title: Structural characterization, α-glucosidase inhibitory and DPPH scavenging activities of polysaccharides from guava.
abstract: To explore the chemicals responsible for the health benefits of guava, water-soluble polysaccharides were extracted including GP90 and P90. They exhibited excellent α-glucosidase inhibition activity with an EC50 of 2.27μg/mL and 0.18mg/mL. This suggests that their activities were 1379- and 17-fold higher than the positive control. The DPPH scavenging activities of GP90 was even higher than Vc at some concentrations. Upon further isolation, a novel polysaccharide termed GB90-1B was obtained. Monosaccharide analysis, methylation analysis, and NMR were used to analyze the structural characterization of GB90-1B. Structural analysis revealed that its backbone consisted of (1→5)-linked-α-l-arabinose, (1→2,3,5)-linked-α-l-arabinose and (1→3)-linked-α-l-arabinose. Branch linkages included (1→6)-linked-α-d-glucose, (1→)-linked-α-d-glucose and (1→)-linked-α-l-arabinose. The structure of the repeating unit of GP90-1B was predicted.
authors.affiliation: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China; Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of TCM, Guangzhou, China; Engineering & Technology Research Center for Chinese Materia Medica Quality of the Universities of Guangdong Province, Guangzhou 510006, China.
authors.forename: Ziling
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Fansheng
authors.initials: F
authors.lastname: Kong
authors.affiliation: XinJiang Institute of Chinese Materia Medica and Ethnodrug, Urumqi 830002, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ni
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Zhixian
authors.initials: Z
authors.lastname: Mo
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Wan
authors.affiliation: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Dehong
authors.initials: D
authors.lastname: Hua
authors.affiliation: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China. Electronic address: ycybridge@163.com.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Yan
mesh_terms: D020909:Acarbose / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry*; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D016166:Free Radical Scavengers / Q000737:chemistry*; D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D010851:Picrates / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D066244:Proton Magnetic Resonance Spectroscopy; D031647:Psidium / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D000520:alpha-Glucosidases / Q000737:chemistry*
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; C000620796:GP90-1B polysaccharide; D065089:Glycoside Hydrolase Inhibitors; D010851:Picrates; D011134:Polysaccharides; C004931:1,1-diphenyl-2-picrylhydrazyl; D000520:alpha-Glucosidases; D020909:Acarbose
keywords: 1,1-diphenyl-2-picrylhydrazyl (PubChem CID:2735032); 1-phenyl-3-methyl-5-pyrazolone (PubChem CID:70335); 4-nitrophenyl-α-d-glucopyranoside (PubChem CID: 259380); Butanol (PubChem CID: 263); Chloroform (PubChem CID: 6212); DPPH scavenging activity; Dimethyl sulfoxide (PubChem CID: 679); Ethanol (PubChem CID: 702); Guava; Polysaccharides; Psidium guajava Linn.; Sodium chloride (PubChem CID: 5234); Sodium hydroxide (PubChem CID:14798); Structure; Trifluoroacetic acid (PubChem CID: 6422); α-Glucosidase inhibition activity