title: Development and evaluation of an immunochromatographic assay using a gp51 monoclonal antibody for the detection of antibodies against the bovine leukemia virus.
abstract: Infection of cattle with bovine leukemia virus (BLV) has been observed and reported worldwide, including in Korea. The onsite identification of infected cattle would help decreasing and eradicating BLV infections on farms. Here, we present a new immunochromatographic assay that employs monoclonal antibodies (MAbs) for the detection of antibodies against BLV in the field. BLV envelope glycoprotein (gp)51 was expressed inE. coli, and MAbs against recombinant BLV gp51 were generated for the development of an immunochromatographic assay to detect BLV antibodies in cattle. The sensitivity and specificity of the assay were determined by comparing these results with those obtained from a standard enzyme linked immunosorbent assay (ELISA). A total of 160 bovine sera were used to evaluate the new immunochromatographic assay. Using ELISA as a reference standard, the relative specificity and sensitivity of this assay were determined to be 94.7% and 98%, respectively. Because of its high sensitivity and specificity, this BLV antibody detection assay would be suitable for the onsite identification of BLV infection in the field.
authors.affiliation: Division of Viral Disease, Animal and Plant Quarantine Agency, Anyang 14086, Korea.
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Kim
authors.affiliation: Research Institution, MEDIAN Diagnostics Inc., Chuncheon 24399, Korea.
authors.forename: Kwang-Myun
authors.initials: KM
authors.lastname: Cheong
authors.affiliation: Division of Viral Disease, Animal and Plant Quarantine Agency, Anyang 14086, Korea.
authors.forename: Ha-Kyung
authors.initials: HK
authors.lastname: Joung
authors.affiliation: Division of Viral Disease, Animal and Plant Quarantine Agency, Anyang 14086, Korea.
authors.forename: Bo-Hye
authors.initials: BH
authors.lastname: Kim
authors.affiliation: Division of Veterinary Drugs and Biologics, Animal and Plant Quarantine Agency, Anyang 14086, Korea.
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Song
authors.affiliation: Division of Viral Disease, Animal and Plant Quarantine Agency, Anyang 14086, Korea.
authors.forename: In-Soo
authors.initials: IS
authors.lastname: Cho
authors.affiliation: Division of Animal Disease Diagnostic, Animal and Plant Quarantine Agency, Anyang 14086, Korea.
authors.forename: Kyoung-Ki
authors.initials: KK
authors.lastname: Lee
authors.affiliation: Division of Viral Disease, Animal and Plant Quarantine Agency, Anyang 14086, Korea.
authors.forename: Yeun-Kyung
authors.initials: YK
authors.lastname: Shin
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D002417:Cattle; D002846:Chromatography, Affinity / Q000662:veterinary*; D016583:Enzootic Bovine Leukosis / Q000175:diagnosis* / Q000821:virology; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D008403:Mass Screening / Q000662:veterinary*; D010865:Pilot Projects; D056910:Republic of Korea
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral
keywords: antibody detection; bovine leukemia virus; immunochromatography