title: A comparative spectroscopic and calorimetric investigation of the interaction of amsacrine with heme proteins, hemoglobin and myoglobin.
abstract: The binding of the anilido aminoacridine derivative amsacrine with the heme proteins, hemoglobin, and myoglobin, was characterized by various spectroscopic and calorimetric methods. The binding affinity to hemoglobin was (1.21 ± .05) × 105M-1, while that to myoglobin was three times higher (3.59 ± .15) × 105M-1. The temperature-dependent fluorescence study confirmed the formation of ground-state complexes with both the proteins. The stronger binding to myoglobin was confirmed from both spectroscopic and calorimetric studies. The binding was exothermic in both cases at the three temperatures studied, and was favored by both enthalpy and entropy changes. Circular dichroism results, three-dimensional (3D) and synchronous fluorescence studies confirmed that the binding of amsacrine significantly changed the secondary structure of hemoglobin, while the change in the secondary structure of myoglobin was much less. New insights, in terms of structural and energetic aspects of the interaction of amsacrine with the heme proteins, presented here may help in understanding the structure-activity relationship, therapeutic efficacy, and drug design aspects of acridines.
authors.affiliation: a Biophysical Chemistry Laboratory , CSIR-Indian Institute of Chemical Biology , Kolkata 700 032 , India.
authors.forename: Debipreeta
authors.initials: D
authors.lastname: Bhowmik
authors.affiliation: a Biophysical Chemistry Laboratory , CSIR-Indian Institute of Chemical Biology , Kolkata 700 032 , India.
authors.forename: Gopinatha
authors.initials: G
authors.lastname: Suresh Kumar
mesh_terms: D000677:Amsacrine / Q000737:chemistry* / Q000378:metabolism; D002151:Calorimetry / Q000379:methods; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008024:Ligands; D015394:Molecular Structure; D009211:Myoglobin / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D013057:Spectrum Analysis / Q000379:methods; D013816:Thermodynamics
chemical_list: D006454:Hemoglobins; D008024:Ligands; D009211:Myoglobin; D000677:Amsacrine
keywords: calorimetry; fluorescence; hemoglobin; ligand amsacrine; myoglobin