title: Evaluation of a bilayered, micropatterned hydrogel dressing for full-thickness wound healing.
abstract: Nearly 12 million wounds are treated in emergency departments throughout the United States every year. The limitations of current treatments for complex, full-thickness wounds are the driving force for the development of new wound treatment devices that result in faster healing of both dermal and epidermal tissue. Here, a bilayered, biodegradable hydrogel dressing that uses microarchitecture to guide two key steps in the proliferative phase of wound healing, re-epithelialization, and revascularization, was evaluated in vitro in a cell migration assay and in vivo in a bipedicle ischemic rat wound model. Results indicate that the Sharklet™-micropatterned apical layer of the dressing increased artificial wound coverage by up to 64%, P = 0.024 in vitro. In vivo evaluation demonstrated that the bilayered dressing construction enhanced overall healing outcomes significantly compared to untreated wounds and that these outcomes were not significantly different from a leading clinically available wound dressing. Collectively, these results demonstrate high potential for this new dressing to effectively accelerate wound healing.
authors.affiliation: Sharklet Technologies, Inc., Aurora, CO 80045, USA cmagin@sharklet.com.
authors.forename: Chelsea M
authors.initials: CM
authors.lastname: Magin
authors.affiliation: Department of Materials Science and Engineering, University of Florida, Gainesville, FL 32611, USA.
authors.forename: Dylan B
authors.initials: DB
authors.lastname: Neale
authors.affiliation: Sharklet Technologies, Inc., Aurora, CO 80045, USA.
authors.forename: Michael C
authors.initials: MC
authors.lastname: Drinker
authors.affiliation: Department of Materials Science and Engineering, University of Florida, Gainesville, FL 32611, USA Department of Internal Medicine, University of Central Florida, Orlando, FL 32827, USA Saisijin Biotech, LLC, Orlando, FL 32827, USA.
authors.forename: Bradley J
authors.initials: BJ
authors.lastname: Willenberg
authors.affiliation: Sharklet Technologies, Inc., Aurora, CO 80045, USA.
authors.forename: Shravanthi T
authors.initials: ST
authors.lastname: Reddy
authors.affiliation: Department of Veterinary Biosciences, College of Veterinary Medicine and Comparative Pathology & Mouse Phenotyping Shared Resource, Comprehensive Cancer Center The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Krista Md
authors.initials: KM
authors.lastname: La Perle
authors.affiliation: Department of Materials Science and Engineering, University of Florida, Gainesville, FL 32611, USA.
authors.forename: Gregory S
authors.initials: GS
authors.lastname: Schultz
authors.affiliation: Sharklet Technologies, Inc., Aurora, CO 80045, USA Saisijin Biotech, LLC, Orlando, FL 32827, USA J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL 32611, USA.
authors.forename: Anthony B
authors.initials: AB
authors.lastname: Brennan
mesh_terms: D000818:Animals; D001458:Bandages; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D002465:Cell Movement; D006801:Humans; D015603:Keratinocytes / Q000166:cytology; D008297:Male; D008422:Materials Testing; D017207:Rats, Sprague-Dawley; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials
keywords: Micropattern; biomaterials; gelatin; hydrogel; microarchitecture; wound healing