title: Development and application of a comprehensive lipidomic analysis to investigate Tripterygium wilfordii-induced liver injury.
abstract: Lipid metabolic pathways play pivotal roles in liver function, and disturbances of these pathways are associated with various diseases. Thus, comprehensive characterization and measurement of lipid metabolites are essential to deciphering the contributions of lipid network metabolism to diseases or its responses to drug intervention. Here, we report an integrated lipidomic analysis for the comprehensive detection of lipid metabolites. To facilitate the characterization of untargeted lipids through fragmentation analysis, nine formulas were proposed to identify the fatty acid composition of lipids from complex MS (n) spectrum information. By these formulas, the co-eluted isomeric compounds could be distinguished. In total, 250 lipids were detected and characterized, including diacylglycerols, triacylglycerols, glycerophosphoethanolamines, glycerophosphocholines, glycerophosphoserines, glycerophosphoglycerols, glycerophosphoinositols, cardiolipins, ceramides, and sphingomyelins. Integrated with the targeted lipidomics, a total of 27 inflammatory oxylipins were also measured. To evaluate the aberrant lipid metabolism involved in liver injury induced by Tripterygium wilfordii, lipid network metabolism was further investigated. Results indicated that energy lipid modification, membrane remodeling, potential signaling lipid alterations, and abnormal inflammation response were associated with injury. Because of the important roles of lipids in liver metabolism, this new method is expected to be useful in analyzing other lipid metabolism diseases.
authors.affiliation: Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210023, China. sunnyxyl1021@163.com.
authors.forename: Tong
authors.initials: T
authors.lastname: Xie
authors.affiliation: The First Clinical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210023, China.
authors.forename: Xueping
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210023, China.
authors.forename: Shouchuan
authors.initials: S
authors.lastname: Wang
authors.affiliation: The First Clinical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210023, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Jiangsu Engineering Research Center for Efficient Delivery System of TCM, College of Pharmacy, Nanjing University of Chinese Medicine, No. 138 Xianlin Avenue, Nanjing, Jiangsu, 210023, China.
authors.forename: Huaxu
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Jiangsu Engineering Research Center for Efficient Delivery System of TCM, College of Pharmacy, Nanjing University of Chinese Medicine, No. 138 Xianlin Avenue, Nanjing, Jiangsu, 210023, China.
authors.forename: An
authors.initials: A
authors.lastname: Kang
authors.affiliation: Jiangsu Engineering Research Center for Efficient Delivery System of TCM, College of Pharmacy, Nanjing University of Chinese Medicine, No. 138 Xianlin Avenue, Nanjing, Jiangsu, 210023, China.
authors.forename: Haishan
authors.initials: H
authors.lastname: Deng
authors.affiliation: Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210023, China.
authors.forename: Jianya
authors.initials: J
authors.lastname: Xu
authors.affiliation: Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210023, China.
authors.forename: Cunsi
authors.initials: C
authors.lastname: Shen
authors.affiliation: Jiangsu Engineering Research Center for Efficient Delivery System of TCM, College of Pharmacy, Nanjing University of Chinese Medicine, No. 138 Xianlin Avenue, Nanjing, Jiangsu, 210023, China.
authors.forename: Liuqing
authors.initials: L
authors.lastname: Di
authors.affiliation: Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210023, China. jshan@njutcm.edu.cn.
authors.forename: Jinjun
authors.initials: J
authors.lastname: Shan
mesh_terms: D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000378:metabolism / Q000633:toxicity*; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects; D008055:Lipids / Q000737:chemistry*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D055432:Metabolomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D029757:Tripterygium / Q000378:metabolism / Q000633:toxicity*
chemical_list: D004365:Drugs, Chinese Herbal; D008055:Lipids
keywords: Lipidomics; Liver injury; Mass spectrometry; Oxylipins; Tripterygium wilfordii