title: A new method to determine optimum temperature and activation energies for enzymatic reactions.
abstract: A new method for determination of the optimum temperature and activation energies based on an idea of the average rate of enzymatic reaction has been developed. A mathematical model describing the effect of temperature on a dimensionless activity for enzyme deactivation following the first-order kinetics has been derived. The necessary condition for existence of the function extreme of the optimal temperature has been applied in the model. The developed method has been verified using the experimental data for inulinase from Kluyveromyces marxianus.
authors.affiliation: Department of Chemical and Biochemical Engineering, Faculty of Chemical Technology and Engineering, University of Science and Technology, Seminaryjna 3, 85-326, Bydgoszcz, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Wojcik
authors.affiliation: Department of Chemical and Biochemical Engineering, Faculty of Chemical Technology and Engineering, University of Science and Technology, Seminaryjna 3, 85-326, Bydgoszcz, Poland. jmilek@utp.edu.pl.
authors.forename: J
authors.initials: J
authors.lastname: Miłek
mesh_terms: D004798:Enzymes / Q000378:metabolism*; D007700:Kinetics; D008962:Models, Theoretical; D013696:Temperature
chemical_list: D004798:Enzymes
keywords: Biokinetics; Enzyme activity; Enzyme deactivation; Inulinase; Kinetic parameters; Optimum temperature