title: Multi-residue method for the determination of organofluorine pesticides in fish tissue by liquid chromatography triple quadrupole tandem mass spectrometry.
abstract: A multiresidue method for the determination of organofluorine pesticides in fish tissue samples was developed and optimized. This method is based on a cleanup step of the extracted samples with acetonitrile, and carried out by solid phase extraction (SPE) with aminopropyl (NH2) cartridge, before the identification and quantification of the residues by liquid chromatography triple quadrupole tandem mass spectrometry (LC-MS/MS). The performance characteristics, such as accuracy, precision, linear range, limits of detection (LOD) and quantification (LOQ), for each pesticide were determined. The proposed method allowed high recoveries (80.4-99.2%) of spiked extracted fat samples at 0.5-10 ng g(-1), and very low LODs (between 0.10 and 0.15 ng g(-1)) and LOQs (between 0.3 and 0.5 ng g(-1)) determined in the fish samples. The practicality and high sensitivity of this method have been demonstrated in the determination of residues of OFPs in carp fish fed in water containing 10 ng mL(-1) OFPs.
authors.affiliation: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, Hubei 430223, PR China; School of Resource and Environmental Sciences, Wuhan University, Wuhan, Hubei 430079, PR China; Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan 430070, PR China. Electronic address: gjh@yfi.ac.cn.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Gan
authors.affiliation: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, Hubei 430223, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Lv
authors.affiliation: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, Hubei 430223, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Peng
authors.affiliation: School of Environmental Engineering, Wuhan Textile University, Wuhan, Hubei 430200, PR China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Resource and Environmental Sciences, Wuhan University, Wuhan, Hubei 430079, PR China.
authors.forename: Zhiting
authors.initials: Z
authors.lastname: Xiong
authors.affiliation: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, Hubei 430223, PR China.
authors.forename: Daqing
authors.initials: D
authors.lastname: Chen
authors.affiliation: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, Hubei 430223, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: He
mesh_terms: D000097:Acetonitriles; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005399:Fishes; D010575:Pesticides / Q000032:analysis*; D017747:Seafood / Q000032:analysis*; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000097:Acetonitriles; D010575:Pesticides; C032159:acetonitrile
keywords: Fish; LC–MS/MS; Organofluorine pesticides; Solid phase extraction (SPE)