title: Molecular analysis of leptospires from serogroup Sejroe obtained from asymptomatic cattle in Rio de Janeiro - Brazil reveals genetic proximity to serovar Guaricura.
abstract: Bovine leptospirosis causes substantial reproductive failure in cattle, mainly due to infections with serovar (sv) Hardjo infection. Notwithstanding, other serovars from the serogroup (sg) Sejroe could also have important roles in bovine leptospirosis. The objective was to investigate genetic diversity of serogroup Sejroe isolates obtained from asymptomatic cattle in the state of Rio de Janeiro, Brazil. Urine and vaginal fluid (VF) were collected from clinically healthy cattle immediately after slaughter. Five isolates were recovered and characterized (serogrouping) as belonging to sg Sejroe. Sequencing of rrs and secY genes further identified them as Leptospira santarosai. Analysis of secY sequences indicated a high level of sequence homology to sv Guaricura strains. Based on culture and sequence data, we inferred that other members of sg Sejroe may be important in bovine leptospiral infection, particularly genotypes of L. santarosai serovar Guaricura.
authors.affiliation: Laboratório de Bacteriologia Veterinária, Departamento de Microbiologia e Parasitologia, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.
authors.forename: A P
authors.initials: AP
authors.lastname: Loureiro
authors.affiliation: Laboratório de Bacteriologia Veterinária, Departamento de Microbiologia e Parasitologia, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Hamond
authors.affiliation: Laboratório de Bacteriologia Veterinária, Departamento de Microbiologia e Parasitologia, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.
authors.forename: P
authors.initials: P
authors.lastname: Pinto
authors.affiliation: Institut Pasteur, Unité de Biologie des Spirochètes, National Reference Center and WHO Collaborating Center for Leptospirosis, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Bremont
authors.affiliation: Institut Pasteur, Unité de Biologie des Spirochètes, National Reference Center and WHO Collaborating Center for Leptospirosis, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Bourhy
authors.affiliation: Laboratório de Bacteriologia Veterinária, Departamento de Microbiologia e Parasitologia, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil. Electronic address: mipwalt@vm.uff.br.
authors.forename: W
authors.initials: W
authors.lastname: Lilenbaum
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001938:Brazil; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D005260:Female; D014644:Genetic Variation; D007919:Leptospira / Q000235:genetics* / Q000378:metabolism; D007922:Leptospirosis / Q000382:microbiology / Q000662:veterinary*; D008297:Male; D010802:Phylogeny; D016415:Sequence Alignment / Q000662:veterinary
keywords: Cattle; Guaricura; Sejroe; Serogrouping; rrs; secY