title: Vector Surveillance for Dengue Virus Detection in the Archipelago of Fernando de Noronha, Brazil.
abstract: Aedes aegypti (L.) has become an efficient vector of important arboviruses due to its anthropophilic and domiciliary behaviors. Since the 1980s, dengue affects thousands of people every year in Brazil; in Fernando de Noronha (FN), a touristic archipelago, dengue cases have occurred since 2001. Once Ae. aegypti populations are well established in the inhabited areas of FN, the threat of dengue or another arbovirus epidemic is continuously imminent. This study aimed to monitor the DENV serotypes in mosquito samples collected in FN, where at least one resident was clinically diagnosed as dengue patient. Entomological surveillance was conducted in 2011 and 2012. Mosquitoes were sorted by sex and location and were stored in pools. DENV detection was performed using polymerase chain reaction with reverse transcription (RT-PCR) and the Platelia Dengue NS1 Ag. RNA integrity was checked by RT-PCR using rpL8 primers, and the minimum infection rate (MIR) was calculated. In total, 339 pools were analyzed, and only one was positive (DENV-1) by Multiplex RT-PCR (MIR = 1.53). When considering only pools with RNA integrity, the MIR was 2.92. Using the Platelia kit, the MIR was 9.18 (considering all the pools) and 17.54 (only 140 pools with RNA integrity). Our results showed the importance of a constant entomological surveillance in that area, the need to improve storage and transportation protocols, and an endogenous control in the RT-PCR to avoid false-negative results. Finally, our study indicated that the NS1-Ag detection was the most sensitive method and should be used routinely for DENV surveillance in mosquitoes if the serotype identification is not required.
authors.affiliation: Departamento de Entomologia, Centro de Pesquisas Aggeu Magalhães/Fundação Oswaldo Cruz (CPqAM/FIOCRUZ-PE), Av. Professor Moraes Rego s/n, Cidade Universitária, Recife PE, Brazil (priscillapaschoal@hotmail.com; duschinka@gmail.com; mavarjal@gmail.com; regis.leda@gmail.com; tans@cpqam.fiocruz.br).
authors.forename: P P
authors.initials: PP
authors.lastname: Barbosa
authors.affiliation: Departamento de Entomologia, Centro de Pesquisas Aggeu Magalhães/Fundação Oswaldo Cruz (CPqAM/FIOCRUZ-PE), Av. Professor Moraes Rego s/n, Cidade Universitária, Recife PE, Brazil (priscillapaschoal@hotmail.com; duschinka@gmail.com; mavarjal@gmail.com; regis.leda@gmail.com; tans@cpqam.fiocruz.br).
authors.forename: D R D
authors.initials: DR
authors.lastname: Guedes
authors.affiliation: Departamento de Entomologia, Centro de Pesquisas Aggeu Magalhães/Fundação Oswaldo Cruz (CPqAM/FIOCRUZ-PE), Av. Professor Moraes Rego s/n, Cidade Universitária, Recife PE, Brazil (priscillapaschoal@hotmail.com; duschinka@gmail.com; mavarjal@gmail.com; regis.leda@gmail.com; tans@cpqam.fiocruz.br).
authors.forename: M A V
authors.initials: MA
authors.lastname: Melo-Santos
authors.affiliation: Departamento de Virologia, Laboratório de Virologia e Terapia Experimental - LaViTE, Centro de Pesquisas Aggeu Magalhães/Fundação Oswaldo Cruz (CPqAM/FIOCRUZ-PE), Av. Professor Moraes Rego s/n, Cidade Universitária, Recife PE, Brazil (marli@cpqam.fiocruz.br).
authors.forename: M T
authors.initials: MT
authors.lastname: Cordeiro
authors.affiliation: Secretaria de Saúde do Estado de Pernambuco em Fernando de Noronha (ridelane.veiga@hotmail.com; carlosvbatista@ig.com.br; liliane.melo@hotmail.com; fatimamsouza@hotmail.com; yedalima@hotmail.com; fernando.rodrigues@noronha.pe.gov.br), and.
authors.forename: R V
authors.initials: RV
authors.lastname: Acioli
authors.affiliation: Secretaria de Saúde do Estado de Pernambuco em Fernando de Noronha (ridelane.veiga@hotmail.com; carlosvbatista@ig.com.br; liliane.melo@hotmail.com; fatimamsouza@hotmail.com; yedalima@hotmail.com; fernando.rodrigues@noronha.pe.gov.br), and.
authors.forename: C A V
authors.initials: CA
authors.lastname: Batista
authors.affiliation: Secretaria de Saúde do Estado de Pernambuco em Fernando de Noronha (ridelane.veiga@hotmail.com; carlosvbatista@ig.com.br; liliane.melo@hotmail.com; fatimamsouza@hotmail.com; yedalima@hotmail.com; fernando.rodrigues@noronha.pe.gov.br), and.
authors.forename: L S M
authors.initials: LS
authors.lastname: Gonçalves
authors.affiliation: Secretaria de Saúde do Estado de Pernambuco em Fernando de Noronha (ridelane.veiga@hotmail.com; carlosvbatista@ig.com.br; liliane.melo@hotmail.com; fatimamsouza@hotmail.com; yedalima@hotmail.com; fernando.rodrigues@noronha.pe.gov.br), and.
authors.forename: M F M
authors.initials: MF
authors.lastname: Souza
authors.affiliation: Secretaria de Saúde do Estado de Pernambuco em Fernando de Noronha (ridelane.veiga@hotmail.com; carlosvbatista@ig.com.br; liliane.melo@hotmail.com; fatimamsouza@hotmail.com; yedalima@hotmail.com; fernando.rodrigues@noronha.pe.gov.br), and.
authors.forename: Y V
authors.initials: YV
authors.lastname: Araújo
authors.affiliation: Secretaria de Saúde do Estado de Pernambuco em Fernando de Noronha (ridelane.veiga@hotmail.com; carlosvbatista@ig.com.br; liliane.melo@hotmail.com; fatimamsouza@hotmail.com; yedalima@hotmail.com; fernando.rodrigues@noronha.pe.gov.br), and.
authors.forename: F J R
authors.initials: FJ
authors.lastname: Magalhães
authors.affiliation: Departamento de Entomologia, Centro de Pesquisas Aggeu Magalhães/Fundação Oswaldo Cruz (CPqAM/FIOCRUZ-PE), Av. Professor Moraes Rego s/n, Cidade Universitária, Recife PE, Brazil (priscillapaschoal@hotmail.com; duschinka@gmail.com; mavarjal@gmail.com; regis.leda@gmail.com; tans@cpqam.fiocruz.br).
authors.forename: L
authors.initials: L
authors.lastname: Regis
authors.affiliation: Departamento de Entomologia, Centro de Pesquisas Aggeu Magalhães/Fundação Oswaldo Cruz (CPqAM/FIOCRUZ-PE), Av. Professor Moraes Rego s/n, Cidade Universitária, Recife PE, Brazil (priscillapaschoal@hotmail.com; duschinka@gmail.com; mavarjal@gmail.com; regis.leda@gmail.com; tans@cpqam.fiocruz.br), tans@cpqam.fiocruz.br.
authors.forename: C F J
authors.initials: CF
authors.lastname: Ayres
mesh_terms: D000330:Aedes / Q000502:physiology / Q000821:virology*; D000818:Animals; D001938:Brazil / Q000453:epidemiology; D003715:Dengue / Q000453:epidemiology / Q000635:transmission / Q000821:virology*; D003716:Dengue Virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D005260:Female; D006801:Humans; D007303:Insect Vectors / Q000502:physiology / Q000821:virology; D008297:Male; D018571:Sentinel Surveillance; D065288:Serogroup
keywords: Fernando de Noronha; NS1; RT-PCR; arbovirus; diagnostic