title: Overexpression and Biological Function of Ubiquitin-Specific Protease 42 in Gastric Cancer.
abstract: Ubiquitin-specific protease 42 (USP42) is a member of deubiquitinating enzymes (DUBs). The alterations of DUBs are implicated in the pathogenesis of a wide variety of tumors. However, there are few studies on the expression and biological function of USP42 in gastric cancer (GC). Here, the expression levels of USP42 were significantly higher in GC tissues than in non-tumorous tissues. USP42 expression was significantly correlated with tumor size, TNM stage, lymph node metastasis and overall survival of patients with GC. Moreover, USP42 silencing in two GC cell lines, AGS and MKN-45, notably inhibited cell proliferation, but stimulated G1 phase arrest. The proteins promoting cell cycle progression (Cyclin D1, Cyclin E1 and PCNA) were down-regulated in USP42-suppressed cells. Moreover, inhibition of USP42 in GC cells impaired cell invasion via affecting the expression of matrix metalloproteinases (MMPs) and epithelial-mesenchymal transition (EMT) regulators. In conclusion, USP42 overexpression could be a potential prognostic marker for GC, regulate the survival and invasive properties of GC, and may represent a novel therapeutic molecular target for this tumor.
authors.affiliation: Department of General Surgery, Shanghai Pudong District People's Hospital, Shanghai 201299, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Hou
authors.affiliation: Department of General Surgery, Shanghai Pudong District People's Hospital, Shanghai 201299, China.
authors.forename: Zhenya
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Department of General Surgery, Punan Hospital, Pudong New District, Shanghai 200125, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Shanghai Pudong District People's Hospital, Shanghai 201299, China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Shanghai Pudong District People's Hospital, Shanghai 201299, China.
authors.forename: Shiyong
authors.initials: S
authors.lastname: Yu
authors.affiliation: Department of General Surgery, Shanghai Pudong District People's Hospital, Shanghai 201299, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: Department of General Surgery, Shanghai Pudong District People's Hospital, Shanghai 201299, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yan
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D045744:Cell Line, Tumor; D018572:Disease-Free Survival; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000401:mortality* / Q000473:pathology; D015996:Survival Rate; D013869:Thiolester Hydrolases / Q000096:biosynthesis*
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; C508707:USP42 protein, human; D013869:Thiolester Hydrolases