title: Expression of neurotrophic factors in injured spinal cord after transplantation of human-umbilical cord blood stem cells in rats.
abstract: We induced percutaneous spinal cord injuries (SCI) using a balloon catheter in 45 rats and transplanted human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) at the injury site. Locomotor function was significantly improved in hUCB-MSCs transplanted groups. Quantitative ELISA of extract from entire injured spinal cord showed increased expression of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF) and neurotrophin-3 (NT-3). Our results show that treatment of SCI with hUCB-MSCs can improve locomotor functions, and suggest that increased levels of BDNF, NGF and NT-3 in the injured spinal cord were the main therapeutic effect.
authors.affiliation: Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Hyo-jin
authors.initials: HJ
authors.lastname: Chung
authors.affiliation: Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Wook-hun
authors.initials: WH
authors.lastname: Chung
authors.affiliation: Department of Veterinary Surgery, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Dai-Jung
authors.initials: DJ
authors.lastname: Chung
authors.affiliation: Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Wo-Jong
authors.initials: WJ
authors.lastname: Yang
authors.affiliation: Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: A-Jin
authors.initials: AJ
authors.lastname: Lee
authors.affiliation: Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Chi-Bong
authors.initials: CB
authors.lastname: Choi
authors.affiliation: Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Hwa-Seok
authors.initials: HS
authors.lastname: Chang
authors.affiliation: Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Dae-Hyun
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Suh
authors.affiliation: Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Dong-Hun
authors.initials: DH
authors.lastname: Lee
authors.affiliation: Seoul Cord Blood Bank, Histostem Co, Seoul 05372, Korea.
authors.forename: Soo-Han
authors.initials: SH
authors.lastname: Hwang
authors.affiliation: Department of Veterinary Clinical Pathology, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Do
authors.affiliation: Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Hwi-Yool
authors.initials: HY
authors.lastname: Kim
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics*; D036101:Cord Blood Stem Cell Transplantation; D004797:Enzyme-Linked Immunosorbent Assay; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D008124:Locomotion; D020932:Nerve Growth Factor / Q000235:genetics; D051381:Rats; D013119:Spinal Cord Injuries / Q000628:therapy*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D020932:Nerve Growth Factor
keywords: brain-derived neurotrophic factor; nerve growth factor; neurotrophic factor; neurotrophin-3; spinal cord injury