title: Mining metagenomic whole genome sequences revealed subdominant but constant Lactobacillus population in the human gut microbiota.
abstract: The genus Lactobacillus includes over 215 species that colonize plants, foods, sewage and the gastrointestinal tract (GIT) of humans and animals. In the GIT, Lactobacillus population can be made by true inhabitants or by bacteria occasionally ingested with fermented or spoiled foods, or with probiotics. This study longitudinally surveyed Lactobacillus species and strains in the feces of a healthy subject through whole genome sequencing (WGS) data-mining, in order to identify members of the permanent or transient populations. In three time-points (0, 670 and 700 d), 58 different species were identified, 16 of them being retrieved for the first time in human feces. L. rhamnosus, L. ruminis, L. delbrueckii, L. plantarum, L. casei and L. acidophilus were the most represented, with estimated amounts ranging between 6 and 8 Log (cells g(-1) ), while the other were detected at 4 or 5 Log (cells g(-1) ). 86 Lactobacillus strains belonging to 52 species were identified. 43 seemingly occupied the GIT as true residents, since were detected in a time span of almost 2 years in all the three samples or in 2 samples separated by 670 or 700 d. As a whole, a stable community of lactobacilli was disclosed, with wide and understudied biodiversity.
authors.affiliation: Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy.
authors.forename: Maddalena
authors.initials: M
authors.lastname: Rossi
authors.affiliation: Cavanilles Institute of Biodiversity and Evolutionary Biology, University of Valencia, Valencia, Spain.|Foundation for the Promotion of Health and Biomedical Research in the Valencian Community (FISABIO), Valencia, Spain.|Network Research Center for Epidemiology and Public Health (CIBER-ESP), Madrid, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Martínez-Martínez
authors.affiliation: Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Amaretti
authors.affiliation: Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Ulrici
authors.affiliation: Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Raimondi
authors.affiliation: Cavanilles Institute of Biodiversity and Evolutionary Biology, University of Valencia, Valencia, Spain.|Foundation for the Promotion of Health and Biomedical Research in the Valencian Community (FISABIO), Valencia, Spain.|Network Research Center for Epidemiology and Public Health (CIBER-ESP), Madrid, Spain.
authors.forename: Andrés
authors.initials: A
authors.lastname: Moya
mesh_terms: D058491:Bacterial Load; D041981:Gastrointestinal Tract / Q000382:microbiology*; D006801:Humans; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics*; D008137:Longitudinal Studies; D056186:Metagenomics; D064307:Microbiota