title: HD2C histone deacetylase and a SWI/SNF chromatin remodelling complex interact and both are involved in mediating the heat stress response in Arabidopsis.
abstract: Studies in yeast and animals have revealed that histone deacetylases (HDACs) often act as components of multiprotein complexes, including chromatin remodelling complexes (CRCs). However, interactions between HDACs and CRCs in plants have yet to be demonstrated. Here, we present evidence for the interaction between Arabidopsis HD2C deacetylase and a BRM-containing SWI/SNF CRC. Moreover, we reveal a novel function of HD2C as a regulator of the heat stress response. HD2C transcript levels were strongly induced in plants subjected to heat treatment, and the expression of selected heat-responsive genes was up-regulated in heat-stressed hd2c mutant, suggesting that HD2C acts to down-regulate heat-activated genes. In keeping with the HDAC activity of HD2C, the altered expression of HD2C-regulated genes coincided in most cases with increased histone acetylation at their loci. Microarray transcriptome analysis of hd2c and brm mutants identified a subset of commonly regulated heat-responsive genes, and the effect of the brm hd2c double mutation on the expression of these genes was non-additive. Moreover, heat-treated 3-week-old hd2c, brm and brm hd2c mutants displayed similar rates of growth retardation. Taken together, our findings suggest that HD2C and BRM act in a common genetic pathway to regulate the Arabidopsis heat stress response.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106, Warsaw, Poland. danielb@ibb.waw.pl.
authors.forename: Daniel
authors.initials: D
authors.lastname: Buszewicz
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106, Warsaw, Poland.|Laboratory of Systems Biology, Faculty of Biology, University of Warsaw, 02-106, Warsaw, Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Archacki
authors.affiliation: Laboratory of Systems Biology, Faculty of Biology, University of Warsaw, 02-106, Warsaw, Poland.
authors.forename: Antoni
authors.initials: A
authors.lastname: Palusiński
authors.affiliation: Laboratory of Systems Biology, Faculty of Biology, University of Warsaw, 02-106, Warsaw, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Kotliński
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106, Warsaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Fogtman
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106, Warsaw, Poland.|Laboratory of Systems Biology, Faculty of Biology, University of Warsaw, 02-106, Warsaw, Poland.
authors.forename: Roksana
authors.initials: R
authors.lastname: Iwanicka-Nowicka
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106, Warsaw, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Sosnowska
authors.affiliation: Laboratory of Systems Biology, Faculty of Biology, University of Warsaw, 02-106, Warsaw, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Kuciński
authors.affiliation: Department of Plant Physiology, Genetics and Biotechnology, University of Warmia and Mazury in Olsztyn, 10-719, Olsztyn, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Pupel
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106, Warsaw, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Olędzki
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106, Warsaw, Poland.|Institute of Genetics and Biotechnology, University of Warsaw, 02-106, Warsaw, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Dadlez
authors.affiliation: Department of Chemistry, Biological and Chemical Research Centre, University of Warsaw, 00-927, Warsaw, Poland.|Mossakowski Medical Research Centre, Polish Academy of Sciences, 02-106, Warsaw, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Misicka
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106, Warsaw, Poland.|Laboratory of Systems Biology, Faculty of Biology, University of Warsaw, 02-106, Warsaw, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Jerzmanowski
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106, Warsaw, Poland. marta@ibb.waw.pl.|Laboratory of Systems Biology, Faculty of Biology, University of Warsaw, 02-106, Warsaw, Poland. marta@ibb.waw.pl.
authors.forename: Marta Kamila
authors.initials: MK
authors.lastname: Koblowska
mesh_terms: D000107:Acetylation; D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D017360:Arabidopsis / Q000201:enzymology / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D042002:Chromatin Assembly and Disassembly; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018869:Heat-Shock Response; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D006657:Histones / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology
chemical_list: C463463:ATSWI3B protein, Arabidopsis; D029681:Arabidopsis Proteins; D006657:Histones; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C510447:HD2C protein, Arabidopsis; D006655:Histone Deacetylases; D000251:Adenosine Triphosphatases; C514541:BRM protein, Arabidopsis
keywords: BRM; SWI3B; histone acetylation; transcriptome