title: MicroRNA-202 inhibits tumor progression by targeting LAMA1 in esophageal squamous cell carcinoma.
abstract: Esophageal squamous cell carcinoma (ESCC) is one of the most aggressive malignancies in the gastrointestinal tract. Emerging studies have indicated that microRNAs (miRNAs) are strongly implicated in the development and progression of ESCC. Here, we focused on the function and the underlying molecular mechanism of miR-202 in ESCC. The results showed that miR-202 was significantly down-regulated in ESCC tissues and cell lines. Overexpression of miR-202 in ECa-109 and KYSE-510 cells markedly suppressed cell proliferation and cell migration, and induced cell apoptosis. Furthermore, laminin α1 (LAMA1) expression was frequently positive in ESCC tissues and inversely correlated with miR-202 expression. Then we demonstrated that miR-202 targeted 3'-untranslated region (UTR) of LAMA1 and inhibited its protein expression. Additionally, LAMA1 overexpression rescued the proliferation inhibition and cell apoptosis elevation induced by miR-202. MiR-202 also inhibited the protein expression of p-FAK and p-Akt, which were all reversed by LAMA1 overexpression. Taken together, these findings suggest that miR-202 may function as a novel tumor suppressor in ESCC by repressing cell proliferation and migration, and its biological effects may attribute the inhibition of LAMA1-mediated FAK-PI3K-Akt signaling.
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Zhengzhou University, 1 East Jianshe Road, Zhengzhou 450000, Henan Province, People's Republic of China. Electronic address: xiangruimengzz@163.com.
authors.forename: Xiangrui
authors.initials: X
authors.lastname: Meng
authors.affiliation: Department of Digestion and Oncology, The First Affiliated Hospital of Henan Uninversity of TCM, 19 Renmin Road, Zhengzhou 450000, Henan Province, People's Republic of China.
authors.forename: Xiaoqi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Gastrointestinal Surgery, The People's Hospital of Zhengzhou, 33 Huanghe Road, Zhengzhou 450000, Henan Province, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Lu
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Zhengzhou University, 1 East Jianshe Road, Zhengzhou 450000, Henan Province, People's Republic of China.
authors.forename: Wang
authors.initials: W
authors.lastname: Ma
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Zhengzhou University, 1 East Jianshe Road, Zhengzhou 450000, Henan Province, People's Republic of China.
authors.forename: Dongli
authors.initials: D
authors.lastname: Yue
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Zhengzhou University, 1 East Jianshe Road, Zhengzhou 450000, Henan Province, People's Republic of China.
authors.forename: Lijie
authors.initials: L
authors.lastname: Song
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Zhengzhou University, 1 East Jianshe Road, Zhengzhou 450000, Henan Province, People's Republic of China.
authors.forename: Qingxia
authors.initials: Q
authors.lastname: Fan
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology*; D049109:Cell Proliferation / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology*; D000077277:Esophageal Squamous Cell Carcinoma; D016147:Genes, Tumor Suppressor; D006801:Humans; D007797:Laminin / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics
chemical_list: D007797:Laminin; C569392:MIRN202 microRNA, human; D035683:MicroRNAs; C084098:laminin A
keywords: Cell growth; Esophageal squamous cell carcinoma; FAK-PI3K-Akt signaling; Laminin α1; MiR-202