title: Cynomolgus monkeys (Macaca fascicularis) experimentally infected with B19V and hepatitis A virus: no evidence of the co-infection as a cause of acute liver failure.
abstract: This study was conducted to analyse the course and the outcome of the liver disease in the co-infected animals in order to evaluate a possible synergic effect of human parvovirus B19 (B19V) and hepatitis A virus (HAV) co-infection. Nine adult cynomolgus monkeys were inoculated with serum obtained from a fatal case of B19V infection and/or a faecal suspension of acute HAV. The presence of specific antibodies to HAV and B19V, liver enzyme levels, viraemia, haematological changes, and necroinflammatory liver lesions were used for monitoring the infections. Seroconversion was confirmed in all infected groups. A similar pattern of B19V infection to human disease was observed, which was characterised by high and persistent viraemia in association with reticulocytopenia and mild to moderate anaemia during the period of investigation (59 days). Additionally, the intranuclear inclusion bodies were observed in pro-erythroblast cell from an infected cynomolgus and B19V Ag in hepatocytes. The erythroid hypoplasia and decrease in lymphocyte counts were more evident in the co-infected group. The present results demonstrated, for the first time, the susceptibility of cynomolgus to B19V infection, but it did not show a worsening of liver histopathology in the co-infected group.
authors.affiliation: Laboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Luciane Almeida Amado
authors.initials: LA
authors.lastname: Leon
authors.affiliation: Laboratório de Controle de Neurovirulência, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Renato Sergio
authors.initials: RS
authors.lastname: Marchevsky
authors.affiliation: Laboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Ana Maria Coimbra
authors.initials: AM
authors.lastname: Gaspar
authors.affiliation: Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Universidade Federal Fluminense, Niterói, RJ, Brasil.
authors.forename: Rita de Cassia Nasser Cubel
authors.initials: Rde C
authors.lastname: Garcia
authors.affiliation: Hospital Universitário Gaffrée e Guinle, Escola de Medicina e Cirurgia, Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
authors.forename: Adilson José de
authors.initials: AJ
authors.lastname: Almeida
authors.affiliation: Laboratório de Patologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Pelajo-Machado
authors.affiliation: Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Universidade Federal Fluminense, Niterói, RJ, Brasil.
authors.forename: Tatiana Xavier de
authors.initials: TX
authors.lastname: Castro
authors.affiliation: Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Universidade Federal Fluminense, Niterói, RJ, Brasil.
authors.forename: Jussara Pereira do
authors.initials: JP
authors.lastname: Nascimento
authors.affiliation: Virus Reference Department, Health Protection Agency, London, UK.
authors.forename: Kevin E
authors.initials: KE
authors.lastname: Brown
authors.affiliation: Laboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Marcelo Alves
authors.initials: MA
authors.lastname: Pinto
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D060085:Coinfection / Q000821:virology; D004195:Disease Models, Animal; D006506:Hepatitis A / Q000150:complications* / Q000276:immunology; D030041:Hepatitis A virus / Q000276:immunology; D017114:Liver Failure, Acute / Q000821:virology*; D008252:Macaca fascicularis / Q000821:virology*; D010322:Parvoviridae Infections / Q000150:complications* / Q000276:immunology; D016732:Parvovirus B19, Human / Q000276:immunology; D014766:Viremia
chemical_list: D000914:Antibodies, Viral