title: Resveratrol ameliorates 2,4-dinitrofluorobenzene-induced atopic dermatitis-like lesions through effects on the epithelium.
abstract: Background. Resveratrol is a natural polyphenol that exhibits anti-inflammatory effects. The aim of this study was to investigate the effects of resveratrol treatment on epithelium-derived cytokines and epithelial apoptosis in a murine model of atopic dermatitis-like lesions. Material and Methods. Atopic dermatitis-like lesions were induced in BALB/c mice by repeated application of 2,4-dinitrofluorobenzene to shaved dorsal skin. Twenty-one BALB/c mice were divided into three groups: group I (control), group II (vehicle control), and group III (resveratrol). Systemic resveratrol (30 mg/kg/day) was administered repeatedly during the 6th week of the experiment. After the mice had been sacrificed, skin tissues were examined histologically for epithelial thickness. Epithelial apoptosis (caspase-3) and epithelium-derived cytokines [interleukin (IL)-25, IL-33, and thymic stromal lymphopoietin (TSLP)] were evaluated immunohistochemically. Results. Epithelial thickness and the numbers of IL-25, IL-33, TSLP and caspase-3-positive cells were significantly higher in group II compared to group I mice. There was significant improvement in epithelial thickness in group III compared with group II mice (p < 0.05). The numbers of IL-25, IL-33, and TSLP-positive cells in the epithelium were lower in group III than in group II mice (p < 0.05). The number of caspase-3-positive cells, as an indicator of apoptosis, in the epithelium was significantly lower in group III than in group II mice (p < 0.05). Conclusion. Treatment with resveratrol was effective at ameliorating histological changes and inflammation by acting on epithelium-derived cytokines and epithelial apoptosis.
authors.affiliation: Faculty of Medicine, Department of Pediatric Allergy and Immunology, Dokuz Eylül University , Izmir , Turkey.
authors.forename: Sule
authors.initials: S
authors.lastname: Caglayan Sozmen
authors.affiliation: Faculty of Medicine, Department of Microbiology, Dokuz Eylül University , Izmir , Turkey.
authors.forename: Meral
authors.initials: M
authors.lastname: Karaman
authors.affiliation: Faculty of Medicine, Department of Histology and Embryology, Dokuz Eylül University , Izmir , Turkey.
authors.forename: Serap
authors.initials: S
authors.lastname: Cilaker Micili
authors.affiliation: Faculty of Medicine, Department of Pediatric Allergy and Immunology, Dokuz Eylül University , Izmir , Turkey.
authors.forename: Sakine
authors.initials: S
authors.lastname: Isik
authors.affiliation: Faculty of Medicine, Department of Pediatric Allergy and Immunology, Dokuz Eylül University , Izmir , Turkey.
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Arikan Ayyildiz
authors.affiliation: Faculty of Medicine, Department of Histology and Embryology, Dokuz Eylül University , Izmir , Turkey.
authors.forename: Alper
authors.initials: A
authors.lastname: Bagriyanik
authors.affiliation: Faculty of Medicine, Department of Pediatric Allergy and Immunology, Dokuz Eylül University , Izmir , Turkey.
authors.forename: Nevin
authors.initials: N
authors.lastname: Uzuner
authors.affiliation: Faculty of Medicine, Department of Pediatric Allergy and Immunology, Dokuz Eylül University , Izmir , Turkey.
authors.forename: Ozkan
authors.initials: O
authors.lastname: Karaman
keywords: Atopic dermatitis; Mice; Resveratrol; Treatment