title: Selenium inhibits Staphylococcus aureus-induced inflammation by suppressing the activation of the NF-κB and MAPK signalling pathways in RAW264.7 macrophages.
abstract: Inflammation is the hallmark of Staphylococcus aureus (S. aureus)-induced mastitis. Given the interesting relationship between selenium levels and inflammation, this study aimed to demonstrate that selenium modulated the inflammation reaction by suppressing the nuclear factor kappa B (NF-κB) and mitogen activated protein kinase (MAPK) signalling pathways. RAW264.7 macrophages were treated with three different concentrations (1μmol/l, 1.5μmol/l, and 2μmol/l) of Na2SeO3 for 12h before infection with S. aureus for 6h, 8h, and 10h. The results showed that selenium significantly reduced the mRNA expression levels of tumour necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6). Furthermore, the release of TNF-α, IL-1β, and IL-6 was decreased significantly with selenium supplementation. In addition, selenium influenced the NF-κB signalling pathway by suppressing the activation of NF-κB p65 and degradation of inhibitory kappa-B (IκB). Selenium also suppressed extracellular regulated protein kinase (Erk), c-Jun N-terminal kinase (Jnk), and p38 phosphorylation through the MAPK signalling pathway. In conclusion, selenium played an anti-inflammation role in RAW264.7 macrophages infected with S. aureus by suppressing the activation of the NF-κB and MAPK signalling pathways.
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious and Zoonoses, Yangzhou 225009, China.
authors.forename: Chong-Liang
authors.initials: CL
authors.lastname: Bi
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious and Zoonoses, Yangzhou 225009, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious and Zoonoses, Yangzhou 225009, China.
authors.forename: Yin-Jie
authors.initials: YJ
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious and Zoonoses, Yangzhou 225009, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Sun
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious and Zoonoses, Yangzhou 225009, China.
authors.forename: Jun-Sheng
authors.initials: JS
authors.lastname: Dong
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious and Zoonoses, Yangzhou 225009, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Meng
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious and Zoonoses, Yangzhou 225009, China. Electronic address: yzjjli@163.com.
authors.forename: Jian-Ji
authors.initials: JJ
authors.lastname: Li
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D007249:Inflammation / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D000067996:RAW 264.7 Cells; D012643:Selenium / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000502:physiology*
chemical_list: D016207:Cytokines; D016328:NF-kappa B; D012643:Selenium
keywords: Inflammation; MAPK; NF-κB; Selenium; Staphylococcus aureus; The chemical compounds that were used in this study were: selenium (PubChem CID: 6326970); diethyl pyrocarbonate (PubChem CID: 3051); dimethyl sulfoxide (PubChem CID: 679); ebselen (PubChem CID: 3194); l-glutamine (PubChem CID: 5961); sodium dodecyl sulphate (PubChem CID: 3423265); tween 20 (PubChem CID: 443314)