title: Magnetic Ganoderma lucidum spore microspheres: A novel material to immobilize CotA multicopper oxidase for dye decolorization.
abstract: In this study, hollow microspheres were obtained from Ganoderma lucidum spores. Then the hollow microspheres were loaded with Fe3O4 nanoparticles to prepare novel magnetic spore microspheres. TEM images and X-ray diffractometry demonstrated that the Fe3O4 nanoparticles were incorporated throughout the spore microsphere. CotA multicopper oxidase was chosen as biomacromolecule to study the loading ability of the magnetic spore microspheres. The combination of the CotA enzyme with the microsphere was observed by laser scanning confocal microscope. The loaded amount of CotA on the microspheres was 75mg/g when the CotA concentration was 1.2mg/mL and the activity recovery of the immobilized CotA was 81%. The magnetic microspheres loaded with CotA, which can be easily and quickly recovered by an external magnetic field, were used for dye decolorization. After 1h decolorization, 99% of the indigo carmine has been removed by 10mg microspheres. In addition, the immobilized CotA retained 75% of activity after 10 consecutive cycles, which indicated that the magnetic spore microspheres are good support material for immobilization of the enzyme.
authors.affiliation: School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Fan
authors.affiliation: School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China. Electronic address: wangy_msn@hit.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin 150040, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhao
authors.affiliation: School of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, China.
authors.forename: Jinzhu
authors.initials: J
authors.lastname: Song
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin 150040, China.
authors.forename: Jueyu
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin 150040, China.
authors.forename: Zijing
authors.initials: Z
authors.lastname: Jin
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry*; D007203:Indigo Carmine / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008863:Microspheres; D010088:Oxidoreductases / Q000737:chemistry*; D020940:Reishi; D013172:Spores, Fungal
chemical_list: D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; D058185:Magnetite Nanoparticles; D007203:Indigo Carmine; D010088:Oxidoreductases
keywords: CotA immobilization; Dye decolorization; Fe(3)O(4) nanoparticles; Spore microsphere