title: Standard melanoma-associated markers do not identify the MM127 metastatic melanoma cell line.
abstract: Reliable identification of different melanoma cell lines is important for many aspects of melanoma research. Common markers used to identify melanoma cell lines include: S100; HMB-45; and Melan-A. We explore the expression of these three markers in four different melanoma cell lines: WM35; WM793; SK-MEL-28; and MM127. The expression of these markers is examined at both the mRNA and protein level. Our results show that the metastatic cell line, MM127, cannot be detected using any of the commonly used melanoma-associated markers. This implies that it would be very difficult to identify this particular cell line in a heterogeneous sample, and as a result this cell line should be used with care.
authors.affiliation: Institute of Health and Biomedical Innovation, Queensland University of Technology (QUT), Kelvin Grove 4059, Australia.|School of Mathematical Sciences, QUT, PO Box 2434, Brisbane 4001, Australia.
authors.forename: Parvathi
authors.initials: P
authors.lastname: Haridas
authors.affiliation: Institute of Health and Biomedical Innovation, Queensland University of Technology (QUT), Kelvin Grove 4059, Australia.
authors.forename: Jacqui A
authors.initials: JA
authors.lastname: McGovern
authors.affiliation: Institute of Health and Biomedical Innovation, Queensland University of Technology (QUT), Kelvin Grove 4059, Australia.
authors.forename: Abhishek S
authors.initials: AS
authors.lastname: Kashyap
authors.affiliation: Institute of Health and Biomedical Innovation, Queensland University of Technology (QUT), Kelvin Grove 4059, Australia.|School of Mathematical Sciences, QUT, PO Box 2434, Brisbane 4001, Australia.
authors.forename: D L Sean
authors.initials: DL
authors.lastname: McElwain
authors.affiliation: Institute of Health and Biomedical Innovation, Queensland University of Technology (QUT), Kelvin Grove 4059, Australia.|School of Mathematical Sciences, QUT, PO Box 2434, Brisbane 4001, Australia.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Simpson
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism / Q000592:standards*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D006801:Humans; D058965:MART-1 Antigen / Q000235:genetics / Q000378:metabolism; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology; D058950:Melanoma-Specific Antigens / Q000235:genetics / Q000378:metabolism; D009362:Neoplasm Metastasis; D009418:S100 Proteins / Q000235:genetics / Q000378:metabolism; D012680:Sensitivity and Specificity; D058951:gp100 Melanoma Antigen
chemical_list: D014408:Biomarkers, Tumor; D058965:MART-1 Antigen; D058950:Melanoma-Specific Antigens; C548637:PMEL protein, human; D009418:S100 Proteins; D058951:gp100 Melanoma Antigen