title: Molecular characterization of Ambler class A to D β-lactamases, ISAba1, and integrons reveals multidrug-resistant Acinetobacter spp. isolates in northeastern China.
abstract: The prevalence of various Ambler class A to D β-lactamases, ISAba1, and class 1 and 2 integrons as well as the clonal relatedness in 105 Acinetobacter spp. isolates found in northeastern China was investigated. All 105 Acinetobacter spp. isolates were determined to be multidrug resistant (MDR), and the resistance rates to carbapenem agents were approximately 50%. PER, IMP, AmpC, and OXA-23 were found to be dominant β-lactamases belonging to different classes, respectively. This is the first report of the coexistence of blaPER, blaIMP, blaAmpC, and blaOXA-23-likegenes in Acinetobacter spp. isolates from northeastern China. ISAba1 was found upstream of the blaOXA-23-likegene in 87.8% (36/41) strains and upstream of the blaOXA-51-likegene in 26.5% (13/49) strains. ISAba3-like element was found upstream of the blaOXA-58-likegene in one blaOXA-58-like-positive strain. The presence of IntI1 was detected in 63.8% (67/105) of the isolates and the most prevalent gene cassettes were aacA4, aadA1, and catB8. The highly prevalent isolates belong to international clonal lineage (ICL)-II. These results indicate that the wide horizontal and clonal spread of MDR Acinetobacter spp. isolates harbouring multiple β-lactamase genes has become a serious problem in northeastern China.
authors.affiliation: a Key Laboratory of Zoonosis, Ministry of Education, Department of Pathogenobiology , College of Basic Medical Science, Jilin University , Changchun , P.R. China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Sun
authors.affiliation: b Cardiovascular Disease Center, The First Hospital of Jilin University , Changchun , P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: c Department of Neurosurgery , The Second Hospital of Jilin University , Changchun , P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: a Key Laboratory of Zoonosis, Ministry of Education, Department of Pathogenobiology , College of Basic Medical Science, Jilin University , Changchun , P.R. China.
authors.forename: Honglan
authors.initials: H
authors.lastname: Huang
authors.affiliation: a Key Laboratory of Zoonosis, Ministry of Education, Department of Pathogenobiology , College of Basic Medical Science, Jilin University , Changchun , P.R. China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Wang
authors.affiliation: a Key Laboratory of Zoonosis, Ministry of Education, Department of Pathogenobiology , College of Basic Medical Science, Jilin University , Changchun , P.R. China.
authors.forename: Fan
authors.initials: F
authors.lastname: Li
authors.affiliation: a Key Laboratory of Zoonosis, Ministry of Education, Department of Pathogenobiology , College of Basic Medical Science, Jilin University , Changchun , P.R. China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Ni
mesh_terms: D000150:Acinetobacter / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D015780:Carbapenems / Q000494:pharmacology; D002681:China; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D006801:Humans; D032023:Integrons; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D001618:beta-Lactamases
keywords: Acinetobacter spp.; ISAba1; Integron; Northeastern China; β-lactamases