title: Investigating fibroblast cells under "safe" and "injurious" blue-light exposure by holographic microscopy.
abstract: The exposure to visible light has been shown to exert various biological effects, such as erythema and retinal degeneration. However, the phototoxicity mechanisms in living cells are still not well understood. Here we report a study on the temporal evolution of cell morphology and volume during blue light exposure. Blue laser irradiation is switched during the operation of a digital holography (DH) microscope between what we call here "safe" and "injurious" exposure (SE & IE). The results reveal a behaviour that is typical of necrotic cells, with early swelling and successive leakage of the intracellular liquids when the laser is set in the "injurious" operation. In the phototoxicity investigation reported here the light dose modulation is performed through the very same laser light source adopted for monitoring the cell's behaviour by digital holographic microscope. We believe the approach may open the route to a deep investigation of light-cell interactions, with information about death pathways and threshold conditions between healthy and damaged cells when subjected to light-exposure. 3D Morphology and quantitative phase information from late stage of necrosis cell death.
authors.affiliation: National Council of Research, Institute of Applied Science & Intelligent Systems (ISASI) 'E. Caianiello', Via Campi Flegrei 34, 80078, Pozzuoli (NA), Italy.|Department of Chemical, Materials and Industrial Production Engineering, University of Naples Federico II, P. le Tecchio 80, 80125, Napoli, Italy.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Calabuig
authors.affiliation: National Council of Research, Institute of Applied Science & Intelligent Systems (ISASI) 'E. Caianiello', Via Campi Flegrei 34, 80078, Pozzuoli (NA), Italy.|Department of Chemical, Materials and Industrial Production Engineering, University of Naples Federico II, P. le Tecchio 80, 80125, Napoli, Italy.
authors.forename: Martina
authors.initials: M
authors.lastname: Mugnano
authors.affiliation: National Council of Research, Institute of Applied Science & Intelligent Systems (ISASI) 'E. Caianiello', Via Campi Flegrei 34, 80078, Pozzuoli (NA), Italy.
authors.forename: Lisa
authors.initials: L
authors.lastname: Miccio
authors.affiliation: National Council of Research, Institute of Applied Science & Intelligent Systems (ISASI) 'E. Caianiello', Via Campi Flegrei 34, 80078, Pozzuoli (NA), Italy.
authors.forename: Simonetta
authors.initials: S
authors.lastname: Grilli
authors.affiliation: National Council of Research, Institute of Applied Science & Intelligent Systems (ISASI) 'E. Caianiello', Via Campi Flegrei 34, 80078, Pozzuoli (NA), Italy.
authors.forename: Pietro
authors.initials: P
authors.lastname: Ferraro
mesh_terms: D048429:Cell Size; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology*; D006696:Holography; D006801:Humans; D007834:Lasers; D008027:Light / Q000009:adverse effects*; D008853:Microscopy
keywords: cell morphology; cell photo-toxicity; cell volume changes; holographic microscopy