title: Lysobacter pocheonensis sp. nov., isolated from soil of a ginseng field.
abstract: A Gram-staining-negative, non-spore-forming, non-flagellated, rod-shaped, catalase- and oxidase-negative bacterium, designated as Gsoil 193(T), was isolated from the soil of ginseng field in Pocheon province, South Korea. 16S rRNA gene sequence analysis showed that strain Gsoil 193(T) belonged to family Xanthomonadaceae and was most closely related to Lysobacter daecheongensis KCTC 12,600(T) (96.4 %), Lysobacter panaciterrae KCTC 12601(T) (96.3 %), Lysobacter dokdonensis DSM 17958(T) (96.3 %) and Lysobacter oligotrophicus JCM 18257(T) (95.6 %). Strain Gsoil 193(T) grew at temperatures between 20 and 30 °C with an optimum of 30 °C. The pH range for growth was 5-9 pH (optimum 6-7 pH). The predominant respiratory quinone was ubiquinone Q-8 and a fatty acid profile with iso-C15:0, iso-C16:0 and summed feature 9 (iso-C17:1 ω9c/C16:0 10-methyl) as the major fatty acids supported the affiliation of strain Gsoil 193(T) to the genus Lysobacter. The genomic DNA G+C content was 64.8 mol %. On the basis of the genotypic analysis, physiological and chemotaxonomic results indicate that strain Gsoil 193(T) represents a novel species of the genus Lysobacter, for which the name Lysobacter pocheonensis sp. nov. is proposed. The type strain is Gsoil 193(T) (= DSM 18338(T) = KCTC 12624(T)).
authors.affiliation: Department of Biotechnology, Hankyong National University, 327 Chungang-no, Anseong-si, Kyonggi-do, 456-749, Republic of Korea.|Center for Genetic Information, Graduate School of Bio and Information Technology, Hankyoung National University, 327 Chungang-no, Anseong-si, Kyonggi-do, 456-749, Republic of Korea.
authors.forename: Muhammad Zubair
authors.initials: MZ
authors.lastname: Siddiqi
authors.affiliation: Department of Biotechnology, Hankyong National University, 327 Chungang-no, Anseong-si, Kyonggi-do, 456-749, Republic of Korea. wandra@hknu.ac.kr.|Center for Genetic Information, Graduate School of Bio and Information Technology, Hankyoung National University, 327 Chungang-no, Anseong-si, Kyonggi-do, 456-749, Republic of Korea. wandra@hknu.ac.kr.
authors.forename: Wan-Taek
authors.initials: WT
authors.lastname: Im
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D053528:Lysobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D005894:Panax / Q000254:growth & development / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA gene; Lysobacter pocheonensis; Polyphasic taxonomy