title: Experimental infection of IS/885/00-like infectious bronchitis virus in specific pathogen free and commercial broiler chicks.
abstract: Pathogenesis of an IS/885/00-like (IS/885) strain of variant infectious bronchitis virus (IBV) was examined in one day old specific pathogen free (SPF) and commercial broiler chicks. Chicks were humanely euthanized at 3, 6, 9, 12, 15, 21 and 28 days post infection (dpi) for necropsy examination, and tissues were collected for histopathology and virus detection by reverse transcription polymerase chain reaction (RT-PCR). Respiratory clinical signs and gross lesions consisting of tracheal caseous exudate and plugs, and swollen kidneys (with or without) urate deposits were observed in SPF and broiler chicks. The onset of disease developed more slowly and were of lesser severity in broiler compared to SPF chicks, reflecting the inhibitory effects of the IBV maternal-antibodies in the broiler chicks or genetic/strain susceptibility, or both. Head swelling was observed in one infected broiler chick at 15 dpi and the virus was recovered by RT-PCR and isolation. In the IS/885-infected SPF chicks, cystic oviducts were found in two female chicks. IS/885 was isolated from the cystic fluid. Using ELISA, low to moderate levels of the antibodies to IBV was detected in the SPF compared to broiler infected chicks.
authors.affiliation: University of Liverpool, Leahurst Campus, Neston, Cheshire, CH64 7TE, UK; University of Omar Al-Mukhtar, Faculty of Veterinary Medicine, Al-Bayda, Libya.
authors.forename: Faez
authors.initials: F
authors.lastname: Awad
authors.affiliation: University of Liverpool, Leahurst Campus, Neston, Cheshire, CH64 7TE, UK.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Chhabra
authors.affiliation: University of Liverpool, Leahurst Campus, Neston, Cheshire, CH64 7TE, UK.
authors.forename: Anne
authors.initials: A
authors.lastname: Forrester
authors.affiliation: University of Liverpool, Leahurst Campus, Neston, Cheshire, CH64 7TE, UK.
authors.forename: Julian
authors.initials: J
authors.lastname: Chantrey
authors.affiliation: University of Liverpool, Leahurst Campus, Neston, Cheshire, CH64 7TE, UK; NIHR Health Protection Research Unit in Emerging and Zoonotic Infections, Liverpool, UK.
authors.forename: Matthew
authors.initials: M
authors.lastname: Baylis
authors.affiliation: Merial S.A.S., 29 avenue Tony Garnier, Lyon cedex 07, France.
authors.forename: Stephane
authors.initials: S
authors.lastname: Lemiere
authors.affiliation: Cairo University, Department of Virology, Faculty of Veterinary Medicine, Cairo, Egypt.
authors.forename: Hussein Aly
authors.initials: HA
authors.lastname: Hussein
authors.affiliation: University of Liverpool, Leahurst Campus, Neston, Cheshire, CH64 7TE, UK. Electronic address: gana@liverpool.ac.uk.
authors.forename: Kannan
authors.initials: K
authors.lastname: Ganapathy
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002645:Chickens; D018352:Coronavirus Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D001351:Infectious bronchitis virus / Q000502:physiology*; D011201:Poultry Diseases / Q000276:immunology* / Q000821:virology; D011897:Random Allocation; D013047:Specific Pathogen-Free Organisms; D014018:Tissue Distribution
chemical_list: D000914:Antibodies, Viral
keywords: Chicks; Cystic oviduct; Head swelling; IBV IS/885/00-like; Infectious bronchitis virus