title: Global gene expression profiling of JMJD6- and JMJD4-depleted mouse NIH3T3 fibroblasts.
abstract: Emerging evidence suggests Jumonji domain-containing proteins are epigenetic regulators in diverse biological processes including cellular differentiation and proliferation. RNA interference-based analyses combined with gene expression profiling can effectively characterize the cellular functions of these enzymes. We found that the depletion of Jumonji domain-containing protein 6 (JMJD6) and its paralog protein Jumonji domain-containing protein 4 (JMJD4) individually by small hairpin RNAs (shRNAs) slowed cell proliferation of mouse NIH3T3 fibroblasts. We subsequently performed gene expression profiling on both JMJD6- and JMJD4-depleted mouse NIH3T3 fibroblasts using the Affymetrix GeneChip Mouse Exon 1.0 ST Array. Here we report the gene profiling datasets along with the experimental procedures. The information can be used to further investigate how JMJD6 and JMJD4 affect gene expression and cellular physiology.
authors.affiliation: Department of Cell and Developmental Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA.
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Hu
authors.affiliation: Department of Cell and Developmental Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA.
authors.forename: Anthony N
authors.initials: AN
authors.lastname: Imbalzano
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000235:genetics; D005347:Fibroblasts / Q000502:physiology*; D020869:Gene Expression Profiling; D020868:Gene Silencing; D056484:Jumonji Domain-Containing Histone Demethylases / Q000235:genetics*; D051379:Mice; D041681:NIH 3T3 Cells; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / Q000235:genetics*; D034741:RNA, Small Interfering; D011956:Receptors, Cell Surface / Q000235:genetics*
chemical_list: C498708:Ptdsr protein, mouse; D034741:RNA, Small Interfering; D011956:Receptors, Cell Surface; D056484:Jumonji Domain-Containing Histone Demethylases; C000610409:JMJD4 protein, mouse; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase