title: Synergistic influence of topomimetic and chondroitin sulfate-based treatments on osteogenic potential of Ti-6Al-4V.
abstract: We combined topographical and chemical surface modifications of Ti-6Al-4V (TA6V) to improve its osteogenic potential. By acid-etching, we first generated topomimetic surface features resembling, in size and roughness, bone cavities left by osteoclasts. Next, we coated these surfaces with biomimetic Layer-by-Layer films (LbL), composed of chondroitin sulfate A and poly-l-lysine that were mechanically tuned after a post-treatment with genipin. The structural impact of each surface processing step was thoroughly inspected. The desired nano/microrough topographies of TA6V were maintained upon LbL deposition. Whereas no significant promotion of adhesion and proliferation of MC3T3-E1 preosteoblasts were detected after independent or combined modifications of the topography and the chemical composition of the substrates, osteogenic maturation was promoted when both surface treatments were combined, as was evidenced by significant long-term matrix mineralization. The results open promising route toward improved osseointegration of titanium-based implants. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1988-2000, 2016.
authors.affiliation: Normandie Université, Caen, France.|Laboratoire De Biophysique Et Biomatériaux (La2B - MERCI EA 3829), Université De Rouen, Centre Universitaire D'Évreux, 1 Rue Du 7ème Chasseurs, Évreux Cedex, 27002, France.
authors.forename: Béatrice
authors.initials: B
authors.lastname: Labat
authors.affiliation: Normandie Université, Caen, France.|Laboratoire De Biophysique Et Biomatériaux (La2B - MERCI EA 3829), Université De Rouen, Centre Universitaire D'Évreux, 1 Rue Du 7ème Chasseurs, Évreux Cedex, 27002, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Morin-Grognet
authors.affiliation: Normandie Université, Caen, France.|Laboratoire De Biophysique Et Biomatériaux (La2B - MERCI EA 3829), Université De Rouen, Centre Universitaire D'Évreux, 1 Rue Du 7ème Chasseurs, Évreux Cedex, 27002, France.
authors.forename: Fabien
authors.initials: F
authors.lastname: Gaudière
authors.affiliation: Normandie Université, Caen, France.|Laboratoire De Biophysique Et Biomatériaux (La2B - MERCI EA 3829), Université De Rouen, Centre Universitaire D'Évreux, 1 Rue Du 7ème Chasseurs, Évreux Cedex, 27002, France.
authors.forename: Lucia
authors.initials: L
authors.lastname: Bertolini-Forno
authors.affiliation: Normandie Université, Caen, France.|Laboratoire De Biophysique Et Biomatériaux (La2B - MERCI EA 3829), Université De Rouen, Centre Universitaire D'Évreux, 1 Rue Du 7ème Chasseurs, Évreux Cedex, 27002, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Thoumire
authors.affiliation: Normandie Université, Caen, France.|Laboratoire De Biophysique Et Biomatériaux (La2B - MERCI EA 3829), Université De Rouen, Centre Universitaire D'Évreux, 1 Rue Du 7ème Chasseurs, Évreux Cedex, 27002, France.|MERCI, EA 3829, Faculté De Médecine-Pharmacie, Université De Rouen, 22 Boulevard Gambetta, Rouen, 76183, France.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Vannier
authors.affiliation: Normandie Université, Caen, France.|Laboratoire De Biophysique Et Biomatériaux (La2B - MERCI EA 3829), Université De Rouen, Centre Universitaire D'Évreux, 1 Rue Du 7ème Chasseurs, Évreux Cedex, 27002, France.
authors.forename: Guy
authors.initials: G
authors.lastname: Ladam
authors.affiliation: Normandie Université, Caen, France.|Laboratoire De Biophysique Et Biomatériaux (La2B - MERCI EA 3829), Université De Rouen, Centre Universitaire D'Évreux, 1 Rue Du 7ème Chasseurs, Évreux Cedex, 27002, France.
authors.forename: Hassan
authors.initials: H
authors.lastname: Atmani
mesh_terms: D000497:Alloys; D000818:Animals; D040761:Biomimetic Materials / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002809:Chondroitin Sulfates / Q000494:pharmacology*; D051379:Mice; D008856:Microscopy, Fluorescence; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000497:Alloys; C031462:titanium alloy (TiAl6V4); D002809:Chondroitin Sulfates; D014025:Titanium
keywords: chondroitin sulfate; genipin; pre-osteoblasts; titanium alloy; topography