title: Transcriptome Analysis Revealed Changes of Multiple Genes Involved in Haliotis discus hannai Innate Immunity during Vibrio parahemolyticus Infection.
abstract: Abalone (Haliotis discus hannai) is one of the most valuable marine aquatic species in Korea, Japan and China. Tremendous exposure to bacterial infection is common in aquaculture environment, especially by Vibrio sp. infections. It's therefore necessary and urgent to understand the mechanism of H. discus hannai host defense against Vibrio parahemolyticus infection. However studies on its immune system are hindered by the lack of genomic resources. In the present study, we sequenced the transcriptome of control and bacterial challenged H. discus hannai tissues. Totally, 138 MB of reference transcriptome were obtained from de novo assembly of 34 GB clean bases from ten different libraries and annotated with the biological terms (GO and KEGG). A total of 10,575 transcripts exhibiting the differentially expression at least one pair of comparison and the functional annotations highlight genes related to immune response, cell adhesion, immune regulators, redox molecules and mitochondrial coding genes. Mostly, these groups of genes were dominated in hemocytes compared to other tissues. This work is a prerequisite for the identification of those physiological traits controlling H. discus hannai ability to survive against Vibrio infection.
authors.affiliation: Biotechnology Research Division, Aquaculture Industry Department, National Fisheries Research and Development Institute, Busan 619-902, Republic of Korea.
authors.forename: Bo-Hye
authors.initials: BH
authors.lastname: Nam
authors.affiliation: Codes division, Insilicogen Inc., Suwon 441-813, Gyeonggi-do, Republic of Korea.
authors.forename: Myunghee
authors.initials: M
authors.lastname: Jung
authors.affiliation: Codes division, Insilicogen Inc., Suwon 441-813, Gyeonggi-do, Republic of Korea.
authors.forename: Sathiyamoorthy
authors.initials: S
authors.lastname: Subramaniyam
authors.affiliation: Codes division, Insilicogen Inc., Suwon 441-813, Gyeonggi-do, Republic of Korea.
authors.forename: Seung-il
authors.initials: SI
authors.lastname: Yoo
authors.affiliation: Codes division, Insilicogen Inc., Suwon 441-813, Gyeonggi-do, Republic of Korea.
authors.forename: Kesavan
authors.initials: K
authors.lastname: Markkandan
authors.affiliation: Biotechnology Research Division, Aquaculture Industry Department, National Fisheries Research and Development Institute, Busan 619-902, Republic of Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Moon
authors.affiliation: Biotechnology Research Division, Aquaculture Industry Department, National Fisheries Research and Development Institute, Busan 619-902, Republic of Korea.
authors.forename: Young-Ok
authors.initials: YO
authors.lastname: Kim
authors.affiliation: Biotechnology Research Division, Aquaculture Industry Department, National Fisheries Research and Development Institute, Busan 619-902, Republic of Korea.
authors.forename: Dong-Gyun
authors.initials: DG
authors.lastname: Kim
authors.affiliation: Biotechnology Research Division, Aquaculture Industry Department, National Fisheries Research and Development Institute, Busan 619-902, Republic of Korea.
authors.forename: Cheul Min
authors.initials: CM
authors.lastname: An
authors.affiliation: Codes division, Insilicogen Inc., Suwon 441-813, Gyeonggi-do, Republic of Korea.
authors.forename: Younhee
authors.initials: Y
authors.lastname: Shin
authors.affiliation: Codes division, Insilicogen Inc., Suwon 441-813, Gyeonggi-do, Republic of Korea.
authors.forename: Ho-jin
authors.initials: HJ
authors.lastname: Jung
authors.affiliation: Codes division, Insilicogen Inc., Suwon 441-813, Gyeonggi-do, Republic of Korea.
authors.forename: Jun-hyung
authors.initials: JH
authors.lastname: Park
mesh_terms: D000820:Animal Diseases / Q000382:microbiology; D000818:Animals; D049851:Gastropoda / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D054629:Genome, Mitochondrial; D054884:Host-Pathogen Interactions / Q000235:genetics / Q000276:immunology; D007113:Immunity, Innate / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D014735:Vibrio Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary*