title: Transcriptome analysis reveals physiological characteristics required for magnetosome formation in Magnetospirillum gryphiswaldense MSR-1.
abstract: Magnetosome synthesis ability of Magnetospirillum gryphiswaldense MSR-1 in an autofermentor can be precisely controlled through strict control of dissolved oxygen concentration. In this study, using transcriptome data we discovered gene transcriptional differences and compared physiological characteristics of MSR-1 cells cultured under aerobic (high-oxygen) and micro-aerobic (low-oxygen) conditions. The results showed that 77 genes were up-regulated and 95 genes were down-regulated significantly under micro-aerobic situation. These genes were involved primarily in the categories of cell metabolism, transport, regulation and unknown-function proteins. The nutrient transport and physiological metabolism were slowed down under micro-aerobic condition, whereas dissimilatory denitrification pathways were activated and it may supplemental energy was made available for magnetosome synthesis. The result suggested that the genes of magnetosome membrane proteins (Mam and Mms) are not directly regulated by oxygen level, or are constitutively expressed. A proposed regulatory network of differentially expressed genes reflects the complexity of physiological metabolism in MSR-1, and suggests that some yet-unknown functional proteins play important roles such as ferric iron uptake and transport during magnetosome synthesis. The transcriptome data provides a holistic view of the responses of MSR-1 cells to differing oxygen levels. This approach will give new insights into general principles of magnetosome formation.
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, P. R. China.|France-China Bio-mineralization and Nano-structure Laboratory, Beijing, 100193, P. R. China.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, P. R. China.|France-China Bio-mineralization and Nano-structure Laboratory, Beijing, 100193, P. R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, P. R. China.|France-China Bio-mineralization and Nano-structure Laboratory, Beijing, 100193, P. R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Tianjin Biochip Corporation, Tianjin, 300457, P. R. China.
authors.forename: Yinjia
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, P. R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, P. R. China.|France-China Bio-mineralization and Nano-structure Laboratory, Beijing, 100193, P. R. China.
authors.forename: Weijia
authors.initials: W
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, P. R. China.|France-China Bio-mineralization and Nano-structure Laboratory, Beijing, 100193, P. R. China.
authors.forename: Tong
authors.initials: T
authors.lastname: Wen
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, P. R. China.|France-China Bio-mineralization and Nano-structure Laboratory, Beijing, 100193, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, P. R. China.|France-China Bio-mineralization and Nano-structure Laboratory, Beijing, 100193, P. R. China.
authors.forename: Meiqing
authors.initials: M
authors.lastname: Zuo
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, P. R. China.
authors.forename: Ziding
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, P. R. China.|France-China Bio-mineralization and Nano-structure Laboratory, Beijing, 100193, P. R. China.
authors.forename: Jiesheng
authors.initials: J
authors.lastname: Tian
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, P. R. China.|France-China Bio-mineralization and Nano-structure Laboratory, Beijing, 100193, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, P. R. China.|France-China Bio-mineralization and Nano-structure Laboratory, Beijing, 100193, P. R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Tianjin Biochip Corporation, Tianjin, 300457, P. R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, P. R. China.|France-China Bio-mineralization and Nano-structure Laboratory, Beijing, 100193, P. R. China.
authors.forename: Jilun
authors.initials: J
authors.lastname: Li
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D053263:Gene Regulatory Networks; D055944:Magnetosomes / Q000378:metabolism*; D042201:Magnetospirillum / Q000235:genetics* / Q000378:metabolism*; D053858:Metabolic Networks and Pathways / Q000235:genetics