title: Evaluation of genotoxicity of general anesthesia maintained with desflurane in patients under minor surgery.
abstract: There is controversy over the genotoxic effects of volatile anesthetics. The available literature on the genotoxicity of desflurane, one of the newest volatile halogenated agents used for general anesthesia maintenance, is scarce. This study aimed to evaluate the genotoxic potential of desflurane in 15 patients without comorbidities, of both sexes, who underwent minor surgeries lasting at least 90 min. Patients enrolled in the study received desflurane anesthesia (6%); blood samples were collected before anesthesia induction (T0), 90 min after the beginning of anesthesia (T1), and on the day following surgery (T2). DNA damage was evaluated in lymphocytes using the alkaline comet assay. We found statistically significant increases in DNA damage in T2 samples compared to T0. The findings suggest that desflurane anesthesia induces DNA strand breaks/alkali-labile sites on the day after minimally invasive surgery in healthy patients.
authors.affiliation: Faculdade de Medicina de Botucatu, UNESP - Univ Estadual Paulista, Departamento de Anestesiologia, Botucatu, SP, Brazil.
authors.forename: Flávia R
authors.initials: FR
authors.lastname: Nogueira
authors.affiliation: Faculdade de Medicina de Botucatu, UNESP - Univ Estadual Paulista, Departamento de Anestesiologia, Botucatu, SP, Brazil.
authors.forename: Leandro G
authors.initials: LG
authors.lastname: Braz
authors.affiliation: Faculdade de Medicina de Botucatu, UNESP - Univ Estadual Paulista, Departamento de Anestesiologia, Botucatu, SP, Brazil.
authors.forename: Leonardo R
authors.initials: LR
authors.lastname: de Andrade
authors.affiliation: Faculdade de Medicina de Botucatu, UNESP - Univ Estadual Paulista, Departamento de Anestesiologia, Botucatu, SP, Brazil.
authors.forename: Ana Lygia R
authors.initials: AL
authors.lastname: de Carvalho
authors.affiliation: Faculdade de Medicina de Botucatu, UNESP - Univ Estadual Paulista, Departamento de Anestesiologia, Botucatu, SP, Brazil.
authors.forename: Luiz A
authors.initials: LA
authors.lastname: Vane
authors.affiliation: Faculdade de Medicina de Botucatu, UNESP - Univ Estadual Paulista, Departamento de Anestesiologia, Botucatu, SP, Brazil.
authors.forename: Norma Sueli P
authors.initials: NS
authors.lastname: Módolo
authors.affiliation: Faculdade de Medicina de Botucatu, UNESP - Univ Estadual Paulista, Departamento de Anestesiologia, Botucatu, SP, Brazil.
authors.forename: Aline G
authors.initials: AG
authors.lastname: Aun
authors.affiliation: Faculdade de Medicina de Botucatu, UNESP - Univ Estadual Paulista, Departamento de Anestesiologia, Botucatu, SP, Brazil.
authors.forename: Kátina M
authors.initials: KM
authors.lastname: Souza
authors.affiliation: Faculdade de Medicina de Botucatu, UNESP - Univ Estadual Paulista, Departamento de Anestesiologia, Botucatu, SP, Brazil.
authors.forename: José Reinaldo C
authors.initials: JR
authors.lastname: Braz
authors.affiliation: Faculdade de Medicina de Botucatu, UNESP - Univ Estadual Paulista, Departamento de Anestesiologia, Botucatu, SP, Brazil.
authors.forename: Mariana G
authors.initials: MG
authors.lastname: Braz
mesh_terms: D000293:Adolescent; D000328:Adult; D000768:Anesthesia, General; D018685:Anesthetics, Inhalation / Q000633:toxicity*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D000077335:Desflurane; D005260:Female; D006801:Humans; D007530:Isoflurane / Q000031:analogs & derivatives* / Q000633:toxicity; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D013511:Minor Surgical Procedures; D055815:Young Adult
chemical_list: D018685:Anesthetics, Inhalation; D000077335:Desflurane; D007530:Isoflurane
keywords: DNA damage; comet assay; elective surgical procedures; inhalation anesthetics