title: Field-Friendly Test for Monitoring Multiple Immune Response Markers during Onset and Treatment of Exacerbated Immunity in Leprosy.
abstract: Acute inflammatory reactions represent the major cause of irreversible neuropathy in leprosy. These tissue-destroying episodes have considerable overlap with acute immunological complications (flares) in several chronic (autoimmune) diseases that similarly warrant early detection. However, the lack of diagnostic tests impedes early diagnosis of these reactions. Here, we evaluated a user-friendly multiplex lateral flow assay for the simultaneous detection of IP-10 and anti-phenolic glycolipid I antibodies for longitudinally monitoring early onset and treatment of leprosy reactions.
authors.affiliation: Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Paul L A M
authors.initials: PLAM
authors.lastname: Corstjens
authors.affiliation: Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Anouk
authors.initials: A
authors.lastname: van Hooij
authors.affiliation: Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Elisa M
authors.initials: EM
authors.lastname: Tjon Kon Fat
authors.affiliation: Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Susan J F
authors.initials: SJF
authors.lastname: van den Eeden
authors.affiliation: Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Louis
authors.initials: L
authors.lastname: Wilson
authors.affiliation: Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands a.geluk@lumc.nl.
authors.forename: Annemieke
authors.initials: A
authors.lastname: Geluk
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology*; D015415:Biomarkers / Q000097:blood; D054357:Chemokine CXCL10 / Q000097:blood* / Q000276:immunology; D042241:Early Diagnosis; D004797:Enzyme-Linked Immunosorbent Assay; D006017:Glycolipids / Q000276:immunology*; D006801:Humans; D007918:Leprosy / Q000150:complications / Q000276:immunology* / Q000628:therapy; D015166:Monitoring, Immunologic / Q000379:methods*; D009166:Mycobacterium leprae / Q000276:immunology; D019095:Point-of-Care Systems
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D015415:Biomarkers; C114753:CXCL10 protein, human; D054357:Chemokine CXCL10; D006017:Glycolipids; C045169:phenolic glycolipid I, Mycobacterium leprae