title: Autoantibodies against TYMS and PDLIM1 proteins detected as circulatory signatures in Indian breast cancer patients.
abstract: Breast cancer (BC) is the most common invasive cancer in women worldwide. Autoantibodies (AAbs) to tumor-associated antigens (TAAs) have a great potential for the development of diagnostic biomarkers in cancer. This study was performed to identify AAbs and cognate TAAs that may improve detection of this deadly disease.
  
  
  
  Serological proteome analysis of plasma samples of BC patients (N = 30) and healthy controls (N = 30) was performed to identify TAAs. Expressions of selected TAAs were also determined in breast tumor tissues (N = 10) by immunohistochemistry. An independent validation cohort (N = 124) was tested to determine diagnostic accuracy of selected AAbs titer by ELISA.
  
  
  
  Thymidylate synthase (TYMS) and C-terminal LIM domain protein 1 (PDLIM1) were found to react more specifically with plasma samples of BC patients. Both TAAs were also found to be significantly over expressed (p < 0.001) in breast tumor tissues compared to adjacent normal tissues. TYMS AAbs response was positively correlated (r = 0.778, p < 0.008) with TYMS overexpression in BC tissues. TYMS and PDLIM1 AAbs titers discriminated BC from controls with a sensitivity/specificity of 57.81%/95% and 73.44%/58.33%, respectively.
  
  
  
  High titers of both TYMS and PDLIM1 AAbs were significantly more prevalent in BC cases than controls. Our data recommends further investigations for evaluating their potential for BC detection.
authors.affiliation: Proteomics and Environment Carcinogenesis Laboratory, Food, Drug and Chemical Toxicology, Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Lucknow, Uttar Pradesh, India.|Academy of Scientific and Innovative Research (AcSIR), CSIR-IITR Campus, Lucknow, Uttar Pradesh, India.
authors.forename: Prachi
authors.initials: P
authors.lastname: Gupta
authors.affiliation: Proteomics and Environment Carcinogenesis Laboratory, Food, Drug and Chemical Toxicology, Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Lucknow, Uttar Pradesh, India.|Academy of Scientific and Innovative Research (AcSIR), CSIR-IITR Campus, Lucknow, Uttar Pradesh, India.
authors.forename: Shankar
authors.initials: S
authors.lastname: Suman
authors.affiliation: Plant Molecular Biology Laboratory, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India.|Academy of Scientific and Innovative Research (AcSIR), CSIR-IITR Campus, Lucknow, Uttar Pradesh, India.
authors.forename: Manisha
authors.initials: M
authors.lastname: Mishra
authors.affiliation: Proteomics and Environment Carcinogenesis Laboratory, Food, Drug and Chemical Toxicology, Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Lucknow, Uttar Pradesh, India.|Academy of Scientific and Innovative Research (AcSIR), CSIR-IITR Campus, Lucknow, Uttar Pradesh, India.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Mishra
authors.affiliation: Environment Toxicology Laboratory, CSIR-Indian Institute of Toxicology Research, Lucknow, Uttar Pradesh, India.|Academy of Scientific and Innovative Research (AcSIR), CSIR-IITR Campus, Lucknow, Uttar Pradesh, India.
authors.forename: Nidhi
authors.initials: N
authors.lastname: Srivastava
authors.affiliation: Department of Surgical Oncology, King George's Medical University, Lucknow, Uttar Pradesh, India.
authors.forename: Vijay
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Plant Molecular Biology Laboratory, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India.
authors.forename: Pradhyumna Kumar
authors.initials: PK
authors.lastname: Singh
authors.affiliation: Proteomics and Environment Carcinogenesis Laboratory, Food, Drug and Chemical Toxicology, Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Lucknow, Uttar Pradesh, India.
authors.forename: Yogeshwer
authors.initials: Y
authors.lastname: Shukla
mesh_terms: D000328:Adult; D000951:Antigens, Neoplasm / Q000097:blood* / Q000276:immunology; D001323:Autoantibodies / Q000097:blood* / Q000235:genetics; D014408:Biomarkers, Tumor / Q000097:blood* / Q000276:immunology; D001943:Breast Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D016022:Case-Control Studies; D042241:Early Diagnosis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007194:India; D060588:LIM Domain Proteins / Q000097:blood* / Q000276:immunology; D008875:Middle Aged; D012680:Sensitivity and Specificity; D013940:Thymidylate Synthase / Q000097:blood* / Q000276:immunology; D014157:Transcription Factors / Q000097:blood* / Q000276:immunology; D059467:Transcriptome / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm; D001323:Autoantibodies; D014408:Biomarkers, Tumor; C484572:LDB2 protein, human; D060588:LIM Domain Proteins; D014157:Transcription Factors; C000600377:TYMS protein, human; D013940:Thymidylate Synthase
keywords: Autoantibodies; Autoantigens; Breast cancer; PDLIM1; TYMS