title: Benzodiazepine inhibits anxiogenic-like response in cocaine or ethanol withdrawn planarians.
abstract: Planarians spend less time in light versus dark environments. We hypothesized that planarians withdrawn from cocaine or ethanol would spend even less time in the light than drug-naive planarians and that a benzodiazepine would inhibit this response. Planarians pretreated in cocaine or ethanol were placed at the midline of a Petri dish containing spring water that was split evenly into dark and light compartments. Planarians withdrawn from cocaine (1, 10, 100 μmol/l) or ethanol (0.01%) spent less time in the light compartment than water controls; however, this withdrawal response to cocaine (100 μmol/l) or ethanol (0.01%) was abolished by clorazepate (0-100 μmol/l). These data suggest that planarians, similar to rodents, show benzodiazepine-sensitive, anxiogenic-like responses during cocaine or alcohol withdrawal.
authors.affiliation: aCenter for Substance Abuse Research bDepartment of Pharmacology, Temple University School of Medicine, Philadelphia, Pennsylvania, USA.
authors.forename: Sunil
authors.initials: S
authors.lastname: Nayak
authors.forename: Adam
authors.initials: A
authors.lastname: Roberts
authors.forename: Kristofer
authors.initials: K
authors.lastname: Bires
authors.forename: Christopher S
authors.initials: CS
authors.lastname: Tallarida
authors.forename: Erin
authors.initials: E
authors.lastname: Kim
authors.forename: Michael
authors.initials: M
authors.lastname: Wu
authors.forename: Scott M
authors.initials: SM
authors.lastname: Rawls
mesh_terms: D000818:Animals; D014151:Anti-Anxiety Agents / Q000008:administration & dosage / Q000494:pharmacology; D001522:Behavior, Animal / Q000187:drug effects; D001569:Benzodiazepines / Q000008:administration & dosage / Q000494:pharmacology*; D003009:Clorazepate Dipotassium / Q000008:administration & dosage / Q000494:pharmacology*; D003042:Cocaine / Q000008:administration & dosage*; D003624:Darkness; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000008:administration & dosage*; D008027:Light; D010932:Planarians; D013375:Substance Withdrawal Syndrome; D013997:Time Factors
chemical_list: D014151:Anti-Anxiety Agents; D001569:Benzodiazepines; D000431:Ethanol; D003009:Clorazepate Dipotassium; D003042:Cocaine