title: Production of Japanese Encephalitis Virus-Like Particles Using Insect Cell Expression Systems.
abstract: Virus-like particles (VLPs) can be produced via the expression of virus surface proteins that self-assemble into particulate structures in recombinant protein expression systems. Expression of the DNA fragment encoding the Japanese encephalitis (JE) virus prM signal peptide, the precursor (prM) of the viral membrane protein (M), and the envelope glycoprotein (E) allows the production of a secretory form of VLPs. Expression systems that use lepidopteran insect cells, such as the baculovirus-insect cell system and stably transformed insect cells, can be used for the efficient production of JE VLPs. This chapter describes the production of JE VLPs from stably transformed lepidopteran insect cells.
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan. yamaji@kobe-u.ac.jp.
authors.forename: Hideki
authors.initials: H
authors.lastname: Yamaji
authors.affiliation: BIKEN Endowed Department of Dengue Vaccine Development, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Ratchathewi, Bangkok, 10400, Thailand.|BIKEN Endowed Department of Dengue Vaccine Development, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka, 565-0871, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Konishi
mesh_terms: D000818:Animals; D004664:Encephalitis Virus, Japanese / Q000235:genetics*; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D061987:Sf9 Cells; D018411:Spodoptera; D014170:Transformation, Genetic; D058425:Vaccines, Virus-Like Particle / Q000235:genetics*
chemical_list: D058425:Vaccines, Virus-Like Particle
keywords: Baculovirus–insect cell system; Flavivirus; High Five cells; Insect cells; Japanese encephalitis virus; Recombinant protein production; Stably transformed cells; Virus-like particles