title: Measuring the Composition and Stable-Isotope Labeling of Algal Biomass Carbohydrates via Gas Chromatography/Mass Spectrometry.
abstract: We have developed a method to measure carbohydrate composition and stable-isotope labeling in algal biomass using gas chromatography/mass spectrometry (GC/MS). The method consists of two-stage hydrochloric acid hydrolysis, followed by chemical derivatization of the released monomer sugars and quantification by GC/MS. Fully (13)C-labeled sugars are used as internal standards for composition analysis. This convenient, reliable, and accurate single-platform workflow offers advantages over existing methods and opens new opportunities to study carbohydrate metabolism of algae under autotrophic, mixotrophic, and heterotrophic conditions using metabolic flux analysis and isotopic tracers such as (2)H2O and (13)C-glucose.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Metabolic Engineering and Systems Biology Laboratory, University of Delaware , Newark, Delaware 19716, United States.
authors.forename: Brian O
authors.initials: BO
authors.lastname: McConnell
authors.affiliation: Department of Chemical and Biomolecular Engineering, Metabolic Engineering and Systems Biology Laboratory, University of Delaware , Newark, Delaware 19716, United States.
authors.forename: Maciek R
authors.initials: MR
authors.lastname: Antoniewicz
mesh_terms: D018533:Biomass; D002241:Carbohydrates / Q000032:analysis*; D002247:Carbon Isotopes / Q000032:analysis*; D048408:Chlorella vulgaris / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D007553:Isotope Labeling
chemical_list: D002241:Carbohydrates; D002247:Carbon Isotopes