title: Proteomics analysis reveals distinct involvement of embryo and endosperm proteins during seed germination in dormant and non-dormant rice seeds.
abstract: Seed germination is a complex trait which is influenced by many genetic, endogenous and environmental factors, but the key event(s) associated with seed germination are still poorly understood. In present study, the non-dormant cultivated rice Yannong S and the dormant Dongxiang wild rice seeds were used as experimental materials, we comparatively investigated the water uptake, germination time course, and the differential proteome of the effect of embryo and endosperm on germination of these two types of seeds. A total of 231 and 180 protein spots in embryo and endosperm, respectively, showed a significant change in abundance during germination. We observed that the important proteins associated with seed germination included those involved in metabolism, energy production, protein synthesis and destination, storage protein, cell growth and division, signal transduction, cell defense and rescue. The contribution of embryo and endosperm to seed germination is different. In embryo, the proteins involved in amino acid activation, sucrose cleavage, glycolysis, fermentation and protein synthesis increased; in endosperm, the proteins involved in sucrose cleavage and glycolysis decreased, and those with ATP and CoQ synthesis and proteolysis increased. Our results provide some new knowledge to understand further the mechanism of seed germination.
authors.affiliation: Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Heng-Heng
authors.initials: HH
authors.lastname: Xu
authors.affiliation: Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Shu-Jun
authors.initials: SJ
authors.lastname: Liu
authors.affiliation: Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
authors.forename: Shun-Hua
authors.initials: SH
authors.lastname: Song
authors.affiliation: College of Life Science, Linyi University, Linyi 276005, China.
authors.forename: Rui-Xia
authors.initials: RX
authors.lastname: Wang
authors.affiliation: Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Wei-Qing
authors.initials: WQ
authors.lastname: Wang
authors.affiliation: Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China. Electronic address: sqsong@ibcas.ac.cn.
authors.forename: Song-Quan
authors.initials: SQ
authors.lastname: Song
mesh_terms: D056625:Endosperm / Q000502:physiology*; D018525:Germination / Q000502:physiology*; D012275:Oryza / Q000502:physiology*; D057445:Plant Dormancy; D010940:Plant Proteins / Q000378:metabolism; D020543:Proteome; D040901:Proteomics; D012639:Seeds / Q000502:physiology; D013395:Sucrose / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D020543:Proteome; D013395:Sucrose
keywords: Cultivated rice Yannong S; Dongxiang wild rice; Dormancy; Germination; Non-dormancy; Proteome analysis