title: MiR-142-5p promotes bone repair by maintaining osteoblast activity.
abstract: MicroRNAs play important roles in regulating bone regeneration and remodeling. However, the pathophysiological roles of microRNAs in bone repair remain unclear. Here we identify a significant upregulation of miR-142-5p correlated with active osteoblastogenesis during the bone healing process. In vitro, miR-142-5p promoted osteoblast activity and matrix mineralization by targeting the gene encoding WW-domain-containing E3 ubiquitin protein ligase 1. We also found that the expression of miR-142-5p in the callus of aged mice was lower than that in the callus of young mice and directly correlated with the age-related delay in bone healing. Furthermore, treatment with agomir-142-5p in the fracture areas stimulated osteoblast activity which repaired the bone fractures in aged mice. Thus, our study revealed that miR-142-5p plays a crucial role in healing fractures by maintaining osteoblast activity, and provided a new molecular target therapeutic strategy for bone healing.
authors.affiliation: Institute of Endocrinology and Metabolism, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, People's Republic of China.
authors.forename: Manli
authors.initials: M
authors.lastname: Tu
authors.affiliation: Department of Gynaecology and Obstetrics, The First Hospital Affiliated to Nanjing Medical University, Nanjing, 210029, Jiangsu, People's Republic of China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Tang
authors.affiliation: Department of Orthopedics, Xiangya Hospital of Central South University, 87# Xiangya Road, Changsha, 410008, Hunan, People's Republic of China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: He
authors.affiliation: Department of Gerontology, The First Hospital Affiliated to Nanjing Medical University, Nanjing, 210029, Jiangsu, People's Republic of China. cphh@sohu.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Cheng
authors.affiliation: Department of Endocrinology and Metabolism, The First Affiliated Hospital of Soochow University, Suzhou, 215006, Jiangsu, People's Republic of China. chaochen2005@163.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
mesh_terms: D000818:Animals; D000070416:Antagomirs / Q000378:metabolism; D001483:Base Sequence; D001857:Bone Matrix / Q000378:metabolism; D001842:Bone and Bones / Q000378:metabolism / Q000473:pathology*; D002146:Bony Callus / Q000473:pathology; D002113:Calcification, Physiologic / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D010006:Osteoblasts / Q000378:metabolism* / Q000473:pathology*; D010012:Osteogenesis / Q000235:genetics; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism; D014945:Wound Healing / Q000235:genetics*
chemical_list: D000070416:Antagomirs; D035683:MicroRNAs; C529102:Mirn142 microRNA, mouse; C510836:WWP1 protein, mouse; D044767:Ubiquitin-Protein Ligases
keywords: Bone fracture; MicroRNA; Osteoblastogenesis; Wwp1