title: [Downregulation of Human Adenovirus DNA Polymerase Gene by Modified siRNAs].
abstract: Human adenoviruses, in particular D8, D19, and D37, cause ocular infections. Currently, there is no available causally directed treatment, which efficiently counteracts adenoviral infectious diseases. In our previous work, we showed that gene silencing by means of RNA interference is an effective approach for downregulation of human species D adenoviruses replication. In this study, we compared the biological activity of siRNAs and their modified analogs targeting human species D adenoviruses DNA polymerase. We found that one of selectively 2'-O-methyl modified siRNAs mediates stable and long-lasting suppression of the target gene (12 days post transfection). We suppose that this siRNA can be used as a potential therapeutic agent against human species D adenoviruses.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.
authors.forename: N A
authors.initials: NA
authors.lastname: Nikitenko
authors.affiliation: Heinrich Pette Institute-Leibniz Institute for Experimental Virology, Hamburg, D-20251 Germany.
authors.forename: T
authors.initials: T
authors.lastname: Speiseder
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia.
authors.forename: E L
authors.initials: EL
authors.lastname: Chernolovskaya
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia.
authors.forename: M A
authors.initials: MA
authors.lastname: Zenkova
authors.affiliation: Heinrich Pette Institute-Leibniz Institute for Experimental Virology, Hamburg, D-20251 Germany.
authors.forename: T
authors.initials: T
authors.lastname: Dobner
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.|prassolov45@mail.ru.
authors.forename: V S
authors.initials: VS
authors.lastname: Prassolov
mesh_terms: D000258:Adenovirus Infections, Human / Q000188:drug therapy / Q000821:virology; D000260:Adenoviruses, Human / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000096:biosynthesis / Q000235:genetics*; D015536:Down-Regulation / Q000187:drug effects*; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000494:pharmacology* / Q000627:therapeutic use; D000067492:RNAi Therapeutics; D014162:Transfection
chemical_list: D034741:RNA, Small Interfering; D004259:DNA-Directed DNA Polymerase
keywords: RNA interference; chemical modification of small interfering RNA; human adenoviruses