title: MicroRNA-130a promotes the metastasis and epithelial-mesenchymal transition of osteosarcoma by targeting PTEN.
abstract: MicroRNAs, which serve as post-transcriptional modulators of numerous genes, have been found to be important regulators during the pathogenesis of osteosarcoma. This study demonstrates for the first time that microRNA-130a (miR-130a) is significantly upregulated in osteosarcoma, and associated with the metastasis of osteosarcoma. Elevated level of miR-130a was closely correlated with poor clinical features and prognosis of osteosarcoma patients. In vitro assays revealed that miR-130a could potentiate the migration, invasion and the epithelial-mesenchymal transtion (EMT) of osteosarcoma cells. Moreover, phosphatase and tensin homolog (PTEN) was confirmed as not only a direct downstream target but also a functional mediator of miR-130a. MiR-130a exerted promoting effects on metastatic behavior and EMT of osteosarcoma cells through suppressing PTEN expression. Based on these findings, we conclude that miR-130a is a promising prognostic biomarker for osteosarcoma patients, and targeting miR-130a may be a potential treatment option for osteosarcoma patients with metastasis.
authors.affiliation: Department of Orthopaedics, Children's Hospital of Zhejiang University, School of Medicine, Hangzhou, Zhejiang, P.R. China.
authors.forename: Jiansong
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Gynecologic Oncology, Zhejiang Cancer Hospital, Hangzhou, Zhejiang, P.R. China.
authors.forename: Dingding
authors.initials: D
authors.lastname: Yan
authors.affiliation: Department of Orthopaedics, Children's Hospital of Zhejiang University, School of Medicine, Hangzhou, Zhejiang, P.R. China.
authors.forename: Weiliang
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Orthopaedics, Children's Hospital of Zhejiang University, School of Medicine, Hangzhou, Zhejiang, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Orthopaedics, Children's Hospital of Zhejiang University, School of Medicine, Hangzhou, Zhejiang, P.R. China.
authors.forename: Wensong
authors.initials: W
authors.lastname: Ye
authors.affiliation: Department of Tumor Surgery, Children's Hospital of Zhejiang University, School of Medicine, Hangzhou, Zhejiang, P.R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Shu
mesh_terms: D020413:3' Untranslated Regions; D000328:Adult; D000704:Analysis of Variance; D001483:Base Sequence; D001665:Binding Sites; D001859:Bone Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D012516:Osteosarcoma / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000556:secondary; D051059:PTEN Phosphohydrolase / Q000235:genetics* / Q000378:metabolism; D011379:Prognosis; D055815:Young Adult
chemical_list: D020413:3' Untranslated Regions; C554019:MIRN130 microRNA, human; D035683:MicroRNAs; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human