title: Long-Term Fructose Intake Increases Adipogenic Potential: Evidence of Direct Effects of Fructose on Adipocyte Precursor Cells.
abstract: We have previously addressed that fructose rich diet (FRD) intake for three weeks increases the adipogenic potential of stromal vascular fraction cells from the retroperitoneal adipose tissue (RPAT). We have now evaluated the effect of prolonged FRD intake (eight weeks) on metabolic parameters, number of adipocyte precursor cells (APCs) and in vitro adipogenic potential from control (CTR) and FRD adult male rats. Additionally, we have examined the direct fructose effects on the adipogenic capacity of normal APCs. FRD fed rats had increased plasma levels of insulin, triglyceride and leptin, and RPAT mass and adipocyte size. FACS studies showed higher APCs number and adipogenic potential in FRD RPAT pads; data is supported by high mRNA levels of competency markers: PPARγ2 and Zfp423. Complementary in vitro experiments indicate that fructose-exposed normal APCs displayed an overall increased adipogenic capacity. We conclude that the RPAT mass expansion observed in eight week-FRD fed rats depends on combined accelerated adipogenesis and adipocyte hypertrophy, partially due to a direct effect of fructose on APCs.
authors.affiliation: Neuroendocrinology Laboratory, Multidisciplinary Institute of Cellular Biology (IMBICE, CICPBA-CONICET-UNLP), Calles 526 10 y 11, La Plata 1900, Argentina. gzubiria@imbice.gov.ar.|Biology Department, School of Exact Sciences, Universidad Nacional de La Plata, La Plata 1900, Argentina. gzubiria@imbice.gov.ar.
authors.forename: María Guillermina
authors.initials: MG
authors.lastname: Zubiría
authors.affiliation: Neuroendocrinology Laboratory, Multidisciplinary Institute of Cellular Biology (IMBICE, CICPBA-CONICET-UNLP), Calles 526 10 y 11, La Plata 1900, Argentina. aalzamendi@imbice.gov.ar.
authors.forename: Ana
authors.initials: A
authors.lastname: Alzamendi
authors.affiliation: Institute of Immunological and Physiopathological Research (IIFP, CONICET-UNLP), School of Exact Sciences, Universidad Nacional de La Plata, La Plata 1900, Argentina. gmoreno@iifp.laplata-conicet.gov.ar.
authors.forename: Griselda
authors.initials: G
authors.lastname: Moreno
authors.affiliation: Neuroendocrinology Laboratory, Multidisciplinary Institute of Cellular Biology (IMBICE, CICPBA-CONICET-UNLP), Calles 526 10 y 11, La Plata 1900, Argentina. mamandarey@gmail.com.
authors.forename: María Amanda
authors.initials: MA
authors.lastname: Rey
authors.affiliation: Center of Experimental and Applied Endocrinology (CENEXA, UNLP-CONICET, PAHO/WHO Collaborating Center for Diabetes), La Plata Medical School, Universidad Nacional de La Plata, La Plata 1900, Argentina. spinedi@cenexa.org.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Spinedi
authors.affiliation: Neuroendocrinology Laboratory, Multidisciplinary Institute of Cellular Biology (IMBICE, CICPBA-CONICET-UNLP), Calles 526 10 y 11, La Plata 1900, Argentina. agiovamba@imbice.gov.ar.|Biology Department, School of Exact Sciences, Universidad Nacional de La Plata, La Plata 1900, Argentina. agiovamba@imbice.gov.ar.
authors.forename: Andrés
authors.initials: A
authors.lastname: Giovambattista
mesh_terms: D017667:Adipocytes / Q000187:drug effects*; D050156:Adipogenesis / Q000187:drug effects; D000818:Animals; D001835:Body Weight; D004334:Drug Administration Schedule; D002149:Energy Intake; D005632:Fructose / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005632:Fructose
keywords: SVF cells; adipogenesis; precursor cell competency; retroperitoneal adipose tissue