title: Disturbance of firefly luciferase-based bioassays by different aluminum species.
abstract: Luciferase-dependent assays, important for biochemical analyses of cytotoxicity and reporter genes, may be perturbed by compounds interfering with the luciferase reaction. We analyzed the impact of different aluminum (Al) species on a luciferase-based assay for determination of cellular adenosine triphosphate. Al(0) nanoparticles (Al(0)-NPs) but not Al2O3-NPs decreased luminescence, correlated to high absorbance of Al(0)-NPs. By contrast, Al ions increased the luminescent signal. Data demonstrate that luciferase-dependent assays can be reciprocally disturbed by Al-NPs or Al ions in a specific manner, depending on the particular Al species. Careful interpretation of data from such experiments is essential in order to obtain conclusive results.
authors.affiliation: Department of Food Safety, Federal Institute for Risk Assessment, 10589 Berlin, Germany.
authors.forename: Caroline
authors.initials: C
authors.lastname: Lehmann
authors.affiliation: Department of Food Safety, Federal Institute for Risk Assessment, 10589 Berlin, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Sieg
authors.affiliation: Department of Food Safety, Federal Institute for Risk Assessment, 10589 Berlin, Germany.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Lampen
authors.affiliation: Department of Food Safety, Federal Institute for Risk Assessment, 10589 Berlin, Germany. Electronic address: albert.braeuning@bfr.bund.de.
authors.forename: Albert
authors.initials: A
authors.lastname: Braeuning
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D000535:Aluminum / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D000537:Aluminum Oxide / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001681:Biological Assay; D018938:Caco-2 Cells; D016923:Cell Death / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D049409:Luciferases, Firefly / Q000235:genetics / Q000378:metabolism*; D008163:Luminescent Measurements; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D000255:Adenosine Triphosphate; D000535:Aluminum; D049409:Luciferases, Firefly; D000537:Aluminum Oxide
keywords: ATP determination; Caco-2 cells; Cytotoxicity testing; Nanoparticle; Reporter assay