title: Assessment of immune reconstitution status in recipients of a successful hematopoietic stem cell transplant from peripheral blood after reduced intensity conditioning.
abstract: OBJECTIVE
  To document immune reconstitution status after hematopoietic stem cell transplantation (HSCT) for malignant hematologic diseases.
  
  
  METHODS
  Hematology patients who received a reduced intensity conditioning (RIC) were followed after successful allogeneic or autologous HSCT. Patients had at least 100days post-transplant. T, B and NK cells in peripheral blood (PB), and CD34+, CD133+ progenitor cells in bone marrow (BM) and peripheral blood (PB) were determined by flow cytometry.
  
  
  RESULTS
  Twenty-seven HSCT recipients, 19 allogeneic and 8 autologous, were studied at a median 155 (100-721) days post-transplant. In the whole group the median value of CD34+ cells was 1.03% in the bone marrow and 0.04% in PB, whereas values for CD133+ cells were 0.39% and 0.13%, respectively, without statistical differences between autologous and allogeneic recipients. Significantly more B cells (CD3-/CD56-/CD19+) were found in the autologous compared to the allogeneic group, 12.6 vs. 5.01, p=0.04. An increased number of CD8+ lymphocytes with a 0.63 CD4:CD8 relationship was documented in PB.
  
  
  CONCLUSIONS
  In clinically recovered autologous and allogeneic HSCT recipients BM and PB CD34+/CD133+ hematoprogenitor homeostasis is maintained within normal ranges, with better B-cell reconstitution in the autologous group.
authors.affiliation: Department of Hematology, Internal Medicine Division, "Dr. José E. González" University Hospital of the School of Medicine of the Universidad Autónoma de Nuevo León, Monterrey, México. Electronic address: carjaime@hotmail.com.
authors.forename: José C
authors.initials: JC
authors.lastname: Jaime-Pérez
authors.affiliation: Department of Hematology, Internal Medicine Division, "Dr. José E. González" University Hospital of the School of Medicine of the Universidad Autónoma de Nuevo León, Monterrey, México.
authors.forename: César D
authors.initials: CD
authors.lastname: Villarreal-Villarreal
authors.affiliation: Department of Hematology, Internal Medicine Division, "Dr. José E. González" University Hospital of the School of Medicine of the Universidad Autónoma de Nuevo León, Monterrey, México.
authors.forename: Nereida
authors.initials: N
authors.lastname: Méndez-Ramírez
authors.affiliation: Department of Hematology, Internal Medicine Division, "Dr. José E. González" University Hospital of the School of Medicine of the Universidad Autónoma de Nuevo León, Monterrey, México.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Vázquez-Garza
authors.affiliation: Department of Hematology, Internal Medicine Division, "Dr. José E. González" University Hospital of the School of Medicine of the Universidad Autónoma de Nuevo León, Monterrey, México.
authors.forename: Rosario
authors.initials: R
authors.lastname: Salazar-Riojas
authors.affiliation: Department of Hematology, Internal Medicine Division, "Dr. José E. González" University Hospital of the School of Medicine of the Universidad Autónoma de Nuevo León, Monterrey, México.
authors.forename: David
authors.initials: D
authors.lastname: Gómez-Almaguer
mesh_terms: D000071916:AC133 Antigen / Q000032:analysis; D000293:Adolescent; D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology; D016026:Bone Marrow Transplantation / Q000379:methods; D016516:CD4-CD8 Ratio; D002648:Child; D002675:Child, Preschool; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000276:immunology; D006801:Humans; D007109:Immunity; D007694:Killer Cells, Natural / Q000276:immunology; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology; D019172:Transplantation Conditioning / Q000379:methods*; D014184:Transplantation, Homologous / Q000379:methods; D055815:Young Adult
chemical_list: D000071916:AC133 Antigen; D018952:Antigens, CD34
keywords: Allogeneic transplant; Autologous transplant; Bone marrow; HSCT; Immune reconstitution