title: The Effects of IL-22 on the Inflammatory Mediator Production, Proliferation, and Barrier Function of HUVECs.
abstract: The aim of this study was to investigate the effects of interleukin (IL)-22 on proliferation function and inflammatory mediator production and barrier function of human umbilical vein endothelial cells (HUVECs). The expression of mRNA was detected by RT-PCR. The proliferation ability of cells was evaluated using a cell counting kit assay. Real-time quantitative PCR and Western blot were used to detect the expression of inflammatory mediators. The endothelial barrier permeability was assessed by measuring permeability to FITC-labeled dextran. The distribution of occludin was detected by immunofluorescence. IL-22R1 mRNA expression was noted in HUVECs. IL-22 could enhance the proliferation ability of HUVECs and suppress lipopolysaccharide (LPS)-induced proliferation inhibition in these cells. IL-22 also enhanced the production of CCL2 and CCL20 by HUVECs. Besides, IL-22 could improve barrier function and decrease LPS-induced increased cellular permeability and inhibit the LPS-induced destruction of occludin in HUVECs. IL-22 may play a protective role in the development of vasculitis.
authors.affiliation: The First Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Xian
authors.initials: X
authors.lastname: He
authors.affiliation: The First Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: The First Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: The First Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Aijun
authors.initials: A
authors.lastname: Chen
authors.affiliation: The First Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Kui
authors.initials: K
authors.lastname: Shan
authors.affiliation: The First Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Jin
authors.initials: J
authors.lastname: Chen
authors.affiliation: The First Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Hengguang
authors.initials: H
authors.lastname: Zhao
authors.affiliation: The First Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Xiaojiao
authors.initials: X
authors.lastname: Zhang
authors.affiliation: The First Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China. taocaicq1@sina.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Cai
mesh_terms: D018916:Blood-Aqueous Barrier / Q000187:drug effects*; D002199:Capillary Permeability / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D018836:Inflammation Mediators / Q000032:analysis*; D007378:Interleukins / Q000235:genetics / Q000494:pharmacology*; D062793:Occludin / Q000187:drug effects; D000097043:Interleukin-22
chemical_list: D018836:Inflammation Mediators; D007378:Interleukins; D062793:Occludin; D000097043:Interleukin-22
keywords: Behcet syndrome; IL-22; LPS; inflammatory; vasculitis