title: Sub-MIC Tylosin Inhibits Streptococcus suis Biofilm Formation and Results in Differential Protein Expression.
abstract: Streptococcus suis (S.suis) is an important zoonotic pathogen that causes severe diseases in humans and pigs. Biofilms of S. suis can induce persistent infections that are difficult to treat. In this study, the effect of tylosin on biofilm formation of S. suis was investigated. 1/2 minimal inhibitory concentration (MIC) and 1/4 MIC of tylosin were shown to inhibit S. suis biofilm formation in vitro. By using the iTRAQ strategy, we compared the protein expression profiles of S. suis grown with sub-MIC tylosin treatment and with no treatment. A total of 1501 proteins were identified by iTRAQ. Ninety-six differentially expressed proteins were identified (Ratio > ±1.5, p < 0.05). Several metabolism proteins (such as phosphoglycerate kinase) and surface proteins (such as ABC transporter proteins) were found to be involved in biofilm formation. Our results indicated that S. suis metabolic regulation, cell surface proteins, and virulence proteins appear to be of importance in biofilm growth with sub-MIC tylosin treatment. Thus, our data revealed the rough regulation of biofilm formation that may provide a foundation for future research into mechanisms and targets.
authors.affiliation: Department of Veterinary Pharmacy, College of Veterinary Medicine, Northeast Agricultural University Harbin, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Veterinary Pharmacy, College of Veterinary Medicine, Northeast Agricultural University Harbin, China.
authors.forename: Yanbei
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Veterinary Pharmacy, College of Veterinary Medicine, Northeast Agricultural University Harbin, China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Biotechnology, Heilongjiang Vocational College for Nationalities Harbin, China.
authors.forename: Honghai
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Veterinary Pharmacy, College of Veterinary Medicine, Northeast Agricultural University Harbin, China.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Bai
authors.affiliation: Department of Veterinary Pharmacy, College of Veterinary Medicine, Northeast Agricultural University Harbin, China.
authors.forename: Jianqing
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Veterinary Pharmacy, College of Veterinary Medicine, Northeast Agricultural University Harbin, China.
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Veterinary Pharmacy, College of Veterinary Medicine, Northeast Agricultural University Harbin, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Veterinary Pharmacy, College of Veterinary Medicine, Northeast Agricultural University Harbin, China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Li
keywords: S. suis; biofilms; iTRAQ; proteomics; tylosin