title: Specific expression and function of inositol 1,4,5-trisphosphate 3-kinase C (ITPKC) in wild type and knock-out mice.
abstract: Inositol 1,4,5-trisphosphate 3-kinase C (ITPKC) is the last identified member of the inositol 1,4,5-trisphosphate 3-kinases family which phosphorylates inositol 1,4,5-trisphosphate into inositol 1,3,4,5-tetrakisphosphate. Although expression and function of the two other family members ITPKA and ITPKB are rather well characterized, similar information is lacking for ITPKC. Here, we first defined the expression of Itpkc mRNA and protein in mouse tissues and cells using in situ hybridization and new antibodies. Surprisingly, we found that cells positive for ITPKC in the studied tissues express either a multicilium (tracheal and bronchial epithelia, brain ependymal cells), microvilli forming a brush border (small and large intestine, and kidney proximal tubule cells) or a flagellum (spermatozoa), suggesting a role for ITPKC either in the development or the function of these specialized cellular structures. Given this surprising expression, we then analyzed ITPKC function in multiciliated tracheal epithelial cells and sperm cells using our Itpkc knock-out mouse model. Unfortunately, no significant difference was observed between control and mutant mice for any of the parameters tested, leaving the exact in vivo function of this third Ins(1,4,5)P3 3-kinase still open.
authors.affiliation: Laboratoire de Génétique Fonctionnelle, GIGA-B34, Université de Liège, avenue de l'Hôpital 11, 4000 Liège, Belgium.
authors.forename: Ariane
authors.initials: A
authors.lastname: Scoumanne
authors.affiliation: Laboratoire de Génétique Fonctionnelle, GIGA-B34, Université de Liège, avenue de l'Hôpital 11, 4000 Liège, Belgium.
authors.forename: Patricia
authors.initials: P
authors.lastname: Molina-Ortiz
authors.affiliation: Laboratoire de Parasitologie Moléculaire, Institut de Biologie et de Médecine Moléculaires (IBMM), Université Libre de Bruxelles, rue des Professeurs Jeener et Brachet 12, 6041 Gosselies, Belgium.
authors.forename: Daniel
authors.initials: D
authors.lastname: Monteyne
authors.affiliation: Laboratoire de Parasitologie Moléculaire, Institut de Biologie et de Médecine Moléculaires (IBMM), Université Libre de Bruxelles, rue des Professeurs Jeener et Brachet 12, 6041 Gosselies, Belgium; Center for Microscopy and Molecular Imaging (CMMI), Université Libre de Bruxelles (ULB), 8 rue Adrienne Bolland, B-6041 Gosselies, Belgium.
authors.forename: David
authors.initials: D
authors.lastname: Perez-Morga
authors.affiliation: Institut de Recherches Interdisciplinaires en Biologie Humaine et Moléculaire (IRIBHM), Campus Erasme, Université Libre de Bruxelles, route de Lennik, 808, 1070 Bruxelles, Belgium.
authors.forename: Christophe
authors.initials: C
authors.lastname: Erneux
authors.affiliation: Laboratoire de Génétique Fonctionnelle, GIGA-B34, Université de Liège, avenue de l'Hôpital 11, 4000 Liège, Belgium. Electronic address: sschurmans@ulg.ac.be.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Schurmans
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000201:enzymology; D002923:Cilia / Q000201:enzymology* / Q000648:ultrastructure; D004847:Epithelial Cells / Q000166:cytology / Q000201:enzymology*; D015870:Gene Expression; D017403:In Situ Hybridization; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D007295:Inositol Phosphates / Q000378:metabolism; D007420:Intestine, Large / Q000201:enzymology; D007421:Intestine, Small / Q000201:enzymology; D007687:Kidney Tubules, Proximal / Q000201:enzymology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D010766:Phosphorylation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics* / Q000378:metabolism; D061251:Primary Cell Culture; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D020545:Respiratory Mucosa / Q000166:cytology / Q000201:enzymology*; D013094:Spermatozoa / Q000201:enzymology
chemical_list: D007295:Inositol Phosphates; D012333:RNA, Messenger; C081655:inositol-1,3,4,5-tetrakisphosphate; D015544:Inositol 1,4,5-Trisphosphate; D017853:Phosphotransferases (Alcohol Group Acceptor); C051038:Inositol 1,4,5-trisphosphate 3-kinase
keywords: Inositol 1,3,4,5-tetrakisphosphate; Inositol 1,4,5-trisphosphate 3-kinase; Itpkc; Mouse; Tissue expression