title: Effects of Scytosiphon lomentaria on osteoblastic proliferation and differentiation of MC3T3-E1 cells.
abstract: OBJECTIVE
  Bone formation and bone resorption continuously occur in bone tissue to prevent the accumulation of old bone, this being called bone remodeling. Osteoblasts especially play a crucial role in bone formation through the differentiation and proliferation. Therefore, in this study, we investigated the effects of Scytosiphon lomentaria extract (SLE) on osteoblastic proliferation and differentiation in MC3T3-E1 cells.
  
  
  METHODS
  A cell proliferation assay, alkaline phosphatase (ALP) activity assay, alizarin red staining and protein expression analysis of osteoblastic genes were carried out to assess the osteoblastic proliferation and differentiation.
  
  
  RESULTS
  The results indicated that treatment of SLE promoted the proliferation of MC3T3-E1 cells and improved ALP activity. And, SLE treatment significantly promoted mineralized nodule formation compared with control. In addition, cells treated with SLE significantly upregulated protein expression of ALP, type 1 collagen, bone morphogenetic protein 2, runt-related transcription factor 2, osterix, and osteoprotegerin.
  
  
  CONCLUSIONS
  The results demonstrate that SLE promote differentiation inducement and proliferation of osteoblasts and, therefore may help to elucidate the transcriptional mechanism of bone formation and possibly lead to the development of bone-forming drugs.
authors.affiliation: Department of Food and Nutrition, College of Medical and Life Science, Silla University, 140 Baegyang-daero, 700beon-gil, Sasang-Gu, Busan 46958, Korea.
authors.forename: Mi Hwa
authors.initials: MH
authors.lastname: Park
authors.affiliation: Department of Food and Nutrition, College of Medical and Life Science, Silla University, 140 Baegyang-daero, 700beon-gil, Sasang-Gu, Busan 46958, Korea.
authors.forename: Seoyeon
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Food and Nutrition, College of Medical and Life Science, Silla University, 140 Baegyang-daero, 700beon-gil, Sasang-Gu, Busan 46958, Korea.
authors.forename: Jihyeon
authors.initials: J
authors.lastname: Cheon
authors.affiliation: Department of Food and Nutrition, College of Medical and Life Science, Silla University, 140 Baegyang-daero, 700beon-gil, Sasang-Gu, Busan 46958, Korea.
authors.forename: Juyeong
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Food and Nutrition, College of Medical and Life Science, Silla University, 140 Baegyang-daero, 700beon-gil, Sasang-Gu, Busan 46958, Korea.
authors.forename: Bo Kyung
authors.initials: BK
authors.lastname: Kim
authors.affiliation: Department of Pharmaceutical Engineering, College of Medical and Life Science, Silla University, Busan 46958, Korea.
authors.forename: Sang-Hyeon
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Food and Nutrition, College of Medical and Life Science, Silla University, 140 Baegyang-daero, 700beon-gil, Sasang-Gu, Busan 46958, Korea.
authors.forename: Changsuk
authors.initials: C
authors.lastname: Kong
authors.affiliation: ISFOOD Co. LTD., 7, Hoenggye-gil, Ilgwang-myeon, Gijang-gun, Busan 46048, Korea.
authors.forename: Yuck Yong
authors.initials: YY
authors.lastname: Kim
authors.affiliation: Department of Food and Nutrition, College of Medical and Life Science, Silla University, 140 Baegyang-daero, 700beon-gil, Sasang-Gu, Busan 46958, Korea.
authors.forename: Mihyang
authors.initials: M
authors.lastname: Kim
keywords: MC3T3-E1 cell; Scytosiphon lomentaria; alkaline phosphatase; differentiation; mineralization