title: ADRB2 Polymorphism and Salbutamol Responsiveness in Northern Indian Children with Mild to Moderate Exacerbation of Asthma.
abstract: OBJECTIVE
  The primary objective was to determine the association between beta-2 adrenergic receptor (ADRB2) gene polymorphism (rs1042713, c.46A>G, p.Arg16Gly) and the response to inhaled salbutamol in North Indian children aged 5 to 15 years, with mild to moderate exacerbation of asthma.
  
  
  METHODS
  This cross-sectional study was done at a tertiary-care hospital in Northern India from June 2011 to May 2013. 120 children with asthma with mild to moderate exacerbation underwent spirometry at baseline and after administration of three doses of salbutamol. An increase in FEV1 =15% was considered as positive response. Blood samples from these children were analysed for ADRB2 polymorphism (p.Arg16Gly). 94 non-asthmatic adult controls were also studied to determine the prevalence of ADRB2 polymorphism.
  
  
  RESULTS
  In asthmatic children, the frequency of AA, GG, AG genotypes were 24.2%, 24.2% and 51.7% compared to 20.2%, 20.2% and 59.6%, respectively in the non-asthmatic adults. Salbutamol responsiveness showed no correlation with the studied ADRB2 polymorphism (p= 0.55). A trend towards greater bronchodilator responsiveness amongst AA genotype, compared to GG genotype was observed (Median change in percent predicted FEV1 14.5% and 7.5%, respectively).
  
  
  CONCLUSIONS
  No correlation was found between salbutamol responsiveness and ADRB2 genotype in Northern Indian children with asthma with mild-to moderate exacerbation.
authors.affiliation: Departments of Pediatrics and #Biostatistics, All India Institute of Medical Sciences, New Delhi, India. Correspondence to: Dr Madhulika Kabra, Professor, Division of Genetics, Department of Pediatrics, All India Institute of Medical Sciences, New Delhi, India. madhulikakabra@hotmail.com.
authors.forename: Puneet Kaur
authors.initials: PK
authors.lastname: Sahi
authors.forename: Shivaram
authors.initials: S
authors.lastname: Shastri
authors.forename: Rakesh
authors.initials: R
authors.lastname: Lodha
authors.forename: Neerja
authors.initials: N
authors.lastname: Gupta
authors.forename: R M
authors.initials: RM
authors.lastname: Pandey
authors.forename: Sushil Kumar
authors.initials: SK
authors.lastname: Kabra
authors.forename: Madhulika
authors.initials: M
authors.lastname: Kabra
mesh_terms: D000293:Adolescent; D000420:Albuterol / Q000627:therapeutic use*; D001249:Asthma / Q000188:drug therapy / Q000453:epidemiology / Q000235:genetics; D001993:Bronchodilator Agents / Q000627:therapeutic use*; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D006801:Humans; D007194:India; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics*
chemical_list: C553443:ADRB2 protein, human; D001993:Bronchodilator Agents; D018343:Receptors, Adrenergic, beta-2; D000420:Albuterol