title: Inhibitory effects of laminaran and alginate on production of putrefactive compounds from soy protein by intestinal microbiota in vitro and in rats.
abstract: Soybean is one of the major components of the Japanese diet. In traditional Japanese cuisine, soybean-based food items are often consumed with brown algae. In this study, we examined the effect of water-soluble and fermentable polysaccharides, laminaran and sodium alginate, from brown algae, on putrefactive compound production, by human faecal microbiota in broth containing 3% (w/v) soy protein. We also investigated the effect of 2% laminaran or alginate diet on caecal putrefactive compounds in rats maintained on diets containing 20% (w/w) soy protein. The caecal microbiota was also analysed using denaturing gradient gel electrophoresis and pyrosequencing with primers targeting the bacterial 16S rRNA gene. The polysaccharides, particularly laminaran, inhibited ammonia, phenol, and indole production by human faecal microbiota. Both the algal polysaccharides lowered the caecal indole content. Laminaran was found to increase the number of Coprobacter, whereas Helicobacter was found to decrease in the presence of both laminaran and sodium alginate.
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-city, Tokyo 108-8477, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Nakata
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-city, Tokyo 108-8477, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kyoui
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-city, Tokyo 108-8477, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-city, Tokyo 108-8477, Japan.
authors.forename: Bon
authors.initials: B
authors.lastname: Kimura
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-city, Tokyo 108-8477, Japan. Electronic address: kuda@kaiyodai.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kuda
mesh_terms: D000085:Acetates / Q000378:metabolism; D000328:Adult; D000464:Alginates / Q000494:pharmacology*; D000641:Ammonia / Q000037:antagonists & inhibitors / Q000378:metabolism; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D005243:Feces / Q000382:microbiology; D005285:Fermentation; D000069196:Gastrointestinal Microbiome / Q000502:physiology*; D005936:Glucans / Q000494:pharmacology*; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007211:Indoles / Q000037:antagonists & inhibitors* / Q000378:metabolism; D019344:Lactic Acid / Q000096:biosynthesis; D008297:Male; D009929:Organ Size / Q000187:drug effects; D000459:Phaeophyceae; D019800:Phenol / Q000037:antagonists & inhibitors / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017208:Rats, Wistar; D030262:Soybean Proteins / Q000008:administration & dosage / Q000378:metabolism*
chemical_list: D000085:Acetates; D000464:Alginates; D005936:Glucans; D006603:Hexuronic Acids; D007211:Indoles; D012336:RNA, Ribosomal, 16S; D030262:Soybean Proteins; D019800:Phenol; D019344:Lactic Acid; D000641:Ammonia; C030374:indole; D020723:Glucuronic Acid; C008247:laminaran
keywords: Intestinal environment; Intestinal microbiota; Laminaran; Rat; Sodium alginate; Soy protein