title: A Powerful CRISPR/Cas9-Based Method for Targeted Transcriptional Activation.
abstract: Targeted transcriptional activation of endogenous genes is important for understanding physiological transcriptional networks, synthesizing genetic circuits, and inducing cellular phenotype changes. The CRISPR/Cas9 system has great potential to achieve this purpose, however, it has not yet been successfully used to efficiently activate endogenous genes and induce changes in cellular phenotype. A powerful method for transcriptional activation by using CRISPR/Cas9 was developed. Replacement of a methylated promoter with an unmethylated one by CRISPR/Cas9 was sufficient to activate the expression of the neural cell gene OLIG2 and the embryonic stem cell gene NANOG in HEK293T cells. Moreover, CRISPR/Cas9-based OLIG2 activation induced the embryonic carcinoma cell line NTERA-2 to express the neuronal marker βIII-tubulin.
authors.affiliation: Graduate School of Medicine, Hokkaido University, Division of Stem Cell Biology, Institute for Genetic Medicine, Hokkaido University, Sapporo, Hokkaido, 060-0815, Japan. shotakatayama.bio@gmail.com.
authors.forename: Shota
authors.initials: S
authors.lastname: Katayama
authors.affiliation: Graduate School of Medicine, Hokkaido University, Division of Stem Cell Biology, Institute for Genetic Medicine, Hokkaido University, Sapporo, Hokkaido, 060-0815, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Moriguchi
authors.affiliation: Graduate School of Medicine, Hokkaido University, Division of Stem Cell Biology, Institute for Genetic Medicine, Hokkaido University, Sapporo, Hokkaido, 060-0815, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Ohtsu
authors.affiliation: Graduate School of Medicine, Hokkaido University, Division of Stem Cell Biology, Institute for Genetic Medicine, Hokkaido University, Sapporo, Hokkaido, 060-0815, Japan. tkondo@igm.hokudai.ac.jp.
authors.forename: Toru
authors.initials: T
authors.lastname: Kondo
mesh_terms: D064113:CRISPR-Cas Systems / Q000235:genetics*; D002460:Cell Line; D057809:HEK293 Cells; D006801:Humans; D000071317:Nanog Homeobox Protein / Q000235:genetics* / Q000378:metabolism; D000076104:Oligodendrocyte Transcription Factor 2 / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation / Q000235:genetics
chemical_list: C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; C405708:OLIG2 protein, human; D000076104:Oligodendrocyte Transcription Factor 2
keywords: CRISPR/Cas9; epigenetics; gene expression; gene technology; synthetic biology