title: Investigation of N-acetylcysteine on contralateral testis tissue injury by experimental testicular torsion: long-term effect.
abstract: BACKGROUND
  The aim of the study was to investigate long-term effects of N-acetylcysteine (NAC) on contralateral testes by experimental testicular torsion using histopathologic and biochemical parameters.
  
  
  METHODS
  Eighteen rats were randomized and divided into 3 groups. In group 1, the control group (C), laparotomy was performed and the left and right testes were excised 2 months later. In group 2, the torsion and detorsion group (T), the torsion was performed by rotating the left testis 720° to clockwise direction, and then 4 hours later, detorsion was performed; 2 months later, contralateral testes were removed. In group 3, the NAC adding torsion and detorsion group (T+NAC), the torsion was performed by rotating the left testis 720° to clockwise direction, and then 4 hours later, detorsion was performed. N-acetylcysteine was given intraperitoneally 30 minutes before detorsion and following 5 days after detorsion.
  
  
  RESULTS
  GPx activities were increased in the T and T+NAC groups compared with the control (P = .008 and P = .016, respectively). Seminiferous tubule diameter thickness is decreased in the torsion group compared with the control group and decreased in the T+NAC group compared with the torsion group (P < .05).
  
  
  CONCLUSIONS
  In the long term as implied from the histopathologic findings, NAC has beneficial effects against contralateral testis tissue injury induced by testicular torsion.
authors.affiliation: Department of Medical Biochemistry, Karadeniz Technical University, Faculty of Medicine, Trabzon, Turkey. Electronic address: akinbodur28@gmail.com.
authors.forename: Akın
authors.initials: A
authors.lastname: Bodur
authors.affiliation: Department of Medical Biochemistry, Karadeniz Technical University, Faculty of Medicine, Trabzon, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Alver
authors.affiliation: Department of Medical Biochemistry, Karadeniz Technical University, Faculty of Medicine, Trabzon, Turkey.
authors.forename: Cemil
authors.initials: C
authors.lastname: Kahraman
authors.affiliation: Department of Medical Biochemistry, Karadeniz Technical University, Faculty of Medicine, Trabzon, Turkey.
authors.forename: Diler Us
authors.initials: DU
authors.lastname: Altay
authors.affiliation: Department of Medical Biochemistry, Karadeniz Technical University, Faculty of Medicine, Trabzon, Turkey.
authors.forename: İmran
authors.initials: İ
authors.lastname: İnce
mesh_terms: D000111:Acetylcysteine / Q000627:therapeutic use*; D000818:Animals; D002374:Catalase / Q000378:metabolism; D004195:Disease Models, Animal; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D013086:Spermatic Cord Torsion / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism; D013733:Testicular Diseases / Q000209:etiology / Q000517:prevention & control*; D013997:Time Factors
chemical_list: D016166:Free Radical Scavengers; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D000111:Acetylcysteine