title: ISOLATION AND GENOTYPING OF Toxoplasma gondii IN SERONEGATIVE URBAN RATS AND PRESENCE OF ANTIBODIES IN COMMUNICATING DOGS IN BRAZIL.
abstract: The role of rodents in the epidemiology of toxoplasmosis was investigated in Londrina, Paraná State, Brazil. One hundred and eighty-one Rattus rattus and one Mus musculus were caught in 37 places. Blood and tissues were collected and submitted to the indirect fluorescence antibody test (IFAT) and the bioassay. Serum samples from 61 contacting dogs were also collected. Sixteen rats (8.8%) were positive for Toxoplasma gondii, but just two of them were positive by serology and bioassay test. Antibodies were found in nine (4.9%) rats. Tissues of nine rats bioassayed were positive and four isolates were obtained. Restriction fragment length polymorphism (RFLP) analysis was performed using 12 markers (SAG1, SAG2, SAG2-alt, C22-8, C29-2, L358, PK1, BTUB, GRA6, SAG3, Apico, CS3). Genotyping revealed that the four strains isolated from this study have been isolated before in cats and chickens from Brazil. None of the isolates was identified like clonal archetypal T-types I, II, and III. The rats presented lower serologic Toxoplasma gondii prevalence (8.8%) compared to contacting dogs (70.5%).
authors.affiliation: Programa de pós-graduação em Ciência Animal, Universidade Estadual de Londrina, Londrina, PR, Brasil, brunoruffolo@ig.com.br, betatoledo@yahoo.com.br, felippemartins@hotmail.com, bugni@pop.com.br, lelepaty2@yahoo.com.br.
authors.forename: Bruno Bergamo
authors.initials: BB
authors.lastname: Ruffolo
authors.affiliation: Programa de pós-graduação em Ciência Animal, Universidade Estadual de Londrina, Londrina, PR, Brasil, brunoruffolo@ig.com.br, betatoledo@yahoo.com.br, felippemartins@hotmail.com, bugni@pop.com.br, lelepaty2@yahoo.com.br.
authors.forename: Roberta dos Santos
authors.initials: Rdos S
authors.lastname: Toledo
authors.affiliation: Programa de pós-graduação em Ciência Animal, Universidade Estadual de Londrina, Londrina, PR, Brasil, brunoruffolo@ig.com.br, betatoledo@yahoo.com.br, felippemartins@hotmail.com, bugni@pop.com.br, lelepaty2@yahoo.com.br.
authors.forename: Felippe Danyel Cardoso
authors.initials: FD
authors.lastname: Martins
authors.affiliation: Programa de pós-graduação em Ciência Animal, Universidade Estadual de Londrina, Londrina, PR, Brasil, brunoruffolo@ig.com.br, betatoledo@yahoo.com.br, felippemartins@hotmail.com, bugni@pop.com.br, lelepaty2@yahoo.com.br.
authors.forename: Felipe Monteiro
authors.initials: FM
authors.lastname: Bugni
authors.affiliation: Programa de pós-graduação em Ciência Animal, Universidade Estadual de Londrina, Londrina, PR, Brasil, brunoruffolo@ig.com.br, betatoledo@yahoo.com.br, felippemartins@hotmail.com, bugni@pop.com.br, lelepaty2@yahoo.com.br.
authors.forename: Letícia da
authors.initials: Ld
authors.lastname: Costa
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Centro de Ciências Agrárias, Universidade Estadual de Londrina, Londrina, PR, Brasil, elizabetemarana@yahoo.com.br, italmar@uel.br, jlgarcia@uel.br, rlfreire@uel.br.
authors.forename: Elizabete Regina Marangoni
authors.initials: ER
authors.lastname: Marana
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Centro de Ciências Agrárias, Universidade Estadual de Londrina, Londrina, PR, Brasil, elizabetemarana@yahoo.com.br, italmar@uel.br, jlgarcia@uel.br, rlfreire@uel.br.
authors.forename: Italmar Teodorico
authors.initials: IT
authors.lastname: Navarro
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Centro de Ciências Agrárias, Universidade Estadual de Londrina, Londrina, PR, Brasil, elizabetemarana@yahoo.com.br, italmar@uel.br, jlgarcia@uel.br, rlfreire@uel.br.
authors.forename: João Luis
authors.initials: JL
authors.lastname: Garcia
authors.affiliation: Department of Microbiology, University of Tennessee, Knoxville, TN, United States, csu1@utk.edu.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Su
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Centro de Ciências Agrárias, Universidade Estadual de Londrina, Londrina, PR, Brasil, elizabetemarana@yahoo.com.br, italmar@uel.br, jlgarcia@uel.br, rlfreire@uel.br.
authors.forename: Roberta Lemos
authors.initials: RL
authors.lastname: Freire
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D001938:Brazil / Q000453:epidemiology; D002415:Cats; D004283:Dog Diseases / Q000453:epidemiology / Q000469:parasitology*; D004285:Dogs; D019084:Fluorescent Antibody Technique, Indirect; D005838:Genotype; D051379:Mice; D012150:Polymorphism, Restriction Fragment Length; D051381:Rats / Q000469:parasitology*; D014122:Toxoplasma / Q000276:immunology / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000453:epidemiology / Q000469:parasitology*
chemical_list: D000913:Antibodies, Protozoan