title: Radix Ilicis Pubescentis total flavonoids combined with mobilization of bone marrow stem cells to protect against cerebral ischemia/reperfusion injury.
abstract: Previous studies have shown that Radix Ilicis Pubescentis total flavonoids have a neuroprotective effect, but it remains unclear whether Radix Ilicis Pubescentis total flavonoids have a synergistic effect with the recombinant human granulocyte colony stimulating factor-mobilized bone marrow stem cell transplantation on cerebral ischemia/reperfusion injury. Rat ischemia models were administered 0.3, 0.15 and 0.075 g/kg Radix Ilicis Pubescentis total flavonoids from 3 days before modeling to 2 days after injury. Results showed that Radix Ilicis Pubescentis total flavonoids could reduce pathological injury in rats with cerebral ischemia/reperfusion injury. The number of Nissl bodies increased, Bax protein expression decreased, Bcl-2 protein expression increased and the number of CD34-positive cells increased. Therefore, Radix Ilicis Pubescentis total flavonoids can improve the bone marrow stem cell mobilization effect, enhance the anti-apoptotic ability of nerve cells, and have a neuroprotective effect on cerebral ischemia/reperfusion injury in rats.
authors.affiliation: Henan University of Traditional Chinese Medicine, Zhengzhou, Henan Province, China.
authors.forename: Ming-San
authors.identifier: 0000-0003-2681-4291
authors.initials: MS
authors.lastname: Miao
authors.affiliation: Henan University of Traditional Chinese Medicine, Zhengzhou, Henan Province, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Guo
authors.affiliation: Henan University of Traditional Chinese Medicine, Zhengzhou, Henan Province, China.
authors.forename: Rui-Qi
authors.initials: RQ
authors.lastname: Li
authors.affiliation: Henan University of Traditional Chinese Medicine, Zhengzhou, Henan Province, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Ma
keywords: Bax; Bcl-2; CD34; Nissl bodies; Radix Ilicis Pubescentis total flavonoids; bone marrow stem cells; cerebral ischemia/reperfusion; mobilization; nerve regeneration; neural regeneration; neurons