title: Silver Nanoparticles Induce HePG-2 Cells Apoptosis Through ROS-Mediated Signaling Pathways.
abstract: Recently, silver nanoparticles (AgNPs) have been shown to provide a novel approach to overcome tumors, especially those of hepatocarcinoma. However, the anticancer mechanism of silver nanoparticles is unclear. Thus, the purpose of this study was to estimate the effect of AgNPs on proliferation and activation of ROS-mediated signaling pathway on human hepatocellular carcinoma HePG-2 cells. A simple chemical method for preparing AgNPs with superior anticancer activity has been showed in this study. AgNPs were detected by transmission electronic microscopy (TEM) and energy dispersive X-ray (EDX). The size distribution and zeta potential of silver nanoparticles were detected by Zetasizer Nano. The average size of AgNPs (2 nm) observably increased the cellular uptake by endocytosis. AgNPs markedly inhibited the proliferation of HePG-2 cells through induction of apoptosis with caspase-3 activation and PARP cleavage. AgNPs with dose-dependent manner significantly increased the apoptotic cell population (sub-G1). Furthermore, AgNP-induced apoptosis was found dependent on the overproduction of reactive oxygen species (ROS) and affecting of MAPKs and AKT signaling and DNA damage-mediated p53 phosphorylation to advance HePG-2 cells apoptosis. Therefore, our results show that the mechanism of ROS-mediated signaling pathways may provide useful information in AgNP-induced HePG-2 cell apoptosis.
authors.affiliation: Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou, Guangdong, 510632, People's Republic of China.|Virus Laboratory of Guangzhou Women and Children's Medical Center, Guangzhou, Guangdong, 510120, People's Republic of China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Virus Laboratory of Guangzhou Women and Children's Medical Center, Guangzhou, Guangdong, 510120, People's Republic of China.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Li
authors.affiliation: Virus Laboratory of Guangzhou Women and Children's Medical Center, Guangzhou, Guangdong, 510120, People's Republic of China.
authors.forename: Zhengfang
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Virus Laboratory of Guangzhou Women and Children's Medical Center, Guangzhou, Guangdong, 510120, People's Republic of China.
authors.forename: Mingqi
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Virus Laboratory of Guangzhou Women and Children's Medical Center, Guangzhou, Guangdong, 510120, People's Republic of China.
authors.forename: Tiantian
authors.initials: T
authors.lastname: Xu
authors.affiliation: Virus Laboratory of Guangzhou Women and Children's Medical Center, Guangzhou, Guangdong, 510120, People's Republic of China.
authors.forename: Changbing
authors.initials: C
authors.lastname: Wang
authors.affiliation: Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou, Guangdong, 510632, People's Republic of China. DengNing123@hotmail.com.
authors.forename: Ning
authors.initials: N
authors.lastname: Deng
keywords: Apoptosis; Cellular uptake; Reactive oxygen species; Silver nanoparticles