title: Proteome Modification in Tomato Plants upon Long-Term Aluminum Treatment.
abstract: This study aimed to identify the aluminum (Al)-induced proteomes in tomato (Solanum lycopersicum, "Micro-Tom") after long-term exposure to the stress factor. Plants were treated in Magnavaca's solution (pH 4.5) supplemented with 7.5 μM Al(3+) ion activity over a 4 month period beginning at the emergence of flower buds and ending when the lower mature leaves started to turn yellow. Proteomes were identified using a 8-plex isobaric tags for relative and absolute quantification (iTRAQ) labeling strategy followed by a two-dimensional (high- and low-pH) chromatographic separation and final generation of tandem mass spectrometry (MS/MS) spectra of tryptic peptides on an LTQ-Orbitrap Elite mass spectrometer. Principal component analysis revealed that the Al-treatment had induced systemic alterations in the proteomes from roots and leaves but not seed tissues. The significantly changed root proteins were shown to have putative functions in Al(3+) ion uptake and transportation, root development, and a multitude of other cellular processes. Changes in the leaf proteome indicate that the light reaction centers of photosynthetic machinery are the primary targets of Al-induced stress. Embryo and seed-coat tissues derived from Al-treated plants were enriched with stress proteins. The biological processes involving these Al-induced proteins concur with the physiological and morphological changes, such as the disturbance of mineral homeostasis (higher contents of Al, P, and Fe and reduced contents of S, Zn, and Mn in Al-treated compared to nontreated plants) in roots and smaller sizes of roots and the whole plants. More importantly, the identified significant proteins might represent a molecular mechanism for plants to develop toward establishing the Al tolerance and adaptation mechanism over a long period of stress treatment.
authors.affiliation: Department of Agricultural and Environmental Sciences, College of Agriculture, Human and Natural Sciences, Tennessee State University , 3500 John A Merritt Blvd, Nashville, Tennessee 37209, United States.
authors.forename: Suping
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Agricultural and Environmental Sciences, College of Agriculture, Human and Natural Sciences, Tennessee State University , 3500 John A Merritt Blvd, Nashville, Tennessee 37209, United States.
authors.forename: Ikenna
authors.initials: I
authors.lastname: Okekeogbu
authors.affiliation: Department of Agricultural and Environmental Sciences, College of Agriculture, Human and Natural Sciences, Tennessee State University , 3500 John A Merritt Blvd, Nashville, Tennessee 37209, United States.
authors.forename: Sasikiran
authors.initials: S
authors.lastname: Sangireddy
authors.affiliation: Department of Agricultural and Environmental Sciences, College of Agriculture, Human and Natural Sciences, Tennessee State University , 3500 John A Merritt Blvd, Nashville, Tennessee 37209, United States.
authors.forename: Zhujia
authors.initials: Z
authors.lastname: Ye
authors.affiliation: Department of Agricultural and Environmental Sciences, College of Agriculture, Human and Natural Sciences, Tennessee State University , 3500 John A Merritt Blvd, Nashville, Tennessee 37209, United States.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Agricultural and Environmental Sciences, College of Agriculture, Human and Natural Sciences, Tennessee State University , 3500 John A Merritt Blvd, Nashville, Tennessee 37209, United States.
authors.forename: Sarabjit
authors.initials: S
authors.lastname: Bhatti
authors.affiliation: Department of Agricultural and Environmental Sciences, College of Agriculture, Human and Natural Sciences, Tennessee State University , 3500 John A Merritt Blvd, Nashville, Tennessee 37209, United States.
authors.forename: Dafeng
authors.initials: D
authors.lastname: Hui
authors.affiliation: Phenotype Screening Corporation , 4028 Papermill Road, Knoxville, Tennessee 37909, United States.
authors.forename: Daniel W
authors.initials: DW
authors.lastname: McDonald
authors.affiliation: RW Holley Center for Agriculture and Health, Plant, Soil and Nutrition Research Unit, USDA-ARS , Tower Rd, Ithaca, New York 14853, United States.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: RW Holley Center for Agriculture and Health, Plant, Soil and Nutrition Research Unit, USDA-ARS , Tower Rd, Ithaca, New York 14853, United States.
authors.forename: Shree
authors.initials: S
authors.lastname: Giri
authors.affiliation: RW Holley Center for Agriculture and Health, Plant, Soil and Nutrition Research Unit, USDA-ARS , Tower Rd, Ithaca, New York 14853, United States.
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Howe
authors.affiliation: RW Holley Center for Agriculture and Health, Plant, Soil and Nutrition Research Unit, USDA-ARS , Tower Rd, Ithaca, New York 14853, United States.
authors.forename: Tara
authors.initials: T
authors.lastname: Fish
authors.affiliation: RW Holley Center for Agriculture and Health, Plant, Soil and Nutrition Research Unit, USDA-ARS , Tower Rd, Ithaca, New York 14853, United States.
authors.forename: Theodore W
authors.initials: TW
authors.lastname: Thannhauser
mesh_terms: D000222:Adaptation, Physiological; D000535:Aluminum / Q000493:pharmacokinetics / Q000494:pharmacology*; D018551:Solanum lycopersicum / Q000737:chemistry* / Q000196:embryology; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D020543:Proteome / Q000187:drug effects* / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D012639:Seeds / Q000187:drug effects / Q000378:metabolism
chemical_list: D020543:Proteome; D000535:Aluminum
keywords: aluminum stress; embryos; functional pathways; iTRAQ proteomics; leaves; roots; seed coat; tomato