title: TiLIA: a software package for image analysis of firefly flash patterns.
abstract: As flash signaling patterns of fireflies are species specific, signal-pattern analysis is important for understanding this system of communication. Here, we present time-lapse image analysis (TiLIA), a free open-source software package for signal and flight pattern analyses of fireflies that uses video-recorded image data. TiLIA enables flight path tracing of individual fireflies and provides frame-by-frame coordinates and light intensity data. As an example of TiLIA capabilities, we demonstrate flash pattern analysis of the fireflies Luciola cruciata and L. lateralis during courtship behavior.
authors.affiliation: Advanced Analysis Technology Department Olympus Corporation Kuboyama 2-3 Hachioji Tokyo 192-8512 Japan.
authors.forename: Junsuke
authors.initials: J
authors.lastname: Konno
authors.affiliation: Advanced Analysis Technology Department Olympus Corporation Kuboyama 2-3 Hachioji Tokyo 192-8512 Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Hatta-Ohashi
authors.affiliation: Advanced Analysis Technology Department Olympus Corporation Kuboyama 2-3 Hachioji Tokyo 192-8512 Japan.
authors.forename: Ryutaro
authors.initials: R
authors.lastname: Akiyoshi
authors.affiliation: Department of Entomology Faculty of Agriculture Kasetsart University Chatuchak Bangkok 10900 Thailand.
authors.forename: Anchana
authors.initials: A
authors.lastname: Thancharoen
authors.affiliation: Entomology Section Queen Sirikit Botanic Garden Maerim Chiang Mai 50180 Thailand.
authors.forename: Somyot
authors.initials: S
authors.lastname: Silalom
authors.affiliation: Department of National Parks, Wildlife and Plant Conservation Forest and Plant Conservation Research Office Chatuchak Bangkok 10900 Thailand.
authors.forename: Watana
authors.initials: W
authors.lastname: Sakchoowong
authors.affiliation: Hong Kong Entomological Society Yuen Long, Hong Kong China.
authors.forename: Vor
authors.initials: V
authors.lastname: Yiu
authors.affiliation: The Ohba Firefly Institute Mabori-kaigan 4-1-12-204 Yokosuka Kanagawa 239-0801 Japan.
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Ohba
authors.affiliation: Advanced Analysis Technology Department Olympus Corporation Kuboyama 2-3 Hachioji Tokyo 192-8512 Japan.
authors.forename: Hirobumi
authors.initials: H
authors.lastname: Suzuki
keywords: Flash communication; flight path tracing; time‐lapse image analysis