title: The Role of Heat Shock Protein 90B1 in Patients with Polycystic Ovary Syndrome.
abstract: Polycystic ovary syndrome (PCOS) is a heterogenetic disorder in women that is characterized by arrested follicular growth and anovulatory infertility. The altered protein expression levels in the ovarian tissues reflect the molecular defects in folliculogenesis. To identify aberrant protein expression in PCOS, we analyzed protein expression profiles in the ovarian tissues of patients with PCOS. We identified a total of 18 protein spots that were differentially expressed in PCOS compared with healthy ovarian samples. A total of 13 proteins were upregulated and 5 proteins were downregulated. The expression levels of heat shock protein 90B1 (HSP90B1) and calcium signaling activator calmodulin 1 (CALM1) were increased by at least two-fold. The expression levels of HSP90B1 and CALM1 were positively associated with ovarian cell survival and negatively associated with caspase-3 activation and apoptosis. Knock-down of HSP90B1 with siRNA attenuated ovarian cell survival and increased apoptosis. In contrast, ovarian cell survival was improved and cell apoptosis was decreased in cells over-expressing HSP90B1. These results demonstrated the pivotal role of HSP90B1 in the proliferation and survival of ovarian cells, suggesting a critical role for HSP90B1 in the pathogenesis of PCOS. We also observed a downregulation of anti-inflammatory activity-related annexin A6 (ANXA6) and tropomyosin 2 (TPM2) compared with the normal controls, which could affect cell division and folliculogenesis in PCOS. This is the first study to identify novel altered gene expression in the ovarian tissues of patients with PCOS. These findings may have significant implications for future diagnostic and treatment strategies for PCOS using molecular interventions.
authors.affiliation: Department of Obstetrics and Gynecology, Guangdong Women and Children Hospital, Guangzhou, Guangdong, China.|Laboratory of Chinese Medicine Quality Research, Macau University of Science and Technology, Macau, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Laboratory of Chinese Medicine Quality Research, Macau University of Science and Technology, Macau, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Mo
authors.affiliation: Guangzhou Family Planning Specialty Hospital, Guangzhou, Guangdong, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, Guangdong Women and Children Hospital, Guangzhou, Guangdong, China.
authors.forename: Yongxian
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Obstetrics and Gynecology, Guangdong Women and Children Hospital, Guangzhou, Guangdong, China.
authors.forename: Xiuhong
authors.initials: X
authors.lastname: Peng
authors.affiliation: Department of Obstetrics and Gynecology, Guangdong Women and Children Hospital, Guangzhou, Guangdong, China.
authors.forename: Xiping
authors.initials: X
authors.lastname: Luo
mesh_terms: D000328:Adult; D017209:Apoptosis; D002147:Calmodulin / Q000096:biosynthesis; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D004789:Enzyme Activation; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D010053:Ovary / Q000378:metabolism* / Q000473:pathology; D011085:Polycystic Ovary Syndrome / Q000378:metabolism* / Q000473:pathology; D014335:Tropomyosin / Q000096:biosynthesis
chemical_list: C000607143:CALM1 protein, human; D002147:Calmodulin; D008562:Membrane Glycoproteins; D014335:Tropomyosin; C053387:endoplasmin; C505407:CASP3 protein, human; D053148:Caspase 3