title: Mast Cell-Derived Exosomes Promote Th2 Cell Differentiation via OX40L-OX40 Ligation.
abstract: Exosomes are nanovesicles released by different cell types, such as dendritic cells (DCs), mast cells (MCs), and tumor cells. Exosomes of different origin play a role in antigen presentation and modulation of immune response to infectious disease. In this study, we demonstrate that mast cells and CD4(+) T cells colocated in peritoneal lymph nodes from BALB/c mouse. Further, bone marrow-derived mast cells (BMMCs) constitutively release exosomes, which express CD63 and OX40L. BMMC-exosomes partially promoted the proliferation of CD4(+) T cells. BMMC-exosomes significantly enhanced the differentiation of naive CD4(+) T cells to Th2 cells in a surface contact method, and this ability was partly inhibited by the addition of anti-OX40L Ab. In conclusion, BMMC-exosomes promoted the proliferation and differentiation of Th2 cells via ligation of OX40L and OX40 between exosomes and T cells. This method represents a novel mechanism, in addition to direct cell surface contacts, soluble mediators, and synapses, to regulate T cell actions by BMMC-exosomes.
authors.affiliation: Department of Laboratory Medicine, Shanghai First People's Hospital, Shanghai Jiao Tong University School, Shanghai 200080, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Laboratory Medicine, Shanghai First People's Hospital, Shanghai Jiao Tong University School, Shanghai 200080, China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, Shanghai First People's Hospital, Shanghai Jiao Tong University School, Shanghai 200080, China.
authors.forename: Lihui
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Laboratory Medicine, Shanghai First People's Hospital, Shanghai Jiao Tong University School, Shanghai 200080, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, Shanghai First People's Hospital, Shanghai Jiao Tong University School, Shanghai 200080, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xiao
authors.affiliation: Department of Laboratory Medicine, Shanghai First People's Hospital, Shanghai Jiao Tong University School, Shanghai 200080, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Laboratory Medicine, Shanghai First People's Hospital, Shanghai Jiao Tong University School, Shanghai 200080, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Peng
authors.affiliation: Department of Laboratory Medicine, Shanghai First People's Hospital, Shanghai Jiao Tong University School, Shanghai 200080, China.
authors.forename: Huirong
authors.initials: H
authors.lastname: Dai
authors.affiliation: Department of Laboratory Medicine, Shanghai First People's Hospital, Shanghai Jiao Tong University School, Shanghai 200080, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D001854:Bone Marrow Cells / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry / Q000276:immunology; D002454:Cell Differentiation; D018920:Coculture Techniques; D055354:Exosomes / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000648:ultrastructure; D008198:Lymph Nodes / Q000276:immunology; D008407:Mast Cells / Q000166:cytology / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D053278:OX40 Ligand / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010537:Peritoneum / Q000166:cytology / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology*
chemical_list: D053278:OX40 Ligand