title: Endoplasmic reticulum stress-induced apoptotic pathway and mitochondrial dysregulation in HeLa cells treated with dichloromethane extract of Dillenia suffruticosa.
abstract: Ethyl acetate and dichloromethane extract of Dillenia suffruticosa (EADS and DCMDS, respectively) can be a potential anticancer agent. The effects of EADS and DCMDS on the growth of HeLa cervical cancer cells and the expression of apoptotic-related proteins had been investigated in vitro. Cytotoxicity of the extracts toward the cells was determined by 5-diphenyltetrazolium bromide assay, the effects on cell cycle progression and the mode of cell death were analyzed by flow cytometry technique, while the effects on apoptotic-related genes and proteins were evaluated by quantitative real-time polymerase chain reaction, and Western blot and enzyme-linked immunosorbent assay, respectively. Treatment with DCMDS inhibited (P < 0.05) proliferation and induced apoptosis in HeLa cells. The expression of cyclin B1 was downregulated that led to G2/M arrest in the cells after treatment with DCMDA. In summary, DCMDS induced apoptosis in HeLa cells via endoplasmic reticulum stress-induced apoptotic pathway and dysregulation of mitochondria. The data suggest the potential application of DCMDS in the treatment of cervical cancer.
authors.affiliation: Department of Biomedical Sciences, Faculty of Medicine and Health Science, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; College of Medical Laboratory Technology, Institute for Medical Research, Jin Pahang, 50588 Kuala Lumpur, Malaysia.
authors.forename: Wan Abd Ghani
authors.initials: WA
authors.lastname: Wan Nor Hafiza
authors.affiliation: Department of Biomedical Sciences, Faculty of Medicine and Health Science, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Laboratory of Molecular Biomedicine, Institute of Bioscience, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Latifah Saiful
authors.initials: LS
authors.lastname: Yazan
authors.affiliation: Laboratory of Molecular Biomedicine, Institute of Bioscience, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Yin Sim
authors.initials: YS
authors.lastname: Tor
authors.affiliation: Laboratory of Molecular Biomedicine, Institute of Bioscience, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Jhi Biau
authors.initials: JB
authors.lastname: Foo
authors.affiliation: Department of Biomedical Sciences, Faculty of Medicine and Health Science, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Laboratory of Molecular Biomedicine, Institute of Bioscience, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Nurdin
authors.initials: N
authors.lastname: Armania
authors.affiliation: Department of Microbiology and Pathology, Faculty of Veterinary Medicine, 43400 UPM Serdang, Selangor, Malaysia; UPM-MAKNA Cancer Research Laboratory, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Heshu Sulaiman
authors.initials: HS
authors.lastname: Rahman
keywords: Apoptosis; Dillenia suffruticosa; cell cycle arrest; cervical cancer; endoplasmic reticulum stress