title: Thrombospondin Type-1 Repeat Domain-Containing Proteins Are Strongly Expressed in the Head Region of Hydra.
abstract: The head region of Hydra, the hypostome, is a key body part for developmental control and the nervous system. We herein examined genes specifically expressed in the head region of Hydra oligactis using suppression subtractive hybridization (SSH) cloning. A total of 1414 subtracted clones were sequenced and found to be derived from at least 540 different genes by BLASTN analyses. Approximately 25% of the subtracted clones had sequences encoding thrombospondin type-1 repeat (TSR) domains, and were derived from 17 genes. We identified 11 TSR domain-containing genes among the top 36 genes that were the most frequently detected in our SSH library. Whole-mount in situ hybridization analyses confirmed that at least 13 out of 17 TSR domain-containing genes were expressed in the hypostome of Hydra oligactis. The prominent expression of TSR domain-containing genes suggests that these genes play significant roles in the hypostome of Hydra oligactis.
authors.affiliation: Department of Food and Health Sciences, International College of Arts and Sciences, Fukuoka Women's University, Fukuoka, Japan.
authors.forename: Kayoko
authors.initials: K
authors.lastname: Hamaguchi-Hamada
authors.affiliation: Department of Food and Health Sciences, International College of Arts and Sciences, Fukuoka Women's University, Fukuoka, Japan.
authors.forename: Mami
authors.initials: M
authors.lastname: Kurumata-Shigeto
authors.affiliation: Department of Environmental Sciences, International College of Arts and Sciences, Fukuoka Women's University, Fukuoka, Japan.
authors.forename: Sumiko
authors.initials: S
authors.lastname: Minobe
authors.affiliation: Department of Environmental Sciences, International College of Arts and Sciences, Fukuoka Women's University, Fukuoka, Japan.
authors.forename: Nozomi
authors.initials: N
authors.lastname: Fukuoka
authors.affiliation: Department of Environmental Sciences, International College of Arts and Sciences, Fukuoka Women's University, Fukuoka, Japan.
authors.forename: Manami
authors.initials: M
authors.lastname: Sato
authors.affiliation: Department of Food and Health Sciences, International College of Arts and Sciences, Fukuoka Women's University, Fukuoka, Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Matsufuji
authors.affiliation: Department of Environmental Sciences, International College of Arts and Sciences, Fukuoka Women's University, Fukuoka, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Koizumi
authors.affiliation: Department of Food and Health Sciences, International College of Arts and Sciences, Fukuoka Women's University, Fukuoka, Japan.
authors.forename: Shun
authors.initials: S
authors.lastname: Hamada
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation / Q000502:physiology*; D006829:Hydra / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D020449:Repetitive Sequences, Amino Acid; D020539:Sequence Analysis, Protein