title: Green synthesis of Se/Ru alloy nanoparticles using gallic acid and evaluation of theiranti-invasive effects in HeLa cells.
abstract: Methods for the synthesis of nanoparticles (NPs) for biomedical applications ideally involve the use of nontoxic reducing and capping agents, and more importantly, enable control over the shape and size of the particles. As such, we used gallic acid (GA) as both a reducing and a capping agent in a simple and "green" synthesis of stable Se/Rualloy NPs (GA-Se/RuNPs). The diameter and morphology of the Se/Ru alloy NPs were regulated by GA concentration, and the presence of Ru was found to be a key factor in regulating and controlling the size of GA-Se/RuNPs. Moreover, GA-Se/RuNPs suppressed HeLa cell proliferation through the induction of apoptosis at concentrations that were nontoxic in normal cells. Furthermore, GA-Se/RuNPs effectively inhibited migration and invasion in HeLa cells via the inhibition of MMP-2 and MMP-9 proteins. Our findings confirm that bimetallic (Se/Ru) NPs prepared via GA-mediated synthesis exhibit enhanced anticancer effects.
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Xu
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, China.
authors.forename: Yuqian
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, China. Electronic address: tliuliu@jnu.edu.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, China; School of Pharmacy, Guangdong Medical University, Zhanjiang 524023, China. Electronic address: lanmeichen@126.com.
authors.forename: Lanmei
authors.initials: L
authors.lastname: Chen
mesh_terms: D000497:Alloys; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005707:Gallic Acid / Q000494:pharmacology*; D055772:Green Chemistry Technology / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009361:Neoplasm Invasiveness; D010316:Particle Size; D012428:Ruthenium / Q000737:chemistry*; D012643:Selenium / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D000497:Alloys; D000970:Antineoplastic Agents; D005707:Gallic Acid; D012428:Ruthenium; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D012643:Selenium
keywords: Anti-invasive properties; Apoptosis; Gallic acid; Se/Ru alloy nanoparticles; Selenium nanoparticles