title: Conformational and functional variants of CD44-targeted protein nanoparticles bio-produced in bacteria.
abstract: Biofabrication is attracting interest as a means to produce nanostructured functional materials because of its operational versatility and full scalability. Materials based on proteins are especially appealing, as the structure and functionality of proteins can be adapted by genetic engineering. Furthermore, strategies and tools for protein production have been developed and refined steadily for more than 30 years. However, protein conformation and therefore activity might be sensitive to production conditions. Here, we have explored whether the downstream strategy influences the structure and biological activities, in vitro and in vivo, of a self-assembling, CD44-targeted protein-only nanoparticle produced in Escherichia coli. This has been performed through the comparative analysis of particles built from soluble protein species or protein versions obtained by in vitro protein extraction from inclusion bodies, through mild, non-denaturing procedures. These methods have been developed recently as a convenient alternative to the use of toxic chaotropic agents for protein resolubilization from protein aggregates. The results indicate that the resulting material shows substantial differences in its physicochemical properties and its biological performance at the systems level, and that its building blocks are sensitive to the particular protein source.
authors.affiliation: Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain. Department de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain. CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Bellaterra, 08193 Barcelona, Spain.
authors.forename: Mireia
authors.initials: M
authors.lastname: Pesarrodona
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Fernández
authors.forename: Laia
authors.initials: L
authors.lastname: Foradada
authors.forename: Alejandro
authors.initials: A
authors.lastname: Sánchez-Chardi
authors.forename: Oscar
authors.initials: O
authors.lastname: Conchillo-Solé
authors.forename: Ugutz
authors.initials: U
authors.lastname: Unzueta
authors.forename: Zhikun
authors.initials: Z
authors.lastname: Xu
authors.forename: Mónica
authors.initials: M
authors.lastname: Roldán
authors.forename: Sandra
authors.initials: S
authors.lastname: Villegas
authors.forename: Neus
authors.initials: N
authors.lastname: Ferrer-Miralles
authors.forename: Simó
authors.initials: S
authors.lastname: Schwartz
authors.forename: Ursula
authors.initials: U
authors.lastname: Rinas
authors.forename: Xavier
authors.initials: X
authors.lastname: Daura
authors.forename: Ibane
authors.initials: I
authors.lastname: Abasolo
authors.forename: Esther
authors.initials: E
authors.lastname: Vázquez
authors.forename: Antonio
authors.initials: A
authors.lastname: Villaverde
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005818:Genetic Engineering; D018960:Hyaluronan Receptors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D002479:Inclusion Bodies / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D007218:Industrial Microbiology; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D012995:Solubility
chemical_list: C497483:CD44 protein, human; D018960:Hyaluronan Receptors