title: Decay rates of faecal indicator bacteria from sewage and ovine faeces in brackish and freshwater microcosms with contrasting suspended particulate matter concentrations.
abstract: To safeguard human health, legislative measures require the monitoring of faecal indicator bacteria (FIB) concentrations in recreational and shellfish waters. Consequently, numerous studies have focussed on FIB survival in the water column and more recently in estuarine sediments. However, there is a paucity of information regarding the influence of contrasting suspended particulate matter (SPM) concentrations on the survival of FIB in the water column of estuaries. Here, microcosms containing freshwater or brackish water with low, high and extreme SPM concentrations were inoculated with sewage and ovine faeces and the decay rate of Escherichia coli, coliforms and enterococci were determined by enumeration over five consecutive days. E. coli derived from ovine faeces proliferated and persisted at high levels in both freshwater and brackish microcosms (no decay), whereas ovine enterococci demonstrated a net decay over the duration of the experiment. Furthermore, SPM concentration had a significant effect on the decay rates of both E. coli and enterococci from ovine faeces in brackish microcosms, but decay rate was greater at low SPM concentrations for E. coli, whereas the opposite was observed for enterococci, whose decay rates increased as SPM concentration increased. E. coli, enterococci and coliforms derived from wastewater demonstrated a net decay in both freshwater and brackish microcosms, with contrasting effects of SPM concentration on decay rate. In addition, some FIB groups demonstrated contrasting responses (decay or proliferation) in the first 24h following inoculation into freshwater versus brackish microcosms. Overall, SPM concentrations influenced the proliferation and decay rates of FIB in brackish waters, but had minimal influence in freshwater. These results demonstrate that the survival rates of FIB in aquatic environments are system specific, species and source dependent, and influenced by SPM concentration. This study has important implications for catchment-based risk assessments and source apportionment of FIB pollution in aquatic environments.
authors.affiliation: School of Biological Sciences, Bangor University, Deiniol Road, Bangor, Gwynedd LL57 2UW, UK.
authors.forename: Tracy L
authors.initials: TL
authors.lastname: Perkins
authors.affiliation: School of Ocean Sciences, Bangor University, Deiniol Road, Bangor, Gwynedd LL57 2UW, UK.
authors.forename: Karen
authors.initials: K
authors.lastname: Perrow
authors.affiliation: School of Ocean Sciences, Bangor University, Deiniol Road, Bangor, Gwynedd LL57 2UW, UK.
authors.forename: Paulina
authors.initials: P
authors.lastname: Rajko-Nenow
authors.affiliation: School of Ocean Sciences, Bangor University, Deiniol Road, Bangor, Gwynedd LL57 2UW, UK.
authors.forename: Colin F
authors.initials: CF
authors.lastname: Jago
authors.affiliation: School of Environment, Natural Resources and Geography, Bangor University, Deiniol Road, Bangor, Gwynedd LL57 2UW, UK.
authors.forename: Davey L
authors.initials: DL
authors.lastname: Jones
authors.affiliation: School of Ocean Sciences, Bangor University, Deiniol Road, Bangor, Gwynedd LL57 2UW, UK; Centre for Applied Marine Sciences, Bangor University, Deiniol Road, Bangor, Gwynedd LL57 2UW, UK.
authors.forename: Shelagh K
authors.initials: SK
authors.lastname: Malham
authors.affiliation: School of Biological Sciences, Bangor University, Deiniol Road, Bangor, Gwynedd LL57 2UW, UK. Electronic address: j.mcdonald@bangor.ac.uk.
authors.forename: James E
authors.initials: JE
authors.lastname: McDonald
mesh_terms: D000818:Animals; D004755:Enterobacteriaceae / Q000502:physiology; D016983:Enterococcus / Q000502:physiology; D004926:Escherichia coli / Q000502:physiology; D063366:Estuaries; D005243:Feces / Q000382:microbiology*; D005618:Fresh Water / Q000382:microbiology*; D006801:Humans; D000068357:Saline Waters; D012722:Sewage / Q000382:microbiology*; D012756:Sheep / Q000382:microbiology; D014852:Wales
chemical_list: D012722:Sewage
keywords: Brackish water; Coliforms; Decay rate; E. coli; Enterococci; FIB; Freshwater; Suspended particulate matter