title: Electro-Acupuncture Promotes Endogenous Multipotential Mesenchymal Stem Cell Mobilization into the Peripheral Blood.
abstract: OBJECTIVE
  Mobilization of endogenous stem cells is an appealing strategy for cell therapy However, there is little evidence for reproducible, effective methods of mesenchymal stem cell (MSC) mobilization. In the present study, we investigated the mobilizing effect of electro-acupuncture (EA) on endogenous MSCs.
  
  
  METHODS
  Normal adult rats were randomly divided into six groups, namely, EA for 14 days (EA14d), sham EA14d, EA21d, sham EA21d and matched control groups. MSC mobilization efficiency was determined by colony-forming unit fibroblast (CFU-F) assays. Mobilized peripheral blood (PB)-derived MSCs were identified by immunophenotype and multi-lineage differentiation potential.
  
  
  RESULTS
  CFU-F frequency was significantly increased in the PB of EA14d rats compared with the sham EA and control groups. Moreover, the number of CFU-Fs was increased further in the EA21d group. MSCs derived from EA-mobilized PB were positive for CD90 and CD44, but negative for CD45. Additionally, these cells could differentiate into adipocytes, osteoblasts, chondrocytes and neural-like cells in vitro. Finally, stromal cell-derived factor-1α (SDF-1α) was increased in the PB of rats subjected to EA, and the migration of MSCs was improved in response to SDF-1α.
  
  
  CONCLUSIONS
  MSCs with multi-lineage differentiation potential can be mobilized by EA. Our data provide a promising strategy for MSC mobilization.
authors.forename: Lizhen
authors.initials: L
authors.lastname: Liu
authors.forename: Qin
authors.initials: Q
authors.lastname: Yu
authors.forename: Kaimin
authors.initials: K
authors.lastname: Hu
authors.forename: Binsheng
authors.initials: B
authors.lastname: Wang
authors.forename: Yiran
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yulin
authors.initials: Y
authors.lastname: Xu
authors.forename: Shan
authors.initials: S
authors.lastname: Fu
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Yu
authors.forename: He
authors.initials: H
authors.lastname: Huang
mesh_terms: D026881:Acupuncture; D050156:Adipogenesis; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D019070:Cell Lineage; D002465:Cell Movement; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000032:analysis; D020219:Chondrogenesis; D004558:Electric Stimulation; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D016130:Immunophenotyping; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010012:Osteogenesis; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D054377:Chemokine CXCL12