title: Mixed thread/paper-based microfluidic chips as a platform for glucose assays.
abstract: A novel microfluidic thread/paper-based analytical device (μTPAD) to detect glucose through a colorimetric assay is described. The μTPAD was fabricated from nylon thread trifurcated into three channels terminating at analysis sites comprised of circular zones of chromatography paper, which have previously been spotted with glucose of different concentrations. A solution of glucose oxidase (GOx), horseradish peroxidase (HRP), and potassium iodide (KI) is transported via capillary action to the analysis sites where a yellow-brown color is observed indicating oxidation of iodide to iodine. The device was then dried, scanned, and analyzed yielding a correlation between yellow intensity and glucose concentrations. Both a flat platform constructed mainly of tape, and a cone platform constructed from tape and polyvinyl chloride, are described. Studies to quantitate glucose in artificial urine showed good correlation using the μTPAD.
authors.affiliation: Department of Chemistry and Biochemistry, California State University, Los Angeles, CA, USA.
authors.forename: Ariana
authors.initials: A
authors.lastname: Gonzalez
authors.affiliation: Department of Chemistry and Biochemistry, California State University, Los Angeles, CA, USA.
authors.forename: Lissette
authors.initials: L
authors.lastname: Estala
authors.affiliation: Department of Chemistry and Biochemistry, California State University, Los Angeles, CA, USA.
authors.forename: Michelle
authors.initials: M
authors.lastname: Gaines
authors.affiliation: Department of Chemistry and Biochemistry, California State University, Los Angeles, CA, USA.
authors.forename: Frank A
authors.initials: FA
authors.lastname: Gomez
mesh_terms: D003116:Color; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D011193:Potassium Iodide
chemical_list: D011193:Potassium Iodide; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D005947:Glucose
keywords: Glucose oxidase; Point-of-care diagnostic device; Thread/paper microfluidics