title: Influence of antibiotic adsorption on biocidal activities of silver nanoparticles.
abstract: Excessive use of antibiotics has posed two major challenges in public healthcare. One of them is associated with the development of multi-drug resistance while the other one is linked to side effects. In the present investigation, the authors report an innovative approach to tackle the challenges of multi-drug resistance and acute toxicity of antibiotics by using antibiotics adsorbed metal nanoparticles. Monodisperse silver nanoparticles (SNPs) have been synthesised by two-step process. In the first step, SNPs were prepared by chemical reduction of AgNO3 with oleylamine and in the second step, oleylamine capped SNPs were phase-transferred into an aqueous medium by ligand exchange. Antibiotics - tetracycline and kanamycin were further adsorbed on the surface of SNPs. Antibacterial activities of SNPs and antibiotic adsorbed SNPs have been investigated on gram-positive (Staphylococcus aureus, Bacillus megaterium, Bacillus subtilis), and gram-negative (Proteus vulgaris, Shigella sonnei, Pseudomonas fluorescens) bacterial strains. Synergistic effect of SNPs on antibacterial activities of tetracycline and kanamycin has been observed. Biocidal activity of tetracycline is improved by 0-346% when adsorbed on SNPs; while for kanamycin, the improvement is 110-289%. This synergistic effect of SNPs on biocidal activities of antibiotics may be helpful in reducing their effective dosages.
authors.affiliation: Laboratory of Nanomedicine, School of Physics and Materials Science, Thapar University, Patiala-147004, India.
authors.forename: Chandni
authors.initials: C
authors.lastname: Khurana
authors.affiliation: Department of Physics, M.K. Bhavnagar University, Bhavnagar-364022, India.
authors.forename: Anjana K
authors.initials: AK
authors.lastname: Vala
authors.affiliation: Laboratory of Nanomedicine, School of Physics and Materials Science, Thapar University, Patiala-147004, India.
authors.forename: Nidhi
authors.initials: N
authors.lastname: Andhariya
authors.affiliation: Laboratory of Nanomedicine, School of Physics and Materials Science, Thapar University, Patiala-147004, India.
authors.forename: O P
authors.initials: OP
authors.lastname: Pandey
authors.affiliation: Laboratory of Nanomedicine, School of Physics and Materials Science, Thapar University, Patiala-147004, India. bnchudasama@gmail.com.
authors.forename: Bhupendra
authors.initials: B
authors.lastname: Chudasama
mesh_terms: D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics* / Q000494:pharmacology; D001410:Bacillus megaterium / Q000187:drug effects / Q000254:growth & development; D001412:Bacillus subtilis / Q000187:drug effects / Q000254:growth & development; D057446:Hydrodynamics; D053768:Metal Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D010316:Particle Size; D011551:Pseudomonas fluorescens / Q000187:drug effects / Q000254:growth & development; D012834:Silver / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver