title: Human colon cancer HT-29 cell death responses to doxorubicin and Morus Alba leaves flavonoid extract.
abstract: The mechanistic basis for the biological properties of Morus alba flavonoid extract (MFE) and chemotherapy drug of doxorubicin on human colon cancer HT-29 cell line death are unknown. The effect of doxorubicin and flavonoid extract on colon cancer HT-29 cell line death and identification of APC gene expression and PARP concentration of HT-29 cell line were investigated. The results showed that flavonoid extract and doxorubicin induce a dose dependent cell death in HT-29 cell line. MFE and doxorubicin exert a cytotoxic effect on human colon cancer HT-29 cell line by probably promoting or induction of apoptosis.
authors.affiliation: Iran University of Medical Sciences Department of Biochemistry, Faculty of Medicine Tehran Iran fallah.s@iums.ac.ir.
authors.forename: S
authors.initials: S
authors.lastname: Fallah
authors.affiliation: Tehran University of Medical Sciences Department of Biochemistry Tehran Iran.
authors.forename: A
authors.initials: A
authors.lastname: Karimi
authors.affiliation: Tehran University of Medical Sciences Department of Biochemistry Tehran Iran.
authors.forename: G
authors.initials: G
authors.lastname: Panahi
authors.affiliation: Tehran University of Medical Sciences Department of Biochemistry Tehran Iran.
authors.forename: S
authors.initials: S
authors.lastname: Gerayesh Nejad
authors.affiliation: Iran University of Medical Sciences Department of Biochemistry, Faculty of Medicine Tehran Iran.
authors.forename: R
authors.initials: R
authors.lastname: Fadaei
authors.affiliation: University of Alberta Department of Medical Genetics, Faculty of Medicine and Dentistry Edmonton Canada.
authors.forename: M
authors.initials: M
authors.lastname: Seifi
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D017491:Genes, APC / Q000187:drug effects; D019073:HT29 Cells; D006801:Humans; D031625:Morus / Q000737:chemistry*; D010936:Plant Extracts; D018515:Plant Leaves / Q000737:chemistry; D011065:Poly(ADP-ribose) Polymerases / Q000032:analysis
chemical_list: D000903:Antibiotics, Antineoplastic; D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids; D010936:Plant Extracts; D004317:Doxorubicin; D011065:Poly(ADP-ribose) Polymerases