title: SOD3 Ameliorates H2O2-Induced Oxidative Damage in SH-SY5Y Cells by Inhibiting the Mitochondrial Pathway.
abstract: This study was designed to investigate the protective effects of extracellular superoxide dismutase (SOD3) against hydrogen peroxide (H2O2) induced damage in human neuroblastoma SH-SY5Y cells and to elucidate the mechanisms responsible for this beneficial effect. SOD3-overexpressing SH-SY5Y cells were generated by adenoviral vector-mediated infection, and H2O2 was then added into the cell culture system to establish an in vitro model of oxidative stress. Cell viability, the generation of intracellular reactive oxygen species (ROS), the expression and activity of antioxidant enzymes, the levels of lipid peroxidation malondialdehyde (MDA), the expression of mitochondrial apoptosis-related genes, and calcium imaging were examined. Following H2O2 exposure, the over-expression of SOD3 promoted the survival of SH-SY5Y cells; decreased the production of ROS, MDA levels, cytochrome C, caspase-3, caspase-9, and Bax gene expression, and calcium levels; and increased the expression and activity of antioxidant enzyme genes and the expression level of Bcl-2. Together, our data demonstrate that SOD3 ameliorates H2O2-induced oxidative damage in neuroblastoma SH-SY5Y cells by inhibiting the mitochondrial pathway and provide new insights into the functional actions of SOD3 on oxidative stress-induced cell damage.
authors.affiliation: Research Center of Neuroscience, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Yang
authors.affiliation: Key Laboratory of Birth Defects and Reproductive Health of the National Health and Family Planning Commission, Chongqing Population and Family Planning Science and Technology Research Institute, Chongqing, 400020, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wei
authors.affiliation: Research Center of Neuroscience, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Fu
authors.affiliation: Department of Anesthesiology, University Hospital of Hubei University for Nationalities, Enshi, 445000, Hubei, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.affiliation: Affiliated Hospital of the Academy of Military Medical Sciences, Beijing, 100071, China. youhua307@163.com.
authors.forename: Hua
authors.initials: H
authors.lastname: You
authors.affiliation: Research Center of Neuroscience, Chongqing Medical University, Chongqing, 400016, China. huarongyu2003@163.com.
authors.forename: Hua-Rong
authors.initials: HR
authors.lastname: Yu
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D013482:Superoxide Dismutase / Q000096:biosynthesis*
chemical_list: D006861:Hydrogen Peroxide; C441642:SOD3 protein, human; D013482:Superoxide Dismutase
keywords: Antioxidant enzymes; Extracellular superoxide dismutase; Mitochondrial pathway; Oxidative stress; Reactive oxygen species