title: Helicobacter apri sp. nov., isolated from wild boars.
abstract: Three isolates (A19T, C21 and F12) with spiral-shaped cells and one bipolar sheathed flagellum were obtained from gastric mucosa and caecal contents of three different wild boars (Sus scrofa) and subjected to a polyphasic taxonomic study. A genus-specific PCR showed that these isolates belonged to the genus Helicobacter. Phylogenetic analysis based on 16S rRNA, 60-kDa heat-shock protein (HSP60) and atpA genes demonstrated they formed a novel lineage within this genus. Pairwise 16S rRNA, HSP60 and atpA gene sequence comparisons of the three isolates revealed 99.7, 99.4 and 99.9 % similarity, respectively, among the three isolates; the 16S rRNA gene of isolate A19T shared 98.5 % sequence similarity with its nearest validly named neighbouring species, Helicobacter mastomyrinus (to the type strain MIT 97-5577T). The taxonomic uniqueness of the wild boar isolates was confirmed by protein analysis performed by matrix-assisted laser desorption/ionization time-of-flight MS and by a distinctive biochemical profile. These data indicated that isolates A19T, C21 and F12 represent a novel taxon, for which the name Helicobacter apri sp. nov. is proposed, with isolate A19T (=DSM 28990T=LMG 28471T) as the type strain.
authors.affiliation: Department of Veterinary Medical Sciences, Alma Mater Studiorum, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Bologna, Italy.
authors.forename: Renato Giulio
authors.initials: RG
authors.lastname: Zanoni
authors.affiliation: Department of Veterinary Medical Sciences, Alma Mater Studiorum, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Bologna, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Piva
authors.affiliation: Department of Veterinary Medical Sciences, Alma Mater Studiorum, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Bologna, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Florio
authors.affiliation: Department of Veterinary Medical Sciences, Alma Mater Studiorum, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Bologna, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Bassi
authors.affiliation: Department of Veterinary Medical Sciences, Alma Mater Studiorum, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Bologna, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Mion
authors.affiliation: Department of Biochemistry and Microbiology, Faculty of Sciences, , Ghent University, K.L.Ledeganckstraat 35, B-9000 Gent, Belgium.
authors.forename: Margo
authors.initials: M
authors.lastname: Cnockaert
authors.affiliation: Dipartimento di Scienze Biologiche, Geologiche e Ambientali (BiGeA), Alma Mater Studiorum, Università di Bologna, Via Selmi 3, 40126 Bologna, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Luchetti
authors.affiliation: Department of Biochemistry and Microbiology, Faculty of Sciences, , Ghent University, K.L.Ledeganckstraat 35, B-9000 Gent, Belgium.
authors.forename: Peter
authors.initials: P
authors.lastname: Vandamme
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D018834:Chaperonin 60 / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005753:Gastric Mucosa / Q000382:microbiology*; D005798:Genes, Bacterial; D016998:Helicobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007558:Italy; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D034421:Sus scrofa / Q000382:microbiology*
chemical_list: D018834:Chaperonin 60; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S