title: Genetic diversity in Ebola virus: Phylogenetic and in silico structural studies of Ebola viral proteins.
abstract: OBJECTIVE
  To explore the genetic diversity and the modification of antibody response in the recent outbreak of Ebola Virus.
  
  
  METHODS
  Sequences retrieved from public databases, the selective pressure analysis and the homology modeling based on the all protein (nucleoprotein, VP35, VP40, soluble glycoprotein, small soluble glycoprotein, VP30, VP24 and polymerase) were used.
  
  
  RESULTS
  Structural proteins VP24, VP30, VP35 and VP40 showed relative conserved sequences making them suitable target candidates for antiviral treatment. On the contrary, nucleoprotein, polymerase and soluble glycoprotein have high mutation frequency.
  
  
  CONCLUSIONS
  Data from this study point out important aspects of Ebola virus sequence variability that for epitope and vaccine design should be considered for appropriate targeting of conserved protein regions.
authors.affiliation: ProxAgen Ltd, Sofia, Bulgaria.
authors.forename: Alba
authors.initials: A
authors.lastname: Grifoni
authors.affiliation: Department of Infectious Parasitic and Immunomediated Diseases, National Institute of Health, Rome, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Lo Presti
authors.affiliation: Department of Infectious Parasitic and Immunomediated Diseases, National Institute of Health, Rome, Italy; Department of Biology, University of Rome 'Tor Vergata', Rome, Italy.
authors.forename: Marta
authors.initials: M
authors.lastname: Giovanetti
authors.affiliation: Department of Biology, University of Rome 'Tor Vergata', Rome, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Montesano
authors.affiliation: ProxAgen Ltd, Sofia, Bulgaria; Department of Biomedicine and Prevention, University of Rome 'Tor Vergata', Rome, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Amicosante
authors.affiliation: Department of Biology, University of Rome 'Tor Vergata', Rome, Italy.
authors.forename: Vittorio
authors.initials: V
authors.lastname: Colizzi
authors.affiliation: Laboratory of Infectious Diseases and Tropical Medicine, University of Milan, Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Lai
authors.affiliation: Laboratory of Infectious Diseases and Tropical Medicine, University of Milan, Italy.
authors.forename: Gianguglielmo
authors.initials: G
authors.lastname: Zehender
authors.affiliation: Department of Infectious Parasitic and Immunomediated Diseases, National Institute of Health, Rome, Italy; Department of Public Health and Infectious Diseases, Sapienza University of Rome, Rome, Italy.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Cella
authors.affiliation: Clinical Pathology and Microbiology Laboratory, University Hospital Campus Bio-Medico of Rome, Rome, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Angeletti
authors.affiliation: Department of Infectious Parasitic and Immunomediated Diseases, National Institute of Health, Rome, Italy; University Campus Bio-Medico, Rome, Italy. Electronic address: ciccozzi@iss.it.
authors.forename: Massimo
authors.initials: M
authors.lastname: Ciccozzi
keywords: Ebola virus; Evolutionary analysis; Proteins