title: [TYPING OF VIBRIO CHOLERAE NON O1/NON O139 STRAINS, ISOLATED IN ROSTOV REGION IN 2014].
abstract: OBJECTIVE
  Comparative study of antibiotics resistance and VNTR-typing of Vibrio cholerae non O1/ non O139 strains, isolated on the territory of Rostov region in 2014.
  
  
  METHODS
  Antibioticogramms of strains were determined by serial dilution method in dense nutrient medium according to MG 4.2.2495-09 (2009). Pheno-, sero- and VNTR-typing was carried out by conventional-methods.
  
  
  RESULTS
  The studied strains belonged to V. cholerae species, did not agglutinate with O1 and O139 sera, were atoxigenic hemolysis-positive, did not contain genes of cholera toxin and toxin-coregulating pili of adhesion, contained genes of hemagglutinin/protease, protease PrtV, collagenase, cytotonic factor Cef, outer membrane protein-OmpW, tol- and -vps-clusters, regulatory genes toxR and hapR. Antibioticogramms of the strains have shown the presence of cultures, resistant to ampicillin, ceftazidime-furazolidone, trimethoprim/sulfamethoxazole with intermediate resistance to streptomycin, kanamycin, gentamycin, amikacin, netilmicin, Approximately 20% of isolates had multiple drug resistance. Data of VNTR- and genotyping confirmed a possibility of water transmission route of the infection.
  
  
  CONCLUSIONS
  Execution of monitoring of cultures from environmental samples is necessary for timely detection of genetic characteristics, antibiotics resistance.
authors.forename: N A
authors.initials: NA
authors.lastname: Selyanskaya
authors.forename: I V
authors.initials: IV
authors.lastname: Arkhangelskaya
authors.forename: A S
authors.initials: AS
authors.lastname: Vodopianov
authors.forename: S O
authors.initials: SO
authors.lastname: Vodopianov
authors.forename: V D
authors.initials: VD
authors.lastname: Kruglikov
authors.forename: S Yu
authors.initials: SY
authors.lastname: Vodyanitskaya
authors.forename: L M
authors.initials: LM
authors.lastname: Verkina
authors.forename: N B
authors.initials: NB
authors.lastname: Nepomnyaschaya
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002771:Cholera / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology / Q000635:transmission; D002772:Cholera Toxin / Q000235:genetics / Q000378:metabolism; D017364:Collagenases / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D024901:Drug Resistance, Multiple, Bacterial; D062665:Epidemiological Monitoring; D010861:Fimbriae, Bacterial; D017353:Gene Deletion; D005798:Genes, Bacterial; D006801:Humans; D007106:Immune Sera / Q000737:chemistry; D008666:Metalloendopeptidases / Q000235:genetics / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D010447:Peptide Hydrolases / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D012426:Russia / Q000453:epidemiology; D012703:Serotyping; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D041242:Vibrio cholerae O139 / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D044163:Vibrio cholerae non-O1 / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D014871:Water Microbiology
chemical_list: D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D007106:Immune Sera; D014157:Transcription Factors; C499324:toxR protein, Vibrio cholerae; D002772:Cholera Toxin; D010447:Peptide Hydrolases; C513132:PrtV protein, Vibrio cholerae; D017364:Collagenases; D008666:Metalloendopeptidases; C039814:hemagglutinin-protease