title: Functional and bioinformatics analysis of an exopolysaccharide-related gene (epsN) from Lactobacillus kefiranofaciens ZW3.
abstract: A novel lactic acid bacteria strain Lactobacillus kefiranofaciens ZW3 exhibited the characteristics of high production of exopolysaccharide (EPS). The epsN gene, located in the eps gene cluster of this strain, is associated with EPS biosynthesis. Bioinformatics analysis of this gene was performed. The conserved domain analysis showed that the EpsN protein contained MATE-Wzx-like domains. Then the epsN gene was amplified to construct the recombinant expression vector pMG36e-epsN. The results showed that the EPS yields of the recombinants were significantly improved. By determining the yields of EPS and intracellular polysaccharide, it was considered that epsN gene could play its Wzx flippase role in the EPS biosynthesis. This is the first time to prove the effect of EpsN on L. kefiranofaciens EPS biosynthesis and further prove its functional property.
authors.affiliation: Key Laboratory of Food Nutrition and Safety, College of Food Engineering and Biotechnology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China.
authors.forename: Jingrui
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Food Nutrition and Safety, College of Food Engineering and Biotechnology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
authors.affiliation: Key Laboratory of Food Nutrition and Safety, College of Food Engineering and Biotechnology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China.
authors.forename: Yongna
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Food Nutrition and Safety, College of Food Engineering and Biotechnology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China.
authors.forename: Zhuqing
authors.initials: Z
authors.lastname: Xing
authors.affiliation: Key Laboratory of Food Nutrition and Safety, College of Food Engineering and Biotechnology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China. ypwang40@yahoo.com.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D019295:Computational Biology / Q000379:methods*; D007778:Lactobacillus / Q000235:genetics* / Q000378:metabolism; D005810:Multigene Family; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D011135:Polysaccharides, Bacterial
keywords: Bioinformatics analysis; Exopolysaccharide; Flippase; Lactobacillus kefiranofaciens; Wzy-dependent