title: Ultrasensitive Lipopolysaccharides Detection Based on Doxorubicin Conjugated N-(Aminobutyl)-N-(ethylisoluminol) as Electrochemiluminescence Indicator and Self-Assembled Tetrahedron DNA Dendrimers as Nanocarriers.
abstract: The preparation of self-assembled DNA nanostructure with different sizes and shapes has been one of the most promising research areas in recent years, while the application of these DNA nanostructures in biosensors is far from fully developed. Here, we presented a novel carrier system to construct an electrochemiluminescence (ECL) aptasensor for ultrasensitive determination of lipopolysaccharides (LPS) on the basis of self-assembled tetrahedron DNA dendrimers. Doxorubicin (Dox), a well-known intercalator of double stranded DNA (dsDNA), was conjugated with the ECL luminophore of N-(aminobutyl)-N-(ethylisoluminol) (ABEI) to form a new type of ECL indicators (Dox-ABEI), which could noncovalently attach to dsDNA through intercalation. Based on this property, self-assembled tetrahedron DNA dendrimers were employed as an efficient nanocarrier to achieve a high loading efficiency for Dox-ABEI with significantly amplified ECL signal output. Streptavidin (SA) and biotin, a typical ligand-receptor pair, has been chosen to anchor the tetrahedron DNA dendrimers on the electrode surface. Moreover, by converting LPS content into DNA output, catalyzed hairpin assembly (CHA) target recycling signal amplification strategy was also adopted to enhance the sensitivity of the ECL aptasensor. With combining the loading power of the tetrahedron DNA dendrimers for ECL indicators, the inherent high sensitivity of ECL technique and target recycling for signal amplification, the proposed strategy showed a detection limit of 0.18 fg/mL for LPS.
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University , Chongqing 400715, People's Republic of China.|Chongqing Key Laboratory of Environmental Materials and Remediation Technologies, Chongqing University of Arts and Sciences , Chongqing 400715, People's Republic of China.
authors.forename: Shunbi
authors.initials: S
authors.lastname: Xie
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University , Chongqing 400715, People's Republic of China.
authors.forename: Yongwang
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University , Chongqing 400715, People's Republic of China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University , Chongqing 400715, People's Republic of China.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Chai
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University , Chongqing 400715, People's Republic of China.
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D001710:Biotin / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D050091:Dendrimers / Q000737:chemistry; D004317:Doxorubicin / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D052203:Ferrosoferric Oxide / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D007202:Indicators and Reagents; D057230:Limit of Detection; D008070:Lipopolysaccharides / Q000097:blood*; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000031:analogs & derivatives / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D019809:Streptavidin / Q000737:chemistry
chemical_list: D050091:Dendrimers; D004337:Drug Carriers; D007202:Indicators and Reagents; D008070:Lipopolysaccharides; D008165:Luminol; C031912:6-(N-(4-aminobutyl)-N-ethyl)amino-2,3-dihydrophthalazine-1,4-dione; D001710:Biotin; D006046:Gold; D004317:Doxorubicin; D004247:DNA; D019809:Streptavidin; D006861:Hydrogen Peroxide; D052203:Ferrosoferric Oxide