title: Metabolic product response profiles of Cherax quadricarinatus towards white spot syndrome virus infection.
abstract: White spot syndrome virus (WSSV) is one of the most devastating viral pathogens in both shrimp and crayfish farms, which often causes disease outbreak and leads to massive moralities with significant economic losses of aquaculture. However, limited research has been carried out on the intrinsic mechanisms toward WSSV challenge at the metabolic level. To gain comprehensive insight into metabolic responses induced by WSSV, we applied an NMR approach to investigate metabolic changes of crayfish gill and hepatopancreas infected by WSSV for 1, 6 and 12 h. In gill, an enhanced energy metabolism was observed in WSSV-challenged crayfish samples at 1 h, as marked by increased glucose, alanine, methionine, glutamate and uracil. Afterwards, energy metabolism, lipid metabolism as well as osmoregulation were markedly increased at 6 hpi, as shown by elevated glucose, alanine, methionine, fumarate, tyrosine, tryptophan, histidine, phosphorylcholine, betaine and uracil, whereas no obvious metabolites change was detected at 12 hpi. As for hepatopancreas, disturbed lipid metabolism and induced osmotic regulation was found at 6 hpi based on the metabolic biomarkers such as branched chain amino acids, threonine, alanine, methionine, glutamate, glutamine, tyrosine, phenylalanine, lactate and lipid. However, no obvious metabolic change was shown in hepatopancreas at both 1 hpi and 12 hpi. Taken together, our present results provided essential metabolic information about host-pathogen interactions in crayfish, which shed new light on our understanding of WSSV infection at metabolic level.
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, PR China; State Key Laboratory of Marine Environmental Science, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, Xiamen University, Xiamen 361102, Fujian, PR China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Fan
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, PR China.
authors.forename: Yangfang
authors.initials: Y
authors.lastname: Ye
authors.affiliation: State Key Laboratory of Marine Environmental Science, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, Xiamen University, Xiamen 361102, Fujian, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Chen
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, PR China.
authors.forename: Yina
authors.initials: Y
authors.lastname: Shao
authors.affiliation: State Key Laboratory of Marine Environmental Science, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, Xiamen University, Xiamen 361102, Fujian, PR China.
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Xie
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, PR China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Marine Environmental Science, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, Xiamen University, Xiamen 361102, Fujian, PR China. Electronic address: haipengliu@xmu.edu.cn.
authors.forename: Hai-Peng
authors.initials: HP
authors.lastname: Liu
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, PR China. Electronic address: lichenghua@nbu.edu.cn.
authors.forename: Chenghua
authors.initials: C
authors.lastname: Li
mesh_terms: D000818:Animals; D017756:Aquaculture; D003400:Astacoidea / Q000276:immunology*; D004266:DNA Virus Infections / Q000276:immunology*; D004734:Energy Metabolism; D005880:Gills / Q000378:metabolism*; D043143:Hepatopancreas / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D050356:Lipid Metabolism; D055442:Metabolome / Q000276:immunology*; D019906:Nuclear Magnetic Resonance, Biomolecular; D046848:White spot syndrome virus 1 / Q000276:immunology*
keywords: Cherax quadricarinatus; Gill; Hepatopancreas; Metabolomics; WSSV