title: Yi Qi Qing Re Gao-containing serum inhibits lipopolysaccharide-induced rat mesangial cell proliferation by suppressing the Wnt pathway and TGF-β1 expression.
abstract: The aim of the present study was to investigate the effect of Yi Qi Qing Re Gao-containing serum (YQ-S) on rat mesangial cell (MC) proliferation and to investigate the underlying mechanism. MCs were divided into the control, lipopolysaccharide (LPS)-stimulated, YQ-S and fosinopril-containing serum (For-S) groups, and cultured for 48 h. An MTT assay was used to evaluate the proliferation of MCs. In addition, reverse transcription-quantitative polymerase chain reaction and western blot analysis were conducted to detect the expression levels of Wnt4, β-catenin and transforming growth factor (TGF)-β1 in MCs. The results indicated that YQ-S inhibited LPS-induced MC proliferation. The Wnt4 and TGF-β1 mRNA expression levels were reduced in the YQ-S group (P<0.01 or P<0.05). Furthermore, the Wnt4, β-catenin and TGF-β1 protein expression levels were suppressed in the YQ-S group (P<0.01 or P<0.05). Therefore, YQ-S appears to inhibit MC proliferation, and its mechanism may involve the inhibition of the Wnt signaling pathway and downregulation of TGF-β1 expression.
authors.affiliation: Department of Nephrology, Guang'Anmen Hospital of China Academy of Traditional Chinese Medicine Sciences, Beijing 100053, P.R. China.
authors.forename: Liping
authors.initials: L
authors.lastname: Yang
authors.affiliation: Beijing University of Chinese Medicine, Chaoyang, Liaoning 100029, P.R. China.
authors.forename: Xueyan
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Nephrology, Guang'Anmen Hospital of China Academy of Traditional Chinese Medicine Sciences, Beijing 100053, P.R. China.
authors.forename: Yongli
authors.initials: Y
authors.lastname: Zhan
authors.affiliation: Beijing University of Chinese Medicine, Chaoyang, Liaoning 100029, P.R. China.
authors.forename: Huijie
authors.initials: H
authors.lastname: Liu
authors.affiliation: Beijing University of Chinese Medicine, Chaoyang, Liaoning 100029, P.R. China.
authors.forename: Yumin
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Beijing University of Chinese Medicine, Chaoyang, Liaoning 100029, P.R. China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Mao
authors.affiliation: Department of Pharmacology, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing 100029, P.R. China.
authors.forename: X I
authors.initials: XI
authors.lastname: Dong
authors.affiliation: Department of Pharmacology, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing 100029, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
keywords: Wnt signaling pathway; Yi Qi Qing Re Gao; lipopolysaccharide; mesangial cell proliferation; transforming growth factor-β1