title: Mycobacterium tuberculosis Zinc Metalloprotease-1 Elicits Tuberculosis-Specific Humoral Immune Response Independent of Mycobacterial Load in Pulmonary and Extra-Pulmonary Tuberculosis Patients.
abstract: Conventionally, facultative intracellular pathogen, Mycobacterium tuberculosis, the tuberculosis (TB) causing bacilli in human is cleared by cell-mediated immunity (CMI) with CD4(+) T cells playing instrumental role in protective immunity, while antibody-mediated immunity (AMI) is considered non-protective. This longstanding convention has been challenged with recent evidences of increased susceptibility of hosts with compromised AMI and monoclonal antibodies conferring passive protection against TB and other intracellular pathogens. Therefore, novel approaches toward vaccine development include strategies aiming at induction of humoral response along with CMI. This necessitates the identification of mycobacterial proteins with properties of immunomodulation and strong immunogenicity. In this study, we determined the immunogenic potential of M. tuberculosis Zinc metalloprotease-1 (Zmp1), a secretory protein essential for intracellular survival and pathogenesis of M. tuberculosis. We observed that Zmp1 was secreted by in vitro grown M. tuberculosis under granuloma-like stress conditions (acidic, oxidative, iron deficiency, and nutrient deprivation) and generated Th2 cytokine microenvironment upon exogenous treatment of peripheral blood mononulear cells PBMCs with recombinant Zmp1 (rZmp1). This was supported by recording specific and robust humoral response in TB patients in a cohort of 295. The anti-Zmp1 titers were significantly higher in TB patients (n = 121) as against healthy control (n = 62), household contacts (n = 89) and non-specific infection controls (n = 23). A significant observation of the study is the presence of equally high titers of anti-Zmp1 antibodies in a range of patients with high bacilli load (sputum bacilli load of 300+ per mL) to paucibacillary smear-negative pulmonary tuberculosis (PTB) cases. This clearly indicated the potential of Zmp1 to evoke an effective humoral response independent of mycobacterial load. Such mycobacterial proteins can be explored as antigen candidates for prime-boost vaccination strategies or extrapolated as markers for disease detection and progression.
authors.affiliation: Department of Biochemistry, School of Life Sciences, University of Hyderabad Hyderabad, India.
authors.forename: Mani H
authors.initials: MH
authors.lastname: Vemula
authors.affiliation: Department of Biochemistry, School of Life Sciences, University of Hyderabad Hyderabad, India.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Ganji
authors.affiliation: Department of Immunology, Bhagwan Mahavir Medical Research Center Hyderabad, India.
authors.forename: Ramya
authors.initials: R
authors.lastname: Sivangala
authors.affiliation: Department of Biochemistry, School of Life Sciences, University of Hyderabad Hyderabad, India.
authors.forename: Kiran
authors.initials: K
authors.lastname: Jakkala
authors.affiliation: Department of Immunology, Bhagwan Mahavir Medical Research CenterHyderabad, India; Department of Genetics, Osmania UniversityHyderabad, India.
authors.forename: Sumanlatha
authors.initials: S
authors.lastname: Gaddam
authors.affiliation: Free Chest Clinic, PPM DOTS, Mahavir Hospital and Research Centre Hyderabad, India.
authors.forename: Sitaramaraju
authors.initials: S
authors.lastname: Penmetsa
authors.affiliation: Department of Biochemistry, School of Life Sciences, University of Hyderabad Hyderabad, India.
authors.forename: Sharmistha
authors.initials: S
authors.lastname: Banerjee
keywords: Rv0198c; extra-pulmonary tuberculosis (EPTB); humoral immunity; pulmonary tuberculosis (PTB); tuberculosis (TB)