title: Construction of specific magnetic resonance imaging/optical dual-modality molecular probe used for imaging angiogenesis of gastric cancer.
abstract: The purpose of the study was to construct specific magnetic resonance imaging (MRI)/optical dual-modality molecular probe. Tumor-bearing animal models were established. MRI/optical dual-modality molecular probe was construed by coupling polyethylene glycol (PEG)-modified nano-Fe3O4with specific targeted cyclopeptide GX1 and near-infrared fluorescent dyes Cy5.5. MRI/optical imaging effects of the probe were observed and the feasibility of in vivo double-modality imaging was discussed. It was found that, the double-modality probe was of high stability; tumor signal of the experimental group tended to be weak after injection of the probe, but rose to a level which was close to the previous level after 18 h (p > 0.05). We successively completed the construction of an ideal MRI/optical dual-modality molecular probe. MRI/optical dual-modality molecular probe which can selectively gather in gastric cancer is expected to be a novel probe used for diagnosing gastric cancer in the early stage.
authors.affiliation: a Department of Spleen and Stomach Diseases , Hospital of Traditional Chinese Medicine , Yantai , Shandong , China.
authors.forename: Xuejie
authors.initials: X
authors.lastname: Yan
authors.affiliation: b Emergency Department , Hospital of Traditional Chinese Medicine , Yantai , Shandong , China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Song
authors.affiliation: a Department of Spleen and Stomach Diseases , Hospital of Traditional Chinese Medicine , Yantai , Shandong , China.
authors.forename: Zhenbo
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000230:Adenocarcinoma / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D002232:Carbocyanines / Q000737:chemistry / Q000378:metabolism; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D052203:Ferrosoferric Oxide / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007279:Injections, Subcutaneous; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry; D008297:Male; D051379:Mice; D008819:Mice, Nude; D015335:Molecular Probes / Q000737:chemistry / Q000378:metabolism*; D009389:Neovascularization, Pathologic / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology; D061848:Optical Imaging / Q000379:methods*; D010456:Peptides, Cyclic / Q000737:chemistry / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry; D013274:Stomach Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology
chemical_list: C098793:CY5.5 cyanine dye; D002232:Carbocyanines; D005456:Fluorescent Dyes; D058185:Magnetite Nanoparticles; D015335:Molecular Probes; D010456:Peptides, Cyclic; D011092:Polyethylene Glycols; D052203:Ferrosoferric Oxide
keywords: Dual-modality molecular probe; GX1; gastric cancer; magnetic resonance imaging; optical imaging