title: Resveratrol improves reproductive parameters of adult rats varicocelized in peripuberty.
abstract: The aim of this study was to investigate the protective action of resveratrol against the reproductive damage caused by left-sided experimental varicocele. There was a reduction of testicular major axis in the varicocele group when compared with the other groups; the testicular volume was reduced in varicocele group in comparison to the sham-control and resveratrol groups. The frequency of morphologically abnormal sperm was higher in varicocele and varicocele treated with resveratrol groups than in sham-control and resveratrol groups. The frequency of sperm with 100% of mitochondrial activity and normal acrosome integrity were lower in varicocele group than in varicocele treated with resveratrol, sham-control and resveratrol groups. Sperm motility was also reduced in varicocele group than in other groups. The sperm DNA fragmentation was higher in varicocele group than in other groups. Testicular levels of malondialdehyde were higher in varicocele and varicocele treated with resveratrol groups. The varicocele and varicocele treated with resveratrol groups had a significantly higher frequency of TUNEL-positive cells than sham-control and resveratrol groups; however, immunolabeling of the testes from varicocele treated with resveratrol group showed a lower number of apoptotic germ cells in comparison with the left testis of rats of the varicocele group. Reproductive alterations produced by varicocele from peripuberty were reduced by resveratrol in adulthood. Resveratrol should be better investigated as an adjuvant in the treatment of varicocele. Daily administration of resveratrol to rats with varicocele from peripuberty improves sperm quality in the adulthood.
authors.affiliation: Laboratory of Developmental BiologyDepartment of Morphology and Genetics, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil.
authors.forename: Talita Biude
authors.initials: TB
authors.lastname: Mendes
authors.affiliation: Laboratory of Developmental BiologyDepartment of Morphology and Genetics, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil.
authors.forename: Camila Cicconi
authors.initials: CC
authors.lastname: Paccola
authors.affiliation: Laboratory of Developmental BiologyDepartment of Morphology and Genetics, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil.
authors.forename: Flávia Macedo
authors.initials: FM
authors.lastname: de Oliveira Neves
authors.affiliation: Laboratory of Developmental BiologyDepartment of Morphology and Genetics, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil.
authors.forename: Joana Noguères
authors.initials: JN
authors.lastname: Simas
authors.affiliation: Laboratory of Developmental BiologyDepartment of Morphology and Genetics, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil.
authors.forename: André
authors.initials: A
authors.lastname: da Costa Vaz
authors.affiliation: Laboratory of Developmental BiologyDepartment of Morphology and Genetics, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil.
authors.forename: Regina Elisabeth L
authors.initials: RE
authors.lastname: Cabral
authors.affiliation: Laboratory of Developmental BiologyDepartment of Morphology and Genetics, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Vendramini
authors.affiliation: Laboratory of Developmental BiologyDepartment of Morphology and Genetics, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil miraglia.sm@gmail.com.
authors.forename: Sandra Maria
authors.initials: SM
authors.lastname: Miraglia
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D007248:Infertility, Male / Q000517:prevention & control*; D008297:Male; D011627:Puberty / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012098:Reproduction / Q000502:physiology*; D000077185:Resveratrol; D013081:Sperm Motility / Q000187:drug effects*; D013267:Stilbenes / Q000494:pharmacology*; D013737:Testis / Q000187:drug effects / Q000502:physiology*; D014646:Varicocele
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D013267:Stilbenes; D000077185:Resveratrol