title: Antibacterial and Anti-Inflammatory Activities of Physalis Alkekengi var. franchetii and Its Main Constituents.
abstract: This study was designed to determine whether the 50% EtOH fraction from AB-8 macroporous resin fractionation of a 70% EtOH extract of P. Alkekengi (50-EFP) has antibacterial and/or anti-inflammatory activity both in vivo and in vitro and to investigate the mechanism of 50-EFP anti-inflammatory activity. Additionally, this study sought to define the chemical composition of 50-EFP. Results indicated that 50-EFP showed significant antibacterial activity in vitro and efficacy in vivo. Moreover, 50-EFP significantly reduced nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor alpha (TNF-α), interleukin 1 (IL-1), and interleukin 6 (IL-6) production in lipopolysaccharide- (LPS-) stimulated THP-1 cells. Nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) (examined at the protein level) in THP-1 cells were suppressed by 50-EFP, which inhibited nuclear translocation of p65. Consistent with this anti-inflammatory activity in vitro, 50-EFP reduced inflammation in both animal models. Finally, seventeen compounds (8 physalins and 9 flavones) were isolated as major components of 50-EFP. Our data demonstrate that 50-EFP has antibacterial and anti-inflammatory activities both in vitro and in vivo. The anti-inflammatory effect appears to occur, at least in part, through the inhibition of nuclear translocation of p65. Moreover, physalins and flavones are probably the active components in 50-EFP that exert antibacterial and anti-inflammatory activities.
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, No. 28 Heping Road, Xiangfang District, Harbin, Heilongjiang 150040, China.
authors.forename: Zunpeng
authors.initials: Z
authors.lastname: Shu
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, No. 28 Heping Road, Xiangfang District, Harbin, Heilongjiang 150040, China.
authors.forename: Na
authors.initials: N
authors.lastname: Xing
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, No. 28 Heping Road, Xiangfang District, Harbin, Heilongjiang 150040, China.
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Departments of Biotechnology, Dalian Medical University, Dalian, Liaoning 116044, China.
authors.forename: Xinli
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, No. 28 Heping Road, Xiangfang District, Harbin, Heilongjiang 150040, China.
authors.forename: Bingqing
authors.initials: B
authors.lastname: Xu
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, No. 28 Heping Road, Xiangfang District, Harbin, Heilongjiang 150040, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, No. 28 Heping Road, Xiangfang District, Harbin, Heilongjiang 150040, China.
authors.forename: Haixue
authors.initials: H
authors.lastname: Kuang