title: Lyotropic Liquid Crystalline Cubic Phases as Versatile Host Matrices for Membrane-Bound Enzymes.
abstract: Lyotropic liquid crystalline cubic mesophases can function as host matrices for enzymes because of their biomimetic structural characteristics, optical transparency, and capability to coexist with water. This study demonstrates that the in meso immobilized membrane-bound enzyme d-fructose dehydrogenase (FDH) preserves its full activity, follows ideal Michaelis-Menten kinetics, and shows improved stability compared to its behavior in solution. Even after 5 days, the immobilized FDH retained its full activity in meso, whereas a model hydrophilic enzyme, horseradish peroxidase, maintained only 21% of its original activity. We reason that the lipidic bilayers in the three-dimensional structures of cubic mesophases provide an ideal environment for the reconstitution of a membrane-bound enzyme. The preserved activity, long-term stability, and reusability demonstrate that these hybrid nanomaterials are ideal matrices for biosensing and biocatalytic fuel cell applications.
authors.affiliation: Food and Soft Materials Science, Department of Health Science and Technology, ETH Zurich , Schmelzbergstrasse 9, CH-8092 Zurich, Switzerland.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Sun
authors.affiliation: Food and Soft Materials Science, Department of Health Science and Technology, ETH Zurich , Schmelzbergstrasse 9, CH-8092 Zurich, Switzerland.
authors.forename: Jijo J
authors.initials: JJ
authors.lastname: Vallooran
authors.affiliation: Food and Soft Materials Science, Department of Health Science and Technology, ETH Zurich , Schmelzbergstrasse 9, CH-8092 Zurich, Switzerland.|Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University , 381 Royal Parade, Parkville, Victoria 3052, Australia.
authors.forename: Wye-Khay
authors.initials: WK
authors.lastname: Fong
authors.affiliation: Food and Soft Materials Science, Department of Health Science and Technology, ETH Zurich , Schmelzbergstrasse 9, CH-8092 Zurich, Switzerland.
authors.forename: Raffaele
authors.initials: R
authors.lastname: Mezzenga
mesh_terms: D002237:Carbohydrate Dehydrogenases / Q000737:chemistry / Q000378:metabolism; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D050866:Liquid Crystals / Q000737:chemistry*; D008567:Membranes, Artificial
chemical_list: D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D002237:Carbohydrate Dehydrogenases; C028761:D-fructose 5-dehydrogenase; D006735:Horseradish Peroxidase