title: Methods for the Identification of PTEN-Targeting MicroRNAs.
abstract: The identification of PTEN-targeting microRNAs usually starts from an in silico bioinformatic prediction and then requires a careful experimental validation that exploits both heterologous and endogenous systems. Here we describe the methods used to carry on these analyses and experiments, examining pitfalls and alternatives for each step. Moreover, we give an overview of the latest high-throughput microRNA target identification techniques which offer a more comprehensive view of the microRNAs that can bind a fundamental tumor suppressor such as PTEN.
authors.affiliation: Oncogenomics Unit, Istituto Toscano Tumori, Pisa, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Tuccoli
authors.affiliation: Oncogenomics Unit, Istituto Toscano Tumori, Pisa, Italy.|University of Siena, Siena, Italy.
authors.forename: Marianna
authors.initials: M
authors.lastname: Vitiello
authors.affiliation: Oncogenomics Unit, Istituto Toscano Tumori, Pisa, Italy.|University of Siena, Siena, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Marranci
authors.affiliation: LISM, IIT-IFC, CNR, Pisa, Italy.|Department of Computer Science, University of Pisa, Pisa, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Russo
authors.affiliation: Oncogenomics Unit, Istituto Toscano Tumori, Pisa, Italy. laura.poliseno@gmail.com.|CNR-IFC, Pisa, Italy. laura.poliseno@gmail.com.
authors.forename: Laura
authors.initials: L
authors.lastname: Poliseno
mesh_terms: D020413:3' Untranslated Regions; D000465:Algorithms; D003198:Computer Simulation; D005786:Gene Expression Regulation; D005821:Genetic Techniques; D045325:HCT116 Cells; D006801:Humans; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D016296:Mutagenesis; D051059:PTEN Phosphohydrolase / Q000032:analysis / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human
keywords: 3′ Untranslated region; MicroRNA responsive element; MicroRNAs; PTEN; Posttranscriptional regulation