title: Expression of Estrogen Receptor Coactivator Proline-, Glutamic Acid- and Leucine-Rich Protein 1 within Paraspinal Muscles in Adolescents with Idiopathic Scoliosis.
abstract: OBJECTIVE
  The aim of this study was to detect and assess the estrogen receptor (ESR) coactivator PELP1 expression within human paraspinal skeletal muscles in patients suffering from idiopathic scoliosis.
  
  
  METHODS
  During surgical correction of scoliosis the muscle biopsies harvested in 29 females. Presence of PELP1, ESR1 and ESR2 genes transcripts was studied using RT-qPCR technique while immunohistochemistry and western blot methods were used to detect the PEPL1 protein presence.
  
  
  RESULTS
  PELP1 expression in deep paraspinal muscles revealed higher than in superficial back muscles (p = 0.005). Positive immunohistochemical staining for PELP1 was observed in the nuclei of the paraspinal muscle cells. Western blot revealed PELP1 protein in all samples. No significant difference in PELP1 expression between the convex and the concave scoliosis side (p>0.05) was found. In deep paraspinal back muscles, a significant correlation between the PELP1 expression level on the concave side and the Cobb angle (r = 0.4; p<0.05) was noted as well as between the PELP1 and ESR1 expression level (r = 0.7; p<0.05) while no correlation between PELP1 and ESR2 expression level was found.
  
  
  CONCLUSIONS
  To our knowledge, three techniques for the first time demonstrated the presence of the PELP1 in paraspinal muscles of patients with idiopathic scoliosis. The PELP1 potential regulatory impact on back muscle function is to be further investigated.
authors.affiliation: Department of Cell Biology, Health Sciences Faculty, University of Medical Sciences, Poznan, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Skibinska
authors.affiliation: Spine Disorders and Pediatric Orthopedics Department, Faculty of Medicine I, University of Medical Sciences, Poznan, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Tomaszewski
authors.affiliation: Department of Cell Biology, Health Sciences Faculty, University of Medical Sciences, Poznan, Poland.
authors.forename: Miroslaw
authors.initials: M
authors.lastname: Andrusiewicz
authors.affiliation: Department of Cell Biology, Health Sciences Faculty, University of Medical Sciences, Poznan, Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Urbaniak
authors.affiliation: Department of Cell Biology, Health Sciences Faculty, University of Medical Sciences, Poznan, Poland.
authors.forename: Roza
authors.initials: R
authors.lastname: Czarnecka-Klos
authors.affiliation: Spine Disorders and Pediatric Orthopedics Department, Faculty of Medicine I, University of Medical Sciences, Poznan, Poland.
authors.forename: Milud
authors.initials: M
authors.lastname: Shadi
authors.affiliation: Spine Disorders and Pediatric Orthopedics Department, Faculty of Medicine I, University of Medical Sciences, Poznan, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Kotwicki
authors.affiliation: Department of Cell Biology, Health Sciences Faculty, University of Medical Sciences, Poznan, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Kotwicka
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D056970:Co-Repressor Proteins / Q000096:biosynthesis*; D047628:Estrogen Receptor alpha / Q000096:biosynthesis*; D047629:Estrogen Receptor beta / Q000096:biosynthesis*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology; D012600:Scoliosis / Q000378:metabolism* / Q000473:pathology; D014157:Transcription Factors / Q000096:biosynthesis*
chemical_list: D056970:Co-Repressor Proteins; C506487:ESR1 protein, human; C000600034:ESR2 protein, human; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; C438485:PELP1 protein, human; D014157:Transcription Factors