title: Surgical margins in head and neck squamous cell carcinoma: Effect of heat artifact on immunohistochemistry as a future tool for assessment.
abstract: Margins in head and neck squamous cell carcinoma (HNSCC) are determined by morphological changes assessed via hematoxylin-eosin staining. Physiological changes may not be detected by this technique. The purpose of this study was to determine if a protein biomarker, laminin-332γ2, overexpressed in cancer cells at the invasive front in HNSCC, remains unaffected by heat produced during resection, supporting a role for immunohistochemistry assessment of margins.
  
  
  
  Archived tissue blocks from glottic squamous cell carcinomas (SCCs) resected by CO2 laser likely to contain both cancer cells and artifact were identified; 129-paired slides were obtained. One slide of each pair was stained with hematoxylin-eosin; the second stained for laminin-332γ2. The presence of cancer cells, artifact, and positive laminin-332γ2 staining was recorded. Twenty-seven pairs met the inclusion criteria.
  
  
  
  Immunohistochemistry staining of laminin-332γ is preserved in presence of heat artifact.
  
  
  
  This study supports use of immunohistochemistry to assess margins. © 2016 Wiley Periodicals, Inc. Head Neck 38: 1401-1406, 2016.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Monash Health (previously Southern Health), Melbourne, Victoria, Australia.|Department of Surgery (Monash Medical Centre), Monash University, Melbourne, Victoria, Australia.|Hudson Institute of Medical Research, Melbourne, Victoria, Australia.
authors.forename: Elizabeth A W
authors.initials: EA
authors.lastname: Sigston
authors.affiliation: Department of Anatomical Pathology, Monash Health (previously Southern Health), Melbourne, Victoria, Australia.
authors.forename: Anthony
authors.initials: A
authors.lastname: Longano
authors.affiliation: Hudson Institute of Medical Research, Melbourne, Victoria, Australia.|Department of Molecular and Translational Science, Monash University, Melbourne, Victoria, Australia.
authors.forename: Aneta T
authors.initials: AT
authors.lastname: Strzelecki
authors.affiliation: Hudson Institute of Medical Research, Melbourne, Victoria, Australia.|Department of Molecular and Translational Science, Monash University, Melbourne, Victoria, Australia.
authors.forename: Bryan R G
authors.initials: BR
authors.lastname: Williams
mesh_terms: D016477:Artifacts; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D015815:Cell Adhesion Molecules / Q000737:chemistry*; D005260:Female; D006258:Head and Neck Neoplasms / Q000473:pathology*; D006358:Hot Temperature; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008297:Male; D000072662:Margins of Excision; D012189:Retrospective Studies; D012307:Risk Factors; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D000077195:Squamous Cell Carcinoma of Head and Neck; D013194:Staining and Labeling / Q000379:methods; D000097794:Kalinin
chemical_list: D015815:Cell Adhesion Molecules; D000097794:Kalinin
keywords: CO2 laser; biomarkers; glottis squamous cell carcinoma; head and neck squamous cell carcinoma; laminin-332γ2; laser artifact; tissue artifact