title: High cholesterol diet leads to oxidant load and peroxidation in the rabbit kidney tissue.
abstract: OBJECTIVE
  The aim of this study was to investigate possible effects of high cholesterol diet on oxidant/antioxidant status in rabbit kidney tissues.
  
  
  BACKGROUND
  Although a number of experimental animal models have suggested that hyperlipidemia is associated with progressive kidney failure data remain sparse on the role of dietary cholesterol intake on kidney disease.
  
  
  METHODS
  Twelve male New Zealand albino rabbits were randomly divided into two groups (control and cholesterol). Both groups were fed on a standard laboratory diet. Animals in the cholesterol group additionally received cholesterol (1 g/kg/day), orally. The study period was 12 weeks. Activities of catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), nitric oxide synthase (NOS), xanthine oxidase (XO), paraoxonase (PON), adenosine deaminase (ADA) enzymes and levels of malondialdehyde (MDA) and nitric oxide (NO) were measured in kidney tissue samples. Histological examination of the kidney tissue samples was also done.
  
  
  RESULTS
  SOD, GSH-Px and XO enzyme activities were found to be decreased and NOS and PON activities increased significantly in cholesterol group compared to controls. As an indication of oxidation, MDA levels were found to be increased in cholesterol group. Histological examination revealed some derangements in the kidney tissue.
  
  
  CONCLUSIONS
  High cholesterol diet creates oxidant load and causes peroxidation, which in turn, leads derangements in the rabbit kidney tissue (Tab. 2, Fig. 2, Ref. 69).
authors.forename: B
authors.initials: B
authors.lastname: Ozturk
authors.forename: O
authors.initials: O
authors.lastname: Ozer
authors.forename: Z E
authors.initials: ZE
authors.lastname: Durak
authors.forename: D
authors.initials: D
authors.lastname: Billur
authors.forename: S
authors.initials: S
authors.lastname: Kizil
authors.forename: I
authors.initials: I
authors.lastname: Durak
authors.forename: H S
authors.initials: HS
authors.lastname: Ozturk
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002791:Cholesterol, Dietary / Q000008:administration & dosage / Q000378:metabolism; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D005979:Glutathione Peroxidase / Q000378:metabolism; D006949:Hyperlipidemias / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase; D016877:Oxidants / Q000378:metabolism; D011817:Rabbits; D013482:Superoxide Dismutase / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002791:Cholesterol, Dietary; D016877:Oxidants; D009569:Nitric Oxide; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D019001:Nitric Oxide Synthase; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase
keywords: cholesterol; kidney; oxidant / antioxidant system; rabbit.