title: Distinct temporal requirements for autophagy and the proteasome in yeast meiosis.
abstract: Meiosis is a special type of cellular renovation that involves 2 successive cell divisions and a single round of DNA replication. Two major degradation systems, the autophagy-lysosome and the ubiquitin-proteasome, are involved in meiosis, but their roles have yet to be elucidated. Here we show that autophagy mainly affects the initiation of meiosis but not the nuclear division. Autophagy works not only by serving as a dynamic recycling system but also by eliminating some negative meiotic regulators such as Ego4 (Ynr034w-a). In a quantitative proteomics study, the proteasome was found to be significantly upregulated during meiotic divisions. We found that proteasomal activity is essential to the 2 successive meiotic nuclear divisions but not for the initiation of meiosis. Our study defines the roles of autophagy and the proteasome in meiosis: Autophagy mainly affects the initiation of meiosis, whereas the proteasome mainly affects the 2 successive meiotic divisions.
authors.affiliation: a State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing , China.|c University of Chinese Academy of Sciences , Beijing , China.
authors.forename: Fu-ping
authors.initials: FP
authors.lastname: Wen
authors.affiliation: b State Key Laboratory of Reproductive Medicine, Collaborative Innovation Center of Genetics and Development , Department of Histology and Embryology , Nanjing Medical University , Nanjing , Jiangsu , China.
authors.forename: Yue-shuai
authors.initials: YS
authors.lastname: Guo
authors.affiliation: a State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing , China.|d College of Life Sciences, China West Normal University , Nanchong , China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Hu
authors.affiliation: a State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing , China.
authors.forename: Wei-xiao
authors.initials: WX
authors.lastname: Liu
authors.affiliation: a State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing , China.|c University of Chinese Academy of Sciences , Beijing , China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: a State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing , China.|c University of Chinese Academy of Sciences , Beijing , China.
authors.forename: Yuan-ting
authors.initials: YT
authors.lastname: Wang
authors.affiliation: a State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing , China.|c University of Chinese Academy of Sciences , Beijing , China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Yu
authors.affiliation: a State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing , China.|c University of Chinese Academy of Sciences , Beijing , China.
authors.forename: Chao-ming
authors.initials: CM
authors.lastname: Tang
authors.affiliation: d College of Life Sciences, China West Normal University , Nanchong , China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.affiliation: b State Key Laboratory of Reproductive Medicine, Collaborative Innovation Center of Genetics and Development , Department of Histology and Embryology , Nanjing Medical University , Nanjing , Jiangsu , China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.affiliation: e School of Life Sciences and Biotechnology, Shanghai Jiao Tong University , Shanghai , China.
authors.forename: Zhi-ping
authors.initials: ZP
authors.lastname: Xie
authors.affiliation: b State Key Laboratory of Reproductive Medicine, Collaborative Innovation Center of Genetics and Development , Department of Histology and Embryology , Nanjing Medical University , Nanjing , Jiangsu , China.
authors.forename: Jia-hao
authors.initials: JH
authors.lastname: Sha
authors.affiliation: b State Key Laboratory of Reproductive Medicine, Collaborative Innovation Center of Genetics and Development , Department of Histology and Embryology , Nanjing Medical University , Nanjing , Jiangsu , China.
authors.forename: Xuejiang
authors.initials: X
authors.lastname: Guo
authors.affiliation: a State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing , China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
mesh_terms: D001343:Autophagy; D004261:DNA Replication; D015536:Down-Regulation; D008540:Meiosis; D049471:Pachytene Stage; D011418:Prophase; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D011485:Protein Binding; D040901:Proteomics; D015203:Reproducibility of Results; D012270:Ribosomes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D013997:Time Factors
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D046988:Proteasome Endopeptidase Complex
keywords: autophagy; meiosis; proteasome; quantitative proteomics; yeast