title: Conformational photoswitching of a synthetic peptide foldamer bound within a phospholipid bilayer.
abstract: The dynamic properties of foldamers, synthetic molecules that mimic folded biomolecules, have mainly been explored in free solution. We report on the design, synthesis, and conformational behavior of photoresponsive foldamers bound in a phospholipid bilayer akin to a biological membrane phase. These molecules contain a chromophore, which can be switched between two configurations by different wavelengths of light, attached to a helical synthetic peptide that both promotes membrane insertion and communicates conformational change along its length. Light-induced structural changes in the chromophore are translated into global conformational changes, which are detected by monitoring the solid-state (19)F nuclear magnetic resonance signals of a remote fluorine-containing residue located 1 to 2 nanometers away. The behavior of the foldamers in the membrane phase is similar to that of analogous compounds in organic solvents.
authors.affiliation: School of Chemistry, University of Manchester, Manchester M13 9PL, UK.
authors.forename: Matteo
authors.initials: M
authors.lastname: De Poli
authors.affiliation: School of Chemistry, University of Manchester, Manchester M13 9PL, UK.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Zawodny
authors.affiliation: School of Chemistry, University of Manchester, Manchester M13 9PL, UK.
authors.forename: Ophélie
authors.initials: O
authors.lastname: Quinonero
authors.affiliation: Department of Chemistry, University of Hull, Hull HU6 7RX, UK.
authors.forename: Mark
authors.initials: M
authors.lastname: Lorch
authors.affiliation: School of Chemistry, University of Manchester, Manchester M13 9PL, UK. Manchester Institute of Biotechnology, University of Manchester, Manchester M1 7DN, UK.
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Webb
authors.affiliation: School of Chemistry, University of Bristol, Bristol BS8 1TS, UK. j.clayden@bristol.ac.uk.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Clayden
mesh_terms: D008027:Light; D008051:Lipid Bilayers / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D010455:Peptides / Q000737:chemistry* / Q000528:radiation effects; D010713:Phosphatidylcholines / Q000737:chemistry* / Q000528:radiation effects; D010743:Phospholipids / Q000737:chemistry* / Q000528:radiation effects; D055668:Photochemical Processes; D011487:Protein Conformation; D017510:Protein Folding
chemical_list: D008051:Lipid Bilayers; D010455:Peptides; D010713:Phosphatidylcholines; D010743:Phospholipids; C017251:1,2-oleoylphosphatidylcholine