title: Host Immunization with Recombinant Proteins to Screen Antigens for Tick Control.
abstract: Ticks (Parasitiformes: Ixodida) are known for their obligate blood feeding habit and their role in transmitting pathogens to various vertebrate hosts. Tick control using chemical acaricides is extensively used particularly in livestock management, but several disadvantages arise from resistance development of many tick species, and concerns on animal product and environmental contamination. Vaccination offers better protection and more cost-effective alternative to application of chemical acaricides, addressing their disadvantages. However, an ideal anti-tick vaccine targeting multiple tick species and all the tick stages is still wanting. Here, we describe the procedures involved in the evaluation of a vaccine candidate antigen against ticks at the laboratory level, from the preparation of recombinant proteins, administration to the rabbit host and monitoring of antibody titer, to tick infestation challenge and determination of the effects of immunization to ticks.
authors.affiliation: Laboratory of Infectious Diseases, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan.
authors.forename: Remil Linggatong
authors.initials: RL
authors.lastname: Galay
authors.affiliation: Laboratory of Food Chemistry, Division of Molecular Functions of Food, Department of Biochemistry and Biotechnology, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Miyata
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, 080-8555, Japan.
authors.forename: Rika
authors.initials: R
authors.lastname: Umemiya-Shirafuji
authors.affiliation: Laboratory of Infectious Diseases, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan.
authors.forename: Masami
authors.initials: M
authors.lastname: Mochizuki
authors.affiliation: National Agricultural and Food Research Organization, 3-1-5 Kannondai, Tsukuba, Ibaraki, 305-0856, Japan.
authors.forename: Kozo
authors.initials: K
authors.lastname: Fujisaki
authors.affiliation: Laboratory of Infectious Diseases, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood / Q000276:immunology; D060829:Arthropod Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D058849:Protein Refolding; D011817:Rabbits; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D013983:Tick Control / Q000379:methods*; D013987:Ticks / Q000235:genetics / Q000276:immunology*; D014611:Vaccination; D014612:Vaccines / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology*
chemical_list: D000906:Antibodies; D060829:Arthropod Proteins; D011994:Recombinant Proteins; D014612:Vaccines
keywords: Anti-arthropod vaccine; Ixodida; Recombinant proteins; Tick control