title: Knockdown of SCARA5 inhibits PDGF-BB-induced vascular smooth muscle cell proliferation and migration through suppression of the PDGF signaling pathway.
abstract: Vascular smooth muscle cell (VSMC) proliferation and migration are critical in the progression of atherosclerosis and can be induced by platelet-derived growth factor (PDGF). Several studies have demonstrated that scavenger receptor class A, member 5 (SCARA5) is important in cancer cell migration and invasion. However, the role of SCARA5 in VSMCs remains to be elucidated in the development of atherosclerosis. Therefore, the role of SCARA5 was investigated in PDGF‑BB‑stimulated VSMC proliferation and migration. In the present study, it was shown that SCARA5 expression was enhanced by PDGF‑BB in human aortic smooth muscle cells (HASMCs). Knockdown of SCARA5 by small interfering (si)RNA significantly inhibited PDGF‑BB‑induced HASMC proliferation and migration. Furthermore, siRNA‑SCARA5 significantly inhibited the phosphorylation of PDGF receptor (PDGFR) β, AKT and extracellular signal‑regulated kinase 1/2 in PDGF‑BB‑stimulated HASMCs. In conclusion, this study demonstrated that knockdown of SCARA5 inhibits PDGF‑BB‑induced HASMC proliferation and migration through suppression of the PDGF signaling pathway. Thus, SCARA5 may be a novel therapeutic target for preventing or treating vascular diseases involving VSMC proliferation and migration.
authors.affiliation: The Second Ward of Cardiovascular Department, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China.
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Zhao
authors.affiliation: The Second Ward of Cardiovascular Department, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China.
authors.forename: Liguo
authors.initials: L
authors.lastname: Jian
authors.affiliation: The Second Ward of Cardiovascular Department, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhang
authors.affiliation: The Second Ward of Cardiovascular Department, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China.
authors.forename: Tongbin
authors.initials: T
authors.lastname: Ding
authors.affiliation: The Second Ward of Cardiovascular Department, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Li
authors.affiliation: The Second Ward of Cardiovascular Department, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China.
authors.forename: Dong
authors.initials: D
authors.lastname: Cheng
authors.affiliation: The Second Ward of Cardiovascular Department, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China.
authors.forename: Shaohui
authors.initials: S
authors.lastname: Niu
authors.affiliation: The Second Ward of Cardiovascular Department, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China.
authors.forename: Liqiang
authors.initials: L
authors.lastname: Sun
authors.affiliation: The Second Ward of Cardiovascular Department, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China.
authors.forename: En
authors.initials: E
authors.lastname: Li
authors.affiliation: The Second Ward of Cardiovascular Department, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China.
authors.forename: Shichao
authors.initials: S
authors.lastname: Liu
authors.affiliation: The Second Ward of Cardiovascular Department, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China.
authors.forename: Youxu
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Nephrology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China.
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
mesh_terms: D000077214:Becaplermin; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects* / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects* / Q000235:genetics; D055785:Gene Knockdown Techniques; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects* / Q000235:genetics; D009131:Muscle, Smooth, Vascular / Q000378:metabolism* / Q000473:pathology; D032389:Myocytes, Smooth Muscle / Q000378:metabolism* / Q000473:pathology; D020574:Proto-Oncogene Proteins c-sis / Q000494:pharmacology*; D051117:Scavenger Receptors, Class A / Q000235:genetics / Q000378:metabolism*; D014652:Vascular Diseases / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy
chemical_list: D020574:Proto-Oncogene Proteins c-sis; C569833:SCARA5 protein, human; D051117:Scavenger Receptors, Class A; D000077214:Becaplermin