title: [Preparation and characterization of a monoclonal antibody against human tissue factor with anticoagulation activity].
abstract: OBJECTIVE
  To prepare and characterize a monoclonal antibody (mAb) against human tissue factor (hTF) with anticoagulation activity.
  
  
  METHODS
  BALB/c mice were immunized with truncated recombinant protein (rhTF243). Hybridoma cell lines were generated from cell fusion, and screened using indirect ELISA and prothrombin time (PT). After ascites was developed in BALB/c mice, antibody titers were determined using indirect ELISA. Western blotting was performed to study the antibody specificity. Anticoagulant activity of the antibody was detected by PT assay.
  
  
  RESULTS
  A mAb to hTF with excellent anticoagulation activity was identified. Its immunoglobulin subclass belonged to IgG1. Titer of ascites fluid was 1:200 000. Western blotting and PT analysis confirmed the specificity and anticoagulant activity of the antibody. The mAb reacted specifically to both recombinant hTF243 and natural TF on SW620 colon cancer cell surface.
  
  
  CONCLUSIONS
  A hTF mAb with anticoagulation activity and high specificity has been successfully prepared.
authors.affiliation: School of Life Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Life Science, Shanxi University, Taiyuan 030006, China. *Corresponding authors, E-mail: xhwang@sxu.edu.cn.
authors.forename: Xinghua
authors.initials: X
authors.lastname: Wang
authors.affiliation: Room for Genetic Pharmacy, Shanxi Institute of Biology, Taiyuan 030006, China.
authors.forename: Yongji
authors.initials: Y
authors.lastname: He
authors.affiliation: Room for Genetic Pharmacy, Shanxi Institute of Biology, Taiyuan 030006, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Pan
authors.affiliation: Room for Genetic Pharmacy, Shanxi Institute of Biology, Taiyuan 030006, China.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Cao
authors.affiliation: Room for Genetic Pharmacy, Shanxi Institute of Biology, Taiyuan 030006, China.
authors.forename: Fengmei
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Room for Genetic Pharmacy, Shanxi Institute of Biology, Taiyuan 030006, China.
authors.forename: Quanai
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Room for Genetic Pharmacy, Shanxi Institute of Biology, Taiyuan 030006, China. *Corresponding authors, E-mail: zhaoyisws@163.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects*; D015153:Blotting, Western; D002460:Cell Line; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D013925:Thromboplastin / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D013925:Thromboplastin