title: Evaluation of Galactose Adapted Yeasts for Bioethanol Fermentation from Kappaphycus alvarezii Hydrolyzates.
abstract: Bioethanol was produced from Kappaphycus alvarezii seaweed biomass using separate hydrolysis and fermentation (SHF). Pretreatment was evaluated for 60 min at 121°C using 12% (w/v) biomass slurry with 364 mM H2SO4. Enzymatic saccharification was then carried out at 45°C for 48 h using Celluclast 1.5 L. Ethanol fermentation with 12% (w/v) K. alvarezii hydrolyzate was performed using the yeasts Saccharomyces cerevisiae KCTC1126, Kluyveromyces marxianus KCTC7150, and Candida lusitaniae ATCC42720 with or without prior adaptation to high concentrations of galactose. When non-adapted S. cerevisiae, K. marxianus, and C. lusitaniae were used, 11.5 g/l, 6.7 g/l, and 6.0 g/l of ethanol were produced, respectively. When adapted S. cerevisiae, K. marxianus, and C. lusitaniae were used, 15.8 g/l, 11.6 g/l, and 13.4 g/l of ethanol were obtained, respectively. The highest ethanol concentration was 15.8 g/l, with YEtOH = 0.43 and YT% = 84.3%, which was obtained using adapted S. cerevisiae.
authors.affiliation: Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: Trung Hau
authors.initials: TH
authors.lastname: Nguyen
authors.affiliation: Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: Chae Hun
authors.initials: CH
authors.lastname: Ra
authors.affiliation: Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: In Yung
authors.initials: IY
authors.lastname: Sunwoo
authors.affiliation: Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: Gwi-Taek
authors.initials: GT
authors.lastname: Jeong
authors.affiliation: Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: Sung-Koo
authors.initials: SK
authors.lastname: Kim
mesh_terms: D000220:Adaptation, Biological; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005690:Galactose / Q000378:metabolism*; D006868:Hydrolysis; D000461:Rhodophyta / Q000378:metabolism*; D015003:Yeasts / Q000378:metabolism*
chemical_list: D000431:Ethanol; D005690:Galactose
keywords: Kappaphycus alvarezii; adaptation; enzymatic saccharification; fermentation; thermal acid hydrolysis pretreatment