title: Hemato-biochemical and hormonal profiles in post-partum water buffaloes (Bubalus bubalis).
abstract: OBJECTIVE
  The objective of the present study was to compare serum as well as follicular fluid (FF) biochemical and hormonal profiles along with hematological parameters in postpartum estrus, anestrus, and cystic buffaloes.
  
  
  METHODS
  Postpartum buffaloes were selected in three different groups (within 40-60 days of parturition at estrus-Group-I, postpartum >90 days at anestrum-Group-II, and postpartum cystic buffaloes in Group III). The animals selected were examined for follicular wave dynamics by routine trans-rectal ultrasonography and FF was collected by transvaginal ultrasound-guided ovum pick up technique. All hematological and biochemical parameters were analyzed by automatic analyzers while hormonal profiles analyzed by commercially available ELISA kits.
  
  
  RESULTS
  In the present investigation, estrum and anestrum animal differ significantly in hemoglobin levels. Serum estradiol differs significantly in estrus and anestrus while no significant difference in progesterone concentration was noted among all three stages. The results of our study suggest that significant higher increase in total protein (TP), calcium and glucose values in estrum while urea, aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase significantly higher in anestrum animals.
  
  
  CONCLUSIONS
  The conclusion of the present study is that TP and albumin, calcium, urea, glucose affects oocyte development and quality.
authors.affiliation: Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.
authors.forename: Sunil
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Division of Animal Physiology and Reproduction, Central Institute for Research on Buffalo, Hisar, Haryana, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Balhara
authors.affiliation: Division of Animal Physiology and Reproduction, Central Institute for Research on Buffalo, Hisar, Haryana, India.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Kumar
authors.affiliation: Department of Biochemistry, College of Basic Sciences and Humanities, CCS Haryana Agricultural University, Haryana, India.
authors.forename: Naresh
authors.initials: N
authors.lastname: Kumar
authors.affiliation: Department of Animal Biotechnology, College of Veterinary and Animal Science, Lala Lajpat Rai University of Veterinary and Animal Sciences, Haryana, India.
authors.forename: Lukumoni
authors.initials: L
authors.lastname: Buragohain
authors.affiliation: Department of Animal Biotechnology, College of Veterinary and Animal Science, Lala Lajpat Rai University of Veterinary and Animal Sciences, Haryana, India.
authors.forename: Daoharu
authors.initials: D
authors.lastname: Baro
authors.affiliation: Division of Animal Physiology and Reproduction, Central Institute for Research on Buffalo, Hisar, Haryana, India.
authors.forename: R K
authors.initials: RK
authors.lastname: Sharma
authors.affiliation: Division of Animal Physiology and Reproduction, Central Institute for Research on Buffalo, Hisar, Haryana, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Phulia
authors.affiliation: Division of Animal Physiology and Reproduction, Central Institute for Research on Buffalo, Hisar, Haryana, India.
authors.forename: Inderjeet
authors.initials: I
authors.lastname: Singh
keywords: biochemical; buffalo; follicular fluid; hematology; ovum pick up