title: Construction of a Sequencing Library from Circulating Cell-Free DNA.
abstract: Circulating DNA is cell-free DNA (cfDNA) in serum or plasma that can be used for non-invasive prenatal testing, as well as cancer diagnosis, prognosis, and stratification. High-throughput sequence analysis of the cfDNA with next-generation sequencing technologies has proven to be a highly sensitive and specific method in detecting and characterizing mutations in cancer and other diseases, as well as aneuploidy during pregnancy. This unit describes detailed procedures to extract circulating cfDNA from human serum and plasma and generate sequencing libraries from a wide concentration range of circulating DNA.
authors.affiliation: QIAGEN GmbH, Hilden, Germany.
authors.forename: Nan
authors.initials: N
authors.lastname: Fang
authors.affiliation: BIOMILLENIA, Paris, France.
authors.forename: Dirk
authors.initials: D
authors.lastname: Löffert
authors.affiliation: QIAGEN GmbH, Hilden, Germany.
authors.forename: Rumeysa
authors.initials: R
authors.lastname: Akinci-Tolun
authors.affiliation: QIAGEN GmbH, Hilden, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Heitz
authors.affiliation: QIAGEN GmbH, Hilden, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Wolf
mesh_terms: D004247:DNA / Q000097:blood / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D015723:Gene Library; D005820:Genetic Testing; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D011247:Pregnancy; D011296:Prenatal Diagnosis
chemical_list: D004247:DNA
keywords: NGS; cfDNA; circulating DNA; low input; sequencing library preparation