title: DNA Vaccines: A Strategy for Developing Novel Multivalent TB Vaccines.
abstract: Multivalent DNA vaccines that are delivered by electroporation (EP) through muscle tissue provide a novel method for eliciting immunity against tuberculosis (TB) as well as a broad range of diseases including HIV and cancers. Proper plasmid construction containing suitable protective TB antigens capable of evoking desired vaccine-induced responses would lead to the appropriate induction of both humoral and cellular immunity. DNA vaccines are safe and of low cost in comparison to traditional vaccines while also providing potentially effective prophylactic or therapeutic modalities against currently untreatable diseases. Here, we describe the steps for developing a rational multivalent TB DNA vaccine delivered with intramuscular EP in mice.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Pennsylvania, 505 Stellar-Chance Laboratories, 422 Curie Boulevard, Philadelphia, PA, 19104, USA.
authors.forename: Jaemi S
authors.initials: JS
authors.lastname: Chu
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Pennsylvania, 505 Stellar-Chance Laboratories, 422 Curie Boulevard, Philadelphia, PA, 19104, USA.
authors.forename: Daniel O
authors.initials: DO
authors.lastname: Villarreal
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Pennsylvania, 505 Stellar-Chance Laboratories, 422 Curie Boulevard, Philadelphia, PA, 19104, USA. dbweiner@mail.med.upenn.edu.
authors.forename: David B
authors.initials: DB
authors.lastname: Weiner
mesh_terms: D000818:Animals; D006801:Humans; D014376:Tuberculosis / Q000276:immunology / Q000517:prevention & control; D032581:Tuberculosis Vaccines / Q000191:economics / Q000276:immunology*; D019444:Vaccines, DNA / Q000191:economics / Q000276:immunology*
chemical_list: D032581:Tuberculosis Vaccines; D019444:Vaccines, DNA
keywords: Antigens; DNA vaccines; Electroporation; Intramuscular; Multivalent vaccines; Mycobacteria tuberculosis; TB; Tuberculosis