title: Description of Salilacibacter albus gen. nov., sp. nov., isolated from a dried salt lake, and reclassification of Paraglycomyces xinjiangensis Luo et al. 2015 as a later heterotypic synonym of Salininema proteolyticum Nikou et al. 2015 with emended descriptions of the genus Salininema and Salininema proteolyticum.
abstract: A novel halophilic actinomycete, designated strain J11Y309T, was isolated from a soil sample collected from a dried salt lake in China. This isolate grew optimally at 28‒37 °C, with 3‒5 % (w/v) NaCl and at pH 7.0‒7.5. It contained meso-diaminopimelic acid as the diagnostic diamino acid and glucose, ribose and xylose were present in the whole-cell hydrolysates. MK-10(H4) was detected as the predominant menaquinone. The major fatty acids were anteiso-C17 : 0, iso-C16 : 0, iso-C17 : 0 and iso-C15 : 0. Polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, phosphoglycolipids, glycolipids, an unidentified phospholipid and additional unidentified lipids. The G+C content of the genomic DNA was 63.0 mol%. The novel strain shared highest 16S rRNA gene sequence similarity with Salininema proteolyticum Miq-4T(95.80 %), Paraglycomyces xinjiangensis TRM 49201T(95.77 %) and Haloglycomyces albus YIM 92370T(94.84 %). Phylogenetic trees showed that it could not be clearly assigned to any known genus within the family Glycomycetaceae and formed a distinct phylogenetic line in the clade comprising members of the genera Salininema, Paraglycomyces and Haloglycomyces. Based on data from the present polyphasic taxonomic study, strain J11Y309Trepresents a novel species of a new genus in the family Glycomycetaceae, for which the name Salilacibacter albus gen. nov., sp. nov. is proposed with Salilacibacter albus sp. nov. as the type species. The type strain of Salilacibacter albus is J11Y309T(=DSM 46875T=CGMCC 4.7242T=LMG 29297T). Reclassification of Paraglycomyces xinjiangensis Luo et al. 2015 as a later heterotypic synonym of Salininema roteolyticum Nikou et al. 2015 is also discussed in this study.
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.|College of Laboratory Medical Science, Hebei North University, Zhangjiakou 075000, PR China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Li
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
authors.forename: Jia-Meng
authors.initials: JM
authors.lastname: Liu
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.|Faculty of Basic Medical Sciences, Guilin Medical University, Guilin 541004, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.|Faculty of Basic Medical Sciences, Guilin Medical University, Guilin 541004, PR China.
authors.forename: Wei-Ming
authors.initials: WM
authors.lastname: Zhang
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
authors.forename: Shao-Wei
authors.initials: SW
authors.lastname: Liu
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.affiliation: Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Hu
authors.affiliation: Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: Faculty of Basic Medical Sciences, Guilin Medical University, Guilin 541004, PR China.
authors.forename: Da-Lin
authors.initials: DL
authors.lastname: Huang
authors.affiliation: Department of Chemistry, Johns Hopkins University, Baltimore, MD 21218, USA.
authors.forename: Rong-Feng
authors.initials: RF
authors.lastname: Li
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
authors.forename: Cheng-Hang
authors.initials: CH
authors.lastname: Sun
mesh_terms: D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D060106:Lakes / Q000382:microbiology*; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D003960:Diaminopimelic Acid
keywords: Glycomycetaceae; Paraglycomyces xinjiangensis; Salilacibacter albus; Salininema proteolyticum; halophilic actinobacterium; salt lake