title: Surface characterization and biological evaluation of silver-incorporated DLC coatings fabricated by hybrid RF PACVD/MS method.
abstract: Since the biological response of the body towards an implanted material is mainly governed by its surface properties, biomaterials are improved by various kinds of coatings. Their role is to provide good mechanical and biological characteristics, and exclude some disadvantages like post-implantation infections. This phenomenon may be reduced by introduction of silver as an antibacterial agent. This study evaluates the Ag-DLC films synthesized by the hybrid RF PACVD/MS method according to the patent number PL401955-A1 worked out inter alia by the authors. Such tests as XPS, SEM, EDS, AFM, FTIR, Raman and ICP-TOF-MS were used to determine surface properties of the coatings. The obtained results were correlated with the biological response estimated on the basis of cells viability assay (osteoblast cells line Saos-2) and bacterial colonization test (Escherichia coli strain DH5α). Results showed that the hybrid RF PACVD/MS method allows one to get tight coating preventing the diffusion of harmful elements from the metallic substrate. Ag concentration increases with the growing power density, it occurs in metallic state, does not create chemical bonds and is evenly dispersed within the DLC matrix in the form of nanoscale silver clusters. Increasing silver content above 2at.% improves bactericidal properties, but decreases cell viability.
authors.affiliation: Division of Biomedical Engineering and Functional Materials, Lodz University of Technology, Institute of Materials Science and Engineering, 1/15 Stefanowskiego St., 90-924 Lodz, Poland. Electronic address: dorota.bociaga1@gmail.com.
authors.forename: Dorota
authors.initials: D
authors.lastname: Bociąga
authors.affiliation: Division of Biophysics, Lodz University of Technology, Institute of Materials Science and Engineering, 1/15 Stefanowskiego St., 90-924 Lodz, Poland.
authors.forename: Witold
authors.initials: W
authors.lastname: Jakubowski
authors.affiliation: Division of Biophysics, Lodz University of Technology, Institute of Materials Science and Engineering, 1/15 Stefanowskiego St., 90-924 Lodz, Poland; BioNanoPark Laboratories of Lodz Regional Park of Science and Technology, 114/116 Dubois St., 93-465 Lodz, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Komorowski
authors.affiliation: Division of Coatings Engineering and Non-metallic Materials, Lodz University of Technology, Institute of Materials Science and Engineering, 1/15 Stefanowskiego St., 90-924 Lodz, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Sobczyk-Guzenda
authors.affiliation: Division of Biomedical Engineering and Functional Materials, Lodz University of Technology, Institute of Materials Science and Engineering, 1/15 Stefanowskiego St., 90-924 Lodz, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Jędrzejczak
authors.affiliation: Division of Biomedical Engineering and Functional Materials, Lodz University of Technology, Institute of Materials Science and Engineering, 1/15 Stefanowskiego St., 90-924 Lodz, Poland.
authors.forename: Damian
authors.initials: D
authors.lastname: Batory
authors.affiliation: Division of Biomedical Engineering and Functional Materials, Lodz University of Technology, Institute of Materials Science and Engineering, 1/15 Stefanowskiego St., 90-924 Lodz, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Olejnik
mesh_terms: D002244:Carbon / Q000737:chemistry; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology; D018130:Diamond / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D056951:Photoelectron Spectroscopy; D012834:Silver / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D012834:Silver; D002244:Carbon; D018130:Diamond
keywords: Ag-DLC coatings; Antibacterial properties; Biomaterial; Biomedical applications; Cell viability