title: Long Non-coding RNA, PANDA, Contributes to the Stabilization of p53 Tumor Suppressor Protein.
abstract: BACKGROUND
  P21-associated noncoding RNA DNA damage-activated (PANDA) is induced in response to DNA damage and represses apoptosis by inhibiting the function of nuclear transcription factor Y subunit alpha (NF-YA) transcription factor. Herein, we report that PANDA affects regulation of p53 tumor-suppressor protein.
  
  
  METHODS
  U2OS cells were transfected with PANDA siRNAs. At 72 h post-transfection, cells were subjected to immunoblotting and quantitative reverse transcription-polymerase chain reaction.
  
  
  RESULTS
  Depletion of PANDA was associated with decreased levels of p53 protein, but not p53 mRNA. The stability of p53 protein was markedly reduced by PANDA silencing. Degradation of p53 protein by silencing PANDA was prevented by treatment of MG132, a proteasome inhibitor. Moreover, depletion of PANDA prevented accumulation of p53 protein, as a result of DNA damage, induced by the genotoxic agent etoposide.
  
  
  CONCLUSIONS
  These results suggest that PANDA stabilizes p53 protein in response to DNA damage, and provide new insight into the regulatory mechanisms of p53.
authors.affiliation: Department of Biological and Environmental Chemistry, Faculty of Humanity-Oriented Science and Engineering, Kinki University, Iizuka, Fukuoka, Japan ykotake@fuk.kindai.ac.jp.
authors.forename: Yojiro
authors.initials: Y
authors.lastname: Kotake
authors.affiliation: Department of Molecular Biology, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Shizuoka, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Kitagawa
authors.affiliation: Department of Molecular Biology, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Shizuoka, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Ohhata
authors.affiliation: Department of Molecular Biology, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Shizuoka, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Sakai
authors.affiliation: Laboratory for Promotion of Advanced Research, Advanced Research Facilities & Services, Preeminent Medical Photonics Education & Research Center, Hamamatsu University School of Medicine, Higashi ku, Hamamatsu, Shizuoka, Japan.
authors.forename: Chiharu
authors.initials: C
authors.lastname: Uchida
authors.affiliation: Department of Molecular Biology, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Shizuoka, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Niida
authors.affiliation: Department of Biological and Environmental Chemistry, Faculty of Humanity-Oriented Science and Engineering, Kinki University, Iizuka, Fukuoka, Japan.
authors.forename: Madoka
authors.initials: M
authors.lastname: Naemura
authors.affiliation: Department of Molecular Biology, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Shizuoka, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Kitagawa
mesh_terms: D045744:Cell Line, Tumor; D004249:DNA Damage; D005047:Etoposide / Q000494:pharmacology; D006801:Humans; D009153:Mutagens / Q000494:pharmacology; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*
chemical_list: D009153:Mutagens; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D016159:Tumor Suppressor Protein p53; D005047:Etoposide
keywords: Long non-coding RNA; PANDA; p53; protein degradation