title: Dopamine-assisted deposition of poly (ethylene imine) for efficient heparinization.
abstract: Mussel-inspired self-polymerization of dopamine in the presence of catechols and amines can be used to form coatings on various substrates. In this work dopamine was co-polymerized with poly (ethylene imine) (PEI) to form coatings on 316L stainless steel (SS). The coating formation was confirmed by Fourier transform infrared spectra (FTIR) and X-ray photoelectron spectroscopy (XPS). Compared with a pure polydopamine coating, such hybrid coating presented a higher ratio of nitrogen and increased zeta potential due to the introduction of PEI components. QCM measurement showed good affinity for heparin immobilization on the amine-rich surfaces. Moreover, the heparin-binding surface significantly suppressed platelet adhesion and activation. Further, the interaction with vascular cells, like endothelial cells (ECs) and smooth muscle cells (SMCs) was also investigated. The heparin coating not only decreased the cytotoxicity of amines on ECs, but also significantly inhibited SMCs proliferation. These results showed that the hybrid coating allowed effective heparin immobilization, providing a promising platform for vascular devices modification.
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China; Key Lab of Advanced Technology of Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Rifang
authors.initials: R
authors.lastname: Luo
authors.affiliation: Key Lab of Advanced Technology of Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Lab of Advanced Technology of Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Jinchuan
authors.initials: J
authors.lastname: Deng
authors.affiliation: Key Lab of Advanced Technology of Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Lab of Advanced Technology of Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China; Max Bergmann Center of Biomaterials Dresden, Leibniz Institute of Polymer Research Dresden, Hohe Strasse 6, 01069 Dresden, Germany.
authors.forename: Manfred F
authors.initials: MF
authors.lastname: Maitz
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: Key Lab of Advanced Technology of Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China. Electronic address: jinxxwang@263.net.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Lab of Advanced Technology of Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Huang
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China. Electronic address: yunbing.wang@scu.edu.cn.
authors.forename: Yunbing
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000588:Amines / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002450:Cell Communication / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004298:Dopamine / Q000737:chemistry*; D006493:Heparin / Q000737:chemistry* / Q000494:pharmacology; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D007097:Imines / Q000737:chemistry*; D008422:Materials Testing; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects; D011095:Polyethylenes / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D000588:Amines; D007097:Imines; D011095:Polyethylenes; C505405:poly(ethylene imine); D014867:Water; D006493:Heparin; D004298:Dopamine; D012825:Silicon
keywords: Dopamine; Endothelial cells; Heparin; Poly (ethylene imine); Smooth muscle cells; Surface modification