title: Polyphosphate is involved in cell cycle progression and genomic stability in Saccharomyces cerevisiae.
abstract: Polyphosphate (polyP) is a linear chain of up to hundreds of inorganic phosphate residues that is necessary for many physiological functions in all living organisms. In some bacteria, polyP supplies material to molecules such as DNA, thus playing an important role in biosynthetic processes in prokaryotes. In the present study, we set out to gain further insight into the role of polyP in eukaryotic cells. We observed that polyP amounts are cyclically regulated in Saccharomyces cerevisiae, and those mutants that cannot synthesise (vtc4Δ) or hydrolyse polyP (ppn1Δ, ppx1Δ) present impaired cell cycle progression. Further analysis revealed that polyP mutants show delayed nucleotide production and increased genomic instability. Based on these findings, we concluded that polyP not only maintains intracellular phosphate concentrations in response to fluctuations in extracellular phosphate levels, but also muffles internal cyclic phosphate fluctuations, such as those produced by the sudden demand of phosphate to synthetize deoxynucleotides just before and during DNA duplication. We propose that the presence of polyP in eukaryotic cells is required for the timely and accurate duplication of DNA.
authors.affiliation: Faculty of Medicine and Health Sciences, Universitat Internacional de Catalunya, Barcelona, Spain.
authors.forename: Samuel
authors.initials: S
authors.lastname: Bru
authors.affiliation: Faculty of Medicine and Health Sciences, Universitat Internacional de Catalunya, Barcelona, Spain.
authors.forename: Joan Marc
authors.initials: JM
authors.lastname: Martínez-Laínez
authors.affiliation: Faculty of Medicine and Health Sciences, Universitat Internacional de Catalunya, Barcelona, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Hernández-Ortega
authors.affiliation: Faculty of Medicine and Health Sciences, Universitat Internacional de Catalunya, Barcelona, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Quandt
authors.affiliation: Research Group on Neuromuscular and Mitochondrial Disorders, Vall d'Hebron Institut de Recerca, Universitat Autònoma de Barcelona, Barcelona, Spain.|Biomedical Network Research Centre on Rare Diseases (CIBERER), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Torres-Torronteras
authors.affiliation: Research Group on Neuromuscular and Mitochondrial Disorders, Vall d'Hebron Institut de Recerca, Universitat Autònoma de Barcelona, Barcelona, Spain.|Biomedical Network Research Centre on Rare Diseases (CIBERER), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Ramón
authors.initials: R
authors.lastname: Martí
authors.affiliation: Department of Biochemistry and Molecular Biology and the Institute of Biotechnology and Biomedicine, Universitat Autònoma de Barcelona, Barcelona, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Canadell
authors.affiliation: Department of Biochemistry and Molecular Biology and the Institute of Biotechnology and Biomedicine, Universitat Autònoma de Barcelona, Barcelona, Spain.
authors.forename: Joaquin
authors.identifier: 0000-0002-6774-2987
authors.initials: J
authors.lastname: Ariño
authors.affiliation: Department of Medical Biochemistry and Biophysics, Umeå University, Sweden.
authors.forename: Sushma
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Faculty of Medicine and Health Sciences, Universitat Internacional de Catalunya, Barcelona, Spain.
authors.forename: Javier
authors.identifier: 0000-0002-0402-4427
authors.initials: J
authors.lastname: Jiménez
authors.affiliation: Faculty of Medicine and Health Sciences, Universitat Internacional de Catalunya, Barcelona, Spain.
authors.forename: Josep
authors.initials: J
authors.lastname: Clotet
mesh_terms: D059447:Cell Cycle Checkpoints / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D042822:Genomic Instability; D015388:Organelles / Q000378:metabolism; D011122:Polyphosphates / Q000378:metabolism*; D011387:Prokaryotic Cells / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000378:metabolism*
chemical_list: D011122:Polyphosphates