title: Influence of continuous intervention on growth and metastasis of human cervical cancer cells and expression of RNAmiR-574-5p.
abstract: This study was carried out to acquire solid evidence that some common treatments could affect micro ribonucleic acids (miRNAs) by revealing the regulatory effect of genes, so as to provide a reference for further exploration of the prevention and treatment of cervical cancer. Nude mouse tumorigenicity assay was used to study the effect of inhibiting miR-574-5p on development and tumorigenic ability of Henrietta Lacks (HeLa) tumor. Cell wound scratch assay, flow cytometry and real-time quantitative polymerase chain reaction (RT-qPCR) were adopted to study the effects of anoxia and temperature, etc., on expression of miR-574-5p and QKI in HeLa as well as on the clone and migration ability of cells, to provide prevention and treatment of cervical cancer with new ideas and evidence. The results demonstrated that cervical cancer tissues had a significantly increased miR-574-5p expression compared with para-carcinoma tissues; conversely, Gomafu, overall QKI (pan-QKI) and QKI-5 messenger ribonucleic acid (mRNA) and protein expression all decreased. Part of the common nursing methods had a certain influence on miR-574-5p expression, HeLa reproduction and metastasis, and even cell cycle. For example, ultraviolet (UV) irradiation was effective in decreasing miR-574-5p expression of HeLa and inhibiting cell migration; severe hypoxia significantly decreased the survival rate of HeLa, leading to the increase of programmed death percentage and cell ratio in G2/M phase as well as the decrease of cell ratio in G1 phase. Incubation at different temperatures also affected miR-574-5p expression and cell proliferation. Thus, it can be known that miR-574-5p, Gomafu and QKI expression in cervical cancer tissues and para-carcinoma tissues are significantly up-regulated or down-regulated. Some treatments, such as UV irradiation, hypoxia, incubation temperatures, etc., can affect miR-574-5p expression and HeLa proliferation as well as metastases in different degrees. These findings provide a reference and basis for further study.
authors.affiliation: Department of Oncology, Yishui Central Hospital of Linyi, Linyi, China.
authors.forename: D L
authors.initials: DL
authors.lastname: Ma
authors.affiliation: Department of Oncology, Yishui Central Hospital of Linyi, Linyi, China.
authors.forename: J Y
authors.initials: JY
authors.lastname: Li
authors.affiliation: Department of Infectious Disease, Yishui Central Hospital of Linyi, Linyi, China.
authors.forename: Y E
authors.initials: YE
authors.lastname: Liu
authors.affiliation: Department of Oncology, Binzhou Medical College Affiliated Hospital, Binzhou, Shandong, China.
authors.forename: C M
authors.initials: CM
authors.lastname: Liu
authors.affiliation: Department of Clinical support, Dongming Peoples Hospital, Shandong, China.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Health Materials Management, Dongming Peoples Hospital, Shandong, China.
authors.forename: G Z
authors.initials: GZ
authors.lastname: Lin
authors.affiliation: Medical Social Work section, Dongming Peoples Hospital, Shandong, China.
authors.forename: J
authors.initials: J
authors.lastname: Yan
mesh_terms: D015687:Cell Hypoxia / Q000235:genetics / Q000528:radiation effects; D002465:Cell Movement / Q000235:genetics / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D002999:Clone Cells; D003080:Cold Temperature; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects; D006367:HeLa Cells; D006358:Hot Temperature; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014466:Ultraviolet Rays; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology*
chemical_list: C558811:MIRN574 microRNA, human; D035683:MicroRNAs; D009363:Neoplasm Proteins; D012333:RNA, Messenger