title: Qualitative and Quantitative Assessments of Aconiti Lateralis Radix Praeparata Using High-Performance Liquid Chromatography Coupled with Diode Array Detection and Hybrid Ion Trap-Time-of-Flight Mass Spectrometry.
abstract: Dual roles have been widely disclosed for Aconiti Lateralis Radix Praeparata (Chinese name: Fuzi) by acting as an effective herbal medicine accompanied with high toxicity risk and narrow therapeutic range. Aconite alkaloids, also known as diterpenoid alkaloids (DAs), have been proved to be primary therapeutic as well as toxic material basis of Fuzi. In the present study, we aim to characterize qualitative profile along with quantitative features of DAs in Fuzi, which could benefit the quality control of Fuzi as well as its safe medication in clinic. Chromatographic fingerprinting was achieved using 12 batches of crude materials collected from different habitats (similarities >0.9) by hyphenating liquid chromatography (LC) with diode array detection. Simultaneous determination of six primary DAs, namely benzoylmesaconine, benzoylaconine, benzoylhypaconine, mesaconine, aconitine and hypaconitine, was also carried out. The developed method was systematically validated and successfully applied for quantitative characterization. Significant variations were observed regarding the contents of those six analytes among different raw materials. A total of 99 DAs were detected and characterized, including 9 unambiguous identities and 77 putative assignments, as well as 13 unknown compounds, from the representative extract by LC coupled with hybrid ion trap-time-of-flight mass spectrometry. The findings obtained herein could favor in-depth understanding of the chemical composition of DAs and the quality control of Fuzi.
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China Baotou Medical College, Baotou 014060, China.
authors.forename: Na
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Yuelin
authors.initials: Y
authors.lastname: Song
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Song
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Shepo
authors.initials: S
authors.lastname: Shi
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Yunfang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China drlj666@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Tu
mesh_terms: D000157:Aconitine / Q000032:analysis / Q000737:chemistry; D031841:Aconitum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry
chemical_list: D004365:Drugs, Chinese Herbal; D000157:Aconitine