title: Antioxidant effects of cultured wild ginseng root extracts on the male reproductive function of boars and guinea pigs.
abstract: The main objective of this study was to investigate the effects of cultured wild ginseng root extracts (cWGRE) on the sperm of boars and the reproductive system of guinea pigs. Firstly, semen collected from boars (n=10) were incubated in 38°C for 1h with xanthine and xanthine oxidase to generate ROS. The cWGRE was added to the sperm culture system to test its antioxidant effect on the boar sperm. The amount of Reactive Oxygen Species (ROS) was measured by a chemiluminescence assay using luminol. The results indicated that the addition of cWGRE to boar sperm culture inhibited xanthine and xanthine oxidase-induced ROS concentrations. Treatment with cWGRE also had a positive effect on maintaining sperm motility. Effects of cWGRE administration on vitamin C-deficient guinea pigs were further investigated. Hartley guinea pigs (n=25) at 8 weeks of age were randomly divided into five groups. With the exception of the positive control group, each group was fed vitamin C-deficient feed for 21days (d). Respective groups were also orally administered cWGRE, ginseng extract, or mixed ginsenosides for 21 days. In comparison to the control group, oral administration of cWGRE reduced (P<0.05) amount of lipid peroxidation and increased (P<0.05) both glutathione peroxidase concentrations and the trolox equivalent antioxidant capacity. In addition, administration of cWGRE induced increases (P<0.05) in body weight, testosterone concentrations, and spermatid populations. The results of the present study support our hypothesis that cWGRE has positive effects on male reproductive functions via suppression of ROS production.
authors.affiliation: Department of Animal Science & Technology, Chung-Ang University, Anseong 17546, Republic of Korea.
authors.forename: Suk Jun
authors.initials: SJ
authors.lastname: Yun
authors.affiliation: Department of Animal Science & Technology, Chung-Ang University, Anseong 17546, Republic of Korea.
authors.forename: Gui-Seck
authors.initials: GS
authors.lastname: Bae
authors.affiliation: Department of Animal Science & Technology, Chung-Ang University, Anseong 17546, Republic of Korea.
authors.forename: Jae Hawn
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Animal Science & Technology, Chung-Ang University, Anseong 17546, Republic of Korea.
authors.forename: Tae Ho
authors.initials: TH
authors.lastname: Song
authors.affiliation: Department of Animal Science & Technology, Chung-Ang University, Anseong 17546, Republic of Korea.
authors.forename: Ahreum
authors.initials: A
authors.lastname: Choi
authors.affiliation: Department of Animal Science & Technology, Chung-Ang University, Anseong 17546, Republic of Korea.
authors.forename: Buom-Yong
authors.initials: BY
authors.lastname: Ryu
authors.affiliation: Department of Animal Science & Technology, Chung-Ang University, Anseong 17546, Republic of Korea.
authors.forename: Myung-Geol
authors.initials: MG
authors.lastname: Pang
authors.affiliation: Department of Animal Science, Kyungpook National University, Sangju 37224, Republic of Korea.
authors.forename: Eun Joong
authors.initials: EJ
authors.lastname: Kim
authors.affiliation: Department of Horse, Companion, and Wild Animal Science, Kyungpook National University, Sangju 37224, Republic of Korea. Electronic address: mjyoon@knu.ac.kr.
authors.forename: Minjung
authors.initials: M
authors.lastname: Yoon
authors.affiliation: Department of Animal Science & Technology, Chung-Ang University, Anseong 17546, Republic of Korea. Electronic address: moonbaek@cau.ac.kr.
authors.forename: Moon Baek
authors.initials: MB
authors.lastname: Chang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D006168:Guinea Pigs; D015227:Lipid Peroxidation; D008297:Male; D005894:Panax / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D013081:Sperm Motility / Q000187:drug effects; D013087:Spermatids; D013091:Spermatogenesis; D013094:Spermatozoa / Q000502:physiology; D013552:Swine / Q000502:physiology*; D013739:Testosterone / Q000097:blood
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D013739:Testosterone; D005978:Glutathione
keywords: Antioxidant; Boars; Cultured wild ginseng root extracts (cWGRE); Guinea pigs; Reactive oxygen species (ROS); Sperm