title: Effect of glutamine supplementation and replacement of tris-egg yolk based extender with defatted cow milk on spermatozoa quality after equilibration and thawing.
abstract: OBJECTIVE
  The present study was designed to evaluate the effect of supplementation of glutamine and replacement of Tris-egg yolk (TE) based buffer with defatted cow milk on the spermatozoa quality after equilibration and thawing.
  
  
  METHODS
  Semen was collected from five Bhadawari bulls biweekly, and a total of 30 ejaculates were taken. The semen ejaculates were pooled and divided into three equal parts. The pooled semen was diluted by TE based extender (control), TE + glutamine (8 mM) (T1) and 50% TE + 50% deffated cow milk + glutamine (8 mM) (T2). At two stages viz. after equilibration and after 12 h of cryopreservation (thawed samples), progressive motility, percent live, and percent acrosomal damage of the spermatozoa was assessed.
  
  
  RESULTS
  Supplementation of glutamine improved (p<0.05) the spermatozoa quality with respect to the progressive motility, percent live and acrosomal damage both post-equilibration and post-thaw. T2 improved (p<0.05) the spermatozoa quality as compared to control, however; it was less (p<0.05) effective as compared T1 both post-equilibration and post-thaw.
  
  
  CONCLUSIONS
  From the results of present study it can be concluded that glutamine supplementation was effective in maintaining post-equilibration and post-thaw spermatozoa quality whereas defatted cow milk was not as effective as TE based buffer in the extender in improving the spermatozoa quality.
authors.affiliation: Department of Veterinary Physiology, College of Veterinary Science and Animal Husbandry, Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vishwavidyalaya Evam Go Anusndhan Sansthan, Mathura, Uttar Pradesh, India.
authors.forename: Vasundhara
authors.initials: V
authors.lastname: Dawra
authors.affiliation: Department of Veterinary Physiology, College of Veterinary Science and Animal Husbandry, Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vishwavidyalaya Evam Go Anusndhan Sansthan, Mathura, Uttar Pradesh, India.
authors.forename: Brijesh
authors.initials: B
authors.lastname: Yadav
authors.affiliation: Department of Veterinary Physiology, College of Veterinary Science and Animal Husbandry, Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vishwavidyalaya Evam Go Anusndhan Sansthan, Mathura, Uttar Pradesh, India.
authors.forename: Sarvajeet
authors.initials: S
authors.lastname: Yadav
keywords: Bhadawari bull; defatted milk; glutamine; post-thaw spermatozoa quality