title: Tanshinone IIA Induces Heme Oxygenase 1 Expression and Inhibits Cyclic Strain-Induced Interleukin 8 Expression in Vascular Endothelial Cells.
abstract: Tanshinone IIA is the main effective component of Salvia miltiorrhiza, known as "Danshen," which has been used in many therapeutic remedies in traditional Chinese medicine. However, the direct effects of tanshinone IIA on vascular endothelial cells have not yet been fully described. In the present study, we demonstrated that tanshinone IIA increased heme oxygenase-1 (HO-1) expression in human umbilical vein endothelial cells. Western blot analyses and experiments with specific inhibitors indicated tanshinone IIA enhanced HO-1 expression through the activation of phosphoinositide 3-kinase (PI3K)/Akt and the subsequent induction of nuclear factor erythroid 2-related factor 2 (Nrf2) nuclear translocation. In addition, tanshinone IIA inhibited cyclic strain induced interleukin-8 (IL-8) expression. HO-1 silencing significantly abrogated the repressive effects of tanshinone IIA on strain-induced IL-8 expression, which suggests HO-1 has a role in mediating the effects of tanshinone IIA. This study reports for the first time that tanshinone IIA inhibits cyclic strain-induced IL-8 expression via the induction of HO-1 in endothelial cells, providing valuable new insight into the molecular pathways that may contribute to the effects of tanshinone IIA.
authors.affiliation: * Deparment of Cardiology, Shanghai East Hospital.
authors.forename: Shaowei
authors.initials: S
authors.lastname: Zhuang
authors.affiliation: ‡ Department of Biochemistry, School of Medicine, College of Medicine.
authors.forename: Tzu-Hurng
authors.initials: TH
authors.lastname: Cheng
authors.affiliation: ¶ Department of Life Sciences, National University of Kaohsiung, Kaohsiung, Taiwan, R.O.C.
authors.forename: Nang-Lang
authors.initials: NL
authors.lastname: Shih
authors.affiliation: ∥ Division of Cardiology, Department of Internal Medicine, Shuang Ho Hospital Taipei Medical University, New Taipei City, Taiwan, R.O.C.
authors.forename: Ju-Chi
authors.initials: JC
authors.lastname: Liu
authors.affiliation: ** Graduate Institute of Clinical Medicine, College of Medicine, China Medical University Hospital, Taiwan, R.O.C.|†† Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, R.O.C.
authors.forename: Jin-Jer
authors.initials: JJ
authors.lastname: Chen
authors.affiliation: § School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung, Taiwan, R.O.C.
authors.forename: Hong-Jye
authors.initials: HJ
authors.lastname: Hong
authors.affiliation: † Shanghai East Taiwanese Hospital, Tongji University, Shanghai, P.R. China.|‡‡ Deparment of Cardiology, Taipei Medical University-Wan Fang Hospital, Taipei, Taiwan, R.O.C.
authors.forename: Paul
authors.initials: P
authors.lastname: Chan
mesh_terms: D001572:Benzofurans / Q000302:isolation & purification / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects* / Q000235:genetics*; D020868:Gene Silencing; D051547:Heme Oxygenase-1 / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D061307:Human Umbilical Vein Endothelial Cells / Q000201:enzymology* / Q000378:metabolism*; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics* / Q000378:metabolism*; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D027561:Salvia miltiorrhiza / Q000737:chemistry*
chemical_list: D001572:Benzofurans; D016209:Interleukin-8; D051267:NF-E2-Related Factor 2; C495635:NFE2L2 protein, human; C530742:tashinone IIA; D051547:Heme Oxygenase-1; D019869:Phosphatidylinositol 3-Kinases
keywords: Endothelial Cells; Heme Oxygenase-1; Interleukin-8; Tanshinone IIA; Traditional Chinese Medicine