title: Reproducible elimination of Clostridium difficile spores using a clinical area washer disinfector in 3 different health care sites.
abstract: Following a Clostridium difficile infection outbreak, the Infection Prevention and Control team at our institution queried the risk of transmission via bedpans reprocessed in washer disinfectors (WDs). This study's objective was to determine the effectiveness of the mechanical action, detergent, and temperature on the eradication of C difficile spores in 1 type of WD model.
  
  
  
  Three types of reusable bedpans/pots were inoculated with sterile human feces that contained 1 × 10(7) CFU/mL C difficile spores. The 0.3 mL fecal-spore suspension was inoculated in sealed cryovials. These items were reprocessed using the longest wash cycle of WDs in 9 clinical units, and then tested for residual C difficile spores. The number of colonies on each replicate organism detection and counting plate was recorded after anaerobic incubation at 35°C for 48 hours, and the log reduction was calculated.
  
  
  
  All 9 WDs met the manufacturer's operational specifications. Forty-three (96%) of 45 bedpans had no viable spores (>5.9 log10C difficile spore reduction). Two bedpans had 1 to 2 spores remaining. Viable C difficile spores were isolated from all 9 cryovials.
  
  
  
  Results demonstrated that when operating the WD as stipulated, C difficile spores were satisfactorily eliminated from bedpan surfaces. Temperature alone was insufficient to kill C difficile spores. It also suggested the importance of staff training, machine maintenance, and WD purchase specifications.
authors.affiliation: Infection Prevention and Control Program, Island Health, Victoria, British Columbia, Canada.
authors.forename: Kelly
authors.initials: K
authors.lastname: MacDonald
authors.affiliation: Department of Laboratory Medicine, Pathology, & Medical Genetics, Island Health, Victoria, British Columbia, Canada.
authors.forename: Jane
authors.initials: J
authors.lastname: Bishop
authors.affiliation: Infection Prevention and Control Program, Island Health, Victoria, British Columbia, Canada.
authors.forename: Bev
authors.initials: B
authors.lastname: Dobbyn
authors.affiliation: Infection Prevention and Control Program, Island Health, Victoria, British Columbia, Canada; Department of Laboratory Medicine, Pathology, & Medical Genetics, Island Health, Victoria, British Columbia, Canada. Electronic address: Pamela.kibsey@viha.ca.
authors.forename: Pamela
authors.initials: P
authors.lastname: Kibsey
authors.affiliation: Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada; St Boniface General Hospital Research Centre, Winnipeg, Manitoba, Canada.
authors.forename: Michelle J
authors.initials: MJ
authors.lastname: Alfa
mesh_terms: D000072296:Bathroom Equipment / Q000382:microbiology*; D016360:Clostridioides difficile / Q000302:isolation & purification*; D004203:Disinfection / Q000379:methods*; D006268:Health Facilities; D006801:Humans; D015203:Reproducibility of Results; D013170:Spores / Q000302:isolation & purification*; D013696:Temperature
keywords: Bedpan decontamination; Cleaning efficacy; Thermal conditions