title: The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells.
abstract: Here, we developed a cell defined siRNA microarray (CDSM) for human bone marrow stromal cells (hBMSCs) designed to control the culture of cells inside the spot area without reducing the efficiency of siRNA silencing, "Development of a cell-defined siRNA microarray for analysis of gene functionin human bone marrow stromal cells" (Kim et al., 2016 [1]). First, we confirmed that p65 protein inhibition efficiency was maintained when hBMSCs were culture for 7 days on the siRNA spot, and siRNA spot activity remained in spite of long term storage (10 days and 2 months). Additionally, we confirmed p65 protein inhibition in U2OS cells after 48 h reverse transfection.
authors.affiliation: Institut Pasteur Korea, IP-Korea, 696 Sampyeong-dong, Bundang-gu, Seongnam-si, Gyeonggi-do 463-400, Republic of Korea; Department of Animal Biotechnology (BK21), Animal Resources Research Center, Konkuk University, Seoul 143-702, Republic of Korea.
authors.forename: Hi Chul
authors.initials: HC
authors.lastname: Kim
authors.affiliation: Biomedical Research Institute & IRICT, Seoul National University Hospital, Seoul, Republic of Korea.
authors.forename: Gi-Hwan
authors.initials: GH
authors.lastname: Kim
authors.affiliation: Institut Pasteur Korea, IP-Korea, 696 Sampyeong-dong, Bundang-gu, Seongnam-si, Gyeonggi-do 463-400, Republic of Korea.
authors.forename: David
authors.initials: D
authors.lastname: Shum
authors.affiliation: Department of Animal Biotechnology (BK21), Animal Resources Research Center, Konkuk University, Seoul 143-702, Republic of Korea.
authors.forename: Ssang-Goo
authors.initials: SG
authors.lastname: Cho
authors.affiliation: Biomedical Research Institute & IRICT, Seoul National University Hospital, Seoul, Republic of Korea.
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Lee
authors.affiliation: Institut Pasteur Korea, IP-Korea, 696 Sampyeong-dong, Bundang-gu, Seongnam-si, Gyeonggi-do 463-400, Republic of Korea.
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Kwon