title: Identification and Characterization of CD133(pos) Subpopulation Cells From a Human Laryngeal Cancer Cell Line.
abstract: BACKGROUND
  Recent research indicates that CD133 are expressed in several kinds of stem cells, among which, its high expression in laryngeal carcinoma has caused wide concern. To further explore efficaciously targeting drugs to laryngeal carcinoma stem cells (CSCs), we transplanted a solid tumor from CSCs into abdominal subcutaneous tissue of nude mice, and then compared the biological characteristics of laryngeal solid tumors with or without cisplatin intervention.
  
  
  METHODS
  In this study, the expression of CD133 was detected in the Hep-2 cell line by flow cytometry. By applying magnetic cell sorting (MACS) technology, we reported the results of purifying CD133-positive cells from a Hep-2 cell line. Cell proliferation, colony formation, and tumor-forming ability were examined in vitro and in vivo to identify the marker of CSCs in Hep-2 cell line.
  
  
  RESULTS
  Upon flow cytometry analysis, CD133 was expressed constantly on 40.12±1.32% in Hep-2 cell line. Cell proliferation and colony formation ability were higher in CD133-positive cells compared to CD133-negative cells, and the in vivo tumorigenesis experiment showed the same results as in vitro assay. The 2 subpopulations cells were both sensitive to DDP, among which, the effect of DPP on proliferation ability and tumor-forming ability of CD133-positive cells was obviously greater than that of CD133-negative cells.
  
  
  CONCLUSIONS
  Above all, our study revealed that CD133-positive cells have properties of higher proliferation, colony formation, and tumorigenesis in Hep-2 cell line, indicating that CD133 could be a marker to characterize laryngeal cancer stem cells.
authors.affiliation: Departments of Otolaryngology, Tongji Hospital, Tongji University School of Medicine, Shanghai, China (mainland).
authors.forename: Hai-ou
authors.initials: HO
authors.lastname: Qiu
authors.affiliation: Departments of Nephrology, Tongji Hospital, Tongji University School of Medicine, Shanghai, China (mainland).
authors.forename: Huifang
authors.initials: H
authors.lastname: Wang
authors.affiliation: Departments of Otolaryngology, Tongji Hospital, Tongji University School of Medicine, Shanghai, China (mainland).
authors.forename: Na
authors.initials: N
authors.lastname: Che
authors.affiliation: Departments of Clinical Laboratory, Tongji Hospital, Tongji University School of Medicine, Shanghai, China (mainland).
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.affiliation: Departments of Otolaryngology, Tongji Hospital, Tongji University School of Medicine, Shanghai, China (mainland).
authors.forename: Yong
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Departments of Translational Center for Stem Cell Research, Tongji Hospital, Tongji University School of Medicine, Shanghai, China (mainland).
authors.forename: Qiao
authors.initials: Q
authors.lastname: Zeng
authors.affiliation: Departments of Otolaryngology, Tongji Hospital, Tongji University School of Medicine, Shanghai, China (mainland).
authors.forename: Rongming
authors.initials: R
authors.lastname: Ge
mesh_terms: D000071916:AC133 Antigen / Q000378:metabolism*; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D002945:Cisplatin / Q000494:pharmacology / Q000627:therapeutic use; D005434:Flow Cytometry; D006801:Humans; D007822:Laryngeal Neoplasms / Q000188:drug therapy / Q000473:pathology*; D060328:Magnetic Phenomena; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D047368:Tumor Burden / Q000187:drug effects; D014410:Tumor Stem Cell Assay
chemical_list: D000071916:AC133 Antigen; D002945:Cisplatin