title: Characterization and viral susceptibility of a brain cell line from brown-marbled grouper Epinephelus fuscoguttatus (Forsskål) with persistent betanodavirus infection.
abstract: A continuous cell line designated BMGB (brown-marbled grouper brain) was established from the brain tissues of the brown-marbled grouper Epinephelus fuscoguttatus and characterized. BMGB cells were identified as astroglial progenitor cells because they expressed glial fibrillary acidic protein and keratin and were persistently infected by betanodavirus, as confirmed through immunocytochemistry, polymerase chain reaction and immunoblot analyses. Because few intact virions were present in the BMGB cell culture fluid, the cytopathic effect (CPE) was not observed when the culture fluid was inoculated with GBC1 cells. However, BMGB cells displayed typical CPE after infection with additional betanodavirus, megalocytivirus and chum salmon reovirus. BMGB cells showed low myxovirus resistance (Mx) protein expression, which increased following betanodavirus and reovirus infection. Because the cells contained several unusual or degraded viral proteins, the persistent infection of betanodavirus in the BMGB cells may have resulted from a mechanism that destroys the viral proteins rather than the result of Mx protein expression. Despite the persistent betanodavirus infection, BMGB cells proliferated in a manner similar to other normal tropic fish cells and supported the propagation of several piscine viruses; however, the yield was lower than that of normal cells. The BMGB cells will be useful for investigating virus and host cell interaction.
authors.affiliation: Department of Life Sciences, National University of Kaohsiung, Kaohsiung, Taiwan. wenchiumin@nuk.edu.tw.
authors.forename: C M
authors.initials: CM
authors.lastname: Wen
mesh_terms: D000818:Animals; D001492:Bass; D001921:Brain / Q000821:virology; D002460:Cell Line; D004198:Disease Susceptibility / Q000662:veterinary / Q000821:virology; D005393:Fish Diseases / Q000235:genetics / Q000821:virology*; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D064547:Myxovirus Resistance Proteins / Q000235:genetics / Q000378:metabolism; D030381:Nodaviridae / Q000502:physiology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012327:RNA Virus Infections / Q000235:genetics / Q000662:veterinary* / Q000821:virology; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D029941:Fish Proteins; D064547:Myxovirus Resistance Proteins
keywords: betanodavirus; cell line; grouper; megalocytivirus; myxovirus resistance protein