title: Probiotic Strain Bifidobacterium animalis subsp. lactis CECT 8145 Reduces Fat Content and Modulates Lipid Metabolism and Antioxidant Response in Caenorhabditis elegans.
abstract: Recently, microbial changes in the human gut have been proposed as a possible cause of obesity. Therefore, modulation of microbiota through probiotic supplements is of great interest to support obesity therapeutics. The present study examines the functional effect and metabolic targets of a bacterial strain, Bifidobacterium animalis subsp. lactis CECT 8145, selected from a screening in Caenorhabditis elegans. This strain significantly reduced total lipids (40.5% ± 2.4) and triglycerides (27.6% ± 0.5), exerting antioxidant effects in the nematode (30% ± 2.8 increase in survival vs control); activities were also preserved in a final food matrix (milk). Furthermore, transcriptomic and metabolomic analyses in nematodes fed with strain CECT 8145 revealed modulation of the energy and lipid metabolism, as well as the tryptophan metabolism (satiety), as the main metabolic targets of the probiotic. In conclusion, our study describes for the first time a new B. animalis subsp. lactis strain, CECT 8145, as a promising probiotic for obesity disorders. Furthermore, the data support future studies in obesity murine models.
authors.affiliation: Cell Biology Laboratory, Food Biotechnology Department, Biópolis SL , Paterna, 46980 Valencia, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: Martorell
authors.affiliation: Cell Biology Laboratory, Food Biotechnology Department, Biópolis SL , Paterna, 46980 Valencia, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Llopis
authors.affiliation: Cell Biology Laboratory, Food Biotechnology Department, Biópolis SL , Paterna, 46980 Valencia, Spain.
authors.forename: Nuria
authors.initials: N
authors.lastname: González
authors.affiliation: Cell Biology Laboratory, Food Biotechnology Department, Biópolis SL , Paterna, 46980 Valencia, Spain.
authors.forename: Empar
authors.initials: E
authors.lastname: Chenoll
authors.affiliation: Department of Pharmacology, School of Medicine, Complutense University of Madrid , Avda. Complutense s/n, 28040 Madrid, Spain.
authors.forename: Noemi
authors.initials: N
authors.lastname: López-Carreras
authors.affiliation: Department of Pharmacology, School of Medicine, Complutense University of Madrid , Avda. Complutense s/n, 28040 Madrid, Spain.
authors.forename: Amaya
authors.initials: A
authors.lastname: Aleixandre
authors.affiliation: Metabolon Inc. , Durham, North Carolina 27713, United States.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Metabolon Inc. , Durham, North Carolina 27713, United States.
authors.forename: Edwuard D
authors.initials: ED
authors.lastname: Karoly
authors.affiliation: Cell Biology Laboratory, Food Biotechnology Department, Biópolis SL , Paterna, 46980 Valencia, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Ramón
authors.affiliation: Cell Biology Laboratory, Food Biotechnology Department, Biópolis SL , Paterna, 46980 Valencia, Spain.
authors.forename: Salvador
authors.initials: S
authors.lastname: Genovés
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001644:Bifidobacterium / Q000502:physiology*; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D050356:Lipid Metabolism; D055432:Metabolomics; D019936:Probiotics
chemical_list: D000975:Antioxidants
keywords: Bifidobacterium strain; Caenorhabditis elegans; metabolic syndrome; obesity; probiotic