title: Fluorescence "turn-on" determination of H2O2 using multilayer porous SiO2/NGQDs and PdAu mimetics enzymatic/oxidative cleavage of single-stranded DNA.
abstract: A 3D microfluidic paper-based fluorescence analytical device with hollow channels based on the turn-on switching of a resonance energy transfer triggered by the •OH induced cleavage of a DNA strand was successfully constructed. And this fluorescent nanoplatform was first designed to achieve in situ and real-time determination of H2O2 released from cancer cells to obtain an accurate determination. With optimal conditions, the proposed method displayed excellent analytical performance for the detection of H2O2 ranging from 0.3 to 1.0mM with a detection limit of 0.1nM. The favorable performances of this sensor were due to the peroxidase-like activity of nitrogen-doped graphene quantum dots (multilayer porous SiO2 act as stabilizer to load more nitrogen-doped graphene quantum dots for signal amplification) and folic acid-pPdAu/GO (which also could act as an efficient fluorescence quencher and a recognition element of cancer cells by folic acid). It was worth noting that it could be used for visually determined the flux of H2O2 from the cells. Therefore, the developed biosensor holds potential for ultrasensitive quantitative analysis of H2O2 and supplies valuable information for diabetes mellitus research and clinical diagnosis.
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Liang
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Lan
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Su
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China; Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, University of Jinan, Jinan 250022, PR China. Electronic address: chm_gesg@163.com.
authors.forename: Shenguang
authors.initials: S
authors.lastname: Ge
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Haiyun
authors.initials: H
authors.lastname: Liu
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yan
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation; D004277:DNA, Single-Stranded / Q000737:chemistry*; D004867:Equipment Design; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation*; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis*; D056656:Lab-On-A-Chip Devices; D057230:Limit of Detection; D061986:MCF-7 Cells; D009584:Nitrogen / Q000737:chemistry; D010165:Palladium / Q000737:chemistry; D009195:Peroxidase / Q000737:chemistry; D016062:Porosity; D045663:Quantum Dots / Q000737:chemistry* / Q000648:ultrastructure; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D004277:DNA, Single-Stranded; D010165:Palladium; D006046:Gold; D012822:Silicon Dioxide; D006108:Graphite; D006861:Hydrogen Peroxide; D009195:Peroxidase; D009584:Nitrogen
keywords: Fluorescence; Hollow channels; Nitrogen-doped graphene quantum dots; PdAu mimetics enzymatic; Visualization