title: Apyrase Elicits Host Antimicrobial Responses and Resolves Infection in Burns.
abstract: The authors previously reported that adenosine triphosphate (ATP) stimulates biofilm formation and removal of the ATP could reduce biofilm formation. The main objective of this study was to evaluate the effects of the ATP-hydrolyzing enzyme, apyrase, on control of Acinetabacter baumannii infection in the burn wound as well as to assess host skin antimicrobial responses. The authors found that apyrase stimulated nitric oxide formation at the wound site and reduced CD55 expression, thereby inducing the assembly of membrane attack complexes. Apyrase treatment nearly eradicated multidrug-resistant A. baumannii from burn wounds in the absence of antibiotics. Apyrase may be an effective therapy against antibiotic-resistant bacterial infections in burns.
authors.affiliation: From the *Departments of Internal Medicine, †Environmental Health Sciences, ‡Surgery, University of Michigan, Ann Arbor; and §Department of Medicine, VA Ann Arbor Health Systems, Michigan.
authors.forename: Jill M
authors.initials: JM
authors.lastname: Bayliss
authors.forename: Benjamin
authors.initials: B
authors.lastname: Levi
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Wu
authors.forename: Stewart C
authors.initials: SC
authors.lastname: Wang
authors.forename: Grace L
authors.initials: GL
authors.lastname: Su
authors.forename: Chuanwu
authors.initials: C
authors.lastname: Xi
mesh_terms: D000151:Acinetobacter Infections / Q000188:drug therapy* / Q000276:immunology; D040981:Acinetobacter baumannii; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents; D001081:Apyrase / Q000627:therapeutic use*; D002056:Burns / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial; D005260:Female; D008810:Mice, Inbred C57BL; D014946:Wound Infection / Q000188:drug therapy* / Q000276:immunology
chemical_list: D000900:Anti-Bacterial Agents; D000255:Adenosine Triphosphate; D001081:Apyrase