title: The role of inositol 1,4,5-trisphosphate 3-kinase A in regulating emotional behavior and amygdala function.
abstract: Inositol 1,4,5-trisphosphate 3-kinase A (IP3K-A) is a molecule enriched in the brain and neurons that regulates intracellular calcium levels via signaling through the inositol trisphosphate receptor. In the present study, we found that IP3K-A expression is highly enriched in the central nucleus of the amygdala (CeA), which plays a pivotal role in the processing and expression of emotional phenotypes in mammals. Genetic abrogation of IP3K-A altered amygdala gene expression, particularly in genes involved in key intracellular signaling pathways and genes mediating fear- and anxiety-related behaviors. In agreement with the changes in amygdala gene expression profiles, IP3K-A knockout (KO) mice displayed more robust responses to aversive stimuli and spent less time in the open arms of the elevated plus maze, indicating high levels of innate fear and anxiety. In addition to behavioral phenotypes, decreased excitatory and inhibitory postsynaptic current and reduced c-Fos immunoreactivity in the CeA of IP3K-A KO mice suggest that IP3K-A has a profound influence on the basal activities of fear- and anxiety-mediating amygdala circuitry. In conclusion, our findings collectively demonstrate that IP3K-A plays an important role in regulating affective states by modulating metabotropic receptor signaling pathways and neural activity in the amygdala.
authors.affiliation: Department of Biomedical Sciences, Brain Korea 21 PLUS, College of Medicine, Korea University, Seoul 136-705, Korea.|Department of Brain and Cognitive Sciences, Scranton College, Ewha Womans University, Seoul, 03760, Korea.
authors.forename: Sooyoung
authors.initials: S
authors.lastname: Chung
authors.affiliation: Department of Cell Biology, Duke University Medical School, Durham, North Carolina 27710, USA.
authors.forename: Il Hwan
authors.initials: IH
authors.lastname: Kim
authors.affiliation: Department of Anatomy, College of Medicine, Korea University, Seoul 136-705, Korea.
authors.forename: Dongmin
authors.initials: D
authors.lastname: Lee
authors.affiliation: Department of Biology, Department of Life and Nanopharmaceutical Sciences, Kyunghee University, Seoul 130-701, Korea.
authors.forename: Kyungjoon
authors.initials: K
authors.lastname: Park
authors.affiliation: Department of Biomedical Sciences, Brain Korea 21 PLUS, College of Medicine, Korea University, Seoul 136-705, Korea.|Department of Anatomy, College of Medicine, Korea University, Seoul 136-705, Korea.
authors.forename: Joo Yeon
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Department of Psychology, Korea University, Seoul 136-701, Korea.
authors.forename: Yeon Kyung
authors.initials: YK
authors.lastname: Lee
authors.affiliation: Department of Psychology, University of Washington, Seattle, WA, USA.
authors.forename: Eun Joo
authors.initials: EJ
authors.lastname: Kim
authors.affiliation: Department of Biomedical Sciences, Brain Korea 21 PLUS, College of Medicine, Korea University, Seoul 136-705, Korea.|Department of Anatomy, College of Medicine, Korea University, Seoul 136-705, Korea.
authors.forename: Hyun Woo
authors.initials: HW
authors.lastname: Lee
authors.affiliation: Department of Psychology, Korea University, Seoul 136-701, Korea.
authors.forename: June-seek
authors.initials: JS
authors.lastname: Choi
authors.affiliation: Department of Biomedical Sciences, Brain Korea 21 PLUS, College of Medicine, Korea University, Seoul 136-705, Korea.
authors.forename: Gi Hoon
authors.initials: GH
authors.lastname: Son
authors.affiliation: Department of Biomedical Sciences, Brain Korea 21 PLUS, College of Medicine, Korea University, Seoul 136-705, Korea.|Department of Anatomy, College of Medicine, Korea University, Seoul 136-705, Korea.
authors.forename: Woong
authors.initials: W
authors.lastname: Sun
authors.affiliation: Department of Psychology, Korea University, Seoul 136-701, Korea.
authors.forename: Ki Soon
authors.initials: KS
authors.lastname: Shin
authors.affiliation: Department of Biomedical Sciences, Brain Korea 21 PLUS, College of Medicine, Korea University, Seoul 136-705, Korea.|Department of Anatomy, College of Medicine, Korea University, Seoul 136-705, Korea.
authors.forename: Hyun
authors.initials: H
authors.lastname: Kim
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D066274:Central Amygdaloid Nucleus / Q000378:metabolism / Q000502:physiology*; D004644:Emotions; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics* / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D017853:Phosphotransferases (Alcohol Group Acceptor); C051038:Inositol 1,4,5-trisphosphate 3-kinase