title: Responsiveness of prepubertal crossbred bull calves to exogenous GnRH and its impact on reproductive hormones under tropical conditions.
abstract: This study investigated the age related variations in luteinizing hormone (LH), androstenedione, testosterone, and total estrogens response to exogenous gonadotropin-releasing hormone (GnRH) in Holstein-Friesian (HF) × Tharparkar bull calves. Fifteen bull calves were selected and, based on their age, were divided into Group I (14-16 months, n = 5), Group II (9-12 months, n = 5), and Group III (6-8 months, n = 5). All bull calves were administered with 10 µg of GnRH intramuscularly. Blood samples were collected at an interval of 30 min commencing 1 h prior to GnRH treatment until 4 h post-GnRH treatment and thereafter, at an interval of 1 h for the next 3 h. Endocrine response in terms of pretreatment values, peak values, area under curve, and time taken to attain peak values for LH, androstenedione, testosterone, and total estrogens was evaluated in all the bull calves. Significant differences were observed in pretreatment values, peak concentrations, and area under curve for androstenedione and testosterone between the groups; with response being higher in Group I bull calves. The results indicated that the HF × Tharparkar bull calves of 14 months age and above respond to exogenous GnRH by secreting significant amounts of testosterone.
authors.affiliation: Dairy Cattle Physiology Division, Lab No - 216, ICAR-National Dairy Research Institute, Karnal, Haryana 132001 India.
authors.forename: B S
authors.initials: BS
authors.lastname: Bharath Kumar
authors.affiliation: Dairy Cattle Physiology Division, Lab No - 216, ICAR-National Dairy Research Institute, Karnal, Haryana 132001 India.
authors.forename: Sujata
authors.initials: S
authors.lastname: Pandita
authors.affiliation: Department of Animal Physiology and Nutrition, Indian Council of Agricultural Science, Pusa, New Delhi, India.
authors.forename: B S
authors.initials: BS
authors.lastname: Prakash
authors.affiliation: Dairy Cattle Physiology Division, Lab No - 216, ICAR-National Dairy Research Institute, Karnal, Haryana 132001 India.
authors.forename: Smrutirekha
authors.initials: S
authors.lastname: Mallick
authors.affiliation: Dairy Cattle Physiology Division, Lab No - 216, ICAR-National Dairy Research Institute, Karnal, Haryana 132001 India.
authors.forename: Bhabesh
authors.initials: B
authors.lastname: Mili
keywords: Androstenedione; Gonadotropin-releasing hormone; Luteinizing hormone; Testosterone; Total estrogens