title: Predicting human intestinal absorption in the presence of bile salt with micellar liquid chromatography.
abstract: Understanding intestinal absorption for pharmaceutical compounds is vital to estimate the bioavailability and therefore the in vivo potential of a drug. This study considers the application of micellar liquid chromatography (MLC) to predict passive intestinal absorption with a selection of model compounds. MLC is already known to aid prediction of absorption using simple surfactant systems; however, with this study the focus was on the presence of a more complex, bile salt surfactant, as would be encountered in the in vivo environment. As a result, MLC using a specific bile salt has been confirmed as an ideal in vitro system to predict the intestinal permeability for a wide range of drugs, through the development of a quantitative partition-absorption relationship. MLC offers many benefits including environmental, economic, time-saving and ethical advantages compared with the traditional techniques employed to obtain passive intestinal absorption values. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: School of Applied Sciences, University of Huddersfield, Queensgate, Huddersfield, HD1 3DH, UK.
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Waters
authors.affiliation: School of Applied Sciences, University of Huddersfield, Queensgate, Huddersfield, HD1 3DH, UK.
authors.forename: Dina S
authors.initials: DS
authors.lastname: Shokry
authors.affiliation: School of Applied Sciences, University of Huddersfield, Queensgate, Huddersfield, HD1 3DH, UK.
authors.forename: Gareth M B
authors.initials: GM
authors.lastname: Parkes
mesh_terms: D001647:Bile Acids and Salts / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D007408:Intestinal Absorption; D008823:Micelles
chemical_list: D001647:Bile Acids and Salts; D008823:Micelles
keywords: MLC; bile salts; chromatography; intestinal absorption; micellar