title: Data set for transcriptional response to depletion of the Shoc2 scaffolding protein.
abstract: The Suppressor of Clear, Caenorhabditis elegans Homolog (SHOC2) is a scaffold protein that positively modulates activity of the RAS/ERK1/2 MAP kinase signaling cascade. We set out to understand the ERK1/2 pathway transcriptional response transduced through the SHOC2 scaffolding module. This data article describes raw gene expression within triplicates of kidney fibroblast-like Cos1 cell line expressing non-targeting shRNA (Cos-NT) and triplicates of Cos1 cells depleted of SHOC2 using shRNA (Cos-LV1) upon activation of ERK1/2 pathway by the Epidermal Growth Factor Receptor (EGFR). The data referred here is available in NCBI׳s Gene Expression Omnibus (GEO), accession GEO: GSE67063 as well as NCBI׳s Sequence Read Archive (SRA), accession SRA: SRP056324. A complete analysis of the results can be found in "Shoc2-tranduced ERK1/2 motility signals - Novel insights from functional genomics"(Jeoung et al., 2016) [1].
authors.affiliation: Department of Computer Engineering and Computer Science, University of Louisville, Louisville, KY 40292, United States; Kentucky Biomedical Research Infrastructure Network Bioinformatics Core, University of Louisville, Louisville, KY 40292, United States.
authors.forename: Eric C
authors.initials: EC
authors.lastname: Rouchka
authors.affiliation: Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40536, United States.
authors.forename: Myoungkun
authors.initials: M
authors.lastname: Jeoung
authors.affiliation: Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40536, United States.
authors.forename: Eun Ryoung
authors.initials: ER
authors.lastname: Jang
authors.affiliation: Markey Cancer Center and Division of Biostatistics, University of Kentucky, Lexington, KY 40536, United States.
authors.forename: Jinpeng
authors.initials: J
authors.lastname: Liu
authors.affiliation: Markey Cancer Center and Division of Biostatistics, University of Kentucky, Lexington, KY 40536, United States.
authors.forename: Chi
authors.initials: C
authors.lastname: Wang
authors.affiliation: Kentucky Biomedical Research Infrastructure Network Bioinformatics Core, University of Louisville, Louisville, KY 40292, United States; Department of Anatomical Sciences and Neurobiology, University of Louisville, Louisville, KY 40292, United States; Department of Bioinformatics and Biostatistics, University of Louisville, Louisville, KY 40292, United States.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40536, United States.
authors.forename: Emilia
authors.initials: E
authors.lastname: Galperin