title: Coronin 1B Reorganizes the Architecture of F-Actin Networks for Contractility at Steady-State and Apoptotic Adherens Junctions.
abstract: In this study we sought to identify how contractility at adherens junctions influences apoptotic cell extrusion. We first found that the generation of effective contractility at steady-state junctions entails a process of architectural reorganization whereby filaments that are initially generated as poorly organized networks of short bundles are then converted into co-aligned perijunctional bundles. Reorganization requires coronin 1B, which is recruited to junctions by E-cadherin adhesion and is necessary to establish contractile tension at the zonula adherens. When cells undergo apoptosis within an epithelial monolayer, coronin 1B is also recruited to the junctional cortex at the apoptotic/neighbor cell interface in an E-cadherin-dependent fashion to support actin architectural reorganization, contractility, and extrusion. We propose that contractile stress transmitted from the apoptotic cell through E-cadherin adhesions elicits a mechanosensitive response in neighbor cells that is necessary for the morphogenetic event of apoptotic extrusion to occur.
authors.affiliation: Division of Cell Biology and Molecular Medicine, Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Brisbane, QLD 4072, Australia; Randall Division of Cell and Molecular Biophysics, King's College London, Guy's Campus, London SE1 1UL, UK.
authors.forename: Magdalene
authors.initials: M
authors.lastname: Michael
authors.affiliation: Division of Cell Biology and Molecular Medicine, Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Brisbane, QLD 4072, Australia.
authors.forename: Joyce C M
authors.initials: JCM
authors.lastname: Meiring
authors.affiliation: Division of Cell Biology and Molecular Medicine, Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Brisbane, QLD 4072, Australia.
authors.forename: Bipul R
authors.initials: BR
authors.lastname: Acharya
authors.affiliation: Queensland Brain Institute, The University of Queensland, St. Lucia, Brisbane, QLD 4072, Australia.
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Matthews
authors.affiliation: Division of Cell Biology and Molecular Medicine, Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Brisbane, QLD 4072, Australia.
authors.forename: Suzie
authors.initials: S
authors.lastname: Verma
authors.affiliation: Division of Cell Biology and Molecular Medicine, Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Brisbane, QLD 4072, Australia.
authors.forename: Siew Ping
authors.initials: SP
authors.lastname: Han
authors.affiliation: University of Queensland Diamantina Institute, Translational Research Institute, The University of Queensland, Woolloongabba, Brisbane, QLD 4102, Australia.
authors.forename: Michelle M
authors.initials: MM
authors.lastname: Hill
authors.affiliation: Division of Cell Biology and Molecular Medicine, Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Brisbane, QLD 4072, Australia; NHMRC Program in Membrane Interface Biology, Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Brisbane, QLD 4072, Australia; Centre for Microscopy and Microanalysis, The University of Queensland, St. Lucia, Brisbane, QLD 4072, Australia.
authors.forename: Robert G
authors.initials: RG
authors.lastname: Parton
authors.affiliation: Division of Cell Biology and Molecular Medicine, Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Brisbane, QLD 4072, Australia.
authors.forename: Guillermo A
authors.initials: GA
authors.lastname: Gomez
authors.affiliation: Division of Cell Biology and Molecular Medicine, Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Brisbane, QLD 4072, Australia; NHMRC Program in Membrane Interface Biology, Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Brisbane, QLD 4072, Australia. Electronic address: a.yap@uq.edu.au.
authors.forename: Alpha S
authors.initials: AS
authors.lastname: Yap
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D000199:Actins / Q000378:metabolism*; D022005:Adherens Junctions / Q000378:metabolism* / Q000502:physiology; D017209:Apoptosis / Q000502:physiology*; D018938:Caco-2 Cells; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000378:metabolism; D006801:Humans; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D009119:Muscle Contraction / Q000502:physiology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D000199:Actins; D015820:Cadherins; D008840:Microfilament Proteins; D034741:RNA, Small Interfering; C072061:coronin proteins
keywords: E-cadherin; actin organization; apoptosis; contractility; coronin