title: Anticoagulation and endothelial cell behaviors of heparin-loaded graphene oxide coating on titanium surface.
abstract: Owing to its unique physical and chemical properties, graphene oxide (GO) has attracted tremendous interest in many fields including biomaterials and biomedicine. The purpose of the present study is to investigate the endothelial cell behaviors and anticoagulation of heparin-loaded GO coating on the titanium surface. To this end, the titanium surface was firstly covered by the polydopamine coating followed by the deposition of the GO coating. Heparin was finally loaded on the GO coating to improve the blood compatibility. The results of attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), Raman spectroscopy and X-ray photoelectron spectroscopy (XPS) indicated that the heparin-loaded GO coating was successfully created on the titanium surface. The scanning electron microscopy (SEM) images indicated that a relative uniform GO coating consisting of multilayer GO sheets was formed on the substrate. The hydrophilicity of the titanium surface was enhanced after the deposition of GO and further improved significantly by the loading heparin. The GO coating can enhance the endothelial cell adhesion and proliferation as compared with polydopamine coating and the blank titanium. Loading heparin on the GO coating can significantly reduce the platelet adhesion and prolong the activated partial thromboplastin time (APTT) while not influence the endothelial cell adhesion and proliferation. Therefore, the heparin-loaded GO coating can simultaneously enhance the cytocompatibility to endothelial cells and blood compatibility of biomaterials. Because the polydopamine coating can be easily prepared on most of biomaterials including polymer, ceramics and metal, thus the approach of the present study may open up a new window of promising an effective and efficient way to promote endothelialization and improve the blood compatibility of blood-contact biomedical devices such as intravascular stents.
authors.affiliation: Jiangsu Provincial Key Laboratory for Interventional Medical Devices, Huaiyin Institute of Technology, Huai'an 223003, China. Electronic address: panchangjiang@hyit.edu.cn.
authors.forename: Chang-Jiang
authors.initials: CJ
authors.lastname: Pan
authors.affiliation: Department of General Surgery, Huai'an First People's Hospital, Nanjing Medical University, Huai'an 223300, China.
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Pang
authors.affiliation: Zhejiang Zylox Medical Devices Co., Ltd., Hangzhou 310000, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.affiliation: Jiangsu Provincial Key Laboratory for Interventional Medical Devices, Huaiyin Institute of Technology, Huai'an 223003, China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Wang
authors.affiliation: Jiangsu Provincial Key Laboratory for Interventional Medical Devices, Huaiyin Institute of Technology, Huai'an 223003, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.affiliation: Jiangsu Provincial Key Laboratory for Interventional Medical Devices, Huaiyin Institute of Technology, Huai'an 223003, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ye
authors.affiliation: Jiangsu Provincial Key Laboratory for Interventional Medical Devices, Huaiyin Institute of Technology, Huai'an 223003, China.
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Hou
mesh_terms: D001777:Blood Coagulation / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D006108:Graphite / Q000737:chemistry*; D006493:Heparin / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D007211:Indoles / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D010087:Oxides / Q000737:chemistry; D010314:Partial Thromboplastin Time; D056951:Photoelectron Spectroscopy; D010973:Platelet Adhesiveness / Q000187:drug effects; D011108:Polymers / Q000737:chemistry; D013059:Spectrum Analysis, Raman; D013499:Surface Properties; D014025:Titanium
chemical_list: D020099:Coated Materials, Biocompatible; D007211:Indoles; D010087:Oxides; D011108:Polymers; C568283:polydopamine; D006108:Graphite; D006493:Heparin; D014025:Titanium
keywords: Blood compatibility; Endothelial cell; Graphene oxide; Surface coating