title: A novel self-powered and sensitive label-free DNA biosensor in microbial fuel cell.
abstract: In this work, a novel self-powered, sensitive, low-cost, and label-free DNA biosensor is reported by applying a two-chambered microbial fuel cell (MFC) as a power supply. A graphite electrode and an Au nanoparticles modified graphite electrode (AuNP/graphite electrode) were used as anode and cathode in the MFC system, respectively. The active biocatalyst in the anodic chamber was a mixed culture of microorganisms. The sensing element of the biosensor was fabricated by the well-known Au-thiol binding the ssDNA probe on the surface of an AuNP/graphite cathode. Electrons produced by microorganisms were transported from the anode to the cathode through an external circuit, which could be detected by the terminal multi-meter detector. The difference between power densities of the ssDNA probe modified cathode in the absence and presence of complementary sequence served as the detection signal of the DNA hybridization with detection limit of 3.1nM. Thereafter, this biosensor was employed for diagnosis and determination of complementary sequence in a human serum sample. The hybridization specificity studies further revealed that the developed DNA biosensor could distinguish fully complementary sequences from one-base mismatched and non-complementary sequences.
authors.affiliation: Electroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Asghary
authors.affiliation: Electroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran. Electronic address: j.raoof@umz.ac.ir.
authors.forename: Jahan Bakhsh
authors.initials: JB
authors.lastname: Raoof
authors.affiliation: Biofuel and Renewable Energy Research Center, Department of Chemical Engineering, Babol Noshirvani University of Technology, Babol, Iran.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Rahimnejad
authors.affiliation: Electroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Ojani
mesh_terms: D001674:Bioelectric Energy Sources / Q000191:economics; D015374:Biosensing Techniques / Q000191:economics / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000097:blood; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D006801:Humans; D061925:Immobilized Nucleic Acids / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000379:methods
chemical_list: D061925:Immobilized Nucleic Acids; D006046:Gold; D004247:DNA
keywords: Au nanoparticles; Cathode; DNA biosensor; Microbial fuel cell; Self-powered