title: Intracellular Distribution of Capsid-Associated pUL77 of Human Cytomegalovirus and Interactions with Packaging Proteins and pUL93.
abstract: DNA packaging into procapsids is a common multistep process during viral maturation in herpesviruses. In human cytomegalovirus (HCMV), the proteins involved in this process are terminase subunits pUL56 and pUL89, which are responsible for site-specific cleavage and insertion of the DNA into the procapsid via portal protein pUL104. However, additional viral proteins are required for the DNA packaging process. We have shown previously that the plasmid that encodes capsid-associated pUL77 encodes another potential player during capsid maturation. Pulse-chase experiments revealed that pUL77 is stably expressed during HCMV infection. Time course analysis demonstrated that pUL77 is expressed in the early late part of the infectious cycle. The sequence of pUL77 was analyzed to find nuclear localization sequences (NLSs), revealing monopartite NLSm at the N terminus and bipartite NLSb in the middle of pUL77. The potential NLSs were inserted into plasmid pHM829, which encodes a chimeric protein with β-galactosidase and green fluorescent protein. In contrast to pUL56, neither NLSm nor NLSb was sufficient for nuclear import. Furthermore, we investigated by coimmunoprecipitation whether packaging proteins, as well as pUL93, the homologue protein of herpes simplex virus 1 pUL17, are interaction partners of pUL77. The interactions between pUL77 and packaging proteins, as well as pUL93, were verified.
  
  
  
  We showed that the capsid-associated pUL77 is another potential player during capsid maturation of HCMV. Protein UL77 (pUL77) is a conserved core protein of HCMV. This study demonstrates for the first time that pUL77 has early-late expression kinetics during the infectious cycle and an intrinsic potential for nuclear translocation. According to its proposed functions in stabilization of the capsid and anchoring of the encapsidated DNA during packaging, interaction with further DNA packaging proteins is required. We identified physical interactions with terminase subunits pUL56 and pUL89 and another postulated packaging protein, pUL93, in infected, as well as transfected, cells.
authors.affiliation: Institute of Medical Virology, Charité Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: Pánja
authors.initials: P
authors.lastname: Köppen-Rung
authors.affiliation: Institute of Medical Virology, Charité Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Dittmer
authors.affiliation: Institute of Medical Virology, Charité Universitätsmedizin Berlin, Berlin, Germany elke.bogner@charite.de.
authors.forename: Elke
authors.initials: E
authors.lastname: Bogner
mesh_terms: D002213:Capsid / Q000737:chemistry / Q000378:metabolism*; D036022:Capsid Proteins / Q000378:metabolism*; D003587:Cytomegalovirus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D003593:Cytoplasm / Q000737:chemistry* / Q000378:metabolism / Q000821:virology; D042003:DNA Packaging; D004279:DNA, Viral / Q000235:genetics; D004706:Endodeoxyribonucleases / Q000378:metabolism; D005347:Fibroblasts / Q000821:virology; D049452:Green Fluorescent Proteins / Q000235:genetics; D057809:HEK293 Cells; D018259:Herpesvirus 1, Human / Q000235:genetics; D006801:Humans; D019913:Nuclear Localization Signals / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism*; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral; D019913:Nuclear Localization Signals; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D049452:Green Fluorescent Proteins; D004706:Endodeoxyribonucleases; C030894:terminase; D001616:beta-Galactosidase