title: MiR-1180 promoted the proliferation of hepatocellular carcinoma cells by repressing TNIP2 expression.
abstract: MicroRNAs (miRNAs) are short, non-coding RNAs with post-transcriptional regulatory function, playing crucial roles in cancer development and progression of hepatocellular carcinoma (HCC). Previous studies have indicated that miR-1180 was implicated in diverse biological processes. However, the underlying mechanism of miR-1180 in HCC has not been intensively investigated. In this study, we aimed to investigate the role of miR-1180 and its target genes in HCC. We found that miR-1180 expression was significantly increased in HCC cells and clinical tissues compared with their corresponding controls. Overexpression of miR-1180 promoted cell proliferation in HCC cell line HepG2. TNFAIP3 interacting protein 2 (TNIP2), a potential target gene of miR-1180, and were validated by a luciferase assay. Further studies revealed that miR-1180 regulated cell proliferation of HCC by directly suppressing TNIP2 expression and the knockdown of TNIP2 expression reversed the effect of miR-1180-in on HCC cell proliferation. In summary, our data indicated that miR-1180 might act as a tumor promoter by targeting TNIP2 during development of HCC.
authors.affiliation: Department of Hepatobiliary Surgery, Provincial Hospital Affiliated to Shandong University, East District, Jinan 250014, Shandong Province, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Hepatobiliary Surgery, Provincial Hospital Affiliated to Shandong University, East District, Jinan 250014, Shandong Province, China.
authors.forename: Hua-Qiang
authors.initials: HQ
authors.lastname: Zhu
authors.affiliation: Department of Hepatobiliary Surgery, Provincial Hospital Affiliated to Shandong University, East District, Jinan 250014, Shandong Province, China.
authors.forename: Chao-Qun
authors.initials: CQ
authors.lastname: Ma
authors.affiliation: Department of Hepatobiliary Surgery, Provincial Hospital Affiliated to Shandong University, East District, Jinan 250014, Shandong Province, China.
authors.forename: Hong-Guang
authors.initials: HG
authors.lastname: Li
authors.affiliation: Department of Hepatobiliary Surgery, Provincial Hospital Affiliated to Shandong University, East District, Jinan 250014, Shandong Province, China.
authors.forename: Fang-Feng
authors.initials: FF
authors.lastname: Liu
authors.affiliation: Department of Hepatobiliary Surgery, Provincial Hospital Affiliated to Shandong University, East District, Jinan 250014, Shandong Province, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Chang
authors.affiliation: Department of Hepatobiliary Surgery, Provincial Hospital Affiliated to Shandong University, East District, Jinan 250014, Shandong Province, China. Electronic address: junlushandong@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Lu
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology*; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D015854:Up-Regulation / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D048868:Adaptor Proteins, Signal Transducing; D035683:MicroRNAs; C434062:TNIP2 protein, human
keywords: Cell proliferation; Hepatocellular carcinoma; TNIP2; miR-1180