title: The expression of osteopontin in breast cancer tissue and its relationship with p21ras and CD44V6 expression.
abstract: OBJECTIVE
  This study aimed to investigate the expression of osteopontin (OPN), p2lras, and CD44V6 in breast cancer tissues, and to analyze the relationships between their expression and a patient's clinicopathological characteristics and five-year survival rate.
  
  
  METHODS
  Streptavidin-peroxidase immunohistochemistry was used to detect the expression of OPN, p2lras, and CD44V6 in tissue samples from 96 breast cancer patients, and the multivariate Cox proportional hazards model (mCOX-PHM) was used to analyze the factors that affect prognosis.
  
  
  RESULTS
  Among the 96 breast cancer patients studied, positive staining for OPN, CD44V6, and p21ras was observed in 54.2%, 58.3%, and 43.8% of samples, respectively. The expression of OPN and CD44V6 were positively correlated (r = 0.58), and the expression of OPN and p21ras were also positively correlated (r = 0.25). Coexpression OPN, CD44V6, and p21ras was negatively correlated with a patient's five-year survival rate (p < 0.05). Kaplan-Meier analysis indicated that a patient without OPN, CD44V6, or p21ras expression had an improved survival (p < 0.05). Results from the mCOX-PHM analysis indicated that CD44V6 expression, the degree of tumor differentiation, and lymph node metastasis were all independent factors that indicate prognosis. The combined detection of OPN, CD44V6, and p21ras could contribute to a more accurate assessment of the biological behavior of breast cancers, and could help to indicate the prognosis of breast cancer patients.
authors.forename: Y L
authors.initials: YL
authors.lastname: Gao
authors.forename: L Q
authors.initials: LQ
authors.lastname: Xing
authors.forename: T J
authors.initials: TJ
authors.lastname: Ren
authors.forename: J F
authors.initials: JF
authors.lastname: Hou
authors.forename: Q
authors.initials: Q
authors.lastname: Xue
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.forename: Y M
authors.initials: YM
authors.lastname: Han
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000737:chemistry* / Q000473:pathology; D005260:Female; D006801:Humans; D018960:Hyaluronan Receptors / Q000032:analysis* / Q000502:physiology; D008875:Middle Aged; D053495:Osteopontin / Q000032:analysis* / Q000502:physiology; D011379:Prognosis; D016016:Proportional Hazards Models; D016283:Proto-Oncogene Proteins p21(ras) / Q000032:analysis* / Q000502:physiology
chemical_list: C112697:CD44v6 antigen; D018960:Hyaluronan Receptors; D053495:Osteopontin; D016283:Proto-Oncogene Proteins p21(ras)