title: Shared genetic control of expression and methylation in peripheral blood.
abstract: BACKGROUND
  Expression QTLs and epigenetic marks are often employed to provide an insight into the possible biological mechanisms behind GWAS hits. A substantial proportion of the variation in gene expression and DNA methylation is known to be under genetic control. We address the proportion of genetic control that is shared between these two genomic features.
  
  
  RESULTS
  An exhaustive search for pairwise phenotypic correlations between gene expression and DNA methylation in samples from human blood (n = 610) was performed. Of the 5 × 10(9) possible pairwise tests, 0.36 % passed Bonferroni corrected p-value cutoff of 9.9 × 10(-12). We determined that the correlation structure between probe pairs was largely due to blood cell type specificity of the expression and methylation probes. Upon adjustment of the expression and methylation values for observed blood cellular composition (n = 422), the number of probe pairs which survived Bonferroni correction reduced by more than 5400 fold. Of the 614 correlated probe pairs located on the same chromosome, 75 % share at least one methylation and expression QTL at nominal 10(-5) p-value cutoff. Those probe pairs are located within 1Mbp window from each other and have a mean of absolute value of genetic correlation equal to 0.69, further demonstrating the high degree of shared genetic control.
  
  
  CONCLUSIONS
  Overall, this study demonstrates notable genetic covariance between DNA methylation and gene expression and reaffirms the importance of correcting for cell-counts in studies on non-homogeneous tissues.
authors.affiliation: Queensland Brain Institute, University of Queensland, Brisbane, QLD, Australia. konstantin.shakhbazov@gmail.com.
authors.forename: Konstantin
authors.initials: K
authors.lastname: Shakhbazov
authors.affiliation: Queensland Brain Institute, University of Queensland, Brisbane, QLD, Australia.|The Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD, Australia.|QIMR Berghofer Medical Research Institute, Royal Brisbane Hospital, Brisbane, QLD, Australia.
authors.forename: Joseph E
authors.initials: JE
authors.lastname: Powell
authors.affiliation: Queensland Brain Institute, University of Queensland, Brisbane, QLD, Australia.|Current address: MRC Integrative Epidemiology Unit and School of Social and Community Medicine, University of Bristol, Bristol, BS8 2BN, UK.
authors.forename: Gibran
authors.initials: G
authors.lastname: Hemani
authors.affiliation: Queensland Brain Institute, University of Queensland, Brisbane, QLD, Australia.
authors.forename: Anjali K
authors.initials: AK
authors.lastname: Henders
authors.affiliation: QIMR Berghofer Medical Research Institute, Royal Brisbane Hospital, Brisbane, QLD, Australia.
authors.forename: Nicholas G
authors.initials: NG
authors.lastname: Martin
authors.affiliation: Queensland Brain Institute, University of Queensland, Brisbane, QLD, Australia.|University of Queensland Diamantina Institute, Translational Research Institute, Brisbane, QLD, Australia.
authors.forename: Peter M
authors.initials: PM
authors.lastname: Visscher
authors.affiliation: QIMR Berghofer Medical Research Institute, Royal Brisbane Hospital, Brisbane, QLD, Australia.
authors.forename: Grant W
authors.initials: GW
authors.lastname: Montgomery
authors.affiliation: Queensland Brain Institute, University of Queensland, Brisbane, QLD, Australia.|University of Queensland Diamantina Institute, Translational Research Institute, Brisbane, QLD, Australia.
authors.forename: Allan F
authors.initials: AF
authors.lastname: McRae
mesh_terms: D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005786:Gene Expression Regulation; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D040641:Quantitative Trait Loci
keywords: DNA methylation; Gene expression; Genetic correlation