title: Antimalarial properties of crude extracts of seeds of Brucea antidysenterica and leaves of Ocimum lamiifolium.
abstract: BACKGROUND
  The search for new antimalarial drugs has become increasingly urgent due to plasmodial resistance to existing drugs. As part of this global effort, the present study aimed at evaluating the antimalarial activity of two traditionally used medicinal plants against the disease.
  
  
  METHODS
  Acute toxicity and four-day suppressive effects of aqueous, methanol and chloroform extracts of the seed and leaf of Brucea antidysenterica and Ocimum lamiifolium, respectively, were investigated in Swiss albino mice using Plasmodium berghei using standard procedures.
  
  
  RESULTS
  Methanol extract of the leaves of O. lamiifolium did not exhibit any sign of acute toxicity up to the dose of 2000 mg/kg body weight. However, all mice provided with seeds of B. antidesenterica at a dose of 2000 mg/kg body died within 24 h. The aqueous, methanol and chloroform crude extracts of B. antidesenterica significantly (p < 0.05) inhibited parasitaemia in a dose-dependent manner and prevented body weight loss at doses of 200, 400 and 600 mg/kg body weight. In addition, the extracts prolonged the mean survival time of P. berghei-infected mice compared to the non-treated control. However, it did not prevent reduction in packed cell volume except the chloroform extract in three doses and methanol extract at 200 mg/kg and 400 mg/kg. Extracts from O. lamiifolium also exhibited significant (p < 0.05) antiplasmodial activities. The extracts did not prevent body weight loss and PCV reduction, especially in chloroform. The highest suppression was recorded from aqueous crude extract of O. lamiifolium with 35.53 % in the dose of 600 mg/kg. On the other hand, a similar higher suppression was found in both methanol and chloroform of crude extracts of B. antidesenterica with 47.70 %, 46.44 % of chemosuppression, respectively, in its highest dose tested.
  
  
  CONCLUSIONS
  Crude aqueous, methanol and chloroform extracts of the two medicinal plants possess acceptable antimalarial effects. However, further investigation should be pursued on toxicity study and to isolate the bioactive components responsible for the observed antimalarial action of the plants.
authors.affiliation: Department of Microbial, Cellular and Molecular Biology, College of Natural Sciences, Addis Ababa University, P.O.Box 1176, Addis Ababa, Ethiopia.
authors.forename: Atetetgeb
authors.initials: A
authors.lastname: Kefe
authors.affiliation: Aklilu Lemma Institute of Pathobiology, Addis Ababa University, P. O. Box 1176, Addis Ababa, Ethiopia. mirutseg@yahoo.com.
authors.forename: Mirutse
authors.initials: M
authors.lastname: Giday
authors.affiliation: Department of Microbial, Cellular and Molecular Biology, College of Natural Sciences, Addis Ababa University, P.O.Box 1176, Addis Ababa, Ethiopia.
authors.forename: Hassen
authors.initials: H
authors.lastname: Mamo
authors.affiliation: Aklilu Lemma Institute of Pathobiology, Addis Ababa University, P. O. Box 1176, Addis Ababa, Ethiopia.
authors.forename: Berhanu
authors.initials: B
authors.lastname: Erko
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D032269:Brucea / Q000737:chemistry*; D005260:Female; D008288:Malaria / Q000188:drug therapy* / Q000469:parasitology; D008297:Male; D051379:Mice; D031339:Ocimum / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology* / Q000633:toxicity; D018515:Plant Leaves / Q000737:chemistry; D010962:Plasmodium berghei / Q000187:drug effects; D012639:Seeds / Q000737:chemistry
chemical_list: D000962:Antimalarials; D010936:Plant Extracts
keywords: Brucea antidysenterica; Ethiopia; Malaria; Ocimum lamiifolium; Plasmodium berghei