title: Correlation of IL-18 with Tryptase in Atopic Asthma and Induction of Mast Cell Accumulation by IL-18.
abstract: Interleukin- (IL-) 18 and tryptase were previously reported to relate to asthma, but the correlation between these two potent proinflammatory molecules in asthma and their roles in mast cell accumulation remain uninvestigated. Using flow cytometric analysis technique and ovalbumin- (OVA-) sensitized mouse model, it was found that IL-18 and tryptase levels in the plasma of moderate and severe asthma were elevated, and they correlated well with each other. Tryptase and agonist peptides of protease activated receptor- (PAR-) 2 induced substantial quantity of IL-18 release. IL-18 and tryptase provoked mast cell accumulation in peritoneum of OVA-sensitized mice. OVA-sensitization increased number of IL-18 receptor (R)(+) mast cells. IL-18 and tryptase induced dramatic increase in IL-18R(+) mast cells and mean fluorescence intensity (MFI) of IL-18R on mast cells. Moreover, while IL-18 induced an increase in PAR-2(+) mast cells in nonsensitized mice, IL-18 and tryptase provoked increases in IL-4 and thymic stromal lymphopoietin (TSLP) in the peritoneum of OVA-sensitized mice. In summary, the correlation between IL-18 and tryptase in plasma of patients with asthma indicates close interactions between them, which should be considered for development of anti-IL-18 and antitryptase therapies. Interactions between IL-18 and tryptase may contribute to mast cell recruitment in asthma.
authors.affiliation: Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, China.
authors.forename: Junling
authors.initials: J
authors.lastname: Wang
authors.affiliation: Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, China.
authors.forename: Huiyun
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, China.
authors.forename: Wenjiao
authors.initials: W
authors.lastname: Zheng
authors.affiliation: The PLA Center of Respiratory and Allergic Disease Diagnosing Management, General Hospital of Shenyang Military Area Command, Shenyang 110840, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Xie
authors.affiliation: Clinical Research Centre, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.
authors.forename: Hongling
authors.initials: H
authors.lastname: Yan
authors.affiliation: The PLA Center of Respiratory and Allergic Disease Diagnosing Management, General Hospital of Shenyang Military Area Command, Shenyang 110840, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Lin
authors.affiliation: Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, China.
authors.forename: Shaoheng
authors.initials: S
authors.lastname: He
mesh_terms: D001249:Asthma / Q000097:blood* / Q000201:enzymology*; D016207:Cytokines / Q000378:metabolism; D020382:Interleukin-18 / Q000097:blood*; D015847:Interleukin-4 / Q000378:metabolism; D008407:Mast Cells / Q000378:metabolism*; D044464:Receptor, PAR-2 / Q000819:agonists / Q000378:metabolism; D053724:Receptors, Interleukin-18 / Q000378:metabolism; D053802:Tryptases / Q000097:blood*; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D016207:Cytokines; D020382:Interleukin-18; D044464:Receptor, PAR-2; D053724:Receptors, Interleukin-18; D015847:Interleukin-4; D053802:Tryptases; D000094632:Thymic Stromal Lymphopoietin