title: Construction of recombinant baculoviruses expressing hemagglutinin of H5N1 avian influenza and research on the immunogenicity.
abstract: Recombinant baculoviruses with different promoter and regulatory elements were constructed to enhance the expression of target protein and boost the efficacies of avian influenza vaccine. Hemagglutinin gene was cloned into the baculovirus transfer vectors driven by cytomegaloviru (CMV) and White spot syndrome virus immediate-early promoter one (WSSV ie1) promoter respectively, with different regulatory elements. The recombinant baculoviruses were directly used as vaccines to immunize specific pathogen-free chickens. The protein expression levels of recombinant baculoviruses BV-S-HA and BV-S-ITRs-HA were respectively 2.43 and 2.67 times than that of BV-S-con-HA, while the protein expression levels of BV-A-HA and BV-A-ITRs-HA were respectively 2.44 and 2.69 times than that of BV-S-con-HA. Immunoglobulin G (IgG) antibody levels induced by BV-A and BV-S series recombinant baculovirus were significantly higher than the commercialized vaccine group (P < 0.05). Among the groups with same promoter, the IgG antibody levels induced by the baculovirus containing regulatory elements were significantly higher than control group. Additionally, the immune effects induced by BV-A series recombinant baculoviruses with WSSV ie1 promoter were significantly stronger than the BV-S series recombinant baculoviruses with CMV promoter. The avian influenza vaccine prepared based on baculovirus vector can simultaneously stimulate the humoral and cellular immune responses.
authors.affiliation: Key Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin, China.
authors.forename: Jingping
authors.initials: J
authors.lastname: Ge
authors.affiliation: Key Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: An
authors.affiliation: Key Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin, China.
authors.forename: Dongni
authors.initials: D
authors.lastname: Gao
authors.affiliation: Key Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin, China.
authors.forename: Wenxiang
authors.initials: W
authors.lastname: Ping
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D016367:Baculoviridae / Q000235:genetics; D002642:Chick Embryo; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D007074:Immunoglobulin G / Q000097:blood; D053124:Influenza A Virus, H5N1 Subtype / Q000378:metabolism*; D007252:Influenza Vaccines / Q000276:immunology; D005585:Influenza in Birds / Q000821:virology; D007371:Interferon-gamma / Q000032:analysis; D007376:Interleukin-2 / Q000032:analysis; D015847:Interleukin-4 / Q000032:analysis; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D009976:Orthomyxoviridae Infections / Q000517:prevention & control; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification; D061987:Sf9 Cells / Q000166:cytology / Q000378:metabolism; D046848:White spot syndrome virus 1 / Q000235:genetics
chemical_list: D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007074:Immunoglobulin G; D007252:Influenza Vaccines; D007376:Interleukin-2; D011994:Recombinant Proteins; D015847:Interleukin-4; D007371:Interferon-gamma