title: Fibronectin-modified surfaces for evaluating the influence of cell adhesion on sensitivity of leukemic cells to siRNA nanoparticles.
abstract: OBJECTIVE
  This study aimed to create fibronectin (FN)-grafted polymeric surfaces to investigate the influence of leukemic cell adhesion on siRNA treatment.
  
  
  METHODS
  FN was grafted on plasma-treated PTFE surfaces using chemical crosslinkers. Adhesion and growth of chronic myeloid leukemia K562 cells on modified surfaces were investigated. The silencing effect of siRNA/lipid-polymers nanoparticles on cells grown on FN-grafted surfaces was evaluated.
  
  
  RESULTS
  Crosslinker-mediated immobilization showed significant FN grafting on surfaces, which provided K562 cell adhesion and growth advantage. siRNA nanoparticle silencing was similarly effective on FN-adhered and suspension-growing K562 cells.
  
  
  CONCLUSIONS
  This study provided initial data to develop a cell-adhesive system to investigate therapeutic effects on leukemic cells. The response of chronic myeloid leukemia cells to siRNA nanoparticles was independent on cell attachment.
authors.affiliation: Department of Biomedical Engineering, Faculty of Medicine & Dentistry, University of Alberta, Edmonton, Alberta T6G 2V2, Canada.
authors.forename: Juliana
authors.initials: J
authors.lastname: Valencia-Serna
authors.affiliation: Département de génie des mines, de la métallurgie et des Matériaux & Centre de recherche du CHU de Québec, Université Laval, Québec, Québec G1L 3L5, Canada.
authors.forename: Pascale
authors.initials: P
authors.lastname: Chevallier
authors.affiliation: Department of Chemical & Materials Engineering, Faculty of Engineering, University of Alberta, Edmonton, Alberta T6G 2G6, Canada.
authors.forename: Remant Bahadur
authors.initials: RB
authors.lastname: Kc
authors.affiliation: Département de génie des mines, de la métallurgie et des Matériaux & Centre de recherche du CHU de Québec, Université Laval, Québec, Québec G1L 3L5, Canada.
authors.forename: Gaétan
authors.initials: G
authors.lastname: Laroche
authors.affiliation: Department of Biomedical Engineering, Faculty of Medicine & Dentistry, University of Alberta, Edmonton, Alberta T6G 2V2, Canada.|Department of Chemical & Materials Engineering, Faculty of Engineering, University of Alberta, Edmonton, Alberta T6G 2G6, Canada.|Faculty of Pharmacy & Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta T6G 2G6, Canada.
authors.forename: Hasan
authors.initials: H
authors.lastname: Uludağ
mesh_terms: D001672:Biocompatible Materials / Q000008:administration & dosage / Q000737:chemistry; D002448:Cell Adhesion / Q000235:genetics; D005353:Fibronectins / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D005353:Fibronectins; D034741:RNA, Small Interfering
keywords: biomaterial; cationic polymer; chronic myeloid leukemia; drug sensitivity; fibronectin; molecule grafting; polyethylenimine; siRNA delivery; surface modification; transfection