title: DNA Equation of State: In Vitro vs In Viro.
abstract: We formulate a continuum approach to the equation of state (density dependence of osmotic pressure) of bulk DNA and encapsidated DNA, as well as review the phase diagram of DNA in the regime of densities relevant for DNA packing in bacteriophages. We derive the first integral of the equilibrium equations that connects the behavior of DNA in the bulk and in nanoscale enclosures, and we delineate the changes wrought upon the mesophase equilibria of encapsidated DNA. We show how multiphase equilibria and complicated spatial distribution of DNA density and orientation can emerge due to the curvature contribution to the DNA osmotic pressure within the capsid.
authors.affiliation: Department of Physics, University of Massachusetts , Amherst, Massachusetts 01003, United States.|Department of Theoretical Physics, Jožef Stefan Institute , SI-1000 Ljubljana, Slovenia.|Department of Physics, Faculty of Mathematics and Physics, University of Ljubljana , SI-1000 Ljubljana, Slovenia.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Podgornik
authors.affiliation: Department of Physics, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: M Alphan
authors.initials: MA
authors.lastname: Aksoyoglu
authors.affiliation: Department of Physics, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: Selcuk
authors.initials: S
authors.lastname: Yasar
authors.affiliation: Department of Physics, Faculty of Mathematics and Physics, University of Ljubljana , SI-1000 Ljubljana, Slovenia.
authors.forename: Daniel
authors.initials: D
authors.lastname: Svenšek
authors.affiliation: Department of Physics, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: V Adrian
authors.initials: VA
authors.lastname: Parsegian
mesh_terms: D001435:Bacteriophages / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002213:Capsid / Q000737:chemistry / Q000378:metabolism; D004279:DNA, Viral / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009997:Osmotic Pressure; D012996:Solutions / Q000737:chemistry
chemical_list: D004279:DNA, Viral; D012996:Solutions