title: Polymorphic Regions in Fc Gamma Receptor and Tumor Necrosis Factor-α Genes and Susceptibility to Chronic Periodontitis in a Cohort From South India.
abstract: Polymorphisms in the immunoglobulin G Fc receptor II (FcGR) and tumor necrosis factor-α (TNFA) genes are known to influence pathogenesis and severity of several inflammatory conditions. Association of FcGR and TNFA gene polymorphisms with chronic periodontitis (CP) susceptibility has been found to be diverse among different ethnic populations. Objectives of the present study are to determine association of functional single nucleotide polymorphisms (SNPs) in FcGR and TNF-α genes with CP susceptibility in a cohort from South India.
  
  
  
  Polymorphisms of: 1) FCGR2A 131His/Arg (rs1801274); 2) FCGR2B 232Ile/Thr (rs1050501); 3) TNFA -1031T/C (rs1799964); and 4) TNFA -863C/A (rs1800630) were analyzed among patients with healthy gingiva (n = 176) and patients with CP (n = 177). Genotyping was performed using allele-specific real-time polymerase chain reaction assay. Association between CP and SNPs was examined by multivariable logistic regression analysis with adjustment for: 1) age; 2) sex; and 3) oral hygiene index (OHI). Epistatic interaction between FcGR polymorphisms and interleukin 1B (IL1B) +3954C/T (rs1143634) was assessed using multifactorial dimensionality reduction analysis.
  
  
  
  Among four SNPs analyzed, only FCGR2A 131His/Arg showed significant association with CP in a dominant model (odds ratio: 1.6; 95% confidence interval: 1.028 to 2.530). This significance disappeared after correcting for multiple comparisons using Bonferroni analysis, or after adjusting for age, sex, and OHI. A significant redundant interaction between IL1B +3954 C/T and FCGR2A 131His/Arg was observed.
  
  
  
  Study results suggest the variant form of the SNP in FCGR2A 131His/Arg, FCGR2B 232Ile/Thr, TNFA -1031T/C, and TNFA -863C/A are not associated with CP susceptibility in the selected cohort from South India.
authors.affiliation: Department of Periodontology, Sri Ramachandra University, Chennai, India.
authors.forename: Vamsi
authors.initials: V
authors.lastname: Lavu
authors.affiliation: Department of Human Genetics, Sri Ramachandra University.
authors.forename: Vettriselvi
authors.initials: V
authors.lastname: Venkatesan
authors.affiliation: Department of Medical Genetics, Sickle Cell Institute Chhattisgarh, Raipur, India.
authors.forename: Lakkakula V K S
authors.initials: LV
authors.lastname: Bhaskar
authors.affiliation: Department of Periodontology, Sri Ramachandra University, Chennai, India.
authors.forename: Venugopal
authors.initials: V
authors.lastname: Priyanka
authors.affiliation: Department of Bioinformatics, Tamil Nadu Veterinary and Animal Sciences University, Chennai, India.
authors.forename: P
authors.initials: P
authors.lastname: Kumarasamy
authors.affiliation: Department of Human Genetics, Sri Ramachandra University.
authors.forename: Solomon Franklin
authors.initials: SF
authors.lastname: Durairaj Paul
authors.affiliation: Department of Periodontology, Sri Ramachandra University, Chennai, India.
authors.forename: Suresh Ranga
authors.initials: SR
authors.lastname: Rao
mesh_terms: D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000235:genetics*; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007194:India; D020641:Polymorphism, Single Nucleotide; D017452:Receptors, IgG / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: C445323:FCGR2A protein, human; D017452:Receptors, IgG; D014409:Tumor Necrosis Factor-alpha
keywords: Chronic periodontitis; genetic predisposition to diseases; polymorphism, genetic; risk factors; tumor necrosis factor-alpha