title: SAMM50 Affects Mitochondrial Morphology through the Association of Drp1 in Mammalian Cells.
abstract: Mitochondrial fission and fusion activities are important for cell survival and function. Drp1 is a GTPase protein responsible for mitochondrial division, and SAMM50 is responsible for protein sorting and assembly. We demonstrated that SAMM50 overexpression results in Drp1-dependent mitochondrial fragmentation in HeLa cells. However, the mitochondrial fragmentation induced by SAMM50 overexpression could be reversed through co-expression with MFN2. Furthermore, SAMM50 interacts with Drp1 both in vivo and in vitro. The mitochondria in SAMM50 knockdown HeLa cells displayed a swollen phenotype, and the levels of the SAM complex and OPA1, along with the mitochondrial Drp1 levels, significantly decreased. In addition, mitochondrial inheritance was impaired in SAMM50 silenced cells. These results suggest that SAMM50 affects the Drp1-dependent mitochondrial morphology.
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, School of Pharmaceutical Sciences, Medical Research Institute, Wuhan University, Hubei, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Liu
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, School of Pharmaceutical Sciences, Medical Research Institute, Wuhan University, Hubei, China.|Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, School of Pharmaceutical Sciences, Medical Research Institute, Wuhan University, Hubei, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, School of Pharmaceutical Sciences, Medical Research Institute, Wuhan University, Hubei, China.
authors.forename: Han
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, School of Pharmaceutical Sciences, Medical Research Institute, Wuhan University, Hubei, China.|Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
authors.forename: Shiyi
authors.initials: S
authors.lastname: Pan
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, School of Pharmaceutical Sciences, Medical Research Institute, Wuhan University, Hubei, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wang
authors.affiliation: Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
authors.forename: Shiming
authors.initials: S
authors.lastname: Du
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, School of Pharmaceutical Sciences, Medical Research Institute, Wuhan University, Hubei, China.
authors.forename: Zixin
authors.initials: Z
authors.lastname: Deng
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, School of Pharmaceutical Sciences, Medical Research Institute, Wuhan University, Hubei, China.
authors.forename: Lianrong
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Wuhan University, Hubei, China.
authors.forename: Zhiyin
authors.initials: Z
authors.lastname: Song
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, School of Pharmaceutical Sciences, Medical Research Institute, Wuhan University, Hubei, China.
authors.forename: Shi
authors.initials: S
authors.lastname: Chen
mesh_terms: D034281:Dynamins; D020558:GTP Phosphohydrolases / Q000235:genetics / Q000378:metabolism*; D045325:HCT116 Cells; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D024101:Mitochondrial Proteins; C522815:SAMM50 protein, human; D020558:GTP Phosphohydrolases; C470018:MFN2 protein, human; C415198:OPA1 protein, human; C110768:DNM1L protein, human; D034281:Dynamins
keywords: Drp1; SAMM50; TALEN; fission; mitochondria; morphology