title: Beef quality with different intramuscular fat content and proteomic analysis using isobaric tag for relative and absolute quantitation of differentially expressed proteins.
abstract: Intramuscular fat (IMF) is an important trait for beef eating quality. The mechanism of how IMF is deposited in beef cattle muscle is not clear at the molecular level. The muscle (M. longissimus lumborum: LL) of a group of Xiangxi yellow×Angus cattle with high fat levels (HF), was compared to the muscle of a low fat group (LF). The meat quality and the expressed protein patterns were compared. It was shown that LL from the HF animals had a greater fat content (P<0.05) and lower moisture content (P<0.05) than LL from LF animals. Forty seven sarcoplasmic proteins were differentially expressed and identified between the two groups. These proteins are involved in 6 molecular functions and 16 biological processes, and affect the Mitogen-activated protein kinases pathway, insulin pathway and c-Jun N-terminal kinases leading to greater IMF deposition. Cattle in the HF group had greater oxidative capacity and lower glycolytic levels suggesting a greater energetic efficiency.
authors.affiliation: College of Food Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, PR China.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Centre for Red Meat and Sheep Development, NSW Department of Primary Industries, Cowra, NSW 2794, Australia.
authors.forename: David L
authors.initials: DL
authors.lastname: Hopkins
authors.affiliation: College of Food Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, PR China.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Food Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: College of Food Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, PR China.
authors.forename: Lixian
authors.initials: L
authors.lastname: Zhu
authors.affiliation: College of Food Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, PR China.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Dong
authors.affiliation: College of Food Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, PR China.
authors.forename: Rongrong
authors.initials: R
authors.lastname: Liang
authors.affiliation: College of Food Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Dai
authors.affiliation: College of Life Science, Shandong Agricultural University, Tai'an, Shandong, PR China. Electronic address: xyunwang@sdau.edu.cn.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, PR China; Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, PR China. Electronic address: luoxin@sdau.edu.cn.
authors.forename: Xin
authors.initials: X
authors.lastname: Luo
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D003296:Cooking; D004041:Dietary Fats / Q000032:analysis*; D063427:Food Quality; D006863:Hydrogen-Ion Concentration; D009124:Muscle Proteins / Q000737:chemistry*; D018482:Muscle, Skeletal / Q000737:chemistry*; D020543:Proteome / Q000737:chemistry; D000069466:Red Meat / Q000032:analysis*; D053719:Tandem Mass Spectrometry; D013696:Temperature
chemical_list: D004041:Dietary Fats; D009124:Muscle Proteins; D020543:Proteome
keywords: Beef; Intramuscular fat; Proteomic