title: Turning a Negative into a Positive: Conversion of a Homodimer into a Heterodimer Using Negative State Repertoires.
abstract: In this issue of Structure, Leaver-Fay et al. (2016) engineer bispecific antibodies using multistate computational protein design with negative state repertoires. In combination with additional mutations selected rationally, they produced antibodies that assembled as heterodimers with up to 93% purity.
authors.affiliation: Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, ON K1N 6N5, Canada.
authors.forename: James A
authors.initials: JA
authors.lastname: Davey
authors.affiliation: Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, ON K1N 6N5, Canada. Electronic address: rchica@uottawa.ca.
authors.forename: Roberto A
authors.initials: RA
authors.lastname: Chica
mesh_terms: D000906:Antibodies; D019281:Dimerization; D008958:Models, Molecular; D011506:Proteins
chemical_list: D000906:Antibodies; D011506:Proteins