title: Adipose triglyceride lipase decrement affects skeletal muscle homeostasis during aging through FAs-PPARα-PGC-1α antioxidant response.
abstract: During aging skeletal muscle shows an accumulation of oxidative damage as well as intramyocellular lipid droplets (IMLDs). However, although the impact of these modifications on muscle tissue physiology is well established, the direct effectors critical for their occurrence are poorly understood. Here we show that during aging the main lipase of triacylglycerols, ATGL, significantly declines in gastrocnemius and its downregulation in C2C12 myoblast leads to the accumulation of lipid droplets. Indeed, we observed an increase of oxidative damage to proteins in terms of carbonylation, S-nitrosylation and ubiquitination that is dependent on a defective antioxidant cell response mediated by ATGL-PPARα-PGC-1α. Overall our findings describe a pivotal role for ATGL in the antioxidant/anti-inflammatory response of muscle cells highlighting this lipase as a therapeutic target for fighting the progressive decline in skeletal muscle mass and strength.
authors.affiliation: Department of Biology, University of Rome 'Tor Vergata', Rome, Italy.|IRCCS San Raffaele 'La Pisana', Rome, Italy.
authors.forename: Katia
authors.initials: K
authors.lastname: Aquilano
authors.affiliation: Università Telematica San Raffaele Roma, Rome, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Baldelli
authors.affiliation: Department of Biology, University of Rome 'Tor Vergata', Rome, Italy.
authors.forename: Livia
authors.initials: L
authors.lastname: La Barbera
authors.affiliation: Department of Biology, University of Rome 'Tor Vergata', Rome, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Lettieri Barbato
authors.affiliation: IRCCS San Raffaele 'La Pisana', Rome, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Tatulli
authors.affiliation: Department of Biology, University of Rome 'Tor Vergata', Rome, Italy.|IRCCS San Raffaele 'La Pisana', Rome, Italy.
authors.forename: Maria Rosa
authors.initials: MR
authors.lastname: Ciriolo
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D006706:Homeostasis / Q000502:physiology*; D008049:Lipase / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology*; D010084:Oxidation-Reduction; D047493:PPAR alpha / Q000378:metabolism*; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / Q000378:metabolism*; D050050:Protein Carbonylation; D054875:Ubiquitination; D000217:Acyltransferases
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D005227:Fatty Acids; D008049:Lipase; D047493:PPAR alpha; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C485290:Ppargc1a protein, mouse; C494445:PNPLA2 protein, mouse; D000217:Acyltransferases
keywords: Gerotarget; antioxidants; inflammation; lipid metabolism; myoblasts; oxidative stress