title: Cerium Oxide-Incorporated Calcium Silicate Coating Protects MC3T3-E1 Osteoblastic Cells from H2O2-Induced Oxidative Stress.
abstract: Oxidative stress regulates cellular functions in multiple pathological conditions, including bone formation by osteoblastic cells. In this work, the protective effects of cerium oxide (CeO2)-incorporated calcium silicate (CeO2-CS) coating on the response of osteoblasts to H2O2-induced oxidative stress and the related mechanism were examined. CeO2incorporation significantly improved osteoblast viability and reduced cell apoptosis caused by H2O2when compared with the control. H2O2-induced reduction of differentiation marker alkaline phosphatase (ALP) was recovered in the presence of the CeO2-CS coating. The above effects were mediated by the antioxidant effect of CeO2. The CeO2-CS coating immersed in 0.1 mM H2O2aqueous solution was able to degrade 64 % of it in 1 week. In addition, CeO2incorporation decreased reactive oxygen species (ROS) production and suppressed malondialdehyde (MDA) formation in H2O2-treated osteoblasts. Taken together, CeO2-CS biomedical coatings with antioxidant property would be promising for bone regeneration under oxidative stress.
authors.affiliation: Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.affiliation: Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, China.
authors.forename: Youtao
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, China.
authors.forename: Mingyu
authors.initials: M
authors.lastname: You
authors.affiliation: Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Huang
authors.affiliation: Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, China. xbzheng@mail.sic.ac.cn.
authors.forename: Xuebin
authors.initials: X
authors.lastname: Zheng
mesh_terms: D000818:Animals; D017610:Calcium Compounds / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D002563:Cerium / Q000737:chemistry / Q000494:pharmacology*; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D017640:Silicates / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D017610:Calcium Compounds; D020099:Coated Materials, Biocompatible; D017640:Silicates; D002563:Cerium; C030583:ceric oxide; D006861:Hydrogen Peroxide; C031293:calcium silicate
keywords: Antioxidant; Calcium silicate; CeO2; Osteoblast; Oxidative stress