title: Aspergillus asper sp. nov. and Aspergillus collinsii sp. nov., from Aspergillus section Usti.
abstract: In sampling fungi from the built environment, two isolates that could not confidently be placed in described species were encountered. Phenotypic analysis suggested that they belonged in Aspergillus sect. Usti. In order to verify the sectional placement and to assure that they were undescribed rather than phenotypically aberrant isolates, DNA was isolated and sequenced at the beta-tubulin, calmodulin, internal transcribed spacer and RNA polymerase II loci and sequences compared with those from other species in the genus Aspergillus. At each locus, each new isolate was distant from existing species. Phylogenetic trees calculated from these data and GenBank data for species of the section Usti excluded the placement of these isolates in existing species, with statistical support. Because they were excluded from existing taxa, the distinct species Aspergillus asper (type strain NRRL 35910T) and Aspergillus collinsii (type strain NRRL 66196T) in sect. Usti are proposed to accommodate these strains.
authors.affiliation: EMSL Analytical, Inc., 200 North Route 130, Cinnaminson, NJ 08077, USA.
authors.forename: Zeljko
authors.initials: Z
authors.lastname: Jurjevic
authors.affiliation: Mycotoxin Prevention and Applied Microbiology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U. S. Department of Agriculture, 1815 North University Street, Peoria, IL 61604, USA.
authors.forename: Stephen W
authors.initials: SW
authors.lastname: Peterson
mesh_terms: D000391:Air Microbiology; D001230:Aspergillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D002140:California; D002147:Calmodulin / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D006798:Housing; D010414:Pennsylvania; D010802:Phylogeny; D012319:RNA Polymerase II / Q000235:genetics; D017422:Sequence Analysis, DNA; D014404:Tubulin / Q000235:genetics
chemical_list: D002147:Calmodulin; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D014404:Tubulin; D012319:RNA Polymerase II
keywords: Systematics; built environment; fungi; section Usti