title: Phosphatidylserine Synthase Controls Cell Elongation Especially in the Uppermost Internode in Rice by Regulation of Exocytosis.
abstract: The uppermost internode is one of the fastest elongating organs in rice, and is expected to require an adequate supply of cell-wall materials and enzymes to the cell surface to enhance mechanical strength. Although it has been reported that the phenotype of shortened uppermost internode 1 (sui1) is caused by mutations in PHOSPHATIDYLSERINE SYNTHASE (OsPSS), the underlying mechanism remains unclear. Here we show that the OsPSS-1, as a gene expressed predominantly in elongating cells, regulates post-Golgi vesicle secretion to intercellular spaces. Mutation of OsPSS-1 leads to compromised delivery of CESA4 and secGFP towards the cell surface, resulting in weakened intercellular adhesion and disorganized cell arrangement in parenchyma. The phenotype of sui1-4 is caused largely by the reduction in cellulose contents in the whole plant and detrimental delivery of pectins in the uppermost internode. We found that OsPSS-1 and its potential product PS (phosphatidylserine) localized to organelles associated with exocytosis. These results together suggest that OsPSS-1 plays a potential role in mediating cell expansion by regulating secretion of cell wall components.
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Ma
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: School of Life Sciences, Centre for Cell and Developmental Biology, Chinese University of Hong Kong, New Territories, Hong Kong, China.
authors.forename: Jinbo
authors.initials: J
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Baocai
authors.initials: B
authors.lastname: Zhang
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Ren
authors.affiliation: School of Life Sciences, Centre for Cell and Developmental Biology, Chinese University of Hong Kong, New Territories, Hong Kong, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Yihua
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, China.
authors.forename: Kunneng
authors.initials: K
authors.lastname: Zhou
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Jiu-Lin
authors.initials: JL
authors.lastname: Wang
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Cailin
authors.initials: C
authors.lastname: Lei
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Xiuping
authors.initials: X
authors.lastname: Guo
authors.affiliation: National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, China.
authors.forename: He
authors.initials: H
authors.lastname: Gao
authors.affiliation: National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, China.
authors.forename: Yiqun
authors.initials: Y
authors.lastname: Bao
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.|National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, China.
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Wan
mesh_terms: D010717:CDPdiacylglycerol-Serine O-Phosphatidyltransferase / Q000235:genetics* / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism; D005089:Exocytosis; D009154:Mutation; D015388:Organelles / Q000378:metabolism; D012275:Oryza / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D010368:Pectins; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D010368:Pectins; D002482:Cellulose; D010717:CDPdiacylglycerol-Serine O-Phosphatidyltransferase