title: Full-length genomic characterization and molecular evolution of canine parvovirus in China.
abstract: Canine parvovirus type 2 (CPV-2) can cause acute haemorrhagic enteritis in dogs and myocarditis in puppies. This disease has become one of the most serious infectious diseases of dogs. During 2014 in China, there were many cases of acute infectious diarrhoea in dogs. Some faecal samples were negative for the CPV-2 antigen based on a colloidal gold test strip but were positive based on PCR, and a viral strain was isolated from one such sample. The cytopathic effect on susceptible cells and the results of the immunoperoxidase monolayer assay, PCR, and sequencing indicated that the pathogen was CPV-2. The strain was named CPV-NY-14, and the full-length genome was sequenced and analysed. A maximum likelihood tree was constructed using the full-length genome and all available CPV-2 genomes. New strains have replaced the original strain in Taiwan and Italy, although the CPV-2a strain is still predominant there. However, CPV-2a still causes many cases of acute infectious diarrhoea in dogs in China.
authors.affiliation: Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Centre for Nanyang Veterinary Biological Engineering Technology, Nanyang Normal University, Nanyang, 473061, China.
authors.forename: Qinghai
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin, 150001, Heilongjiang, China.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Kong
authors.affiliation: Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Liang
authors.affiliation: Centre for Nanyang Veterinary Biological Engineering Technology, Nanyang Normal University, Nanyang, 473061, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Mao
authors.affiliation: Centre for Nanyang Veterinary Biological Engineering Technology, Nanyang Normal University, Nanyang, 473061, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Bu
authors.affiliation: Centre for Nanyang Veterinary Biological Engineering Technology, Nanyang Normal University, Nanyang, 473061, China.
authors.forename: Lunguang
authors.initials: L
authors.lastname: Yao
authors.affiliation: School of Biological Science and Technology, University of Jinan, No. 336, West Road of Nan Xinzhuang, Shizhong District, Jinan, 250022, Shandong, China. zybin395@126.com.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
authors.affiliation: Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences, Beijing, 100193, China. cuishangjin@126.com.|State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin, 150001, Heilongjiang, China. cuishangjin@126.com.
authors.forename: Shangjin
authors.initials: S
authors.lastname: Cui
authors.affiliation: Federal University of Pará, Belém, Brazil.
authors.forename: Élcio
authors.initials: É
authors.lastname: Leal
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D002874:Chromosome Mapping; D004279:DNA, Viral / Q000235:genetics; D004283:Dog Diseases / Q000453:epidemiology / Q000821:virology*; D004285:Dogs; D019143:Evolution, Molecular; D005243:Feces / Q000821:virology; D014644:Genetic Variation; D016679:Genome, Viral; D010322:Parvoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral
keywords: CPV-2; Phylogenetic analysis; Viral evolution