title: Synthesis, characterization, DNA/protein interaction and cytotoxicity studies of Cu(II) and Co(II) complexes derived from dipyridyl triazole ligands.
abstract: Four different transition metal complexes containing dipyridyl triazole ligands, namely [Cu(abpt)2Cl2]·2H2O (1), [Cu(abpt)2(ClO4)2] (2), [Co2(abpt)2(H2O)2Cl2]·Cl2·4H2O (3) and [Co2(Hbpt)2(CH3OH)2(NO3)2] (4) have been designed, synthesized and further structurally characterized by X-ray crystallography, ESI-MS, elemental analysis, IR and Raman spectroscopy. In these complexes, the both ligands act as bidentate ligands with N, N donors. DNA binding interactions with calf thymus DNA (ct-DNA) of the ligand and its complexes 1~4 were investigated via electronic absorption, fluorescence quenching, circular dichroism and viscosity measurements as well as confocal Laser Raman spectroscopy. The results show these complexes are able to bind to DNA via the non-covalent mode i.e. intercalation and groove binding or electrostatic interactions. The interactions with bovine serum albumin (BSA) were also studied using UV-Vis and fluorescence spectroscopic methods which indicated that fluorescence quenching of BSA by these compounds was the presence of both static and dynamic quenching. Moreover, the in vitro cytotoxic effects of the complexes against four cell lines SK-OV-3, HL-7702, BEL7404 and NCI-H460 showed the necessity of the coordination action on the biological properties on the respective complex and that all four complexes exhibited substantial cytotoxic activity.
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmacy, Guangxi Normal University, Guilin 541004, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmacy, Guangxi Normal University, Guilin 541004, PR China. Electronic address: petto19@163.com.
authors.forename: Di
authors.initials: D
authors.lastname: Yao
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmacy, Guangxi Normal University, Guilin 541004, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmacy, Guangxi Normal University, Guilin 541004, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Tang
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmacy, Guangxi Normal University, Guilin 541004, PR China; School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Key Laboratory of Chemistry and Engineering of Forest Products, Nanning 530008, PR China.
authors.forename: He-Dong
authors.initials: HD
authors.lastname: Bian
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmacy, Guangxi Normal University, Guilin 541004, PR China. Electronic address: huangfp2010@163.com.
authors.forename: Fu-Ping
authors.initials: FP
authors.lastname: Huang
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmacy, Guangxi Normal University, Guilin 541004, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liang
mesh_terms: D015082:2,2'-Dipyridyl / Q000138:chemical synthesis* / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002942:Circular Dichroism; D003035:Cobalt / Q000494:pharmacology*; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D003300:Copper / Q000494:pharmacology*; D018360:Crystallography, X-Ray; D004247:DNA / Q000378:metabolism*; D004996:Ethidium / Q000378:metabolism; D006801:Humans; D020128:Inhibitory Concentration 50; D008024:Ligands; D008968:Molecular Conformation; D011485:Protein Binding / Q000187:drug effects; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013059:Spectrum Analysis, Raman; D013816:Thermodynamics; D014230:Triazoles / Q000138:chemical synthesis* / Q000494:pharmacology; D014783:Viscosity
chemical_list: D056831:Coordination Complexes; D008024:Ligands; D014230:Triazoles; D012710:Serum Albumin, Bovine; D003035:Cobalt; D015082:2,2'-Dipyridyl; D003300:Copper; D004247:DNA; C427203:calf thymus DNA; D004996:Ethidium
keywords: BSA; Cytotoxic activity; DNA; Dipyridyl triazole ligands