title: The atypical cadherin Dachsous1 localizes to the base of the ciliary apparatus in airway epithelia.
abstract: Mucociliary clearance requires the distinct orientation and coordinated movement of airway cilia, which is established through planar cell polarity signaling (PCP). The atypical cadherin Dachsous1 (Dchs1) is a transmembrane protein that regulates PCP in D. melanogaster. However, little is known about Dchs1 expression and its potential role in PCP in mammalian adult tissues. Here, we show that Dchs1 is ubiquitously expressed in mouse embryos, but exhibits a highly restricted expression to lung tissues in the adult stage. Strikingly, human Dchs1 localized exclusively to the base of the ciliary apparatus in cultured human respiratory epithelial cells with differentiated motile 9 + 2 cilia. This localization could be functionally important as we observed aberrant DCHS1 mRNA expression in human non-small cell lung cancer tissue. In sum, we establish Dchs1 as a component of the membrane domain surrounding the ciliary base. This suggests a specific role of Dchs1 in PCP-dependent organization of ciliary function and a possible role in lung disease.
authors.affiliation: Gottfried von Preyer'sches Kinderspital des Kaiser-Franz-Josef-Spitals, Vienna, Austria. Electronic address: cecile.dau@wienkav.at.
authors.forename: Cécile
authors.initials: C
authors.lastname: Dau
authors.affiliation: Center for Chronic Immunodeficiency (CCI), University Medical Center Freiburg and University of Freiburg, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: Fliegauf
authors.affiliation: Department of Pediatrics, Universitätsklinikum Münster, Germany.
authors.forename: Heymut
authors.initials: H
authors.lastname: Omran
authors.affiliation: Institute of Pathology, RWTH Aachen University, Aachen, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Schlensog
authors.affiliation: Institute of Pathology, RWTH Aachen University, Aachen, Germany.
authors.forename: Edgar
authors.initials: E
authors.lastname: Dahl
authors.affiliation: Division of Nephrology and Immunology, RWTH Aachen University, Aachen, Germany.
authors.forename: Claudia R
authors.initials: CR
authors.lastname: van Roeyen
authors.affiliation: Institute for Neuroanatomy, Medical Faculty Mannheim of the University of Heidelberg, Germany.
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Kriz
authors.affiliation: Division of Nephrology and Immunology, RWTH Aachen University, Aachen, Germany.
authors.forename: Marcus J
authors.initials: MJ
authors.lastname: Moeller
authors.affiliation: Division of Nephrology and Immunology, RWTH Aachen University, Aachen, Germany.
authors.forename: Gerald S
authors.initials: GS
authors.lastname: Braun
mesh_terms: D000375:Aging / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D000070585:Cadherin Related Proteins; D015820:Cadherins / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000473:pathology; D002923:Cilia / Q000378:metabolism* / Q000473:pathology; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D020545:Respiratory Mucosa / Q000378:metabolism* / Q000473:pathology; D013347:Subcellular Fractions / Q000378:metabolism / Q000473:pathology; D014018:Tissue Distribution
chemical_list: D000070585:Cadherin Related Proteins; D015820:Cadherins; C442479:DCHS1 protein, human
keywords: Basal body; Dachsous1; Lung cancer; Planar cell polarity; Respiratory epithelial cell