title: Identifying NK Alloreactive Donors for Haploidentical Hematopoietic Stem Cell Transplantation.
abstract: NK cell alloreactivity mediated by donor NK cells is a fundamental therapeutic tool in HLA haplotype mismatched hematopoietic transplantation in adult acute myeloid leukemia and pediatric acute lymphoblastic leukemias. NK cell is mediated by donor NK cells recovering very early after transplant. The self donor KIR ligands educates the donor NK repertoire and arms functional NK cells which express inhibitory killer cell immunoglobulin-like receptor(s) (KIRs) for self-class I ligand(s), They sense missing expression of donor KIR ligand(s) in the recipient and mediate alloreactivity. Donor-versus-recipient NK cell alloreactivity is evaluated by KIR genotyping and phenotyping and functional assay.
authors.affiliation: Division of Hematology and Clinical Immunology, Department of Medicine, University of Perugia, Perugia, Italy. loredana.ruggeri@unipg.it.
authors.forename: Loredana
authors.initials: L
authors.lastname: Ruggeri
authors.affiliation: Division of Hematology and Clinical Immunology, Department of Medicine, University of Perugia, Perugia, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Mancusi
authors.affiliation: Division of Hematology and Clinical Immunology, Department of Medicine, University of Perugia, Perugia, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Urbani
authors.affiliation: Division of Hematology and Clinical Immunology, Department of Medicine, University of Perugia, Perugia, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Velardi
mesh_terms: D060005:Genotyping Techniques; D018380:Hematopoietic Stem Cell Transplantation; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D054341:Receptors, KIR3DL1 / Q000235:genetics; D014019:Tissue Donors
chemical_list: C515083:KIR3DL1 protein, human; D054341:Receptors, KIR3DL1
keywords: HLA haplotype mismatched hematopoietic transplantation; KIR genotyping; KIR phenotyping; NK cell alloreactivity; NK cells