title: Expression of Castor LPAT2 Enhances Ricinoleic Acid Content at the sn-2 Position of Triacylglycerols in Lesquerella Seed.
abstract: Lesquerella is a potential industrial oilseed crop that makes hydroxy fatty acid (HFA). Unlike castor its seeds are not poisonous but accumulate lesquerolic acid mostly at the sn-1 and sn-3 positions of triacylglycerol (TAG), whereas castor contains ricinoleic acid (18:1OH) at all three positions. To investigate whether lesquerella can be engineered to accumulate HFAs in the sn-2 position, multiple transgenic lines were made that express castor lysophosphatidic acid acyltransferase 2 (RcLPAT2) in the seed. RcLPAT2 increased 18:1OH at the sn-2 position of TAGs from 2% to 14%-17%, which resulted in an increase of tri-HFA-TAGs from 5% to 13%-14%. Our result is the first example of using a LPAT to increase ricinoleic acid at the sn-2 position of seed TAG. This work provides insights to the mechanism of HFA-containing TAG assembly in lesquerella and directs future research to optimize this plant for HFA production.
authors.affiliation: Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 800 Buchanan St., Albany, CA 94710, USA. grace.chen@ars.usda.gov.
authors.forename: Grace Q
authors.initials: GQ
authors.lastname: Chen
authors.affiliation: Institute of Biological Chemistry, Washington State University, Pullman, Washington, DC 99164, USA. harrie.van-erp@rothamsted.ac.uk.|Department of Plant Biology and Crop Science, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK. harrie.van-erp@rothamsted.ac.uk.
authors.forename: Harrie
authors.initials: H
authors.lastname: van Erp
authors.affiliation: Department of Plant Biology and Crop Science, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK. jmartinm83@gmail.com.
authors.forename: Jose
authors.initials: J
authors.lastname: Martin-Moreno
authors.affiliation: Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 800 Buchanan St., Albany, CA 94710, USA. kumiko.johnson@ars.usda.gov.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Johnson
authors.affiliation: Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 800 Buchanan St., Albany, CA 94710, USA. evadesalta@gmail.com.
authors.forename: Eva
authors.initials: E
authors.lastname: Morales
authors.affiliation: Institute of Biological Chemistry, Washington State University, Pullman, Washington, DC 99164, USA. jab@wsu.edu.
authors.forename: John
authors.initials: J
authors.lastname: Browse
authors.affiliation: Department of Plant Biology and Crop Science, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK. peter.eastmond@rothamsted.ac.uk.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Eastmond
authors.affiliation: Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 800 Buchanan St., Albany, CA 94710, USA. jiann.lin@ars.usda.gov.
authors.forename: Jiann-Tsyh
authors.initials: JT
authors.lastname: Lin
mesh_terms: D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D019607:Brassicaceae / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005227:Fatty Acids / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012277:Ricinoleic Acids / Q000032:analysis / Q000378:metabolism*; D012278:Ricinus / Q000235:genetics; D012639:Seeds / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014280:Triglycerides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D005227:Fatty Acids; D010940:Plant Proteins; D012277:Ricinoleic Acids; D014280:Triglycerides; D000217:Acyltransferases; C018764:2-acylglycerophosphate acyltransferase; C030521:ricinoleic acid
keywords: Physaria fendleri; hydroxy fatty acid; lesquerella; lysophosphatidic acid acyltransferase; metabolic engineering; seed triacylglycerol