title: Modelling of survival of patients with colon adenocarcinoma based on multivariable analysis of the state of cancer cell nuclear apparatus.
abstract: OBJECTIVE
  The creation of a mathematical model of survival in patients with colon adenocarcinoma based on multivariable analysis of the state of cancer cell nuclear apparatus.
  
  
  METHODS
  The study was performed on 141 samples of biopsy materials or material obtained during surgical treatment of the patients with colon adenocarcinoma or benign colon neoplasms with the use of histological, morphometric, densitometric, immunohistochemical and mathematical methods.
  
  
  RESULTS
  It has been shown that each discrete pattern of the state of adenocarcinoma cell nuclei (quantity of DNA, the number and volume of nuclear organizer regions, expression rates of Ki-67, Bcl-2 and p53) is prognostically invalid in the case of its separate use. Combination of these characteristics significantly enhances prognostic validity of the survival model. Based on equation of Cox proportional hazards, survival model of good quality for the patients with moderately and poorly differentiated adenocarcinoma and increased average DNA content in tumor cell nuclei has been created.
  
  
  CONCLUSIONS
  The proposed survival model for colon adenocarcinoma demonstrates the quality twice superior to the model based on the use of tumor grade only (G) which in fact is presently used as a sole common independent histological criterion of prognosis.
authors.affiliation: National Cancer Institute, Kyiv, 03022, Ukraine.
authors.forename: A N
authors.initials: AN
authors.lastname: Grabovoy
authors.affiliation: National Cancer Institute, Kyiv, 03022, Ukraine.
authors.forename: O O
authors.initials: OO
authors.lastname: Kolesnik
authors.affiliation: ESC "Institute of Biology" Taras Shevchenko National University, Kyiv 03022, Ukraine.
authors.forename: T M
authors.initials: TM
authors.lastname: Savchyn
authors.affiliation: National Cancer Institute, Kyiv, 03022, Ukraine.
authors.forename: S A
authors.initials: SA
authors.lastname: Antoniuk
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000453:epidemiology / Q000473:pathology; D002467:Cell Nucleus / Q000473:pathology*; D003106:Colon / Q000473:pathology*; D003110:Colonic Neoplasms / Q000175:diagnosis* / Q000453:epidemiology / Q000473:pathology; D004247:DNA / Q000032:analysis; D006801:Humans; D019394:Ki-67 Antigen / Q000032:analysis; D008954:Models, Biological; D011379:Prognosis; D016016:Proportional Hazards Models; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D012372:ROC Curve; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
chemical_list: D019394:Ki-67 Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D004247:DNA