title: Use of Protein Folding Reagents.
abstract: The reagents and methods for purification and use of the most commonly used denaturants, guanidine hydrochloride (guanidine-HCl) and urea, are described. Other protein denaturants and reagents used to fold proteins are briefly mentioned. Sulfhydryl reagents (reducing agents) and "oxido-shuffling" (or oxidative regeneration) systems are also described.
mesh_terms: D001671:Biochemistry / Q000379:methods*; D019791:Guanidine / Q000737:chemistry; D007202:Indicators and Reagents / Q000737:chemistry*; D007700:Kinetics; D010084:Oxidation-Reduction; D011489:Protein Denaturation; D017510:Protein Folding; D055550:Protein Stability; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D019163:Reducing Agents / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013439:Sulfhydryl Reagents / Q000737:chemistry; D014508:Urea / Q000737:chemistry
chemical_list: D007202:Indicators and Reagents; D011506:Proteins; D019163:Reducing Agents; D012997:Solvents; D013439:Sulfhydryl Reagents; D014508:Urea; D019791:Guanidine
keywords: guanidine hydrochloride; oxidative regeneration; protein denaturants; protein folding; sulfhydryl reagents; urea