title: A Study of Anti Beta-2 Glycoprotein I and Anti-Prothrombin Antibodies in Patients with Unexplained Recurrent Pregnancy Losses.
abstract: To compare the levels of IgG and IgM anti beta-2 glycoprotein I antibodies and IgG and IgM anti prothrombin antibodies among women with unexplained recurrent pregnancy losses and women with at least 2 live issues. To compare the prevalence of newer anti beta-2 glycoprotein I & anti prothrombin antibodies with conventional Lupus anticoagulant & anticardiolipin antibodies. 50 women with recurrent pregnancy losses & 50 matched controls were evaluated for the presence of: Lupus anticoagulant-screened by LA sensitive aPTT& DRVV and confirmatory Staclot Assay. ELISA kits were used for detecting IgG & IgM anticardiolipin, anti beta-2 glycoprotein I & anti prothrombin antibodies. 11/50 (22 %) women in study group and none in control group had circulating antiphospholipid antibodies. 2 cases (4 %) had lupus anticoagulant. 1 case (2 %) had anticardiolipin antibody & 6 cases (12 %) were positive for anti beta-2 Glycoprotein I antibody (p value = 0.027). 3 cases (6 %) had anti prothrombin antibody. All were mutually exclusive except for one. Women with recurrent pregnancy losses should be tested for anti beta-2 Glycoprotein I antibodies & anti prothrombin antibodies in addition to conventional lupus anticoagulant and anticardiolipin antibodies. This approach can decrease the incidence of SNAP (seronegative antiphospholipid syndrome) cases while establishing the true prevalence of antiphospholipid syndrome.
authors.affiliation: Department of Pathology, Lady Hardinge Medical College and Associated Hospitals, New Delhi, 110001 India.
authors.forename: Angad
authors.initials: A
authors.lastname: Singh
authors.affiliation: Department of Pathology, Lady Hardinge Medical College and Associated Hospitals, New Delhi, 110001 India.
authors.forename: Anita
authors.initials: A
authors.lastname: Nangia
authors.affiliation: Department of Pathology, Lady Hardinge Medical College and Associated Hospitals, New Delhi, 110001 India.
authors.forename: Sunita
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Department of Obstetrics & Gynaecology, Lady Hardinge Medical College and Associated Hospitals, New Delhi, 110001 India.
authors.forename: Manju
authors.initials: M
authors.lastname: Puri
keywords: Anticardiolipin; Antiphospholipid syndrome; Beta 2-glycoprotein I; Lupus coagulation inhibitor; Pregnancy; Prothrombin