title: The Hypolipidemic Effect of Total Saponins from Kuding Tea in High-Fat Diet-Induced Hyperlipidemic Mice and Its Composition Characterized by UPLC-QTOF-MS/MS.
abstract: Kuding tea are used as a traditional tea material and widely consumed in China. In this study, total saponins (TS) from water extract of Kuding tea was prepared by D101 macroporous resins and analyzed by UPLC-QTOF-MS/MS. Then the hypolipidemic effect of TS extract was investigated in high-fat diet-induced hyperlipidemic mice. For comprehensive identification or characterization of saponins in TS extract, 3 major saponins of Kudinoside A, Kudinoside F, and Kudinoside D were isolated and used as standards to investigate the MS/MS fragmentation pattern. As a result, 52 saponins were identified or characterized in TS extract from Kuding tea. In addition, the increased levels of mice serum TC, LDL-C, HDL-C, and atherogenic index (AI) were significantly reduced after the treatment of TS extract. Also, the liver protective effect of TS extract was obviously judged from the photographs stained with oil red-O staining. Meanwhile, TS extract significantly upregulated the expression of hepatic scavenger receptors including SR-AI, SR-BI, and CD36. Therefore, it is reasonable to assume that the overexpression of hepatic scavenger receptors was involved in the hypolipidemic effect of Kuding tea on the high-fat diet-induced hyperlipidemic mice. The TS extract could influence these scavenger receptors, and this could be the potential mechanism of TS extract from Kuding tea in the treatment of lipid disorders. These results give the evidence that the saponins in Kuding tea could provide benefits in managing hypercholesterolemia and may be a good candidate for development as a functional food and nutraceutical.
authors.affiliation: Tongji School of Pharmacy, Huazhong Univ. of Science and Technology, 13 Hangkong Road, Wuhan, Hubei, China.
authors.forename: Chengwu
authors.initials: C
authors.lastname: Song
authors.affiliation: Dept. of Otolaryngology, Union Hospital, Tongji Medical College, Huazhong Univ. of Science and Technology, 13 Hangkong Road, Wuhan, Hubei, China.
authors.forename: Qingsong
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Tongji School of Pharmacy, Huazhong Univ. of Science and Technology, 13 Hangkong Road, Wuhan, Hubei, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Environmental Health, School of Public Health, Tongji Medical College, Huazhong Univ. of Science and Technology, 13 Hangkong Road, Wuhan, Hubei, China.
authors.forename: Shuna
authors.initials: S
authors.lastname: Jin
authors.affiliation: Tongji School of Pharmacy, Huazhong Univ. of Science and Technology, 13 Hangkong Road, Wuhan, Hubei, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Li
authors.affiliation: Dept. of Pharmacy, Tongji Hospital of Tongji Medical College, Huazhong Univ. of Science and Technology, 13 Hangkong Road, Wuhan, Hubei, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Tongji School of Pharmacy, Huazhong Univ. of Science and Technology, 13 Hangkong Road, Wuhan, Hubei, China.
authors.forename: Shuailong
authors.initials: S
authors.lastname: Jia
authors.affiliation: College of Pharmacy, Hubei Univ. of Chinese Medicine, 1 Huangjiahu Road, Wuhan, Hubei, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Chen
authors.affiliation: Tongji School of Pharmacy, Huazhong Univ. of Science and Technology, 13 Hangkong Road, Wuhan, Hubei, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xiang
authors.affiliation: Tongji School of Pharmacy, Huazhong Univ. of Science and Technology, 13 Hangkong Road, Wuhan, Hubei, China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000818:Animals; D050197:Atherosclerosis; D028241:Camellia sinensis / Q000737:chemistry*; D002681:China; D002784:Cholesterol / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D059305:Diet, High-Fat; D006937:Hypercholesterolemia / Q000097:blood / Q000188:drug therapy / Q000209:etiology; D006949:Hyperlipidemias / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism; D000960:Hypolipidemic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D008055:Lipids / Q000097:blood*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D051116:Receptors, Scavenger / Q000378:metabolism; D012503:Saponins / Q000032:analysis / Q000494:pharmacology* / Q000627:therapeutic use; D053719:Tandem Mass Spectrometry / Q000379:methods; D013662:Tea / Q000737:chemistry*; D014315:Triterpenes / Q000032:analysis / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000960:Hypolipidemic Agents; D008055:Lipids; D010936:Plant Extracts; D051116:Receptors, Scavenger; D012503:Saponins; D013662:Tea; D014315:Triterpenes; C102126:kudinoside A; C580614:kudinoside D; C580612:kudinoside F; D002784:Cholesterol
keywords: Kuding tea; UPLC-QTOF-MS/MS; hyperlipidemia; scavenger receptors; total saponins