title: Corynebacterium lowii sp. nov. and Corynebacterium oculi sp. nov., derived from human clinical disease and an emended description of Corynebacterium mastitidis.
abstract: Strains of members of the genus Corynebacterium derived from ophthalmologic patients in Japan, Belgium and Switzerland and found to be closely related to-, but distinguishable from Corynebacterium mastitidis by 16S rRNA gene sequencing, were characterized using biochemical, chemotaxonomic, MALDI-TOF mass spectrometry and antimicrobial susceptibility methods and DNA-DNA hybridization as well as by whole-genome sequencing (WGS). Based on this investigation, we describe Corynebacterium lowii sp. nov. and Corynebacterium oculi sp. nov., derived from human ocular specimens, as well as emend the description of Corynebacterium mastitidis. Type strains for these species are: C. lowii R-50085T (=LMG 28276T =CCUG 65815T) and C. oculi R-50187T (=LMG 28277T =CCUG 65816T). DNA G+C content was found to be 62.2 % (by HPLC) and 62.8 % (by WGS) for C. lowii R-50085T, 64.1 % (HPLC) and 64.8 % (WGS) for C. oculi R-50187T and 67.8 % (HPLC) for C. mastitidis LMG 19040T [=S-8T =CCUG 38654T =CECT 4843T =CIP 105509T =DSM 44356T =IFO (NBRC)16160T =JCM 12269T].
authors.affiliation: Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada.|National Microbiology Laboratory-CSCHAH, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors.forename: Kathryn A
authors.initials: KA
authors.lastname: Bernard
authors.affiliation: National Microbiology Laboratory-CSCHAH, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors.forename: Ana Luisa
authors.initials: AL
authors.lastname: Pacheco
authors.affiliation: National Microbiology Laboratory-CSCHAH, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors.forename: Courtney
authors.initials: C
authors.lastname: Loomer
authors.affiliation: National Microbiology Laboratory-CSCHAH, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors.forename: Tamara
authors.initials: T
authors.lastname: Burdz
authors.affiliation: National Microbiology Laboratory-CSCHAH, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors.forename: Deborah
authors.initials: D
authors.lastname: Wiebe
authors.affiliation: National Microbiology Laboratory-CSCHAH, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors.forename: Chris
authors.initials: C
authors.lastname: Huynh
authors.affiliation: National Microbiology Laboratory-CSCHAH, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors.forename: Brynn
authors.initials: B
authors.lastname: Kaplen
authors.affiliation: National Microbiology Laboratory-CSCHAH, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors.forename: Adam B
authors.initials: AB
authors.lastname: Olson
authors.affiliation: Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium.
authors.forename: Margo
authors.initials: M
authors.lastname: Cnockaert
authors.affiliation: Department of Ophthalmology, Sakai Hospital, Kinki University Faculty of Medicine, Osaka, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Eguchi
authors.affiliation: Department of Microbiology, Faculty of Medicine, Kagawa University, Kagawa, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Kuwahara
authors.affiliation: Department of Microbiology, Faculty of Medicine, Kagawa University, Kagawa, Japan.
authors.forename: Haruyuki
authors.initials: H
authors.lastname: Nakayama-Imaohji
authors.affiliation: Department of Ophthalmology, Kaisei General Hospital, Kagawa, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shiota
authors.affiliation: Department of Microbiology, AZ Groeninge Hospital, Kortrijk, Belgium.
authors.forename: Michael
authors.initials: M
authors.lastname: Boudewijns
authors.affiliation: Department of Microbiology, Sint Andries Hospital of Tielt, Tielt, Belgium.
authors.forename: Frederik
authors.initials: F
authors.lastname: Van Hoecke
authors.affiliation: Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium.
authors.forename: Peter
authors.initials: P
authors.lastname: Vandamme
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D001530:Belgium; D003352:Corynebacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005123:Eye / Q000382:microbiology*; D005227:Fatty Acids / Q000737:chemistry; D006801:Humans; D007564:Japan; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013557:Switzerland
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S