title: RETRACTED: 6-Pyrazolylpurine as an Artificial Nucleobase for Metal-Mediated Base Pairing in DNA Duplexes.
abstract: The artificial nucleobase 6-pyrazol-1-yl-purine (6PP) has been investigated with respect to its usability in metal-mediated base pairing. As was shown by temperature-dependent UV spectroscopy, 6PP may form weakly stabilizing 6PP-Ag(I)-6PP homo base pairs. Interestingly, 6PP can be used to selectively recognize a complementary pyrimidine nucleobase. The addition of Ag(I) to a DNA duplex comprising a central 6PP:C mispair (C = cytosine) leads to a slight destabilization of the duplex. In contrast, a stabilizing 6PP-Ag(I)-T base pair is formed with a complementary thymine (T) residue. It is interesting to note that 6PP is capable of differentiating between the pyrimidine moieties despite the fact that it is not as sterically crowded as 6-(3,5-dimethylpyrazol-1-yl)purine, an artificial nucleobase that had previously been suggested for the recognition of nucleic acid sequences via the formation of a metal-mediated base pair. Hence, the additional methyl groups of 6-(3,5-dimethylpyrazol-1-yl)purine may not be required for the specific recognition of the complementary nucleobase.
authors.affiliation: Institut für Anorganische und Analytische Chemie, Westfälische Wilhelms-Universität Münster, Corrensstraße 30, 48149 Münster, Germany. j_leon02@uni-muenster.de.
authors.forename: J Christian
authors.initials: JC
authors.lastname: Léon
authors.affiliation: Institut für Anorganische und Analytische Chemie, Westfälische Wilhelms-Universität Münster, Corrensstraße 30, 48149 Münster, Germany. indranilsinha14@gmail.com.|NRW Graduate School of Chemistry, Westfälische Wilhelms-Universität Münster, Corrensstraße 30, 48149 Münster, Germany. indranilsinha14@gmail.com.
authors.forename: Indranil
authors.initials: I
authors.lastname: Sinha
authors.affiliation: Institut für Anorganische und Analytische Chemie, Westfälische Wilhelms-Universität Münster, Corrensstraße 30, 48149 Münster, Germany. mueller.j@uni-muenster.de.|NRW Graduate School of Chemistry, Westfälische Wilhelms-Universität Münster, Corrensstraße 30, 48149 Münster, Germany. mueller.j@uni-muenster.de.
authors.forename: Jens
authors.initials: J
authors.lastname: Müller
mesh_terms: D020029:Base Pairing; D004247:DNA / Q000737:chemistry*; D011687:Purines / Q000737:chemistry*; D011720:Pyrazoles / Q000737:chemistry*; D012834:Silver / Q000737:chemistry
chemical_list: C000598023:6-(3,5-dimethylpyrazol-1-yl)purine; D011687:Purines; D011720:Pyrazoles; D012834:Silver; D004247:DNA
keywords: cytosine; metal-mediated base pair; purine; thymine