title: Th17 expression and IL-17 levels in laryngeal squamous cell carcinoma patients.
abstract: CONCLUSIONS
  The Th17 cell frequency in peripheral blood and levels of IL-17 showed significant differences between patients with laryngeal squamous cell carcinoma and those with vocal cords polyps. Serum levels of IL-17 were correlated with laryngocarcinoma staging.
  
  
  OBJECTIVE
  To investigate associations among the frequency of Th17 cells, levels of IL-17, and laryngeal squamous cell carcinoma.
  
  
  METHODS
  Eighty in-patients with laryngeal squamous cell carcinoma and 114 in-patients with polypus of the vocal cord were enrolled. Th17 cell frequencies in peripheral blood and serum levels of IL-17 were measured by flow cytometry and enzyme-linked immunosorbent assay, respectively. The tissue expression levels of IL-17 mRNA transcripts and protein were measured using quantitative RT-PCR or immunohistochemical detection, respectively.
  
  
  RESULTS
  Th17 cell frequencies in peripheral blood and serum concentrations of IL-17 were significantly higher in patients with laryngocarcinoma compared with those in patients with polyps (p < 0.01 for both Th17 cells and IL-17 levels). Serum concentrations of IL-17 were significantly higher in patients with advanced laryngocarcinoma than in patients with early laryngocarcinoma (p < 0.01). The mRNA and protein levels of IL-17 were significantly higher in laryngocarcinoma tissues than in adjacent normal tissues (p < 0.01 for mRNA levels, p < 0.05 for protein levels).
authors.affiliation: a Department of Otolaryngology , the Second Affiliated Hospital, School of Medicine, Zhejiang University , Hangzhou , PR China ;
authors.forename: Fu-Jun
authors.initials: FJ
authors.lastname: Li
authors.affiliation: b Institute of Immunology, School of Medicine, Zhejiang University , Hangzhou , PR China ;
authors.forename: Zhi-Jian
authors.initials: ZJ
authors.lastname: Cai
authors.affiliation: b Institute of Immunology, School of Medicine, Zhejiang University , Hangzhou , PR China ;
authors.forename: Fei
authors.initials: F
authors.lastname: Yang
authors.affiliation: c Department of Otolaryngology , Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University , Hangzhou , PR China.
authors.forename: Shou-De
authors.initials: SD
authors.lastname: Zhang
authors.affiliation: a Department of Otolaryngology , the Second Affiliated Hospital, School of Medicine, Zhejiang University , Hangzhou , PR China ;
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
mesh_terms: D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000276:immunology*; D016022:Case-Control Studies; D006801:Humans; D020381:Interleukin-17 / Q000097:blood*; D053759:Interleukin-23 / Q000097:blood; D007822:Laryngeal Neoplasms / Q000097:blood / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3 / Q000097:blood; D011127:Polyps / Q000097:blood / Q000276:immunology; D058504:Th17 Cells
chemical_list: D020381:Interleukin-17; D053759:Interleukin-23; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; C539900:RORC protein, human
keywords: Prognosis; T-helper 17 cells; head and neck cancers; immunity and tumorigenesis; interleukin-17