title: The antioxidant activities effect of neutral and acidic polysaccharides from Epimedium acuminatum Franch. on Caenorhabditis elegans.
abstract: A neutral polysaccharide (EAP-1N) and an acidic polysaccharide (EAP-2A) were purified from Epimedium acuminatum by DEAE-52 cellulose anion-exchange chromatography and gel-filtration chromatography. Their structures were characterized by chemical composition analysis, high-performance size exclusion chromatography (HPSEC), Fourier transform infrared spectrometry (FT-IR), and gas chromatography-mass spectrometry (GC-MS). Further, their antioxidant activities were investigated both in vitro and in vivo. Results showed that EAP-2A had higher uronic acid content and larger average molecular weight than EAP-1N. Compared with EAP-1N, EAP-2A exhibited significantly scavenging activities against free radical in vitro, as well as strongly stimulating effect on antioxidant enzyme activities (including superoxide dismutases (SOD), catalases (CAT), and glutathione peroxidases (GSH-PX)) and preferably inhibitory effect on lipid peroxidation and protein carboxyl in the mode of Caenorhabditis elegans.
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an 625014, PR China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Xu
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an 625014, PR China.
authors.forename: Shiling
authors.initials: S
authors.lastname: Feng
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an 625014, PR China.
authors.forename: Shian
authors.initials: S
authors.lastname: Shen
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an 625014, PR China.
authors.forename: Handong
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an 625014, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yuan
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an 625014, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an 625014, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an 625014, PR China. Electronic address: dcb@sicau.edu.cn.
authors.forename: Chunbang
authors.initials: C
authors.lastname: Ding
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects*; D002374:Catalase / Q000494:pharmacology; D034181:Epimedium / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D005979:Glutathione Peroxidase / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D010269:Paraquat / Q000494:pharmacology; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D050050:Protein Carbonylation / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000494:pharmacology
chemical_list: D016166:Free Radical Scavengers; D011134:Polysaccharides; D017382:Reactive Oxygen Species; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D010269:Paraquat
keywords: Acidic polysaccharide; Antioxidant activities; Caenorhabditis elegans; Epimedium acuminatum Franch; Neutral polysaccharide