title: PolyA-Mediated DNA Assembly on Gold Nanoparticles for Thermodynamically Favorable and Rapid Hybridization Analysis.
abstract: Understanding the behavior of biomolecules on nanointerface is critical in bioanalysis, which is great challenge due to the instability and the difficulty to control the orientation and loading density of biomolecules. Here, we investigated the thermodynamics and kinetics of DNA hybridization on gold nanoparticle, with the aim to improve the efficiency and speed of DNA analysis. We achieved precise and quantitative surface control by applying a recently developed poly adenines (polyA)-based assembly strategy on gold nanoparticles (DNA-AuNPs). PolyA served as an effective anchoring block based on the preferential binding with the AuNP surface and the appended recognition block adopted an upright conformation that favors DNA hybridization. The lateral spacing and surface density of DNA on AuNPs can be systematically modulated by adjusting the length of polyA block. We found the stability of duplex on AuNP was enhanced with the increasing length of polyA block. When the length of polyA block reached to 40 bases, the thermodynamic properties were more similar to that of duplex in solution. Fast hybridization rate was observed on the diblock DNA-AuNPs and was increased along with the length of polyA block. We consider the high stability and excellent hybridization performance come from the minimization of the DNA-DNA and DNA-AuNP interactions with the use of polyA block. This study provides better understanding of the behavior of biomolecules on the nanointerface and opens new opportunities to construct high-efficiency and high-speed biosensors for DNA analysis.
authors.affiliation: Institute of Advanced Materials, Nanjing University of Posts and Telecommunications , Nanjing 210023, People's Republic of China.|Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Song
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University , Shanghai 200241, People's Republic of China.
authors.forename: Juwen
authors.initials: J
authors.lastname: Shen
authors.affiliation: Institute of Advanced Materials, Nanjing University of Posts and Telecommunications , Nanjing 210023, People's Republic of China.
authors.forename: Shao
authors.initials: S
authors.lastname: Su
authors.affiliation: Institute of Advanced Materials, Nanjing University of Posts and Telecommunications , Nanjing 210023, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chao
authors.affiliation: Chemistry Department, King Saud University , Riyadh 11451, Saudi Arabia.
authors.forename: Ali
authors.initials: A
authors.lastname: Aldalbahi
authors.affiliation: Johns Hopkins University, Baltimore, Maryland 21211, United States.
authors.forename: Ziang
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, People's Republic of China.
authors.forename: Shiping
authors.initials: S
authors.lastname: Song
authors.affiliation: Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, People's Republic of China.
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
authors.affiliation: Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, People's Republic of China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Zuo
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University , Shanghai 200241, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Institute of Advanced Materials, Nanjing University of Posts and Telecommunications , Nanjing 210023, People's Republic of China.
authors.forename: Lianhui
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University , Shanghai 200241, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Pei
mesh_terms: D004247:DNA / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D007700:Kinetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D011061:Poly A; D006046:Gold; D004247:DNA