title: Ternary Hybrid γ-Fe2 O3 /Cr(VI) /Amine Oxidase Nanostructure for Electrochemical Sensing: Application for Polyamine Detection in Tumor Tissue.
abstract: Dichromate binds to surface-active maghemite nanoparticles (SAMNs) to form a stable core-shell nanostructures (SAMN@Cr(VI) ). The hybrid was characterized by Mössbauer spectroscopy, high-angle annular dark-field imaging, electron energy-loss spectroscopy, and electrochemical techniques, which revealed a strong interaction of dichromate with the nanoparticle surface. Electrochemical characterization showed lower charge-transfer resistance, better electrochemical performance, and more reversible electrochemical behavior with respect to naked SAMNs. Moreover, SAMN@Cr(VI) is an excellent electrocatalyst for hydrogen peroxide reduction. Furthermore, an enzyme, namely, bovine serum amine oxidase (BSAO: EC 1.4.3.6), was immobilized on SAMN@Cr(VI) by self-assembly to give a ternary hybrid nanostructured catalyst for polyamine oxidation (SAMN@Cr(VI) -BSAO). SAMN@Cr(VI) -BSAO was applied for the development of a reagentless, fast, inexpensive, and interference-free polyamine biosensor, which was successfully exploited for the discrimination of tumorous tissue from healthy tissue in human crude liver extracts.
authors.affiliation: Department of Comparative Biomedicine and Food Science, University of Padua, Agripolis-Viale dell'Università 16, Legnaro, 35020 (PD), Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Bonaiuto
authors.affiliation: Department of Comparative Biomedicine and Food Science, University of Padua, Agripolis-Viale dell'Università 16, Legnaro, 35020 (PD), Italy.|Regional Centre of Advanced Technologies and Materials, Palacky University, Olomouc, Czech Republic.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Magro
authors.affiliation: Department of Comparative Biomedicine and Food Science, University of Padua, Agripolis-Viale dell'Università 16, Legnaro, 35020 (PD), Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Baratella
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Palacky University, Olomouc, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Jakubec
authors.affiliation: Department of Comparative Biomedicine and Food Science, University of Padua, Agripolis-Viale dell'Università 16, Legnaro, 35020 (PD), Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Sconcerle
authors.affiliation: Department of Comparative Biomedicine and Food Science, University of Padua, Agripolis-Viale dell'Università 16, Legnaro, 35020 (PD), Italy.
authors.forename: Milo
authors.initials: M
authors.lastname: Terzo
authors.affiliation: Department of Molecular Medicine, University of Padua, Italy.|Proteomic Center of Padova University, VIMM and Padova University Hospital, Padua, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Miotto
authors.affiliation: Department of Biochemical Sciences "A. Rossi Fanelli", University of Rome "La Sapienza", Rome, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Macone
authors.affiliation: Department of Biochemical Sciences "A. Rossi Fanelli", University of Rome "La Sapienza", Rome, Italy.|CNR, Institute of Molecular Biology and Pathology, Rome, Italy.
authors.forename: Enzo
authors.initials: E
authors.lastname: Agostinelli
authors.affiliation: Unit of Hepatic Emergencies and Liver Transplantation, Department of Medicine, University of Padua, Padua, Italy.
authors.forename: Silvano
authors.initials: S
authors.lastname: Fasolato
authors.affiliation: Department of Molecular Medicine, University of Padua, Italy.
authors.forename: Rina
authors.initials: R
authors.lastname: Venerando
authors.affiliation: Department of Geosciences, University of Padua, Italy.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Salviulo
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Palacky University, Olomouc, Czech Republic.
authors.forename: Ondrej
authors.initials: O
authors.lastname: Malina
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Palacky University, Olomouc, Czech Republic.
authors.forename: Radek
authors.identifier: 0000-0002-3147-2196
authors.initials: R
authors.lastname: Zboril
authors.affiliation: Department of Comparative Biomedicine and Food Science, University of Padua, Agripolis-Viale dell'Università 16, Legnaro, 35020 (PD), Italy. fabio.vianello@unipd.it.|Regional Centre of Advanced Technologies and Materials, Palacky University, Olomouc, Czech Republic. fabio.vianello@unipd.it.
authors.forename: Fabio
authors.identifier: 0000-0002-4874-7205
authors.initials: F
authors.lastname: Vianello
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D006528:Carcinoma, Hepatocellular / Q000737:chemistry / Q000175:diagnosis; D002417:Cattle; D055664:Electrochemical Techniques; D004800:Enzymes, Immobilized; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D008113:Liver Neoplasms / Q000737:chemistry / Q000175:diagnosis*; D060328:Magnetic Phenomena; D050997:Nanomedicine; D053758:Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000737:chemistry / Q000175:diagnosis*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry; D010316:Particle Size; D011073:Polyamines / Q000032:analysis*; D013499:Surface Properties
chemical_list: D004800:Enzymes, Immobilized; D005290:Ferric Compounds; D011073:Polyamines; C000499:ferric oxide; D010088:Oxidoreductases
keywords: biosensors; cancer; chromium; enzymes; nanostructures