title: Continuous biohydrogen production from waste bread by anaerobic sludge.
abstract: In this study, continuous biohydrogen production from waste bread by anaerobic sludge was performed. The waste bread was first hydrolyzed by the crude enzymes which were generated by Aspergillus awamori and Aspergillus oryzae via solid-state fermentation. It was observed that 49.78g/L glucose and 284.12mg/L free amino nitrogen could be produced with waste bread mass ratio of 15% (w/v). The waste bread hydrolysate was then used for biohydrogen production by anaerobic sludge in a continuous stirred tank reactor (CSTR). The optimal hydrogen production rate of 7.4L/(Ld) was achieved at chemical oxygen demand (COD) of 6000mg/L. According to the results obtained from this study, 1g waste bread could generate 0.332g glucose which could be further utilized to produce 109.5mL hydrogen. This is the first study which reports continuous biohydrogen production from waste bread by anaerobic sludge.
authors.affiliation: College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou 310018, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Han
authors.affiliation: College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou 310018, China.
authors.forename: Jingang
authors.initials: J
authors.lastname: Huang
authors.affiliation: College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou 310018, China.
authors.forename: Hongting
authors.initials: H
authors.lastname: Zhao
authors.affiliation: School of Forestry, Northeast Forestry University, Harbin 150060, China. Electronic address: dr_lyf@nefu.edu.cn.
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Li
mesh_terms: D000693:Anaerobiosis; D001230:Aspergillus / Q000201:enzymology; D056804:Biofuels; D019149:Bioreactors; D001939:Bread; D005285:Fermentation; D006859:Hydrogen / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D012722:Sewage; D018505:Waste Management / Q000379:methods
chemical_list: D056804:Biofuels; D012722:Sewage; D006859:Hydrogen
keywords: Biohydrogen production; Chemical oxygen demand; Continuous stirred tank reactor; Waste bread