title: [Endomorphine-1 inhibits maturation and functions of human peripheral blood-derived dendritic cells].
abstract: OBJECTIVE
  To investigate the effects of endomorphine-1 (EM-1) on the functional features of lipopolysaccharide (LPS)-stimulated peripheral blood-derived dendritic cells (PBDCs), including PBDC phenotypes, cytokine profile and its capability of promoting T cell proliferation.
  
  
  METHODS
  PBDCs were isolated from peripheral blood and differentiated in the presence of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) and interleukin-4 (rhIL-4). Then the cells were further activated by LPS or EM-1. Several surface markers were detected by flow cytometry including CD80, CD86, CD83, HLA-DR and CCR7. ELISA was used to detect cytokine levels of IL-10, IL-12, IFN-γ in the supernatants of the cultured PBDCs. Using co-culture of T lymphocytes and PBDCs stimulated by EM-1, the proliferation of T cells induced by PBDCs was determined by 5, 6-carboxyfluorescein succinimidyl ester (CFSE) staining.
  
  
  RESULTS
  EM-1 down-regulated the expressions of CD80, CD86, CD83, HLA-DR and CCR7 on the activated PBDCs. Moreover, EM-1 decreased the secretion of IL-12 and IL-10 in these PBDCs. In co-culture of T lymphocytes and EM-1-treated PBDCs, T cell proliferation was impaired, and less IL-12 and IFN-γ were produced in the culture supernatant.
  
  
  CONCLUSIONS
  EM-1 could inhibit the maturation and immune functions of PBDCs.
authors.affiliation: Department of Physiology, Bengbu Medical College, Bengbu 230030, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Physiology, Bengbu Medical College, Bengbu 230030, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Physiology, Bengbu Medical College, Bengbu 230030, China.
authors.forename: Zhiyu
authors.initials: Z
authors.lastname: Pan
authors.affiliation: Anhui Provincial Key Laboratory of Infection and Immunity, Bengbu 230030, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Anhui Provincial Key Laboratory of Infection and Immunity, Bengbu 230030, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xia
authors.affiliation: Department of Physiology, Bengbu Medical College, Bengbu 230030, China. *Corresponding author, E-mail: lizhbbmc@163.com.
authors.forename: Zhenghong
authors.initials: Z
authors.lastname: Li
mesh_terms: D000293:Adolescent; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D001773:Blood Cells / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects* / Q000276:immunology; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology; D018664:Interleukin-12 / Q000235:genetics / Q000276:immunology; D015847:Interleukin-4 / Q000235:genetics / Q000276:immunology; D008297:Male; D009842:Oligopeptides / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D055815:Young Adult
chemical_list: D015703:Antigens, CD; C508609:IL10 protein, human; C508598:IL4 protein, human; D009842:Oligopeptides; C105293:endomorphin 1; D016753:Interleukin-10; D018664:Interleukin-12; D015847:Interleukin-4