title: Human bronchial and parenchymal fibroblasts display differences in basal inflammatory phenotype and response to IL-17A.
abstract: Chronic inflammation, typified by increased expression of IL-17A, together with airway and parenchymal remodelling are features of chronic lung diseases. Emerging evidence suggests that phenotypic heterogeneity of repair and inflammatory capacities of fibroblasts may contribute to the differential structural changes observed in different regions of the lung.
  
  
  
  To investigate phenotypic differences in parenchymal and bronchial fibroblasts, either in terms of inflammation and remodelling or the ability of these fibroblasts to respond to IL-17A.
  
  
  
  Four groups of primary fibroblasts were used: normal human bronchial fibroblast (NHBF), normal human parenchymal fibroblast (NHPF), COPD human bronchial fibroblast (CHBF) and COPD human parenchymal fibroblast (CHPF). Cytokine and extracellular matrix (ECM) expression were measured at baseline and after stimulation with IL-17A. Actinomycin D was used to measure cytokine mRNA stability.
  
  
  
  At baseline, we observed higher protein production of IL-6 in NHPF than NHBF, but higher levels of IL-8 and GRO-α in NHBF. IL-17A induced a higher expression of GRO-α (CXCL1) and IL-6 in NHPF than in NHBF, and a higher level of IL-8 expression in NHBF. IL-17A treatment decreased the mRNA stability of IL-6 in NHBF when compared with NHPF. CHPF expressed higher protein levels of fibronectin, collagen-I and collagen-III than CHBF, NHBF and NHPF. IL-17A increased fibronectin and collagen-III protein only in NHPF and collagen-III protein production in CHBF and CHPF.
  
  
  
  These findings provide insight into the inflammatory and remodelling processes that may be related to the phenotypic heterogeneity of fibroblasts from airway and parenchymal regions and in their response to IL-17A.
authors.affiliation: Meakins-Christie Laboratories, McGill University, Montreal, QC, Canada.
authors.forename: K
authors.initials: K
authors.lastname: Dessalle
authors.affiliation: Meakins-Christie Laboratories, McGill University, Montreal, QC, Canada.
authors.forename: V
authors.initials: V
authors.lastname: Narayanan
authors.affiliation: Meakins-Christie Laboratories, McGill University, Montreal, QC, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Kyoh
authors.affiliation: Meakins-Christie Laboratories, McGill University, Montreal, QC, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Mogas
authors.affiliation: Faculty of Medicine, University of Manitoba, Winnipeg, MB, Canada.
authors.forename: A J
authors.initials: AJ
authors.lastname: Halayko
authors.affiliation: Firestone Institute for Respiratory Health, St. Joseph's Healthcare and Department of Medicine, McMaster University, Hamilton, ON, Canada.
authors.forename: P
authors.initials: P
authors.lastname: Nair
authors.affiliation: Meakins-Christie Laboratories, McGill University, Montreal, QC, Canada.
authors.forename: C J
authors.initials: CJ
authors.lastname: Baglole
authors.affiliation: Meakins-Christie Laboratories, McGill University, Montreal, QC, Canada.
authors.forename: D H
authors.initials: DH
authors.lastname: Eidelman
authors.affiliation: Meakins-Christie Laboratories, McGill University, Montreal, QC, Canada.
authors.forename: M S
authors.initials: MS
authors.lastname: Ludwig
authors.affiliation: Meakins-Christie Laboratories, McGill University, Montreal, QC, Canada.|College of Medicine, University of Sharjah, UAE.
authors.forename: Q
authors.initials: Q
authors.lastname: Hamid
mesh_terms: D001980:Bronchi / Q000166:cytology / Q000378:metabolism*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005109:Extracellular Matrix; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D007249:Inflammation / Q000209:etiology* / Q000378:metabolism*; D020381:Interleukin-17 / Q000378:metabolism* / Q000494:pharmacology; D000072356:Parenchymal Tissue / Q000166:cytology / Q000378:metabolism*; D029424:Pulmonary Disease, Chronic Obstructive / Q000209:etiology / Q000378:metabolism; D020871:RNA Stability; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D016207:Cytokines; D020381:Interleukin-17; D012333:RNA, Messenger; D011994:Recombinant Proteins
keywords: IL-17A; cytokines; extracellular matrix; fibroblasts; heterogeneity