title: [Expression of LRG-1 in clinical specimens and Tca8113 cell line of tongue carcinoma].
abstract: OBJECTIVE
  To investigate the expression of LRG-1 in clinical specimens and Tca8113 cell line of tongue carcinoma and analyze the relationship between LRG-1 expression and the clinicopathological parameters.
  
  
  METHODS
  LRG-1 expression was detected in 40 tongue squamous cell carcinoma (TSCC) tissues and paired normal adjacent tissues, 20 atypical hyperplasia tissues of the tongue, and 20 tissues of tongue cancer in situ using immunohistochemical method. The expression of LRG-1 in Tca8113 cell line was detected using flow cytometry. The expression of LRG-1 was also detected in human TSCC tissues and Tca8113 cells with Western blotting. The effect of LRG-1 on the proliferation of HUVECs was determined using MTT assay, and its effect on angiogenesis was evaluated with Matrigel tube formation assays.
  
  
  RESULTS
  Human TSCC tissues had a significantly higher rate of positive expression for LRG-1 (85%, 34/40) than the adjacent tissues (10%, 4/40), invasive tongue cancer (30%, 6/20), and tongue cancer in situ (50%, 10/20) (P<0.05). LRG-1 expression was correlated with the degree of tumor differentiation, clinical stage and lymph node metastasis of the tumor (P<0.05) but not with the patients' age or gender. In the in vitro experiment, LRG-1 promoted HUVEC proliferation and angiogenesis.
  
  
  CONCLUSIONS
  Abnormal LRG-1 expression is present in the human TSCC tissue and Tca8113 cells. LRG-1 can promote HUVEC proliferation and angiogenesis in vitro, suggesting its possible role in promoting tumor angiogenesis.
authors.affiliation: Department of Dentistry, First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, China.E-mail: 1028548274@qq.com.
authors.forename: Li-Jing
authors.initials: LJ
authors.lastname: Hao
authors.forename: Wen-Jiao
authors.initials: WJ
authors.lastname: Zheng
authors.forename: Shu-Fen
authors.initials: SF
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zheng
authors.forename: Shao-Heng
authors.initials: SH
authors.lastname: He
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008207:Lymphatic Metastasis; D014059:Tongue / Q000378:metabolism / Q000473:pathology; D014062:Tongue Neoplasms / Q000235:genetics / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; C015246:LRG1 protein, human