title: Postnatal development of the myenteric glial network and its modulation by butyrate.
abstract: The postnatal period is crucial for the development of gastrointestinal (GI) functions. The enteric nervous system is a key regulator of GI functions, and increasing evidences indicate that 1) postnatal maturation of enteric neurons affect the development of GI functions, and 2) microbiota-derived short-chain fatty acids can be involved in this maturation. Although enteric glial cells (EGC) are central regulators of GI functions, the postnatal evolution of their phenotype remains poorly defined. We thus characterized the postnatal evolution of EGC phenotype in the colon of rat pups and studied the effect of short-chain fatty acids on their maturation. We showed an increased expression of the glial markers GFAP and S100β during the first postnatal week. As demonstrated by immunohistochemistry, a structured myenteric glial network was observed at 36 days in the rat colons. Butyrate inhibited EGC proliferation in vivo and in vitro but had no effect on glial marker expression. These results indicate that the EGC myenteric network continues to develop after birth, and luminal factors such as butyrate endogenously produced in the colon may affect this development.
authors.affiliation: INSERM, U913, Nantes, France; Université Nantes, Nantes, France; CHU Nantes, Hôtel Dieu, Institut des Maladies de l'Appareil Digestif, France; and Centre de Recherche en Nutrition Humaine, Nantes, France.
authors.forename: François
authors.initials: F
authors.lastname: Cossais
authors.affiliation: INSERM, U913, Nantes, France; Université Nantes, Nantes, France; CHU Nantes, Hôtel Dieu, Institut des Maladies de l'Appareil Digestif, France; and Centre de Recherche en Nutrition Humaine, Nantes, France.
authors.forename: Tony
authors.initials: T
authors.lastname: Durand
authors.affiliation: INSERM, U913, Nantes, France; Université Nantes, Nantes, France; CHU Nantes, Hôtel Dieu, Institut des Maladies de l'Appareil Digestif, France; and Centre de Recherche en Nutrition Humaine, Nantes, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Chevalier
authors.affiliation: INSERM, U913, Nantes, France; Université Nantes, Nantes, France; CHU Nantes, Hôtel Dieu, Institut des Maladies de l'Appareil Digestif, France; and Centre de Recherche en Nutrition Humaine, Nantes, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Boudaud
authors.affiliation: INSERM, U913, Nantes, France; Université Nantes, Nantes, France; CHU Nantes, Hôtel Dieu, Institut des Maladies de l'Appareil Digestif, France; and Centre de Recherche en Nutrition Humaine, Nantes, France.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Kermarrec
authors.affiliation: INSERM, U913, Nantes, France; Université Nantes, Nantes, France; CHU Nantes, Hôtel Dieu, Institut des Maladies de l'Appareil Digestif, France; and Centre de Recherche en Nutrition Humaine, Nantes, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Aubert
authors.affiliation: INSERM, U913, Nantes, France; Université Nantes, Nantes, France; CHU Nantes, Hôtel Dieu, Institut des Maladies de l'Appareil Digestif, France; and Centre de Recherche en Nutrition Humaine, Nantes, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Neveu
authors.affiliation: INSERM, U913, Nantes, France; Université Nantes, Nantes, France; CHU Nantes, Hôtel Dieu, Institut des Maladies de l'Appareil Digestif, France; and Centre de Recherche en Nutrition Humaine, Nantes, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Naveilhan
authors.affiliation: INSERM, U913, Nantes, France; Université Nantes, Nantes, France; CHU Nantes, Hôtel Dieu, Institut des Maladies de l'Appareil Digestif, France; and Centre de Recherche en Nutrition Humaine, Nantes, France Michel.Neunlist@univ-nantes.fr.
authors.forename: Michel
authors.initials: M
authors.lastname: Neunlist
mesh_terms: D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D002460:Cell Line; D002478:Cells, Cultured; D003106:Colon / Q000166:cytology / Q000254:growth & development / Q000294:innervation / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics / Q000378:metabolism; D009197:Myenteric Plexus / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D055495:Neurogenesis; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010641:Phenotype; D051381:Rats; D017207:Rats, Sprague-Dawley; D009418:S100 Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D002087:Butyrates; D005227:Fatty Acids; C000614142:Gfap protein, rat; D005904:Glial Fibrillary Acidic Protein; D009418:S100 Proteins
keywords: HDAC; MCT; butyrate; enteric glial cells; maturation