title: Dietary phytic acid prevents fatty liver by reducing expression of hepatic lipogenic enzymes and modulates gut microflora in rats fed a high-sucrose diet.
abstract: OBJECTIVE
  The aim of this study was to investigate the effect of phytic acid (PA) on fatty liver and gut microflora in rats fed a high-sucrose (HSC) diet.
  
  
  METHODS
  Three groups of rats were fed a high-starch (HSR) diet or an HSC diet with or without 1.02% sodium PA for 12 d. We evaluated hepatic weight, total lipids, and triacylglycerol (TG) levels, the activities and expression of hepatic lipogenic enzymes (glucose-6-phosphate dehydrogenase, malic enzyme 1, and fatty acid synthetase), and fecal microflora.
  
  
  RESULTS
  The HSC diet significantly increased hepatic total lipids and TG levels, and the activities and expression of the hepatic lipogenic enzymes compared with the HSR diet. These upregulations were clearly suppressed by dietary PA. Consumption of PA elevated the fecal ratio of Lactobacillus spp. and depressed the ratio of Clostridium cocoides, and suppressed the elevation in the ratio of C. leptum induced by the HSC diet.
  
  
  CONCLUSIONS
  This work showed that dietary PA ameliorates sucrose-induced fatty liver through reducing the expression of hepatic lipogenesis genes and modulates gut microflora in rats.
authors.affiliation: Faculty of Human Life Sciences, Fuji Women's University, Ishikari, Japan.
authors.forename: Ayaka
authors.initials: A
authors.lastname: Sekita
authors.affiliation: Faculty of Human Life Sciences, Fuji Women's University, Ishikari, Japan. Electronic address: yokazaki@fujijoshi.ac.jp.
authors.forename: Yukako
authors.initials: Y
authors.lastname: Okazaki
authors.affiliation: Institution of Life Sciences and Nutrition, Kita-ku, Japan.
authors.forename: Tetsuyuki
authors.initials: T
authors.lastname: Katayama
mesh_terms: D000818:Animals; D004032:Diet / Q000379:methods*; D019422:Dietary Sucrose / Q000008:administration & dosage*; D004195:Disease Models, Animal; D005234:Fatty Liver / Q000201:enzymology / Q000517:prevention & control*; D000069196:Gastrointestinal Microbiome / Q000187:drug effects*; D050155:Lipogenesis / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000382:microbiology; D008297:Male; D010833:Phytic Acid / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019422:Dietary Sucrose; D010833:Phytic Acid
keywords: Fatty acid synthetase; Fatty liver; Glucose-6-phosphate dehydrogenase; Gut microflora; High-sucrose diet; Malic enzyme 1; Phytic acid