title: The Functional Human C-Terminome.
abstract: All translated proteins end with a carboxylic acid commonly called the C-terminus. Many short functional sequences (minimotifs) are located on or immediately proximal to the C-terminus. However, information about the function of protein C-termini has not been consolidated into a single source. Here, we built a new "C-terminome" database and web system focused on human proteins. Approximately 3,600 C-termini in the human proteome have a minimotif with an established molecular function. To help evaluate the function of the remaining C-termini in the human proteome, we inferred minimotifs identified by experimentation in rodent cells, predicted minimotifs based upon consensus sequence matches, and predicted novel highly repetitive sequences in C-termini. Predictions can be ranked by enrichment scores or Gene Evolutionary Rate Profiling (GERP) scores, a measurement of evolutionary constraint. By searching for new anchored sequences on the last 10 amino acids of proteins in the human proteome with lengths between 3-10 residues and up to 5 degenerate positions in the consensus sequences, we have identified new consensus sequences that predict instances in the majority of human genes. All of this information is consolidated into a database that can be accessed through a C-terminome web system with search and browse functions for minimotifs and human proteins. A known consensus sequence-based predicted function is assigned to nearly half the proteins in the human proteome. Weblink: http://cterminome.bio-toolkit.com.
authors.affiliation: Nevada Institute of Personalized Medicine, and School of Life Sciences, University of Nevada, Las Vegas, Nevada, United States of America.
authors.forename: Surbhi
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Nevada Institute of Personalized Medicine, and School of Life Sciences, University of Nevada, Las Vegas, Nevada, United States of America.
authors.forename: Oniel
authors.initials: O
authors.lastname: Toledo
authors.affiliation: Nevada Institute of Personalized Medicine, and School of Life Sciences, University of Nevada, Las Vegas, Nevada, United States of America.
authors.forename: Michael
authors.initials: M
authors.lastname: Hedden
authors.affiliation: Nevada Institute of Personalized Medicine, and School of Life Sciences, University of Nevada, Las Vegas, Nevada, United States of America.
authors.forename: Kenneth F
authors.initials: KF
authors.lastname: Lyon
authors.affiliation: Nevada Institute of Personalized Medicine, and School of Life Sciences, University of Nevada, Las Vegas, Nevada, United States of America.
authors.forename: Steven B
authors.initials: SB
authors.lastname: Brooks
authors.affiliation: Nevada Institute of Personalized Medicine, and School of Life Sciences, University of Nevada, Las Vegas, Nevada, United States of America.
authors.forename: Roxanne P
authors.initials: RP
authors.lastname: David
authors.affiliation: Nevada Institute of Personalized Medicine, and School of Life Sciences, University of Nevada, Las Vegas, Nevada, United States of America.
authors.forename: Justin
authors.initials: J
authors.lastname: Limtong
authors.affiliation: Nevada Institute of Personalized Medicine, and School of Life Sciences, University of Nevada, Las Vegas, Nevada, United States of America.
authors.forename: Jacklyn M
authors.initials: JM
authors.lastname: Newsome
authors.affiliation: Nevada Institute of Personalized Medicine, and School of Life Sciences, University of Nevada, Las Vegas, Nevada, United States of America.
authors.forename: Nemanja
authors.initials: N
authors.lastname: Novakovic
authors.affiliation: Department of Computer Science and Engineering, University of Connecticut, Storrs, Connecticut 06269-2155, United States of America.
authors.forename: Sanguthevar
authors.initials: S
authors.lastname: Rajasekaran
authors.affiliation: Department of Pathology, Massachusetts General Hospital, Boston, Massachusetts 02114, United States of America.
authors.forename: Vishal
authors.initials: V
authors.lastname: Thapar
authors.affiliation: Nevada Institute of Personalized Medicine, and School of Life Sciences, University of Nevada, Las Vegas, Nevada, United States of America.
authors.forename: Sean R
authors.initials: SR
authors.lastname: Williams
authors.affiliation: Nevada Institute of Personalized Medicine, and School of Life Sciences, University of Nevada, Las Vegas, Nevada, United States of America.
authors.forename: Martin R
authors.initials: MR
authors.lastname: Schiller
mesh_terms: D000595:Amino Acid Sequence; D030562:Databases, Protein; D006801:Humans; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins