title: Development of a Teat Bio-sealant and Evaluation of its Technological and Functional Properties.
abstract: A teat bio-sealant was developed using Weissella cibaria, and the bio-sealant's technological and functional properties were assessed. The development included four experimental phases that were analyzed using independent experimental designs. Initially, sterilized or pasteurized Aloe vera gels were used, and the effect of heat treatment was investigated. In the second phase, the effects of time, storage temperature, and addition of cryopreservatives on the viability of the probiotic were observed. The third phase consisted of evaluating the synergistic effects of the cryopreservatives. The fourth phase involved selecting a material that would provide viscosity to the teat sealant. Technological and functional properties were measured in terms of viability of W. cibaria, and antimicrobial activity against Staphylococcus aureus and Streptococcus agalactiae was also analyzed. A mixture of milk powder and glycerol preserved this antimicrobial activity. Pullulan provided greater viscosity and maintained the technological and functional properties of the bio-sealant for 29 days. This teat bio-sealant can be used as an alternative for the prevention of bovine mastitis.
authors.affiliation: Facultad de Ingeniería y Administración, Universidad Nacional de Colombia, Palmira, Cra 32 No 12 - 00 Vía, Candelaria, Colombia. lserna@unal.edu.co.
authors.forename: Liliana
authors.initials: L
authors.lastname: Serna-Cock
authors.affiliation: Facultad de Ciencias Agropecuarias, Universidad Nacional de Colombia, Palmira, Cra 32 No 12 - 00 Vía, Candelaria, Colombia.
authors.forename: Omar Vladimir
authors.initials: OV
authors.lastname: Pabón-Rodríguez
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002417:Cattle; D003612:Dairying / Q000379:methods*; D005260:Female; D008321:Mammary Glands, Animal / Q000382:microbiology; D008414:Mastitis, Bovine / Q000517:prevention & control*; D013203:Staphylococcal Infections / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000187:drug effects; D013292:Streptococcus agalactiae / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents
keywords: Cryopreservative; Probiotic; Teat sealant; Weissella cibaria