title: Influence of different buffers (HEPES/MOPS) on keratinocyte cell viability and microbial growth.
abstract: This study assessed the effect of the buffers 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 3-(N-morpholino) propanesulfonic acid (MOPS) on keratinocyte cell viability and microbial growth. It was observed that RPMI buffered with HEPES, supplemented with l-glutamine and sodium bicarbonate, can be used as a more suitable medium to promote co-culture.
authors.affiliation: Department of Dental Materials and Prosthodontics, Oral Rehabilitation Program-Araraquara School of Dentistry, UNESP-Univ. Estadual Paulista, Centro, 14801903 Araraquara, SP, Brazil. Electronic address: kassiaodonto@hotmail.com.
authors.forename: Kássia de Carvalho
authors.initials: Kde C
authors.lastname: Dias
authors.affiliation: Department of Dental Materials and Prosthodontics, Oral Rehabilitation Program-Araraquara School of Dentistry, UNESP-Univ. Estadual Paulista, Centro, 14801903 Araraquara, SP, Brazil. Electronic address: pabfarma@yahoo.com.br.
authors.forename: Paula Aboud
authors.initials: PA
authors.lastname: Barbugli
authors.affiliation: Department of Dental Materials and Prosthodontics, Oral Rehabilitation Program-Araraquara School of Dentistry, UNESP-Univ. Estadual Paulista, Centro, 14801903 Araraquara, SP, Brazil. Electronic address: vergani@foar.unesp.br.
authors.forename: Carlos Eduardo
authors.initials: CE
authors.lastname: Vergani
mesh_terms: D000818:Animals; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D002021:Buffers; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry*; D006531:HEPES / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015603:Keratinocytes / Q000187:drug effects / Q000502:physiology*; D009025:Morpholines / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development*
chemical_list: D002021:Buffers; D003470:Culture Media; D009025:Morpholines; C008550:morpholinopropane sulfonic acid; D006531:HEPES
keywords: Candida albicans; Cell survival; HEPES; Keratinocytes; Staphylococcus aureus