title: 4',6-dimethoxyisoflavone-7-O-β-D-glucopyranoside (wistin) is a peroxisome proliferator-activated receptor γ (PPARγ) agonist that stimulates adipocyte differentiation.
abstract: Peroxisome proliferator-activated receptor γ (PPARγ) is a ligand-dependent transcription factor that directly activates the expression of adipocyte-specific genes, and is universally accepted as the master regulator for adipocyte differentiation. Using a PPARγ luciferase reporter assay system, we showed that 4',6-dimethoxyisoflavone-7-O-β-D-glucopyranoside (wistin) dose-dependently activates PPARγ. Treatment with wistin enhanced the marker of adipocyte differentiation, such as triglyceride accumulation in 3T3-L1 cells. Real-time quantitative polymerase chain reaction showed that wistin increased the expression of PPARγ2 messenger RNA. Moreover, the addition of wistin upregulated the expression of PPARγ-target genes, aP2 and adiponectin in 3T3-L1 cells. To our knowledge, wistin is the first isoflavonoid O-glycoside that exhibits PPARγ agonist activity.
authors.affiliation: Graduate School of Bioscience, Nagahama Institute for Bioscience and Technology, Nagahama, Shiga, Japan.
authors.forename: Matoki
authors.initials: M
authors.lastname: Sanada
authors.affiliation: Graduate School of Bioscience, Nagahama Institute for Bioscience and Technology, Nagahama, Shiga, Japan.
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Hayashi
authors.affiliation: Graduate School of Bioscience, Nagahama Institute for Bioscience and Technology, Nagahama, Shiga, Japan.
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Imai
authors.affiliation: Graduate School of Bioscience, Nagahama Institute for Bioscience and Technology, Nagahama, Shiga, Japan.
authors.forename: Fumiya
authors.initials: F
authors.lastname: Nakamura
authors.affiliation: Graduate School of Bioscience, Nagahama Institute for Bioscience and Technology, Nagahama, Shiga, Japan.
authors.forename: Tomoyo
authors.initials: T
authors.lastname: Inoue
authors.affiliation: Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Ohta
authors.affiliation: Graduate School of Bioscience, Nagahama Institute for Bioscience and Technology, Nagahama, Shiga, Japan. hkawachi@nagahama-i-bio.ac.jp.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kawachi
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology*; D052242:Adiponectin / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D005960:Glucosides / Q000494:pharmacology*; D000882:Haplorhini; D007529:Isoflavones / Q000494:pharmacology*; D051379:Mice; D047495:PPAR gamma / Q000819:agonists* / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D013268:Stimulation, Chemical; D014280:Triglycerides / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: C000615693:4',6-dimethoxyisoflavone-7-O-beta-D-glucopyranoside; D052242:Adiponectin; C493411:Fabp4 protein, mouse; D050556:Fatty Acid-Binding Proteins; D005960:Glucosides; D007529:Isoflavones; D047495:PPAR gamma; D012333:RNA, Messenger; D014280:Triglycerides
keywords: PPARγ agonist; adipocyte differentiation; wistin