title: A novel automatable enzyme-coupled colorimetric assay for endo-1,4-β-glucanase (cellulase).
abstract: endo-1,4-β-Glucanase (endo-cellulase, EC 3.2.1.4) is one of the most widely used enzymes in industry. Despite its importance, improved methods for the rapid, selective, quantitative assay of this enzyme have been slow to emerge. In 2014, a novel enzyme-coupled assay that addressed many of the limitations of the existing assay methodology was reported. This involved the use of a bifunctional substrate chemically derived from cellotriose. Reported herein is a much improved version of this assay employing a novel substrate, namely 4,6-O-(3-ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside. Graphical Abstract Principle of the CELLG5 assay.
authors.affiliation: Megazyme International Ireland, Bray Business Park, Southern Cross Road, Bray, County Wicklow, A98 YV29, Ireland. david@megazyme.com.
authors.forename: David
authors.initials: D
authors.lastname: Mangan
authors.affiliation: Megazyme International Ireland, Bray Business Park, Southern Cross Road, Bray, County Wicklow, A98 YV29, Ireland.
authors.forename: Claudio
authors.initials: C
authors.lastname: Cornaggia
authors.affiliation: Megazyme International Ireland, Bray Business Park, Southern Cross Road, Bray, County Wicklow, A98 YV29, Ireland.
authors.forename: Vincent
authors.initials: V
authors.lastname: McKie
authors.affiliation: Megazyme International Ireland, Bray Business Park, Southern Cross Road, Bray, County Wicklow, A98 YV29, Ireland.
authors.forename: Tadas
authors.initials: T
authors.lastname: Kargelis
authors.affiliation: Megazyme International Ireland, Bray Business Park, Southern Cross Road, Bray, County Wicklow, A98 YV29, Ireland.
authors.forename: Barry V
authors.initials: BV
authors.lastname: McCleary
mesh_terms: D001331:Automation / Q000379:methods*; D002480:Cellulase / Q000032:analysis*; D003124:Colorimetry / Q000379:methods*; D057075:Enzyme Assays / Q000379:methods*
chemical_list: D002480:Cellulase
keywords: Assay; Automation; CELLG5; Cellulase; Colorimetric; endo-1,4-β-Glucanase