title: Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues.
abstract: Obesity is an unconstrained worldwide epidemic. Unraveling molecular controls in adipose tissue development holds promise to treat obesity or diabetes. Although numerous immortalized adipogenic cell lines have been established, adipose-derived stem cells from the stromal vascular fraction of subcutaneous white adipose tissues provide a reliable cellular system ex vivo much closer to adipose development in vivo. Pig adipose-derived stem cells (pADSC) are isolated from 7- to 9-day old piglets. The dorsal white fat depot of porcine subcutaneous adipose tissues is sliced, minced and collagenase digested. These pADSC exhibit strong potential to differentiate into adipocytes. Moreover, the pADSC also possess multipotency, assessed by selective stem cell markers, to differentiate into various mesenchymal cell types including adipocytes, osteocytes, and chondrocytes. These pADSC can be used for clarification of molecular switches in regulating classical adipocyte differentiation or in direction to other mesenchymal cell types of mesodermal origin. Furthermore, extended lineages into cells of ectodermal and endodermal origin have recently been achieved. Therefore, pADSC derived in this protocol provide an abundant and assessable source of adult mesenchymal stem cells with full multipotency for studying adipose development and application to tissue engineering of regenerative medicine.
authors.affiliation: Institute of Biotechnology, National Taiwan University; Department of Animal Science and Technology, National Taiwan University.
authors.forename: Yu-Jen
authors.initials: YJ
authors.lastname: Chen
authors.affiliation: Department of Animal Science and Technology, National Taiwan University.
authors.forename: Hui-Yu
authors.initials: HY
authors.lastname: Liu
authors.affiliation: Department of Animal Science and Technology, National Taiwan University.
authors.forename: Yun-Tsui
authors.initials: YT
authors.lastname: Chang
authors.affiliation: Department of Animal Science and Technology, National Taiwan University.
authors.forename: Ying-Hung
authors.initials: YH
authors.lastname: Cheng
authors.affiliation: Department of Animal Science and Technology, National Taiwan University.
authors.forename: Harry J
authors.initials: HJ
authors.lastname: Mersmann
authors.affiliation: Department of Surgery, National Taiwan University Hospital and College of Medicine.
authors.forename: Wen-Hung
authors.initials: WH
authors.lastname: Kuo
authors.affiliation: Institute of Biotechnology, National Taiwan University; Department of Animal Science and Technology, National Taiwan University; sding@ntu.edu.tw.
authors.forename: Shih-Torng
authors.initials: ST
authors.lastname: Ding
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002469:Cell Separation; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D044968:Regenerative Medicine / Q000379:methods; D013552:Swine; D023822:Tissue Engineering / Q000379:methods