title: MicroRNA-29b promotes the adipogenic differentiation of human adipose tissue-derived stromal cells.
abstract: Obesity is primarily characterized by the accumulation of large amounts of fat in adipose tissue. Within the adipose tissue, adipocytes are derived from adipose tissue-derived stromal cells (ADSCs) via a specialized cell lineage differentiation process, and ADSCs play a key role in the generation and metabolism of adipose tissue. This study investigated whether microRNAs (miRNAs) play a role in adipocyte differentiation.
  
  
  
  Using luciferase reporter and ChIP assays, the relationship between miR-29b, SP1, and TNF-α was examined.
  
  
  
  During the normal adipogenic differentiation of ADSCs, up-regulation of miR-29b promoted adipogenesis by enhancing SP1-mediated inhibition of TNF-α.
  
  
  
  This study investigated the regulatory role of miR-29b during the adipogenic differentiation of ADSCs and found that miR-29b is an effective positive regulator of adipogenesis.
authors.affiliation: Department of Histology and Embryology, Harbin Medical University, Harbin, People's Republic of China.
authors.forename: Xi-Mei
authors.initials: XM
authors.lastname: Zhang
authors.affiliation: Institute of Cancer Prevention and Treatment, Harbin Medical University, Harbin, People's Republic of China.
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Wang
authors.affiliation: Department of Respiratory, the Second Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China.
authors.forename: Dong-Ju
authors.initials: DJ
authors.lastname: Su
authors.affiliation: Department of Nephrology, the First Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Department of Histology and Embryology, Harbin Medical University, Harbin, People's Republic of China.
authors.forename: Qiu-Ming
authors.initials: QM
authors.lastname: Li
authors.affiliation: Teaching Experiment Center of Morphology, Harbin Medical University, Harbin, People's Republic of China.
authors.forename: Mei-Hua
authors.initials: MH
authors.lastname: Chi
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D050156:Adipogenesis / Q000502:physiology*; D000273:Adipose Tissue / Q000166:cytology*; D002454:Cell Differentiation / Q000502:physiology*; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism / Q000502:physiology*; D009765:Obesity / Q000378:metabolism; D016329:Sp1 Transcription Factor / Q000502:physiology; D017154:Stromal Cells / Q000166:cytology*; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology; D015854:Up-Regulation
chemical_list: C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D016329:Sp1 Transcription Factor; C108209:SP1 protein, human; D014409:Tumor Necrosis Factor-alpha