title: Structure elucidation of a bioactive polysaccharide from fruiting bodies of Hericium erinaceus in different maturation stages.
abstract: HPB-3, a heteropolysaccharide, with a mean molecular weight of 1.5×10(4)Da, was obtained from the maturating-stage IV, V and VI fruiting body of Hericium erinaceus, exhibited higher macrophages stimulation activities, was able to upregulate the functional events mediated by activated macrophages, such as production of nitric oxide (NO). Monosaccharide composition analysis showed that HPB-3 comprised l-fucose, d-galactose and d-glucose in the ratio of 5.2:23.9:1. Its chemical structure was characterized by sugar and methylation analysis, along with (1)H and (13)C NMR spectroscopy, including (1)H-(1)H COSY, TOCSY, NOESY, HMQC and HMBC experiments. The results indicated that HPB-3 contained a-(1/6)-linked galactopyranosyl backbone, partially with a side chain composed of α-l-fucopyranose at the O-2 position. The predicted primary structure of the polysaccharide was established as below.
authors.affiliation: Shanghai Academy of Agricultural Sciences, Institute of Edible Fungi, Shanghai 201403, PR China; National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization, South, Ministry of Agriculture, PR China.
authors.forename: Qiao-Zhen
authors.initials: QZ
authors.lastname: Li
authors.affiliation: Shanghai Academy of Agricultural Sciences, Institute of Edible Fungi, Shanghai 201403, PR China; National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization, South, Ministry of Agriculture, PR China.
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.affiliation: Shanghai Academy of Agricultural Sciences, Institute of Edible Fungi, Shanghai 201403, PR China; National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization, South, Ministry of Agriculture, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Shanghai Academy of Agricultural Sciences, Institute of Edible Fungi, Shanghai 201403, PR China; National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization, South, Ministry of Agriculture, PR China.
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Liu
authors.affiliation: Shanghai Academy of Agricultural Sciences, Institute of Edible Fungi, Shanghai 201403, PR China; National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization, South, Ministry of Agriculture, PR China.
authors.forename: Zheng-Peng
authors.initials: ZP
authors.lastname: Li
authors.affiliation: Shanghai Academy of Agricultural Sciences, Institute of Edible Fungi, Shanghai 201403, PR China; National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization, South, Ministry of Agriculture, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Feng
authors.affiliation: Shanghai Academy of Agricultural Sciences, Institute of Edible Fungi, Shanghai 201403, PR China; National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization, South, Ministry of Agriculture, PR China. Electronic address: yangyan@saas.sh.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000818:Animals; D001487:Basidiomycota / Q000737:chemistry* / Q000254:growth & development*; D048690:Fruiting Bodies, Fungal / Q000737:chemistry* / Q000254:growth & development; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D009569:Nitric Oxide / Q000096:biosynthesis; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000067996:RAW 264.7 Cells
chemical_list: D011134:Polysaccharides; D009569:Nitric Oxide
keywords: Hericium erinaceus; Macrophage activation; Maturation stage; Polysaccharide; Structural analysis