title: Protective Capacity of Resveratrol, a Natural Polyphenolic Compound, against Deoxynivalenol-Induced Intestinal Barrier Dysfunction and Bacterial Translocation.
abstract: Contamination of food/feedstuffs by mycotoxins is a serious problem worldwide, causing severe economic losses and serious health problems in animals/humans. Deoxynivalenol (DON) is a major mycotoxin contaminant and is known to impair intestinal barrier function. Grapes and red wine are rich in polyphenols, such as resveratrol (RES), which has striking antioxidant and anti-inflammatory activities. RES is a food-derived component; therefore, it may be simultaneously present with DON in the gastrointestinal tract. The aim of this study was to explore in vitro protective effects of RES against DON-induced intestinal damage. The results showed that RES could protect DON-induced bacteria translocation because of enhanced of intestinal barrier function by restoring the DON-induced decrease in transepithelial electrical resistance and increase in paracellular permeability. Further mechanistic studies demonstrated that RES protects against DON-induced barrier dysfunction by promoting the assembly of claudin-4 in the tight junction complex. This is probably mediated through modulation of IL-6 and IL-8 secretion via mitogen-activated protein kinase-dependent pathways. Our results imply that RES can protect against DON-induced intestinal damage and that RES may be used as a novel dietary intervention strategy to reduce DON toxicity in animals/humans.
authors.affiliation: School of Biological Sciences, Faculty of Science, The University of Hong Kong , Kadoorie Biological Sciences Building, Pokfulam, Hong Kong.
authors.forename: Ka-Ho
authors.initials: KH
authors.lastname: Ling
authors.affiliation: School of Biological Sciences, Faculty of Science, The University of Hong Kong , Kadoorie Biological Sciences Building, Pokfulam, Hong Kong.
authors.forename: Murphy Lam Yim
authors.initials: ML
authors.lastname: Wan
authors.affiliation: School of Biological Sciences, Faculty of Science, The University of Hong Kong , Kadoorie Biological Sciences Building, Pokfulam, Hong Kong.|Institute of Public Health and Clinical Nutrition, University of Eastern Finland , FI-70211 Kuopio, Finland.
authors.forename: Hani
authors.initials: H
authors.lastname: El-Nezami
authors.affiliation: School of Biological Sciences, Faculty of Science, The University of Hong Kong , Kadoorie Biological Sciences Building, Pokfulam, Hong Kong.
authors.forename: Mingfu
authors.initials: M
authors.lastname: Wang
mesh_terms: D000818:Animals; D018988:Bacterial Translocation / Q000187:drug effects*; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects* / Q000503:physiopathology; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013552:Swine; D014255:Trichothecenes / Q000633:toxicity*
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D013267:Stilbenes; D014255:Trichothecenes; C007262:deoxynivalenol; D000077185:Resveratrol