title: Wound healing modulation by a latex protein-containing polyvinyl alcohol biomembrane.
abstract: In a previous study, we performed the chemical characterization of a polyvinyl alcohol (PVA) membrane supplemented with latex proteins (LP) displaying wound healing activity, and its efficacy as a delivery system was demonstrated. Here, we report on aspects of the mechanism underlying the performance of the PVA-latex protein biomembrane on wound healing. LP-PVA, but not PVA, induced more intense leukocyte (neutrophil) migration and mast cell degranulation during the inflammatory phase of the cicatricial process. Likewise, LP-PVA induced an increase in key markers and mediators of the inflammatory response (myeloperoxidase activity, nitric oxide, TNF, and IL-1β). These results demonstrated that LP-PVA significantly accelerates the early phase of the inflammatory process by upregulating cytokine release. This remarkable effect improves the subsequent phases of the healing process. The polyvinyl alcohol membrane was fully absorbed as an inert support while LP was shown to be active. It is therefore concluded that the LP-PVA is a suitable bioresource for biomedical engineering.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular/UFC Campus do Pici 6033, Fortaleza, Ceará, Brazil. r_marcio@hotmail.com.
authors.forename: Márcio V
authors.initials: MV
authors.lastname: Ramos
authors.affiliation: Departamento de Fisiologia e Farmacologia/UFC, Fortaleza, Ceará, Brazil.
authors.forename: Nylane Maria N
authors.initials: NM
authors.lastname: de Alencar
authors.affiliation: Centro Universitário Estácio do Ceará, Via Corpvs, Fortaleza, Ceará, Brazil.
authors.forename: Raquel S B
authors.initials: RS
authors.lastname: de Oliveira
authors.affiliation: Departamento de Fisiologia e Farmacologia/UFC, Fortaleza, Ceará, Brazil.
authors.forename: Lyara B N
authors.initials: LB
authors.lastname: Freitas
authors.affiliation: Departamento de Fisiologia e Farmacologia/UFC, Fortaleza, Ceará, Brazil.
authors.forename: Karoline S
authors.initials: KS
authors.lastname: Aragão
authors.affiliation: Divisão de Dermatologia, Faculdade de Medicina de Ribeirão Preto, São Paulo, Brazil.
authors.forename: Thiago Antônio M
authors.initials: TA
authors.lastname: de Andrade
authors.affiliation: Divisão de Dermatologia, Faculdade de Medicina de Ribeirão Preto, São Paulo, Brazil.
authors.forename: Marco Andrey C
authors.initials: MA
authors.lastname: Frade
authors.affiliation: Departamento de Morfologia/UFC, Fortaleza, Ceará, Brazil.
authors.forename: Gerly Anne C
authors.initials: GA
authors.lastname: Brito
authors.affiliation: Centro Universitário Estácio do Ceará, Via Corpvs, Fortaleza, Ceará, Brazil.
authors.forename: Ingrid Samantha T
authors.initials: IS
authors.lastname: de Figueiredo
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D031107:Calotropis / Q000737:chemistry; D015550:Cell Degranulation / Q000187:drug effects; D004195:Disease Models, Animal; D004337:Drug Carriers; D004339:Drug Compounding; D018836:Inflammation Mediators / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D007840:Latex / Q000302:isolation & purification / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008567:Membranes, Artificial; D051379:Mice; D020556:Neutrophil Infiltration / Q000187:drug effects; D009569:Nitric Oxide / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D008517:Phytotherapy; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D012867:Skin / Q000187:drug effects* / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*; D014950:Wounds, Penetrating / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
chemical_list: D004337:Drug Carriers; C587672:IL1B protein, mouse; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D007840:Latex; D008567:Membranes, Artificial; D010940:Plant Proteins; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D011142:Polyvinyl Alcohol; D009195:Peroxidase
keywords: Biomembrane; Calotropis procera; Polyvinyl alcohol; Wound