title: Comparison of Enzyme-Linked Immunosorbent Assay, Surface Plasmon Resonance and Biolayer Interferometry for Screening of Deoxynivalenol in Wheat and Wheat Dust.
abstract: A sample preparation method was developed for the screening of deoxynivalenol (DON) in wheat and wheat dust. Extraction was carried out with water and was successful due to the polar character of DON. For detection, an enzyme-linked immunosorbent assay (ELISA) was compared to the sensor-based techniques of surface plasmon resonance (SPR) and biolayer interferometry (BLI) in terms of sensitivity, affinity and matrix effect. The matrix effects from wheat and wheat dust using SPR were too high to further use this screenings method. The preferred ELISA and BLI methods were validated according to the criteria established in Commission Regulation 519/2014/EC and Commission Decision 2002/657/EC. A small survey was executed on 16 wheat lots and their corresponding dust samples using the validated ELISA method. A linear correlation (r = 0.889) was found for the DON concentration in dust versus the DON concentration in wheat (LOD wheat: 233 μg/kg, LOD wheat dust: 458 μg/kg).
authors.affiliation: Mycotoxin Prevention and Applied Microbiology Research Unit, United States Department of Agriculture-Agricultural Research Service-National Center for Agricultural Utilization Research (USDA-ARS-NCAUR), 1815 N. University Street, Peoria, IL 61604, USA. melanie.sanders@ars.usda.gov.
authors.forename: Melanie
authors.initials: M
authors.lastname: Sanders
authors.affiliation: School of Biotechnology, National Centre for Sensor Research and Biomedical Diagnostic Institute, Dublin City University, Dublin 9, Ireland. daniel.mcpartlin2@mail.dcu.ie.
authors.forename: Daniel
authors.initials: D
authors.lastname: McPartlin
authors.affiliation: School of Biotechnology, National Centre for Sensor Research and Biomedical Diagnostic Institute, Dublin City University, Dublin 9, Ireland. kara.moran24@mail.dcu.ie.
authors.forename: Kara
authors.initials: K
authors.lastname: Moran
authors.affiliation: Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310029, China. yirongguo@zju.edu.cn.
authors.forename: Yirong
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Applied Biosciences, Faculty of Bioscience Engineering, Ghent University, Valentin Vaerwyckweg 1, 9000 Ghent, Belgium. mia.eeckhout@ugent.be.
authors.forename: Mia
authors.initials: M
authors.lastname: Eeckhout
authors.affiliation: School of Biotechnology, National Centre for Sensor Research and Biomedical Diagnostic Institute, Dublin City University, Dublin 9, Ireland. richard.okennedy@dcu.ie.
authors.forename: Richard
authors.initials: R
authors.lastname: O'Kennedy
authors.affiliation: Department of Bioanalysis, Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium. sarah.desaeger@ugent.be.
authors.forename: Sarah
authors.initials: S
authors.lastname: De Saeger
authors.affiliation: Mycotoxin Prevention and Applied Microbiology Research Unit, United States Department of Agriculture-Agricultural Research Service-National Center for Agricultural Utilization Research (USDA-ARS-NCAUR), 1815 N. University Street, Peoria, IL 61604, USA. chris.maragos@ars.usda.gov.
authors.forename: Chris
authors.initials: C
authors.lastname: Maragos
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D004391:Dust / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D007368:Interferometry; D015203:Reproducibility of Results; D020349:Surface Plasmon Resonance; D014255:Trichothecenes / Q000032:analysis* / Q000276:immunology; D014908:Triticum / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D004391:Dust; D014255:Trichothecenes; C007262:deoxynivalenol
keywords: deoxynivalenol; immunosensor; monoclonal antibodies; wheat; wheat dust