food Science and Technology, CEBAS-CSIC, Campus de Espinardo 25, 30100 Espinardo, Murcia, Spain. angelgil@cebas.csic.es.
authors.forename: Sonia
authors.initials: S
authors.lastname: Medina
authors.affiliation: Department of Physical Education and Sport, Faculty of Education, University of Alicante, Campus San Vicente del Raspeig, 03540 San Vicent del Raspeig, Alicante, Spain.
authors.forename: Roberto
authors.initials: R
authors.lastname: Cejuela-Anta
authors.affiliation: Department of Physical Education and Sport, Faculty of Education, University of Alicante, Campus San Vicente del Raspeig, 03540 San Vicent del Raspeig, Alicante, Spain.
authors.forename: José Miguel
authors.initials: JM
authors.lastname: Martínez-Sanz
authors.affiliation: Department of Food Science and Technology, CEBAS-CSIC, Campus de Espinardo 25, 30100 Espinardo, Murcia, Spain. angelgil@cebas.csic.es.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Abellán
authors.affiliation: BIOLOG Life Science Institute, Flughafendamm 9a, 28199 Bremen, Germany.
authors.forename: Hans-Gottfried
authors.initials: HG
authors.lastname: Genieser
authors.affiliation: Department of Food Science and Technology, CEBAS-CSIC, Campus de Espinardo 25, 30100 Espinardo, Murcia, Spain. angelgil@cebas.csic.es.
authors.forename: Federico
authors.initials: F
authors.lastname: Ferreres
authors.affiliation: Department of Food Science and Technology, CEBAS-CSIC, Campus de Espinardo 25, 30100 Espinardo, Murcia, Spain. angelgil@cebas.csic.es.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Gil-Izquierdo
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D056352:Athletes; D001628:Beverages / Q000032:analysis*; D002957:Citrus / Q000737:chemistry / Q000378:metabolism*; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D004032:Diet; D005260:Female; D006801:Humans; D008297:Male; D018384:Oxidative Stress; D046936:Photinia / Q000737:chemistry / Q000378:metabolism*; D005082:Physical Exertion; D059808:Polyphenols / Q000378:metabolism*; D055815:Young Adult
chemical_list: D059808:Polyphenols; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine