title: ITPI: Initial Transcription Process-Based Identification Method of Bioactive Components in Traditional Chinese Medicine Formula.
abstract: Identification of bioactive components is an important area of research in traditional Chinese medicine (TCM) formula. The reported identification methods only consider the interaction between the components and the target proteins, which is not sufficient to explain the influence of TCM on the gene expression. Here, we propose the Initial Transcription Process-based Identification (ITPI) method for the discovery of bioactive components that influence transcription factors (TFs). In this method, genome-wide chip detection technology was used to identify differentially expressed genes (DEGs). The TFs of DEGs were derived from GeneCards. The components influencing the TFs were derived from STITCH. The bioactive components in the formula were identified by evaluating the molecular similarity between the components in formula and the components that influence the TF of DEGs. Using the formula of Tian-Zhu-San (TZS) as an example, the reliability and limitation of ITPI were examined and 16 bioactive components that influence TFs were identified.
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Baixia
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Institute of Information on Traditional Chinese Medicine of China, Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Yanwen
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: China Minority Traditional Medical Center, Minzu University of China, Beijing 100081, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Li
authors.affiliation: China Minority Traditional Medical Center, Minzu University of China, Beijing 100081, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Bi
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Yusu
authors.initials: Y
authors.lastname: He
authors.affiliation: China Minority Traditional Medical Center, Minzu University of China, Beijing 100081, China.
authors.forename: Kuokui
authors.initials: K
authors.lastname: Song
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang