title: Hymenobacter sedentarius sp. nov., isolated from a soil.
abstract: A novel Gram-negative and red-pinkish bacterium designated DG5B(T) was isolated from a dry soil. Cells were rods that were catalase- and oxidase-positive, and non-motile. The strain was found to grow at temperatures from 10 to 30°C (optimum 25°C) and pH 6.0-8.0, (optimum pH 7) on R2A broth. 16S rRNA gene sequence (1,452 bp) analysis of this strain identified it as a member of the genus Hymenobacter that belongs to the class Cytophagia. The highest gene sequence similarities were with Hymenobacter arizonensis OR362-8(T) (98.3%), Hymenobacter humi DG31A(T) (97.6%), and Hymenobacter glaciei VUG-A130(T) (96.6%). Strain DG5B(T) exhibited <70% DNA-DNA relatedness with H. arizonensis (34.7 ± 7.0%; reciprocally, 29.7 ± 1.2%) and H. humi (39.4 ± 4.3%; reciprocally, 39.5 ± 3.3%) as a different genomic species, and its genomic DNA G+C content was 59.8%. Strain DG5B(T) had the following chemotaxonomic characteristics: the major fatty acids are iso-C15:0, anteiso-C15:0, C16:1 ω5c, and summed feature 3 (C16:1 ω7c / C16:1 ω6c); polar lipid profile contained phosphatidylethanolamine (PE), unknown aminophospholipid (APL), unknown glycolipids (GL), unknown phospholipids (PL), and unknown polar lipids (L); the major quinone is MK-7. The absorbance peak of pigment is at 481.0 nm. Strain DG5B(T) showed low-level resistance to gamma-ray irradiation. Phenotypic, chemotaxonomic, and genotypic properties indicated that isolate DG5B(T) represents a novel species within the genus Hymenobacter for which the name Hymenobacter sedentarius sp. nov. is proposed. The type strain is DG5B(T) (=KCTC 32524(T) =JCM 19636(T)).
authors.affiliation: College of Agricultural and Life Sciences, Kyungpook National University, Daegu, 702-701, Republic of Korea.
authors.forename: Jae-Jin
authors.initials: JJ
authors.lastname: Lee
authors.affiliation: Microorganism Resources Division, National Institute of Biological Resources, Incheon, 404-107, Republic of Korea.
authors.forename: Myung-Suk
authors.initials: MS
authors.lastname: Kang
authors.affiliation: Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul, 139-774, Republic of Korea.
authors.forename: Eun Sun
authors.initials: ES
authors.lastname: Joo
authors.affiliation: College of Agricultural and Life Sciences, Kyungpook National University, Daegu, 702-701, Republic of Korea.
authors.forename: Hee-Young
authors.initials: HY
authors.lastname: Jung
authors.affiliation: Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul, 139-774, Republic of Korea. biotech@swu.ac.kr.
authors.forename: Myung Kyum
authors.initials: MK
authors.lastname: Kim
mesh_terms: D041963:Bacteroidetes / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005720:Gamma Rays; D020459:Genes, rRNA; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000032:analysis; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D066106:Seoul; D012988:Soil Microbiology; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D008055:Lipids; D012336:RNA, Ribosomal, 16S
keywords: Hymenobacter; soil; taxonomy