title: [Detection of chromosome aneuploidies in spontaneous abortion villus samples by quantitative fluorescence PCR].
abstract: OBJECTIVE
  To assess the value of quantitative fluorescence polymerase chain reaction (QF-PCR) for the detection of chromosomal aneuploidies in chorionic villus samples from early abortion.
  
  
  METHODS
  One hundred seventy seven specimens were collected. Genomic DNA was extracted, and aneuploidies of 8 chromosomes (13, 15, 16, 18, 21, 22, X and Y) were detected by QF-PCR analysis.
  
  
  RESULTS
  The QF-PCR was successful in 176 (99.4%) of the cases. All detection was completed in 48 hours. Sixty three(35.8%) cases have shown abnormal signals, which included 3 cases of trisomy 13, 3 cases of trisomy 15, 14 cases of trisomy 16, 2 cases of trisomy 18, 7 cases of trisomy 22, 3 cases of trisomy 21, 13 cases of 45,X, 1 case of 47,XXX, 2 cases of 47,XXY, 2 cases of haploidy, 11 cases of triploidy, 1 case of trisomy 16 and trisomy 22, 1 case of trisomy 21 and trisomy 22. Trisomy 16 was the most common chromosome aneuploidy (22.22%), which was followed by 45,X (20.63%), triploidy (17.46%) and trisomy 22 (11.11%).
  
  
  CONCLUSIONS
  QF-PCR is a quick and easy method for detecting chromosomal aneuploidies in chorionic villi tissue. The results can provide important information for genetic counseling for spontaneous abortions.
authors.affiliation: Center of Prenatal Diagnosis, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China. kongxd@263.net.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Wu
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.forename: Yongjiang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Zhao
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Kong
mesh_terms: D000022:Abortion, Spontaneous / Q000175:diagnosis / Q000235:genetics*; D000328:Adult; D000782:Aneuploidy; D002824:Chorionic Villi / Q000737:chemistry; D005260:Female; D005453:Fluorescence; D006801:Humans; D011247:Pregnancy; D011296:Prenatal Diagnosis; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*