title: Acanthamoeba genotypes T2, T4, and T11 in soil sources from El Hierro island, Canary Islands, Spain.
abstract: The genus Acanthamoeba includes pathogenic strains which are causative agents of keratitis and encephalitis that often may end fatal in humans and other animals. In the present study, forty soil samples were collected in the island of El Hierro, Canary Islands, Spain, and checked for the presence of Acanthamoeba. Samples were cultivated onto 2 % non-nutrient agar plates seeded with a layer of heat killed Escherichia coli. Amplification by PCR and sequencing of the DF3 region of the 18S rDNA of Acanthamoeba was carried out in order to confirm morphological identification of the amoebae. Furthermore, Acanthamoeba spp. was isolated from 47.5 % of soil samples. Moreover, genotypes T2, T4, and T11 were identified in these samples. To the best of our knowledge, this is the first study to establish genotypes T2, T4, and T11 in soil sources from El Hierro island.
authors.affiliation: University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203, La Laguna, Tenerife, Canary Islands, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Reyes-Batlle
authors.affiliation: University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203, La Laguna, Tenerife, Canary Islands, Spain.
authors.forename: Jonadab
authors.initials: J
authors.lastname: Zamora-Herrera
authors.affiliation: University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203, La Laguna, Tenerife, Canary Islands, Spain.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Vargas-Mesa
authors.affiliation: University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203, La Laguna, Tenerife, Canary Islands, Spain.
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: Valerón-Tejera
authors.affiliation: University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203, La Laguna, Tenerife, Canary Islands, Spain.|Cátedra de Parasitología, Escuela de Bioanálisis, Facultad de Medicina, Universidad Central de Venezuela, Caracas, Venezuela.
authors.forename: Carolina
authors.initials: C
authors.lastname: Wagner
authors.affiliation: University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203, La Laguna, Tenerife, Canary Islands, Spain.
authors.forename: Carmen Ma
authors.initials: CM
authors.lastname: Martín-Navarro
authors.affiliation: University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203, La Laguna, Tenerife, Canary Islands, Spain.
authors.forename: Atteneri
authors.initials: A
authors.lastname: López-Arencibia
authors.affiliation: University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203, La Laguna, Tenerife, Canary Islands, Spain.|Laboratoire Matériaux-Molécules et Applications, IPEST, University of Carthage, La Marsa, Tunisia.
authors.forename: Ines
authors.initials: I
authors.lastname: Sifaoui
authors.affiliation: University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203, La Laguna, Tenerife, Canary Islands, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Martínez-Carretero
authors.affiliation: University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203, La Laguna, Tenerife, Canary Islands, Spain.
authors.forename: Basilio
authors.initials: B
authors.lastname: Valladares
authors.affiliation: University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203, La Laguna, Tenerife, Canary Islands, Spain.
authors.forename: José E
authors.initials: JE
authors.lastname: Piñero
authors.affiliation: University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203, La Laguna, Tenerife, Canary Islands, Spain. JMLORENZ@ULL.EDU.ES.
authors.forename: Jacob
authors.initials: J
authors.lastname: Lorenzo-Morales
mesh_terms: D000048:Acanthamoeba / Q000235:genetics / Q000302:isolation & purification*; D000656:Amoeba / Q000235:genetics; D000818:Animals; D016054:DNA, Protozoan; D004275:DNA, Ribosomal / Q000235:genetics; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D012987:Soil / Q000469:parasitology*; D013030:Spain
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D012987:Soil
keywords: Acanthamoeba; Canary Islands; El Hierro; Genotype; Soil