title: Whole-transcriptome analyses of the Sapsaree, a Korean natural monument, before and after exercise-induced stress.
abstract: BACKGROUND
  The Sapsaree (Canis familiaris) is a Korean native dog that is very friendly, protective, and loyal to its owner, and is registered as a natural monument in Korea (number: 368). To investigate large-scale gene expression profiles and identify the genes related to exercise-induced stress in the Sapsaree, we performed whole-transcriptome RNA sequencing and analyzed gene expression patterns before and after exercise performance.
  
  
  RESULTS
  We identified 525 differentially expressed genes in ten dogs before and after exercise. Gene Ontology classification and KEGG pathway analysis revealed that the genes were mainly involved in metabolic processes, such as programmed cell death, protein metabolic process, phosphatidylinositol signaling system, and cation binding in cytoplasm. The ten Sapsarees could be divided into two groups based on the gene expression patterns before and after exercise. The two groups were significantly different in terms of their basic body type (p ≤ 0.05). Seven representative genes with significantly different expression patterns before and after exercise between the two groups were chosen and characterized.
  
  
  CONCLUSIONS
  Body type had a significant effect on the patterns of differential gene expression induced by exercise. Whole-transcriptome sequencing is a useful method for investigating the biological characteristics of the Sapsaree and the large-scale genomic differences of canines in general.
authors.affiliation: SEEDERS Inc, Daejeon, 34015 Republic of Korea.
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Kim
authors.affiliation: SEEDERS Inc, Daejeon, 34015 Republic of Korea.
authors.forename: Junkyung
authors.initials: J
authors.lastname: Choe
authors.affiliation: SEEDERS Inc, Daejeon, 34015 Republic of Korea.
authors.forename: Jeong Hee
authors.initials: JH
authors.lastname: Lee
authors.affiliation: SEEDERS Inc, Daejeon, 34015 Republic of Korea.
authors.forename: Woong Bom
authors.initials: WB
authors.lastname: Kim
authors.affiliation: SEEDERS Inc, Daejeon, 34015 Republic of Korea.
authors.forename: Whan
authors.initials: W
authors.lastname: Cho
authors.affiliation: The Korean Sapsaree Foundation, Gyeongsan, Gyeongbuk 38412 Republic of Korea.
authors.forename: Ji Hong
authors.initials: JH
authors.lastname: Ha
authors.affiliation: The Korean Sapsaree Foundation, Gyeongsan, Gyeongbuk 38412 Republic of Korea.
authors.forename: Ki Jin
authors.initials: KJ
authors.lastname: Kwon
authors.affiliation: Department of Animal training and event, Daekyeung University, Gyeongsan, Gyeongbuk 38547 Republic of Korea.
authors.forename: Kook Il
authors.initials: KI
authors.lastname: Han
authors.affiliation: SEEDERS Inc, Daejeon, 34015 Republic of Korea.
authors.forename: Sung-Hwan
authors.initials: SH
authors.lastname: Jo
keywords: Bioinformatics; Exercise; NGS; Physical stress; RNA-Seq; Sapsaree; Transcriptome