title: Retroreflective Janus Microparticle as a Nonspectroscopic Optical Immunosensing Probe.
abstract: We developed retroreflective Janus microparticles (RJPs) as a novel optical immunosensing probe for use in a nonspectroscopic retroreflection-based immunoassay. By coating the metals on the hemispherical surface of silica particles, highly reflective RJPs were fabricated. On the basis of the retroreflection principle, the RJPs responded to polychromatic white light sources, in contrast to conventional optical probes, which require specific monochromatic light. The retroreflection signals from RJPs were distinctively recognized as shining dots, which can be intuitively counted using a digital camera setup. Using the developed retroreflective immunosensing system, cardiac troponin I, a specific biomarker of acute myocardial infarction, was detected with high sensitivity. On the basis of the demonstrated features of the retroreflective immunosensing platform, we expect that our approach may be applied for various point-of-care-testing applications.
authors.affiliation: Department of Applied Chemistry & Biological Engineering and ‡Department of Molecular Science & Technology, Ajou University , Suwon 443749, South Korea.
authors.forename: Yong Duk
authors.initials: YD
authors.lastname: Han
authors.affiliation: Department of Applied Chemistry & Biological Engineering and ‡Department of Molecular Science & Technology, Ajou University , Suwon 443749, South Korea.
authors.forename: Hyo-Sop
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Applied Chemistry & Biological Engineering and ‡Department of Molecular Science & Technology, Ajou University , Suwon 443749, South Korea.
authors.forename: Yoo Min
authors.initials: YM
authors.lastname: Park
authors.affiliation: Department of Applied Chemistry & Biological Engineering and ‡Department of Molecular Science & Technology, Ajou University , Suwon 443749, South Korea.
authors.forename: Hyeong Jin
authors.initials: HJ
authors.lastname: Chun
authors.affiliation: Department of Applied Chemistry & Biological Engineering and ‡Department of Molecular Science & Technology, Ajou University , Suwon 443749, South Korea.
authors.forename: Jae-Ho
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Applied Chemistry & Biological Engineering and ‡Department of Molecular Science & Technology, Ajou University , Suwon 443749, South Korea.
authors.forename: Hyun C
authors.initials: HC
authors.lastname: Yoon
mesh_terms: D015415:Biomarkers; D006801:Humans; D007118:Immunoassay; D009203:Myocardial Infarction; D049329:Nanostructures; D012822:Silicon Dioxide; D019210:Troponin I
chemical_list: D015415:Biomarkers; D019210:Troponin I; D012822:Silicon Dioxide
keywords: Janus particle; biosensors; cardiac troponin I; immunoassays; retroreflection