title: New Cyclic Lipopeptides of the Iturin Class Produced by Saltern-Derived Bacillus sp. KCB14S006.
abstract: Salterns, one of the most extreme natural hypersaline environments, are a rich source of halophilic and halotolerant microorganisms, but they remain largely underexplored ecological niches in the discovery of bioactive secondary metabolites. In continued efforts to investigate the metabolic potential of microbial populations from chemically underexplored sites, three new lipopeptides named iturin F₁, iturin F₂ and iturin A₉ (1-3), along with iturin A₈ (4), were isolated from Bacillus sp. KCB14S006 derived from a saltern. The structures of the isolated compounds were established by 1D-, 2D-NMR and HR-ESIMS, and their absolute configurations were determined by applying advanced Marfey's method and CD spectroscopy. All isolates exhibited significant antifungal activities against various pathogenic fungi and moderate cytotoxic activities toward HeLa and src(ts)-NRK cell lines. Moreover, in an in vitro enzymatic assay, compound 4 showed a significant inhibitory activity against indoleamine 2,3-dioxygenase.
authors.affiliation: Anticancer Agent Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 363-883, Korea. sonski@kribb.re.kr.|University of Science and Technology (UST), Daejeon 305-333, Korea. sonski@kribb.re.kr.
authors.forename: Sangkeun
authors.initials: S
authors.lastname: Son
authors.affiliation: Anticancer Agent Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 363-883, Korea. ksk1230@kribb.re.kr.|University of Science and Technology (UST), Daejeon 305-333, Korea. ksk1230@kribb.re.kr.
authors.forename: Sung-Kyun
authors.initials: SK
authors.lastname: Ko
authors.affiliation: Anticancer Agent Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 363-883, Korea. jangmina@kribb.re.kr.|University of Science and Technology (UST), Daejeon 305-333, Korea. jangmina@kribb.re.kr.
authors.forename: Mina
authors.initials: M
authors.lastname: Jang
authors.affiliation: Anticancer Agent Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 363-883, Korea. jwkim@kribb.re.kr.
authors.forename: Jong Won
authors.initials: JW
authors.lastname: Kim
authors.affiliation: Anticancer Agent Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 363-883, Korea. sssktu@kribb.re.kr.|University of Science and Technology (UST), Daejeon 305-333, Korea. sssktu@kribb.re.kr.
authors.forename: Gil Soo
authors.initials: GS
authors.lastname: Kim
authors.affiliation: Anticancer Agent Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 363-883, Korea. scarscar@kribb.re.kr.
authors.forename: Jae Kyoung
authors.initials: JK
authors.lastname: Lee
authors.affiliation: Anticancer Agent Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 363-883, Korea. eunsooj@kribb.re.kr.
authors.forename: Eun Soo
authors.initials: ES
authors.lastname: Jeon
authors.affiliation: Chemical Biology Research Group, RIKEN Center for Sustainable Research Science, Saitama 351-0198, Japan. futamuray@riken.jp.
authors.forename: Yushi
authors.initials: Y
authors.lastname: Futamura
authors.affiliation: Anticancer Agent Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 363-883, Korea. ijryoo@kribb.re.kr.
authors.forename: In-Ja
authors.initials: IJ
authors.lastname: Ryoo
authors.affiliation: University of Science and Technology (UST), Daejeon 305-333, Korea. jslee@kribb.re.kr.|Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Korea. jslee@kribb.re.kr.
authors.forename: Jung-Sook
authors.initials: JS
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Wonkwang University, Iksan 570-749, Korea. hoh@wonkwang.ac.kr.
authors.forename: Hyuncheol
authors.initials: H
authors.lastname: Oh
authors.affiliation: Anticancer Agent Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 363-883, Korea. hongsoo@kribb.re.kr.|University of Science and Technology (UST), Daejeon 305-333, Korea. hongsoo@kribb.re.kr.
authors.forename: Young-Soo
authors.initials: YS
authors.lastname: Hong
authors.affiliation: Anticancer Agent Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 363-883, Korea. bykim@kribb.re.kr.|University of Science and Technology (UST), Daejeon 305-333, Korea. bykim@kribb.re.kr.
authors.forename: Bo Yeon
authors.initials: BY
authors.lastname: Kim
authors.affiliation: Chemical Biology Research Group, RIKEN Center for Sustainable Research Science, Saitama 351-0198, Japan. shunjitaka@riken.jp.|RIKEN-KRIBB Joint Research Unit, RIKRN Global Research Cluster, Saitama 351-0198, Japan. shunjitaka@riken.jp.
authors.forename: Shunji
authors.initials: S
authors.lastname: Takahashi
authors.affiliation: Chemical Biology Research Group, RIKEN Center for Sustainable Research Science, Saitama 351-0198, Japan. hisyo@riken.jp.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Osada
authors.affiliation: Anticancer Agent Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 363-883, Korea. jangjh@kribb.re.kr.|University of Science and Technology (UST), Daejeon 305-333, Korea. jangjh@kribb.re.kr.
authors.forename: Jae-Hyuk
authors.initials: JH
authors.lastname: Jang
authors.affiliation: Anticancer Agent Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 363-883, Korea. jsahn@kribb.re.kr.|University of Science and Technology (UST), Daejeon 305-333, Korea. jsahn@kribb.re.kr.
authors.forename: Jong Seog
authors.initials: JS
authors.lastname: Ahn
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology; D001407:Bacillus / Q000737:chemistry* / Q000378:metabolism*; D001419:Bacteria / Q000187:drug effects; D045744:Cell Line, Tumor; D005658:Fungi / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D055666:Lipopeptides / Q000737:chemistry* / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D055666:Lipopeptides; D010456:Peptides, Cyclic
keywords: antifungal; cytotoxic; indoleamine-2,3-dioxygenase; iturins; saltern-derived bacteria