title: Immunogenicity Analysis of a Novel Subunit Vaccine Candidate Molecule-Recombinant L7/L12 Ribosomal Protein of Brucella suis.
abstract: Brucella was an intracellular parasite, which could infect special livestock and humans. After infected by Brucella, livestock's reproductive system could be affected and destroyed resulting in huge economic losses. More seriously, it could be contagious from livestock to humans. So far, there is no available vaccine which is safe enough for humans. On this point, subunit vaccine has become the new breakthrough of conquering brucellosis. In this study, Brucella rL7/L12-BLS fusion protein was used as an antigen to immunize rabbits to detect the immunogenicity. The results of antibody level testing assay of rabbit antiserum indicated rL7/L12-BLS fusion protein could elicit rabbits to produce high-level IgG. And gamma interferon (IFN-γ) concentrations in rabbit antiserum were obviously up-regulated in both the rL7/L12 group and rL7/L12-BLS group. Besides, the results of quantitative real-time PCR (qRT-PCR) showed the IFN-γ gene's expression levels of both the rL7/L12 group and rL7/L12-BLS group were obviously up-regulated. All these results suggested Brucella L7/L12 protein was an ideal subunit vaccine candidate and possessed good immunogenicity. And Brucella lumazine synthase (BLS) molecule was a favorable transport vector for antigenic protein.
authors.affiliation: School of Life Science and Technology, Inner Mongolia University of Science and Technology, Arding Street No.7, Kundulun District, Baotou, Inner Mongolia Autonomous Region, 014010, China. nmdzq1981@163.com.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Du
authors.affiliation: Baotou Tumour Hospital, Baotou, Inner Mongolia Autonomous Region, 014010, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Life Science and Technology, Inner Mongolia University of Science and Technology, Arding Street No.7, Kundulun District, Baotou, Inner Mongolia Autonomous Region, 014010, China.
authors.forename: Jian-Ying
authors.initials: JY
authors.lastname: Wang
mesh_terms: D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D041021:Brucella suis / Q000235:genetics / Q000276:immunology*; D018076:DNA, Complementary / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005798:Genes, Bacterial; D007114:Immunization; D007074:Immunoglobulin G / Q000378:metabolism; D007371:Interferon-gamma / Q000096:biosynthesis / Q000097:blood / Q000235:genetics; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology* / Q000302:isolation & purification; D012269:Ribosomal Proteins / Q000235:genetics / Q000276:immunology*; D022223:Vaccines, Subunit / Q000276:immunology*
chemical_list: D018076:DNA, Complementary; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; D022223:Vaccines, Subunit; C016700:ribosomal protein L7-L12; D007371:Interferon-gamma
keywords: Brucella; Immunogenicity; L7/L12 ribosomal protein; Subunit vaccine candidate