title: Does Circulating Antibody Play a Role in the Protection of Piglets against Porcine Epidemic Diarrhea Virus?
abstract: The contribution of circulating antibody to the protection of naïve piglets against porcine epidemic diarrhea virus (PEDV) was evaluated using a passive antibody transfer model. Piglets (n = 62) derived from 6 sows were assigned to one of 6 different treatments using a randomized block design which provided for allocation of all treatments to all sows' litters. Each treatment was designed to achieve a different level of circulating anti-PEDV antibody via intraperitoneally administration of concentrated serum antibody. Piglets were orally inoculated with PEDV (USA/IN/2013/19338E, 1 x 103 TCID50 per piglet) 24 hours later and then monitored for 14 days. Piglets remained with their dam throughout the experiment. Sow milk samples, piglet fecal samples, and data on piglet clinical signs, body weight, and body temperature were collected daily. Fecal samples were tested by PEDV real-time reverse transcriptase PCR. Serum, colostrum, and milk were tested for PEDV IgG, IgA, and virus-neutralizing antibody. The data were evaluated for the effects of systemic PEDV antibody levels on growth, body temperature, fecal shedding, survival, and antibody response. The analysis showed that circulating antibody partially ameliorated the effect of PEDV infection. Specifically, antibody-positive groups returned to normal body temperature faster and demonstrated a higher rate of survivability than piglets without PEDV antibody. When combined with previous literature on PEDV, it can be concluded that both systemic antibodies and maternal secretory IgA in milk contribute to the protection of the neonatal pig against PEDV infections. Overall, the results of this experiment suggested that passively administered circulating antibodies contributed to the protection of neonatal piglets against PEDV infection.
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, 50011, United States of America.
authors.forename: Korakrit
authors.initials: K
authors.lastname: Poonsuk
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, 50011, United States of America.
authors.forename: Luis Gabriel
authors.initials: LG
authors.lastname: Giménez-Lirola
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, 50011, United States of America.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, 50011, United States of America.
authors.forename: Paolo
authors.initials: P
authors.lastname: Arruda
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, 50011, United States of America.
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, 50011, United States of America.
authors.forename: Lucas
authors.initials: L
authors.lastname: Correa da Silva Carrion
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, 50011, United States of America.
authors.forename: Ronaldo
authors.initials: R
authors.lastname: Magtoto
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, 50011, United States of America.
authors.forename: Pablo
authors.initials: P
authors.lastname: Pineyro
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, 50011, United States of America.
authors.forename: Luciana
authors.initials: L
authors.lastname: Sarmento
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, 50011, United States of America.|Department of Statistics, College of Liberal Arts and Sciences, Iowa State University, Ames, Iowa, 50011, United States of America.
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, 50011, United States of America.|Department of Statistics, College of Liberal Arts and Sciences, Iowa State University, Ames, Iowa, 50011, United States of America.
authors.forename: Yaxuan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, 50011, United States of America.
authors.forename: Darin
authors.initials: D
authors.lastname: Madson
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, 50011, United States of America.
authors.forename: John
authors.initials: J
authors.lastname: Johnson
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, 50011, United States of America.
authors.forename: Kyoung-Jin
authors.initials: KJ
authors.lastname: Yoon
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, 50011, United States of America.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Zimmerman
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, 50011, United States of America.
authors.forename: Rodger
authors.initials: R
authors.lastname: Main
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D018352:Coronavirus Infections / Q000517:prevention & control* / Q000821:virology; D005243:Feces / Q000821:virology; D053485:Porcine epidemic diarrhea virus / Q000276:immunology*; D013552:Swine
chemical_list: D000914:Antibodies, Viral