title: Regulation of Macrophage Recognition through the Interplay of Nanoparticle Surface Functionality and Protein Corona.
abstract: Using a family of cationic gold nanoparticles (NPs) with similar size and charge, we demonstrate that proper surface engineering can control the nature and identity of protein corona in physiological serum conditions. The protein coronas were highly dependent on the hydrophobicity and arrangement of chemical motifs on NP surface. The NPs were uptaken in macrophages in a corona-dependent manner, predominantly through recognition of specific complement proteins in the NP corona. Taken together, this study shows that surface functionality can be used to tune the protein corona formed on NP surface, dictating the interaction of NPs with macrophages.
authors.affiliation: Department of Chemistry, University of Massachusetts Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Krishnendu
authors.initials: K
authors.lastname: Saha
authors.affiliation: Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen , Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.|Lehrstuhl für Biotechnologie, RWTH Aachen University , Worringerweg 3, 52074 Aachen, Germany.
authors.forename: Mehran
authors.initials: M
authors.lastname: Rahimi
authors.affiliation: Department of Chemistry, University of Massachusetts Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Mahdieh
authors.initials: M
authors.lastname: Yazdani
authors.affiliation: Department of Chemistry, University of Massachusetts Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Sung Tae
authors.initials: ST
authors.lastname: Kim
authors.affiliation: Department of Chemistry, University of Massachusetts Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Moyano
authors.affiliation: Department of Chemistry, University of Massachusetts Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Singyuk
authors.initials: S
authors.lastname: Hou
authors.affiliation: Department of Chemistry, University of Massachusetts Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Ridhha
authors.initials: R
authors.lastname: Das
authors.affiliation: Department of Chemistry, University of Massachusetts Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Rubul
authors.initials: R
authors.lastname: Mout
authors.affiliation: University Medical Center Groningen (UMCG), University of Groningen , 9713 AV Groningen, The Netherlands.
authors.forename: Farhad
authors.initials: F
authors.lastname: Rezaee
authors.affiliation: Nanotechnology Research Center and Department of Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences , Tehran, 14155-6451, Iran.|Department of Anesthesiology, Brigham and Women's Hospital, Harvard Medical School , Boston, Massachusetts 02115, United States.
authors.forename: Morteza
authors.initials: M
authors.lastname: Mahmoudi
authors.affiliation: Department of Chemistry, University of Massachusetts Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Vincent M
authors.initials: VM
authors.lastname: Rotello
mesh_terms: D000818:Animals; D002412:Cations; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008264:Macrophages / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D010316:Particle Size; D011485:Protein Binding; D000066970:Protein Corona / Q000378:metabolism*; D000067996:RAW 264.7 Cells; D013499:Surface Properties
chemical_list: D002412:Cations; D000066970:Protein Corona; D006046:Gold
keywords: complement system; gold nanoparticles; macrophages; protein corona; surface functionality