title: Immunoglobulin G-dependent enhancement of the infection with Coxsackievirus B4 in a murine system.
abstract: It was demonstrated that specific IgG can enhance the infection with CV-B4, in vitro, in the human system. This enhancement could be involved in the pathophysiology of CV-B4 induced diseases. To investigate further the role of enhancing IgG in the infection with CV-B4 E2 in vivo, animal models are needed. Therefore, it was decided to assess whether inoculation of CV-B4 E2 to mice results in the appearance of IgG able to enhance the infection with this virus. Swiss albino mice were inoculated with CV-B4 E2 intraperitoneally. Serum samples were obtained from tail vein blood collected from day 0 to day 80 p.i. IgG were isolated by Protein G affinity chromatography. Seroneutralisation assays were carried out. In total murine spleen cells cultures inoculated with CV-B4 E2 mixed with various dilutions of serum or IgG samples, the enhancing activity was assayed through i) the antiviral activity titer of supernatants ii) the detection of intracellular viral RNA by RT-PCR iii) the level of infectious particles in supernatants. In most serum samples (76/105), neutralizing and enhancing activities were detected peaking between days 14 and 30 p.i and were higher in sera from mice inoculated with 2.10(6) TCID50 units than with lower doses. The enhancing activity was due to the IgG-enriched fraction of serum from CV-B4 E2 infected animals but not from control animals. These data show that IgG from immune mice can enhance the infection of splenocytes with CV-B4 E2 in vitro and open the way to explore whether such an enhancing activity can play a role in vivo.
authors.affiliation: a Université de Lille; Faculté de Médecine, CHRU de Lille, Laboratoire de Virologie/EA3610 , Lille , France.|b Université de Monastir, Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives LR99ES27, Faculté de Pharmacie de Monastir , Monastir , Tunisia.|c Université de Tunis El Manar, Faculté des Sciences de Tunis , Tunis , Tunisia.
authors.forename: Firas
authors.initials: F
authors.lastname: Elmastour
authors.affiliation: b Université de Monastir, Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives LR99ES27, Faculté de Pharmacie de Monastir , Monastir , Tunisia.|c Université de Tunis El Manar, Faculté des Sciences de Tunis , Tunis , Tunisia.
authors.forename: Hela
authors.initials: H
authors.lastname: Jaidane
authors.affiliation: a Université de Lille; Faculté de Médecine, CHRU de Lille, Laboratoire de Virologie/EA3610 , Lille , France.|b Université de Monastir, Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives LR99ES27, Faculté de Pharmacie de Monastir , Monastir , Tunisia.
authors.forename: Leila
authors.initials: L
authors.lastname: Aguech-Oueslati
authors.affiliation: a Université de Lille; Faculté de Médecine, CHRU de Lille, Laboratoire de Virologie/EA3610 , Lille , France.
authors.forename: Mehdi Ayech
authors.initials: MA
authors.lastname: Benkahla
authors.affiliation: b Université de Monastir, Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives LR99ES27, Faculté de Pharmacie de Monastir , Monastir , Tunisia.
authors.forename: Mahjoub
authors.initials: M
authors.lastname: Aouni
authors.affiliation: b Université de Monastir, Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives LR99ES27, Faculté de Pharmacie de Monastir , Monastir , Tunisia.
authors.forename: Jawhar
authors.initials: J
authors.lastname: Gharbi
authors.affiliation: a Université de Lille; Faculté de Médecine, CHRU de Lille, Laboratoire de Virologie/EA3610 , Lille , France.
authors.forename: Famara
authors.initials: F
authors.lastname: Sane
authors.affiliation: a Université de Lille; Faculté de Médecine, CHRU de Lille, Laboratoire de Virologie/EA3610 , Lille , France.
authors.forename: Didier
authors.initials: D
authors.lastname: Hober
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D019067:Antibody-Dependent Enhancement; D003384:Coxsackievirus Infections / Q000276:immunology* / Q000821:virology; D003470:Culture Media; D029822:Enterovirus B, Human / Q000276:immunology* / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D051379:Mice; D009500:Neutralization Tests; D012367:RNA, Viral / Q000302:isolation & purification; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000821:virology
chemical_list: D057134:Antibodies, Neutralizing; D003470:Culture Media; D007074:Immunoglobulin G; D012367:RNA, Viral
keywords: antiviral activity; enteroviral RNA; enterovirus; in vitro; serum; spleen cells