title: Combination of Bioactive Polymeric Membranes and Stem Cells for Periodontal Regeneration: In Vitro and In Vivo Analyses.
abstract: Regeneration of periodontal tissues requires a concerted effort to obtain consistent and predictable results in vivo. The aim of the present study was to test a new family of bioactive polymeric membranes in combination with stem cell therapy for periodontal regeneration. In particular, the novel polyester poly(isosorbide succinate-co-L-lactide) (PisPLLA) was compared with poly(L-lactide) (PLLA). Both polymers were combined with collagen (COL), hydroxyapatite (HA) and the growth factor bone morphogenetic protein-7 (BMP7), and their osteoinductive capacity was evaluated via in vitro and in vivo experiments. Membranes composed of PLLA/COL/HA or PisPLLA/COL/HA were able to promote periodontal regeneration and new bone formation in fenestration defects in rat jaws. According to quantitative real-time polymerase chain reaction (qRT-PCR) and Alizarin Red assays, better osteoconductive capacity and increased extracellular mineralization were observed for PLLA/COL/HA, whereas better osteoinductive properties were associated with PisPLLA/COL/HA. We concluded that membranes composed of either PisPLLA/COL/HA or PLLA/COL/HA present promising results in vitro as well as in vivo and that these materials could be potentially applied in periodontal regeneration.
authors.affiliation: Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brasil, 05508-000.
authors.forename: Flávia
authors.initials: F
authors.lastname: Gonçalves
authors.affiliation: Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brasil, 05508-090.
authors.forename: Míriam Santos
authors.initials: MS
authors.lastname: de Moraes
authors.affiliation: Departamento de Biomateriais e Biologia Oral, Faculdade de Odontologia, Universidade de São Paulo, São Paulo, SP, Brasil, 05508-000.
authors.forename: Lorraine Braga
authors.initials: LB
authors.lastname: Ferreira
authors.affiliation: NUCEL/NETCEM-Núcleo de Terapia Celular e Molecular, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil, 05360-130.
authors.forename: Ana Cláudia Oliveira
authors.initials: AC
authors.lastname: Carreira
authors.affiliation: NUCEL/NETCEM-Núcleo de Terapia Celular e Molecular, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil, 05360-130.
authors.forename: Patrícia Mayumi
authors.initials: PM
authors.lastname: Kossugue
authors.affiliation: Departamento de Biomateriais e Biologia Oral, Faculdade de Odontologia, Universidade de São Paulo, São Paulo, SP, Brasil, 05508-000.
authors.forename: Letícia Cristina Cidreira
authors.initials: LC
authors.lastname: Boaro
authors.affiliation: Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brasil, 05508-000.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Bentini
authors.affiliation: Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brasil, 05508-090.
authors.forename: Célia Regina da Silva
authors.initials: CR
authors.lastname: Garcia
authors.affiliation: NUCEL/NETCEM-Núcleo de Terapia Celular e Molecular, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil, 05360-130.|Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brasil, 05508-000.
authors.forename: Mari Cleide
authors.initials: MC
authors.lastname: Sogayar
authors.affiliation: Departamento de Biomateriais e Biologia Oral, Faculdade de Odontologia, Universidade de São Paulo, São Paulo, SP, Brasil, 05508-000.
authors.forename: Victor Elias
authors.initials: VE
authors.lastname: Arana-Chavez
authors.affiliation: Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brasil, 05508-000.
authors.forename: Luiz Henrique
authors.initials: LH
authors.lastname: Catalani
mesh_terms: D000818:Animals; D055419:Bone Morphogenetic Protein 7 / Q000737:chemistry; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D003782:Dental Pulp / Q000166:cytology; D017886:Durapatite / Q000737:chemistry; D006801:Humans; D010012:Osteogenesis / Q000187:drug effects; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism; D010519:Periodontium / Q000473:pathology / Q000502:physiology*; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D055419:Bone Morphogenetic Protein 7; D018786:Bone Substitutes; D050659:Core Binding Factor Alpha 1 Subunit; D011091:Polyesters; D011108:Polymers; D053495:Osteopontin; C033616:poly(lactide); D003094:Collagen; D017886:Durapatite