title: Upregulation of lncRNA HOTAIR contributes to IL-1β-induced MMP overexpression and chondrocytes apoptosis in temporomandibular joint osteoarthritis.
abstract: Temporomandibular joint osteoarthritis (TMJ OA) is a common and heterogeneous disease that causes painful and progressive joint degeneration, which restricts daily activities, including talking and chewing. Long noncoding RNAs (lncRNAs) are an important class of genes involved in various physiological and pathological functions, including osteoarthritis (OA).The present study aimed to identify the lncRNAs that are important in TMJ OA and their potential functions. Here, we found that HOTAIR was significantly upregulated in the synovial fluid of TMJ OA patients compared with that of normal controls. Increased HOTAIR was similarly observed in the synovial fluid of TMJ OA rabbits as compared to control rabbits. Furthermore, in interleukin-1β (IL-1β)-induced TMJ OA in vitro model (primary rabbit condylar chondrocytes), the expressions of matrix metalloproteinase (MMP)-1, MMP3, MMP9 and HOTAIR were all dramatically increased. Most importantly, knockdown of HOTAIR in IL-1β-induced TMJ OA in vitro model could not only reverse the IL-1β-stimulated expressions of MMP1, MMP3 and MMP9, but also significantly decrease the apoptosis rate induced by IL-1β in primary rabbit condylar chondrocytes. Our data provides new insight into the mechanisms of chondrocytes destruction in TMJ OA.
authors.affiliation: Department of Stomatology, Yantai Yuhuangding Hospital, China.
authors.forename: Chunping
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Stomatology, Dental Hospital of Yantai City, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Medical Service, Yantai Yuhuangding Hospital, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Jiang
authors.affiliation: Department of Radiology, Yantai Yuhuangding Hospital, China.
authors.forename: Yongbin
authors.initials: Y
authors.lastname: Lv
authors.affiliation: Department of Ophthalmology, Yantai Yuhuangding Hospital, China.
authors.forename: Changxia
authors.initials: C
authors.lastname: Dong
authors.affiliation: Department of Ophthalmology, Yantai Yuhuangding Hospital, China.
authors.forename: Xiuyu
authors.initials: X
authors.lastname: Dai
authors.affiliation: Department of Ophthalmology, Yantai Yuhuangding Hospital, China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Tan
authors.affiliation: Department of Stomatology, Yantai Yuhuangding Hospital, China. Electronic address: sdytwzl@163.com.
authors.forename: Zhenlin
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000328:Adult; D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology*; D020782:Matrix Metalloproteinases / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D010003:Osteoarthritis / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D062085:RNA, Long Noncoding / Q000378:metabolism*; D011817:Rabbits; D013582:Synovial Fluid / Q000378:metabolism; D013705:Temporomandibular Joint Disorders / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015854:Up-Regulation
chemical_list: C561566:HOTAIR long untranslated RNA, human; D053583:Interleukin-1beta; D062085:RNA, Long Noncoding; D020782:Matrix Metalloproteinases
keywords: Apoptosis; HOTAIR; IL-1β; Long non-coding RNA; Temporomandibular joint osteoarthritis