title: Uremic Pruritus Is Not Associated with Endocannabinoid Receptor 1 Gene Polymorphisms.
abstract: Uremic pruritus (UP) is a frequent and bothersome symptom in hemodialysis patients. Its etiology is not fully understood and that is why there is no specific treatment. The endocannabinoid system plays a role in many pathological conditions. There is reliable evidence on the association between cannabinoid system and pruritus. In our study, we aimed to evaluate whether genetic variations in the endocannabinoid receptor 1 (CNR1) gene can affect UP. The rs12720071, rs806368, rs1049353, rs806381, rs10485170, rs6454674, and rs2023239 polymorphisms of the CNR1 gene were genotyped in 159 hemodialysis patients and 150 healthy controls using two multiplex polymerase chain reactions and the minisequencing technique. No statistically significant relationship was found in any of the evaluated genotypes between patients with and without UP, even after excluding patients with diabetes and dyslipidemia. There were no differences between patients with UP and the control group. However, in the group of all HD patients, a significantly higher incidence of GA genotype and lower incidence in GG genotype in the polymorphism rs806381s were revealed versus the control group (p = 0.04). It seems that polymorphisms of the CNR1 gene are not associated with uremic pruritus.
authors.affiliation: Department of Dermatology, Venereology and Allergology, Wroclaw Medical University, 1 Chalubinskiego Street, 50-368 Wroclaw, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Heisig
authors.affiliation: Department of Endocrinology and Diabetology, Wroclaw Medical University, 4 Pasteura Street, 50-367 Wroclaw, Poland.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Łaczmański
authors.affiliation: Department of Dermatology, Venereology and Allergology, Wroclaw Medical University, 1 Chalubinskiego Street, 50-368 Wroclaw, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Reich
authors.affiliation: Department of Health Promotion, University School of Physical Education, 35 Paderewskiego Street, 51-612 Wroclaw, Poland.
authors.forename: Felicja
authors.initials: F
authors.lastname: Lwow
authors.affiliation: Department of Dermatology, Venereology and Allergology, Wroclaw Medical University, 1 Chalubinskiego Street, 50-368 Wroclaw, Poland.
authors.forename: Jacek C
authors.identifier: 0000-0003-0766-6342
authors.initials: JC
authors.lastname: Szepietowski
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011537:Pruritus / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D043884:Receptor, Cannabinoid, CB1 / Q000235:genetics*; D006435:Renal Dialysis / Q000379:methods
chemical_list: C571130:CNR1 protein, human; D043884:Receptor, Cannabinoid, CB1