title: Structural Analysis of the Phenol-Responsive Sensory Domain of the Transcription Activator PoxR.
abstract: Positive phenol-degradative gene regulator (PoxR) is a σ(54)-dependent AAA+ ATPase transcription activator that regulates the catabolism of phenols. The PoxR sensory domain detects phenols and relays signals for the activation of transcription. Here we report the first structure of the phenol sensory domain bound to phenol and five derivatives. It exists as a tightly intertwined homodimer with a phenol-binding pocket buried inside, placing two C termini on the same side of the dimer. His102 and Trp130 interact with the hydroxyl group of the phenol in a cavity surrounded by rigid hydrophobic residues on one side and a flexible region on the other. Each monomer has a V4R fold with a unique zinc-binding site. A shift at the C-terminal helix suggests that there is a possible conformational change upon ligand binding. The results provide a structural basis of chemical effector binding for transcriptional regulation with broad implications for protein engineering.
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea; Department of Bio-Analytical science, University of Science and Technology, Daejeon 305-333, Korea.
authors.forename: Vinod Vikas
authors.initials: VV
authors.lastname: Patil
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea; Department of Bio-Analytical science, University of Science and Technology, Daejeon 305-333, Korea.
authors.forename: Kwang-Hyun
authors.initials: KH
authors.lastname: Park
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea; Biosystems and Bioengineering Program, University of Science and Technology, Daejeon 305-333, Korea.
authors.forename: Seung-Goo
authors.initials: SG
authors.lastname: Lee
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea; Department of Bio-Analytical science, University of Science and Technology, Daejeon 305-333, Korea. Electronic address: ejwoo@kribb.re.kr.
authors.forename: Euijeon
authors.initials: E
authors.lastname: Woo
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism*; D001665:Binding Sites; D015964:Gene Expression Regulation, Bacterial; D008958:Models, Molecular; D010636:Phenols / Q000378:metabolism*; D011485:Protein Binding; D017433:Protein Structure, Secondary
chemical_list: D001426:Bacterial Proteins; D010636:Phenols
keywords: AAA+ ATPase family; DmpR; Enhancer-binding Protein; Phenol; PoxR