title: Characterization of Puumala hantavirus in bank voles from two regions in the Netherlands where human cases occurred.
abstract: Puumala hantavirus (PUUV) is the most common and widespread hantavirus in Europe and is associated with a mild form of haemorrhagic fever with renal syndrome in humans, called nephropathia epidemica. This study presents the molecular characterization of PUUV circulating in bank voles in two regions of the Netherlands. Most human cases of hantavirus infection are from these two regions. Phylogenetic analysis of the (partial) S, M and L-segments indicated that the Dutch strains belong to the CE lineage, which includes PUUV strains from France, Germany and Belgium. We have identified two distinct groups of PUUV, corresponding with their geographic origin and with adjoining regions in neighbouring countries.
authors.affiliation: Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands.
authors.forename: A
authors.initials: A
authors.lastname: de Vries
authors.affiliation: Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands.
authors.forename: H
authors.initials: H
authors.lastname: Vennema
authors.affiliation: Dutch Mammal Society, P.O. Box 6531, NL-6503 GA Nijmegen, The Netherlands.
authors.forename: D L
authors.initials: DL
authors.lastname: Bekker
authors.affiliation: Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: Maas
authors.affiliation: Fluidigm, Europe B.V, Amsterdam, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Adema
authors.affiliation: Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: Opsteegh
authors.affiliation: Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands.
authors.forename: J W B
authors.initials: JWB
authors.lastname: van der Giessen
authors.affiliation: Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands.|Department of Viroscience, WHO Collaborating Centre for Arbovirus and Haemorrhagic Fever Reference and Research, Erasmus MC, Rotterdam, The Netherlands.
authors.forename: C B E M
authors.initials: CBEM
authors.lastname: Reusken
mesh_terms: D000818:Animals; D003411:Arvicolinae / Q000821:virology*; D001483:Base Sequence; D014644:Genetic Variation / Q000235:genetics; D006480:Hemorrhagic Fever with Renal Syndrome / Q000821:virology*; D006801:Humans; D009426:Netherlands; D029262:Puumala virus / Q000145:classification* / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D017423:Sequence Analysis, RNA
chemical_list: D012367:RNA, Viral