title: A proposal of "core enzyme" bioindicator in long-term Pb-Zn ore pollution areas based on topsoil property analysis.
abstract: To study the effects of long-term mining activities on the agricultural soil quality of Mengnuo town in Yunnan province, China, the heavy metal and soil enzyme activities of soil samples from 47 sites were examined. The results showed that long-term mining processes led to point source heavy metal pollution and Pb, Cd, Zn and As were the primary metal pollutants. Polyphenoloxidase was found the most sensitive soil enzyme activity and significantly correlated with almost all the metals (P < 0.05). Amylase (for C cycling), acid phosphatase (for P cycling) and catalase (for redox reaction) activities showed significantly positive correlations (P < 0.05) with Pb, Cd, Zn and As contents. The correlations between soil enzymes activities and Cd, Pb and Zn contents were verified in microcosm experiments, it was found that catalase activity had significant correlations (P < 0.05) with these three metals in short-term experiments using different soils under different conditions. Based on both field investigation and microcosm simulation analysis, oxidoreductases activities (rather than a specific enzyme activity) were suggested to be used as "core enzyme", which could simply and universally indicate the heavy metal pollution degrees of different environments. And hydrolases (for C, N, P and S recycling) could be used as a supplement to improve correlation accuracy for heavy metal indication in various polluted environments.
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. Electronic address: yangjsh1999@163.com.
authors.forename: JinShui
authors.initials: J
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. Electronic address: yangfenglong110@126.com.
authors.forename: FengLong
authors.initials: F
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. Electronic address: 593482212@qq.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. Electronic address: guanlan868@163.com.
authors.forename: GuanLan
authors.initials: G
authors.lastname: Xing
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. Electronic address: chunping_d@163.com.
authors.forename: ChunPing
authors.initials: C
authors.lastname: Deng
authors.affiliation: National Research Center of Geoanalysis, Beijing 100037, China. Electronic address: shenyating@cags.ac.cn.
authors.forename: YaTing
authors.initials: Y
authors.lastname: Shen
authors.affiliation: National Research Center of Geoanalysis, Beijing 100037, China. Electronic address: luoliqiang@cags.ac.cn.
authors.forename: LiQiang
authors.initials: L
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. Electronic address: bzhli@cau.edu.cn.
authors.forename: BaoZhen
authors.initials: B
authors.lastname: Li
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. Electronic address: hlyuan@cau.edu.cn.
authors.forename: HongLi
authors.initials: H
authors.lastname: Yuan
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000383:Agriculture; D000681:Amylases / Q000032:analysis; D002374:Catalase / Q000032:analysis; D004156:Catechol Oxidase / Q000032:analysis; D002681:China; D004787:Environmental Pollution; D007854:Lead / Q000032:analysis*; D008906:Mining; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis*; D015032:Zinc / Q000032:analysis*
chemical_list: D012987:Soil; D012989:Soil Pollutants; D007854:Lead; D004156:Catechol Oxidase; D002374:Catalase; D000135:Acid Phosphatase; D000681:Amylases; D015032:Zinc
keywords: Bioindicator; Heavy metal pollution; Oxidoreductase; Pb-Zn ore; Soil enzymes activities