title: Apoptotic protein profile in Leishmania donovani after treatment with hexaazatrinaphthylenes derivatives.
abstract: Two hexaazatrinaphthylene derivatives, DGV-B and DGV-C previously known to induce an apoptotic-like process in Leishmania donovani parasites were used in this study. For this purpose, two different human protein commercial arrays were used to determine the proteomic profile of the treated parasites compared to non-treated ones. One of the commercial arrays is able to detect the relative expression of 35 human apoptosis-related proteins and the other one is able to identify 9 different human kinases. The obtained results showed that the two tested molecules were able to activate a programmed cell death process by different pathways in the promastigote stage of the parasite. The present study reports the potential application of two commercialised human apoptotic arrays to evaluate the action mechanism of active compounds at least against Leishmania donovani. The obtained data would be useful to establish the putative activated apoptosis pathways in the treated parasites and to further support the use of hexaazatrinaphthylene derivatives for the treatment of leishmaniasis in the near future. Nevertheless, further molecular studies should be developed in order to design and evaluate specific apoptotic arrays for Leishmania genus.
authors.affiliation: University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, La Laguna, Canary Islands, Spain. Electronic address: atlopez@ull.edu.es.
authors.forename: Atteneri
authors.initials: A
authors.lastname: López-Arencibia
authors.affiliation: University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, La Laguna, Canary Islands, Spain.
authors.forename: Carmen M
authors.initials: CM
authors.lastname: Martín-Navarro
authors.affiliation: Laboratoire Matériaux-Molécules et Applications, IPEST, University of Carthage, La Marsa, Tunisia.
authors.forename: Ines
authors.initials: I
authors.lastname: Sifaoui
authors.affiliation: University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, La Laguna, Canary Islands, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Reyes-Batlle
authors.affiliation: University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, La Laguna, Canary Islands, Spain; Cátedra de Parasitología, Escuela de Bioanálisis, Facultad de Medicina, Universidad Central de Venezuela, Caracas, Venezuela.
authors.forename: Carolina
authors.initials: C
authors.lastname: Wagner
authors.affiliation: University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, La Laguna, Canary Islands, Spain.
authors.forename: Jacob
authors.initials: J
authors.lastname: Lorenzo-Morales
authors.affiliation: University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, La Laguna, Canary Islands, Spain.
authors.forename: José E
authors.initials: JE
authors.lastname: Piñero
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007893:Leishmania donovani / Q000737:chemistry* / Q000187:drug effects; D007898:Leishmaniasis, Visceral / Q000188:drug therapy; D009281:Naphthalenes / Q000737:chemistry / Q000494:pharmacology*; D010766:Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism; D040081:Protein Array Analysis; D020543:Proteome; D015800:Protozoan Proteins / Q000187:drug effects* / Q000378:metabolism
chemical_list: D009281:Naphthalenes; D020543:Proteome; D015800:Protozoan Proteins; C547330:hexaazatrinaphthylene; D010770:Phosphotransferases
keywords: Apoptosis; Array; Hexaazatrinaphthylene; Leishmania; Proteins