title: Multilayer engineered nanoliposomes as a novel tool for oral delivery of lipopeptide-based vaccines against group A Streptococcus.
abstract: To develop an oral nanovaccine delivery system for lipopeptide-based vaccine candidate against group A Streptococcus.
  
  
  
  Lipid-core peptide-1-loaded nanoliposomes were prepared as a template and coated with opposite-charged polyelectrolytes to produce particles with size <200 nm. Efficacy of this oral nanovaccine delivery system was evaluated in mice model.
  
  
  
  Polymer-coated liposomes produced significantly higher antigen-specific mucosal IgA and systemic IgG titers in comparison to vaccine formulated with a strong mucosal adjuvant upon oral immunization in mice. Moreover, high levels of systemic antibody titers were retained even at day 185 postprimary immunization.
  
  
  
  Efficient oral delivery platform for lipopeptide-based vaccines has been developed.
authors.affiliation: School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia.
authors.forename: Nirmal
authors.initials: N
authors.lastname: Marasini
authors.affiliation: School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia.
authors.forename: Ashwini K
authors.initials: AK
authors.lastname: Giddam
authors.affiliation: School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia.
authors.forename: Khairunnisa A
authors.initials: KA
authors.lastname: Ghaffar
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast, QLD, Australia.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Batzloff
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast, QLD, Australia.
authors.forename: Michael F
authors.initials: MF
authors.lastname: Good
authors.affiliation: School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Skwarczynski
authors.affiliation: School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia.|School of Pharmacy, The University of Queensland, Woolloongabba, QLD 4102, Australia.|Institute for Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia.
authors.forename: Istvan
authors.initials: I
authors.lastname: Toth
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000917:Antibody Formation; D017951:Antigen Presentation; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D055666:Lipopeptides / Q000008:administration & dosage* / Q000276:immunology / Q000627:therapeutic use; D008081:Liposomes / Q000737:chemistry*; D051379:Mice; D049329:Nanostructures / Q000737:chemistry*; D000071228:Polyelectrolytes / Q000737:chemistry; D013290:Streptococcal Infections / Q000276:immunology / Q000517:prevention & control*; D022541:Streptococcal Vaccines / Q000008:administration & dosage* / Q000276:immunology / Q000627:therapeutic use; D013291:Streptococcus / Q000276:immunology*; D014611:Vaccination
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G; D055666:Lipopeptides; D008081:Liposomes; D000071228:Polyelectrolytes; D022541:Streptococcal Vaccines
keywords: group A Streptococcus; lipid-core peptide; liposomes; mucosal immunology; nanoparticles; oral delivery; peptides; vaccines