title: Protective Effects of Paeoniflorin Against MPP(+)-induced Neurotoxicity in PC12 Cells.
abstract: In the present study, we investigated the protective mechanism of paeoniflorin (PF), a monoterpene glycoside extracted from Radix Paeoniae alba roots, on MPP(+)-induced neurotoxicity in cultured rat pheochromocytoma cells (PC12). Our work included examination of cell viability assessment, amounts of released lactic dehydrogenase (LDH), intracellular Ca(2+) concentration, cell apoptosis, mitochondrial membrane potential, caspase-3 activity, and expression profiling of two apoptosis-related genes (Bcl-2 and Bax). It was shown that, PF functioned as an MPP(+) antagonist, being able to suppress apoptosis, decrease LDH release and Ca(2+) concentration, attenuate membrane potential collapse and, inhibit caspase-3 activation, decrease in Bax/Bcl-2 ratio. These observations suggest that PF could protect PC12 cells against MPP(+)-induced injury and the mechanism PF's neuroprotective effect was closely associated with Bcl-2 up-regulation and Bax down-regulation. PF has neuroprotective effects on MPP(+)-induced apoptosis in PC12 cells via regulating mitochondrial membrane potential and Bcl-2/Bax/caspase-3 signaling pathways, and this new insight will help develop a PF-based therapeutic strategy for treatmenting neurodegenerative diseases and injury.
authors.affiliation: The Central Laboratory, Changchun Normal University, Changchun, 130032, Jilin, China.
authors.forename: Meizhu
authors.initials: M
authors.lastname: Zheng
authors.affiliation: The Central Laboratory, Changchun Normal University, Changchun, 130032, Jilin, China. ccsfxy777@163.com.
authors.forename: Chunming
authors.initials: C
authors.lastname: Liu
authors.affiliation: College of Life Science, Changchun Normal University, Changchun, 130032, Jilin, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Fan
authors.affiliation: The Central Laboratory, Changchun Normal University, Changchun, 130032, Jilin, China.
authors.forename: Dongfang
authors.initials: D
authors.lastname: Shi
authors.affiliation: The Central Laboratory, Changchun Normal University, Changchun, 130032, Jilin, China.
authors.forename: Yuchi
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D015655:1-Methyl-4-phenylpyridinium / Q000633:toxicity*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D005960:Glucosides / Q000494:pharmacology*; D039821:Monoterpenes / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016716:PC12 Cells; D051381:Rats; D006027:Glycosides
chemical_list: D015655:1-Methyl-4-phenylpyridinium; D005960:Glucosides; D039821:Monoterpenes; D018696:Neuroprotective Agents; C015423:peoniflorin; D006027:Glycosides
keywords: Neuroprotective effect; PC12 cells; Paeoniflorin; Parkinson’s disease