title: Transcriptional Profiling of the Trichoderma reesei Recombinant Strain HJ48 by RNA-Seq.
abstract: The ethanol production of Trichoderma reesei was improved by genome shuffling in our previous work. Using RNA-Seq, the transcriptomes of T. reesei wild-type CICC40360 and recombinant strain HJ48 were compared under fermentation conditions. Based on this analysis, we defined a set of T. reesei genes involved in ethanol production. Further expression analysis identified a series of glycolysis enzymes, which are upregulated in the recombinant strain HJ48 under fermentation conditions. The differentially expressed genes were further validated by qPCR. The present study will be helpful for future studies on ethanol fermentation as well as the roles of the involved genes. This research reveals several major differences in metabolic pathways between recombinant strain HJ48 and wild-type CICC40360, which relates to the higher ethanol production on the former, and their further research could promote the development of techniques for increasing ethanol production.
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi 530004, P.R. China.|College of Life Science and Technology, Guangxi University, Nanning 530004, P.R. China.|National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, Nanning, Guangxi 530007, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Huang
authors.affiliation: National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, Nanning, Guangxi 530007, P.R. China.
authors.forename: Renzhi
authors.initials: R
authors.lastname: Wu
authors.affiliation: National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, Nanning, Guangxi 530007, P.R. China.
authors.forename: Dong
authors.initials: D
authors.lastname: Chen
authors.affiliation: National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, Nanning, Guangxi 530007, P.R. China.
authors.forename: Qingyan
authors.initials: Q
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi 530004, P.R. China.|College of Life Science and Technology, Guangxi University, Nanning 530004, P.R. China.|National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, Nanning, Guangxi 530007, P.R. China.
authors.forename: Ribo
authors.initials: R
authors.lastname: Huang
mesh_terms: D001692:Biological Transport; D002244:Carbon / Q000378:metabolism; D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods; D004734:Energy Metabolism / Q000235:genetics; D005285:Fermentation; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D063990:Gene Ontology; D005947:Glucose / Q000378:metabolism; D059014:High-Throughput Nucleotide Sequencing; D014176:Protein Biosynthesis / Q000235:genetics; D059467:Transcriptome; D014242:Trichoderma / Q000235:genetics* / Q000378:metabolism
chemical_list: D002244:Carbon; D005947:Glucose
keywords: RNA-Seq; Trichoderma reesei; qPCR; transcriptome