title: Complete genome sequences of Francisella noatunensis subsp. orientalis strains FNO12, FNO24 and FNO190: a fish pathogen with genomic clonal behavior.
abstract: The genus Francisella is composed of Gram-negative, pleomorphic, strictly aerobic and non-motile bacteria, which are capable of infecting a variety of terrestrial and aquatic animals, among which Francisella noatunensis subsp. orientalis stands out as the causative agent of pyogranulomatous and granulomatous infections in fish. Accordingly, F. noatunensis subsp. orientalis is responsible for high mortality rates in freshwater fish, especially Nile Tilapia. In the current study, we present the genome sequences of F. noatunensis subsp. orientalis strains FNO12, FNO24 and FNO190. The genomes include one circular chromosome of 1,859,720 bp, consisting of 32 % GC content, 1538 coded proteins and 363 pseudogenes for FNO12; one circular chromosome of 1,862,322 bp, consisting of 32 % GC content, 1537 coded proteins and 365 pseudogenes for FNO24; and one circular chromosome of 1,859,595 bp, consisting of 32 % GC content, 1539 coded proteins and 362 pseudogenes for FNO190. All genomes have similar genetic content, implicating a clonal-like behavior for this species.
authors.affiliation: National Reference Laboratory for Aquatic Animal Diseases (AQUACEN), Ministry of Fisheries and Aquaculture, Federal University of Minas Gerais, Belo Horizonte, Brazil ; Laboratory of Cellular and Molecular Genetics (LGCM), Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Lucas Amorim
authors.initials: LA
authors.lastname: Gonçalves
authors.affiliation: National Reference Laboratory for Aquatic Animal Diseases (AQUACEN), Ministry of Fisheries and Aquaculture, Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Siomar
authors.initials: S
authors.lastname: de Castro Soares
authors.affiliation: National Reference Laboratory for Aquatic Animal Diseases (AQUACEN), Ministry of Fisheries and Aquaculture, Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Felipe Luiz
authors.initials: FL
authors.lastname: Pereira
authors.affiliation: National Reference Laboratory for Aquatic Animal Diseases (AQUACEN), Ministry of Fisheries and Aquaculture, Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Fernanda Alves
authors.initials: FA
authors.lastname: Dorella
authors.affiliation: National Reference Laboratory for Aquatic Animal Diseases (AQUACEN), Ministry of Fisheries and Aquaculture, Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Alex Fiorini
authors.initials: AF
authors.lastname: de Carvalho
authors.affiliation: National Reference Laboratory for Aquatic Animal Diseases (AQUACEN), Ministry of Fisheries and Aquaculture, Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Gabriel Magno
authors.initials: GM
authors.lastname: de Freitas Almeida
authors.affiliation: National Reference Laboratory for Aquatic Animal Diseases (AQUACEN), Ministry of Fisheries and Aquaculture, Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Carlos Augusto Gomes
authors.initials: CA
authors.lastname: Leal
authors.affiliation: Laboratory of Cellular and Molecular Genetics (LGCM), Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Vasco
authors.initials: V
authors.lastname: Azevedo
authors.affiliation: National Reference Laboratory for Aquatic Animal Diseases (AQUACEN), Ministry of Fisheries and Aquaculture, Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Henrique César Pereira
authors.initials: HC
authors.lastname: Figueiredo
keywords: Complete genome sequencing; Fish pathogen; Genetic clonal behavior