title: Bavachin from Psoralea corylifolia Improves Insulin-Dependent Glucose Uptake through Insulin Signaling and AMPK Activation in 3T3-L1 Adipocytes.
abstract: The fruit of Psoralea corylifolia L. (Fabaceae) (PC), known as "Bo-Gol-Zhee" in Korea has been used as traditional medicine. Ethanol and aqueous extracts of PC have an anti-hyperglycemic effect by increasing plasma insulin levels and decreasing blood glucose and total plasma cholesterol levels in type 2 diabetic rats. In this study, we purified six compounds from PC and investigated their anti-diabetic effect. Among the purified compounds, bavachin most potently accumulated lipids during adipocyte differentiation. Intracellular lipid accumulation was measured by Oil Red-O (ORO) cell staining to investigate the effect of compounds on adipogenesis. Consistently, bavachin activated gene expression of adipogenic transcriptional factors, proliferator-activated receptorγ (PPARγ) and CCAAT/enhancer binding protein-α (C/EBPα). Bavachin also increased adiponectin expression and secretion in adipocytes. Moreover, bavachin increased insulin-induced glucose uptake by differentiated adipocytes and myoblasts. In differentiated adipocytes, we found that bavachin enhanced glucose uptake via glucose transporter 4 (GLUT4) translocation by activating the Akt and 5'AMP-activated protein kinase (AMPK) pathway in the presence or absence of insulin. These results suggest that bavachin from Psoralea corylifolia might have therapeutic potential for type 2 diabetes by activating insulin signaling pathways.
authors.affiliation: College of Pharmacy and Research Center for Cell Fate Control, Sookmyung Women's University, 52 Hyochangwongil, Yongsan-gu, Seoul 140-742, Korea. u9698115@naver.com.
authors.forename: Hyejin
authors.initials: H
authors.lastname: Lee
authors.affiliation: College of Pharmacy and Research Center for Cell Fate Control, Sookmyung Women's University, 52 Hyochangwongil, Yongsan-gu, Seoul 140-742, Korea. cooldog227@hotmail.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.affiliation: Natural Products Research Institute, College of Pharmacy, Seoul National University, 1 Gwanak-ro, Gwanak-Gu, Seoul 151-742, Korea. minsoonoh@snu.ac.kr.
authors.forename: Minsoo
authors.initials: M
authors.lastname: Noh
authors.affiliation: College of Pharmacy and Research Center for Cell Fate Control, Sookmyung Women's University, 52 Hyochangwongil, Yongsan-gu, Seoul 140-742, Korea. ryuha@sookmyung.ac.kr.
authors.forename: Jae-Ha
authors.initials: JH
authors.lastname: Ryu
mesh_terms: D041721:3T3-L1 Cells; D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects*; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D051275:Glucose Transporter Type 4 / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D051379:Mice; D047495:PPAR gamma / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D029907:Psoralea / Q000737:chemistry; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D005419:Flavonoids; D051275:Glucose Transporter Type 4; D007004:Hypoglycemic Agents; D007328:Insulin; D047495:PPAR gamma; C459212:bavachin; D051057:Proto-Oncogene Proteins c-akt; D055372:AMP-Activated Protein Kinases; D005947:Glucose
keywords: 3T3-L1; Psoralea corylifolia; adipogenesis; diabetes mellitus; glucose uptake