title: [TALEN-mediated MYH9 Knock-down and its influence on cell cycle and apoptosis of MGC803 cell line].
abstract: OBJECTIVE
  To construct a MYH9 gene knockout model in MGC803 cell line using transcription activator-like effector nuclease (TALEN) and observe its effect on cell cycle and apoptosis.
  
  
  METHODS
  According to FastTALE(TM) TALEN Kit, we designed TALEN pairs and constructed the plasmids targeting to MYH9 gene. After detecting their activity in MGC803 cells by plasmid transfection, DNA sequencing, RT-PCR and western blot, we selected the monoclonal cells and studied the changes in the cell cycle and apoptosis.
  
  
  RESULTS
  MYH9 gene could not be knocked out but knocked down in selected MGC803 monoclonal cells, which caused cell cycle arrested at G2/M phase (P<0.05) and a significant increase in the cell number with early apoptosis (P<0.01).
  
  
  CONCLUSIONS
  We successfully generated a MYH9 knockdown model in MGC803 cell lines by TALEN, which could be in favor of MYH9 function study in gastric cancer.
authors.affiliation: Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.E-mail: zhuxianjun1989@163.com.
authors.forename: Xian-Jun
authors.initials: XJ
authors.lastname: Zhu
authors.forename: Hai-Jun
authors.initials: HJ
authors.lastname: Deng
authors.forename: Geng-Tai
authors.initials: GT
authors.lastname: Ye
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Shen
authors.forename: Feng-Ping
authors.initials: FP
authors.lastname: Li
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: Guo
authors.forename: Qing-Bin
authors.initials: QB
authors.lastname: Yang
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.forename: Guo-Xin
authors.initials: GX
authors.lastname: Li
mesh_terms: D017209:Apoptosis; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D055785:Gene Knockdown Techniques; D006801:Humans; D020409:Molecular Motor Proteins / Q000235:genetics*; D018995:Myosin Heavy Chains / Q000235:genetics*; D010957:Plasmids; D013274:Stomach Neoplasms; D014162:Transfection
chemical_list: C103814:MYH9 protein, human; D020409:Molecular Motor Proteins; D018995:Myosin Heavy Chains