title: Enhancement of Fluorescent Probe Penetration into Tumors In Vivo Using Unseeded Inertial Cavitation.
abstract: Ultrasound-induced cavitation has found many applications in the field of cancer therapy. One of its beneficial effects is the enhancement of drug intake by tumor cells. Our group has developed a device that can create and control unseeded cavitation in tissue using ultrasound. We conducted experiments on tumor-bearing mice using our device to assess the impact of sonication on the penetration of fluorescent probes into tumor cells. We studied the influence of pressure level, timing of sonication and sonication duration on treatment efficiency. Our results indicate that fluorescent probes penetrate better into tumors exposed to ultrasound. The best results revealed an increase in penetration of 61% and were obtained when sonicating the tumor in presence of the probes with a peak negative pressure at focus of 19 MPa. At this pressure level, the treatment generated only minor skin damage. Treatments could be significantly accelerated as equivalent enhanced penetration of probes was achieved when multiplying the initial raster scan speed by a factor of four.
authors.affiliation: Inserm, U1032, LabTau, Lyon, France; Université de Lyon, Lyon, France. Electronic address: fabriceprieur@yahoo.com.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Prieur
authors.affiliation: Centre de Recherche en Oncologie Expérimentale, Institut de Recherche Pierre Fabre, Toulouse, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Pillon
authors.affiliation: Inserm, U1032, LabTau, Lyon, France; Université de Lyon, Lyon, France; Caviskills SAS, Vaulx-en-Velin, France.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Mestas
authors.affiliation: Centre de Recherche en Oncologie Expérimentale, Institut de Recherche Pierre Fabre, Toulouse, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Cartron
authors.affiliation: Centre de Recherche en Oncologie Expérimentale, Institut de Recherche Pierre Fabre, Toulouse, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Cèbe
authors.affiliation: Centre de Recherche en Oncologie Expérimentale, Institut de Recherche Pierre Fabre, Toulouse, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Chansard
authors.affiliation: Inserm, U1032, LabTau, Lyon, France; Université de Lyon, Lyon, France.
authors.forename: Maxime
authors.initials: M
authors.lastname: Lafond
authors.affiliation: Inserm, U1032, LabTau, Lyon, France; Université de Lyon, Lyon, France; Caviskills SAS, Vaulx-en-Velin, France.
authors.forename: Cyril
authors.initials: C
authors.lastname: Lafon
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004867:Equipment Design; D005260:Female; D005456:Fluorescent Dyes / Q000493:pharmacokinetics*; D006801:Humans; D008546:Melanoma, Experimental / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D000069453:Ultrasonic Waves
chemical_list: D005456:Fluorescent Dyes
keywords: Cavitation; Fluorescence; Melanoma; Probe; Sonoporation