title: Modeling and analysis of collective cell migration in an in vivo three-dimensional environment.
abstract: A long-standing question in collective cell migration has been what might be the relative advantage of forming a cluster over migrating individually. Does an increase in the size of a collectively migrating group of cells enable them to sample the chemical gradient over a greater distance because the difference between front and rear of a cluster would be greater than for single cells? We combined theoretical modeling with experiments to study collective migration of the border cells in-between nurse cells in the Drosophila egg chamber. We discovered that cluster size is positively correlated with migration speed, up to a particular point above which speed plummets. This may be due to the effect of viscous drag from surrounding nurse cells together with confinement of all of the cells within a stiff extracellular matrix. The model predicts no relationship between cluster size and velocity for cells moving on a flat surface, in contrast to movement within a 3D environment. Our analyses also suggest that the overall chemoattractant profile in the egg chamber is likely to be exponential, with the highest concentration in the oocyte. These findings provide insights into collective chemotaxis by combining theoretical modeling with experimentation.
authors.affiliation: Molecular, Cellular and Developmental Biology, University of California Santa Barbara, CA 93106; Department of Biological Chemistry, The Johns Hopkins School of Medicine, Baltimore, MD 21205;
authors.forename: Danfeng
authors.identifier: http://orcid.org/0000-0003-1623-5077
authors.initials: D
authors.lastname: Cai
authors.affiliation: Molecular, Cellular and Developmental Biology, University of California Santa Barbara, CA 93106;
authors.forename: Wei
authors.initials: W
authors.lastname: Dai
authors.affiliation: Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, West Bengal 741252, India;
authors.forename: Mohit
authors.initials: M
authors.lastname: Prasad
authors.affiliation: Molecular, Cellular and Developmental Biology, University of California Santa Barbara, CA 93106; Department of Biological Chemistry, The Johns Hopkins School of Medicine, Baltimore, MD 21205;
authors.forename: Junjie
authors.initials: J
authors.lastname: Luo
authors.affiliation: Department of Chemical Physics, The Weizmann Institute of Science, Rehovot 76100, Israel denise.montell@lifesci.ucsb.edu nir.gov@weizmann.ac.il.
authors.forename: Nir S
authors.initials: NS
authors.lastname: Gov
authors.affiliation: Molecular, Cellular and Developmental Biology, University of California Santa Barbara, CA 93106; Department of Biological Chemistry, The Johns Hopkins School of Medicine, Baltimore, MD 21205; denise.montell@lifesci.ucsb.edu nir.gov@weizmann.ac.il.
authors.forename: Denise J
authors.initials: DJ
authors.lastname: Montell
mesh_terms: D000818:Animals; D048429:Cell Size; D002630:Chemotactic Factors / Q000378:metabolism; D002633:Chemotaxis / Q000502:physiology*; D004330:Drosophila / Q000166:cytology*; D005109:Extracellular Matrix / Q000378:metabolism; D008954:Models, Biological; D009865:Oocytes / Q000502:physiology*; D013499:Surface Properties
chemical_list: D002630:Chemotactic Factors
keywords: cell migration; chemotaxis; theoretical modeling; three-dimensional