title: The use of immunohistochemistry in detection of perineural invasion in mucoepidermoid carcinoma.
abstract: OBJECTIVE
  To retrospectively study the prevalence of perineural invasion (PNI) in cases of mucoepidermoid carcinoma (MEC). The study evaluated if previously assessed PNI would be increased by re-review of the original hematoxylin and eosin-stained (H&E) slides and also review of slides reacted immunohistochemically with S100 to enhance nerve visualization and whether this is associated with clinical outcome.
  
  
  METHODS
  Thirty-one cases were reviewed for PNI with H&E-stained slides as well as S-100-reacted slides. These results were compared with the original pathology report's PNI status when available (13 of 31). Subject demographic characteristics and clinical outcome were collected from electronic medical records.
  
  
  RESULTS
  PNI was identified in 23% (3 of 13) of tumors in the original reports, 13% (4 of 31) of the authors' re-review of the slides, and 29% (9 of 31) by immunohistochemical assessment for S100. PNI and larger-diameter nerve involvement were significantly associated with death at 5-year follow-up.
  
  
  CONCLUSIONS
  Immunohistochemical assessment for S100 improves the accuracy of PNI determination. PNI is a significant factor in the survival outcome of cases of MEC.
authors.affiliation: College of Dentistry, University of Iowa, Iowa City, Iowa, USA. Electronic address: Emily-Lanzel@uiowa.edu.
authors.forename: Emily
authors.initials: E
authors.lastname: Lanzel
authors.affiliation: Department of Pathology, The Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Robinson
authors.affiliation: Department of Biostatistics, College of Public Health, University of Iowa, Iowa City, Iowa, USA.
authors.forename: Miriam Bridget
authors.initials: MB
authors.lastname: Zimmerman
authors.affiliation: College of Dental Medicine, Roseman University of Health Sciences, South Jordan, Utah, USA.
authors.forename: Ali
authors.initials: A
authors.lastname: Pourian
authors.affiliation: College of Dentistry, University of Iowa, Iowa City, Iowa, USA.
authors.forename: John W
authors.initials: JW
authors.lastname: Hellstein
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018277:Carcinoma, Mucoepidermoid / Q000473:pathology* / Q000628:therapy; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness / Q000473:pathology*; D009367:Neoplasm Staging; D012189:Retrospective Studies; D012468:Salivary Gland Neoplasms / Q000473:pathology* / Q000628:therapy