title: The effects of melatonin on endometriotic lesions induced by implanting human endometriotic cells in the first SCID-mouse endometriosis-model developed in Turkey.
abstract: OBJECTIVE
  To evaluate the effects of melatonin on endometriotic lesions induced by implanting human endometriotic cells in SCID mice.
  
  
  METHODS
  Prospective, randomized, controlled, experimental study. Experimental Research Center of Yeditepe University (YUDETAM). Thirty female, non-pregnant, nulligravid severe combined immunodeficient (SCID) mice. Endometriotic cells collected from patients with endometriosis were implanted subcutaneously in 30 SCID mice. These mice were randomized into two study groups: in the first group, mice were administered melatonin (20 mg/kg/day) following induction of endometriosis for four weeks; in the second group, nothing was administered. All the mice were given a high dose of exogenous estradiol (50 µg/kg/d, twice weekly). Four weeks after inoculation, necropsies were performed and endometriotic lesions were collected. All the lesions were evaluated histopathologically and the levels of SOD and MDA were assessed in the lesions.
  
  
  RESULTS
  Successful implantation was observed in the 28 mice that survived. Mean MDA level was 5.0 ± 1.7 and 8.8 ± 2.6 in the melatonin and control groups, respectively (p = 0.01); mean SOD level was 1.1 ± 0.1 and 1.0 ± 0.1 in the melatonin and control groups, respectively (p = 0.49). Mean histopathological score was lower in the melatonin group (p = 0.04).
  
  
  CONCLUSIONS
  Melatonin was effective in the treatment of experimental endometriosis induced in SCID mice.
authors.forename: N
authors.initials: N
authors.lastname: Yesildaglar
authors.forename: G
authors.initials: G
authors.lastname: Yildirim
authors.forename: O K
authors.initials: OK
authors.lastname: Yildirim
authors.forename: R
authors.initials: R
authors.lastname: Attar
authors.forename: F
authors.initials: F
authors.lastname: Ozkan
authors.forename: H
authors.initials: H
authors.lastname: Akkaya
authors.forename: B
authors.initials: B
authors.lastname: Yilmaz
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D004195:Disease Models, Animal; D004715:Endometriosis / Q000209:etiology / Q000473:pathology / Q000628:therapy*; D004958:Estradiol / Q000627:therapeutic use; D005260:Female; D008550:Melatonin / Q000494:pharmacology*; D051379:Mice; D016513:Mice, SCID; D011247:Pregnancy; D011270:Pregnancy, Animal; D011446:Prospective Studies; D014421:Turkey
chemical_list: D000975:Antioxidants; D004958:Estradiol; D008550:Melatonin