title: Transcriptome Analysis of Spartina pectinata in Response to Freezing Stress.
abstract: Prairie cordgrass (Spartina pectinata), a perennial C4 grass native to the North American prairie, has several distinctive characteristics that potentially make it a model crop for production in stressful environments. However, little is known about the transcriptome dynamics of prairie cordgrass despite its unique freezing stress tolerance. Therefore, the purpose of this work was to explore the transcriptome dynamics of prairie cordgrass in response to freezing stress at -5°C for 5 min and 30 min. We used a RNA-sequencing method to assemble the S. pectinata leaf transcriptome and performed gene-expression profiling of the transcripts under freezing treatment. Six differentially expressed gene (DEG) groups were categorized from the profiling. In addition, two major consecutive orders of gene expression were observed in response to freezing; the first being the acute up-regulation of genes involved in plasma membrane modification, calcium-mediated signaling, proteasome-related proteins, and transcription regulators (e.g., MYB and WRKY). The follow-up and second response was of genes involved in encoding the putative anti-freezing protein and the previously known DNA and cell-damage-repair proteins. Moreover, we identified the genes involved in epigenetic regulation and circadian-clock expression. Our results indicate that freezing response in S. pectinata reflects dynamic changes in rapid-time duration, as well as in metabolic, transcriptional, post-translational, and epigenetic regulation.
authors.affiliation: Department of Plant Science, Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, 599 Gwanangno, Gwanakgu, Seoul 08826, Republic of Korea.
authors.forename: Gyoungju
authors.initials: G
authors.lastname: Nah
authors.affiliation: Department of Crop Sciences, University of Illinois at Urbana-Champaign, 1120 S. Goodwin Ave, Urbana, Illinois 61801, United States of America.
authors.forename: Moonsub
authors.initials: M
authors.lastname: Lee
authors.affiliation: Department of Plant Science, Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, 599 Gwanangno, Gwanakgu, Seoul 08826, Republic of Korea.
authors.forename: Do-Soon
authors.initials: DS
authors.lastname: Kim
authors.affiliation: Department of Crop Sciences, University of Illinois at Urbana-Champaign, 1120 S. Goodwin Ave, Urbana, Illinois 61801, United States of America.
authors.forename: A Lane
authors.initials: AL
authors.lastname: Rayburn
authors.affiliation: Department of Crop Sciences, University of Illinois at Urbana-Champaign, 1120 S. Goodwin Ave, Urbana, Illinois 61801, United States of America.
authors.forename: Thomas
authors.initials: T
authors.lastname: Voigt
authors.affiliation: Department of Plant Science, Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, 599 Gwanangno, Gwanakgu, Seoul 08826, Republic of Korea.|Department of Crop Sciences, University of Illinois at Urbana-Champaign, 1120 S. Goodwin Ave, Urbana, Illinois 61801, United States of America.
authors.forename: D K
authors.initials: DK
authors.lastname: Lee
mesh_terms: D058639:Cold-Shock Response; D044127:Epigenesis, Genetic; D005615:Freezing; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D006109:Poaceae / Q000235:genetics* / Q000502:physiology