title: Nitrogen and Sulfur Codoped Reduced Graphene Oxide as a General Platform for Rapid and Sensitive Fluorescent Detection of Biological Species.
abstract: Nitrogen (N) and sulfur (S) codoped reduced graphene oxide (N,S-rGO) was synthesized through a facile solvothermal process. The introduction of N and S heteroatoms into GO effectively activated the sp(2)-hybridized carbon lattice and made the material an ideal electron/energy acceptor. Such unique properties enable this material to perform as a general platform for rapid and sensitive detection of various biological species through simple fluorescence quenching and recovering. When quantum dot (QD)-labeled HBV (human being disease-related gene hepatitis B virus DNA) and HIV (human being disease-related gene human immunodeficiency virus DNA) molecular beacon probes were mixed with N,S-rGO, QD fluorescence was quenched; when target HBV and HIV DNA were added, QD fluorescence was recovered. By the recovered fluorescence intensity, the target virus DNA detection limits were reduced to 2.4 nM for HBV and 3.0 nM for HIV with detection time of less than 5 min. It must be stressed out that different viruses in the same homogeneous aqueous media could be discriminated and quantified simultaneously through choosing diverse QD probes with different colors. Moreover, even one mismatched target DNA could be distinguished using this method. When altering the molecular beacon loop domain to protein aptamers, this sensing strategy was also able to detect thrombin and IgE in 5 min with detection limits of 0.17 ng mL(-1) and 0.19 ng mL(-1), respectively, which was far more rapid and sensitive than bare GO-based fluorescence detection strategy.
authors.affiliation: College of Science, Huazhong Agricultural University , Wuhan 430070, People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
authors.affiliation: College of Science, Huazhong Agricultural University , Wuhan 430070, People's Republic of China.
authors.forename: Liping
authors.initials: L
authors.lastname: Song
authors.affiliation: College of Science, Huazhong Agricultural University , Wuhan 430070, People's Republic of China.|Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, People's Republic of China.
authors.forename: Yichi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Science, Huazhong Agricultural University , Wuhan 430070, People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: College of Science, Huazhong Agricultural University , Wuhan 430070, People's Republic of China.
authors.forename: Zhidong
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, People's Republic of China.
authors.forename: Yuguo
authors.initials: Y
authors.lastname: Guo
authors.affiliation: College of Science, Huazhong Agricultural University , Wuhan 430070, People's Republic of China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Cao
mesh_terms: D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes; D006108:Graphite; D006801:Humans; D009584:Nitrogen; D010087:Oxides; D013455:Sulfur / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D010087:Oxides; D013455:Sulfur; D006108:Graphite; D009584:Nitrogen
keywords: biomolecule sensing; nitrogen and sulfur codoping; quantum dot; reduced graphene oxide; virus DNA detection