title: Metabolic engineering with systems biology tools to optimize production of prokaryotic secondary metabolites.
abstract: Covering: 2012 to 2016Metabolic engineering using systems biology tools is increasingly applied to overproduce secondary metabolites for their potential industrial production. In this Highlight, recent relevant metabolic engineering studies are analyzed with emphasis on host selection and engineering approaches for the optimal production of various prokaryotic secondary metabolites: native versus heterologous hosts (e.g., Escherichia coli) and rational versus random approaches. This comparative analysis is followed by discussions on systems biology tools deployed in optimizing the production of secondary metabolites. The potential contributions of additional systems biology tools are also discussed in the context of current challenges encountered during optimization of secondary metabolite production.
authors.affiliation: BioInformatics Research Center, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea. ehukim@kaist.ac.kr.
authors.forename: Hyun Uk
authors.initials: HU
authors.lastname: Kim
authors.forename: Pep
authors.initials: P
authors.lastname: Charusanti
authors.forename: Sang Yup
authors.initials: SY
authors.lastname: Lee
authors.forename: Tilmann
authors.initials: T
authors.lastname: Weber
mesh_terms: D001709:Biotechnology; D019295:Computational Biology; D004926:Escherichia coli / Q000378:metabolism; D005818:Genetic Engineering; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways; D008954:Models, Biological; D015394:Molecular Structure; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D049490:Systems Biology