title: miR-144 suppresses the growth and metastasis of laryngeal squamous cell carcinoma by targeting IRS1.
abstract: Increasing evidence has been suggested that microRNA-144 (miR-144) involved in tumor initiation, development and metastasis in various cancers. However, the biological roles and potential mechanisms of miR-144 in laryngeal squamous cell carcinoma (LSCC) remain unclear. In the present study, we discovered that miR-144 expression levels in LSCC tissues were significantly lower than those of adjacent normal tissues. Furthermore, overexpression of miR-144 in LSCC cells inhibited cell proliferation, colony formation, migration, and invasion in vitro. Consistently, stable overexpression of miR-144 suppressed the growth of LSCC cell xenografts in vivo. Bioinformatic algorithms and luciferase reporter assays confirmed that insulin receptor substrate 1 (IRS1) is a direct target of miR-144. Overexpreesion of miR-144 obviously decreased IRS1 expression thereby suppressing phosphatidylinositol 3-kinase (PI3K)/AKT pathway activation. Further functional studies suggested that downregulation of IRS1 had similar effects as that of miR-144 overexpression, and upregulation of IRS1 partially reversed the inhibitory effects of miR-144. These findings suggested that miR-144 functioned as a tumor suppressor in LSCC by targeting IRS1, and that miR-144 might serve as a potential target for LSCC treatment.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, The First Hospital of Jilin University 3302 Jilin Main Road, Changchun 130021, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Anesthesiology, The First Hospital of Jilin University 3302 Jilin Main Road, Changchun 130021, China.
authors.forename: Chang-Lei
authors.initials: CL
authors.lastname: Cui
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, The First Hospital of Jilin University 3302 Jilin Main Road, Changchun 130021, China.
authors.forename: Wei-Lun
authors.initials: WL
authors.lastname: Chen
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, The First Hospital of Jilin University 3302 Jilin Main Road, Changchun 130021, China.
authors.forename: Zhong-Ying
authors.initials: ZY
authors.lastname: Fu
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, The First Hospital of Jilin University 3302 Jilin Main Road, Changchun 130021, China.
authors.forename: Xiang-Yan
authors.initials: XY
authors.lastname: Cui
authors.affiliation: Department of Hand Surgery, The First Hospital of Jilin University 3302 Jilin Main Road, Changchun 130021, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Gong
keywords: IRS1; growth; laryngeal squamous cell carcinoma; metastasis; miR-144