title: MicroRNA profiling of cisplatin-resistant oral squamous cell carcinoma cell lines enriched with cancer-stem-cell-like and epithelial-mesenchymal transition-type features.
abstract: Oral cancer is of major public health problem in India. Current investigation was aimed to identify the specific deregulated miRNAs which are responsible for development of resistance phenotype through regulating their resistance related target gene expression in oral squamous cell carcinoma (OSCC). Cisplatin-resistant OSCC cell lines were developed from their parental human OSCC cell lines and subsequently characterised. The resistant cells exhibited enhanced proliferative, clonogenic capacity with significant up-regulation of P-glycoprotein (ABCB1), c-Myc, survivin, β-catenin and a putative cancer-stem-like signature with increased expression of CD44, whereas the loss of E-cadherin signifies induced EMT phenotype. A comparative analysis of miRNA expression profiling in parental and cisplatin-resistant OSCC cell lines for a selected sets (deregulated miRNAs in head and neck cancer) revealed resistance specific signature. Moreover, we observed similar expression pattern for these resistance specific signature miRNAs in neoadjuvant chemotherapy treated and recurrent tumours compared to those with newly diagnosed primary tumours in patients with OSCC. All these results revealed that these miRNAs play an important role in the development of cisplatin-resistance mainly through modulating cancer stem-cell-like and EMT-type properties in OSCC.
authors.affiliation: Cancer Biology and Inflammatory Disorder Division, CSIR-Indian Institute of Chemical Biology, Kolkata, India.
authors.forename: Ruma Dey
authors.initials: RD
authors.lastname: Ghosh
authors.affiliation: Cancer Biology and Inflammatory Disorder Division, CSIR-Indian Institute of Chemical Biology, Kolkata, India.
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Ghuwalewala
authors.affiliation: Cancer Biology and Inflammatory Disorder Division, CSIR-Indian Institute of Chemical Biology, Kolkata, India.
authors.forename: Pijush
authors.initials: P
authors.lastname: Das
authors.affiliation: Structural Biology and Bio-Informatics Division, CSIR-Indian Institute of Chemical Biology, Kolkata, India.
authors.forename: Sapan
authors.initials: S
authors.lastname: Mandloi
authors.affiliation: Cancer Biology and Inflammatory Disorder Division, CSIR-Indian Institute of Chemical Biology, Kolkata, India.
authors.forename: Sk Kayum
authors.initials: SK
authors.lastname: Alam
authors.affiliation: Department of Surgical Oncology, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, Kolkata, India.
authors.forename: Jayanta
authors.initials: J
authors.lastname: Chakraborty
authors.affiliation: Department of Surgical Oncology, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, Kolkata, India.
authors.forename: Sajal
authors.initials: S
authors.lastname: Sarkar
authors.affiliation: Structural Biology and Bio-Informatics Division, CSIR-Indian Institute of Chemical Biology, Kolkata, India.
authors.forename: Saikat
authors.initials: S
authors.lastname: Chakrabarti
authors.affiliation: Department of Oncogene Regulation, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, Kolkata, India.
authors.forename: Chinmoy Kumar
authors.initials: CK
authors.lastname: Panda
authors.affiliation: Cancer Biology and Inflammatory Disorder Division, CSIR-Indian Institute of Chemical Biology, Kolkata, India.
authors.forename: Susanta
authors.initials: S
authors.lastname: Roychoudhury
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000378:metabolism; D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D018929:Cell Culture Techniques; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement; D002945:Cisplatin / Q000737:chemistry*; D019008:Drug Resistance, Neoplasm; D058750:Epithelial-Mesenchymal Transition; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D007194:India; D020128:Inhibitory Concentration 50; D035683:MicroRNAs / Q000378:metabolism*; D009062:Mouth Neoplasms / Q000378:metabolism*; D020360:Neoadjuvant Therapy; D009364:Neoplasm Recurrence, Local / Q000235:genetics; D014411:Neoplastic Stem Cells / Q000378:metabolism; D010641:Phenotype; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; C497483:CD44 protein, human; C495270:CTNNB1 protein, human; D018960:Hyaluronan Receptors; D035683:MicroRNAs; D016271:Proto-Oncogene Proteins c-myc; D051176:beta Catenin; D002945:Cisplatin