title: Repulsive cues combined with physical barriers and cell-cell adhesion determine progenitor cell positioning during organogenesis.
abstract: The precise positioning of organ progenitor cells constitutes an essential, yet poorly understood step during organogenesis. Using primordial germ cells that participate in gonad formation, we present the developmental mechanisms maintaining a motile progenitor cell population at the site where the organ develops. Employing high-resolution live-cell microscopy, we find that repulsive cues coupled with physical barriers confine the cells to the correct bilateral positions. This analysis revealed that cell polarity changes on interaction with the physical barrier and that the establishment of compact clusters involves increased cell-cell interaction time. Using particle-based simulations, we demonstrate the role of reflecting barriers, from which cells turn away on contact, and the importance of proper cell-cell adhesion level for maintaining the tight cell clusters and their correct positioning at the target region. The combination of these developmental and cellular mechanisms prevents organ fusion, controls organ positioning and is thus critical for its proper function.
authors.affiliation: Institute for Cell Biology, ZMBE, Von-Esmarch-Street 56, 48149 Muenster, Germany.
authors.forename: Azadeh
authors.initials: A
authors.lastname: Paksa
authors.affiliation: Institute for Cell Biology, ZMBE, Von-Esmarch-Street 56, 48149 Muenster, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Bandemer
authors.forename: Burkhard
authors.initials: B
authors.lastname: Hoeckendorf
authors.affiliation: Department of Chemical Physics, Weizmann Institute of Science, Rehovot 76100, Israel.
authors.forename: Nitzan
authors.initials: N
authors.lastname: Razin
authors.affiliation: Institute for Cell Biology, ZMBE, Von-Esmarch-Street 56, 48149 Muenster, Germany.
authors.forename: Katsiaryna
authors.initials: K
authors.lastname: Tarbashevich
authors.affiliation: Germ Cell Development, Max-Planck Institute of Biophysical Chemistry, Am Fassberg 11, 37070 Göttingen, Germany.
authors.forename: Sofia
authors.initials: S
authors.lastname: Minina
authors.affiliation: Institute for Cell Biology, ZMBE, Von-Esmarch-Street 56, 48149 Muenster, Germany.
authors.forename: Dana
authors.initials: D
authors.lastname: Meyen
authors.affiliation: Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, Von-Esmarch-Strasse 54, 48149 Muenster, Germany.
authors.forename: Antonio
authors.initials: A
authors.lastname: Biundo
authors.affiliation: Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, Von-Esmarch-Strasse 54, 48149 Muenster, Germany.
authors.forename: Sebastian A
authors.initials: SA
authors.lastname: Leidel
authors.affiliation: USR3695 BioEmergences, CNRS, Université Paris-Saclay, Avenue de la Terrasse, 91190 Gif-sur-Yvette, France.
authors.forename: Nadine
authors.initials: N
authors.lastname: Peyrieras
authors.affiliation: Department of Chemical Physics, Weizmann Institute of Science, Rehovot 76100, Israel.
authors.forename: Nir S
authors.initials: NS
authors.lastname: Gov
authors.forename: Philipp J
authors.initials: PJ
authors.lastname: Keller
authors.affiliation: Institute for Cell Biology, ZMBE, Von-Esmarch-Street 56, 48149 Muenster, Germany.
authors.forename: Erez
authors.initials: E
authors.lastname: Raz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D064113:CRISPR-Cas Systems / Q000235:genetics; D002448:Cell Adhesion / Q000235:genetics; D002465:Cell Movement / Q000235:genetics; D004064:Digestive System / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D053595:Embryonic Stem Cells / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods; D018507:Gene Expression Regulation, Developmental; D006066:Gonads / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D008667:Metalloproteins / Q000145:classification / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D038081:Organogenesis / Q000235:genetics*; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D059008:Time-Lapse Imaging; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*; D029961:Zebrafish Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism
chemical_list: C530880:Lpp protein, zebrafish; D008667:Metalloproteins; D029961:Zebrafish Proteins