title: Characterization of the apelin -1860T>C polymorphism in Turkish coronary artery disease patients and healthy individuals.
abstract: To evaluate the association between the apelin -1860T>C polymorphism and plasma apelin levels in Turkish patients with coronary artery disease (CAD). A total of 276 individuals were enrolled in the present study, including 158 patients with CAD and 118 individuals without CAD as controls. The presence of the apelin -1860T>C gene polymorphism and plasma apelin levels were determined using polymerase chain reaction/restriction fragment length polymorphism and enzyme-linked immunosorbent assay, respectively. Significance was set at p≤0.05 for all statistical analyses. The genotype and allele frequencies of interested genes were significantly different between groups (χ(2)=10.2; df=2; p=0.006 and χ(2)=13.4; df=1; p=0.000, respectively). Frequency of CC genotype and the C allele of -1860T>C site was significantly higher in CAD patients compared to healthy controls. We found that individuals with the TC and CC genotypes were associated with an increased risk of CAD when compared with the TT genotype in CAD patients, and the adjusted ORs (95% CI) were 6.50 (1.27-33.0) and 6.39 (1.77-23.0), respectively. Plasma apelin levels were significantly lower in CAD patients compared to control group. Apelin level of CAD patient group having CC genotype of -1860T>C site was significantly lower compared to those having TT genotypes, but it was not statistically significant (p > 0.05). The homozygous CC genotype of apelin gene is associated with high risk of CAD. Apelin gene polymorphism -1860T>C is a significant predictor of predisposition to CAD in in Turkish population.
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Dumlupınar Kütahya, Turkey.
authors.forename: Raziye
authors.initials: R
authors.lastname: Akcılar
authors.affiliation: Department of Cardiovascular Surgery, Faculty of Medicine, University of Namık Kemal Tekirdağ, Turkey.
authors.forename: Gündüz
authors.initials: G
authors.lastname: Yümün
authors.affiliation: Department of Biochemistry, Faculty of Arts and Sciences, University of Dumlupınar Kütahya, Turkey.
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Bayat
authors.affiliation: Department of Cardiovascular Surgery, Faculty of Medicine, University of Namık Kemal Tekirdağ, Turkey.
authors.forename: Okan
authors.initials: O
authors.lastname: Donbaloğlu
authors.affiliation: Department of Cardiology, Faculty of Medicine, University of Namık Kemal Tekirdağ, Turkey.
authors.forename: Kubilay
authors.initials: K
authors.lastname: Erselcan
authors.affiliation: Department of Biology, Faculty of Arts and Sciences, University of Dumlupınar Kütahya, Turkey.
authors.forename: Ezgi
authors.initials: E
authors.lastname: Ece
authors.affiliation: Department of Biology, Faculty of Arts and Sciences, University of Dumlupınar Kütahya, Turkey.
authors.forename: Hülya
authors.initials: H
authors.lastname: Kökdaşgil
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Dumlupınar Kütahya, Turkey.
authors.forename: Osman
authors.initials: O
authors.lastname: Genç
keywords: Apelin -1860T>C gene; coronary artery disease; polymorphism