title: Novel stress increases hypothalamic-pituitary-adrenal activity in mice with a raised bite.
abstract: OBJECTIVE
  In humans, occlusal disharmony may cause various physical complaints, including head and neck ache, stiffness in the shoulder and neck, and arthrosis of the temporomandibular joints. Occlusal disharmony induced by raising the bite in rodents, increases plasma corticosterone levels, which leads to morphologic changes in the hippocampus and altered hippocampus-related behavior. The paraventricular nucleus (PVN) of the hypothalamus regulates the hypothalamic-pituitary-adrenal system. Chronically stressed animals exposed to a novel stress exhibit higher adrenocorticotropic hormone levels than naive control animals. We hypothesized that there would be different response of the corticotrophin releasing hormone (CRH) and arginine vasopressin (AVP) to a novel acute stress with occlusal disharmony.
  
  
  METHODS
  In order to investigate how exposure of mice with occlusal disharmony to a novel acute stress (restraint stress) affects the PVN, we induced occlusal disharmony by raising the vertical dimension of the bite (bite-raised condition) and examined the expression of corticotrophin releasing hormone (CRH) mRNA and arginine vasopressin (AVP) mRNA in mouse PVN.
  
  
  RESULTS
  CRH mRNA expression was increased in the PVN of the bite-raised group 90min after the bite-raising procedure, but the expression was recovered to the control level at 14days. AVP mRNA expression in the PVN was normal at 90min, and increased significantly 14days after the bite-raising procedure. Exposure to restraint stress in the bite-raised mice induced a significant increase in CRH mRNA expression in the PVN.
  
  
  CONCLUSIONS
  The bite-raising procedure induced a rapid CRH mRNA response and a slower AVP mRNA response in the parvocellular PVN of the hypothalamus. Exposure to a novel stress following the bite-raising procedure further reinforced the CRH stress response. Thus, occlusal disharmony, such as that induced by raising the bite, may be a risk factor for hypersensitivity to a novel stress.
authors.affiliation: Department of Prosthodontics, Asahi University School of Dentistry, 1851 Hozumi, Mizuho, Gifu, 501-0296, Japan.
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Miyake
authors.affiliation: Department of Prosthodontics, Asahi University School of Dentistry, 1851 Hozumi, Mizuho, Gifu, 501-0296, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Mori
authors.affiliation: Department of Prosthodontics, Asahi University School of Dentistry, 1851 Hozumi, Mizuho, Gifu, 501-0296, Japan.
authors.forename: Tasuku
authors.initials: T
authors.lastname: Katayama
authors.affiliation: Department of Prosthodontics, Asahi University School of Dentistry, 1851 Hozumi, Mizuho, Gifu, 501-0296, Japan.
authors.forename: Shuu
authors.initials: S
authors.lastname: Fujiwara
authors.affiliation: Department of Molecular Diagnostics, Kitasato University School of Allied Health Science, Kitasato 1-15-1, Minamiku, Sagamihara, Kanagawa, 252-0373, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Sato
authors.affiliation: Department of Anatomy, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, Fukuoka, 807-8555, Japan.
authors.forename: Kagaku
authors.initials: K
authors.lastname: Azuma
authors.affiliation: Seijoh University Graduate School of Health Care Studies, 2-172, Fukinodai, Tokai, Aichi, 476-8588, Japan. Electronic address: kubo@seijoh-u.ac.jp.
authors.forename: Kin-Ya
authors.initials: KY
authors.lastname: Kubo
mesh_terms: D000818:Animals; D001127:Arginine Vasopressin / Q000096:biosynthesis / Q000378:metabolism; D001732:Bite Force; D003345:Corticosterone / Q000097:blood / Q000494:pharmacology; D003346:Corticotropin-Releasing Hormone / Q000096:biosynthesis / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism*; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism; D010913:Pituitary-Adrenal System / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D013312:Stress, Physiological / Q000502:physiology*
chemical_list: D012333:RNA, Messenger; D001127:Arginine Vasopressin; D003346:Corticotropin-Releasing Hormone; D003345:Corticosterone
keywords: Corticotrophin releasing hormone; HPA-axis; Novel stress; Occlusal disharmony; Vasopressin