title: Cytokine-like 1 Chemoattracts Monocytes/Macrophages via CCR2.
abstract: Cytokine-like 1 (CYTL1) is a novel potential cytokine that was first identified in CD34(+) cells derived from bone marrow and cord blood, and it was also found using our immunogenomics strategy. The immunobiological functions of CYTL1 remain largely unknown, and its potential receptor(s) has not been identified. A previous proposed hypothesis suggested that CYTL1 had structural similarities with CCL2 and that CCR2 was a potential receptor of CYTL1. In this study, we verify that CYTL1 possesses chemotactic activity and demonstrate that its functional receptor is CCR2B using a series of experiments performed in HEK293 cells expressing CCR2B or CCR2B-EGFP, including chemotaxis, receptor internalization, and radioactive binding assays. CYTL1 chemoattracts human monocytes but not PBLs, and its chemotactic activity toward monocytes is dependent on the CCR2B-ERK pathway. Furthermore, both human and mouse recombinant CYTL1 protein have chemotactic effects on macrophages from wild-type mice but not from Ccr2(-/-) mice. Furthermore, the chemotactic activity of CYTL1 is sensitive to pertussis toxin. All of the above data confirm that CCR2B is a functional receptor of CYTL1.
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China; and Peking University Center for Human Disease Genomics, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China; and Peking University Center for Human Disease Genomics, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China; and Peking University Center for Human Disease Genomics, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China; and Peking University Center for Human Disease Genomics, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Wanqiong
authors.initials: W
authors.lastname: Yuan
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China; and Peking University Center for Human Disease Genomics, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China; and Peking University Center for Human Disease Genomics, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China; and Peking University Center for Human Disease Genomics, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Pingzhang
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China; and.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China; and Peking University Center for Human Disease Genomics, Peking University Health Science Center, Beijing 100191, China hanwl@bjmu.edu.cn.
authors.forename: Wenling
authors.initials: W
authors.lastname: Han
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D018932:Chemokine CCL2 / Q000378:metabolism; D002633:Chemotaxis; D016207:Cytokines / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*; D020935:MAP Kinase Signaling System; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009000:Monocytes / Q000276:immunology*; D011485:Protein Binding; D054390:Receptors, CCR2 / Q000235:genetics / Q000378:metabolism*
chemical_list: D001798:Blood Proteins; C412483:CYTL1 protein, human; D018932:Chemokine CCL2; C000621450:Cytl1 protein, mouse; D016207:Cytokines; D054390:Receptors, CCR2