title: IDENTIFICATION OF GENES PREFERENTIALLY EXPRESSED DURING ASEXUAL SPORULATION IN PORPHYRA YEZOENSIS GAMETOPHYTES (BANGIALES, RHODOPHYTA)(1).
abstract: Asexual reproduction via archeospores in Porphyra yezoensis Ueda gametophytes is a very valuable character to nori farming; however, there is little information available on the molecular basis of the developmental process. To identify genes involved in the Porphyra asexual sporulation, we compared the gene expression profiles derived from four developmental stages of the life cycle (three from gametophytes; one from sporophytes) using cDNA macroarray, which includes 4,896 nonredundant expressed sequence tag (EST) groups. Candidate genes were screened by two different macroarray data analyses combined with reverse transcription-PCR (RT-PCR) analysis or Northern analysis. RT-PCR analysis revealed that nine genes (one: similarity to 5'-3' exoribonuclease; the other eight: no sequence similarity to known proteins) were expressed with a gametophyte (G)-specific manner, and two genes (named ASPO2608, ASPO1527) were expressed only in gametophytes that formed archeospores. The deduced amino acid sequences for the latter two genes are predicted to contain signal peptides for secretion at their N-termini. Northern analysis revealed that expression levels of Calvin cycle genes in the gametophytic stage that formed archeospores (G-A stage) were higher than those of the gametophyte blade with no archeospores (G-NA stage). In the macroarray analysis based on the rank data of G-preferentially expressed genes, which were detected in the previous P. yezoensis EST analysis, one gene encoding the cyclase associated protein (CAP) exhibited a change upwardly in the G-A stage >1,000 ranks to the G-NA stage. We propose that ASPO2608 and CAP may function in a signaling pathway of asexual sporulation.
authors.affiliation: Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanKazusa DNA Research Institute, Kisarazu, Chiba 292-0812, JapanGraduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanKazusa DNA Research Institute, Kisarazu, Chiba 292-0812, JapanGraduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanFaculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanKazusa DNA Research Institute, Kisarazu, Chiba 292-0812, JapanFaculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Kitade
authors.affiliation: Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanKazusa DNA Research Institute, Kisarazu, Chiba 292-0812, JapanGraduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanKazusa DNA Research Institute, Kisarazu, Chiba 292-0812, JapanGraduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanFaculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanKazusa DNA Research Institute, Kisarazu, Chiba 292-0812, JapanFaculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan.
authors.forename: Erika
authors.initials: E
authors.lastname: Asamizu
authors.affiliation: Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanKazusa DNA Research Institute, Kisarazu, Chiba 292-0812, JapanGraduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanKazusa DNA Research Institute, Kisarazu, Chiba 292-0812, JapanGraduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanFaculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanKazusa DNA Research Institute, Kisarazu, Chiba 292-0812, JapanFaculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Fukuda
authors.affiliation: Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanKazusa DNA Research Institute, Kisarazu, Chiba 292-0812, JapanGraduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanKazusa DNA Research Institute, Kisarazu, Chiba 292-0812, JapanGraduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanFaculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanKazusa DNA Research Institute, Kisarazu, Chiba 292-0812, JapanFaculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan.
authors.forename: Maiko
authors.initials: M
authors.lastname: Nakajima
authors.affiliation: Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanKazusa DNA Research Institute, Kisarazu, Chiba 292-0812, JapanGraduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanKazusa DNA Research Institute, Kisarazu, Chiba 292-0812, JapanGraduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanFaculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanKazusa DNA Research Institute, Kisarazu, Chiba 292-0812, JapanFaculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan.
authors.forename: Shuuji
authors.initials: S
authors.lastname: Ootsuka
authors.affiliation: Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanKazusa DNA Research Institute, Kisarazu, Chiba 292-0812, JapanGraduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanKazusa DNA Research Institute, Kisarazu, Chiba 292-0812, JapanGraduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanFaculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanKazusa DNA Research Institute, Kisarazu, Chiba 292-0812, JapanFaculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan.
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Endo
authors.affiliation: Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanKazusa DNA Research Institute, Kisarazu, Chiba 292-0812, JapanGraduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanKazusa DNA Research Institute, Kisarazu, Chiba 292-0812, JapanGraduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanFaculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanKazusa DNA Research Institute, Kisarazu, Chiba 292-0812, JapanFaculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tabata
authors.affiliation: Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanKazusa DNA Research Institute, Kisarazu, Chiba 292-0812, JapanGraduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanKazusa DNA Research Institute, Kisarazu, Chiba 292-0812, JapanGraduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanFaculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, JapanKazusa DNA Research Institute, Kisarazu, Chiba 292-0812, JapanFaculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan.
authors.forename: Naotsune
authors.initials: N
authors.lastname: Saga
keywords: Rhodophyta; archeospore; asexual sporulation; cDNA macroarray; gametophyte