title: Effect of green tea catechins on gastric mucosal dysplasia in insulin-gastrin mice.
abstract: Green tea catechins (GTCs) have been implicated in various physiological effects, including anti-carcinogenic activities. In the present study, we evaluated the effects of GTCs specifically on the development of gastritis and pre-malignant lesions in insulin-gastrin mice. Nine-week-old male INS-GAS mice (n=38) were supplemented with GTCs for 4 and 28 weeks, and their body weights, serum gastrin levels, histopathology and pro-inflammatory cytokine levels in gastric tissue and mucosal cell proliferation were monitored. Body weights of the GTC-treated mice were significantly lower than those of the untreated controls (P≤0.05). Serum gastrin levels were suppressed at the age of 37-weeks (P≤0.05). The histopathological scores indicated that the extent of dysplasia was significantly diminished (P≤0.05), although GTC supplementation did not affect the inflammation scores. The messenger RNA levels of interferon (IFN)-γ were significantly reduced at the age of 13 weeks (P≤0.05), although the changes did not reach statistical significance at the age of 37 weeks (P=0.056). The labeling index of Ki-67 immunohistochemistry was significantly decreased (P≤0.05). These results demonstrated that GTCs may play a protective role in the development of gastritis and pre-malignant lesions via an IFN-γ, gastrin, and mucosal cell proliferation-dependent mechanism in this rodent model and potentially in humans.
authors.affiliation: Second Department of Internal Medicine, Faculty of Medical Sciences, University of Fukui, Eiheiji-cho, Yoshida-gun, Fukui 910-1193, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ohno
authors.affiliation: Second Department of Internal Medicine, Faculty of Medical Sciences, University of Fukui, Eiheiji-cho, Yoshida-gun, Fukui 910-1193, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ohtani
authors.affiliation: Second Department of Internal Medicine, Faculty of Medical Sciences, University of Fukui, Eiheiji-cho, Yoshida-gun, Fukui 910-1193, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Suto
authors.affiliation: Division of Surgical Pathology, University of Fukui Hospital, Fukui, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Ohta
authors.affiliation: Division of Surgical Pathology, University of Fukui Hospital, Fukui, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Imamura
authors.affiliation: Second Department of Internal Medicine, Faculty of Medical Sciences, University of Fukui, Eiheiji-cho, Yoshida-gun, Fukui 910-1193, Japan.
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Matsuda
authors.affiliation: Second Department of Internal Medicine, Faculty of Medical Sciences, University of Fukui, Eiheiji-cho, Yoshida-gun, Fukui 910-1193, Japan.
authors.forename: Katsushi
authors.initials: K
authors.lastname: Hiramatsu
authors.affiliation: Second Department of Internal Medicine, Faculty of Medical Sciences, University of Fukui, Eiheiji-cho, Yoshida-gun, Fukui 910-1193, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Nemoto
authors.affiliation: Second Department of Internal Medicine, Faculty of Medical Sciences, University of Fukui, Eiheiji-cho, Yoshida-gun, Fukui 910-1193, Japan.
authors.forename: Yasunari
authors.initials: Y
authors.lastname: Nakamoto
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D028241:Camellia sinensis / Q000737:chemistry*; D002392:Catechin / Q000494:pharmacology*; D049109:Cell Proliferation; D004353:Drug Evaluation, Preclinical; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005755:Gastrins / Q000097:blood; D005756:Gastritis / Q000188:drug therapy*; D007371:Interferon-gamma / Q000096:biosynthesis; D008297:Male; D051379:Mice; D013274:Stomach Neoplasms / Q000517:prevention & control
chemical_list: D016588:Anticarcinogenic Agents; D005755:Gastrins; D007371:Interferon-gamma; D002392:Catechin