title: A reliable Raman-spectroscopy-based approach for diagnosis, classification and follow-up of B-cell acute lymphoblastic leukemia.
abstract: Acute lymphoblastic leukemia type B (B-ALL) is a neoplastic disorder that shows high mortality rates due to immature lymphocyte B-cell proliferation. B-ALL diagnosis requires identification and classification of the leukemia cells. Here, we demonstrate the use of Raman spectroscopy to discriminate normal lymphocytic B-cells from three different B-leukemia transformed cell lines (i.e., RS4;11, REH, MN60 cells) based on their biochemical features. In combination with immunofluorescence and Western blotting, we show that these Raman markers reflect the relative changes in the potential biological markers from cell surface antigens, cytoplasmic proteins, and DNA content and correlate with the lymphoblastic B-cell maturation/differentiation stages. Our study demonstrates the potential of this technique for classification of B-leukemia cells into the different differentiation/maturation stages, as well as for the identification of key biochemical changes under chemotherapeutic treatments. Finally, preliminary results from clinical samples indicate high consistency of, and potential applications for, this Raman spectroscopy approach.
authors.affiliation: Institute of Protein Biochemistry, National Research Council, Via P. Castellino 111, 80131 Naples, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Managò
authors.affiliation: Institute of Protein Biochemistry, National Research Council, Via P. Castellino 111, 80131 Naples, Italy.
authors.forename: Carmen
authors.initials: C
authors.lastname: Valente
authors.affiliation: IRCCS SDN Istituto di Ricerca Diagnostica e Nucleare, Via E. Gianturco 113, 80143 Naples, Italy.
authors.forename: Peppino
authors.initials: P
authors.lastname: Mirabelli
authors.affiliation: Institute of Protein Biochemistry, National Research Council, Via P. Castellino 111, 80131 Naples, Italy.
authors.forename: Diego
authors.initials: D
authors.lastname: Circolo
authors.affiliation: UOSC Servizio di Immunoematologia e Medicina Trasfusionale, AORN Cardarelli Hospital, Via A. Cardarelli 9, 80131, Naples, Italy.
authors.forename: Filomena
authors.initials: F
authors.lastname: Basile
authors.affiliation: Institute of Protein Biochemistry, National Research Council, Via P. Castellino 111, 80131 Naples, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Corda
authors.affiliation: Institute of Protein Biochemistry, National Research Council, Via P. Castellino 111, 80131 Naples, Italy.
authors.forename: Anna Chiara
authors.initials: AC
authors.lastname: De Luca
mesh_terms: D000970:Antineoplastic Agents / Q000627:therapeutic use; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D006801:Humans; D016130:Immunophenotyping; D015448:Leukemia, B-Cell / Q000175:diagnosis* / Q000188:drug therapy / Q000276:immunology / Q000473:pathology; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D000970:Antineoplastic Agents