title: Three-dimensional culture and interaction of cancer cells and dendritic cells in an electrospun nano-submicron hybrid fibrous scaffold.
abstract: An artificial three-dimensional (3D) culture system that mimics the tumor microenvironment in vitro is an essential tool for investigating the cross-talk between immune and cancer cells in tumors. In this study, we developed a 3D culture system using an electrospun poly(ε-caprolactone) (PCL) nanofibrous scaffold (NFS). A hybrid NFS containing an uninterrupted network of nano- and submicron-scale fibers (400 nm to 2 µm) was generated by deposition onto a stainless steel mesh instead of an aluminum plate. The hybrid NFS contained multiplanar pores in a 3D structure. Surface-seeded mouse CT26 colon cancer cells and bone marrow-derived dendritic cells (BM-DCs) were able to infiltrate the hybrid NFS within several hours. BM-DCs cultured on PCL nanofibers showed a baseline inactive form, and lipopolysaccharide (LPS)-activated BM-DCs showed increased expression of CD86 and major histocompatibility complex Class II. Actin and phosphorylated FAK were enriched where unstimulated and LPS-stimulated BM-DCs contacted the fibers in the 3D hybrid NFS. When BM-DCs were cocultured with mitoxantrone-treated CT26 cells in a 3D hybrid NFS, BM-DCs sprouted cytoplasm to, migrated to, synapsed with, and engulfed mitoxantrone-treated CT26 cancer cells, which were similar to the naturally occurring cross-talk between these two types of cells. The 3D hybrid NFS developed here provides a 3D structure for coculture of cancer and immune cells.
authors.affiliation: Department of Pharmacology, School of Medicine, Ajou University, Suwon, South Korea; Department of Biomedical Sciences, The Graduate School, Ajou University, Suwon, South Korea; Immune Network Pioneer Research Center, Ajou University Medical Center, Suwon, South Korea.
authors.forename: Tae-Eon
authors.initials: TE
authors.lastname: Kim
authors.affiliation: Department of Pharmacology, School of Medicine, Ajou University, Suwon, South Korea; Department of Biomedical Sciences, The Graduate School, Ajou University, Suwon, South Korea; Immune Network Pioneer Research Center, Ajou University Medical Center, Suwon, South Korea.
authors.forename: Chang Gun
authors.initials: CG
authors.lastname: Kim
authors.affiliation: Department of Mechanical Engineering, Korea Polytechnic University, Gyeonggi, South Korea.
authors.forename: Jin Soo
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Department of Mechanical Engineering, Korea Polytechnic University, Gyeonggi, South Korea.
authors.forename: Songwan
authors.initials: S
authors.lastname: Jin
authors.affiliation: Department of Anatomy, School of Medicine, Pusan National University, Yangsan, South Korea.
authors.forename: Sik
authors.initials: S
authors.lastname: Yoon
authors.affiliation: Department of Physiology, College of Medicine, Dong-A University, Busan, South Korea.
authors.forename: Hae-Rahn
authors.initials: HR
authors.lastname: Bae
authors.affiliation: School of Mechanical Engineering, Kyungpook National University, Daegu, South Korea; Department of Mechanical Engineering, Graduate School, Kyungpook National University, Daegu, South Korea.
authors.forename: Jeong-Hwa
authors.initials: JH
authors.lastname: Kim
authors.affiliation: School of Mechanical Engineering, Kyungpook National University, Daegu, South Korea; Department of Mechanical Engineering, Graduate School, Kyungpook National University, Daegu, South Korea.
authors.forename: Young Hun
authors.initials: YH
authors.lastname: Jeong
authors.affiliation: Department of Pharmacology, School of Medicine, Ajou University, Suwon, South Korea; Department of Biomedical Sciences, The Graduate School, Ajou University, Suwon, South Korea; Immune Network Pioneer Research Center, Ajou University Medical Center, Suwon, South Korea.
authors.forename: Jong-Young
authors.initials: JY
authors.lastname: Kwak
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001853:Bone Marrow / Q000254:growth & development*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D003110:Colonic Neoplasms / Q000473:pathology*; D003713:Dendritic Cells / Q000166:cytology*; D004563:Electrochemistry / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C062968:lactide-caprolactone copolymer
keywords: 3D cell culture; colon cancer cell; dendritic cell; electrospinning; nanofibrous scaffold