title: Odorant Screening and Quantitation of Thiols in Carmenere Red Wine by Gas Chromatography-Olfactometry and Stable Isotope Dilution Assays.
abstract: The sensory impact of thiols in Vitis vinifera 'Carmenere' red wines was evaluated. For this purpose, aroma extract dilution analysis was applied to the thiols isolated from a Carmenere red wine by affinity chromatography with a mercurated agarose gel. Results revealed the presence of four odorants, identified as 2-furanylmethanethiol, 3-sulfanylhexyl acetate, 3-sulfanyl-1-hexanol, and 2-methyl-3-sulfanyl-1-butanol, with the latter being described here for the first time in Carmenere red wines. Quantitation of the four thiols in the Carmenere wine screened by aroma extract dilution analysis and in three additional Carmenere wines by stable isotope dilution assays resulted in concentrations above the respective orthonasal odor detection threshold values. Triangle tests applied to wine model solutions with and without the addition of the four thiols showed significant differences, thus suggesting that the compounds do have the potential to influence the overall aroma of red wine.
authors.affiliation: Department of Chemical and Bioprocess Engineering, School of Engineering, Pontificia Universidad Católica de Chile , Avenida Vicuña Mackenna, 4860 Macul, Santiago, Chile.|Centro de Aromas y Sabores, DICTUC , Avenida Vicuña Mackenna, 4860 Macul, Santiago, Chile.
authors.forename: Carolina
authors.initials: C
authors.lastname: Pavez
authors.affiliation: Department of Chemical and Bioprocess Engineering, School of Engineering, Pontificia Universidad Católica de Chile , Avenida Vicuña Mackenna, 4860 Macul, Santiago, Chile.|Centro de Aromas y Sabores, DICTUC , Avenida Vicuña Mackenna, 4860 Macul, Santiago, Chile.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Agosin
authors.affiliation: Deutsche Forschungsanstalt für Lebensmittelchemie (German Research Center for Food Chemistry) , Lise-Meitner-Straße 34, 85354 Freising, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Steinhaus
mesh_terms: D002849:Chromatography, Gas / Q000379:methods*; D007554:Isotopes; D009812:Odorants; D064367:Olfactometry; D013438:Sulfhydryl Compounds / Q000032:analysis*; D014920:Wine / Q000032:analysis*
chemical_list: D007554:Isotopes; D013438:Sulfhydryl Compounds
keywords: AEDA; Carmenere red wine; GC−olfactometry; SIDA; odor-active thiols; thiol quantitation