title: Structures and genetics of biosynthesis of glycerol 1-phosphate-containing O-polysaccharides of Escherichia coli O28ab, O37, and O100.
abstract: O-polysaccharides of E. coli O28ab, O37, and O100 were found to contain glycerol 1-phosphate and the following structures of their oligosaccharide repeats were established by sugar analysis, Smith degradation (for O28ab), 1D and 2D (1)H, (13)C, and (13)P NMR spectroscopy: [Formula: see text]. Functions of putative glycosyltransferases genes in the O-antigen gene clusters of the strains studied were tentatively assigned based on similarities to genes of other E. coli O-serogroups available from GenBank and taking into account the O-polysaccharide structures established.
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, China.
authors.forename: Baopeng
authors.initials: B
authors.lastname: Yang
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Sofya N
authors.initials: SN
authors.lastname: Senchenkova
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Perepelov
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. Electronic address: yknirel@gmail.com.
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D002236:Carbohydrate Conformation; D004926:Escherichia coli / Q000737:chemistry* / Q000145:classification / Q000235:genetics*; D005994:Glycerophosphates / Q000096:biosynthesis* / Q000737:chemistry; D011134:Polysaccharides / Q000096:biosynthesis* / Q000737:chemistry*
chemical_list: D005994:Glycerophosphates; D011134:Polysaccharides; C029620:alpha-glycerophosphoric acid
keywords: Bacterial polysaccharide structure; Escherichia coli; Glycerol 1-phosphate; Lipopolysaccharide; O-antigen gene cluster; O-polysaccharide