title: Optical Monitoring of Living Nerve Terminal Labeling in Hair Follicle Lanceolate Endings of the Ex Vivo Mouse Ear Skin.
abstract: A novel dissection and recording technique is described for optical monitoring staining and de-staining of lanceolate terminals surrounding hair follicles in the skin of the mouse pinna. The preparation is simple and relatively fast, reliably yielding extensive regions of multiple labeled units of living nerve terminals to study uptake and release of styryl pyridinium dyes extensively used in studies of vesicle recycling. Subdividing the preparations before labeling allows test vs. control comparisons in the same ear from a single individual. Helpful tips are given for improving the quality of the preparation, the labeling and the imaging parameters. This new system is suitable for assaying pharmacologically and mechanically-induced uptake and release of these vital dyes in lanceolate terminals in both wild-type and genetically modified animals. Examples of modulatory influences on labeling intensity are given.
authors.affiliation: School of Medical Sciences, University of Aberdeen; g.s.bewick@abdn.ac.uk.
authors.forename: Guy S
authors.initials: GS
authors.lastname: Bewick
authors.affiliation: School of Biological & Biomedical Sciences, University of Durham.
authors.forename: Robert W
authors.initials: RW
authors.lastname: Banks
mesh_terms: D000818:Animals; D004431:Ear, External; D005145:Face; D005456:Fluorescent Dyes / Q000378:metabolism; D018859:Hair Follicle / Q000294:innervation*; D008465:Mechanoreceptors / Q000378:metabolism*; D051379:Mice; D008856:Microscopy, Fluorescence; D009411:Nerve Endings / Q000378:metabolism*; D011726:Pyridinium Compounds / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D012867:Skin / Q000294:innervation*; D013194:Staining and Labeling / Q000379:methods
chemical_list: C073804:FM1 43; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds