title: Mycothiol peroxidase MPx protects Corynebacterium glutamicum against acid stress by scavenging ROS.
abstract: OBJECTIVE
  To investigate mycothiol peroxidase (MPx) of Corynebacterium glutamicum that is a novel CysGPx family peroxidase using both the mycoredoxin and thioredoxin reducing systems as proton donors for peroxide detoxification and may be involved in the relief of acid stress.
  
  
  RESULTS
  A Δmpx mutant exhibited significantly decreased resistance to acid stress and markedly increased accumulation of reactive oxygen species (ROS) and protein carbonylation levels in vivo. Over-expression of mpx increased the resistance of C. glutamicum to acid stress by reducing ROS accumulation. The stress-responsive extracytoplasmic function-sigma (ECF-σ) factor, SigH, mediated acid-induced expression of mpx in the wild-type under acid conditions, which in turn directly contributed to tolerance to acid stress.
  
  
  CONCLUSIONS
  MPx is essential for combating acid stress by reducing intracellular ROS levels induced by acid stress in C. glutamicum, which adds a new dimension to the general physiological functions of CysGPx.
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Tietao
authors.initials: T
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Kang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhao
authors.affiliation: College of Life Sciences, Qufu Normal University, Qufu, 273165, Shandong, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Su
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Muhang
authors.initials: M
authors.lastname: Li
authors.affiliation: College of Life Sciences, Qufu Normal University, Qufu, 273165, Shandong, China.
authors.forename: Meiru
authors.initials: M
authors.lastname: Si
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China. xihuishen@nwsuaf.edu.cn.
authors.forename: Xihui
authors.initials: X
authors.lastname: Shen
mesh_terms: D000143:Acids / Q000494:pharmacology; D048230:Corynebacterium glutamicum / Q000187:drug effects / Q000201:enzymology*; D003545:Cysteine / Q000378:metabolism*; D006020:Glycopeptides / Q000378:metabolism*; D007294:Inositol / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism*; D050050:Protein Carbonylation; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012808:Sigma Factor / Q000378:metabolism
chemical_list: D000143:Acids; D006020:Glycopeptides; D017382:Reactive Oxygen Species; D012808:Sigma Factor; C089265:mycothiol; D007294:Inositol; D010544:Peroxidases; D003545:Cysteine
keywords: Acid stress; Corynebacterium glutamicum; Mycothiol peroxidase; Reactive oxygen species; Sigma factor (SigH)