title: Stem cell-derived cell-sheets for connective tissue engineering.
abstract: Cell-sheet technology involves the recovery of cells with its secreted ECM and cell-cell junctions intact, and thereby harvesting them in a single contiguous layer. Temperature changes coupled with a thermoresponsive polymer grafted culture plate surface are typically used to induce detachment of this cell-matrix layer by controlling the hydrophobicity and hydrophilicity properties of the culture surface. This review article details the genesis and development of this technique as a critical tissue-engineering tool, with a comprehensive discussion on connective tissue applications. This includes applications in the myocardial, vascular, cartilage, bone, tendon/ligament, and periodontal areas among others discussed. In particular, further focus will be given to the use of stem cells-derived cell-sheets, such as those involving bone marrow-derived and adipose tissue-derived mesenchymal stem cells. In addition, some of the associated challenges faced by approaches using stem cells-derived cell-sheets will also be discussed. Finally, recent advances pertaining to technologies forming, detaching, and manipulating cell-sheets will be covered in view of the potential impact they will have on shaping the way cell-sheet technology will be utilized in the future as a tissue-engineering technique.
authors.affiliation: a Department of Biomedical Engineering, Faculty of Engineering , National University of Singapore , Singapore.|b NUS Tissue Engineering Programme, Life Sciences Institute, National University of Singapore , Singapore.
authors.forename: Puay Yong
authors.initials: PY
authors.lastname: Neo
authors.affiliation: a Department of Biomedical Engineering, Faculty of Engineering , National University of Singapore , Singapore.|b NUS Tissue Engineering Programme, Life Sciences Institute, National University of Singapore , Singapore.
authors.forename: Thomas Kok Hiong
authors.initials: TK
authors.lastname: Teh
authors.affiliation: a Department of Biomedical Engineering, Faculty of Engineering , National University of Singapore , Singapore.
authors.forename: Alex Sheng Ru
authors.initials: AS
authors.lastname: Tay
authors.affiliation: a Department of Biomedical Engineering, Faculty of Engineering , National University of Singapore , Singapore.
authors.forename: Maria Christine Tankeh
authors.initials: MC
authors.lastname: Asuncion
authors.affiliation: a Department of Biomedical Engineering, Faculty of Engineering , National University of Singapore , Singapore.|b NUS Tissue Engineering Programme, Life Sciences Institute, National University of Singapore , Singapore.
authors.forename: Si Ning
authors.initials: SN
authors.lastname: Png
authors.affiliation: a Department of Biomedical Engineering, Faculty of Engineering , National University of Singapore , Singapore.|c Department of Mechanical Engineering, Faculty of Engineering , National University of Singapore , Singapore.
authors.forename: Siew Lok
authors.initials: SL
authors.lastname: Toh
authors.affiliation: a Department of Biomedical Engineering, Faculty of Engineering , National University of Singapore , Singapore.|b NUS Tissue Engineering Programme, Life Sciences Institute, National University of Singapore , Singapore.|d Department of Orthopaedic Surgery , Yong Loo Lin School of Medicine, National University of Singapore , Singapore.
authors.forename: James Cho-Hong
authors.initials: JC
authors.lastname: Goh
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D006801:Humans; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
keywords: Cell-sheet; cell-sheet engineering; connective tissue; stem cells derived; tissue engineering