title: Mouse Models for Immune-Mediated Platelet Destruction or Immune Thrombocytopenia (ITP).
abstract: Immune thrombocytopenia (ITP) is a debilitating, life-threatening autoimmune disorder affecting more than 4 in every 100,000 adults annually, stemming from the production of antiplatelet antibody resulting in accelerated platelet destruction and thrombocytopenia. Numerous animal models of ITP have been developed that contributed to the basic understanding of the underlying mechanisms of ITP onset, progression, and maintenance. Rodent models that develop ITP spontaneously, or by passive transfer of an antiplatelet sera or antibody, play an instrumental role in the investigation of ITP mechanisms responsible for the breakdown of tolerance in human ITP, in studies of the immunopathology underlying the progression of platelet destruction, and in elucidation of the mechanisms of therapeutic amelioration of ITP by existing and new therapeutic modalities. This unit captures the protocols for the implementation and readout of passive antibody transfer mouse models of ITP, established by the infusion of a commercially-available monoclonal rat anti-mouse CD41 platelet antibody.
authors.affiliation: Centre for Innovation, Canadian Blood Services, Toronto, Ontario, Canada.
authors.forename: Anton
authors.initials: A
authors.lastname: Neschadim
authors.affiliation: Centre for Innovation, Canadian Blood Services, Toronto, Ontario, Canada.|Departments of Medicine and Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada.|Division of Advanced Diagnostics, Infection and Immunity Group, Toronto General Research Institute, Toronto, Ontario, Canada.
authors.forename: Donald R
authors.initials: DR
authors.lastname: Branch
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000276:immunology; D001323:Autoantibodies / Q000008:administration & dosage / Q000276:immunology; D015551:Autoimmunity; D004195:Disease Models, Animal; D005260:Female; D016756:Immunoglobulins, Intravenous; D051379:Mice; D010976:Platelet Count; D040201:Platelet Membrane Glycoprotein IIb / Q000008:administration & dosage / Q000276:immunology; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000097:blood* / Q000188:drug therapy / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D016756:Immunoglobulins, Intravenous; D040201:Platelet Membrane Glycoprotein IIb
keywords: ITP; IVIg; autoimmune disease: mouse model; immune thrombocytopenia; intravenous immunoglobulin; platelets