title: Expression of the novel adipokine C1qTNF-related protein 4 (CTRP4) suppresses colitis and colitis-associated colorectal cancer in mice.
abstract: Inflammatory bowel disease (IBD) is an important factor in the induction of colon cancer, but its mechanism is unclear. Colitis and colitis-associated colorectal cancer (CAC) models induced using both dextran sulfate sodium (DSS) and the azoxymethane/DSS protocol were established in wild-type (WT) and CTRP4 transgenic (CTRP4-tg) C57BL6/J mice. Body weight, stool consistency and the presence of blood in the stool were analyzed; tumor quantity, size and histological characteristics were analyzed during the development of CAC. The CTRP4-tg mice exhibited significantly reduced colitis and developed far fewer macroscopic tumors; these tumors were smaller in size, and a majority of the colon tumors in these mice were restricted to the superficial mucosa. Tumors of lower grades were observed in the CTRP4-tg mice. Interleukin-6 was markedly downregulated in the CTRP4-tg mice during CAC tumorigenesis. The phosphorylation of ERK, signal transducer and activator of transcription 3 and Akt in the colon and the proliferation of intestinal epithelial cells were decreased in the CTRP4-tg mice. The injection of recombinant CTRP4 protein significantly reduced the colitis symptoms of the WT mice. CTRP4 plays an important role in inflammation and inflammation-associated colon tumorigenesis, and our research may provide a novel method for the treatment of IBD and CAC.
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology (Ministry of Health), School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology (Ministry of Health), School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology (Ministry of Health), School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology (Ministry of Health), School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Tan
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology (Ministry of Health), School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Lanlan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology (Ministry of Health), School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Daxiang
authors.initials: D
authors.lastname: Na
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology (Ministry of Health), School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Guoying
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology (Ministry of Health), School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Ang
authors.initials: A
authors.lastname: Yin
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology (Ministry of Health), School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology (Ministry of Health), School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Xia
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology (Ministry of Health), School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Yingmei
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Xueying
authors.initials: X
authors.lastname: Shi
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology (Ministry of Health), School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
mesh_terms: D054392:Adipokines / Q000378:metabolism*; D000818:Animals; D063646:Carcinogenesis / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D003092:Colitis / Q000139:chemically induced / Q000150:complications* / Q000473:pathology* / Q000517:prevention & control; D015179:Colorectal Neoplasms / Q000150:complications* / Q000473:pathology*; D016207:Cytokines / Q000378:metabolism; D016264:Dextran Sulfate; D015536:Down-Regulation / Q000187:drug effects; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011994:Recombinant Proteins / Q000494:pharmacology; D050796:STAT3 Transcription Factor / Q000378:metabolism; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: D054392:Adipokines; C000621233:Ctrp4 protein, mouse; D016207:Cytokines; D018836:Inflammation Mediators; D015850:Interleukin-6; D011994:Recombinant Proteins; D050796:STAT3 Transcription Factor; D051996:Transcription Factor RelA; D016264:Dextran Sulfate