title: PON2 and ATP2B2 gene polymorphisms with noise-induced hearing loss.
abstract: BACKGROUND
  Noise-induced hearing loss (NIHL) is a complex disease induced by a combination of genetic and environmental factors. Paraoxonase2 (PON2) gene involved in the regulation of reactive oxygen species, and affecting the vulnerability of cochlea to NIHL, and ATPase, calcium-transporting, plasma membrane 2 (ATP2B2) gene which encodes plasma membrane calcium-transporting ATPase isoform 2 (PMCA2) are the candidate genes relating to the attack of NIHL. In this study, we investigated whether ATP2B2 and PON2 polymorphisms were associated with NIHL in Chinese of Han nationality population.
  
  
  METHODS
  We performed a case-control study between six single nucleotide polymorphisms (SNPs) (rs1719571, rs3209637 and rs4327369 within ATP2B2, rs12026, rs7785846 and rs12704796 within PON2) and NIHL in 454 subjects. All the SNPs were genotypes, using the TaqMan MGB probe assay. Odds ratios (ORs) were calculated with 95% confidence intervals (95% CIs) with logistic regression analysis to test the level of association for SNPs.
  
  
  RESULTS
  In our study, 221 subjects with hearing loss and 233 subjects without hearing loss were recruited. The frequencies of the CG and CG + GG genotype of rs12026 (PON2) conferred risk factors for NIHL with adjusted OR values of 2.62 (95% CI, 1.69-4.06) and 2.48 (95% CI, 1.63-3.78), respectively. This kind of significance was also found at locus rs7785846, where genotypes CT and CT + TT were the risk types, with adjusted ORs of 2.52 (95% CI, 1.62-3.93) and 2.35 (95% CI, 1.54-3.58), respectively. We performed stratified analysis per noise exposure level, when it came to rs7785846 and rs12026 in the >92 dB(A) noise exposure group, the subjects who carried heterozygote were of significantly (P<0.01) higher susceptibility to NIHL than homozygote carriers. By contrast, no significantly higher risk was found for any rs12704796 genotypes or any genotypes in ATP2B2 (P>0.05), which may suggest that these SNPs did not have significant effects on noise susceptibility across noise exposure.
  
  
  CONCLUSIONS
  Our research suggested that PON2 might play a role in the etiology of NIHL in Chinese of Han nationality population.
authors.affiliation: 1 Key Lab of Modern Toxicology, Ministry of Education, Department of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China ; 2 Department of Science and Education, Nanjing Prevention and Treatment Center for Occupational Diseases, Nanjing, China ; 3 Center of Prevention and Health Care, the Forth People's Hospital of Kunshan City, Suzhou, China ; 4 Ministry of Environmental Protection, Nanjing Institute of Environmental Sciences/Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Nanjing, China ; 5 Department of ENT, Huai'an Second People's Hospital, Huai'an, China ; 6 Institute of Occupational Disease Prevention, Jiangsu Provincial Center for Disease Prevention and Control, Nanjing, China.
authors.forename: Xiuting
authors.initials: X
authors.lastname: Li
authors.affiliation: 1 Key Lab of Modern Toxicology, Ministry of Education, Department of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China ; 2 Department of Science and Education, Nanjing Prevention and Treatment Center for Occupational Diseases, Nanjing, China ; 3 Center of Prevention and Health Care, the Forth People's Hospital of Kunshan City, Suzhou, China ; 4 Ministry of Environmental Protection, Nanjing Institute of Environmental Sciences/Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Nanjing, China ; 5 Department of ENT, Huai'an Second People's Hospital, Huai'an, China ; 6 Institute of Occupational Disease Prevention, Jiangsu Provincial Center for Disease Prevention and Control, Nanjing, China.
authors.forename: Jinglian
authors.initials: J
authors.lastname: Cao
authors.affiliation: 1 Key Lab of Modern Toxicology, Ministry of Education, Department of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China ; 2 Department of Science and Education, Nanjing Prevention and Treatment Center for Occupational Diseases, Nanjing, China ; 3 Center of Prevention and Health Care, the Forth People's Hospital of Kunshan City, Suzhou, China ; 4 Ministry of Environmental Protection, Nanjing Institute of Environmental Sciences/Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Nanjing, China ; 5 Department of ENT, Huai'an Second People's Hospital, Huai'an, China ; 6 Institute of Occupational Disease Prevention, Jiangsu Provincial Center for Disease Prevention and Control, Nanjing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: 1 Key Lab of Modern Toxicology, Ministry of Education, Department of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China ; 2 Department of Science and Education, Nanjing Prevention and Treatment Center for Occupational Diseases, Nanjing, China ; 3 Center of Prevention and Health Care, the Forth People's Hospital of Kunshan City, Suzhou, China ; 4 Ministry of Environmental Protection, Nanjing Institute of Environmental Sciences/Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Nanjing, China ; 5 Department of ENT, Huai'an Second People's Hospital, Huai'an, China ; 6 Institute of Occupational Disease Prevention, Jiangsu Provincial Center for Disease Prevention and Control, Nanjing, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Song
authors.affiliation: 1 Key Lab of Modern Toxicology, Ministry of Education, Department of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China ; 2 Department of Science and Education, Nanjing Prevention and Treatment Center for Occupational Diseases, Nanjing, China ; 3 Center of Prevention and Health Care, the Forth People's Hospital of Kunshan City, Suzhou, China ; 4 Ministry of Environmental Protection, Nanjing Institute of Environmental Sciences/Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Nanjing, China ; 5 Department of ENT, Huai'an Second People's Hospital, Huai'an, China ; 6 Institute of Occupational Disease Prevention, Jiangsu Provincial Center for Disease Prevention and Control, Nanjing, China.
authors.forename: Guixiang
authors.initials: G
authors.lastname: Ji
authors.affiliation: 1 Key Lab of Modern Toxicology, Ministry of Education, Department of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China ; 2 Department of Science and Education, Nanjing Prevention and Treatment Center for Occupational Diseases, Nanjing, China ; 3 Center of Prevention and Health Care, the Forth People's Hospital of Kunshan City, Suzhou, China ; 4 Ministry of Environmental Protection, Nanjing Institute of Environmental Sciences/Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Nanjing, China ; 5 Department of ENT, Huai'an Second People's Hospital, Huai'an, China ; 6 Institute of Occupational Disease Prevention, Jiangsu Provincial Center for Disease Prevention and Control, Nanjing, China.
authors.forename: Qiu
authors.initials: Q
authors.lastname: Dong
authors.affiliation: 1 Key Lab of Modern Toxicology, Ministry of Education, Department of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China ; 2 Department of Science and Education, Nanjing Prevention and Treatment Center for Occupational Diseases, Nanjing, China ; 3 Center of Prevention and Health Care, the Forth People's Hospital of Kunshan City, Suzhou, China ; 4 Ministry of Environmental Protection, Nanjing Institute of Environmental Sciences/Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Nanjing, China ; 5 Department of ENT, Huai'an Second People's Hospital, Huai'an, China ; 6 Institute of Occupational Disease Prevention, Jiangsu Provincial Center for Disease Prevention and Control, Nanjing, China.
authors.forename: Chunlong
authors.initials: C
authors.lastname: Wei
authors.affiliation: 1 Key Lab of Modern Toxicology, Ministry of Education, Department of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China ; 2 Department of Science and Education, Nanjing Prevention and Treatment Center for Occupational Diseases, Nanjing, China ; 3 Center of Prevention and Health Care, the Forth People's Hospital of Kunshan City, Suzhou, China ; 4 Ministry of Environmental Protection, Nanjing Institute of Environmental Sciences/Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Nanjing, China ; 5 Department of ENT, Huai'an Second People's Hospital, Huai'an, China ; 6 Institute of Occupational Disease Prevention, Jiangsu Provincial Center for Disease Prevention and Control, Nanjing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Cao
authors.affiliation: 1 Key Lab of Modern Toxicology, Ministry of Education, Department of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China ; 2 Department of Science and Education, Nanjing Prevention and Treatment Center for Occupational Diseases, Nanjing, China ; 3 Center of Prevention and Health Care, the Forth People's Hospital of Kunshan City, Suzhou, China ; 4 Ministry of Environmental Protection, Nanjing Institute of Environmental Sciences/Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Nanjing, China ; 5 Department of ENT, Huai'an Second People's Hospital, Huai'an, China ; 6 Institute of Occupational Disease Prevention, Jiangsu Provincial Center for Disease Prevention and Control, Nanjing, China.
authors.forename: Boshen
authors.initials: B
authors.lastname: Wang
authors.affiliation: 1 Key Lab of Modern Toxicology, Ministry of Education, Department of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China ; 2 Department of Science and Education, Nanjing Prevention and Treatment Center for Occupational Diseases, Nanjing, China ; 3 Center of Prevention and Health Care, the Forth People's Hospital of Kunshan City, Suzhou, China ; 4 Ministry of Environmental Protection, Nanjing Institute of Environmental Sciences/Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Nanjing, China ; 5 Department of ENT, Huai'an Second People's Hospital, Huai'an, China ; 6 Institute of Occupational Disease Prevention, Jiangsu Provincial Center for Disease Prevention and Control, Nanjing, China.
authors.forename: Baoli
authors.initials: B
authors.lastname: Zhu
authors.affiliation: 1 Key Lab of Modern Toxicology, Ministry of Education, Department of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China ; 2 Department of Science and Education, Nanjing Prevention and Treatment Center for Occupational Diseases, Nanjing, China ; 3 Center of Prevention and Health Care, the Forth People's Hospital of Kunshan City, Suzhou, China ; 4 Ministry of Environmental Protection, Nanjing Institute of Environmental Sciences/Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Nanjing, China ; 5 Department of ENT, Huai'an Second People's Hospital, Huai'an, China ; 6 Institute of Occupational Disease Prevention, Jiangsu Provincial Center for Disease Prevention and Control, Nanjing, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Xiao
keywords: ATPase, calcium-transporting, plasma membrane 2 (ATP2B2); noise-induced hearing loss (NIHL); paraoxonase2 (PON2); polymorphism