title: Formation of gold decorated porphyrin nanoparticles and evaluation of their photothermal and photodynamic activity.
abstract: A core-shell gold (Au) nanoparticle with improved photosensitization have been successfully fabricated using Au nanoparticles and 5,10,15,20 tetrakis pentafluorophenyl)-21H,23H-porphine (PF6) dye, forming a dyad through molecular self-assembly. Au nanoparticles were decorated on the shell and PF6 was placed in the core of the nanoparticles. Highly stable Au nanoparticles were achieved using PF6 with poly(N-vinylcaprolactam-co-N-vinylimidazole)-g-poly(D,L-lactide) graft copolymer hybridization. This was compared with hybridization using cetyltrimethylammonium bromide and polyethylene glycol-b-poly(D,L-lactide) for shell formation with PF6-Au. The resulting PF6-poly(N-vinylcaprolactam-co-N-vinylimidazole)-g-poly(D,L-lactide)-Au core-shell nanoparticle were utilized for photothermal and photodynamic activities. The spectroscopic analysis and zeta potential values of micelles revealed the presence of a thin Au layer coated on the PF6 nanoparticle surface, which generally enhanced the thermal stability of the gold nanoparticles and the photothermal effect of the shell. The core-shell PF6-Au nanoparticles were avidly taken up by cells and demonstrated cellular phototoxicity upon irradiation with 300W halogen lamps. The structural arrangement of PF6 dyes in the core-shell particles assures the effectiveness of singlet oxygen production. The study verifies that PF6 particles when companied with Au nanoparticles as PF6-Au have possible combinational applications in photodynamic and photothermal therapies for cancer cells because of their high production of singlet oxygen and heat.
authors.affiliation: Division of Family Medicine, Department of Community Medicine, Taoyuan Armed Forces General Hospital, Taiwan, ROC.
authors.forename: Ruey-Juen
authors.initials: RJ
authors.lastname: Chen
authors.affiliation: Division of Family Medicine, Department of Community Medicine, Taoyuan Armed Forces General Hospital, Taiwan, ROC.
authors.forename: Po-Chung
authors.initials: PC
authors.lastname: Chen
authors.affiliation: Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 106, Taiwan, ROC.
authors.forename: Adhimoorthy
authors.initials: A
authors.lastname: Prasannan
authors.affiliation: Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 106, Taiwan, ROC.
authors.forename: Jayaraman
authors.initials: J
authors.lastname: Vinayagam
authors.affiliation: Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 106, Taiwan, ROC.
authors.forename: Chun-Chiang
authors.initials: CC
authors.lastname: Huang
authors.affiliation: Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 106, Taiwan, ROC.
authors.forename: Peng-Yi
authors.initials: PY
authors.lastname: Chou
authors.affiliation: Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 106, Taiwan, ROC.
authors.forename: Cheng-Chih
authors.initials: CC
authors.lastname: Weng
authors.affiliation: Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 106, Taiwan, ROC. Electronic address: h.c.tsai@mail.ntust.edu.tw.
authors.forename: Hsieh Chih
authors.initials: HC
authors.lastname: Tsai
authors.affiliation: National Applied Research Laboratories, Instrument Technology Research Center, Hsinchu 300, People's Republic of China.
authors.forename: Shuian-Yin
authors.initials: SY
authors.lastname: Lin
mesh_terms: D000072283:A549 Cells; D002470:Cell Survival / Q000187:drug effects; D000067493:Dynamic Light Scattering; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008175:Lung Neoplasms / Q000628:therapy; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D010778:Photochemotherapy; D010789:Phototherapy; D011108:Polymers / Q000737:chemistry; D011166:Porphyrins / Q000737:chemistry*; D026082:Singlet Oxygen / Q000378:metabolism; D013052:Spectrometry, X-Ray Emission; D014466:Ultraviolet Rays
chemical_list: D011108:Polymers; D011166:Porphyrins; D026082:Singlet Oxygen; D006046:Gold
keywords: Core–shell micelles; Gold nanoparticles; Nano composites; Photodynamic activity; Photothermal activity; Scanning electron microscopy