title: CHARACTERIZATION AND EXPRESSION PROFILES OF FIVE POSSIBLE CYTOCHROME P450 GENES FROM Liposcelis entomophila (ENDERLEIN) (PSOCOPTERA: LIPOSCELIDIDAE).
abstract: In this study, the cDNAs of five cytochromes P450 genes (named CYP345P1, CYP358B1, CYP4FD2, CYP4CD2, and CYP6JN1) contained open reading frames from 1,500 to 1,554 nucleotides that encoded 499 to 517 amino acids were cloned from the psocid Liposcelis entomophila. They are characterized by predicted molecular weights from 57.67 to 59.64 kDa and theoretical isoelectric points of 5.57-9.07. Quantitative real-time PCR analysis showed these five genes were expressed at all tested developmental stages and higher expressions were observed in adults. CYP358B1 was expressed at higher levels in egg and adult compared to the larval stages. mRNA abundances of five genes were detected in both sexes and were relatively more abundant in adult females than in adult males. Synergism bioassay showed that the synergic ratio was 2.20 and 2.45 when insects were treated with the mixture of deltamethrin or malathion with the synergist piperonyl butoxide (PBO). Because PBO induces cytochrome P450s in some insects, this suggested to us that cytochromes P450 might participate in detoxification of these insecticides. The transcripts of the five cytochromes P450 genes in adult psocids could be induced to the highest level at 12 h after the exposure to malathion. After exposure to deltamethrin, CYP358B1 reached maximum expression at 24 h. The maximum expression of the other four genes occurred at 36 h. Treatments with the carbamate propoxur did not influence transcription of the cytochromes P450 gene. The induction profiles suggested that these five cytochrome P450 genes may be associated with deltamethrin and malathion metabolism in psocids.
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, P. R. China.
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, P. R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, P. R. China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Wei
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, P. R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Shang
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, P. R. China.
authors.forename: Guy
authors.initials: G
authors.lastname: Smagghe
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Dou
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, P. R. China.
authors.forename: Jin-Jun
authors.initials: JJ
authors.lastname: Wang
authors.affiliation: Department of Crop Protection, Ghent University, Ghent, Belgium.
authors.forename: Guy
authors.initials: G
authors.lastname: Smagghe
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007313:Insecta / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D007306:Insecticides / Q000494:pharmacology; D007814:Larva / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D008294:Malathion / Q000494:pharmacology; D008297:Male; D009570:Nitriles / Q000494:pharmacology; D010063:Ovum / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D010574:Pesticide Synergists / Q000494:pharmacology; D010802:Phylogeny; D010882:Piperonyl Butoxide / Q000494:pharmacology; D011722:Pyrethrins / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; D019476:Insect Proteins; D007306:Insecticides; D009570:Nitriles; D010574:Pesticide Synergists; D011722:Pyrethrins; D012333:RNA, Messenger; C017180:decamethrin; D003577:Cytochrome P-450 Enzyme System; D010882:Piperonyl Butoxide; D008294:Malathion
keywords: PBO; cytochrome P450; insecticide induction; psocids; transcriptional profiles