title: Comprehensive RNA dataset of AGO2 associated RNAs in Jurkat cells following miR-21 over-expression.
abstract: We set out to identify miR-21 targets in Jurkat cells using a high-throughput biochemical approach (10.1016/j.biochi.2014.09.021[1]). Using a specific monoclonal antibody raised against AGO2, RISC complexes were immunopurified in Jurkat cells over-expressing miR-21 following lentiviral trasduction as well as in Jurkat control cells lines. A parallel immunoprecipitation using isotype-matched rat IgG was performed as a control. AGO2 associated mRNAs were profiled by microarray (GEO: GSE37212). AGO2 bound miRNAs were profiled by RNA-seq.
authors.affiliation: Dipartimento di Biotecnologie Cellulari ed Ematologia, "Sapienza" Università di Roma, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Carissimi
authors.affiliation: Dipartimento di Biotecnologie Cellulari ed Ematologia, "Sapienza" Università di Roma, Italy.
authors.forename: Teresa
authors.initials: T
authors.lastname: Colombo
authors.affiliation: Dipartimento di Biotecnologie Cellulari ed Ematologia, "Sapienza" Università di Roma, Italy.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Azzalin
authors.affiliation: Dipartimento di Biotecnologie Cellulari ed Ematologia, "Sapienza" Università di Roma, Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Cipolletta
authors.affiliation: Dipartimento di Biotecnologie Cellulari ed Ematologia, "Sapienza" Università di Roma, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Laudadio
authors.affiliation: Dipartimento di Biotecnologie Cellulari ed Ematologia, "Sapienza" Università di Roma, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Macino
authors.affiliation: Dipartimento di Biotecnologie Cellulari ed Ematologia, "Sapienza" Università di Roma, Italy.
authors.forename: Valerio
authors.initials: V
authors.lastname: Fulci
keywords: AGO2 immunoprecipitation; T-lymphocytes; miR-21; miRNA target