title: Non-enzymatic electrochemical biosensor based on Pt NPs/RGO-CS-Fc nano-hybrids for the detection of hydrogen peroxide in living cells.
abstract: A highly sensitive non-enzymatic electrochemical sensor based on platinum nanoparticles/reduced graphene oxide-chitosan-ferrocene carboxylic acid nano-hybrids (Pt NPs/RGO-CS-Fc biosensor) was developed for the measurement of hydrogen peroxide (H2O2). The RGO-CS-Fc nano-hybrids was prepared and characterized by UV-vis spectrum, Fourier transform infrared spectroscopy, transmission electron microscopy, Raman spectrometer and electrochemical impedance spectroscopy. Under optimal experimental conditions, the Pt NPs/RGO-CS-Fc biosensor showed outstanding catalytic activity toward H2O2 reduction. The current response of the biosensor presented a linear relationship with H2O2 concentration from 2.0×10(-8)M to 3.0×10(-6)M with a correlation coefficient of R(2)=0.9968 and with logarithm of H2O2 concentration from 6.0×10(-6)M to 1.0×10(-2)M with a correlation coefficient of R(2)=0.9887, the low detection limit of 20nM was obtained at the signal/noise (S/N) ratio of 3. Moreover, the Pt NPs/RGO-CS-Fc biosensor exhibited excellent anti-interference capability and reproducibility for the detection of H2O2. The biosensor was also successfully applied for the detection of H2O2 from living cells containing normal and cancer cells. All these results prove that the Pt NPs/RGO-CS-Fc biosensor has the potential application in clinical diagnostics to evaluate oxidative stress of different living cells.
authors.affiliation: School of Life and Environment Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China.
authors.forename: Zhihao
authors.initials: Z
authors.lastname: Bai
authors.affiliation: School of Life and Environment Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China; National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Nanning, Guangxi 530021, China.
authors.forename: Guiyin
authors.initials: G
authors.lastname: Li
authors.affiliation: School of Life and Environment Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China.
authors.forename: Jingtao
authors.initials: J
authors.lastname: Liang
authors.affiliation: National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Nanning, Guangxi 530021, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Su
authors.affiliation: Guangxi Key Laboratory of Metabolic Diseases Research, Guilin 181st Hospital, Guilin, Guangxi 541002, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Guangxi Key Laboratory of Metabolic Diseases Research, Guilin 181st Hospital, Guilin, Guangxi 541002, China.
authors.forename: Huaizhou
authors.initials: H
authors.lastname: Chen
authors.affiliation: School of Life and Environment Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China; National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Nanning, Guangxi 530021, China. Electronic address: huangyong503@126.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Guangxi Key Laboratory of Metabolic Diseases Research, Guilin 181st Hospital, Guilin, Guangxi 541002, China. Electronic address: suiwg@163.com.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Sui
authors.affiliation: National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Nanning, Guangxi 530021, China. Electronic address: yongxiang_zhao@126.com.
authors.forename: Yongxiang
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D002460:Cell Line; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D005296:Ferrous Compounds / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D000075163:Metallocenes; D010084:Oxidation-Reduction; D010984:Platinum / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D005296:Ferrous Compounds; D000075163:Metallocenes; C032949:ferrocenecarboxylic acid; D010984:Platinum; D006108:Graphite; D048271:Chitosan; D006861:Hydrogen Peroxide
keywords: Ferrocene carboxylic acid; Graphene; Hydrogen peroxide; Non-enzymatic sensor; Platinum nanoparticles