title: Use of Microfluidic Technology to Monitor the Differentiation and Migration of Human ESC-Derived Neural Cells.
abstract: Microfluidics forms the basis of unique experimental approaches that visualize the development of neural structure using micro-scale devices and aids the guidance of neurite growth in an axonal isolation compartment. We utilized microfluidics technology to monitor the differentiation and migration of neural cells derived from human embryonic stems cells (hESC). We cocultured hESC with PA6 stromal cells and isolated neural rosette-like structures, which subsequently formed neurospheres in a suspension culture. We found that Tuj1-positive neural cells but not nestin-positive neural precursor cells (NPC) were able to enter the microfluidics grooves (microchannels), suggesting a neural cell-migratory capacity that was dependent on neuronal differentiation. We also showed that bundles of axons formed and extended into the microchannels.Taken together, these results demonstrated that microfluidics technology can provide useful tools to study neurite outgrowth and axon guidance of neural cells, which are derived from human embryonic stem cells.
authors.affiliation: CHA Stem Cell Institute, Department of Biomedical Science, CHA University, Gyeonggi-do, Republic of Korea.
authors.forename: Jiwoo
authors.initials: J
authors.lastname: Bae
authors.affiliation: CHA Stem Cell Institute, Department of Biomedical Science, CHA University, Gyeonggi-do, Republic of Korea.
authors.forename: Nayeon
authors.initials: N
authors.lastname: Lee
authors.affiliation: CHA Stem Cell Institute, Department of Biomedical Science, CHA University, Gyeonggi-do, Republic of Korea.
authors.forename: Wankyu
authors.initials: W
authors.lastname: Choi
authors.affiliation: CHA Stem Cell Institute, Department of Biomedical Science, CHA University, Gyeonggi-do, Republic of Korea.
authors.forename: Suji
authors.initials: S
authors.lastname: Lee
authors.affiliation: CHA Stem Cell Institute, Department of Biomedical Science, CHA University, Gyeonggi-do, Republic of Korea.
authors.forename: Jung Jae
authors.initials: JJ
authors.lastname: Ko
authors.affiliation: Research Center for Integrated Cellulomics, KRIBB, Daejeon, 305-806, Republic of Korea.
authors.forename: Baek Soo
authors.initials: BS
authors.lastname: Han
authors.affiliation: Research Center for Integrated Cellulomics, KRIBB, Daejeon, 305-806, Republic of Korea.
authors.forename: Sang Chul
authors.initials: SC
authors.lastname: Lee
authors.affiliation: School of Mechanical and Aerospace Engineering, Seoul National University, Seoul, Republic of Korea.
authors.forename: Noo Li
authors.initials: NL
authors.lastname: Jeon
authors.affiliation: CHA Stem Cell Institute, Department of Biomedical Science, CHA University, Rm 604, CHA Bio Complex, 335 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-400, Republic of Korea. jsong@cha.ac.kr.
authors.forename: Jihwan
authors.initials: J
authors.lastname: Song
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002454:Cell Differentiation; D002460:Cell Line; D002465:Cell Movement; D018920:Coculture Techniques / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D000066449:Human Embryonic Stem Cells / Q000166:cytology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D056656:Lab-On-A-Chip Devices; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology*; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology*
keywords: Human embryonic stem cells (hESCs); Mature neurons; Microfluidic devices; Neural differentiation; Neural precursor cells (NPCs); Neurite outgrowth