title: Pathogenicity of Genetically Similar, H5N1 Highly Pathogenic Avian Influenza Virus Strains in Chicken and the Differences in Sensitivity among Different Chicken Breeds.
abstract: Differences in the pathogenicity of genetically closely related H5N1 highly pathogenic avian influenza viruses (HPAIVs) were evaluated in White Leghorn chickens. These viruses varied in the clinical symptoms they induced, including lethality, virus shedding, and replication in host tissues. A comparison of the host responses in the lung, brain, and spleen suggested that the differences in viral replication efficiency were related to the host cytokine response at the early phase of infection, especially variations in the proinflammatory cytokine IL-6. Based on these findings, we inoculated the virus that showed the mildest pathogenicity among the five tested, A/pigeon/Thailand/VSMU-7-NPT/2004, into four breeds of Thai indigenous chicken, Phadu-Hung-Dang (PHD), Chee, Dang, and Luang-Hung-Khao (LHK), to explore effects of genetic background on host response. Among these breeds, Chee, Dang, and LHK showed significantly longer survival times than White Leghorns. Virus shedding from dead Thai indigenous chickens was significantly lower than that from White Leghorns. Although polymorphisms were observed in the Mx and MHC class I genes, there was no significant association between the polymorphisms in these loci and resistance to HPAIV.
authors.affiliation: Thailand-Japan Zoonotic Diseases Collaborating Center (ZDCC), Kasetlang, Chatuchak, Bangkok, Thailand.|Research Team for Zoonotic Diseases, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Kannondai, Tsukuba, Ibaraki, Japan.|Transboundary Animal Diseases Research Center, Joint Faculty of Veterinary, Kagoshima University, Korimoto, Kagoshima, Japan.
authors.forename: Aya
authors.initials: A
authors.lastname: Matsuu
authors.affiliation: Thailand-Japan Zoonotic Diseases Collaborating Center (ZDCC), Kasetlang, Chatuchak, Bangkok, Thailand.|Research Team for Zoonotic Diseases, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Kannondai, Tsukuba, Ibaraki, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kobayashi
authors.affiliation: National Institute of Animal Health, Kasetklang, Chatuchak, Bangkok, Thailand.
authors.forename: Tuangthong
authors.initials: T
authors.lastname: Patchimasiri
authors.affiliation: Department of Molecular Life Science, Tokai University School of Medicine, Shimokasuya Isehara, Kanagawa, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Shiina
authors.affiliation: Department of Molecular Life Science, Tokai University School of Medicine, Shimokasuya Isehara, Kanagawa, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Suzuki
authors.affiliation: The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals, Faculty of Veterinary Science, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom, Thailand.
authors.forename: Kridsada
authors.initials: K
authors.lastname: Chaichoune
authors.affiliation: The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals, Faculty of Veterinary Science, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom, Thailand.
authors.forename: Parntep
authors.initials: P
authors.lastname: Ratanakorn
authors.affiliation: Thailand-Japan Zoonotic Diseases Collaborating Center (ZDCC), Kasetlang, Chatuchak, Bangkok, Thailand.|Research Team for Zoonotic Diseases, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Kannondai, Tsukuba, Ibaraki, Japan.
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Hiromoto
authors.affiliation: Thailand-Japan Zoonotic Diseases Collaborating Center (ZDCC), Kasetlang, Chatuchak, Bangkok, Thailand.|Research Team for Zoonotic Diseases, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Kannondai, Tsukuba, Ibaraki, Japan.|Influenza and Prion Disease Research Center, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Kannnondai, Tsukuba, Ibaraki, Japan.
authors.forename: Haruka
authors.initials: H
authors.lastname: Abe
authors.affiliation: National Institute of Animal Health, Kasetklang, Chatuchak, Bangkok, Thailand.
authors.forename: Sujira
authors.initials: S
authors.lastname: Parchariyanon
authors.affiliation: Thailand-Japan Zoonotic Diseases Collaborating Center (ZDCC), Kasetlang, Chatuchak, Bangkok, Thailand.|Research Team for Zoonotic Diseases, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Kannondai, Tsukuba, Ibaraki, Japan.|Influenza and Prion Disease Research Center, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Kannnondai, Tsukuba, Ibaraki, Japan.
authors.forename: Takehiko
authors.initials: T
authors.lastname: Saito
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics; D001947:Breeding; D002645:Chickens / Q000145:classification / Q000235:genetics / Q000821:virology*; D016207:Cytokines / Q000235:genetics; D058507:Host Specificity; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000145:classification / Q000472:pathogenicity*; D005585:Influenza in Birds / Q000235:genetics / Q000821:virology*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014774:Virulence; D014779:Virus Replication; D017201:Virus Shedding
chemical_list: D030161:Avian Proteins; D016207:Cytokines