title: Genome-Wide Association Mapping of Acid Soil Resistance in Barley (Hordeum vulgare L.).
abstract: Genome-wide association studies (GWAS) based on linkage disequilibrium (LD) have been used to detect QTLs underlying complex traits in major crops. In this study, we collected 218 barley (Hordeum vulgare L.) lines including wild barley and cultivated barley from China, Canada, Australia, and Europe. A total of 408 polymorphic markers were used for population structure and LD analysis. GWAS for acid soil resistance were performed on the population using a general linkage model (GLM) and a mixed linkage model (MLM), respectively. A total of 22 QTLs (quantitative trait loci) were detected with the GLM and MLM analyses. Two QTLs, close to markers bPb-1959 (133.1 cM) and bPb-8013 (86.7 cM), localized on chromosome 1H and 4H respectively, were consistently detected in two different trials with both the GLM and MLM analyses. Furthermore, bPb-8013, the closest marker to the major Al(3+) resistance gene HvAACT1 in barley, was identified to be QTL5. The QTLs could be used in marker-assisted selection to identify and pyramid different loci for improved acid soil resistance in barley.
authors.affiliation: Western Barley Genetics Alliance, Department of Agriculture and Food, Government of Western Australia, PerthWA, Australia; Western Barley Genetics Alliance, Murdoch University, MurdochWA, Australia.
authors.forename: Gaofeng
authors.initials: G
authors.lastname: Zhou
authors.affiliation: Western Barley Genetics Alliance, Department of Agriculture and Food, Government of Western Australia, Perth WA, Australia.
authors.forename: Sue
authors.initials: S
authors.lastname: Broughton
authors.affiliation: Western Barley Genetics Alliance, Murdoch University, Murdoch WA, Australia.
authors.forename: Xiao-Qi
authors.initials: XQ
authors.lastname: Zhang
authors.affiliation: TIA - Tasmanian Institute of Agriculture, University of Tasmania, Kings Meadows TAS, Australia.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Ma
authors.affiliation: TIA - Tasmanian Institute of Agriculture, University of Tasmania, Kings Meadows TAS, Australia.
authors.forename: Meixue
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Western Barley Genetics Alliance, Department of Agriculture and Food, Government of Western Australia, PerthWA, Australia; Western Barley Genetics Alliance, Murdoch University, MurdochWA, Australia.
authors.forename: Chengdao
authors.initials: C
authors.lastname: Li
keywords: GWAS; acid soil resistance; aluminum resistance; association mapping; barley