title: Identification of Serum Peptidome Signatures of Non-Small Cell Lung Cancer.
abstract: Due to high mortality rates of lung cancer, there is a need for identification of new, clinically useful markers, which improve detection of this tumor in early stage of disease. In the current study, serum peptide profiling was evaluated as a diagnostic tool for non-small cell lung cancer patients. The combination of the ZipTip technology with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) for the analysis of peptide pattern of cancer patients (n = 153) and control subjects (n = 63) was presented for the first time. Based on the observed significant differences between cancer patients and control subjects, the classification model was created, which allowed for accurate group discrimination. The model turned out to be robust enough to discriminate a new validation set of samples with satisfactory sensitivity and specificity. Two peptides from the diagnostic pattern for non-small cell lung cancer (NSCLC) were identified as fragments of C3 and fibrinogen α chain. Since ELISA test did not confirm significant differences in the expression of complement component C3, further study will involve a quantitative approach to prove clinical utility of the other proteins from the proposed multi-peptide cancer signature.
authors.affiliation: Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, Grunwaldzka 6 Street, 60-780 Poznan, Poland. a.klupczynska@gmail.com.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Klupczynska
authors.affiliation: Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, Grunwaldzka 6 Street, 60-780 Poznan, Poland. agata_swiatly@wp.pl.
authors.forename: Agata
authors.initials: A
authors.lastname: Swiatly
authors.affiliation: Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, Grunwaldzka 6 Street, 60-780 Poznan, Poland. jo.hajduk@gmail.com.
authors.forename: Joanna
authors.initials: J
authors.lastname: Hajduk
authors.affiliation: Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, Grunwaldzka 6 Street, 60-780 Poznan, Poland. jmatysiak@ump.edu.pl.
authors.forename: Jan
authors.initials: J
authors.lastname: Matysiak
authors.affiliation: Department of Thoracic Surgery, Poznan University of Medical Sciences, Szamarzewskiego 62 Street, 60-569 Poznan, Poland. dyszkiewicz@wp.pl.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Dyszkiewicz
authors.affiliation: Department of Thoracic Surgery, Poznan University of Medical Sciences, Szamarzewskiego 62 Street, 60-569 Poznan, Poland. krystianp@hotmail.com.
authors.forename: Krystian
authors.initials: K
authors.lastname: Pawlak
authors.affiliation: Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, Grunwaldzka 6 Street, 60-780 Poznan, Poland. zkokot@ump.edu.pl.
authors.forename: Zenon J
authors.initials: ZJ
authors.lastname: Kokot
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D019540:Area Under Curve; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism / Q000473:pathology*; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D003176:Complement C3 / Q000032:analysis / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005340:Fibrinogen / Q000032:analysis; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000032:analysis* / Q000302:isolation & purification; D012372:ROC Curve; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D003176:Complement C3; D010455:Peptides; C464794:fibrinogen Aalpha; D005340:Fibrinogen
keywords: MALDI-TOF-MS; ZipTip enrichment; non-small cell lung cancer; peptide profiling