title: Titanium scaffold osteogenesis in healthy and osteoporotic rats is improved by the use of low-level laser therapy (GaAlAs).
abstract: The present study aimed to assess the effects of low-level laser therapy (GaAlAs) on the bone repair process within titanium scaffolds in the femurs of healthy and osteoporotic rats. Fifty-six rats were divided into four groups: group Sh: SHAM animals that received scaffolds; group LSh: SHAM animals that received scaffolds and were subjected to laser therapy; group OV: ovarietomized (OVX) animals that received scaffolds; and group LOV: OVX animals that received scaffolds and were subjected to laser therapy. Thirty days following ovariectomy or sham surgery, scaffolds were implanted in the left femurs of all animals in the study. Immediately after opening the surgical site, the inner part of the surgical cavity was stimulated with low-level laser (GaAlAs). In addition to this procedure, the laser group was also subjected to sessions of low-level laser therapy (LLLT) at 48-h intervals, with the first session performed immediately after surgery. The rats were sacrificed at 2 and 6 weeks, time in which femur fragments were submitted for histological and histomorphometric examination, and skin tissue above the scaffold was submitted to histological analysis. At the end of the study, greater bone formation was observed in the animals submitted to LLLT. At 2 and 6 weeks, statistically significant differences were observed between LSh and Sh groups (p = 0.009 and 0.0001) and LOV and OV (p = 0.0001 and 0.0001), respectively. No statistical difference was observed when assessing the estrogen variable. On the basis of our methodology and results, we conclude that LLLT improves and accelerates bone repair within titanium scaffolds in both ovariectomized and healthy rats, when compared to animals not subjected to radiation.
authors.affiliation: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, Univ Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo, 777, São José dos Campos, SP, Brazil, CEP 12245-000. luanamrv@gmail.com.
authors.forename: Luana Marotta Reis
authors.initials: LM
authors.lastname: de Vasconcellos
authors.affiliation: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, Univ Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo, 777, São José dos Campos, SP, Brazil, CEP 12245-000.
authors.forename: Mary Anne Moreira
authors.initials: MA
authors.lastname: Barbara
authors.affiliation: Department of Stomatology, Division of Periodontics, School of Dentistry, University of São Paulo, USP, Av. Lineu Prestes, 2227, São Paulo, SP, Brazil, CEP 05508-000.
authors.forename: Emanuel da Silva
authors.initials: ES
authors.lastname: Rovai
authors.affiliation: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, Univ Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo, 777, São José dos Campos, SP, Brazil, CEP 12245-000.
authors.forename: Mariana
authors.initials: M
authors.lastname: de Oliveira França
authors.affiliation: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, Univ Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo, 777, São José dos Campos, SP, Brazil, CEP 12245-000.
authors.forename: Zahra Fernandes
authors.initials: ZF
authors.lastname: Ebrahim
authors.affiliation: Department of Prosthodontics and Dental Materials, Institute of Science and Technology, Univ Estadual Paulista (UNESP), Av. Eng. Francisco José Longo, 777, São José dos Campos, SP, Brazil, CEP 12245-000.
authors.forename: Luis Gustavo Oliveira
authors.initials: LG
authors.lastname: de Vasconcellos
authors.affiliation: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, Univ Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo, 777, São José dos Campos, SP, Brazil, CEP 12245-000.
authors.forename: Camila Deco
authors.initials: CD
authors.lastname: Porto
authors.affiliation: Division of Materials, Air and Space Institute, CTA, Praça Mal. do Ar Eduardo Gomes 14, São José dos Campos, 12904-000, SP, Brazil.
authors.forename: Carlos Alberto Alves
authors.initials: CA
authors.lastname: Cairo
mesh_terms: D000818:Animals; D005260:Female; D005269:Femur; D028022:Low-Level Light Therapy / Q000379:methods*; D010012:Osteogenesis / Q000528:radiation effects*; D010052:Ovariectomy; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D054457:Tissue Scaffolds / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*; D014945:Wound Healing / Q000528:radiation effects*
chemical_list: D014025:Titanium
keywords: Dental implant; Laser therapy; Osseointegration; Ovariectomy; Titanium