title: One-Step Synthesis of Water-Soluble MoS2 Quantum Dots via a Hydrothermal Method as a Fluorescent Probe for Hyaluronidase Detection.
abstract: In this work, a bottom-up strategy is developed to synthesize water-soluble molybdenum disulfide quantum dots (MoS2 QDs) through a simple, one-step hydrothermal method using ammonium tetrathiomolybdate [(NH4)2MoS4] as the precursor and hydrazine hydrate as the reducing agent. The as-synthesized MoS2 QDs are few-layered with a narrow size distribution, and the average diameter is about 2.8 nm. The resultant QDs show excitation-dependent blue fluorescence due to the polydispersity of the QDs. Moreover, the fluorescence can be quenched by hyaluronic acid (HA)-functionalized gold nanoparticles through a photoinduced electron-transfer mechanism. Hyaluronidase (HAase), an endoglucosidase, can cleave HA into proangiogenic fragments and lead to the aggregation of gold nanoparticles. As a result, the electron transfer is blocked and fluorescence is recovered. On the basis of this principle, a novel fluorescence sensor for HAase is developed with a linear range from 1 to 50 U/mL and a detection limit of 0.7 U/mL.
authors.affiliation: Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University , 500 Dongchan Road, Shanghai 200241, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gu
authors.affiliation: Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University , 500 Dongchan Road, Shanghai 200241, China.
authors.forename: Yinghan
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University , 500 Dongchan Road, Shanghai 200241, China.
authors.forename: Cuiling
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University , 500 Dongchan Road, Shanghai 200241, China.
authors.forename: Caiping
authors.initials: C
authors.lastname: Ding
authors.affiliation: Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University , 500 Dongchan Road, Shanghai 200241, China.
authors.forename: Yuezhong
authors.initials: Y
authors.lastname: Xian
mesh_terms: D005456:Fluorescent Dyes; D006046:Gold; D006821:Hyaluronoglucosaminidase; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence; D014867:Water
chemical_list: D005456:Fluorescent Dyes; D014867:Water; D006046:Gold; D006821:Hyaluronoglucosaminidase
keywords: MoS2 quantum dots; biosensor; fluorescence; hyaluronidase; hydrothermal method