title: Three-dimensional culture systems in cancer research: Focus on tumor spheroid model.
abstract: Cancer cells propagated in three-dimensional (3D) culture systems exhibit physiologically relevant cell-cell and cell-matrix interactions, gene expression and signaling pathway profiles, heterogeneity and structural complexity that reflect in vivo tumors. In recent years, development of various 3D models has improved the study of host-tumor interaction and use of high-throughput screening platforms for anti-cancer drug discovery and development. This review attempts to summarize the various 3D culture systems, with an emphasis on the most well characterized and widely applied model - multicellular tumor spheroids. This review also highlights the various techniques to generate tumor spheroids, methods to characterize them, and its applicability in cancer research.
authors.affiliation: Division of Surgical Sciences, Department of Surgery, Duke University School of Medicine, Durham, NC 27710, United States.
authors.forename: Sritama
authors.initials: S
authors.lastname: Nath
authors.affiliation: Division of Surgical Sciences, Department of Surgery, Duke University School of Medicine, Durham, NC 27710, United States; Duke Cancer Institute, Women's Cancer Program, Duke University School of Medicine, Durham, NC 27710, United States. Electronic address: gayathri.devi@duke.edu.
authors.forename: Gayathri R
authors.initials: GR
authors.lastname: Devi
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D002450:Cell Communication / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods*; D002465:Cell Movement / Q000502:physiology; D005434:Flow Cytometry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D009361:Neoplasm Invasiveness / Q000503:physiopathology; D009369:Neoplasms / Q000503:physiopathology*; D018874:Spheroids, Cellular / Q000502:physiology*; D014407:Tumor Cells, Cultured; D059016:Tumor Microenvironment / Q000502:physiology
chemical_list: D000970:Antineoplastic Agents; D020100:Hydrogels
keywords: Apoptosis; High-throughput screening; Inflammatory breast cancer; Invasion; Oxidative stress; Tumor emboli