title: Evaluation of canine red blood cell quality after processing with an automated cell salvage device.
abstract: OBJECTIVE
  To evaluate the properties of RBC concentrate harvested after processing fresh whole blood units from healthy dogs with an automated cell salvage device.
  
  
  METHODS
  Prospective, in vitro, experimental study.
  
  
  METHODS
  University teaching hospital.
  
  
  METHODS
  Sixteen healthy, privately owned dogs of various breeds.
  
  
  METHODS
  Fresh canine whole blood collected in bags with citrate phosphate dextrose adenine solution was processed with an automated cell salvage device and analyzed in vitro. Laboratory values determined before (baseline, from a catheter sample) and after processing RBCs (procRBCs) included a complete blood count, selected blood chemistry analytes, erythrocyte osmotic resistance, whole blood viscosity, RBC aggregation, and RBC deformability.
  
  
  RESULTS
  Total recovery of RBCs was 80% ± 12%. Hematocrit of the procRBCs yielded by the device was 77% ± 3.7% (mean ± standard deviation). Gross morphology of the RBCs remained unchanged. The mean corpuscular volume, erythrocyte osmotic resistance, RBC deformability, RBC aggregation, and the activity of lactate dehydrogenase showed minor but statistically significant changes from baseline. No differences in the concentrations of free hemoglobin were observed. Whole blood viscosity was less in the procRBCs. Seventy-seven percent (mean) of the platelets were washed out, while a mean of 57% of the leukocytes remained in the procRBCs.
  
  
  CONCLUSIONS
  Although processing canine blood with this automated cell salvage device leads to slight changes in some properties of RBCs, most of these changes are comparable to changes seen in human blood after processing. Present data indicate that the use of this cell salvage device does not induce changes in canine RBC concentrate that would preclude its use for transfusion.
authors.affiliation: Department of Anesthesiology and Perioperative Intensive Care, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Nina
authors.initials: N
authors.lastname: Hofbauer
authors.affiliation: Decentralized Biomedical Facilities of the Medical University of Vienna, Vienna, Austria.
authors.forename: Ursula
authors.initials: U
authors.lastname: Windberger
authors.affiliation: Department of Pathobiology, Central Laboratory, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Ilse
authors.initials: I
authors.lastname: Schwendenwein
authors.affiliation: Department of Biomedical Sciences, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Alexander
authors.initials: A
authors.lastname: Tichy
authors.affiliation: Department of Anesthesiology and Perioperative Intensive Care, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Eva
authors.initials: E
authors.lastname: Eberspächer
mesh_terms: D000818:Animals; D001331:Automation; D001781:Blood Component Removal / Q000295:instrumentation* / Q000662:veterinary; D001793:Blood Preservation / Q000662:veterinary*; D004283:Dog Diseases / Q000628:therapy*; D004285:Dogs; D017707:Erythrocyte Transfusion / Q000662:veterinary*; D004912:Erythrocytes / Q000166:cytology*; D011446:Prospective Studies
keywords: autologous transfusion; autotransfusion; dog; erythrocytes; hemoabdomen; packed RBC