title: SATB1 promotes prostate cancer metastasis by the regulation of epithelial-mesenchymal transition.
abstract: Special AT-rich sequence binding protein 1 (SATB1) plays important role in the regulation of chromatin structure and gene expression. Recent studies have indicated oncogenic role of SATB1. However, the function of SATB1 in prostate cancer progression and metastasis remains unclear. In this study SATB1 expression vector or siRNA was employed to modulate the expression level of SATB1 in prostate cancer cells and xenograft tumor in nude mouse model. Immunohistochemical analysis was performed on clinical prostate cancer samples. Silencing SATB1 inhibited the growth of DU-145 cells subcutaneous tumor in nude mice, while SATB1 overexpression promoted the growth of LNCaP cells subcutaneous tumor in nude mice. Immunohistochemical and Western blot analysis of the xenografts showed that silencing SATB1 led to decreased expression of vimentin and MMP2 and increased expression of E-cadherin, while SATB1 overexpression led to increased expression of vimentin and MMP2 and decreased expression of E-cadherin. Furthermore, SATB1, vimentin and MMP2 expression was increased significantly while E-cadherin expression was reduced significantly in clinical samples of prostate carcinoma with metastasis compared to prostate carcinoma without metastasis and benign prostate hyperplasia. Taken together, these findings suggest that the modulation of epithelial-mesenchymal transition by SATB1 may contribute to prostate cancer metastasis.
authors.affiliation: Department of Urinary Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China; Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, Xuzhou 221002, China.
authors.forename: Li-jun
authors.initials: LJ
authors.lastname: Mao
authors.affiliation: Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, Xuzhou 221002, China.
authors.forename: Chun-hua
authors.initials: CH
authors.lastname: Yang
authors.affiliation: Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, Xuzhou 221002, China.
authors.forename: Li
authors.initials: L
authors.lastname: Fan
authors.affiliation: Department of Urinary Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
authors.affiliation: Department of Urinary Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
authors.forename: Dong-rong
authors.initials: DR
authors.lastname: Yang
authors.affiliation: Department of Urinary Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
authors.forename: Bo-xin
authors.initials: BX
authors.lastname: Xue
authors.affiliation: Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, Xuzhou 221002, China. Electronic address: zhengjunnian@163.com.
authors.forename: Jun-nian
authors.initials: JN
authors.lastname: Zheng
authors.affiliation: Department of Urinary Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China. Electronic address: mljmlj05@126.com.
authors.forename: Yu-Xi
authors.initials: YX
authors.lastname: Shan
mesh_terms: D000818:Animals; D015703:Antigens, CD; D014408:Biomarkers, Tumor / Q000378:metabolism; D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D058750:Epithelial-Mesenchymal Transition; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D008297:Male; D036961:Matrix Attachment Region Binding Proteins / Q000235:genetics / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D040521:Subcutaneous Tissue / Q000473:pathology; D014746:Vimentin / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
chemical_list: D015703:Antigens, CD; D014408:Biomarkers, Tumor; C513384:CDH1 protein, human; D015820:Cadherins; D036961:Matrix Attachment Region Binding Proteins; C076189:SATB1 protein, human; D014746:Vimentin; D020778:Matrix Metalloproteinase 2
keywords: Invasion; Metastasis; Proliferation; Prostate cancer; SATB1