title: Electrophysiological Measurement of Cannabinoid-Mediated Synaptic Modulation in Acute Mouse Brain Slices.
abstract: Endocannabinoids (eCBs) are a class of bioactive lipids that mediate retrograde synaptic modulation at central and peripheral synapses. The highly lipophilic nature of eCBs and the pharmacological tools available to interrogate this system require unique methodological consideration, especially when applied to ex vivo systems such as electrophysiological analysis in acute brain slices. This unit provides protocols for measuring cannabinoid and eCB-mediated synaptic signaling in mouse brain slices, including analysis of short-term, long-term, and tonic eCB signaling modes, and the unique considerations for working with eCBs and TRPV1/cannabinoid ligands in acute brain slices.
authors.affiliation: Department of Psychiatry, Vanderbilt University Medical Center, Nashville, Tennessee.
authors.forename: Rita
authors.initials: R
authors.lastname: Báldi
authors.affiliation: Department of Anesthesiology, Vanderbilt University Medical Center, Nashville, Tennessee.
authors.forename: Dipanwita
authors.initials: D
authors.lastname: Ghosh
authors.affiliation: Department of Anesthesiology, Vanderbilt University Medical Center, Nashville, Tennessee.
authors.forename: Brad A
authors.initials: BA
authors.lastname: Grueter
authors.affiliation: Department of Psychiatry, Vanderbilt University Medical Center, Nashville, Tennessee.|Department of Molecular Physiology & Biophysics, Vanderbilt University Medical Center, Nashville, Tennessee.
authors.forename: Sachin
authors.initials: S
authors.lastname: Patel
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000502:physiology*; D063385:Cannabinoid Receptor Modulators / Q000494:pharmacology*; D063388:Endocannabinoids / Q000378:metabolism*; D066298:In Vitro Techniques; D051379:Mice; D009473:Neuronal Plasticity / Q000187:drug effects*; D018408:Patch-Clamp Techniques; D013569:Synapses / Q000187:drug effects*; D009435:Synaptic Transmission / Q000187:drug effects*
chemical_list: D063385:Cannabinoid Receptor Modulators; D063388:Endocannabinoids
keywords: CB1 receptor; cannabinoid; electrophysiology; endocannabinoid; mouse