title: Conservation of the glycoprotein B homologs of the Kaposi׳s sarcoma-associated herpesvirus (KSHV/HHV8) and old world primate rhadinoviruses of chimpanzees and macaques.
abstract: The envelope-associated glycoprotein B (gB) is highly conserved within the Herpesviridae and plays a critical role in viral entry. We analyzed the evolutionary conservation of sequence and structural motifs within the Kaposi׳s sarcoma-associated herpesvirus (KSHV) gB and homologs of Old World primate rhadinoviruses belonging to the distinct RV1 and RV2 rhadinovirus lineages. In addition to gB homologs of rhadinoviruses infecting the pig-tailed and rhesus macaques, we cloned and sequenced gB homologs of RV1 and RV2 rhadinoviruses infecting chimpanzees. A structural model of the KSHV gB was determined, and functional motifs and sequence variants were mapped to the model structure. Conserved domains and motifs were identified, including an "RGD" motif that plays a critical role in KSHV binding and entry through the cellular integrin αVβ3. The RGD motif was only detected in RV1 rhadinoviruses suggesting an important difference in cell tropism between the two rhadinovirus lineages.
authors.affiliation: Center for Global Infectious Disease Research, Seattle Children׳s Research Institute, Seattle, WA, United States.
authors.forename: A Gregory
authors.initials: AG
authors.lastname: Bruce
authors.affiliation: Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, United States.
authors.forename: Jeremy A
authors.initials: JA
authors.lastname: Horst
authors.affiliation: Center for Global Infectious Disease Research, Seattle Children׳s Research Institute, Seattle, WA, United States; Department of Pediatrics, University of Washington, Seattle, WA, United States. Electronic address: timothy.rose@seattlechildrens.org.
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Rose
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D019143:Evolution, Molecular; D014644:Genetic Variation; D016679:Genome, Viral; D019288:Herpesvirus 8, Human / Q000145:classification / Q000235:genetics*; D006801:Humans; D008253:Macaca mulatta; D008958:Models, Molecular; D002679:Pan troglodytes; D010802:Phylogeny; D011401:Promoter Regions, Genetic; D054730:Protein Interaction Domains and Motifs; D017433:Protein Structure, Secondary; D018144:Rhadinovirus / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013329:Structure-Activity Relationship; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D014759:Viral Envelope Proteins; C116207:glycoprotein B, human herpesvirus 8
keywords: Glycoprotein; Integrin; Kaposi׳s sarcoma herpesvirus; Phylogeny; Retroperitoneal fibromatosis herpesvirus; Rhadinovirus; Structure