title: Targeted Gene Expression in Zebrafish Exposed to Chlorpyrifos-Oxon Confirms Phenotype-Specific Mechanisms Leading to Adverse Outcomes.
abstract: Zebrafish models for mild, moderate, and severe acute organophosphorus poisoning were previously developed by exposing zebrafish larvae to chlopyrifos-oxon. The phenotype of these models was characterized at several levels of biological organization. Oxidative stress and mitochondrial dysfunction were found to be involved in the development of the more severe phenotype. Here we used targeted gene expression to understand the dose-responsiveness of those two pathways and their involvement on generating the different zebrafish models. As the severe phenotype is irreversible after only 3 h of exposure, we also analyzed the response of the oxidative stress pathway at 3 and 24 h. Some of the genes related to oxidative stress were already differentially expressed at 3 h. There was an increase in differentially expressed genes related to both oxidative stress and mitochondrial function from the more mild to the more severe phenotype, suggesting the involvement of these mechanisms in increasing phenotype severity. Temporal data suggest that peroxynitrite leading to lipid peroxidation might be involved in phenotype transition and irreversibility.
authors.affiliation: US Army Engineer Research and Development Center, 3909 Halls Ferry Rd, Vicksburg, MS, 39180, USA. natalia.g.vinas@erdc.dren.mil.|Institute for Genomics, Biocomputing & Biotechnology, Mississippi State University, 2 Research Blvd, Starkville, MS, 39759, USA. natalia.g.vinas@erdc.dren.mil.
authors.forename: Natàlia
authors.initials: N
authors.lastname: Garcia-Reyero
authors.affiliation: US Army Engineer Research and Development Center, 3909 Halls Ferry Rd, Vicksburg, MS, 39180, USA.
authors.forename: Lynn
authors.initials: L
authors.lastname: Escalon
authors.affiliation: CIC-CSIC, Jordi Girona 18, 08034, Barcelona, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Prats
authors.affiliation: Department of Biology and CESAM, University of Aveiro, Aveiro, Portugal.|IDAEA-CSIC, Jordi Girona 18, 08034, Barcelona, Spain.
authors.forename: Melissa
authors.initials: M
authors.lastname: Faria
authors.affiliation: Department of Biology and CESAM, University of Aveiro, Aveiro, Portugal.
authors.forename: Amadeu M V M
authors.initials: AM
authors.lastname: Soares
authors.affiliation: IDAEA-CSIC, Jordi Girona 18, 08034, Barcelona, Spain.
authors.forename: Demetrio
authors.initials: D
authors.lastname: Raldúa
mesh_terms: D000818:Animals; D004390:Chlorpyrifos / Q000031:analogs & derivatives* / Q000633:toxicity; D015870:Gene Expression / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010641:Phenotype; D015027:Zebrafish / Q000378:metabolism
chemical_list: C009618:O,O-diethyl O-3,5,6-trichloro-2-pyridyl phosphate; D004390:Chlorpyrifos
keywords: Chlorpyrifos-oxon; Gene expression; Mitochondria; Oxidative stress; Zebrafish