title: Mechanism study of PEGylated polyester and β-cyclodextrin integrated micelles on drug resistance reversal in MRP1-overexpressed HL60/ADR cells.
abstract: Chemotherapy is one of the main strategies for cancer treatment, but its effective application is seriously limited by the development of drug resistance. In this study, we designed micellar vectors for doxorubicin based on amphiphilic copolymers sequentially linking β-cyclodextrin (β-CD), polylacticacid (PLA) or polycaprolactone (PCL) block, and polyethylene glycol (PEG) block to overcome drug resistance in human acute myeloid leukemia cells (HL60/ADR) overexpressing multidrug resistance protein 1 (MRP1). The significant enhancement in cytotoxicity and inhibited HL60/ADR tumor growth in mouse was achieved. More importantly, several analyses were performed to understand the interactions between various polymers and MRP1 at the cellular level. The results showed that the polymers did not show remarkable correlation of MRP1 gene and protein expression, but could decrease intracellular ATP, mitochondrial membrane potential and glutathione levels, which was greatly dependent on the molecular structure of polymers. In conclusion, these novel micelles can be considered as a kind of promising drug delivery system for tumor therapy to reverse drug resistance related to MRP1 overexpression.
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, 866 Yu-Hang-Tang Road, Hangzhou 310058, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Ji
authors.affiliation: Ministry of Education (MOE) Key Laboratory of Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, 38 Zheda Road, Hangzhou 310027, China. Electronic address: lyqiu@zju.edu.cn.
authors.forename: Liyan
authors.initials: L
authors.lastname: Qiu
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D004705:Endocytosis / Q000187:drug effects; D005260:Female; D005434:Flow Cytometry; D005452:Fluoresceins / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D042541:Intracellular Space / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008823:Micelles; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000378:metabolism*; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000255:Adenosine Triphosphate; D004317:Doxorubicin; D005452:Fluoresceins; D005978:Glutathione; D008823:Micelles; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D011091:Polyesters; D011092:Polyethylene Glycols; D047392:beta-Cyclodextrins; C085925:calcein AM; C031215:betadex; C421109:multidrug resistance-associated protein 1
keywords: Acute myeloid leukemia cells; Doxorubicin; Micelle; Multidrug resistance; Multidrug resistance protein 1