title: Comparison of the response using ICR mice derived from three different sources to ethanol/hydrochloric acid-induced gastric injury.
abstract: Animal models for gastric ulcers produced by physical, pharmacological and surgical methods have been widely employed to evaluate therapeutic drugs and investigate the mechanism of action of this disease. ICR mice were selected to produce this model, even though several mice and rats have been widely used in studies of gastric ulcers. To compare the responses of ICR mice obtained from three different sources to gastric ulcer inducers, alterations in gastric injury, histopathological structure, and inflammation were measured in Korl:ICR (Korea NIFDS source), A:ICR (USA source) and B:ICR (Japan source) treated with three concentrations of ethanol (EtOH) (50, 70, and 90%) in 150 mM hydrochloric acid (HCl) solution. Firstly, the stomach lesion index gradually increased as the EtOH concentration increased in three ICR groups. Moreover, a significant increase in the level of mucosal injury, edema and the number of inflammatory cells was similarly detected in the EtOH/HCl treated group compared with the vehicle treated group in three ICR groups. Furthermore, the number of infiltrated mast cells and IL-1β expression were very similar in the ICR group derived from three different sources, although some differences in IL-1β expression were detected. Especially, the level of IL-1β mRNA in 50 and 90EtOH/HCl treated group was higher in Korl:ICR and A:ICR than B:ICR. Overall, the results of this study suggest that Korl:ICR, A:ICR and B:ICR derived from different sources have an overall similar response to gastric ulcer induced by EtOH/HCl administration, although there were some differences in the magnitude of their responses.
authors.affiliation: Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, Korea.
authors.forename: Sung Hwa
authors.initials: SH
authors.lastname: Song
authors.affiliation: Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, Korea.
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Kim
authors.affiliation: Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, Korea.
authors.forename: Jun
authors.initials: J
authors.lastname: Go
authors.affiliation: Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, Korea.
authors.forename: Eun Kyoung
authors.initials: EK
authors.lastname: Koh
authors.affiliation: Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, Korea.
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Sung
authors.affiliation: Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, Korea.
authors.forename: Hyun Ah
authors.initials: HA
authors.lastname: Lee
authors.affiliation: Laboratory Animal Resources Division, Toxicological Evaluation Research Department, National Institute of Food and Drug Safety Evaluation, Cheongju 28159, Korea.
authors.forename: Kyung Min
authors.initials: KM
authors.lastname: Choi
authors.affiliation: Laboratory Animal Resources Division, Toxicological Evaluation Research Department, National Institute of Food and Drug Safety Evaluation, Cheongju 28159, Korea.
authors.forename: Hae Deun
authors.initials: HD
authors.lastname: Kim
authors.affiliation: Department of Pharmacy, College of Pharmacy, Pusan National University, Busan 46241, Korea.
authors.forename: Young Suk
authors.initials: YS
authors.lastname: Jung
authors.affiliation: College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea.
authors.forename: Kil Soo
authors.initials: KS
authors.lastname: Kim
authors.affiliation: Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, Korea.
authors.forename: Dae Youn
authors.initials: DY
authors.lastname: Hwang
keywords: EtOH/HCl; Gastric ulcer; Hemorrhagic lesions; IL-1β; Korl:ICR mice; Mast cells