title: Host-parasite oscillation dynamics and evolution in a compartmentalized RNA replication system.
abstract: To date, various cellular functions have been reconstituted in vitro such as self-replication systems using DNA, RNA, and proteins. The next important challenges include the reconstitution of the interactive networks of self-replicating species and investigating how such interactions generate complex ecological behaviors observed in nature. Here, we synthesized a simple replication system composed of two self-replicating host and parasitic RNA species. We found that the parasitic RNA eradicates the host RNA under bulk conditions; however, when the system is compartmentalized, a continuous oscillation pattern in the population dynamics of the two RNAs emerges. The oscillation pattern changed as replication proceeded mainly owing to the evolution of the host RNA. These results demonstrate that a cell-like compartment plays an important role in host-parasite ecological dynamics and suggest that the origin of the host-parasite coevolution might date back to the very early stages of the evolution of life.
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan; Japan Society for the Promotion of Science, Chiyoda-ku, Tokyo 102-0083, Japan;
authors.forename: Yohsuke
authors.initials: Y
authors.lastname: Bansho
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan;
authors.forename: Taro
authors.initials: T
authors.lastname: Furubayashi
authors.affiliation: Exploratory Research for Advanced Technology, Japan Science and Technology Agency, Kawaguchi-shi, Saitama 332-0012, Japan; Graduate School of Information Science and Technology, Osaka University, Suita, Osaka 565-0871, Japan ichihashi@ist.osaka-u.ac.jp tetsuyayomo@gmail.com.
authors.forename: Norikazu
authors.initials: N
authors.lastname: Ichihashi
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan; Exploratory Research for Advanced Technology, Japan Science and Technology Agency, Kawaguchi-shi, Saitama 332-0012, Japan; Graduate School of Information Science and Technology, Osaka University, Suita, Osaka 565-0871, Japan ichihashi@ist.osaka-u.ac.jp tetsuyayomo@gmail.com.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yomo
mesh_terms: D000818:Animals; D005075:Biological Evolution; D006790:Host-Parasite Interactions; D010271:Parasites / Q000235:genetics; D012313:RNA / Q000096:biosynthesis*
chemical_list: D012313:RNA
keywords: RNA replication; compartment; evolution; oscillation; parasite