title: Effects of risedronate on osteoblastic cell cultures.
abstract: OBJECTIVE
  Bisphosphonates (BPs) have been widely used in the treatment of bone disorders due to their ability to modulate bone turnover. The biological mechanisms through BFs exert their effects on osteoclasts are well established. However, the role of BFs on the osteoblasts is controversial. The present study aimed to evaluate the effects of risedronate on osteoblastic cells.
  
  
  METHODS
  MC3TE-E1 cells were exposed to risedronate at 0, 10(-8), 10(-6), 10(-4), and 10(-3)M. The following parameters were assayed: (1) cell proliferation by hemocytometer counting after 24, 48 and 72h, (2) cell viability by MTT assay after 24, 48 and 72h, (3) Type I Collagen quantification by ELISA after 24, 48 and 72h, (3) alkaline phosphatase activity after 7 and 10days and (4) matrix mineralization after 14days.
  
  
  RESULTS
  After 24h, risedronate did not affect both cell proliferation and viability (p>0.05). However, after 48 and 72h, a decrease in cell proliferation and viability was detected in osteoblastic cultures exposed to risedronate at 10(-4) and 10(-3)M (p<0.05). After 48 and 72h, Type I Collagen synthesis was stimulated by risedronate at 10(-4)M (p<0.05). High levels of ALP activity were detected in cultures exposed to risedronate at 10(-4)M after 7 and 10days (p<0.05). After 14day, high calcium content was observed in cultures exposed to risedronate at 10(-4)M (p>0.05).
  
  
  CONCLUSIONS
  These results indicated that risedronate can promote osteoblast differentiation.
authors.affiliation: Laboratory of Immunology and Molecular Biology, São Leopoldo Mandic Institute and Research Center, Campinas, SP, Brazil.
authors.forename: Maurilio
authors.initials: M
authors.lastname: Malavasi
authors.affiliation: Laboratory of Immunology and Molecular Biology, São Leopoldo Mandic Institute and Research Center, Campinas, SP, Brazil.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Louro
authors.affiliation: Laboratory of Immunology and Molecular Biology, São Leopoldo Mandic Institute and Research Center, Campinas, SP, Brazil.
authors.forename: Marcelo Barbosa
authors.initials: MB
authors.lastname: Barros
authors.affiliation: Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Campinas, SP, Brazil.
authors.forename: Lucas Novaes
authors.initials: LN
authors.lastname: Teixeira
authors.affiliation: Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Campinas, SP, Brazil.
authors.forename: Daiane Cristina
authors.initials: DC
authors.lastname: Peruzzo
authors.affiliation: Laboratory of Immunology and Molecular Biology, São Leopoldo Mandic Institute and Research Center, Campinas, SP, Brazil.
authors.forename: Júlio César
authors.initials: JC
authors.lastname: Joly
authors.affiliation: Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Campinas, SP, Brazil.
authors.forename: Elizabeth Ferreira
authors.initials: EF
authors.lastname: Martinez
authors.affiliation: Laboratory of Immunology and Molecular Biology, São Leopoldo Mandic Institute and Research Center, Campinas, SP, Brazil. Electronic address: napimogamh@yahoo.com.
authors.forename: Marcelo Henrique
authors.initials: MH
authors.lastname: Napimoga
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects; D000068296:Risedronic Acid / Q000494:pharmacology*
chemical_list: D024042:Collagen Type I; D000469:Alkaline Phosphatase; D000068296:Risedronic Acid; D002118:Calcium
keywords: Bisphosphonate; Collagen; Mineralization; Osteoblast