title: Immunoproteomic Approach for Screening Vaccine Candidates from Bacterial Outer Membrane Proteins.
abstract: Outer membrane proteins (OMPs) are unique to Gram-negative bacteria and have been revealed as potential vaccine candidates for conferring protection against infections in recent years. Immunoproteomics is a powerful technique that is ideally suited to screen and identify potential vaccine candidates. This chapter presents a brief outline of the screening of immunogenic OMPs from Vibrio parahaemolyticus by an immunoproteomic strategy that was based on two-dimensional electrophoresis (2-DE) and immunoblotting. The protective efficacy provided by the immunogenic OMP Vp0802 determined by active protection experiment assays is also presented in brief.
authors.affiliation: Institute of Proteomics and Molecular Enzymology, School of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, China. jianyi.pan@zstu.edu.cn.
authors.forename: Jianyi
authors.initials: J
authors.lastname: Pan
authors.affiliation: Institute of Proteomics and Molecular Enzymology, School of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, China.
authors.forename: Chuchu
authors.initials: C
authors.lastname: Li
authors.affiliation: Institute of Proteomics and Molecular Enzymology, School of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, China.
authors.forename: Zhicang
authors.initials: Z
authors.lastname: Ye
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology* / Q000378:metabolism*; D001428:Bacterial Vaccines / Q000276:immunology* / Q000378:metabolism*; D007106:Immune Sera / Q000276:immunology; D051379:Mice; D008813:Mice, Inbred ICR; D040901:Proteomics / Q000379:methods*; D014736:Vibrio parahaemolyticus / Q000276:immunology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D007106:Immune Sera
keywords: Immunogenic; Immunoproteomics; Outer membrane protein (OMP); Protective antigen; V. parahaemolyticus; Vaccine candidate