title: Serca2a and Na(+)/Ca(2+) exchanger are involved in left ventricular function following cardiac remodelling of female rats treated with anabolic androgenic steroid.
abstract: Anabolic-androgenic steroids are misused, including by women, but little is known about the cardiovascular effects of these drugs on women.
  
  
  
  To evaluated the effects of nandrolone decanoate (ND) and resistive physical exercise on cardiac contractility in young female rats.
  
  
  
  Female Wistar rats were separated into 4 groups: C (untrained animals); E (animals were submitted to resistance exercise by jumping in water 5 times per week); ND (animals were treated with ND, 20mg/kg/week for 4weeks); and NDE (trained and treated). The haemodynamic parameters (+dP/dtmax, -dP/dtmin and Tau) were assessed in the left ventricle. The heart was collected for histological analyses and collagen deposition. The gastrocnemius muscle was weighed, and hypertrophy was assessed by the ratio of their weights to gastrocnemius/tibia length. The expression of calcium handling proteins was measured by western blot analysis.
  
  
  
  ND treatment and physical exercise increased cardiac contractility and relaxation. In addition, ND promoted increases in phospholamban phosphorylated (p-PLB) and isoforms of sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (SERCA2a) expression, while resistance exercise increased the phosphorylation of PLB and expression of Na(+)/Ca(2+) exchangers (NCX). Cardiac hypertrophy and collagen deposition were observed after ND treatment.
  
  
  
  Regulatory components of cytosolic calcium, such as SERCA2a and p-PLB, play important roles in modulating the contractility and relaxation effects of ND in females.
authors.affiliation: Department of Physiological Sciences, Federal University of Espirito Santo, Vitória, Espirito Santo, Brazil.
authors.forename: Andrews Marques do
authors.initials: AM
authors.lastname: Nascimento
authors.affiliation: Department of Physiological Sciences, Federal University of Espirito Santo, Vitória, Espirito Santo, Brazil.
authors.forename: Ewelyne Miranda de
authors.initials: EM
authors.lastname: Lima
authors.affiliation: Department of Physiological Sciences, Federal University of Espirito Santo, Vitória, Espirito Santo, Brazil.
authors.forename: Girlandia Alexandre
authors.initials: GA
authors.lastname: Brasil
authors.affiliation: Department of Physiological Sciences, Federal University of Espirito Santo, Vitória, Espirito Santo, Brazil.
authors.forename: Izabela Facco
authors.initials: IF
authors.lastname: Caliman
authors.affiliation: Department of Physiology and Biophysic, Federal University of Minas Gerais, Minas Gerais, Brazil.
authors.forename: Josiane Fernandes da
authors.initials: JF
authors.lastname: Silva
authors.affiliation: Department of Physiology and Biophysic, Federal University of Minas Gerais, Minas Gerais, Brazil.
authors.forename: Virgínia Soares
authors.initials: VS
authors.lastname: Lemos
authors.affiliation: Department of Pharmacy, University Vila Velha, Vila Velha, Espirito Santo, Brazil.
authors.forename: Tadeu Uggere de
authors.initials: TU
authors.lastname: Andrade
authors.affiliation: Department of Physiological Sciences, Federal University of Espirito Santo, Vitória, Espirito Santo, Brazil. Electronic address: nazarebissoli@gmail.com.
authors.forename: Nazaré Souza
authors.initials: NS
authors.lastname: Bissoli
mesh_terms: D045930:Anabolic Agents / Q000494:pharmacology*; D000818:Animals; D062186:Arterial Pressure / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism; D006332:Cardiomegaly / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D003094:Collagen / Q000378:metabolism; D005260:Female; D006339:Heart Rate / Q000187:drug effects; D018482:Muscle, Skeletal / Q000187:drug effects; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D009277:Nandrolone / Q000031:analogs & derivatives* / Q000494:pharmacology; D000077603:Nandrolone Decanoate; D017208:Rats, Wistar; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000378:metabolism*; D019831:Sodium-Calcium Exchanger / Q000378:metabolism*; D016277:Ventricular Function, Left / Q000502:physiology*; D020257:Ventricular Remodeling / Q000502:physiology*; D000099211:Phospholamban
chemical_list: D045930:Anabolic Agents; D002118:Calcium; D002135:Calcium-Binding Proteins; D003094:Collagen; D009277:Nandrolone; D000077603:Nandrolone Decanoate; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D019831:Sodium-Calcium Exchanger; D000099211:Phospholamban
keywords: Cardiac function; Left ventricular hypertrophy; NCX; Nandrolone decanoate; Resistive exercise; SERCA2a