title: Effect of miR-146a/bFGF/PEG-PEI Nanoparticles on Inflammation Response and Tissue Regeneration of Human Dental Pulp Cells.
abstract: BACKGROUND
  Inflammation in dental pulp cells (DPCs) initiated by Lipopolysaccharide (LPS) results in dental pulp necrosis. So far, whether there is a common system regulating inflammation response and tissue regeneration remains unknown. miR-146a is closely related to inflammation. Basic fibroblast growth factor (bFGF) is an important regulator for differentiation.
  
  
  METHODS
  To explore the effect of miR-146a/bFGF on inflammation and tissue regeneration, polyethylene glycol-polyethyleneimine (PEG-PEI) was synthesized, and physical characteristics were analyzed by dynamic light scattering and gel retardation analysis. Cell absorption, transfection efficiency, and cytotoxicity were assessed. Alginate gel was combined with miR-146a/PEG-PEI nanoparticles and bFGF. Drug release ratio was measured by ultraviolet spectrophotography. Proliferation and odontogenic differentiation of DPCs with 1 μg/mL LPS treatment were determined. Results. PEG-PEI prepared at N/P 2 showed complete gel retardation and smallest particle size and zeta potential. Transfection efficiency of PEG-PEI was higher than lipo2000. Cell viability decreased as N/P ratio increased. Drug release rate amounted to 70% at the first 12 h and then maintained slow release afterwards. Proliferation and differentiation decreased in DPCs with LPS treatment, whereas they increased in miR-146a/bFGF gel group.
  
  
  CONCLUSIONS
  PEG-PEI is a promising vector for gene therapy. miR-146a and bFGF play critical roles in inflammation response and tissue regeneration of DPCs.
authors.affiliation: Operative Dentistry and Endodontics, Guanghua School of Stomatology, Affiliated Stomatological Hospital, Guangdong Province Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong 510055, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.affiliation: Operative Dentistry and Endodontics, Guanghua School of Stomatology, Affiliated Stomatological Hospital, Guangdong Province Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong 510055, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Shu
authors.affiliation: Conservative Dentistry, School of Dentistry, The University of Hong Kong, Pok Fu Lam, Hong Kong.
authors.forename: Gary Shunpan
authors.initials: GS
authors.lastname: Cheung
authors.affiliation: Operative Dentistry and Endodontics, Guanghua School of Stomatology, Affiliated Stomatological Hospital, Guangdong Province Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong 510055, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Wei
mesh_terms: D000293:Adolescent; D000328:Adult; D049109:Cell Proliferation / Q000187:drug effects; D003782:Dental Pulp / Q000166:cytology*; D004337:Drug Carriers / Q000737:chemistry; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D006801:Humans; D008070:Lipopolysaccharides / Q000009:adverse effects; D035683:MicroRNAs / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D053758:Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry; D011671:Pulpitis / Q000139:chemically induced / Q000378:metabolism*; D012038:Regeneration / Q000187:drug effects*; D055815:Young Adult
chemical_list: D004337:Drug Carriers; D008070:Lipopolysaccharides; C542542:MIRN146 microRNA, human; D035683:MicroRNAs; D016222:Fibroblast Growth Factor 2; D011092:Polyethylene Glycols; D011094:Polyethyleneimine