title: Evaluation of soluble glycoprotein V as an in vitro quality marker for platelet concentrates: a correlation study between in vitro platelet quality markers and the effect of storage medium.
abstract: OBJECTIVE
  Extent of shape change (ESC), hypotonic shock response (HSR), the adhesive glycoprotein CD62P and lactate have been widely used as in vitro quality makers of platelet concentrates. Our aim was to evaluate soluble glycoprotein V (sGPV) as a platelet in vitro activation marker for platelet concentrates. Data were obtained from different validations during a twelve-year period.
  
  
  METHODS
  Platelet concentrates stored in PAS B, PAS E with 20-35% plasma carry-over or in plasma were prepared either from buffy coats or collected by apheresis. The samples were analysed up to day seven using sGPV, ESC, HSR, CD62P and lactate in addition to normal quality control assays.
  
  
  RESULTS
  sGPV correlated statistically significantly with ESC, HSR, CD62P and lactate in platelet concentrates stored in PASs.
  
  
  CONCLUSIONS
  We propose that quantitative sGPV which is calculated per platelet number could be used as the first choice in vitro platelet activation marker in validations and monitoring platelet production processes. sGPV could also be a promising alternative to platelet activation assays used in interlaboratory comparisons of the quality of platelet concentrates. Quantitative lactate and lactate production rate can be used to evaluate the quality of platelets concentrates during storage. However, the formation of lactate depends on the platelet count and the amount of glucose in the product making the comparison more difficult.
authors.affiliation: Finnish Red Cross Blood Service, Helsinki, Finland.
authors.forename: L K M
authors.initials: LK
authors.lastname: Kiminkinen
authors.affiliation: Finnish Red Cross Blood Service, Helsinki, Finland.
authors.forename: T
authors.initials: T
authors.lastname: Krusius
authors.affiliation: Finnish Red Cross Blood Service, Helsinki, Finland.
authors.forename: K M
authors.initials: KM
authors.lastname: Javela
mesh_terms: D015415:Biomarkers / Q000097:blood*; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism*; D001793:Blood Preservation; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D019344:Lactic Acid / Q000097:blood; D009997:Osmotic Pressure; D019007:P-Selectin / Q000097:blood; D015539:Platelet Activation; D010976:Platelet Count; D010980:Platelet Membrane Glycoproteins / Q000032:analysis
chemical_list: D015415:Biomarkers; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; C546937:SELP protein, human; D019344:Lactic Acid
keywords: CD62P; ESC; HSR; lactate; platelet concentrate; sGPV