title: Chemical characterization of Citrus limon var. pompia and incorporation in phospholipid vesicles for skin delivery.
abstract: The components of pompia, a hybrid Citrus species cultivated only in Sardinia (Italy), were extracted using an environmentally-friendly method and food-grade solvents. Taking into account that only few data are available on pompia composition, the phytochemical fingerprint of its rind extract was obtained by accurate component separation and identification, combining HPLC and mass spectrometry. Different flavones such as naringin (23.77μg/mg), neoeriocitrin (46.53μg/mg) and neohesperidin (44.57μg/mg) were identified. Additionally, the antioxidant activity and phenolic content were confirmed by DPPH and Folin-Ciocalteu assays. The whole extract was incorporated in innovative phospholipid vesicles, namely glycerosomes, hyalurosomes and glycerol containing hyalurosomes, which were prepared using a high ratio of extract/phospholipid (1/3.5w/w). The in vitro biocompatibility of the nanoincorporated extract and its ability to potentiate the aptitude of the extract to counteract oxidative stress in skin cells were evaluated. The vesicles, especially glycerol containing hyalurosomes, were able to prevent oxidative damage and death of both keratinocytes and fibroblasts, promoting their viability.
authors.affiliation: Dept. Scienze della Vita e dell'Ambiente, University of Cagliari, Cagliari, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Manconi
authors.affiliation: Dept. Scienze della Vita e dell'Ambiente, University of Cagliari, Cagliari, Italy. Electronic address: mlmanca@unica.it.
authors.forename: Maria Letizia
authors.initials: ML
authors.lastname: Manca
authors.affiliation: Dept. Scienze della Vita e dell'Ambiente, University of Cagliari, Cagliari, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Marongiu
authors.affiliation: Dept. Scienze della Vita e dell'Ambiente, University of Cagliari, Cagliari, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Caddeo
authors.affiliation: Dept. Scienze della Vita e dell'Ambiente, University of Cagliari, Cagliari, Italy.
authors.forename: Ines
authors.initials: I
authors.lastname: Castangia
authors.affiliation: Dept. of Chemistry and Pharmacy, University of Sassari, Sassari, Italy.
authors.forename: Giacomo Luigi
authors.initials: GL
authors.lastname: Petretto
authors.affiliation: Dept. of Chemistry and Pharmacy, University of Sassari, Sassari, Italy.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Pintore
authors.affiliation: Dept. Scienze della Vita e dell'Ambiente, University of Cagliari, Cagliari, Italy.
authors.forename: Giorgia
authors.initials: G
authors.lastname: Sarais
authors.affiliation: Institute of Science of Food Production UOS Sassari-CNR, Traversa la Crucca, 3 Loc. Baldinca, 07040 Sassari, Italy.
authors.forename: Guy
authors.initials: G
authors.lastname: D'hallewin
authors.affiliation: Icnoderm srl, Sardegna Ricerche Ed.5, Pula (Cagliari), 09010, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Zaru
authors.affiliation: Dept. Scienze della Vita e dell'Ambiente, University of Cagliari, Cagliari, Italy.
authors.forename: Gianluigi
authors.initials: G
authors.lastname: Bacchetta
authors.affiliation: Dept. Scienze della Vita e dell'Ambiente, University of Cagliari, Cagliari, Italy.
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Fadda
mesh_terms: D016475:3T3 Cells; D000279:Administration, Cutaneous; D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D002957:Citrus / Q000737:chemistry*; D016503:Drug Delivery Systems; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007558:Italy; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism; D013058:Mass Spectrometry; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000302:isolation & purification; D010743:Phospholipids / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D010636:Phenols; D010743:Phospholipids; D010936:Plant Extracts
keywords: Antioxidant; Glycerol; Liposomes; Plant extract; Skin cells; Sodium hyaluronate