title: [Role of miR-663 in acute renal graft rejection: an in vitro study].
abstract: OBJECTIVE
  To compare the serum miR-663 levels in renal transplant patients with and without acute rejection (AR) and explore the role of miR-663 acute renal graft rejection.
  
  
  METHODS
  Real time-PCR was used to determine serum miR-663 levels in renal transplant recipients with and without AR. MTT assay and Annexin V-FITC assay were employed to examine the viability and apoptosis of human renal glomerular endothelial cells (HRGEC) treated with a miR-663 mimic or a miR-663 inhibitor, and ELISA was performed to detect the expression of inflammation-related cytokines including IL-6, IFN-γ, CCL-2 and TNF-α in the cells. Transwell assay was used to examine the effect of miR-663 mimic and miR-663 inhibitor on the chemotactic capability of macrophages.
  
  
  RESULTS
  Serum miR-663 level was significantly higher in renal transplant recipients with AR than in those without AR. The miR-663 mimic significantly inhibited the viability of HRGECs and increase the cell apoptosis rate, while miR-663 inhibitor suppressed the cell apoptosis. The miR-663 mimic increased the expression levels of inflammation-related cytokines and enhanced the chemotactic capability of macrophages.
  
  
  CONCLUSIONS
  miR-663 might play important roles in acute renal graft rejection and may become a therapeutic target for treating AR.
authors.affiliation: Department of OrganTransplantation, ZhujiangHospital, Southern Medical University Guangzhou 510282, China.E-mail: liuxy@fimmu.com.
authors.forename: Xiao-You
authors.initials: XY
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Liang
authors.forename: Yong-Guang
authors.initials: YG
authors.lastname: Liu
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Hu
authors.forename: Zheng-Yao
authors.initials: ZY
authors.lastname: Jiang
authors.forename: Ze-Feng
authors.initials: ZF
authors.lastname: Guo
mesh_terms: D017209:Apoptosis; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology; D006084:Graft Rejection / Q000097:blood*; D006801:Humans; D007678:Kidney Glomerulus / Q000166:cytology; D016030:Kidney Transplantation; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D035683:MicroRNAs / Q000097:blood*
chemical_list: D016207:Cytokines; C552243:MIRN663 microRNA, human; D035683:MicroRNAs