title: miR-375 negatively regulates porcine preadipocyte differentiation by targeting BMPR2.
abstract: The differentiation of preadipocytes into adipose tissues is tightly regulated by various factors including miRNAs and cytokines. In this study, taking advantage of isolated porcine primary preadipocytes, we showed that ectopic expression of miR-375 could change preadipocyte differentiation. In addition, bone morphogenetic protein receptor 2 (BMPR2) was identified as a direct target of miR-375. Silencing BMPR2 had the same inhibition effects as overexpressing miR-375 on the preadipocyte differentiation. Together, we demonstrated that miR-375 is a negative regulator of adipogenic differentiation using porcine primary preadipocytes. These results clarified the role of miR-375 in ex vivo adipogenic differentiation.
authors.affiliation: College of Animal Sciences, Jilin University, Changchun, China.
authors.forename: Siyuan
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Animal Sciences, Jilin University, Changchun, China.
authors.forename: Guangjie
authors.initials: G
authors.lastname: Sun
authors.affiliation: College of Animal Sciences, Jilin University, Changchun, China.
authors.forename: Bao
authors.initials: B
authors.lastname: Yuan
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural Science and Technology College, China.
authors.forename: Lianjiang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Animal Sciences, Jilin University, Changchun, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: College of Animal Sciences, Jilin University, Changchun, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Jiang
authors.affiliation: College of Animal Sciences, Jilin University, Changchun, China.
authors.forename: Lisheng
authors.initials: L
authors.lastname: Dai
authors.affiliation: College of Animal Sciences, Jilin University, Changchun, China.
authors.forename: Jiabao
authors.initials: J
authors.lastname: Zhang
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D050156:Adipogenesis; D000818:Animals; D052006:Bone Morphogenetic Protein Receptors, Type II / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D035683:MicroRNAs / Q000378:metabolism*; D013552:Swine
chemical_list: D035683:MicroRNAs; D052006:Bone Morphogenetic Protein Receptors, Type II
keywords: BMPR2; adipogenic differentiation; ex vivo; miR-375; primary preadipocyte