title: Development of CpG ODN Based Vaccine Adjuvant Formulations.
abstract: Development of effective vaccine mediated immune responses relies on the use of vaccine adjuvants capable of enhancing and directing the adaptive immune response to the antigen. When used as vaccine adjuvants, type I interferon inducing agents can elicit potent effector/memory T cell responses and humoral immunity. Distinct sequences of single stranded synthetic oligodeoxynucleotides containing unmethylated cytosine-phosphate-guanine oligodeoxynucleotide motifs (CpG ODN) can generate type I interferon production via a TLR9-MyD88-IRF7-mediated signaling pathway. Here, we describe two different methods of preparing CpG ODN-based vaccine adjuvant formulations that can induce a robust IFNα response from human peripheral blood mononuclear cells.
authors.affiliation: Department of Biological Sciences, Middle East Technical University, 06800, Ankara, Turkey. mgursel@metu.edu.tr.
authors.forename: Mayda
authors.initials: M
authors.lastname: Gursel
authors.affiliation: THORLAB-Therapeutic Oligonucleotide Research Laboratory, Department of Molecular Biology and Genetics, Bilkent University, Bilkent, 06800, Ankara, Turkey.
authors.forename: Ihsan
authors.initials: I
authors.lastname: Gursel
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry* / Q000494:pharmacology*; D000595:Amino Acid Sequence; D001483:Base Sequence; D018899:CpG Islands; D004339:Drug Compounding / Q000379:methods*; D004355:Drug Stability; D004587:Electrophoresis, Agar Gel; D006801:Humans; D016898:Interferon-alpha / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000276:immunology*; D010455:Peptides / Q000737:chemistry
chemical_list: D000276:Adjuvants, Immunologic; D016898:Interferon-alpha; D008081:Liposomes; D009838:Oligodeoxyribonucleotides; D010455:Peptides
keywords: Cationic peptides; CpG ODN; Immune response; Liposomes; TLR9; Vaccine adjuvant