title: In Vitro Culture of Human Hematopoietic Stem Cells in Serum Free Medium and Their Monitoring by Flow Cytometry.
abstract: Hematopoietic stem cells can be isolated from human blood cells trapped in leukoreduction systems. The leukoreduction systems filters or chambers are usually discarded from routine blood or platelet donations in blood banks around the world. These CD34+ cells are a good source of normal stem cells and can be used as models to characterize the blood stem cells before and after culture in vitro. This chapter contains detailed methodologies for the isolation of stem cells from peripheral blood, the culture of these cells in a medium exempt of animal proteins and for the flow cytometry analysis of the resulting cell population for the characterization of their differentiation.
authors.affiliation: Recherche et Développement, Héma-Québec, 1070 avenue des Sciences-de-la-vie, Québec, QC, Canada, G1V 5C3.
authors.forename: Marc
authors.initials: M
authors.lastname: Cloutier
authors.affiliation: Recherche et Développement, Héma-Québec, 1070 avenue des Sciences-de-la-vie, Québec, QC, Canada, G1V 5C3.|Faculté des sciences et de génie, Biochimie, Microbiologie et Bio-informatique, Université Laval, Québec, QC, Canada.
authors.forename: Christine
authors.initials: C
authors.lastname: Jobin
authors.affiliation: Recherche et Développement, Héma-Québec, 1070 avenue des Sciences-de-la-vie, Québec, QC, Canada, G1V 5C3.
authors.forename: Carl
authors.initials: C
authors.lastname: Simard
authors.affiliation: Recherche et Développement, Héma-Québec, 1070 avenue des Sciences-de-la-vie, Québec, QC, Canada, G1V 5C3. sonia.neron@hema-quebec.qc.ca.|Faculté des sciences et de génie, Biochimie, Microbiologie et Bio-informatique, Université Laval, Québec, QC, Canada. sonia.neron@hema-quebec.qc.ca.
authors.forename: Sonia
authors.initials: S
authors.lastname: Néron
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000235:genetics; D058948:Cell Tracking / Q000379:methods*; D016895:Culture Media, Serum-Free; D005434:Flow Cytometry / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans
chemical_list: D016895:Culture Media, Serum-Free
keywords: Adult CD34+ cells; Animal protein free medium; Flow cytometry; Hematopoietic progenitors; Multiparametric methods; SPADE; Stem cell expansion