title: Applications of MALDI-TOF MS in environmental microbiology.
abstract: Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) is an emerging technique for microbial identification, characterization, and typing. The single colony method can be used for obtaining a protein fingerprint or profile unique to each microorganism. This technique has been mainly used in the clinical field, but it also has significant potential in the environmental field. The applications of MALDI-TOF MS in environmental microbiology are discussed in this review. An overview on the use of MALDI-TOF MS for environmental proteomics and metabolomics is given as well as its use for bacterial strain typing and bioremediation research. A more detailed review on the use of this technique for the identification, differentiation, and categorization of environmental microorganisms is given. Some of the parameters that can influence the results and reproducibility of MALDI-TOF MS are also discussed.
authors.affiliation: Department of Chemistry and Biochemistry, The University of Texas at Arlington, Arlington, TX, USA. kschug@uta.edu.
authors.forename: Inês C
authors.initials: IC
authors.lastname: Santos
authors.affiliation: Inform Environmental, LLC, Dallas, TX, USA and Affiliate of the Collaborative Laboratories for Environmental Analysis and Remediation, The University of Texas at Arlington, Arlington, TX, USA.
authors.forename: Zacariah L
authors.initials: ZL
authors.lastname: Hildenbrand
authors.affiliation: Department of Chemistry and Biochemistry, The University of Texas at Arlington, Arlington, TX, USA. kschug@uta.edu and Affiliate of the Collaborative Laboratories for Environmental Analysis and Remediation, The University of Texas at Arlington, Arlington, TX, USA.
authors.forename: Kevin A
authors.initials: KA
authors.lastname: Schug
mesh_terms: D015373:Bacterial Typing Techniques; D004783:Environmental Microbiology; D040901:Proteomics; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization