title: [Features of epithelial-to-mesenchymal transition in nasal polyposis].
abstract: OBJECTIVE
  To detect the expression of epithelial-to-mesenchymal transition (EMT) biomarkers in nasal polyposis (NP) and to determine the effect of transforming growth factor β1 (TGF-β1) on EMT in cultured nasal epithelial cells.
  
  
  METHODS
  The specimens were obtained from sinus mucosa of 10 NP patients and inferior turbinate mucosa of 10 nasal septum deviation patients. The difference of mRNA expression of E-cadherin, β-catenin , zonula occludens 1 (ZO-1), vimentin and α-smooth muscle actin (α-SMA) in tissue and cultured nasal epithelial cells was detected by real-time PCR. The difference of protein exprssion of E-cadherin and vimentin in cultured nasal epithelial cells was detected by Western blot.SPSS 16.0 software was used to analyze the data.
  
  
  RESULTS
  The relative expression of E-cadherin and ZO-1 in NP tissues (0.012±0.007; 0.006±0.003) was higher than in normal nasal mucosa (0.041±0.024; 0.011±0.005), the difference was significant (t=3.675, P<0.01; t=2.956, P<0.05). However, there was no significant difference in the relative expression of β-catenin, vimentin and α-SMA between two groups (t value was 0.990, 0.429, 0.326, all P>0.05). In cultured nasal epithelial cells both from two groups, TGF-β1 induced the decreased E-cadherin, ZO-1 (tcontrol value was 3.639, 3.430, both P<0.05; tNP value was 3.279, 2.864, both P<0.05) and increased α-SMA, vimentin mRNA expression (tcontrol value was -6.393, -3.085, all P<0.05; tNP value was -2.981, -3.087, both P<0.05). Also, TGF-β1 induced the decreased E-cadherin and increased vimentin protein expression (tcontrol value was 3.583, -3.844, both P<0.05; tNP value was 5.113, -3.642, both P<0.05).
  
  
  CONCLUSIONS
  EMT is likely to contribute to nasal polyposis and TGF-β1 is involved in this process.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Tongren Hospital, Capital Medical University, Beijing 100730, China; Beijing Institute of Otorhinolaryngology, Beijing Key Labortory of Nasal Diseases, Beijing 100005, China.
authors.forename: Jingyun
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Tongren Hospital, Capital Medical University, Beijing 100730, China; Department of Allergy, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, Chian; Beijing Institute of Otorhinolaryngology, Beijing Key Labortory of Nasal Diseases, Beijing 100005, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Tongren Hospital, Capital Medical University, Beijing 100730, China; Department of Allergy, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, Chian; Beijing Institute of Otorhinolaryngology, Beijing Key Labortory of Nasal Diseases, Beijing 100005, China.
authors.forename: Luo
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000199:Actins / Q000378:metabolism; D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism; D002478:Cells, Cultured; D058750:Epithelial-Mesenchymal Transition; D006801:Humans; D009297:Nasal Mucosa / Q000378:metabolism*; D009298:Nasal Polyps / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D014746:Vimentin / Q000378:metabolism; D062826:Zonula Occludens-1 Protein / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; D015703:Antigens, CD; C513384:CDH1 protein, human; C495270:CTNNB1 protein, human; D015820:Cadherins; D012333:RNA, Messenger; C569042:TJP1 protein, human; D053773:Transforming Growth Factor beta1; D014746:Vimentin; D062826:Zonula Occludens-1 Protein; D051176:beta Catenin