title: Ultrastructural cytochemical study of enzymes expressed by signet ring cells in gastric cancer.
abstract: OBJECTIVE
  To study the ultrastructural localization of five marker enzymes (ALPase, ACPase, G6Pase, TPPase and CCOase) in gastric cancer signet ring cells to demonstrate their biologic behaviors.
  
  
  METHODS
  Five marker enzymes were examined in signet ring cells of seven gastric cancer patients by ultrastructural enzyme cytochemical techniques.
  
  
  RESULTS
  The number of corresponding organelles and the activities of marker enzymes, especially ACPase and TPPase, increased, leading to stronger mucus synthesis, secretion and digestion in gastric cancer signet ring cells. There was a lack of collagenous fibers in the stroma around the cancer nests.
  
  
  CONCLUSIONS
  Signet ring cell carcinoma is very invasive with metastasis rates due to the secretion of proteolytic enzymes.
authors.affiliation: Guang-Lin Yang, Yu-Ming Dong, Wei-Dong Du, Ying-Hao Su, Hong Zhang, Ji-Fen Wu, Dao-Bin Wang, Ai-Lian Xu, Department of Pathology, Anhui Medical University, Hefei 230032, Anhui Province, China.
authors.forename: G L
authors.initials: GL
authors.lastname: Yang
authors.affiliation: Guang-Lin Yang, Yu-Ming Dong, Wei-Dong Du, Ying-Hao Su, Hong Zhang, Ji-Fen Wu, Dao-Bin Wang, Ai-Lian Xu, Department of Pathology, Anhui Medical University, Hefei 230032, Anhui Province, China.
authors.forename: Y M
authors.initials: YM
authors.lastname: Dong
authors.affiliation: Guang-Lin Yang, Yu-Ming Dong, Wei-Dong Du, Ying-Hao Su, Hong Zhang, Ji-Fen Wu, Dao-Bin Wang, Ai-Lian Xu, Department of Pathology, Anhui Medical University, Hefei 230032, Anhui Province, China.
authors.forename: W D
authors.initials: WD
authors.lastname: Du
authors.affiliation: Guang-Lin Yang, Yu-Ming Dong, Wei-Dong Du, Ying-Hao Su, Hong Zhang, Ji-Fen Wu, Dao-Bin Wang, Ai-Lian Xu, Department of Pathology, Anhui Medical University, Hefei 230032, Anhui Province, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Su
authors.affiliation: Guang-Lin Yang, Yu-Ming Dong, Wei-Dong Du, Ying-Hao Su, Hong Zhang, Ji-Fen Wu, Dao-Bin Wang, Ai-Lian Xu, Department of Pathology, Anhui Medical University, Hefei 230032, Anhui Province, China.
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Guang-Lin Yang, Yu-Ming Dong, Wei-Dong Du, Ying-Hao Su, Hong Zhang, Ji-Fen Wu, Dao-Bin Wang, Ai-Lian Xu, Department of Pathology, Anhui Medical University, Hefei 230032, Anhui Province, China.
authors.forename: J F
authors.initials: JF
authors.lastname: Wu
authors.affiliation: Guang-Lin Yang, Yu-Ming Dong, Wei-Dong Du, Ying-Hao Su, Hong Zhang, Ji-Fen Wu, Dao-Bin Wang, Ai-Lian Xu, Department of Pathology, Anhui Medical University, Hefei 230032, Anhui Province, China.
authors.forename: D B
authors.initials: DB
authors.lastname: Wang
authors.affiliation: Guang-Lin Yang, Yu-Ming Dong, Wei-Dong Du, Ying-Hao Su, Hong Zhang, Ji-Fen Wu, Dao-Bin Wang, Ai-Lian Xu, Department of Pathology, Anhui Medical University, Hefei 230032, Anhui Province, China.
authors.forename: A L
authors.initials: AL
authors.lastname: Xu
keywords: Acid phosphatase/analysis; Alkaline phosphatase/analysis; Carcinoma, signet ring cell/enzymology; Stomach neoplasms/enzymology