title: Turmeric effect on subcutaneous insulin-induced amyloid mass: an in vivo study.
abstract: Protein-derived amyloid structures are associated with a wide variety of pathologies, including neurodegenerative diseases and local amyloidoses. Reports exist on the ability of insulin to form local amyloidoses under specific conditions. In vitro-generated fibrils of insulin have been previously shown to produce amyloid-containing masses upon repetitive subcutaneous injection in mouse. The present study aimed at investigating the effect of insulin fibrils injection in rats, as well as the potential of turmeric in attenuating this process. It was found that subcutaneous amyloid-containing masses could form in rats at a faster rate compared with mice. Upon addition of turmeric to the fibrils, previous to injection, formed masses had a significantly reduced size, as well as less ordered cellular structure. In conclusion, the results of this study show the potential of turmeric in attenuation of local amyloidosis. Furthermore, we suggest that this model could be of use in screening antiamyloid compounds.
authors.affiliation: a Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences , Tehran , Iran.|b Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Raheleh
authors.initials: R
authors.lastname: Kheirbakhsh
authors.affiliation: b Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Chinisaz
authors.affiliation: c Cancer Models Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Saeid
authors.initials: S
authors.lastname: Amanpour
authors.affiliation: b Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Shahab
authors.initials: S
authors.lastname: Amini
authors.affiliation: d Islamic Azad University, Tehran Medical Branch , Tehran , Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Khodayari
authors.affiliation: e Pharmaceutical Sciences Branch and Pharmaceutical Sciences Research Center, Islamic Azad University , Tehran , Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Khodayari
authors.affiliation: f Department of Basic Sciences , Division of Anatomical Sciences, Faculty of Veterinary Medicine, University of Tehran , Tehran , Iran , and.
authors.forename: Aydin
authors.initials: A
authors.lastname: Dilmaghanian
authors.affiliation: g Vali E Asr Reproductive Health Research Center, Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Haddadi
authors.affiliation: b Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Azadeh
authors.initials: A
authors.lastname: Ebrahim-Habibi
mesh_terms: D000682:Amyloid / Q000378:metabolism*; D000686:Amyloidosis / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D030024:Curcuma / Q000737:chemistry*; D003474:Curcumin / Q000008:administration & dosage / Q000494:pharmacology*; D007279:Injections, Subcutaneous; D007328:Insulin / Q000008:administration & dosage / Q000009:adverse effects*; D008297:Male; D008856:Microscopy, Fluorescence; D008859:Microscopy, Polarization; D017208:Rats, Wistar
chemical_list: D000682:Amyloid; D007328:Insulin; D003474:Curcumin
keywords: Amyloid model; Congo red; Thioflavin S; Wistar rat