title: Adaptive responses of outer membrane porin balance of Yersinia ruckeri under different incubation temperature, osmolarity, and oxygen availability.
abstract: The capability of Yersinia ruckeri to survive in the aquatic systems reflects its adaptation (most importantly through the alteration of membrane permeability) to the unfavorable environments. The nonspecific porins are a key factor contributing to the permeability. Here we studied the influence of the stimuli, such as temperature, osmolarity, and oxygen availability on regulation of Y. ruckeri porins. Using qRT-PCR and SDS-PAGE methods we found that major porins are tightly controlled by temperature. Hyperosmosis did not repress OmpF production. The limitation of oxygen availability led to decreased expression of both major porins and increased transcription of the minor porin OmpY. Regulation of the porin balance in Y. ruckeri, in spite of some similarities, diverges from that system in Escherichia coli. The changes in porin regulation can be adapted in Y. ruckeri in a species-specific manner determined by its aquatic habitats.
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry FEB RAS, 690022 Prospect 100-let Vladivostoku 159, Vladivostok, Russia.|The School of Biomedicine, Far Eastern Federal University, 690950 Suchanova str. 8, Vladivostok, Russia.
authors.forename: Evgeniya
authors.initials: E
authors.lastname: Bystritskaya
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry FEB RAS, 690022 Prospect 100-let Vladivostoku 159, Vladivostok, Russia.|The School of Biomedicine, Far Eastern Federal University, 690950 Suchanova str. 8, Vladivostok, Russia.
authors.forename: Anna
authors.initials: A
authors.lastname: Stenkova
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry FEB RAS, 690022 Prospect 100-let Vladivostoku 159, Vladivostok, Russia.
authors.forename: Dmitriy
authors.initials: D
authors.lastname: Chistuylin
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry FEB RAS, 690022 Prospect 100-let Vladivostoku 159, Vladivostok, Russia.|The School of Biomedicine, Far Eastern Federal University, 690950 Suchanova str. 8, Vladivostok, Russia.
authors.forename: Nadezhda
authors.initials: N
authors.lastname: Chernysheva
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry FEB RAS, 690022 Prospect 100-let Vladivostoku 159, Vladivostok, Russia.
authors.forename: Valentina
authors.initials: V
authors.lastname: Khomenko
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry FEB RAS, 690022 Prospect 100-let Vladivostoku 159, Vladivostok, Russia.
authors.forename: Stanislav
authors.initials: S
authors.lastname: Anastyuk
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry FEB RAS, 690022 Prospect 100-let Vladivostoku 159, Vladivostok, Russia.
authors.forename: Olga
authors.initials: O
authors.lastname: Novikova
authors.affiliation: Max von Pettenkofer Institute for Hygiene and Clinical Microbiology of Ludwig Maximilians-University, 80336 Pettenkofer str. 9a, Munich, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Rakin
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry FEB RAS, 690022 Prospect 100-let Vladivostoku 159, Vladivostok, Russia.|The School of Biomedicine, Far Eastern Federal University, 690950 Suchanova str. 8, Vladivostok, Russia.
authors.forename: Marina
authors.initials: M
authors.lastname: Isaeva
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology*; D000693:Anaerobiosis / Q000502:physiology; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000502:physiology; D002463:Cell Membrane Permeability / Q000502:physiology*; D004926:Escherichia coli / Q000378:metabolism; D005393:Fish Diseases / Q000382:microbiology / Q000473:pathology; D005399:Fishes / Q000382:microbiology; D015964:Gene Expression Regulation, Bacterial; D009994:Osmolar Concentration; D010100:Oxygen / Q000378:metabolism*; D018272:Porins / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature; D014871:Water Microbiology; D044124:Yersinia ruckeri / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; C109237:OmpF protein; D018272:Porins; D010100:Oxygen
keywords: Bacteria; environmental signal/stress responses; gene expression/regulation; outer membrane proteins; porins.