title: An experimental model of affinity filtration for the isolation of egg white Lysozyme using Cibacron Blue immobilized to yeast cells.
abstract: An experimental model of affinity filtration process was designed using a macroligand composed by Cibacron Blue F3GA immobilized to yeast cells. Its performance was evaluated, at bench scale, through the recovery of egg white Lysozyme. The selective and reversible binding between the Cibacron ligand molecule and the enzyme is described. The separation of Lysozyme from the protein mixture included the application of stages such as affinity adsorption, concentration, diafiltration and elution. A tangential microfiltration system with an inorganic membrane was designed. The main finding was the development of the diafiltration operation, key stage in the enzyme isolation. The macroligand particle kept its integrity along the whole process and the degree of purity of the isolated Lysozyme was significant.
authors.affiliation: Laboratorio de Biología Molecular y Biotecnología, Departamento de Bioquímica, Universidad Nacional de San Luis, Ejército de los Andes 950, D5700HHW, San Luis, Argentina. Electronic address: ferraris@unsl.edu.ar.
authors.forename: María del Pilar
authors.initials: Mdel P
authors.lastname: Ferraris
authors.affiliation: Laboratorio de Biología Molecular y Biotecnología, Departamento de Bioquímica, Universidad Nacional de San Luis, Ejército de los Andes 950, D5700HHW, San Luis, Argentina.
authors.forename: Ulises A
authors.initials: UA
authors.lastname: Gonzalez
authors.affiliation: Laboratorio de Biología Molecular y Biotecnología, Departamento de Bioquímica, Universidad Nacional de San Luis, Ejército de los Andes 950, D5700HHW, San Luis, Argentina.
authors.forename: Carlos F
authors.initials: CF
authors.lastname: Aguilar
authors.affiliation: Laboratorio de Biología Molecular y Biotecnología, Departamento de Bioquímica, Universidad Nacional de San Luis, Ejército de los Andes 950, D5700HHW, San Luis, Argentina.
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Rodríguez
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D005374:Filtration / Q000379:methods*; D009113:Muramidase / Q000302:isolation & purification* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D014227:Triazines / Q000737:chemistry* / Q000378:metabolism
chemical_list: D014227:Triazines; C012842:Cibacron Blue F 3GA; D009113:Muramidase
keywords: Affinity filtration; Cibacron Blue; Hen egg white; Lysozyme isolation; Macroligand