title: Nuclear factor one B (NFIB) encodes a subtype-specific tumour suppressor in glioblastoma.
abstract: Glioblastoma (GBM) is an essentially incurable and rapidly fatal cancer, with few markers predicting a favourable prognosis. Here we report that the transcription factor NFIB is associated with significantly improved survival in GBM. NFIB expression correlates inversely with astrocytoma grade and is lowest in mesenchymal GBM. Ectopic expression of NFIB in low-passage, patient-derived classical and mesenchymal subtype GBM cells inhibits tumourigenesis. Ectopic NFIB expression activated phospho-STAT3 signalling only in classical and mesenchymal GBM cells, suggesting a mechanism through which NFIB may exert its context-dependent tumour suppressor activity. Finally, NFIB expression can be induced in GBM cells by drug treatment with beneficial effects.
authors.affiliation: Brain Cancer Research Unit, QIMR Berghofer Medical Research Institute, Brisbane, 4006, Queensland, Australia.|Leukaemia Foundation Research Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, 4006, Queensland, Australia.
authors.forename: Brett W
authors.initials: BW
authors.lastname: Stringer
authors.affiliation: Queensland Brain Institute, The University of Queensland, Brisbane, 4072, Queensland, Australia.
authors.forename: Jens
authors.initials: J
authors.lastname: Bunt
authors.affiliation: Brain Cancer Research Unit, QIMR Berghofer Medical Research Institute, Brisbane, 4006, Queensland, Australia.|Leukaemia Foundation Research Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, 4006, Queensland, Australia.
authors.forename: Bryan W
authors.initials: BW
authors.lastname: Day
authors.affiliation: Queensland Brain Institute, The University of Queensland, Brisbane, 4072, Queensland, Australia.
authors.forename: Guy
authors.initials: G
authors.lastname: Barry
authors.affiliation: Brain Cancer Research Unit, QIMR Berghofer Medical Research Institute, Brisbane, 4006, Queensland, Australia.|Leukaemia Foundation Research Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, 4006, Queensland, Australia.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Jamieson
authors.affiliation: Brain Cancer Research Unit, QIMR Berghofer Medical Research Institute, Brisbane, 4006, Queensland, Australia.|Leukaemia Foundation Research Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, 4006, Queensland, Australia.
authors.forename: Kathleen S
authors.initials: KS
authors.lastname: Ensbey
authors.affiliation: Brain Cancer Research Unit, QIMR Berghofer Medical Research Institute, Brisbane, 4006, Queensland, Australia.|Leukaemia Foundation Research Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, 4006, Queensland, Australia.
authors.forename: Zara C
authors.initials: ZC
authors.lastname: Bruce
authors.affiliation: Brain Cancer Research Unit, QIMR Berghofer Medical Research Institute, Brisbane, 4006, Queensland, Australia.|Leukaemia Foundation Research Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, 4006, Queensland, Australia.
authors.forename: Kate
authors.initials: K
authors.lastname: Goasdoué
authors.affiliation: Brain Cancer Research Unit, QIMR Berghofer Medical Research Institute, Brisbane, 4006, Queensland, Australia.|Leukaemia Foundation Research Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, 4006, Queensland, Australia.
authors.forename: Hélène
authors.initials: H
authors.lastname: Vidal
authors.affiliation: Leukaemia Foundation Research Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, 4006, Queensland, Australia.
authors.forename: Sara
authors.initials: S
authors.lastname: Charmsaz
authors.affiliation: Leukaemia Foundation Research Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, 4006, Queensland, Australia.
authors.forename: Fiona M
authors.initials: FM
authors.lastname: Smith
authors.affiliation: Leukaemia Foundation Research Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, 4006, Queensland, Australia.
authors.forename: Leanne T
authors.initials: LT
authors.lastname: Cooper
authors.affiliation: Queensland Brain Institute, The University of Queensland, Brisbane, 4072, Queensland, Australia.|School of Biomedical Sciences, The University of Queensland, Brisbane, 4072, Queensland, Australia.
authors.forename: Michael
authors.initials: M
authors.lastname: Piper
authors.affiliation: Brain Cancer Research Unit, QIMR Berghofer Medical Research Institute, Brisbane, 4006, Queensland, Australia.|Leukaemia Foundation Research Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, 4006, Queensland, Australia.|Department of Medicine, The University of Queensland, Brisbane, 4072, Queensland, Australia.
authors.forename: Andrew W
authors.initials: AW
authors.lastname: Boyd
authors.affiliation: Queensland Brain Institute, The University of Queensland, Brisbane, 4072, Queensland, Australia.|School of Biomedical Sciences, The University of Queensland, Brisbane, 4072, Queensland, Australia.
authors.forename: Linda J
authors.initials: LJ
authors.lastname: Richards
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D001932:Brain Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D016147:Genes, Tumor Suppressor / Q000502:physiology; D005909:Glioblastoma / Q000473:pathology*; D064593:Heterografts; D006801:Humans; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D051839:NFI Transcription Factors / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D051839:NFI Transcription Factors; C110754:NFIB protein, human; D025521:Tumor Suppressor Proteins
keywords: GBM subtype; glioblastoma (GBM); glioma; nuclear factor I B (NFIB); tumour suppressor gene