title: Tanshinone ⅡA inhibits human esophageal cancer cell growth through miR-122-mediated PKM2 down-regulation.
abstract: Pyruvate kinase M2 (PKM2) plays a pivotal role in the growth, survival and metabolic reprogramming of cancer cells. Here, we presented for the first time that tanshinone ⅡA inhibited human esophagus cancer cell growth through miR-122-mediated PKM2 down-regulation pathway. Tanshinone ⅡA inhibited cell proliferation and induced cell cycle arrest in S phase in human Ec109 cells. As expected, tanshinone ⅡA down-regulated PKM2 mRNA and protein expression in Ec109 cells. Given these findings, we further investigated microRNAs regulation of PKM2 and confirmed miR-122 for targeting PKM2. Moreover, we found that tanshinone ⅡA-induced up-regulation of miR-122 expression inhibited PKM2 expression in Ec109 cells. Meanwhile, tanshinone ⅡA inhibited proliferation through miR122-medated PKM2 down-regulation. It was demonstrated that the anticancer activity of tanshinone ⅡA was targeted at metabolic regulation of miR-122/PKM2 in human esophagus cancer cells. Taken together, our results revealed tanshinone ⅡA targeting at PKM2-mediated metabolic reprogramming play an important role in inhibition of esophageal cancer cell growth.
authors.affiliation: College of Life Science & Bioengineering, Beijing University of Technology, Pingleyuan 100#, District of Chaoyang, Beijing, 100124, China. Electronic address: zhanghs@bjut.edu.cn.
authors.forename: Hong-Sheng
authors.initials: HS
authors.lastname: Zhang
authors.affiliation: College of Life Science & Bioengineering, Beijing University of Technology, Pingleyuan 100#, District of Chaoyang, Beijing, 100124, China.
authors.forename: Feng-Juan
authors.initials: FJ
authors.lastname: Zhang
authors.affiliation: College of Life Science & Bioengineering, Beijing University of Technology, Pingleyuan 100#, District of Chaoyang, Beijing, 100124, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Life Science & Bioengineering, Beijing University of Technology, Pingleyuan 100#, District of Chaoyang, Beijing, 100124, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Life Science & Bioengineering, Beijing University of Technology, Pingleyuan 100#, District of Chaoyang, Beijing, 100124, China.
authors.forename: Guang-Yuan
authors.initials: GY
authors.lastname: Du
authors.affiliation: College of Life Science & Bioengineering, Beijing University of Technology, Pingleyuan 100#, District of Chaoyang, Beijing, 100124, China.
authors.forename: Ying-Hui
authors.initials: YH
authors.lastname: Huang
mesh_terms: D045784:Abietanes / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D015536:Down-Regulation / Q000187:drug effects*; D004938:Esophageal Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis*; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D011770:Pyruvate Kinase / Q000096:biosynthesis*; D012334:RNA, Neoplasm / Q000096:biosynthesis*
chemical_list: D045784:Abietanes; D000970:Antineoplastic Agents; C531907:MIRN122 microRNA, human; D035683:MicroRNAs; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; C021751:tanshinone; D011770:Pyruvate Kinase
keywords: Metabolic reprogramming; PKM2; Tanshinone ⅡA; miR-122