title: Cavernosum smooth muscle relaxation induced by Schisandrol A via the NO-cGMP signaling pathway.
abstract: To evaluate the effect of Schisandrol A on rabbit corpus cavernosum smooth muscle and elucidate the potential mechanism. Penises were obtained from healthy male New Zealand White rabbits (2.5-3.0 kg). The pre-contracted penis with phenylephrine (Phe, 10 µM) was treated with accumulative concentrations of Schisandrol A (10-7, 10-6, 10-5 and 10-4 M). The change in intracavernosum pressure (ICP) and tension was recorded, cyclic nucleotides in the cavernosum tissue were measured by radioimmunoassay, mRNA level and expression of endothelial nitric oxide synthase (eNOS) and neuronal NOS (nNOS) were measured by real time PCR and western blot respectively. The corpus cavernosum smooth muscle relaxation induced by Schisandrol A was in a dose-dependent manner. Pre-treatment with NOS inhibitor (Nω nitro-L-arginine-methyl ester, L-NAME) or guanylyl cyclase inhibitor (1H-(1,2,4)oxadiazolo(4,3-a)quinoxalin-1-one, ODQ) significantly diminished the relaxation. The cyclic guanosine monophosphate (cGMP) level was significantly increased in the cavernosum tissue. Real time PCR and western blot showed the mRNA level and expression of eNOS and nNOS was also upregulated. Schisandrol A relaxes the cavernosum smooth muscle by activating NO-cGMP signaling pathway. It may be a new promising treatment for erectile dysfunction and cardiovascular disease.
authors.affiliation: Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University and Shanghai Institute of Andrology Departments of Urology Shanghai China.
authors.forename: W
authors.initials: W
authors.lastname: Liu
authors.affiliation: Chonbuk National University Hospital Departments of Urology Jeonju South Korea.
authors.forename: B R
authors.initials: BR
authors.lastname: Choi
authors.affiliation: Chonbuk National University Hospital Departments of Urology Jeonju South Korea.
authors.forename: Y O
authors.initials: YO
authors.lastname: Bak
authors.affiliation: Chonbuk National University Hospital Departments of Urology Jeonju South Korea.
authors.forename: L T
authors.initials: LT
authors.lastname: Zhang
authors.affiliation: Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University and Shanghai Institute of Andrology Departments of Urology Shanghai China.
authors.forename: L X
authors.initials: LX
authors.lastname: Zhou
authors.affiliation: Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University and Shanghai Institute of Andrology Departments of Urology Shanghai China.
authors.forename: Y R
authors.initials: YR
authors.lastname: Huang
authors.affiliation: Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University and Shanghai Institute of Andrology Departments of Urology Shanghai China chenzhao1737@sina.com.
authors.forename: C
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Chonbuk National University Hospital Departments of Urology Jeonju South Korea jongkwanpark@sina.com.
authors.forename: J K
authors.initials: JK
authors.lastname: Park
mesh_terms: D000818:Animals; D006152:Cyclic GMP / Q000378:metabolism; D034242:Cyclooctanes / Q000737:chemistry / Q000494:pharmacology*; D007172:Erectile Dysfunction / Q000188:drug therapy* / Q000235:genetics / Q000503:physiopathology; D005786:Gene Expression Regulation / Q000187:drug effects; D017705:Lignans / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D009569:Nitric Oxide / Q000378:metabolism; D052248:Nitric Oxide Synthase Type I / Q000235:genetics; D052250:Nitric Oxide Synthase Type III / Q000235:genetics; D010413:Penis / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D011817:Rabbits; D032141:Schisandra / Q000737:chemistry; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D034242:Cyclooctanes; D017705:Lignans; C520474:schisandrol A; D009569:Nitric Oxide; D052248:Nitric Oxide Synthase Type I; D052250:Nitric Oxide Synthase Type III; D006152:Cyclic GMP