title: MicroRNA-194 restrains the cell progression of non-small cell lung cancer by targeting human nuclear distribution protein C.
abstract: NSCLC accounts for over 80% of all lung cancers and is associated with poor prognosis. Human nuclear distribution C (hNUDC) was predicted to be the target gene of microRNA-194 (miR-194). The present study was designed to demonstrate the mechanism of miR-194 in the regulation of non-small cell lung cancer (NSCLC) via targeting the hNUDC. The hNUDC expression was found to strongly be increased while the miR-194 decreased significantly in the NSCLC cell lines when compared with the healthy controls. Moreover, the luciferase report confirmed the targeting reaction between miR-194 and hNUDC. After transfection with miR-194 mimic into NSCLC cells, we found that the miR-194 overexpression resulted in abnormal nuclear division, decreased cell proliferation and inhibited the expression of hNUDC and Mpl/ERK pathway proteins. Furthermore, the hNUDC overexpression affected the suppression effect of miR-194 in 95D cells, indicating that miR-194 suppresses tumor cell process by inhibiting the hNUDC expression. In brief, the present study suggests that the upregulation of miR-194 affects the hNUDC expression, leading to a downregulated expression of Mpl/ERK pathway proteins, and suppresses the mitosis and proliferation of NSCLC cells. These results offer a potential therapeutic strategy for the treatment of lung cancer.
authors.affiliation: Respiratory Department, Cangzhou Central Hospital, Cangzhou, Hebei 061001, P.R. China.
authors.forename: Lirong
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Respiratory Department, Cangzhou Central Hospital, Cangzhou, Hebei 061001, P.R. China.
authors.forename: Qingguo
authors.initials: Q
authors.lastname: Di
authors.affiliation: Respiratory Department, Cangzhou Central Hospital, Cangzhou, Hebei 061001, P.R. China.
authors.forename: Baohua
authors.initials: B
authors.lastname: Sun
authors.affiliation: Respiratory Department, Cangzhou Central Hospital, Cangzhou, Hebei 061001, P.R. China.
authors.forename: Xiaosheng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Respiratory Department, Cangzhou Central Hospital, Cangzhou, Hebei 061001, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: Respiratory Department, Cangzhou Central Hospital, Cangzhou, Hebei 061001, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Shi
mesh_terms: D000818:Animals; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology* / Q000628:therapy; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology* / Q000628:therapy; D020935:MAP Kinase Signaling System; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000502:physiology*; D009687:Nuclear Proteins / Q000235:genetics*; D053628:Receptors, Thrombopoietin / Q000037:antagonists & inhibitors / Q000502:physiology
chemical_list: D018797:Cell Cycle Proteins; C554846:MIRN194 microRNA, human; D035683:MicroRNAs; C512648:NUDC protein, human; D009687:Nuclear Proteins; D053628:Receptors, Thrombopoietin; C082218:MPL protein, human