title: The resistance and transmission mechanism of Acinetobacter baumannii isolates in a tertiary care hospital, China.
abstract: The aim of this study was to analyse the resistance and epidemiological data of 117 Acinetobacter baumannii isolates from Southwest Hospital, Chongqing, China. Except for polymyxin B, tigecycline, minocycline, cefoperazone/sulbactam, amikacin and levofloxacin, the resistance rates of other antimicrobial agents were above 90%. All the clinical isolates had the blaOXA-51gene and 114 isolates had the blaOXA-23gene. Forty-nine isolates were found to contain the blaIMP-4gene. PFGE data showed that 117 isolates were divided into 25 groups. Sixty-three (53.85%) were found to carry the class 1 integron, and the sequence analysis of the class 1 integron internal variable regions - five types, one of which had the blaIMP-4gene. For the blaIMP-4positive strain without class 1 integron, we found the flanking sequence had the TnpA gene. The result suggested that the resistance gene was widely distributed in our hospital; moreover, the modes of presence and transmission are different and complicated. The results of our study can improve the infection empirical treatment method and infection control programme.
authors.affiliation: a Department of Pharmacy, Southwest Hospital , Third Military Medical University , Chongqing , China.
authors.forename: Fengjun
authors.initials: F
authors.lastname: Sun
authors.affiliation: a Department of Pharmacy, Southwest Hospital , Third Military Medical University , Chongqing , China.
authors.forename: Qianyi
authors.initials: Q
authors.lastname: Ou
authors.affiliation: b Department of Pharmacy, Xinqiao Hospital , Third Military Medical University , Chongqing , China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: a Department of Pharmacy, Southwest Hospital , Third Military Medical University , Chongqing , China.
authors.forename: Wei
authors.initials: W
authors.lastname: Feng
authors.affiliation: a Department of Pharmacy, Southwest Hospital , Third Military Medical University , Chongqing , China.
authors.forename: Xuewen
authors.initials: X
authors.lastname: Qiu
authors.affiliation: a Department of Pharmacy, Southwest Hospital , Third Military Medical University , Chongqing , China.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Chen
authors.affiliation: a Department of Pharmacy, Southwest Hospital , Third Military Medical University , Chongqing , China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Liu
authors.affiliation: a Department of Pharmacy, Southwest Hospital , Third Military Medical University , Chongqing , China.
authors.forename: Peiyuan
authors.initials: P
authors.lastname: Xia
mesh_terms: D000151:Acinetobacter Infections / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission; D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D002681:China; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006801:Humans; D062606:Tertiary Care Centers
keywords: Acinetobacter baumannii; Pulsed-field gel electrophoresis; Resistance; Transmission mechanism