title: Isoptericola cucumis sp. nov., isolated from the root tissue of cucumber (Cucumis sativus).
abstract: A Gram-stain-positive, aerobic organism, showing an irregular cell morphology, was isolated from the root tissue of cucumber (Cucumis sativus) and investigated in detail for its taxonomic position. On the basis of the 16S rRNA gene sequence analysis, strain AP-38T was shown to be most closely related to Isoptericola variabilis (99.1 %) and Isoptericola nanjingensis (98.9 %). The 16S rRNA gene sequence similarity to all other species of the genus Isoptericola was ≤98.5 %. DNA-DNA relatedness to Isoptericola variablis DSM 10177T and Isoptericola nanjingensis DSM 24300T was 31(reciprocal 41 %) and 34 (reciprocal 34 %), respectively. The diagnostic diamino acid of the peptidoglycan was l-lysine. The quinone system contained predominantly menaquinones MK-9(H4) and MK-9(H2). In the polar lipid profile, major compounds were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two phosphatidylinositol mannosides. The polyamine pattern contained the major components spermidine and spermine and significant amounts of tyramine. In the fatty acid profile, anteiso-C15 : 0 and iso-C15 : 0 were present in major amounts. These data support the allocation of the strain to the genus Isoptericola. The results of physiological and biochemical characterization additionally provide phenotypic differentiation of strain AP-38T from I. variabilis and I. nanjingensis. AP-38T represents a novel species of the genus Isoptericola, for which we propose the name Isoptericola cucumis sp. nov., with AP-38T (= LMG 29223T=CCM 8653T) as the type strain.
authors.affiliation: Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Kämpfer
authors.affiliation: Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
authors.forename: Stefanie P
authors.initials: SP
authors.lastname: Glaeser
authors.affiliation: Department of Entomology and Plant Pathology, Auburn University, Alabama 36849, USA.
authors.forename: Joseph W
authors.initials: JW
authors.lastname: Kloepper
authors.affiliation: Department of Entomology and Plant Pathology, Auburn University, Alabama 36849, USA.
authors.forename: Chia-Hui
authors.initials: CH
authors.lastname: Hu
authors.affiliation: Department of Entomology and Plant Pathology, Auburn University, Alabama 36849, USA.
authors.forename: John A
authors.initials: JA
authors.lastname: McInroy
authors.affiliation: Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V., Hans-Knöll-Institut-, D-07745 Jena, Germany.
authors.forename: Karin
authors.initials: K
authors.lastname: Martin
authors.affiliation: Institut für Mikrobiologie, Veterinärmedizinische Universität, A-1210 Wien, Austria.
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Busse
mesh_terms: D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D018553:Cucumis sativus / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D011073:Polyamines / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D011073:Polyamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9