title: Perilla Oil Has Similar Protective Effects of Fish Oil on High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease and Gut Dysbiosis.
abstract: Nonalcoholic fatty liver disease (NAFLD) is the most prevalent chronic liver disease in developed countries. Recent studies indicated that the modification of gut microbiota plays an important role in the progression from simple steatosis to steatohepatitis. Epidemiological studies have demonstrated consumption of fish oil or perilla oil rich in n-3 polyunsaturated fatty acids (PUFAs) protects against NAFLD. However, the underlying mechanisms remain unclear. In the present study, we adopted 16s rRNA amplicon sequencing technique to investigate the impacts of fish oil and perilla oil on gut microbiomes modification in rats with high-fat diet- (HFD-) induced NAFLD. Both fish oil and perilla oil ameliorated HFD-induced hepatic steatosis and inflammation. In comparison with the low-fat control diet, HFD feeding significantly reduced the relative abundance of Gram-positive bacteria in the gut, which was slightly reversed by either fish oil or perilla oil. Additionally, fish oil and perilla oil consumption abrogated the elevated abundance of Prevotella and Escherichia in the gut from HFD fed animals. Interestingly, the relative abundance of antiobese Akkermansia was remarkably increased only in animals fed fish oil compared with HFD group. In conclusion, compared with fish oil, perilla oil has similar but slightly weaker potency against HFD-induced NAFLD and gut dysbiosis.
authors.affiliation: School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430023, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Tian
authors.affiliation: School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430023, China.
authors.forename: Hualin
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430023, China; School of Medicine, Jianghan University, Wuhan, Hubei, China.
authors.forename: Fahu
authors.initials: F
authors.lastname: Yuan
authors.affiliation: School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430023, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430023, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Department of Blood Transfusion, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors.forename: Lei
authors.initials: L
authors.lastname: He
authors.affiliation: School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430023, China.
authors.forename: Limei
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430023, China.
authors.forename: Zhiguo
authors.identifier: 0000-0001-7325-6478
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D059305:Diet, High-Fat / Q000009:adverse effects*; D064806:Dysbiosis / Q000188:drug therapy / Q000382:microbiology; D004925:Escherichia / Q000187:drug effects / Q000472:pathogenicity; D015525:Fatty Acids, Omega-3; D005260:Female; D005395:Fish Oils / Q000008:administration & dosage*; D006094:Gram-Positive Bacteria / Q000187:drug effects / Q000472:pathogenicity; D059014:High-Throughput Nucleotide Sequencing; D008099:Liver / Q000187:drug effects / Q000382:microbiology / Q000473:pathology; D008297:Male; D065626:Non-alcoholic Fatty Liver Disease / Q000188:drug therapy* / Q000473:pathology; D010938:Plant Oils / Q000008:administration & dosage; D018720:Prevotella / Q000187:drug effects / Q000302:isolation & purification / Q000472:pathogenicity; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D051381:Rats; D017962:alpha-Linolenic Acid / Q000008:administration & dosage*
chemical_list: D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D010938:Plant Oils; D012336:RNA, Ribosomal, 16S; D017962:alpha-Linolenic Acid; C058216:perilla seed oil