title: Effect of Water Content in Extraction Mixture on the Pressurized Liquid Extraction Efficiency-Stability of Quercetin 4'-Glucoside During Extraction from Onions.
abstract: Pressurized liquid extraction (PLE) is considered as an effective and quick sample preparation method, especially in plant analysis. This quick extraction process favors the isolation of compounds for which long exposure time in high temperatures is undesirable. However, despite this advantage, the application of PLE for plant compounds extraction involves certain problems, such as instability of the compounds. The obtained data proved that even in short-lasting PLE, quercetin 4'-glucoside undergoing the hydrolytical degradation process produces the corresponding aglycon quercetin when water is a component of the applied extractant. The increase of water content in methanol-water extractant mixture heightens the transformation degree of quercetin 4'-glucoside into quercetin and increases its concentration in the PLE extracts. The degradation is accelerated by increased extraction temperature and time. In plant samples, the degradation of quercetin 4'-glucoside is much higher because of the plant matrix effects. The presented results explain why extraction of quercetin from plants by pure methanol is less efficient than by water containing alcohol solutions. More importantly, they prove that the application of water-alcohol extractant during sample preparation leads to the hydrolytical degradation of glycosides to aglycones, and, as a consequence, to false conclusions in plant analysis.