title: The Use of Thiol/Disulfide as a Novel Marker in Premature Ovarian Failure.
abstract: The study aimed to compare the dynamic thiol/disulfide homeostasis between patients with premature ovarian failure (POF) and healthy women. A total of 77 women, 40 POF and 37 healthy controls, were recruited from a university hospital between December 2013 and June 2015. Blood samples were taken from patients to evaluate follicle stimulating hormone (FSH), luteinizing hormone, estradiol and thiol/disulfide levels. A new, fully automated method was used to measure plasma thiol, total thiol and disulfide levels. Disulfide levels, disulfide/native thiol and disulfide/total thiol ratios were significantly increased, native thiol/total thiol levels were significantly decreased in POF patients compared to controls (p < 0.001). FSH was negatively correlated with native thiol and native thiol/total thiol levels and positively with disulfide, disulfide/native thiol, and disulfide/total thiol levels. This is the first study demonstrating the thiol/disulfide homeostasis in women with POF and may help us understanding the pathophysiology.
authors.affiliation: Department of Obstetrics and Gynecology, Bulent Ecevıt Unıversıty School of Medicine, Zonguldak, Turkey.
authors.forename: Hatice
authors.initials: H
authors.lastname: Isik
authors.forename: Ahmet
authors.initials: A
authors.lastname: Sahbaz
authors.forename: Hakan
authors.initials: H
authors.lastname: Timur
authors.forename: Oner
authors.initials: O
authors.lastname: Aynioglu
authors.forename: Sule
authors.initials: S
authors.lastname: Atalay Mert
authors.forename: Ahmet Rıfat
authors.initials: AR
authors.lastname: Balık
authors.forename: Ozcan
authors.initials: O
authors.lastname: Erel
authors.forename: Muge
authors.initials: M
authors.lastname: Harma
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D004220:Disulfides / Q000097:blood*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood*; D006801:Humans; D016649:Primary Ovarian Insufficiency / Q000097:blood*; D013438:Sulfhydryl Compounds / Q000097:blood*
chemical_list: D004220:Disulfides; D013438:Sulfhydryl Compounds; D005640:Follicle Stimulating Hormone