title: The intestinal microbiota of piglets fed with wheat bran variants as characterised by 16S rRNA next-generation amplicon sequencing.
abstract: The intestinal microbiota of piglets fed with a Control diet low in dietary fibre and modified wheat bran variants as an additional source of insoluble dietary fibre was characterised. In this context, variances in the microbiota of three different gut segments were assessed. Wheat bran was either included in its native form or modified by fermentation and extrusion before added at 150 g/kg to a basal diet for 48 piglets (12 animals per treatment). Total DNA was extracted from digesta samples from the jejunum, the end of the ileum and the colon ascendens. Samples were prepared accordingly for subsequent sequencing with the Illumina MiSeq. The obtained results revealed distinct location-specific differences in microbial composition. While Firmicutes were most predominant in all three gut segments, Bacteroidetes were additionally found in the colon at high abundance. The parameters of alpha and beta diversity analysis showed significant differences (p < 0.01) between the colon and the other two gut segments. Specialised bacterial groups like Prevotella and Ruminococcaceae were among the most predominant ones found in the colon, as they possess cellulolytic properties to degrade (at least partially) non-starch polysaccharides, while their abundance was negligible in the jejunum and the ileum. Conversely, the genera Lactobacillus, Bifidobacterium and Veillonella, for example, were among the most predominant groups in the jejunum and ileum, while in the colon they were hardly found. Although statistical taxonomical evaluation, following p-value correction, did not reveal pronounced differences in abundance related to bran modification, alpha and beta diversity analysis showed an influence regarding the various feeding strategies applied. Based on these findings, a more in-depth view on intestinal microbial composition within the gastrointestinal tract of young pigs fed with low- and high-fibre diets was generated.
authors.affiliation: a Institute of Food Science, Department of Food Science and Technology , University of Natural Resources and Life Sciences , Vienna , Austria.|b Christian Doppler Research Laboratory for Innovative Bran Biorefinery, Department of Food Science and Technology , University of Natural Resources and Life Sciences , Vienna , Austria.
authors.forename: Manuel
authors.initials: M
authors.lastname: Kraler
authors.affiliation: a Institute of Food Science, Department of Food Science and Technology , University of Natural Resources and Life Sciences , Vienna , Austria.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Ghanbari
authors.affiliation: a Institute of Food Science, Department of Food Science and Technology , University of Natural Resources and Life Sciences , Vienna , Austria.
authors.forename: Konrad J
authors.initials: KJ
authors.lastname: Domig
authors.affiliation: c Institute of Animal Nutrition, Livestock Products, and Nutrition Physiology, Department of Agrobiotechnology , University of Natural Resources and Life Sciences , Vienna , Austria.
authors.forename: Karl
authors.initials: K
authors.lastname: Schedle
authors.affiliation: a Institute of Food Science, Department of Food Science and Technology , University of Natural Resources and Life Sciences , Vienna , Austria.|b Christian Doppler Research Laboratory for Innovative Bran Biorefinery, Department of Food Science and Technology , University of Natural Resources and Life Sciences , Vienna , Austria.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Kneifel
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D004032:Diet / Q000662:veterinary; D004043:Dietary Fiber / Q000172:deficiency / Q000378:metabolism*; D000069196:Gastrointestinal Microbiome; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D017422:Sequence Analysis, DNA / Q000662:veterinary; D034421:Sus scrofa / Q000382:microbiology* / Q000502:physiology*
chemical_list: D004043:Dietary Fiber; D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA; fibre; high-throughput sequencing; microbial ecology; pigs; wheat bran