title: No association between global DNA methylation in peripheral blood and lung cancer risk in nonsmoking women: results from a multicenter study in Eastern and Central Europe.
abstract: Alterations in global DNA methylation have been suggested to play an important role in cancer development. We evaluated the association of global DNA methylation in peripheral blood with the risk of lung cancer in nonsmoking women from six countries in Central and Eastern Europe. This multicenter case-control study included primary, incident lung cancer cases diagnosed from 1998 to 2001 and controls frequency-matched for geographic area, sex, and age. Global methylation was assessed in peripheral blood DNA from 83 nonsmoking female cases and 181 nonsmoking female controls using the luminometric methylation assay (LUMA). Unconditional logistic regression models were used to estimate associations between DNA methylation in the blood and the risk of lung cancer. LUMA methylation level was not associated with the risk of lung cancer in nonsmoking women. Associations were not significantly different according to different strata of age, BMI, alcohol drinking, or second-hand tobacco smoke exposure status. In our study of nonsmoking women, the LUMA methylation level in peripheral blood was not associated with the risk of lung cancer. Our findings do not support an association of global blood DNA methylation with the risk of lung cancer in nonsmoking women.
authors.affiliation: Department of Biostatistics and Epidemiology, University of North Texas Health Science Center, Fort Worth, Texas.
authors.forename: Ann
authors.initials: A
authors.lastname: Davis
authors.affiliation: Department of Biostatistics and Epidemiology, University of North Texas Health Science Center, Fort Worth, Texas.|Institute for Translational Epidemiology.
authors.forename: Meng-Hua
authors.initials: MH
authors.lastname: Tao
authors.affiliation: Department of Community and Preventive Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors.forename: Jia
authors.initials: J
authors.lastname: Chen
authors.affiliation: International Agency for Research on Cancer, Lyon, France.
authors.forename: Ghislaine
authors.initials: G
authors.lastname: Scelo
authors.affiliation: Institute of Hygiene and Epidemiology, First Faculty of Medicine, Charles University in Prague, Prague.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Bencko
authors.affiliation: Department of Occupational Health, Specialized State Health Institute, Banska Bystrica, Slovakia.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Fabianova
authors.affiliation: Department of Cancer Epidemiology and Genetics, Masaryk Cancer Institute and Medical Faculty of Masaryk University, Brno.
authors.forename: Lenka
authors.initials: L
authors.lastname: Foretova
authors.affiliation: Department of Preventive Medicine, Palacky University of Medicine, Olomouc, Czech Republic.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Janout
authors.affiliation: Department of Cancer Epidemiology and Prevention, Cancer Center and M. Sklodowska-Curie Memorial Institute of Oncology, Warsaw, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Lissowska
authors.affiliation: Institute of Hygiene, Public Health, Health Services and Management.
authors.forename: Dana
authors.initials: D
authors.lastname: Mates
authors.affiliation: General Surgery Department, "St Mary" Clinical Hospital, University of Medicine and Pharmacy 'Carol Davila' Bucharest, Romania.
authors.forename: Ioan N
authors.initials: IN
authors.lastname: Mates
authors.affiliation: National Institute of Environmental Health, Budapest, Hungary.
authors.forename: Peter
authors.initials: P
authors.lastname: Rudnai
authors.affiliation: Department of Epidemiology and Prevention, Russian N.N. Blokhin Cancer Research Center, Moscow, Russia.
authors.forename: David
authors.initials: D
authors.lastname: Zaridze
authors.affiliation: Institute for Translational Epidemiology.
authors.forename: Paolo
authors.initials: P
authors.lastname: Boffetta
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D058621:Blood Buffy Coat; D016022:Case-Control Studies; D019175:DNA Methylation; D005060:Europe; D005260:Female; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D008875:Middle Aged; D000078405:Non-Smokers / Q000706:statistics & numerical data*; D018570:Risk Assessment / Q000379:methods; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor