title: Intensity interrogation near cutoff resonance for label-free cellular profiling.
abstract: We report a method enabling intensity-based readout for label-free cellular assays, and realize a reader device with the same footprint as a microtiter plate. For unambiguous resonance intensity measurements in resonance waveguide grating (RWG) sensors, we propose to apply resonances near the substrate cutoff wavelength. This method was validated in bulk refractive index, surface bilayer and G protein-coupled receptor (GPCR) experiments. The significantly reduced size of the reader device opens new opportunities for easy integration into incubators or liquid handling systems.
authors.affiliation: Byosens GmbH, 20357 Hamburg, Germany.
authors.forename: Yousef
authors.initials: Y
authors.lastname: Nazirizadeh
authors.affiliation: Byosens GmbH, 20357 Hamburg, Germany.
authors.forename: Volker
authors.initials: V
authors.lastname: Behrends
authors.affiliation: Nanobiosensorics Group, Hungarian Academy of Sciences, Research Centre for Energy Research, Konkoly-Thege, 1120 Budapest, Hungary.
authors.forename: Aurél
authors.initials: A
authors.lastname: Prósz
authors.affiliation: Nanobiosensorics Group, Hungarian Academy of Sciences, Research Centre for Energy Research, Konkoly-Thege, 1120 Budapest, Hungary.
authors.forename: Norbert
authors.initials: N
authors.lastname: Orgovan
authors.affiliation: Nanobiosensorics Group, Hungarian Academy of Sciences, Research Centre for Energy Research, Konkoly-Thege, 1120 Budapest, Hungary.
authors.forename: Robert
authors.initials: R
authors.lastname: Horvath
authors.affiliation: Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, New York 14831, USA.
authors.forename: Ann M
authors.initials: AM
authors.lastname: Ferrie
authors.affiliation: Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, New York 14831, USA.
authors.forename: Ye
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Institute for Materials Science, Christian-Albrechts-Universität zu Kiel, 24143 Kiel, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Selhuber-Unkel
authors.affiliation: Institute of Electrical Engineering and Information Technology, Christian-Albrechts-Universität zu Kiel, 24143 Kiel, Germany.
authors.forename: Martina
authors.initials: M
authors.lastname: Gerken
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002460:Cell Line; D045744:Cell Line, Tumor; D005347:Fibroblasts / Q000166:cytology; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008051:Lipid Bilayers / Q000378:metabolism; D061848:Optical Imaging / Q000295:instrumentation / Q000379:methods*; D051381:Rats; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism
chemical_list: D008051:Lipid Bilayers; D043562:Receptors, G-Protein-Coupled