title: Distance effects during polyprotein processing in the complementation between defective FMDV RNAs.
abstract: Passage of foot-and-mouth disease virus (FMDV) in BHK-21 cells resulted in the segmentation of the viral genome into two defective RNAs lacking part of either the L- or the capsid-coding region. The two RNAs are infectious by complementation. Electroporation of L-defective RNA in BHK-21 cells resulted in the accumulation of the precursor P3 located away from the deleted sequence. Expression of L in trans led to the processing of P3, indicating that there is a connection between L protease activity and the secondary cleavages carried out by 3C protease within P3. These results suggest that the complementation mechanism between defective RNAs is not restricted to supplying the L and capsid proteins but that distance effects on polyprotein processing events are also implicated.
authors.affiliation: Centro de Biología Molecular 'Severo Ochoa' (CSIC-UAM), Consejo Superior de Investigaciones Científicas (CSIC), Campus de Cantoblanco, Madrid, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Moreno
authors.affiliation: Liver Unit, Internal Medicine, Laboratory of Malalties Hepàtiques, Vall d'Hebron Institut de Recerca-Hospital Universitari Vall d´Hebron, (VHIR-HUVH), Universitat Autònoma de Barcelona, Barcelona, Spain.|Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Barcelona, Spain.|Centro de Biología Molecular 'Severo Ochoa' (CSIC-UAM), Consejo Superior de Investigaciones Científicas (CSIC), Campus de Cantoblanco, Madrid, Spain.
authors.forename: Celia
authors.initials: C
authors.lastname: Perales
mesh_terms: D000818:Animals; D002213:Capsid / Q000378:metabolism; D036022:Capsid Proteins / Q000235:genetics*; D002460:Cell Line; D006224:Cricetinae; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D003673:Defective Viruses / Q000235:genetics*; D018274:Electroporation; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics* / Q000378:metabolism; D016679:Genome, Viral / Q000235:genetics; D020815:Polyproteins / Q000235:genetics / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics*; D014779:Virus Replication / Q000235:genetics*
chemical_list: D036022:Capsid Proteins; D020815:Polyproteins; D012367:RNA, Viral; D012321:DNA-Directed RNA Polymerases