title: Fabrication of a LRET-based upconverting hybrid nanocomposite for turn-on sensing of H2O2 and glucose.
abstract: Blood glucose detecting has aroused considerable attention because diabetes mellitus has become a worldwide publish health problem. Herein, we construct an exceptionally simple upconverting hybrid nanocomposite, composed of DNA-templated Ag nanoparticles (DNA-AgNPs) and NaYF4:Yb/Tm@NaYF4 core-shell upconversion nanoparticles (UCNPs), for the sensing of H2O2 and glucose. In this design, UCNPs with bared surface act as the donor, and DNA-AgNPs serve as efficient quenchers. DNA-AgNPs can be directly assembled on the bared surface of UCNPs, which further decreases the distance of donor-to-acceptor. The formation of DNA-AgNPs/UCNP nanocomposite results in luminescence quenching of UCNP by DNA-AgNPs through luminescence resonance energy transfer (LRET). Upon H2O2 addition, AgNPs can be etched and transformed into Ag(+), leading to inhibition of the LRET process and causing the recovery of upconversion luminescence. Based on the conversion of glucose into H2O2 by glucose oxidase, the DNA-AgNPs/UCNP nanocomposite can also be exploited for glucose sensing. Moreover, due to the non-autofluorescence offered by UCNPs, the approach developed can be applied to monitor glucose levels in human serum samples with satisfactory results.
authors.affiliation: State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China. xiachu@hnu.edu.cn.
authors.forename: Shuang
authors.initials: S
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China. xiachu@hnu.edu.cn.
authors.forename: Xiang-Juan
authors.initials: XJ
authors.lastname: Kong
authors.affiliation: State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China. xiachu@hnu.edu.cn.
authors.forename: Yao
authors.initials: Y
authors.lastname: Cen
authors.affiliation: State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China. xiachu@hnu.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China. xiachu@hnu.edu.cn.
authors.forename: Ru-Qin
authors.initials: RQ
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China. xiachu@hnu.edu.cn.
authors.forename: Xia
authors.initials: X
authors.lastname: Chu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D001786:Blood Glucose / Q000032:analysis*; D004247:DNA / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005947:Glucose / Q000032:analysis; D005949:Glucose Oxidase; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis*; D049449:Luminescence; D053768:Metal Nanoparticles / Q000737:chemistry; D053761:Nanocomposites / Q000737:chemistry*; D036103:Nanotechnology; D012834:Silver / Q000737:chemistry
chemical_list: D001786:Blood Glucose; D012834:Silver; D004247:DNA; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose