title: The Klebsiella pneumoniae YfgL (BamB) lipoprotein contributes to outer membrane protein biogenesis, type-1 fimbriae expression, anti-phagocytosis, and in vivo virulence.
abstract: Klebsiella pneumoniae is an opportunistic pathogen that causes several kinds of infections, including pneumonia, bacteremia, urinary tract infection and community-acquired pyogenic liver abscess (PLA). Adhesion is the critical first step in the infection process. Our previous work demonstrated that the transcellular translocation is exploited by K. pneumoniae strains to migrate from the gut flora into other tissues, resulting in systemic infections. However, the initial stages of K. pneumoniae infection remain unclear. In this study, we demonstrated that a K. pneumoniae strain deleted for yfgL (bamB) exhibited reduced adherence to and invasion of host cells; changed biogenesis of major β-barrel outer membrane proteins; decreased transcriptional expression of type-1 fimbriae; and increased susceptibility to vancomycin and erythromycin. The yfgL deletion mutant also had reduced ability to against neutrophil phagocytosis; exhibited decreased induction of host IL-6 production; and was profoundly attenuated for virulence in a K. pneumoniae model of bacteremia. Thus, the K. pneumoniae YfgL lipoprotein mediates in outer membrane proteins biogenesis and is crucial for anti-phagocytosis and survival in vivo. These data provide a new insight for K. pneumoniae attachment and such knowledge could facilitate preventive therapies or alternative therapies against K. pneumoniae.
authors.affiliation: a Department of Microbiology , National Taiwan University College of Medicine , Taipei , Taiwan.
authors.forename: Pei-Fang
authors.initials: PF
authors.lastname: Hsieh
authors.affiliation: b Department of Medical Research , E-Da Hospital , Kaohsiung , Taiwan.|c School of Medicine, I-Shou University , Kaohsiung , Taiwan.
authors.forename: Chun-Ru
authors.initials: CR
authors.lastname: Hsu
authors.affiliation: a Department of Microbiology , National Taiwan University College of Medicine , Taipei , Taiwan.
authors.forename: Chun-Tang
authors.initials: CT
authors.lastname: Chen
authors.affiliation: a Department of Microbiology , National Taiwan University College of Medicine , Taipei , Taiwan.
authors.forename: Tzu-Lung
authors.initials: TL
authors.lastname: Lin
authors.affiliation: a Department of Microbiology , National Taiwan University College of Medicine , Taipei , Taiwan.|d Department of Internal Medicine , National Taiwan University Hospital , Taipei , Taiwan.
authors.forename: Jin-Town
authors.initials: JT
authors.lastname: Wang
mesh_terms: D000818:Animals; D016470:Bacteremia; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D004917:Erythromycin / Q000494:pharmacology; D010861:Fimbriae, Bacterial / Q000235:genetics*; D015850:Interleukin-6 / Q000276:immunology; D007710:Klebsiella Infections / Q000276:immunology / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000737:chemistry / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*; D008074:Lipoproteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D009504:Neutrophils / Q000382:microbiology; D010587:Phagocytosis; D014176:Protein Biosynthesis; D017384:Sequence Deletion; D014640:Vancomycin / Q000494:pharmacology; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D015850:Interleukin-6; D008074:Lipoproteins; D037521:Virulence Factors; D004917:Erythromycin; D014640:Vancomycin
keywords: YfgL (BamB) lipoprotein; adherence; anti-phagocytosis; klebsiella pneumoniae; type-1 fimbriae; virulence