title: Insertion sequence ISRP10 inactivation of the oprD gene in imipenem-resistant Pseudomonas aeruginosa clinical isolates.
abstract: Carbapenem resistance mechanisms were investigated in 32 imipenem-resistant Pseudomonas aeruginosa clinical isolates recovered from hospitalised children. Sequence analysis revealed that 31 of the isolates had an insertion sequence element ISRP10 disrupting the porin gene oprD, demonstrating that ISRP10 inactivation of oprD conferred imipenem resistance in the majority of the isolates. Multilocus sequence typing (MLST) was used to discriminate the isolates. In total, 11 sequence types (STs) were identified including 3 novel STs, and 68.3% (28/41) of the tested strains were characterised as clone ST253. In combination with random amplified polymorphic DNA (RAPD) analysis, the imipenem-resistant isolates displayed a relatively high degree of genetic variability and were unlikely associated with nosocomial infections.
authors.affiliation: Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
authors.forename: Qinghui
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
authors.forename: Zhaofen
authors.initials: Z
authors.lastname: Ba
authors.affiliation: Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
authors.forename: Guoying
authors.initials: G
authors.lastname: Wu
authors.affiliation: Tianjin Children's Hospital, Tianjin 300074, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Tianjin Children's Hospital, Tianjin 300074, China.
authors.forename: Shuxiang
authors.initials: S
authors.lastname: Lin
authors.affiliation: Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China. Electronic address: hongjiangyang@tust.edu.cn.
authors.forename: Hongjiang
authors.initials: H
authors.lastname: Yang
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002648:Child; D002675:Child, Preschool; D004251:DNA Transposable Elements; D020868:Gene Silencing; D005838:Genotype; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D058885:Multilocus Sequence Typing; D016254:Mutagenesis, Insertional; D018272:Porins / Q000235:genetics*; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D019105:Random Amplified Polymorphic DNA Technique; D018440:beta-Lactam Resistance
chemical_list: D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D018272:Porins; C077157:OprD protein, Pseudomonas aeruginosa; D015378:Imipenem
keywords: ISRP10; Imipenem resistance; MLST; Pseudomonas aeruginosa; RAPD; oprD gene