title: Histone Acetylation Induced Transformation of B-DNA to Z-DNA in Cells Probed through FT-IR Spectroscopy.
abstract: A nucleosome is made up of DNA and histones, and acetylation of histones perturbs the interaction of DNA and histones and thus affects the chromatin conformation and function. However, whether or how acetylation induces DNA conformation changes is still elusive. In this work, we applied FT-IR spectroscopy to monitor the DNA signals in cells as the histone acetylation was regulated by trichostatin A (TSA), a reversible inhibitor to histone deacetylases (HDACs). Our results unambiguously demonstrate the significant transformation of B-DNA to Z-DNA upon histone acetylation in the TSA treated HeLa cells. This is the first report providing the explicit experimental evidence for such a B-Z transformation of DNA in the epigenetic states of cells.
authors.affiliation: Institute of Technical Biology and Agriculture Engineering, Hefei Institutes of Physical Science, Key Laboratory of Ion-beam Bioengineering, Chinese Academy of Sciences , Hefei, China.|School of Nuclear Science and Technology, University of Science & Technology of China , Hefei, China.|Henan Key Laboratory of Ion-beam Bioengineering, School of Physical Engineering, Zhengzhou University , Zhengzhou, China.
authors.forename: Fengqiu
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Institute of Technical Biology and Agriculture Engineering, Hefei Institutes of Physical Science, Key Laboratory of Ion-beam Bioengineering, Chinese Academy of Sciences , Hefei, China.|School of Nuclear Science and Technology, University of Science & Technology of China , Hefei, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Institute of Technical Biology and Agriculture Engineering, Hefei Institutes of Physical Science, Key Laboratory of Ion-beam Bioengineering, Chinese Academy of Sciences , Hefei, China.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Yan
authors.affiliation: Institute of Technical Biology and Agriculture Engineering, Hefei Institutes of Physical Science, Key Laboratory of Ion-beam Bioengineering, Chinese Academy of Sciences , Hefei, China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000107:Acetylation; D059371:DNA, B-Form / Q000737:chemistry / Q000378:metabolism*; D043542:DNA, Z-Form / Q000737:chemistry / Q000378:metabolism*; D006367:HeLa Cells; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology; D009690:Nucleic Acid Conformation; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D059371:DNA, B-Form; D043542:DNA, Z-Form; D006657:Histones; D006877:Hydroxamic Acids; C012589:trichostatin A