title: Curindolizine, an Anti-Inflammatory Agent Assembled via Michael Addition of Pyrrole Alkaloids Inside Fungal Cells.
abstract: Curvularia sp. IFB-Z10, a white croaker-associated fungus, generates a skeletally unprecedented indolizine alkaloid named curindolizine (1), which displays an anti-inflammatory action in lipopolyssacharide (LPS)-induced RAW 264.7 macrophages with an IC50 value of 5.31 ± 0.21 μM. The enzymatic transformation test demonstrated that the unique curindolizine architecture was most likely produced by the regiospecific in-cell Michael addition reaction between pyrrole alkaloids, curvulamine, and 3,5-dimethylindolizin-8(5H)-one.
authors.affiliation: Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University , Nanjing 210093, China.
authors.forename: Wen Bo
authors.initials: WB
authors.lastname: Han
authors.affiliation: Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University , Nanjing 210093, China.|State Key Laboratory of Elemento-organic Chemistry, Nankai University , Tianjin 300071, China.
authors.forename: Ai Hua
authors.initials: AH
authors.lastname: Zhang
authors.affiliation: Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University , Nanjing 210093, China.
authors.forename: Xin Zhao
authors.initials: XZ
authors.lastname: Deng
authors.affiliation: College of Chemistry & Materials Engineering, Wenzhou University , Wenzhou 325035, China.
authors.forename: Xinxiang
authors.initials: X
authors.lastname: Lei
authors.affiliation: Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University , Nanjing 210093, China.
authors.forename: Ren Xiang
authors.initials: RX
authors.lastname: Tan
mesh_terms: D000470:Alkaloids / Q000737:chemistry*; D000893:Anti-Inflammatory Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D005658:Fungi; D026121:Indole Alkaloids; D007212:Indolizines / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*
chemical_list: C000611012:3,5-dimethylindolizin-8(5H)-one; D000470:Alkaloids; D000893:Anti-Inflammatory Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D026121:Indole Alkaloids; D007212:Indolizines; D008070:Lipopolysaccharides; D011758:Pyrroles; C000599394:curvulamine