title: Steering microtubule shuttle transport with dynamically controlled magnetic fields.
abstract: Nanoscale control of matter is critical to the design of integrated nanosystems. Here, we describe a method to dynamically control directionality of microtubule (MT) motion using programmable magnetic fields. MTs are combined with magnetic quantum dots (i.e., MagDots) that are manipulated by external magnetic fields provided by magnetic nanowires. MT shuttles thus undergo both ATP-driven and externally-directed motion with a fluorescence component that permits simultaneous visualization of shuttle motion. This technology is used to alter the trajectory of MTs in motion and to pin MT motion. Such an approach could be used to evaluate the MT-kinesin transport system and could serve as the basis for improved lab-on-a-chip technologies based on MT transport.
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, 151 West Woodruff Avenue and The Ohio State University, Columbus, OH 43210, USA.
authors.forename: K D
authors.initials: KD
authors.lastname: Mahajan
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, 151 West Woodruff Avenue and The Ohio State University, Columbus, OH 43210, USA and Department of Biomedical Engineering, College of Engineering and Applied Sciences, Nanjing University, Nanjing, 200697, China.
authors.forename: G
authors.initials: G
authors.lastname: Ruan
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, 151 West Woodruff Avenue and The Ohio State University, Columbus, OH 43210, USA.
authors.forename: C J
authors.initials: CJ
authors.lastname: Dorcéna
authors.affiliation: Department of Physics, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: G
authors.initials: G
authors.lastname: Vieira
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, 151 West Woodruff Avenue and The Ohio State University, Columbus, OH 43210, USA.
authors.forename: G
authors.initials: G
authors.lastname: Nabar
authors.affiliation: Center for Integrated Nanotechnologies, Sandia National Laboratories, Albuquerque, NM 87185, USA.
authors.forename: N F
authors.initials: NF
authors.lastname: Bouxsein
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, 151 West Woodruff Avenue and The Ohio State University, Columbus, OH 43210, USA.
authors.forename: J J
authors.initials: JJ
authors.lastname: Chalmers
authors.affiliation: Center for Integrated Nanotechnologies, Sandia National Laboratories, Albuquerque, NM 87185, USA.
authors.forename: G D
authors.initials: GD
authors.lastname: Bachand
authors.affiliation: Department of Physics, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: R
authors.initials: R
authors.lastname: Sooryakumar
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, 151 West Woodruff Avenue and The Ohio State University, Columbus, OH 43210, USA and Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA. winter.63@osu.edu.
authors.forename: J O
authors.initials: JO
authors.lastname: Winter