title: A comprehensive strategy for the analysis of acoustic compressibility and optical deformability on single cells.
abstract: We realized an integrated microfluidic chip that allows measuring both optical deformability and acoustic compressibility on single cells, by optical stretching and acoustophoresis experiments respectively. Additionally, we propose a measurement protocol that allows evaluating the experimental apparatus parameters before performing the cell-characterization experiments, including a non-destructive method to characterize the optical force distribution inside the microchannel. The chip was used to study important cell-mechanics parameters in two human breast cancer cell lines, MCF7 and MDA-MB231. Results indicate that MDA-MB231 has both higher acoustic compressibility and higher optical deformability than MCF7, but statistical analysis shows that optical deformability and acoustic compressibility are not correlated parameters. This result suggests the possibility to use them to analyze the response of different cellular structures. We also demonstrate that it is possible to perform both measurements on a single cell, and that the order of the two experiments does not affect the retrieved values.
authors.affiliation: Department of Electrical, Computer, and Biomedical Engineering, Università di Pavia, Via Ferrata 5A, 27100, Pavia, Italy.
authors.forename: Tie
authors.initials: T
authors.lastname: Yang
authors.affiliation: Institute of Photonics and Nanotechnology, CNR &Department of Physics, Politecnico di Milano, Piazza, Leonardo da Vinci 32, 20133 Milano, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Bragheri
authors.affiliation: Department of Biomedical Science and Translational Medicine, Università di Milano, Via F.lli Cervi 91, 20090 Segrate, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Nava
authors.affiliation: Institute of Molecular Genetics (IGM), CNR, Via Abbiategrasso 207, 27100 Pavia, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Chiodi
authors.affiliation: Institute of Molecular Genetics (IGM), CNR, Via Abbiategrasso 207, 27100 Pavia, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Mondello
authors.affiliation: Institute of Photonics and Nanotechnology, CNR &Department of Physics, Politecnico di Milano, Piazza, Leonardo da Vinci 32, 20133 Milano, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Osellame
authors.affiliation: Department of Physics, Technical University of Denmark, DK-2800 Kgs, Lyngby, Denmark.
authors.forename: Kirstine
authors.initials: K
authors.lastname: Berg-Sørensen
authors.affiliation: Department of Electrical, Computer, and Biomedical Engineering, Università di Pavia, Via Ferrata 5A, 27100, Pavia, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Cristiani
authors.affiliation: Department of Electrical, Computer, and Biomedical Engineering, Università di Pavia, Via Ferrata 5A, 27100, Pavia, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Minzioni
mesh_terms: D000162:Acoustics / Q000295:instrumentation*; D045744:Cell Line, Tumor; D006801:Humans; D056656:Lab-On-A-Chip Devices; D061986:MCF-7 Cells; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D055620:Optical Phenomena; D059010:Single-Cell Analysis / Q000295:instrumentation / Q000379:methods*