title: Enzyme-Free Dissociation of Neurospheres by a Microfluidic Chip-Based Method.
abstract: Neurosphere assay is a common and robust method for identification of neural stem/progenitor cells, but obtaining large numbers of live single cells from dissociated neurospheres is difficult using nonenzymatic methods. Here, we present an enzyme-free method for high-efficiency neurosphere dissociation into single cells using microfluidic device technology. This method allows single cell dissociation of DC115 and KT98 cells with high cell viabilities (80-85 %), single-cell yield (91-95 %), and recovery (75-93 %).
authors.affiliation: National Health Research Institutes, 35, Keyan Road, Zhunan Town, Miaoli County, 35053, Taiwan.
authors.forename: Ching-Hui
authors.initials: CH
authors.lastname: Lin
authors.affiliation: National Health Research Institutes, 35, Keyan Road, Zhunan Town, Miaoli County, 35053, Taiwan.
authors.forename: Hao-Chen
authors.initials: HC
authors.lastname: Chang
authors.affiliation: National Health Research Institutes, 35, Keyan Road, Zhunan Town, Miaoli County, 35053, Taiwan.
authors.forename: Don-Ching
authors.initials: DC
authors.lastname: Lee
authors.affiliation: National Health Research Institutes, 35, Keyan Road, Zhunan Town, Miaoli County, 35053, Taiwan.
authors.forename: Ing-Ming
authors.initials: IM
authors.lastname: Chiu
authors.affiliation: National Health Research Institutes, 35, Keyan Road, Zhunan Town, Miaoli County, 35053, Taiwan. chsu@nhri.org.tw.
authors.forename: Chia-Hsien
authors.initials: CH
authors.lastname: Hsu
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000235:genetics; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D044085:Microfluidics / Q000379:methods*; D058953:Neural Stem Cells / Q000166:cytology*
keywords: BioMEMS; Enzyme-free dissociation; Lab-on-a-chip; Microfluidics; Neurospheres; Single cells