title: G protein γ subunit 7 induces autophagy and inhibits cell division.
abstract: GNG7 (G protein γ subunit 7), a subunit of heterotrimeric G protein, is ubiquitously expressed in multiple tissues but is down-regulated in various cancers. Its expression could reduce tumor volume in mice but the mechanism was not clear. Here we show that GNG7 overexpression inhibits cell proliferation and increases cell death. GNG7 level is cell cycle-dependent and it regulates actin cytoskeleton and cell division. In addition, GNG7 is an autophagy inducer, which is the first reported Gγ protein involved in autophagy. GNG7 knockdown reduces Rapamycin and starvation-induced autophagy. Further analysis reveals that GNG7 inhibits MTOR in cells, a central regulator for autophagy and cell proliferation. In conclusion, GNG7 inhibits MTOR pathway to induce autophagy and cell death, inhibits cell division by regulating actin cytoskeleton. These combined effects lead to the antitumor capacity of GNG7.
authors.affiliation: High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei, Anhui, 230031, P. R. China.|University of Science and Technology of China, Hefei, Anhui, 230036, P. R. China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Liu
authors.affiliation: High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei, Anhui, 230031, P. R. China.
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Ji
authors.affiliation: High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei, Anhui, 230031, P. R. China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Li
authors.affiliation: High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei, Anhui, 230031, P. R. China.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Yang
authors.affiliation: High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei, Anhui, 230031, P. R. China.
authors.forename: Wenchao
authors.initials: W
authors.lastname: Wang
authors.affiliation: High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei, Anhui, 230031, P. R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001343:Autophagy; D016923:Cell Death / Q000235:genetics*; D002455:Cell Division / Q000235:genetics*; D049109:Cell Proliferation; D044388:GTP-Binding Protein gamma Subunits / Q000235:genetics*; D006801:Humans; D051379:Mice; D014162:Transfection
chemical_list: D044388:GTP-Binding Protein gamma Subunits
keywords: GNG7 (G protein γ subunit 7); actin; autophagy; cell death; cell division