title: The Effect of Chronic Methamphetamine Exposure on the Hippocampal and Olfactory Bulb Neuroproteomes of Rats.
abstract: Nowadays, drug abuse and addiction are serious public health problems in the USA. Methamphetamine (METH) is one of the most abused drugs and is known to cause brain damage after repeated exposure. In this paper, we conducted a neuroproteomic study to evaluate METH-induced brain protein dynamics, following a two-week chronic regimen of an escalating dose of METH exposure. Proteins were extracted from rat brain hippocampal and olfactory bulb tissues and subjected to liquid chromatography-mass spectrometry (LC-MS/MS) analysis. Both shotgun and targeted proteomic analysis were performed. Protein quantification was initially based on comparing the spectral counts between METH exposed animals and their control counterparts. Quantitative differences were further confirmed through multiple reaction monitoring (MRM) LC-MS/MS experiments. According to the quantitative results, the expression of 18 proteins (11 in the hippocampus and 7 in the olfactory bulb) underwent a significant alteration as a result of exposing rats to METH. 13 of these proteins were up-regulated after METH exposure while 5 were down-regulated. The altered proteins belonging to different structural and functional families were involved in processes such as cell death, inflammation, oxidation, and apoptosis.
authors.affiliation: Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, United States of America.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhu
authors.affiliation: Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, United States of America.
authors.forename: Tianjiao
authors.initials: T
authors.lastname: Yang
authors.affiliation: Department of Psychiatry, Center for Neuroproteomics and Biomarkers Research, University of Florida, Gainesville, FL, United States of America.
authors.forename: Firas
authors.initials: F
authors.lastname: Kobeissy
authors.affiliation: Faculty of Medicine, American University of Beirut Medical Center, Beirut, Lebanon.
authors.forename: Tarek H
authors.initials: TH
authors.lastname: Mouhieddine
authors.affiliation: Faculty of Medicine, American University of Beirut Medical Center, Beirut, Lebanon.
authors.forename: Mohamad
authors.initials: M
authors.lastname: Raad
authors.affiliation: Faculty of Medicine, Department of Biochemistry and Molecular Genetics, American University of Beirut Medical Center, Beirut, Lebanon.
authors.forename: Amaly
authors.initials: A
authors.lastname: Nokkari
authors.affiliation: Department of Psychiatry, Center for Neuroproteomics and Biomarkers Research, University of Florida, Gainesville, FL, United States of America.
authors.forename: Mark S
authors.initials: MS
authors.lastname: Gold
authors.affiliation: Department of Psychiatry, Center for Neuroproteomics and Biomarkers Research, University of Florida, Gainesville, FL, United States of America.
authors.forename: Kevin K
authors.initials: KK
authors.lastname: Wang
authors.affiliation: Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, United States of America.
authors.forename: Yehia
authors.initials: Y
authors.lastname: Mechref
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D000697:Central Nervous System Stimulants; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008694:Methamphetamine / Q000494:pharmacology*; D009830:Olfactory Bulb / Q000187:drug effects / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019966:Substance-Related Disorders; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000697:Central Nervous System Stimulants; D020543:Proteome; D008694:Methamphetamine