title: Differential expression of TYRP1 in adult human retinal pigment epithelium and uveal melanoma cells.
abstract: Uveal melanoma (UM) is the most frequently occurring primary intraocular malignancy in adults. Tyrosinase (TYR) is a copper-containing enzyme and a type I membrane protein that is involved in the generation of melanin, the main pigment in vertebrates. TYR-related protein 1 (TYRP1) is regarded to have a crucial role in the immunotherapy of melanoma. As biomarkers, the TYR-related proteins, TYRP1 and TYRP2, exhibit specific expression in melanocytes, while also contributing to melanin synthesis within melanosomes. In the present study, the differential expression of TYRP1 was investigated at the mRNA, protein and morphological levels in four human UM cell lines (SP6.5, OM431, OCM1 and OCM290) and the human retinal pigment epithelium (RPE) cell line, using polymerase chain reaction, western blotting, immunocytochemistry and immunofluorescence staining. It was found that SP6.5 cells expressed the highest level of TYRP1, in comparison to SP6.5 OCM1 and OM431 cells, which produced less TYRP1, and OCM290 cells, which produced almost no TYRP1. No TYRP1 protein expression was identified in the RPE cell line. These findings indicate the potential use of TYRP1 in the development of therapy for UM.
authors.affiliation: School of Life Science, Anhui University, Hefei, Anhui 230601, P.R. China; Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200011, P.R. China.
authors.forename: Chun
authors.initials: C
authors.lastname: Qiu
authors.affiliation: School of Life Science, Anhui University, Hefei, Anhui 230601, P.R. China; Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200011, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200011, P.R. China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Bi
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200011, P.R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200011, P.R. China.
authors.forename: Linna
authors.initials: L
authors.lastname: Lu
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200011, P.R. China.
authors.forename: Guanxiang
authors.initials: G
authors.lastname: Qian
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200011, P.R. China.
authors.forename: Renbing
authors.initials: R
authors.lastname: Jia
authors.affiliation: School of Life Science, Anhui University, Hefei, Anhui 230601, P.R. China.
authors.forename: Rong
authors.initials: R
authors.lastname: Jia
keywords: TYR-related protein 1; differential expression; immunocytochemistry; immunofluorescence; uveal melanoma