title: CKAP2 is necessary to ensure the faithful spindle bipolarity in a dividing diploid hepatocyte.
abstract: Spindle bipolarity is crucial for segregating chromosome during somatic cell division. Previous studies have suggested that cytoskeleton associated protein 2 (CKAP2) is involved in spindle assembly and chromosome segregation. In this study, we show that CKAP2-depleted primary hepatocytes exhibit over-duplicated centrosomes with disjoined chromosomes from metaphase plate. These cells proceed to apoptosis or multipolar cell division and subsequent apoptotic cell death. In addition, a mouse liver regeneration experiment showed a marked decrease in efficiency of hepatic regeneration in CKAP2-depleted liver. These data suggest a physiological role of CKAP2 in the formation of spindle bipolarity, which is necessary for maintaining chromosomal stability.
authors.affiliation: Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea. Electronic address: vdarios@naver.com.
authors.forename: Bum Ho
authors.initials: BH
authors.lastname: Yoo
authors.affiliation: Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea. Electronic address: chihupark@naver.com.
authors.forename: Chi-Hu
authors.initials: CH
authors.lastname: Park
authors.affiliation: Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea. Electronic address: juny81@hanmail.net.
authors.forename: Hyun-Jun
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: School of Life Sciences, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea. Electronic address: delphinus.med@gmail.com.
authors.forename: Du-Seock
authors.initials: DS
authors.lastname: Kang
authors.affiliation: Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea. Electronic address: cdbae@skku.edu.
authors.forename: Chang-Dae
authors.initials: CD
authors.lastname: Bae
mesh_terms: D000818:Animals; D002455:Cell Division / Q000235:genetics*; D002478:Cells, Cultured; D018385:Centrosome / Q000502:physiology; D020090:Chromosome Segregation / Q000235:genetics; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D004171:Diploidy; D022781:Hepatocytes / Q000166:cytology*; D059566:M Phase Cell Cycle Checkpoints / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008941:Spindle Apparatus / Q000235:genetics*
chemical_list: C494575:CKAP2 protein, mouse; D003598:Cytoskeletal Proteins
keywords: CKAP2; Centrosome; Chromosome segregation; Multipolar cell division