title: Understanding the formation of supported lipid bilayers via vesicle fusion-A case that exemplifies the need for the complementary method approach (Review).
abstract: In this review, the authors discuss the challenges of studying supported lipid bilayers (SLBs) deposited by vesicle fusion in terms of (1) evaluating SLB formation and quality using quartz crystal microbalance with dissipation and (2) analyzing the composition and asymmetry of SLBs composed by lipid mixtures using complementary surface sensitive techniques. An overview of the literature is presented and the inconsistencies on this topic are discussed with the objective to expand beyond simple lipid compositions and set the basis for forming and analyzing SLBs of complex natural lipid extracts formed via the vesicle fusion method. The authors conclude by providing some guidelines to successfully form SLBs of complex lipid mixtures including natural extracts.
authors.affiliation: Nano-Science Center and Department of Chemistry, Copenhagen University, Copenhagen 2010, Malmö, 20506, Denmark.
authors.forename: Tania K
authors.initials: TK
authors.lastname: Lind
authors.affiliation: Nano-Science Center and Department of Chemistry, Copenhagen University, Copenhagen 2010, Malmö, 20506, Denmark and Department of Biomedical Sciences and Biofilm-the Research Center for Biointerfaces, Health & Society, Malmo University, 20500 Malmo, Sweden.
authors.forename: Marité
authors.initials: M
authors.lastname: Cárdenas
mesh_terms: D055592:Biophysical Phenomena; D045424:Complex Mixtures / Q000378:metabolism*; D008051:Lipid Bilayers / Q000378:metabolism*
chemical_list: D045424:Complex Mixtures; D008051:Lipid Bilayers