title: [Study on interference effect of Sijunzi decoction on brain-gut CaM/CaMK II of spleen Qi deficiency syndrome rats].
abstract: OBJECTIVE
  To observe the dynamic time-phase expressions of key genes of brain-gut CaM signal pathway of spleen Qi deficiency rats and the intervention effect of Sijunzi decoction.
  
  
  METHODS
  Male Wistar rats were randomly divided into the normal control group, model 14 d, 21 d, 28 d groups, and Sijunzi decoction 14 d, 21 d, 28 d groups. Except for the normal control group, the remaining groups were included into the spleen Qi deficiency model with the bitter cold breaking Qi method (ig 7.5 g · kg⁻¹ · d⁻¹ of Rheum officinale, Fructus aurantii immaturus, Magnolia officinalis preparation) and the exhaustive swimming method. On the 7th day after the modeling, the Sijunzi decoction groups were orally administered with Sijunzi decoction 20 g · kg⁻¹ · d⁻¹. The expressions of key genes CaM/CaMK II of CaM signaling pathway in hippocampus and intestine at different time points by immunohistochemical method and Western blot. At the same time, the intervention effect of Sijunzi decoction on spleen Qi deficiency rats and its mechanism were analyzed.
  
  
  RESULTS
  Spleen Qi deficiency rats showed higher intestinal CaM/CaMK II expression and lower hippocampus CaM/CaMK II expression than normal rats (P < 0.05, P < 0.01). After the treatment of Sijunzi decoction, spleen Qi deficiency rats showed reduction in intestinal CaM/CaMK II expression and increase in hippocampus CaM/CaMK II expression (P < 0.05, P < 0.01).
  
  
  CONCLUSIONS
  The formation of spleen Qi deficiency syndrome may be related to the high expression of CaM/CaMK II in small intestine tissues and its low expression in hippocampus tissues. Sijunzi decoction may achieve the therapeutic effect in spleen Qi deficiency syndrome by reducing the CaM/CaMK II expression in intestinal tissues and increasing it in hippocampus tissues.
authors.forename: Rong
authors.initials: R
authors.lastname: Tian
authors.forename: Zi-han
authors.initials: ZH
authors.lastname: Gong
authors.forename: Xiao-yi
authors.initials: XY
authors.lastname: Yang
authors.forename: Li-ming
authors.initials: LM
authors.lastname: Zhu
authors.forename: Yong-qiang
authors.initials: YQ
authors.lastname: Duan
authors.forename: Ying-xia
authors.initials: YX
authors.lastname: Cheng
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000235:genetics / Q000378:metabolism*; D002147:Calmodulin / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D019413:Qi; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000187:drug effects*; D013158:Splenic Diseases / Q000188:drug therapy* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism
chemical_list: D002147:Calmodulin; D004365:Drugs, Chinese Herbal; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2