title: High-fat diet exacerbates pain-like behaviors and periarticular bone loss in mice with CFA-induced knee arthritis.
abstract: Our aim was to quantify nociceptive spontaneous behaviors, knee edema, proinflammatory cytokines, bone density, and microarchitecture in high-fat diet (HFD)-fed mice with unilateral knee arthritis.
  
  
  
  ICR male mice were fed either standard diet (SD) or HFD starting at 3 weeks old. At 17 weeks, HFD and SD mice received intra-articular injections either with Complete Freund's Adjuvant (CFA) or saline into the right knee joint every 7 days for 4 weeks. Spontaneous pain-like behaviors and knee edema were assessed for 26 days. At day 26 post-first CFA injection, serum levels of IL-1β, IL-6, and RANKL were measured by ELISA, and microcomputed tomography analysis of knee joints was performed.
  
  
  
  HFD-fed mice injected with CFA showed greater spontaneous pain-like behaviors of the affected extremity as well as a decrease in the weight-bearing index compared to SD-fed mice injected with CFA. Knee edema was not significantly different between diets. HFD significantly exacerbated arthritis-induced bone loss at the distal femoral metaphysis but had no effect on femoral diaphyseal cortical bone. HFD did not modify serum levels of proinflammatory cytokines.
  
  
  
  HFD exacerbates pain-like behaviors and significantly increases the magnitude of periarticular trabecular bone loss in a murine model of unilateral arthritis.
authors.affiliation: Unidad Académica Multidisciplinaria Reynosa-Aztlán, UAT, Reynosa, Tamaulipas, México.
authors.forename: Aleyda A
authors.initials: AA
authors.lastname: Loredo-Pérez
authors.affiliation: Unidad Académica Multidisciplinaria Reynosa-Aztlán, UAT, Reynosa, Tamaulipas, México.
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Montalvo-Blanco
authors.affiliation: Unidad Académica Multidisciplinaria Reynosa-Aztlán, UAT, Reynosa, Tamaulipas, México.
authors.forename: Luis I
authors.initials: LI
authors.lastname: Hernández-González
authors.affiliation: Productos Medix, S.A. de C.V, México City, México.
authors.forename: Maricruz
authors.initials: M
authors.lastname: Anaya-Reyes
authors.affiliation: Productos Medix, S.A. de C.V, México City, México.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Fernández Del Valle-Laisequilla
authors.affiliation: Sección De Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, México City, México.
authors.forename: Juan G
authors.initials: JG
authors.lastname: Reyes-García
authors.affiliation: Unidad Académica Multidisciplinaria Reynosa-Aztlán, UAT, Reynosa, Tamaulipas, México.
authors.forename: Rosa I
authors.initials: RI
authors.lastname: Acosta-González
authors.affiliation: Unidad Académica Multidisciplinaria Reynosa-Aztlán, UAT, Reynosa, Tamaulipas, México.
authors.forename: Arisai
authors.initials: A
authors.lastname: Martínez-Martínez
authors.affiliation: Unidad Académica Multidisciplinaria Reynosa-Aztlán, UAT, Reynosa, Tamaulipas, México.
authors.forename: Jaira C
authors.initials: JC
authors.lastname: Villarreal-Salcido
authors.affiliation: Unidad Académica Multidisciplinaria Reynosa-Aztlán, UAT, Reynosa, Tamaulipas, México.
authors.forename: Virginia M
authors.initials: VM
authors.lastname: Vargas-Muñoz
authors.affiliation: Departamento de Biología Celular, Instituto Nacional de Perinatología, Secretaría de Salud, México City, México.
authors.forename: Enriqueta
authors.initials: E
authors.lastname: Muñoz-Islas
authors.affiliation: Unidad Académica Multidisciplinaria Reynosa-Aztlán, UAT, Reynosa, Tamaulipas, México.
authors.forename: Martha B
authors.initials: MB
authors.lastname: Ramírez-Rosas
authors.affiliation: Unidad Académica Multidisciplinaria Reynosa-Aztlán, UAT, Reynosa, Tamaulipas, México.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Jiménez-Andrade
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000097:blood / Q000000981:diagnostic imaging / Q000503:physiopathology*; D001522:Behavior, Animal / Q000502:physiology*; D015519:Bone Density; D059305:Diet, High-Fat / Q000009:adverse effects*; D004487:Edema; D005620:Freund's Adjuvant; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D007719:Knee Joint / Q000000981:diagnostic imaging; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010146:Pain / Q000503:physiopathology*; D053245:RANK Ligand / Q000097:blood; D055114:X-Ray Microtomography
chemical_list: D053583:Interleukin-1beta; D015850:Interleukin-6; D053245:RANK Ligand; C505689:Tnfsf11 protein, mouse; D005620:Freund's Adjuvant