title: HyperCEST detection of cucurbit[6]uril in whole blood using an ultrashort saturation Pre-pulse train.
abstract: Xenon based biosensors have the potential to detect and localize biomarkers associated with a wide variety of diseases. The development and nuclear magnetic resonance (NMR) characterization of cage molecules which encapsulate hyperpolarized xenon is imperative for the development of these xenon biosensors. We acquired (129) Xe NMR spectra, and magnetic resonance images and a HyperCEST saturation map of cucurbit[6]uril (CB6) in whole bovine blood. We observed a mean HyperCEST depletion of 84% (n = 5) at a concentration of 5 mM and 74% at 2.5 mM. Additionally, we collected these data using a pulsed HyperCEST saturation pre-pulse train with a SAR of 0.025 W/kg which will minimize any potential RF heating in animal or human tissue. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Lakehead University, Department of Chemistry, 955 Oliver Rd, Thunder Bay, ON P7B 5E1, Canada.|Thunder Bay Regional Research Institute, 980 Oliver Rd, Thunder Bay, ON P7B 5E1, Canada.
authors.forename: Francis T
authors.initials: FT
authors.lastname: Hane
authors.affiliation: Lakehead University, Department of Chemistry, 955 Oliver Rd, Thunder Bay, ON P7B 5E1, Canada.
authors.forename: Peter S
authors.initials: PS
authors.lastname: Smylie
authors.affiliation: Thunder Bay Regional Research Institute, 980 Oliver Rd, Thunder Bay, ON P7B 5E1, Canada.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: Lakehead University, Department of Chemistry, 955 Oliver Rd, Thunder Bay, ON P7B 5E1, Canada.
authors.forename: Julia
authors.initials: J
authors.lastname: Ruberto
authors.affiliation: Lakehead University, Department of Chemistry, 955 Oliver Rd, Thunder Bay, ON P7B 5E1, Canada.
authors.forename: Krista
authors.initials: K
authors.lastname: Dowhos
authors.affiliation: Thunder Bay Regional Research Institute, 980 Oliver Rd, Thunder Bay, ON P7B 5E1, Canada.|Philips Healthcare, 65 Epping Road, North Ryde, NSW 2113, Australia.
authors.forename: Iain
authors.initials: I
authors.lastname: Ball
authors.affiliation: Thunder Bay Regional Research Institute, 980 Oliver Rd, Thunder Bay, ON P7B 5E1, Canada.|University of Alberta, Department of Oncology, 11560 University Avenue, Edmonton, Alberta T6G 1Z2, Canada.
authors.forename: Boguslaw
authors.initials: B
authors.lastname: Tomanek
authors.affiliation: University of Rhode Island, Department of Chemistry, 51 Lower College Rd, Kingston, RI 02881, USA.
authors.forename: Brenton
authors.initials: B
authors.lastname: DeBoef
authors.affiliation: Lakehead University, Department of Chemistry, 955 Oliver Rd, Thunder Bay, ON P7B 5E1, Canada.|Thunder Bay Regional Research Institute, 980 Oliver Rd, Thunder Bay, ON P7B 5E1, Canada.
authors.forename: Mitchell S
authors.initials: MS
authors.lastname: Albert
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D001952:Bridged-Ring Compounds / Q000097:blood*; D002417:Cattle; D004912:Erythrocytes / Q000737:chemistry; D006801:Humans; D007093:Imidazoles / Q000097:blood*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D014978:Xenon / Q000737:chemistry*; D047028:Macrocyclic Compounds; D000099246:Cucurbit(n)urils
chemical_list: D001952:Bridged-Ring Compounds; D007093:Imidazoles; D014978:Xenon; C454805:cucurbit(6)uril; D047028:Macrocyclic Compounds; D000099246:Cucurbit(n)urils
keywords: Contrast Agents; HyperCEST; Xenon Biosensors