title: Development of Mycoplasma hyopneumoniae Recombinant Vaccines.
abstract: Mycoplasma hyopneumoniae is the etiological agent of swine enzootic pneumonia (EP), a disease that affects swine production worldwide. Vaccination is the most cost-effective strategy for the control and prevention of the disease. Research using genome-based approach has the potential to elucidate the biology and pathogenesis of M. hyopneumoniae and contribute to the development of more effective vaccines. Here, we describe the protocol for developing M. hyopneumoniae recombinant vaccines using reverse vaccinology approaches.
authors.affiliation: Faculdade de Ciências da Saúde, Universidade Federal da Grande Dourados, Rodovia Dourados - Itahum, Km 12 - Cidade Universitaria, Cx. Postal 533, Dourados, MS, CEP 79804-970, Brazil.
authors.forename: Silvana Beutinger
authors.initials: SB
authors.lastname: Marchioro
authors.affiliation: Faculdade de Ciências Biológicas e Ambientais, Universidade Federal da Grande Dourados, Rodovia Dourados - Itahum, Km 12 - Cidade Universitaria, Cx. Postal 533, Dourados, MS, CEP 79804-970, Brazil.
authors.forename: Simone
authors.initials: S
authors.lastname: Simionatto
authors.affiliation: Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Campus Universitário s/n, Prédio 19, Caixa Postal 354, Pelotas, RS, 96010-900, Brazil. odirad@terra.com.br.
authors.forename: Odir
authors.initials: O
authors.lastname: Dellagostin
mesh_terms: D015153:Blotting, Western; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004586:Electrophoresis; D016297:Mutagenesis, Site-Directed; D045705:Mycoplasma hyopneumoniae / Q000235:genetics / Q000276:immunology*; D012995:Solubility; D014614:Vaccines, Synthetic / Q000737:chemistry / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D017931:DNA Primers; D014614:Vaccines, Synthetic
keywords: Mycoplasma hyopneumoniae; Recombinant vaccines; Reverse vaccinology