title: Direct growth inhibition assay of total airborne fungi with application of biocide-treated malt extract agar.
abstract: Indoor air pollution by airborne fungi has risen to become a common issue all over the world and it is hazardous to indoor occupants' health as it is associated with a series of respiratory-related and skin-related diseases. Selected bioactive compounds from the food industry have been suggested to be effective against individual fungus isolated from indoor environment. However, the techniques used to evaluate these compounds were lengthy and unsuitable against total airborne fungi. Therefore, this paper describes an assay to assess the effectiveness of a bioactive compound to inhibit growth of total airborne fungi.•A combination and modification of previous methods and the NIOSH Manual Analytical Standard Method (NMAM 0800) is proposed.•This method concurrently samples the total airborne fungi and evaluates the ability of bioactive compounds (potassium sorbate in this paper), as a biocide, to treat these indoor airborne fungi.•The current method shortens the time of evaluation from 30 days to only 5 days and employs the counting of colony forming units (CFUs) to ease the measurement of the growth of fungi.
authors.affiliation: Department of Water and Environmental Engineering (DWEE), Faculty of Civil and Environmental Engineering (FKAAS), Universiti Tun Hussein Onn Malaysia, 86400 Parit Raja, Batu Pahat, Johor, Malaysia.
authors.forename: Chin Ming
authors.initials: CM
authors.lastname: Er
authors.affiliation: Department of Chemical Engineering Technology, Faculty of Engineering Technology (FTK), Universiti Tun Hussein Onn Malaysia, 86400 Parit Raja, Batu Pahat, Johor, Malaysia.
authors.forename: N M
authors.initials: NM
authors.lastname: Sunar
authors.affiliation: Department of Chemical Engineering Technology, Faculty of Engineering Technology (FTK), Universiti Tun Hussein Onn Malaysia, 86400 Parit Raja, Batu Pahat, Johor, Malaysia.
authors.forename: A M
authors.initials: AM
authors.lastname: Leman
authors.affiliation: Department of Water and Environmental Engineering (DWEE), Faculty of Civil and Environmental Engineering (FKAAS), Universiti Tun Hussein Onn Malaysia, 86400 Parit Raja, Batu Pahat, Johor, Malaysia.
authors.forename: N
authors.initials: N
authors.lastname: Othman
keywords: Airborne fungi contamination; Biocide; Direct growth inhibition assay of airborne fungi; Fungal growth inhibition; Indoor air quality; NMAM 0800; Potassium sorbate