title: Synthesis of silver nanoparticles by Bacillus subtilis T-1 growing on agro-industrial wastes and producing biosurfactant.
abstract: In this study, culture supernatnats of Bacillus subtilis T-1 growing on brewery effluents and molasses was used for silver nanoparticles (Ag-NPs) synthesis. The biosurfactant production of B. subtilis T-1 was confirmed by the detection of genes in the genome and by the identification of the product in the supernatants. The genes for synthesis of surfactin (sfp, srfAA) and iturin (ituC) were noted by PCR reactions. Also, in examined culture supernatants the presence of C13, C14 and C15 surfactin homologues with the sodiated molecules [M + Na](+) at m/z 1030, 1044 and 1058 was confirmed using LC/MS/MS analysis. The formation of NPs in the culture supernatants was confirmed by UV-vis spectroscopy. The dynamic light scattering measurements and transmission electron microscopy images showed the nanometric sizes of the biosynthesised Ag-NPs which ranged from several nm to several tens of nm depending on the used culture supernatant. Biological properties of Ag-NPs were evaluated by binding of Ag-NPs with DNA isolated from the Escherichia coli ATCC 25922 and B. subtilis ATCC 6633. Biogenic Ag-NPs were actively bound to DNA in increased concentration which could be the one important mode of antibacterial action of the Ag-NPs.
authors.affiliation: Department of Environmental Microbiology, Institute for Ecology of Industrial Areas, 6 Kossutha, 40-844 Katowice, Poland. pla@ietu.katowice.pl.
authors.forename: Grażyna Anna
authors.initials: GA
authors.lastname: Płaza
authors.affiliation: Department of Environmental Microbiology, Institute for Ecology of Industrial Areas, 6 Kossutha, 40-844 Katowice, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Chojniak
authors.affiliation: Centre of Polymer and Carbon Materials, Polish Academy of Sciences, M. Curie-Sklodowskiej 34, 41-819 Zabrze, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Mendrek
authors.affiliation: Centre of Polymer and Carbon Materials, Polish Academy of Sciences, M. Curie-Sklodowskiej 34, 41-819 Zabrze, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Trzebicka
authors.affiliation: Department of Physical Chemistry, Faculty of Science, Regional Centre of Advanced Technologies and Materials, Palacky University in Olomouc 17, Listopadu 12, 771 46 Olomouc, Czech Republic.
authors.forename: Libor
authors.initials: L
authors.lastname: Kvitek
authors.affiliation: Department of Physical Chemistry, Faculty of Science, Regional Centre of Advanced Technologies and Materials, Palacky University in Olomouc 17, Listopadu 12, 771 46 Olomouc, Czech Republic.
authors.forename: Ales
authors.initials: A
authors.lastname: Panacek
authors.affiliation: Department of Physical Chemistry, Faculty of Science, Regional Centre of Advanced Technologies and Materials, Palacky University in Olomouc 17, Listopadu 12, 771 46 Olomouc, Czech Republic.
authors.forename: Robert
authors.initials: R
authors.lastname: Prucek
authors.affiliation: Department of Physical Chemistry, Faculty of Science, Regional Centre of Advanced Technologies and Materials, Palacky University in Olomouc 17, Listopadu 12, 771 46 Olomouc, Czech Republic.
authors.forename: Radek
authors.initials: R
authors.lastname: Zboril
authors.affiliation: Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Lodz, 12/16 Banacha, 90-237 Lodz, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Paraszkiewicz
authors.affiliation: Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Lodz, 12/16 Banacha, 90-237 Lodz, Poland.
authors.forename: Przemysław
authors.initials: P
authors.lastname: Bernat
mesh_terms: D000383:Agriculture; D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D007220:Industrial Waste; D055666:Lipopeptides / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D008965:Molasses / Q000382:microbiology; D010316:Particle Size; D010456:Peptides, Cyclic / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012834:Silver / Q000737:chemistry* / Q000378:metabolism*; D013501:Surface-Active Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D007220:Industrial Waste; D055666:Lipopeptides; D010456:Peptides, Cyclic; D013501:Surface-Active Agents; C009365:surfactin peptide; D012834:Silver; C033468:iturin C