title: A Fluorescence Immunochromatographic Assay Using Europium (III) Chelate Microparticles for Rapid, Quantitative and Sensitive Detection of Creatine Kinase MB.
abstract: The isoenzyme creatine kinase MB is very important for diagnosis of acute myocardial infarction (AMI). Some CK-MB immunoassays are sensitive, accurate and available for clinical application, but they are expensive and time-consuming procedures. Furthermore, conventional fluorescence immunochromatographic assays (FL-ICAs) have suffered from background fluorescence interference and low analytical sensitivity. A rapid and simple FL-ICA with Eu (III) chelate polystyrene microparticles was developed to determine CK-MB in 50uL serum samples using a portable test strip reader by measuring the fluorescence peak heights of the test line (HT) and the control line (HC) in 12 min. The assay was reliable with a good correlation coefficient between HT/HC ratio and CK-MB concentration in samples. A linear range was 0.85-100.29 ng/mL for CK-MB, and the LOD was 0.029 ng/mL. The intra- and inter-assay coefficients of variation (CV) were both <10 % and the average recoveries were from 90.17 % -112.63 % for CK-MB. The system performed well in interference experiments. Furthermore, a highly significant correlation (r = 0.9794, P < 0.001) between this method and the commercially available bioMérieux mini VIDAS system were attained for measuring 120 CK-MB samples. These results indicated that the Eu (III) chelate microparticles-based FL-ICA is simple, fast, highly sensitive, reliable, and reproducible for point-of-care testing of CK-MB concentrations in serum. Graphical Abstract ᅟ.
authors.affiliation: Department of Laboratory Medicine, Guangzhou General Hospital of Guangzhou Military Command of PLA, No.111, Liuhua Road, Yuexiu District, Guangzhou, Guangdong, China.|Guangzhou University of Chinese Medicine, No.12, Airport Road, Baiyun District, Guangzhou, Guangdong, China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Lai
authors.affiliation: State Key Laboratory of Organ Failure, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Biotechnology, Southern Medical University, No.1838, Guangzhou Avenue North, Guangzhou, Guangdong, People's Republic of China.
authors.forename: Rong-Liang
authors.initials: RL
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Organ Failure, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Biotechnology, Southern Medical University, No.1838, Guangzhou Avenue North, Guangzhou, Guangdong, People's Republic of China.
authors.forename: Tian-Cai
authors.initials: TC
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Organ Failure, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Biotechnology, Southern Medical University, No.1838, Guangzhou Avenue North, Guangzhou, Guangdong, People's Republic of China.
authors.forename: Zhi-Ning
authors.initials: ZN
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Organ Failure, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Biotechnology, Southern Medical University, No.1838, Guangzhou Avenue North, Guangzhou, Guangdong, People's Republic of China. wg@smu.edu.cn.
authors.forename: Ying-Song
authors.initials: YS
authors.lastname: Wu
authors.affiliation: Department of Laboratory Medicine, Guangzhou General Hospital of Guangzhou Military Command of PLA, No.111, Liuhua Road, Yuexiu District, Guangzhou, Guangdong, China. mature303@126.com.
authors.forename: Lin-Hai
authors.initials: LH
authors.lastname: Li
mesh_terms: D002614:Chelating Agents / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D052279:Creatine Kinase, MB Form / Q000378:metabolism*; D005063:Europium / Q000737:chemistry*; D008863:Microspheres; D011137:Polystyrenes / Q000737:chemistry; D011934:Reagent Strips / Q000737:chemistry; D012016:Reference Values; D013050:Spectrometry, Fluorescence; D013997:Time Factors
chemical_list: D002614:Chelating Agents; D011137:Polystyrenes; D011934:Reagent Strips; D005063:Europium; D052279:Creatine Kinase, MB Form
keywords: Acute myocardial infarction; Creatine kinase MB; Eu (III) chelate microparticles; Fluorescence immunochromatographic assay