title: The effect of amifostine on bacterial translocation after radiation ınduced acute enteritis.
abstract: OBJECTIVE
  To investigate the effects of amifostine on bacterial translocation and overgrowth in colonic flora after acute radiation enteritis in a rat model.
  
  
  METHODS
  Thirty-two female Wistar albino rats were divided into four groups: Group-1 (n=8): only normal saline was administered intraperitoneally. Group-2 (n=8): first serum saline was administered intraperitoneally and 30 minutes later 20 Gy radiation was applied to abdominopelvic region. Group-3 (n=8): only amifostine 200 ml/kg was administered intraperitoneally and radiation was not applied. Group-4 (n=8): first amifostine 200 ml/kg was administered intraperitoneally and 30 minutes later 20 Gy radiation was applied to abdominopelvic region. On the 5th day after radiation, samples of mesenteric lymph tissues and cecal contents were taken by laparotomy for microbiological culture.
  
  
  RESULTS
  Intraperitoneal amifostine administration significantly decreased the bacterial overgrowth related to radiation in colon but did not significantly decrease the bacterial translocation. CONCLUSİON: Although not providing a full protection on the damaged mucosal barrier, amifostine significantly decreased the bacterial overgrowth in the cecal content after high dose radiation. There is a need to find out appropriate amifostine dose under different radiation applications avoiding bacterial translocation in gastrointestinal system.
authors.affiliation: Department of General Surgery, Faculty of Medicine, Çanakkale Onsekiz Mart University, Çanakkale, Turkey.
authors.forename: Sukru
authors.initials: S
authors.lastname: Tas
authors.affiliation: Department of General Surgery, Faculty of Medicine, Çanakkale Onsekiz Mart University, Çanakkale, Turkey.
authors.forename: Faruk
authors.initials: F
authors.lastname: Ozkul
authors.affiliation: Department of General Surgery, Faculty of Medicine, Çanakkale Onsekiz Mart University, Çanakkale, Turkey.
authors.forename: Muhammet Kasim
authors.initials: MK
authors.lastname: Arik
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Çanakkale Onsekiz Mart University, Çanakkale, Turkey.
authors.forename: Asli
authors.initials: A
authors.lastname: Kiraz
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Çanakkale Onsekiz Mart University, Çanakkale, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Vural
mesh_terms: D004999:Amifostine / Q000494:pharmacology*; D000818:Animals; D018988:Bacterial Translocation / Q000187:drug effects*; D002432:Cecum / Q000382:microbiology / Q000528:radiation effects; D004751:Enteritis / Q000139:chemically induced* / Q000382:microbiology / Q000517:prevention & control; D004755:Enterobacteriaceae / Q000502:physiology / Q000528:radiation effects*; D005260:Female; D008196:Lymph / Q000382:microbiology; D011829:Radiation Dosage; D011833:Radiation Injuries, Experimental / Q000382:microbiology* / Q000517:prevention & control; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D017208:Rats, Wistar
chemical_list: D011837:Radiation-Protective Agents; D004999:Amifostine