title: The ratio of CD4/CD8 T-cells in human papillomavirus-positive laryngeal squamous cell carcinoma accounts for improved outcome.
abstract: CONCLUSIONS
  Improved prognosis associated with HPV-positive status may depend on lower CD4/CD8 ratio. Th1 CD4(+ )T cells were found to be the major sub-set of T lymphocytes in the HPV positive laryngeal squamous cell carcinoma microenvironment.
  
  
  BACKGROUND
  To examine the prognostic significance of human papillomavirus (HPV) status in relation to the ratio of CD4/CD8 in LSCC.
  
  
  METHODS
  In this study, 46 LSCC biopsy samples were retrospectively assessed using immunohistochemistry for CD4(+ )and CD8(+ )tumor infiltrating lymphocytes (TILs). HPV status was determined by HPV in situ hybridization (ISH) and p16(INK4A) immunohistochemistry. Of the 46 samples, 21 were evaluated for the expression of IFN-γ and IL-4 by quantitative real-time PCR (qRT-PCR). The influence of HPV status on locoregional tumor control and T-cell sub-sets infiltrating tumor microenvironment were investigated.
  
  
  RESULTS
  Nineteen patients (41.3%) were classified as HPV positive, who had improved disease-free survival (28% in reduction, hazard ratio =0.10; 95% CI =0.011-0.938). A direct correlation between the HPV status and the ratio of CD4/CD8 or mean levels of CD8(+ )T cells was observed. Compared with the HPV-negative samples, HPV-positive samples had a higher ratio of IFN-γ/IL-4 (24.43 ± 29.89 vs 3.90 ± 4.03; p = 0.0375).
authors.affiliation: a Department of Otolaryngology-HNS , Eye, Ear, Nose and Throat Hospital, Shanghai Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of Medicine , Shanghai , PR China ;|b Department of Pudong Hospital , Fudan University School of Medicine , Shanghai , PR China ;
authors.forename: Duo
authors.initials: D
authors.lastname: Zhang
authors.affiliation: a Department of Otolaryngology-HNS , Eye, Ear, Nose and Throat Hospital, Shanghai Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of Medicine , Shanghai , PR China ;|b Department of Pudong Hospital , Fudan University School of Medicine , Shanghai , PR China ;
authors.forename: Wei-Jing
authors.initials: WJ
authors.lastname: Tang
authors.affiliation: a Department of Otolaryngology-HNS , Eye, Ear, Nose and Throat Hospital, Shanghai Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of Medicine , Shanghai , PR China ;|b Department of Pudong Hospital , Fudan University School of Medicine , Shanghai , PR China ;
authors.forename: Di
authors.initials: D
authors.lastname: Tang
authors.affiliation: a Department of Otolaryngology-HNS , Eye, Ear, Nose and Throat Hospital, Shanghai Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of Medicine , Shanghai , PR China ;|b Department of Pudong Hospital , Fudan University School of Medicine , Shanghai , PR China ;
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.affiliation: a Department of Otolaryngology-HNS , Eye, Ear, Nose and Throat Hospital, Shanghai Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of Medicine , Shanghai , PR China ;|b Department of Pudong Hospital , Fudan University School of Medicine , Shanghai , PR China ;
authors.forename: Liang
authors.initials: L
authors.lastname: Chou
authors.affiliation: a Department of Otolaryngology-HNS , Eye, Ear, Nose and Throat Hospital, Shanghai Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of Medicine , Shanghai , PR China ;|b Department of Pudong Hospital , Fudan University School of Medicine , Shanghai , PR China ;
authors.forename: Lei
authors.initials: L
authors.lastname: Tao
authors.affiliation: c Shanghai Institute of Immunology , Shanghai Jiaotong University School of Medicine , Shanghai , PR China.
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Lu
mesh_terms: D016516:CD4-CD8 Ratio; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000276:immunology* / Q000628:therapy / Q000821:virology; D016158:Genes, p53; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000276:immunology* / Q000628:therapy / Q000821:virology; D008875:Middle Aged; D027383:Papillomaviridae / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000150:complications*; D012189:Retrospective Studies; D058408:Th1-Th2 Balance
keywords: CD4/CD8; HPV; IFN-γ/IL-4; LSCC; TILs