title: Isolation of lactobacillus reuteri from Peyer's patches and their effects on sIgA production and gut microbiota diversity.
abstract: We previously reported that specific Lactobacillus reuteri colonized within mouse Peyer's patches (PP) effectively prevented high fat diet induced obesity and low-grade chronic inflammation. We further investigated the role of PP Lactobacillus reuteri on sIgA production in rats in this study.
  
  
  
  Lactobacilli were isolated from rat PP. All isolates were L. reuteri and belonged to three phenotypes according to amplified fragment length polymorphism analysis. Typical strains of two main clusters, PP1 and PP2, were used to treat control and vitamin A deficient (VAD) rats, respectively. The feeding of PP1 and PP2 affected sIgA and Lactobacillus diversity by strain-specific manner. Free sIgA was significantly increased by PP1 (p = 0.069) and PP2 (p < 0.05) in the control rats but not in the VAD rats. Only PP1 significantly changed PP Lactobacillus diversity in the control rats (p < 0.05). However, PP2 specifically changed ileal Lactobacillus diversity in both control and VAD rats. Fecal sIgA was correlated with PP Lactobacillus diversity (R(2) = 0.7958, p = 0.011).
  
  
  
  Modulation of sIgA production by PP L. reuteri of rat is dependent on vitamin A and change of Lactobacillus diversity in PP.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, PR China.|Food Nutrition and Functional Factors Research Center, School of Food Science and Technology, Jiangnan University, Wuxi, PR China.
authors.forename: Panpan
authors.initials: P
authors.lastname: Wang
authors.affiliation: Food Nutrition and Functional Factors Research Center, School of Food Science and Technology, Jiangnan University, Wuxi, PR China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Food Science, University of Massachusetts, Amherst, MA, USA.
authors.forename: Hang
authors.initials: H
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, PR China.|Food Nutrition and Functional Factors Research Center, School of Food Science and Technology, Jiangnan University, Wuxi, PR China.
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, PR China.|Food Nutrition and Functional Factors Research Center, School of Food Science and Technology, Jiangnan University, Wuxi, PR China.
authors.forename: Guo-Wei
authors.initials: GW
authors.lastname: Le
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, PR China. sunj@jiangnan.edu.cn.|Food Nutrition and Functional Factors Research Center, School of Food Science and Technology, Jiangnan University, Wuxi, PR China. sunj@jiangnan.edu.cn.
authors.forename: Jin
authors.initials: J
authors.lastname: Sun
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism; D044822:Biodiversity; D003106:Colon / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D000069196:Gastrointestinal Microbiome / Q000502:physiology*; D007070:Immunoglobulin A / Q000378:metabolism; D007778:Lactobacillus / Q000302:isolation & purification / Q000502:physiology; D052200:Limosilactobacillus reuteri / Q000235:genetics / Q000302:isolation & purification; D008297:Male; D010581:Peyer's Patches / Q000382:microbiology*; D019936:Probiotics / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D014801:Vitamin A; D014802:Vitamin A Deficiency / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D007070:Immunoglobulin A; C070683:surface layer protein A, Bacteria; D014801:Vitamin A
keywords: Lactobacillus reuteri; Peyer's patch; Secretory immunoglobulin A; Vitamin A deficiency