title: Synthesis, characterization, cytotoxicity and antiangiogenic activity of copper(II) complexes with 1-adamantoyl hydrazone bearing pyridine rings.
abstract: Three novel copper complexes with tridentate N2O ligand di(2-pyridil) ketone 1-adamantoyl hydrazone (Addpy) of the formula [Cu(II)2Cu(I)2(Addpy)2Br2(μ-Br4)] (1), catena-poly[CuCl(μ-Addpy)(μ-Cl)CuCl2]n (2) and [Cu(Addpy)(NCS)2] (3) were synthesized. Complexes are characterized by X-ray crystallography, spectral (UV-Vis, FTIR), electrochemical (CV) analyses, and magnetochemical measurements. Investigation of anticancer potential of Cu(II) complexes, mode of cell death, apoptosis, and inhibition of angiogenesis were performed. All tested malignant cell lines (HeLa, LS174, A549, K562, and MDA-MB-231) showed high sensitivity to the examined Cu(II) complexes. It has been shown that the complexes induce apoptosis in the caspase 3-dependent manner, whereas the anti-angiogenic effects of 1, 2, and 3 have been confirmed in EA.hy926 cells using a tube formation assay.
authors.affiliation: University of Novi Sad, Faculty of Sciences, Trg D. Obradovića 3, 21000, Novi Sad, Serbia.
authors.forename: Marko V
authors.initials: MV
authors.lastname: Rodić
authors.affiliation: University of Novi Sad, Faculty of Sciences, Trg D. Obradovića 3, 21000, Novi Sad, Serbia. Electronic address: vukadin.leovac@dh.uns.ac.rs.
authors.forename: Vukadin M
authors.initials: VM
authors.lastname: Leovac
authors.affiliation: University of Novi Sad, Faculty of Sciences, Trg D. Obradovića 3, 21000, Novi Sad, Serbia.
authors.forename: Ljiljana S
authors.initials: LS
authors.lastname: Jovanović
authors.affiliation: University of Belgrade, Institute of Nuclear Sciences Vinča, Condensed Matter Physics Laboratory, P.O. Box 522, 11001, Belgrade, Serbia.
authors.forename: Vojislav
authors.initials: V
authors.lastname: Spasojević
authors.affiliation: University of Kragujevac, Faculty of Science, Department of Chemistry, R. Domanovića 12, 34000, Kragujevac, Serbia.
authors.forename: Milan D
authors.initials: MD
authors.lastname: Joksović
authors.affiliation: Institute of Oncology and Radiology of Serbia, Pasterova 14, 11000, Belgrade, Serbia.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Stanojković
authors.affiliation: Institute of Oncology and Radiology of Serbia, Pasterova 14, 11000, Belgrade, Serbia.
authors.forename: Ivana Z
authors.initials: IZ
authors.lastname: Matić
authors.affiliation: University of Novi Sad, Faculty of Sciences, Trg D. Obradovića 3, 21000, Novi Sad, Serbia.
authors.forename: Ljiljana S
authors.initials: LS
authors.lastname: Vojinović-Ješić
authors.affiliation: University of Kragujevac, Faculty of Science, Department of Chemistry, R. Domanovića 12, 34000, Kragujevac, Serbia.
authors.forename: Violeta
authors.initials: V
authors.lastname: Marković
mesh_terms: D000218:Adamantane / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D020533:Angiogenesis Inhibitors / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003300:Copper / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D006835:Hydrazones / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D009389:Neovascularization, Pathologic / Q000188:drug therapy* / Q000473:pathology; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D011725:Pyridines / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
chemical_list: C000612730:1-adamantoyl hydrazone; D020533:Angiogenesis Inhibitors; D000970:Antineoplastic Agents; D006835:Hydrazones; D009942:Organometallic Compounds; D011725:Pyridines; D003300:Copper; C023666:pyridine; D000218:Adamantane
keywords: Angiogenesis; Caspase-3; Copper(II) complexes; Hydrazones; Physico-chemical characterization