title: Domibacillus antri sp. nov., isolated from the soil of a cave.
abstract: A Gram-reaction-positive, strictly aerobic, capsule-forming, motile and rod-shaped bacterium, designated strain XD80T, was isolated from the soil of a native cave in Lichuan, Hubei province, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain XD80Twas most closely related to Domibacillus iocasae CCTCC AB 2015183T(98.66 % sequence similarity), followed by Domibacillus robiginosus DSM 25058T(97.83 %), Domibacillus tundrae KCTC 33549T(97.70 %), Domibacillus enclensis CCTCC AB 2011121T(97.21 %) and Domibacillus indicus DSM 28032T(96.96 %). Levels of DNA-DNA relatedness between strain XD80Tand D. iocasae CCTCC AB 2015183T, D. robiginosusDSM 25058T, D. tundrae KCTC 33549Tand D. enclensis CCTCC AB 2011121Twere 37.4 %, 53.8 %, 53.6 % and 52.7 %, respectively. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, aminophospholipid and two unknown phospholipids. The predominant fatty acids (>5 %) were iso-C15 : 0 (37.3 %), anteiso-C15 : 0 (10.8 %), C16 : 0 (10.4 %), iso-C17 : 0 (10.3 %), C16 : 1ω11c (9.6 %) and anteiso-C17 : 0 (7.4 %). MK-6 (86.4 %) was the major respiratory quinone. The DNA G+C content was 46.4 mol%. The cell-wall peptidoglycan contained meso-diaminopimelic acid (type A1γ). Ribose and glucose were the major whole-cell sugars. In addition, strain XD80Tshowed differential physiological characteristics from most members of the genus Domibacillus, encompassing hydrolysis of starch, acid production from inositol and raffinose, and production of valine arylamidase. The results of this polyphasic study indicated that strain XD80Trepresents a novel species of the genus Domibacillus, for which the name Domibacillus antri sp. nov. is proposed. The type strain is XD80T(=CCTCC AB 2015053T=KCTC 33636T).
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, PR China.
authors.forename: Ding
authors.initials: D
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, PR China.
authors.forename: Libing
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, PR China.
authors.forename: Gejiao
authors.initials: G
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, PR China.
authors.forename: Shixue
authors.initials: S
authors.lastname: Zheng
mesh_terms: D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D059552:Caves / Q000382:microbiology*; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C042455:menaquinone 6
keywords: Domibacillus soil Firmicutes Polyphasic identification