title: Cell wall glycopolymers of Streptomyces albus, Streptomyces albidoflavus and Streptomyces pathocidini.
abstract: The cell wall glycopolymers of three strains of Streptomyces albus and the type strain of Streptomyces pathocidini were investigated. The structures of the glycopolymers were established using a combination of chemical and NMR spectroscopic methods. The cell wall of S. albus subsp. albus VKM Ac-35(T) was found to be comprised of three glycopolymers, viz. unsubstituted 1,5-poly(ribitol phosphate), 1,3-poly(glycerol phosphate) substituted with β-D-glucopyranose, and the major polymer, a 3-deoxy-D-glycero-D-galacto-non-2-ulosonic acid (Kdn)-teichulosonic acid: β-D-Glcp-(1 → 8)-α-Kdnp-(2[(→6)-β-D-Glcp-(1 → 8)-α-Kdnp-(2 →] n 6)-β-D-Glcp-(1 → 8)-β-Kdnp-(2-OH, where n ≥ 3. The cell walls of 'S. albus' J1074 and 'S. albus' R1-100 were found to contain three glycopolymers of identical structures, viz. unsubstituted 1,3- and 2,3-poly(glycerol phosphates), and the major polymer, a Kdn-teichulosonic acid with an unusual structure that has not been previously described: β-D-Galp-(1 → 9)-α-Kdnp-(2[(→3)-β-D-Galp-(1 → 9)-α-Kdnp-(2 →] n 3)-β-D-Galp-(1 → 9)-β-Kdnp-(2-OH, where n ~ 7-8. The cell wall of S. pathocidini (formerly S. albus subsp. pathocidicus) VKM Ac-598(T) was found to contain two glycopolymers, viz. 1,3-poly(glycerol phosphate) partially O-glycosylated with 2-acetamido-2-deoxy-α-D-glucopyranose and/or O-acylated with L-lysine, and a poly(diglycosyl 1-phosphate) of hitherto unknown structure: -6)-α-D-Glcp-(1 → 6)-α-D-GlcpNAc-(1-P-.
authors.affiliation: ND Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect, 47, Moscow, Russia, 119991.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.affiliation: School of Biology MV, Lomonosov Moscow State University, Vorob'evy gory 1 Stroenie 12, Moscow, Russia, 119991. streshinskaya@mail.ru.
authors.forename: Galina M
authors.initials: GM
authors.lastname: Streshinskaya
authors.affiliation: School of Biology MV, Lomonosov Moscow State University, Vorob'evy gory 1 Stroenie 12, Moscow, Russia, 119991.
authors.forename: Elena M
authors.initials: EM
authors.lastname: Tul'skaya
authors.affiliation: ND Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect, 47, Moscow, Russia, 119991.
authors.forename: Sophia N
authors.initials: SN
authors.lastname: Senchenkova
authors.affiliation: All-Russian Collection of Microorganisms (VKM), G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, Russia, 142290.
authors.forename: Lidia M
authors.initials: LM
authors.lastname: Baryshnikova
authors.affiliation: ND Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect, 47, Moscow, Russia, 119991.
authors.forename: Andrey S
authors.initials: AS
authors.lastname: Dmitrenok
authors.affiliation: Department of Genetics and Biotechnology, Ivan Franko National University of Lviv, Lviv, 79005, Ukraine.
authors.forename: Bohdan E
authors.initials: BE
authors.lastname: Ostash
authors.affiliation: Department of Genetics and Biotechnology, Ivan Franko National University of Lviv, Lviv, 79005, Ukraine.
authors.forename: Victor A
authors.initials: VA
authors.lastname: Fedorenko
mesh_terms: D002473:Cell Wall / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010710:Phosphates / Q000302:isolation & purification / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification / Q000378:metabolism*; D013302:Streptomyces / Q000737:chemistry / Q000378:metabolism*; D013400:Sugar Acids / Q000302:isolation & purification / Q000378:metabolism; D013682:Teichoic Acids / Q000302:isolation & purification / Q000378:metabolism; D014574:Uronic Acids / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D010710:Phosphates; D011135:Polysaccharides, Bacterial; D013400:Sugar Acids; D013682:Teichoic Acids; D014574:Uronic Acids; C010788:teichuronic acid; D008239:Lysine
keywords: Cell wall; NMR-spectroscopy; Poly(glycosyl 1-phosphate); Streptomyces; Teichoic acid; Teichulosonic acid