title: miR-601 is a prognostic marker and suppresses cell growth and invasion by targeting PTP4A1 in breast cancer.
abstract: MicroRNAs (miRNA) play important roles in the initiation and progression of breast cancer. Here, we investigated the role of miR-601 in breast cancer and found that its expression was significantly down-regulated in breast cancer tissues compared with matched adjacent non-cancerous breast tissues. Moreover, we found that down-regulation of miR-601 was closely associated with distant metastasis and poor distant metastasis-free survival in breast cancer. In addition, miR-601 levels were inversely correlated with metastatic potential of human breast cancer cell lines. Further experiments showed that ectopic overexpression of miR-601 suppressed breast cancer cell proliferation, migration and invasion, whereas miR-601 knockdown promoted breast cancer cell proliferation, migration and invasion. Furthermore, protein tyrosine phosphatase type IVA 1 (PTP4A1) was identified as a direct target of miR-601. Overexpression of miR-601 repressed PTP4A1 mRNA and protein expression. Conversely, inhibition of miR-601 increased PTP4A1 mRNA and protein expression. Taken together, our data suggest that miR-601 inhibits growth and invasion of breast cancer cells by targeting PTP4A1 and that miR-601 is a potential biomarker for prognosis and therapeutic target in breast cancer.
authors.affiliation: Department of Basic Medicine, Guiyang College of Traditional Chinese Medicine, Guiyang 550002, People's Republic of China.
authors.forename: Jing-Ye
authors.initials: JY
authors.lastname: Hu
authors.affiliation: Department of Radiation Oncology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yi
authors.affiliation: Department of Radiation Oncology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, People's Republic of China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou 510060, People's Republic of China.
authors.forename: Mei-Yin
authors.initials: MY
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou 510060, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou 510060, People's Republic of China.
authors.forename: Li-Si
authors.initials: LS
authors.lastname: Zeng
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, People's Republic of China.
authors.forename: Zhi-Jie
authors.initials: ZJ
authors.lastname: Huang
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, People's Republic of China. Electronic address: hychenjs@126.com.
authors.forename: Jing-Song
authors.initials: JS
authors.lastname: Chen
mesh_terms: D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D018572:Disease-Free Survival; D015536:Down-Regulation; D005260:Female; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D011379:Prognosis; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D014410:Tumor Stem Cell Assay
chemical_list: D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; C580741:MIRN601 microRNA, human; D008565:Membrane Proteins; D035683:MicroRNAs; C490198:PTP4A1 protein, human; D017027:Protein Tyrosine Phosphatases
keywords: Breast cancer; Metastasis; PTP4A1; Prognosis; miR-601