title: Effects of dietary supplementations with the fibrous root of Rhizoma Coptidis and its main alkaloids on non-specific immunity and disease resistance of common carp.
abstract: The effects of fibrous root of Rhizoma Coptidis (FRC) and its main alkaloids on non-specific immunity and disease resistance of common carp were investigated. The fish were randomly assigned to seven groups: normal control group (NC), groups treated with 12.5 g/kg FRC (FRC-L), 25 g/kg FRC (FRC-M), 50 g/kg FRC (FRC-H), 0.78 g/kg total alkaloids (TA), 0.78 g/kg berberine (BBR), and 0.78 g/kg coptisine (Cop), respectively. Results showed that the activities of myeloperoxidase, lysozyme and respiratory burst were significantly elevated after treated with FRC-M, FRC-H, TA, BBR and Cop, and the complement C3 level and phagocytic activity were significantly increased in FRC-M, TA and BBR treated groups compared with NC group. The real-time PCR analysis indicated that FRC, TA, BBR and Cop could up-regulate the mRNA expression of IL-1β, TNF-α, lysozyme-c and C3, but down-regulate that of IL-10 in the head kidney of common carp. Besides, FRC-M, FRC-H, TA, BBR and Cop significantly enhanced the survival rate of common carp infected with Aeromonas hydrophila, when compared to NC group. It was concluded that the FRC could enhance the non-specific immunity and disease resistance of common carp and the main alkaloids might contribute to these effects.
authors.affiliation: School of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhou
authors.affiliation: School of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
authors.forename: Yaozong
authors.initials: Y
authors.lastname: Peng
authors.affiliation: School of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
authors.forename: Kai
authors.initials: K
authors.lastname: He
authors.affiliation: School of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Huang
authors.affiliation: School of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
authors.forename: Shaoxia
authors.initials: S
authors.lastname: Mou
authors.affiliation: School of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
authors.forename: Min
authors.initials: M
authors.lastname: Feng
authors.affiliation: School of Life Sciences, Southwest University, Chongqing 400715, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Han
authors.affiliation: School of Life Sciences, Southwest University, Chongqing 400715, China; Chongqing Productivity Promotion Center for Modern Chinese Medicine, Chongqing 400715, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Ye
authors.affiliation: School of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China; Chongqing Productivity Promotion Center for Modern Chinese Medicine, Chongqing 400715, China. Electronic address: xuegangli@swu.edu.cn.
authors.forename: Xuegang
authors.initials: X
authors.lastname: Li
mesh_terms: D016980:Aeromonas hydrophila; D000470:Alkaloids / Q000008:administration & dosage; D000821:Animal Feed; D000818:Animals; D002347:Carps; D039323:Coptis; D019587:Dietary Supplements; D060467:Disease Resistance; D005393:Fish Diseases / Q000276:immunology* / Q000517:prevention & control; D016905:Gram-Negative Bacterial Infections / Q000517:prevention & control / Q000662:veterinary; D010946:Plants, Medicinal
chemical_list: D000470:Alkaloids
keywords: Aeromonas hydrophila; Disease resistance; Fibrous root of Rhizoma Coptidis; Non-specific immunity