title: The dataset from administration of single or combined immunomodulation agents to modulate anti-FVIII antibody responses in FVIII plasmid or protein primed hemophilia A mice.
abstract: Hemophilia A mice with pre-existing inhibitory antibodies against factor VIII (FVIII) were treated with single agents, AMD3100 and GCS-F, respectively. Inhibitor titers in treated mice and control HemA inhibitors mice were followed over time. Total B cells and plasma cells (PCs) were characterized by flow cytometry. HemA inhibitor mice were then treated with a combination regimen of IL-2/IL-2mAb complexes plus rapamycin and AMD3100. Finally, HemA inhibitor mice were treated with a new combination therapy using include IL-2/IL-2mAb complexes + Anti-CD20+AMD3100+G-CSF. The timeline of combination therapy was illustrated. Inhibitor titers following treatment in FVIII plasmid or protein induced inhibitor mice were evaluated overtime. A representative figure and gating strategies to characterize the subsets of Treg cells and B cells are presented. Please see http://dx.doi.org/10.1016/j.cellimm.2016.01.005 [1] for interpretation and discussion of these data and results.
authors.affiliation: Center for Immunity and Immunotherapies, Seattle Children׳s Research Institute, Seattle, WA, USA; School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan.
authors.forename: Chao Lien
authors.initials: CL
authors.lastname: Liu
authors.affiliation: Center for Immunity and Immunotherapies, Seattle Children׳s Research Institute, Seattle, WA, USA.
authors.forename: Meghan J
authors.initials: MJ
authors.lastname: Lyle
authors.affiliation: Center for Immunity and Immunotherapies, Seattle Children׳s Research Institute, Seattle, WA, USA.
authors.forename: Simon C
authors.initials: SC
authors.lastname: Shin
authors.affiliation: Center for Immunity and Immunotherapies, Seattle Children׳s Research Institute, Seattle, WA, USA; Department of Pediatrics, University of Washington, Seattle, WA, USA.
authors.forename: Carol H
authors.initials: CH
authors.lastname: Miao