title: Insufficient disease inhibition by intrathecal rituximab in progressive multiple sclerosis.
abstract: Inaccessibility of the inflammation compartmentalized to the central nervous system (CNS) may underlie the lack of efficacy of immunomodulatory treatments in progressive multiple sclerosis (MS). The double blind combination of Rituximab by IntraVenous and IntraThecAl injection versus placebo in patients with Low-Inflammatory SEcondary progressive MS (RIVITALISE; NCT01212094) trial was designed to answer: (1) Whether an induction dose of intravenous and intrathecal rituximab efficiently depletes CNS B cells? and (2) If so, whether this leads to global inhibition of CNS inflammation and slowing of CNS tissue destruction?
  
  
  
  Patients aged 18-65 years were randomly assigned to rituximab or placebo. Protocol-stipulated interim analysis quantified the efficacy of B-cell depletion.
  
  
  
  The efficacy on cerebrospinal fluid (CSF) biomarkers failed to reach criteria for continuation of the trial. B-cell-related CSF biomarkers (sCD21 and B-cell activating factor) changed only in the active-treatment arm. While CSF B cells were killed robustly (median -79.71%, P = 0.0176), B cells in CNS tissue were depleted inadequately (~-10-20%, P < 0.0001). Consequently, the T-cell-specific CSF biomarker sCD27 decreased slightly (-10.97%, P = 0.0005), while axonal damage marker, neurofilament light chain did not change. Insufficient saturation of CD20, lack of lytic complement, and paucity of cytotoxic CD56(dim) NK cells contribute to decreased efficacy of rituximab in the CNS.
  
  
  
  Biomarker studies reliably quantified complementary pharmacodynamic effects of rituximab in the CNS, exposed causes for poor efficacy and determined that RIVITALISE trial would be underpowered to measure efficacy on clinical outcomes. Identified mechanisms for poor efficacy are applicable to all CNS-inflammation targeting monoclonal antibodies.
authors.affiliation: Neuroimmunological Diseases Unit National Institute of Neurological Disorders and Stroke (NINDS) Bethesda Maryland.
authors.forename: Mika
authors.initials: M
authors.lastname: Komori
authors.affiliation: Neuroimmunological Diseases Unit National Institute of Neurological Disorders and Stroke (NINDS) Bethesda Maryland.
authors.forename: Yen Chih
authors.initials: YC
authors.lastname: Lin
authors.affiliation: Neuroimmunology Clinic NINDS Bethesda Maryland.
authors.forename: Irene
authors.initials: I
authors.lastname: Cortese
authors.affiliation: Neuroimmunological Diseases Unit National Institute of Neurological Disorders and Stroke (NINDS) Bethesda Maryland.
authors.forename: Andrew
authors.initials: A
authors.lastname: Blake
authors.affiliation: Neuroimmunology Clinic NINDS Bethesda Maryland.
authors.forename: Joan
authors.initials: J
authors.lastname: Ohayon
authors.affiliation: Neuroimmunological Diseases Unit National Institute of Neurological Disorders and Stroke (NINDS) Bethesda Maryland.
authors.forename: Jamie
authors.initials: J
authors.lastname: Cherup
authors.affiliation: Flow Cytometry Core Facility NINDS Bethesda Maryland.
authors.forename: Dragan
authors.initials: D
authors.lastname: Maric
authors.affiliation: Neuroimmunological Diseases Unit National Institute of Neurological Disorders and Stroke (NINDS) Bethesda Maryland.
authors.forename: Peter
authors.initials: P
authors.lastname: Kosa
authors.affiliation: Clinical Neuroscience Program NINDS Bethesda Maryland.
authors.forename: Tianxia
authors.initials: T
authors.lastname: Wu
authors.affiliation: Neuroimmunological Diseases Unit National Institute of Neurological Disorders and Stroke (NINDS) Bethesda Maryland; NIH Center for Human Immunology (CHI)the National Institutes of Health (NIH) Bethesda Maryland.
authors.forename: Bibiana
authors.initials: B
authors.lastname: Bielekova