title: Detection system based on magnetoelastic sensor for classical swine fever virus.
abstract: In this paper, a magnetoelastic (ME) sensing system for the detection of classical swine fever virus (CSFV) is presented. The detection system comprises a test paper and a measurement circuit. The test paper consists mainly of an ME biosensor to detect the CSFV. Based on the impedance analysis technique, the measurement circuit is designed to measure the resonance frequency of the ME biosensor. The anti-CSFV IgG is immobilized onto the ME sensor surface to form the ME biosensor through a physical absorption approach. The experimental results show that the shift in the resonance frequency of the ME biosensor increases with the augmentation of the CSFV concentration. The effectiveness of the combination between the anti-CSFV IgG and CSFV is confirmed by the scanning electron microscopy (SEM) images, the sandwich-based enzyme-linked immunosorbent assay (ELISA) analysis, the interference study and the reference biosensor test method. The resonance frequency shift is linearly proportional to the concentration in the range from 0 to 2.5μg/ml, and becomes sub-linear at higher concentrations. The ME biosensor for CSFV detection has a sensitivity of about 95Hz/μg·ml(-1), with a detection limit of 0.6μg/ml.
authors.affiliation: MicroNano System Research Center, Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education & College of Information Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Guo
authors.affiliation: MicroNano System Research Center, Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education & College of Information Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Gao
authors.affiliation: MicroNano System Research Center, Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education & College of Information Engineering, Taiyuan University of Technology, Taiyuan 030024, China. Electronic address: sangshengbo@tyut.edu.cn.
authors.forename: Shengbo
authors.initials: S
authors.lastname: Sang
authors.affiliation: MicroNano System Research Center, Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education & College of Information Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
authors.forename: Aoqun
authors.initials: A
authors.lastname: Jian
authors.affiliation: MicroNano System Research Center, Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education & College of Information Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Duan
authors.affiliation: MicroNano System Research Center, Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education & College of Information Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
authors.forename: Jianlong
authors.initials: J
authors.lastname: Ji
authors.affiliation: MicroNano System Research Center, Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education & College of Information Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
authors.forename: Wendong
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry; D000914:Antibodies, Viral / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D006691:Classical Swine Fever / Q000175:diagnosis* / Q000821:virology; D006692:Classical Swine Fever Virus / Q000302:isolation & purification*; D017097:Electric Impedance; D004797:Enzyme-Linked Immunosorbent Assay; D010209:Paper; D012680:Sensitivity and Specificity; D013552:Swine
chemical_list: D055910:Antibodies, Immobilized; D000914:Antibodies, Viral
keywords: CSFV; Detection system; ME biosensor; Measurement circuit; Test paper