title: Reduction potential and heme-pocket polarity in low potential cytochrome b5 of Giardia intestinalis.
abstract: Although it lacks mitochondria and the ability to synthesize heme, the protozoan parasite Giardia intestinalis encodes several heme proteins. This includes four members of the cytochrome b5 family, three of which are of similar size to mammalian cytochromes b5 but with reduction potentials that are 140 to 180mV lower. While no structures have yet been determined for any of these proteins, homology modeling points to an increase in heme pocket polarity as a reason for their low potentials. To test this we measured the reduction potentials of four mutants of Giardia cytochrome b5 isotype-I (gCYTB5-I) in which polar residues at two candidate positions (C84, Y51) in the heme pocket were changed to nonpolar ones (C84A, C84F; Y51L, Y51F). All mutants were expressed with comparable levels of heme incorporation and had UV-visible spectra consistent with low spin bis-histidyl coordination. These mutations increased the reduction potential by 18 to 57mV and highlight the influence of C84, which is a residue unique to gCYTB5-I and whose mutation to alanine caused the largest increase. The influence of these two residues plus that of Y61 reported previously accounts for much of the reduction potential difference between gCYTB5-I and microsomal cytochrome b5. A complementary triple mutant of the latter with the hydrophilic residues found in gCYTB5-I bound heme less effectively but nonetheless had a reduction potential that was 135mV lower than wild type.
authors.affiliation: Environmental and Life Sciences Graduate Program, Trent University, Canada.
authors.forename: Zhen Alice
authors.initials: ZA
authors.lastname: Yang
authors.affiliation: Biochemistry & Molecular Biology Program, Trent University, Canada.
authors.forename: Robert
authors.initials: R
authors.lastname: Pazdzior
authors.affiliation: Environmental and Life Sciences Graduate Program, Trent University, Canada; Biochemistry & Molecular Biology Program, Trent University, Canada.
authors.forename: Janet
authors.initials: J
authors.lastname: Yee
authors.affiliation: Environmental and Life Sciences Graduate Program, Trent University, Canada; Biochemistry & Molecular Biology Program, Trent University, Canada. Electronic address: srafferty@trentu.ca.
authors.forename: Steven
authors.initials: S
authors.lastname: Rafferty
mesh_terms: D000818:Animals; D002417:Cattle; D015786:Cytochromes b5 / Q000737:chemistry* / Q000378:metabolism*; D016829:Giardia lamblia / Q000378:metabolism*; D006418:Heme / Q000737:chemistry / Q000378:metabolism; D006420:Hemeproteins / Q000737:chemistry / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D016297:Mutagenesis, Site-Directed; D011485:Protein Binding; D017433:Protein Structure, Secondary; D015800:Protozoan Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D006420:Hemeproteins; D015800:Protozoan Proteins; D006418:Heme; D015786:Cytochromes b5
keywords: Cytochrome b5; Cytochromes; Giardia; Heme proteins