title: Water-insoluble thin films from palmitoyl hyaluronan with tunable properties.
abstract: Hyaluronan (HA) films exhibit properties suitable for various biomedical applications, but the solubility of HA limits their use in aqueous environments. Therefore, we developed water insoluble films based on palmitoyl esters of HA (pHA). Films were prepared from pHA samples with various degrees of substitution (DS) and molecular weights and their mechanical properties and swelling were characterized. Additionally, scanning electron microscopy and atomic force microscopy were used for visualization. Despite being prepared by solution casting, the films had a very smooth surface and were homogeneous in thickness. The film properties were in accordance with the polymer DS and molecular weight, enabling to tailor them for future applications by choosing a suitable pHA material. The behavior of the films toward cells was assessed in vitro. All films were non-cytotoxic and showed no adhesion of cells. These results show that the developed films are suitable candidates for various biomedical applications such as tissue engineering or wound healing.
authors.affiliation: Contipro Biotech s.r.o., Dolní Dobrouč 401, Dolní Dobrouč 561 02, Czech Republic. Electronic address: Marcela.Foglarova@contipro.com.
authors.forename: Marcela
authors.initials: M
authors.lastname: Foglarová
authors.affiliation: Contipro Biotech s.r.o., Dolní Dobrouč 401, Dolní Dobrouč 561 02, Czech Republic.
authors.forename: Josef
authors.initials: J
authors.lastname: Chmelař
authors.affiliation: Contipro Pharma a.s., Dolní Dobrouč 401, Dolní Dobrouč 561 02, Czech Republic.
authors.forename: Gloria
authors.initials: G
authors.lastname: Huerta-Angeles
authors.affiliation: Contipro Pharma a.s., Dolní Dobrouč 401, Dolní Dobrouč 561 02, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Vágnerová
authors.affiliation: Contipro Pharma a.s., Dolní Dobrouč 401, Dolní Dobrouč 561 02, Czech Republic.
authors.forename: Jaromír
authors.initials: J
authors.lastname: Kulhánek
authors.affiliation: Department of Medical Biophysics, Faculty of Medicine and Dentistry, Institute of Molecular and Translational Medicine, Palacky University, Hnĕvotínská 3, Olomouc 775 15, Czech Republic.
authors.forename: Kateřina
authors.initials: K
authors.lastname: Bartoň Tománková
authors.affiliation: Centre of Polymer Systems, Tomas Bata University in Zlín, Třída Tomáše Bati 5678, Zlín 760 01, Czech Republic.
authors.forename: Antonín
authors.initials: A
authors.lastname: Minařík
authors.affiliation: Contipro Biotech s.r.o., Dolní Dobrouč 401, Dolní Dobrouč 561 02, Czech Republic.
authors.forename: Vladimír
authors.initials: V
authors.lastname: Velebný
mesh_terms: D000215:Acylation; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D006801:Humans; D006820:Hyaluronic Acid / Q000031:analogs & derivatives / Q000737:chemistry* / Q000633:toxicity; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D010168:Palmitates / Q000737:chemistry* / Q000633:toxicity; D012995:Solubility; D041701:Swiss 3T3 Cells; D013718:Tensile Strength; D014867:Water / Q000737:chemistry
chemical_list: D010168:Palmitates; D014867:Water; D006820:Hyaluronic Acid
keywords: Palmitoyl hyaluronan; Solution casting; Swelling; Tensile properties; Thin film