title: Biomimetic Mineralization of the Alginate/Gelatin/Calcium Oxalate Matrix for Immobilization of Pectinase: Influence of Matrix on the Pectinolytic Activity.
abstract: Pectinases catalyze the degradation of pectic substances and are used in several processes, mainly in food and textile industries. In this study, a biomimetic matrix of alginate/gelatin/calcium oxalate (AGOCa) was synthesized for the in situ immobilization via encapsulation of crude pectinase from Aspergillus niger ATCC 9642, obtaining an immobilization efficiency of about 61.7 %. To determine the performance of AGOCa matrix, this was compared to control matrices of alginate/calcium oxalate (AOxal) and alginate/water (ACa). By the evaluation of pH and temperature effects on the enzyme activity, it was observed an increase on pectinolytic activity for both three tested matrices with an increase on pH and temperature. The kinetic parameters for pectinase immobilized in the three matrices were determined using citric pectin as substrate. Values of K m of 0.003, 0.0013, and 0.0022 g mL(-1) and V max of 3.85, 4.32, and 3.17 μmol min(-1) g(-1) for AGOCa, AOxal, and ACa matrices were obtained, respectively. After 33 days of storage, the pectinase immobilized in the three different matrices kept its initial activity, but that immobilized in AGOCa presented high stability to the storage with a relative activity of about 160 %. The enzyme immobilized in AGOCa, AOxal, and ACa could be used in 10, 8, and 7 cycles, respectively, keeping 40 % of its initial activity.
authors.affiliation: Departamento de Ciências Agrárias, Universidade Regional do Alto Uruguai e das Missões, URI Erechim, 743, 99709-910, Erechim, RS, Brazil. cindyelena506@hotmail.com.
authors.forename: Cindy Elena
authors.initials: CE
authors.lastname: Bustamante-Vargas
authors.affiliation: Departamento de Engenharia Química e de Alimentos, Universidade Federal de Santa Catarina UFSC, PO Box 476, 88040-900, Florianópolis, SC, Brazil.
authors.forename: Débora
authors.initials: D
authors.lastname: de Oliveira
authors.affiliation: Departamento de Ciências Agrárias, Universidade Regional do Alto Uruguai e das Missões, URI Erechim, 743, 99709-910, Erechim, RS, Brazil.
authors.forename: Eunice
authors.initials: E
authors.lastname: Valduga
authors.affiliation: Departamento de Ciências Agrárias, Universidade Regional do Alto Uruguai e das Missões, URI Erechim, 743, 99709-910, Erechim, RS, Brazil.
authors.forename: Luciana Dornelles
authors.initials: LD
authors.lastname: Venquiaruto
authors.affiliation: Departamento de Ciências Agrárias, Universidade Regional do Alto Uruguai e das Missões, URI Erechim, 743, 99709-910, Erechim, RS, Brazil.
authors.forename: Natalia
authors.initials: N
authors.lastname: Paroul
authors.affiliation: Departamento de Ciências Agrárias, Universidade Regional do Alto Uruguai e das Missões, URI Erechim, 743, 99709-910, Erechim, RS, Brazil.
authors.forename: Geciane Toniazzo
authors.initials: GT
authors.lastname: Backes
authors.affiliation: Departamento de Ciências Agrárias, Universidade Regional do Alto Uruguai e das Missões, URI Erechim, 743, 99709-910, Erechim, RS, Brazil. dallago@uricer.edu.br.
authors.forename: Rogério Marcos
authors.initials: RM
authors.lastname: Dallago
mesh_terms: D000464:Alginates / Q000737:chemistry*; D001234:Aspergillus niger / Q000201:enzymology; D032701:Biomimetics; D002129:Calcium Oxalate / Q000737:chemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007700:Kinetics; D011096:Polygalacturonase / Q000737:chemistry*; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D000464:Alginates; D004800:Enzymes, Immobilized; D006603:Hexuronic Acids; D014867:Water; D002129:Calcium Oxalate; D020723:Glucuronic Acid; D005780:Gelatin; D011096:Polygalacturonase
keywords: Biomimetic matrix; Calcium oxalate; Encapsulation; Immobilization; Pectinase