title: The fluoride coated AZ31B magnesium alloy improves corrosion resistance and stimulates bone formation in rabbit model.
abstract: This study aimed to evaluate the effect of fluorine coated Mg alloy and clarify its mechanism in bone formation. We implanted the fluorine coated AZ31B Mg alloy screw (group F) in rabbit mandibular and femur in vivo. Untreated AZ31B Mg alloy screw (group A) and titanium screw (group T) were used as control. Then, scanning electron microscopy, the spectral energy distribution analysis, hard and decalcified bone tissues staining were performed. Immunohistochemistry was employed to examine the protein expressions of bone morphogenetic protein 2 (BMP-2) and collagen type I in the vicinity of the implant. Compared with the group A, the degradation of the alloy was reduced, the rates of Mg corrosion and Mg ion release were slowed down, and the depositions of calcium and phosphate increased in the group F in the early stage of implantation. Histological results showed that fluorine coated Mg alloy had well osteogenic activity and biocompatibility. Moreover, fluoride coating obviously up-regulated the expressions of collagen type I and BMP-2. This study confirmed that the fluorine coating might improve the corrosion resistance of AZ31B Mg alloy and promote bone formation by up-regulated the expressions of collagen type I and BMP-2.
authors.affiliation: Department of Prosthodontics, School of Stomatology, China Medical University, Shenyang 110001, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.affiliation: Department of Prosthodontics, School of Stomatology, China Medical University, Shenyang 110001, People's Republic of China.
authors.forename: Guangdao
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, People's Republic of China.
authors.forename: Lili
authors.initials: L
authors.lastname: Tan
authors.affiliation: Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, People's Republic of China.
authors.forename: Ke
authors.initials: K
authors.lastname: Yang
authors.affiliation: Department of Prosthodontics, School of Stomatology, China Medical University, Shenyang 110001, People's Republic of China. Electronic address: aihongjuna@sina.com.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Ai
mesh_terms: D000497:Alloys / Q000737:chemistry*; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D001863:Bone Screws; D001842:Bone and Bones / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology; D024042:Collagen Type I / Q000378:metabolism; D003343:Corrosion; D004195:Disease Models, Animal; D005269:Femur / Q000293:injuries / Q000473:pathology / Q000502:physiology; D005459:Fluorides / Q000737:chemistry*; D008274:Magnesium / Q000737:chemistry*; D008297:Male; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D010012:Osteogenesis / Q000187:drug effects; D011817:Rabbits
chemical_list: D000497:Alloys; D055396:Bone Morphogenetic Protein 2; D020099:Coated Materials, Biocompatible; D024042:Collagen Type I; D008274:Magnesium; D005459:Fluorides
keywords: Bone morphogenetic protein 2; Collagen type I; Corrosion resistance; Fluorine coated AZ31B magnesium alloy; Osteogenic activity