title: Dynamic Phosphorylation of NudC by Aurora B in Cytokinesis.
abstract: Nuclear distribution protein C (NudC) is a mitotic regulator that plays a role in cytokinesis. However, how NudC is regulated during cytokinesis remains unclear. Here, we show that NudC is phosphorylated by Aurora B, a kinase critical for cell abscission. NudC is co-localized with Aurora B at the midbody and co-immunoprecipitated with Aurora B in mitosis. Inhibition of Aurora B by ZM447439 reduced NudC phosphorylation, suggesting that NudC is an Aurora B substrate in vivo. We identified T40 on NudC as an Aurora B phosphorylation site. NudC depletion resulted in cytokinesis failure with a dramatic elongation of the intercellular bridge between daughter cells, sustained Aurora B activity at the midbody, and reduced cell abscission. These cytokinetic defects can be rescued by the ectopic expression of wild-type NudC. Reconstitution with T40A phospho-defective NudC was found to rescue the cytokinesis defect. In contrast, reconstitution with the T40D phospho-mimetic NudC was inefficient in supporting the completion of cytokinesis. These results suggest that that dynamic phosphorylation of NudC by Aurora B regulates cytokinesis.
authors.affiliation: Program in Integrative Molecular and Biomedical Sciences, Baylor College of Medicine, Houston, Texas, United States of America.
authors.forename: Kimberly N
authors.initials: KN
authors.lastname: Weiderhold
authors.affiliation: Department of Medicine, Section of Allergy Immunology and Rheumatology, Baylor College of Medicine, Houston, Texas, United States of America.
authors.forename: Maria
authors.initials: M
authors.lastname: Fadri-Moskwik
authors.affiliation: Department of Medicine, Section of Allergy Immunology and Rheumatology, Baylor College of Medicine, Houston, Texas, United States of America.
authors.forename: Jing
authors.initials: J
authors.lastname: Pan
authors.affiliation: Program in Integrative Molecular and Biomedical Sciences, Baylor College of Medicine, Houston, Texas, United States of America.
authors.forename: Michiya
authors.initials: M
authors.lastname: Nishino
authors.affiliation: Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, United States of America.
authors.forename: Carol
authors.initials: C
authors.lastname: Chuang
authors.affiliation: Department of Medicine, Section of Allergy Immunology and Rheumatology, Baylor College of Medicine, Houston, Texas, United States of America.
authors.forename: Arpaporn
authors.initials: A
authors.lastname: Deeraksa
authors.affiliation: Department of Translational Molecular Pathology, The University of Texas M.D. Anderson Cancer Center, Houston, Texas, United States of America.
authors.forename: Sue-Hwa
authors.initials: SH
authors.lastname: Lin
authors.affiliation: Program in Integrative Molecular and Biomedical Sciences, Baylor College of Medicine, Houston, Texas, United States of America.|Department of Medicine, Section of Allergy Immunology and Rheumatology, Baylor College of Medicine, Houston, Texas, United States of America.|Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, United States of America.
authors.forename: Li-Yuan
authors.initials: LY
authors.lastname: Yu-Lee
mesh_terms: D064107:Aurora Kinase B / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D048749:Cytokinesis / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis / Q000502:physiology*; D009687:Nuclear Proteins / Q000378:metabolism*; D010766:Phosphorylation
chemical_list: D018797:Cell Cycle Proteins; C512648:NUDC protein, human; D009687:Nuclear Proteins; D064107:Aurora Kinase B