title: The structure of O-polysaccharides isolated from plant pathogenic bacteria Pectobacterium wasabiae IFB5408 and IFB5427.
abstract: O-Polysaccharides were isolated from the lipopolysaccharides of two strains of plant pathogenic bacteria Pectobacterium wasabiae isolated in Poland in 2013 (IFB5408 and IFB5427). The purified polysaccharides were analyzed using 1D and 2D NMR spectroscopy ((1)H, DQF-COSY, TOCSY, ROESY, HSQC, HSQC-TOCSY, and HMBC) and the chemical methods. Sugar and methylation analyses of native polysaccharides, absolute configuration assignment of constituent monosaccharides together with NMR spectroscopy data revealed that the chemical structures of both O-polysaccharides are the same.
authors.affiliation: Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Ossowska
authors.affiliation: Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Czerwicka
authors.affiliation: Department of Biotechnology, Intercollegiate Faculty of Biotechnology University of Gdansk & Medical University of Gdansk, Abrahama 58, 80-307 Gdansk, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Sledz
authors.affiliation: Department of Biotechnology, Intercollegiate Faculty of Biotechnology University of Gdansk & Medical University of Gdansk, Abrahama 58, 80-307 Gdansk, Poland.
authors.forename: Sabina
authors.initials: S
authors.lastname: Zoledowska
authors.affiliation: Department of Biotechnology, Intercollegiate Faculty of Biotechnology University of Gdansk & Medical University of Gdansk, Abrahama 58, 80-307 Gdansk, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Motyka
authors.affiliation: Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Szulta
authors.affiliation: Department of Biotechnology, Intercollegiate Faculty of Biotechnology University of Gdansk & Medical University of Gdansk, Abrahama 58, 80-307 Gdansk, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Lojkowska
authors.affiliation: Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland. Electronic address: zbigniew.kaczynski@ug.edu.pl.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Kaczyński
mesh_terms: D002236:Carbohydrate Conformation; D009682:Magnetic Resonance Spectroscopy; D044043:Pectobacterium / Q000737:chemistry*; D010935:Plant Diseases / Q000382:microbiology*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D012015:Reference Standards
chemical_list: D011134:Polysaccharides
keywords: NMR; O-polysaccharide; Pectobacterium wasabiae; Structure