title: Rapid and Specific Screening Assay for KRAS Oncogene Mutation by a Novel Gene Amplification Method.
abstract: BACKGROUND
  Epidermal growth factor receptor (EGFR) is a target of molecular therapeutics for colorectal cancer. However, mutation of Kirsten rat sarcoma viral oncogene homolog (KRAS) gene at codons 12 and 13 attenuates the therapeutic effect of anti-EGFR therapies. Therefore, the detection of KRAS gene mutation is important for therapeutic decision-making.
  
  
  METHODS
  KRAS gene mutation at codons 12 (c.34G>T, c.35G>C, c.35G>A) and 13 (c.38G>A) in six cancer cell lines were investigated. A loop-mediated isothermal amplification-based procedure was developed that employed peptide nucleic acid to suppress amplification of the wild-type allele.
  
  
  RESULTS
  This mutation-oriented gene-amplification procedure can amplify the DNA fragment of the KRAS gene with codon 12 and codon 13 mutation within 30 min. Moreover, boiled cells can work as template resources.
  
  
  CONCLUSIONS
  This newly developed procedure can be useful for patient stratification for anti-EGFR therapies.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Tokyo Women's Medical University, School of Medicine, Shinjyuku-ku, Tokyo, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Kumasaka
authors.affiliation: Department of Pathology, Nihon University School of Dentistry, Chiyoda-ku, Tokyo, Japan matsumoto.naoyuki@nihon-u.ac.jp.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Matsumoto
authors.affiliation: Department of Pathology, Nihon University School of Dentistry, Chiyoda-ku, Tokyo, Japan.
authors.forename: Shotaro
authors.initials: S
authors.lastname: Mukae
authors.affiliation: Department of Pathology, Nihon University School of Dentistry, Chiyoda-ku, Tokyo, Japan.
authors.forename: Taiichi
authors.initials: T
authors.lastname: Kitano
authors.affiliation: Department of Oral and Maxillofacial Surgery, Nihon University School of Dentistry, Chiyoda-ku, Tokyo, Japan.
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Noguchi
authors.affiliation: Department of Oral and Maxillofacial Surgery, Nihon University School of Dentistry, Chiyoda-ku, Tokyo, Japan.
authors.forename: Hidero
authors.initials: H
authors.lastname: Ohki
authors.affiliation: Department of Pathology, Nihon University School of Dentistry, Chiyoda-ku, Tokyo, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Komiyama
authors.affiliation: Department of Oral and Maxillofacial Surgery, Tokyo Women's Medical University, School of Medicine, Shinjyuku-ku, Tokyo, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Ando
mesh_terms: D045744:Cell Line, Tumor; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics; D005784:Gene Amplification; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D009061:Mouth Mucosa / Q000166:cytology; D009154:Mutation; D021141:Nucleic Acid Amplification Techniques
chemical_list: D004247:DNA
keywords: KRAS mutation; LAMP; molecular targeted therapy; stratification biomarker