title: IL-1β and IL-18 inhibition of HIV-1 replication in Jurkat cells and PBMCs.
abstract: HIV-1 infection-induced apoptosis is able to ensure viral replication. The death of some CD4+ T cells residing in lymphoid tissues can be induced by HIV-1 infection through caspase-1 driven pyroptosis with release of cytokine of IL-1β and IL-18. It is not well known whether IL-1β and IL-18 affect HIV-1 replication in lymphocytic cells. Using susceptible lymphocytic cell line, Jurkat cells, and primary peripheral blood mononuclear cells (PBMCs), we studied the effects of IL-1β and IL-18 on HIV-1 replication. We found that treatment with exogenous IL-1β protein (rIL-1β) and IL-18 protein (rIL-18), or expression of IL-1β and IL-18 significantly reduced HIV-1 replication. HIV-1 infection enhanced caspase-3 expression and its activation, and had no effects on caspase-1 activity. Treatment with rIL-1β and rIL-18 dramatically lowered caspase-3 activity. IL-1β and IL-18 also played roles in diminishing reactivation of viral replication from latency in J1.1 cells. These results indicate that IL-1β and IL-18 are able to inhibit HIV-1 replication, and their effects may be due to signaling through apoptosis involved in inactivation of caspase-3 activity.
authors.affiliation: Lab of Molecular Virology, Division of Emerging and Transfusion Transmitted Diseases, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD 20993, USA. Electronic address: xue.wang@fda.hhs.gov.
authors.forename: Xue
authors.initials: X
authors.lastname: Wang
authors.affiliation: Lab of Molecular Virology, Division of Emerging and Transfusion Transmitted Diseases, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD 20993, USA.
authors.forename: Christelle
authors.initials: C
authors.lastname: Mbondji-Wonje
authors.affiliation: Lab of Molecular Virology, Division of Emerging and Transfusion Transmitted Diseases, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD 20993, USA.
authors.forename: Jiangqin
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Lab of Molecular Virology, Division of Emerging and Transfusion Transmitted Diseases, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD 20993, USA. Electronic address: indira.hewlett@fda.hhs.gov.
authors.forename: Indira
authors.initials: I
authors.lastname: Hewlett
mesh_terms: D053148:Caspase 3 / Q000378:metabolism; D002478:Cells, Cultured; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D020382:Interleukin-18 / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D019169:Jurkat Cells; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000821:virology*; D014779:Virus Replication
chemical_list: D020382:Interleukin-18; D053583:Interleukin-1beta; D053148:Caspase 3
keywords: Caspase-1; Caspase-3; HIV-1; IL-18; IL-1β; Replication