title: In-vivo immunofluorescent imaging in cases of posterior uveitis.
abstract: In-vitro immunofluorescent assays/imaging are routinely used methods of detecting antigens. The ability to perform ocular angiography to study the choroidal and retinal vasculature in real time provides us with a unique opportunity to perform real time in-vivo immunofluorescent imaging. This unique combination of in-vivo immunofluorescent imaging and live imaging of choroidal and retinal circulation can help detect antigens of infective organisms in-vivo to diagnose causative infective aetiology in cases of choroiditis/retinitis. The following paper describes the basic designing of such an imaging platform.
authors.affiliation: Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi 110029, India. Electronic address: dr.rohanrpc@gmail.com.
authors.forename: Rohan
authors.initials: R
authors.lastname: Chawla
authors.affiliation: Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi 110029, India.
authors.forename: Pradeep
authors.initials: P
authors.lastname: Venkatesh
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology / Q000493:pharmacokinetics; D000937:Antigen-Antibody Reactions; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology; D000946:Antigens, Fungal / Q000032:analysis / Q000276:immunology; D001813:Blood-Retinal Barrier; D002825:Chorioretinitis / Q000000981:diagnostic imaging / Q000276:immunology / Q000382:microbiology; D002829:Choroid / Q000098:blood supply* / Q000000981:diagnostic imaging; D005451:Fluorescein Angiography / Q000379:methods*; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods*; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D005654:Fundus Oculi; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007141:Immunoglobulin Fc Fragments / Q000276:immunology; D007208:Indocyanine Green / Q000493:pharmacokinetics; D011994:Recombinant Proteins / Q000276:immunology / Q000493:pharmacokinetics; D012171:Retinal Vessels / Q000000981:diagnostic imaging*; D015866:Uveitis, Posterior / Q000000981:diagnostic imaging* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000946:Antigens, Fungal; D005456:Fluorescent Dyes; D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D011994:Recombinant Proteins; D007208:Indocyanine Green