title: Novel Bioreactor Platform for Scalable Cardiomyogenic Differentiation from Pluripotent Stem Cell-Derived Embryoid Bodies.
abstract: Generation of cardiomyocytes from pluripotent stem cells (PSCs) is a common and valuable approach to produce large amount of cells for various applications, including assays and models for drug development, cell-based therapies, and tissue engineering. All these applications would benefit from a reliable bioreactor-based methodology to consistently generate homogenous PSC-derived embryoid bodies (EBs) at a large scale, which can further undergo cardiomyogenic differentiation. The goal of this chapter is to describe a scalable method to consistently generate large amount of homogeneous and synchronized EBs from PSCs. This method utilizes a slow-turning lateral vessel bioreactor to direct the EB formation and their subsequent cardiomyogenic lineage differentiation.
authors.affiliation: Department of Preclinical and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, 73170, Thailand. sasitorn.run@mahidol.edu.
authors.forename: Sasitorn
authors.initials: S
authors.lastname: Rungarunlert
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, National University of Singapore, 11 Lower Kent Ridge Road, Singapore, 119083, Singapore.
authors.forename: Joao N
authors.initials: JN
authors.lastname: Ferreira
authors.affiliation: BioTalentum Ltd., Aulich Lajos u. 26, Gödöllö, 2100, Hungary.|Molecular Animal Biotechnology Laboratory, Szent Istvan University, Pater K. u. 1, Gödöllö, 2100, Hungary.
authors.forename: Andras
authors.initials: A
authors.lastname: Dinnyes
mesh_terms: D000818:Animals; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002454:Cell Differentiation; D002460:Cell Line; D058732:Embryoid Bodies / Q000166:cytology*; D004867:Equipment Design; D051379:Mice; D032383:Myocytes, Cardiac / Q000166:cytology*; D039904:Pluripotent Stem Cells / Q000166:cytology; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods
keywords: Bioreactor; Cardiomyocyte; Embryoid body; Pluripotent stem cells; Slow turning lateral vessel