title: [Changes in the Expression of Dopaminergic Genes in Brain Structures of Male Mice Exposed to Chronic Social Defeat Stress: An RNA-seq Study].
abstract: Whole-transcriptome analysis (RNA-seq) has been used to analyze changes in the expression of dopaminergic genes that encode proteins involved in the synthesis, inactivation, and neurotransmission of dopamine in the striatum, ventral tegmental area, raphe nuclei of the midbrain, hypothalamus, and hippocampus of male mice subjected to chronic social defeat. The expression of Th, Ddc, and Slc6A3 (Dat1) was upregulated, while that of Ppp1r1b and Sncg was downregulated in the ventral tegmental area; the expression of Th, Ddc, Drd2, and Sncg was downregulated in the raphe nuclei of midbrain; the expression of Th, Aldh2, and Ppp1r1b was upregulated, while that of Маоа was downregulated in the hypothalamus; Drd1 and Snca expression was downregulated and that of Sncb was upregulated in the striatum, and Sncb expression was upregulated in the hippocampus. There were no statistically significant changes in the expression of Comt, Maob, Drd3, Drd4, or Drd5 in the brain areas analyzed in stressed male mice (compared to control animals). Thus, the number of differentially expressed dopaminergic genes and the direction of expression changes in male mice experiencing chronic stress are specific to regions of the brain.
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090 Russia.
authors.forename: I L
authors.initials: IL
authors.lastname: Kovalenko
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090 Russia.
authors.forename: D A
authors.initials: DA
authors.lastname: Smagin
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090 Russia.
authors.forename: A G
authors.initials: AG
authors.lastname: Galyamina
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090 Russia.
authors.forename: Yu L
authors.initials: YL
authors.lastname: Orlov
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090 Russia.|n.n.kudryavtseva@gmail.com.
authors.forename: N N
authors.initials: NN
authors.lastname: Kudryavtseva
mesh_terms: D000071396:Aldehyde Dehydrogenase, Mitochondrial / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002394:Catechol O-Methyltransferase; D004298:Dopamine; D050483:Dopamine Plasma Membrane Transport Proteins / Q000378:metabolism; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32 / Q000378:metabolism; D008297:Male; D051379:Mice; D017448:Receptors, Dopamine D2; D050637:Receptors, Dopamine D3; D050638:Receptors, Dopamine D4; D050641:Receptors, Dopamine D5; D017423:Sequence Analysis, RNA; D013315:Stress, Psychological; D051845:gamma-Synuclein / Q000378:metabolism
chemical_list: C000600618:DRD2 protein, mouse; D050483:Dopamine Plasma Membrane Transport Proteins; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; C493594:Drd3 protein, mouse; C493600:Drd4 protein, mouse; C493606:Drd5 protein, mouse; C497929:Ppp1r1b protein, mouse; D017448:Receptors, Dopamine D2; D050637:Receptors, Dopamine D3; C493215:Slc6a3 protein, mouse; D051845:gamma-Synuclein; D050638:Receptors, Dopamine D4; D050641:Receptors, Dopamine D5; C515029:ALDH2 protein, mouse; D000071396:Aldehyde Dehydrogenase, Mitochondrial; C000593198:COMT protein, mouse; D002394:Catechol O-Methyltransferase; D004298:Dopamine
keywords: dopaminergic genes; social stress; transcriptome