title: Rutin ameliorates kidney interstitial fibrosis in rats with obstructive nephropathy.
abstract: Rutin reportedly conveys many beneficial effects, including renoprotection; however, it has not yet been demonstrated to have a renoprotective effect against obstructive nephropathy. The present study is the first to show a protective effect of rutin against obstructive renal injury induced by unilateral ureteral obstruction (UUO). A total of 24 male Wistar rats were randomly divided into four groups of six rats each, including vehicle- or rutin-treated sham operated groups, and vehicle- or rutin-treated UUO groups. Rats received daily oral gavage of rutin (100mg/kg) for 2weeks. All rats were euthanized on postoperative day 14. Histological findings showed that rutin administration significantly reduced renal interstitial injury and suppressed interstitial collagen deposits in UUO rats. Moreover, rutin decreased macrophage infiltration, proinflammatory cytokine expression and phosphorylation of nuclear factor-κB p65. Furthermore, rutin inhibited extracellular matrix accumulation by reducing expression of type I/III collagen and fibronectin. Rutin also prevented the epithelial-mesenchymal transition processes of renal tubular cells by decreasing α-smooth muscle actin expression and retaining E-cadherin expression. These effects of rutin were in parallel with the reductions in Smad3 activity and pivotal to the fibrogenic potential of TGF-β1. Taken together, the renoprotective effects of rutin in obstructive nephropathy were likely due to anti-inflammatory effects and inhibition of TGF-β1/Smad3 signaling.
authors.affiliation: Department of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
authors.forename: Qiu-Hua
authors.initials: QH
authors.lastname: Zhu
authors.affiliation: Department of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
authors.forename: Li-Pei
authors.initials: LP
authors.lastname: Fan
authors.affiliation: Department of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
authors.forename: Liang-Sheng
authors.initials: LS
authors.lastname: Yue
authors.affiliation: Department of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
authors.forename: Liu-Yang
authors.initials: LY
authors.lastname: Li
authors.affiliation: Department of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China. Electronic address: zhaoming0928@126.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects; D005109:Extracellular Matrix / Q000187:drug effects; D005355:Fibrosis; D018836:Inflammation Mediators / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D007674:Kidney Diseases / Q000188:drug therapy*; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012431:Rutin / Q000627:therapeutic use*; D015398:Signal Transduction / Q000187:drug effects; D051900:Smad3 Protein / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014517:Ureteral Obstruction / Q000188:drug therapy*
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D018836:Inflammation Mediators; D051900:Smad3 Protein; C497791:Smad3 protein, mouse; D053773:Transforming Growth Factor beta1; D012431:Rutin
keywords: Inflammation; Rutin; Tubulointerstitial fibrosis; UUO