title: Specific receptor for hydrazine: mapping the in situ release of hydrazine in live cells and in an in vitro enzymatic assay.
abstract: We report a new chemodosimetric reagent capable of detecting hydrazine in the presence of several other competing amine derivatives and ionic analytes of biological relevance. This reagent has been utilized for real time monitoring of in situ N2H4 release during the metabolism of a crucial tuberculosis drug, isoniazid, in live HepG2 cells. The fluorescence response of the reagent based on its specific reaction with N2H4 is used for developing an in vitro assay for aminoacylase-1.
authors.affiliation: Organic Chemistry Division, CSIR-National Chemical Laboratory, Pune-411008, India.
authors.forename: Firoj
authors.initials: F
authors.lastname: Ali
authors.forename: Anila H
authors.initials: AH
authors.lastname: A
authors.forename: Nandaraj
authors.initials: N
authors.lastname: Taye
authors.forename: Devraj G
authors.initials: DG
authors.lastname: Mogare
authors.forename: Samit
authors.initials: S
authors.lastname: Chattopadhyay
authors.forename: Amitava
authors.initials: A
authors.lastname: Das
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D057075:Enzyme Assays; D045325:HCT116 Cells; D056945:Hep G2 Cells; D006801:Humans; D006834:Hydrazines / Q000737:chemistry / Q000378:metabolism*; D007538:Isoniazid / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D008163:Luminescent Measurements; D018613:Microscopy, Confocal; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D006834:Hydrazines; C029424:hydrazine; D012822:Silicon Dioxide; D000581:Amidohydrolases; C019672:aminoacylase I; D007538:Isoniazid