title: Mps1 (Monopolar Spindle 1) Protein Inhibition Affects Cellular Growth and Pro-Embryogenic Masses Morphology in Embryogenic Cultures of Araucaria angustifolia (Araucariaceae).
abstract: Somatic embryogenesis has been shown to be an efficient tool for studying processes based on cell growth and development. The fine regulation of the cell cycle is essential for proper embryo formation during the process of somatic embryogenesis. The aims of the present work were to identify and perform a structural and functional characterization of Mps1 and to analyze the effects of the inhibition of this protein on cellular growth and pro-embryogenic mass (PEM) morphology in embryogenic cultures of A. angustifolia. A single-copy Mps1 gene named AaMps1 was retrieved from the A. angustifolia transcriptome database, and through a mass spectrometry approach, AaMps1 was identified and quantified in embryogenic cultures. The Mps1 inhibitor SP600125 (10 μM) inhibited cellular growth and changed PEMs, and these effects were accompanied by a reduction in AaMps1 protein levels in embryogenic cultures. Our work has identified the Mps1 protein in a gymnosperm species for the first time, and we have shown that inhibiting Mps1 affects cellular growth and PEM differentiation during A. angustifolia somatic embryogenesis. These data will be useful for better understanding cell cycle control during somatic embryogenesis in plants.
authors.affiliation: Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brazil.
authors.forename: Jackellinne C
authors.initials: JC
authors.lastname: Douétts-Peres
authors.affiliation: Laboratório de Biotecnologia Vegetal, Núcleo em Ecologia e Desenvolvimento Sócio-ambiental de Macaé, Universidade Federal do Rio de Janeiro, Macaé, Rio de Janeiro, Brazil.
authors.forename: Marco Antônio L
authors.initials: MA
authors.lastname: Cruz
authors.affiliation: Laboratório de Biotecnologia, CBB, UENF, Campos dos Goytacazes, Rio de Janeiro, Brazil.|Unidade de Biologia Integrativa, Setor de Proteômica, UENF, Campos dos Goytacazes, Rio de Janeiro, Brazil.
authors.forename: Ricardo S
authors.initials: RS
authors.lastname: Reis
authors.affiliation: Laboratório de Biotecnologia, CBB, UENF, Campos dos Goytacazes, Rio de Janeiro, Brazil.|Unidade de Biologia Integrativa, Setor de Proteômica, UENF, Campos dos Goytacazes, Rio de Janeiro, Brazil.
authors.forename: Angelo S
authors.initials: AS
authors.lastname: Heringer
authors.affiliation: Laboratório de Biotecnologia Vegetal, Núcleo em Ecologia e Desenvolvimento Sócio-ambiental de Macaé, Universidade Federal do Rio de Janeiro, Macaé, Rio de Janeiro, Brazil.
authors.forename: Eduardo A G
authors.initials: EA
authors.lastname: de Oliveira
authors.affiliation: Laboratório de Biologia Celular de Plantas, Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo, São Paulo, Brazil.
authors.forename: Paula M
authors.initials: PM
authors.lastname: Elbl
authors.affiliation: Laboratório de Biologia Celular de Plantas, Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo, São Paulo, Brazil.
authors.forename: Eny I S
authors.initials: EI
authors.lastname: Floh
authors.affiliation: Laboratório de Biotecnologia, CBB, UENF, Campos dos Goytacazes, Rio de Janeiro, Brazil.|Unidade de Biologia Integrativa, Setor de Proteômica, UENF, Campos dos Goytacazes, Rio de Janeiro, Brazil.
authors.forename: Vanildo
authors.initials: V
authors.lastname: Silveira
authors.affiliation: Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brazil.
authors.forename: Claudete
authors.identifier: http://orcid.org/0000-0002-1669-660X
authors.initials: C
authors.lastname: Santa-Catarina
mesh_terms: D018929:Cell Culture Techniques; D049109:Cell Proliferation; D010940:Plant Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D058439:Plant Somatic Embryogenesis Techniques; D064028:Tracheophyta / Q000737:chemistry / Q000196:embryology* / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D010940:Plant Proteins