title: A FRET-enabled molecular peptide beacon with a significant red shift for the ratiometric detection of nucleic acids.
abstract: A cationic molecular peptide beacon NAP1 functionalized with a fluorescence resonance energy transfer-pair at its ends allows the ratiometric detection of ds-DNA with a preference for AT rich sequences. NAP1 most likely binds in a folded form into the minor groove of ds-DNA, which results in a remarkable change in its fluorescence properties. As NAP1 exhibits quite low cytotoxicity, it can also be used for imaging of nuclear DNA in cells.
authors.affiliation: Institute for Organic Chemistry, University of Duisburg-Essen, 45117, Essen, Germany. carsten.schmuck@uni-due.de.
authors.forename: Debabrata
authors.initials: D
authors.lastname: Maity
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Jiang
authors.forename: Martin
authors.initials: M
authors.lastname: Ehlers
authors.forename: Junchen
authors.initials: J
authors.lastname: Wu
authors.forename: Carsten
authors.initials: C
authors.lastname: Schmuck
mesh_terms: D000072283:A549 Cells; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000032:analysis*; D031541:Fluorescence Resonance Energy Transfer; D018613:Microscopy, Confocal; D009281:Naphthalenes / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D009841:Oligonucleotides / Q000737:chemistry; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D011721:Pyrenes / Q000737:chemistry
chemical_list: D009281:Naphthalenes; D009841:Oligonucleotides; D009842:Oligopeptides; D010455:Peptides; D011721:Pyrenes; C477371:locked nucleic acid; C000624253:nucleic acid probe 1; D004247:DNA; C427203:calf thymus DNA; C030984:pyrene