title: Design and Construction of Shrimp Antiviral DNA Vaccines Expressing Long and Short Hairpins for Protection by RNA Interference.
abstract: DNA vaccines present the aquaculture industry with an effective and economically viable method of controlling viral pathogens that drastically affect productivity. Since specific immune response is rudimentary in invertebrates, the presence of RNA interference (RNAi) pathway in shrimps provides a promising new approach to vaccination. Plasmid DNA vaccines that express short or long double stranded RNA in vivo have shown protection against viral diseases. The design, construction and considerations for preparing such vaccines are discussed.
authors.affiliation: ICAR-Central Institute of Fisheries Education, Versova, Andheri West, Mumbai, 400061, India. achaudhari67@gmail.com.
authors.forename: Aparna
authors.initials: A
authors.lastname: Chaudhari
authors.affiliation: ICAR-Central Institute of Fisheries Education, Versova, Andheri West, Mumbai, 400061, India.
authors.forename: Gireesh-Babu
authors.initials: GB
authors.lastname: Pathakota
authors.affiliation: ICAR-Central Institute of Fisheries Education, Versova, Andheri West, Mumbai, 400061, India.
authors.forename: Pavan-Kumar
authors.initials: PK
authors.lastname: Annam
mesh_terms: D000818:Animals; D001483:Base Sequence; D033364:Decapoda / Q000821:virology*; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D010957:Plasmids / Q000235:genetics; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D019444:Vaccines, DNA / Q000235:genetics* / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics* / Q000276:immunology
chemical_list: D034741:RNA, Small Interfering; D019444:Vaccines, DNA; D014765:Viral Vaccines
keywords: DNA vaccination; Double-stranded RNA; Long-hairpin RNA; Short-hairpin RNA; Shrimp; Vaccine