title: A novel immune-tolerable and permeable lectin-like protein from mushroom Agaricus bisporus.
abstract: A lectin like protein designated as LSMT is recently discovered in Agaricus bisporus. The protein adopts very similar structure to Ricin-B like lectin from Clitocybe nebularis (CNL) and HA-33 from Clostridium botulinum (HA-33), which both recognize sugar molecules that decorate the surface of the epithelial cells of the intestine. A preliminary study in silico pointed out potential capability of LSMT to perform such biological activity. Following that hypothesis, we demonstrated that LSMT is indeed capable of penetrating out from a dialysis tube of the mice intestine origin. Furthermore, the protein appeared not to evoke the immune response upon introduction into mice, unlike its structural homologs. This is the first report on the biological implication of LSMT that might lead to its application.
authors.affiliation: Dexa Laboratories of Molecular Sciences, Industri Selatan V PP-7, JABABEKA II Industrial Estate, Cikarang 17550, Indonesia. Electronic address: wangsa.ismaya@dexa-medica.com.
authors.forename: Wangsa T
authors.initials: WT
authors.lastname: Ismaya
authors.affiliation: Research Group of Pharmaceutics, School of Pharmacy, Bandung Institute of Technology, Ganesa 10, Bandung 40132, Indonesia.
authors.lastname: Yunita
authors.affiliation: Research Group of Pharmaceutics, School of Pharmacy, Bandung Institute of Technology, Ganesa 10, Bandung 40132, Indonesia.
authors.forename: Alida
authors.initials: A
authors.lastname: Efthyani
authors.affiliation: European Synchrotron Radiation Facility, 71 Avenue des Martyrs, 38000 Grenoble, France; Laboratory of Biophysical Chemistry, University of Groningen, Nijenborgh 7, 9747AG Groningen, The Netherlands.
authors.forename: Xuelei
authors.initials: X
authors.lastname: Lai
authors.affiliation: Research Group of Biotechnology, School of Pharmacy, Bandung Institute of Technology, Ganesa 10, Bandung 40132, Indonesia.
authors.forename: Debbie S
authors.initials: DS
authors.lastname: Retnoningrum
authors.affiliation: Research Group of Pharmaceutics, School of Pharmacy, Bandung Institute of Technology, Ganesa 10, Bandung 40132, Indonesia.
authors.forename: Heni
authors.initials: H
authors.lastname: Rachmawati
authors.affiliation: European Synchrotron Radiation Facility, 71 Avenue des Martyrs, 38000 Grenoble, France; Laboratory of Biophysical Chemistry, University of Groningen, Nijenborgh 7, 9747AG Groningen, The Netherlands.
authors.forename: Bauke W
authors.initials: BW
authors.lastname: Dijkstra
authors.affiliation: Dexa Laboratories of Molecular Sciences, Industri Selatan V PP-7, JABABEKA II Industrial Estate, Cikarang 17550, Indonesia.
authors.forename: Raymond R
authors.initials: RR
authors.lastname: Tjandrawinata
mesh_terms: D000818:Animals; D003198:Computer Simulation; D005260:Female; D007108:Immune Tolerance / Q000276:immunology*; D007408:Intestinal Absorption / Q000276:immunology*; D037102:Lectins / Q000737:chemistry* / Q000145:classification / Q000276:immunology*; D051379:Mice; D008956:Models, Chemical; D018448:Models, Immunological; D010539:Permeability; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid
chemical_list: C041433:Agaricus lectins; D037102:Lectins
keywords: Agaricus bisporus lectin-like protein; Mushroom tyrosinase light subunit; ORF239342