title: Detection of colorectal dysplasia using fluorescently labelled lectins.
abstract: Colorectal cancer screening using conventional colonoscopy lacks molecular information and can miss dysplastic lesions. We tested here the ability of fluorescently labelled lectins to distinguish dysplasia from normal tissue when sprayed on to the luminal surface epithelium of freshly resected colon tissue from the Apc(min) mouse and when applied to fixed human colorectal tissue sections. Wheat germ agglutinin (WGA) showed significantly decreased binding to adenomas in the mouse tissue and in sections of human colon from 47 patients. Changes in WGA binding to the human surface epithelium allowed regions containing normal epithelium (NE) or hyperplastic polyps (HP) to be distinguished from regions containing low-grade dysplasia (LGD), high-grade dysplasia (HGD) or carcinoma (C), with 81% sensitivity, 87% specificity and 93% positive predictive value (PPV). Helix pomatia agglutinin (HGA) distinguished epithelial regions containing NE from regions containing HP, LGD, HGD or C, with 89% sensitivity, 87% specificity and 97% PPV. The decreased binding of WGA and HPA to the luminal surface epithelium in human dysplasia suggests that these lectins may enable more sensitive detection of disease in the clinic using fluorescence colonoscopy.
authors.affiliation: Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Cambridge, UK.|Department of Biochemistry, University of Cambridge, Cambridge, UK.
authors.forename: Joe Chin-Hun
authors.initials: JC
authors.lastname: Kuo
authors.affiliation: Department of Pathology, Division of Molecular Histopathology, University of Cambridge, Addenbrooke's Hospital, Cambridge, UK.|MRC, Laboratory of Molecular Biology, Hills Road, Cambridge, UK.
authors.forename: Ashraf E K
authors.initials: AE
authors.lastname: Ibrahim
authors.affiliation: Cambridge Clinical Trials Unit, University of Cambridge, Cambridge, UK.
authors.forename: Sarah
authors.initials: S
authors.lastname: Dawson
authors.affiliation: Statistics and Epidemiology Unit &Cancer Research Centre, Division of Health Sciences, Warwick Medical School, The University of Warwick, Coventry, UK.
authors.forename: Deepak
authors.initials: D
authors.lastname: Parashar
authors.affiliation: Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Cambridge, UK.
authors.forename: William J
authors.initials: WJ
authors.lastname: Howat
authors.affiliation: Department of Pathology, Division of Molecular Histopathology, University of Cambridge, Addenbrooke's Hospital, Cambridge, UK.
authors.forename: Kiran
authors.initials: K
authors.lastname: Guttula
authors.affiliation: Cambridge Colorectal Unit, Addenbrooke's Hospital, Cambridge, UK.
authors.forename: Richard
authors.initials: R
authors.lastname: Miller
authors.affiliation: Cambridge Colorectal Unit, Addenbrooke's Hospital, Cambridge, UK.
authors.forename: Nicola S
authors.initials: NS
authors.lastname: Fearnhead
authors.affiliation: Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Cambridge, UK.
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: Winton
authors.affiliation: Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Cambridge, UK.
authors.forename: André A
authors.initials: AA
authors.lastname: Neves
authors.affiliation: Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Cambridge, UK.|Department of Biochemistry, University of Cambridge, Cambridge, UK.
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Brindle
mesh_terms: D000236:Adenoma / Q000378:metabolism* / Q000473:pathology; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D003106:Colon / Q000378:metabolism / Q000473:pathology; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D037102:Lectins / Q000378:metabolism*; D008297:Male; D051379:Mice; D008875:Middle Aged; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D005456:Fluorescent Dyes; D037102:Lectins