title: N-Glycopeptide Profiling in Arabidopsis Inflorescence.
abstract: This study presents the first large-scale analysis of plant intact glycopeptides. Using wheat germ agglutinin lectin weak affinity chromatography to enrich modified peptides, followed by electron transfer dissociation (ETD)(1) fragmentation tandem mass spectrometry, glycan compositions on over 1100 glycopeptides from 270 proteins found in Arabidopsis inflorescence tissue were characterized. While some sites were only detected with a single glycan attached, others displayed up to 16 different glycoforms. Among the identified glycopeptides were four modified in nonconsensus glycosylation motifs. While most of the modified proteins are secreted, membrane, endoplasmic reticulum (ER), or Golgi-localized proteins, surprisingly, N-linked sugars were detected on a protein predicted to be cytosolic or nuclear.
authors.affiliation: From the ‡Department of Plant Biology, Carnegie Institution for Science, Stanford, California 94305; §Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California 94143.
authors.forename: Shou-Ling
authors.initials: SL
authors.lastname: Xu
authors.affiliation: §Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California 94143.
authors.forename: Katalin F
authors.initials: KF
authors.lastname: Medzihradszky
authors.affiliation: From the ‡Department of Plant Biology, Carnegie Institution for Science, Stanford, California 94305;
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Wang
authors.affiliation: §Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California 94143.
authors.forename: Alma L
authors.initials: AL
authors.lastname: Burlingame
authors.affiliation: §Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California 94143 chalkley@cgl.ucsf.edu.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Chalkley
mesh_terms: D017360:Arabidopsis / Q000737:chemistry* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002853:Chromatography, Liquid; D003600:Cytosol / Q000378:metabolism; D006020:Glycopeptides / Q000032:analysis*; D006031:Glycosylation; D056626:Inflorescence / Q000737:chemistry*; D011499:Protein Processing, Post-Translational; D053719:Tandem Mass Spectrometry
chemical_list: D029681:Arabidopsis Proteins; D006020:Glycopeptides