title: Effect of Hydroalcoholic Extract of Rosmarinus officinalis L. Leaf on Anxiety in Mice.
abstract: In today's stressful world, psychopathy (especially anxiety) is receiving increased importance. Most of the drugs used to treat this disease have several side effects. Medicinal plants derived from natural products have fewer side effects and can be used in the treatment of this disease. The aim of this study was to evaluate the effect of the hydroalcoholic extract of Rosmarinus officinalis L. on anxiety in mice. In this experimental study, 50 male mice were randomly divided into 5 groups. To evaluate anxiety, the Elevated Plus Maze test was performed. The control group received normal saline, the positive control group received diazepam (1 mg/kg) as intraperitoneal injection, and the experimental groups received doses of 100, 200, and 400 mg/kg body weight of rosemary extract. The data were analyzed using SPSS 15 and ANOVA statistical tests. The results show that rosemary extract dose-dependently increases the mice spending time and the entries number of mice in plus maze open arms (indicating less stress). This effect at a dose of 400 mg/kg was similar to diazepam, which, in comparison to the control group, was statistically significant (P < .01), while the evaluation of locomotor activity in treated groups, compared with the control groups, showed no significant difference (P > .05). On the other hand, the rosemary extract, similar to the standard drug diazepam, showed an anti-anxiety effect. This effect is probably due to the presence of flavonoids in this plant and their antioxidant property.
authors.affiliation: School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.
authors.forename: Mosayeb
authors.initials: M
authors.lastname: Noori Ahmad Abadi
authors.affiliation: School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Mortazavi
authors.affiliation: Medical Ethics Research Center, Jahrom University of Medical Sciences, Jahrom, Iran.
authors.forename: Navid
authors.initials: N
authors.lastname: Kalani
authors.affiliation: Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Hadi Zare
authors.initials: HZ
authors.lastname: Marzouni
authors.affiliation: Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran wesamkooti@gmail.com.
authors.forename: Wesam
authors.initials: W
authors.lastname: Kooti
authors.affiliation: Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran.
authors.forename: Sara
authors.initials: S
authors.lastname: Ali-Akbari
mesh_terms: D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001007:Anxiety / Q000188:drug therapy*; D001522:Behavior, Animal / Q000187:drug effects; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry*; D027542:Rosmarinus / Q000737:chemistry*
chemical_list: D014151:Anti-Anxiety Agents; D010936:Plant Extracts
keywords: Rosmarinus officinalis; anxiolytic; mice