title: Recombinant Mycobacterium bovis bacillus Calmette-Guérin expressing Ag85B-IL-7 fusion protein enhances IL-17A-producing innate γδ T cells.
abstract: Interleukin 7 (IL-7) has an important function in the development and maintenance of IL-17A+ γδ T cells. We here constructed a recombinant Mycobacterium bovis bacillus Calmette-Guérin expressing antigen 85B (Ag85B)-IL-7 fusion protein (rBCG-Ag85B-IL-7). The Ag85B-IL-7 fusion protein and IL-7 were detected in the bacterial lysate of rBCG-Ag85B-IL-7. rBCG-Ag85B-IL-7 was the same in number as control rBCG expressing Ag85B (rBCG-Ag85B) in the lung at the early stage after intravenous inoculation, whereas the numbers of IL-17A+ γδ T cells and Ag-specific Th1 cells were significantly higher in the lungs of mice inoculated with rBCG-Ag85B-IL-7 than those inoculated with rBCG-Ag85B. The Ag-specific Th1 cell response was impaired in mice lacking IL-17A+ γδ T cells after inoculation with rBCG-Ag85B-IL-7. Thus, rBCG-Ag85B-IL-7 increases the pool size of IL-17A+ γδ T cells, which subsequently augment the Th1 response to mycobacterial infection.
authors.affiliation: Division of Host Defense, Medical Institute of Bioregulation, Kyushu University, 3-1-1, Maidaishi, Higashi-ku, Fukuoka 812-8582, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Hatano
authors.affiliation: Department of Microbiology, Leprosy Research Center, National Institute of Infectious Diseases, Tokyo 189-0002, Japan.
authors.forename: Toshiki
authors.initials: T
authors.lastname: Tamura
authors.affiliation: Molecular Microbiology Group, Department of Tropical Infectious Diseases, Center of Molecular Biosciences, Tropical Biosphere Research Center, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Umemura
authors.affiliation: Molecular Microbiology Group, Department of Tropical Infectious Diseases, Center of Molecular Biosciences, Tropical Biosphere Research Center, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan.
authors.forename: Goro
authors.initials: G
authors.lastname: Matsuzaki
authors.affiliation: Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1, Shikata-cho, Kita-ku, Okayama 700-8525, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Ohara
authors.affiliation: Division of Host Defense, Medical Institute of Bioregulation, Kyushu University, 3-1-1, Maidaishi, Higashi-ku, Fukuoka 812-8582, Japan. Electronic address: yoshikai@bioreg.kyushu-u.ac.jp.
authors.forename: Yasunobu
authors.initials: Y
authors.lastname: Yoshikai
mesh_terms: D000217:Acyltransferases / Q000235:genetics* / Q000276:immunology*; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology*; D001500:BCG Vaccine / Q000235:genetics / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology*; D016207:Cytokines / Q000276:immunology; D007113:Immunity, Innate; D020381:Interleukin-17 / Q000276:immunology*; D015851:Interleukin-7 / Q000235:genetics* / Q000276:immunology; D008168:Lung / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D009163:Mycobacterium bovis / Q000235:genetics* / Q000254:growth & development / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D001500:BCG Vaccine; D001426:Bacterial Proteins; D016207:Cytokines; D020381:Interleukin-17; D015851:Interleukin-7; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011993:Recombinant Fusion Proteins; D000217:Acyltransferases; C121433:antigen 85B, Mycobacterium tuberculosis
keywords: BCG; Bacteria; Cytokines; IL-17A; IL-7; γδ T cells