title: Computational Prediction of Immunodominant Epitopes on Outer Membrane Protein (Omp) H of Pasteurella multocida Toward Designing of a Peptide Vaccine.
abstract: Contemporary vaccine design necessitates discrimination between the immunogenic and non-immunogenic components within a pathogen. To successfully target a humoral immune response, the vaccine antigen should contain not only B-cell epitopes but abounding Th-cell agretopes and MHC-II binding regions as well. No single computational method is available that allows the identification of such regions on antigens with good reliability. A consensus approach based on several prediction methods can be adopted to overcome this problem.Targeting the outer membrane protein (Omp) H as a candidate, a comprehensive work flow is described for the computational identification of immunodominant epitopes toward the designing of a peptide vaccine against Pasteurella multocida.
authors.affiliation: Animal Biotechnology Center, Department of Veterinary Physiology and Biochemistry, College of Veterinary and Animal Sciences, G. B. Pant University of Agriculture and Technology, Pantnagar, 263145, Uttarakhand, India. vetbhaskar@gmail.com.|, D-04, Alliance Kingston Estate, Rudrapur, 263153, Uttarakhand, India. vetbhaskar@gmail.com.
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Ganguly
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000276:immunology*; D001428:Bacterial Vaccines / Q000737:chemistry / Q000276:immunology*; D019295:Computational Biology / Q000379:methods*; D016056:Immunodominant Epitopes / Q000276:immunology*; D058751:Livestock / Q000276:immunology; D016979:Pasteurella multocida / Q000276:immunology*; D016415:Sequence Alignment
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D016056:Immunodominant Epitopes
keywords: B-cell epitope prediction; Immunoinformatics; Outer membrane protein H; Pasteurella multocida; Peptide vaccine; Th-cell agretope prediction