title: Effective microorganism - X attenuates circulating superoxide dismutase following an acute bout of intermittent running in hot, humid conditions.
abstract: This study determined the effectiveness of antioxidant supplementation on high-intensity exercise-heat stress. Six males completed a high-intensity running protocol twice in temperate conditions (TEMP; 20.4°C), and twice in hot conditions (HOT; 34.7°C). Trials were completed following7 days supplementation with 70 ml·day(-1) effective microorganism-X (EM-X; TEMPEMX or HOTEMX) or placebo (TEMPPLA or HOTPLA). Plasma extracellular Hsp72 (eHsp72) and superoxide dismutase (SOD) were measured by ELISA. eHsp72 and SOD increased pre-post exercise (p < 0.001), with greater eHsp72 (p < 0.001) increases observed in HOT (+1.5 ng·ml(-1)) compared to TEMP (+0.8 ng·ml(-1)). EM-X did not influence eHsp72 (p > 0.05). Greater (p < 0.001) SOD increases were observed in HOT (+0.22 U·ml(-1)) versus TEMP (+0.10 U·ml(-1)) with SOD reduced in HOTEMX versus HOTPLA (p = 0.001). Physiological and perceptual responses were all greater (p < 0.001) in HOT versus TEMP conditions, with no difference followed EM-X (p > 0.05). EM-X supplementation attenuated the SOD increases following HOT, potentiating its application as an ergogenic aid to ameliorate oxidative stress.
authors.affiliation: a Applied Sport and Exercise Physiology (ASEP) Research Group, Institute of Sport and Physical Activity Research (ISPAR), Department of Sport and Exercise Sciences , University of Bedfordshire , Bedford , UK.|g ASPETAR, Qatar Orthopaedic and Sports Medicine Hospital , Athlete Health and Performance Research Centre , Doha , Qatar.
authors.forename: Lee
authors.initials: L
authors.lastname: Taylor
authors.affiliation: b Department of Biomolecular and Sport Sciences , University of Coventry , Coventry , UK.|h Department for Health , University of Bath , Claverton Down , Bath , UK.
authors.forename: Ben J
authors.initials: BJ
authors.lastname: Lee
authors.affiliation: c Centre for Sport and Exercise Science and Medicine (SESAME) , University of Brighton, Welkin Human Performance Laboratories , Denton Road, Eastbourne , UK.|i Centre for Sports Medicine and Human Performance (CSMHP) , Brunel University London , Uxbridge , UK.
authors.forename: Oliver R
authors.initials: OR
authors.lastname: Gibson
authors.affiliation: d Sport and Physical Activity Department , Edge Hill University , Ormskirk , United Kingdom.
authors.forename: Adrian W
authors.initials: AW
authors.lastname: Midgley
authors.affiliation: c Centre for Sport and Exercise Science and Medicine (SESAME) , University of Brighton, Welkin Human Performance Laboratories , Denton Road, Eastbourne , UK.
authors.forename: Peter
authors.initials: P
authors.lastname: Watt
authors.affiliation: e Endurance Research Group, School of Sport and Exercise Sciences , University of Kent , Chatham Maritime , UK.
authors.forename: Alexis
authors.initials: A
authors.lastname: Mauger
authors.affiliation: f Muscle Cellular and Molecular Physiology (MCMP) & Applied Sport and Exercise Science(ASEP) Research Groups, Institute of Sport and Physical Activity Research (ISPAR), Department of Sport and Exercise Sciences , University of Bedfordshire , Bedford , UK.
authors.forename: Paul
authors.initials: P
authors.lastname: Castle
mesh_terms: D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D015415:Biomarkers / Q000097:blood; D018592:Cross-Over Studies; D019587:Dietary Supplements; D004311:Double-Blind Method; D050884:HSP72 Heat-Shock Proteins / Q000097:blood; D018869:Heat-Shock Response / Q000187:drug effects* / Q000502:physiology; D006358:Hot Temperature / Q000009:adverse effects*; D006801:Humans; D006813:Humidity / Q000009:adverse effects*; D016014:Linear Models; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D012420:Running / Q000502:physiology*; D013482:Superoxide Dismutase / Q000097:blood*; D055815:Young Adult
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D050884:HSP72 Heat-Shock Proteins; D013482:Superoxide Dismutase
keywords: HIT; exercise; heat shock proteins (HSP); human; oxidative stress; redox balance; repeated sprint