title: Influence of sex on aquaporin1-4 and vasopressin V2 receptor expression in the pig kidney during development.
abstract: The ability of the immature kidney to concentrate urine is lower than in adults. The aquaporin (AQP) family and the vasopressin V2 receptor (V2R) play a critical role in the urinary concentrating capacity. Here we investigated a possible sex difference in AQP1, AQP2, AQP3, and AQP4 as well as V2R expression in the fetal pig kidney at different gestation stages.
  
  
  
  Pig fetuses were divided into three groups according to gestation age of 60, 80, and 100 d. Quantitative PCR and immunohistochemistry were used to determine the regulation of AQP1, AQP2, AQP3, and AQP4 as well as V2R in the fetal pig kidneys.
  
  
  
  Renal AQP1, AQP2 and AQP3, and V2R expression was increased with gestation age in both sexes, whereas AQP4 expression was unchanged over time. We observed neither sex differences in the AQPs nor V2R expression in the fetal pig kidneys.
  
  
  
  AQP1, AQP2, and AQP3, and V2R expression increased with gestation age in the fetal kidney, suggesting that this induction might contribute to the maturation of urinary concentrating capacity. However, no sex differences were observed indicating that sex might not play a role for the maturation of the urinary concentrating activity during kidney development in fetal pig.
authors.affiliation: Department of Clinical Medicine, Aarhus University, Aarhus, Denmark.
authors.forename: Lu
authors.initials: L
authors.lastname: Xing
authors.affiliation: Department of Clinical Medicine, Aarhus University, Aarhus, Denmark.
authors.forename: Rikke
authors.initials: R
authors.lastname: Nørregaard
mesh_terms: D000818:Animals; D051398:Aquaporin 1 / Q000378:metabolism*; D051399:Aquaporin 2 / Q000378:metabolism*; D051400:Aquaporin 3 / Q000378:metabolism*; D051401:Aquaporin 4 / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006461:Hemolysis; D007668:Kidney / Q000196:embryology* / Q000378:metabolism; D008297:Male; D009994:Osmolar Concentration; D017483:Receptors, Vasopressin / Q000378:metabolism*; D012737:Sex Factors; D013552:Swine; D013997:Time Factors
chemical_list: D051399:Aquaporin 2; D051401:Aquaporin 4; D017483:Receptors, Vasopressin; D051398:Aquaporin 1; D051400:Aquaporin 3