title: Effect of Fluoride-Containing Toothpastes on Enamel Demineralization and Streptococcus mutans Biofilm Architecture.
abstract: This study aimed to explore the effect of fluoridated toothpastes on biofilm architecture and enamel demineralization in an in vitro biofilm model. Streptococcus mutans was grown on enamel and treated with slurries of commercial toothpastes, containing SnF2 or NaF. Water and chlorhexidine were used as negative and positive controls, respectively. The developed biofilms were imaged and enamel demineralization was measured. SnF2 and NaF toothpaste treatments significantly reduced enamel demineralization, but SnF2 toothpaste was more effective. Only SnF2 toothpaste and chlorhexidine treatments caused reductions on biofilm mass and thickness. In conclusion, this biofilm model was able to differentiate the effects of the SnF2 and NaF toothpastes on biofilm architecture and enamel demineralization.
authors.affiliation: Department of Cariology, Restorative Sciences and Endodontics, School of Dentistry, University of Michigan, Ann Arbor, Mich., USA.
authors.forename: Constanza E
authors.initials: CE
authors.lastname: Fernández
authors.forename: Margherita
authors.initials: M
authors.lastname: Fontana
authors.forename: Derek
authors.initials: D
authors.lastname: Samarian
authors.forename: Jaime A
authors.initials: JA
authors.lastname: Cury
authors.forename: Alexander H
authors.initials: AH
authors.lastname: Rickard
authors.forename: Carlos
authors.initials: C
authors.lastname: González-Cabezas
mesh_terms: D000818:Animals; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002417:Cattle; D002710:Chlorhexidine / Q000494:pharmacology; D003743:Dental Enamel / Q000187:drug effects* / Q000382:microbiology / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D006863:Hydrogen-Ion Concentration; D021621:Imaging, Three-Dimensional; D066298:In Vitro Techniques; D018613:Microscopy, Confocal; D012463:Saliva / Q000378:metabolism; D012969:Sodium Fluoride / Q000008:administration & dosage / Q000494:pharmacology*; D013295:Streptococcus mutans / Q000187:drug effects* / Q000254:growth & development; D014002:Tin Fluorides / Q000008:administration & dosage / Q000494:pharmacology*; D017001:Tooth Demineralization / Q000188:drug therapy* / Q000382:microbiology / Q000517:prevention & control; D014100:Toothpastes / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D014002:Tin Fluorides; D014100:Toothpastes; D012969:Sodium Fluoride; D002710:Chlorhexidine