title: Effects of heavy metals and soil physicochemical properties on wetland soil microbial biomass and bacterial community structure.
abstract: Heavy metals (HMs) contamination is a serious environmental issue in wetland soil. Understanding the micro ecological characteristic of HMs polluted wetland soil has become a public concern. The goal of this study was to identify the effects of HMs and soil physicochemical properties on soil microorganisms and prioritize some parameters that contributed significantly to soil microbial biomass (SMB) and bacterial community structure. Bacterial community structure was analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Relationships between soil environment and microorganisms were analyzed by correlation analysis and redundancy analysis (RDA). The result indicated relationship between SMB and HMs was weaker than SMB and physicochemical properties. The RDA showed all eight parameters explained 74.9% of the variation in the bacterial DGGE profiles. 43.4% (contain the variation shared by Cr, Cd, Pb and Cu) of the variation for bacteria was explained by the four kinds of HMs, demonstrating HMs contamination had a significant influence on the changes of bacterial community structure. Cr solely explained 19.4% (p<0.05) of the variation for bacterial community structure, and Cd explained 17.5% (p<0.05), indicating Cr and Cd were the major factors related to bacterial community structure changes.
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.
authors.forename: Chang
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Nie
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China. Electronic address: liangjie@hnu.edu.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Liang
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.
authors.forename: Guangming
authors.initials: G
authors.lastname: Zeng
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.
authors.forename: Haipeng
authors.initials: H
authors.lastname: Wu
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Hua
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.
authors.forename: Jiayu
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.
authors.forename: Haibing
authors.initials: H
authors.lastname: Xiao
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.
authors.forename: Linjing
authors.initials: L
authors.lastname: Deng
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Xiang
mesh_terms: D018533:Biomass; D004784:Environmental Monitoring; D019216:Metals, Heavy / Q000032:analysis / Q000633:toxicity*; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000633:toxicity*; D053833:Wetlands
chemical_list: D019216:Metals, Heavy; D012987:Soil; D012989:Soil Pollutants
keywords: Bacterial community structure; Heavy metals; Soil microbial biomass; Soil physicochemical properties; Wetland soil