title: Process intensification of delignification and enzymatic hydrolysis of delignified cellulosic biomass using various process intensification techniques including cavitation.
abstract: Different methods of pretreatment including alkali treatment, treatment with ultrasound, biological treatment using laccase enzyme and combined treatment like ultrasound-laccase for Napier grass have been tried. With alkali pretreatment optimized conditions obtained were sodium hydroxide 0.3% w/v giving 86% delignification at temperature of 80°C, treatment time of 2h. In physical methods of treatment ultrasound, at a temperature of 45°C, treatment time of 2h, operating at frequency 24kHz and power of 100W gave 18% delignification. For laccase pretreatment, optimized conditions obtained were 300rpm impeller speed, enzyme concentration 10U/gm of Napier grass gave 50% delignification with cellulose. The optimized conditions for delignification by using combination treatment of ultrasound & enzymatic were obtained at 24kHz frequency, 100W giving 75% of delignification in 6h. An enhancement in lignin degradation by 25% and reduction in the treatment time from 12 to 6h is achieved as compared to only laccase treatment.
authors.affiliation: Department of Chemical Engineering, Institute of Chemical Technology, Matunga, Mumbai 400 019, India.
authors.forename: Karuna Narsappa
authors.initials: KN
authors.lastname: Nagula
authors.affiliation: Department of Chemical Engineering, Institute of Chemical Technology, Matunga, Mumbai 400 019, India. Electronic address: ab.pandit@ictmumbai.edu.in.
authors.forename: Aniruddha Bhalchandra
authors.initials: AB
authors.lastname: Pandit
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002482:Cellulose / Q000737:chemistry*; D006868:Hydrolysis; D042845:Laccase / Q000737:chemistry / Q000378:metabolism; D008031:Lignin / Q000737:chemistry*; D031782:Pennisetum / Q000737:chemistry* / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry; D012972:Sodium Hydroxide / Q000737:chemistry; D013696:Temperature; D055454:Trametes / Q000201:enzymology; D014465:Ultrasonics / Q000379:methods
chemical_list: D011134:Polysaccharides; D012972:Sodium Hydroxide; C007916:hemicellulose; D002482:Cellulose; D008031:Lignin; D042845:Laccase
keywords: Cavitation; Delignification; Laccases; Napier grass