title: Jiulongibacter sediminis gen. nov., sp. nov., a new member of the family Cytophagaceae, isolated from the surface sediment of the Jiulong River in China.
abstract: A bacterial strain, designated JN14-9T, was isolated from surface sediment of the Jiulong River and characterized in a taxonomic study using a polyphasic approach. Strain JN14-9T was Gram-stain-negative, non-motile, rod-shaped and orange-pigmented. It can grow at 15-37 °C (optimum 25-30 °C), at pH 7-8 (optimum pH 7) and in 2-6 % (w/v) NaCl (optimum 3-4 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain JN14-9T formed an independent lineage related to the family Cytophagaceae with low 16S rRNA gene sequence similarities (<92.5 %) to members of other genera with validly published names. The principal fatty acids of strain JN14-9T were summed feature 3 (C16:1ω6c/C16:1ω7c) and iso-C15:0. The isoprenoid quinone was identified as MK-7. The major polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, three aminophospholipids and five unidentified phospholipids. The DNA G+C content was 41.6 mol%. Results of phenotypic, phylogenetic and chemotaxonomic analyses clearly indicate that strain JN14-9T represents a novel species of a new genus within the family Cytophagaceae, for which the name Jiulongibacter sediminis gen. nov., sp. nov. is proposed. The type strain of the type species is JN14-9T (=MCCC 1A00733T=KCTC 42153T).
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, The Third Institute of State Oceanic Administration; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Centre; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, The Third Institute of State Oceanic Administration; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Centre; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, The Third Institute of State Oceanic Administration; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Centre; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, China.
authors.forename: Qiliang
authors.initials: Q
authors.lastname: Lai
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, The Third Institute of State Oceanic Administration; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Centre; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, China.
authors.forename: Chunming
authors.initials: C
authors.lastname: Dong
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, The Third Institute of State Oceanic Administration; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Centre; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, China.
authors.forename: Yanrong
authors.initials: Y
authors.lastname: Xie
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, The Third Institute of State Oceanic Administration; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Centre; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, China.
authors.forename: Zongze
authors.initials: Z
authors.lastname: Shao
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7