title: [Expression of Rictor and mTOR in colorectal cancer and their clinical significance].
abstract: OBJECTIVE
  To explore the expression of Rictor and mTOR in the colorectal cancer and their clinical significance.
  
  
  METHODS
  The expression levels of Rictor and mTOR in HCT116, SW480, LoVo and HCoEpiC cells were detected by indirect immunofluorescence and Western blotting. Sixty-two paraffin-embedded surgical specimens of colorectal cancer tissue and adjacent tissues were examined for Rictor expression using immunohistochemistry. The association of the expression levels of Rictor protein with the clinicopathologic features and the overall survival of the patients was analyzed.
  
  
  RESULTS
  The expression level of Rictor was significantly higher in colorectal cancer tissues than in the adjacent tissues (P<0.05). The expression levels of Rictor and mTOR in the colon cancer cell lines were higher than those in human normal colon epithelial cell line HCoEpiC. The expression of Rictor was correlated with Dukes stage and lymphatic metastasis of the tumors but not with other clinicopathological parameter (P>0.05). Patients with Rictor expression had a lower overall survival rate than those without Rictor expression.
  
  
  CONCLUSIONS
  Rictor overexpression is associated with the carcinogenesis and progression of colorectal cancer and can be an independent indicator for evaluating the prognosis of colorectal cancer patients.
authors.affiliation: Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.E-mail: wanglifeng890103@aliyun.com.
authors.forename: Li-Feng
authors.initials: LF
authors.lastname: Wang
authors.forename: Hai-Jin
authors.initials: HJ
authors.lastname: Chen
authors.forename: Jin-Long
authors.initials: JL
authors.lastname: Yu
authors.forename: Jia
authors.initials: J
authors.lastname: Qi
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Lin
authors.forename: Zhao-Wei
authors.initials: ZW
authors.lastname: Zou
mesh_terms: D015153:Blotting, Western; D002352:Carrier Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000378:metabolism*; D018450:Disease Progression; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D011379:Prognosis; D000076226:Rapamycin-Insensitive Companion of mTOR Protein; D015996:Survival Rate; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; C492571:RICTOR protein, human; D000076226:Rapamycin-Insensitive Companion of mTOR Protein; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases