title: [Study on the expression of T, B lymphocyte antigen and platelet antibodies in patients with platelet transfusion refractoriness].
abstract: OBJECTIVE
  To explore whether T lymphocytes subgroup, B lymphocytes, platelet antigen CD41a, CD61 or platelet antibodies are involved in the platelet transfusion refractoriness.
  
  
  METHODS
  Forty-seven patients diagnosed as platelet transfusion refractoriness and 32 patients that achieved effective platelet therapy were ennolled in this study. Flow cytometry was performed to detect the proportion of cytotoxic T cell (CD3(+)CD4(-)CD8(+)), helper T cell (CD3(+)CD4(+)CD8(-)) and B lymphocytes (CD19 (+) ), and the expression of platelet glycoproteins, including CD41a and CD61, while platelet antibodies were also measured by solid-phase agglutination.
  
  
  RESULTS
  The significant lower level of helper T cell (36.60% vs 48.53%), higher level of cytotoxic T cell (53.26% vs 44.02%) and lower cytotoxic/helper T cell ratio (0.85 vs 1.31) were observed in platelet refractoriness group when compared with effective platelet therapy group (P<0.05). However, the significant difference was not observed in B lymphocytes between the two group (3.02% vs 2.85%, P>0.05). Platelet glycoproteins CD41a and CD61 and antibodies were both expressed at high levels in platelet refractoriness group (88.10% vs 51.69%, 88.36% vs 51.83%, 85.37% vs 14.82%, respectively, P<0.05).
  
  
  CONCLUSIONS
  Activation of cytotoxic T cells, suppression of helper T cells, higher expression level of platelet glycoproteins CD41a and CD61 as well as the development of anti-platelet antibodies are involved in the immunologic mechanism of platelet refractoriness.
authors.affiliation: The General Hospital of Tianjin Medical University, Tianjin 300052, China.
authors.forename: C H
authors.initials: CH
authors.lastname: Du
authors.forename: H T
authors.initials: HT
authors.lastname: Huang
authors.forename: G Y
authors.initials: GY
authors.lastname: Yuan
authors.forename: G S
authors.initials: GS
authors.lastname: Zhao
authors.forename: H X
authors.initials: HX
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: Y J
authors.initials: YJ
authors.lastname: Sun
authors.forename: H M
authors.initials: HM
authors.lastname: Xu
authors.forename: S Z
authors.initials: SZ
authors.lastname: Dong
mesh_terms: D001323:Autoantibodies / Q000097:blood*; D001402:B-Lymphocytes / Q000166:cytology; D001792:Blood Platelets / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D016131:Lymphocyte Subsets / Q000166:cytology*; D017713:Platelet Transfusion; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology; D017211:Treatment Failure
chemical_list: D001323:Autoantibodies