title: Differential Impacts of Soybean and Fish Oils on Hepatocyte Lipid Droplet Accumulation and Endoplasmic Reticulum Stress in Primary Rabbit Hepatocytes.
abstract: Parenteral nutrition-associated liver disease (PNALD) is a severe ailment associated with long-term parenteral nutrition. Soybean oil-based lipid emulsions (SOLE) are thought to promote PNALD development, whereas fish oil-based lipid emulsions (FOLE) are thought to protect against PNALD. This study aimed to investigate the effects of SOLE and FOLE on primary rabbit hepatocytes. The results reveal that SOLE caused significant endoplasmic reticulum (ER) and mitochondrial damage, ultimately resulting in lipid droplets accumulation and ER stress. While these deleterious events induce hepatocyte injury, FOLE at high doses cause only minor ER and mitochondrial damage, which has no effect on hepatic function. SOLE also significantly upregulated glucose-regulated protein 94 mRNA and protein expression. These data indicate that SOLE, but not FOLE, damage the ER and mitochondria, resulting in lipid droplets accumulation and ER stress and, finally, hepatocyte injury. This likely contributes to the differential impacts of SOLE and FOLE on PNALD development and progression.
authors.affiliation: Department of Neonatology, Children's Hospital of Soochow University, Suzhou, Jiangsu 215003, China.
authors.forename: Xueping
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Neonatology, Children's Hospital of Soochow University, Suzhou, Jiangsu 215003, China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: Department of Neonatology, Children's Hospital of Soochow University, Suzhou, Jiangsu 215003, China.
authors.forename: Yumin
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Neonatology, Children's Hospital of Soochow University, Suzhou, Jiangsu 215003, China.
authors.forename: Xingli
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Neonatology, Children's Hospital of Soochow University, Suzhou, Jiangsu 215003, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Cheng
authors.affiliation: Department of Neonatology, Children's Hospital of Soochow University, Suzhou, Jiangsu 215003, China.
authors.forename: Ningxun
authors.initials: N
authors.lastname: Cui
authors.affiliation: Department of Neonatology, Children's Hospital of Soochow University, Suzhou, Jiangsu 215003, China.
authors.forename: Mingling
authors.initials: M
authors.lastname: Cui
authors.affiliation: Department of Neonatology, Children's Hospital of Soochow University, Suzhou, Jiangsu 215003, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Intervention, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhu