title: Purification of Lysosomes Using Supraparamagnetic Iron Oxide Nanoparticles (SPIONs).
abstract: Lysosomes can be rapidly isolated from tissue culture cells using supraparamagnetic iron oxide particles (SPIONs). In this protocol, colloidal iron dextran (FeDex) particles, a type of SPION, are taken up by cultured mouse macrophage cells via the endocytic pathway. The SPIONs accumulate in lysosomes, the end point of the endocytic pathway, permitting the lysosomes to be isolated magnetically. The purified lysosomes are suitable for in vitro fusion assays or for proteomic analysis.
authors.affiliation: Centre for Immunology and Infection, Hull York Medical School and Department of Biology, University of York, York YO10 5DD, United Kingdom.
authors.forename: Adam P
authors.initials: AP
authors.lastname: Rofe
authors.affiliation: Centre for Immunology and Infection, Hull York Medical School and Department of Biology, University of York, York YO10 5DD, United Kingdom.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Pryor
mesh_terms: D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002460:Cell Line; D004705:Endocytosis; D005290:Ferric Compounds / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D008280:Magnetics; D051379:Mice; D053758:Nanoparticles / Q000378:metabolism*
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide