title: Skeletal Muscle-Derived Stem/Progenitor Cells: A Potential Strategy for the Treatment of Acute Kidney Injury.
abstract: Skeletal muscle-derived stem/progenitor cells (MDSPCs) have been thoroughly investigated and already used in preclinical studies. However, therapeutic potential of MDSPCs isolated using preplate isolation technique for acute kidney injury (AKI) has not been evaluated. We aimed to characterize rat MDSPCs, compare them with bone marrow mesenchymal stem cells (BM-MSCs), and evaluate the feasibility of MDSPCs therapy for gentamicin-induced AKI in rats. We have isolated and characterized rat MDSPCs and BM-MSCs. Characteristics of rat BM-MSCs and MDSPCs were assessed by population doubling time, flow cytometry, immunofluorescence staining, RT-PCR, and multipotent differentiation capacity. Gentamicin-induced AKI model in rat was used to examine MDSPCs therapeutic effect. Physiological and histological kidney parameters were determined. MDSPCs exhibited similar immunophenotype, stem cell gene expression, and multilineage differentiation capacities as BM-MSCs, but they demonstrated higher proliferation rate. Single intravenous MDSPCs injection accelerated functional and morphological kidney recovery, as reflected by significantly lower serum creatinine levels, renal injury score, higher urinary creatinine, and GFR levels. PKH-26-labeled MDSPCs were identified within renal cortex 1 and 2 weeks after cell administration, indicating MDSPCs capacity to migrate and populate renal tissue. In conclusion, MDSPCs are capable of mediating functional and histological kidney recovery and can be considered as potential strategy for AKI treatment.
authors.affiliation: Institute of Physiology and Pharmacology, Medical Academy, Lithuanian University of Health Sciences, LT-44307 Kaunas, Lithuania.
authors.forename: Egle
authors.initials: E
authors.lastname: Pavyde
authors.affiliation: Institute of Physiology and Pharmacology, Medical Academy, Lithuanian University of Health Sciences, LT-44307 Kaunas, Lithuania; Department of Nephrology, Medical Academy, Lithuanian University of Health Sciences, LT-50009 Kaunas, Lithuania.
authors.forename: Romaldas
authors.initials: R
authors.lastname: Maciulaitis
authors.affiliation: State Research Institute Centre for Innovative Medicine, Vilnius University, LT-01102 Vilnius, Lithuania.
authors.forename: Mykolas
authors.initials: M
authors.lastname: Mauricas
authors.affiliation: State Research Institute Centre for Innovative Medicine, Vilnius University, LT-01102 Vilnius, Lithuania.
authors.forename: Gintaras
authors.initials: G
authors.lastname: Sudzius
authors.affiliation: National Center of Pathology, Vilnius University Hospital Santariskiu Clinics, LT-08406 Vilnius, Lithuania.
authors.forename: Ernesta
authors.initials: E
authors.lastname: Ivanauskaite Didziokiene
authors.affiliation: National Center of Pathology, Vilnius University Hospital Santariskiu Clinics, LT-08406 Vilnius, Lithuania; Department of Pathology, Forensic Medicine and Pharmacology, Faculty of Medicine, Vilnius University, LT-03101 Vilnius, Lithuania.
authors.forename: Arvydas
authors.initials: A
authors.lastname: Laurinavicius
authors.affiliation: Department of Non-Infectious Diseases, Veterinary Academy, Lithuanian University of Health Sciences, LT-47181 Kaunas, Lithuania.
authors.forename: Neringa
authors.initials: N
authors.lastname: Sutkeviciene
authors.affiliation: Institute of Physiology and Pharmacology, Medical Academy, Lithuanian University of Health Sciences, LT-44307 Kaunas, Lithuania.
authors.forename: Edgaras
authors.initials: E
authors.lastname: Stankevicius
authors.affiliation: Institute of Physiology and Pharmacology, Medical Academy, Lithuanian University of Health Sciences, LT-44307 Kaunas, Lithuania; Institute of Sports, Medical Academy, Lithuanian University of Health Sciences, LT-50275 Kaunas, Lithuania.
authors.forename: Justinas
authors.initials: J
authors.lastname: Maciulaitis
authors.affiliation: Institute of Physiology and Pharmacology, Medical Academy, Lithuanian University of Health Sciences, LT-44307 Kaunas, Lithuania.
authors.forename: Arvydas
authors.initials: A
authors.lastname: Usas