title: Initial Assessment of the Molecular Epidemiology of blaNDM-1 in Colombia.
abstract: We report complete genome sequences of four blaNDM-1-harboring Gram-negative multidrug-resistant (MDR) isolates from Colombia. The blaNDM-1 genes were located on 193-kb Inc FIA, 178-kb Inc A/C2, and 47-kb (unknown Inc type) plasmids. Multilocus sequence typing (MLST) revealed that these isolates belong to sequence type 10 (ST10) (Escherichia coli), ST392 (Klebsiella pneumoniae), and ST322 and ST464 (Acinetobacter baumannii and Acinetobacter nosocomialis, respectively). Our analysis identified that the Inc A/C2 plasmid in E. coli contained a novel complex transposon (Tn125 and Tn5393 with three copies of blaNDM-1) and a recombination "hot spot" for the acquisition of new resistance determinants.
authors.affiliation: Department of Medicine, Case Western Reserve University, Cleveland, Ohio, USA Department of Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, Ohio, USA Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA.
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Rojas
authors.affiliation: J. Craig Venter Institute, La Jolla, California, USA.
authors.forename: Meredith S
authors.initials: MS
authors.lastname: Wright
authors.affiliation: International Center for Medical Research and Training, CIDEIM, Cali, Colombia.
authors.forename: Elsa
authors.initials: E
authors.lastname: De La Cadena
authors.affiliation: International Center for Medical Research and Training, CIDEIM, Cali, Colombia.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Motoa
authors.affiliation: Department of Medicine, Case Western Reserve University, Cleveland, Ohio, USA Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA.
authors.forename: Kristine M
authors.initials: KM
authors.lastname: Hujer
authors.affiliation: International Center for Medical Research and Training, CIDEIM, Cali, Colombia.
authors.forename: Maria V
authors.initials: MV
authors.lastname: Villegas
authors.affiliation: J. Craig Venter Institute, La Jolla, California, USA madams@jcvi.org robert.bonomo@va.gov.
authors.forename: Mark D
authors.initials: MD
authors.lastname: Adams
authors.affiliation: Department of Medicine, Case Western Reserve University, Cleveland, Ohio, USA Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio, USA Department of Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, Ohio, USA Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA madams@jcvi.org robert.bonomo@va.gov.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Bonomo
mesh_terms: D000150:Acinetobacter / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D040981:Acinetobacter baumannii / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D003105:Colombia; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology / Q000379:methods*; D058885:Multilocus Sequence Typing; D010957:Plasmids / Q000235:genetics
chemical_list: D004269:DNA, Bacterial