title: Depot- and gender-specific expression of NLRP3 inflammasome and toll-like receptors in adipose tissue of cancer patients.
abstract: Gender difference in obesity-associated cardiovascular complication could be derived from divergent chronic inflammation. We evaluated depot- and gender-specific regulation of the innate immune system in human adipose tissues. Pair samples were obtained from subcutaneous (SAT) and visceral adipose tissue (VAT) during elective surgery (Male: 35; Female: 27). Expressions of pro- and anti-inflammatory adipocytokines were evaluated by semi-quantitative qPCR. Adipose cell-size distribution was obtained from tissue samples fixed in osmium tetroxide and analyzed by Beckman Coulter Multisizer. Levels of adiponectin were higher in SAT and VAT of female than those of male (P < 0.001 and P = 0.011, respectively). NLRP3, IL1β-IL18, TLR2 were comparable in SAT and VAT between genders. However, TLR4 and TLR9 were increased in female SAT and VAT and HMGB1 in female VAT. Levels of adiponectin were not correlated with mean diameter of adipocyte (φ, μm) in SAT and VAT of male, but negatively well correlated in those of female (r = -0.392 and r = -0.616). Such negative correlations were also observed between levels of TLR2, TLR4, and HMGB1 and φ in female. Levels of NLRP3 and IL1β were positively correlated with φ in male, but not in female. In conclusion, Innate signals were differentially expressed in male and female adipose tissues, suggesting that the depot- and gender-specific signals could be related to gender difference in chronic inflammation. © 2016 BioFactors, 42(4):397-406, 2016.
authors.affiliation: Department of Cardio-Diabetes Medicine, Institute of Biomedical Sciences, University of Tokushima Graduate School, Tokushima, Japan.|Department of Cardiovascular Medicine, Institute of Biomedical Sciences, University of Tokushima Graduate School, Tokushima, Japan.
authors.forename: Michio
authors.initials: M
authors.lastname: Shimabukuro
authors.affiliation: Department of Nutrition and Metabolism, Institute of Biomedical Sciences, University of Tokushima Graduate School, Tokushima, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Sato
authors.affiliation: Department of Urology, Tokushima Prefectural Central Hospital.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Izaki
authors.affiliation: Department of Cardiovascular Medicine, Institute of Biomedical Sciences, University of Tokushima Graduate School, Tokushima, Japan.
authors.forename: Daiju
authors.initials: D
authors.lastname: Fukuda
authors.affiliation: Department of Cardiovascular Medicine, Institute of Biomedical Sciences, University of Tokushima Graduate School, Tokushima, Japan.
authors.forename: Etsuko
authors.initials: E
authors.lastname: Uematsu
authors.affiliation: Department of Pediatrics, Graduate School of Medicine, The University of Tokyo, Japan.
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Hirata
authors.affiliation: Department of Cardiovascular Medicine, Institute of Biomedical Sciences, University of Tokushima Graduate School, Tokushima, Japan.
authors.forename: Shusuke
authors.initials: S
authors.lastname: Yagi
authors.affiliation: Department of Cardiovascular Medicine, Institute of Biomedical Sciences, University of Tokushima Graduate School, Tokushima, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Soeki
authors.affiliation: Department of Nutrition and Metabolism, Institute of Biomedical Sciences, University of Tokushima Graduate School, Tokushima, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sakaue
authors.affiliation: Department of Urology, Institute of Biomedical Sciences, University of Tokushima Graduate School, Tokushima, Japan.
authors.forename: Hiro-Omi
authors.initials: HO
authors.lastname: Kanayama
authors.affiliation: Division of Endocrinology, Diabetes and Metabolism, Hematology, Rheumatology (Second Department of Internal Medicine), Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Masuzaki
authors.affiliation: Department of Cardiovascular Medicine, Institute of Biomedical Sciences, University of Tokushima Graduate School, Tokushima, Japan.
authors.forename: Masataka
authors.initials: M
authors.lastname: Sata
mesh_terms: D052438:Adipocytes, White / Q000473:pathology; D000368:Aged; D048429:Cell Size; D005260:Female; D015870:Gene Expression; D006801:Humans; D058847:Inflammasomes / Q000378:metabolism; D050152:Intra-Abdominal Fat / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D007680:Kidney Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein / Q000378:metabolism*; D009928:Organ Specificity; D010386:Pelvic Neoplasms / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D017678:Sex Distribution; D051193:Toll-Like Receptors / Q000378:metabolism*
chemical_list: D058847:Inflammasomes; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C453881:NLRP3 protein, human; D051193:Toll-Like Receptors
keywords: gender; inflammation; obesity