title: Seed germination of medicinal plant, fennel (Foeniculum vulgare Mill), as affected by different priming techniques.
abstract: Reduced seed germination is among the most important factors adversely affecting crop stand and subsequent plant growth. Fennel (Foeniculum vulgare Mill) is an important medicinal plant with poor seed germination rate, occasionally. It is accordingly pertinent to find methods which can enhance fennel seed germination and remove the barriers of dormancy breaking. The present experiments studied the effects of two different priming (cold moist stratification and osmopriming) and 14 dormancy breaking techniques (hormonal, osmopriming, biopriming, chemical priming, and hydropriming) on the seed germination and seedling growth of two different fennel genotypes under growth chamber conditions. In the first and second experiment, the priming techniques including the time lengths of cold moist stratification (0, 15, 30, and 45 days) and the concentrations of polyethylene glycol 6000 (PEG6000, osmopriming at -0.99, -1.35, and -2.33 MPa) were used as the main plots. However, in both experiments, the dormancy breaking techniques and fennel genotypes were factorially combined and used as the subplots. Different seed- and seedling-related parameters including germination (%), plumule, radicle and seedling length, average germination time, rate and homogeneity of germination, and seed vigor index were determined. Both priming techniques were efficient on the enhancement of seed germination and seedling growth. Among the dormancy breaking techniques, Aminol Forte (biopriming), kadostim (biopriming), benzyl adenine + kinetin (biopriming), distilled water (hydropriming), gibberellin + kinetin (hormonal priming), and benzyl adenine + kinetin + gibberellin (biopriming) were the most effective ones. The related concentrations were equal to 100 mg/l, 10(-5) M, and 0.4 %. The fennel genotypes reacted significantly different under priming conditions. It is possible to enhance seed germination and seedling growth of fennel using priming and dormancy breaking techniques, which is useful for the increased production of fennel under different conditions. The results indicate that bio and hydropriming techniques were among the most effective ones, which significantly increased seed germination and seedling growth, and removed the seed dormancy barriers.
authors.affiliation: Department of Agronomy and Plant Breeding, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.
authors.forename: Amirreza
authors.initials: A
authors.lastname: Tahaei
authors.affiliation: Department of Agronomy and Plant Breeding, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran. A_soleymani@khuisf.ac.ir.
authors.forename: Ali
authors.initials: A
authors.lastname: Soleymani
authors.affiliation: Department of Plant Breeding, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Shams
mesh_terms: D000704:Analysis of Variance; D030001:Foeniculum / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D005838:Genotype; D018525:Germination / Q000187:drug effects; D057445:Plant Dormancy / Q000187:drug effects; D010946:Plants, Medicinal / Q000187:drug effects / Q000254:growth & development*; D011092:Polyethylene Glycols / Q000494:pharmacology; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development; D012639:Seeds / Q000187:drug effects / Q000254:growth & development*
chemical_list: D011092:Polyethylene Glycols
keywords: Fennel (Foeniculum vulgare Mill); Priming techniques; Seed germination; Seedling growth