title: Comparison between the additive effects of diluted (rFSH) and diluted/dynamized (FSH 6 cH) recombinant follicle-stimulating hormone on the in vitro culture of ovine preantral follicles enclosed in ovarian tissue.
abstract: OBJECTIVE
  This study compared 2 types of recombinant follicle stimulating hormone (rFSH): diluted and diluted/dynamized, on in vitro development of ovine follicles.
  
  
  METHODS
  In experiment 1, ovarian fragments were cultured for 1 or 7 days in α-MEM(+) in the absence or presence of different concentrations of diluted rFSH to determine the best concentration. In experiment 2, the effect of diluted and diluted/dynamized rFSH (rFSH 6 cH--ultradiluted and succussioned), alone or in combination, was studied.
  
  
  RESULTS
  In experiment 1, compared to control, 50ng/mL of diluted rFSH induced higher rates of follicular survival after 7 days of culture and higher percentages of growing follicles at day 1 of culture (P<0.05). In experiment 2, compared to control, diluted/dynamized rFSH induced higher follicular diameter and survival rate after 7 days and early follicle activation at day 1 of culture (P<0.05). Compared to diluted rFSH, diluted/dynamized rFSH induced higher rates of follicle activation at day 1 of culture (P<0.05).
  
  
  CONCLUSIONS
  In conclusion, compared to the control medium, diluted/dynamized rFSH promoted survival and early activation of follicles, while diluted rFSH promoted higher activation later in the culture. Thus, diluted/dynamized rFSH may be used as an alternative to diluted rFSH for the in vitro culture of ovine preantral follicles.
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil. Electronic address: laritza_lima@yahoo.com.br.
authors.forename: L F
authors.initials: LF
authors.lastname: Lima
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: R M P
authors.initials: RM
authors.lastname: Rocha
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: A M C V
authors.initials: AM
authors.lastname: Alves
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: A A
authors.initials: AA
authors.lastname: Carvalho
authors.affiliation: Laboratory Morphofunctional, Health Sciences Center University of Fortaleza, Fortaleza, CE, Brazil.
authors.forename: R N
authors.initials: RN
authors.lastname: Chaves
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: C A P
authors.initials: CA
authors.lastname: Lopes
authors.affiliation: Laboratory of Electron Microscopy, Department of Cell Biology, University of Brasilia, Brasília, DF, Brazil.
authors.forename: S N
authors.initials: SN
authors.lastname: Báo
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: C C
authors.initials: CC
authors.lastname: Campello
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: A P R
authors.initials: AP
authors.lastname: Rodrigues
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: J R
authors.initials: JR
authors.lastname: Figueiredo
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D006080:Ovarian Follicle / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D011994:Recombinant Proteins / Q000494:pharmacology*; D012756:Sheep
chemical_list: D011994:Recombinant Proteins; D005640:Follicle Stimulating Hormone
keywords: Homeopathy; Ovary; Ovine; Reproduction