title: Revealing Higher Order Protein Structure Using Mass Spectrometry.
abstract: The development of rapid, sensitive, and accurate mass spectrometric methods for measuring peptides, proteins, and even intact protein assemblies has made mass spectrometry (MS) an extraordinarily enabling tool for structural biology. Here, we provide a personal perspective of the increasingly useful role that mass spectrometric techniques are exerting during the elucidation of higher order protein structures. Areas covered in this brief perspective include MS as an enabling tool for the high resolution structural biologist, for compositional analysis of endogenous protein complexes, for stoichiometry determination, as well as for integrated approaches for the structural elucidation of protein complexes. We conclude with a vision for the future role of MS-based techniques in the development of a multi-scale molecular microscope. Graphical Abstract ᅟ.
authors.affiliation: Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, NY, 10065, USA. chait@rockefeller.edu.
authors.forename: Brian T
authors.identifier: 0000-0003-3524-557X
authors.initials: BT
authors.lastname: Chait
authors.affiliation: CBM, CNRS UPR4301, Rue Charles Sadron, CS 80054, 45071, Orleans Cedex 2, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Cadene
authors.affiliation: Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, NY, 10065, USA.
authors.forename: Paul Dominic
authors.initials: PD
authors.lastname: Olinares
authors.affiliation: Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, NY, 10065, USA.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Rout
authors.affiliation: Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, NY, 10065, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Shi
mesh_terms: D013058:Mass Spectrometry; D010455:Peptides; D011506:Proteins / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D010455:Peptides; D011506:Proteins
keywords: Electrospray ionization; Endogenous protein complexes; Folding state in solution; Higher order structure; MALDI; Mass spectrometry; Molecular microscope; Native mass spectrometry; Protein assembly; Protein complex; Protein composition; Stoichiometry; Structural biology; X-ray crystallography