title: Overexpression of hyaluronan synthase 2 and gonadotropin receptors in cumulus cells of goats subjected to one-shot eCG/FSH hormonal treatment for ovarian stimulation.
abstract: Hormonal ovarian stimulation may affect transcripts in somatic cells of cumulus-oocyte complexes (COCs) and affect the resulting oocyte quality. Here, in parallel with morphological classification and in vitro maturation (IVM) rate analysis, we investigated the expression of hyaluronan synthase 2 (HAS2), gonadotropic receptors (FSHR and LHR) and connexin 43 (GJA1) in cumulus cells (CCs) from goat COCs after multi-dose FSH (MD) or one-shot FSH/eCG (OS) treatments, using bovine COCs as control groups. The MD treatment produced more large follicles, and the resulting COCs had a better morphology and IVM rate than were obtained with OS. The OS treatment produced COCs with increased HAS2, FSHR, LHR and GJA1 expression. This gene expression pattern was also observed in the CCs of COCs that showed poor morphological characteristics. On the other hand, the mRNA levels were more similar between groups after IVM; FSHR and LHR were the main genes that showed decreased expression. Some events that occurred in bovine CCs during IVM, such as cell expansion, increased HAS2 expression and decreased GJA1 expression, were less evident or did not occur in goat COCs. In conclusion, increasing HAS2, FSHR, LHR and GJA1 expression in goat COCs does not confer greater meiotic competence to oocytes. Instead, it may result from poor regulation of gene expression in CCs by lower quality oocytes. Finally, cumulus expansion, together with HAS2 upregulation and GJA1 downregulation, seems to be more important for bovine COCs than for goat COCs. Additional studies are needed to investigate the importance of other HAS isoforms and connexins in goat COCs.
authors.affiliation: Laboratory of Physiology and Control of Reproduction, Veterinary School, State University of Ceará, Av. Doutor Silas Munguba, 1700, Campus do Itaperi, 60.714-903 Fortaleza-CE, Brazil.
authors.forename: Juliana D R
authors.initials: JD
authors.lastname: Santos
authors.affiliation: Laboratory of Physiology and Control of Reproduction, Veterinary School, State University of Ceará, Av. Doutor Silas Munguba, 1700, Campus do Itaperi, 60.714-903 Fortaleza-CE, Brazil.
authors.forename: Ribrio I T P
authors.initials: RI
authors.lastname: Batista
authors.affiliation: Laboratory of Physiology and Control of Reproduction, Veterinary School, State University of Ceará, Av. Doutor Silas Munguba, 1700, Campus do Itaperi, 60.714-903 Fortaleza-CE, Brazil.
authors.forename: Livia C
authors.initials: LC
authors.lastname: Magalhães
authors.affiliation: Laboratory of Physiology and Control of Reproduction, Veterinary School, State University of Ceará, Av. Doutor Silas Munguba, 1700, Campus do Itaperi, 60.714-903 Fortaleza-CE, Brazil.
authors.forename: Alexandre R
authors.initials: AR
authors.lastname: Paula
authors.affiliation: Laboratory of Physiology and Control of Reproduction, Veterinary School, State University of Ceará, Av. Doutor Silas Munguba, 1700, Campus do Itaperi, 60.714-903 Fortaleza-CE, Brazil.
authors.forename: Samara S
authors.initials: SS
authors.lastname: Souza
authors.affiliation: Laboratory of Animal Biotechnology. School of Agronomy. Buenos Aires University, Av. San Martín 4453, C1417 Buenos Aires, Argentina.
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Salamone
authors.affiliation: Laboratory of Physiology and Control of Reproduction, Veterinary School, State University of Ceará, Av. Doutor Silas Munguba, 1700, Campus do Itaperi, 60.714-903 Fortaleza-CE, Brazil.
authors.forename: Maajid H
authors.initials: MH
authors.lastname: Bhat
authors.affiliation: Laboratory of Physiology and Control of Reproduction, Veterinary School, State University of Ceará, Av. Doutor Silas Munguba, 1700, Campus do Itaperi, 60.714-903 Fortaleza-CE, Brazil.
authors.forename: Dárcio I A
authors.initials: DI
authors.lastname: Teixeira
authors.affiliation: Laboratory of Physiology and Control of Reproduction, Veterinary School, State University of Ceará, Av. Doutor Silas Munguba, 1700, Campus do Itaperi, 60.714-903 Fortaleza-CE, Brazil.
authors.forename: Vicente J F
authors.initials: VJ
authors.lastname: Freitas
authors.affiliation: Laboratory of Physiology and Control of Reproduction, Veterinary School, State University of Ceará, Av. Doutor Silas Munguba, 1700, Campus do Itaperi, 60.714-903 Fortaleza-CE, Brazil. Electronic address: lucianamelo.uece@gmail.com.
authors.forename: Luciana M
authors.initials: LM
authors.lastname: Melo
mesh_terms: D000818:Animals; D006063:Chorionic Gonadotropin / Q000008:administration & dosage / Q000494:pharmacology*; D054885:Cumulus Cells / Q000201:enzymology* / Q000378:metabolism; D004338:Drug Combinations; D005260:Female; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D014453:Glucuronosyltransferase / Q000378:metabolism*; D006728:Hormones / Q000008:administration & dosage / Q000494:pharmacology; D010062:Ovulation Induction / Q000662:veterinary*; D012102:Reproductive Control Agents / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D006063:Chorionic Gonadotropin; D004338:Drug Combinations; D006728:Hormones; D012102:Reproductive Control Agents; D005640:Follicle Stimulating Hormone; D014453:Glucuronosyltransferase
keywords: Connexin 43; Cumulus cell; FSHR; HAS2; LHR; Oocyte maturation