title: Single nucleotide polymorphism mining and nucleotide sequence analysis of Mx1 gene in exonic regions of Japanese quail.
abstract: OBJECTIVE
  An attempt has been made to study the Myxovirus resistant (Mx1) gene polymorphism in Japanese quail.
  
  
  METHODS
  In the present, investigation four fragments viz. Fragment I of 185 bp (Exon 3 region), Fragment II of 148 bp (Exon 5 region), Fragment III of 161 bp (Exon 7 region), and Fragment IV of 176 bp (Exon 13 region) of Mx1 gene were amplified and screened for polymorphism by polymerase chain reaction-single-strand conformation polymorphism technique in 170 Japanese quail birds.
  
  
  RESULTS
  Out of the four fragments, one fragment (Fragment II) was found to be polymorphic. Remaining three fragments (Fragment I, III, and IV) were found to be monomorphic which was confirmed by custom sequencing. Overall nucleotide sequence analysis of Mx1 gene of Japanese quail showed 100% homology with common quail and more than 80% homology with reported sequence of chicken breeds.
  
  
  CONCLUSIONS
  The Mx1 gene is mostly conserved in Japanese quail. There is an urgent need of comprehensive analysis of other regions of Mx1 gene along with its possible association with the traits of economic importance in Japanese quail.
authors.affiliation: Animal Genetics Division, Indian Veterinary Research Institute, Izatnagar, Bareilly - 243 122, Uttar Pradesh, India.
authors.forename: Diwesh Kumar
authors.initials: DK
authors.lastname: Niraj
authors.affiliation: Animal Genetics Division, Indian Veterinary Research Institute, Izatnagar, Bareilly - 243 122, Uttar Pradesh, India.
authors.forename: Pushpendra
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Department of Animal Genetics and Breeding, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha, India.
authors.forename: Chinmoy
authors.initials: C
authors.lastname: Mishra
authors.affiliation: Department of Avian Genetics and Breeding, Central Avian Research Institute, Izatnagar, Bareilly, 243 122, Uttar Pradesh, India.
authors.forename: Raj
authors.initials: R
authors.lastname: Narayan
authors.affiliation: Directorate of Poultry Research, Rajendranagar, Hyderabad, Telangana, India.
authors.forename: Tarun Kumar
authors.initials: TK
authors.lastname: Bhattacharya
authors.affiliation: Animal Genetics Division, Indian Veterinary Research Institute, Izatnagar, Bareilly - 243 122, Uttar Pradesh, India.
authors.forename: Kush
authors.initials: K
authors.lastname: Shrivastava
authors.affiliation: Animal Genetics Division, Indian Veterinary Research Institute, Izatnagar, Bareilly - 243 122, Uttar Pradesh, India.
authors.forename: Bharat
authors.initials: B
authors.lastname: Bhushan
authors.affiliation: Standardization Division, Indian Veterinary Research Institute, Izatnagar, Bareilly - 243 122, Uttar Pradesh, India.
authors.forename: Ashok Kumar
authors.initials: AK
authors.lastname: Tiwari
authors.affiliation: Directorate of Poultry Research, Rajendranagar, Hyderabad, Telangana, India.
authors.forename: Vishesh
authors.initials: V
authors.lastname: Saxena
authors.affiliation: Animal Genetics Division, Indian Veterinary Research Institute, Izatnagar, Bareilly - 243 122, Uttar Pradesh, India.
authors.forename: Nihar Ranjan
authors.initials: NR
authors.lastname: Sahoo
authors.affiliation: Animal Genetics Division, Indian Veterinary Research Institute, Izatnagar, Bareilly - 243 122, Uttar Pradesh, India.
authors.forename: Deepak
authors.initials: D
authors.lastname: Sharma
keywords: Japanese quail; Mx1 gene; nucleotide sequencing; polymerase chain reaction-single-strand conformation polymorphism; polymorphism