title: Determining Maximum Glycolytic Capacity Using Extracellular Flux Measurements.
abstract: Measurements of glycolytic rate and maximum glycolytic capacity using extracellular flux analysis can give crucial information about cell status and phenotype during normal operation, development of pathology, differentiation, and malignant transformation. They are also of great use when assessing the effects of chemical or drug treatments. Here, we experimentally define maximum glycolytic capacity, demonstrate how it differs from glycolytic rate, and provide a protocol for determining the basal glycolytic rate and maximum glycolytic capacity in cells using extracellular flux measurements. The results illustrate the power of extracellular flux analysis to describe the energetics of adherent cells in culture in a fully quantitative way.
authors.affiliation: Touro University California College of Pharmacy, 1310 Club Drive, Vallejo, California, 94592, United States of America.|Buck Institute for Research on Aging, 8001 Redwood Blvd, Novato, California, 94945, United States of America.
authors.forename: Shona A
authors.initials: SA
authors.lastname: Mookerjee
authors.affiliation: Buck Institute for Research on Aging, 8001 Redwood Blvd, Novato, California, 94945, United States of America.
authors.forename: David G
authors.initials: DG
authors.lastname: Nicholls
authors.affiliation: Touro University California College of Pharmacy, 1310 Club Drive, Vallejo, California, 94592, United States of America.|Buck Institute for Research on Aging, 8001 Redwood Blvd, Novato, California, 94945, United States of America.
authors.forename: Martin D
authors.initials: MD
authors.lastname: Brand
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001671:Biochemistry / Q000379:methods; D002460:Cell Line; D019069:Cell Respiration; D005347:Fibroblasts / Q000378:metabolism*; D006019:Glycolysis; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D032446:Myoblasts / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
chemical_list: D000255:Adenosine Triphosphate; D000254:Sodium-Potassium-Exchanging ATPase