title: Anti-Apoptotic and Pro-Survival Effect of Alpinate Oxyphyllae Fructus (AOF) in a d-Galactose-Induced Aging Heart.
abstract: Aging, a natural biological/physiological phenomenon, is accelerated by reactive oxygen species (ROS) accumulation and identified by a progressive decrease in physiological function. Several studies have shown a positive relationship between aging and chronic heart failure (HF). Cardiac apoptosis was found in age-related diseases. We used a traditional Chinese medicine, Alpinate Oxyphyllae Fructus (AOF), to evaluate its effect on cardiac anti-apoptosis and pro-survival. Male eight-week-old Sprague-Dawley (SD) rats were segregated into five groups: normal control group (NC), d-Galactose-Induced aging group (Aging), and AOF of 50 (AL (AOF low)), 100 (AM (AOF medium)), 150 (AH (AOF high)) mg/kg/day. After eight weeks, hearts were measured by an Hematoxylin-Eosin (H&amp;E) stain, Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-assays and Western blotting. The experimental results show that the cardiomyocyte apoptotic pathway protein expression increased in the d-Galactose-Induced aging groups, with dose-dependent inhibition in the AOF treatment group (AL, AM, and AH). Moreover, the expression of the pro-survival p-Akt (protein kinase B (Akt)), Bcl-2 (B-cell lymphoma 2), anti-apoptotic protein (Bcl-xL) protein decreased significantly in the d-Galactose-induced aging group, with increased performance in the AOF treatment group with levels of p-IGFIR and p-PI3K (Phosphatidylinositol-3' kinase (PI3K)) to increase by dosage and compensatory performance. On the other hand, the protein of the Sirtuin 1 (SIRT1) pathway expression decreased in the aging groups and showed improvement in the AOF treatment group. Our results suggest that AOF strongly works against ROS-induced aging heart problems.
authors.affiliation: The School of Chinese Medicine for Post-Baccalaureate, I-Shou University, Kaohsiung 84001, Taiwan. dnas5728@yahoo.com.tw.|Chinese Medicine Department, E-DA Hospital, Kaohsiung 82445, Taiwan. dnas5728@yahoo.com.tw.|1PT Biotechnology Co., Ltd., Taichung 433, Taiwan. dnas5728@yahoo.com.tw.
authors.forename: Yung-Ming
authors.initials: YM
authors.lastname: Chang
authors.affiliation: Research Center for Chinese Medicine &amp; Acupuncture, China Medical University, Taichung 40402, Taiwan. glactose@hotmail.com.|Departments of Chinese Medicine, China Medical University Hospital, Taichung 40447, Taiwan. glactose@hotmail.com.|School of Post-Baccalaureate Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan. glactose@hotmail.com.
authors.forename: Hen-Hong
authors.initials: HH
authors.lastname: Chang
authors.affiliation: Department of Biological Science and Technology, China Medical University, Taichung 40447, Taiwan. wwkuo@mail.cmu.edu.tw.
authors.forename: Wei-Wen
authors.initials: WW
authors.lastname: Kuo
authors.affiliation: Departments of Chinese Medicine, China Medical University Hospital, Taichung 40447, Taiwan. kawabunga0809@yahoo.com.tw.|School of Post-Baccalaureate Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan. kawabunga0809@yahoo.com.tw.
authors.forename: Hung-Jen
authors.initials: HJ
authors.lastname: Lin
authors.affiliation: Department of pathology, Changhua Christian Hospital, Changhua 50506, Taiwan. 1867@cch.org.tw.|Department of Medical Technology, Jen-Teh Junior College of Medicine, Nursing and Management, Miaoli 35665, Taiwan. 1867@cch.org.tw.
authors.forename: Yu-Lan
authors.initials: YL
authors.lastname: Yeh
authors.affiliation: Department of Biotechnology, Bharathiar University, Coimbatore 641046, India. padma.vijaya@gmail.com.
authors.forename: Vijaya
authors.initials: V
authors.lastname: Padma Viswanadha
authors.affiliation: The School of Chinese Medicine for Post-Baccalaureate, I-Shou University, Kaohsiung 84001, Taiwan. gimmy09200430@yahoo.com.tw.|Chinese Medicine Department, E-DA Hospital, Kaohsiung 82445, Taiwan. gimmy09200430@yahoo.com.tw.
authors.forename: Chin-Chuan
authors.initials: CC
authors.lastname: Tsai
authors.affiliation: Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11042, Taiwan. rayjchen@tmu.edu.tw.
authors.forename: Ray-Jade
authors.initials: RJ
authors.lastname: Chen
authors.affiliation: Graduate Institute of Basic Medical Science, China Medical University, Taichung 40402, Taiwan. dadalidada0809@gmail.com.
authors.forename: Hsin-Nung
authors.initials: HN
authors.lastname: Chang
authors.affiliation: Graduate Institute of Basic Medical Science, China Medical University, Taichung 40402, Taiwan. cyhuang@mail.cmu.edu.tw.|School of Chinese Medicine, China Medical University, Taichung 40447, Taiwan. cyhuang@mail.cmu.edu.tw.|Department of Health and Nutrition Biotechnology, Asia University, Taichung 41354, Taiwan. cyhuang@mail.cmu.edu.tw.
authors.forename: Chih-Yang
authors.initials: CY
authors.lastname: Huang
mesh_terms: D000375:Aging / Q000187:drug effects*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D004452:Echocardiography; D005638:Fruit / Q000737:chemistry / Q000378:metabolism; D005690:Galactose / Q000494:pharmacology; D007150:Immunohistochemistry; D008297:Male; D008516:Medicine, Chinese Traditional; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D056564:Sirtuin 1 / Q000378:metabolism; D030521:Zingiberaceae / Q000737:chemistry* / Q000378:metabolism; D051020:bcl-X Protein / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D017382:Reactive Oxygen Species; D051020:bcl-X Protein; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D056564:Sirtuin 1; D005690:Galactose
keywords: ">d-galactose-induced aging; AOF; SIRT1; apoptosis