title: Fermentative utilization of coffee mucilage using Bacillus coagulans and investigation of down-stream processing of fermentation broth for optically pure l(+)-lactic acid production.
abstract: In this study, mucilage, a residue from coffee production, was investigated as substrate in fermentative l(+)-lactic acid production. Mucilage was provided as liquid suspension consisting glucose, galactose, fructose, xylose and sucrose as free sugars (up to 60gL(-1)), and used directly as medium in Bacillus coagulans batch fermentations carried out at 2 and 50L scales. Using mucilage and 5gL(-1) yeast extract as additional nitrogen source, more than 40gL(-1) lactic acid was obtained. Productivity and yield were 4-5gL(-1)h(-1) and 0.70-0.77g lactic acid per g of free sugars, respectively, irrespective the scale. Similar yield was found when no yeast extract was supplied, the productivity, however, was 1.5gL(-1)h(-1). Down-stream processing of culture broth, including filtration, electrodialysis, ion exchange chromatography and distillation, resulted in a pure lactic acid formulation containing 930gL(-1)l(+)-lactic acid. Optical purity was 99.8%.
authors.affiliation: Leibniz Institute for Agricultural Engineering Potsdam-Bornim, Max-Eyth-Allee 100, 14469 Potsdam, Germany.
authors.forename: Anna-Katrin
authors.initials: AK
authors.lastname: Neu
authors.affiliation: Leibniz Institute for Agricultural Engineering Potsdam-Bornim, Max-Eyth-Allee 100, 14469 Potsdam, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Pleissner
authors.affiliation: Leibniz Institute for Agricultural Engineering Potsdam-Bornim, Max-Eyth-Allee 100, 14469 Potsdam, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Mehlmann
authors.affiliation: Leibniz Institute for Agricultural Engineering Potsdam-Bornim, Max-Eyth-Allee 100, 14469 Potsdam, Germany.
authors.forename: Roland
authors.initials: R
authors.lastname: Schneider
authors.affiliation: Cenicafé, National Coffee Research Center, Sede Planalto, km. 4 via Chinchiná-Manizales, Manizales (Caldas), Colombia.
authors.forename: Gloria Inés
authors.initials: GI
authors.lastname: Puerta-Quintero
authors.affiliation: Leibniz Institute for Agricultural Engineering Potsdam-Bornim, Max-Eyth-Allee 100, 14469 Potsdam, Germany. Electronic address: jvenus@atb-potsdam.de.
authors.forename: Joachim
authors.initials: J
authors.lastname: Venus
mesh_terms: D000070000:Bacillus coagulans / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D003069:Coffee; D005285:Fermentation; D019344:Lactic Acid / Q000096:biosynthesis*; D009584:Nitrogen
chemical_list: D003069:Coffee; D019344:Lactic Acid; D009584:Nitrogen
keywords: Bacillus coagulans; Coffee mucilage; Nutrient recovery; Renewable resources; Waste utilization