title: [The Clinical Signs of Experimental Autoimmune Uveitis].
abstract: BACKGROUND
  Autoimmune uveitis is a sight threatening disease which in many cases fails to respond to conventional immunosuppressive or biological therapy. The research in experimental models of autoimmune uveitis helps to find new therapeutical strategies. The aim of this study is to present the clinical and histological signs of experimental autoimmune uveitis (EAU) in mice.
  
  
  METHODS
  EAU was induced in C57BL/6 mice by subcutaneous application of IRBP (interphotoreceptor retinoid binding protein) in complete Freunds adjuvant and intraperitoneal application of pertussis toxin. Clinical evaluation of uveitis was performed in vivo using special imaging system with otoscope. Histological evaluation of uveitis was performed at day 35 post induction of EAU on hematoxylin and eosin stained frozen sections. Clinical and histological grading was used to assess the inflammation intensity of EAU.
  
  
  RESULTS
  The intensity of inflammation is depicted on representative fundus images and histological images of retina at day 35 post induction.
  
  
  CONCLUSIONS
  The model of EAU is robust and reproducible and allows us to study the immunopathological mechanisms of inflammation and its regulation. The inflammatory signs in our model are similar to findings of posterior uveitis of autoimmune etiology in humans, thus we may apply our experimental results in human medicine.
authors.forename: A
authors.initials: A
authors.lastname: Klímová
authors.forename: P
authors.initials: P
authors.lastname: Seidler Štangová
authors.forename: P
authors.initials: P
authors.lastname: Svozílková
authors.forename: T
authors.initials: T
authors.lastname: Kučera
authors.forename: J
authors.initials: J
authors.lastname: Heissigerová
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000139:chemically induced / Q000175:diagnosis*; D004195:Disease Models, Animal; D005136:Eye Proteins / Q000633:toxicity; D005654:Fundus Oculi; D007166:Immunosuppressive Agents; D008810:Mice, Inbred C57BL; D012160:Retina / Q000473:pathology*; D012177:Retinol-Binding Proteins / Q000633:toxicity; D014605:Uveitis / Q000139:chemically induced / Q000175:diagnosis*; D000099214:Retinol-Binding Proteins, Interstitial
chemical_list: D005136:Eye Proteins; D007166:Immunosuppressive Agents; D012177:Retinol-Binding Proteins; D000099214:Retinol-Binding Proteins, Interstitial