title: Impact of oral consumption of heat-treated Bacteroides xylanisolvens DSM 23964 on the level of natural TFα-specific antibodies in human adults.
abstract: It is now generally accepted that the human body exists in close synergy with the gut microbiome and that this cross-talk plays an essential role in human health and disease. One facet from the many interactions between the microbiome and the immune system is the induction of natural antibodies to commensal bacterial glycans, such as blood group antigens, the alpha-Gal epitope or the Thomsen-Friedenreich (TFα) antigen. Since we have observed that certain species of the commensal genus Bacteroides express the TFα antigen, we examined whether the oral dietary supplementation of a pasteurised Bacteroides xylanisolvens strain might be able to enhance the level of natural anti-TFα antibodies in healthy adults. The data obtained from a double-blind, placebo-controlled study involving 140 healthy volunteers and lasting 8 weeks revealed that the oral uptake of this strain was indeed able to increase the level of TFα-specific immunoglobulin M serum antibodies. The effect was dose-dependent but remained - at any doses - within the physiological range determined before intervention. Furthermore, the effect reverted after stopping the intake. The results support the idea of the microbiome inducing the generation of systemic antigen-specific antibodies against sugar epitopes. They also demonstrate the possibility to modulate essential regulatory or defence processes through dietary supplementation of selected commensal bacteria with the aim to assist human health.
authors.affiliation: 1 Avitop GmbH, Robert-Roessler-Str. 10, 13125 Berlin, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Ulsemer
authors.affiliation: 1 Avitop GmbH, Robert-Roessler-Str. 10, 13125 Berlin, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Toutounian
authors.affiliation: 2 Leibniz University of Hannover, Institute of Food Science and Human Nutrition, Am Kleinen Felde 30, 30163 Hannover, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Kressel
authors.affiliation: 3 Glycotope GmbH, Robert-Roessle-Str. 10, 13125 Berlin, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Goletz
authors.affiliation: 1 Avitop GmbH, Robert-Roessler-Str. 10, 13125 Berlin, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Schmidt
authors.affiliation: 3 Glycotope GmbH, Robert-Roessle-Str. 10, 13125 Berlin, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Karsten
authors.affiliation: 2 Leibniz University of Hannover, Institute of Food Science and Human Nutrition, Am Kleinen Felde 30, 30163 Hannover, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Hahn
authors.affiliation: 3 Glycotope GmbH, Robert-Roessle-Str. 10, 13125 Berlin, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Goletz
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000906:Antibodies / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000276:immunology*; D001439:Bacteroides / Q000276:immunology*; D045744:Cell Line, Tumor; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology; D008297:Male; D008875:Middle Aged; D055815:Young Adult
chemical_list: D000906:Antibodies; D000942:Antigens, Bacterial; D015295:Antigens, Tumor-Associated, Carbohydrate; D007075:Immunoglobulin M; C016467:Thomsen-Friedenreich antigen; C025991:Thomsen-Friedenreich antibodies
keywords: Bacteroides xylanisolvens; TFα-specific antibodies; Thomsen-Friedenreich antigen; immune response; microbiome