title: A vanillic acid inducible expression system for Trypanosoma brucei.
abstract: Reverse genetics in Trypanosoma brucei is dependent on the tetracycline inducible system for the precise control over the expression of both genes and dsRNA. Another independent inducible system for trypanosomes would enable the control of the activities of two different genes in the same cell, providing greater experimental sophistication. Here, I describe the development of the vanillic acid based inducible expression system for T. brucei, which operates independently of, and can be used in parallel with the tetracycline inducible system.
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, OX1 3RE, UK. Electronic address: jack.sunter@path.ox.ac.uk.
authors.forename: Jack D
authors.initials: JD
authors.lastname: Sunter
mesh_terms: D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D023061:Gene Order; D005822:Genetic Vectors / Q000235:genetics*; D009875:Operator Regions, Genetic; D011401:Promoter Regions, Genetic; D013752:Tetracycline / Q000494:pharmacology; D014346:Trypanosoma brucei brucei / Q000187:drug effects* / Q000235:genetics*; D014641:Vanillic Acid / Q000494:pharmacology*
chemical_list: D004268:DNA-Binding Proteins; D013752:Tetracycline; D014641:Vanillic Acid
keywords: Gene regulation; Inducible expression; Trypanosoma brucei; Vanillic acid