title: PRELIMINARY REPORT ON THE PUTATIVE ASSOCIATION OF IL10 -3575 T/A GENETIC POLYMORPHISM WITH MALARIA SYMPTOMS.
abstract: Only a small percentage of individuals living in endemic areas develop severe malaria suggesting that host genetic factors may play a key role. This study has determined the frequency of single nucleotide polymorphisms (SNPs) in some pro and anti-inflammatory cytokine gene sequences: IL6 (-174; rs1800795), IL12p40 (+1188; rs3212227), IL4 (+33; rs2070874), IL10 (-3575; rs1800890) and TGFb1 (+869; rs1800470), by means of PCR-RFLP. Blood samples were collected from 104 symptomatic and 37 asymptomatic subjects. Laboratory diagnosis was assessed by the thick blood smear test and nested-PCR. No association was found between IL6 (-174), IL12p40 (+1188), IL4 (+33), IL10 (- 3575), TGFb1 (+869) SNPs and malaria symptoms. However, regarding the IL10 -3575 T/A SNP, there were significantly more AA and AT subjects, carrying the polymorphic allele A, in the symptomatic group (c2 = 4.54, p = 0.01, OR = 0.40 [95% CI - 0.17- 0.94]). When the analysis was performed by allele, the frequency of the polymorphic allele A was also significantly higher in the symptomatic group (c2 = 4.50, p = 0.01, OR = 0.45 [95% CI - 0.21-0.95]). In conclusion, this study has suggested the possibility that the IL10 - 3575 T/A SNP might be associated with the presence and maintenance of malaria symptoms in individuals living in endemic areas. Taking into account that this polymorphism is related to decreased IL10 production, a possible role of this SNP in the pathophysiology of malaria is also suggested, but replication studies with a higher number of patients and evaluation of IL10 levels are needed for confirmation.
authors.affiliation: Laboratory of Seroepidemiology and Immunobiology, Institute of Tropical Medicine, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Wilson
authors.initials: W
authors.lastname: Domingues
authors.affiliation: Laboratory of Seroepidemiology and Immunobiology, Institute of Tropical Medicine, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Kelly Aparecida
authors.initials: KA
authors.lastname: Kanunfre
authors.affiliation: Laboratory of Seroepidemiology and Immunobiology, Institute of Tropical Medicine, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Jonatas Cristian
authors.initials: JC
authors.lastname: Rodrigues
authors.affiliation: Laboratory of Seroepidemiology and Immunobiology, Institute of Tropical Medicine, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Leandro Emidio
authors.initials: LE
authors.lastname: Teixeira
authors.affiliation: Laboratory of Seroepidemiology and Immunobiology, Institute of Tropical Medicine, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Lidia
authors.initials: L
authors.lastname: Yamamoto
authors.affiliation: Laboratory of Seroepidemiology and Immunobiology, Institute of Tropical Medicine, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Thelma Suely
authors.initials: TS
authors.lastname: Okay
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008288:Malaria / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D055815:Young Adult
chemical_list: D016753:Interleukin-10