title: Advanced fibroblast proliferation inhibition for biocompatible coating by electrostatic layer-by-layer assemblies of heparin and chitosan derivatives.
abstract: Heparin and different chitosan derivatives were applied to produce stable electrostatic layer-by-layer assemblies and further used as coating technique to inhibit natural inflammatory response to implants. Heparin was assembled with chitosan and N-methylated chitosan derivatives, namely N,N-dimethyl chitosan (DMC) and N,N,N-trimethyl chitosan (TMC), by dipping method. DMC and TMC (chitosan derivatives) were synthesized and characterized before LbL assembly. Ellipsometry, quartz crystal microbalance (QCM-D), and contact angle were used to demonstrate the deposition of polyelectrolyte multilayers onto silicon wafers using polyelectrolyte solutions with different ionic strength. The biological properties of these films were evaluated by cell culture assays using NIH/3T3 fibroblast cells. LbL assemblies of Heparin and chitosan derivatives showed to be biocompatible, and at the same time they strongly hinder the proliferation speed of fibroblasts up to 40-fold factors. Therefore, the multilayers prepared from heparin and chitosan derivatives have good features to be used as an alternative coating treatment for biomedical implants with reduced body rejection properties.
authors.affiliation: Departamento de Química, Universidade Estadual de Maringá (UEM), Av. Colombo 5790, CEP 87020-90 Maringá, Paraná, Brazil; Institut Charles Sadron (CNRS-ULP), 23 rue du Loess, 67034 Strasbourg Cedex 2, France.
authors.forename: Heveline D M
authors.initials: HD
authors.lastname: Follmann
authors.affiliation: Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, CP 26077, CEP 05513-970 São Paulo, Brazil; Institut Charles Sadron (CNRS-ULP), 23 rue du Loess, 67034 Strasbourg Cedex 2, France.
authors.forename: Alliny F
authors.initials: AF
authors.lastname: Naves
authors.affiliation: Departamento de Química, Universidade Estadual de Maringá (UEM), Av. Colombo 5790, CEP 87020-90 Maringá, Paraná, Brazil; Universidade Tecnológica Federal do Paraná (UTFPR), Rua Marcílio Dias, CEP86812-460 Apucarana, Paraná, Brazil.
authors.forename: Alessandro F
authors.initials: AF
authors.lastname: Martins
authors.affiliation: Institut Charles Sadron (CNRS-ULP), 23 rue du Loess, 67034 Strasbourg Cedex 2, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Félix
authors.affiliation: Institut Charles Sadron (CNRS-ULP), 23 rue du Loess, 67034 Strasbourg Cedex 2, France.
authors.forename: Gero
authors.initials: G
authors.lastname: Decher
authors.affiliation: Departamento de Química, Universidade Estadual de Maringá (UEM), Av. Colombo 5790, CEP 87020-90 Maringá, Paraná, Brazil.
authors.forename: Edvani C
authors.initials: EC
authors.lastname: Muniz
authors.affiliation: Departamento de Química, Universidade Estadual de Maringá (UEM), Av. Colombo 5790, CEP 87020-90 Maringá, Paraná, Brazil. Electronic address: rafael_dqi@yahoo.com.br.
authors.forename: Rafael
authors.initials: R
authors.lastname: Silva
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects*; D006493:Heparin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D041681:NIH 3T3 Cells; D010316:Particle Size; D012965:Sodium Chloride / Q000494:pharmacology; D055672:Static Electricity; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D012965:Sodium Chloride; D006493:Heparin; D048271:Chitosan
keywords: Chitosan derivatives; Electrostatic layer-by-layer; Fibroblasts inhibition; Foreign body rejection; Heparin