title: Isolation, identification and diesel-oil biodegradation capacities of indigenous hydrocarbon-degrading strains of Cellulosimicrobium cellulans and Acinetobacter baumannii from tarball at Terengganu beach, Malaysia.
abstract: In this study, we isolated two indigenous hydrocarbon-degrading bacteria from tarball found in Rhu Sepuluh beach, Terengganu, Malaysia. These bacteria were identified based on their physiological characteristic and 16S rRNA gene sequence analysis, and they showed 99% similarity with Cellulosimicrobium cellulans DSM 43879 and Acinetobacter baumannii ATCC 19606 respectively. Their hydrocarbon-degrading capabilities were tested using diesel-oil as sole carbon source. Results analysed using GC-MS, showed diesel-oil alkanes were degraded an average 64.4% by C. cellulans and 58.1% by A. baumannii with medium optical density reaching 0.967 (C. cellulans) and 1.515 (A. baumannii) in minimal salt media at 32°C for 10days. Individual diesel-oil alkanes were degraded between 10%-95.4% by C. cellulans and 0.2%-95.9% by A. baumannii. Both strains utilized diesel-oil for growth. The study suggests both strains are part of indigenous hydrocarbon-degrading bacteria in tarball with potential for bioremediation of oil-polluted marine environment.
authors.affiliation: Faculty of Environmental Studies, Department of Environmental Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia.
authors.forename: Bruno Martins
authors.initials: BM
authors.lastname: Nkem
authors.affiliation: Faculty of Environmental Studies, Department of Environmental Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia. Electronic address: mala_upm@upm.edu.my.
authors.forename: Normala
authors.initials: N
authors.lastname: Halimoon
authors.affiliation: Laboratory of Marine Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia.
authors.forename: Fatimah Md
authors.initials: FM
authors.lastname: Yusoff
authors.affiliation: Faculty of Environmental Studies, Department of Environmental Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia.
authors.forename: Wan Lufti Wan
authors.initials: WLW
authors.lastname: Johari
authors.affiliation: Faculty of Environmental Studies, Department of Environmental Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia.
authors.forename: Mohamad Pauzi
authors.initials: MP
authors.lastname: Zakaria
authors.affiliation: Laboratory of Marine Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia.
authors.forename: Srikanth Reddy
authors.initials: SR
authors.lastname: Medipally
authors.affiliation: Faculty of Environmental Studies, Department of Environmental Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia.
authors.forename: Narayanan
authors.initials: N
authors.lastname: Kannan
mesh_terms: D040981:Acinetobacter baumannii / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004783:Environmental Microbiology; D006838:Hydrocarbons / Q000378:metabolism*; D008296:Malaysia; D010578:Petroleum / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D006838:Hydrocarbons; D010578:Petroleum; D012336:RNA, Ribosomal, 16S
keywords: Acinetobacter baumannii; Bioremediation; Cellulosimicrobium cellulans; Diesel-oil; Hydrocarbons; Tarball