title: Der f 21, a novel allergen from dermatophagoides farina.
abstract: The Dermatophagoides farina (D. farina) allergens are an important factor contributing to allergic disease. To identify new allergens is important for diagnosis and treatment of allergic diseases. In this study, we sought to characterize the biological activity of Der f 21 of D. farina. The recombinant Der f 21 protein was characterized by western-blot, ELISA and Skin prick test using clinic patient's serum.An allergic asthma mouse model was established with the rDer f 21 as a specific antigen. The results showed that the sera from 28.9% in 38 dust mite allergic children displayed positive results in response to rDer f 21, and 42% in 98 dust mite allergic patients displayed positive response in skin prick test. In addition, Immune inhibition assays showed there was IgE cross-reactivity between rDer f 21 and rDer f 5. Moreover, an allergic asthma mouse model was established. Airway hyperresponsiveness, serum specific IgE, IgG1, eosinophil infiltration in the allergic mice, interleukin-4(IL-4) and interferon-γ (INF-γ) from spleen cells were markedly increased in the allergic mice. The results demonstrate that Der f 21 is a novel allergen.
authors.affiliation: Otolaryngology Institutes, Allergy and Immunology Research Center, Shenzhen UniversityShenzhen 518060, China; The State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen University School of Medicine3688 Nanhai Blvd., Shenzhen 518060, China.
authors.forename: Yulan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Otolaryngology Institutes, Allergy and Immunology Research Center, Shenzhen UniversityShenzhen 518060, China; The State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen University School of Medicine3688 Nanhai Blvd., Shenzhen 518060, China.
authors.forename: Congli
authors.initials: C
authors.lastname: Jiang
authors.affiliation: The State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen University School of Medicine 3688 Nanhai Blvd., Shenzhen 518060, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.affiliation: The State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen University School of Medicine 3688 Nanhai Blvd., Shenzhen 518060, China.
authors.forename: Haiqiong
authors.initials: H
authors.lastname: Yu
authors.affiliation: The State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen University School of Medicine 3688 Nanhai Blvd., Shenzhen 518060, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Shenzhen Kay Laboratory of Shenzhen, Shenzhen Longgang Central Hospital, ENT Institute Shenzhen, China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Fan
authors.affiliation: The State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen University School of Medicine 3688 Nanhai Blvd., Shenzhen 518060, China.
authors.forename: Jianli
authors.initials: J
authors.lastname: Lin
authors.affiliation: Otolaryngology Institutes, Allergy and Immunology Research Center, Shenzhen UniversityShenzhen 518060, China; The State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen University School of Medicine3688 Nanhai Blvd., Shenzhen 518060, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Liu
authors.affiliation: The State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen University School of Medicine 3688 Nanhai Blvd., Shenzhen 518060, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Otolaryngology Institutes, Allergy and Immunology Research Center, Shenzhen UniversityShenzhen 518060, China; The State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen University School of Medicine3688 Nanhai Blvd., Shenzhen 518060, China.
authors.forename: Pingchang
authors.initials: P
authors.lastname: Yang
authors.affiliation: Otolaryngology Institutes, Allergy and Immunology Research Center, Shenzhen UniversityShenzhen 518060, China; The State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen University School of Medicine3688 Nanhai Blvd., Shenzhen 518060, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Liu
keywords: Allergen; Der f 21; airway; biological activity; dust mite