title: Oestrogen receptors beta genotype in infertile Egyptian men with nonobstructive azoospermia.
abstract: In a prospective study, the polymorphism of oestrogen receptor β gene was investigated in nonobstructive azoospermia patients. Ninety infertile patients with nonobstructive azoospermia diagnosed after two semen analysis, 2 weeks apart and negative testicular sperm extraction during intracytoplasmic sperm injection, and 60 fertile men as controls were enrolled in the study. Semen analysis, hormonal profile and allele-specific PCR reaction were performed to detect variants of the RsaI polymorphism of the oestrogen receptor β gene for all patients and controls. The mean patient's age was significantly lower than the mean age of the controls (P < 0.05). There was a significant increase in the mean serum levels of FSH, LH, free testosterone and E2 and significant decrease in total testosterone in patients than controls (P < 0.05). In the patients, the frequency of the homozygous GG, heterozygous AG and homozygous AA genotype was 83.3%, 14.3% and 3.3% respectively, whereas their frequencies in the controls were 95%, 5% and 0% respectively (odds ratio 3.8). There is no significant correlation between ERß polymorphisms and patient's age or pituitary and sex hormones (P > 0.05). Our findings suggested that in Egyptian population, genetic mutation in ERß is associated with the risk of nonobstructive azoospermia.
authors.affiliation: Dermatology and Andrology department, Al-Azhar University, Assiut, Egypt.
authors.forename: A-K H
authors.initials: AH
authors.lastname: Younes
authors.affiliation: Clinical Pathology department, South Egypt Cancer Institute, Assiut University, Assiut, Egypt.
authors.forename: H B
authors.initials: HB
authors.lastname: Hamed
authors.affiliation: Dermatology and Andrology department, Al-Azhar University, Assiut, Egypt.
authors.forename: E-E M
authors.initials: EM
authors.lastname: Mohamed
authors.affiliation: Dermatology and Andrology department, Al-Azhar University, Assiut, Egypt.
authors.forename: M A E
authors.initials: MA
authors.lastname: Makki
authors.affiliation: Clinical Pathology department, South Egypt Cancer Institute, Assiut University, Assiut, Egypt.
authors.forename: N
authors.initials: N
authors.lastname: Gaber
authors.affiliation: Dermatology and Andrology department, Ministry of Health, General Mallawi Hospital, Minea, Egypt.
authors.forename: H M
authors.initials: HM
authors.lastname: Mohamed
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000235:genetics*; D004534:Egypt; D047629:Estrogen Receptor beta / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D020641:Polymorphism, Single Nucleotide; D011446:Prospective Studies; D055815:Young Adult
chemical_list: D047629:Estrogen Receptor beta
keywords: Azoospermia; male infertility; oestrogen receptor