title: Knockdown of TC-1 enhances radiosensitivity of non-small cell lung cancer via the Wnt/β-catenin pathway.
abstract: Thyroid cancer 1 (TC-1, C8ofr4) is widely expressed in vertebrates and associated with many kinds of tumors. Previous studies indicated that TC-1 functions as a positive regulator in the Wnt/β-catenin signaling pathway in non-small cell lung cancer (NSCLC). However, its exact role and regulation mechanism in radiosensitivity of NSCLC are still unclear. The expression level of TC-1 was measured by qRT-PCR and western blot in NSCLC cell lines. Proliferation and apoptosis of NSCLC cells in response to TC-1 knockdown or/and radiation were determined by MTT assay and flow cytometry, respectively. The activation of the Wnt/β-catenin signaling pathway was further examined by western blotin vitroandin vivo Compared to TC-1 siRNA or radiotherapy alone, TC-1 silencing combined with radiation inhibited cell proliferation and induced apoptosis in NSCLC cell lines by inactivating of the Wnt/β-catenin signaling pathway. Furthermore, inhibition of the Wnt/β-catenin signaling pathway by XAV939, a Wnt/β-catenin signaling inhibitor, contributed to proliferation inhibition and apoptosis induction in NSCLC A549 cells. Combinative treatment of A549 xenografts with TC-1 siRNA and radiation caused significant tumor regression and inactivation of the Wnt/β-catenin signaling pathway relative to TC-1 siRNA or radiotherapy alone. The results fromin vitroandin vivostudies indicated that TC-1 silencing sensitized NSCLC cell lines to radiotherapy through the Wnt/β-catenin signaling pathway.
authors.affiliation: Department of Radiotherapy, Huaihe Hospital of Henan University, Kaifeng 475000, China dapengwu0521@sina.com.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Respiratory, Huaihe Hospital of Henan University, Kaifeng 475000, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Radiotherapy, Huaihe Hospital of Henan University, Kaifeng 475000, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yan
keywords: NSCLC; Radiosensitivity; TC-1; Wnt/β-catenin