title: Effects of siRNA-Mediated Knockdown of HDAC1 on the Biological Behavior of Esophageal Carcinoma Cell Lines.
abstract: BACKGROUND HDAC1 has been shown to be closely associated with the occurrence of tumors. We aimed to investigate the effects of siRNA-mediated HDAC1 knockdown on the biological behavior of esophageal carcinoma cell lines. MATERIAL AND METHODS HDAC1 expression in esophageal cancer cell lines TE-1, Eca109, and EC9706 was compared by Western blot analysis. These cells were transfected with siRNA-HDAC1 and cell proliferation was evaluated by MTT assay to select the optimum cell line for subsequent experiments. The effects of siRNA-HDAC1 on the migration and invasion of the selected cell line were assessed by transwell assay. The expression of cell cycle-related proteins cyclinD1, p21 and p27, and epithelial-mesenchymal transition (EMT)-related protein zonula occludens-1 (ZO-1), E-cadherin and vimentin was determined by Western blot analysis. RESULTS HDAC1 expression in TE-1, Eca109 and EC9706 cells was significantly higher compared with normal esophageal cell line HEEC (P<0.01). MTT assay, Western blot and RT-PCR analyses demonstrated that the inhibitory effects of siRNA on HDAC1 expression and cell viability in TE-1 cells were the highest among all cell lines, which was therefore used in subsequent experiments. After TE-1 cells were transfected with siRNA-HDAC1, their migration and invasion were significantly lower compared with the controls (P<0.01). CyclinD1 and vimentin expression was significantly lower compared with the controls (P<0.01), whereas the expression of p21, p27, ZO-1 and E-cadherin was significantly higher (P<0.01). CONCLUSIONS The siRNA-mediated HDAC1 knockdown significantly inhibited the proliferation, migration and invasion of TE-1 cells probably by regulating the expression of cell cycle- and EMT-related proteins.
authors.affiliation: Department of Oncology, Dongying People's Hospital of Shandong Province, Dongying, Shandong, China (mainland).
authors.forename: Xing
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Oncology, Dongying People's Hospital of Shandong Province, Dongying, Shandong, China (mainland).
authors.forename: Haisheng
authors.initials: H
authors.lastname: Guo
authors.affiliation: Department of Oncology, Dongying People's Hospital of Shandong Province, Dongying, Shandong, China (mainland).
authors.forename: Weixin
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Oncology, Dongying People's Hospital of Shandong Province, Dongying, Shandong, China (mainland).
authors.forename: Chunmei
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Digestive, Dongying People's Hospital of Shandong Province, Dongying, Shandong, China (mainland).
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Pathology, Dongying People's Hospital of Shandong Province, Dongying, Shandong, China (mainland).
authors.forename: Dongguan
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Oncology, Dongying People's Hospital of Shandong Province, Dongying, Shandong, China (mainland).
authors.forename: Xunguo
authors.initials: X
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000502:physiology; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D002453:Cell Cycle / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D015536:Down-Regulation; D004938:Esophageal Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D056284:Histone Deacetylase 1 / Q000172:deficiency* / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics; D014162:Transfection
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; C110550:HDAC1 protein, human; D056284:Histone Deacetylase 1