title: [Relationship between the expression of immunoglobulin heavy chain binding protein and calreticulin and metastasis in hepatocellular carcinoma].
abstract: OBJECTIVE
  To investigate the relationship between the expression of immunoglobulin heavy chain binding protein (Bip) and calreticulin (CRT) and metastasis in hepatocellular carcinoma.
  
  
  METHODS
  Western blot and RT-qPCR were used to detect the expression of Bip and CRT in normal hepatic cells and hepatocellular carcinoma cells. 43 cases of hepatocellular carcinoma tissues were collected from the First Affiliated Hospital of Zhengzhou University between June 2014 and May 2015, Western blot and immunohistochemistry assay were used to detect the expression of Bip and CRT in hepatocellular carcinoma tissues, adjacent non-cancer tissues, 5 cases adjacent liver hemangioma tissues and the clinical significance was also analyzed.
  
  
  RESULTS
  The expression of Bip and CRT in hepatocellular carcinoma cells and hepatocellular carcinoma tissues was higher than normal hepatic cells and adjacent non-cancer tissues and also adjacent liver hemangioma tissues. The positive rate of Bip and CRT were 86.0%(37/43) and 65.1%(28/43) in 43 hepatocellular carcinoma tissues, which was significantly higher than those adjacent non-cancer tissues and adjacent liver hemangioma tissues (P<0.05). The positive rate of Bip and CRT in metastatic cancer tissues were 100%(19/19), 84.2%(16/19), respectively, which was significantly higher than those non-metastatic cancer tissues 75.0%(18/24), 50.0%(12/24) respectively (P<0.05). The expression of Bip and CRT was positively correlated (r=0.382, P<0.05). The positive rate and relative expression of Bip were correlated with TNM stage, metastasis in hepatocellular carcinoma tissues (P<0.05), while the positive rate and relative expression of CRT were correlated with metastasis in hepatocellular carcinoma tissues (P<0.05).
  
  
  CONCLUSIONS
  The expression of Bip and CRT is correlation with metastasis in hepatocellular carcinoma.
authors.affiliation: Department of Hepatobiliary and Pancreatic Surgery, the First Affiliated Hospital of Zhengzhou University; Key Laboratory of Hepatobiliary and Pancreatic Surgery & Digestive Organ Transplantation of Henan Province, Zhengzhou 450052, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Fu
authors.forename: Jianwen
authors.initials: J
authors.lastname: Ye
authors.forename: Wenchao
authors.initials: W
authors.lastname: Tang
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.forename: Hongwei
authors.initials: H
authors.lastname: Tang
authors.forename: Bing
authors.initials: B
authors.lastname: Yan
authors.forename: Ruo
authors.initials: R
authors.lastname: Feng
mesh_terms: D015153:Blotting, Western; D037282:Calreticulin; D006528:Carcinoma, Hepatocellular; D006391:Hemangioma; D006801:Humans; D007143:Immunoglobulin Heavy Chains; D007150:Immunohistochemistry; D008113:Liver Neoplasms; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C576483:CALR protein, human; D037282:Calreticulin; D007143:Immunoglobulin Heavy Chains