title: Formation of intracellular lumina in human prostate carcinoma (DU145) cells, maturation into signet cells, and the cribriform morphology of tumors.
abstract: The intracellular or intracytoplasmic lumen (IL) is an enigmatic histological structure that occurs in various tumor cells. A reassessment of diverse ILs fine-structure micrographs obtained out of previous studies encompassing the human prostate carcinoma (DU145) cell line and xenotransplanted carcinomas enabled us to propose aspects of ILs development in cancer cells: a combination of altered expressions in intercellular contacts and their cytoskeletal components would favor a disarray of self-apical polarity orientation; those defects, associated with a local, entwined enriched membranous structures growing as microvilli-like formations out of a disrupted endoplasm and trans-Golgi sorting, create ILs in cells' perikarya. These misplaced intracytoplasmic domains can become enlarged through spaces made between the finger-like structures by accruing membranes of coalescent intracytoplasmic vesicles then adding microvilli and glycocalyx to constitute ILs. Cationic mucins added with or without a progressive or total loss of microvilli and content generate signet or ring cell, while ILs enlarge. Variable build-ups of these cells' populations in carcinomas result in architectural mix-up of adjacent cells around these voids, misconstrued as new lumen, and establish a "cribriform" tumor pattern that often implies a poor cancer prognosis. Alternatively, cytotoxic changes caused by anticancer pro-oxidant treatment favor membrane alterations and exaggerate the ILs in xenotransplants into intracellular crypts that accompany other tumor degenerative changes.
authors.affiliation: a Department of Anatomical Sciences , St. George's University International School of Medicine, K. B. Taylor Global Scholar's Programme with Northumbria University , Newcastle upon Tyne , UK.|b Faculté de Médecine , Laboratoire des Cellules et Tissus, Unité de Recherche en Physiologie Moléculaire (URPhyM), University of Namur , Namur , Belgium.
authors.forename: Jacques
authors.initials: J
authors.lastname: Gilloteaux
authors.affiliation: a Department of Anatomical Sciences , St. George's University International School of Medicine, K. B. Taylor Global Scholar's Programme with Northumbria University , Newcastle upon Tyne , UK.
authors.forename: Aneil
authors.initials: A
authors.lastname: Bhalla
authors.affiliation: a Department of Anatomical Sciences , St. George's University International School of Medicine, K. B. Taylor Global Scholar's Programme with Northumbria University , Newcastle upon Tyne , UK.
authors.forename: Omar
authors.initials: O
authors.lastname: Faour
authors.affiliation: c The Apatone Development Center , St. Thomas Hospital, Summa Research Foundation , Akron , OH , USA.
authors.forename: James M
authors.initials: JM
authors.lastname: Jamison
mesh_terms: D000818:Animals; D002277:Carcinoma / Q000473:pathology* / Q000648:ultrastructure*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D064593:Heterografts; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D046529:Microscopy, Electron, Transmission; D011471:Prostatic Neoplasms / Q000473:pathology* / Q000648:ultrastructure*
keywords: Carcinoma; intracellular lumina; prostate; signet cell cribriform; ultrastructure