title: Optimization of SDS exposure on preservation of ECM characteristics in whole organ decellularization of rat kidneys.
abstract: Renal transplantation is well established as the optimal form of renal replacement therapy but is restricted by the limited pool of organs available for transplantation. The whole organ decellularisation approach is leading the way for a regenerative medicine solution towards bioengineered organ replacements. However, systematic preoptimization of both decellularization and recellularization parameters is essential prior to any potential clinical application and should be the next stage in the evolution of whole organ decellularization as a potential strategy for bioengineered organ replacements. Here we have systematically assessed two fundamental parameters (concentration and duration of perfusion) with regards to the effects of differing exposure to the most commonly used single decellularizing agent (sodium dodecyl sulphate/SDS) in the perfusion decellularization process for whole rat kidney ECM bioscaffolds, with findings showing improved preservation of both structural and functional components of the whole kidney ECM bioscaffold. Whole kidney bioscaffolds based on our enhanced protocol were successfully recellularized with rat primary renal cells and mesenchymal stromal cells. These findings should be widely applicable to decellularized whole organ bioscaffolds and their optimization in the development of regenerated organ replacements for transplantation. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1352-1360, 2017.
authors.affiliation: Department of Bioengineering, Imperial College London, London, UK.|Department of Materials and Institute of Biomedical Engineering, Imperial College London, London, UK.
authors.forename: M
authors.initials: M
authors.lastname: He
authors.affiliation: Institute for BioEngineering (IBioE), School of Engineering, University of Edinburgh, Edinburgh, UK.
authors.forename: A
authors.initials: A
authors.lastname: Callanan
authors.affiliation: Department of Bioengineering, Imperial College London, London, UK.|Department of Materials and Institute of Biomedical Engineering, Imperial College London, London, UK.
authors.forename: K
authors.initials: K
authors.lastname: Lagaras
authors.affiliation: Department of Bioengineering, Imperial College London, London, UK.|Department of Materials and Institute of Biomedical Engineering, Imperial College London, London, UK.
authors.forename: J A M
authors.initials: JAM
authors.lastname: Steele
authors.affiliation: Department of Bioengineering, Imperial College London, London, UK.|Department of Materials and Institute of Biomedical Engineering, Imperial College London, London, UK.
authors.forename: M M
authors.initials: MM
authors.lastname: Stevens
mesh_terms: D000818:Animals; D005109:Extracellular Matrix / Q000737:chemistry*; D007668:Kidney / Q000737:chemistry*; D008297:Male; D011309:Preservation, Biological; D051381:Rats; D017208:Rats, Wistar; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D012967:Sodium Dodecyl Sulfate
keywords: extracellular matrix; regenerative medicine; tissue engineering