title: INHIBITION OF FATTY ACID DESATURASES IN Drosophila melanogaster LARVAE BLOCKS FEEDING AND DEVELOPMENTAL PROGRESSION.
abstract: Fatty acid desaturases are metabolic setscrews. To study their systemic impact on growth in Drosophila melanogaster, we inhibited fatty acid desaturases using the inhibitor CAY10566. As expected, the amount of desaturated lipids is reduced in larvae fed with CAY10566. These animals cease feeding soon after hatching, and their growth is strongly attenuated. A starvation program is not launched, but the expression of distinct metabolic genes is activated, possibly to mobilize storage material. Without attaining the normal size, inhibitor-fed larvae molt to the next stage indicating that the steroid hormone ecdysone triggers molting correctly. Nevertheless, after molting, expression of ecdysone-dependent regulators is not induced. While control larvae molt a second time, these larvae fail to do so and die after few days of straying. These effects are similar to those observed in experiments using larvae deficient for the fatty acid desaturase1 gene. Based on these data, we propose that the ratio of saturated to unsaturated fatty acids adjusts a sensor system that directs feeding behavior. We also hypothesize that loss of fatty acid desaturase activity leads to a block of the genetic program of development progression indirectly by switching on a metabolic compensation program.
authors.affiliation: Animal Genetics, Universität Tübingen, Tübingen, Germany.
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Animal Genetics, Universität Tübingen, Tübingen, Germany.
authors.forename: Tina Correia
authors.initials: TC
authors.lastname: da Cruz
authors.affiliation: Animal Genetics, Universität Tübingen, Tübingen, Germany.
authors.forename: Alicia
authors.initials: A
authors.lastname: Pulfemuller
authors.affiliation: Centre des Sciences du Goût et de l'Alimentation, Université de Bourgogne, Dijon, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Grégoire
authors.affiliation: Centre des Sciences du Goût et de l'Alimentation, Université de Bourgogne, Dijon, France.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Ferveur
authors.affiliation: iBV, Université Nice Sophia-Antipolis, Parc Valrose, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Moussian
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000201:enzymology / Q000254:growth & development / Q000502:physiology*; D004440:Ecdysone / Q000378:metabolism; D044943:Fatty Acid Desaturases / Q000235:genetics* / Q000378:metabolism; D005247:Feeding Behavior / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D007814:Larva / Q000201:enzymology / Q000254:growth & development / Q000502:physiology; D018978:Molting
chemical_list: D029721:Drosophila Proteins; D004440:Ecdysone; D044943:Fatty Acid Desaturases
keywords: Drosophila melanogaster; development; growth; insulin; lipids