title: Differential effects of short co-incubation of gametes and early removal of cumulus cells in patients with different fertilizing capabilities.
abstract: The objective of this retrospective study was to evaluate the embryological and clinical outcomes of short co-incubation of gametes and early removal of cumulus cells (SCGERCC) in patients with good IVF capability and those with complete fertilization failure treated by rescue intracytoplasmic sperm injection (ICSI). The study included 257 couples with >60% fertilization rate (SCGERCC group) in the SCGERCC cycle, 72 couples with complete fertilization failure in the SCGERCC cycle given early rescue ICSI treatment (early rescue group), and 219 couples who underwent IVF cycles with overnight co-incubation of gametes with >60% fertilization rate (traditional IVF group). The results showed that the SCGERCC group had a higher multi-pronuclei rate (13.34%, P < 0.001) than the early rescue ICSI (5.15%) and traditional IVF (6.15%) groups. The good quality embryo rate was higher in the SCGERCC group, but implantation, clinical pregnancy and live-birth rates (37.21%, 36.92% and 38.20% for SCGERCC, early rescue and traditional IVF groups, respectively) were comparable in all three groups. The study indicated that SCGERCC followed by rescue ICSI helped couples with initial complete fertilization failure attain clinical outcomes comparable with the other two groups, but it significantly increased the multi-pronuclei rate in couples with good fertilizing capabilities.
authors.affiliation: Department of Reproductivke Medicine, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Reproductivke Medicine, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Reproductivke Medicine, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Department of Reproductivke Medicine, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Reproductivke Medicine, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Reproductivke Medicine, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Geng
authors.affiliation: Department of Reproductivke Medicine, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.
authors.forename: Guiqin
authors.initials: G
authors.lastname: Hao
authors.affiliation: Department of Reproductivke Medicine, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Reproductivke Medicine, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Reproductivke Medicine, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China. Electronic address: QIANWP2014@163.com.
authors.forename: Weiping
authors.initials: W
authors.lastname: Qian
mesh_terms: D000328:Adult; D002467:Cell Nucleus / Q000378:metabolism; D054885:Cumulus Cells / Q000166:cytology*; D010064:Embryo Implantation; D004624:Embryo Transfer; D005260:Female; D005306:Fertilization; D005307:Fertilization in Vitro / Q000379:methods*; D005854:Germ Cells / Q000166:cytology*; D006728:Hormones / Q000378:metabolism; D006801:Humans; D007246:Infertility / Q000628:therapy*; D008297:Male; D009865:Oocytes / Q000166:cytology; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods; D015053:Zygote
chemical_list: D006728:Hormones
keywords: ICSI; IVF; early removal of cumulus cells; early rescue; multi-pronuclei; short co-incubation of gametes