title: Optimal sequence of antisense DNA to silence YB-1 in lung cancer by use of a novel polysaccharide drug delivery system.
abstract: Silencing Y-box binding protein 1 (YB-1) can be an excellent target for cancer therapy and many lung cancer cells express the polysaccharide-recognition receptor Dectin-1. We designed a Dectin-1 targeting vehicle delivering YB-1-antisense DNA. First, we selected five optimal antisense DNA sequences to silence YB-1 from among 153 candidates. We chose the sequence closest to the start codon (AS014), and attached dA40 to the 3' end; dA40 promotes complex formation with a β-(1➝3)-d-glucan called schizophyllan (SPG). The resultant complexes were applied to 12 human-oriented lung cancer cell lines, and cell viability was examined. The cell lines exhibited decreased viability and showed strong affinity to bind SPG, suggesting the AS014/SPG complex entered the cells via the Dectin-1 mediated pathway.
authors.affiliation: University of Occupational and Environmental Health, Yahatanishi-ku, Kitakyushu, Fukuoka 807-8555, Japan.
authors.forename: Hiroto
authors.initials: H
authors.lastname: Izumi
authors.affiliation: Department of Chemistry and Biochemistry, University of Kitakyushu, Wakamatsu-ku, Kitakyushu, Fukuoka 808-0135, Japan.
authors.forename: Shohei
authors.initials: S
authors.lastname: Nagao
authors.affiliation: Department of Chemistry and Biochemistry, University of Kitakyushu, Wakamatsu-ku, Kitakyushu, Fukuoka 808-0135, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Mochizuki
authors.affiliation: Department of Chemistry and Biochemistry, University of Kitakyushu, Wakamatsu-ku, Kitakyushu, Fukuoka 808-0135, Japan.
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Fujiwara
authors.affiliation: Department of Chemistry and Biochemistry, University of Kitakyushu, Wakamatsu-ku, Kitakyushu, Fukuoka 808-0135, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sakurai
authors.affiliation: University of Occupational and Environmental Health, Yahatanishi-ku, Kitakyushu, Fukuoka 807-8555, Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Morimoto
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D016373:DNA, Antisense / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D016503:Drug Delivery Systems; D020868:Gene Silencing; D006801:Humans; D037181:Lectins, C-Type / Q000737:chemistry*; D008175:Lung Neoplasms / Q000235:genetics*; D012566:Sizofiran / Q000737:chemistry*; D051840:Y-Box-Binding Protein 1 / Q000037:antagonists & inhibitors / Q000235:genetics*; D000099181:Dectin-1
chemical_list: D016373:DNA, Antisense; D037181:Lectins, C-Type; D012566:Sizofiran; D051840:Y-Box-Binding Protein 1; D000099181:Dectin-1; C070856:YBX1 protein, human