title: Ultrasound diagnosis in gynecological acute abdomen.
abstract: As ultrasound diagnosis is applied more frequently in gynecological acute abdomen, we carried out this study to discuss the diagnosis of endometriosis with ultrasound imaging and analyze the efficacy of microRNA used for diagnosing endometriosis and evaluating prognosis by comparing differentially expressed microRNA in subjects with or without endometriosis. Ultrasound examination results and clinical pathological examination results of 60 cases of gynecological acute abdomen were compared. Blood samples were collected from patients with endometriosis. Of 60 cases, 38 cases recurred in 20 months after surgery and the remaining 22 cases had no recurrence in the 30-month follow-up. Additionally, blood was collected from 40 patients without endometriosis as control. Then total RNA was extracted from these blood samples to determine the difference of expression of microRNA (miR-17-5p, miR-20a, miR-199a and miR-141). Compared to healthy subjects, the endometriosis patients showed significantly increased expression of miR-199a, but the expression of miR-17-5p, miR-20a and miR-141 had an obvious decrease; the differences were statistically significant (p less than 0.01). For recurred cases, miR-199 showed a remarkably high expression and miR-17-5p and miR-20a expressed significantly low.
authors.affiliation: Department of ultrasound, the Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Obstetrics and Gynecology, the Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: S H
authors.initials: SH
authors.lastname: Cui
authors.affiliation: Department of ultrasound, the Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: H Z
authors.initials: HZ
authors.lastname: Li
authors.affiliation: Department of ultrasound, the Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Q H
authors.initials: QH
authors.lastname: Li
authors.affiliation: Department of ultrasound, the Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: R
authors.initials: R
authors.lastname: Yuan
authors.affiliation: Department of ultrasound, the Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Y P
authors.initials: YP
authors.lastname: Zhang
authors.affiliation: GE Company, Zhengzhou, Henan, China.
authors.forename: T W
authors.initials: TW
authors.lastname: Zhao
mesh_terms: D000006:Abdomen, Acute / Q000150:complications / Q000000981:diagnostic imaging* / Q000235:genetics; D000328:Adult; D005260:Female; D005786:Gene Expression Regulation; D005831:Genital Diseases, Female / Q000150:complications / Q000000981:diagnostic imaging* / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D012008:Recurrence; D014463:Ultrasonography
chemical_list: D035683:MicroRNAs