title: TWISTED DWARF1 Mediates the Action of Auxin Transport Inhibitors on Actin Cytoskeleton Dynamics.
abstract: Plant growth and architecture is regulated by the polar distribution of the hormone auxin. Polarity and flexibility of this process is provided by constant cycling of auxin transporter vesicles along actin filaments, coordinated by a positive auxin-actin feedback loop. Both polar auxin transport and vesicle cycling are inhibited by synthetic auxin transport inhibitors, such as 1-N-naphthylphthalamic acid (NPA), counteracting the effect of auxin; however, underlying targets and mechanisms are unclear. Using NMR, we map the NPA binding surface on the Arabidopsis thaliana ABCB chaperone TWISTED DWARF1 (TWD1). We identify ACTIN7 as a relevant, although likely indirect, TWD1 interactor, and show TWD1-dependent regulation of actin filament organization and dynamics and that TWD1 is required for NPA-mediated actin cytoskeleton remodeling. The TWD1-ACTIN7 axis controls plasma membrane presence of efflux transporters, and as a consequence act7 and twd1 share developmental and physiological phenotypes indicative of defects in auxin transport. These can be phenocopied by NPA treatment or by chemical actin (de)stabilization. We provide evidence that TWD1 determines downstream locations of auxin efflux transporters by adjusting actin filament debundling and dynamizing processes and mediating NPA action on the latter. This function appears to be evolutionary conserved since TWD1 expression in budding yeast alters actin polarization and cell polarity and provides NPA sensitivity.
authors.affiliation: Department of Biology, University of Fribourg, CH-1700 Fribourg, Switzerland.
authors.forename: Jinsheng
authors.identifier: 0000-0002-8131-1876
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Biology, University of Fribourg, CH-1700 Fribourg, Switzerland Department of Plant and Microbial Biology, University of Zurich, CH-8008 Zurich, Switzerland.
authors.forename: Aurelien
authors.initials: A
authors.lastname: Bailly
authors.affiliation: CEITEC-Central European Institute of Technology, Masaryk University, CZ-625 00 Brno, Czech Republic.
authors.forename: Marta
authors.initials: M
authors.lastname: Zwiewka
authors.affiliation: Department of Plant and Microbial Biology, University of Zurich, CH-8008 Zurich, Switzerland.
authors.forename: Valpuri
authors.initials: V
authors.lastname: Sovero
authors.affiliation: Department of Biology, University of Fribourg, CH-1700 Fribourg, Switzerland.
authors.forename: Martin
authors.identifier: 0000-0002-5642-2523
authors.initials: M
authors.lastname: Di Donato
authors.affiliation: Department of Biology, University of Fribourg, CH-1700 Fribourg, Switzerland.
authors.forename: Pei
authors.initials: P
authors.lastname: Ge
authors.affiliation: Department of Biology, University of Fribourg, CH-1700 Fribourg, Switzerland Institute of Evolutionary Biology and Environmental Studies, University of Zurich, CH-8057 Zurich, Switzerland.
authors.forename: Jacqueline
authors.identifier: 0000-0002-2981-9402
authors.initials: J
authors.lastname: Oehri
authors.affiliation: Department of Biology, University of Fribourg, CH-1700 Fribourg, Switzerland.
authors.forename: Bibek
authors.initials: B
authors.lastname: Aryal
authors.affiliation: Department of Biology, University of Fribourg, CH-1700 Fribourg, Switzerland.
authors.forename: Pengchao
authors.initials: P
authors.lastname: Hao
authors.affiliation: Max Planck Research Unit for Enzymology of Protein Folding, D-06099 Halle (Saale), Germany.
authors.forename: Miriam
authors.initials: M
authors.lastname: Linnert
authors.affiliation: Max Planck Research Unit for Enzymology of Protein Folding, D-06099 Halle (Saale), Germany Institute of Biochemistry and Biophysics (IQUIFIB), School of Pharmacy and Biochemistry, University of Buenos Aires, C1113AAD Buenos Aires, Argentina.
authors.forename: Noelia Inés
authors.initials: NI
authors.lastname: Burgardt
authors.affiliation: Max Planck Research Unit for Enzymology of Protein Folding, D-06099 Halle (Saale), Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Lücke
authors.affiliation: Max Planck Research Unit for Enzymology of Protein Folding, D-06099 Halle (Saale), Germany Department of Enzymology, Martin-Luther-University Halle-Wittenberg, Institute of Biochemistry and Biotechnology, D-06099 Halle, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Weiwad
authors.affiliation: Institute of Complex Systems, ICS-6: Structural Biochemistry, D-52425 Jülich, Germany.
authors.forename: Max
authors.initials: M
authors.lastname: Michel
authors.affiliation: Institute of Complex Systems, ICS-6: Structural Biochemistry, D-52425 Jülich, Germany.
authors.forename: Oliver H
authors.initials: OH
authors.lastname: Weiergräber
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, 28223 Pozuelo de Alarcón, Madrid, Spain.
authors.forename: Stephan
authors.initials: S
authors.lastname: Pollmann
authors.affiliation: LINV-DIPSAA, Università di Firenze, 50019 Florence, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Azzarello
authors.affiliation: LINV-DIPSAA, Università di Firenze, 50019 Florence, Italy.
authors.forename: Stefano
authors.identifier: 0000-0003-1752-3986
authors.initials: S
authors.lastname: Mancuso
authors.affiliation: University of Bonn, Mulliken Center for Theoretical Chemistry, Institute for Physical and Theoretical Chemistry, D-53115 Bonn, Germany.
authors.forename: Noel
authors.identifier: 0000-0003-3121-2084
authors.initials: N
authors.lastname: Ferro
authors.affiliation: Plant Global Educational Project, Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma 630-0192, Japan.
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Fukao
authors.affiliation: Cytoskeleton and Cancer Progression, Laboratory of Experimental Cancer Research, Department of Oncology, Luxembourg Institute of Health, L-1526 Luxembourg, Luxembourg.
authors.forename: Céline
authors.initials: C
authors.lastname: Hoffmann
authors.affiliation: Institute of Cell Dynamics and Imaging, University of Münster, D-48149 Münster, Germany.
authors.forename: Roland
authors.initials: R
authors.lastname: Wedlich-Söldner
authors.affiliation: Institute of Science and Technology Austria, A-3400 Klosterneuburg, Austria.
authors.forename: Jiří
authors.identifier: 0000-0002-8302-7596
authors.initials: J
authors.lastname: Friml
authors.affiliation: Cytoskeleton and Cancer Progression, Laboratory of Experimental Cancer Research, Department of Oncology, Luxembourg Institute of Health, L-1526 Luxembourg, Luxembourg.
authors.forename: Clément
authors.identifier: 0000-0001-6720-5615
authors.initials: C
authors.lastname: Thomas
authors.affiliation: Department of Biology, University of Fribourg, CH-1700 Fribourg, Switzerland Department of Plant and Microbial Biology, University of Zurich, CH-8008 Zurich, Switzerland markus.geisler@unifr.ch.
authors.forename: Markus
authors.identifier: 0000-0002-6641-5810
authors.initials: M
authors.lastname: Geisler
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport / Q000235:genetics / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D007210:Indoleacetic Acids / Q000378:metabolism*; D022021:Tacrolimus Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; C471286:TWD1 protein, Arabidopsis; D022021:Tacrolimus Binding Proteins