title: Solid and liquid media for isolating and cultivating acidophilic and acid-tolerant sulfate-reducing bacteria.
abstract: Growth media have been developed to facilitate the enrichment and isolation of acidophilic and acid-tolerant sulfate-reducing bacteria (aSRB) from environmental and industrial samples, and to allow their cultivation in vitro The main features of the 'standard' solid and liquid devised media are as follows: (i) use of glycerol rather than an aliphatic acid as electron donor; (ii) inclusion of stoichiometric concentrations of zinc ions to both buffer pH and to convert potentially harmful hydrogen sulphide produced by the aSRB to insoluble zinc sulphide; (iii) inclusion of Acidocella aromatica (an heterotrophic acidophile that does not metabolize glycerol or yeast extract) in the gel underlayer of double layered (overlay) solid media, to remove acetic acid produced by aSRB that incompletely oxidize glycerol and also aliphatic acids (mostly pyruvic) released by acid hydrolysis of the gelling agent used (agarose). Colonies of aSRB are readily distinguished from those of other anaerobes due to their deposition and accumulation of metal sulphide precipitates. Data presented illustrate the effectiveness of the overlay solid media described for isolating aSRB from acidic anaerobic sediments and low pH sulfidogenic bioreactors.
authors.affiliation: School of Biological Sciences, College of Natural Sciences, Bangor University, Bangor LL57 2UW, UK.
authors.forename: Ivan
authors.initials: I
authors.lastname: Ňancucheo
authors.affiliation: School of Biological Sciences, College of Natural Sciences, Bangor University, Bangor LL57 2UW, UK.
authors.forename: Owen F
authors.initials: OF
authors.lastname: Rowe
authors.affiliation: School of Biological Sciences, College of Natural Sciences, Bangor University, Bangor LL57 2UW, UK.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Hedrich
authors.affiliation: School of Biological Sciences, College of Natural Sciences, Bangor University, Bangor LL57 2UW, UK d.b.johnson@bangor.ac.uk.
authors.forename: D Barrie
authors.initials: DB
authors.lastname: Johnson
mesh_terms: D041801:Acidiphilium / Q000378:metabolism; D020561:Alphaproteobacteria / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry*; D003901:Desulfovibrio / Q000254:growth & development* / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism / Q000494:pharmacology; D006862:Hydrogen Sulfide / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D019289:Pyruvic Acid / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism* / Q000494:pharmacology; D013440:Sulfides / Q000378:metabolism / Q000494:pharmacology; D015032:Zinc / Q000378:metabolism / Q000494:pharmacology; D017967:Zinc Compounds / Q000378:metabolism / Q000494:pharmacology
chemical_list: D003470:Culture Media; D013431:Sulfates; D013440:Sulfides; D017967:Zinc Compounds; D019289:Pyruvic Acid; D015032:Zinc; C031238:zinc sulfide; D005990:Glycerol; D006862:Hydrogen Sulfide
keywords: acidophile; glycerol; hydrogen sulphide; media; sulfate-reducing bacteria; zinc