title: Preliminary Study on Gene Expression of Chitinase-Like Cytokines in Human Airway Epithelial Cell Under Chitin and Chitosan Microparticles Treatment.
abstract: Small-sized chitin and chitosan microparticles (MPs) reduce allergic inflammation. We examined the capacity of these glycans to stimulate A549 human airway epithelial cells to determine the feasibility of using of these glycans as allergic therapeutic modality. A549 cells were treated with MPs and then expressions levels of chitinase domain-containing 1 (CHID1) and chitinase 3-like 1 (CHI3L1) genes were determined by quantitative real-time PCR. IL-6 production was measured by ELISA. Chitin MPs resulted in upregulation of CHI3L1 expression by 35.7-fold while mRNA expression did not change with chitosan MPs. Compared to the untreated group, production of IL-6 was significantly decreased in the chitosan MPs-treated group, but chitin MPs treatment cause elevation of IL-6 level. This study demonstrates that chitin potently induces CHI3L1 expression, but chitosan is relatively inert. This effect and inhibition of pro-inflammatory cytokine (IL-6) suggest that chitosan MPs may possess more potential for therapeutic uses in human airway allergic inflammation.
authors.affiliation: Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Masumeh
authors.initials: M
authors.lastname: Alimohammadi
authors.affiliation: Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.|Department of Applied Cell Sciences, School of Advance Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Farshid
authors.initials: F
authors.lastname: Yeganeh
authors.affiliation: Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. m.mollahoseini@sbmu.ac.ir.|Phitochemistry Recearch Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. m.mollahoseini@sbmu.ac.ir.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Haji Molla Hoseini
mesh_terms: D000072283:A549 Cells; D001249:Asthma / Q000473:pathology; D002686:Chitin / Q000008:administration & dosage / Q000494:pharmacology*; D000071451:Chitinase-3-Like Protein 1 / Q000096:biosynthesis / Q000235:genetics; D002688:Chitinases; D048271:Chitosan / Q000008:administration & dosage / Q000494:pharmacology*; D016207:Cytokines / Q000096:biosynthesis* / Q000187:drug effects; D016503:Drug Delivery Systems; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D012137:Respiratory System / Q000473:pathology
chemical_list: D000071451:Chitinase-3-Like Protein 1; D016207:Cytokines; D015850:Interleukin-6; D002686:Chitin; D048271:Chitosan; D002688:Chitinases
keywords: allergic inflammation; chitin; chitinase-like proteins; chitosan