title: The Influence of Alcohol Consumption in Conjunction with Sex Hormone Deficiency on Ca/P Ratio in Rats.
abstract: Deficiency of sex hormones and excessive alcohol consumption are factors that have been related to alterations in the pattern of bone mineralization and osteoporosis. The aim of this study was to evaluate possible alterations in the calcium/phosphorus (Ca/P) ratio in the femur of rats subjected to sex hormone deficiency and/or alcohol consumption. Methods. Female and male Wistar rats (n = 108) were divided into ovariectomized (Ovx), orchiectomized (Orx), or sham-operated groups and subdivided according to diet: alcoholic diet (20% alcohol solution), isocaloric diet, and ad libitum diet. The diets were administered for 8 weeks. The Ca/P ratio in the femur was analyzed by energy dispersive micro-X-ray spectrometer (μEDX). Results. Consumption of alcohol reduced the Ca/P ratio in both females and males. The isocaloric diet reduced the Ca/P ratio in females. In groups with the ad libitum diet, the deficiency of sex hormones did not change the Ca/P ratio in females or males. However, the combination of sex hormone deficiency and alcoholic diet presented the lowest values for the Ca/P ratio in both females and males. Conclusions. There was a reduced Ca/P ratio in the femur of rats that consumed alcohol, which was exacerbated when combined with a deficiency of sex hormones.
authors.affiliation: Institute of Science and Technology, Department of Biosciences and Diagnostics, Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José Longo 777, 12245-000 São José dos Campos, SP, Brazil.
authors.forename: Karina Bortolin
authors.initials: KB
authors.lastname: Lodi
authors.affiliation: Institute of Science and Technology, Department of Biosciences and Diagnostics, Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José Longo 777, 12245-000 São José dos Campos, SP, Brazil.
authors.forename: Adriana Mathias Pereira da Silva
authors.initials: AM
authors.lastname: Marchini
authors.affiliation: Institute of Environmental Science, Chemical and Pharmaceutical, Department of Mathematical and Earth Sciences, Federal University of São Paulo (UNIFESP), Rua Prof. Artur Riedel 275, 09972-270 Diadema, SP, Brazil; Institute of Research and Development, University of Vale do Paraíba (UNIVAP), Laboratory of Biomedical Vibrational Spectroscopy, Avenida Shishima Hifumi 2911, 12244-000 São José dos Campos, SP, Brazil.
authors.forename: Ana Maria do Espírito
authors.initials: AM
authors.lastname: Santo
authors.affiliation: Institute of Science and Technology, Department of Dental Materials and Prosthesis, Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José Longo 777, 12245-000 São José dos Campos, SP, Brazil.
authors.forename: Sigmar de Mello
authors.initials: Sde M
authors.lastname: Rode
authors.affiliation: Department of Preventive and Community Dentistry, The University of Iowa College of Dentistry and Dental Clinics, N337-1 Dental Science N, 52242 Iowa City, IA, USA.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Marchini
authors.affiliation: Institute of Science and Technology, Department of Biosciences and Diagnostics, Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José Longo 777, 12245-000 São José dos Campos, SP, Brazil.
authors.forename: Rosilene Fernandes
authors.initials: RF
authors.lastname: da Rocha