title: Design of antioxidative biointerface for separation of hematopoietic stem cells with high maintenance of undifferentiated phenotype.
abstract: During cell cultivation, excessively generated reactive oxygen species (ROS) affect cellular properties and functions. Although cell cultivation media contain several types of low-molecular-weight antioxidants, these small antioxidants are internalized into the mitochondria and they disrupt regulated redox balance. Here, we developed a novel biointerface that effectively eliminates ROS on a cell culture surface. Poly(ethylene glycol)-b-poly[4-(2,2,6,6-tetramethylpiperidine-1-oxyl)aminomethylstyrene] (PEG-b-PMNT) was synthesized and covalently coated on a carboxyl group-activated culture dish using sec-amino groups on a PMNT segment followed by immobilization of anti-CD34 antibodies. CD34-positive hematopoietic stem progenitor cells (HSPCs) were separated from mice fetal liver cells using our polymer-coated cell culture dish. The separated HSPCs possessed intact mitochondrial membrane potential compared with those in the conventional cell cultivation system. In addition, the expression level of CD34 was maintained for an extended period on our culture dish with the antioxidative biointerface. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2080-2085, 2016.
authors.affiliation: Department of Materials Sciences, Graduate School of Pure and Applied Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8573, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Ikeda
authors.affiliation: Department of Materials Sciences, Graduate School of Pure and Applied Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8573, Japan.
authors.forename: Tomoki
authors.initials: T
authors.lastname: Yoshinari
authors.affiliation: Masters Program in Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8575, Japan.
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Miyoshi
authors.affiliation: Department of Materials Sciences, Graduate School of Pure and Applied Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8573, Japan.|Masters Program in Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8575, Japan.|Satellite Laboratory, International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute of Materials Science (NIMS), Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8573, Japan.
authors.forename: Yukio
authors.initials: Y
authors.lastname: Nagasaki
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry; D000975:Antioxidants / Q000494:pharmacology*; D001562:Benzimidazoles / Q000378:metabolism; D002232:Carbocyanines / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002469:Cell Separation / Q000379:methods*; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D010641:Phenotype; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism; D013343:Styrenes / Q000737:chemistry
chemical_list: D000906:Antibodies; D000975:Antioxidants; D001562:Benzimidazoles; D002232:Carbocyanines; D055767:Immobilized Proteins; D011108:Polymers; D017382:Reactive Oxygen Species; D013343:Styrenes; C068624:5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine; D011092:Polyethylene Glycols
keywords: PEG; hematopoietic stem cells; maintenance of undifferentiated phenotype; oxidative stress; scaffold