title: TRPV3 expression and vasodilator function in isolated uterine radial arteries from non-pregnant and pregnant rats.
abstract: This study investigated the expression and function of transient receptor potential vanilloid type-3 ion channels (TRPV3) in uterine radial arteries isolated from non-pregnant and twenty-day pregnant rats. Immunohistochemistry (IHC) suggested TRPV3 is primarily localized to the smooth muscle in arteries from both non-pregnant and pregnant rats. IHC using C' targeted antibody, and qPCR of TRPV3 mRNA, suggested pregnancy increased arterial TRPV3 expression. The TRPV3 activator carvacrol caused endothelium-independent dilation of phenylephrine-constricted radial arteries, with no difference between vessels from non-pregnant and pregnant animals. Carvacrol-induced dilation was reduced by the TRPV3-blockers isopentenyl pyrophosphate and ruthenium red, but not by the TRPA1 or TRPV4 inhibitors HC-030031 or HC-067047, respectively. In radial arteries from non-pregnant rats only, inhibition of NOS and sGC, or PKG, enhanced carvacrol-mediated vasodilation. Carvacrol-induced dilation of arteries from both non-pregnant and pregnant rats was prevented by the IKCa blocker TRAM-34. TRPV3 caused an endothelium-independent, IKCa-mediated dilation of the uterine radial artery. NO-PKG-mediated modulation of TRPV3 activity is lost in pregnancy, but this did not alter the response to carvacrol.
authors.affiliation: Department of Physiology, School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia. Electronic address: tim.murphy@unsw.edu.au.
authors.forename: Timothy V
authors.initials: TV
authors.lastname: Murphy
authors.affiliation: Department of Physiology, School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
authors.forename: Arjna
authors.initials: A
authors.lastname: Kanagarajah
authors.affiliation: Department of Physiology, School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
authors.forename: Sianne
authors.initials: S
authors.lastname: Toemoe
authors.affiliation: Department of Physiology, School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
authors.forename: Paul P
authors.initials: PP
authors.lastname: Bertrand
authors.affiliation: Department of Physiology, School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
authors.forename: T Hilton
authors.initials: TH
authors.lastname: Grayson
authors.affiliation: Department of Physiology, School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
authors.forename: Fiona C
authors.initials: FC
authors.lastname: Britton
authors.affiliation: School of Women's and Children's Health, University of New South Wales, Sydney, NSW 2052, Australia.
authors.forename: Leo
authors.initials: L
authors.lastname: Leader
authors.affiliation: Department of Physiology, School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
authors.forename: Sevvandi
authors.initials: S
authors.lastname: Senadheera
authors.affiliation: Department of Physiology, School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia; Inflammation and Healing Cluster, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore, QLD 4558, Australia.
authors.forename: Shaun L
authors.initials: SL
authors.lastname: Sandow
mesh_terms: D000818:Animals; D001794:Blood Pressure; D017869:Cyclic GMP-Dependent Protein Kinases / Q000378:metabolism; D000081007:Cymenes; D004305:Dose-Response Relationship, Drug; D005260:Female; D066298:In Vitro Techniques; D051660:Intermediate-Conductance Calcium-Activated Potassium Channels / Q000378:metabolism; D039821:Monoterpenes / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D050916:TRPV Cation Channels / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation; D055988:Uterine Artery / Q000187:drug effects / Q000378:metabolism*; D014664:Vasodilation / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D000081007:Cymenes; D051660:Intermediate-Conductance Calcium-Activated Potassium Channels; D039821:Monoterpenes; D012333:RNA, Messenger; D050916:TRPV Cation Channels; C505767:TRPV3 protein, rat; D014665:Vasodilator Agents; D009569:Nitric Oxide; C073316:carvacrol; D017869:Cyclic GMP-Dependent Protein Kinases
keywords: Carvacrol; Pregnancy; Radial artery; TRPV3; Uterus