title: Differentiation and Characterization of Tr1 Cells.
abstract: Regulatory T cell-mediated suppression serves as a pivotal mechanism of negative regulation of immune-mediated inflammation. Type 1 regulatory T cells (Tr1 cells) are an important subset of CD4+ T cells that prevent excessive inflammatory responses and maintain immune tolerance. The anti-inflammatory role of Tr1 cells is mediated in part by their production of interleukin 10 (IL-10), which dampens the function of both antigen-presenting cells and antigen-specific effector T cells. Additionally, Tr1 cells can kill effector and myeloid cells through the perforin-granzyme B pathway. Adoptive transfer of in vitro differentiated Tr1 cells can be used to suppress autoimmune tissue inflammation in vivo. This unit describes the in vitro stimulation of naïve murine CD4+ T cells using IL-27 to generate IL-10-producing Tr1 cells.
authors.affiliation: Evergrande Center for Immunologic Diseases and Ann Romney Center for Neurologic Diseases, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts.
authors.forename: Norio
authors.initials: N
authors.lastname: Chihara
authors.affiliation: Evergrande Center for Immunologic Diseases and Ann Romney Center for Neurologic Diseases, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts.
authors.forename: Asaf
authors.initials: A
authors.lastname: Madi
authors.affiliation: Evergrande Center for Immunologic Diseases and Ann Romney Center for Neurologic Diseases, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Karwacz
authors.affiliation: Translational Health Science and Technology Institute (an autonomous institute of Department of Biotechnology, Govt. of India), Faridabad, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Awasthi
authors.affiliation: Evergrande Center for Immunologic Diseases and Ann Romney Center for Neurologic Diseases, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts.
authors.forename: Vijay K
authors.initials: VK
authors.lastname: Kuchroo
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D015870:Gene Expression; D016130:Immunophenotyping; D066298:In Vitro Techniques; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D016753:Interleukin-10
keywords: CD4; IL-10; IL-27; T cell differentiation; Tr1