title: Dopamine D1 receptor agonist A-68930 inhibits NLRP3 inflammasome activation and protects rats from spinal cord injury-induced acute lung injury.
abstract: METHODS
  Randomized experimental study.
  
  
  OBJECTIVE
  The study aimed to investigate the therapeutic efficacy and molecular mechanisms of A-68930 in a rat model of spinal cord injury (SCI)-induced acute lung injury (ALI).
  
  
  METHODS
  China.
  
  
  METHODS
  The influences of A-68930 on the pulmonary edema, histological changes, proinflammatory cytokines levels, myeloperoxidase (MPO) activity and NLRP3 inflammasome protein expression were estimated.
  
  
  RESULTS
  SCI significantly promoted NLRP3 inflammasome activation, increased proinflammatory cytokine productions and MPO activity, and induced pulmonary edema and tissue damage in the SCI group as compared with the control group. A-68930 administration significantly inhibited NLRP3 inflammasome activation and reduced inflammatory cytokines levels and MPO activity. Moreover, A-68930 administration attenuated pulmonary edema and histopathology.
  
  
  CONCLUSIONS
  Our experimental findings indicated that A-68930 exhibited a protective effect on SCI-induced ALI by the alleviations of inflammatory response with the inhibition NLRP3 inflammasome activation 72 h post injury. The present study indicated that A-68930 could be a potentially efficient therapeutic strategy for the treatment of SCI-induced ALI.
authors.affiliation: Department of Orthopedics, Nanjing Medical University, Affiliated Hangzhou Hospital (Hangzhou First People's Hospital), Hangzhou, China.
authors.forename: W
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Department of Orthopedics, Nanjing Medical University, Affiliated Hangzhou Hospital (Hangzhou First People's Hospital), Hangzhou, China.
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Nanjing Medical University, Affiliated Hangzhou Hospital (Hangzhou First People's Hospital), Hangzhou, China.
authors.forename: F
authors.initials: F
authors.lastname: He
authors.affiliation: Department of Orthopedics, Nanjing Medical University, Affiliated Hangzhou Hospital (Hangzhou First People's Hospital), Hangzhou, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Bian
authors.affiliation: Department of Orthopaedics, General Hospital of Beijing Military Command, Beijing, China.
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, Shanghai Jiao Tong University affiliated Sixth People's Hospital, Shanghai, China.
authors.forename: Q
authors.initials: Q
authors.lastname: He
authors.affiliation: Department of Orthopedics, Nanjing Medical University, Affiliated Hangzhou Hospital (Hangzhou First People's Hospital), Hangzhou, China.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, General Hospital of Beijing Military Command, Beijing, China.
authors.forename: T
authors.initials: T
authors.lastname: Sun
authors.affiliation: Department of Orthopedics, Nanjing Medical University, Affiliated Hangzhou Hospital (Hangzhou First People's Hospital), Hangzhou, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhu
mesh_terms: D055371:Acute Lung Injury / Q000188:drug therapy* / Q000209:etiology*; D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects; D002839:Chromans / Q000627:therapeutic use*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D018491:Dopamine Agonists / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein / Q000378:metabolism*; D009929:Organ Size / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000150:complications*; D013997:Time Factors
chemical_list: D002839:Chromans; D016207:Cytokines; D018491:Dopamine Agonists; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C573346:Nlrp3 protein, rat; C065380:A 68930; D009195:Peroxidase