title: Inhibition of miR-21 in glioma cells using catalytic nucleic acids.
abstract: Despite tremendous efforts worldwide, glioblastoma multiforme (GBM) remains a deadly disease for which no cure is available and prognosis is very bad. Recently, miR-21 has emerged as a key omnipotent player in carcinogenesis, including brain tumors. It is recognized as an indicator of glioma prognosis and a prosperous target for anti-tumor therapy. Here we show that rationally designed hammerhead ribozymes and DNAzymes can target miR-21 and/or its precursors. They decrease miR-21 level, and thus silence this oncomiR functions. We demonstrated that anti-miRNA catalytic nucleic acids show a novel terrific arsenal for specific and effective combat against diseases with elevated cellular miR-21 content, such as brain tumors.
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Belter
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Rolle
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Piwecka
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Fedoruk-Wyszomirska
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.
authors.forename: Mirosława Z
authors.initials: MZ
authors.lastname: Naskręt-Barciszewska
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Barciszewski
mesh_terms: D001932:Brain Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D021881:DNA, Catalytic / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics*; D006801:Humans; D006868:Hydrolysis; D035683:MicroRNAs / Q000235:genetics*; D016337:RNA, Catalytic / Q000235:genetics*
chemical_list: D021881:DNA, Catalytic; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D016337:RNA, Catalytic