title: Development of a novel noncapillary plasma microsampling device for ultra-low volume of blood collection.
abstract: The desire for serial microsampling in mice has led to extensive research in this field within the pharmaceutical industry. The ability to profile a compound's in vivo properties with less material and fewer mice has obvious advantages. A new device and workflow was developed at the Takeda Oncology site to allow scientists to isolate plasma from very low volumes of mouse blood (as low as 20 μl) collected using standard microsampling techniques. A side-by-side in vitro comparison of plasma concentrations was performed using this new device and conventional sampling methods with commercial and in-house molecules. The plasma concentrations of the molecules tested were very consistent between the conventional sampling techniques and this new device/workflow. In addition, several in-life studies have also been conducted to validate this new technique as a primary PK screening tool at the Takeda Boston. The new device is simple to use and very cost effective with the added benefit that no additional training is needed for the animal technicians and the same centrifuge equipment can be employed. This device can be used for blood volumes ranging from 20 to 100 μl enabling studies not just in rat and dog but more importantly in mice.
authors.affiliation: Discovery Analytical Research Group, Millennium Pharmaceuticals Inc., a wholly owned subsidiary of Takeda Pharmaceuticals Company Limited, 40 Landsdowne St., Cambridge, MA 02139, USA.
authors.forename: Ji
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Discovery Analytical Research Group, Millennium Pharmaceuticals Inc., a wholly owned subsidiary of Takeda Pharmaceuticals Company Limited, 40 Landsdowne St., Cambridge, MA 02139, USA.
authors.forename: David
authors.initials: D
authors.lastname: Lok
authors.affiliation: Discovery Analytical Research Group, Millennium Pharmaceuticals Inc., a wholly owned subsidiary of Takeda Pharmaceuticals Company Limited, 40 Landsdowne St., Cambridge, MA 02139, USA.
authors.forename: Jesse
authors.initials: J
authors.lastname: Gray
authors.affiliation: Discovery Analytical Research Group, Millennium Pharmaceuticals Inc., a wholly owned subsidiary of Takeda Pharmaceuticals Company Limited, 40 Landsdowne St., Cambridge, MA 02139, USA.
authors.forename: Steve
authors.initials: S
authors.lastname: Grossman
authors.affiliation: Discovery Analytical Research Group, Millennium Pharmaceuticals Inc., a wholly owned subsidiary of Takeda Pharmaceuticals Company Limited, 40 Landsdowne St., Cambridge, MA 02139, USA.
authors.forename: Matt
authors.initials: M
authors.lastname: Jones
mesh_terms: D000077716:Afatinib; D000818:Animals; D001800:Blood Specimen Collection / Q000295:instrumentation*; D004285:Dogs; D004867:Equipment Design; D051379:Mice; D008904:Miniaturization / Q000295:instrumentation; D011799:Quinazolines / Q000097:blood; D011838:Radiation-Sensitizing Agents / Q000032:analysis; D051381:Rats; D018401:Sample Size
chemical_list: D011799:Quinazolines; D011838:Radiation-Sensitizing Agents; D000077716:Afatinib
keywords: capillary microsampling (CMS); dried matrix spot (DMS); hematocrit; mean percentage difference (MPD); serial tail vein bleed