title: Stable isotope probing with18O-water to investigate microbial growth and death in environmental samples.
abstract: Growth and mortality of microorganisms have been characterized through DNA stable isotope probing (SIP) with18O-water in soils from a range of ecosystems. Conventional SIP has been improved by sequencing a marker gene in all fractions retrieved from an ultracentrifuge tube to produce taxon density curves, which allow estimating the atom percent isotope composition of each microbial taxon's genome. Very recent advances in SIP with18O-water include expansion of the technique to aquatic samples, investigations of microbial turnover in soil, and the first use of18O-water in RNA-SIP studies.
authors.affiliation: Northern Arizona University, Flagstaff, AZ 86011-5640, United States. Electronic address: Egbert.Schwartz@nau.edu.
authors.forename: Egbert
authors.initials: E
authors.lastname: Schwartz
authors.affiliation: Northern Arizona University, Flagstaff, AZ 86011-5640, United States.
authors.forename: Michaela
authors.initials: M
authors.lastname: Hayer
authors.affiliation: Northern Arizona University, Flagstaff, AZ 86011-5640, United States.
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Hungate
authors.affiliation: Northern Arizona University, Flagstaff, AZ 86011-5640, United States.
authors.forename: Benjamin J
authors.initials: BJ
authors.lastname: Koch
authors.affiliation: Northern Arizona University, Flagstaff, AZ 86011-5640, United States.
authors.forename: Theresa A
authors.initials: TA
authors.lastname: McHugh
authors.affiliation: Northern Arizona University, Flagstaff, AZ 86011-5640, United States.
authors.forename: William
authors.initials: W
authors.lastname: Mercurio
authors.affiliation: Northern Arizona University, Flagstaff, AZ 86011-5640, United States.
authors.forename: Ember M
authors.initials: EM
authors.lastname: Morrissey
authors.affiliation: Northern Arizona University, Flagstaff, AZ 86011-5640, United States.
authors.forename: Katerina
authors.initials: K
authors.lastname: Soldanova
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D004783:Environmental Microbiology; D007553:Isotope Labeling / Q000379:methods*; D010103:Oxygen Isotopes / Q000032:analysis*; D014867:Water / Q000737:chemistry*
chemical_list: D004269:DNA, Bacterial; D010103:Oxygen Isotopes; D014867:Water