title: Engineering human cells and tissues through pluripotent stem cells.
abstract: The utility of human pluripotent stem cells (hPSCs) depends on their ability to produce functional cells and tissues of the body. Two strategies have been developed: directed differentiation of enriched populations of cells that match a regional and functional profile and spontaneous generation of three-dimensional organoids that resemble tissues in the body. Genomic editing of hPSCs and their differentiated cells broadens the use of the hPSC paradigm in studying human cellular function and disease as well as developing therapeutics.
authors.affiliation: Waisman Center, University of Wisconsin, Madison, WI 53705, United States.
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Jones
authors.affiliation: Waisman Center, University of Wisconsin, Madison, WI 53705, United States; Department of Neuroscience, University of Wisconsin, Madison, WI 53705, United States; Department of Neurology, University of Wisconsin, Madison, WI 53705, United States. Electronic address: suchun.zhang@wisc.edu.
authors.forename: Su-Chun
authors.initials: SC
authors.lastname: Zhang
mesh_terms: D002454:Cell Differentiation; D006801:Humans; D039904:Pluripotent Stem Cells / Q000166:cytology*; D017393:RNA Editing / Q000235:genetics*; D023822:Tissue Engineering / Q000379:methods*