title: Salivary agglutinin is the major component in human saliva that modulates the lectin pathway of the complement system.
abstract: Saliva interacts with blood after mucosal damage or leakage of gingival crevicular fluid. Surface-adsorbed salivary agglutinin (SAG) activates the lectin pathway (LP) of the complement system via mannose-binding lectin, while SAG in solution inhibits complement activation. In the present study we investigated if, next to SAG, whole and glandular saliva itself and other salivary glycoproteins activate or inhibit the LP. Complement activation was measured by detecting C4 deposition on microtiter plates coated with saliva or purified proteins. Complement inhibition was measured after incubating serum with saliva or proteins in microtiter plates coated with mannan, an LP activator. Adsorbed whole, sublingual and submandibular saliva showed LP-dependent complement activation. Blood group secretors, but not non-secretors, activated the LP. Saliva of both secretors and non-secretors inhibited C4 deposition on mannan. After depletion of SAG, saliva no longer inhibited the LP. Other salivary proteins, including amylase, MUC5B and histatin 2, did not activate or inhibit the LP. Surface-adsorbed whole saliva and glandular saliva samples activate the LP of complement, depending on the presence of SAG and the secretor status of the donor. In solution, saliva inhibits the LP, depending on the presence of SAG, but independent of the secretor status.
authors.affiliation: Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), VU University and University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Sabrina Tg
authors.initials: ST
authors.lastname: Gunput
authors.affiliation: Department of Immunopathology, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Diana
authors.initials: D
authors.lastname: Wouters
authors.affiliation: Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), VU University and University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Kamran
authors.initials: K
authors.lastname: Nazmi
authors.affiliation: Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), VU University and University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Nivedita
authors.initials: N
authors.lastname: Cukkemane
authors.affiliation: Department of Immunopathology, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Mieke
authors.initials: M
authors.lastname: Brouwer
authors.affiliation: Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), VU University and University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Enno Ci
authors.initials: EC
authors.lastname: Veerman
authors.affiliation: Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), VU University and University of Amsterdam, Amsterdam, The Netherlands a.ligtenberg@acta.nl.
authors.forename: Antoon Jm
authors.initials: AJ
authors.lastname: Ligtenberg
mesh_terms: D000327:Adsorption; D000328:Adult; D000681:Amylases / Q000378:metabolism; D001789:Blood Group Antigens; D002135:Calcium-Binding Proteins; D003181:Complement C4 / Q000378:metabolism; D037582:Complement Pathway, Mannose-Binding Lectin; D004268:DNA-Binding Proteins; D006801:Humans; D018928:Immunity, Mucosal; D008351:Mannans / Q000378:metabolism; D037601:Mannose-Binding Lectin / Q000172:deficiency* / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D008661:Metabolism, Inborn Errors / Q000235:genetics / Q000276:immunology*; D008875:Middle Aged; D055257:Mucin-5B / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D012463:Saliva / Q000276:immunology / Q000378:metabolism*; D025521:Tumor Suppressor Proteins
chemical_list: D001789:Blood Group Antigens; D002135:Calcium-Binding Proteins; D003181:Complement C4; C108174:DMBT1 protein, human; D004268:DNA-Binding Proteins; C524578:MUC5B protein, human; D008351:Mannans; D037601:Mannose-Binding Lectin; D055257:Mucin-5B; D011956:Receptors, Cell Surface; D025521:Tumor Suppressor Proteins; D000681:Amylases
keywords: Complement; mannose-binding lectin; saliva; salivary agglutinin; secretor status