title: Role of HIF-1 on phosphofructokinase and fructose 1, 6-bisphosphatase expression during hypoxia in the white shrimp Litopenaeus vannamei.
abstract: HIF-1 is a transcription factor that controls a widespread range of genes in metazoan organisms in response to hypoxia and is composed of α and β subunits. In shrimp, phosphofructokinase (PFK) and fructose bisphosphatase (FBP) are up-regulated in hypoxia. We hypothesized that HIF-1 is involved in the regulation of PFK and FBP genes in shrimp hepatopancreas under hypoxia. Long double stranded RNA (dsRNA) intramuscular injection was utilized to silence simultaneously both HIF-1 subunits, and then, we measured the relative expression of PFK and FBP, as well as their corresponding enzymatic activities in hypoxic shrimp hepatopancreas. The results indicated that HIF-1 participates in the up-regulation of PFK transcripts under short-term hypoxia since the induction caused by hypoxia (~1.6 and ~4.2-fold after 3 and 48h, respectively) is significantly reduced in the dsRNA animals treated. Moreover, PFK activity was significantly ~2.8-fold augmented after 3h in hypoxia alongside to an ~1.9-fold increment in lactate. However, when animals were dsRNA treated, both were significantly reduced. On the other hand, FBP transcripts were ~5.3-fold up-regulated in long-term hypoxic conditions (48h). HIF-1 is involved in this process since FBP transcripts were not induced by hypoxia when HIF-1 was silenced. Conversely, the FBP activity was not affected by hypoxia, which suggests its possible regulation at post-translational level. Taken together, these results position HIF-1 as a prime transcription factor in coordinating glucose metabolism through the PFK and FBP genes among others, in shrimp under low oxygen environments.
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C., P.O. Box 1735, Carretera a Ejido La Victoria Km. 0.6 Hermosillo, Sonora 83304, México.
authors.forename: Keni
authors.initials: K
authors.lastname: Cota-Ruiz
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C., P.O. Box 1735, Carretera a Ejido La Victoria Km. 0.6 Hermosillo, Sonora 83304, México.
authors.forename: Lilia
authors.initials: L
authors.lastname: Leyva-Carrillo
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C., P.O. Box 1735, Carretera a Ejido La Victoria Km. 0.6 Hermosillo, Sonora 83304, México.
authors.forename: Alma B
authors.initials: AB
authors.lastname: Peregrino-Uriarte
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C., P.O. Box 1735, Carretera a Ejido La Victoria Km. 0.6 Hermosillo, Sonora 83304, México.
authors.forename: Elisa M
authors.initials: EM
authors.lastname: Valenzuela-Soto
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C., P.O. Box 1735, Carretera a Ejido La Victoria Km. 0.6 Hermosillo, Sonora 83304, México.
authors.forename: Teresa
authors.initials: T
authors.lastname: Gollas-Galván
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C., P.O. Box 1735, Carretera a Ejido La Victoria Km. 0.6 Hermosillo, Sonora 83304, México.
authors.forename: Silvia
authors.initials: S
authors.lastname: Gómez-Jiménez
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C., P.O. Box 1735, Carretera a Ejido La Victoria Km. 0.6 Hermosillo, Sonora 83304, México.
authors.forename: Jesús
authors.initials: J
authors.lastname: Hernández
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C., P.O. Box 1735, Carretera a Ejido La Victoria Km. 0.6 Hermosillo, Sonora 83304, México. Electronic address: gyepiz@ciad.mx.
authors.forename: Gloria
authors.initials: G
authors.lastname: Yepiz-Plascencia
mesh_terms: D000818:Animals; D006597:Fructose-Bisphosphatase / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D055785:Gene Knockdown Techniques; D043143:Hepatopancreas / Q000378:metabolism; D000860:Hypoxia; D051793:Hypoxia-Inducible Factor 1 / Q000235:genetics / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism; D033561:Penaeidae / Q000502:physiology*; D025221:Phosphofructokinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D051793:Hypoxia-Inducible Factor 1; D007773:Lactates; D025221:Phosphofructokinases; D006597:Fructose-Bisphosphatase
keywords: 6-Bisphosphatase (FBP); Fructose 1; Gene silencing; HIF-1; Hypoxia; Phosphofructokinase (PFK); Shrimp