title: Clg2p interacts with Clf and ClUrase to regulate appressorium formation, pathogenicity and conidial morphology in Curvularia lunata.
abstract: Ras is a small GTPase that regulates numerous processes in the cellular development and morphogenesis of many organisms. In this study, we identified and functionally characterized the Clg2p gene of Curvularia lunata, which is homologous with the Ras protein. The Clg2p deletion mutant (ΔClg2p) had altered appressorium formation and conidial morphology and produced fewer, smaller lesions compared with the wild-type strain. When a dominant Clg2p allele was introduced into the mutant, all of these defective phenotypes were completely restored. To further understand the regulation of Clg2p in appressorium formation and conidial morphology, and its role in pathogenicity, seven Clg2p-interacting proteins were screened using a yeast two-hybrid assay. Two of these proteins, Clf, a homologue of Mst11, which corresponds to MAP kinase kinase kinase in Magnaporthe oryzae, and urate oxidase (designated ClUrase) were functionally characterized. Clg2p specifically interacted with Clf through its RA domain to regulate appressorium formation and pathogenicity, whereas the Clg2p-ClUrase interaction regulated conidial morphology without affecting fungal pathogenicity. This report is the first to elucidate the regulatory mechanism of the key Ras protein Clg2p in C. lunata.
authors.affiliation: Institute of Plant Pathology and Applied Microbiology, School of Agronomy, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, P. R. China.|State Key Laboratory of Crop Stress Biology in Arid Regions, Northwest A &F University, Yangling, Shanxi, 712100, P. R. China.
authors.forename: Tong
authors.initials: T
authors.lastname: Liu
authors.affiliation: Institute of Plant Pathology and Applied Microbiology, School of Agronomy, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, P. R. China.
authors.forename: Yuying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Plant Pathology and Applied Microbiology, School of Agronomy, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, P. R. China.
authors.forename: Bingchen
authors.initials: B
authors.lastname: Ma
authors.affiliation: Institute of Plant Pathology and Applied Microbiology, School of Agronomy, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, P. R. China.
authors.forename: Jumei
authors.initials: J
authors.lastname: Hou
authors.affiliation: Institute of Plant Pathology and Applied Microbiology, School of Agronomy, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, P. R. China.
authors.forename: Yazhong
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Institute of Plant Pathology and Applied Microbiology, School of Agronomy, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, P. R. China.
authors.forename: Youli
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: National Coarse Cereals Engineering Research Center, Daqing, Heilongjiang, 163319, P. R. China.
authors.forename: Xiwang
authors.initials: X
authors.lastname: Ke
authors.affiliation: Institute of Plant Pathology and Applied Microbiology, School of Agronomy, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, P. R. China.
authors.forename: Lianmei
authors.initials: L
authors.lastname: Tai
authors.affiliation: Institute of Plant Pathology and Applied Microbiology, School of Agronomy, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, P. R. China.
authors.forename: Yuhu
authors.initials: Y
authors.lastname: Zuo
authors.affiliation: Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, 3190 Maile Way, Honolulu, HI, 96822, USA.
authors.forename: Kishore
authors.initials: K
authors.lastname: Dey
mesh_terms: D001203:Ascomycota / Q000378:metabolism / Q000472:pathogenicity* / Q000502:physiology*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D020930:MAP Kinase Kinase Kinases / Q000378:metabolism; D009154:Mutation; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D013172:Spores, Fungal / Q000502:physiology*; D020798:Two-Hybrid System Techniques; D014503:Urate Oxidase / Q000378:metabolism; D014774:Virulence; D018631:ras Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D014503:Urate Oxidase; D020930:MAP Kinase Kinase Kinases; D018631:ras Proteins