title: Nonspecific positivity on the Luminex crossmatch assay for anti-human leukocyte antigen antibodies due to antibodies directed against the antibody coated beads.
abstract: Two cases are described of previously unreported false positivity on the Luminex crossmatch assay due to non HLA specific antibodies directed against the beads. In both cases the Luminex crossmatch indicated the presence of donor specific antibodies to class II HLA antigens, which was not substantiated by the clinical scenario or other assays. We could demonstrate the non specificity of these antibodies through using the same assay in a modified form where beads were unexposed to cell lysate and therefore did not carry HLA antigens at all. These cases further serve to emphasize the absolute necessity of correlating positive results with the priming history, and confirming their relevance using other platforms.
authors.affiliation: Department of Transfusion Medicine and Immunohematology, Christian Medical College, Vellore, Tamil Nadu, India.
authors.forename: M P
authors.initials: MP
authors.lastname: Chacko
authors.affiliation: Department of Transfusion Medicine and Immunohematology, Christian Medical College, Vellore, Tamil Nadu, India.
authors.forename: A
authors.initials: A
authors.lastname: Augustin
authors.affiliation: Department of Nephrology, Christian Medical College, Vellore, Tamil Nadu, India.
authors.forename: V G
authors.initials: VG
authors.lastname: David
authors.affiliation: Department of Nephrology, Christian Medical College, Vellore, Tamil Nadu, India.
authors.forename: A T
authors.initials: AT
authors.lastname: Valson
authors.affiliation: Department of Transfusion Medicine and Immunohematology, Christian Medical College, Vellore, Tamil Nadu, India.
authors.forename: D
authors.initials: D
authors.lastname: Daniel
keywords: Crossmatch; Luminex; false positivity; nonhuman leukocyte antigen antibody; transplant