title: Calcium channels contribute to albiflorin-mediated antinociceptive effects in mouse model.
abstract: Albiflorin (AF), one of important bioactive constituents of Paeonia lactiflora Radix, possesses neuro-protective effect. The present study aims to investigate the antinociceptive activities of AF and possible mechanisms. AF suppressed acetic acid-caused writhing, lengthened the latency period of mouse in hot plate test, and reduced the licking and biting response time of the injected mouse paw during phase I and phase II, and it suggested that AF exerted the antinociceptive activity mainly through central nervous system. Nimodipine, a commonly used calcium channels blocker, strongly lengthened AF-enhanced latency period of mouse in hot plate test. Compared with control group, AF reduced the levels of euronal nitric oxide synthase (nNOS), and enhanced the levels of serotonin (5-HT) in serum and/or hypothalamus before and after 30-s thermal stimuli. The reduced activation of calmodulin-dependent protein kinase II and c-Jun N-terminal kinase in hypothalamus was observed in AF-treated mice. Collectively, AF-mediated antinociceptive activities were, at least partially, related to calcium channels.
authors.affiliation: Department of Neurology, The Second Hospital of Jilin University, Jilin University, Changchun 130041, China; Department of Biomedicine, Institute for Regeneration Medicine, Jilin University, Changchun, 130021, China.
authors.forename: Yizhi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Biomedicine, Institute for Regeneration Medicine, Jilin University, Changchun, 130021, China. Electronic address: sundjjl@126.com.
authors.forename: Dejun
authors.initials: D
authors.lastname: Sun
authors.affiliation: Department of Neurology, Brain Hospital of Jilin Province, Siping, 136000, China.
authors.forename: Qingjin
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Department of Neurology, The Second Hospital of Jilin University, Jilin University, Changchun 130041, China.
authors.forename: Wanxu
authors.initials: W
authors.lastname: Guo
authors.affiliation: Department of Neurology, The Second Hospital of Jilin University, Jilin University, Changchun 130041, China.
authors.forename: Qiuhui
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Neurology, The Second Hospital of Jilin University, Jilin University, Changchun 130041, China. Electronic address: m13843440197@163.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000700:Analgesics / Q000008:administration & dosage*; D000818:Animals; D001952:Bridged-Ring Compounds / Q000008:administration & dosage*; D002121:Calcium Channel Blockers / Q000008:administration & dosage; D015220:Calcium Channels / Q000502:physiology*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009553:Nimodipine / Q000008:administration & dosage; D052248:Nitric Oxide Synthase Type I / Q000097:blood; D059225:Nociception / Q000187:drug effects* / Q000502:physiology*; D010147:Pain Measurement; D012701:Serotonin / Q000097:blood; D006027:Glycosides
chemical_list: D000700:Analgesics; D001952:Bridged-Ring Compounds; D002121:Calcium Channel Blockers; D015220:Calcium Channels; D009553:Nimodipine; D052248:Nitric Oxide Synthase Type I; D012701:Serotonin; C014959:albiflorin; D006027:Glycosides
keywords: Albiflorin; Antinociception; Calcium channels; Serotonin