title: The CDK-APC/C Oscillator Predominantly Entrains Periodic Cell-Cycle Transcription.
abstract: Throughout cell-cycle progression, the expression of multiple transcripts oscillate, and whether these are under the centralized control of the CDK-APC/C proteins or can be driven by a de-centralized transcription factor (TF) cascade is a fundamental question for understanding cell-cycle regulation. In budding yeast, we find that the transcription of nearly all genes, as assessed by RNA-seq or fluorescence microscopy in single cells, is dictated by CDK-APC/C. Three exceptional genes are transcribed in a pulsatile pattern in a variety of CDK-APC/C arrests. Pursuing one of these transcripts, the SIC1 inhibitor of B-type cyclins, we use a combination of mathematical modeling and experimentation to provide evidence that, counter-intuitively, Sic1 provides a failsafe mechanism promoting nuclear division when levels of mitotic cyclins are low.
authors.affiliation: Laboratory of Cell Cycle Genetics, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA; Center for Studies in Physics and Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA. Electronic address: sjrahi@rockefeller.edu.
authors.forename: Sahand Jamal
authors.initials: SJ
authors.lastname: Rahi
authors.affiliation: Laboratory of Cell Cycle Genetics, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA.
authors.forename: Kresti
authors.initials: K
authors.lastname: Pecani
authors.affiliation: Laboratory of Cell Cycle Genetics, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA.
authors.forename: Andrej
authors.initials: A
authors.lastname: Ondracka
authors.affiliation: Laboratory of Cell Cycle Genetics, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA; California Institute of Technology, 1200 E. California Boulevard, Pasadena, CA 91107, USA.
authors.forename: Catherine
authors.initials: C
authors.lastname: Oikonomou
authors.affiliation: Laboratory of Cell Cycle Genetics, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA; Center for Studies in Physics and Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA.
authors.forename: Frederick R
authors.initials: FR
authors.lastname: Cross
mesh_terms: D001683:Biological Clocks; D002453:Cell Cycle; D016213:Cyclins / Q000378:metabolism; D004398:Dyneins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D059010:Single-Cell Analysis; D014158:Transcription, Genetic
chemical_list: D016213:Cyclins; D029701:Saccharomyces cerevisiae Proteins; D004398:Dyneins; C083745:SLC1 protein, S cerevisiae