title: Polymer mesh scaffold combined with cell-derived ECM for osteogenesis of human mesenchymal stem cells.
abstract: BACKGROUND
  Tissue-engineered scaffold should mimic the structure and biological function of the extracellular matrix and have mechanically supportive properties for tissue regeneration. In this study, we utilized a PLGA/PLA mesh scaffold, coated with cell-derived extracellular matrix (CDM) and assessed its potential as an osteogenic microenvironment for human umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs). CDM was obtained by decellularization of in vitro-cultured type I collagen overexpressing (Col I -293 T-DK) cells. Test groups are mesh itself (control), fibronectin-coated (FN-mesh), and CDM-coated mesh scaffold (CDM-mesh). CDM was then solubilized and used for scaffold coating.
  
  
  RESULTS
  CDM was successfully collected and applied to mesh scaffolds. The presence of CDM was confirmed via SEM and FN immunofluorescence. After then, UCB-MSCs were seeded into the scaffolds and subjected to the induction of osteogenic differentiation for 21 days in vitro. We found that the seeded cells were viable and have better proliferation activity on CDM-mesh scaffold. In addition, when osteogenic differentiation of UCB-MSCs was examined for up to 21 days, alkaline phosphatase (ALP) activity and osteogenic marker (COL I, ALP, osteocalcin, bone sialoprotein) expression were significantly improved with UCB-MSCs when cultured in the CDM-mesh scaffold compared to the control and FN-mesh.
  
  
  CONCLUSIONS
  Polymer mesh scaffold incorporated with CDM can provide UCB-MSCs with a better microenvironment for osteogenesis in vitro.
authors.affiliation: Center for Biomaterials, Korea Institute of Science and Technology, Seoul, 02792 Republic of Korea ; Department of Biomedical Science, Kyung Hee University, Seoul, 02447 Republic of Korea.
authors.forename: Yong Kwan
authors.initials: YK
authors.lastname: Noh
authors.affiliation: Center for Biomaterials, Korea Institute of Science and Technology, Seoul, 02792 Republic of Korea.
authors.forename: Ping
authors.initials: P
authors.lastname: Du
authors.affiliation: Center for Biomaterials, Korea Institute of Science and Technology, Seoul, 02792 Republic of Korea.
authors.forename: In Gul
authors.initials: IG
authors.lastname: Kim
authors.affiliation: Department of Technical application, Korea Institute of Industrial Technology, Gyeonggi, 31056 Republic of Korea.
authors.forename: Jaehoon
authors.initials: J
authors.lastname: Ko
authors.affiliation: Department of Biochemistry and Molecular Biology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, 05505 Republic of Korea.
authors.forename: Seong Who
authors.initials: SW
authors.lastname: Kim
authors.affiliation: Center for Biomaterials, Korea Institute of Science and Technology, Seoul, 02792 Republic of Korea ; Department of Biomedical engineering, University of Science and Technology, Daejeon, 34113 Republic of Korea.
authors.forename: Kwideok
authors.initials: K
authors.lastname: Park
keywords: Cell-derived extracellular matrix (CDM); Microenvironment; Osteogenesis; Polymer mesh scaffold; Umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs)