title: Molecular Detection and Phylogenetic Characterization of Bat and Human Adenoviruses in Southern China.
abstract: Several novel adenoviruses (AdVs) have recently been identified in humans and other animal species. In this study, we report the molecular detection of and phylogenetically characterize bat and human AdVs detected in fecal or rectal swab samples collected in southern China. To detect AdVs, a 252-261 bp fragment of the DNA polymerase (DPOL) gene was amplified using nested PCR. A total of 520 rectal swab samples were collected from eight bat species in four geographic regions of southern China (Guangzhou, Yunfu, Huizhou, and Haikou city). Thirty-six (6.9%) samples from the following species tested positive for AdVs: Myotis ricketti, Miniopterus schreibersii, Scotophilus kuhlii, Taphozous melanopogon, Rhinolophus blythi, and Cynopterus sphinx. Eight novel AdVs were detected in 13.3% of the samples from C. sphinx. Of 328 fecal samples from patients with diarrhea, 16 (4.9%) were positive for classical human AdVs. Phylogenetic analysis showed that human AdVs shared low similarity (57.1-69.3%) with bat AdVs in deduced amino acid sequences of the AdV DPOL region. Thus, our study indicated that bat AdVs and human AdVs are species specific. As such, there is no evidence of cross-species transmission of AdV between bats and humans based on current data.
authors.affiliation: 1 Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University , Guangzhou, Guangdong, China .
authors.forename: Xue-Yan
authors.initials: XY
authors.lastname: Zheng
authors.affiliation: 1 Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University , Guangzhou, Guangdong, China .
authors.forename: Min
authors.initials: M
authors.lastname: Qiu
authors.affiliation: 1 Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University , Guangzhou, Guangdong, China .
authors.forename: Hui-Fang
authors.initials: HF
authors.lastname: Chen
authors.affiliation: 1 Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University , Guangzhou, Guangdong, China .
authors.forename: Shao-Wei
authors.initials: SW
authors.lastname: Chen
authors.affiliation: 1 Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University , Guangzhou, Guangdong, China .
authors.forename: Jian-Peng
authors.initials: JP
authors.lastname: Xiao
authors.affiliation: 1 Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University , Guangzhou, Guangdong, China .
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Jiang
authors.affiliation: 1 Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University , Guangzhou, Guangdong, China .
authors.forename: Shu-Ting
authors.initials: ST
authors.lastname: Huo
authors.affiliation: 1 Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University , Guangzhou, Guangdong, China .
authors.forename: Ting-Li
authors.initials: TL
authors.lastname: Shi
authors.affiliation: 1 Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University , Guangzhou, Guangdong, China .
authors.forename: Li-Zhen
authors.initials: LZ
authors.lastname: Ma
authors.affiliation: 1 Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University , Guangzhou, Guangdong, China .
authors.forename: Shan
authors.initials: S
authors.lastname: Liu
authors.affiliation: 1 Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University , Guangzhou, Guangdong, China .
authors.forename: Jun-Hua
authors.initials: JH
authors.lastname: Zhou
authors.affiliation: 1 Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University , Guangzhou, Guangdong, China .
authors.forename: Qiong-Hua
authors.initials: QH
authors.lastname: Zhang
authors.affiliation: 1 Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University , Guangzhou, Guangdong, China .
authors.forename: Xing
authors.initials: X
authors.lastname: Li
authors.affiliation: 2 College of Life Science, Hainan Normal University , Haikou, China .
authors.forename: Zhong
authors.initials: Z
authors.lastname: Chen
authors.affiliation: 3 College of Life Science, Guangzhou University , Guangzhou, China .
authors.forename: Yi
authors.initials: Y
authors.lastname: Wu
authors.affiliation: 4 Department of Bioinformatics, School of Basic Medical Sciences, Southern Medical University , Guangzhou, China .
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Li
authors.affiliation: 5 State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Disease , Guangzhou, China .
authors.forename: Wei-Jie
authors.initials: WJ
authors.lastname: Guan
authors.affiliation: 1 Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University , Guangzhou, Guangdong, China .
authors.forename: Yi-Quan
authors.initials: YQ
authors.lastname: Xiong
authors.affiliation: 1 Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University , Guangzhou, Guangdong, China .
authors.forename: Shu-Juan
authors.initials: SJ
authors.lastname: Ma
authors.affiliation: 1 Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University , Guangzhou, Guangdong, China .
authors.forename: Xue-Shan
authors.initials: XS
authors.lastname: Zhong
authors.affiliation: 1 Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University , Guangzhou, Guangdong, China .
authors.forename: Jing
authors.initials: J
authors.lastname: Ge
authors.affiliation: 1 Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University , Guangzhou, Guangdong, China .
authors.forename: Shu-Wen
authors.initials: SW
authors.lastname: Cen
authors.affiliation: 1 Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University , Guangzhou, Guangdong, China .
authors.forename: Qing
authors.initials: Q
authors.lastname: Chen
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D002681:China; D002685:Chiroptera / Q000821:virology*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics / Q000378:metabolism; D005243:Feces / Q000821:virology; D015971:Gene Expression Regulation, Enzymologic; D015967:Gene Expression Regulation, Viral; D006801:Humans; D010802:Phylogeny
chemical_list: D004259:DNA-Directed DNA Polymerase
keywords: Adenovirus; Bat; Human