title: Development of a blocking ELISA for detection of Mycoplasma hyopneumoniae infection based on a monoclonal antibody against protein P65.
abstract: Mycoplasma hyopneumoniae causes porcine enzootic pneumonia, an economically important disease of swine. A more sensitive and reliable method for detection of serum antibodies is needed for epidemiological investigations and to evaluate the effect of immunization. We expressed the M. hyopneumoniae protein P65 in Escherichia coli and produced a monoclonal antibody (mAb) that bound specifically to recombinant P65. Using this mAb, a blocking enzyme linked immunosorbent assay (ELISA) was developed. The blocking ELISA had similar specificity to and sensitivity with the commercial ELISA produced by IDEXX. Thus, this blocking ELISA is a useful test for serological confirmation of M. hyopneumoniae infection.
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences. Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture. National Center for Engineering Research of Veterinary Bio-Products, Nanjing 210014, China.
authors.forename: Maojun
authors.initials: M
authors.lastname: Liu
authors.forename: Gaimei
authors.initials: G
authors.lastname: DU
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yuzi
authors.initials: Y
authors.lastname: Wu
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Yun
authors.initials: Y
authors.lastname: Bai
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Feng
authors.forename: Qiyan
authors.initials: Q
authors.lastname: Xiong
authors.forename: Fangfang
authors.initials: F
authors.lastname: Bai
authors.forename: Glenn F
authors.initials: GF
authors.lastname: Browning
authors.forename: Guoqing
authors.initials: G
authors.lastname: Shao
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D015153:Blotting, Western / Q000662:veterinary; D003429:Cross Reactions / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D045705:Mycoplasma hyopneumoniae / Q000276:immunology*; D045729:Pneumonia of Swine, Mycoplasmal / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D012680:Sensitivity and Specificity; D013552:Swine
chemical_list: D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins