title: Microarray and genetic analysis reveals that csa-miR159b plays a critical role in abscisic acid-mediated heat tolerance in grafted cucumber plants.
abstract: Root-shoot communication plays a vital role in plant growth, development and adaptation to environmental stimuli. Grafting-induced stress tolerance is associated with the induction of plentiful stress-related genes and proteins; the mechanism involved, however, remains obscure. Here, we show that the enhanced tolerance against heat stress in cucumber plants with luffa as rootstock was accompanied with an increased accumulation of abscisic acid (ABA), down-regulation of a subset of microRNAs (miRNAs) but up-regulation of their target genes and CsHSP70 accumulation in the shoots. Significantly, luffa rootstock and foliar application of ABA both down-regulated csa-miR159b and up-regulated its target mRNAs CsGAMYB1 and CsMYB29-like and CsHSP70 accumulation in cucumber, while ectopic expression of csa-miR159b led to decreased heat tolerance, AtMYB33 transcript and AtHSP70 accumulation in Arabidopsis plants. Taken together, our results suggest that root-originated signals such as ABA could alter miRNAs in the shoots, which have a major role in the post-transcriptional regulation of the stress-responsive genes.
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.|College of Horticulture, Northwest A&F University, Yangling, 712100, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.
authors.forename: Ze
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.
authors.forename: Xie
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.
authors.forename: Xiao-Jian
authors.initials: XJ
authors.lastname: Xia
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Shi
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.
authors.forename: Jing-Quan
authors.initials: JQ
authors.lastname: Yu
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.|Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, 866 Yuhangtang Road, Hangzhou, 310058, China.
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Zhou
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D017360:Arabidopsis; D029681:Arabidopsis Proteins / Q000378:metabolism; D018553:Cucumis sativus / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D035683:MicroRNAs / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D018520:Plant Shoots / Q000378:metabolism; D000071436:Thermotolerance; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D018840:HSP70 Heat-Shock Proteins; D035683:MicroRNAs; C577007:Myb33 protein, Arabidopsis; D014157:Transcription Factors; D000040:Abscisic Acid
keywords: Cucumis sativus; HSP70; Luffa cylindrica; autophagy; grafting; heat