title: The Prognostic Influence of BRAF Mutation and other Molecular, Clinical and Laboratory Parameters in Stage IV Colorectal Cancer.
abstract: Our aim was to evaluate the predictive and prognostic influence of BRAF mutation and other molecular, clinical and laboratory parameters in stage IV colorectal cancer (CRC). 60 patients were included in this retrospective analysis, and 17 variables were examined for their relation with treatment response and survival. KRAS mutation was identified in 40.3 % of cases, BRAF and PIK3CA in 8.8 % and 10.5 % respectively. 29.8 % of patients responded to treatment. Median survival time was 14.3 months. Weight loss, fever, abdominal metastases, blood transfusion, hypoalbuminaimia, BRAF and PIK3CA mutations, CRP and DNA Index were associated with survival. In multivariate analysis, male patients had 3.8 times higher probability of response, increased DNA Index was inversely correlated with response and one unit raise of DNA Index augmented 6 times the probability of death. Our findings potentiate the prognostic role of BRAF, PIK3CA mutations and ploidy in advanced CRC.
authors.affiliation: Oncology Unit, Department of Pathophysiology, Medical School, National and Kapodistrian University of Athens, Laiko General Hospital, Mikras Asias 75 Street, 11527, Athens, Greece. m_karadima@hotmail.com.
authors.forename: Maria L
authors.initials: ML
authors.lastname: Karadima
authors.affiliation: First Department of Pathology, Medical School, National and Kapodistrian University of Athens, Laiko General Hospital, Athens, Greece.
authors.forename: Angelica A
authors.initials: AA
authors.lastname: Saetta
authors.affiliation: First Department of Pathology, Medical School, National and Kapodistrian University of Athens, Laiko General Hospital, Athens, Greece.
authors.forename: Ilenia
authors.initials: I
authors.lastname: Chatziandreou
authors.affiliation: First Department of Pathology, Medical School, National and Kapodistrian University of Athens, Laiko General Hospital, Athens, Greece.
authors.forename: Andreas C
authors.initials: AC
authors.lastname: Lazaris
authors.affiliation: First Department of Pathology, Medical School, National and Kapodistrian University of Athens, Laiko General Hospital, Athens, Greece.
authors.forename: Efstratios
authors.initials: E
authors.lastname: Patsouris
authors.affiliation: Oncology Unit, Department of Pathophysiology, Medical School, National and Kapodistrian University of Athens, Laiko General Hospital, Mikras Asias 75 Street, 11527, Athens, Greece.
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Tsavaris
mesh_terms: D058534:Class I Phosphatidylinositol 3-Kinases; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics*; D009367:Neoplasm Staging; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics; D011379:Prognosis; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics; D012189:Retrospective Studies
chemical_list: C117307:KRAS protein, human; D019869:Phosphatidylinositol 3-Kinases; D058534:Class I Phosphatidylinositol 3-Kinases; C484760:PIK3CA protein, human; C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf; D016283:Proto-Oncogene Proteins p21(ras)
keywords: BRAF; Colorectal cancer; DNA ploidy; KRAS; PIK3CA; Prognosis