title: Comparison of molecular and microscopic technique for detection of Theileria annulata from the field cases of cattle.
abstract: OBJECTIVE
  Tropical theileriosis is fatal hemoprotozoal disease of dairy animals caused by Theileria annulata. The aim of the present study was to detect the T. annulata and comparison of results of molecular and microscopic techniques.
  
  
  METHODS
  A total of 52 blood samples were collected from the cattle suspected for theileriosis across the Banaskantha district. All the samples were screened for theileriosis using Giemsa's staining technique and polymerase chain reaction (PCR).
  
  
  RESULTS
  Total of 17 (32.69%) and 24 (46.15%) samples were found positive for theileriosis by microscopic examination and PCR test, respectively. It revealed that the study area is endemic for theileriosis, and the microscopic technique has 70.83% sensitivity and 100% specificity with respect to PCR technique.
  
  
  CONCLUSIONS
  It may be concluded from the present study that the PCR is comparatively sensitive technique than microscopic examination and may be recommended to use in the field for screening of theileriosis in the study area, where a high prevalence of diseases have been reported due to intensive dairy farming.
authors.affiliation: Division of Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar - 385 506, Gujarat, India.
authors.forename: H C
authors.initials: HC
authors.lastname: Chauhan
authors.affiliation: Division of Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar - 385 506, Gujarat, India.
authors.forename: B K
authors.initials: BK
authors.lastname: Patel
authors.affiliation: Division of Veterinary Microbiology, College of Veterinary Science & Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar - 385 506, Gujarat, India.
authors.forename: A G
authors.initials: AG
authors.lastname: Bhagat
authors.affiliation: Division of Veterinary Microbiology, College of Veterinary Science & Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar - 385 506, Gujarat, India.
authors.forename: M V
authors.initials: MV
authors.lastname: Patel
authors.affiliation: Division of Veterinary Microbiology, College of Veterinary Science & Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar - 385 506, Gujarat, India.
authors.forename: S I
authors.initials: SI
authors.lastname: Patel
authors.affiliation: Division of Veterinary Pathology, College of Veterinary Science & Animal House, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar - 385 506, Gujarat, India.
authors.forename: S H
authors.initials: SH
authors.lastname: Raval
authors.affiliation: Teaching Veterinary Clinical Complex (College Clinics), College of Veterinary Science & Animal House, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar - 385 506, Gujarat, India.
authors.forename: H H
authors.initials: HH
authors.lastname: Panchasara
authors.affiliation: Division of Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar - 385 506, Gujarat, India.
authors.forename: M D
authors.initials: MD
authors.lastname: Shrimali
authors.affiliation: Division of Veterinary Microbiology, College of Veterinary Science & Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar - 385 506, Gujarat, India.
authors.forename: A C
authors.initials: AC
authors.lastname: Patel
authors.affiliation: Division of Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar - 385 506, Gujarat, India.
authors.forename: B S
authors.initials: BS
authors.lastname: Chandel
keywords: Giemsa staining; polymerase chain reaction; theileriosis