title: Renal tubular injury induced by ischemia promotes the formation of calcium oxalate crystals in rats with hyperoxaluria.
abstract: Hyperoxaluria and cell injury are key factors in urolithiasis. Oxalate metabolism may be altered by renal dysfunction and therefore, impact the deposition of calcium oxalate (CaOx) crystals. We investigated the relationship of renal function, oxalate metabolism and CaOx crystal deposition in renal ischemia. One hundred male Sprague-Dawley rats were randomly divided into four groups. Hyperoxaluria model (Group A and B) was established by feeding rats with 0.75 % ethylene glycol (EG). The left renal pedicle was clamped for 30 min to establish renal ischemia Groups (B and C), while Groups A and D underwent sham operation. Then, serum and urine oxalate (Ox), creatinine (Cr) and urea nitrogen (UN) levels were evaluated by liquid chromatography mass spectrometry (LCMS) and ion mass spectrum (IMS) at days 0, 2, 4, 7, and 14. CaOx crystallization was assessed by transmission electron microscope (TEM). A temporal and significant increase of serum Cr and UN levels was observed in Groups B and C compared to values obtained for Groups A and D (P < 0.05). Ox levels in serum and urine were significantly higher in Groups A and B than in the other two groups from day 7 (P < 0.05). In addition, CaOx crystallization was observed in both Groups A and B, but Group B showed earlier and more pronounced crystal deposition in the renal tissue. Our results indicated that renal tubular injury induced by renal ischemia might not affect Ox levels but could promote CaOx crystal retention under hyperoxaluria.
authors.affiliation: Department of Urology, The Affiliated Hospital of Qingdao University, Haier Road No. 59, Qingdao, 266101, Shandong Province, People's Republic of China.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Urology, The First Affiliated Hospital of Harbin Medical University, Youzheng Street No. 23, Harbin, 150001, Heilongjiang, People's Republic of China.
authors.forename: Wanpeng
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Urology, Affiliated Hospital of Hebei University of Engineering, Congtai Road No. 81, Handan, 056002, Hebei Province, People's Republic of China.
authors.forename: Limei
authors.initials: L
authors.lastname: Hui
authors.affiliation: Department of Urology, Affiliated Hospital of Hebei University of Engineering, Congtai Road No. 81, Handan, 056002, Hebei Province, People's Republic of China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Urology, The Affiliated Hospital of Qingdao University, Haier Road No. 59, Qingdao, 266101, Shandong Province, People's Republic of China.
authors.forename: Xuecheng
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Urology, The Affiliated Hospital of Qingdao University, Haier Road No. 59, Qingdao, 266101, Shandong Province, People's Republic of China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Urology, The Affiliated Hospital of Qingdao University, Haier Road No. 59, Qingdao, 266101, Shandong Province, People's Republic of China. caoynw@163.com.
authors.forename: Haitao
authors.initials: H
authors.lastname: Niu
mesh_terms: D000818:Animals; D002129:Calcium Oxalate / Q000378:metabolism*; D003460:Crystallization; D006959:Hyperoxaluria / Q000150:complications*; D007511:Ischemia / Q000150:complications*; D007668:Kidney / Q000098:blood supply*; D007684:Kidney Tubules / Q000473:pathology*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D052878:Urolithiasis / Q000209:etiology*
chemical_list: D002129:Calcium Oxalate
keywords: Calcium oxalate; Ischemia; Renal dysfunction; Urolithiasis