title: Differential Expression of Adenosine P1 Receptor ADORA1 and ADORA2A Associated with Glioma Development and Tumor-Associated Epilepsy.
abstract: Level of adenosine, an endogenous astrocyte-based neuromodulator, is primarily regulated by adenosine P1 receptors. This study assessed expression of adenosine P1 receptors, ADORA1 (adenosine A1 receptor) and ADORA2A (adenosine A2a receptor) and their association with glioma development and epilepsy in glioma patients. Expression of ADORA1/ADORA2A was assessed immunohistochemically in 65 surgically removed glioma tissue and 21 peri-tumor tissues and 8 cases of normal brain tissues obtained from hematoma patients with cerebral trauma. Immunofluorescence, Western blot, and qRT-PCR were also used to verify immunohistochemical data. Adenosine P1 receptor ADORA1 and ADORA2A proteins were localized in the cell membrane and cytoplasm and ADORA1/ADORA2A immunoreactivity was significantly stronger in glioma and peri-tumor tissues that contained infiltrating tumor cells than in normal brain tissues (p < 0.05). The World Health Organization (WHO) grade III gliomas expressed even higher level of ADORA1 and ADORA2A. Western blot and qRT-PCR confirmed immunohistochemical data. Moreover, higher levels of ADORA1 and ADORA2A expression occurred in high-grade gliomas, in which incidence of epilepsy were lower (p < 0.05). In contrast, a lower level of ADORA1/ADORA2A expression was found in peri-tumor tissues with tumor cell presence from patients with epilepsy compared to patients without epilepsy (p < 0.05). The data from the current study indicates that dysregulation in ADORA1/ADORA2A expression was associated with glioma development, whereas low level of ADORA1/ADORA2A expression could increase susceptibility of tumor-associated epilepsy.
authors.affiliation: Departments of Neurosurgery, Xiangya Hospital, The Central South University, Changsha, 410008, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Huang
authors.affiliation: Departments of Neurology, Xiangya Hospital, The Central South University, 87 Xiangya Road, Changsha, 410008, China.
authors.forename: Ming-Na
authors.initials: MN
authors.lastname: Chen
authors.affiliation: Departments of Neurology, Xiangya Hospital, The Central South University, 87 Xiangya Road, Changsha, 410008, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.affiliation: Departments of Neurology, Xiangya Hospital, The Central South University, 87 Xiangya Road, Changsha, 410008, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.affiliation: Departments of Neurology, Xiangya Hospital, The Central South University, 87 Xiangya Road, Changsha, 410008, China.
authors.forename: Yu-Jiao
authors.initials: YJ
authors.lastname: He
authors.affiliation: Departments of Neurology, Xiangya Hospital, The Central South University, 87 Xiangya Road, Changsha, 410008, China.
authors.forename: Guo-Liang
authors.initials: GL
authors.lastname: Li
authors.affiliation: Departments of Neurology, Xiangya Hospital, The Central South University, 87 Xiangya Road, Changsha, 410008, China.
authors.forename: Shu-Yu
authors.initials: SY
authors.lastname: Li
authors.affiliation: Departments of Neurology, Xiangya Hospital, The Central South University, 87 Xiangya Road, Changsha, 410008, China.
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Liu
authors.affiliation: Departments of Neurology, Xiangya Hospital, The Central South University, 87 Xiangya Road, Changsha, 410008, China. 815236629@qq.com.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Long
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002648:Child; D004827:Epilepsy / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D043682:Receptor, Adenosine A1 / Q000096:biosynthesis* / Q000235:genetics; D043705:Receptor, Adenosine A2A / Q000096:biosynthesis* / Q000235:genetics; D055815:Young Adult
chemical_list: D043682:Receptor, Adenosine A1; D043705:Receptor, Adenosine A2A
keywords: ADORA1; ADORA2A; Epilepsy; Glioma; Immunohistochemistry