title: Harpagoside suppresses IL-6 expression in primary human osteoarthritis chondrocytes.
abstract: There is growing evidence in support of the involvement of inflammatory response in the pathogenesis of osteoarthritis (OA). Harpagoside, one of the bioactive components of Harpagophytum procumbens (Hp), has been shown to possess anti-inflammatory properties. Here we used an in vitro model of inflammation in OA to investigate the potential of harpagoside to suppress the production of inflammatory cytokines/chemokines such as IL-6 and matrix degrading proteases. We further investigated the likely targets of harpagoside in primary human OA chondrocytes. OA chondrocytes were pre-treated with harpagoside before stimulation with IL-1β. mRNA expression profile of 92 cytokines/chemokines was determined using TaqMan Human Chemokine PCR Array. Expression levels of selected mRNAs were confirmed using TaqMan assays. Protein levels of IL-6 and MMP-13 were assayed by ELISA and immunoblotting. Total protein levels and phosphorylation of signaling proteins were determined by immunoblotting. Cellular localization of IL-6 and c-Fos was performed by immunofluorescence and confocal microscopy. DNA binding activity of c-FOS/AP-1 was determined by ELISA. Harpagoside significantly altered the global chemokine expression profile in IL-1β-stimulated OA chondrocytes. Expression of IL-6 was highly induced by IL-1β, which was significantly inhibited by pre-treatment of OA chondrocytes with harpagoside. Harpagoside did not inhibit the IL-1β-induced activation of NF-κB and C/EBPβ transcription factors but suppressed the IL-1β-triggered induction, phosphorylation, and DNA binding activity of c-FOS, one of the main components of AP-1 transcription factors. Further, harpagoside significantly inhibited the expression of MMP-13 in OA chondrocytes under pathological conditions. siRNA-mediated knockdown of IL-6 resulted in suppressed expression and secretion of MMP-13 directly linking the role of IL-6 with MMP-13 expression. Taken together, the present study suggests that harpagoside exerts a significant anti-inflammatory effect by inhibiting the inflammatory stimuli mediated by suppressing c-FOS/AP-1 activity in OA chondrocytes under pathological conditions. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:311-320, 2017.
authors.affiliation: Department of Anatomy and Neurobiology, North East Ohio Medical University, 4209 State Route 44, Rootstown, Ohio, 44272.
authors.forename: Abdul
authors.initials: A
authors.lastname: Haseeb
authors.affiliation: Department of Anatomy and Neurobiology, North East Ohio Medical University, 4209 State Route 44, Rootstown, Ohio, 44272.
authors.forename: Mohammad Yunus
authors.initials: MY
authors.lastname: Ansari
authors.affiliation: Department of Anatomy and Neurobiology, North East Ohio Medical University, 4209 State Route 44, Rootstown, Ohio, 44272.
authors.forename: Tariq M
authors.initials: TM
authors.lastname: Haqqi
mesh_terms: D022782:CCAAT-Enhancer-Binding Protein-beta / Q000378:metabolism; D018925:Chemokines / Q000378:metabolism; D019902:Chondrocytes / Q000187:drug effects* / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D006027:Glycosides / Q000494:pharmacology / Q000627:therapeutic use*; D031684:Harpagophytum; D006801:Humans; D053583:Interleukin-1beta; D015850:Interleukin-6 / Q000378:metabolism*; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D010003:Osteoarthritis / Q000188:drug therapy* / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D061251:Primary Cell Culture; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D011714:Pyrans / Q000494:pharmacology / Q000627:therapeutic use*; D017382:Reactive Oxygen Species / Q000378:metabolism; D018808:Transcription Factor AP-1 / Q000378:metabolism; D000093242:Interleukin-6 Inhibitors
chemical_list: D022782:CCAAT-Enhancer-Binding Protein-beta; D018925:Chemokines; D006027:Glycosides; D053583:Interleukin-1beta; D015850:Interleukin-6; D053509:Matrix Metalloproteinase 13; D016328:NF-kappa B; D010936:Plant Extracts; D016760:Proto-Oncogene Proteins c-fos; D011714:Pyrans; D017382:Reactive Oxygen Species; D018808:Transcription Factor AP-1; D000093242:Interleukin-6 Inhibitors; C508600:IL6 protein, human; C033249:harpagoside; C507079:MMP13 protein, human
keywords: IL-6; MMP-13; c-FOS/AP-1; harpagoside; osteoarthritis