title: A Candida guilliermondii lysine hyperproducer capable of elevated citric acid production.
abstract: A mutant of the yeast Candida guilliermondii ATCC 9058 exhibiting elevated citric acid production was isolated based upon its ability to overproduce lysine. This method involved the use of a solid medium containing a combination of lysine analogues to identify a mutant that produced a several-fold higher lysine level compared to its parent strain using glucose or glycerol as a carbon source. The mutant strain was also capable of producing more than a fivefold higher citric acid level on glycerol as a carbon source compared to its parent strain. It was concluded that the screening of yeast lysine hyperproducer strains could provide a rapid approach to isolate yeast citric acid hyperproducer strains.
authors.affiliation: Department of Biology and Microbiology, South Dakota State University, Brookings, SD, 57007, USA. Thomas.West@tamuc.edu.|Department of Chemistry, Texas A&M University-Commerce, Commerce, TX, 75429, USA. Thomas.West@tamuc.edu.
authors.forename: Thomas P
authors.initials: TP
authors.lastname: West
mesh_terms: D002175:Candida / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D019343:Citric Acid / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D007218:Industrial Microbiology; D008239:Lysine / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D019343:Citric Acid; D005947:Glucose; D008239:Lysine; D005990:Glycerol
keywords: Analogue; Candida; Citric acid; Lysine; Mutant