title: Molecular cloning, purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein.
abstract: The Brucella mdh gene was successfully cloned and expressed in E. coli. The purified recombinant malate dehydrogenase protein (rMDH) was reactive to Brucella-positive bovine serum in the early stage, but not reactive in the middle or late stage, and was reactive to Brucella-positive mouse serum in the late stage, but not in the early or middle stage of infection. In addition, rMDH did not react with Brucella-negative bovine or mouse sera. These results suggest that rMDH has the potential for use as a specific antigen in serological diagnosis for early detection of bovine brucellosis.
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.; Department of Veterinary Paraclinical Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, College, Laguna 4031, Philippines.
authors.forename: Alisha Wehdnesday Bernardo
authors.initials: AW
authors.lastname: Reyes
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Hannah Leah Tadeja
authors.initials: HL
authors.lastname: Simborio
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Huynh Tan
authors.initials: HT
authors.lastname: Hop
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Lauren Togonon
authors.initials: LT
authors.lastname: Arayan
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.; Institute of Agriculture and Life Science, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Suk
authors.initials: S
authors.lastname: Kim
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D002003:Brucella abortus / Q000201:enzymology* / Q000276:immunology; D002006:Brucellosis / Q000175:diagnosis / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics; D008291:Malate Dehydrogenase / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D011994:Recombinant Proteins; D008291:Malate Dehydrogenase
keywords: Brucella; expression; immunogenicity; malate dehydrogenase; recombinant protein