title: Cell attachment and proliferation of osteoblast-like MG63 cells on silk fibroin membrane for guided bone regeneration.
abstract: BACKGROUND
  The aim of this study is to verify the feasibility of using silk fibroin (SF) as a potential membrane for guided bone regeneration (GBR).
  
  
  METHODS
  Various cellular responses (i.e., cell attachment, viability, and proliferation) of osteoblast-like MG63 cells cultured on an SF membrane were quantified. After culturing on an SF membrane for 1, 5, and 7 days, the attachment and surface morphology of MG63 cells were examined by optical and scanning electron microscopy (SEM), cell viability was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and cell proliferation was quantified using 4',6-diamidino-2-phenylindole (DAPI) fluorescence staining.
  
  
  RESULTS
  Optical microscopy revealed that MG63 cells cultured on the SF membrane proliferated over the 7-day observation period. The viability of cells cultured on SF membranes (SF group) and on control surfaces (control group) increased over time (P< 0.05); however, at respective time points, cell viability was not significantly different between the two groups (P> 0.05). In contrast, cell proliferation was significantly higher in the SF membrane group than in the control group at 7 days (P< 0.05).
  
  
  CONCLUSIONS
  These results suggest that silk fibroin is a biocompatible material that could be used as a suitable alternative barrier membrane for GBR.
authors.affiliation: Department of Translational Medicine, Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, South Korea.
authors.forename: Chae-Kyung
authors.initials: CK
authors.lastname: Yoo
authors.affiliation: Department of Dentistry/Division of Oral and Maxillofacial Surgery, College of Medicine, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul, 133-791 South Korea.
authors.forename: Jae-Yun
authors.initials: JY
authors.lastname: Jeon
authors.affiliation: Korean Minjok Leadership Academy, Hoengseong, South Korea.
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University, Gangneung, South Korea.
authors.forename: Seong-Gon
authors.initials: SG
authors.lastname: Kim
authors.affiliation: Department of Translational Medicine, Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, South Korea.|Department of Dentistry/Division of Oral and Maxillofacial Surgery, College of Medicine, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul, 133-791 South Korea.
authors.forename: Kyung-Gyun
authors.initials: KG
authors.lastname: Hwang
keywords: Bone regeneration; Cell adhesion; Cell proliferation; Membrane; Osteoblasts; Silk fibroin