title: Aqueous leaf extract of Passiflora alata Curtis promotes antioxidant and anti-inflammatory effects and consequently preservation of NOD mice beta cells (non-obese diabetic).
abstract: Passiflora alata Curtis (P. alata) leaves have anti-inflammatory properties; the present study aimed to investigate the anti-diabetogenic properties of P. alata aqueous leaf extract. HPLC analysis identified the phenolic compounds catechin, epicatechin and rutin. The aqueous extract was administered for 30weeks to non-obese diabetic (NOD) mice presenting a decrease of 28.6% in diabetes incidence and the number of inflammatory cells in pancreatic islets, when compared with the control group (water). The P. alata group presented an antioxidant effect and decreased lipid peroxidation in the serum of NOD mice. Increased numbers of insulin-positive cells were also observed in the pancreatic islets of the treated group. The diabetic group exhibited higher levels in the glucose tolerance test and glycemic index, in comparison to the P. alata-treated group and non-diabetic control BALB/c mice. In addition, the P. alata extract reduced the percentage and the proliferation index of NOD mice lymphocytes submitted to in vitro dose/response mitogenic stimulation assays. These results suggest that the aqueous extract of P. alata has anti-inflammatory properties, contributing to the protection of beta cells in pancreatic islets in NOD mice, and presents potential for use a supporting approach to treat type 1 diabetes.
authors.affiliation: Laboratory of Translational Immunology, Department of Internal Medicine, Faculty of Medical Sciences, University of Campinas, Rua Vital Brasil 300, Cidade Universitária Zeferino Vaz, 13083-887 Campinas, SP, Brazil.
authors.forename: D
authors.initials: D
authors.lastname: Figueiredo
authors.affiliation: Laboratory of Translational Immunology, Department of Internal Medicine, Faculty of Medical Sciences, University of Campinas, Rua Vital Brasil 300, Cidade Universitária Zeferino Vaz, 13083-887 Campinas, SP, Brazil.
authors.forename: Talita Cristina
authors.initials: TC
authors.lastname: Colomeu
authors.affiliation: Laboratory of Translational Immunology, Department of Internal Medicine, Faculty of Medical Sciences, University of Campinas, Rua Vital Brasil 300, Cidade Universitária Zeferino Vaz, 13083-887 Campinas, SP, Brazil.
authors.forename: Nayara Simon Gonzalez
authors.initials: NSG
authors.lastname: Schumacher
authors.affiliation: Laboratory of Translational Immunology, Department of Internal Medicine, Faculty of Medical Sciences, University of Campinas, Rua Vital Brasil 300, Cidade Universitária Zeferino Vaz, 13083-887 Campinas, SP, Brazil.
authors.forename: L G
authors.initials: LG
authors.lastname: Stivanin-Silva
authors.affiliation: Department of Food and Nutrition, School of Food Engineering, University of Campinas, Rua Monteiro Lobato 80, Cidade Universitária Zeferino Vaz, 13083-862 Campinas, SP, Brazil.
authors.forename: Cinthia Baú Betim
authors.initials: CBB
authors.lastname: Cazarin
authors.affiliation: Agronomic Institute of Campinas, Av. Theodureto de Almeida Camargo 1500, Vila Nova, 13012-970 Campinas, SP, Brazil.
authors.forename: Laura Maria Molina
authors.initials: LMM
authors.lastname: Meletti
authors.affiliation: Laboratory of Translational Immunology, Department of Internal Medicine, Faculty of Medical Sciences, University of Campinas, Rua Vital Brasil 300, Cidade Universitária Zeferino Vaz, 13083-887 Campinas, SP, Brazil.
authors.forename: Luís Gustavo Romani
authors.initials: LGR
authors.lastname: Fernandes
authors.affiliation: Department of Food and Nutrition, School of Food Engineering, University of Campinas, Rua Monteiro Lobato 80, Cidade Universitária Zeferino Vaz, 13083-862 Campinas, SP, Brazil.
authors.forename: Marcelo Alexandre
authors.initials: MA
authors.lastname: Prado
authors.affiliation: Laboratory of Translational Immunology, Department of Internal Medicine, Faculty of Medical Sciences, University of Campinas, Rua Vital Brasil 300, Cidade Universitária Zeferino Vaz, 13083-887 Campinas, SP, Brazil; Department of Internal Medicine, Faculty of Medical Sciences, Rua Alexandre Fleming 09, University of Campinas, Campinas, SP, Brazil. Electronic address: zollner@unicamp.br.
authors.forename: R L
authors.initials: RL
authors.lastname: Zollner
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D000975:Antioxidants / Q000627:therapeutic use*; D002478:Cells, Cultured; D019610:Cytoprotection; D003922:Diabetes Mellitus, Type 1 / Q000188:drug therapy*; D005260:Female; D006801:Humans; D007328:Insulin / Q000097:blood; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000502:physiology; D015227:Lipid Peroxidation / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D016688:Mice, Inbred NOD; D029598:Passiflora / Q000276:immunology*; D010936:Plant Extracts / Q000627:therapeutic use*; D018515:Plant Leaves
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D007328:Insulin; D010936:Plant Extracts
keywords: Anti-inflammatory effect; NOD mice; Passiflora alata Curtis; Type 1 diabetes mellitus