title: Expression of human papillomavirus E6 and E7 oncoprotein mRNA in women with low-grade squamous intraepithelial lesions or less.
abstract: We verified the prevalence of human papillomavirus (HPV) E6/E7 protein mRNA expression in patients with low-grade squamous intraepithelial lesions (LSILs) and negative cervicovaginal cytology. To investigate the relationship between mRNA expression and viral infection type, we assessed genotyping in single infections. Samples from 825 women were submitted to the E6/E7 survey. We noticed a larger percentage of E6/E7 mRNA expression in the atypical squamous cells of undetermined significance (ASC-US) and LSIL cytologies. Negative results of mRNA expression were in accordance with negative cytologies. In positive cases, the infection by a single HPV type was most common, with type 16 being most prevalent. The expression of mRNA was most prevalent in ASC-US and LSIL cytologies, compared with the negative cytology. The infection by a HPV type was more frequent in cases of positive expression, with HPV type 16 being found most frequently. Patients with LSIL cytologies had a higher percentage of multiple infections.
authors.affiliation: Núcleo de Prevenção de Doenças Ginecológicas, Departamento de Ginecologia, Escola Paulista de Medicina, São Paulo, SP, Brasil.
authors.forename: D C
authors.initials: DC
authors.lastname: Casagrande
authors.affiliation: Núcleo de Prevenção de Doenças Ginecológicas, Departamento de Ginecologia, Escola Paulista de Medicina, São Paulo, SP, Brasil.
authors.forename: J C L
authors.initials: JC
authors.lastname: Ribalta
authors.affiliation: Departamento de Patologia, Escola Paulista de Medicina,Universidade Federal de São Paulo, São Paulo, SP, Brasil.|Laboratório Salomão & Zoppi Diagnósticos, São Paulo, SP, Brasil.
authors.forename: K D P F
authors.initials: KD
authors.lastname: Leite
authors.affiliation: Núcleo de Prevenção de Doenças Ginecológicas, Departamento de Ginecologia, Escola Paulista de Medicina, São Paulo, SP, Brasil.
authors.forename: M
authors.initials: M
authors.lastname: Schimidt
authors.affiliation: Núcleo de Prevenção de Doenças Ginecológicas, Departamento de Ginecologia, Escola Paulista de Medicina, São Paulo, SP, Brasil.|Laboratório Salomão & Zoppi Diagnósticos, São Paulo, SP, Brasil.
authors.forename: N M D G
authors.initials: NM
authors.lastname: Speck
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000378:metabolism; D050725:Papillomavirus E7 Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D065310:Squamous Intraepithelial Lesions of the Cervix / Q000235:genetics* / Q000473:pathology / Q000821:virology
chemical_list: C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins