title: Hyaluronic Acid-Modified Cationic Lipid-PLGA Hybrid Nanoparticles as a Nanovaccine Induce Robust Humoral and Cellular Immune Responses.
abstract: Here, we investigated the use of hyaluronic acid (HA)-decorated cationic lipid-poly(lactide-co-glycolide) acid (PLGA) hybrid nanoparticles (HA-DOTAP-PLGA NPs) as vaccine delivery vehicles, which were originally developed for the cytosolic delivery of genes. Our results demonstrated that after the NPs uptake by dendritic cells (DCs), some of the antigens that were encapsulated in HA-DOTAP-PLGA NPs escaped to the cytosolic compartment, and whereas some of the antigens remained in the endosomal/lysosomal compartment, where both MHC-I and MHC-II antigen presentation occurred. Moreover, HA-DOTAP-PLGA NPs led to the up-regulation of MHC, costimulatory molecules, and cytokines. In vivo experiments further revealed that more powerful immune responses were induced from mice immunized with HA-DOTAP-PLGA NPs when compared with cationic lipid-PLGA nanoparticles and free ovalbumin (OVA); the responses included antigen-specific CD4(+) and CD8(+) T-cell responses, the production of antigen-specific IgG antibodies and the generation of memory CD4(+) and CD8(+) T cells. Overall, these data demonstrate the high potential of HA-DOTAP-PLGA NPs for use as vaccine delivery vehicles to elevate cellular and humoral immune responses.
authors.affiliation: The Tianjin Key Laboratory of Biomaterials, Institute of Biomedical Engineering, Peking Union Medical College & Chinese Academy of Medical Sciences , Tianjin 300192, China.
authors.forename: Lanxia
authors.initials: L
authors.lastname: Liu
authors.affiliation: The Tianjin Key Laboratory of Biomaterials, Institute of Biomedical Engineering, Peking Union Medical College & Chinese Academy of Medical Sciences , Tianjin 300192, China.
authors.forename: Fengqiang
authors.initials: F
authors.lastname: Cao
authors.affiliation: The Tianjin Key Laboratory of Biomaterials, Institute of Biomedical Engineering, Peking Union Medical College & Chinese Academy of Medical Sciences , Tianjin 300192, China.
authors.forename: Xiaoxuan
authors.initials: X
authors.lastname: Liu
authors.affiliation: The Tianjin Key Laboratory of Biomaterials, Institute of Biomedical Engineering, Peking Union Medical College & Chinese Academy of Medical Sciences , Tianjin 300192, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Wang
authors.affiliation: The Tianjin Key Laboratory of Biomaterials, Institute of Biomedical Engineering, Peking Union Medical College & Chinese Academy of Medical Sciences , Tianjin 300192, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: The Tianjin Key Laboratory of Biomaterials, Institute of Biomedical Engineering, Peking Union Medical College & Chinese Academy of Medical Sciences , Tianjin 300192, China.
authors.forename: Hongfan
authors.initials: H
authors.lastname: Sun
authors.affiliation: The Tianjin Key Laboratory of Biomaterials, Institute of Biomedical Engineering, Peking Union Medical College & Chinese Academy of Medical Sciences , Tianjin 300192, China.|Department of Biomedical Engineering, University of Minnesota , Minneapolis, Minnesota 55455, United States.
authors.forename: Chun
authors.initials: C
authors.lastname: Wang
authors.affiliation: The Tianjin Key Laboratory of Biomaterials, Institute of Biomedical Engineering, Peking Union Medical College & Chinese Academy of Medical Sciences , Tianjin 300192, China.
authors.forename: Xigang
authors.initials: X
authors.lastname: Leng
authors.affiliation: The Tianjin Key Laboratory of Biomaterials, Institute of Biomedical Engineering, Peking Union Medical College & Chinese Academy of Medical Sciences , Tianjin 300192, China.
authors.forename: Cunxian
authors.initials: C
authors.lastname: Song
authors.affiliation: The Tianjin Key Laboratory of Biomaterials, Institute of Biomedical Engineering, Peking Union Medical College & Chinese Academy of Medical Sciences , Tianjin 300192, China.
authors.forename: Deling
authors.initials: D
authors.lastname: Kong
authors.affiliation: The Tianjin Key Laboratory of Biomaterials, Institute of Biomedical Engineering, Peking Union Medical College & Chinese Academy of Medical Sciences , Tianjin 300192, China.
authors.forename: Guilei
authors.initials: G
authors.lastname: Ma
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D016503:Drug Delivery Systems; D005260:Female; D006820:Hyaluronic Acid / Q000737:chemistry / Q000494:pharmacology; D007111:Immunity, Cellular / Q000187:drug effects*; D056724:Immunity, Humoral / Q000187:drug effects*; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry / Q000627:therapeutic use; D011100:Polyglycolic Acid / Q000737:chemistry / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D014612:Vaccines / Q000737:chemistry / Q000494:pharmacology
chemical_list: D014612:Vaccines; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D006820:Hyaluronic Acid
keywords: adjuvant; cationic lipid−PLGA hybrid nanoparticles; hyaluronic acid; immune response; vaccine