title: Antifibrotic effect of dexamethasone/alginate-coated silicone sheet in the abraded middle ear mucosa.
abstract: Silicone sheet is a material which is commonly used in middle ear surgery to prevent the formation of adhesions between the tympanic membrane and the medial bony wall of the middle ear cavity. However, silicone sheet can induce a tight and hard fibrous capsule in the region of the stapes, and this is particularly common in cases of eustachian tube dysfunction. As a result of the fibrous encapsulation around the silicone sheet, postoperative aeration of the stapes can be interrupted causing poor hearing gain. In this study, we performed an in vitro and in vivo evaluation of the antifibrotic effects of a dexamethasone and alginate (Dx/alginate) coating on silicone sheet. The Dx/alginate-coated silicone sheets were fabricated using a plasma-treatment and coating method. The Dx/alginate-coated silicone sheets effectively limited in vitro fibroblast attachment and proliferation due to the controlled release of Dx, which can be modified by manipulation of the alginate coating. For the in-vivo evaluation, guinea pigs (albino, male, weighing 250g) were divided into two groups, with the control group (n=5) implanted with silicone sheet and the test group (n=5) receiving Dx/alginate-coated silicone sheet. Animals were sacrificed 3 weeks after implantation, and histological analysis was performed using hematoxylin and eosin (H&E) and immunohistochemical staining techniques. Dx/alginate-coated silicone sheets showed marked inhibition of fibrosis in both the in vitro and in vivo studies. Silicone sheet that incorporates a Dx/alginate coating can release Dx and inhibit fibrosis in the middle ear. This material could be utilized in middle ear surgery as a means of preserving proper aeration and hearing gain following ossiculoplasty.
authors.affiliation: Department of Otolaryngology, Chonnam National University Medical School, Gwangju, South Korea. Electronic address: chulsavio@hanmail.net.
authors.forename: Chul Ho
authors.initials: CH
authors.lastname: Jang
authors.affiliation: Department of Biomechatronics, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon 440-746, South Korea.
authors.forename: Seung Hyun
authors.initials: SH
authors.lastname: Ahn
authors.affiliation: Department of Biomechatronics, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon 440-746, South Korea. Electronic address: gkimbme@skku.edu.
authors.forename: Geun Hyung
authors.initials: GH
authors.lastname: Kim
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002478:Cells, Cultured; D003907:Dexamethasone / Q000008:administration & dosage*; D004353:Drug Evaluation, Preclinical; D065546:Drug Liberation; D004432:Ear, Middle / Q000187:drug effects / Q000473:pathology; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D005355:Fibrosis; D005938:Glucocorticoids / Q000008:administration & dosage*; D006168:Guinea Pigs; D006801:Humans; D020007:Implants, Experimental; D008297:Male; D009092:Mucous Membrane / Q000187:drug effects / Q000473:pathology; D012828:Silicones / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000464:Alginates; D000893:Anti-Inflammatory Agents; D001672:Biocompatible Materials; D005938:Glucocorticoids; D012828:Silicones; D003907:Dexamethasone
keywords: Alginate; Dexamethasone; Fibrosis; Silicone sheet