title: Analysis on the transcriptome information of two different wheat mutants and identification of salt-induced differential genes.
abstract: This study established a wheat transcriptome library using RH8706-49 and RH8706-34. Salt-induced differential genes were screened by Illumina RNA sequencing (RNA-Seq). Five differential genes were chosen to study the functions by combining transcript sequencing result and gene chip. The expression changes of these five differential genes were analyzed using real-time quantitative PCR (qRT-PCR) technique to determine the reliability and accuracy of transcriptome sequencing and transplanted into Arabidopsis thaliana to obtain transgenic homozygote plants for the salt tolerance test. The salt tolerance test results show that the transgenic plants grew far better than the wild-type plant.
authors.affiliation: College of Life Science, Hebei Normal University, Shijiazhuang 050024 Hebei, PR China. Electronic address: maxiaoli04@163.com.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Ma
authors.affiliation: College of Life Science, Hebei Normal University, Shijiazhuang 050024 Hebei, PR China. Electronic address: jiajiajia343434@163.com.
authors.forename: Peihan
authors.initials: P
authors.lastname: Gu
authors.affiliation: College of Life Science, Hebei Normal University, Shijiazhuang 050024 Hebei, PR China; College of Clinical Medicine, North China University of Science and Technology, Tangshan 063000 Hebei, PR China. Electronic address: liangwenji_2005@163.com.
authors.forename: Wenji
authors.initials: W
authors.lastname: Liang
authors.affiliation: College of Life Science, Hebei Normal University, Shijiazhuang 050024 Hebei, PR China. Electronic address: 10303613@qq.com.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Life Science, Hebei Normal University, Shijiazhuang 050024 Hebei, PR China. Electronic address: 546884699@qq.com.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Jin
authors.affiliation: College of Life Science, Hebei Normal University, Shijiazhuang 050024 Hebei, PR China. Electronic address: 116575217@qq.com.
authors.forename: Shaoxiong
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Life Science, Hebei Normal University, Shijiazhuang 050024 Hebei, PR China. Electronic address: 626869522@qq.com.
authors.forename: Yinzhu
authors.initials: Y
authors.lastname: Shen
authors.affiliation: College of Life Science, Hebei Normal University, Shijiazhuang 050024 Hebei, PR China. Electronic address: huangzhanjing@mail.hebtu.edu.cn.
authors.forename: Zhanjing
authors.initials: Z
authors.lastname: Huang
mesh_terms: D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics*; D009154:Mutation / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics; D055051:Salt-Tolerant Plants / Q000187:drug effects / Q000235:genetics*; D012965:Sodium Chloride / Q000008:administration & dosage*; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D059467:Transcriptome / Q000187:drug effects / Q000235:genetics*; D014908:Triticum / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D012965:Sodium Chloride
keywords: Salt tolerance; Transcriptome; Triticum aestivum; qRT-PCR