title: Hepatoprotective effect of resveratrol against ethanol-induced oxidative stress through induction of superoxide dismutasein vivoandin vitro.
abstract: The present study aimed to investigate the hepatoprotective effect of resveratrol (RSV) against ethanol-induced oxidative stressin vivo, and investigate the underlying mechanisms by which RSV exerts its anti-oxidative effects on hepatic cells. C57BL/6J mice were divided into four groups: Untreated control, ethanol-treated, RSV-treated, and ethanol + RSV-treated. The plasma lipid profile, hepatic lipid accumulation and antioxidative enzyme activities were analyzed. HepG2 cells were used as a cellular model to analyze the effects of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and peroxisome proliferator-activated receptors (PPARs) in the RSV-mediated protection of ethanol-induced oxidative stress. In C57BL/6J mice, ethanol caused a significant increase in plasma triglyceride levels and hepatic lipid accumulation (P<0.05), whereas RSV notably increased SOD activity. In HepG2 cells, SOD activity was enhanced in the RSV-treated HepG2 cells, whereas the activity of CAT and GPx was not affected. Western blot and quantitative polymerase chain reaction analyses demonstrated that RSV significantly increased SOD protein and mRNA expression levels (P<0.05). Using a transient transfection assay, PPARγ was observed to participate in the regulation of SOD gene expression in RSV-administered HepG2 cells. To conclude, the results from the present study suggest that RSV may contribute towards the protection of hepatic cells from ethanol-induced oxidative stress via the induction of SOD activity and gene expression.
authors.affiliation: Division of Gastroenterology and Hepatology, Department of Internal Medicine, Chang Gung Memorial Hospital, Puzi, Chiayi 61363, Taiwan, R.O.C.; Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan, R.O.C.; College of Medicine, Chang Gung University, Taoyuan 333, Taiwan, R.O.C.
authors.forename: Wei-Ming
authors.initials: WM
authors.lastname: Chen
authors.affiliation: Institute of Traditional Medicine, National Yang-Ming University, Taipei 11221, Taiwan, R.O.C.
authors.forename: Lee-Hsin
authors.initials: LH
authors.lastname: Shaw
authors.affiliation: Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan, R.O.C.
authors.forename: Pey-Jium
authors.initials: PJ
authors.lastname: Chang
authors.affiliation: Division of Gastroenterology and Hepatology, Department of Internal Medicine, Chang Gung Memorial Hospital, Puzi, Chiayi 61363, Taiwan, R.O.C.; College of Medicine, Chang Gung University, Taoyuan 333, Taiwan, R.O.C.
authors.forename: Shui-Yi
authors.initials: SY
authors.lastname: Tung
authors.affiliation: Division of Gastroenterology and Hepatology, Department of Internal Medicine, Chang Gung Memorial Hospital, Puzi, Chiayi 61363, Taiwan, R.O.C.; Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan, R.O.C.
authors.forename: Te-Sheng
authors.initials: TS
authors.lastname: Chang
authors.affiliation: Division of Gastroenterology and Hepatology, Department of Internal Medicine, Chang Gung Memorial Hospital, Puzi, Chiayi 61363, Taiwan, R.O.C.; Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan, R.O.C.
authors.forename: Chein-Heng
authors.initials: CH
authors.lastname: Shen
authors.affiliation: Division of Gastroenterology and Hepatology, Department of Internal Medicine, Chang Gung Memorial Hospital, Puzi, Chiayi 61363, Taiwan, R.O.C.; College of Medicine, Chang Gung University, Taoyuan 333, Taiwan, R.O.C.
authors.forename: Yung-Yu
authors.initials: YY
authors.lastname: Hsieh
authors.affiliation: Division of Gastroenterology and Hepatology, Department of Internal Medicine, Chang Gung Memorial Hospital, Puzi, Chiayi 61363, Taiwan, R.O.C.; College of Medicine, Chang Gung University, Taoyuan 333, Taiwan, R.O.C.
authors.forename: Kuo-Liang
authors.initials: KL
authors.lastname: Wei
keywords: antioxidative enzyme; ethanol; oxidative stress; peroxisome proliferator-activated receptors; resveratrol