title: Using MHC Molecules to Define a Chlamydia T Cell Vaccine.
abstract: Vaccines based on humoral immunity alone are unlikely to protect against infections caused by intracellular pathogens and today's most pressing infectious diseases of public health importance are caused by intracellular infections that include tuberculosis, malaria, HIV/AIDS, and others such as Chlamydia trachomatis. For these infections, vaccines that induce cellular immune responses are essential. Major impediments in developing such vaccines include difficulty in identifying relevant T cell antigens and delivering them in ways that elicit protective cellular immunity. Genomics and proteomics now provide tools to allow unbiased empirical identification of candidate T cell antigens. This approach represents an advance on bioinformatic searches for candidate T cell antigens. This chapter discusses an immunoproteomic approach we have used to identify Chlamydia T cell antigens. We further discuss how these T cell antigens can be developed into a human vaccine.
authors.affiliation: Vaccine Research Laboratory, UBC Centre for Disease Control, University of British Columbia, 655 West 12th Avenue, Vancouver, BC, Canada, V5Z 4R4.
authors.forename: Karuna P
authors.initials: KP
authors.lastname: Karunakaran
authors.affiliation: Vaccine Research Laboratory, UBC Centre for Disease Control, University of British Columbia, 655 West 12th Avenue, Vancouver, BC, Canada, V5Z 4R4.
authors.forename: Hong
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Biochemistry and Molecular Biology, Centre for High-Throughput Biology, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Leonard J
authors.initials: LJ
authors.lastname: Foster
authors.affiliation: Vaccine Research Laboratory, UBC Centre for Disease Control, University of British Columbia, 655 West 12th Avenue, Vancouver, BC, Canada, V5Z 4R4. robert.brunham@bccdc.ca.
authors.forename: Robert C
authors.initials: RC
authors.lastname: Brunham
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D001854:Bone Marrow Cells / Q000166:cytology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002692:Chlamydia trachomatis / Q000276:immunology*; D003001:Cloning, Molecular; D019295:Computational Biology; D003713:Dendritic Cells / Q000166:cytology; D005260:Female; D006649:Histocompatibility Antigens / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D007114:Immunization; D051379:Mice; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000166:cytology / Q000378:metabolism; D040901:Proteomics; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D012475:Salmonella / Q000166:cytology / Q000378:metabolism
chemical_list: D001428:Bacterial Vaccines; D006649:Histocompatibility Antigens; D011994:Recombinant Proteins
keywords: Antigen; Chlamydia; Dendritic cells; Immunoproteomics; MHC; T cell; Vaccine