title: Whey protein hydrolysates enhance water absorption in the perfused small intestine of anesthetized rats.
abstract: We evaluated the effect of whey protein hydrolysates (WPH) on the water absorption rate in the small intestine using a rat small intestine perfusion model. The rate was significantly higher with 5 g/L WPH than with 5 g/L soy protein hydrolysates or physiological saline (p < 0.05). WPH dose-dependently increased the water absorption rate in the range of 1.25-10.0 g/L. WPH showed a significantly higher rate than an amino acid mixture whose composition was equal to that of WPH (p < 0.05). The addition of 4-aminomethylbenzoic acid, an inhibitor of PepT1, significantly suppressed WPH's enhancement of water absorption (p < 0.05). The rate of water absorption was significantly correlated with that of peptides/amino acids absorption in WPH (r = 0.82, p < 0.01). These data suggest that WPH have a high water absorption-promoting effect, to which PepT1 contributes.
authors.affiliation: a Food Science Research Laboratories, R&D Division , Meiji Co., Ltd. , Odawara , Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Ito
authors.affiliation: a Food Science Research Laboratories, R&D Division , Meiji Co., Ltd. , Odawara , Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Yamaguchi
authors.affiliation: a Food Science Research Laboratories, R&D Division , Meiji Co., Ltd. , Odawara , Japan.
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Noma
authors.affiliation: a Food Science Research Laboratories, R&D Division , Meiji Co., Ltd. , Odawara , Japan.
authors.forename: Taketo
authors.initials: T
authors.lastname: Yamaji
authors.affiliation: a Food Science Research Laboratories, R&D Division , Meiji Co., Ltd. , Odawara , Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Itoh
authors.affiliation: b College of Bioresource Sciences, Nihon University , Fujisawa , Japan.
authors.forename: Munehiro
authors.initials: M
authors.lastname: Oda
mesh_terms: D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D001692:Biological Transport; D004396:Coloring Agents / Q000378:metabolism; D015870:Gene Expression; D007408:Intestinal Absorption / Q000187:drug effects*; D007421:Intestine, Small / Q000187:drug effects* / Q000378:metabolism; D007700:Kinetics; D008297:Male; D000074011:Peptide Transporter 1; D010477:Perfusion; D010637:Phenolsulfonphthalein / Q000378:metabolism; D011492:Protein Hydrolysates / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D027981:Symporters / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D014867:Water / Q000378:metabolism*; D000067816:Whey Proteins / Q000737:chemistry*; D062366:para-Aminobenzoates / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D004396:Coloring Agents; D000074011:Peptide Transporter 1; D011492:Protein Hydrolysates; C489940:Slc15a1 protein, rat; D027981:Symporters; D000067816:Whey Proteins; D062366:para-Aminobenzoates; D014867:Water; C015668:4-aminomethylbenzoic acid; D010637:Phenolsulfonphthalein
keywords: PepT1; amino acid mixture; water absorption; whey protein hydrolysates