title: Construction of a metabolomics profile of arsenic trioxide effect in gastric carcinoma cell line SGC7901.
abstract: Arsenic trioxide (ATO) is highly effective for treating acute promyelocytic leukemia. It also holds the promise for treating solid tumors, including gastric carcinoma. However, the molecular mechanism of the effectiveness of ATO to solid tumor is still poorly understood. In this study, we chosed gastric carcinoma as an example and tried to reveal the antitumor mechanism through metabolomics. Gastric carcinoma cell line SGC7901 was treated with ATO for 6, 12, and 24 h. The global metabolite profiles were monitored by metabolomics analysis using gas chromatography (GC)/mass spectrometry (MS) and liquid chromatography/MS/MS. A total of 281 certified metabolites were reliably detected. Bioinformatics analysis showed that glycerophospholipid synthesis, one-carbon synthesis, and glutathione synthesis were affected dramatically. Other cellular functions/pathways that had been affected included inflammatory response, nicotinamide adenine dinucleotide (NAD(+)), and polyamine biosynthesis pathway. The metabolomics data from this study, in combination with previous transcriptomics and proteomics data, could serve as valuable resources for the understanding of the specific antitumor mechanism of ATO treatment.
authors.affiliation: Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China State Key Laboratory of Oncogenes and Related Genes, Shanghai 200240, China School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Ziqing
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Hainan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Integrative Oncology, Fudan University Shanghai Cancer Center, Shanghai 200032, China Central Laboratory, Fudan University Shanghai Cancer Center, Shanghai 200032, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Yang
authors.affiliation: Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Hewei
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Shujuan
authors.initials: S
authors.lastname: Guo
authors.affiliation: Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China State Key Laboratory of Oncogenes and Related Genes, Shanghai 200240, China School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China taosc@sjtu.edu.cn.
authors.forename: Shengce
authors.initials: S
authors.lastname: Tao
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D015317:Biogenic Polyamines / Q000378:metabolism; D045744:Cell Line, Tumor; D016207:Cytokines / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D005978:Glutathione / Q000378:metabolism; D020404:Glycerophospholipids / Q000378:metabolism; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D055442:Metabolome / Q000187:drug effects; D055432:Metabolomics; D009243:NAD / Q000378:metabolism; D010087:Oxides / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D000970:Antineoplastic Agents; D001152:Arsenicals; D015317:Biogenic Polyamines; D016207:Cytokines; D005227:Fatty Acids; D020404:Glycerophospholipids; D018836:Inflammation Mediators; D010087:Oxides; D009243:NAD; D005978:Glutathione; D000077237:Arsenic Trioxide
keywords: arsenic trioxide; gastric carcinoma; metabolomics