title: Interlaboratory Trial for Measurement of Vitamin D and 25-Hydroxyvitamin D [25(OH)D] in Foods and a Dietary Supplement Using Liquid Chromatography-Mass Spectrometry.
abstract: Assessment of total vitamin D intake from foods and dietary supplements (DSs) may be incomplete if 25-hydroxyvitamin D [25(OH)D] intake is not included. However, 25(OH)D data for such intake assessments are lacking, no food or DS reference materials (RMs) are available, and comparison of laboratory performance has been needed. The primary goal of this study was to evaluate whether vitamin D3 and 25(OH)D3 concentrations in food and DS materials could be measured with acceptable reproducibility. Five experienced laboratories from the United States and other countries participated, all using liquid chromatography tandem-mass spectrometry but no common analytical protocol; however, various methods were used for determining vitamin D3 in the DS. Five animal-based materials (including three commercially available RMs) and one DS were analyzed. Reproducibility results for the materials were acceptable. Thus, it is possible to obtain consistent results among experienced laboratories for vitamin D3 and 25(OH)D3 in foods and a DS.
authors.affiliation: Nutrient Data Laboratory, Agricultural Research Service, U.S. Department of Agriculture , 10300 Baltimore Avenue, Building 005, BARC-West, Beltsville, Maryland 20705, United States.
authors.forename: Janet Maxwell
authors.initials: JM
authors.lastname: Roseland
authors.affiliation: Nutrient Data Laboratory, Agricultural Research Service, U.S. Department of Agriculture , 10300 Baltimore Avenue, Building 005, BARC-West, Beltsville, Maryland 20705, United States.
authors.forename: Kristine Y
authors.initials: KY
authors.lastname: Patterson
authors.affiliation: Nutrient Data Laboratory, Agricultural Research Service, U.S. Department of Agriculture , 10300 Baltimore Avenue, Building 005, BARC-West, Beltsville, Maryland 20705, United States.
authors.forename: Karen W
authors.initials: KW
authors.lastname: Andrews
authors.affiliation: Biochemistry Department (0308), Virginia Tech , 304 Engel Hall, Blacksburg, Virginia 24061, United States.
authors.forename: Katherine M
authors.initials: KM
authors.lastname: Phillips
authors.affiliation: Chemical Sciences Division, National Institute of Standards and Technology , MS 8392, 100 Bureau Drive, Gaithersburg, Maryland 20899-8392, United States.
authors.forename: Melissa M
authors.initials: MM
authors.lastname: Phillips
authors.affiliation: Nutrient Data Laboratory, Agricultural Research Service, U.S. Department of Agriculture , 10300 Baltimore Avenue, Building 005, BARC-West, Beltsville, Maryland 20705, United States.
authors.forename: Pamela R
authors.initials: PR
authors.lastname: Pehrsson
authors.affiliation: Food and Nutrition Laboratory, Health Canada , 1001 St-Laurent Ouest, Longueuil, Québec, Canada J4K IC7.
authors.forename: Guy L
authors.initials: GL
authors.lastname: Dufresne
authors.affiliation: Division of Food Chemistry, National Food Institute, Technical University of Denmark , Søborg, Denmark.
authors.forename: Jette
authors.initials: J
authors.lastname: Jakobsen
authors.affiliation: Nutrient Data Laboratory, Agricultural Research Service, U.S. Department of Agriculture , 10300 Baltimore Avenue, Building 005, BARC-West, Beltsville, Maryland 20705, United States.
authors.forename: Pavel A
authors.initials: PA
authors.lastname: Gusev
authors.affiliation: Nutrient Data Laboratory, Agricultural Research Service, U.S. Department of Agriculture , 10300 Baltimore Avenue, Building 005, BARC-West, Beltsville, Maryland 20705, United States.
authors.forename: Sushma
authors.initials: S
authors.lastname: Savarala
authors.affiliation: Nutrient Data Laboratory, Agricultural Research Service, U.S. Department of Agriculture , 10300 Baltimore Avenue, Building 005, BARC-West, Beltsville, Maryland 20705, United States.
authors.forename: Quynhanh V
authors.initials: QV
authors.lastname: Nguyen
authors.affiliation: Heartland Assays, LLC , Suite 4400, 2711 South Loop Drive, Ames, Iowa 50010, United States.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Makowski
authors.affiliation: Covance Laboratories , 3301 Kinsman Boulevard, Madison, Wisconsin 53704, United States.
authors.forename: Chad R
authors.initials: CR
authors.lastname: Scheuerell
authors.affiliation: Food and Nutrition Laboratory, Health Canada , 1001 St-Laurent Ouest, Longueuil, Québec, Canada J4K IC7.
authors.forename: Guillaume P
authors.initials: GP
authors.lastname: Larouche
authors.affiliation: Chemical Sciences Division, National Institute of Standards and Technology , MS 8392, 100 Bureau Drive, Gaithersburg, Maryland 20899-8392, United States.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Wise
authors.affiliation: Food Composition and Method Development Laboratory, Agricultural Research Service, U.S. Department of Agriculture , Building 161, BARC-East, 10300 Baltimore Avenue, Beltsville, Maryland 20705, United States.
authors.forename: James M
authors.initials: JM
authors.lastname: Harnly
authors.affiliation: Nutrient Data Laboratory, Agricultural Research Service, U.S. Department of Agriculture , 10300 Baltimore Avenue, Building 005, BARC-West, Beltsville, Maryland 20705, United States.
authors.forename: Juhi R
authors.initials: JR
authors.lastname: Williams
authors.affiliation: Office of Dietary Supplements, National Institutes of Health , 3B01, MSC 7517, 6100 Executive Boulevard, Bethesda, Maryland 20892, United States.
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Betz
authors.affiliation: Office of Dietary Supplements, National Institutes of Health , 3B01, MSC 7517, 6100 Executive Boulevard, Bethesda, Maryland 20892, United States.
authors.forename: Christine L
authors.initials: CL
authors.lastname: Taylor
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D019587:Dietary Supplements / Q000032:analysis*; D005504:Food Analysis; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D014807:Vitamin D; C104450:25-hydroxyvitamin D
keywords: 25-hydroxyvitamin D3 (25-hydroxycholcalciferol); dietary supplement; food; reference material; vitamin D3 (cholecalciferol)