title: Hydroxyapatite-hybridized chitosan/chitin whisker bionanocomposite fibers for bone tissue engineering applications.
abstract: Biomimetic nanofibrous scaffolds derived from natural biopolymers for bone tissue engineering applications require good mechanical and biological performances including biomineralization. The present work proposes the utility of chitin whisker (CTWK) to enhance mechanical properties of chitosan/poly(vinyl alcohol) (CS/PVA) nanofibers and to offer osteoblast cell growth with hydroxyapatite (HA) mineralization. By using diacid as a solvent, electrospun CS/PVA nanofibrous membranes containing CTWK can be easily obtained. The dimension stability of nanofibrous CS/PVA/CTWK bionanocomposite is further controlled by exposing to glutaraldehyde vapor. The nanofibrous membranes obtained allow mineralization of HA in concentrated simulated body fluid resulting in an improvement of Young's modulus and tensile strength. The CTWK combined with HA in bionanocomposite is a key to promote osteoblast cell adhesion and proliferation. The present work, for the first time, demonstrates the use of CTWKs for bionanocomposite fibers of chitosan and its hydroxyapatite biomineralization with the function in osteoblast cell culture. These hydroxyapatite-hybridized CS/PVA/CTWK bionanocomposite fibers (CS/PVA/CTWK-HA) offer a great potential for bone tissue engineering applications.
authors.affiliation: National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency, Thailand Science Park, Pathumthani 12120, Thailand. Electronic address: autchara@nanotec.or.th.
authors.forename: Autchara
authors.initials: A
authors.lastname: Pangon
authors.affiliation: National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency, Thailand Science Park, Pathumthani 12120, Thailand.
authors.forename: Somsak
authors.initials: S
authors.lastname: Saesoo
authors.affiliation: National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency, Thailand Science Park, Pathumthani 12120, Thailand.
authors.forename: Nattika
authors.initials: N
authors.lastname: Saengkrit
authors.affiliation: National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency, Thailand Science Park, Pathumthani 12120, Thailand.
authors.forename: Uracha
authors.initials: U
authors.lastname: Ruktanonchai
authors.affiliation: National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency, Thailand Science Park, Pathumthani 12120, Thailand.
authors.forename: Varol
authors.initials: V
authors.lastname: Intasanta
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D001842:Bone and Bones / Q000166:cytology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002686:Chitin / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D055595:Mechanical Phenomena; D051379:Mice; D053761:Nanocomposites / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D011142:Polyvinyl Alcohol / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D002686:Chitin; D011142:Polyvinyl Alcohol; D048271:Chitosan; D017886:Durapatite
keywords: Bionanocomposite; Bone tissue engineering scaffold; Chitin whisker; Chitosan; Electrospun nanofiber; Hydroxyapatite