title: Signal Improvement Strategies for Fluorescence Detection of Biomacromolecules.
abstract: For analysis of biomacromolecules, a sensitive, specified and reliable method is indispensable. Fluorescent dyes or fluorophores have been widely used as mediums to obtain readout signals in various assays or bioimaging because of their versatilities such as biocompatibility. Those fluorescent dyes based techniques manipulate many molecular interactions for analysis of biomacromolecules including antibody-protein interaction, base complementation, glycan-lectin interaction, etc. The strategies to manipulate those molecular interactions are various and always updating due to the development of biotechnological tools and instruments. In this minireview, we summarize the state of the art of signal improvement techniques for fluorescence detection of biomacromolecules especially proteins and nucleic acids. We focus on the principle and mechanism of those techniques for fluorescence detection of biomacromolecules. We also discuss the future trend of the techniques for fluorescence detection of biomacromolecules.
authors.affiliation: Department of Hematology and Oncology (Key Department of Jiangsu Medicine), Zhongda Hospital, School of Medicine, Southeast University, Dingjiaqiao 87, Gulou District, Nanjing, 210009, Jiangsu Province, People's Republic of China.
authors.forename: Chengxin
authors.initials: C
authors.lastname: Luan
authors.affiliation: School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China.
authors.forename: Zixue
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Hematology and Oncology (Key Department of Jiangsu Medicine), Zhongda Hospital, School of Medicine, Southeast University, Dingjiaqiao 87, Gulou District, Nanjing, 210009, Jiangsu Province, People's Republic of China. cba8888@hotmail.com.
authors.forename: Baoan
authors.initials: B
authors.lastname: Chen
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D046911:Macromolecular Substances / Q000032:analysis* / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D046911:Macromolecular Substances
keywords: Biomacromolecule; Fluorescence; Signal improvement; Signal-to-noise ratio