title: Rapid assessment of drug resistance of cancer cells to gefitinib and carboplatin using optical imaging.
abstract: The overall goal of this study was to evaluate optical molecular imaging approaches to determine the drug response of chemotherapy and molecular targeted agents in drug sensitive and drug resistant cell lines. The optical molecular imaging approaches selected in this study were based on changes in intracellular uptake and retention of choline and glucose molecules. The breast cancer cell lines were treated with a molecular targeted anti-EGFR therapy. The bladder cancer cell lines were treated with a conventional chemotherapy approach. Sensitivity of optical molecular imaging approach was also compared with conventional cell viability and cell growth inhibition assays. Results demonstrate that optical molecular imaging of changes in intracellular uptake of metabolites was effective in detecting drug susceptibility for both molecular targeted therapy in breast cancer cells and chemotherapy in bladder cancer cells. Between the selected metabolites for optical molecular imaging, changes in glucose metabolic activity showed higher sensitivity in discrimination between the drug sensitive and drug resistant cell lines. The results demonstrated that optical molecular imaging approaches more significantly sensitive as compared to the conventional cell viability and growth assays. Overall, the results demonstrate potential of optical molecular imaging of metabolic activity to improve sensitivity of in-vitro drug response assays.
authors.affiliation: Department of Biological and Agricultural Engineering, University of California, Davis, Davis, CA 95616, USA.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Luo
authors.affiliation: Department of Biological and Agricultural Engineering, University of California, Davis, Davis, CA 95616, USA.
authors.forename: Kiana Michelle
authors.initials: KM
authors.lastname: Samadzadeh
authors.affiliation: Department of Biological and Agricultural Engineering, University of California, Davis, Davis, CA 95616, USA; Food Science and Technology, University of California, Davis, Davis, CA 95616, USA. Electronic address: nnitin@ucdavis.edu.
authors.forename: Nitin
authors.initials: N
authors.lastname: Nitin
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D016190:Carboplatin / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D004354:Drug Screening Assays, Antitumor; D005260:Female; D000077156:Gefitinib; D006801:Humans; D061848:Optical Imaging; D011799:Quinazolines / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; D011799:Quinazolines; D016190:Carboplatin; D000077156:Gefitinib
keywords: Chemotherapy; Drug response; Metabolic activity; Molecular imaging; Molecular targeted therapy