title: The isolation and identification of new microalgal strains producing oil and carotenoid simultaneously with biofuel potential.
abstract: Taxonomy and phylogeny of twenty two microalgal isolates were examined using both universal and newly designed molecular primers. Among the isolates, Scenedesmus bijugus, Coelastrella sp., Auxenochlorella protothecoides, and Chlorella sp. were particularly promising in terms of producing lipids as measured by fatty acid methyl esters (FAME) analysis and significant concentration of carotenoids. A comparative experiment showed that S. bijugus and Chlorella sp. were the most promising candidates (L(-)(1)d(-)(1), with biomass) 174.77±6.75, 169.81±5.22mg, lipids 40.14±3.31, 39.72±3.89mg, lutein 0.47, 0.36mg, and astaxanthin 0.27, 0.18mg respectively. The fatty acids produced by these microalgal isolates were mainly palmitic, stearic, oleic, linoleic, and linolenic acid. The freshwater microalgal isolate S. bijugus be the most suitable isolate for producing biodiesel and carotenoids, due to high productivity of biomass, lipids, metabolites, and its suitable fatty acid profile.
authors.affiliation: TERI-Deakin Nanobiotechnology Centre, Biotechnology and Management of Bioresources Division, The Energy and Resources Institute, New Delhi, India; School of Life and Environmental Sciences, Deakin University, Geelong campus at Waurn Ponds, Victoria 3217, Australia.
authors.forename: Amritpreet Kaur
authors.initials: AK
authors.lastname: Minhas
authors.affiliation: Institute for Frontier Materials, Deakin University, Geelong campus at Waurn Ponds, Victoria 3217, Australia.
authors.forename: Peter
authors.initials: P
authors.lastname: Hodgson
authors.affiliation: School of Life and Environmental Sciences, Deakin University, Geelong campus at Waurn Ponds, Victoria 3217, Australia.
authors.forename: Colin J
authors.initials: CJ
authors.lastname: Barrow
authors.affiliation: TERI-Deakin Nanobiotechnology Centre, Biotechnology and Management of Bioresources Division, The Energy and Resources Institute, New Delhi, India.
authors.forename: Burla
authors.initials: B
authors.lastname: Sashidhar
authors.affiliation: TERI-Deakin Nanobiotechnology Centre, Biotechnology and Management of Bioresources Division, The Energy and Resources Institute, New Delhi, India. Electronic address: aloka@teri.res.in.
authors.forename: Alok
authors.initials: A
authors.lastname: Adholeya
mesh_terms: D001483:Base Sequence; D056804:Biofuels; D018533:Biomass; D002338:Carotenoids / Q000096:biosynthesis*; D002708:Chlorella / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis / Q000737:chemistry; D058086:Microalgae / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D008055:Lipids; D002338:Carotenoids
keywords: Biofuel; Bioprospecting; Carotenoids; Lipids; Microalgae