title: Altered expression of progesterone receptor isoforms A and B in human eutopic endometrium in endometriosis patients.
abstract: Recent data implicate an altered expression of progesterone receptor isoform A (PR-A) and B (PR-B) in the endometrium of endometriosis patients. This prospective exploratory study aimed to precisely determine the PR-A and PR-B expression using immunohistochemical techniques in eutopic endometrium of women with endometriosis compared with disease-free women throughout the menstrual cycle. All symptomatic patients underwent laparoscopy for the diagnosis of endometriosis and histological confirmation of the disease (EO) whereas controls were proven disease-free (CO). In CO samples (n=10) an increased expression of PR-A and PR-B during the proliferative to early secretory phase and a decreased expression of both receptor isoforms during the mid to late secretory phase was ascertained in accordance with previous studies. In patients with endometriosis (n=16) no cycle dependent pattern of PR-A and PR-B expression was identified in contrast to patients without endometriosis. Moreover, in EO samples a huge variety of inter- and intra-individual differences in PR-A and PR-B expression were detected. These data provide further evidence that dysregulation of the PR-A and PR-B expression might contribute to the pathophysiology of endometriosis.
authors.affiliation: Department of Obstetrics and Gynecology, Medical University of Graz, Auenbrugger Platz 14, 8036 Graz, Austria.
authors.forename: Monika Martina
authors.initials: MM
authors.lastname: Wölfler
authors.affiliation: Institute of Molecular and Cellular Anatomy, Medical Faculty of the RWTH Aachen University, Wendlingweg 2, 52074 Aachen, Germany.
authors.forename: Mareike
authors.initials: M
authors.lastname: Küppers
authors.affiliation: Department of Obstetrics and Gynecology, Medical Faculty of the RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany.
authors.forename: Werner
authors.initials: W
authors.lastname: Rath
authors.affiliation: Institute of Molecular and Cellular Anatomy, Medical Faculty of the RWTH Aachen University, Wendlingweg 2, 52074 Aachen, Germany.
authors.forename: Volker Uwe
authors.initials: VU
authors.lastname: Buck
authors.affiliation: Department of Obstetrics and Gynecology, Medical Faculty of the RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany.
authors.forename: Ivo
authors.initials: I
authors.lastname: Meinhold-Heerlein
authors.affiliation: Institute of Molecular and Cellular Anatomy, Medical Faculty of the RWTH Aachen University, Wendlingweg 2, 52074 Aachen, Germany. Electronic address: iclassen-linke@ukaachen.de.
authors.forename: Irmgard
authors.initials: I
authors.lastname: Classen-Linke
mesh_terms: D000293:Adolescent; D000328:Adult; D004715:Endometriosis / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008597:Menstrual Cycle / Q000378:metabolism*; D008875:Middle Aged; D011980:Receptors, Progesterone / Q000378:metabolism*; D014018:Tissue Distribution; D055815:Young Adult
chemical_list: D011980:Receptors, Progesterone; C101118:progesterone receptor A; C097314:progesterone receptor B
keywords: Endometriosis; Eutopic endometrium; Immunohistochemistry; Menstrual cycle; PR-A/PR-B