title: Allicin relaxes isolated mesenteric arteries through activation of PKA-KATPchannel in rat.
abstract: Allicin is a natural effective organosulfur compound isolated from garlic, which possesses many beneficial properties, such as antibacterial, anti-inflammatory, antimicrobial, hypotensive and hypolipidemic. In the present study, we investigated the effects and the underlying mechanisms of allicin on isolated mesenteric arteries (MAs). We examined MAs relaxation induced by allicin on rat-isolated mesenteric artery (MA) rings, the KATPchannels with patch, and the expression of Kir6.1 and SUR2B with western blotting and NO production with Diaminofluorescein-FM diacetate (DAF-FMDA) in rat mesenteric artery smooth muscle cells (MASMCs). The results showed that allicin elicited the dose-dependent vasorelaxation effect with phenylephrine (PE) precontracted rat MA rings. The vasorelaxation effect was endothelium and NO independent but could be diminished by inhibition of PKA and KATPchannels in the vascular smooth muscle. Allicin activated KATPchannels in rat MASMCs, and the activation of KATPchannels was inhibited by the inhibitors of PKA and KATPchannels. But allicin had no effect on the expression of KATPsubtypes Kir6.1 and SUR2B. These observations suggest that allicin exerts vasorelaxation effect through activation of PKA-KATP-signaling pathway.
authors.affiliation: a Department of Biopharmaceutical Sciences , College of Pharmacy, Harbin Medical University , PR China.
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Li
authors.affiliation: b Department of Biopharmaceutical Sciences , College of Pharmacy, Harbin Medical University-Daqing , PR China , and.
authors.forename: Shuo
authors.initials: S
authors.lastname: Chen
authors.affiliation: c College of Food Science, Northeast Agricultural University , PR China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Yang
authors.affiliation: a Department of Biopharmaceutical Sciences , College of Pharmacy, Harbin Medical University , PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Liao
authors.affiliation: a Department of Biopharmaceutical Sciences , College of Pharmacy, Harbin Medical University , PR China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Li
authors.affiliation: a Department of Biopharmaceutical Sciences , College of Pharmacy, Harbin Medical University , PR China.
authors.forename: Jiali
authors.initials: J
authors.lastname: Li
authors.affiliation: a Department of Biopharmaceutical Sciences , College of Pharmacy, Harbin Medical University , PR China.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: c College of Food Science, Northeast Agricultural University , PR China.
authors.forename: Daling
authors.initials: D
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D015153:Blotting, Western; D002478:Cells, Cultured; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D004195:Disease Models, Animal; D004220:Disulfides; D004594:Electrophysiology; D004789:Enzyme Activation; D054086:KATP Channels / Q000378:metabolism; D008297:Male; D008638:Mesenteric Arteries / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects*; D013441:Sulfinic Acids / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D004220:Disulfides; D054086:KATP Channels; D013441:Sulfinic Acids; C006452:allicin; D017868:Cyclic AMP-Dependent Protein Kinases
keywords: Allicin; PKA–KATP-signaling pathway; mesenteric artery; vasorelaxation