title: Role of extracellular signal‑regulated kinase 1/2 signal transduction pathway in insulin secretion by β‑TC6 cells.
abstract: The present study aimed to investigate the role of the extracellular signal-regulated kinase (ERK)1/2 signal transduction pathway in glucose‑stimulated insulin secretion in β‑TC6 mouse pancreatic cells. Insulin production by β‑TC6 cells was stimulated with various concentrations of glucose, which was dose-dependently inhibited by mitogen‑activated protein kinase inhibitor PD98059, as indicated by a radioimmunoassay. Furthermore, glucose stimulation enhanced the phosphorylation of ERK1/2, which was dose-dependently inhibited by PD98059, as indicated by western blot analysis. These results indicated that the activation of the ERK1/2 signal transduction pathway may have an important role in glucose‑stimulated insulin secretion in β‑TC6 cells.
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, P.R. China.
authors.forename: Ben
authors.initials: B
authors.lastname: Niu
authors.affiliation: Department of Cadre Ward, WISCO General Hospital, Wuhan, Hubei 430080, P.R. China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Endocrinology, The Affiliated Hospital of Kunming University of Science and Technology, The First People's Hospital of Yunnan, Kunming, Yunnan 650032, P.R. China.
authors.forename: Heng
authors.initials: H
authors.lastname: Su
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, P.R. China.
authors.forename: Xueshan
authors.initials: X
authors.lastname: Xia
authors.affiliation: Department of Endocrinology, The Affiliated Hospital of Kunming University of Science and Technology, The First People's Hospital of Yunnan, Kunming, Yunnan 650032, P.R. China.
authors.forename: Qiu
authors.initials: Q
authors.lastname: He
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, P.R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Department of Endocrinology, The Affiliated Hospital of Kunming University of Science and Technology, The First People's Hospital of Yunnan, Kunming, Yunnan 650032, P.R. China.
authors.forename: Yuanming
authors.initials: Y
authors.lastname: Xue
authors.affiliation: Institute of Basic and Clinical Medicine, Center of Clinical Molecular Biology of Yunnan, The Affiliated Hospital of Kunming University of Science and Technology, The First People's Hospital of Yunnan, Kunming, Yunnan 650032, P.R. China.
authors.forename: Xinming
authors.initials: X
authors.lastname: Yan
mesh_terms: D000818:Animals; D002460:Cell Line; D005419:Flavonoids / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D020935:MAP Kinase Signaling System / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D048052:Mitogen-Activated Protein Kinase 3 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010179:Pancreas / Q000166:cytology / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects / Q000502:physiology
chemical_list: D005419:Flavonoids; D007328:Insulin; D048052:Mitogen-Activated Protein Kinase 3; D005947:Glucose; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one