title: An Approach to Identify and Characterize a Subunit Candidate Shigella Vaccine Antigen.
abstract: Shigellosis remains a serious issue throughout the developing countries, particularly in children under the age of 5. Numerous strategies have been tested to develop vaccines targeting shigellosis; unfortunately despite several years of extensive research, no safe, effective, and inexpensive vaccine against shigellosis is available so far. Here, we illustrate in detail an approach to identify and establish immunogenic outer membrane proteins from Shigella flexneri 2a as subunit vaccine candidates.
authors.affiliation: Department of Immunology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, 44195, USA.
authors.forename: Debasis
authors.initials: D
authors.lastname: Pore
authors.affiliation: Division of Pathophysiology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road, Scheme-XM, Beliaghata, Kolkata, 700010, West Bengal, India. mkc_niced@yahoo.co.in.
authors.forename: Manoj K
authors.initials: MK
authors.lastname: Chakrabarti
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D003001:Cloning, Molecular; D005260:Female; D015870:Gene Expression; D008213:Lymphocyte Activation / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000276:immunology; D008297:Male; D051379:Mice; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D017421:Sequence Analysis; D012760:Shigella / Q000276:immunology*; D022281:Shigella Vaccines / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D022223:Vaccines, Subunit / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D011994:Recombinant Proteins; D022281:Shigella Vaccines; D022223:Vaccines, Subunit
keywords: B cell; Macrophages; Outer membrane proteins; Protective immune response; Shigella flexneri 2a; T cell