title: Aflatoxin B1 in sesame seeds and sesame products from the Greek market.
abstract: Aflatoxin B1 (AFB1) is considered as the most potent liver carcinogen for humans. A method for determination in sesame seeds was developed. AFB1 was extracted by methanol-water, cleaned by immunoaffinity columns and determined by high-performance liquid chromatography with fluorescence detection. The recovery factor and the limit of detection (LOD) of AFB1 in sesame seeds were 111.5% and 0.02 ng g(-1), respectively. Thirty samples of sesame products were examined for the presence of AFB1. After analysis, 77.6% of samples were found to be contaminated. Eight samples exceeded the European Union (EU) limit (2 µg AFB1 kg(-1)). In 15 samples, AFB1 was below the EU limit. Seven samples remained below the LOD. The most contaminated (14.49 ng AFB1 g(-1)) sample was unpeeled packaged sesame seeds. In all samples, aflatoxigenic Aspergilli fungi as well as the risk for AFB1 presence in sesame seed was investigated.
authors.affiliation: a Department of Chemistry, Laboratory of Food Chemistry , National and Kapodistrian University of Athens , Athens , Greece.
authors.forename: Eleni
authors.initials: E
authors.lastname: Kollia
authors.affiliation: a Department of Chemistry, Laboratory of Food Chemistry , National and Kapodistrian University of Athens , Athens , Greece.
authors.forename: Kyriakos
authors.initials: K
authors.lastname: Tsourouflis
authors.affiliation: a Department of Chemistry, Laboratory of Food Chemistry , National and Kapodistrian University of Athens , Athens , Greece.
authors.forename: Panagiota
authors.initials: P
authors.lastname: Markaki
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis* / Q000633:toxicity; D053000:Analytic Sample Preparation Methods; D001230:Aspergillus / Q000254:growth & development / Q000302:isolation & purification; D002274:Carcinogens, Environmental / Q000032:analysis* / Q000633:toxicity; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D005062:European Union; D005506:Food Contamination / Q000517:prevention & control; D005511:Food Handling; D005513:Food Inspection / Q000379:methods; D006115:Greece; D006801:Humans; D038622:Internationality; D057230:Limit of Detection; D019055:No-Observed-Adverse-Effect Level; D015203:Reproducibility of Results; D018570:Risk Assessment; D012639:Seeds / Q000009:adverse effects / Q000737:chemistry* / Q000382:microbiology; D012715:Sesame Oil / Q000009:adverse effects / Q000737:chemistry* / Q000592:standards; D031685:Sesamum / Q000009:adverse effects / Q000737:chemistry* / Q000382:microbiology; D013050:Spectrometry, Fluorescence
chemical_list: D002274:Carcinogens, Environmental; D012715:Sesame Oil; D016604:Aflatoxin B1
keywords: Aflatoxin B1; HPLC; aflatoxigenic fungi; sesame products