title: Potential Start Codon Targeted (SCoT) and Inter-retrotransposon Amplified Polymorphism (IRAP) Markers for Evaluation of Genetic Diversity and Conservation of Wild Pistacia Species Population.
abstract: Wild pistachio species is important species in forests regions Iran and provide protection wind and soil erosion. Even though cultivation and utilization of Pistacia are fully exploited, the evolutionary history of the Pistacia genus and the relationships among the species and accessions is still not well understood. Two molecular marker strategies, SCoT and IRAP markers were analyzed for assessment of 50 accessions of this species accumulated from diverse geographical areas of Iran. A thorough of 115 bands were amplified using eight IRAP primers, of which 104 (90.4 %) have been polymorphic, and 246 polymorphic bands (68.7 %) had been located in 358 bands amplified by way of forty-four SCoT primers. Average PIC for IRAP and SCoT markers became 0.32 and 0.48, respectively. This is exposed that SCoT markers have been extra informative than IRAP for the assessment of variety among pistachio accessions. Primarily based on the two extraordinary molecular markers, cluster evaluation revealed that the 50 accessions taken for the evaluation may be divided into three distinct clusters. Those results recommend that the performance of SCoT and IRAP markers was highly the equal in fingerprinting of accessions. The results affirmed a low genetic differentiation among populations, indicating the opportunity of gene drift most of the studied populations. These findings might render striking information in breeding management strategies for genetic conservation and cultivar improvement.
authors.affiliation: Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahid Chamran University of Ahvaz, P.O.Box 61355/144, Ahvaz, Iran. karimsorkheh@gmail.com.
authors.forename: Karim
authors.initials: K
authors.lastname: Sorkheh
authors.affiliation: Department of seed and Plant improvement, Agricultural & Natural Resources Research and Education Center, P.O.Box 61333-3341, Ahvaz, Iran.
authors.forename: Nazanin
authors.initials: N
authors.lastname: Amirbakhtiar
authors.affiliation: Department of Horticulture, Agricultural Faculty, Ataturk University, 25240, Erzurum, Turkey.
authors.forename: Sezai
authors.initials: S
authors.lastname: Ercisli
mesh_terms: D018387:Codon, Initiator; D016172:DNA Fingerprinting / Q000379:methods*; D018744:DNA, Plant / Q000235:genetics; D019143:Evolution, Molecular; D040961:Genetic Drift; D010802:Phylogeny; D027927:Pistacia / Q000145:classification* / Q000235:genetics; D011110:Polymorphism, Genetic; D018626:Retroelements
chemical_list: D018387:Codon, Initiator; D018744:DNA, Plant; D018626:Retroelements
keywords: Conservation; Genetic variation; IRAP; SCoT