title: Optimization of Isomaltooligosaccharide Size Distribution by Acceptor Reaction of Weissella confusa Dextransucrase and Characterization of Novel α-(1→2)-Branched Isomaltooligosaccharides.
abstract: Long-chain isomaltooligosaccharides (IMOs) are promising prebiotics. IMOs were produced by a Weissella confusa dextransucrase via maltose acceptor reaction. The inputs of substrates (i.e., sucrose and maltose, 0.15-1 M) and dextransucrase (1-10 U/g sucrose) were used to control IMO yield and profile. According to response surface modeling, 1 M sucrose and 0.5 M maltose were optimal for the synthesis of longer IMOs, whereas the dextransucrase dosage showed no significant effect. In addition to the principal linear IMOs, a homologous series of minor IMOs were also produced from maltose. As identified by MS(n) and NMR spectroscopy, the minor trisaccharide contained an α-(1→2)-linked glucosyl residue on the reducing residue of maltose and thus was α-d-glucopyranosyl-(1→2)-[α-d-glucopyranosyl-(1→4)]-d-glucopyranose (centose). The higher members of the series were probably formed by the attachment of a single unit branch to linear IMOs. This is the first report of such α-(1→2)-branched IMOs produced from maltose by a dextransucrase.
authors.affiliation: Department of Food and Environmental Sciences, University of Helsinki , P.O. Box 27, FI-00014 University of Helsinki, Finland.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Shi
authors.affiliation: Department of Food and Environmental Sciences, University of Helsinki , P.O. Box 27, FI-00014 University of Helsinki, Finland.
authors.forename: Yaxi
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Department of Food and Environmental Sciences, University of Helsinki , P.O. Box 27, FI-00014 University of Helsinki, Finland.
authors.forename: Minna
authors.initials: M
authors.lastname: Juvonen
authors.affiliation: Department of Food and Environmental Sciences, University of Helsinki , P.O. Box 27, FI-00014 University of Helsinki, Finland.
authors.forename: Päivi
authors.initials: P
authors.lastname: Tuomainen
authors.affiliation: VTT Technical Research Centre of Finland Ltd. , P.O. Box 1000, FI-02044 VTT, Finland.
authors.forename: Ilkka
authors.initials: I
authors.lastname: Kajala
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Guwahati , Guwahati 781 039, Assam, India.
authors.forename: Shraddha
authors.initials: S
authors.lastname: Shukla
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Guwahati , Guwahati 781 039, Assam, India.
authors.forename: Arun
authors.initials: A
authors.lastname: Goyal
authors.affiliation: VTT Technical Research Centre of Finland Ltd. , P.O. Box 1000, FI-02044 VTT, Finland.
authors.forename: Hannu
authors.initials: H
authors.lastname: Maaheimo
authors.affiliation: Department of Food and Environmental Sciences, University of Helsinki , P.O. Box 27, FI-00014 University of Helsinki, Finland.
authors.forename: Kati
authors.initials: K
authors.lastname: Katina
authors.affiliation: Department of Food and Environmental Sciences, University of Helsinki , P.O. Box 27, FI-00014 University of Helsinki, Finland.
authors.forename: Maija
authors.initials: M
authors.lastname: Tenkanen
mesh_terms: D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005964:Glucosyltransferases / Q000378:metabolism*; D009844:Oligosaccharides / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D053719:Tandem Mass Spectrometry; D058836:Weissella / Q000201:enzymology*
chemical_list: D009844:Oligosaccharides; C021705:maltooligosaccharides; D005964:Glucosyltransferases; C020025:dextransucrase
keywords: centose; dextransucrase; isomaltooligosaccharides; maltose acceptor reaction; response surface modeling; α-(1→2)-linkage