title: High-efficiency cellular reprogramming with microfluidics.
abstract: We report that the efficiency of reprogramming human somatic cells to induced pluripotent stem cells (hiPSCs) can be dramatically improved in a microfluidic environment. Microliter-volume confinement resulted in a 50-fold increase in efficiency over traditional reprogramming by delivery of synthetic mRNAs encoding transcription factors. In these small volumes, extracellular components of the TGF-β and other signaling pathways exhibited temporal regulation that appears critical to acquisition of pluripotency. The high quality and purity of the resulting hiPSCs (μ-hiPSCs) allowed direct differentiation into functional hepatocyte- and cardiomyocyte-like cells in the same platform without additional expansion.
authors.affiliation: Department of Industrial Engineering, University of Padova, Padova, Italy.|Venetian Institute of Molecular Medicine (VIMM), Padova, Italy.
authors.forename: Camilla
authors.identifier: 0000-0002-1211-9629
authors.initials: C
authors.lastname: Luni
authors.affiliation: Department of Industrial Engineering, University of Padova, Padova, Italy.|Venetian Institute of Molecular Medicine (VIMM), Padova, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Giulitti
authors.affiliation: Department of Industrial Engineering, University of Padova, Padova, Italy.|Venetian Institute of Molecular Medicine (VIMM), Padova, Italy.
authors.forename: Elena
authors.identifier: 0000-0001-7990-8852
authors.initials: E
authors.lastname: Serena
authors.affiliation: Department of Industrial Engineering, University of Padova, Padova, Italy.|Venetian Institute of Molecular Medicine (VIMM), Padova, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Ferrari
authors.affiliation: Department of Industrial Engineering, University of Padova, Padova, Italy.|Venetian Institute of Molecular Medicine (VIMM), Padova, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Zambon
authors.affiliation: Department of Industrial Engineering, University of Padova, Padova, Italy.|Venetian Institute of Molecular Medicine (VIMM), Padova, Italy.
authors.forename: Onelia
authors.initials: O
authors.lastname: Gagliano
authors.affiliation: Department of Industrial Engineering, University of Padova, Padova, Italy.|Venetian Institute of Molecular Medicine (VIMM), Padova, Italy.
authors.forename: Giovanni G
authors.initials: GG
authors.lastname: Giobbe
authors.affiliation: Department of Industrial Engineering, University of Padova, Padova, Italy.|Venetian Institute of Molecular Medicine (VIMM), Padova, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Michielin
authors.affiliation: Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Knöbel
authors.affiliation: Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Bosio
authors.affiliation: Department of Industrial Engineering, University of Padova, Padova, Italy.|Venetian Institute of Molecular Medicine (VIMM), Padova, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Elvassore
mesh_terms: D002478:Cells, Cultured; D065150:Cellular Reprogramming / Q000235:genetics*; D000067470:Cellular Reprogramming Techniques / Q000379:methods*; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D044085:Microfluidics / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D012333:RNA, Messenger