title: Low-Cost Nanoribbon Sensors for Protein Analysis in Human Serum Using a Miniature Bead-Based Enzyme-Linked Immunosorbent Assay.
abstract: We describe a low cost thin-film transistor (TFT) nanoribbon sensor for detection of the inflammatory biomarker C-reactive protein (CRP) in human serum via a miniature bead-based enzyme-linked immunosorbent assay (ELISA). The TFT nanoribbon sensor measures the reaction products from the ELISA via pH changes. The bead-based ELISA decouples the protein functionalization steps from the sensor surface, increasing the signal and simplifying the assay. The ability to directly sense proteins in human serum in this way overcomes the Debye length limitation associated with nanowire and nanoribbon biosensors. Compared to classically fabricated nanowires, the TFT nanoribbon sensors are simple, extremely easy to fabricate, and should therefore be much cheaper to manufacture. TFT nanoribbon sensors, configured to measure pH, were used for quantitative detection of CRP spiked into human serum at concentrations as low as 0.2 ng/mL, which is 10 000 times lower than needed for diagnostic purposes, providing the potential for applications that require very high sensitivity.
authors.affiliation: Department of Electronics and Computer Science, and Institute for Life Sciences, University of Southampton , Southampton SO17 1BJ, United Kingdom.
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Hu
authors.affiliation: Department of Electronics and Computer Science, and Institute for Life Sciences, University of Southampton , Southampton SO17 1BJ, United Kingdom.
authors.forename: Ioannis
authors.initials: I
authors.lastname: Zeimpekis
authors.affiliation: Department of Electronics and Computer Science, and Institute for Life Sciences, University of Southampton , Southampton SO17 1BJ, United Kingdom.
authors.forename: Kai
authors.initials: K
authors.lastname: Sun
authors.affiliation: Sharp Laboratories of Europe , Oxford OX4 4GB, United Kingdom.
authors.forename: Sally
authors.initials: S
authors.lastname: Anderson
authors.affiliation: Department of Electronics and Computer Science, and Institute for Life Sciences, University of Southampton , Southampton SO17 1BJ, United Kingdom.
authors.forename: Peter
authors.initials: P
authors.lastname: Ashburn
authors.affiliation: Department of Electronics and Computer Science, and Institute for Life Sciences, University of Southampton , Southampton SO17 1BJ, United Kingdom.
authors.forename: Hywel
authors.initials: H
authors.lastname: Morgan
mesh_terms: D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation; D002097:C-Reactive Protein / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000191:economics / Q000295:instrumentation; D006801:Humans; D037742:Nanotubes, Carbon / Q000737:chemistry / Q000191:economics*
chemical_list: D037742:Nanotubes, Carbon; D002097:C-Reactive Protein