title: SILICATE UPTAKE AND SILICA SHELL FORMATION BY SYNCHRONOUSLY DIVIDING CELLS OF THE DIATOM NAVICULA PELLICULOSA (BRÉB.) HILSE(1,2,3).
abstract: Cells of the freshwater diatom Navicula pelliculosa were induced to divide synchronously by the addition of excess silicate (20 mg Si/liter) to a culture which had been silicate-starved for 14 hr. After a 3- to 4-hr lag period, characterized by rapid silicate uptake (6.5 mg Si/liter/4 hr) and silica shell formation, the cell number then doubled in the succeeding 2-3 hr. This system promises to have significance in the study of silicon metabolism, transport phenomena, and silica deposition.
authors.affiliation: The Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01.545Department of Oceanography, University of Washington, Seattle, Washington 98105.
authors.forename: W F
authors.initials: WF
authors.lastname: Busby
authors.affiliation: The Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01.545Department of Oceanography, University of Washington, Seattle, Washington 98105.
authors.forename: J
authors.initials: J
authors.lastname: Lewin