title: Effect of simulated air dive and decompression sickness on the plasma proteome of rats.
abstract: Decompression sickness (DCS) is a poorly understood systemic disease caused by inadequate desaturation following a reduction in ambient pressure. Although recent studies highlight the importance of circulating factors, the available data are still puzzling. In this study, we aimed to identify proteins and biological pathways involved in the development of DCS in rats.
  
  
  
  Eighteen male Sprague-Dawley rats were subjected to a same simulated air dive to 1000 kPa absolute pressure and divided into two groups: no DCS or DCS. A third control group remained at atmospheric pressure. Venous blood was collected after hyperbaric exposure and the plasma proteomes from four individuals per group were analyzed by using a two-dimensional electrophoresis-based proteomic strategy.
  
  
  
  Quantitative analysis identified nine protein spots with abundances significantly changed (false discovery rate < 0.1) between the tested conditions. Three protein spots, identified as Apolipoprotein A1, Serine Protease Inhibitor A3K (Serpin A3K), and Alpha-1-antiproteinase, appeared increased in DCS animals but displayed only weak changes. By contrast, one protein spot identified as Transthyretin (TTR) dramatically decreased (i.e. quite disappeared) in animals displaying DCS symptoms. Before diving, TTR level was not different in DCS than nondiving group.
  
  
  
  These results may lead to the use of TTR as an early biomarker of DCS.
authors.affiliation: Laboratoire ORPHY EA 4324, Université de Bretagne Occidentale, Brest, France.
authors.forename: Jacky
authors.initials: J
authors.lastname: Lautridou
authors.affiliation: LEMAR UMR 6539 CNRS/UBO/IRD/Ifremer, Université de Bretagne Occidentale, Brest, France.
authors.forename: Vianney
authors.initials: V
authors.lastname: Pichereau
authors.affiliation: LEMAR UMR 6539 CNRS/UBO/IRD/Ifremer, Université de Bretagne Occidentale, Brest, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Artigaud
authors.affiliation: Laboratoire ORPHY EA 4324, Université de Bretagne Occidentale, Brest, France.
authors.forename: Peter
authors.initials: P
authors.lastname: Buzzacott
authors.affiliation: Laboratoire ORPHY EA 4324, Université de Bretagne Occidentale, Brest, France.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Proteogen SFR ICORE 4206, Université de Caen, Caen, France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Bernay
authors.affiliation: Laboratoire ORPHY EA 4324, Université de Bretagne Occidentale, Brest, France.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Driad
authors.affiliation: Laboratoire ORPHY EA 4324, Université de Bretagne Occidentale, Brest, France.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Mazur
authors.affiliation: Laboratoire ORPHY EA 4324, Université de Bretagne Occidentale, Brest, France.
authors.forename: Kate
authors.initials: K
authors.lastname: Lambrechts
authors.affiliation: Laboratoire ORPHY EA 4324, Université de Bretagne Occidentale, Brest, France.
authors.forename: Michael
authors.initials: M
authors.lastname: Théron
authors.affiliation: Laboratoire ORPHY EA 4324, Université de Bretagne Occidentale, Brest, France.
authors.forename: François
authors.initials: F
authors.lastname: Guerrero
mesh_terms: D000388:Air; D000818:Animals; D016632:Apolipoprotein A-I / Q000097:blood / Q000235:genetics; D015415:Biomarkers / Q000097:blood; D003665:Decompression Sickness / Q000097:blood* / Q000175:diagnosis* / Q000503:physiopathology; D004242:Diving; D042241:Early Diagnosis; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D011228:Prealbumin / Q000235:genetics* / Q000378:metabolism; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000514:alpha 1-Antichymotrypsin / Q000097:blood / Q000235:genetics; D000515:alpha 1-Antitrypsin / Q000097:blood / Q000235:genetics
chemical_list: D016632:Apolipoprotein A-I; D015415:Biomarkers; D011228:Prealbumin; D020543:Proteome; D000514:alpha 1-Antichymotrypsin; D000515:alpha 1-Antitrypsin
keywords: Decompression sickness; Diving; Rat; Transthyretin