title: Ultrasensitive Visual Detection of HIV DNA Biomarkers via a Multi-amplification Nanoplatform.
abstract: Methodologies to detect disease biomarkers at ultralow concentrations can potentially improve the standard of living. A facile and label-free multi-amplification strategy is proposed for the ultrasensitive visual detection of HIV DNA biomarkers in real physiological media. This multi-amplification strategy not only exhibits a signficantly low detection limit down to 4.8 pM but also provides a label-free, cost-effective and facile technique for visualizing a few molecules of nucleic acid analyte with the naked eye. Importantly, the biosensor is capable of discriminating single-based mismatch lower than 5.0 nM in human serum samples. Moreover, the visual sensing platform exhibits excellent specificity, acceptable reusability and a long-term stability. All these advantages could be attributed to the nanofibrous sensing platform that 1) has a high surface-area-to-volume provided by electrospun nanofibrous membrane, and 2) combines glucose oxidase (GOx) biocatalysis, DNAzyme-catalyzed colorimetric reaction and catalytic hairpin assembly (CHA) recycling amplification together. This multi-amplification nanoplatform promises label-free and visual single-based mismatch DNA monitoring with high sensitivity and specificity, suggesting wide applications that range from virus detection to genetic disease diagnosis.
authors.affiliation: College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu 610064, People's Republic of China.
authors.forename: Yuyin
authors.initials: Y
authors.lastname: Long
authors.affiliation: College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu 610064, People's Republic of China.
authors.forename: Cuisong
authors.initials: C
authors.lastname: Zhou
authors.affiliation: College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu 610064, People's Republic of China.
authors.forename: Congmin
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu 610064, People's Republic of China.
authors.forename: Honglian
authors.initials: H
authors.lastname: Cai
authors.affiliation: College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu 610064, People's Republic of China.
authors.forename: Cuiyun
authors.initials: C
authors.lastname: Yin
authors.affiliation: College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu 610064, People's Republic of China.
authors.forename: Qiufang
authors.initials: Q
authors.lastname: Yang
authors.affiliation: College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu 610064, People's Republic of China.|College of Chemical Engineering, Sichuan University, 29 Wangjiang Road, Chengdu 610065, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Xiao
mesh_terms: D052160:Benzothiazoles; D015415:Biomarkers; D015374:Biosensing Techniques; D003124:Colorimetry; D004279:DNA, Viral / Q000097:blood*; D005240:Feasibility Studies; D054856:G-Quadruplexes; D005949:Glucose Oxidase; D015658:HIV Infections / Q000097:blood*; D006801:Humans; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers; D036103:Nanotechnology / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013451:Sulfonic Acids
chemical_list: D052160:Benzothiazoles; D015415:Biomarkers; D004279:DNA, Viral; D008567:Membranes, Artificial; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D005949:Glucose Oxidase