title: Biotransformation of gypenoside XVII to compound K by a recombinant β-glucosidase.
abstract: OBJECTIVE
  To study the β-glucosidase gene (bgy1) from Lactobacillus brevis that was cloned and expressed in Escherichia coli BL21 (DE3) and then using it for the biotransformation of gypenoside XVII.
  
  
  RESULTS
  The bgy1 gene consists of 2283 bp encoding 761 amino acids, with homology to the glycosyl hydrolase family-3 protein domain. The enzyme (Bgy1) hydrolyzed the glucose moieties at the C-3 position and the outer glucose moieties at the C-20 position of gypenoside XVII. Using 0.1 mg enzyme ml(-1) in 20 mM sodium phosphate buffer at 30 °C and pH 6.0, 1 mg gypenoside XVII ml(-1) was transformed into 0.58 mg compound K ml(-1) within 6 h, with a corresponding molar conversion yield of 89 %.
  
  
  CONCLUSIONS
  The recombinant Bgy1 is considered potentially useful for the practical preparation of compound K.
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules, Ministry of Education, Yanbian University, Park Road 977, Yanji, 133002, Jilin, People's Republic of China.
authors.forename: Fei-Liang
authors.initials: FL
authors.lastname: Zhong
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules, Ministry of Education, Yanbian University, Park Road 977, Yanji, 133002, Jilin, People's Republic of China.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Dong
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules, Ministry of Education, Yanbian University, Park Road 977, Yanji, 133002, Jilin, People's Republic of China.
authors.forename: Songquan
authors.initials: S
authors.lastname: Wu
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules, Ministry of Education, Yanbian University, Park Road 977, Yanji, 133002, Jilin, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of Oriental Medicinal Material and Processing, College of Life Science, Korean Ginseng Center Most Valuable Product and Ginseng Genetic Resource Bank, Kyung-Hee University, Seocheon-dong, Giheung-gu, Yongin-Si, Gyeonggi-do, 446-701, Republic of Korea. dcyang@khu.ac.kr.
authors.forename: Deok-Chun
authors.initials: DC
authors.lastname: Yang
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules, Ministry of Education, Yanbian University, Park Road 977, Yanji, 133002, Jilin, People's Republic of China. dhli@ybu.edu.cn.
authors.forename: Donghao
authors.initials: D
authors.lastname: Li
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules, Ministry of Education, Yanbian University, Park Road 977, Yanji, 133002, Jilin, People's Republic of China. lhquan@ybu.edu.cn.
authors.forename: Lin-Hu
authors.initials: LH
authors.lastname: Quan
mesh_terms: D001711:Biotransformation; D036145:Ginsenosides / Q000378:metabolism*; D029774:Gynostemma / Q000378:metabolism; D010936:Plant Extracts / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D001617:beta-Glucosidase / Q000235:genetics / Q000378:metabolism*
chemical_list: D036145:Ginsenosides; D010936:Plant Extracts; D011994:Recombinant Proteins; D001617:beta-Glucosidase; C474918:gypenoside; C112772:ginsenoside compound K
keywords: Biotransformation; Compound K; Gypenoside XVII; β-Glucosidase