title: Comparison of P75 NTR-positive and -negative etcomesenchymal stem cell odontogenic differentiation through epithelial-mesenchymal interaction.
abstract: OBJECTIVE
  The aim of this study was to investigate differences of odonto-differentiation between P75 -neurotrophin receptor (P75 -NTR)-positive ectomesenchymal stem cells (P75+EMSCs) and P75 -NTR-negative ectomesenchymal stem cells (P75-EMSCs), and their underlying mechanisms.
  
  
  METHODS
  Primary cranial neural crest-derived cells (CNC) were isolated from the first branchial arches, and P75+EMSCs and P75-EMSCs were sorted by fluorescence-activated cell sorting. Differentiation of P75+EMSCs or P75-EMSCs into odontoblast-like cells was induced by dental epithelial cells in vitro or in vivo. Differential gene expression profiles between P75+EMSCs and P75-EMSCs were analysed by microarray assay. Smad4-specific small interfering RNA and activator kartogenin were used to treat the cells, to evaluate effects of Smad4 in odonto-differentiation of P75+EMSCs or P75-EMSCs.
  
  
  RESULTS
  Under induction of dental epithelium conditioned medium, P75+EMSCs had more mineralized node formation and higher expression of Dmp1 and Dspp compared to P75-EMSCs. In our in vivo study, graft of P75+EMSCs recombination with dental epithelium showed higher expression of DMP1 and DSP. Knock-down of Smad4 in P75+EMSCs significantly downregulated expression of DMP1 and DSP, while activation of Smad4 in P75-EMSCs by the activator kartogenin, significantly increased DSP and DMP1 expression.
  
  
  CONCLUSIONS
  P75+EMSCs showed more odonto-differentiation potential than P75-EMSCs both in vivo and in vitro. Smad4 played a critical role in determination of odonto-differentiation potential of CNC-derived EMSCs.
authors.affiliation: Department of Stomatology, Daping Hospital & Research Institute of Surgery, Third Military Medical University, Chongqing, 400038, China.|Affiliated Stomatological Hospital, PLA General Hospital of Chengdu Military Region, Chengdu, 610083, China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Xing
authors.affiliation: Department of Stomatology, Daping Hospital & Research Institute of Surgery, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Nie
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.|National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of Stomatology, Daping Hospital & Research Institute of Surgery, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Xiujie
authors.initials: X
authors.lastname: Wen
authors.affiliation: Department of Stomatology, Daping Hospital & Research Institute of Surgery, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Stomatology, Daping Hospital & Research Institute of Surgery, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.|National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Tian
authors.affiliation: Department of Stomatology, Daping Hospital & Research Institute of Surgery, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Luchuan
authors.initials: L
authors.lastname: Liu
mesh_terms: D000813:Anilides / Q000494:pharmacology; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D051180:Desmoplakins / Q000096:biosynthesis; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D009432:Neural Crest / Q000196:embryology; D054259:Neural Tube / Q000196:embryology; D009804:Odontoblasts / Q000166:cytology*; D009805:Odontogenesis / Q000502:physiology*; D010750:Phosphoproteins / Q000096:biosynthesis; D010795:Phthalic Acids / Q000494:pharmacology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D020800:Receptor, Nerve Growth Factor / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051901:Smad4 Protein / Q000235:genetics*
chemical_list: D000813:Anilides; D051180:Desmoplakins; C081635:Dmp1 protein, rat; D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D010795:Phthalic Acids; D034741:RNA, Small Interfering; D020800:Receptor, Nerve Growth Factor; D051901:Smad4 Protein; C490811:Smad4 protein, rat; C572342:kartogenin