title: Assessing PTEN Subcellular Localization.
abstract: PTEN subcellular localization is fundamental in the execution of the distinct PTEN biological activities, including not only its PI(3,4,5)P3 phosphatase activity when associated to membranes but also its subcellular compartment-specific interactions with regulatory and effector proteins, including those exerted in the nucleus. As a consequence, PTEN subcellular localization is tightly regulated in vivo by both intrinsic and extrinsic mechanisms. The plasma membrane/nucleus/cytoplasm partitioning of PTEN has been the focus of several studies, both from a mechanistic and from a disease-association point of view. Here, we summarize the current knowledge on PTEN plasma membrane/nucleus/cytoplasm distribution, and present subcellular fractionation, immunofluorescence, and immunohistochemical methods to study the distribution and shuttling of PTEN between these subcellular compartments.
authors.affiliation: Centro de Investigación Príncipe Felipe, Valencia, 46013, Spain.|Department of Hematology and Medical Oncology, Biomedical Research Institute INCLIVA, Valencia, 46010, Spain.
authors.forename: Anabel
authors.initials: A
authors.lastname: Gil
authors.affiliation: Department of Pathology, Cruces University Hospital, University of the Basque Country (UPV/EHU), Barakaldo, 48903, Spain.|Biocruces Health Research Institute, Plaza de Cruces s/n, Barakaldo, Bizkaia, 48903, Spain.
authors.forename: José I
authors.initials: JI
authors.lastname: López
authors.affiliation: Centro de Investigación Príncipe Felipe, Valencia, 46013, Spain. rpulidomurillo@gmail.com.|Biocruces Health Research Institute, Plaza de Cruces s/n, Barakaldo, Bizkaia, 48903, Spain. rpulidomurillo@gmail.com.|IKERBASQUE, Basque Foundation for Science, Bilbao, 48013, Spain. rpulidomurillo@gmail.com.
authors.forename: Rafael
authors.initials: R
authors.lastname: Pulido
mesh_terms: D000818:Animals; D002460:Cell Line; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D051059:PTEN Phosphohydrolase / Q000032:analysis*
chemical_list: D051059:PTEN Phosphohydrolase
keywords: Immunofluorescence; Immunohistochemistry; PTEN; Subcellular fractionation; Tumor suppression