title: Evaluation of recombinant outer membrane protein C based indirect enzyme-linked immunoassay for the detection of Salmonella antibodies in poultry.
abstract: OBJECTIVE
  To evaluate the efficacy of recombinant outer membrane proteinC (rOmpC) based enzyme-linked immunoassay (ELISA) for the diagnosis of salmonellosis in poultry.
  
  
  METHODS
  Three antigens were prepared, and the indirect ELISA was standardized using the antigens and the antiserum raised in chicken against Omp and rOmpC. Sera were collected from a total of 255 apparently healthy field chickens and screened for the presence of Salmonella antibodies by this ELISA.
  
  
  RESULTS
  The sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of Omp revealed major polypeptides at 36, 42 and 52 kDa, and the rOmpC was evident by a single protein band of 43 kDa. The Omp and rOmpC antigen revealed an optimum concentration of 78 and 156 ng, respectively, in the assay, while the whole cell antigen gave an optimum reaction at a concentration of 10(6) organisms/ml. The test was found to be specific as it did not react with any of the antisera of seven other organisms. The developed ELISA detected Salmonella antibodies from 22 (8.62%) samples with rOmpC antigen, while 24 (9.41%) samples gave a positive reaction with both Omp and whole cell antigens.
  
  
  CONCLUSIONS
  We suggest rOmpC based indirect ELISA as a suitable screening tool for serological monitoring of poultry flocks.
authors.affiliation: Department of Veterinary Public Health and Epidemiology, College of Veterinary & Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh, India.
authors.forename: Jinu
authors.initials: J
authors.lastname: Manoj
authors.affiliation: Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India.
authors.forename: Rajesh K
authors.initials: RK
authors.lastname: Agarwal
authors.affiliation: Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India.
authors.forename: Blessa
authors.initials: B
authors.lastname: Sailo
authors.affiliation: Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India.
authors.forename: Mudasir Ahmed
authors.initials: MA
authors.lastname: Wani
authors.affiliation: Department of Livestock Production and Management, College of Veterinary & Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh, India.
authors.forename: Manoj Kumar
authors.initials: MK
authors.lastname: Singh
keywords: Salmonella; antibody; antigen; outer membrane protein; poultry