title: One step antibody-mediated isolation and patterning of multiple cell types in microfluidic devices.
abstract: Cell-cell interactions play a key role in regeneration, differentiation, and basic tissue function taking place under physiological shear forces. However, current solutions to mimic such interactions by micro-patterning cells within microfluidic devices have low resolution, high fabrication complexity, and are limited to one or two cell types. Here, we present a microfluidic platform capable of laminar patterning of any biotin-labeled peptide using streptavidin-based surface chemistry. The design permits the generation of arbitrary cell patterns from heterogeneous mixtures in microfluidic devices. We demonstrate the robust co-patterning of α-CD24, α-ASGPR-1, and α-Tie2 antibodies for rapid isolation and co-patterning of mixtures of hepatocytes and endothelial cells. In addition to one-step isolation and patterning, our design permits step-wise patterning of multiple cell types and empty spaces to create complex cellular geometries in vitro. In conclusion, we developed a microfluidic device that permits the generation of perfusable tissue-like patterns in microfluidic devices by directly injecting complex cell mixtures such as differentiated stem cells or tissue digests with minimal sample preparation.
authors.affiliation: Alexander Grass Center for Bioengineering, The Selim and Rachel Benin School for Computer Science and Engineering, The Hebrew University of Jerusalem , Jerusalem 9190401, Israel.
authors.forename: Danny
authors.initials: D
authors.lastname: Bavli
authors.affiliation: Alexander Grass Center for Bioengineering, The Selim and Rachel Benin School for Computer Science and Engineering, The Hebrew University of Jerusalem , Jerusalem 9190401, Israel.
authors.forename: Elishai
authors.initials: E
authors.lastname: Ezra
authors.affiliation: Department of Cell and Developmental Biology, Silberman Institute of Life Sciences, The Hebrew University of Jerusalem , Jerusalem 9190401, Israel.
authors.forename: Daniel
authors.initials: D
authors.lastname: Kitsberg
authors.forename: Margarita
authors.identifier: 0000-0001-9678-7829
authors.initials: M
authors.lastname: Vosk-Artzi
authors.affiliation: Department of Chemical Engineering and Barnett Institute of Chemical and Biological Analysis, Northeastern University , Boston, Massachusetts 02115, USA.
authors.forename: Shashi K
authors.initials: SK
authors.lastname: Murthy
authors.forename: Yaakov
authors.initials: Y
authors.lastname: Nahmias