title: Evaluation of expansile nanoparticle tumor localization and efficacy in a cancer stem cell-derived model of pancreatic peritoneal carcinomatosis.
abstract: OBJECTIVE
  To evaluate the tumor localization and efficacy pH-responsive expansile nanoparticles (eNPs) as a drug delivery system for pancreatic peritoneal carcinomatosis (PPC) modeled in nude rats.
  
  
  METHODS
  A Panc-1-cancer stem cell xeno1graft model of PPC was validated in vitro and in vivo. Tumor localization was tracked via in situ imaging of fluorescent eNPs. Survival of animals treated with paclitaxel-loaded eNPs (PTX-eNPs) was evaluated in vivo.
  
  
  RESULTS
  The Panc-1-cancer stem cell xenograft model recapitulates significant features of PPC. Rhodamine-labeled eNPs demonstrate tumor-specific, dose- and time-dependent localization to macro- and microscopic tumors following intraperitoneal injection. PTX-eNPs are as effective as free PTX in treating established PPC; but, PTX-eNPs result in fewer side effects.
  
  
  CONCLUSIONS
  eNPs are a promising tool for the detection and treatment of PPC.
authors.affiliation: Department of Medicine & Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA 02118, USA.
authors.forename: Victoria Lm
authors.initials: VL
authors.lastname: Herrera
authors.affiliation: Departments of Biomedical Engineering & Chemistry, Boston University, Boston, MA 02215, USA.
authors.forename: Aaron H
authors.initials: AH
authors.lastname: Colby
authors.affiliation: Department of Medicine & Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA 02118, USA.
authors.forename: Glaiza Al
authors.initials: GA
authors.lastname: Tan
authors.affiliation: Department of Medicine & Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA 02118, USA.
authors.forename: Ann M
authors.initials: AM
authors.lastname: Moran
authors.affiliation: Department of Pathology & Laboratory Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: O'Brien
authors.affiliation: Division of Thoracic Surgery, Department of Surgery, Brigham & Women's Hospital, Boston, MA 02115, USA.
authors.forename: Yolonda L
authors.initials: YL
authors.lastname: Colson
authors.affiliation: Department of Medicine & Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA 02118, USA.
authors.forename: Nelson
authors.initials: N
authors.lastname: Ruiz-Opazo
authors.affiliation: Department of Medicine & Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA 02118, USA.|Departments of Biomedical Engineering & Chemistry, Boston University, Boston, MA 02215, USA.
authors.forename: Mark W
authors.initials: MW
authors.lastname: Grinstaff
mesh_terms: D000818:Animals; D002277:Carcinoma / Q000188:drug therapy* / Q000473:pathology; D004195:Disease Models, Animal; D016503:Drug Delivery Systems; D006801:Humans; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000473:pathology; D017239:Paclitaxel / Q000008:administration & dosage / Q000737:chemistry; D010190:Pancreatic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D010537:Peritoneum / Q000187:drug effects / Q000473:pathology; D051381:Rats; D012235:Rhodamines / Q000737:chemistry; D023041:Xenograft Model Antitumor Assays
chemical_list: D012235:Rhodamines; D017239:Paclitaxel
keywords: cancer stem cells; expansile nanoparticle; paclitaxel; pancreatic carcinomatosis; tumor localization