title: siRNA targeting YAP gene inhibits gastric carcinoma growth and tumor metastasis in SCID mice.
abstract: Yes-associated protein (YAP) is constitutively activated in numerous types of cancer, including gastric carcinoma. The aim of the present study was to investigate the effects of YAP silencing on proliferation, apoptosis, metastasis and angiogenesis in a gastric orthotopic implantation cancer model of severe combined immunodeficiency mice. Small-hairpin RNA (shRNA) targeting the YAP gene was employed to inhibit YAP expression. SGC7901 cells transfected with YAP shRNA demonstrated significantly decreased gastric cancer growth and metastasis in the orthotopic implantation mouse model. Silencing of YAP additionally promoted tumor cell apoptosis, and inhibited tumor cell proliferation and angiogenesis. Notably, YAP shRNA also downregulated the expression of TEA domain family member 1, cyclinD1, vascular endothelial growth factor and fibroblast growth factor-2. The results of the present study suggested that YAP may have a significant role in the proliferation, metastasis and angiogenesis of gastric cancer. RNA interference-mediated silencing of YAP may provide an opportunity to develop a novel treatment strategy for gastric cancer.
authors.affiliation: Department of Gastroenterology, Sichuan Provincial People's Hospital, Chengdu, Sichuan 610072, P.R. China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Gastroenterology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China.
authors.forename: Jin-Shui
authors.initials: JS
authors.lastname: Zhu
authors.affiliation: Department of Gastroenterology, Sichuan Provincial People's Hospital, Chengdu, Sichuan 610072, P.R. China.
authors.forename: Cai-Ping
authors.initials: CP
authors.lastname: Gao
authors.affiliation: Department of Gastroenterology, Sichuan Provincial People's Hospital, Chengdu, Sichuan 610072, P.R. China.
authors.forename: Liang-Ping
authors.initials: LP
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, Sichuan Provincial People's Hospital, Chengdu, Sichuan 610072, P.R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Department of Gastroenterology, Sichuan Provincial People's Hospital, Chengdu, Sichuan 610072, P.R. China.
authors.forename: Han
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Sichuan Provincial People's Hospital, Chengdu, Sichuan 610072, P.R. China.
authors.forename: Xiao-Gang
authors.initials: XG
authors.lastname: Liu
keywords: RNA interference; gastric carcinoma; orthotopic implantation mouse model; yes-associated protein gene