title: Update of OECD DART guidelines with endocrine disruptor relevant endpoints: Practical considerations.
abstract: In 1998, the OECD initiated a high-priority project aimed at revising existing test guidelines and developing new test guidelines for screening of potential endocrine disruptors. In 2011, OECD 443 was adopted, and in 2015 OECD 421 and OECD 422 were updated with endocrine disruptor relevant endpoints. A feasibility study for the enhancement of OECD 414 with endocrine disruptor relevant endpoints is currently ongoing. The addition of these endpoints is considered crucial for gaining more information on endocrine disruptor potency of tested chemicals, however it should be noted that these additions have a major impact on the study designs and give rise to several practical challenges. The aim of this review is to discuss important aspects of these challenging study designs and to share our knowledge on their implementation in our laboratory. Together, this review can be used as guidance for other laboratories, study monitors and registration officers.
authors.affiliation: WIL Research Europe B.V., 's-Hertogenbosch, The Netherlands. Electronic address: manon.beekhuijzen@wilresearch.com.
authors.forename: Manon
authors.initials: M
authors.lastname: Beekhuijzen
authors.affiliation: WIL Research Europe B.V., 's-Hertogenbosch, The Netherlands.
authors.forename: Francois
authors.initials: F
authors.lastname: van Otterdijk
authors.affiliation: WIL Research Europe B.V., 's-Hertogenbosch, The Netherlands.
authors.forename: Willemien
authors.initials: W
authors.lastname: Wieland
authors.affiliation: WIL Research Europe B.V., 's-Hertogenbosch, The Netherlands.
authors.forename: Miranda
authors.initials: M
authors.lastname: van Tuyl
authors.affiliation: WIL Research Europe B.V., 's-Hertogenbosch, The Netherlands.
authors.forename: Robert Pels
authors.initials: RP
authors.lastname: Rijcken
authors.affiliation: WIL Research Europe B.V., 's-Hertogenbosch, The Netherlands.
authors.forename: Birgit
authors.initials: B
authors.lastname: Peter
authors.affiliation: WIL Research Europe B.V., 's-Hertogenbosch, The Netherlands.
authors.forename: Harry
authors.initials: H
authors.lastname: Emmen
mesh_terms: D000818:Animals; D052244:Endocrine Disruptors / Q000633:toxicity*; D023381:Endpoint Determination / Q000639:trends*; D017408:Guidelines as Topic / Q000592:standards*; D012098:Reproduction / Q000187:drug effects*; D012107:Research Design; D018675:Toxicity Tests / Q000379:methods* / Q000639:trends
chemical_list: D052244:Endocrine Disruptors
keywords: EOGRTS; Endocrine disruption; Extended one-generation reproductive toxicity study; OECD 421; OECD 422; OECD 443; Regulatory; Repro screening