title: Effects of the Selected Iminosugar Derivatives on Pseudomonas aeruginosa Biofilm Formation.
abstract: A lack of an effective way to eliminate pathogenic bacteria hidden in the biofilm is a major problem in the treatment of chronic bacterial infections. Iminosugar derivatives are potential candidates for inhibitors of enzymes taking part in the biosynthesis of exopolysaccharides, which are forming bacterial biofilm. Investigated iminosugars were studied either at an early stage of biofilm formation or later on when the mature biofilm of Pseudomonas aeruginosa was already formed. A series of diverse iminosugar structures significantly inhibited biofilm formation, whereas they showed no influence on already formed biofilm. This indicates a possible mechanism of their action based on inhibition of exopolysaccharide backbone synthesis in the early stages of biofilm formation. Moreover, iminosugar derivatives did not show significant effect on the viable bacterial numbers in both early and mature biofilm forms. Importantly, they were not cytotoxic against human Caco-2 cells in vitro, which may be to their advantage in case of their medical application in preventing P. aeruginosa biofilm formation.
authors.affiliation: 1 Department of Microbiology, Jagiellonian University Medical College , Kraków, Poland .
authors.forename: Magdalena
authors.initials: M
authors.lastname: Strus
authors.affiliation: 1 Department of Microbiology, Jagiellonian University Medical College , Kraków, Poland .
authors.forename: Diana
authors.initials: D
authors.lastname: Mikołajczyk
authors.affiliation: 1 Department of Microbiology, Jagiellonian University Medical College , Kraków, Poland .
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Machul
authors.affiliation: 1 Department of Microbiology, Jagiellonian University Medical College , Kraków, Poland .
authors.forename: Piotr B
authors.initials: PB
authors.lastname: Heczko
authors.affiliation: 2 Faculty of Chemistry, Jagiellonian University , Kraków, Poland .
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Chronowska
authors.affiliation: 2 Faculty of Chemistry, Jagiellonian University , Kraków, Poland .
authors.forename: Grażyna
authors.initials: G
authors.lastname: Stochel
authors.affiliation: 3 Institut de Chimie Organique et Analytique, UMR7311, Université d'Orléans and CNRS , Orléans, France .
authors.forename: Estelle
authors.initials: E
authors.lastname: Gallienne
authors.affiliation: 3 Institut de Chimie Organique et Analytique, UMR7311, Université d'Orléans and CNRS , Orléans, France .
authors.forename: Cyril
authors.initials: C
authors.lastname: Nicolas
authors.affiliation: 3 Institut de Chimie Organique et Analytique, UMR7311, Université d'Orléans and CNRS , Orléans, France .
authors.forename: Olivier R
authors.initials: OR
authors.lastname: Martin
authors.affiliation: 2 Faculty of Chemistry, Jagiellonian University , Kraków, Poland .
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kyzioł
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D018938:Caco-2 Cells; D002470:Cell Survival / Q000187:drug effects; D015169:Colony Count, Microbial; D006801:Humans; D050111:Imino Sugars / Q000737:chemistry / Q000494:pharmacology*; D050296:Microbial Viability / Q000187:drug effects; D011135:Polysaccharides, Bacterial / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D013045:Species Specificity; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D050111:Imino Sugars; D011135:Polysaccharides, Bacterial
keywords: Pseudomonas aeruginosa; biofilm; iminosugars