title: Developmental potential of clinically discarded human embryos and associated chromosomal analysis.
abstract: Clinically discarded human embryos, which are generated from both normal and abnormal fertilizations, have the potential of developing into blastocysts. A total of 1,649 discarded human embryos, including zygotes containing normal (2PN) and abnormal (0PN, 1PN, 3PN and ≥4PN) pronuclei and prematurely cleaved embryos (2Cell), were collected for in vitro culture to investigate their developmental potential and chromosomal constitution using an SNP array-based chromosomal analysis. We found that blastocyst formation rates were 63.8% (for 2Cell embryos), 22.6% (2PN), 16.7% (0PN), 11.2% (3PN) and 3.6% (1PN). SNP array-based chromosomal analysis of the resultant blastocysts revealed that the percentages of normal chromosomes were 55.2% (2Cell), 60.7% (2PN), 44.4% (0PN) and 47.4% (0PN). Compared with clinical preimplantation genetic diagnosis (PGD) data generated with clinically acceptable embryos, results of the SNP array-based chromosome analysis on blastocysts from clinically discarded embryos showed similar values for the frequency of abnormal chromosome occurrence, aberrant signal classification and chromosomal distribution. The present study is perhaps the first systematic analysis of the developmental potential of clinically discarded embryos and provides a basis for future studies.
authors.affiliation: Center for Reproductive Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Guidong
authors.initials: G
authors.lastname: Yao
authors.affiliation: Center for Reproductive Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Xu
authors.affiliation: Center for Reproductive Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Xin
authors.affiliation: Center for Reproductive Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Niu
authors.affiliation: Center for Reproductive Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Senlin
authors.initials: S
authors.lastname: Shi
authors.affiliation: Center for Reproductive Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Haixia
authors.initials: H
authors.lastname: Jin
authors.affiliation: Center for Reproductive Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Song
authors.affiliation: Center for Reproductive Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Enyin
authors.initials: E
authors.lastname: Wang
authors.affiliation: Center for Reproductive Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Qingling
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Center for Reproductive Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.affiliation: Center for Reproductive Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Yingpu
authors.initials: Y
authors.lastname: Sun
mesh_terms: D001706:Biopsy; D001755:Blastocyst / Q000166:cytology; D002467:Cell Nucleus / Q000378:metabolism; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000648:ultrastructure*; D054885:Cumulus Cells / Q000166:cytology; D046149:Embryo Culture Techniques / Q000379:methods*; D047108:Embryonic Development; D005260:Female; D005306:Fertilization; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D009865:Oocytes / Q000166:cytology; D020641:Polymorphism, Single Nucleotide; D019836:Preimplantation Diagnosis; D015053:Zygote