title: MicroRNA-495 Regulates Migration and Invasion in Prostate Cancer Cells Via Targeting Akt and mTOR Signaling.
abstract: Abnormal microRNA (miR) expressions were implicated in prostate cancer progression. We identified a novel miR-495, which was downregulated in prostate cancer, but not normal prostate cell lines. MiR-495 directly targeted the 3'-UTR of Akt and mTOR mRNAs. Expression of miR-495 in prostate cancer cells significantly downregulated Akt and mTOR, which further inhibited cancer cell proliferation, migration, and invasion in vitro. Function of miR-495 in vivo was examined in mouse xenograft model and was found to significantly inhibit the growth of tumors, mediated by repressing Akt and mTOR. Our report supported miR-495 as a novel tumor suppressor microRNA in prostate cancer.
authors.affiliation: a Department of Urology , The Fourth Affiliated Hospital of Harbin Medical University , Harbin , China.
authors.forename: Jian-Zhang
authors.initials: JZ
authors.lastname: Li
authors.affiliation: b Department of Urology , The Second Affiliated Hospital of Xi'an Jiaotong University , Xi'an , China.
authors.forename: Zhen-Long
authors.initials: ZL
authors.lastname: Wang
authors.affiliation: a Department of Urology , The Fourth Affiliated Hospital of Harbin Medical University , Harbin , China.
authors.forename: Wan-Hai
authors.initials: WH
authors.lastname: Xu
authors.affiliation: a Department of Urology , The Fourth Affiliated Hospital of Harbin Medical University , Harbin , China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.affiliation: a Department of Urology , The Fourth Affiliated Hospital of Harbin Medical University , Harbin , China.
authors.forename: Lin
authors.initials: L
authors.lastname: Gao
authors.affiliation: b Department of Urology , The Second Affiliated Hospital of Xi'an Jiaotong University , Xi'an , China.
authors.forename: Zi-Ming
authors.initials: ZM
authors.lastname: Wang
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D015536:Down-Regulation; D006367:HeLa Cells; D064593:Heterografts; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D058570:TOR Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; C577768:MIRN495 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; C546842:MTOR protein, human; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases
keywords: Akt; Mammalian target of rapamycin (mTOR); MicroRNA-495; Prostate cancer; Tumorigenesis