title: Homo- and heteromeric interaction strengths of the synergistic antimicrobial peptides PGLa and magainin 2 in membranes.
abstract: PGLa and magainin 2 (MAG2) are amphiphilic α-helical frog peptides with synergistic antimicrobial activity. In vesicle leakage assays we observed the strongest synergy for equimolar mixtures of PGLa and MAG2. This result was consistent with solid-state (15)N-NMR data on the helix alignment in model membranes. The Hill coefficients determined from the vesicle leakage data showed that the heterodimeric (PGLa-MAG2) interactions were stronger than the homodimeric (PGLa-PGLa and MAG2-MAG2) interactions. This result was also reflected in the free energy of dimerization determined from oriented circular dichroism and quantitative solid-state (19)F-NMR analysis.
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute of Organic Chemistry, Fritz-Haber-Weg 6, 76131, Karlsruhe, Germany.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Zerweck
authors.affiliation: KIT, Institute of Biological Interfaces (IBG-2), POB 3640, 76021, Karlsruhe, Germany.
authors.forename: Erik
authors.initials: E
authors.lastname: Strandberg
authors.affiliation: KIT, Institute of Biological Interfaces (IBG-2), POB 3640, 76021, Karlsruhe, Germany.
authors.forename: Jochen
authors.initials: J
authors.lastname: Bürck
authors.affiliation: KIT, Institute of Biological Interfaces (IBG-2), POB 3640, 76021, Karlsruhe, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Reichert
authors.affiliation: KIT, Institute of Biological Interfaces (IBG-2), POB 3640, 76021, Karlsruhe, Germany.
authors.forename: Parvesh
authors.initials: P
authors.lastname: Wadhwani
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute of Organic Chemistry, Fritz-Haber-Weg 6, 76131, Karlsruhe, Germany.
authors.forename: Olga
authors.initials: O
authors.lastname: Kukharenko
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute of Organic Chemistry, Fritz-Haber-Weg 6, 76131, Karlsruhe, Germany. anne.ulrich@kit.edu.|KIT, Institute of Biological Interfaces (IBG-2), POB 3640, 76021, Karlsruhe, Germany. anne.ulrich@kit.edu.
authors.forename: Anne S
authors.initials: AS
authors.lastname: Ulrich
mesh_terms: D000595:Amino Acid Sequence; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism*; D004357:Drug Synergism; D054806:Magainins / Q000737:chemistry / Q000494:pharmacology*; D013816:Thermodynamics
chemical_list: D023181:Antimicrobial Cationic Peptides; D054806:Magainins; C045866:peptide-Gly-Leu-amide
keywords: Hill coefficients; Membrane-active amphiphilic helices; Peptide dimerization; Solid-state NMR; Synergistic antimicrobial peptides; Vesicle leakage assay