title: EXTRACELLULAR ENZYMES OF THE MICROCYSTIS AERUGINOSA PCC 7813 STRAIN ARE INHIBITED IN THE PRESENCE OF HYDROQUINONE AND PYROGALLOL, ALLELOCHEMICALS PRODUCED BY AQUATIC PLANTS(1).
abstract: Several cyanobacterial species have a high potential to dominate in marine environments and freshwater reservoirs, and the ecological and physiological reasons for this phenomenon are not understood comprehensively. In this study, the ability of a Microcystis aeruginosa Kütz. strain to produce free dissolved enzymes was documented. We have observed that this highly toxic strain releases alkaline phosphatase, leucine aminopeptidase, and β-glucosidase into the ambient environment. Additionally, the inhibitory activity of selected phenols produced by aquatic plants on the activity of these enzymes was analyzed. The investigated compounds, pyrogallol and, to a lesser degree, hydroquinone, decreased the activity of extracellular enzymes produced by M. aeruginosa, with leucine aminopeptidase being the most sensitive to the inhibitors. The noncompetitive character of enzymatic inhibition suggests that the polyphenols produced by aquatic plants are able to influence the activity of different extracellular or membrane-bound enzymes.
authors.affiliation: Department of Plant Physiology and Development, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland Department of Plant Physiology, Jan Kochanowski University, Swietokrzyska 15, 24-406 Kielce, Poland Department of Plant Physiology and Development, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Dziga
authors.affiliation: Department of Plant Physiology and Development, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland Department of Plant Physiology, Jan Kochanowski University, Swietokrzyska 15, 24-406 Kielce, Poland Department of Plant Physiology and Development, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Goral
authors.affiliation: Department of Plant Physiology and Development, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland Department of Plant Physiology, Jan Kochanowski University, Swietokrzyska 15, 24-406 Kielce, Poland Department of Plant Physiology and Development, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Bialczyk
authors.affiliation: Department of Plant Physiology and Development, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland Department of Plant Physiology, Jan Kochanowski University, Swietokrzyska 15, 24-406 Kielce, Poland Department of Plant Physiology and Development, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Lechowski
keywords: Microcystis aeruginosa; allelopathy; extracellular enzymes; inhibition; plant polyphenols