title: The Powdering Process with a Set of Ceramic Mills for Green Tea Promoted Catechin Extraction and the ROS Inhibition Effect.
abstract: For serving green tea, there are two prominent methods: steeping the leaf or the powdered leaf (matcha style) in hot water. The purpose of the present study was to reveal chemical and functional differences before and after the powdering process of green tea leaf, since powdered green tea may contribute to expanding the functionality because of the different ingesting style. In this study, we revealed that the powdering process with a ceramic mill and stirring in hot water increased the average extracted concentration of epigallocatechin gallate (EGCG) by more than three times compared with that in leaf tea using high-performance liquid chromatography (HPLC) and liquid chromatography-tandem mass Spectrometry (LC-MS/MS) analyses. Moreover, powdered green tea has a higher inhibition effect of reactive oxygen species (ROS) production in vitro compared with the same amount of leaf tea. Our data suggest that powdered green tea might have a different function from leaf tea due to the higher catechin contents and particles.
authors.affiliation: Division of Molecular Cell Biology, Core Research Facilities for Basic Science, The Jikei University School of Medicine, Tokyo 105-8461, Japan. kfujioka@jikei.ac.jp.
authors.forename: Kouki
authors.initials: K
authors.lastname: Fujioka
authors.affiliation: Division of Molecular Cell Biology, Core Research Facilities for Basic Science, The Jikei University School of Medicine, Tokyo 105-8461, Japan. tmiwamoto@jikei.ac.jp.
authors.forename: Takeo
authors.initials: T
authors.lastname: Iwamoto
authors.affiliation: Health and Environment Systems Division, Consumer Electronics Company, Sharp Corporation, Osaka 581-8585, Japan. shima.hidekazu@sharp.co.jp.
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Shima
authors.affiliation: Division of Molecular Cell Biology, Core Research Facilities for Basic Science, The Jikei University School of Medicine, Tokyo 105-8461, Japan. tomaru@jikei.ac.jp.
authors.forename: Keiko
authors.initials: K
authors.lastname: Tomaru
authors.affiliation: Division of Molecular Cell Biology, Core Research Facilities for Basic Science, The Jikei University School of Medicine, Tokyo 105-8461, Japan. h-saito@jikei.ac.jp.
authors.forename: Hideki
authors.initials: H
authors.lastname: Saito
authors.affiliation: Health and Environment Systems Division, Consumer Electronics Company, Sharp Corporation, Osaka 581-8585, Japan. ohtsuka.masaki@sharp.co.jp.
authors.forename: Masaki
authors.initials: M
authors.lastname: Ohtsuka
authors.affiliation: Health and Environment Systems Division, Consumer Electronics Company, Sharp Corporation, Osaka 581-8585, Japan. yoshidome.akihiro@sharp.co.jp.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Yoshidome
authors.affiliation: Health and Environment Systems Division, Consumer Electronics Company, Sharp Corporation, Osaka 581-8585, Japan. tynkerbell1218@hotmail.com.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Kawamura
authors.affiliation: Division of Molecular Cell Biology, Core Research Facilities for Basic Science, The Jikei University School of Medicine, Tokyo 105-8461, Japan. manome@jikei.ac.jp.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Manome
mesh_terms: D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D006358:Hot Temperature; D018515:Plant Leaves / Q000737:chemistry*; D011208:Powders / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000737:chemistry*; D053719:Tandem Mass Spectrometry; D013662:Tea / Q000737:chemistry*; D014867:Water
chemical_list: D011208:Powders; D017382:Reactive Oxygen Species; D013662:Tea; D014867:Water; D002392:Catechin; C045651:epigallocatechin gallate
keywords: ROS; catechin; green tea; matcha; mill; polyphenol; powdering process