title: [MASS-SPECTROMETRY IN MICROBIOLOGICAL PRACTICE OF SCIENTIFIC CENTRE OF OBSTETRICS, GYNECOLOGY AND PERINATOLOGY].
abstract: OBJECTIVE
  Comparative evaluation of species identification of microorganisms by MALDI-TOF mass-spectrometry and automatic biochemical analyzer VITEK2 Compact 30.
  
  
  METHODS
  Species identification of 18,400 isolates of microorganisms (staphylococci, streptococci, enterococci, enterobacteria, nonfermenting gram-negative bacteria, lactobacilli, anaerobes, yeast fungi, neisseriae), isolated from vagina of pregnant and non-pregnant women and from newborns, was carried out. Identification of the isolated microorganisms was carried out by automatic bacteriologic analyzer VITEK2 Compact30 (BioMerieuX, France) and MALDI-TOF-MS analysis method on AutofleXIII (Bruker Daltonics, Germany) mass-spectrometer.
  
  
  RESULTS
  Comparative identification of 2005 isolates of microorganisms was carried out. Sequencing of ribosomal RNA was used as a reference method. Authenticity of species identification my MALDI-TOF-MS analysis method was: for staphylococci (95.8%), enterococci (97.5%), enterobacteria (98.4%), nonfermenting gram-negative bacteria (93.6%), β-hemolytiC staphylococci (93.8%), lactobacilli (92.8%), yeast fungi (99.9%).
  
  
  CONCLUSIONS
  Introduction of MALDI-TOF-MS analysis technology into practical work of microbiological laboratories exceeds previously used methods of microbiological testing in terms of speed; cost and authenticity of identification of a wide spectrum of microorganisms.
authors.forename: T V
authors.initials: TV
authors.lastname: Priputnevich
authors.forename: A R
authors.initials: AR
authors.lastname: Melkumyan
authors.forename: L A
authors.initials: LA
authors.lastname: Lyubasovskaya
authors.forename: V V
authors.initials: VV
authors.lastname: Muravieva
authors.forename: E N
authors.initials: EN
authors.lastname: Ilina
authors.forename: G T
authors.initials: GT
authors.lastname: Sukhikh
mesh_terms: D000046:Academic Medical Centers / Q000379:methods*; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques / Q000295:instrumentation*; D005260:Female; D005658:Fungi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006176:Gynecology; D006801:Humans; D007231:Infant, Newborn; D016533:Mycological Typing Techniques / Q000295:instrumentation*; D009774:Obstetrics; D010500:Perinatology; D011247:Pregnancy; D012335:RNA, Ribosomal / Q000235:genetics* / Q000302:isolation & purification; D017423:Sequence Analysis, RNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014621:Vagina / Q000382:microbiology
chemical_list: D012335:RNA, Ribosomal