title: Diagnosis of Trypanosoma evansi in bovines by indirect ELISA.
abstract: The diagnosis of Trypanosoma evansi in animals with low parasitaemia is hampered by low diagnostic sensitivity of traditional detection methods. The present study was undertaken with an objective to improve the diagnostic tools for detection of antibodies against T. evansi infection using indirect enzyme-linked immunosorbent assay (ELISA) in bovines. The optimum concentration of antigen, test sera and conjugate were determined as 5 µg per well, 1:10 and 1: 6,000 dilutions, respectively. Among 320 cattle and 382 buffaloes examined in different parts of Rayalaseema region of Andhra Pradesh for T. evansi infection, 36.12 and 31.87 percent were found positive by indirect ELISA, respectively.
authors.affiliation: Department of Veterinary Parasitology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati, 517502 Andhra Pradesh India.
authors.forename: S
authors.initials: S
authors.lastname: Sivajothi
authors.affiliation: Department of Veterinary Parasitology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati, 517502 Andhra Pradesh India.
authors.forename: V C
authors.initials: VC
authors.lastname: Rayulu
authors.affiliation: Department of Veterinary Parasitology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati, 517502 Andhra Pradesh India.
authors.forename: P
authors.initials: P
authors.lastname: Malakondaiah
authors.affiliation: Department of Veterinary Parasitology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati, 517502 Andhra Pradesh India.
authors.forename: D
authors.initials: D
authors.lastname: Sreenivasulu
keywords: Antibodies; Bovines; Diagnosis; Indirect ELISA; Trypanosoma evansi