title: Identification of candidate genes for an early-maturing soybean mutant by genome resequencing analysis.
abstract: Flowering is indicative of the transition from vegetative to reproductive phase, a critical event in the life cycle of plants. In this study, we performed whole genome resequencing by Illumina HiSeq to identify changes in flowering genes using an early-flowering phenotype of soybean mutant line Josaengserori (JS) derived from Korean landrace, Seoritae (SR), and we obtained mapped reads of 131,769,690 and 167,669,640 bp in JS and SR, respectively. From the whole genome sequencing results between JS and SR, we identified 332,821 polymorphic SNPs and 65,178 indels, respectively. Among these, 30 flowering genes were in SNPs and 25 were in indels. Among 30 flowering genes detected in SNPs, Glyma02g33040, Glyma06g22650, Glyma10g36600, Glyma13g01290, Glyma14g10530, Glyma16g01980, Glyma17g11040, Glyma18g53690, and Glyma20g29300 were non-synonymous substitutions between JS and SR. Changes in Glyma10g36600 (GI), Glya02g33040 (AGL18), Glyma17g11040 (TOC1), and Glyma14g10530 (ELF3) in JS affected the expression of GmFT2a and resulted in early flowering. These results provide insight into the regulatory pathways of flowering in soybean mutants and help to improve our knowledge of soybean mutation breeding.
authors.affiliation: Radiation Breeding Research Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, 580-185, Republic of Korea.|National Agrobiodiversity Center, NAAS, RDA, Jeonju, 560-500, Republic of Korea.
authors.forename: Kyung Jun
authors.initials: KJ
authors.lastname: Lee
authors.affiliation: Radiation Breeding Research Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, 580-185, Republic of Korea.
authors.forename: Dong Sub
authors.initials: DS
authors.lastname: Kim
authors.affiliation: Radiation Breeding Research Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, 580-185, Republic of Korea. jbkim74@kaeri.re.kr.
authors.forename: Jin-Baek
authors.initials: JB
authors.lastname: Kim
authors.affiliation: Seeders Inc., Daejeon, 305-509, Republic of Korea.
authors.forename: Sung-Hwan
authors.initials: SH
authors.lastname: Jo
authors.affiliation: Radiation Breeding Research Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, 580-185, Republic of Korea.
authors.forename: Si-Yong
authors.initials: SY
authors.lastname: Kang
authors.affiliation: Radiation Breeding Research Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, 580-185, Republic of Korea.
authors.forename: Hong-Il
authors.initials: HI
authors.lastname: Choi
authors.affiliation: Division of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, 500-757, Republic of Korea. bkha@jnu.ac.kr.
authors.forename: Bo-Keun
authors.initials: BK
authors.lastname: Ha
mesh_terms: D002874:Chromosome Mapping / Q000379:methods; D032461:Chromosomes, Plant / Q000235:genetics; D035264:Flowers / Q000235:genetics*; D015723:Gene Library; D053263:Gene Regulatory Networks; D018745:Genome, Plant; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D054643:INDEL Mutation; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D017422:Sequence Analysis, DNA / Q000379:methods*; D013025:Glycine max / Q000235:genetics / Q000502:physiology*
chemical_list: D010940:Plant Proteins
keywords: Early flowering; Gamma-ray; Mutation; Resequencing; Soybean