title: A Carbapenem-Resistant Pseudomonas aeruginosa Isolate Harboring Two Copies of blaIMP-34 Encoding a Metallo-β-Lactamase.
abstract: A carbapenem-resistant strain of Pseudomonas aeruginosa, NCGM1984, was isolated in 2012 from a hospitalized patient in Japan. Immunochromatographic assay showed that the isolate was positive for IMP-type metallo-β-lactamase. Complete genome sequencing revealed that NCGM1984 harbored two copies of blaIMP-34, located at different sites on the chromosome. Each blaIMP-34 was present in the same structures of the class 1 integrons, tnpA(ISPa7)-intI1-qacG-blaIMP-34-aac(6')-Ib-qacEdelta1-sul1-orf5-tniBdelta-tniA. The isolate belonged to multilocus sequence typing ST235, one of the international high-risk clones. IMP-34, with an amino acid substitution (Glu126Gly) compared with IMP-1, hydrolyzed all β-lactamases tested except aztreonam, and its catalytic activities were similar to IMP-1. This is the first report of a clinical isolate of an IMP-34-producing P. aeruginosa harboring two copies of blaIMP-34 on its chromosome.
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku, Tokyo, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Tada
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku, Tokyo, Japan.|Pathogenic Microbe Laboratory, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku, Tokyo, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Miyoshi-Akiyama
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku, Tokyo, Japan.
authors.forename: Kayo
authors.initials: K
authors.lastname: Shimada
authors.affiliation: Research and Development Division, Okinawa Institute of Advanced Sciences, Uruma, Okinawa, Japan.
authors.forename: Akino
authors.initials: A
authors.lastname: Shiroma
authors.affiliation: Research and Development Division, Okinawa Institute of Advanced Sciences, Uruma, Okinawa, Japan.
authors.forename: Kazuma
authors.initials: K
authors.lastname: Nakano
authors.affiliation: Research and Development Division, Okinawa Institute of Advanced Sciences, Uruma, Okinawa, Japan.
authors.forename: Kuniko
authors.initials: K
authors.lastname: Teruya
authors.affiliation: Research and Development Division, Okinawa Institute of Advanced Sciences, Uruma, Okinawa, Japan.
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Satou
authors.affiliation: Research and Development Division, Okinawa Institute of Advanced Sciences, Uruma, Okinawa, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Hirano
authors.affiliation: Department of Accuracy Management, BML Inc., Kawagoe, Saitama, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Shimojima
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku, Tokyo, Japan.
authors.forename: Teruo
authors.initials: T
authors.lastname: Kirikae
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D015780:Carbapenems / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016680:Genome, Bacterial; D006801:Humans; D007564:Japan; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D016133:Polymerase Chain Reaction; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000235:genetics* / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000302:isolation & purification*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D015780:Carbapenems; D004269:DNA, Bacterial; D001618:beta-Lactamases