title: Fluid motion and shear forces in platelet storage bags with different modes of agitation.
abstract: This study describes a new method for comparison of fluid motion and associated shear forces with various modes of platelet agitation. Fluid motion generated by flatbed, tumbler-type and circular platelet agitators was investigated. Trajectory tracings of polyethylene beads suspended inside platelet storage bags were used to calculate motion parameters. Mean particle speeds generated by flatbed agitation were approximately 25-fold greater than those generated by 3-rpm tumbler-type agitation, corresponding to a wall shear stress increase in approximately sevenfold for flatbed agitation. As such, flatbed agitation is more likely to contribute to shear-associated changes in platelets than other modes of agitation.
authors.affiliation: Pathology and Laboratory Medicine Service, VA Connecticut Healthcare System, West Haven, CT, USA.|Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Torres
authors.affiliation: Pathology and Laboratory Medicine Service, VA Connecticut Healthcare System, West Haven, CT, USA.|Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT, USA.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Tormey
authors.affiliation: Pathology and Laboratory Medicine Service, VA Connecticut Healthcare System, West Haven, CT, USA.|Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT, USA.
authors.forename: Gary
authors.initials: G
authors.lastname: Stack
mesh_terms: D001792:Blood Platelets / Q000166:cytology* / Q000502:physiology; D001793:Blood Preservation / Q000379:methods*; D015925:Cryopreservation; D006801:Humans; D011095:Polyethylenes / Q000737:chemistry; D033081:Shear Strength
chemical_list: D011095:Polyethylenes
keywords: platelet storage; platelets; storage lesion