title: Influence of Culture Media on Detection of Carbapenem Hydrolysis by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.
abstract: In this study, we evaluated the influence of distinct bacterial growth media on detection of carbapenemase hydrolysis by matrix-assisted laser desorption ionization-time of flight mass spectrometry. False-negative results were observed for OXA-25-, OXA-26-, and OXA-72-producing Acinetobacter baumannii isolates grown on MacConkey agar medium. The other culture media showed 100% sensitivity and 100% specificity for detecting carbapenemase.
authors.affiliation: Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina/Universidade Federal de São Paulo, São Paulo, SP, Brazil anarolramos@msn.com.
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Ramos
authors.affiliation: Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina/Universidade Federal de São Paulo, São Paulo, SP, Brazil.|Laboratório Central, Hospital São Paulo, UNIFESP, São Paulo, SP, Brazil.
authors.forename: Cecília Godoy
authors.initials: CG
authors.lastname: Carvalhaes
authors.affiliation: Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina/Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Jhonatha Rodrigo
authors.initials: JR
authors.lastname: Cordeiro-Moura
authors.affiliation: bioMérieux, São Paulo, SP, Brazil.
authors.forename: Anna Carolina
authors.initials: AC
authors.lastname: Rockstroh
authors.affiliation: Laboratório Central, Hospital São Paulo, UNIFESP, São Paulo, SP, Brazil.
authors.forename: Antonia Maria Oliveira
authors.initials: AMO
authors.lastname: Machado
authors.affiliation: Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina/Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Gales
mesh_terms: D040981:Acinetobacter baumannii / Q000201:enzymology*; D001426:Bacterial Proteins / Q000032:analysis*; D015780:Carbapenems / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry*; D005188:False Negative Reactions; D006868:Hydrolysis; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins; D015780:Carbapenems; D003470:Culture Media; D001618:beta-Lactamases; C063912:carbapenemase