title: Urinary Albumin Detection: Comparison of Two Different Methods.
abstract: BACKGROUND
  Monitoring urinary albumin is a useful method in clinical practice for the management of diabetic nephropathy, chronic kidney disease, and hypertension. Currently there are neither standardized methods nor reference material for the determination of urinary albumin; for this reason it is useful to compare different assays used in clinical laboratory.
  
  
  OBJECTIVE
  The aim of this study is to verify analytical performance of an immunoturbidimetric assay on Roche Cobas 8000 platform and to compare urinary albumin results with those obtained by immunonephelometry on Siemens Dade Behring BN II Nephelometer.
  
  
  RESULTS
  The method comparison showed a good linear relationship, confirmed by Passing-Bablok and Bland-Altman plots. The turbidimetric assay meets the requirements of accuracy and precision for the practice of medical diagnostics and clinical use.
  
  
  CONCLUSIONS
  The present study can contribute to the methods standardization and harmonization of urinary albumin assay.
authors.affiliation: Department of Clinical Biochemistry, "A. Gemelli" Policlinico Universitario, Rome, Italy. rossanamolinario@libero.it.
authors.forename: Rossana
authors.initials: R
authors.lastname: Molinario
authors.affiliation: Department of Clinical Biochemistry, "A. Gemelli" Policlinico Universitario, Rome, Italy.
authors.forename: Krizia
authors.initials: K
authors.lastname: Pocino
authors.affiliation: Department of Clinical Biochemistry, "A. Gemelli" Policlinico Universitario, Rome, Italy.
authors.forename: Pio Dante
authors.initials: PD
authors.lastname: Daloiso
authors.affiliation: Department of Clinical Biochemistry, "A. Gemelli" Policlinico Universitario, Rome, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Giannace
authors.affiliation: Department of Clinical Biochemistry, "A. Gemelli" Policlinico Universitario, Rome, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Spirito
authors.affiliation: Department of Clinical Biochemistry, "A. Gemelli" Policlinico Universitario, Rome, Italy.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Zuppi
authors.affiliation: Department of Clinical Biochemistry, "A. Gemelli" Policlinico Universitario, Rome, Italy.
authors.forename: Mirca
authors.initials: M
authors.lastname: Antenucci
mesh_terms: D000419:Albuminuria / Q000175:diagnosis* / Q000652:urine; D003928:Diabetic Nephropathies / Q000652:urine*; D006801:Humans; D006973:Hypertension / Q000652:urine*; D007118:Immunoassay / Q000379:methods*; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D012044:Regression Analysis; D051436:Renal Insufficiency, Chronic / Q000652:urine*
keywords: method evaluation; urinary albumin