title: Adsorbing/dissolving Lyoprotectant Matrix Technology for Non-cryogenic Storage of Archival Human Sera.
abstract: Despite abundant research conducted on cancer biomarker discovery and validation, to date, less than two-dozen biomarkers have been approved by the FDA for clinical use. One main reason is attributed to inadvertent use of low quality biospecimens in biomarker research. Most proteinaceous biomarkers are extremely susceptible to pre-analytical factors such as collection, processing, and storage. For example, cryogenic storage imposes very harsh chemical, physical, and mechanical stresses on biospecimens, significantly compromising sample quality. In this communication, we report the development of an electrospun lyoprotectant matrix and isothermal vitrification methodology for non-cryogenic stabilization and storage of liquid biospecimens. The lyoprotectant matrix was mainly composed of trehalose and dextran (and various low concentration excipients targeting different mechanisms of damage), and it was engineered to minimize heterogeneity during vitrification. The technology was validated using five biomarkers; LDH, CRP, PSA, MMP-7, and C3a. Complete recovery of LDH, CRP, and PSA levels was achieved post-rehydration while more than 90% recovery was accomplished for MMP-7 and C3a, showing promise for isothermal vitrification as a safe, efficient, and low-cost alternative to cryogenic storage.
authors.affiliation: Biostabilization Laboratory, Department of Mechanical Engineering, University of Minnesota, Minneapolis, MN 55455, USA.
authors.forename: Morwena J
authors.initials: MJ
authors.lastname: Solivio
authors.affiliation: Biostabilization Laboratory, Department of Mechanical Engineering, University of Minnesota, Minneapolis, MN 55455, USA.|School of Biomedical Engineering and Sciences, Virginia Tech, Blacksburg, VA 24061, USA.
authors.forename: Rebekah
authors.initials: R
authors.lastname: Less
authors.affiliation: Biostabilization Laboratory, Department of Mechanical Engineering, University of Minnesota, Minneapolis, MN 55455, USA.
authors.forename: Mathew L
authors.initials: ML
authors.lastname: Rynes
authors.affiliation: Biostabilization Laboratory, Department of Mechanical Engineering, University of Minnesota, Minneapolis, MN 55455, USA.
authors.forename: Marcus
authors.initials: M
authors.lastname: Kramer
authors.affiliation: Biostabilization Laboratory, Department of Mechanical Engineering, University of Minnesota, Minneapolis, MN 55455, USA.
authors.forename: Alptekin
authors.initials: A
authors.lastname: Aksan
mesh_terms: D001798:Blood Proteins / Q000032:analysis*; D006801:Humans; D011309:Preservation, Biological / Q000379:methods*; D044967:Serum / Q000737:chemistry*; D058989:Vitrification
chemical_list: D001798:Blood Proteins