title: [Investigation of human herpesvirus-8 seroprevalence in blood donors and HIV-positive patients admitted to Ege University Medical School Hospital, Turkey].
abstract: Human herpesvirus 8 (HHV-8), classified in Herpesviridae family, is the etiological agent of Kaposi's sarcoma (KS), primary effusion lymphoma and multicentric Castleman's disease. In contrast to the other herpesviruses, HHV-8 seroprevalence is low in general populations; however, the higher prevalence observed in individuals with immunodeficiencies such as AIDS poses an increased risk for KS. The global distribution of HHV-8 shows great variations, with the highest seroprevalence seen in Africa. The number of studies on the seroprevalence of HHV-8 in Turkey are limited. The aim of this study was to determine the HHV-8 seroprevalences in healthy blood donors and HIV-positive patients, that will contribute HHV-8 seroepidemiological data in our country. This study was designed as a cross-sectional study. A total of 551 healthy donors (76 female, 475 male; age range: 18-65 years) admitted to Ege University Medical School Hospital, Blood Center for blood donation between December 2013-January 2014, and 173 HIV-positive patients (30 female, 143 male; age range: 18-65 years) admitted to infectious diseases outpatient clinic between October 2013-January 2014, were included in the study. A commercial ELISA method (KSHV/HHV-8 IgG ELISA Kit, Advanced Biotechnologies Inc, USA) was used for the detection of IgG antibodies that were structured against HHV-8 lytic antigens. In the study, 29 (29/551, 5.3%) of blood donors and 44 (44/173, 25.4%) of HIV-positive patients, with a total of 73 (73/724, 10.1%) cases were found as HHV-8 seropositive. The difference between blood donors and HIV-positive patients in terms of HHV-8 seropositivity rates was statistically significant (5.3% versus 25.4%; p< 0.05). In both of the study groups, no statistically significant difference was detected between HHV-8 seropositivity with gender and age. When considering HIV-positive patients, no statistically significant difference was observed between HHV-8 seropositivity with the duration of anti-HIV positivity, CD4(+) T cell count, HIV-RNA status and history of having sexually transmitted disease. As a result, HHV-8 seroprevalence rate detected in our study is similar to the data of other studies performed in Turkey, as well as the rates reported from other European and Asian countries.
authors.forename: İmre
authors.initials: İ
authors.lastname: Altuğlu
authors.affiliation: Ege University Faculty of Medicine, Department of Medical Microbiology, Izmir,Turkey. dr.ayfer.bakir@gmail.com.
authors.forename: Ayfer
authors.initials: A
authors.lastname: Yolcu
authors.forename: Zeliha Aslı
authors.initials: ZA
authors.lastname: Öcek
authors.forename: Rüçhan
authors.initials: R
authors.lastname: Yazan Sertöz
authors.forename: Deniz
authors.initials: D
authors.lastname: Gökengin
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000097:blood*; D001782:Blood Donors; D005871:Castleman Disease / Q000453:epidemiology / Q000821:virology; D003430:Cross-Sectional Studies; D005260:Female; D015658:HIV Infections / Q000150:complications*; D006566:Herpesviridae Infections / Q000150:complications / Q000453:epidemiology* / Q000276:immunology; D019288:Herpesvirus 8, Human / Q000276:immunology*; D006785:Hospitals, University; D006801:Humans; D054685:Lymphoma, Primary Effusion / Q000453:epidemiology / Q000821:virology; D008297:Male; D008875:Middle Aged; D012514:Sarcoma, Kaposi / Q000453:epidemiology / Q000821:virology; D016036:Seroepidemiologic Studies; D014421:Turkey / Q000453:epidemiology; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral