title: Modification of the classical Lancefield assay of group A streptococcal killing to reduce inter-donor variation.
abstract: The lack of a surrogate-of-immunity assay presents a major barrier to Streptococcus pyogenes research. Modification of the Lancefield assay to include an antibody digestion step reduced inter-donor variation and permitted detection of the anti-streptococcal activity of intravenous immunoglobulin and convalescent serum, thus facilitating retrospective evaluation of immunity using stored samples.
authors.affiliation: Faculty of Medicine, Imperial College London, Hammersmith Campus, Du Cane Road, London W12 0NN, United Kingdom. Electronic address: m.reglinski10@imperial.ac.uk.
authors.forename: Mark
authors.initials: M
authors.lastname: Reglinski
authors.affiliation: Faculty of Medicine, Imperial College London, Hammersmith Campus, Du Cane Road, London W12 0NN, United Kingdom.
authors.forename: Nicola N
authors.initials: NN
authors.lastname: Lynskey
authors.affiliation: Faculty of Medicine, Imperial College London, Hammersmith Campus, Du Cane Road, London W12 0NN, United Kingdom. Electronic address: s.sriskandan@imperial.ac.uk.
authors.forename: Shiranee
authors.initials: S
authors.lastname: Sriskandan
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D013290:Streptococcal Infections / Q000097:blood* / Q000276:immunology / Q000382:microbiology*; D013297:Streptococcus pyogenes / Q000201:enzymology / Q000254:growth & development / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; C442815:Mac-1-like protein, Streptococcus
keywords: Group A streptococcus; Lancefield assay; Streptococcus pyogenes; Surrogate of immunity