title: Variations in grain lipophilic phytochemicals, proteins and resistance to Fusarium spp. growth during grain storage as affected by biological plant protection with Aureobasidium pullulans (de Bary).
abstract: Modern agriculture relies on an integrated approach, where chemical treatment is reduced to a minimum and replaced by biological control that involves the use of active microorganisms. The effect of the antagonistic yeast-like fungus Aureobasidium pullulans on proteins and bioactive compounds (alkylresorcinols, sterols, tocols and carotenoids) in winter wheat grain and on the colonization of wheat kernels by fungal microbiota, mainly Fusarium spp. pathogens, was investigated. Biological treatment contributed to a slight increase contents of tocols, alkylresorcinols and sterols in grain. At the same time, the variation of wheat grain proteins was low and not significant. Application of A. pullulans enhanced the natural yeast colonization after six months of grain storage and inhibited growth of F. culmorum pathogens penetrating wheat kernel. This study demonstrated that an integrated approach of wheat grain protection with the use of the yeast-like fungus A. pullulans reduced kernel colonization by Fusarium spp. pathogens and increased the content of nutritionally beneficial phytochemicals in wheat grain without a loss of gluten proteins responsible for baking value.
authors.affiliation: Department of Entomology, Phytopathology and Molecular Diagnostics, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 17, 10-720 Olsztyn, Poland. Electronic address: urszula.wachowska@uwm.edu.pl.
authors.forename: Urszula
authors.initials: U
authors.lastname: Wachowska
authors.affiliation: Chair of Plant Food Chemistry and Processing of Faculty of Food Sciences, University of Warmia and Mazury in Olsztyn, pl. Cieszyński 1, 10-726 Olsztyn, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Tańska
authors.affiliation: Chair of Plant Food Chemistry and Processing of Faculty of Food Sciences, University of Warmia and Mazury in Olsztyn, pl. Cieszyński 1, 10-726 Olsztyn, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Konopka
mesh_terms: D001203:Ascomycota; D061046:Biological Control Agents; D002523:Edible Grain / Q000382:microbiology; D061353:Food Storage; D005670:Fusarium / Q000502:physiology*; D064209:Phytochemicals / Q000032:analysis; D010935:Plant Diseases / Q000382:microbiology*; D014908:Triticum / Q000737:chemistry* / Q000382:microbiology*
chemical_list: D061046:Biological Control Agents; D064209:Phytochemicals
keywords: Aureobasidium pullulans; Bioactive compounds; Biological control; Fusarium spp.; Gluten proteins