title: High-Resolution Microfluidic Single-Cell Transcriptional Profiling Reveals Clinically Relevant Subtypes among Human Stem Cell Populations Commonly Utilized in Cell-Based Therapies.
abstract: Stem cell therapies can promote neural repair and regeneration, yet controversy regarding optimal cell source and mechanism of action has slowed clinical translation, potentially due to undefined cellular heterogeneity. Single-cell resolution is needed to identify clinically relevant subpopulations with the highest therapeutic relevance. We combine single-cell microfluidic analysis with advanced computational modeling to study for the first time two common sources for cell-based therapies, human NSCs and MSCs. This methodology has the potential to logically inform cell source decisions for any clinical application.
authors.affiliation: Department of Surgery, Stanford University School of Medicine , Stanford, CA , USA.
authors.forename: Robert C
authors.initials: RC
authors.lastname: Rennert
authors.affiliation: Department of Neurosurgery, Stanford University School of Medicine , Stanford, CA , USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Schäfer
authors.affiliation: Department of Neurosurgery, Stanford University School of Medicine , Stanford, CA , USA.
authors.forename: Tonya
authors.initials: T
authors.lastname: Bliss
authors.affiliation: Department of Surgery, Stanford University School of Medicine , Stanford, CA , USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Januszyk
authors.affiliation: Department of Surgery, Stanford University School of Medicine , Stanford, CA , USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Sorkin
authors.affiliation: Department of Neurosurgery, Stanford University School of Medicine , Stanford, CA , USA.
authors.forename: Achal S
authors.initials: AS
authors.lastname: Achrol
authors.affiliation: Department of Surgery, Stanford University School of Medicine , Stanford, CA , USA.
authors.forename: Melanie
authors.initials: M
authors.lastname: Rodrigues
authors.affiliation: Department of Surgery, Stanford University School of Medicine , Stanford, CA , USA.
authors.forename: Zeshaan N
authors.initials: ZN
authors.lastname: Maan
authors.affiliation: Department of Orthopedics, University Hospital Tübingen , Tübingen , Germany.
authors.forename: Torsten
authors.initials: T
authors.lastname: Kluba
authors.affiliation: Department of Neurosurgery, Stanford University School of Medicine , Stanford, CA , USA.
authors.forename: Gary K
authors.initials: GK
authors.lastname: Steinberg
authors.affiliation: Department of Surgery, Stanford University School of Medicine , Stanford, CA , USA.
authors.forename: Geoffrey C
authors.initials: GC
authors.lastname: Gurtner
keywords: cellular heterogeneity; single-cell analysis; stem cell therapeutics