title: Protein profile changes in the frontotemporal lobes in human severe traumatic brain injury.
abstract: Severe traumatic brain injury (sTBI) is a serious public health issue with high morbidity and mortality rates. Previous proteomic studies on sTBI have mainly focused on human cerebrospinal fluid and serum, as well as on brain protein changes in murine models. However, human proteomic data in sTBI brain is still scarce. We used proteomic and bioinformatic strategies to investigate variations in protein expression levels in human brains after sTBI, using samples from the Department of Neurosurgery, Affiliated Hospital of Hebei University (Hebei, China). Our proteomic data identified 4031 proteins, of which 160 proteins were overexpressed and 5 proteins were downregulated. Bioinformatics analysis showed significant changes in biological pathways including glial cell differentiation, complement activation and apolipoprotein catalysis in the statin pathway. Western blot verification of protein changes in a subset of the available tissue samples showed results that were consistent with the proteomic data. This study is one of the first to investigate the whole proteome of human sTBI brains, and provide a characteristic signature and overall landscape of the sTBI brain proteome.
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Hebei University, Baoding 071000, China; National Key Laboratory of Medical Molecular Biology and Department of Immunology; Institute of Basic Medical Sciences; Chinese Academy of Medical Sciences, Beijing 100005, China.
authors.forename: Benhong
authors.initials: B
authors.lastname: Xu
authors.affiliation: Department of Neurosurgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Tian
authors.affiliation: National Key Laboratory of Medical Molecular Biology and Department of Immunology; Institute of Basic Medical Sciences; Chinese Academy of Medical Sciences, Beijing 100005, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.affiliation: National Key Laboratory of Medical Molecular Biology and Department of Immunology; Institute of Basic Medical Sciences; Chinese Academy of Medical Sciences, Beijing 100005, China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Zhan
authors.affiliation: Department of Neurosurgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Renzhi
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Hebei University, Baoding 071000, China; Department of Neurosurgery, Tsinghua Changgung Hospital, Beijing 102218, China. Electronic address: ghuoyi@gmail.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Hebei University, Baoding 071000, China; National Key Laboratory of Medical Molecular Biology and Department of Immunology; Institute of Basic Medical Sciences; Chinese Academy of Medical Sciences, Beijing 100005, China. Electronic address: wei.ge@chem.ox.ac.uk.
authors.forename: Wei
authors.initials: W
authors.lastname: Ge
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000070642:Brain Injuries, Traumatic / Q000235:genetics* / Q000378:metabolism*; D019295:Computational Biology; D005260:Female; D005625:Frontal Lobe / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D040901:Proteomics; D013702:Temporal Lobe / Q000378:metabolism*; D055815:Young Adult
keywords: Human; Proteomics; Severe traumatic brain injury; TMT (tandem mass tags)