title: Phylogenetic analysis, genetic diversity and relationships between the recently segregated species of Corynandra and Cleoserrata from the genus Cleome using DNA barcoding and molecular markers.
abstract: Cleome is the largest genus in the family Cleomaceae and it is known for its various medicinal properties. Recently, some species from the Cleome genus (Cleome viscosa, Cleome chelidonii, Cleome felina and Cleome speciosa) are split into genera Corynandra (Corynandra viscosa, Corynandra chelidonii, Corynandra felina), and Cleoserrata (Cleoserrata speciosa). The objective of this study was to obtain DNA barcodes for these species for their accurate identification and determining phylogenetic relationships. Out of 10 screened barcoding regions, rbcL, matK and ITS1 regions showed higher PCR efficiency and sequencing success. This study added matK, rbcL and ITS1 barcodes for the identification of Corynandra chelidonii, Corynandra felina, Cleome simplicifolia and Cleome aspera species in existing barcode data. Corynandra chelidonii and Corynandra felina species belong to the Corynandra genus, but they are not grouped with the Corynandra viscosa species, however clustered with the Cleome species. Molecular marker analysis showed 100% polymorphism among the studied plant samples. Diversity indices for molecular markers were ranged from He=0.1115-0.1714 and I=0.2268-0.2700, which indicates a significant amount of genetic diversity among studied species. Discrimination of the Cleome and Corynandra species from Cleoserrata speciosa was obtained by two RAPD primers (OPA-4 and RAPD-17) and two ISSR primers (ISSR-1 and ISSR-2). RAPD and ISSR markers are useful for the genetic characterization of these studied species. The present investigation will be helpful to understand the relationships of Cleome lineages with Corynandra and Cleoserrata species.
authors.affiliation: Department of Biochemistry, Shivaji University, Kolhapur 416004, India.
authors.forename: Asif Shabodin
authors.initials: AS
authors.lastname: Tamboli
authors.affiliation: Department of Biotechnology, Shivaji University, Kolhapur 416004, India.
authors.forename: Swapnil Mahadeo
authors.initials: SM
authors.lastname: Patil
authors.affiliation: Department of Botany, Shivaji University, Kolhapur 416004, India.
authors.forename: Avinash Ramchandra
authors.initials: AR
authors.lastname: Gholave
authors.affiliation: Department of Biochemistry, Shivaji University, Kolhapur 416004, India.
authors.forename: Suhas Kishor
authors.initials: SK
authors.lastname: Kadam
authors.affiliation: Department of Biochemistry, Shivaji University, Kolhapur 416004, India.
authors.forename: Shreya Vijaykumar
authors.initials: SV
authors.lastname: Kotibhaskar
authors.affiliation: Department of Botany, Shivaji University, Kolhapur 416004, India.
authors.forename: Shrirang Ramchandra
authors.initials: SR
authors.lastname: Yadav
authors.affiliation: Department of Biochemistry, Shivaji University, Kolhapur 416004, India. Electronic address: spgovindwar@rediffmail.com.
authors.forename: Sanjay Prabhu
authors.initials: SP
authors.lastname: Govindwar
mesh_terms: D031485:Cleome / Q000145:classification / Q000235:genetics*; D058893:DNA Barcoding, Taxonomic; D018744:DNA, Plant; D005819:Genetic Markers; D014644:Genetic Variation; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers
keywords: Cleome; Cleoserrata; Corynandra; DNA barcoding; Molecular markers