title: Overexpression of interleukin-33 is associated with poor prognosis of patients with glioma.
abstract: Interleukin-33 (IL-33) has shown diagnostic and prognostic values in multiple human cancers. However, there is little knowledge on the role of IL-33 in human gliomas and its association with disease prognosis. This study aimed to evaluate the value of IL-33 in the prognosis of glioma patients. The expression of IL-33 was determined and compared in surgical specimens from 86 glioma patients and 16 normal brain tissues. The associations of IL-33 expression with the clinicopathological features and prognosis of glioma patients were assessed. qRT-PCR assay showed higher IL-33 mRNA expression in glioma tissues than in normal brain tissue ( p < 0.001), and significantly higher IL-33 mRNA expression was detected in both low- and high-grade glioma tissues relative to normal brain tissues ( p < 0.001). Western blotting revealed elevated IL-33 protein levels in glioma tissues compared to those in normal brain tissues, and immunohistochemical staining showed higher IL-33 protein expression in glioma tissues than in normal brain tissues. IL-33 expression correlated with the glioma grade ( p < 0.001) and Karnofsky performance status score ( p = 0.024), and the glioma patients with high IL-33 expression had a shorter progression-free survival ( p < 0.001) and overall survival ( p < 0.001) than those with low IL-33 expression. The univariate and multivariate analyses showed that IL-33 overexpression and the glioma grade were independent factors of a poor prognosis in glioma patients. Therefore, IL-33 may be a promising biomarker for the detection of gliomas, and IL-33 expression is useful for predicting the prognosis of the disease.
authors.affiliation: a Department of Neurosurgery , Wuxi No. 2 People's Hospital Affiliated to Nanjing Medical University , Wuxi City , China.
authors.forename: Jianfei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: a Department of Neurosurgery , Wuxi No. 2 People's Hospital Affiliated to Nanjing Medical University , Wuxi City , China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: a Department of Neurosurgery , Wuxi No. 2 People's Hospital Affiliated to Nanjing Medical University , Wuxi City , China.
authors.forename: Weiyang
authors.initials: W
authors.lastname: Ji
authors.affiliation: a Department of Neurosurgery , Wuxi No. 2 People's Hospital Affiliated to Nanjing Medical University , Wuxi City , China.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ding
authors.affiliation: a Department of Neurosurgery , Wuxi No. 2 People's Hospital Affiliated to Nanjing Medical University , Wuxi City , China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Lu
mesh_terms: D000328:Adult; D000368:Aged; D001921:Brain / Q000378:metabolism*; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology* / Q000601:surgery; D018450:Disease Progression; D018572:Disease-Free Survival; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics / Q000473:pathology* / Q000601:surgery; D006801:Humans; D000067596:Interleukin-33 / Q000235:genetics / Q000378:metabolism*; D017567:Karnofsky Performance Status; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D055815:Young Adult
chemical_list: D000067596:Interleukin-33; D012333:RNA, Messenger
keywords: glioma; interleukin-33 (IL-33); prognosis