title: Microsatellite DNA typing for assessment of genetic variability in Marwari breed of Indian goat.
abstract: OBJECTIVE
  To estimate existing within-breed genetic variability in Marwari goats under field conditions and the generated data that can be used to determine genetic relationships with other breed of goats.
  
  
  METHODS
  A total of 146 blood samples of goats of Marwari breed were randomly collected from genetically unrelated animals from different villages of Bikaner Districts of Rajasthan, India. Genomic DNA was extracted from whole blood using proteinase K-digestion followed by standard phenol-chloroform extraction procedure at room temperature and confirmed through horizontal electrophoresis on 0.8% agarose gel containing ethidium bromide. Fifteen caprine microsatellite markers were used to estimate genetic variability among the goats of Marwari breed in terms of allelic and genotype frequencies, heterozygosities and polymorphism information content (PIC) value.
  
  
  RESULTS
  A total of 74 alleles were contributed by Marwari goat across all 15 microsatellite loci. The number of alleles per locus varied from two (ILSTS-087) to 9 (ILSTS-058) alleles, with a mean of 4.93 whereas the effective number of allele varied from 1.35 (ILSTS-005) to 3.129 (ILSTS011) with a mean of 2.36. The effective number of allele is lesser than observed number at all the loci. Allelic sizes ranged from 125 bp (ILSTS-028 and ILSTS-033) to 650 bp (ILSTS-011 and ILSTS-019). The expected heterozygosity ranged from 0.240 (locus ILSTS-005) to 0.681 (locus ILSTS-011), with an average value of 0.544. The observed heterozygosity (Ho) ranged from 0.1428 (locus ILSTS-087) to 0.9285 (locus ILSTS-034), with an average value of 0.5485 indicates substantial and very good number of heterozygotes, in the population. The highest PIC value (1.1886) was observed at ILSTS-044 locus and least (0.0768) at ILSTS-065 locus for Marwari goat.
  
  
  CONCLUSIONS
  Microsatellite analysis revealed a high level of polymorphism across studied microsatellite markers and informativeness of the markers for genetic diversity analysis studies in Marwari goats. This high level of polymorphism can be utilized to plan future biodiversity studies to exploit the uniqueness and adaptability of this breed to Western Rajasthan. Most studied microsatellite markers proving to be good candidates for genetic characterization and diversity analysis of this breed of goat.
authors.affiliation: Department of Animal Breeding & Genetics, College of Veterinary & Animal Science, Rajasthan University of Veterinary & Animal Science, Bikaner - 334 003, Rajasthan, India.
authors.forename: Anoop Singh
authors.initials: AS
authors.lastname: Yadav
authors.affiliation: Department of Animal Breeding & Genetics, College of Veterinary & Animal Science, Rajasthan University of Veterinary & Animal Science, Bikaner - 334 003, Rajasthan, India.
authors.forename: Kritika
authors.initials: K
authors.lastname: Gahlot
authors.affiliation: Department of Animal Breeding & Genetics, College of Veterinary & Animal Science, Rajasthan University of Veterinary & Animal Science, Bikaner - 334 003, Rajasthan, India.
authors.forename: Gyan Chand
authors.initials: GC
authors.lastname: Gahlot
authors.affiliation: Department of Animal Breeding & Genetics, College of Veterinary & Animal Science, Rajasthan University of Veterinary & Animal Science, Bikaner - 334 003, Rajasthan, India.
authors.forename: Mohd
authors.initials: M
authors.lastname: Asraf
authors.affiliation: Department of Animal Breeding & Genetics, College of Veterinary & Animal Science, Rajasthan University of Veterinary & Animal Science, Bikaner - 334 003, Rajasthan, India.
authors.forename: Mohan Lal
authors.initials: ML
authors.lastname: Yadav
keywords: Marwari goats; allelic frequency; heterozygosity; microsatellite marker; polymorphism information content