title: Single Cell Protein Production by Saccharomyces cerevisiae Using an Optimized Culture Medium Composition in a Batch Submerged Bioprocess.
abstract: Saccharomyces cerevisiae PTCC5269 growth was evaluated to specify an optimum culture medium to reach the highest protein production. Experiment design was conducted using a fraction of the full factorial methodology, and signal to noise ratio was used for results analysis. Maximum cell of 8.84 log (CFU/mL) was resulted using optimized culture composed of 0.3, 0.15, 1, and 50 g L(-1) of ammonium sulfate, iron sulfate, glycine, and glucose, respectively at 300 rpm and 35 °C. Glycine concentration (39.32 % contribution) and glucose concentration (36.15 % contribution) were determined as the most effective factors on the biomass production, while Saccharomyces cerevisiae growth had showed the least dependence on ammonium sulfate (5.2 % contribution) and iron sulfate (19.28 % contribution). The most interaction was diagnosed between ammonium sulfate and iron sulfate concentrations with interaction severity index of 50.71 %, while the less one recorded for glycine and glucose concentration was equal to 8.12 %. An acceptable consistency of 84.26 % was obtained between optimum theoretical cell numbers determined by software of 8.91 log (CFU/mL), and experimentally measured one at optimal condition confirms the suitability of the applied method. High protein content of 44.6 % using optimum culture suggests that Saccharomyces cerevisiae is a good commercial case for single cell protein production.
authors.affiliation: Department of Chemical Engineering, Shahrood Branch, Islamic Azad University, Shahrood, Iran.
authors.forename: Mehrnoosh
authors.initials: M
authors.lastname: Hezarjaribi
authors.affiliation: Department of Chemical Engineering, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran. f.ardestani@qaemshahriau.ac.ir.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Ardestani
authors.affiliation: Department of Chemical Engineering, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Hamid Reza
authors.initials: HR
authors.lastname: Ghorbani
mesh_terms: D061249:Batch Cell Culture Techniques / Q000379:methods*; D018533:Biomass; D049109:Cell Proliferation / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology*; D004044:Dietary Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*
chemical_list: D003470:Culture Media; D004044:Dietary Proteins; C012551:single cell proteins
keywords: Culture medium composition; Optimization; Saccharomyces cerevisiae; Single cell protein; Taguchi approach