title: MicroRNA-1280 modulates cell growth and invasion of thyroid carcinoma through targeting estrogen receptor α.
abstract: Thyroid cancer (TC) is one of the most common endocrine malignancies, with a steadily increasing incidence and lethality over the last several decades. ERα is a nuclear hormone receptor that has a key role in different cellular process and participates in the development and progression of thyroid cancer. ERα is the predicted target gene of microRNA-1280 (miR-1280). The present study was designed to delineate the role and underlying mechanism of miR-1280 in regulating thyroid cancer through targeting ERα. In our study, we analyzed the expression level of miR-1280 in thyroid cancer and detected significantly lower miR-1280 levels in TC tissue and cell lines compared with adjacent normal tissue or healthy cell line. We then overexpressed miR-1280 by miRNA mimic transfection and inhibited miR-1280 by miRNA inhibitor transfection. The inhibition of miR-1280 significantly elevated proliferation and invasion ability, whereas overexpression of miR-1280 inhibited cell growth and invasion in TC cells. Additionally, the luciferase reporter assay confirmed a targeting reaction between miR-1280 and ERα. Furthermore, overexpression of miR-1280 inhibited ERα and ERK pathway expression in TC cells, indicating that miR-1280 acts as a tumor suppressor by inhibiting the expression of ERα. Taken together, we demonstrated that overexpressed miR-1280 levels in TC cells may promote cell proliferation and invasion by inhibiting ERα, which might provide a new therapeutic target for thyroid cancer.
authors.affiliation: The First Affiliated Hospital of Zhengzhou University Department of Endocrinology Zhengzhou China.
authors.forename: D
authors.initials: D
authors.lastname: Meng
authors.affiliation: The First Affiliated Hospital of Zhengzhou University Department of Orthopaedic Surgery Zhengzhou China.
authors.forename: Z
authors.initials: Z
authors.lastname: Li
authors.affiliation: The First Affiliated Hospital of Zhengzhou University Department of Endocrinology Zhengzhou China.
authors.forename: X
authors.initials: X
authors.lastname: Ma
authors.affiliation: The First Affiliated Hospital of Zhengzhou University Department of Thyroid Surgery Zhengzhou China.
authors.forename: L
authors.initials: L
authors.lastname: Fu
authors.affiliation: The First Affiliated Hospital of Zhengzhou University Department of Endocrinology Zhengzhou China junqinwing@163.com.
authors.forename: G
authors.initials: G
authors.lastname: Qin
mesh_terms: D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D047628:Estrogen Receptor alpha / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics* / Q000473:pathology; D013961:Thyroid Gland / Q000378:metabolism / Q000473:pathology*; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology*
chemical_list: C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; C000603194:MIRN-1280 microRNA, human; D035683:MicroRNAs