title: Formation of water-soluble soybean polysaccharides from spent flakes by hydrogen peroxide treatment.
abstract: In this paper we propose a novel chemical process for the generation of water-soluble polysaccharides from soy spent flake, a by-product of the soy food industry. This process entails treatment of spent flake with hydrogen peroxide at an elevated temperature, resulting in the release of more than 70% of the original insoluble material as high molar mass soluble polysaccharides. A design of experiment was used to quantify the effects of pH, reaction time, and hydrogen peroxide concentration on the reaction yield, average molar mass, and free monosaccharides generated. The resulting product is low in protein, fat, and minerals and contains predominantly water-soluble polysaccharides of high molar mass, including arabinan, type I arabinogalactan, homogalacturonan, xyloglucan, rhamnogalacturonan, and (glucurono)arabinoxylan. This treatment provides a straightforward approach for generation of soluble soy polysaccharides and opens a new range of opportunities for this abundant and underutilized material in future research and industrial applications.
authors.affiliation: DuPont™ Nutrition Biosciences ApS, Edwin Rahrs Vej 38, Brabrand 8220, Denmark; Department of Chemical and Biochemical Engineering, Center for Bioprocess Engineering, Technical University of Denmark, Søltofts Plads, Building 229, Kgs. Lyngby 2800, Denmark. Electronic address: brian.pierce@dupont.com.
authors.forename: Brian C
authors.initials: BC
authors.lastname: Pierce
authors.affiliation: DuPont™ Nutrition Biosciences ApS, Edwin Rahrs Vej 38, Brabrand 8220, Denmark. Electronic address: jesper.wichmann@dupont.com.
authors.forename: Jesper
authors.initials: J
authors.lastname: Wichmann
authors.affiliation: DuPont™ Protein Solutions, 4300 Duncan Avenue Saint Louis, MO 63110, USA. Electronic address: tam.h.tran@dupont.com.
authors.forename: Tam H
authors.initials: TH
authors.lastname: Tran
authors.affiliation: ARC Centre of Excellence in Plant Cell Walls, School of Biosciences, University of Melbourne, Melbourne VIC, 3010, Australia. Electronic address: roshanc@unimelb.edu.au.
authors.forename: Roshan
authors.initials: R
authors.lastname: Cheetamun
authors.affiliation: ARC Centre of Excellence in Plant Cell Walls, School of Biosciences, University of Melbourne, Melbourne VIC, 3010, Australia. Electronic address: abacic@unimelb.edu.au.
authors.forename: Antony
authors.initials: A
authors.lastname: Bacic
authors.affiliation: Department of Chemical and Biochemical Engineering, Center for Bioprocess Engineering, Technical University of Denmark, Søltofts Plads, Building 229, Kgs. Lyngby 2800, Denmark. Electronic address: am@kt.dtu.dk.
authors.forename: Anne S
authors.initials: AS
authors.lastname: Meyer
mesh_terms: D006861:Hydrogen Peroxide / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D012995:Solubility; D013025:Glycine max / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D014867:Water; D006861:Hydrogen Peroxide
keywords: Hydrogen peroxide; Soluble fiber; Soy polysaccharides; Spent flakes