title: Accelerating full thickness wound healing using collagen sponge of mrigal fish (Cirrhinus cirrhosus) scale origin.
abstract: The potentiality of collagen sponge as a skin substitute, derived from mrigal (Cirrhinus cirrhosus) scale has been explored in this study. Acid soluble collagen (ASC) and pepsin soluble collagen (PSC) from the scale of mrigal were isolated and characterized. The yields of ASC and PSC were ∼3% and ∼7% based on the dry weight of scale while the hydroxyproline content was ∼90mg/g. Scanning electron microscope revealed progressive demineralization with EDTA on time dependent scale. Further, the D-Spacing in fibril bundles were calculated to be ∼67nm. Fourier transform infrared and circular dichroism spectra confirmed extracted protein to be collagen I, where both ASC and PSC comprised of two different α-chains (α1and α2). The denaturation temperature (Td) of the collagen solution was 35°C closer to Tdof mammalian collagen. In vitro cell culture studies on the extracted collagen sponge showed efficient cell growth and proliferation. Additionally, co-culture with fibroblast and keratinocyte cells showed development of stratified epidermal layer in vitro. Faster wound healing potential of the extracted collagen in a rat model proved its applicability as a dermal substitute.
authors.affiliation: Biomaterilas and Tissue Engineering Laboratory, School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
authors.forename: Pallabi
authors.initials: P
authors.lastname: Pal
authors.affiliation: Biomaterilas and Tissue Engineering Laboratory, School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
authors.forename: Pavan Kumar
authors.initials: PK
authors.lastname: Srivas
authors.affiliation: Biomaterilas and Tissue Engineering Laboratory, School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
authors.forename: Prabhash
authors.initials: P
authors.lastname: Dadhich
authors.affiliation: Biomaterilas and Tissue Engineering Laboratory, School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
authors.forename: Bodhisatwa
authors.initials: B
authors.lastname: Das
authors.affiliation: Biomaterilas and Tissue Engineering Laboratory, School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
authors.forename: Priti Prasana
authors.initials: PP
authors.lastname: Maity
authors.affiliation: Department of Surgery, Bankura Sammilani Medical College, Bankura, India.
authors.forename: Dhrubajyoti
authors.initials: D
authors.lastname: Moulik
authors.affiliation: Biomaterilas and Tissue Engineering Laboratory, School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India. Electronic address: sdhara@smst.iitkgp.ernet.in.
authors.forename: Santanu
authors.initials: S
authors.lastname: Dhara
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002478:Cells, Cultured; D002675:Child, Preschool; D003094:Collagen / Q000737:chemistry*; D003530:Cyprinidae; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008422:Materials Testing; D019736:Prostheses and Implants; D017208:Rats, Wistar; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D003094:Collagen
keywords: Mrigal fish scale; RT-PCR; Type I collagen; Wound healing