title: Responses of soil microbial biomass and bacterial community structure to closed-off management (an ecological natural restoration measures): A case study of Dongting Lake wetland, middle China.
abstract: Soil microbial biomass (SMB) and bacterial community structure, which are critical to global ecosystem and fundamental ecological processes, are sensitive to anthropogenic activities and environmental conditions. In this study, we examined the possible effects of closed-off management (an ecological natural restoration measures, ban on anthropogenic activity, widely employed for many important wetlands) on SMB, soil bacterial community structure and functional marker genes of nitrogen cycling in Dongting Lake wetland. Soil samples were collected from management area (MA) and contrast area (CA: human activities, such as hunting, fishing and draining, are permitted) in November 2013 and April 2014. Soil properties, microbial biomass carbon (MBC), and bacterial community structure were investigated. Comparison of the values of MA and CA showed that SMB and bacterial community diversity of the MA had a significant increase after 7 years closed-off management. The mean value of Shannon-Weiner diversity index of MA and CA respectively were 2.85 and 2.07. The gene copy numbers of 16S rRNA and nosZ of MA were significant higher than those of CA. the gene copy numbers of ammonia-oxidizing archaea (AOA) and nirK of MA were significant lower than those of CA. However, there was no significant change in the gene copy numbers of ammonia-oxidizing bacteria (AOB) and nirS.
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China; Changjiang River Scientific Research Institute, Wuhan 430072, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Dai
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China; Changjiang River Scientific Research Institute, Wuhan 430072, PR China.
authors.forename: Haipeng
authors.initials: H
authors.lastname: Wu
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.
authors.forename: Chang
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China. Electronic address: zgming@hnu.edu.cn.
authors.forename: Guangming
authors.initials: G
authors.lastname: Zeng
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liang
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; State Key Laboratory of Water Resources and Hydropower Engineering Science, Wuhan 430072, PR China.
authors.forename: Shenglian
authors.initials: S
authors.lastname: Guo
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Huang
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Reservoir Aquatic Environment of CAS, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China.
authors.forename: Lunhui
authors.initials: L
authors.lastname: Lu
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Yuan
mesh_terms: D000641:Ammonia / Q000378:metabolism; D001105:Archaea / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification; D018533:Biomass; D002681:China; D005798:Genes, Bacterial / Q000235:genetics; D006802:Human Activities; D060106:Lakes; D058458:Nitrogen Cycle / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D053833:Wetlands
chemical_list: D012336:RNA, Ribosomal, 16S; D012987:Soil; D000641:Ammonia
keywords: Bacterial community diversity; Ecological management; Ecological restoration; Microbial biomass carbon; Nitrogen; Wetland