title: Detecting PTEN and PI3K Signaling in Brain.
abstract: The central nervous system is comprised of multiple cell types including neurons, glia, and other supporting cells that may differ dramatically in levels of signaling pathway activation. Immunohistochemistry in conjunction with drug interference are powerful tools that allow evaluation of signaling pathways in different cell types of the mouse central nervous system in vivo. Here we provide detailed protocols for immunohistochemistry to evaluate three essential components in the PI3K pathway in mouse brain: Pten, p-Akt, and p-4ebp1, and for rapamycin treatment to modulate mTOR signaling in vivo.
authors.affiliation: Department of Pathology, University of Tennessee Health Sciences Center, Memphis, TN, 38163, USA.
authors.forename: Guo
authors.initials: G
authors.lastname: Zhu
authors.affiliation: Department of Developmental Neurobiology, St Jude Children's Research Hospital, Room D2006C, 262 Danny Thomas Place, Memphis, TN, 38105-3678, USA. suzanne.baker@stjude.org.
authors.forename: Suzanne J
authors.initials: SJ
authors.lastname: Baker
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods*; D051379:Mice; D051059:PTEN Phosphohydrolase / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D015398:Signal Transduction; D020123:Sirolimus / Q000008:administration & dosage
chemical_list: D019869:Phosphatidylinositol 3-Kinases; D051059:PTEN Phosphohydrolase; C487757:Pten protein, mouse; D020123:Sirolimus
keywords: Immunohistochemistry; PI3K; PTEN; Rapamycin; p-4EBP1; p-AKT