title: Production of Succinate from Acetate by Metabolically Engineered Escherichia coli.
abstract: Acetate, a major component of industrial biological wastewater and of lignocellulosic biomass hydrolysate, could potentially be a less costly alternative carbon source. Here we engineered Escherichia coli MG1655 strain for succinate production from acetate as the sole carbon source. Strategies of metabolic engineering included the blockage of the TCA cycle, redirection of the gluconeogenesis pathway, and enhancement of the glyoxylate shunt. The engineered strain MG03 featuring the deletion of genes: succinate dehydrogenase (sdhAB), isocitrate lyase regulator (iclR), and malic enzymes (maeB) accumulated 6.86 mM of succinate in 72 h. MG03(pTrc99a-gltA) overexpressing citrate synthase (gltA) accumulated 16.45 mM of succinate and the yield reached 0.46 mol/mol, about 92% of the maximum theoretical yield. Resting-cell was adopted for the conversion of acetate to succinate, and the highest concentration of succinate achieved 61.7 mM.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , 130 Meilong Road, Shanghai 200237, China.|Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , 32 West seventh Avenue, Tianjin Airport Economic Area, Tianjin 300308, China.
authors.forename: Yunjie
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , 130 Meilong Road, Shanghai 200237, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , 130 Meilong Road, Shanghai 200237, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , 130 Meilong Road, Shanghai 200237, China.|Shanghai Collaborative Innovation Center for Biomanufacturing Technology , 130 Meilong Road, Shanghai 200237, China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , 130 Meilong Road, Shanghai 200237, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Ye
authors.affiliation: Biological Systems Engineering Department, Virginia Tech , 304 Seitz Hall, Blacksburg, Virginia 24061, United States.|Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , 32 West seventh Avenue, Tianjin Airport Economic Area, Tianjin 300308, China.
authors.forename: Y-H Percival
authors.initials: YP
authors.lastname: Zhang
mesh_terms: D000085:Acetates / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002950:Citrate (si)-Synthase / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D020869:Gene Expression Profiling; D006038:Glyoxylates / Q000378:metabolism; D007522:Isocitrate Lyase / Q000235:genetics / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D060847:Metabolic Engineering; D000074041:Microorganisms, Genetically-Modified; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017423:Sequence Analysis, RNA; D013385:Succinate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D000085:Acetates; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D006038:Glyoxylates; D012097:Repressor Proteins; C067479:IclR protein, E coli; D019802:Succinic Acid; D008291:Malate Dehydrogenase; D013385:Succinate Dehydrogenase; D002950:Citrate (si)-Synthase; D007522:Isocitrate Lyase; C031150:glyoxylic acid
keywords: Escherichia coli; acetate; citrate synthase; malic enzyme; metabolic engineering; succinate