title: Biocompatible hybrid silica nanobiocomposites for the efficient delivery of anti-staphylococcal drugs.
abstract: This work reports the non-surfactant templated synthesis and characterization of a new tyrosine-silica/antibiotics (TyR-SiO2/ATBs) nanocomposite, as well as both in vitro and in vivo cytotoxicity and antimicrobial activity against the microbial pathogen Staphylococcus aureus. The in vitro microbiological tests proved that the obtained nanobiostructure significantly enhance the antimicrobial activity of three commonly used antibiotics against S. aureus (i.e. erythromycin (ERI), gentamicin (GEN), and cloxacillin (CLO)) as revealed by the increased diameters of the growth inhibition zones and the decreased minimal inhibitory concentration values, as well as by the inhibitory effect of sub-lethal antibiotic concentrations on the ability of the respective pathogenic strains to adhere and colonize different substrata. These results, correlated with the lack of toxicity against mesenchymal stem cells along with an appropriate in vivo biodistribution highlight the promising therapeutic potential of this carrier that allows a decrease of the required active doses while significantly lessening the harmful side effects of the medication on the host organism.
authors.affiliation: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street No. 1-7, 011061 Bucharest, Romania.
authors.forename: Paul Cătălin
authors.initials: PC
authors.lastname: Balaure
authors.affiliation: "C. Nenitzescu" Organic Chemistry Department, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street No. 1-7, 011061 Bucharest, Romania.
authors.forename: Raisa Anamaria
authors.initials: RA
authors.lastname: Popa
authors.affiliation: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street No. 1-7, 011061 Bucharest, Romania. Electronic address: grumezescu@yahoo.com.
authors.forename: Alexandru Mihai
authors.initials: AM
authors.lastname: Grumezescu
authors.affiliation: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street No. 1-7, 011061 Bucharest, Romania.
authors.forename: Georgeta
authors.initials: G
authors.lastname: Voicu
authors.affiliation: Department of Inorganic Chemistry, Physical Chemistry & Electrochemistry, Faculty of Applied Chemistry and Materials Science, University POLITEHNICA of Bucharest, Bucharest, Romania.
authors.forename: Marius
authors.initials: M
authors.lastname: Rădulescu
authors.affiliation: Research Center for Microscopic Morphology and Immunology, University of Medicine and Pharmacy of Craiova, 2 Petru Rareş Street, 200349 Craiova, Romania.
authors.forename: Laurențiu
authors.initials: L
authors.lastname: Mogoantă
authors.affiliation: Research Center for Clinical and Experimental Medicine, University of Medicine and Pharmacy of Craiova, 2 Petru Rareş Street, 200349 Craiova, Romania.
authors.forename: Tudor-Adrian
authors.initials: TA
authors.lastname: Bălşeanu
authors.affiliation: Department of Pharmacognosy & Phytotherapy, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, 2 Petru Rareş Street, 200349 Craiova, Romania.
authors.forename: George Dan
authors.initials: GD
authors.lastname: Mogoşanu
authors.affiliation: University of Bucharest, Faculty of Biology, Microbiology Immunology Department, Aleea Portocalelor no 1-3, 060101 Bucharest, Romania; Research Institute of the Unversity of Bucharest, Spl. Independenței 91-95, Bucharest, Romania.
authors.forename: Mariana Carmen
authors.initials: MC
authors.lastname: Chifiriuc
authors.affiliation: "Ştefan S. Nicolau" Institute of Virology, 285 Mihai Bravu, 030304 Bucharest, Romania.
authors.forename: Coralia
authors.initials: C
authors.lastname: Bleotu
authors.affiliation: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street No. 1-7, 011061 Bucharest, Romania; University of Bucharest, Faculty of Biology, Microbiology Immunology Department, Aleea Portocalelor no 1-3, 060101 Bucharest, Romania; Research Institute of the Unversity of Bucharest, Spl. Independenței 91-95, Bucharest, Romania.
authors.forename: Alina Maria
authors.initials: AM
authors.lastname: Holban
authors.affiliation: Emergency University Hospital, Bucharest, Romania, "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Bolocan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry* / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry*; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008826:Microbial Sensitivity Tests / Q000379:methods; D012822:Silicon Dioxide / Q000737:chemistry*; D013203:Staphylococcal Infections / Q000188:drug therapy*; D013211:Staphylococcus aureus / Q000187:drug effects*; D014018:Tissue Distribution
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D012822:Silicon Dioxide
keywords: Drug delivery; Improved efficiency of antibiotics; Non-surfactant templated synthesis; Sol-gel polymerization; Staphylococcus aureus; Tyrosine-silica nanobiocomposites