title: A Study on the Chemical Compositions of the Yinqiaosan (Lonicerae and Forsythiae Powder) at Different Time of Later-decoction by Gas Chromatography Mass Spectrometry.
abstract: BACKGROUND
  Yinqiaosan (Lonicerae and Forsythiae Powder), as a famous prescription of Dr. Wu Jutong in Qing dynasty of China, has the effects of diaphoresis cooling, fire-purging, and detoxicaton. It is mainly used in the treatment of influenza, hand-foot-mouth disease, esophagitis, pneumonia, acute tonsillitis, mumps, and other viral infections. It is one of the widely used traditional Chinese medicine prescriptions with proven curative effects in clinical use.
  
  
  OBJECTIVE
  To research the material basis of Yinqiaosan decoction when decocting mint, herba schizonepetae in different length of later-decoction time, to find the influence on volatile components of Yinqiaosan decoction decocted later in different length of time, to lay the foundation to further clarify the after-decoction mechanism of Yinqiaosan, and the specification of Yinqiaosan decoction process.
  
  
  METHODS
  Gas chromatography mass spectrometry method is used to analyze the volatile components of Yinqiaosan decoction samples decocted for 0, 3, 5, 8, and 10 min.
  
  
  RESULTS
  Later-decocting mint and herba schizonepetae at different time when decocting Yinqiaosan had a significant influence on the volatile components of the solution. 54 different chemical components were identified: 25 were identified when later-decocting the sample for 3 min; 13 were identified when later-decocting the sample for 5 min; 11 were identified when later-decocting the sample for 8 min; 7 were identified when later-decocting the sample for 10 min; and 26 were identified when later-decocting the sample for 0 min. There were more volatile components in the sample after-decocted for 3 min. A total of 54 different chemical components were identified in different later-decocting solution samples. These components form the basis of the Yinqiaosan drug effect.
  
  
  CONCLUSIONS
  The length of later-decoction time of mint and herba schizonepetae was confirmed to be 3 min when decocting Yinqiaosan.
  
  
  CONCLUSIONS
  Later-decocting mint and herba schizonepetae at different time had a significant influence on the volatile components of the solutionFifty-four different chemical components were identified in different later-decocting solution samplesThere were more volatile components in the sample after-decocted for 3 minThe volatile components content was high. These components form the important basis of the Yinqiaosan drug effect.Total ion flow diagram of volatile oils in the Yinqiaosan sample with mint, herba schizonepetae after 3 min decoction. Abbreviations used: GC-MS: Gas chromatography mass spectrometry, TCM: Traditional Chinese medicine.
authors.affiliation: Department of Pharmacy, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China; Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210046, China.
authors.forename: Yachun
authors.initials: Y
authors.lastname: Shu
authors.affiliation: Department of Pharmacy, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210046, China; Research and Development Center, Nanjing Haichang Chinese Medicine Group Corporation, Nanjing 210061, China.
authors.forename: Kunming
authors.initials: K
authors.lastname: Qin
authors.affiliation: Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210046, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.affiliation: Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210046, China.
authors.forename: Baochang
authors.initials: B
authors.lastname: Cai
keywords: Gas chromatography mass spectrometry; Yinqiaosan decoction; later-decoction; later-decoction time; volatile components