title: Chitin enhances biocontrol of Rhodotorula mucilaginosa to postharvest decay of peaches.
abstract: Biological control using microbial antagonists is a promising alternative approach to synthetic fungicides. However, effective biological control requires enhancing the consistency and efficacy of the antagonists used to control postharvest diseases. This study investigated the effect of chitin on the biocontrol efficacy of Rhodotorula mucilaginosa against blue mold and Rhizopus decay of peaches and on the protein expression profiles of R. mucilaginosa. The antagonistic activity of R. mucilaginosa harvested from the nutrient yeast dextrose broth (NYDB) with 0.5% chitin added was significantly improved compared with culture in NYDB without chitin. The R. mucilaginosa population cultured in chitin-supplement NYDB and nutrient yeast chitin borth (NYCB) harvested from peach wounds was more than that of R. mucilaginosa cultured in NYDB without chitin throughout the storage period except at 1 d. The protein expression profiles findings revealed that there were several differentially expressed proteins of R. mucilaginosa in the 0.5% chitin-supplemented NYDB and NYCB compared with that of R. mucilaginosa in NYDB. Most of these were cellular proteomes relating to the primary metabolic reactions such as glycoside hydrolases, phosphoribosyl pyrophosphate, and NADH dehydrogenases. Some proteins were also related to signal transmission and stress response.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, People's Republic of China. Electronic address: zhanghongyin126@126.com.
authors.forename: Hongyin
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, People's Republic of China.
authors.forename: Qiya
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, Jiangsu, People's Republic of China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Ge
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, Jiangsu, People's Republic of China.
authors.forename: Guochao
authors.initials: G
authors.lastname: Zhang
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, People's Republic of China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, People's Republic of China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Zhang
mesh_terms: D061046:Biological Control Agents / Q000737:chemistry* / Q000494:pharmacology; D002686:Chitin / Q000737:chemistry / Q000494:pharmacology*; D005658:Fungi / Q000187:drug effects / Q000472:pathogenicity; D000068239:Prunus persica / Q000254:growth & development / Q000469:parasitology*; D012233:Rhizopus / Q000187:drug effects / Q000472:pathogenicity; D012248:Rhodotorula / Q000737:chemistry*
chemical_list: D061046:Biological Control Agents; D002686:Chitin
keywords: Antagonistic activity; Chitin; Rhodotorula mucilaginosa