title: [Effect of F10 gene silencing and over-expression on cell cycle of choriocarcinoma cell line JAR and the mechanisms].
abstract: OBJECTIVE
  To explore the role of F10 gene in regulating cell cycles of choriocarcinoma cells and the underlying mechanisms.
  
  
  METHODS
  Using untreated cells as the control, JAR cells with F10 gene silencing or stable F10 over-expression were examined for cell cycle changes by flow cytometry (FCM) and for expressions of cyclin and cyclin-dependent kinase (CDKs) with Western blotting and immunofluorescence technique.
  
  
  RESULTS
  JAR cells over-expressing F10 gene showed reduced duration of cell cycle compared with untreated and with cells after F10 gene silencing. In F10-over-expressing cells, Western blotting revealed significantly up-regulated expressions of cyclin A2, B1, D1, E and CDK2, 6, and 7, but not CDK4, as compared with the control cells and cells with F10 gene silencing (P<0.05), and these results were consistent with those by immunofluorescence assay.
  
  
  CONCLUSIONS
  F10 gene may accelerate cell cycle progression and promote cell proliferation by up-regulating the expressions of cyclin A2, B1, D1, E and CDK 2, 4, 6, 7 in choriocarcinoma cells.
authors.affiliation: Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.E-mail: 1017692193@qq.com.
authors.forename: Jin-di
authors.initials: JD
authors.lastname: Yang
authors.forename: Zhan-Jun
authors.initials: ZJ
authors.lastname: Pang
mesh_terms: D002453:Cell Cycle; D002455:Cell Division; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002822:Choriocarcinoma / Q000378:metabolism*; D018844:Cyclin-Dependent Kinases / Q000378:metabolism; D016213:Cyclins / Q000378:metabolism; D005170:Factor X / Q000235:genetics*; D005260:Female; D020868:Gene Silencing; D006801:Humans; D011247:Pregnancy
chemical_list: D016213:Cyclins; D005170:Factor X; D018844:Cyclin-Dependent Kinases