title: Contamination of beef products with staphylococcal classical enterotoxins in Egypt and Saudi Arabia.
abstract: Food-borne pathogens are of high concern for public health and food safety. Staphylococcus aureus food poisoning is one of the most economically devastating types of food poisoning globally. The purpose of this study was to detect staphylococcal classical enterotoxins (SEs) in processed beef from Kingdom of Saudi Arabia (KSA) and Egypt. In the present investigation a total of 250 random processed meat samples (50 each of minced meat, beef burger, beef sausage, beef kofta and beef luncheon) were collected from different super markets in the study area. Using conventional cultural methods, samples were cultured for isolation and identification of S. aureus. Multiplex PCR was used to detect SEs of the classical type SEA, SEB, SEC and SED from isolates. The percentage presence of S. aureus in minced meat, beef burger, beef sausage, beef kofta and beef luncheon was 38%, 22%, 30%, 32% and 12%, respectively. Multiplex PCR indicated that all examined samples contain different types of classical staphylococcal enterotoxins and only minced meat samples contained all four types of toxins. Multiplex PCR is efficient in detection of SEs from food and may be used in tracing of toxins to promote food hygiene. Implications of contamination of processed meat to food hygiene in the study area are highlighted.
authors.affiliation: Department of Food Hygiene & Control, Faculty of Veterinary Medicine, University of Sadat City, Egypt.
authors.forename: Reyad R
authors.initials: RR
authors.lastname: Shawish
authors.affiliation: Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, King Faisal University, Al-Ahsa, Kingdom of Saudi Arabia.
authors.forename: Naser A
authors.initials: NA
authors.lastname: Al-Humam
keywords: Egypt; Kingdom of Saudi Arabia; PCR; Staphylococcus aureus; meat products; staphylococcal enterotoxins