title: Methods for Assessing the In Vivo Role of PTEN in Glucose Homeostasis.
abstract: PTEN plays an important role in diabetes pathogenesis not only as a key negative regulator of the PI3K/Akt pathway required for insulin action, but also via its role in other cell processes required to maintain metabolic homeostasis. We describe the generation of tissue-specific PTEN knockout mice and models of both type 1 and type 2 diabetes, which we have found useful for the study of diabetes pathogenesis. We also outline common methods suitable for the characterization of glucose homeostasis in rodent models, including techniques to measure beta cell function and insulin sensitivity.
authors.affiliation: Toronto General Research Institute, University Health Network, Toronto, ON, Canada, M5G 2C4.|Institute of Medical Science, University of Toronto, Toronto, ON, Canada, M5G 2C4.
authors.forename: Cynthia T
authors.initials: CT
authors.lastname: Luk
authors.affiliation: Toronto General Research Institute, University Health Network, Toronto, ON, Canada, M5G 2C4.
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Schroer
authors.affiliation: Toronto General Research Institute, University Health Network, Toronto, ON, Canada, M5G 2C4. mwoo@uhnresearch.ca.|Institute of Medical Science, University of Toronto, Toronto, ON, Canada, M5G 2C4. mwoo@uhnresearch.ca.|Division of Endocrinology, Department of Medicine, University Health Network, University of Toronto, Toronto, ON, Canada, M5G 2C4. mwoo@uhnresearch.ca.|Toronto General Research Institute, 101 College Street, MaRS Centre/TMDT, Room 10-363, Toronto, ON, Canada, M5G 1L7. mwoo@uhnresearch.ca.
authors.forename: Minna
authors.initials: M
authors.lastname: Woo
mesh_terms: D000818:Animals; D018360:Crystallography, X-Ray; D003920:Diabetes Mellitus / Q000378:metabolism*; D004195:Disease Models, Animal; D005947:Glucose / Q000378:metabolism*; D006706:Homeostasis; D006801:Humans; D007328:Insulin / Q000378:metabolism; D051379:Mice; D051059:PTEN Phosphohydrolase / Q000378:metabolism*
chemical_list: D007328:Insulin; D051059:PTEN Phosphohydrolase; D005947:Glucose
keywords: Diabetes; Glucose; Insulin sensitivity; PTEN