title: Association between rs1761667 polymorphism of CD36 gene and risk of coronary atherosclerosis in Egyptian population.
abstract: BACKGROUND
  Recent studies have demonstrated that CD36 is involved in the progression of atherosclerosis. Associations between rs1761667 polymorphisms of the CD36 gene and susceptibility to coronary artery disease (CAD) are not obvious.
  
  
  METHODS
  We studied the relationship between single nucleotide polymorphisms (SNPs), rs1761667 of CD36 gene and the risk of coronary atherosclerosis in a case-control study composed of 71 CAD patients and 76 healthy controls by assessment of allele frequencies and genotype distributions using real-time polymerase chain reaction (PCR) and the allele discrimination technique. Additionally, we detected CD36 expression by flow cytometry.
  
  
  RESULTS
  The distribution of rs1761667 genotypes between the two groups was significantly different (P<0.001), with the frequency of the AG genotype being significantly higher in the CAD group than in the control group (P<0.001). The expression level of CD36 in the CAD group was significantly higher than that in the control group (P<0.001), with significant differences in the CAD patients with an AG genotype compared with those with an AA and GG genotype (P<0.001). The plasma levels (mg/dL) of low-density lipoprotein (LDL) in the CAD group were much higher than that in the control group (P<0.001). On the other hand, the plasma LDL levels in CAD patients with the AG genotype were remarkably higher than those with the GG and AA genotypes (P=0.046) and AG genotype was significantly more prevalent among type 2 diabetes mellitus (T2DM) and metabolic syndrome (MetS) patients (P<0.05). After adjusted logistic regression analysis, the AG genotype of rs1761667 was associated with an increased risk of CAD (OR=17.97, 95% CI, 3.19-87.85, P=0.001).
  
  
  CONCLUSIONS
  The AG genotype of the rs1761667 polymorphism in the CD36 gene may be involved in CAD pathogenesis as well as increased body mass index (BMI), T2DM and MetS in the Sohag population of Egypt.
authors.affiliation: 1 Department of Internal Medicine, Cardiology Division, Cathlab Unit, Sohag University Hospital, Sohag, Egypt ; 2 Department of Internal Medicine, 3 Department of Clinical pathology, 4 Department of Internal Medicine, Hematology Division, Faculty of Medicine, Sohag University Hospital, Sohag, Egypt.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Boghdady
authors.affiliation: 1 Department of Internal Medicine, Cardiology Division, Cathlab Unit, Sohag University Hospital, Sohag, Egypt ; 2 Department of Internal Medicine, 3 Department of Clinical pathology, 4 Department of Internal Medicine, Hematology Division, Faculty of Medicine, Sohag University Hospital, Sohag, Egypt.
authors.forename: Usama Ahmed
authors.initials: UA
authors.lastname: Arafa
authors.affiliation: 1 Department of Internal Medicine, Cardiology Division, Cathlab Unit, Sohag University Hospital, Sohag, Egypt ; 2 Department of Internal Medicine, 3 Department of Clinical pathology, 4 Department of Internal Medicine, Hematology Division, Faculty of Medicine, Sohag University Hospital, Sohag, Egypt.
authors.forename: Eman A
authors.initials: EA
authors.lastname: Sabet
authors.affiliation: 1 Department of Internal Medicine, Cardiology Division, Cathlab Unit, Sohag University Hospital, Sohag, Egypt ; 2 Department of Internal Medicine, 3 Department of Clinical pathology, 4 Department of Internal Medicine, Hematology Division, Faculty of Medicine, Sohag University Hospital, Sohag, Egypt.
authors.forename: Eman
authors.initials: E
authors.lastname: Salama
authors.affiliation: 1 Department of Internal Medicine, Cardiology Division, Cathlab Unit, Sohag University Hospital, Sohag, Egypt ; 2 Department of Internal Medicine, 3 Department of Clinical pathology, 4 Department of Internal Medicine, Hematology Division, Faculty of Medicine, Sohag University Hospital, Sohag, Egypt.
authors.forename: Ahmed
authors.initials: A
authors.lastname: El Sharawy
authors.affiliation: 1 Department of Internal Medicine, Cardiology Division, Cathlab Unit, Sohag University Hospital, Sohag, Egypt ; 2 Department of Internal Medicine, 3 Department of Clinical pathology, 4 Department of Internal Medicine, Hematology Division, Faculty of Medicine, Sohag University Hospital, Sohag, Egypt.
authors.forename: Mahmoud I
authors.initials: MI
authors.lastname: Elbadry
keywords: CD36 gene; Coronary atherosclerosis; low-density lipoprotein (LDL); rs1761667; single nucleotide polymorphism (SNP)