title: Upregulation of 3-MST Relates to Neuronal Autophagy After Traumatic Brain Injury in Mice.
abstract: 3-mercaptopyruvate sulfurtransferase (3-MST) was a novel hydrogen sulfide (H2S)-synthesizing enzyme that may be involved in cyanide degradation and in thiosulfate biosynthesis. Over recent years, considerable attention has been focused on the biochemistry and molecular biology of H2S-synthesizing enzyme. In contrast, there have been few concerted attempts to investigate the changes in the expression of the H2S-synthesizing enzymes with disease states. To investigate the changes of 3-MST after traumatic brain injury (TBI) and its possible role, mice TBI model was established by controlled cortical impact system, and the expression and cellular localization of 3-MST after TBI was investigated in the present study. Western blot analysis revealed that 3-MST was present in normal mice brain cortex. It gradually increased, reached a peak on the first day after TBI, and then reached a valley on the third day. Importantly, 3-MST was colocalized with neuron. In addition, Western blot detection showed that the first day post injury was also the autophagic peak indicated by the elevated expression of LC3. Importantly, immunohistochemistry analysis revealed that injury-induced expression of 3-MST was partly colabeled by LC3. However, there was no colocalization of 3-MST with propidium iodide (cell death marker) and LC3 positive cells were partly colocalized with propidium iodide. These data suggested that 3-MST was mainly located in living neurons and may be implicated in the autophagy of neuron and involved in the pathophysiology of brain after TBI.
authors.affiliation: Institute of Forensic Sciences, Soochow University, Suzhou, 215123, Jiangsu, China. mingyangzhang@suda.edu.cn.|Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, Shanghai, 200063, China. mingyangzhang@suda.edu.cn.
authors.forename: Mingyang
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, North District of Suzhou Municipal Hospital, Suzhou, 215008, Jiangsu, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Shan
authors.affiliation: Central Laboratory, Second Affiliated Hospital of Xi'an Medical College, Xi'an, 710038, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Chang
authors.affiliation: Institute of Forensic Sciences, Soochow University, Suzhou, 215123, Jiangsu, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Ma
authors.affiliation: Institute of Forensic Sciences, Soochow University, Suzhou, 215123, Jiangsu, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chu
authors.affiliation: Institute of Forensic Sciences, Soochow University, Suzhou, 215123, Jiangsu, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Shen
authors.affiliation: Institute of Forensic Sciences, Soochow University, Suzhou, 215123, Jiangsu, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Institute of Forensic Sciences, Soochow University, Suzhou, 215123, Jiangsu, China.
authors.forename: Zufeng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Institute of Forensic Sciences, Soochow University, Suzhou, 215123, Jiangsu, China.
authors.forename: Chengliang
authors.initials: C
authors.lastname: Luo
authors.affiliation: Institute of Forensic Sciences, Soochow University, Suzhou, 215123, Jiangsu, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Institute of Forensic Sciences, Soochow University, Suzhou, 215123, Jiangsu, China.
authors.forename: Xiping
authors.initials: X
authors.lastname: Chen
authors.affiliation: Institute of Forensic Sciences, Soochow University, Suzhou, 215123, Jiangsu, China.
authors.forename: Luyang
authors.initials: L
authors.lastname: Tao
mesh_terms: D000818:Animals; D001343:Autophagy / Q000502:physiology*; D000070642:Brain Injuries, Traumatic / Q000201:enzymology* / Q000473:pathology; D008297:Male; D051379:Mice; D009474:Neurons / Q000201:enzymology* / Q000473:pathology; D013466:Sulfurtransferases / Q000096:biosynthesis*; D015854:Up-Regulation / Q000502:physiology*
chemical_list: D013466:Sulfurtransferases; C021550:3-mercaptopyruvate sulphurtransferase
keywords: 3-mercaptopyruvate sulfurtransferase; Autophagic cell death; Mice; Traumatic brain injury