title: Amniotic fluid rapid biomarkers are associated with intra-amniotic infection in preterm pregnancies regardless of the membrane status.
abstract: The objective of this study was to evaluate the association of amniotic fluid lactate dehydrogenase and glucose concentrations with microbial invasion of amniotic cavity and histologic chorioamnionitis before 37 pregnancy weeks in women with or without preterm premature rupture of membranes.
  
  
  
  Amniocentesis was performed on 70 women with suspected intra-amniotic infection. Standard biochemical methods, molecular microbiology and culture techniques were used. Histopathological examination of the placenta was performed.
  
  
  
  Thirty (48%) women had microbial invasion of the amniotic cavity (MIAC), 53 (76%) women had histological chorioamnionitis and 28 women had both. The cutoff values for MIAC and histological chorioamnionitis were 429 IU l(-1) for lactate dehydrogenase and 0.7 mmol l(-1) for glucose. Both end points occurred equally often regardless of the membrane status.
  
  
  
  Increased amniotic fluid lactate dehydrogenase and decreased glucose were associated with MIAC and histological chorioamnionitis. However, test performance was of limited value.
authors.affiliation: Department of Obstetrics and Gynecology, Helsinki University Hospital and University of Helsinki, Helsinki, Finland.
authors.forename: T
authors.initials: T
authors.lastname: Myntti
authors.affiliation: Department of Obstetrics and Gynecology, Helsinki University Hospital and University of Helsinki, Helsinki, Finland.
authors.forename: L
authors.initials: L
authors.lastname: Rahkonen
authors.affiliation: Department of Obstetrics and Gynecology, Helsinki University Hospital and University of Helsinki, Helsinki, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Tikkanen
authors.affiliation: Division of Clinical Microbiology, HUSLAB, Helsinki University Hospital, Helsinki, Finland.
authors.forename: A
authors.initials: A
authors.lastname: Pätäri-Sampo
authors.affiliation: Department of Obstetrics and Gynecology, Helsinki University Hospital and University of Helsinki, Helsinki, Finland.
authors.forename: J
authors.initials: J
authors.lastname: Paavonen
authors.affiliation: Department of Obstetrics and Gynecology, Helsinki University Hospital and University of Helsinki, Helsinki, Finland.
authors.forename: V
authors.initials: V
authors.lastname: Stefanovic
mesh_terms: D000328:Adult; D000649:Amniocentesis; D000653:Amniotic Fluid / Q000737:chemistry / Q000382:microbiology*; D015415:Biomarkers / Q000032:analysis; D002821:Chorioamnionitis / Q000453:epidemiology* / Q000382:microbiology / Q000473:pathology; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000453:epidemiology*; D005387:Finland; D005865:Gestational Age; D005947:Glucose / Q000032:analysis*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D010920:Placenta / Q000473:pathology; D011247:Pregnancy; D011446:Prospective Studies; D012372:ROC Curve
chemical_list: D015415:Biomarkers; D007770:L-Lactate Dehydrogenase; D005947:Glucose