title: Quantitative evaluation of regularized phase retrieval algorithms on bone scaffolds seeded with bone cells.
abstract: In the field of regenerative medicine, there has been a growing interest in studying the combination of bone scaffolds and cells that can maximize newly formed bone. In-line phase-contrast x-ray tomography was used to image porous bone scaffolds (Skelite(©)), seeded with bone forming cells. This technique allows the quantification of both mineralized and soft tissue, unlike with classical x-ray micro-computed tomography. Phase contrast images were acquired at four distances. The reconstruction is typically performed in two successive steps: phase retrieval and tomographic reconstruction. In this work, different regularization methods were applied to the phase retrieval process. The application of a priori terms for heterogeneous objects enables quantitative 3D imaging of not only bone morphology, mineralization, and soft tissue formation, but also cells trapped in the pre-bone matrix. A statistical study was performed to derive statistically significant information on the different culture conditions.
authors.affiliation: Creatis, Université de Lyon, CNRS UMR5220, Inserm U1206, Université Lyon 1, INSA-Lyon, Université Jean Monnet Saint Etienne, Villeurbanne, France. European Synchrotron Radiation Facility (ESRF), Grenoble, France.
authors.forename: L
authors.initials: L
authors.lastname: Weber
authors.forename: M
authors.initials: M
authors.lastname: Langer
authors.forename: S
authors.initials: S
authors.lastname: Tavella
authors.forename: A
authors.initials: A
authors.lastname: Ruggiu
authors.forename: F
authors.initials: F
authors.lastname: Peyrin
mesh_terms: D000465:Algorithms; D001842:Bone and Bones / Q000033:anatomy & histology* / Q000166:cytology*; D006801:Humans; D021621:Imaging, Three-Dimensional; D016062:Porosity; D054457:Tissue Scaffolds / Q000737:chemistry*; D055114:X-Ray Microtomography / Q000379:methods*