title: Gelatin promotes murine fibrosarcoma L929 cell detachment and protects the cells from TNFα-induced cytotoxicity.
abstract: Gelatin has been considered to exist as intermediate substance of collagen catabolism in tissue remodeling or under inflammatory conditions. We have initiated the study on possible biological functions of gelatin that can exist temporally and locally under the conditions of remodeling and inflammation Materials and methods: To this purpose, we investigated cell proliferation and survival on gelatin-coated dishes and the response to tumor necrosis factor α (TNFα)-induced cytotoxicity in L929 cells. Autophagy level, ATP level, and ROS generation are examined.
  
  
  
  L929 cells detached from the gelatin-coated dishes and formed multicellular aggregates. TNFα-induced cytotoxicity in L929 cells was inhibited by gelatin-coating culture. The cells on gelatin-coated dishes showed reduced cellular ATP levels and increased adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) phosphorylation, leading to increased ROS generation and autophagy.
  
  
  
  This study showed that gelatin-coated culture protected L929 cells from TNFα-induced cytotoxicity and suggested for a possible pathophysiological function of gelatin in regulating cellular functions.
authors.affiliation: a China-Japan Research Institute of Medical and Pharmaceutical Sciences , Shenyang Pharmaceutical University , Shenyang , China.
authors.forename: Hong-Ju
authors.initials: HJ
authors.lastname: Wang
authors.affiliation: a China-Japan Research Institute of Medical and Pharmaceutical Sciences , Shenyang Pharmaceutical University , Shenyang , China.
authors.forename: Meng-Qi
authors.initials: MQ
authors.lastname: Li
authors.affiliation: a China-Japan Research Institute of Medical and Pharmaceutical Sciences , Shenyang Pharmaceutical University , Shenyang , China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: a China-Japan Research Institute of Medical and Pharmaceutical Sciences , Shenyang Pharmaceutical University , Shenyang , China.
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Yao
authors.affiliation: a China-Japan Research Institute of Medical and Pharmaceutical Sciences , Shenyang Pharmaceutical University , Shenyang , China.
authors.forename: Ming-Yu
authors.initials: MY
authors.lastname: Xia
authors.affiliation: a China-Japan Research Institute of Medical and Pharmaceutical Sciences , Shenyang Pharmaceutical University , Shenyang , China.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Hayashi
authors.affiliation: b Nippi Research Institute of Biomatrix, Nippi, Incorporated, Toride , Japan.
authors.forename: Hitomi
authors.initials: H
authors.lastname: Fujisaki
authors.affiliation: b Nippi Research Institute of Biomatrix, Nippi, Incorporated, Toride , Japan.
authors.forename: Shunji
authors.initials: S
authors.lastname: Hattori
authors.affiliation: c Department of Medical Education and Primary Care , Kyoto Prefectural University of Medicine , Kyoto , Japan.
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Tashiro
authors.affiliation: d Department of Clinical and Pharmaceutical Sciences , Showa Pharmaceutical University , Tokyo , Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Onodera
authors.affiliation: a China-Japan Research Institute of Medical and Pharmaceutical Sciences , Shenyang Pharmaceutical University , Shenyang , China.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ikejima
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001343:Autophagy / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002449:Cell Aggregation / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D019610:Cytoprotection / Q000187:drug effects*; D005354:Fibrosarcoma / Q000473:pathology*; D005780:Gelatin / Q000494:pharmacology*; D051379:Mice; D017382:Reactive Oxygen Species / Q000378:metabolism; D034421:Sus scrofa; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D000255:Adenosine Triphosphate; D005780:Gelatin
keywords: Autophagy; TNFα; cell aggregates; detachment; gelatin