title: Induction of Inner Ear Hair Cells from Mouse Embryonic Stem Cells In Vitro.
abstract: Inner ear hair cells (HCs) function as the primary transducers for perception of sound and balance, while a defect in their formation or their loss results in sensory deficits. In mammals, once HCs are lost, they are not regenerated, and thus, various medical strategies have been proposed for their reproduction. Although embryonic stem (ES) cells are a promising source for cell therapy, little is known about efficient generation of hair cell-like cells (HCLs) from mouse ES cells. In the present protocol, we describe a simple method for obtaining ES-derived murine HCLs (HIST2 method).
authors.affiliation: Department of Pathogen, Infection and Immunity, Nara Medical University, 840 Shijo-Cho, Kashihara, Nara, 634-8521, Japan. myoshika@naramed-u.ac.jp.
authors.forename: Masahide
authors.initials: M
authors.lastname: Yoshikawa
authors.affiliation: Department of Pathogen, Infection and Immunity, Nara Medical University, 840 Shijo-Cho, Kashihara, Nara, 634-8521, Japan. oujix@naramed-u.ac.jp.
authors.forename: Yukiteru
authors.initials: Y
authors.lastname: Ouji
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000235:genetics*; D006199:Hair Cells, Auditory, Inner / Q000166:cytology*; D051379:Mice; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology*; D012038:Regeneration
keywords: Differentiation; Embryonic stem cell; HIST2 method; Hair cells; In vitro culture; Inner ear