title: Is Centrifugation Necessary for Processing Lipoaspirate Harvested via Water-Jet Force Assisted Technique before Grafting? Evidence of Lipoaspirate Concentration With Enhanced Fat Graft Survival.
abstract: BACKGROUND
  Although water-jet force-assisted liposuction technique (WAL) was demonstrated to have favorable effects on fat grafting, controversy continues concerning the application of centrifugation for lipoaspirate harvested via WAL. As a controversial technique, plastic surgeons often get perplexed to the necessity of using centrifugation during fat grafting procedure. In the present study, we adopted the recommended centrifugal intensity (1200g, 3 minutes) to process lipoaspirate and focused on the influence of centrifugation on the fate of lipoaspirate harvested with WAL technique.
  
  
  METHODS
  Lipoaspirate was obtained from 10 healthy Chinese female patients who underwent cosmetic liposuction. The harvested lipoaspirate was either not centrifuged (group A) or centrifuged at 1200g for 3 minutes (group B). Lipoaspirate from each group was compared in the in vitro and in vivo experiments. The influence of centrifugation on lipoaspirate viability and lipoaspirate survival after grafting were evaluated.
  
  
  RESULTS
  The viability of the lipoaspirates was similar between equally volumetric uncentrifugal and centrifugal lipoaspirate. However, centrifugation at 1200g for 3 minutes concentrated stromal vascular fraction cells and adipose-derived stem cells in lipoaspirate; greater angiogenesis and weight retention rates were observed in centrifugal lipoaspirate after grafting than those uncentrifugal lipoaspirate.
  
  
  CONCLUSIONS
  Centrifugation at 1200g for 3 minutes enhanced the survival of lipoaspirate harvested via WAL technique after grafting. Centrifugation at 1200g for 3 minutes was recommended to process lipoaspirate harvested with water-jet force assistance before grafting.
authors.affiliation: From the *Breast Plastic and Reconstructive Surgery Center, and †Department of Pathology, Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, People's Republic of China.
authors.forename: Shilu
authors.initials: S
authors.lastname: Yin
authors.forename: Jie
authors.initials: J
authors.lastname: Luan
authors.forename: Su
authors.initials: S
authors.lastname: Fu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhuang
mesh_terms: D000328:Adult; D000818:Animals; D002498:Centrifugation; D005260:Female; D006085:Graft Survival; D006801:Humans; D015187:Lipectomy / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation; D019651:Plastic Surgery Procedures / Q000379:methods*; D016037:Single-Blind Method; D050151:Subcutaneous Fat / Q000637:transplantation*