title: Tacky cyclic olefin copolymer: a biocompatible bonding technique for the fabrication of microfluidic channels in COC.
abstract: Cyclic olefin copolymer (COC) is widely used in microfluidics due to its UV-transparency, its biocompatibility and high chemical resistance. Here we present a fast and cost-effective solvent bonding technique, which allows for the efficient bonding of protein-patterned COC structures. The bonding process is carried out at room temperature and takes less than three minutes. Enzyme activity is retained upon bonding and microstructure deformation does not occur.
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute of Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany. bastian.rapp@kit.edu.
authors.forename: Nico
authors.initials: N
authors.lastname: Keller
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute of Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany. bastian.rapp@kit.edu.
authors.forename: Tobias M
authors.initials: TM
authors.lastname: Nargang
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute of Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany. bastian.rapp@kit.edu.
authors.forename: Matthias
authors.initials: M
authors.lastname: Runck
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute of Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany. bastian.rapp@kit.edu.
authors.forename: Frederik
authors.initials: F
authors.lastname: Kotz
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute of Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany. bastian.rapp@kit.edu.
authors.forename: Andreas
authors.initials: A
authors.lastname: Striegel
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute of Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany. bastian.rapp@kit.edu.
authors.forename: Kai
authors.initials: K
authors.lastname: Sachsenheimer
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute of Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany. bastian.rapp@kit.edu.
authors.forename: Denis
authors.initials: D
authors.lastname: Klemm
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute of Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany. bastian.rapp@kit.edu.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Länge
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute of Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany. bastian.rapp@kit.edu.
authors.forename: Matthias
authors.initials: M
authors.lastname: Worgull
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute of Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany. bastian.rapp@kit.edu.
authors.forename: Christiane
authors.initials: C
authors.lastname: Richter
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute of Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany. bastian.rapp@kit.edu.
authors.forename: Dorothea
authors.initials: D
authors.lastname: Helmer
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute of Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany. bastian.rapp@kit.edu.
authors.forename: Bastian E
authors.initials: BE
authors.lastname: Rapp
mesh_terms: D000096:Acetone / Q000737:chemistry; D000268:Adhesiveness; D001672:Biocompatible Materials / Q000737:chemistry*; D003510:Cyclohexanes / Q000737:chemistry; D003516:Cycloparaffins / Q000737:chemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D006536:Heptanes / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D056656:Lab-On-A-Chip Devices; D055616:Microtechnology / Q000379:methods*; D008954:Models, Biological; D012997:Solvents / Q000737:chemistry*; D013499:Surface Properties; D014050:Toluene / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D003510:Cyclohexanes; D003516:Cycloparaffins; D004800:Enzymes, Immobilized; D006536:Heptanes; D012997:Solvents; D000096:Acetone; D014050:Toluene; C028618:n-heptane; C506365:Cyclohexane; D006735:Horseradish Peroxidase