title: Systemic ocular antigen immunization leads only to a minor secondary immune response.
abstract: The immunization with optic nerve homogenate antigen (ONA) or S100 induced retinal degeneration. Since many neurological diseases are reinforced or initiated by immune cells, leucocytes were analyzed. CD3(+) T-cells in the retina increased slightly in ONA rats, but not in S100 treated retinas. No CD45R(+) B-cells and granulocytes could be detected in the retinas. At early stages, CD3(+) cells, Iba1(+) macrophages and granulocytes of the secondary lymphoid organs were not affected. Yet, the sole injection of pertussis toxin led to a shift to fewer CD45R(+) cells and more granulocytes in spleens. Later, splenic Iba1(+) macrophages were increased in both groups. We conclude that the retinal infiltration of lymphocytes is not crucial for the degeneration process and rather an epiphenomenon.
authors.affiliation: Experimental Eye Research Institute, Ruhr-University Eye Hospital, In der Schornau 23-25, 44892 Bochum, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Kuehn
authors.affiliation: Experimental Eye Research Institute, Ruhr-University Eye Hospital, In der Schornau 23-25, 44892 Bochum, Germany.
authors.forename: Mathias
authors.initials: M
authors.lastname: Stellbogen
authors.affiliation: Experimental Eye Research Institute, Ruhr-University Eye Hospital, In der Schornau 23-25, 44892 Bochum, Germany.
authors.forename: Rozina
authors.initials: R
authors.lastname: Noristani
authors.affiliation: Experimental Pneumology, Ruhr-University Bochum, Universitätsstr. 150, 44801 Bochum, Germany.
authors.forename: Marcus
authors.initials: M
authors.lastname: Peters
authors.affiliation: Experimental Eye Research Institute, Ruhr-University Eye Hospital, In der Schornau 23-25, 44892 Bochum, Germany.
authors.forename: H Burkhard
authors.initials: HB
authors.lastname: Dick
authors.affiliation: Experimental Eye Research Institute, Ruhr-University Eye Hospital, In der Schornau 23-25, 44892 Bochum, Germany. Electronic address: stephanie.joachim@rub.de.
authors.forename: Stephanie C
authors.initials: SC
authors.lastname: Joachim
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology*; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002417:Cattle; D002465:Cell Movement / Q000276:immunology; D006098:Granulocytes / Q000276:immunology; D007114:Immunization; D008198:Lymph Nodes / Q000166:cytology; D008264:Macrophages / Q000378:metabolism; D008297:Male; D008840:Microfilament Proteins / Q000378:metabolism; D009900:Optic Nerve / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew; D012160:Retina / Q000166:cytology; D012165:Retinal Ganglion Cells / Q000276:immunology; D009418:S100 Proteins / Q000494:pharmacology; D013154:Spleen / Q000166:cytology; D013997:Time Factors; D050818:Transcription Factor Brn-3 / Q000378:metabolism
chemical_list: C491049:Aif1 protein, mouse; D015703:Antigens, CD; D002135:Calcium-Binding Proteins; D008840:Microfilament Proteins; D009418:S100 Proteins; D050818:Transcription Factor Brn-3
keywords: FACS; Glaucoma; Granulocytes; Lymphocytes; Macrophages; Retina degeneration