title: Determination of nine volatile N-nitrosamines in tobacco and smokeless tobacco products by dispersive solid-phase extraction with gas chromatography and tandem mass spectrometry.
abstract: A method was developed for the determination of nine volatile N-nitrosamines in tobacco and smokeless tobacco products. The targets are N-nitrosodimethylamine, N-nitrosopyrrolidine, N-nitrosopiperidine, N-nitrosomorpholine, N-nitrosoethylmethylamine, N-nitrosodiethylamine, N-nitrosodipropylamine, N-nitrosobuylmethylmine, and N-nitrosodibutylamine. The samples were treated by dispersive solid-phase extraction using 1 g of primary secondary amine and 0.5 g of carbon and then analyzed by gas chromatography with tandem mass spectrometry with an electron impact ion source. The recoveries for the targets ranged from 84 to 118%, with <16% relative standard deviations at three spiking levels of 0.5, 1.25, and 2.5 ng/g. The limits of detection ranged from 0.03 to 0.15 ng/g. With the use of the proposed method, we detected the presence of six nitrosamines in the range of 0.4-30.7 ng/g. The study demonstrated that the method could be used as a rapid, convenient, and high-throughput method for N-nitrosamines analysis in tobacco matrix.
authors.affiliation: Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Lv
authors.affiliation: Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China.
authors.forename: Junwei
authors.initials: J
authors.lastname: Guo
authors.affiliation: China Tobacco Zhejiang Industrial Co., Ltd, Hangzhou, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Yu
authors.affiliation: Gold leaf Manufacturing Center of China Tobacco Henan Industrial Co., Ltd, Zhengzhou, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Technique Center of Tobacco Production, PingDingshan Tobacco Company of Henan Tobacco Monopoly Bureau, PingDingshan, China.
authors.forename: Shimin
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China.
authors.forename: Huapeng
authors.initials: H
authors.lastname: Cui
authors.affiliation: Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China.
authors.forename: Xianjun
authors.initials: X
authors.lastname: Liu
authors.affiliation: Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China.
authors.forename: Leiyu
authors.initials: L
authors.lastname: Liu
authors.affiliation: Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China.
authors.forename: Shaofeng
authors.initials: S
authors.lastname: Liu
authors.affiliation: Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China.
authors.forename: Fuwei
authors.initials: F
authors.lastname: Xie
mesh_terms: D002849:Chromatography, Gas; D009602:Nitrosamines / Q000032:analysis*; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry; D062789:Tobacco Products / Q000032:analysis*; D014030:Tobacco, Smokeless / Q000032:analysis*; D055549:Volatile Organic Compounds / Q000032:analysis*
chemical_list: D009602:Nitrosamines; D055549:Volatile Organic Compounds
keywords: Dispersive solid-phase extraction; Gas chromatography with tandem mass spectrometry; N-nitrosamines; Tobacco