title: Liprin-α1 is a regulator of vimentin intermediate filament network in the cancer cell adhesion machinery.
abstract: PPFIA1 is located at the 11q13 region, which is one of the most commonly amplified regions in several epithelial cancers including head and neck squamous cell carcinoma and breast carcinoma. Considering the location of PPFIA1 in this amplicon, we examined whether protein encoded by PPFIA1, liprin-α1, possesses oncogenic properties in relevant carcinoma cell lines. Our results indicate that liprin-α1 localizes to different adhesion and cytoskeletal structures to regulate vimentin intermediate filament network, thereby altering the invasion and growth properties of the cancer cells. In non-invasive cells liprin-α1 promotes expansive growth behavior with limited invasive capacity, whereas in invasive cells liprin-α1 has significant impact on mesenchymal cancer cell invasion in three-dimensional collagen. Current results identify liprin-α1 as a novel regulator of the tumor cell intermediate filaments with differential oncogenic properties in actively proliferating or motile cells.
authors.affiliation: Research Programs Unit, Genome-Scale Biology Research Program and Institute of Biomedicine, Medical Biochemistry and Developmental Biology, 00014 University of Helsinki, Finland.
authors.forename: Henna
authors.initials: H
authors.lastname: Pehkonen
authors.affiliation: Research Programs Unit, Genome-Scale Biology Research Program and Institute of Biomedicine, Medical Biochemistry and Developmental Biology, 00014 University of Helsinki, Finland.
authors.forename: Pernilla
authors.initials: P
authors.lastname: von Nandelstadh
authors.affiliation: Research Programs Unit, Genome-Scale Biology Research Program and Institute of Biomedicine, Medical Biochemistry and Developmental Biology, 00014 University of Helsinki, Finland.
authors.forename: Piia-Riitta
authors.initials: PR
authors.lastname: Karhemo
authors.affiliation: Research Programs Unit, Genome-Scale Biology Research Program and Institute of Biomedicine, Medical Biochemistry and Developmental Biology, 00014 University of Helsinki, Finland.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Lepikhova
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Turku University and Turku University Hospital, Finland.
authors.forename: Reidar
authors.initials: R
authors.lastname: Grenman
authors.affiliation: Research Programs Unit, Genome-Scale Biology Research Program and Institute of Biomedicine, Medical Biochemistry and Developmental Biology, 00014 University of Helsinki, Finland.|Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.|K. Albin Johansson Senior Researcher, Finnish Cancer Institute, Finland.
authors.forename: Kaisa
authors.initials: K
authors.lastname: Lehti
authors.affiliation: Research Programs Unit, Genome-Scale Biology Research Program and Institute of Biomedicine, Medical Biochemistry and Developmental Biology, 00014 University of Helsinki, Finland.
authors.forename: Outi
authors.initials: O
authors.lastname: Monni
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D006801:Humans; D007382:Intermediate Filaments / Q000378:metabolism*; D015513:Oncogene Proteins / Q000378:metabolism*; D014746:Vimentin / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D015513:Oncogene Proteins; C094398:PPFIA1 protein, human; D014746:Vimentin