title: Molecular Mycobacteriology and Expansion in Disease Diagnosis.
abstract: Molecular diagnostic tools for tuberculosis (TB) have evolved quickly with new innovations which can provide unprecedented opportunities for the rapid, sensitive and specific diagnosis of M. tuberculosis in clinical specimens and the status of its drug sensitivity. Microscopy and culture methods can not be replaced but the molecular assays can be applied in parallel with any new molecular tests for the diagnosis of TB. For extra pulmonary specimens, the use of the amplification methods is advocated, since rapid and accurate laboratory diagnosis is critical. Customization of the diagnostic usefulness of a molecular assay, according to the ease, reliability and need for health care sector is of immense value in a modern clinical mycobacteriology laboratory.
authors.affiliation: Central Molecular Research Laboratory, Department of Biochemistry, SGRR Institute of Medical & Health Sciences, Dehradun, India.
authors.forename: Narotam
authors.initials: N
authors.lastname: Sharma
authors.affiliation: Central Molecular Research Laboratory, Department of Biochemistry, SGRR Institute of Medical & Health Sciences, Dehradun, India.
authors.forename: R K
authors.initials: RK
authors.lastname: Singh
authors.affiliation: Biochemistry Department, All India Institute of Medical Sciences, Jodhpur, Rajasthan India.
authors.forename: Praveen
authors.initials: P
authors.lastname: Sharma
keywords: Amplification; Master mix; Multiple drug resistance; Nested PCR