title: Chemical characteristics of a heteropolysaccharide from Tylopilus ballouii mushroom and its antioxidant and anti-inflammatory activities.
abstract: Fucogalactomannan (FGM) is a non-sulphated polysaccharide isolated from the Tylopilus ballouii mushroom. We investigated the chemical characteristics of this FGM using HPLC, chemical methods, and NMR studies ((1)H, (13)C, (1)H/(13)C-HSQC and DEPT-135 spectroscopies) without chemical fragmentation. This polysaccharide consisted primarily of mannose and galactose with variable amounts of fucose and traces of xylose and with MW of 140kDa. Infrared and NMR spectroscopies showed the possible interaction between these polysaccharides and proteins. The antioxidant activity showed for FGM a high inhibition of superoxide and hydroxyl radicals with an IC50 of 1.25 and 1.6mg/mL, respectively. The results of peroxidation tests showed that FGM had an IC50 of 1.72mg/mL. Furthermore, the anti-inflammatory assay showed that FGM reduced edema by 32.8%, 42.0%, and 56% at doses of 30, 50, and 70mg/kg, respectively. Thus, these results suggested a structure and indicated possible anti-inflammatory and antioxidant activities use of these polysaccharides.
authors.affiliation: Laboratory of Glycobiology, Department of Biochemistry, CB, Federal University of Rio Grande do Norte, Av. Salgado Filho 3000, Bairro L. Nova, CEP 59072-970 Natal, RN, Brazil.
authors.forename: Adriane T M
authors.initials: AT
authors.lastname: Lima
authors.affiliation: Laboratory of Glycobiology, Department of Biochemistry, CB, Federal University of Rio Grande do Norte, Av. Salgado Filho 3000, Bairro L. Nova, CEP 59072-970 Natal, RN, Brazil.
authors.forename: Marilia N
authors.initials: MN
authors.lastname: Santos
authors.affiliation: Laboratory of Glycobiology, Department of Biochemistry, CB, Federal University of Rio Grande do Norte, Av. Salgado Filho 3000, Bairro L. Nova, CEP 59072-970 Natal, RN, Brazil.
authors.forename: Leonardo A R
authors.initials: LA
authors.lastname: de Souza
authors.affiliation: Laboratory of Glycobiology, Department of Biochemistry, CB, Federal University of Rio Grande do Norte, Av. Salgado Filho 3000, Bairro L. Nova, CEP 59072-970 Natal, RN, Brazil.
authors.forename: Thuane S
authors.initials: TS
authors.lastname: Pinheiro
authors.affiliation: Laboratory of Glycobiology, Department of Biochemistry, CB, Federal University of Rio Grande do Norte, Av. Salgado Filho 3000, Bairro L. Nova, CEP 59072-970 Natal, RN, Brazil.
authors.forename: Almino A O
authors.initials: AA
authors.lastname: Paiva
authors.affiliation: Laboratory of Glycobiology, Department of Biochemistry, CB, Federal University of Rio Grande do Norte, Av. Salgado Filho 3000, Bairro L. Nova, CEP 59072-970 Natal, RN, Brazil.
authors.forename: Celina M P G
authors.initials: CM
authors.lastname: Dore
authors.affiliation: Laboratory of Glycobiology, Department of Biochemistry, CB, Federal University of Rio Grande do Norte, Av. Salgado Filho 3000, Bairro L. Nova, CEP 59072-970 Natal, RN, Brazil.
authors.forename: Mariana S S P
authors.initials: MS
authors.lastname: Costa
authors.affiliation: Laboratory of Glycobiology, Department of Biochemistry, CB, Federal University of Rio Grande do Norte, Av. Salgado Filho 3000, Bairro L. Nova, CEP 59072-970 Natal, RN, Brazil.
authors.forename: Nednaldo D
authors.initials: ND
authors.lastname: Santos
authors.affiliation: Laboratory of Mycology, Department of Botany, Zoology and Ecology, Federal University of Rio Grande do Norte, Av. Salgado Filho 3000, Bairro L. Nova, CEP 59072-970 Natal, RN, Brazil.
authors.forename: Yuri G
authors.initials: YG
authors.lastname: Baseia
authors.affiliation: Institute of Chemistry, Federal University of Rio Grande do Norte, Av. Salgado Filho 3000, Bairro L. Nova, CEP 59072-970 Natal, RN, Brazil.
authors.forename: Renata M
authors.initials: RM
authors.lastname: Araújo
authors.affiliation: Laboratory of Glycobiology, Department of Biochemistry, CB, Federal University of Rio Grande do Norte, Av. Salgado Filho 3000, Bairro L. Nova, CEP 59072-970 Natal, RN, Brazil. Electronic address: eddaleite@cb.ufrn.br.
authors.forename: Edda L
authors.initials: EL
authors.lastname: Leite
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D001487:Basidiomycota / Q000737:chemistry*; D004487:Edema / Q000188:drug therapy / Q000473:pathology; D051379:Mice; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D009005:Monosaccharides; D011134:Polysaccharides
keywords: Antioxidant activities; Fucogalactomannan; Inflammation; Mushroom; NMR; Polysaccharides; Tylopilus ballouii