title: EFFECT OF USE OF BONE-MARROW CENTRIFUGATE ON MUSCLE INJURY TREATMENT: EXPERIMENTAL STUDY ON RABBITS.
abstract: OBJECTIVE
  The objective of this study was to evaluate the effect of bone-marrow centrifugate on the healing of muscle injuries in rabbits.
  
  
  METHODS
  This experimental study involved use of fifteen adult male New Zealand White rabbits. Each animal received a transverse lesion in the middle of the right tibialis anterior muscle, to which an absorbable collagen sponge, soaked in a centrifugate of bone marrow aspirate from the ipsilateral iliac bone, was added. The left hind limb was used as a control and underwent the same injury, but in this case only the absorbable collagen sponge. Thirty days later, the animals were sacrificed to study the muscle healing. These muscle areas were subjected to histological analysis with histomorphometry, with the aim of measuring the number of muscle cells per square micrometer undergoing regeneration and the proportion of resultant fibrosis.
  
  
  RESULTS
  The centrifugation method used in this study resulted in an average concentration of nucleated cells greater than the number of these cells in original aspirates, without causing significant cell destruction. Addition of the bone marrow centrifugate did not result in any significant increase in the number of muscle cells undergoing regeneration, in relation to the control group. There was also no significant difference in the proportion of resultant fibrosis, compared with the control group.
  
  
  CONCLUSIONS
  Administration of the bone marrow centrifugate used in this study did not favor healing of muscle injuries in rabbits.
authors.affiliation: Orthopedics and Traumatology, Master's degree in Medicine and Health Sciences at the Universidade Estadual de Londrina - Londrina, PR, Brazil.
authors.forename: Daniel Ferreira Fernandes
authors.initials: DF
authors.lastname: Vieira
authors.affiliation: Associate Professor of the School of Medicine of Universidade de São Paulo - São Paulo, SP, Brazil.
authors.forename: Roberto
authors.initials: R
authors.lastname: Guarniero
authors.affiliation: Doctor and Adjunct Professor of the Orthopedics and Traumatology Discipline of Universidade Estadual de Londrina - Londrina, PR, Brazil.
authors.forename: Carlos Eduardo Sanches
authors.initials: CE
authors.lastname: Vaz
authors.affiliation: Doctor and Adjunct Professor of the Orthopedics and Traumatology Discipline of Universidade Estadual de Londrina - Londrina, PR, Brazil.
authors.forename: Paulo José
authors.initials: PJ
authors.lastname: de Santana
keywords: Animals Models; Bone Marrow; Rabbits; Regeneration; Skeletal Muscle; Stem Cells