title: Analysis of amino acid constituents of gallstones.
abstract: OBJECTIVE
  To seek drugs that will efficaciously dissolve bilirubin, glycoprotein and black stones and that will represent improved lithotriptic agents to resolve cholesterol stones, and to study the amino acid constituents of gallstones.
  
  
  METHODS
  According to characteristics determined by infrared spectroscopy and to the contents of bilirubin determined by semi-quantitative chemical analysis, 30 of 148 cases of gallstones were selected and divided into 5 groups. Amino acids of the 30 cases were detected by high-speed chromatography.
  
  
  RESULTS
  The quantity of amino acids was highest in black stones (226.9 mg/g) and lowest in pure cholesterol stones (1.4 mg/g). In the 5 groups of gallstones, the quantity of amino acids followed the hierarchy of black stone > mixed bilirubin stone and glucoprotein stone > mixed cholesterol stone > pure cholesterol stone. The proportions were: 95.95:29.02 and 28.05:5.78:1. Aliphatic amino acids accounted for approximately 50% of the total amino acids in the gallstones, with glycine accounting for 15.3% of the total amount of the 17 kinds of amino acids.
  
  
  CONCLUSIONS
  For mixed stones, the higher level of bilirubin, the higher content of amino acids. Acidic amino acids were relatively higher in bilirubin stones than in cholesterol stones.
authors.affiliation: Ying Chen, Lian-Lian Wang, Department of Gastroenterology, Beijing Hospital, Beijing 100730, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Ying Chen, Lian-Lian Wang, Department of Gastroenterology, Beijing Hospital, Beijing 100730, China.
authors.forename: L L
authors.initials: LL
authors.lastname: Wang
authors.affiliation: Ying Chen, Lian-Lian Wang, Department of Gastroenterology, Beijing Hospital, Beijing 100730, China.
authors.forename: Y X
authors.initials: YX
authors.lastname: Xiao
authors.affiliation: Ying Chen, Lian-Lian Wang, Department of Gastroenterology, Beijing Hospital, Beijing 100730, China.
authors.forename: J H
authors.initials: JH
authors.lastname: Ni
authors.affiliation: Ying Chen, Lian-Lian Wang, Department of Gastroenterology, Beijing Hospital, Beijing 100730, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Yu
keywords: Amino acids/analysis; Bilirubin; Gallstones; Glycine