title: Production and assay of cellulolytic enzyme activity of Enterobacter cloacae WPL 214 isolated from bovine rumen fluid waste of Surabaya abbatoir, Indonesia.
abstract: OBJECTIVE
  This study aims to produce and assay cellulolytic enzyme activity (endo-(1,4)-β-D-glucanase, exo-(1,4)-β-D-glucanase, and β-glucosidase, at optimum temperature and optimum pH) of Enterobacter cloacae WPL 214 isolated from bovine rumen fluid waste of Surabaya Abbatoir, Indonesia.
  
  
  METHODS
  To produce enzyme from a single colony of E. cloacae WPL 214, 98 × 10(10) CFU/ml of isolates was put into 20 ml of liquid medium and incubated in a shaker incubator for 16 h at 35°C in accordance with growth time and optimum temperature of E. cloacae WPL 214. Further on, culture was centrifuged at 6000 rpm at 4°C for 15 min. Pellet was discarded while supernatant containing cellulose enzyme activity was withdrawn to assay endo-(1,4)-β-D-glucanase, exo-(1,4)-β-D-glucanase, and β-glucosidase.
  
  
  RESULTS
  Cellulase enzyme of E. cloacae WPL 214 isolates had endoglucanase activity of 0.09 U/ml, exoglucanase of 0.13 U/ml, and cellobiase of 0.10 U/ml at optimum temperature 35°C and optimum pH 5.
  
  
  CONCLUSIONS
  E. cloacae WPL 214 isolated from bovine rumen fluid waste produced cellulose enzyme with activity as cellulolytic enzyme of endo-(1,4)-β-D-glucanase, exo-(1,4)-β-D-glucanase and β-glucosidase.
authors.affiliation: Department of Animal Husbandry, Faculty of Veterinary Medicine, Airlangga University, Jl. Mulyorejo, Campus C Unair, Surabaya, Indonesia.
authors.forename: W P
authors.initials: WP
authors.lastname: Lokapirnasari
authors.affiliation: Department of Animal Husbandry, Faculty of Veterinary Medicine, Airlangga University, Jl. Mulyorejo, Campus C Unair, Surabaya, Indonesia.
authors.forename: D S
authors.initials: DS
authors.lastname: Nazar
authors.affiliation: Department of Animal Husbandry, Faculty of Veterinary Medicine, Airlangga University, Jl. Mulyorejo, Campus C Unair, Surabaya, Indonesia.
authors.forename: T
authors.initials: T
authors.lastname: Nurhajati
authors.affiliation: Department of Animal Husbandry, Faculty of Veterinary Medicine, Airlangga University, Jl. Mulyorejo, Campus C Unair, Surabaya, Indonesia.
authors.forename: K
authors.initials: K
authors.lastname: Supranianondo
authors.affiliation: Faculty of Veterinary Medicine, Wijaya Kusuma Surabaya University, Jl. Dukuh Kupang Barat XVI/1 Surabaya, Indonesia.
authors.forename: A B
authors.initials: AB
authors.lastname: Yulianto
keywords: Enterobacter cloacae WPL 214; endo-(1,4)-β-D-glucanase; exo-(1,4)-β-D-glucanase; β-glucosidase