title: Chemo-enzymatic labeling for rapid assignment of RNA molecules.
abstract: Even though Nuclear Magnetic Resonance (NMR) spectroscopy is one of the few techniques capable of determining atomic resolution structures of RNA, it is constrained by two major problems of chemical shift overlap of resonances and rapid signal loss due to line broadening. Emerging tools to tackle these problems include synthesis of atom specifically labeled or chemically modified nucleotides. Herein we review the synthesis of these nucleotides, the design and production of appropriate RNA samples, and the application and analysis of the NMR experiments that take advantage of these labels.
authors.affiliation: Department of Chemistry & Biochemistry, University of Maryland, Biomolecular Sciences Building (296), 8314 Paint Branch Dr., College Park, MD 20782, USA.
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Longhini
authors.affiliation: Department of Chemistry & Biochemistry, University of Maryland, Biomolecular Sciences Building (296), 8314 Paint Branch Dr., College Park, MD 20782, USA.
authors.forename: Regan M
authors.initials: RM
authors.lastname: LeBlanc
authors.affiliation: Department of Chemistry & Biochemistry, University of Maryland, Biomolecular Sciences Building (296), 8314 Paint Branch Dr., College Park, MD 20782, USA. Electronic address: dayie@umd.edu.
authors.forename: T Kwaku
authors.initials: TK
authors.lastname: Dayie
mesh_terms: D000577:Amides / Q000737:chemistry; D001483:Base Sequence; D055029:Inverted Repeat Sequences; D007553:Isotope Labeling; D009682:Magnetic Resonance Spectroscopy; D010756:Phosphoric Acids / Q000737:chemistry; D011687:Purines / Q000737:chemistry; D011743:Pyrimidines / Q000737:chemistry; D012313:RNA / Q000138:chemical synthesis*
chemical_list: D000577:Amides; D010756:Phosphoric Acids; D011687:Purines; D011743:Pyrimidines; D012313:RNA; C011067:phosphoramidic acid
keywords: A-site; Atomic-, site-selective isotopic labels; Filtered/edited NOESY; NMR; RNA