title: CD38 Expression in a Subset of Memory T Cells Is Independent of Cell Cycling as a Correlate of HIV Disease Progression.
abstract: In order to determine if the expression of the activation marker CD38 can correlate with HIV disease progression independently of cycling, we performed a cluster-based multivariate correlation analysis of total circulating CD4(+) T cell counts and viral loads with frequencies of CD38 and Ki67 expression on CD4(+) lymphocytes from patients with untreated HIV infection, stratified in maturation subpopulations, and subpopulation subsets defined by the expression of CXCR5, CXCR3, and CCR4. The frequencies of the activated phenotypes %CD38(+) Ki67(-) and %CD38(+) Ki67(+) of the CXCR5(-) CXCR3(-) CCR4(+) ("pre-Th2") central memory (T(CM)) cell subset clustered together, comprising a significant negative correlate of total circulating CD4(+) T cell counts and a positive correlate of viral load in multivariate analysis. Frequency of cycling-uncoupled CD38 expression in "pre-Th2" T(CM) cells was a negative correlate of total circulating CD4(+) T cell counts in univariate analysis, which was not the case of their %CD38(+) Ki67(+). CXCR5(+) CXCR3(-) CCR4(-) T(CM) cells were underrepresented in patients, and their absolute counts correlated negatively with their %CD38(+) Ki67(-) but not with their % CD38(+) Ki67(+). Our results may imply that CD38 expression either reflects or participates in pathogenic mechanisms of HIV disease independently of cell cycling.
authors.affiliation: Department for Research in Immunology, National Institute for Respiratory Diseases, Calzada de Tlalpan 4502, 14080 Mexico City, DF, Mexico.
authors.forename: Daniela
authors.initials: D
authors.lastname: Würsch
authors.affiliation: Center for Research on Infectious Diseases (CIENI), National Institute for Respiratory Diseases, Calzada de Tlalpan 4502, 14080 Mexico City, DF, Mexico.
authors.forename: Christopher E
authors.initials: CE
authors.lastname: Ormsby
authors.affiliation: Flow Cytometry Core Facility, National Institute for Respiratory Diseases, Calzada de Tlalpan 4502, 14080 Mexico City, DF, Mexico.
authors.forename: Dámaris P
authors.initials: DP
authors.lastname: Romero-Rodríguez
authors.affiliation: Department for Research in Immunology, National Institute for Respiratory Diseases, Calzada de Tlalpan 4502, 14080 Mexico City, DF, Mexico.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Olvera-García
authors.affiliation: Department for Research in Immunology, National Institute for Respiratory Diseases, Calzada de Tlalpan 4502, 14080 Mexico City, DF, Mexico.
authors.forename: Joaquín
authors.initials: J
authors.lastname: Zúñiga
authors.affiliation: Department of Medicine and Department of Immunology and Microbiology, Medical University of South Carolina, 173 Ashley Avenue, The Basic Science Building, Room 208D, Charleston, SC 29425, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Department of Infectious Diseases, National Institute for Medical Sciences and Nutrition, Vasco de Quiroga 15, 14080 Mexico City, DF, Mexico.
authors.forename: Santiago
authors.initials: S
authors.lastname: Pérez-Patrigeon
authors.affiliation: Department for Research in Immunology, National Institute for Respiratory Diseases, Calzada de Tlalpan 4502, 14080 Mexico City, DF, Mexico.
authors.forename: Enrique
authors.identifier: 0000-0002-3231-1626
authors.initials: E
authors.lastname: Espinosa
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000378:metabolism*; D000328:Adult; D018791:CD4 Lymphocyte Count; D002453:Cell Cycle; D018450:Disease Progression; D005260:Female; D015658:HIV Infections / Q000276:immunology / Q000378:metabolism* / Q000473:pathology* / Q000821:virology; D015497:HIV-1 / Q000502:physiology; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism*; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008875:Middle Aged; D019562:Viral Load
chemical_list: D019394:Ki-67 Antigen; D008562:Membrane Glycoproteins; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1