title: Transformation of Schizosaccharomyces pombe: Electroporation Procedure.
abstract: Transformation ofSchizosaccharomyces pombewith DNA requires the conditioning of cells to promote DNA uptake followed by cell growth under conditions that select and maintain the plasmid or integration event. The three main methodologies are electroporation, treatment with lithium cations, and transformation of protoplasts. This protocol describes transformation by electroporation. It involves pretreatingS. pombecells with dithiothreitol (DTT), which increases the transformation efficiency once the electric pulse is applied.
authors.affiliation: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Falmer, Brighton, E. Sussex BN1 9RQ, United Kingdom.
authors.forename: Johanne M
authors.initials: JM
authors.lastname: Murray
authors.affiliation: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Falmer, Brighton, E. Sussex BN1 9RQ, United Kingdom.
authors.forename: Adam T
authors.initials: AT
authors.lastname: Watson
authors.affiliation: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Falmer, Brighton, E. Sussex BN1 9RQ, United Kingdom.
authors.forename: Antony M
authors.initials: AM
authors.lastname: Carr
mesh_terms: D004229:Dithiothreitol / Q000378:metabolism; D018274:Electroporation / Q000379:methods*; D012568:Schizosaccharomyces / Q000187:drug effects / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D004229:Dithiothreitol