title: Light and temperature effects on bioactivity in diatoms.
abstract: Isolates of five pelagic North Atlantic marine diatoms (Bacillariophyceae):Attheya longicornis,Chaetoceros socialis,Chaetoceros furcellatus,Skeletonema marinoiandPorosira glacialiswere cultivated in large photobioreactors at two light and two temperature regimes to test if this affected bioactivity. We screened for bioactivity in assays representing five different therapeutic areas: diabetes II (PTP1b), cancer (melanoma cells, A2058), anti-oxidants (FRAP), immunomodulation (TNFa) and anti-infection (MRSA,Enterococcus faecalis,Staphylococcus aureus,Escherichia coliandPseudomonas aeruginosa). All the diatom strains showed activity in two or more assays. We detected differences in bioactivity both between species and within species cultivated with different light and temperature regimes. Our results demonstrate the potential for a more exhaustive exploitation of diatom metabolites that can be obtained by manipulation of the cultivation conditions.
authors.affiliation: Department of Arctic and Marine Biology, Faculty of Biosciences, Fisheries and Economics, UiT - The Arctic University of Norway, Tromsø, N-9037 Norway.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Ingebrigtsen
authors.affiliation: Marbio, UiT - The Arctic University of Norway, Tromsø, N-9037 Norway.
authors.forename: Espen
authors.initials: E
authors.lastname: Hansen
authors.affiliation: Marbio, UiT - The Arctic University of Norway, Tromsø, N-9037 Norway.
authors.forename: Jeanette Hammer
authors.initials: JH
authors.lastname: Andersen
authors.affiliation: Department of Arctic and Marine Biology, Faculty of Biosciences, Fisheries and Economics, UiT - The Arctic University of Norway, Tromsø, N-9037 Norway.
authors.forename: Hans Christian
authors.initials: HC
authors.lastname: Eilertsen
keywords: Bioactivity; Diatoms; OSMAC method