title: Synthesis of polymers and nanoparticles bearing polystyrene sulfonate brushes for chemokine binding.
abstract: The movement of leukocytes to the site of inflammation in response to injury or infection is orchestrated by chemokines binding and signaling through cognate receptors. The interaction between sulfated tyrosine residues on the flexible N-terminal tail of the receptor with positively charged regions of the chemokine is one of the key recognition features that facilitates binding. In this manuscript we describe the synthesis of polymers and silica nanoparticles bearing polystyrene sulfonate brushes to mimic the sulfated tyrosine residues. We show that both the polymers and nanoparticles possess high binding affinity for the chemokine monocyte chemoattractant protein-1 (MCP-1) in monomeric and dimeric form. We also demonstrate key differences in the relative affinity for the chemokine for the free polymer versus the polymer-derived nanoparticle system.
authors.affiliation: School of Chemistry, The University of Sydney, New South Wales 2006, Australia. richard.payne@sydney.edu.au.
authors.forename: Naatasha
authors.initials: N
authors.lastname: Isahak
authors.affiliation: Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Victoria, Australia.
authors.forename: Julie
authors.initials: J
authors.lastname: Sanchez
authors.affiliation: School of Chemistry, The University of Sydney, New South Wales 2006, Australia. richard.payne@sydney.edu.au and Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK and Faculty of Pharmacy and Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, VIC 3052, Australia.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Perrier
authors.affiliation: Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Victoria, Australia.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Stone
authors.affiliation: School of Chemistry, The University of Sydney, New South Wales 2006, Australia. richard.payne@sydney.edu.au.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Payne
mesh_terms: D001665:Binding Sites; D018932:Chemokine CCL2 / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D011137:Polystyrenes / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2; D011137:Polystyrenes; C003321:polystyrene sulfonic acid