title: Jasmonate-Responsive ERF Transcription Factors Regulate Steroidal Glycoalkaloid Biosynthesis in Tomato.
abstract: Steroidal glycoalkaloids (SGAs) are cholesterol-derived specialized metabolites produced in species of the Solanaceae. Here, we report that a group of jasmonate-responsive transcription factors of the ETHYLENE RESPONSE FACTOR (ERF) family (JREs) are close homologs of alkaloid regulators in Cathranthus roseus and tobacco, and regulate production of SGAs in tomato. In transgenic tomato, overexpression and dominant suppression of JRE genes caused drastic changes in SGA accumulation and in the expression of genes for metabolic enzymes involved in the multistep pathway leading to SGA biosynthesis, including the upstream mevalonate pathway. Transactivation and DNA-protein binding assays demonstrate that JRE4 activates the transcription of SGA biosynthetic genes by binding to GCC box-like elements in their promoters. These JRE-binding elements occur at significantly higher frequencies in proximal promoter regions of the genes regulated by JRE genes, supporting the conclusion that JREs mediate transcriptional co-ordination of a series of metabolic genes involved in SGA biosynthesis.
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, 630-0101 Japan.
authors.forename: Chonprakun
authors.initials: C
authors.lastname: Thagun
authors.affiliation: Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization, Tsu, 514-2392 Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Imanishi
authors.affiliation: Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, 214-8571 Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Kudo
authors.affiliation: RIKEN Center for Sustainable Resource Science, Yokohama, 230-0045 Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Nakabayashi
authors.affiliation: Department of Chemistry and Materials Science, Tokyo Institute of Technology, Meguro-ku, 152-8551 Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ohyama
authors.affiliation: RIKEN Center for Sustainable Resource Science, Yokohama, 230-0045 Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mori
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, 305-8577 Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Kawamoto
authors.affiliation: Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, 214-8571 Japan.
authors.forename: Yukino
authors.initials: Y
authors.lastname: Nakamura
authors.affiliation: Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, 214-8571 Japan.
authors.forename: Minami
authors.initials: M
authors.lastname: Katayama
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, 305-8577 Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Nonaka
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, 305-8577 Japan.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Matsukura
authors.affiliation: Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, 214-8571 Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yano
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, 305-8577 Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ezura
authors.affiliation: RIKEN Center for Sustainable Resource Science, Yokohama, 230-0045 Japan Graduate School of Pharmaceutical Sciences, Chiba University, Chiba, 260-8675 Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Saito
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, 630-0101 Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Hashimoto
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, 630-0101 Japan t-shouji@bs.naist.jp.
authors.forename: Tsubasa
authors.initials: T
authors.lastname: Shoji
mesh_terms: D000470:Alkaloids / Q000096:biosynthesis; D003517:Cyclopentanes / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005030:Ethylenes / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D018551:Solanum lycopersicum / Q000235:genetics* / Q000502:physiology; D054883:Oxylipins / Q000378:metabolism*; D010840:Phytosterols / Q000096:biosynthesis*; D010937:Plant Growth Regulators / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D013045:Species Specificity; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D000470:Alkaloids; D003517:Cyclopentanes; D004268:DNA-Binding Proteins; D005030:Ethylenes; D054883:Oxylipins; D010840:Phytosterols; D010937:Plant Growth Regulators; D010940:Plant Proteins; D014157:Transcription Factors; C011006:jasmonic acid; C036216:ethylene
keywords: Jasmonates; Steroidal glycoalkaloids; Tomato; Transcription factors