title: Orderly nucleic acid aggregates by electrostatic self-assembly in single cells for miRNA detection and visualizing.
abstract: Orderly nucleic acid aggregates (ONAAs) self-assembled on mesoporous silica nanoparticles (MSNs) with positively charged aminopropyl groups (PC) were firstly developed. Interestingly, a novel electrostatic DNA self-assembly could realize hybridization chain reaction (HCR) on the surface of PCMSNs in single cells. Significantly, a non-destructive amplification strategy based on ONAAs-PCMSNs was successfully developed for miRNA detection and in situ imaging by the prominent and agminated fluorescence-bright spots in living cells.
authors.affiliation: Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Shandong Normal University, Jinan 250014, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Shandong Province Key Laboratory of Detection Technology for Tumor Makers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276000, China. zhangzhen19801981@126.com.
authors.forename: Zhaopeng
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Shandong Province Key Laboratory of Detection Technology for Tumor Makers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276000, China. zhangzhen19801981@126.com.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Shandong Normal University, Jinan 250014, China and Shandong Province Key Laboratory of Detection Technology for Tumor Makers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276000, China. zhangzhen19801981@126.com.
authors.forename: Rui
authors.initials: R
authors.lastname: Ren
authors.affiliation: Shandong Province Key Laboratory of Detection Technology for Tumor Makers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276000, China. zhangzhen19801981@126.com.
authors.forename: Shusheng
authors.initials: S
authors.lastname: Zhang
mesh_terms: D045744:Cell Line, Tumor; D004247:DNA; D006801:Humans; D035683:MicroRNAs / Q000032:analysis*; D053758:Nanoparticles; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000737:chemistry*; D059010:Single-Cell Analysis; D055672:Static Electricity
chemical_list: D035683:MicroRNAs; D009696:Nucleic Acids; D004247:DNA