title: Voltammetric detection of glutathione: an adsorptive stripping voltammetry approach.
abstract: A simple, sensitive, and rapid detection of glutathione by cyclic voltammetry using a bare glassy carbon electrode is reported in which glutathione forms a 1 : 1 complex compound with copper(ii) ions. This complex compound is adsorbed onto the electrode surface and undergoes electrochemical oxidation at a characteristic oxidation potential of ca. -0.20 V vs. the standard mercury/mercurous sulphate reference electrode, which is used to detect the glutathione concentration. The linear dynamic range is obtained for a glutathione concentration from 1 μM to 12.5 μM, and the sensitivity is found to be 0.1 ± 0.002 μA μM(-1). A low limit of detection (n = 3) of 0.14 μM and a precision of 1.8% are achieved using a simple, unmodified electrode. The robustness of the present methodology is demonstrated by the successful quantitative analysis of glutathione in the presence of cysteine.
authors.affiliation: Departamento de Química Fundamental, Centro de Ciências Exatas e da Natureza, Universidade Federal de Pernambuco, Av. Jornalista Anibal Fernandes, s/no Cidade Universitária - Recife, PE, Brazil - CEP 50.740-560.
authors.forename: Madalena C C
authors.initials: MC
authors.lastname: Areias
authors.affiliation: Department of Chemistry, Physical and Theoretical Chemistry Laboratory, Oxford University, South Parks Road, Oxford, OX1 3QZ, UK. richard.compton@chem.ox.ac.uk.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Shimizu
authors.affiliation: Department of Chemistry, Physical and Theoretical Chemistry Laboratory, Oxford University, South Parks Road, Oxford, OX1 3QZ, UK. richard.compton@chem.ox.ac.uk.
authors.forename: Richard G
authors.initials: RG
authors.lastname: Compton