title: Comparisons of parallel potential biomarkers of 1H-MRS-measured hepatic lipid content in patients with non-alcoholic fatty liver disease.
abstract: Non-alcoholic fatty liver disease (NAFLD) is the main cause of chronic liver disease. This cross-sectional study aimed to evaluate whether parallel clinical features and serum markers are related to the severity of NAFLD. We enrolled 111 participants with different metabolic syndrome (MetS) scores (zero, n = 22; one, n = 19; two, n = 22; and ≥ three, n = 48) and used 1H-MRS to measure liver fat content. Biochemical profiles and potential biomarkers of NAFLD were measured in fasting plasma. We found that 1H-MRS-measured fat content was significantly associated with MetS score ≥1, endotoxin, and hs-CRP. Ordinal logistic regression analysis revealed that MetS score ≥2 and endotoxin were predictive of NAFLD (1H-MRS > 5%) and that endotoxin, hs-CRP, and malondialdehyde (MDA) were predictive of NAFLD with liver injury (1H-MRS > 9.67%). Endotoxin plus MetS score was shown to be the most accurate predictor of overall NAFLD (AUC = 0.854; (95% CI: 0.785-0.924), P < 0.001), and endotoxin plus hs-CRP and MDA was found to be predictive of NAFLD with liver injury (0.868; (0.801-0.936), P < 0.001). These results suggest that MetS score plus certain serum biomarkers with 1H-MRS findings may hold promise for developing an effective model for monitoring the severity of NAFLD.
authors.affiliation: Department of Gastroenterology, Changhua Christian Hospital, Changhua, Taiwan.
authors.forename: Kai-Lun
authors.initials: KL
authors.lastname: Shih
authors.affiliation: Department of Gastroenterology, Changhua Christian Hospital, Changhua, Taiwan.
authors.forename: Wei-Wen
authors.initials: WW
authors.lastname: Su
authors.affiliation: Department of Nephrology, Changhua Christian Hospital, Changhua, Taiwan.|School of Medicine, Chung-Shan Medical University, Taichung, Taiwan.
authors.forename: Chia-Chu
authors.initials: CC
authors.lastname: Chang
authors.affiliation: Internal Medicine Research Center, Changhua Christian Hospital, Changhua, Taiwan.
authors.forename: Chew-Teng
authors.initials: CT
authors.lastname: Kor
authors.affiliation: Department of Medical Imaging, Changhua Christian Hospital, Changhua, Taiwan.
authors.forename: Chen-Te
authors.initials: CT
authors.lastname: Chou
authors.affiliation: Inflammation Research &Drug Development Center, Changhua Christian Hospital, Changhua, Taiwan.
authors.forename: Ting-Yu
authors.initials: TY
authors.lastname: Chen
authors.affiliation: Inflammation Research &Drug Development Center, Changhua Christian Hospital, Changhua, Taiwan.|Department of Neurology, Changhua Christian Hospital, Changhua, Taiwan.|Graduate Institute of Acupuncture Science, China Medical University, Taichung, Taiwan.
authors.forename: Hung-Ming
authors.initials: HM
authors.lastname: Wu
mesh_terms: D015415:Biomarkers; D005260:Female; D006801:Humans; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D024821:Metabolic Syndrome / Q000378:metabolism; D055432:Metabolomics / Q000379:methods; D008875:Middle Aged; D065626:Non-alcoholic Fatty Liver Disease / Q000175:diagnosis / Q000378:metabolism*; D018384:Oxidative Stress; D066244:Proton Magnetic Resonance Spectroscopy; D019655:Quantitative Trait, Heritable; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D015415:Biomarkers; D017382:Reactive Oxygen Species