title: Pramipexole pretreatment attenuates myocardial ischemia/reperfusion injury through upregulation of autophagy.
abstract: This article investigated the effects of pramipexole on myocardial ischemia reperfusion (I/R) injury and its underlying mechanisms. We utilized an in vivo mouse model of myocardial I/R injury and an in vitro H9c2 cell model of hypoxia/reoxygenation (H/R) injury. Pramipexole pretreatment in male C57BL/6 mice significantly reduced the myocardial infarction size, decreased the CK and LDH activities at the serum level and enhanced autophagy. In the in vitro study, the pramipexole treatment significantly elevated the survival rate, decreased the LDH activity, reduced ROS generation and restored the ΔΨm in H9C2 cells during H/R. Additionally, its use could increase the autophagy flux level in H9c2 cells. The underlying mechanisms were determined by measuring the expression of the autophagic protein levels. These results further indicated that pramipexole treatment modulated H/R-induced autophagy via an AMPK-dependent pathway. All of these data indicate that pramipexole exerted protective effects against myocardial I/R injury and enhanced autophagy in part through the AMPK-mediated pathway.
authors.affiliation: Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China; Department of Internal Medicine, Yiyang Medical College, Yingbin Road 516, Yiyang, Hunan 413000, China. Electronic address: yinglimo@hotmail.com.
authors.forename: Yingli
authors.initials: Y
authors.lastname: Mo
authors.affiliation: Department of Cardiology, Zhujiang Hospital of Southern Medical University, No. 253, Gongye Road, Guangzhou, 510280, China; Department of Cardiology, Yiyang Central Hospital, Kangfu Road 118, Yiyang, Hunan 413000, China. Electronic address: broycn@live.com.
authors.forename: Lu
authors.initials: L
authors.lastname: Tang
authors.affiliation: Department of Cardiology, Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563003, China. Electronic address: mayimayizmc@126.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China. Electronic address: saizhuwuphd@hotmail.com.
authors.forename: Saizhu
authors.initials: S
authors.lastname: Wu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001343:Autophagy / Q000187:drug effects*; D052160:Benzothiazoles / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D000077487:Pramipexole; D011292:Premedication / Q000379:methods*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015427:Reperfusion Injury / Q000473:pathology / Q000503:physiopathology* / Q000517:prevention & control*; D016896:Treatment Outcome; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D017382:Reactive Oxygen Species; D000077487:Pramipexole
keywords: Autophagy; Myocardial ischemia/reperfusion injury; Pramipexole