title: Rutin protects rat articular chondrocytes against oxidative stress induced by hydrogen peroxide through SIRT1 activation.
abstract: The progressive degeneration and ossification of articular chondrocytes are main symptoms in the pathogenesis of osteoarthritis (OA). Several flavonoids may provide an adjunctive alternative for the management of moderate OA in humans. Rutin, a natural flavone derivative (quercetin-3-rhamnosylglucoside), is well known for its potent anti-inflammatory and anti-oxidant properties against oxidative stress. However, the protective function of rutin related to OA, which is characterized by deterioration of articular cartilage, remains unclear. The present study investigated the protective effects of rutin, an activator of silent information regulator 1 (SIRT1), involved in the inhibition of NF-κB/MAPK signaling pathway in hydrogen peroxide (H2O2)-induced oxidative stress in rat chondrocytes. SIRT1 activation by rutin attenuated levels of inflammatory cytokines and NF-κB/MAPK signaling, whereas the inhibition of SIRT1 by sirtinol counteracted the beneficial effects of rutin in H2O2-treated chondrocytes. The findings of these studies suggested the potential involvement of SIRT1 in the pathogenesis of OA, and indicated that rutin is a possible therapeutic option for OA.
authors.affiliation: Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University, 79 Gobongro, Iksan, 570-752, Republic of Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Na
authors.affiliation: Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University, 79 Gobongro, Iksan, 570-752, Republic of Korea.
authors.forename: Kibbeum
authors.initials: K
authors.lastname: Song
authors.affiliation: Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University, 79 Gobongro, Iksan, 570-752, Republic of Korea. Electronic address: raios@hanmail.net.
authors.forename: Sokho
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University, 79 Gobongro, Iksan, 570-752, Republic of Korea. Electronic address: jkwon@chonbuk.ac.kr.
authors.forename: Jungkee
authors.initials: J
authors.lastname: Kwon
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects / Q000502:physiology*; D016207:Cytokines / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000008:administration & dosage*; D007596:Joints / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D020935:MAP Kinase Signaling System / Q000187:drug effects / Q000502:physiology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012431:Rutin / Q000008:administration & dosage*; D056564:Sirtuin 1 / Q000378:metabolism*
chemical_list: D016207:Cytokines; D012431:Rutin; D006861:Hydrogen Peroxide; C488368:Sirt1 protein, rat; D056564:Sirtuin 1
keywords: Chondrocytes; Osteoarthritis; Oxidation; Rutin; SIRT1