title: Organism-wide studies into pathogenicity and morphogenesis in Talaromyces marneffei.
abstract: Organism-wide approaches examining the genetic mechanisms controlling growth and proliferation have proven to be a powerful tool in the study of pathogenic fungi. For many fungal pathogens techniques to study transcription and protein expression are particularly useful, and offer insights into infection processes by these species. Here we discuss the use of approaches such as differential display, suppression subtractive hybridization, microarray, RNA-seq, proteomics, genetic manipulation and infection models for the AIDS-defining pathogen Talaromyces marneffei. Together these methods have broadened our understanding of the biological processes, and genes that underlie them, which are involved in switching between the saprophytic and pathogenic states of T. marneffei, the maintenance of these two specialized cell types and its ability to cause disease.
authors.affiliation: Genetics, Genomics & Development, School of BioSciences, University of Melbourne, Victoria 3010, Australia.
authors.forename: Harshini
authors.initials: H
authors.lastname: Weerasinghe
authors.affiliation: Genetics, Genomics & Development, School of BioSciences, University of Melbourne, Victoria 3010, Australia.
authors.forename: Michael
authors.initials: M
authors.lastname: Payne
authors.affiliation: Genetics, Genomics & Development, School of BioSciences, University of Melbourne, Victoria 3010, Australia.
authors.forename: Sally
authors.initials: S
authors.lastname: Beard
authors.affiliation: Genetics, Genomics & Development, School of BioSciences, University of Melbourne, Victoria 3010, Australia.
authors.forename: Alex
authors.initials: A
authors.lastname: Andrianopoulos
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D009181:Mycoses / Q000382:microbiology* / Q000473:pathology*; D032901:Talaromyces / Q000166:cytology* / Q000235:genetics / Q000472:pathogenicity*
keywords: Penicillum marneffei; Talaromyces marneffei; genetic manipulation tools; infection models; morphogenesis; pathogenesis; proteomics; transcriptomics