title: Tetanus toxoid-loaded cationic non-aggregated nanostructured lipid particles triggered strong humoral and cellular immune responses.
abstract: In the present investigation, non-aggregated cationic and unmodified nanoparticles (TT-C-NLPs4 and TT-NLPs1) were prepared of about 49.2 ± 6.8-nm and 40.8 ± 8.3-nm, respectively. In addition, spherical shape, crystalline architecture and cationic charge were also noticed. Furthermore, integrity and conformational stability of TT were maintained in both TT-C-NLPs4 and TT-NLPs1, as evidenced by symmetrical position of bands and superimposed spectra, respectively in SDS-PAGE and circular dichroism. Cellular uptake in RAW264.7 cells indicating the concentration-dependent internalisation of nanoparticles. Qualitatively, CLSM exhibited enhanced cellular uptake of non-aggregated TT-C-NLPs4 owing to interaction with negatively charged plasma membrane and clevaloe mediated/independent endocytosis. In last, in vivo immunisation with non-aggregated TT-C-NLPs4 elicited strong humoral (anti-TT IgG) and cellular (IFN-γ) immune responses at day 42, as compared to non-aggregated TT-NLPs1 and TT-Alum following booster immunisation at day 14 and 28. Thus, non-aggregated cationic lipid nanoparticles may be a potent immune-adjuvant for parenteral delivery of weak antigens.
authors.affiliation: a Department of Pharmaceutics , Chandigarh College of Pharmacy , Mohali , Punjab , India ;
authors.forename: Amandeep
authors.initials: A
authors.lastname: Kaur
authors.affiliation: a Department of Pharmaceutics , Chandigarh College of Pharmacy , Mohali , Punjab , India ;
authors.forename: Kiran
authors.initials: K
authors.lastname: Jyoti
authors.affiliation: b Dr. B.R Ambedkar Centre for Biomedical Research, University of Delhi , Delhi , India ;
authors.forename: Shweta
authors.initials: S
authors.lastname: Rai
authors.affiliation: a Department of Pharmaceutics , Chandigarh College of Pharmacy , Mohali , Punjab , India ;
authors.forename: Rupinder
authors.initials: R
authors.lastname: Sidhu
authors.affiliation: c SLT Institute of Pharmaceutical Sciences, Guru Ghasidas University , Bilaspur , Chhattisgarh , India.
authors.forename: Ravi Shankar
authors.initials: RS
authors.lastname: Pandey
authors.affiliation: a Department of Pharmaceutics , Chandigarh College of Pharmacy , Mohali , Punjab , India ;
authors.forename: Upendra Kumar
authors.initials: UK
authors.lastname: Jain
authors.affiliation: b Dr. B.R Ambedkar Centre for Biomedical Research, University of Delhi , Delhi , India ;
authors.forename: Anju
authors.initials: A
authors.lastname: Katyal
authors.affiliation: a Department of Pharmaceutics , Chandigarh College of Pharmacy , Mohali , Punjab , India ;
authors.forename: Jitender
authors.initials: J
authors.lastname: Madan
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology; D000534:Alum Compounds / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D004337:Drug Carriers / Q000737:chemistry*; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D007114:Immunization; D008055:Lipids / Q000737:chemistry*; D008297:Male; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D000067996:RAW 264.7 Cells; D017208:Rats, Wistar; D013742:Tetanus / Q000276:immunology / Q000517:prevention & control*; D013745:Tetanus Toxoid / Q000008:administration & dosage* / Q000494:pharmacology
chemical_list: D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D004337:Drug Carriers; D008055:Lipids; D013745:Tetanus Toxoid; C041524:aluminum sulfate
keywords: Apoptosis; cellular immunity; cellular uptake; cytotoxicity; humoral immunity; non-aggregated cationic lipid nanoparticles; tetanus toxoid