title: Isolation and Characterization of Cancer Stem Cells of the Non-Small-Cell Lung Cancer (A549) Cell Line.
abstract: Cancer is a major health problem worldwide. The failure of current treatments to completely eradicate cancer cells often leads to cancer recurrence and dissemination. Studies have suggested that tumor growth and spread are driven by a minority of cancer cells that exhibit characteristics similar to those of normal stem cells, thus these cells are called cancer stem cells (CSCs). CSCs are believed to play an important role in initiating and promoting cancer. CSCs are resistant to currently available cancer therapies, and understanding the mechanisms that control the growth of CSCs might have great implications for cancer therapy. Cancer cells are consist of heterogeneous population of cells, thus methods of identification, isolation, and characterisation of CSCs are fundamental to obtain a pure CSC populations. Therefore, this chapter describes in detail a method for isolating and characterizing a pure population of CSCs from heterogeneous population of cancer cells and CSCs based on specific cell surface markers.
authors.affiliation: Regenerative Medicine Cluster, Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia, Penang, 13200, Malaysia.
authors.forename: Noor Hanis Abu
authors.initials: NHA
authors.lastname: Halim
authors.affiliation: Regenerative Medicine Cluster, Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia, Penang, 13200, Malaysia.
authors.forename: Norashikin
authors.initials: N
authors.lastname: Zakaria
authors.affiliation: Regenerative Medicine Cluster, Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia, Penang, 13200, Malaysia.
authors.forename: Nazilah Abdul
authors.initials: NA
authors.lastname: Satar
authors.affiliation: Regenerative Medicine Cluster, Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia, Penang, 13200, Malaysia. badrul@usm.my.
authors.forename: Badrul Hisham
authors.initials: BH
authors.lastname: Yahaya
mesh_terms: D000072283:A549 Cells; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000473:pathology*; D018929:Cell Culture Techniques / Q000379:methods*; D019070:Cell Lineage; D002469:Cell Separation / Q000379:methods*; D006801:Humans; D014411:Neoplastic Stem Cells / Q000473:pathology*
keywords: Cancer stem cells (CSCs); Characterization; Method; Sorting