title: Effect of 17α-methyltestosterone (MT) on oxidation stress in the liver of juvenile GIFT tilapia, Oreochromis niloticus.
abstract: The normal dose of 17α-methyltestosterone (MT) used in fish farming was 60 mg/L, and now the analysis of residual androgens was carried out in waste water obtained from the Beijing area, which could be detected in levels ranging from 4.1 to 7.0 ng/L. For the purpose of aquatic early warning, the present study clearly demonstrated that chronic exposure by higher concentration of MT than environmental relevant concentrations could trigger oxidative stress response to juvenile tilapia by modulating hepatic antioxidant enzyme activities and gene transcription. Some antioxidative parameters (T-GSH, GSH/GSSG and MDA) were significant decreased under 0.5 mg/L MT exposure at 7 and 14 days. Some antioxidant enzymes (SOD, CAT and GST) and transcriptional changes (sod and cat) were revealed significant decreases for MT treated groups at 7 days. Total antioxidant capacity was significant increased only in 5 mg/L MT exposure groups, but GR activities were not affected all through the whole exposure period. Almost all of the antioxidant enzymatic genes detected in the present study were showed significant increments for MT exposure both at 14 and 21 days, and the genotoxicity profile of antioxidant enzymatic genes were revealed dose-dependent manner. This study presented evidence that MT could result in oxidative stress response in the early stages of GIFT tilapia.
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, No. 9 Shanshui East Rd., Wuxi, 214081 Jiangsu China ; Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Wuxi, 214081 China ; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081 China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, No. 9 Shanshui East Rd., Wuxi, 214081 Jiangsu China ; Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Wuxi, 214081 China.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Qu
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, No. 9 Shanshui East Rd., Wuxi, 214081 Jiangsu China ; Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Wuxi, 214081 China.
authors.forename: Liping
authors.initials: L
authors.lastname: Qiu
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, No. 9 Shanshui East Rd., Wuxi, 214081 Jiangsu China ; Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Wuxi, 214081 China.
authors.forename: Limin
authors.initials: L
authors.lastname: Fan
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, No. 9 Shanshui East Rd., Wuxi, 214081 Jiangsu China ; Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Wuxi, 214081 China.
authors.forename: Shunlong
authors.initials: S
authors.lastname: Meng
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, No. 9 Shanshui East Rd., Wuxi, 214081 Jiangsu China ; Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Wuxi, 214081 China.
authors.forename: Chao
authors.initials: C
authors.lastname: Song
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, No. 9 Shanshui East Rd., Wuxi, 214081 Jiangsu China ; Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Wuxi, 214081 China ; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081 China.
authors.forename: Xuwen
authors.initials: X
authors.lastname: Bing
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, No. 9 Shanshui East Rd., Wuxi, 214081 Jiangsu China ; Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Wuxi, 214081 China ; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081 China.
authors.forename: Jiazhang
authors.initials: J
authors.lastname: Chen
keywords: 17α-Methyltestosterone; Antioxidant enzyme; Genotoxicity; Oreochromis niloticus; Oxidative stress