title: Establishment of a ternary network system for evaluating the antioxidant fraction of Danhong injection.
abstract: Oxidative stress plays a crucial role in numerous cardiovascular diseases. As an effective therapy, Danhong injection (DHI) is considered to act through an antioxidant mechanism for the treatment of cardiovascular disease. In our study, we focused on the potential contribution of the antioxidant capacity of DHI fractions (Frs) and established an innovative screening method based on a 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity assay. A ternary network evaluation system, which was constructed based on the radical scavenging activity, the area under the activity-concentration curve and the solid content of the fractions, was implemented to select the fractions that posed the greatest antioxidant effect. As a result, Frs 5-7 and Frs 17-19 were shown to exhibit superior antioxidant activity according to the regression area of the ternary network, which was >0.5. Furthermore, the active fractions were characterized by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry combined with nuclear magnetic resonance. This study provided an effective method for the comprehensive evaluation of the antioxidant effect of DHI fractions. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, People's Republic of China.|Research and Development Center of TCM, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, 300457, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, People's Republic of China.|Research and Development Center of TCM, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, 300457, People's Republic of China.
authors.forename: Zhenzuo
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, People's Republic of China.|Research and Development Center of TCM, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, 300457, People's Republic of China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, People's Republic of China.|Research and Development Center of TCM, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, 300457, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Chai
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, People's Republic of China.|Research and Development Center of TCM, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, 300457, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, People's Republic of China.|Research and Development Center of TCM, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, 300457, People's Republic of China.
authors.forename: Xiaoya
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, People's Republic of China.|Research and Development Center of TCM, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, 300457, People's Republic of China.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, People's Republic of China.|Research and Development Center of TCM, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, 300457, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Shandong Danhong Pharmaceutical Co. Ltd., Shandong, 712000, People's Republic of China.
authors.forename: Buchang
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Shandong Danhong Pharmaceutical Co. Ltd., Shandong, 712000, People's Republic of China.
authors.forename: Ke
authors.initials: K
authors.lastname: Qian
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, People's Republic of China.|Research and Development Center of TCM, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, 300457, People's Republic of China.
authors.forename: Yuefei
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry / Q000379:methods
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; C540581:danhong
keywords: Danhong injection; NMR; UPLC-Q/TOF MS; antioxidant activity; ternary network evaluation system