title: In vivo and in vitro phagocytosis of Leishmania (Leishmania) amazonensis promastigotes by B-1 cells.
abstract: Leishmaniasis is caused by Leishmania parasites that infect several cell types. The promastigote stage of Leishmania is internalized by phagocytic cells and transformed into the obligate intracellular amastigote form. B-1 cells are a subpopulation of B cells that are able to differentiate in vitro and in vivo into mononuclear phagocyte-like cells with phagocytic properties. B-1 cells use several receptors for phagocytosis, such as the mannose receptor and third complement receptor. Leishmania binds to the same receptors on macrophages. In this study, we demonstrated that phagocytes derived from B-1 cells (B-1 CDP) were able to internalize promastigotes of L. (L.) amazonensis in vitro. The internalized promastigotes differentiated into amastigotes. Our results showed that the phagocytic index was higher in B-1 CDP compared to peritoneal macrophages and bone marrow-derived macrophages. The in vivo phagocytic ability of B-1 cells was also demonstrated. Parasites were detected inside purified B-1 cells after intraperitoneal infection with L. (L.) amazonensis promastigotes. Intraperitoneal stimulation with the parasites led to an increase in both IL-10 and TNF-α. These results highlight the importance of studying B-1 CDP cells as phagocytic cells that can participate and contribute to immunity to parasites.
authors.affiliation: Departamento de Ciências Biológicas, Universidade Federal de São Paulo - Campus Diadema, Diadema, São Paulo, Brazil.
authors.forename: M M
authors.initials: MM
authors.lastname: Geraldo
authors.affiliation: Departamento de Ciências Biológicas, Universidade Federal de São Paulo - Campus Diadema, Diadema, São Paulo, Brazil.
authors.forename: C R
authors.initials: CR
authors.lastname: Costa
authors.affiliation: Departamento de Ciências Biológicas, Universidade Federal de São Paulo - Campus Diadema, Diadema, São Paulo, Brazil.
authors.forename: F M C
authors.initials: FM
authors.lastname: Barbosa
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, São Paulo, São Paulo, Brazil.
authors.forename: B C
authors.initials: BC
authors.lastname: Vivanco
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, São Paulo, São Paulo, Brazil.
authors.forename: W F K M
authors.initials: WF
authors.lastname: Gonzaga
authors.affiliation: Centro Universitário São Camilo, São Paulo, São Paulo, Brazil.
authors.forename: R R
authors.initials: RR
authors.lastname: Novaes E Brito
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, São Paulo, São Paulo, Brazil.
authors.forename: A F
authors.initials: AF
authors.lastname: Popi
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, São Paulo, São Paulo, Brazil.|Universidade Federal de São Paulo - Campus Diadema, Diadema, São Paulo, Brazil.
authors.forename: J D
authors.initials: JD
authors.lastname: Lopes
authors.affiliation: Departamento de Ciências Biológicas, Universidade Federal de São Paulo - Campus Diadema, Diadema, São Paulo, Brazil.
authors.forename: P
authors.initials: P
authors.lastname: Xander
mesh_terms: D000818:Animals; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D002478:Cells, Cultured; D006801:Humans; D016753:Interleukin-10 / Q000276:immunology; D007891:Leishmania / Q000276:immunology* / Q000502:physiology; D007896:Leishmaniasis / Q000276:immunology* / Q000469:parasitology; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010586:Phagocytes / Q000276:immunology; D010587:Phagocytosis
chemical_list: D016753:Interleukin-10
keywords: B-1 cells; Leishmania (Leishmania) amazonensis; phagocytosis