title: Increased Granulocyte Heparanase Activity in Neutrophils from Patients with Lupus Nephritis and Idiopathic Membranous Nephropathy.
abstract: Heparanase is a β-glucuronidase that cleaves sugar chains of heparan sulfate proteoglycans. It is believed that heparanase may be involved in the pathogenesis of proteinuria. The aim of this study was to assess the significance of heparanase in the pathogenesis of particular glomerulonephritis types. The evaluation of heparanase activity in serum, urine, and granulocytes and superoxide dismutase (SOD) activity in granulocytes of patients with lupus nephritis (n = 17), membranous nephropathy (n = 11), IgA nephropathy (n = 12), focal and segmental glomerulosclerosis (n = 18), mesangiocapillary glomerulonephritis (n = 12) and in 19 healthy volunteers were performed. The heparanase activity in granulocytes of patients with lupus nephritis and membranous nephropathy was higher than heparanase activity in granulocytes in the control group (p = 0.02 in both cases). This is the first observation of this phenomenon. There was no difference between SOD activity in granulocytes of patients with all assessed types of glomerulonephritis and the control group. A positive correlation between heparanase activity in urine and double-strain DNA antibodies (r = 0.51; p = 0.04), and reverse correlations between heparanase in urine and hemolytic activity of the complement (r = -0.57; p = 0.03) in the lupus nephritis group, and between heparanase activity in granulocytes and serum total protein level (r = -0.69; p = 0.02) in membranous nephropathy were observed. Increase in heparanase activity without changes in superoxide dismutase activity in the granulocytes from patients with lupus nephritis and membranous nephropathy was observed. It may be used as one of the markers of these disease activities.
authors.affiliation: Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, Wroclaw, Poland. mszymc@wp.pl.
authors.forename: Maciej
authors.initials: M
authors.lastname: Szymczak
authors.affiliation: Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, Wroclaw, Poland.
authors.forename: Jakub
authors.initials: J
authors.lastname: Kuźniar
authors.affiliation: Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, Wroclaw, Poland.
authors.forename: Wacław
authors.initials: W
authors.lastname: Kopeć
authors.affiliation: Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, Wroclaw, Poland.
authors.forename: Marcelina
authors.initials: M
authors.lastname: Żabińska
authors.affiliation: Department of Toxicology, Wroclaw Medical University, Wroclaw, Poland.
authors.forename: Zofia
authors.initials: Z
authors.lastname: Marchewka
authors.affiliation: Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, Wroclaw, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kościelska-Kasprzak
authors.affiliation: Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, Wroclaw, Poland.
authors.forename: Marian
authors.initials: M
authors.lastname: Klinger
mesh_terms: D000906:Antibodies / Q000097:blood; D005260:Female; D015433:Glomerulonephritis, Membranous / Q000097:blood* / Q000276:immunology*; D005966:Glucuronidase / Q000097:blood / Q000378:metabolism* / Q000652:urine; D006098:Granulocytes / Q000201:enzymology*; D006801:Humans; D008181:Lupus Nephritis / Q000097:blood* / Q000276:immunology*; D008297:Male; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism; D000099175:Heparanase
chemical_list: D000906:Antibodies; D005966:Glucuronidase; D013482:Superoxide Dismutase; D000099175:Heparanase
keywords: Glomerular diseases; Granulocytes; Heparanase