title: Pseudorhodobacter sinensis sp. nov. and Pseudorhodobacter aquaticus sp. nov., isolated from crater lakes.
abstract: Three Gram-stain negative, aerobic, non-motile, rod-shaped bacterial strains, Y1R2-4T, Y3R2-3 and DC2N1-10T, isolated from two crater lakes of the Daxinganling Mountains, northern China, were studied to determine their taxonomic position. They grew at 4-30 °C (optimally at 20-25 °C), at pH 6.0-7.5 (optimally at pH 7.0) and in the presence of 0-0.5 % (w/v) NaCl. On the basis of 16S rRNA gene sequence analysis, these strains showed 95.3-96.6 % similarity to members of the genus Pseudorhodobacter, including Pseudorhodobacter ferrugineus DSM 5888T, Pseudorhodobacter wandonensis WT-MW11T, Pseudorhodobacter antarcticus ZS3-33T and Pseudorhodobacter aquimaris HDW-19T. All strains contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The main polar lipids for strains Y1R2-4T and Y3R2-3 were phosphatidylglycerol, phosphatidylcholine, one unidentiﬁed aminophospholipid, one unidentiﬁed aminolipid, three unidentiﬁed phospholipids and two unidentiﬁed lipids, and those for strain DC2N1-10T were phosphatidylglycerol, phosphatidylcholine, one unidentiﬁed aminophospholipid, one unidentiﬁed aminolipid, one unidentiﬁed phospholipid and several unidentiﬁed lipids. The DNA G+C contents of strains Y1R2-4T, Y3R2-3 and DC2N1-10T were 61.9, 61.0 and 60.0 mol%, respectively. In addition, strain Y1R2-4T shared less than 50 % DNA-DNA relatedness to strain DC2N1-10T. Based on these differences in genetic, physiological and chemotaxonomic properties, strains Y1R2-4T, Y3R2-3 and DC2N1-10T were considered to represent two novel species of the genus Pseudorhodobacter, for which the names Pseudorhodobacter sinensis sp. nov. (type strain Y1R2-4T=CGMCC1.14435T=KCTC 52039T) and Pseudorhodobacter aquaticus sp. nov. (type strain DC2N1-10T=CGMCC1.14433T=KCTC 52040T) are proposed.
authors.affiliation: China General Microbiological Culture Collection Center and State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100101 Beijing, PR China.
authors.forename: Ai-Hua
authors.initials: AH
authors.lastname: Li
authors.affiliation: China General Microbiological Culture Collection Center and State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100101 Beijing, PR China.
authors.forename: Hong-Can
authors.initials: HC
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, 100101 Beijing, PR China.
authors.forename: Wei-Guo
authors.initials: WG
authors.lastname: Hou
authors.affiliation: China General Microbiological Culture Collection Center and State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100101 Beijing, PR China.
authors.forename: Yu-Guang
authors.initials: YG
authors.lastname: Zhou
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D060106:Lakes / Q000382:microbiology*; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C024989:coenzyme Q10