title: Upregulation of microrna-451 increases the sensitivity of A549 cells to radiotherapy through enhancement of apoptosis.
abstract: BACKGROUND
  As radioresistance of non-small cell lung cancers (NSCLC) is one of the main causes of failure in radiotherapy, we examined whether micro ribonucleic acid (miR-451) could function as a potential radiosensitizer of NSCLC and the related mechanism.
  
  
  METHODS
  Radioresistant NSCLC cell line A549 was transfected with pre-miR-451 or a scrambled control. The miR-451 messenger RNA level, colony-forming ability, apoptosis, and phosphatase and tensin homolog (PTEN) protein level of 549 cells were examined by real-time polymerase chain reaction, clonogenic assay, flow cytometry analysis, and Western blot.
  
  
  RESULTS
  Upregulation of miR-451 enhanced the suppressive effects of irradiation on the colony-forming ability of A549 cells. The apoptosis and PTEN expression of A549 cells post-irradiation were also enhanced by upregulation of miR-451.
  
  
  CONCLUSIONS
  Upregulation of miR-451 sensitized radioresistant NSCLC A549 cells to irradiation through the enhancement of apoptosis. The activation of PTEN post-irradiation was possibly correlated with the radiosensitization of A549 cells induced by miR-451 overexpression.
authors.affiliation: Department of Thoracic Surgery Tangdu Hospital the Forth Military Medical University Xi'an China.
authors.forename: Feng
authors.initials: F
authors.lastname: Tian
authors.affiliation: Department of Thoracic Surgery Tangdu Hospital the Forth Military Medical University Xi'an China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Han
authors.affiliation: Department of Thoracic Surgery Tangdu Hospital the Forth Military Medical University Xi'an China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Yan
authors.affiliation: Department of Thoracic Surgery Tangdu Hospital the Forth Military Medical University Xi'an China.
authors.forename: Daixing
authors.initials: D
authors.lastname: Zhong
authors.affiliation: Department of Thoracic Surgery Tangdu Hospital the Forth Military Medical University Xi'an China.
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of Thoracic Surgery Tangdu Hospital the Forth Military Medical University Xi'an China.
authors.forename: Jie
authors.initials: J
authors.lastname: Lei
authors.affiliation: Department of Thoracic Surgery Tangdu Hospital the Forth Military Medical University Xi'an China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Thoracic Surgery Tangdu Hospital the Forth Military Medical University Xi'an China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Wang
keywords: Apoptosis; PTEN; miR‐451; non‐small cell lung cancer; radiosensitization