title: Systematic review of next-generation sequencing simulators: computational tools, features and perspectives.
abstract: High-throughput next-generation sequencing (NGS) technologies have rapidly generated a large volume of genomic data. To aid the development and evaluation of new statistical models and computational methods, NGS-based simulators have been proposed to construct better experimental workflows. However, the comparative performance of these NGS simulators remains unclear. In this review, we conducted a comprehensive investigation of NGS simulators for various sequencing techniques, including DNA sequencing, metagenomic sequencing, RNA-seq, ChIP-seq and bisulfite sequencing for methylation.
authors.forename: Min
authors.initials: M
authors.lastname: Zhao
authors.forename: Di
authors.initials: D
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Qu
mesh_terms: D023281:Genomics / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D017423:Sequence Analysis, RNA / Q000379:methods
keywords: high-throughput next-generation sequencing; simulator