title: A nutrient mixture inhibits glioblastoma xenograft U-87 MG growth in male nude mice.
abstract: BACKGROUND
  Brain tumors are highly aggressive tumors characterized by secretions of high levels of matrix metalloproteinase-2 and -9, leading to tumor growth, invasion and metastasis by digesting the basement membrane and extracellular matrix components. We previously demonstrated the effectiveness of a nutrient mixture (NM) containing ascorbic acid, lysine, proline, and green tea extract in vitro: on activity of urokinase plasminogen activator, matrix metalloproteinases and TIMPs in various human glioblastoma (LN-18, T-98G and A-172) cell lines and on glioblastoma A-172 cell proliferation and Matrigel invasion.
  
  
  OBJECTIVE
  Our main objective in this study was to investigate the effect of the NM in vivo on human glioblastoma U-87 MG cell line.
  
  
  METHODS
  Athymic male nude mice inoculated with 3·10(6) U-87 MG cells subcutaneously and were fed a regular diet or a regular diet supplemented with 0.5% NM. Four weeks later, the mice were sacrificed, the tumors were weighed and measured. The samples were studied histologically.
  
  
  RESULTS
  NM inhibited tumor weight and tumor burden by 53% (p = 0.015) and 48% (p = 0.010), respectively.
  
  
  CONCLUSIONS
  These results suggest the therapeutic potential of NM as an adjuvant in the treatment of glioblastoma.
authors.affiliation: Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050, USA.
authors.forename: M W
authors.initials: MW
authors.lastname: Roomi
authors.affiliation: Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050, USA.
authors.forename: T
authors.initials: T
authors.lastname: Kalinovsky
authors.affiliation: Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050, USA.
authors.forename: M
authors.initials: M
authors.lastname: Rath
authors.affiliation: Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050, USA.
authors.forename: A
authors.initials: A
authors.lastname: Niedzwiecki
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis / Q000627:therapeutic use*; D001921:Brain / Q000473:pathology*; D001932:Brain Neoplasms / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D019587:Dietary Supplements / Q000032:analysis; D005909:Glioblastoma / Q000473:pathology / Q000628:therapy*; D006801:Humans; D008239:Lysine / Q000032:analysis / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D011392:Proline / Q000032:analysis / Q000627:therapeutic use*; D013662:Tea / Q000737:chemistry
chemical_list: D013662:Tea; D011392:Proline; D008239:Lysine; D001205:Ascorbic Acid