title: Selective plane illumination microscopy on a chip.
abstract: Selective plane illumination microscopy can image biological samples at a high spatiotemporal resolution. Complex sample preparation and system alignment normally limit the throughput of the method. Using femtosecond laser micromachining, we created an integrated optofluidic device that allows obtaining continuous flow imaging, three-dimensional reconstruction and high-throughput analysis of large multicellular spheroids at a subcellular resolution.
authors.affiliation: Dipartimento di Fisica, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy. andreabassi@polimi.it and Istituto di Fotonica e Nanotecnologie, CNR, Piazza Leonardo da Vinci 32, 20133 Milano, Italy.
authors.forename: Petra
authors.initials: P
authors.lastname: Paiè
authors.affiliation: Istituto di Fotonica e Nanotecnologie, CNR, Piazza Leonardo da Vinci 32, 20133 Milano, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Bragheri
authors.affiliation: Dipartimento di Fisica, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy. andreabassi@polimi.it.
authors.forename: Andrea
authors.initials: A
authors.lastname: Bassi
authors.affiliation: Dipartimento di Fisica, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy. andreabassi@polimi.it and Istituto di Fotonica e Nanotecnologie, CNR, Piazza Leonardo da Vinci 32, 20133 Milano, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Osellame
mesh_terms: D000818:Animals; D004867:Equipment Design; D057166:High-Throughput Screening Assays / Q000295:instrumentation*; D006657:Histones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000295:instrumentation*; D056656:Lab-On-A-Chip Devices; D008164:Luminescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D059010:Single-Cell Analysis / Q000295:instrumentation; D018874:Spheroids, Cellular / Q000166:cytology; D000097573:Red Fluorescent Protein
chemical_list: D006657:Histones; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D000097573:Red Fluorescent Protein