title: Endothelial fibrinolytic response onto an evolving matrix of fibrin.
abstract: BACKGROUND
  Fibrin provides a temporary matrix at the site of vascular injury. The aims of the present work were (1) to follow fibrin formation and lysis onto the surface of human dermal microvascular endothelial cells (HMEC-1), and (2) to quantify the secretion of fibrinolytic components in the presence of fibrin.
  
  
  METHODS
  Fibrin clots at different fibrinogen concentrations were formed on top of (model 1) or beneath (model 2) the endothelial cells. Fibrin formation or lysis onto the surface of HMEC-1 cells, was followed by turbidity. Clot structure was visualized by laser scanning confocal microscopy (LSCM). The secretion of uPA and PAI-1 by HMEC-1 cells was quantified by ELISA.
  
  
  RESULTS
  The rate of fibrin formation increased approximately 1.5-fold at low fibrinogen content (0.5 and 1 mg/mL; p < 0.05) compared to the condition without cells; however, it was decreased at 2 mg/mL fibrinogen (p < 0.05) and no differences were found at higher fibrinogen concentrations (3 and 5 mg/mL). HMEC-1 retarded dissolution of clots formed onto their surface at 0.5 to 3 mg/mL fibrinogen (p < 0.05). Secretion of uPA was 13 × 10(-6) ng/mL per cell in the absence of RGD and 8 × 10(-6) ng/mL per cell in the presence of RGD, when clots were formed on the top of HMEC-1. However, the opposite was found when cells were grown over fibrin: 6 × 10(-6) ng/mL per cell without RGD vs. 17 × 10(-6) ng/mL per cell with RGD. The secretion of PAI-1 by HMEC-1 cells was unrelated to the presence of fibrin or RGD, 7 × 10(-6) μg/mL per cell and 5 × 10(-6) μg/mL per cell, for the apical (model 1) and basal clots (model 2), respectively.
  
  
  CONCLUSIONS
  HMEC-1 cells influence fibrin formation and dissolution as a function of the fibrin content of clots. Clot degradation was accentuated at high fibrin concentrations. The secretion of fibrinolytic components by HMEC-1 cells seemed to be modulated by integrins that bind RGD ligands.
authors.affiliation: Centro de Medicina Experimental, Laboratorio Biología del Desarrollo de la Hemostasia, Instituto Venezolano de Investigaciones Científicas, Caracas, República Bolivariana de Venezuela ; Universidad de Carabobo, Escuela de Bioanálisis (Sede Aragua), Maracay, República Bolivariana de Venezuela.
authors.forename: O
authors.initials: O
authors.lastname: Castillo
authors.affiliation: Instituto de Inmunología, Universidad Central de Venezuela, Caracas, República Bolivariana de Venezuela ; Laboratorio de Fisiología Celular, Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas, Caracas, República Bolivariana de Venezuela.
authors.forename: H
authors.initials: H
authors.lastname: Rojas
authors.affiliation: Instituto de Medicina Experimental, Universidad Central de Venezuela, Caracas, República Bolivariana de Venezuela.
authors.forename: Z
authors.initials: Z
authors.lastname: Domínguez
authors.affiliation: Inserm UMR_S 1140, Faculté de Pharmacie, Paris, France ; Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors.forename: E
authors.initials: E
authors.lastname: Anglés-Cano
authors.affiliation: Centro de Medicina Experimental, Laboratorio Biología del Desarrollo de la Hemostasia, Instituto Venezolano de Investigaciones Científicas, Caracas, República Bolivariana de Venezuela.
authors.forename: R
authors.initials: R
authors.lastname: Marchi
keywords: Endothelium; Fibrin; Fibrinogen; Fibrinolysis; Human dermal microvascular endothelial cells; Plasminogen activator inhibitor type 1; Urokinase-type plasminogen activator