title: Identification and characterization of transforming growth factor β-activated kinase 1 from Litopenaeus vannamei involved in anti-bacterial host defense.
abstract: LvTAK1, a member of transforming growth factor β-activated kinase 1 (TAK1) families, has been identified from Litopenaeus vannamei in this study. The full length of LvTAK1 is 2670 bp, including a 2277 bp open reading frame (ORF) that encoded a putative protein of 758 amino acids with a calculated molecular weight of ∼83.4 kDa LvTAK1 expression was most abundant in muscles and was up-regulated in gills after LPS, Vibrio parahaemolyticus, Staphylococcus aureus, Poly (I:C) and WSSV challenge. Both in vivo and in vitro experiments indicated that LvTAK1 could activate the expression of several antimicrobial peptide genes (AMPs). In addition, the dsRNA-mediated knockdown of LvTAK1 enhanced the susceptibility of shrimps to Vibrio parahaemolyticus, a kind of Gram-negative bacteria. These results suggested LvTAK1 played important roles in anti-bacterial infection. CoIP and subcellular localization assay demonstrated that LvTAK1 could interact with its binding protein LvTAB2, a key component of IMD pathway. Moreover, over-expression of LvTAK1 in Drosophila S2 cell could strongly induce the promoter activity of Diptericin (Dpt), a typical AMP which is used to read out of the activation of IMD pathway. These findings suggested that LvTAK1 could function as a component of IMD pathway. Interestingly, with the over-expression of LvTAK1 in S2 cell, the promoter activity of Metchnikowin (Mtk), a main target gene of Toll/Dif pathway, was up-regulated over 30 times, suggesting that LvTAK1 may also take part in signal transduction of the Toll pathway. In conclusion, we provided some evidences that the involvement of LvTAK1 in the regulation of both Toll and IMD pathways, as well as innate immune against bacterial infection in shrimp.
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, PR China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, PR China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Wang
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, PR China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, PR China.
authors.forename: Haoyang
authors.initials: H
authors.lastname: Li
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, PR China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, PR China.
authors.forename: Kai
authors.initials: K
authors.lastname: Lǚ
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Qian
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, PR China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, PR China.
authors.forename: Shaoping
authors.initials: S
authors.lastname: Weng
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, PR China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, PR China; School of Marine Sciences, Sun Yat-sen University, Guangzhou, PR China; South China Sea Resource Exploitation and Protection Collaborative Innovation Center (SCS-REPIC), PR China. Electronic address: lsshjg@mail.sysu.edu.cn.
authors.forename: Jianguo
authors.initials: J
authors.lastname: He
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, PR China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, PR China; School of Marine Sciences, Sun Yat-sen University, Guangzhou, PR China; South China Sea Resource Exploitation and Protection Collaborative Innovation Center (SCS-REPIC), PR China. Electronic address: lichaozh@mail2.sysu.edu.cn.
authors.forename: Chaozheng
authors.initials: C
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005393:Fish Diseases / Q000201:enzymology* / Q000235:genetics / Q000276:immunology*; D020930:MAP Kinase Kinase Kinases / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D033561:Penaeidae / Q000201:enzymology* / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D014733:Vibrio / Q000502:physiology; D014735:Vibrio Infections / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*; D000099151:MAP Kinase Kinase Kinase 7
chemical_list: D060829:Arthropod Proteins; D018076:DNA, Complementary; D020930:MAP Kinase Kinase Kinases; D012333:RNA, Messenger; D000099151:MAP Kinase Kinase Kinase 7
keywords: Antimicrobial peptides (AMPs); Litopenaeus vannamei; TAB2; TAK1