title: Bioenergetic relevance of hydrogen sulfide and the interplay between gasotransmitters at human cystathionine β-synthase.
abstract: Merely considered as a toxic gas in the past, hydrogen sulfide (H2S) is currently viewed as the third 'gasotransmitter' in addition to nitric oxide (NO) and carbon monoxide (CO), playing a key signalling role in human (patho)physiology. H2S can either act as a substrate or, similarly to CO and NO, an inhibitor of mitochondrial respiration, in the latter case by targeting cytochrome c oxidase (CcOX). The impact of H(2)S on mitochondrial energy metabolism crucially depends on the bioavailability of this gaseous molecule and its interplay with the other two gasotransmitters. The H(2)S-producing human enzyme cystathionine β-synthase (CBS), sustaining cellular bioenergetics in colorectal cancer cells, plays a role in the interplay between gasotransmitters. The enzyme was indeed recently shown to be negatively modulated by physiological concentrations of CO and NO, particularly in the presence of its allosteric activator S-adenosyl-l-methionine (AdoMet). These newly discovered regulatory mechanisms are herein reviewed. This article is part of a Special Issue entitled 'EBEC 2016: 19th European Bioenergetics Conference, Riva del Garda, Italy, July 2-6, 2016', edited by Prof. Paolo Bernardi.
authors.affiliation: Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República (EAN), 2780-156 Oeiras, Portugal. Electronic address: jvicente@itqb.unl.pt.
authors.forename: João B
authors.initials: JB
authors.lastname: Vicente
authors.affiliation: Department of Biochemical Sciences and Istituto Pasteur-Fondazione Cenci Bolognetti, Sapienza University of Rome, Piazzale Aldo Moro 5, I-00185 Rome, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Malagrinò
authors.affiliation: Department of Biochemical Sciences and Istituto Pasteur-Fondazione Cenci Bolognetti, Sapienza University of Rome, Piazzale Aldo Moro 5, I-00185 Rome, Italy.
authors.forename: Marzia
authors.initials: M
authors.lastname: Arese
authors.affiliation: Department of Biochemical Sciences and Istituto Pasteur-Fondazione Cenci Bolognetti, Sapienza University of Rome, Piazzale Aldo Moro 5, I-00185 Rome, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Forte
authors.affiliation: Department of Biochemical Sciences and Istituto Pasteur-Fondazione Cenci Bolognetti, Sapienza University of Rome, Piazzale Aldo Moro 5, I-00185 Rome, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Sarti
authors.affiliation: CNR Institute of Molecular Biology and Pathology, Piazzale Aldo Moro 5, I-00185 Rome, Italy. Electronic address: alessandro.giuffre@uniroma1.it.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Giuffrè
mesh_terms: D002248:Carbon Monoxide / Q000378:metabolism; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D003541:Cystathionine beta-Synthase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism; D064426:Gasotransmitters / Q000378:metabolism*; D015870:Gene Expression; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D006862:Hydrogen Sulfide / Q000378:metabolism*; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism* / Q000473:pathology; D008958:Models, Molecular; D009569:Nitric Oxide / Q000378:metabolism; D010085:Oxidative Phosphorylation; D012436:S-Adenosylmethionine / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D064426:Gasotransmitters; D009569:Nitric Oxide; D012436:S-Adenosylmethionine; D002248:Carbon Monoxide; D003576:Electron Transport Complex IV; D003541:Cystathionine beta-Synthase; D005978:Glutathione; D006862:Hydrogen Sulfide
keywords: Cystathionine β-synthase; Cytochrome c oxidase; Gasotransmitters; Heme chemistry; Mitochondrial respiration; Sulfide metabolism