title: Multiplexed Imaging of Protein Phosphorylation on Membranes Based on Ti(IV) Functionalized Nanopolymers.
abstract: Accurate protein phosphorylation analysis reveals dynamic cellular signaling events not evident from protein expression levels. The most dominant biochemical assay, western blotting, suffers from the inadequate availability and poor quality of phospho-specific antibodies for phosphorylated proteins. Furthermore, multiplexed assays based on antibodies are limited by steric interference between the antibodies. Here we introduce a multifunctionalized nanopolymer for the universal detection of phosphoproteins that, in combination with regular antibodies, allows multiplexed imaging and accurate determination of protein phosphorylation on membranes.
authors.affiliation: Department of Biochemistry, Purdue University, 201 S University, West Lafayette, IN, 47907, USA.|Tymora Analytical Operations, 3495 Kent Avenue, West Lafayette, IN, 47906, USA.
authors.forename: Anton
authors.initials: A
authors.lastname: Iliuk
authors.affiliation: Department of Biochemistry, Purdue University, 201 S University, West Lafayette, IN, 47907, USA.|Tymora Analytical Operations, 3495 Kent Avenue, West Lafayette, IN, 47906, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Biochemistry, Purdue University, 201 S University, West Lafayette, IN, 47907, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Melesse
authors.affiliation: Department of Biochemistry, Purdue University, 201 S University, West Lafayette, IN, 47907, USA.
authors.forename: Mark C
authors.initials: MC
authors.lastname: Hall
authors.affiliation: Department of Biochemistry, Purdue University, 201 S University, West Lafayette, IN, 47907, USA. watao@purdue.edu.|Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN, 47907, USA. watao@purdue.edu.
authors.forename: W Andy
authors.initials: WA
authors.lastname: Tao
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D015153:Blotting, Western; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D050091:Dendrimers / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D010750:Phosphoproteins / Q000032:analysis* / Q000276:immunology; D014025:Titanium / Q000737:chemistry*
chemical_list: D000906:Antibodies; D050091:Dendrimers; D005456:Fluorescent Dyes; C104700:PAMAM Starburst; D010750:Phosphoproteins; D014025:Titanium
keywords: antibodies; dendrimers; membranes; multiplexed analysis; phosphoproteins