title: Tuning cell adhesion on polymeric and nanocomposite surfaces: Role of topography versus superhydrophobicity.
abstract: Development of surface modification procedures which allow tuning the cell adhesion on the surface of biomaterials and devices is of great importance. In this study, the effects of different topographies and wettabilities on cell adhesion behavior of polymeric surfaces are investigated. To this end, an improved phase separation method was proposed to impart various wettabilities (hydrophobic and superhydrophobic) on polypropylene surfaces. Surface morphologies and compositions were characterized by scanning electron microscopy and X-ray photoelectron spectroscopy, respectively. Cell culture was conducted to evaluate the adhesion of 4T1 mouse mammary tumor cells. It was found that processing conditions such as drying temperature is highly influential in cell adhesion behavior due to the formation of an utterly different surface topography. It was concluded that surface topography plays a more significant role in cell adhesion behavior rather than superhydrophobicity since the nano-scale topography highly inhibited the cell adhesion as compared to the micro-scale topography. Such cell repellent behavior could be very useful in many biomedical devices such as those in drug delivery and blood contacting applications as well as biosensors.
authors.affiliation: Department of Chemical Engineering, Shahrood Branch, Islamic Azad University, P.O. Box 36155-163, Shahrood, Iran.
authors.forename: Sepideh
authors.initials: S
authors.lastname: Zangi
authors.affiliation: Department of Polymer Engineering & Color Technology, Amirkabir University of Technology, Tehran, Iran.
authors.forename: Iman
authors.initials: I
authors.lastname: Hejazi
authors.affiliation: Department of Chemical Engineering, Shahrood Branch, Islamic Azad University, P.O. Box 36155-163, Shahrood, Iran. Electronic address: Jseyfi@gmail.com.
authors.forename: Javad
authors.initials: J
authors.lastname: Seyfi
authors.affiliation: Department of Clinical Nutrition and Dietetics, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Ehsan
authors.initials: E
authors.lastname: Hejazi
authors.affiliation: Department of Polymer Engineering, Faculty of Engineering, South Tehran Branch, Islamic Azad University, P.O. Box 19585-466, Tehran, Iran.
authors.forename: Hossein Ali
authors.initials: HA
authors.lastname: Khonakdar
authors.affiliation: School of Chemical Engineering, University of Tehran, P.O. Box 11155-4563, Tehran, Iran.
authors.forename: Seyed Mohammad
authors.initials: SM
authors.lastname: Davachi
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D051379:Mice; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D011108:Polymers / Q000737:chemistry*; D011126:Polypropylenes / Q000737:chemistry; D013499:Surface Properties; D013696:Temperature; D017155:Wettability
chemical_list: D001672:Biocompatible Materials; D011108:Polymers; D011126:Polypropylenes
keywords: Cell adhesion; Phase separation; Polypropylene; Superhydrophobic; Topography