title: Graphene based aptasensor for glycated albumin in diabetes mellitus diagnosis and monitoring.
abstract: We selected and modified DNA aptamers specifically bound glycated human serum albumin (GHSA), which is an intermediate marker for diabetes mellitus. Our aptamer truncation study indicated that the hairpin-loop structure with 23 nucleotides length containing triple G-C hairpins and 15-nucleotide loop, plays an important role in GHSA binding. Fluorescent quenching graphene oxide (GO) and Cy5-labeled G8 aptamer were used in this study to develop simple and sensitive graphene based aptasensor for GHSA detection. The limit of detection (LOD) of our aptasensor was 50 μg/mL, which was lower than other existing methods. In addition, with the nuclease resistance system, our GHSA detection platform could also be used in clinical samples. Importantly, our approach could significantly reveal the higher levels of GHSA concentrations in diabetes than normal serums. These indicate that our aptasensor has a potential for diagnosis and monitoring of diabetes mellitus.
authors.affiliation: National Nanotechnology Center, National Science and Technology Development Agency, Thailand Science Park, Pathumthani, Thailand.
authors.forename: Chayachon
authors.initials: C
authors.lastname: Apiwat
authors.affiliation: Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors.forename: Patraporn
authors.initials: P
authors.lastname: Luksirikul
authors.affiliation: Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors.forename: Pacharapon
authors.initials: P
authors.lastname: Kankla
authors.affiliation: Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand; Center for Advanced Studies in Nanotechnology and Its Applications in Chemical, Food and Agricultural Industries, Kasetsart University, Bangkok 10900, Thailand.
authors.forename: Prapasiri
authors.initials: P
authors.lastname: Pongprayoon
authors.affiliation: National Nanotechnology Center, National Science and Technology Development Agency, Thailand Science Park, Pathumthani, Thailand.
authors.forename: Kiatnida
authors.initials: K
authors.lastname: Treerattrakoon
authors.affiliation: Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.
authors.forename: Kittiphong
authors.initials: K
authors.lastname: Paiboonsukwong
authors.affiliation: Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.
authors.forename: Suthat
authors.initials: S
authors.lastname: Fucharoen
authors.affiliation: National Nanotechnology Center, National Science and Technology Development Agency, Thailand Science Park, Pathumthani, Thailand; Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Tararaj
authors.initials: T
authors.lastname: Dharakul
authors.affiliation: National Nanotechnology Center, National Science and Technology Development Agency, Thailand Science Park, Pathumthani, Thailand. Electronic address: deanpen@nanotec.or.th.
authors.forename: Deanpen
authors.initials: D
authors.lastname: Japrung
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D002232:Carbocyanines / Q000737:chemistry; D003920:Diabetes Mellitus / Q000097:blood / Q000175:diagnosis*; D005456:Fluorescent Dyes / Q000737:chemistry; D017127:Glycation End Products, Advanced; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D010087:Oxides / Q000737:chemistry; D012709:Serum Albumin / Q000032:analysis*; D000093423:Glycated Serum Albumin
chemical_list: D052157:Aptamers, Nucleotide; D002232:Carbocyanines; D005456:Fluorescent Dyes; D017127:Glycation End Products, Advanced; D010087:Oxides; D012709:Serum Albumin; C085321:cyanine dye 5; D006108:Graphite; D000093423:Glycated Serum Albumin
keywords: Aptamer; Aptasensor; Diabetes mellitus; Glycated human serum albumin; Graphene oxide