title: MicroRNA-1976 functions as a tumor suppressor and serves as a prognostic indicator in non-small cell lung cancer by directly targeting PLCE1.
abstract: Lung cancer remains a leading cause of cancer-associated mortality worldwide and non-small lung cancer (NSCLC) is responsible for over 80% of lung cancer-related deaths. Identifying novel molecular biomarker that can inhibit the progression of lung cancer will facilitate the development of new treatment strategies. Phospholipase C epsilon 1 (PLCE1) is a susceptibility gene in NSCLC. Nevertheless, the role of PLCE1 in NSCLC tumorigenesis has not been elucidated. Herein, we demonstrated that miR-1976 is a tumor-suppressor miRNA in NSCLC progression. We found that expression of miR-1976 was decreased obviously in NSCLC tissues. Down-regulation of miR-1976 was associated with TNM stage and postoperative survival. Overexpression of miR-1976 inhibited the growth and metastasis of NSCLC cells. The PLCE1 was a direct target of miR-1976 and promoted the growth and metastasis of NSCLC cells. Further study showed that PLCE1 could reverse the inhibitory effect of miR-1976 on growth and metastasis of NSCLC cells. Taken together, our data highlight the pivotal role of miR-1976 in the progression of NSCLC. Thus, miR-1976 may be a potential prognostic marker and of treatment relevance for NSCLC progression intervention.
authors.affiliation: Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan, 430071, PR China. Electronic address: gangchenfly@sina.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan, 430071, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan, 430071, PR China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan, 430071, PR China.
authors.forename: Lijing
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan, 430071, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Chen
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000401:mortality*; D002681:China / Q000453:epidemiology; D016147:Genes, Tumor Suppressor; D005819:Genetic Markers / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000453:epidemiology / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000401:mortality*; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D054801:Phosphoinositide Phospholipase C / Q000235:genetics*; D015995:Prevalence; D011379:Prognosis; D018570:Risk Assessment; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; D005819:Genetic Markers; C000618452:MIRN-1976 microRNA, human; D035683:MicroRNAs; D054801:Phosphoinositide Phospholipase C; C423320:phospholipase C epsilon
keywords: Apoptosis; Hsa-miRNA-1976 (miR-1976); Non-small cell lung cancer (NSCLC); PLCE1; Proliferation