title: Three-step procedure for preparation of pure Bacillus altitudinis ribonuclease.
abstract: Ribonucleases are considered as promising tools for anticancer treatment due to their selective cytotoxicity against tumor cells. We investigated a new RNase from Bacillus altitudinis termed BALNASE (B. altitudinis RNase). Balnase is a close homolog of the well-known cytotoxic binase, differing by only one amino acid residue: nonpolar hydrophobic alanine at position 106 in the balnase molecule is replaced by a polar uncharged threonine in binase. The most exciting question is how the physico-chemical properties and biological effects of RNase might be changed by A106T substitution. Here, we have developed a chromatography-based rapid and modern technique for the purification of this new RNase which allowed us to get a protein sample of high quality with specific activity of 1.2 × 10(6) units in preparative amounts, suitable for further investigation of its biological properties.
authors.affiliation: Institute of Fundamental Medicine and Biology Kazan Federal (Volga-Region) University Russia.
authors.forename: Elena
authors.initials: E
authors.lastname: Dudkina
authors.affiliation: Institute of Fundamental Medicine and Biology Kazan Federal (Volga-Region) University Russia.
authors.forename: Vera
authors.initials: V
authors.lastname: Ulyanova
authors.affiliation: Institute of Fundamental Medicine and Biology Kazan Federal (Volga-Region) University Russia.
authors.forename: Raihan
authors.initials: R
authors.lastname: Shah Mahmud
authors.affiliation: Institute of Fundamental Medicine and Biology Kazan Federal (Volga-Region) University Russia.
authors.forename: Vera
authors.initials: V
authors.lastname: Khodzhaeva
authors.affiliation: Institute of Fundamental Medicine and Biology Kazan Federal (Volga-Region) University Russia.
authors.forename: Linh
authors.initials: L
authors.lastname: Dao
authors.affiliation: Institute of Fundamental Medicine and Biology Kazan Federal (Volga-Region) University Russia.
authors.forename: Valentina
authors.initials: V
authors.lastname: Vershinina
authors.affiliation: Institute of Fundamental Medicine and Biology Kazan Federal (Volga-Region) University Russia.
authors.forename: Alexei
authors.initials: A
authors.lastname: Kolpakov
authors.affiliation: Institute of Fundamental Medicine and Biology Kazan Federal (Volga-Region) University Russia.
authors.forename: Olga
authors.initials: O
authors.lastname: Ilinskaya
keywords: Bacillus altitudinis; balnase; binase; homogeneous; purification; ribonuclease; substitution