title: In Vitro Comparison of Ertapenem, Meropenem, and Imipenem against Isolates of Rapidly Growing Mycobacteria and Nocardia by Use of Broth Microdilution and Etest.
abstract: We compared the activities of the carbapenems ertapenem, meropenem, and imipenem against 180 isolates of rapidly growing mycobacteria (RGM) and 170 isolates of Nocardia using the Clinical and Laboratory Standards Institute (CLSI) guidelines. A subset of isolates was tested using the Etest. The rate of susceptibility to ertapenem and meropenem was limited and less than that to imipenem for the RGM. Analysis of major and minor discrepancies revealed that >90% of the isolates of Nocardia had higher MICs by the broth microdilution method than by Etest, in contrast to the lower broth microdilution MICs seen for >80% of the RGM. Imipenem remains the most active carbapenem against RGM, including Mycobacterium abscessus subsp. abscessus For Nocardia, imipenem was significantly more active only against Nocardia farcinica Although there may be utility in testing the activities of the newer carbapenems against Nocardia, their activities against the RGM should not be routinely tested. Testing by Etest is not recommended by the CLSI.
authors.affiliation: The University of Texas Health Science Center, Tyler, Texas, USA.
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Brown-Elliott
authors.affiliation: The University of Texas Health Science Center, Tyler, Texas, USA.
authors.forename: Jessica
authors.initials: J
authors.lastname: Killingley
authors.affiliation: The University of Texas Health Science Center, Tyler, Texas, USA.
authors.forename: Sruthi
authors.initials: S
authors.lastname: Vasireddy
authors.affiliation: The University of Texas Health Science Center, Tyler, Texas, USA.
authors.forename: Linda
authors.initials: L
authors.lastname: Bridge
authors.affiliation: The University of Texas Health Science Center, Tyler, Texas, USA richard.wallace@uthct.edu.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Wallace
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000077727:Ertapenem; D006801:Humans; D015378:Imipenem / Q000494:pharmacology*; D000077731:Meropenem; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009615:Nocardia / Q000187:drug effects* / Q000302:isolation & purification; D009170:Nontuberculous Mycobacteria / Q000187:drug effects* / Q000302:isolation & purification; D013845:Thienamycins / Q000494:pharmacology*; D047090:beta-Lactams / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D013845:Thienamycins; D047090:beta-Lactams; D015378:Imipenem; D000077731:Meropenem; D000077727:Ertapenem