title: Analysis of Th Cell-related Cytokine Production in Behçet Disease Patients with Uveitis Before and After Infliximab Treatment.
abstract: OBJECTIVE
  To examine antigen-stimulated cytokine production by Behçet disease patients (BD) before and after infliximab infusion.
  
  
  METHODS
  PBMCs were obtained before and after infliximab infusion in BD patients with or without recurrent uveitis during at least 1 year of infliximab therapy, and from healthy subjects. PBMCs were cultured with IRBP, and Th-related cytokines in cultures were measured.
  
  
  RESULTS
  Levels of IL-4, IL-6, IL-10 IL-17A, IL-17F, IL-31, IFN-γ, and TNFα were higher in BD before infliximab infusion than in healthy subjects, and these levels were the highest in BD with recurrent uveitis. After infliximab infusion, these cytokine levels were reduced to a greater extent in BD without recurrent uveitis than in BD with recurrence.
  
  
  CONCLUSIONS
  Th-related cytokines produced by IRBP-stimulated PBMCs were elevated in BD, and infliximab infusion suppressed these cytokines to a greater extent in BD without recurrent uveitis than in those with recurrence.
authors.affiliation: a Department of Ophthalmology , National Defense Medical College , Saitama , Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Takeuchi
authors.affiliation: a Department of Ophthalmology , National Defense Medical College , Saitama , Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Karasawa
authors.affiliation: a Department of Ophthalmology , National Defense Medical College , Saitama , Japan.
authors.forename: Kohzou
authors.initials: K
authors.lastname: Harimoto
authors.affiliation: a Department of Ophthalmology , National Defense Medical College , Saitama , Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Tanaka
authors.affiliation: a Department of Ophthalmology , National Defense Medical College , Saitama , Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Shibata
authors.affiliation: a Department of Ophthalmology , National Defense Medical College , Saitama , Japan.
authors.forename: Tomohito
authors.initials: T
authors.lastname: Sato
authors.affiliation: c Laboratory of Immunology, National Eye Institute, National Institutes of Health , Bethesda , Maryland , USA.
authors.forename: Rachel R
authors.initials: RR
authors.lastname: Caspi
authors.affiliation: b Department of Developmental Anatomy and Regenerative Biology , National Defense Medical College , Saitama , Japan.
authors.forename: Masataka
authors.initials: M
authors.lastname: Ito
mesh_terms: D000328:Adult; D001528:Behcet Syndrome / Q000188:drug therapy* / Q000276:immunology; D016207:Cytokines / Q000378:metabolism*; D005136:Eye Proteins / Q000494:pharmacology; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D000069285:Infliximab / Q000627:therapeutic use*; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D012177:Retinol-Binding Proteins / Q000494:pharmacology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D014605:Uveitis / Q000188:drug therapy* / Q000276:immunology; D000099214:Retinol-Binding Proteins, Interstitial
chemical_list: D016207:Cytokines; D005136:Eye Proteins; D007166:Immunosuppressive Agents; D000069285:Infliximab; D012177:Retinol-Binding Proteins; D000099214:Retinol-Binding Proteins, Interstitial
keywords: Behçet disease; infliximab; uveitis