title: [The impact of S.gordonii on P. gingivalis on the form of biofilm].
abstract: OBJECTIVE
  To investigate the impact of S.gordonii on the ultrastructure of P. gingivalis biofilm and on the amount of P. gingivalis in biofilm.
  
  
  METHODS
  P. gingivalis and/or S.gordonii grew on the culture slides to form single P. gingivalis biofilm and heterotypic biofilm of P.gingivalis-S.gordonii. Then the ultrastructure of the 2 kinds of film were examined by scanning electron microscope, and the amount of P. gingivalis in the biofilm was detected by qPCR. Statistical analysis was performed using pair t test with SPSS 13.0 statistical package.
  
  
  RESULTS
  At 72 h, the amount of heterotypic biofilm was much more than that of the single P. gingivalis biofilm. Moreover, the structure of the heterotypic biofilm was more regular and with more pore space compared to the single P. gingivalis biofilm. Compared to single P. gingivalis biofilm, the amount of P. gingivalis in heterotypic biofilm was 5.4, 3.8 and 4.4 fold at 24, 48 and 72 h, respectively.
  
  
  CONCLUSIONS
  The growth of P. gingivalis was promoted by S. gordonii in the form of heterotypic biofilm compared to single P. gingivalis biofilm.
authors.affiliation: Department of Periodontics, School of Stomatology, China Medical University, Liaoning Institute of Dental Research. Shenyang 110002, Liaoning Province, China. E-mail:juliyia@163.com.
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: Zhang
authors.affiliation: Department of Periodontics, School of Stomatology, China Medical University, Liaoning Institute of Dental Research. Shenyang 110002, Liaoning Province, China.
authors.forename: Jing-bo
authors.initials: JB
authors.lastname: Liu
authors.affiliation: Department of Periodontics, School of Stomatology, China Medical University, Liaoning Institute of Dental Research. Shenyang 110002, Liaoning Province, China.
authors.forename: Ya-ping
authors.initials: YP
authors.lastname: Pan
authors.affiliation: Department of Periodontics, School of Stomatology, China Medical University, Liaoning Institute of Dental Research. Shenyang 110002, Liaoning Province, China.
authors.forename: Jia-yu
authors.initials: JY
authors.lastname: Pan
authors.affiliation: Department of Periodontics, School of Stomatology, China Medical University, Liaoning Institute of Dental Research. Shenyang 110002, Liaoning Province, China.
authors.forename: Qiu-fang
authors.initials: QF
authors.lastname: Xu
mesh_terms: D018441:Biofilms; D016966:Porphyromonas gingivalis / Q000502:physiology*; D054773:Streptococcus gordonii / Q000502:physiology*