title: [The relationship between the effects of MTA on mRNA expression of four iconic proteins in cells of fetal rat skull and cell culture environment].
abstract: OBJECTIVE
  To discuss the influence of MTA on mRNA expression of Cbfa1, ALP, Col-Ⅰand BGP which are 4 kinds of iconic protein in cells of fetal rats skull, and explore its influence on cell culture environment and association of changes of calcium, phosphorus.
  
  
  METHODS
  Cells were obtained by 2 kinds of mixed enzymatic digestion for 3 steps from gestation fetal rat calvarial bone. The expression of Cbfa1mRNA, ALPmRNA, Col-1mRNA, BGP mRNA and extracellular calcium were detected. Phosphorus (P) and calcium concentration of fetal rat skull cells co-cultured with MTA for 3 weeks at different stages of cell differentiation was assessed atomic absorption spectrophotometry. The data was statistically analyzed using SPSS10.0 software package.
  
  
  RESULTS
  At the 4th day, P3- content decreased significantly (P<0.05), while ALPmRNA in MTA group increased most greatly and was 40 times of the control group. At the 14th to 18th day, the Ca2+ and P3- content reduced significantly (P<0.05), and then the BGPmRNA in MTA group rised most greatly which was about 7.71 times of the control group. Then Cbfalpha l mRNA in MTA group increased most strongly later which was about 7.38 times of the control group. Col Ⅰ mRNA increased minimally in all time points.
  
  
  CONCLUSIONS
  The change of P3- content may be the initiating factor when MTA promoted differentiation of fetal rats skull cells in vitro, and Ca2+ could greatly accelerate the process of mineralization when accumulated to a certain extent. At the same time, the expression of ALPmRNA, BGPmRNA, Col Ⅰ mRNA and Cbfalpha lmRNA were regulated accordingly, which is the key to explain osteogenetic mechanism of MTA.
authors.affiliation: Department of Dentistry, Shiyan People's Hospital,Affiliated to Hubei Medical College. Shiyan 442000, Hubei Province, China. E-mail:weiweig5yy@163.com.
authors.forename: Jun-Yuan
authors.initials: JY
authors.lastname: Zheng
authors.affiliation: Department of Dentistry, Shiyan People's Hospital,Affiliated to Hubei Medical College. Shiyan 442000, Hubei Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: He
authors.affiliation: Department of Dentistry, Shiyan People's Hospital,Affiliated to Hubei Medical College. Shiyan 442000, Hubei Province, China.
authors.forename: Tu-qiang
authors.initials: TQ
authors.lastname: Hu
mesh_terms: D000818:Animals; D002118:Calcium; D017610:Calcium Compounds; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects*; D000068437:Pemetrexed / Q000494:pharmacology; D011506:Proteins; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012886:Skull
chemical_list: D017610:Calcium Compounds; D011506:Proteins; D012333:RNA, Messenger; D000068437:Pemetrexed; D002118:Calcium