title: Molecular phylogenetic analysis of Rhizobium sullae isolated from Algerian Hedysarum flexuosum.
abstract: Isolates from root nodules of Hedysarum flexuosum, sampled from north region of Algeria, were analyzed on the basis of their phenotypic and molecular characteristics. They were tested for their tolerance to NaCl, pH, temperatures, antibiotics and heavy metals resistance. Interestingly, the isolate Hf_04N appeared resistant to ZnCl2 (50 μg/mL) and grew at high saline concentration up to 9 %. The phylogenetic positions of five isolates were studied by comparative sequence analysis of 16S rRNA, recA, nifH and nodD genes. There were grouped close to the Rhizobium sullae type strain in relation to their 16S rRNA, recA and nifH genes-based phylogenies. By contrast, the tree of nodD gene was not congruent with ribosomal, housekeeping and nitrogen fixation genes. We suggest that our strains have a novel nodD gene. The detection of conserved domains of NodD protein and nitrogenase reductase enzyme, confirm their ability to nodulate and fix nitrogen.
authors.affiliation: Laboratory of Genetics, Faculty of Sciences of Bizerte, 7021, Zarzouna, Tunisia. a_khadidja88@yahoo.fr.
authors.forename: Khadidja
authors.initials: K
authors.lastname: Aliliche
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Faculty of Sciences of Bizerte, Carthage University, 7021, Zarzouna, Tunisia.
authors.forename: Hamida
authors.initials: H
authors.lastname: Beghalem
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Faculty of Sciences of Bizerte, Carthage University, 7021, Zarzouna, Tunisia.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Landoulsi
authors.affiliation: Laboratory of Genetics, Faculty of Sciences of Bizerte, 7021, Zarzouna, Tunisia.
authors.forename: Ali
authors.initials: A
authors.lastname: Chriki
mesh_terms: D000462:Algeria; D001483:Base Sequence; D007887:Fabaceae / Q000382:microbiology*; D005798:Genes, Bacterial; D009586:Nitrogen Fixation; D010641:Phenotype; D010802:Phylogeny; D055170:Plant Root Nodulation; D016133:Polymerase Chain Reaction; D012231:Rhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D053204:Root Nodules, Plant / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
chemical_list: D012987:Soil
keywords: 16S rRNA; Hedysarum flexuosum; Rhizobium sullae; nifH; nodD; recA