title: Dose controlled low energy electron irradiator for biomolecular films.
abstract: We have developed a multi target, Low Energy Electron (LEE), precise dose controlled irradiator for biomolecular films. Up to seven samples can be irradiated one after another at any preset electron energy and dose under UHV conditions without venting the chamber. In addition, one more sample goes through all the steps except irradiation, which can be used as control for comparison with the irradiated samples. All the samples are protected against stray electron irradiation by biasing them at -20 V during the entire period, except during irradiation. Ethernet based communication electronics hardware, LEE beam control electronics and computer interface were developed in house. The user Graphical User Interface to control the irradiation and dose measurement was developed using National Instruments Lab Windows CVI. The working and reliability of the dose controlled irradiator has been fully tested over the electron energy range of 0.5 to 500 eV by studying LEE induced single strand breaks to ΦX174 RF1 dsDNA.
authors.affiliation: Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400 005, India.
authors.forename: S V K
authors.initials: SV
authors.lastname: Kumar
authors.affiliation: Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400 005, India.
authors.forename: Satej T
authors.initials: ST
authors.lastname: Tare
authors.affiliation: Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400 005, India.
authors.forename: Yogesh V
authors.initials: YV
authors.lastname: Upalekar
authors.affiliation: Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400 005, India.
authors.forename: Thupten
authors.initials: T
authors.lastname: Tsering
mesh_terms: D004247:DNA / Q000235:genetics; D053960:DNA Breaks / Q000528:radiation effects; D004583:Electrons; D004867:Equipment Design; D011829:Radiation Dosage; D055618:Radiation Equipment and Supplies; D011835:Radiation Protection; D012984:Software; D014584:User-Computer Interface
chemical_list: D004247:DNA