title: Evaluation of the Luminex xTAG®-GPP (Gastrointestinal Pathogen Panel) in the diagnosis of diseases with acute diarrhoea.
abstract: BACKGROUND
  Most Microbiology laboratories use different techniques for the diagnosis of gastrointestinal infections. Some of which require at least 72 hours to obtain final results.
  
  
  METHODS
  The gastrointestinal panel Luminex (xTAG-GPP, Luminex Molecular Diagnostics, Toronto, Canada) is a qualitative multiplex fast and sensitive assay able to detect and to identify the 15 most common pathogens causing gastrointestinal infection simultaneously. We evaluated this multiplex panel comparing it with conventional methods used in our laboratory.
  
  
  RESULTS
  We analyzed 225 samples of feces. Through the conventional methods were positive 74 samples (32.9%). Through the Luminex method were positive 137 samples (60.9%).
  
  
  CONCLUSIONS
  The use of the xTAG®GPP system in Clinical Microbiology can improve the diagnosis of gastrointestinal infectious because it provides results in less than 8 hours. Some pathogens should be applied with caution and should be interpreted based on the patient́s clinical data.
authors.affiliation: Área Clínica de Microbiología, Enfermedades Infecciosas y Medicina Preventiva, Servicio de Microbiología, Consorcio Hospital General Universitario de Valencia, Valencia, España.
authors.forename: Cristina
authors.initials: C
authors.lastname: Casañ
authors.affiliation: Área Clínica de Microbiología, Enfermedades Infecciosas y Medicina Preventiva, Servicio de Microbiología, Consorcio Hospital General Universitario de Valencia, Valencia, España. Electronic address: ocete_mar@gva.es.
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Ocete
authors.affiliation: Área Clínica de Microbiología, Enfermedades Infecciosas y Medicina Preventiva, Servicio de Microbiología, Consorcio Hospital General Universitario de Valencia, Valencia, España.
authors.forename: Rafael
authors.initials: R
authors.lastname: Medina
authors.affiliation: Área Clínica de Microbiología, Enfermedades Infecciosas y Medicina Preventiva, Servicio de Microbiología, Consorcio Hospital General Universitario de Valencia, Valencia, España.
authors.forename: Concepción
authors.initials: C
authors.lastname: Gimeno
mesh_terms: D000208:Acute Disease; D003967:Diarrhea / Q000209:etiology / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005767:Gastrointestinal Diseases / Q000150:complications / Q000175:diagnosis* / Q000382:microbiology*; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods; D025202:Molecular Diagnostic Techniques / Q000379:methods*
keywords: Diagnóstico molecular; Gastrointestinal infection; Gastrointestinal panel Luminex; Infección gastrointestinal; Molecular diagnosis; Panel gastrointestinal Luminex