title: Clenbuterol changes phosphorylated FOXO1 localization and decreases protein degradation in the sartorius muscle of neonatal chicks.
abstract: To investigate the intracellular signaling mechanisms by which clenbuterol reduces muscle protein degradation, we examined the phosphorylation level and intracellular localization of FOXO1 in the sartorius muscle of neonatal chicks. One-day-old chicks were given a single intraperitoneal injection of clenbuterol (0.1 mg/kg body weight). Three hours after injection, AKT protein was phosphorylated in the sartorius muscle by clenbuterol injection. Coincidentally, clenbuterol increased cytosolic level of phosphorylated FOXO1 protein, while it decreased nuclear level of FOXO1 protein in the sartorius muscle. Furthermore, clenbuterol decreased the expression of mRNAs for muscle-specific ubiquitin ligases (atrogin-1/MAFbx and MuRF1) in the sartorius muscle accompanied by decreased plasma 3-methylhistidine concentration, an index of muscle protein degradation, at 3 h after injection. These results suggested that, in the sartorius muscle of the chicks, clenbuterol changed the intracellular localization of phosphorylated FOXO1, and consequently decreased protein degradation via suppressing the expression of genes encoding muscle-specific ubiquitin ligases.
authors.affiliation: a Department of Biochemical Science and Technology , Kagoshima University , Kagoshima , Japan.
authors.forename: Saki
authors.initials: S
authors.lastname: Shimamoto
authors.affiliation: a Department of Biochemical Science and Technology , Kagoshima University , Kagoshima , Japan.
authors.forename: Daichi
authors.initials: D
authors.lastname: Ijiri
authors.affiliation: b Animal Physiology and Nutrition Division , NARO Institute of Livestock and Grassland Science , Tsukuba , Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Nakashima
authors.affiliation: a Department of Biochemical Science and Technology , Kagoshima University , Kagoshima , Japan.
authors.forename: Mana
authors.initials: M
authors.lastname: Kawaguchi
authors.affiliation: a Department of Biochemical Science and Technology , Kagoshima University , Kagoshima , Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Ishimaru
authors.affiliation: a Department of Biochemical Science and Technology , Kagoshima University , Kagoshima , Japan.
authors.forename: Airi
authors.initials: A
authors.lastname: Furukawa
authors.affiliation: a Department of Biochemical Science and Technology , Kagoshima University , Kagoshima , Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Ohtsuka
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D030161:Avian Proteins / Q000235:genetics* / Q000378:metabolism; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D002645:Chickens; D002976:Clenbuterol / Q000494:pharmacology*; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D000071161:Forkhead Box Protein O1 / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D007274:Injections, Intraperitoneal; D008762:Methylhistidines / Q000097:blood; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D059748:Proteolysis / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism; D044843:SKP Cullin F-Box Protein Ligases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D013566:Sympathomimetics / Q000494:pharmacology*; D044767:Ubiquitin-Protein Ligases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism
chemical_list: D030161:Avian Proteins; D000071161:Forkhead Box Protein O1; D008762:Methylhistidines; D013566:Sympathomimetics; D044843:SKP Cullin F-Box Protein Ligases; D044767:Ubiquitin-Protein Ligases; D051057:Proto-Oncogene Proteins c-akt; C028118:3-methylhistidine; D002976:Clenbuterol
keywords: FOXO transcription factor; clenbuterol; skeletal muscle; β2-adrenergic receptor