title: T-cell factor (TCF/LEF1) binding elements (TBEs) of FasL (Fas ligand or CD95 ligand) bind and cluster Fas (CD95) and form complexes with the TCF-4 and b-catenin transcription factors in vitro and in vivo which result in triggering cell death and/or cell activation.
abstract: T-cell factor 4 (TCF4) is an important transcription factor of the Wnt signaling system. β-catenin, an upstream protein of TCF4, accumulates in the cytoplasm, then translocates to the nucleus to activate the β-catenin/T-cell factor/lymphoid enhancer factor (TCF/LEF) transcriptional machinery and regulates target genes. Previous studies showed that TCF4 was involved in cell proliferation and apoptosis. However, its expression and function in central nervous system injury are unclear. We performed a traumatic brain injury (TBI) model in adult rats. The expression of TCF4 in the brain cortex detected by Western blot increased after TBI. Double immunofluorescence staining revealed that TCF4 was expressed by neurons and microglia. In addition, co-localization of TCF4 with active caspase-3 or proliferating cell nuclear antigen was observed in neurons and microglia, respectively, suggesting that TCF4 might participate in neuronal apoptosis and microglial proliferation after TBI. To further investigate the functions of TCF4, PC12 and HAPI cells were employed to establish a neuronal apoptosis and microglial proliferation model in vitro, respectively. Knocking down TCF4 with siRNA proved the pro-apoptotic and pro-proliferation effect of TCF4 in PC12 and HAPI cells, respectively. Taken together, TCF4 might promote neuronal apoptosis and microglial proliferation after TBI.
authors.affiliation: Department of Pathophysiology, Medical College, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Nautical Medicine, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Affiliated Hospital of Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Li
authors.affiliation: Laboratory Animal Center, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.|Department of Pathogen Biology, Medical College, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Chun
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Osteology, The Second Affiliated Hospital, Nantong University, Nantong, 226001, People's Republic of China.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Shen
authors.initials: S
authors.lastname: Huang
authors.affiliation: Department of Neurosurgery, Wuxi Second Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu, 214002, People's Republic of China.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Dezhi
authors.initials: D
authors.lastname: Xu
authors.affiliation: Institute of Nautical Medicine, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China. wy@ntu.edu.cn.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Pathogen Biology, Medical College, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China. ntlxj0305@163.com.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China. ntlxj0305@163.com.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D000070642:Brain Injuries, Traumatic / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism; D016923:Cell Death; D053222:Fas Ligand Protein / Q000378:metabolism; D008297:Male; D016716:PC12 Cells; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D058118:Transcription Factor 7-Like 2 Protein / Q000378:metabolism*; D060449:Wnt Signaling Pathway / Q000502:physiology; D051176:beta Catenin / Q000378:metabolism
chemical_list: C495268:Ctnnb1 protein, rat; D053222:Fas Ligand Protein; D058118:Transcription Factor 7-Like 2 Protein; D051176:beta Catenin; C505410:Casp3 protein, rat; D053148:Caspase 3
keywords: Apoptosis; Cell cycle protein; Proliferation; Rat; T-cell factor 4 (TCF4); Traumatic brain injury