title: Glucose-6-phosphate dehydrogenase plays a central role in the response of tomato (Solanum lycopersicum) plants to short and long-term drought.
abstract: The present study was undertaken to investigate the expression, occurrence and activity of glucose 6 phosphate dehydrogenase (G6PDH - EC 1.1.1.49), the key-enzyme of the Oxidative Pentose Phosphate Pathway (OPPP), in tomato plants (Solanum lycopersicum cv. Red Setter) exposed to short- and long-term drought stress. For the first time, drought effects have been evaluated in plants under different growth conditions: in hydroponic laboratory system, and in greenhouse pots under controlled conditions; and in open field, in order to evaluate drought response in a representative agricultural environment. Interestingly, changes observed appear strictly associated to the induction of well known stress response mechanisms, such as the increase of proline synthesis, accumulation of chaperone Hsp70, and ascorbate peroxidase. Results show significant increase in total activity of G6PDH, and specifically in expression and occurrence of cytosolic isoform (cy-G6PDH) in plants grown in any cultivation system upon drought. Intriguingly, the results clearly suggest that abscissic acid (ABA) pathway and signaling cascade (protein phosphatase 2C PP2C) could be strictly related to increased G6PDH expression, occurrence and activities. We hypothesized for G6PDH a specific role as one of the main reductants' suppliers to counteract the effects of drought stress, in the light of converging evidences given by young and adult tomato plants under stress of different duration and intensity.
authors.affiliation: Università di Napoli ''Federico II", Dipartimento di Biologia, Via Cinthia, I-80126, Napoli, Italy; National Research Council of Italy, Institute of Biosciences and Bioresources, Research Division Portici, Portici, 80055, Naples, Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Landi
authors.affiliation: National Research Council of Italy, Institute of Biosciences and Bioresources, Research Division Portici, Portici, 80055, Naples, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Nurcato
authors.affiliation: Università di Napoli ''Federico II", Dipartimento di Biologia, Via Cinthia, I-80126, Napoli, Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: De Lillo
authors.affiliation: Università di Napoli ''Federico II", Dipartimento di Biologia, Via Cinthia, I-80126, Napoli, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Lentini
authors.affiliation: National Research Council of Italy, Institute of Biosciences and Bioresources, Research Division Portici, Portici, 80055, Naples, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Grillo
authors.affiliation: Università di Napoli ''Federico II", Dipartimento di Biologia, Via Cinthia, I-80126, Napoli, Italy. Electronic address: sergio.esposito@unina.it.
authors.forename: Sergio
authors.initials: S
authors.lastname: Esposito
mesh_terms: D003681:Dehydration; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D018551:Solanum lycopersicum / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D054046:Plant Stomata / Q000502:physiology; D013312:Stress, Physiological; D013997:Time Factors
chemical_list: D007527:Isoenzymes; D010940:Plant Proteins; D005954:Glucosephosphate Dehydrogenase
keywords: ABA; Abiotic stress; Drought; G6PDH; NCED; Oxidative stress; P5CS; Tomato