title: In situ mineralization of anticancer drug into calcium carbonate monodisperse nanospheres and their pH-responsive release property.
abstract: In this paper, we facilitated the preparation of uniform calcium carbonate nanospheres and the encapsulation of anticancer drug (Doxorubicin, Dox) in one step by a facile bio-inspired mineralization method at room temperature. Hesperidin (Hesp), a natural originated flavanone glycoside, was introduced as crystallization modifier. The obtained Dox encapsulated CaCO3 nanospheres (Dox@CaCO3-Hesp NSs) having a narrow size range of ~200 nm. The drug loading/release studies reveal that these Dox@CaCO3-Hesp NSs have a drug loading efficiency (DLE) of 83% and drug loading content (DLC) of 14wt%. Besides, the release of Dox from Dox@CaCO3-Hesp NSs was pH depended. At pH=7.4, only a small amount (~28%) of Dox was released. While at pH=5.0, all amount of incorporated Dox was released. Confocal laser scanning microscopy (CLSM) image reveals the Dox@CaCO3-Hesp NSs can internalize the cells. These results suggest the Dox@CaCO3-Hesp NSs can be potentially used to utilize pH-responsive delivery of anticancer drugs.
authors.affiliation: Liaoning Medical University, Jinzhou 121001, People's Republic of China.
authors.forename: Tiezhu
authors.initials: T
authors.lastname: Yang
authors.affiliation: Liaoning Medical University, Jinzhou 121001, People's Republic of China.
authors.forename: Zhanghui
authors.initials: Z
authors.lastname: Wan
authors.affiliation: Liaoning Medical University, Jinzhou 121001, People's Republic of China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Liaoning Medical University, Jinzhou 121001, People's Republic of China.
authors.forename: Haihong
authors.initials: H
authors.lastname: Li
authors.affiliation: Liaoning Medical University, Jinzhou 121001, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Liaoning Medical University, Jinzhou 121001, People's Republic of China.
authors.forename: Nan
authors.initials: N
authors.lastname: Lu
authors.affiliation: Liaoning Medical University, Jinzhou 121001, People's Republic of China. Electronic address: zhchen561@yahoo.com.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Liaoning Medical University, Jinzhou 121001, People's Republic of China. Electronic address: meixifan1971@163.com.
authors.forename: Xifan
authors.initials: X
authors.lastname: Mei
authors.affiliation: Liaoning Medical University, Jinzhou 121001, People's Republic of China. Electronic address: rxlrenxiuli@163.com.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Ren
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000378:metabolism; D002119:Calcium Carbonate / Q000737:chemistry*; D004317:Doxorubicin / Q000737:chemistry / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D006569:Hesperidin / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D054118:Nanospheres / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D004317:Doxorubicin; D006569:Hesperidin; D002119:Calcium Carbonate
keywords: CaCO(3); Doxorubicin; Drug delivery; Nanospheres; pH-responsive