title: Concentrations of tylvalosin and 3-O-acetyltylosin attained in the synovial fluid of swine after administration by oral gavage at 50 and 5 mg/kg.
abstract: The objectives of this study were to determine the concentration of tylvalosin (TVN) and its metabolite, 3-O-acetyltylosin (3AT) in the synovial fluid of growing pigs when administered as a single bolus by oral gavage at target doses of 50 mg/kg (Trial 1) and 5 mg/kg (Trial 2). TVN is a water soluble macrolide antimicrobial used in swine production. The stability of the drug in synovial fluid samples stored at -70 °C up to 28 days was also evaluated in Trial 2. In Trial 1, eight pigs were randomly assigned to one of eight time points for euthanasia and synovial fluid collection: 0, 1, 2, 3, 4, 6, 9, 12 h postgavage. For Trial 2, 24 pigs were randomly allocated to one terminal collection time point at 0, 2, 4, 6, 8 or 10 h postgavage. Synovial fluid was analyzed to determine TVN and 3AT concentrations. TVN and 3AT were detected in Trial 1 at all time points, except 0 h. At 2 h postgavage for trial 2, the mean concentrations peaked at 31.17 ng/mL (95% CI: 18.62-52.16) for TVN and at 58.82 ng/mL (95% CI: 35.14-98.46) for 3AT. Storage duration did not impact TVN or 3AT concentrations (P-value 0.9732).
authors.affiliation: Swine Medicine Education Center, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
authors.forename: P
authors.initials: P
authors.lastname: Canning
authors.affiliation: Swine Medicine Education Center, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
authors.forename: J
authors.initials: J
authors.lastname: Bates
authors.affiliation: Swine Medicine Education Center, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
authors.forename: K
authors.initials: K
authors.lastname: Hammen
authors.affiliation: Pharmacology Analytical Support Team, Veterinary Diagnostic Laboratory, Iowa State University, Ames, IA, USA.
authors.forename: J
authors.initials: J
authors.lastname: Coetzee
authors.affiliation: Pharmacology Analytical Support Team, Veterinary Diagnostic Laboratory, Iowa State University, Ames, IA, USA.
authors.forename: L
authors.initials: L
authors.lastname: Wulf
authors.affiliation: Pharmacology Analytical Support Team, Veterinary Diagnostic Laboratory, Iowa State University, Ames, IA, USA.
authors.forename: S
authors.initials: S
authors.lastname: Rajewski
authors.affiliation: Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
authors.forename: C
authors.initials: C
authors.lastname: Wang
authors.affiliation: Swine Medicine Education Center, College of Veterinary Medicine, Iowa State University, Ames, IA, USA. karriker@iastate.edu.
authors.forename: L
authors.initials: L
authors.lastname: Karriker
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics; D004305:Dose-Response Relationship, Drug; D006207:Half-Life; D013048:Specimen Handling; D013552:Swine / Q000378:metabolism*; D013582:Synovial Fluid / Q000737:chemistry*; D013997:Time Factors; D015645:Tylosin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics
chemical_list: C000621165:3-O-acetyltylosin; D000900:Anti-Bacterial Agents; C545430:tylvalosin; D015645:Tylosin