title: Synthesis of a hybrid type N-glycan heptasaccharide oxazoline for Endo M catalysed glycosylation.
abstract: Endo-β-N-acetylglucosaminidases (ENGases) are versatile biocatalysts that allow access to a wide variety of defined homogenous N-linked glycoconjugates in a convergent manner. A hybrid-type N-glycan was accessed by total synthesis, converted to an oxazoline, and used as a donor substrate with both wild type Endo M and an N175Q glycosynthase Endo M mutant allowing the convergent synthesis of a glycosylated amino acid bearing a hybrid N-glycan structure.
authors.affiliation: Department of Chemistry, University of Canterbury, Private Bag 4800, Christchurch 8140, New Zealand.
authors.forename: Pragya
authors.initials: P
authors.lastname: Priyanka
authors.affiliation: Department of Chemistry, University of Canterbury, Private Bag 4800, Christchurch 8140, New Zealand; Biomolecular Interaction Centre, University of Canterbury, Private Bag 4800, Christchurch 8140, New Zealand. Electronic address: antony.fairbanks@canterbury.ac.nz.
authors.forename: Antony J
authors.initials: AJ
authors.lastname: Fairbanks
mesh_terms: D055162:Biocatalysis; D002236:Carbohydrate Conformation; D006031:Glycosylation; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010080:Oxazoles / Q000737:chemistry* / Q000378:metabolism*; D011134:Polysaccharides / Q000096:biosynthesis* / Q000737:chemistry*
chemical_list: D010080:Oxazoles; D011134:Polysaccharides; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
keywords: ENGase; Endo M; Endo-β-N-acetylglucosaminidase; Endohexosaminidase; Hybrid N-glycan; Oxazoline