title: Effects of gonadotropin-releasing hormone agonist and antagonist on ovarian activity in a mouse model for polycystic ovary.
abstract: PCOS is a major cause of anovulatory infertility in women in their reproductive age. However, its etiology and pathophysiology remain uncertain. The immature mice chronically injected with DHEA, termed as PCO-mice, develop numerous large cystic follicles, high circulating androgen and anovulation similar to PCOS in women. Although PCO-mice show decreased ovarian GnRH I-receptor in immunoblot but show increased immunostaining for GnRH I-receptor in oocytes of cystic follicles. PCO-mice show reduced ovarian LH receptor expression, circulating estradiol and progesterone level compared to normal mice injected with vehicle only. The treatment with low dose of GnRH-Agonist in PCO-mice restores ovarian LH receptor expression to the level of normal mice and promote ovulation and formation of functional corpus luteum. GnRH-Antagonist although cause ovulation in PCO-mice but does not restore LH receptor expression to the level of normal mice, and they show low circulating progesterone and hypertrophied vacuolated corpus luteum. Our study suggests that GnRH-agonist restores ovulation in PCO-mice and produces biphasic and beneficial effect over the use of GnRH-Antagonist.
authors.affiliation: Department of Zoology, Banaras Hindu University, Varanasi-221001, India; Department of Zoology, Indira Gandhi National Tribal University, Amarkantak, MP, India. Electronic address: padmasanasingh@gmail.com.
authors.forename: Padmasana
authors.initials: P
authors.lastname: Singh
authors.affiliation: Department of Zoology, Banaras Hindu University, Varanasi-221001, India; Center for Life Sciences, National University of Singapore, Singapore-117456, Singapore. Electronic address: phsrks@nus.edu.sg.
authors.forename: Raj Kamal
authors.initials: RK
authors.lastname: Srivastava
authors.affiliation: Department of Zoology, Banaras Hindu University, Varanasi-221001, India. Electronic address: akrishna_ak@yahoo.co.in.
authors.forename: Amitabh
authors.initials: A
authors.lastname: Krishna
mesh_terms: D000818:Animals; D003338:Corpus Luteum / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D003687:Dehydroepiandrosterone / Q000008:administration & dosage; D004195:Disease Models, Animal; D004958:Estradiol / Q000097:blood; D005260:Female; D005786:Gene Expression Regulation; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D007986:Luteinizing Hormone / Q000235:genetics / Q000378:metabolism; D051379:Mice; D011085:Polycystic Ovary Syndrome / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D011374:Progesterone / Q000097:blood; D011966:Receptors, LHRH / Q000819:agonists / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism
chemical_list: D011966:Receptors, LHRH; D007987:Gonadotropin-Releasing Hormone; D003687:Dehydroepiandrosterone; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone
keywords: GnRH I; GnRH I-receptor; GnRH agonist; GnRH antagonist; PCOS