title: Structural and physical properties of sanxan polysaccharide from Sphingomonas sanxanigenens.
abstract: Sphingomonas sanxanigenens, a new species of the genus Sphingomonas, synthesizes extracellular biopolymer termed sanxan. Sanxan polysaccharide was purified from the fermentation broth by Sephacryl S-400 column chromatography. The molecular weight of sanxan polysaccharide was 408kDa by the method of size-exclusion chromatography combined with laser light scattering. Based on FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments, the structure of sanxan polysaccharide was elucidated as follows: The solution of sanxan polysaccharide showed properties of high viscosity and shear-thinning. By cooling hot solutions, sanxan polysaccharide could form elastic thermoreversible gel.
authors.affiliation: College of Agronomy & Resources and Environment, Tianjin Agricultural University, Tianjin 300384, China. Electronic address: hdhuang@tjau.edu.cn.
authors.forename: Haidong
authors.initials: H
authors.lastname: Huang
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China. Electronic address: wumeng6163@163.com.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Wu
authors.affiliation: College of Agronomy & Resources and Environment, Tianjin Agricultural University, Tianjin 300384, China. Electronic address: yanghongpeng2003@163.com.
authors.forename: Hongpeng
authors.initials: H
authors.lastname: Yang
authors.affiliation: College of Agronomy & Resources and Environment, Tianjin Agricultural University, Tianjin 300384, China. Electronic address: lxy@tjau.edu.cn.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Agronomy & Resources and Environment, Tianjin Agricultural University, Tianjin 300384, China. Electronic address: 1375331479@qq.com.
authors.forename: Mengnan
authors.initials: M
authors.lastname: Ren
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China. Electronic address: gqli@nankai.edu.cn.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Li
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China. Electronic address: tingma@nankai.edu.cn.
authors.forename: Ting
authors.initials: T
authors.lastname: Ma
mesh_terms: D002240:Carbohydrate Sequence; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000737:chemistry; D055585:Physical Phenomena; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D020578:Sphingomonas / Q000737:chemistry*
chemical_list: D009005:Monosaccharides; D011135:Polysaccharides, Bacterial; D010504:Periodic Acid; C009288:metaperiodate
keywords: NMR spectroscopy; Polysaccharide; Sanxan; Sphingomonas sanxanigenens; Structure analysis