title: Quantitative Proteomic Analysis of Venoms from Russian Vipers of Pelias Group: Phospholipases A₂ are the Main Venom Components.
abstract: Venoms of most Russian viper species are poorly characterized. Here, by quantitative chromato-mass-spectrometry, we analyzed protein and peptide compositions of venoms from four Vipera species (V. kaznakovi, V. renardi, V. orlovi and V. nikolskii) inhabiting different regions of Russia. In all these species, the main components were phospholipases A₂, their content ranging from 24% in V. orlovi to 65% in V. nikolskii. Altogether, enzyme content in venom of V. nikolskii reached ~85%. Among the non-enzymatic proteins, the most abundant were disintegrins (14%) in the V. renardi venom, C-type lectin like (12.5%) in V. kaznakovi, cysteine-rich venom proteins (12%) in V. orlovi and venom endothelial growth factors (8%) in V. nikolskii. In total, 210 proteins and 512 endogenous peptides were identified in the four viper venoms. They represented 14 snake venom protein families, most of which were found in the venoms of Vipera snakes previously. However, phospholipase B and nucleotide degrading enzymes were reported here for the first time. Compositions of V. kaznakovi and V. orlovi venoms were described for the first time and showed the greatest similarity among the four venoms studied, which probably reflected close relationship between these species within the "kaznakovi" complex.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia. xerx222@gmail.com.
authors.forename: Sergey I
authors.initials: SI
authors.lastname: Kovalchuk
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia. rustam.ziganshin@gmail.com.
authors.forename: Rustam H
authors.initials: RH
authors.lastname: Ziganshin
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia. vladislavstarkov@mail.ru.
authors.forename: Vladislav G
authors.initials: VG
authors.lastname: Starkov
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia. vits@mx.ibch.ru.
authors.forename: Victor I
authors.initials: VI
authors.lastname: Tsetlin
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia. utkin@mx.ibch.ru.
authors.forename: Yuri N
authors.initials: YN
authors.lastname: Utkin
mesh_terms: D000818:Animals; D054467:Phospholipases A2 / Q000032:analysis*; D040901:Proteomics; D030162:Reptilian Proteins / Q000032:analysis*; D014757:Viper Venoms / Q000737:chemistry*; D017819:Viperidae
chemical_list: D030162:Reptilian Proteins; D014757:Viper Venoms; D054467:Phospholipases A2
keywords: Vipera kaznakovi; Vipera nikolskii; Vipera orlovi; Vipera renardi; mass-spectrometry; proteome; snake venom; viper