title: An Easily Fabricated Electrochemical Sensor Based on a Graphene-Modified Glassy Carbon Electrode for Determination of Octopamine and Tyramine.
abstract: A simple electrochemical sensor has been developed for highly sensitive detection of octopamine and tyramine by electrodepositing reduced graphene oxide (ERGO) nanosheets onto the surface of a glassy carbon electrode (GCE). The electrocatalytic oxidation of octopamine and tyramine is individually investigated at the surface of the ERGO modified glassy carbon electrode (ERGO/GCE) by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Several essential factors including the deposition cycle of reduced graphene oxide nanosheets and the pH of the running buffer were investigated in order to determine the optimum conditions. Furthermore, the sensor was applied to the quantification of octopamine and tyramine by DPV in the concentration ranges from 0.5 to 40 μM and 0.1 to 25 μM, respectively. In addition, the limits of detection of octopamine and tyramine were calculated to be 0.1 μM and 0.03 μM (S/N = 3), respectively. The sensor showed good reproducibility, selectivity and stability. Finally, the sensor successfully detected octopamine and tyramine in commercially available beer with satisfactory recovery ranges which were 98.5%-104.7% and 102.2%-103.1%, respectively. These results indicate the ERGO/GCE based sensor is suitable for the detection of octopamine and tyramine.
authors.affiliation: Research Center for Bioengineering and Sensing Technology, University of Science and Technology, Beijing 100083, China. zhangybslf@126.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Research Center for Bioengineering and Sensing Technology, University of Science and Technology, Beijing 100083, China. zhangmeiqin@ustb.edu.cn.
authors.forename: Meiqin
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Research Center for Bioengineering and Sensing Technology, University of Science and Technology, Beijing 100083, China. wqhwanwan@126.com.
authors.forename: Qianhui
authors.initials: Q
authors.lastname: Wei
authors.affiliation: Research Center for Bioengineering and Sensing Technology, University of Science and Technology, Beijing 100083, China. gaoyongjieustb@126.com.
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Research Center for Bioengineering and Sensing Technology, University of Science and Technology, Beijing 100083, China. 13120328993@163.com.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Guo
authors.affiliation: Department of Zoology, College of Science, P. O. Box 2455, King Saud University, Riyadh 11451, Saudi Arabia. Kghanim@ksu.edu.sa.
authors.forename: Khalid A
authors.initials: KA
authors.lastname: Al-Ghanim
authors.affiliation: Department of Zoology, College of Science, P. O. Box 2455, King Saud University, Riyadh 11451, Saudi Arabia. shahidmahboob60@hotmail.com.|Department of Zoology, Government College University, Fsisalabad 38000, Pakistan. shahidmahboob60@hotmail.com.
authors.forename: Shahid
authors.initials: S
authors.lastname: Mahboob
authors.affiliation: Research Center for Bioengineering and Sensing Technology, University of Science and Technology, Beijing 100083, China. zhangxueji@ustb.edu.cn.
authors.forename: Xueji
authors.initials: X
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D002273:Carcinogens / Q000302:isolation & purification* / Q000633:toxicity; D055664:Electrochemical Techniques; D004566:Electrodes; D005504:Food Analysis; D005898:Glass / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006801:Humans; D009655:Octopamine / Q000302:isolation & purification* / Q000633:toxicity; D010084:Oxidation-Reduction; D014439:Tyramine / Q000302:isolation & purification* / Q000633:toxicity
chemical_list: D002273:Carcinogens; D009655:Octopamine; D002244:Carbon; D006108:Graphite; D014439:Tyramine
keywords: electrochemical sensor; graphene; octopamine; tyramine