title: The fabrication of a label-free electrochemical immunosensor using Nafion/carbon nanotubes/charged pyridinecarboxaldehyde composite film.
abstract: A label-free electrochemical immunosensor based on Nafion/carbon nanotubes (CNTs)/charged pyridinecarboxaldehyde composite film was developed for the detection of hepatitis B surface antigen. Nafion/CNTs/charged pyridinecarboxaldehyde nanocomposites were prepared by dispersing charged pyridinecarboxaldehyde and CNTs in Nafion solution. The nanocomposites were cast on the electrode surface to form aldehyde-terminated composite film that can covalently bind antibody on the film without using other reagent. The immunosensor response was linearly changed with hepatitis B surface antigen concentration in the range from 0.1 to 25 ng ml(-1) with a detection limit (signal/noise ratio = 3) of 0.04 ng ml(-1). Some important advantages such as simple preparation, good stability, reproducibility, and selectivity of the immunosensor were achieved.
authors.affiliation: Department of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, PR China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, PR China.
authors.forename: Youming
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, PR China. Electronic address: sgyrab@163.com.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Shen
authors.affiliation: Department of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, PR China.
authors.forename: Sha
authors.initials: S
authors.lastname: Wang
mesh_terms: D055664:Electrochemical Techniques; D005465:Fluorocarbon Polymers / Q000737:chemistry*; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D007118:Immunoassay; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010316:Particle Size; D011725:Pyridines / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D005465:Fluorocarbon Polymers; D006514:Hepatitis B Surface Antigens; D037742:Nanotubes, Carbon; D011725:Pyridines; C051439:pyridinecarboxaldehyde; C040402:perfluorosulfonic acid
keywords: Antibody immobilization; Carbon nanotube; Charged pyridinecarboxaldehyde; Hepatitis B surface antigen; Nafion