title: Purification of Virus-Like Particles (VLPs) from Plants.
abstract: Viral coat proteins expressed in plants often form virus-like particles (VLPs) which are good vaccine candidates as they are safe and highly immunogenic and can be easily purified. The VLPs can be purified by rate-zonal density centrifugation which is based on the size of the VLP or they can be purified by isopycnic centrifugation which is a fast and simple method and results in isolation of VLPs with the same density. Details on how to apply both rate-zonal and isopycnic centrifugation for VLP purification from plants are provided in this chapter.
authors.affiliation: Biopharming Research Unit, Department of Molecular and Cell Biology, University of Cape Town, Private Bag, Rondebosch, 7700, South Africa.
authors.forename: Albertha R
authors.initials: AR
authors.lastname: van Zyl
authors.affiliation: Biopharming Research Unit, Department of Molecular and Cell Biology, University of Cape Town, Private Bag, Rondebosch, 7700, South Africa. inga.hitzeroth@uct.ac.za.
authors.forename: Inga I
authors.initials: II
authors.lastname: Hitzeroth
mesh_terms: D036022:Capsid Proteins / Q000235:genetics* / Q000302:isolation & purification*; D002498:Centrifugation / Q000379:methods*; D010944:Plants / Q000235:genetics*
chemical_list: D036022:Capsid Proteins
keywords: Centrifugation; Isopycnic; Plants; Rate-zonal; VLPs; Virus-like particles