title: A T-RFLP database for the rapid profiling of methanogenic communities in anaerobic digesters.
abstract: We present a simple protocol for the cost- and time-efficient profiling of methanogens based on T-RFLP fingerprinting of mcrA amplicons. Sequence data were compiled from mesophilic lab-scale and full-scale biogas reactors operated under various conditions and fed with various substrates. The database facilitates the rapid identification of methanogens, thus reducing the need of cloning and sequencing.
authors.affiliation: UFZ - Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Permoserstr. 15, 04318 Leipzig, Germany.
authors.forename: Franziska
authors.initials: F
authors.lastname: Bühligen
authors.affiliation: UFZ - Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Permoserstr. 15, 04318 Leipzig, Germany.
authors.forename: Rico
authors.initials: R
authors.lastname: Lucas
authors.affiliation: UFZ - Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Permoserstr. 15, 04318 Leipzig, Germany.
authors.forename: Marcell
authors.initials: M
authors.lastname: Nikolausz
authors.affiliation: UFZ - Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Permoserstr. 15, 04318 Leipzig, Germany. Electronic address: sabine.kleinsteuber@ufz.de.
authors.forename: Sabine
authors.initials: S
authors.lastname: Kleinsteuber
mesh_terms: D000693:Anaerobiosis / Q000235:genetics*; D001105:Archaea / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D056804:Biofuels / Q000032:analysis; D019149:Bioreactors; D016172:DNA Fingerprinting; D004262:DNA Restriction Enzymes / Q000235:genetics / Q000378:metabolism; D030541:Databases, Genetic; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics / Q000378:metabolism; D008697:Methane / Q000096:biosynthesis*; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D056804:Biofuels; D004262:DNA Restriction Enzymes; C060629:endodeoxyribonuclease McrA; C060413:endodeoxyribonuclease MwoI; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; D008697:Methane
keywords: Biogas; BstNI; Community fingerprinting; Methanogenic archaea; MwoI; mcrA