title: Effects of cultural medium on the formation and antitumor activity of polysaccharides by Cordyceps gunnii.
abstract: The effects of culture medium composition (i.e., carbon and nitrogen sources) on the growth of mycelia, molecular weight distribution and antitumor activity of intracellular polysaccharides (IPS) from Cordyceps gunnii were investigated. Sucrose and peptone were proved to be the best carbon and nitrogen sources for mycelia growth and remarkably improved IPS production. When the sucrose concentration was 2.0%, the mycelium yield reached up to 15.94±1.26 g/L, but with lower IPS yield; whereas the sucrose concentration was 4.5%, IPS yield reached to a maximum of 138.78±3.89 mg/100 mL. The effects of different carbon/nitrogen (C/N) ratios with equal amounts of carbon source matter on the mycelia and IPS formation were optimized. It found that the yield of mycelia and IPS were both reached to the highest at a C/N ratio of 10:3. In addition, the IPS had the highest macro molecular polysaccharide content and antitumor activity when sucrose concentration was 3.5% and the C/N ratio was 10:1.5. Thus, there was a positive correlation between molecular weight distribution and antitumor activity of IPS by C. gunnii.
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China; Tianjin Food Safety & Low Carbon Manufacturing Collaborative Innovation Center, Tianjin 300457, PR China. Electronic address: zhyuanzhu@tust.edu.cn.
authors.forename: Zhen-Yuan
authors.initials: ZY
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
authors.forename: Xiao-Cui
authors.initials: XC
authors.lastname: Liu
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Tang
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
authors.forename: Feng-Ying
authors.initials: FY
authors.lastname: Dong
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
authors.forename: Hui-Qing
authors.initials: HQ
authors.lastname: Sun
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, Insititut Parisien de Chimie Moléculaire, CNRS UMR 8232, 4 Place Jussieu, Paris 75005, France.
authors.forename: Yong-Min
authors.initials: YM
authors.lastname: Zhang
mesh_terms: D002244:Carbon / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology; D032661:Cordyceps / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D008970:Molecular Weight; D025282:Mycelium / Q000187:drug effects / Q000254:growth & development; D009584:Nitrogen / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology; D010461:Peptones / Q000378:metabolism / Q000494:pharmacology; D011134:Polysaccharides / Q000096:biosynthesis* / Q000494:pharmacology*; D013395:Sucrose / Q000378:metabolism / Q000494:pharmacology
chemical_list: D003470:Culture Media; D010461:Peptones; D011134:Polysaccharides; D013395:Sucrose; D002244:Carbon; D009584:Nitrogen
keywords: Antitumor activity; Cordyceps gunnii; Fermentation; Molecular weight distribution; Polysaccharide