title: Hydropericardium Hepatitis Syndrome Emerged in Cherry Valley Ducks in China.
abstract: Since June 2015, a highly pathogenic disease occurred in duck flocks in China, causing pericardial effusion, enlarged discoloured liver, renal enlargement and haemorrhagic lung with a mortality ranging from 5% to 20%. Previous study confirmed that Fowl adenovirus group C (FAdV-C) and some field FAdVs isolates had been identified as causative agents of hydropericardium hepatitis syndrome (HHS) in chickens and geese world widely. In this study, we firstly report the isolation of FAdV-C from ducks with HHS. The two isolates, designated as SDSX and SDJX, were separated from liver samples using 9-day-old SPF chicken embryos and could cause severe cytopathic effects in duck and chicken embryonic kidney cells. The entire ORF sequences of hexon gene of the two isolates were amplified, sequenced and analysed by restriction fragment length polymorphism. Phylogenetic analysis of loop 1 sequences of hexon gene of FAdVs revealed that the two isolates were closely related to FAdV-C isolates, which could cause HHS in chickens. Experimental infection indicated that the isolate was high pathogenicity to 20-day-old ducks. Our study shows that the recently emerged HHS in ducks was caused by FAdV-C and may possess a potential risk to other poultry flocks.
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, China.
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Dou
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, China.
authors.forename: X
authors.initials: X
authors.lastname: Zheng
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Tang
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, China.
authors.forename: M
authors.initials: M
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Diao
mesh_terms: D000257:Adenoviridae Infections / Q000503:physiopathology / Q000662:veterinary* / Q000821:virology; D000818:Animals; D000259:Aviadenovirus / Q000145:classification / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D036022:Capsid Proteins / Q000235:genetics; D002642:Chick Embryo; D002681:China; D004372:Ducks; D004625:Embryo, Nonmammalian; D006505:Hepatitis; D016366:Open Reading Frames / Q000235:genetics; D010490:Pericardial Effusion; D010802:Phylogeny; D011201:Poultry Diseases / Q000503:physiopathology* / Q000821:virology; D017422:Sequence Analysis, DNA / Q000662:veterinary; D014774:Virulence
chemical_list: D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus
keywords: cherry valley duck; fowl adenovirus C; hydropericardium hepatitis syndrome