title: Dietary Lipid Type, Rather Than Total Number of Calories, Alters Outcomes of Enteric Infection in Mice.
abstract: Dietary lipids modulate immunity, yet the means by which specific fatty acids affect infectious disease susceptibility remains unclear. Deciphering lipid-induced immunity is critical to understanding the balance required for protecting against pathogens while avoiding chronic inflammatory diseases. To understand how specific lipids alter susceptibility to enteric infection, we fed mice isocaloric, high-fat diets composed of corn oil (rich in n-6 polyunsaturated fatty acids [n-6 PUFAs]), olive oil (rich in monounsaturated fatty acids), or milk fat (rich in saturated fatty acids) with or without fish oil (rich in n-3 PUFAs). After 5 weeks of dietary intervention, mice were challenged with Citrobacter rodentium, and pathological responses were assessed. Olive oil diets resulted in little colonic pathology associated with intestinal alkaline phosphatase, a mucosal defense factor that detoxifies lipopolysaccharide. In contrast, while both corn oil and milk fat diets resulted in inflammation-induced colonic damage, only milk fat induced compensatory protective responses, including short chain fatty acid production. Fish oil combined with milk fat, unlike unsaturated lipid diets, had a protective effect associated with intestinal alkaline phosphatase activity. Overall, these results reveal that dietary lipid type, independent of the total number of calories associated with the dietary lipid, influences the susceptibility to enteric damage and the benefits of fish oil during infection.
authors.affiliation: Department of Biology, University of British Columbia, Kelowna, Canada.
authors.forename: Daniella
authors.initials: D
authors.lastname: DeCoffe
authors.affiliation: Department of Biology, University of British Columbia, Kelowna, Canada.
authors.forename: Candice
authors.initials: C
authors.lastname: Quin
authors.affiliation: Department of Biology, University of British Columbia, Kelowna, Canada.
authors.forename: Sandeep K
authors.initials: SK
authors.lastname: Gill
authors.affiliation: Department of Biology, University of British Columbia, Kelowna, Canada.
authors.forename: Nishat
authors.initials: N
authors.lastname: Tasnim
authors.affiliation: Department of Biology, University of British Columbia, Kelowna, Canada.
authors.forename: Kirsty
authors.initials: K
authors.lastname: Brown
authors.affiliation: Department of Biology, University of British Columbia, Kelowna, Canada.
authors.forename: Artem
authors.initials: A
authors.lastname: Godovannyi
authors.affiliation: Department of Biology, University of British Columbia, Kelowna, Canada.
authors.forename: Chuanbin
authors.initials: C
authors.lastname: Dai
authors.affiliation: Department of Biology, University of British Columbia, Kelowna, Canada.
authors.forename: Nijiati
authors.initials: N
authors.lastname: Abulizi
authors.affiliation: Department of Biology, University of British Columbia, Kelowna, Canada.
authors.forename: Yee Kwan
authors.initials: YK
authors.lastname: Chan
authors.affiliation: Department of Biology, University of British Columbia, Kelowna, Canada.
authors.forename: Sanjoy
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Department of Biology, University of British Columbia, Kelowna, Canada.
authors.forename: Deanna L
authors.initials: DL
authors.lastname: Gibson
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D044082:Citrobacter rodentium; D003106:Colon / Q000382:microbiology; D003314:Corn Oil / Q000008:administration & dosage / Q000627:therapeutic use; D059305:Diet, High-Fat; D004041:Dietary Fats / Q000276:immunology / Q000627:therapeutic use*; D004198:Disease Susceptibility; D002149:Energy Intake; D004756:Enterobacteriaceae Infections / Q000178:diet therapy* / Q000276:immunology / Q000517:prevention & control; D005260:Female; D005395:Fish Oils / Q000627:therapeutic use; D006801:Humans; D008070:Lipopolysaccharides / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008892:Milk; D000069463:Olive Oil / Q000008:administration & dosage / Q000627:therapeutic use; D010766:Phosphorylation; D016896:Treatment Outcome
chemical_list: D004041:Dietary Fats; D005395:Fish Oils; D008070:Lipopolysaccharides; D000069463:Olive Oil; D003314:Corn Oil
keywords: Citrobacter rodentium infection; colitis; corn oil; fish oil; high-fat diets; inflammation; intestinal alkaline phosphatase; milk fat; nutrition; olive oil