title: Oral subchronic exposure to silver nanoparticles in rats.
abstract: Because of their extremely small size, silver nanoparticles (AgNPs) show unique physical and chemical properties, with specific biological effects, which make them particularly attractive for being used in a number of consumer applications. However, these properties also influence the potential toxicity of AgNPs. In this study, we assessed the potential toxic effects of an in vivo oral sub-chronic exposure to polyvinyl pyrrolidone coated AgNPs (PVP-AgNPs) in adult male rats. We also assessed if oral PVP-AgNPs exposure could alter the levels of various metals (Fe, Mg, Zn and Cu) in tissues. Rats were orally given 0, 50, 100 and 200 mg/kg/day of PVP-AgNPs. Silver (Ag) accumulation in tissues, Ag excretion, biochemical and hematological parameters, metal levels, as well as histopathological changes and subcellular distribution following PVP-AgNPs exposure, were also investigated. After 90 days of treatment, AgNPs were found within hepatic and ileum cells. The major tissue concentration of Ag was found in ileum of treated animals. However, all tissues of PVP-AgNPs-exposed animals showed increased levels of Ag in comparison with those of rats in the control group. No harmful effects in liver and kidney, as well as in biochemical markers were noted at any treatment dose. In addition, no hematological or histopathological changes were found in treated animals. However, significant differences in Cu and Zn levels were found in thymus and brain of PVP-AgNPs-treated rats.
authors.affiliation: Laboratory of Toxicology and Environmental Health, School of Medicine, IISPV, "Rovira i Virgili" University, Reus, Catalonia, Spain; Biochemistry Unit, School of Medicine, IISPV, "Rovira i Virgili" University, Reus, Catalonia, Spain.
authors.forename: Tania
authors.initials: T
authors.lastname: Garcia
authors.affiliation: Laboratory of Toxicology and Environmental Health, School of Medicine, IISPV, "Rovira i Virgili" University, Reus, Catalonia, Spain; Physiology Unit, School of Medicine, IISPV, "Rovira i Virgili" University, Reus, Catalonia, Spain.
authors.forename: Daisy
authors.initials: D
authors.lastname: Lafuente
authors.affiliation: Laboratory of Toxicology and Environmental Health, School of Medicine, IISPV, "Rovira i Virgili" University, Reus, Catalonia, Spain; Physiology Unit, School of Medicine, IISPV, "Rovira i Virgili" University, Reus, Catalonia, Spain.
authors.forename: Jordi
authors.initials: J
authors.lastname: Blanco
authors.affiliation: Laboratory of Toxicology and Environmental Health, School of Medicine, IISPV, "Rovira i Virgili" University, Reus, Catalonia, Spain; Physiology Unit, School of Medicine, IISPV, "Rovira i Virgili" University, Reus, Catalonia, Spain.
authors.forename: Domènec J
authors.initials: DJ
authors.lastname: Sánchez
authors.affiliation: Department of Pathology, University Hospital Joan XXIII, Tarragona, Catalonia, Spain.
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Sirvent
authors.affiliation: Laboratory of Toxicology and Environmental Health, School of Medicine, IISPV, "Rovira i Virgili" University, Reus, Catalonia, Spain.
authors.forename: José L
authors.initials: JL
authors.lastname: Domingo
authors.affiliation: Laboratory of Toxicology and Environmental Health, School of Medicine, IISPV, "Rovira i Virgili" University, Reus, Catalonia, Spain; Biochemistry Unit, School of Medicine, IISPV, "Rovira i Virgili" University, Reus, Catalonia, Spain. Electronic address: mariamercedes.gomez@urv.cat.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Gómez
mesh_terms: D000284:Administration, Oral; D000818:Animals; D008297:Male; D053768:Metal Nanoparticles / Q000008:administration & dosage*; D010592:Pharmaceutic Aids / Q000737:chemistry / Q000493:pharmacokinetics / Q000633:toxicity; D011205:Povidone / Q000737:chemistry* / Q000493:pharmacokinetics* / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D012834:Silver / Q000737:chemistry*; D014018:Tissue Distribution; D059550:Toxicity Tests, Subchronic / Q000379:methods*
chemical_list: D010592:Pharmaceutic Aids; D012834:Silver; D011205:Povidone
keywords: Metals; Oral exposure; Rats; Silver nanoparticles