title: [1-8-NαC]-Zanriorb A1, a Proapoptotic Orbitide from Leaves of Zanthoxylum riedelianum.
abstract: A new orbitide named [1-8-NαC]-zanriorb A1 (1) was isolated and characterized from the leaves of Zanthoxylum riedelianum using NMR and mass spectrometry. The absolute configuration of the amino acids was determined using Marfey's method on the acid hydrolysates. Compound 1 induced cell death by apoptosis in Jurkat leukemia T cells (IC50 218 nM).
authors.affiliation: Departamento de Química, Universidade Federal de Mato Grosso , Cuiabá, MT 78060-900, Brazil.
authors.forename: Pâmela J
authors.initials: PJ
authors.lastname: Dos S Beirigo
authors.affiliation: Departamento de Bioquímica, Universidade Federal de São Paulo , São Paulo, SP 04039-032, Brazil.
authors.forename: Heron F V
authors.initials: HF
authors.lastname: Torquato
authors.affiliation: Departamento de Química, Universidade Federal Rural do Rio de Janeiro , Seropédica, RJ 23890-000, Brazil.
authors.forename: Carlos H C
authors.initials: CH
authors.lastname: Dos Santos
authors.affiliation: Departamento de Química, Universidade Federal Rural do Rio de Janeiro , Seropédica, RJ 23890-000, Brazil.
authors.forename: Mário G de
authors.initials: MG
authors.lastname: Carvalho
authors.affiliation: Departamento de Química, Universidade Federal Rural do Rio de Janeiro , Seropédica, RJ 23890-000, Brazil.
authors.forename: Rosane N
authors.initials: RN
authors.lastname: Castro
authors.affiliation: Departamento de Bioquímica, Universidade Federal de São Paulo , São Paulo, SP 04039-032, Brazil.
authors.forename: Edgar J
authors.initials: EJ
authors.lastname: Paredes-Gamero
authors.affiliation: Departamento de Química, Universidade Federal de Mato Grosso , Cuiabá, MT 78060-900, Brazil.
authors.forename: Paulo T
authors.initials: PT
authors.lastname: de Sousa
authors.affiliation: Departamento de Química, Universidade Federal de Mato Grosso , Cuiabá, MT 78060-900, Brazil.
authors.forename: Marcos J
authors.initials: MJ
authors.lastname: Jacinto
authors.affiliation: Departamento de Química, Universidade Federal de Mato Grosso , Cuiabá, MT 78060-900, Brazil.
authors.forename: Virgínia C
authors.initials: VC
authors.lastname: da Silva
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001938:Brazil; D004247:DNA / Q000032:analysis; D006801:Humans; D019169:Jurkat Cells; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*; D032106:Zanthoxylum / Q000737:chemistry*
chemical_list: C000619771:(1-8-NαC)-zanriorb A1; D010456:Peptides, Cyclic; D004247:DNA