title: Revelation of the IFNα, IL-10, IL-8 and IL-1β as promising biomarkers reflecting immuno-pathological mechanisms in porcine Huntington's disease model.
abstract: Studies on Huntington's disease (HD) demonstrated altered immune response in HD gene carriers. Using multiplexing immunoassay, we simultaneously investigated seven cytokines in secretomes of microglia and blood monocytes, cerebrospinal fluid (CSF) and serum collected from transgenic HD minipigs at pre-symptomatic disease stage. Decline in IFNα and IL-10 was observed in CSF and secretome of microglia whilst elevated IL-8 and IL-1β levels were secreted by microglia. Additionally, IL-8 was increased in serum. The proportion of mutant huntingtin in microglia may have causative impact on cytokine production. IFNα, IL-10, IL-8 and IL-1β represent promising biomarkers reflecting immuno-pathological mechanisms in porcine HD model.
authors.affiliation: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, v.v.i., Rumburska 89, 277 21 Libechov, Czech Republic; Research Center PIGMOD, Rumburska 89, 277 21 Libechov, Czech Republic; Department of Cell Biology, Faculty of Science, Charles University, Vinicna 7, 128 43 Prague, Czech Republic.
authors.forename: Ivona
authors.initials: I
authors.lastname: Valekova
authors.affiliation: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, v.v.i., Rumburska 89, 277 21 Libechov, Czech Republic; Research Center PIGMOD, Rumburska 89, 277 21 Libechov, Czech Republic.
authors.forename: Karla
authors.initials: K
authors.lastname: Jarkovska
authors.affiliation: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, v.v.i., Rumburska 89, 277 21 Libechov, Czech Republic; Research Center PIGMOD, Rumburska 89, 277 21 Libechov, Czech Republic; Department of Cell Biology, Faculty of Science, Charles University, Vinicna 7, 128 43 Prague, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Kotrcova
authors.affiliation: Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, CA 92008, USA.
authors.forename: John
authors.initials: J
authors.lastname: Bucci
authors.affiliation: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, v.v.i., Rumburska 89, 277 21 Libechov, Czech Republic; Research Center PIGMOD, Rumburska 89, 277 21 Libechov, Czech Republic.
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Ellederova
authors.affiliation: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, v.v.i., Rumburska 89, 277 21 Libechov, Czech Republic; Research Center PIGMOD, Rumburska 89, 277 21 Libechov, Czech Republic.
authors.forename: Stefan
authors.initials: S
authors.lastname: Juhas
authors.affiliation: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, v.v.i., Rumburska 89, 277 21 Libechov, Czech Republic; Research Center PIGMOD, Rumburska 89, 277 21 Libechov, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Motlik
authors.affiliation: Thermo Fisher Scientific - Life Science Solutions, 7335 Executive Way, Frederick, MD 21704, USA.
authors.forename: Suresh Jivan
authors.initials: SJ
authors.lastname: Gadher
authors.affiliation: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, v.v.i., Rumburska 89, 277 21 Libechov, Czech Republic; Research Center PIGMOD, Rumburska 89, 277 21 Libechov, Czech Republic. Electronic address: kovarova@iapg.cas.cz.
authors.forename: Hana
authors.initials: H
authors.lastname: Kovarova
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D015415:Biomarkers / Q000378:metabolism; D002135:Calcium-Binding Proteins; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology / Q000473:pathology; D016207:Cytokines / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000235:genetics; D006801:Humans; D000071058:Huntingtin Protein; D006816:Huntington Disease / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D016898:Interferon-alpha / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008840:Microfilament Proteins; D017628:Microglia / Q000187:drug effects / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D013552:Swine; D013556:Swine, Miniature
chemical_list: C112986:AIF1 protein, human; D015415:Biomarkers; D002135:Calcium-Binding Proteins; D016207:Cytokines; D004268:DNA-Binding Proteins; C086055:HTT protein, human; D000071058:Huntingtin Protein; D016898:Interferon-alpha; D053583:Interleukin-1beta; D016209:Interleukin-8; D008070:Lipopolysaccharides; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins; D016753:Interleukin-10
keywords: Biomarker(s); Central nervous system; Cytokines; Immune response; Porcine Huntington's disease model; Serum