title: Sound exposure changes European seabass behaviour in a large outdoor floating pen: Effects of temporal structure and a ramp-up procedure.
abstract: Underwater sound from human activities may affect fish behaviour negatively and threaten the stability of fish stocks. However, some fundamental understanding is still lacking for adequate impact assessments and potential mitigation strategies. For example, little is known about the potential contribution of the temporal features of sound, the efficacy of ramp-up procedures, and the generalisability of results from indoor studies to the outdoors. Using a semi-natural set-up, we exposed European seabass in an outdoor pen to four treatments: 1) continuous sound, 2) intermittent sound with a regular repetition interval, 3) irregular repetition intervals and 4) a regular repetition interval with amplitude 'ramp-up'. Upon sound exposure, the fish increased swimming speed and depth, and swam away from the sound source. The behavioural readouts were generally consistent with earlier indoor experiments, but the changes and recovery were more variable and were not significantly influenced by sound intermittency and interval regularity. In addition, the 'ramp-up' procedure elicited immediate diving response, similar to the onset of treatment without a 'ramp-up', but the fish did not swim away from the sound source as expected. Our findings suggest that while sound impact studies outdoors increase ecological and behavioural validity, the inherently higher variability also reduces resolution that may be counteracted by increasing sample size or looking into different individual coping styles. Our results also question the efficacy of 'ramp-up' in deterring marine animals, which warrants more investigation.
authors.affiliation: Behavioural Biology, Institute of Biology Leiden (IBL), Leiden University, The Netherlands. Electronic address: y.y.neo.2@umail.leidenuniv.nl.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Neo
authors.affiliation: Behavioural Biology, Institute of Biology Leiden (IBL), Leiden University, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Hubert
authors.affiliation: Institute for Marine Resources & Ecosystem Studies (IMARES) IJmuiden, Wageningen UR, The Netherlands.
authors.forename: L
authors.initials: L
authors.lastname: Bolle
authors.affiliation: Institute for Marine Resources & Ecosystem Studies (IMARES) IJmuiden, Wageningen UR, The Netherlands.
authors.forename: H V
authors.initials: HV
authors.lastname: Winter
authors.affiliation: Behavioural Biology, Institute of Biology Leiden (IBL), Leiden University, The Netherlands.
authors.forename: C
authors.initials: C
authors.lastname: Ten Cate
authors.affiliation: Behavioural Biology, Institute of Biology Leiden (IBL), Leiden University, The Netherlands.
authors.forename: H
authors.initials: H
authors.lastname: Slabbekoorn
mesh_terms: D000818:Animals; D001492:Bass / Q000502:physiology*; D001522:Behavior, Animal; D004781:Environmental Exposure; D005260:Female; D006801:Humans; D008297:Male; D013016:Sound; D013550:Swimming
keywords: Anthropogenic noise; Dicentrarchus labrax; Field study; Fish behaviour; Sound characteristics; Stress response