title: POLYPHASIC CHARACTERIZATION OF THREE STRAINS OF ANABAENA RENIFORMIS AND APHANIZOMENON APHANIZOMENOIDES (CYANOBACTERIA) AND THEIR RECLASSIFICATION TO SPHAEROSPERMUM GEN. NOV. (INCL. ANABAENA KISSELEVIANA)(1).
abstract: Occurrences of rare cyanobacteria Anabaena reniformis Lemmerm. and Aphanizomenon aphanizomenoides (Forti) Horecká et Komárek were recently detected at several localities in the Czech Republic. Two monoclonal strains of An. reniformis and one strain of Aph. aphanizomenoides were isolated from distant localities and different sampling years. They were characterized by a combination of morphological, genetic, and biochemical approaches. For the first time, partial 16S rRNA gene sequences were obtained for these morphospecies. Based on this gene, all of these strains clustered separately from other planktonic Anabaena and Aphanizomenon strains. They appeared in a cluster with Cylindrospermopsis Seenaya et Subba Raju and Raphidiopsis F. E. Fritsch et M. F. Rich, clustered closely together with two An. kisseleviana Elenkin strains available from GenBank. A new generic entity was defined (Sphaerospermum gen. nov., with the type species S. reniforme, based on the traditional species An. reniformis). These results contribute significantly to the knowledge base about genetic heterogeneity among planktonic Anabaena-like and Aphanizomenon-like morphospecies. Accordingly, the subgenus Dolichospermum, previously proposed for the group of planktonic Anabaena, should be revaluated. Secondary metabolite profiles of the An. reniformis and Aph. aphanizomenoides strains differed considerably from 17 other planktonic Anabaena strains of eight morphospecies isolated from Czech water bodies. Production of puwainaphycin A was found in both of the An. reniformis strains. Despite the relatively short phylogenetic distance from Cylidrospermopsis, the production of cylindrospermopsin was not detected in any of our strains.
authors.affiliation: University of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech Republic Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech RepublicBiology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech RepublicUniversity of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech Republic Institute of Microbiology, AS CR, Department of Autotrophic Microorganisms, Opatovický mlýn, CZ-379 81 Třeboň, Czech Republic Institute of Physical Biology, Zámek 136, CZ-37333, Nové Hrady, Czech RepublicBiology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Botany, ASCR, Dukelská 135, CZ-37982 Třeboň, Czech RepublicUniversity of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech Republic Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Botany, AS CR, Dukelská 135, CZ-37982 Třeboň, Czech Republic.
authors.forename: Eliška
authors.initials: E
authors.lastname: Zapomělová
authors.affiliation: University of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech Republic Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech RepublicBiology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech RepublicUniversity of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech Republic Institute of Microbiology, AS CR, Department of Autotrophic Microorganisms, Opatovický mlýn, CZ-379 81 Třeboň, Czech Republic Institute of Physical Biology, Zámek 136, CZ-37333, Nové Hrady, Czech RepublicBiology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Botany, ASCR, Dukelská 135, CZ-37982 Třeboň, Czech RepublicUniversity of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech Republic Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Botany, AS CR, Dukelská 135, CZ-37982 Třeboň, Czech Republic.
authors.forename: Jitka
authors.initials: J
authors.lastname: Jezberová
authors.affiliation: University of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech Republic Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech RepublicBiology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech RepublicUniversity of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech Republic Institute of Microbiology, AS CR, Department of Autotrophic Microorganisms, Opatovický mlýn, CZ-379 81 Třeboň, Czech Republic Institute of Physical Biology, Zámek 136, CZ-37333, Nové Hrady, Czech RepublicBiology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Botany, ASCR, Dukelská 135, CZ-37982 Třeboň, Czech RepublicUniversity of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech Republic Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Botany, AS CR, Dukelská 135, CZ-37982 Třeboň, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Hrouzek
authors.affiliation: University of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech Republic Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech RepublicBiology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech RepublicUniversity of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech Republic Institute of Microbiology, AS CR, Department of Autotrophic Microorganisms, Opatovický mlýn, CZ-379 81 Třeboň, Czech Republic Institute of Physical Biology, Zámek 136, CZ-37333, Nové Hrady, Czech RepublicBiology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Botany, ASCR, Dukelská 135, CZ-37982 Třeboň, Czech RepublicUniversity of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech Republic Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Botany, AS CR, Dukelská 135, CZ-37982 Třeboň, Czech Republic.
authors.forename: Daniel
authors.initials: D
authors.lastname: Hisem
authors.affiliation: University of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech Republic Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech RepublicBiology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech RepublicUniversity of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech Republic Institute of Microbiology, AS CR, Department of Autotrophic Microorganisms, Opatovický mlýn, CZ-379 81 Třeboň, Czech Republic Institute of Physical Biology, Zámek 136, CZ-37333, Nové Hrady, Czech RepublicBiology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Botany, ASCR, Dukelská 135, CZ-37982 Třeboň, Czech RepublicUniversity of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech Republic Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Botany, AS CR, Dukelská 135, CZ-37982 Třeboň, Czech Republic.
authors.forename: Klára
authors.initials: K
authors.lastname: Řeháková
authors.affiliation: University of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech Republic Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech RepublicBiology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech RepublicUniversity of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech Republic Institute of Microbiology, AS CR, Department of Autotrophic Microorganisms, Opatovický mlýn, CZ-379 81 Třeboň, Czech Republic Institute of Physical Biology, Zámek 136, CZ-37333, Nové Hrady, Czech RepublicBiology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Botany, ASCR, Dukelská 135, CZ-37982 Třeboň, Czech RepublicUniversity of South Bohemia, Faculty of Science, Branišovská 31, CZ-37005 České Budějovice, Czech Republic Biology Centre of AS CR, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic Institute of Botany, AS CR, Dukelská 135, CZ-37982 Třeboň, Czech Republic.
authors.forename: Jaroslava
authors.initials: J
authors.lastname: Komárková
keywords: 16S rRNA gene; Anabaena; cyanobacteria; morphology; new genus; phytoplankton; secondary metabolites; taxonomy