title: Fluorescence resonance energy transfer between bovine serum albumin and fluoresceinamine.
abstract: Physical binding-mediated organic dye direct-labelling of proteins could be a promising technology for bio-nanomedical applications. Upon binding, it was found that fluorescence resonance energy transfer (FRET) occurred between donor bovine serum albumin (BSA; an amphiphilic protein) and acceptor fluoresceinamine (FA; a hydrophobic fluorophore), which could explain fluorescence quenching found for BSA. FRET efficiency and the distance between FA and BSA tryptophan residues were determined to 17% and 2.29 nm, respectively. Using a spectroscopic superimposition method, the saturated number of FAs that bound to BSA was determined as eight to give a complex formula of FA8-BSA. Finally, molecular docking between BSA and FA was conducted, and conformational change that occurred in BSA upon binding to FA molecules was also studied by three-dimensional fluorescence microscopy.
authors.affiliation: Agricultural Nanocenter, School of Life Sciences, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Hohhot, 010018, China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Bai
authors.affiliation: Agricultural Nanocenter, School of Life Sciences, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Hohhot, 010018, China.
authors.forename: Yushuang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Agricultural Nanocenter, School of Life Sciences, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Hohhot, 010018, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Agricultural Nanocenter, School of Life Sciences, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Hohhot, 010018, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Guo
authors.affiliation: Agricultural Nanocenter, School of Life Sciences, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Hohhot, 010018, China.
authors.forename: Yuxing
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Agricultural Nanocenter, School of Life Sciences, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Hohhot, 010018, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Yun
authors.affiliation: Agricultural Nanocenter, School of Life Sciences, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Hohhot, 010018, China.
authors.forename: Xinmin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Agricultural Nanocenter, School of Life Sciences, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Hohhot, 010018, China.
authors.forename: Ruibo
authors.initials: R
authors.lastname: Zhong
authors.affiliation: Agricultural Nanocenter, School of Life Sciences, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Hohhot, 010018, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002417:Cattle; D005452:Fluoresceins / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D005452:Fluoresceins; D012710:Serum Albumin, Bovine; C032376:fluoresceinamine isomer II
keywords: binding sites; bovine serum albumin; fluoresceinamine; fluorescence resonance energy transfer; three-dimensional fluorescence microscopy