title: Visualizing the Functional Heterogeneity of Muscle Stem Cells.
abstract: Skeletal muscle stem cells are satellite cells that play crucial roles in tissue repair and regeneration after muscle injury. Accumulating evidence indicates that satellite cells are genetically and functionally heterogeneous, even within the same muscle. A small population of satellite cells possesses "stemness" and exhibits the remarkable ability to regenerate through robust self-renewal when transplanted into a regenerating muscle niche. In contrast, not all satellite cells self-renew. For example, some cells are committed myogenic progenitors that immediately undergo myogenic differentiation with minimal cell division after activation. Recent studies illuminate the cellular and molecular characteristics of the functional heterogeneity among satellite cells. To evaluate heterogeneity and stem cell dynamics, here we describe methods to conduct a clonal analysis of satellite cells and to visualize a slowly dividing cell population.
authors.affiliation: Department of Stem Cell Biology, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, 852-8523, Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Kitajima
authors.affiliation: Department of Stem Cell Biology, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, 852-8523, Japan.
authors.forename: Shizuka
authors.initials: S
authors.lastname: Ogawa
authors.affiliation: Department of Stem Cell Biology, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, 852-8523, Japan. yusuke-ono@nagasaki-u.ac.jp.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Ono
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D058948:Cell Tracking / Q000379:methods*; D018740:Genetic Heterogeneity; D051379:Mice; D024510:Muscle Development / Q000235:genetics; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology*; D059010:Single-Cell Analysis / Q000379:methods*; D013234:Stem Cells / Q000166:cytology*
keywords: Clonal analysis; Functional heterogeneity; Muscle stem cells; Satellite cells; Single cell analysis; Slow-dividing cells