title: Contrasting increased levels of serum amyloid A in patients with three different bone sarcomas: An indicator of tumor malignancy?
abstract: Sarcoma is a malignant tumor that originates from the bone or soft tissue. In this study, abundances of serum amyloid A (SAA) in patients with pleomorphic sarcoma (PS), chondrosarcoma (CS), and osteosarcoma (OS) were analyzed and compared with those from their respective age-matched healthy control subjects. Results obtained from our analysis by 2DE showed that the levels of SAA were markedly elevated in patients with PS and OS, which are highly metastatic, while in patients with CS, which is a less aggressive sarcoma, the increase appeared less pronounced. A similar trend of altered abundances was also observed when the levels of SAA in the subjects were estimated using Western blot, ELISA, and multiple-reaction monitoring analyses. Absolute quantification using multiple-reaction monitoring further demonstrated that the increased abundance of SAA in patients with PS, OS, and CS was mainly attributed to isoform SAA1. In view of the different degrees of tumor malignancy in PS, OS, and CS, our data suggest their apparent correlation with the levels of SAA in the patients.
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Wan Izlina
authors.initials: WI
authors.lastname: Wan-Ibrahim
authors.affiliation: Medical Biotechnology Laboratory, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Ali
authors.initials: A
authors.lastname: Ashrafzadeh
authors.affiliation: Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Vivek Ajit
authors.initials: VA
authors.lastname: Singh
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.|University of Malaya Centre of Proteomics Research (UMCPR), University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Onn Haji
authors.initials: OH
authors.lastname: Hashim
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. terisar@um.edu.my, puteri@ummc.edu.my.|University of Malaya Centre of Proteomics Research (UMCPR), University of Malaya, Kuala Lumpur, Malaysia. terisar@um.edu.my, puteri@ummc.edu.my.
authors.forename: Puteri Shafinaz
authors.initials: PS
authors.lastname: Abdul-Rahman
mesh_terms: D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D015153:Blotting, Western; D001859:Bone Neoplasms / Q000097:blood / Q000473:pathology*; D016022:Case-Control Studies; D002813:Chondrosarcoma / Q000097:blood / Q000473:pathology*; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D012516:Osteosarcoma / Q000097:blood / Q000473:pathology*; D000685:Serum Amyloid A Protein / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000685:Serum Amyloid A Protein
keywords: Chondrosarcoma; Osteosarcoma; Pleomorphic sarcoma; Proteomics; Serum amyloid A