title: Clarithromycin Dose-Dependently Stabilizes Rat Peritoneal Mast Cells.
abstract: BACKGROUND
  Macrolides, such as clarithromycin, have antiallergic properties. Since exocytosis in mast cells is detected electrophysiologically via changes in membrane capacitance (Cm), the absence of such changes due to the drug indicates its mast cell-stabilizing effect.
  
  
  METHODS
  Employing the whole-cell patch clamp technique in rat peritoneal mast cells, we examined the effects of clarithromycin on Cm during exocytosis. Using a water-soluble fluorescent dye, we also examined its effect on deformation of the plasma membrane.
  
  
  RESULTS
  Clarithromycin (10 and 100 μM) significantly inhibited degranulation from mast cells and almost totally suppressed the GTP-x03B3;-S-induced increase in Cm. It washed out the trapping of the dye on the surface of mast cells.
  
  
  CONCLUSIONS
  This study provides for the first time electrophysiological evidence that clarithromycin dose-dependently inhibits the process of exocytosis. The mast cell-stabilizing action of clarithromycin may be attributable to its counteractive effect on plasma membrane deformation induced by exocytosis.
authors.forename: Itsuro
authors.initials: I
authors.lastname: Kazama
authors.forename: Kazutomo
authors.initials: K
authors.lastname: Saito
authors.forename: Asuka
authors.initials: A
authors.lastname: Baba
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Mori
authors.forename: Nozomu
authors.initials: N
authors.lastname: Abe
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Endo
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Toyama
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Ejima
authors.forename: Mitsunobu
authors.initials: M
authors.lastname: Matsubara
authors.forename: Masanori
authors.initials: M
authors.lastname: Yamauchi
mesh_terms: D000818:Animals; D017291:Clarithromycin / Q000494:pharmacology*; D005089:Exocytosis / Q000187:drug effects*; D005456:Fluorescent Dyes / Q000737:chemistry; D008297:Male; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D018613:Microscopy, Confocal; D018408:Patch-Clamp Techniques; D010537:Peritoneum / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005456:Fluorescent Dyes; D017291:Clarithromycin