title: M1 macrophage infiltrations and histological changes in the liver after portal vein embolization using fibrinogen and OK432 in the rat.
abstract: The mechanism of anti-tumor effect of transarterial Immuno-Embolization (TIE) using OK-432 has not been well elucidated. In this study, we aimed to investigate the tissue injury and immune response after portal venous embolization (PVE) with/without OK-432. Embolic materials (L group: lipiodol, LF group: lipiodol+fibrinogen, LO group: lipiodol+OK-432, LFO group: lipiodol+fibrinogen+OK-432) were administered via the right portal vein in Wistar rats. The histological findings in LFO group demonstrated liver damage with severe architectural changes. The concentrations of CD68(+) cells were observed in a time-dependent manner; it was significantly increased in the LO group on day 1 and in the LFO group on day 3. CD68(+)CD163(-) macrophages significantly increased in the LFO group on day 7 (P<0.05). In conclusion, PVE with fibrinogen and OK-432 markedly increased the CD68(+)CD163(-) infiltrating macrophages around the peri-portal area in the liver. This novel technique could be applied as immune-enhanced chemo-embolization of liver tumors.
authors.affiliation: Department of Regenerative Surgery, Fukushima Medical University, Fukushima, Japan.
authors.forename: Tetsu
authors.initials: T
authors.lastname: Sato
authors.affiliation: Department of Regenerative Surgery, Fukushima Medical University, Fukushima, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Marubashi
authors.affiliation: Department of Regenerative Surgery, Fukushima Medical University, Fukushima, Japan. Electronic address: a-kenjo@fmu.ac.jp.
authors.forename: Akira
authors.initials: A
authors.lastname: Kenjo
authors.affiliation: Department of Regenerative Surgery, Fukushima Medical University, Fukushima, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Tsuchiya
authors.affiliation: Department of Regenerative Surgery, Fukushima Medical University, Fukushima, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kimura
authors.affiliation: Department of Regenerative Surgery, Fukushima Medical University, Fukushima, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Sato
authors.affiliation: Department of Regenerative Surgery, Fukushima Medical University, Fukushima, Japan.
authors.forename: Junichiro
authors.initials: J
authors.lastname: Watanabe
authors.affiliation: Department of Diagnostic Pathology, Fukushima Medical University, Fukushima, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Tasaki
authors.affiliation: Department of Diagnostic Pathology, Fukushima Medical University, Fukushima, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Hashimoto
authors.affiliation: Department of Cell Science, Institute of Biomedical Sciences, Fukushima Medical University, Fukushima, Japan.
authors.forename: Ikuo
authors.initials: I
authors.lastname: Wada
authors.affiliation: Department of Regenerative Surgery, Fukushima Medical University, Fukushima, Japan.
authors.forename: Mitsukazu
authors.initials: M
authors.lastname: Gotoh
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects; D004621:Embolization, Therapeutic; D005340:Fibrinogen / Q000008:administration & dosage*; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008113:Liver Neoplasms / Q000628:therapy*; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D008297:Male; D010844:Picibanil / Q000008:administration & dosage*; D011169:Portal Vein / Q000187:drug effects* / Q000601:surgery; D051381:Rats; D017208:Rats, Wistar; D011956:Receptors, Cell Surface / Q000378:metabolism; D000098902:CD163 Antigen; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D005340:Fibrinogen; D010844:Picibanil; D011956:Receptors, Cell Surface; D000098902:CD163 Antigen; C067980:CD68 antigen, human; D000097382:CD68 Molecule
keywords: Hepatocellular carcinoma; Immune response; M1 macrophage; T helper 1 (Th1) cell