title: Chimeric Antigen Receptors for Cancer Immunotherapy.
abstract: The adoptive transfer of T lymphocytes expressing chimeric antigen receptors (CARs) has emerged as a promising treatment for various lymphoid and solid malignancies. Patients treated with CAR-T cells have achieved dramatic responses and in some cases, complete tumor eradication. Given that CARs combine the specificity of a monoclonal antibody with the internal signaling domains of T cells, there is flexibility for choice of target antigen and strength of T-cell activation. This targeting mechanism also relinquishes the need for antigen processing and presentation via the major histocompatibility complex (MHC), making CARs a very attractive therapeutic option for the majority of patients. This review describes current methodological strategies used to generate CAR molecules, how to insert these molecules in T lymphocytes and how to evaluate the functionality of these CAR-T cells using various in vitro and in vivo experiments.
authors.affiliation: Center for Cell and Gene Therapy, Baylor College of Medicine, 6621 Fannin St., MC 3-3320,, Houston, TX, 77030, USA.|Interdepartmental Program in Translational Biology and Molecular Medicine, Baylor College of Medicine, Houston, TX, USA.
authors.forename: Claudia
authors.initials: C
authors.lastname: Geldres
authors.affiliation: Center for Cell and Gene Therapy, Baylor College of Medicine, 6621 Fannin St., MC 3-3320,, Houston, TX, 77030, USA.|Department of Pediatrics, Baylor College of Medicine, Houston, TX, USA.|Texas Children's Hospital, Houston, TX, USA.
authors.forename: Barbara
authors.initials: B
authors.lastname: Savoldo
authors.affiliation: Center for Cell and Gene Therapy, Baylor College of Medicine, 6621 Fannin St., MC 3-3320,, Houston, TX, 77030, USA. gdotti@bcm.edu.|Interdepartmental Program in Translational Biology and Molecular Medicine, Baylor College of Medicine, Houston, TX, USA. gdotti@bcm.edu.|Texas Children's Hospital, Houston, TX, USA. gdotti@bcm.edu.|Departments of Pathology, Baylor College of Medicine, Houston, TX, USA. gdotti@bcm.edu.|Departments of Immunology, Baylor College of Medicine, Houston, TX, USA. gdotti@bcm.edu.|Department of Medicine, Baylor College of Medicine, Houston, TX, USA. gdotti@bcm.edu.|Houston Methodist Hospital, Houston, TX, USA. gdotti@bcm.edu.
authors.forename: Gianpietro
authors.initials: G
authors.lastname: Dotti
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D018920:Coculture Techniques; D057809:HEK293 Cells; D006801:Humans; D020014:K562 Cells; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000628:therapy*; D011946:Receptors, Antigen / Q000096:biosynthesis* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D011946:Receptors, Antigen; D011993:Recombinant Fusion Proteins
keywords: Chimeric antigen receptor; Immunotherapy