title: Quantitative blood oxygen saturation imaging using combined photoacoustics and acousto-optics.
abstract: In photoacoustic spectroscopy (PAS), wavelength dependent optical attenuation of biological tissue presents a challenge to measure the absolute oxygen saturation of hemoglobin (sO2). Here, we employ the combination of photoacoustics and acousto-optics (AO) at two optical wavelengths to achieve quantification, where AO serves as a sensor for the relative local fluence. We demonstrate that our method enables compensation of spatial as well as wavelength dependent fluence variations in PAS without a priori knowledge about the optical properties of the medium. The fluence compensated photoacoustic images at two excitation wavelengths are used to estimate the absolute oxygen saturation of blood in a spatially and spectroscopically heterogeneous phantom.
authors.forename: Altaf
authors.initials: A
authors.lastname: Hussain
authors.forename: Wilma
authors.initials: W
authors.lastname: Petersen
authors.forename: Jacob
authors.initials: J
authors.lastname: Staley
authors.forename: Erwin
authors.initials: E
authors.lastname: Hondebrink
authors.forename: Wiendelt
authors.initials: W
authors.lastname: Steenbergen
mesh_terms: D000162:Acoustics; D006801:Humans; D061848:Optical Imaging / Q000379:methods*; D010100:Oxygen / Q000097:blood*; D019047:Phantoms, Imaging; D061088:Photoacoustic Techniques / Q000379:methods*
chemical_list: D010100:Oxygen