title: RTN3 Regulates the Expression Level of Chemokine Receptor CXCR4 and is Required for Migration of Primordial Germ Cells.
abstract: CXCR4 is a crucial chemokine receptor that plays key roles in primordial germ cell (PGC) homing. To further characterize the CXCR4-mediated migration of PGCs, we screened CXCR4-interacting proteins using yeast two-hybrid screening. We identified reticulon3 (RTN3), a member of the reticulon family, and considered an apoptotic signal transducer, as able to interact directly with CXCR4. Furthermore, we discovered that the mRNA and protein expression levels of CXCR4 could be regulated by RTN3. We also found that RTN3 altered CXCR4 translocation and localization. Moreover, increasing the signaling of either CXCR4b or RTN3 produced similar PGC mislocalization phenotypes in zebrafish. These results suggested that RTN3 modulates PGC migration through interaction with, and regulation of, CXCR4.
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuan Ming Yuan West Road, Beijing 100193, China. lihaitao_cau@163.com.
authors.forename: Haitao
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuan Ming Yuan West Road, Beijing 100193, China. lr_@cau.edu.cn.
authors.forename: Rong
authors.initials: R
authors.lastname: Liang
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuan Ming Yuan West Road, Beijing 100193, China. nan0914@126.com.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Lu
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuan Ming Yuan West Road, Beijing 100193, China. wmx_arctic@cau.edu.cn.
authors.forename: Mengxia
authors.initials: M
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuan Ming Yuan West Road, Beijing 100193, China. lzdws@cau.edu.cn.
authors.forename: Zandong
authors.initials: Z
authors.lastname: Li
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000032:analysis / Q000378:metabolism*; D002465:Cell Movement; D005854:Germ Cells / Q000166:cytology* / Q000378:metabolism; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000378:metabolism*; D000072417:Protein Domains; D060066:Protein Interaction Maps; D019718:Receptors, CXCR4 / Q000032:analysis / Q000378:metabolism*; D015027:Zebrafish
chemical_list: C527276:CXCR4 protein, human; D002352:Carrier Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C476198:RTN3 protein, human; D019718:Receptors, CXCR4
keywords: CXCR4; RTN3; primordial germ cells