title: Virulence genes detection of Salmonella serovars isolated from pork and slaughterhouse environment in Ahmedabad, Gujarat.
abstract: OBJECTIVE
  The aim was to detect virulence gene associated with the Salmonella serovars isolated from pork and Slaughterhouse environment.
  
  
  METHODS
  Salmonella isolates (n=37) used in this study were isolated from 270 pork and slaughter house environmental samples collected from the Ahmedabad Municipal Corporation Slaughter House, Ahmedabad, Gujarat, India. Salmonella serovars were isolated and identified as per BAM USFDA method and serotyped at National Salmonella and Escherichia Centre, Central Research Institute, Kasauli (Himachal Pradesh, India). Polymerase chain reaction technique was used for detection of five genes, namely invA, spvR, spvC, fimA and stn among different serovars of Salmonella.
  
  
  RESULTS
  Out of a total of 270 samples, 37 (13.70%) Salmonella were isolated with two serovars, namely Enteritidis and Typhimurium. All Salmonella serovars produced 284 bp invA gene, 84 bp fimA and 260 bp amplicon for enterotoxin (stn) gene whereas 30 isolates possessed 310 bp spvR gene, but no isolate possessed spvC gene.
  
  
  CONCLUSIONS
  Presence of invA, fimA and stn gene in all isolates shows that they are the specific targets for Salmonella identification and are capable of producing gastroenteric illness to humans, whereas 20 Typhimurium serovars and 10 Enteritidis serovars can able to produce systemic infection.
authors.affiliation: Department of Veterinary Public Health, College of Veterinary Science and Animal Husbandry, AAU, Anand - 388 001, Gujarat, India.
authors.forename: J H
authors.initials: JH
authors.lastname: Chaudhary
authors.affiliation: Department of Veterinary Public Health, College of Veterinary Science and Animal Husbandry, AAU, Anand - 388 001, Gujarat, India.
authors.forename: J B
authors.initials: JB
authors.lastname: Nayak
authors.affiliation: Department of Veterinary Public Health, College of Veterinary Science and Animal Husbandry, AAU, Anand - 388 001, Gujarat, India.
authors.forename: M N
authors.initials: MN
authors.lastname: Brahmbhatt
authors.affiliation: Department of Veterinary Public Health, College of Veterinary Science and Animal Husbandry, AAU, Anand - 388 001, Gujarat, India.
authors.forename: P P
authors.initials: PP
authors.lastname: Makwana
keywords: Salmonella; pork; slaughterhouse environment; virulence genes