title: Differential regulation of the expression of aquaporins 3 and 9 by Auphen and dbcAMP in the SMMC-7721 hepatocellular carcinoma cell line.
abstract: Aquaglycero-aquaporins (agAQPs) are the structural foundation of rapid water transport and they appear to participate in cancer proliferation and malignancy. AQP3 expression is increased and AQP9 expression is decreased in hepatocellular carcinoma (HCC) compared to normal liver, which suggests their possible use as targets for cancer treatment. AQP-based modifiers, such as Auphen and dibutyryladenosine 3', 5'-cyclic monophosphate (dbcAMP), might be used to treat several diseases and as chemical tools for assessing the functions of AQPs in biological systems. We investigated the effects of both Auphen on AQP3 and dbcAMP on AQP9 in SMMC-7721 cells. We used western blotting, real-time quantitative polymerase chain reaction (qPCR) and immunohistochemistry to evaluate changes in AQP3 and AQP9 expression in SMMC-7721 cells after culturing with Auphen and dbcAMP, respectively. We also determined the proliferation of SMMC-7721 cells. We found that compared to HL-7702 (L02) liver cells, Auphen increased AQP3 expression in tumor cells, whereas dbcAMP decreased expression of AQP9 in these cells. Also, high concentrations of Auphen and dbcAMP inhibited proliferation of SMMC-7721 cells in vitro. Auphen and dbcAMP may inhibit HCC development and could be considered targets for HCC diagnosis and therapy.
authors.affiliation: a Department of Gastroenterology , Zhongshan Hospital, Fudan University , Shanghai , China.
authors.forename: R
authors.initials: R
authors.lastname: Peng
authors.affiliation: a Department of Gastroenterology , Zhongshan Hospital, Fudan University , Shanghai , China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: a Department of Gastroenterology , Zhongshan Hospital, Fudan University , Shanghai , China.
authors.forename: G X
authors.initials: GX
authors.lastname: Zhao
authors.affiliation: a Department of Gastroenterology , Zhongshan Hospital, Fudan University , Shanghai , China.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.affiliation: a Department of Gastroenterology , Zhongshan Hospital, Fudan University , Shanghai , China.
authors.forename: X Z
authors.initials: XZ
authors.lastname: Shen
authors.affiliation: a Department of Gastroenterology , Zhongshan Hospital, Fudan University , Shanghai , China.
authors.forename: J Y
authors.initials: JY
authors.lastname: Wang
authors.affiliation: a Department of Gastroenterology , Zhongshan Hospital, Fudan University , Shanghai , China.
authors.forename: J Y
authors.initials: JY
authors.lastname: Sun
mesh_terms: D051400:Aquaporin 3 / Q000378:metabolism*; D020346:Aquaporins / Q000378:metabolism*; D003994:Bucladesine / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D050607:Organogold Compounds / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: C496027:AQP3 protein, human; C109669:AQP9 protein, human; D020346:Aquaporins; D050607:Organogold Compounds; D012333:RNA, Messenger; C000613556:dichloro(phenanthroline)gold(III); D051400:Aquaporin 3; D003994:Bucladesine
keywords: Auphen; HL-7702 cells; SMMC-7721 cells; aquaglycero-aquaporins; aquaporin; cancer; dbcAMP; dibutyryladenosine 3′,5′-cyclic monophosphate; hepatocellular carcinoma