title: Heat shock transcription factor 1-associated expression of slow myosin heavy chain in mouse soleus muscle in response to unloading with or without reloading.
abstract: The effects of heat shock transcription factor 1 (HSF1) deficiency on the fibre type composition and the expression level of nuclear factor of activated T cells (NFAT) family members (NFATc1, NFATc2, NFATc3 and NFATc4), phosphorylated glycogen synthase kinase 3α (p-GSK3α) and p-GSK3β, microRNA-208b (miR-208b), miR-499 and slow myosin heavy chain (MyHC) mRNAs (Myh7 and Myh7b) of antigravitational soleus muscle in response to unloading with or without reloading were investigated.
  
  
  
  HSF1-null and wild-type mice were subjected to continuous 2-week hindlimb suspension followed by 2- or 4-week ambulation recovery.
  
  
  
  In wild-type mice, the relative population of slow type I fibres, the expression level of NFATc2, p-GSK3 (α and β), miR-208b, miR-499 and slow MyHC mRNAs (Myh7 and Myh7b) were all decreased with hindlimb suspension, but recovered after it. Significant interactions between train and time (the relative population of slow type I fibres; P = 0.01, the expression level of NFATc2; P = 0.001, p-GSKβ; P = 0.009, miR-208b; P = 0.002, miR-499; P = 0.04) suggested that these responses were suppressed in HSF1-null mice.
  
  
  
  HSF1 may be a molecule in the regulation of the expression of slow MyHC as well as miR-208b, miR-499, NFATc2 and p-GSK3 (α and β) in mouse soleus muscle.
authors.affiliation: Department of Locomotive Rehabilitation Science, Unit of Rehabilitation Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.|Laboratory of Physiology, School of Health Science, Toyohashi SOZO University, Toyohashi, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Yokoyama
authors.affiliation: Laboratory of Physiology, School of Health Science, Toyohashi SOZO University, Toyohashi, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ohno
authors.affiliation: Department of Physiology, Graduate School of Health Science, Toyohashi SOZO University, Toyohashi, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Egawa
authors.affiliation: Department of Orthopaedic Surgery, St. Marianna University School of Medicine, Kawasaki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yasuhara
authors.affiliation: Department of Molecular Biology, Graduate School of Medicine, Yamaguchi University, Ube, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Nakai
authors.affiliation: Faculty of Education, Yamaguchi University, Yamaguchi, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sugiura
authors.affiliation: Faculty and Graduate School of Health and Sports Sciences, Doshisha University, Kyotanabe, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ohira
authors.affiliation: Hirosaki Gakuin University, Hirosaki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yoshioka
authors.affiliation: Department of Locomotive Rehabilitation Science, Unit of Rehabilitation Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Okita
authors.affiliation: Department of Locomotive Rehabilitation Science, Unit of Rehabilitation Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Origuchi
authors.affiliation: Laboratory of Physiology, School of Health Science, Toyohashi SOZO University, Toyohashi, Japan.|Department of Physiology, Graduate School of Health Science, Toyohashi SOZO University, Toyohashi, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Goto
mesh_terms: D000818:Animals; D001835:Body Weight / Q000502:physiology; D038362:Glycogen Synthase Kinase 3 / Q000096:biosynthesis / Q000235:genetics; D006112:Gravitation; D000076249:Heat Shock Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D019417:Hindlimb Suspension; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics; D018657:Muscle Fibers, Slow-Twitch / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism* / Q000502:physiology*; D018995:Myosin Heavy Chains / Q000096:biosynthesis*; D050778:NFATC Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D009929:Organ Size / Q000502:physiology; D020127:Recovery of Function
chemical_list: D000076249:Heat Shock Transcription Factors; C490017:Hsf1 protein, mouse; D035683:MicroRNAs; D050778:NFATC Transcription Factors; C494007:Nfatc2 protein, mouse; D038362:Glycogen Synthase Kinase 3; C448021:glycogen synthase kinase 3 alpha; D018995:Myosin Heavy Chains
keywords: glycogen synthase kinase 3; heat shock transcription factor 1; microRNA; myosin heavy chain; nuclear factor of activated T cells