title: [Cellular Uptake and Localization of Novel NSCLC Penetrating Peptide with Neuropilin-1 Binding Motif].
abstract: OBJECTIVE
  To synthesize and study the specific binding affinity of tumor-penetrating peptide YCCS to non-small cell lung carcinoma (NSCLC) cells in vitro.
  
  
  METHODS
  YCCS peptide was designed by fusing the neuropilin-1 (NRP-1) binding sequence and NSCLC binding peptide CS. YCCS peptide was synthesized and fluorescent labeled with N-terminal FITC. NRP-1 positive human NSCLC cell A549, NRP-1 positive human breast cancer cell MDA-MB-231, normal human bronchial epithelium HBE135-E6E7 and human liver cell HL-7702 were incubated respectively, then we observed the specific binding affinity of tumor-penetrating peptide YCCS to NSCLC cells.
  
  
  RESULTS
  After treated with 5 μmol/L peptide, significant fluorescent signals of FITC-YCCS peptide were demonstrated only in NSCLC A549 cells but marginal captured signal in MDA-MB-231, normal human HBE135-E6E7 or HL-7702 cells, which revealed specific NSCLC cells binding affinity. In 20 μmol/L treated group, non-specific binding were found in MDA-MB-231 and HL-7702 cells.
  
  
  CONCLUSIONS
  The results of this novel designed YCCS peptide indicated a promising strategy for improving tumor penetrating with delivery capability of drugs to NSCLC A549 cells when treated with 5 μmol/L peptide.
authors.forename: Hui-jun
authors.initials: HJ
authors.lastname: Zhou
authors.forename: Ping
authors.initials: P
authors.lastname: Dong
authors.forename: Hua-wei
authors.initials: HW
authors.lastname: Cai
authors.forename: Xiao-ai
authors.initials: XA
authors.lastname: Wu
authors.forename: Wen-jie
authors.initials: WJ
authors.lastname: Zhang
authors.forename: Fu-wen
authors.initials: FW
authors.lastname: Pang
authors.forename: Lin
authors.initials: L
authors.lastname: Li
mesh_terms: D001665:Binding Sites; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism*; D045744:Cell Line, Tumor; D057846:Cell-Penetrating Peptides / Q000378:metabolism*; D004337:Drug Carriers / Q000378:metabolism; D006801:Humans; D039942:Neuropilin-1 / Q000737:chemistry*
chemical_list: D057846:Cell-Penetrating Peptides; D004337:Drug Carriers; D039942:Neuropilin-1