title: Salmonella flagellin acted as an effective fusion partner for expression of Plasmodium falciparum surface protein 25 in Escherichia coli.
abstract: Plasmodium falciparum surface protein 25 (Pfs25) is a hard-to-express and hard-to-solubilize protein in Escherichia coli. To overcome this problem, the phase 1 flagellin of Salmonella enterica serovar Typhimurium (FliC) was used as a fusion partner for Pfs25. The fusion expression of Pfs25 with FliC greatly enhanced the expression level and solubility of Pfs25 in E. coli BL21(DE3). The Ni-purified fusion protein of FliC-Pfs25 was recognized by two anti-Pfs25 monoclonal antibodies. By comparison, it was shown that the Pfs25 within FliC-Pfs25 contained epitopes similar or identical to those on Pichia pastoris-produced Pfs25. The data obtained from this study demonstrated that the fusion with Salmonella flagellin greatly improved the expression of Pfs25 in E. coli.
authors.affiliation: a Department of Rheumatology and Immunology , Shanghai Changzheng Hospital, Second Military Medical University , Shanghai , China.
authors.forename: Feng
authors.initials: F
authors.lastname: Qian
authors.affiliation: a Department of Rheumatology and Immunology , Shanghai Changzheng Hospital, Second Military Medical University , Shanghai , China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Li
authors.affiliation: b Fourth Research Laboratory, Lanzhou Institute of Biological Products Co., Ltd. , Lanzhou , China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: b Fourth Research Laboratory, Lanzhou Institute of Biological Products Co., Ltd. , Lanzhou , China.
authors.forename: Lin
authors.initials: L
authors.lastname: Jiang
authors.affiliation: a Department of Rheumatology and Immunology , Shanghai Changzheng Hospital, Second Military Medical University , Shanghai , China.
authors.forename: Huji
authors.initials: H
authors.lastname: Xu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000913:Antibodies, Protozoan / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005408:Flagellin / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D008807:Mice, Inbred BALB C; D010963:Plasmodium falciparum / Q000235:genetics / Q000276:immunology*; D015800:Protozoan Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D012486:Salmonella typhimurium / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000939:Epitopes; C071284:Pfs25 protein, Plasmodium falciparum; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins; D005408:Flagellin
keywords: Escherichia coli; Fusion partner; Pfs25; Plasmodium falciparum; Salmonella flagellin