title: Influences of acidic reaction and hydrolytic conditions on monosaccharide composition analysis of acidic, neutral and basic polysaccharides.
abstract: Monosaccharide composition analysis is important for structural characterization of polysaccharides. To investigate the influences of acidic reaction and hydrolytic conditions on monosaccharide composition analysis of polysaccharides, we chose alginate, starch, chitosan and chondroitin sulfate as representative of acidic, neutral, basic and complex polysaccharides to compare the release degree of monosaccharides under different hydrolytic conditions. The hydrolysis stability of 10 monosaccharide standards was also explored. Results showed that the basic sugars were hard to release but stable, the acidic sugars (uronic acids) were easy to release but unstable, and the release and stability of neutral sugars were in between acidic and basic sugars. In addition, the hydrolysis process was applied to monosaccharide composition analysis of Hippocampus trimaculatus polysaccharide and the appropriate hydrolytic condition was accorded with that of the above four polysaccharides. Thus, different hydrolytic conditions should be used for the monosaccharide composition analysis of polysaccharides based on their structural characteristics.
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
authors.forename: Qing-Chi
authors.initials: QC
authors.lastname: Wang
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, Ocean University of China, Qingdao 266003, China. Electronic address: zhaoxia@ouc.edu.cn.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
authors.forename: Jiang-Hua
authors.initials: JH
authors.lastname: Pu
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Luan
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D002809:Chondroitin Sulfates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D000077553:Edaravone; D006868:Hydrolysis; D009005:Monosaccharides / Q000032:analysis*; D023701:Smegmamorpha; D013213:Starch / Q000737:chemistry*
chemical_list: D000464:Alginates; D009005:Monosaccharides; D013213:Starch; D002809:Chondroitin Sulfates; D048271:Chitosan; D000077553:Edaravone; D000983:Antipyrine
keywords: Alginate; Chitosan; Chondroitin sulfate; Hydrolytic condition; Monosaccharide composition; Starch