title: Corynebacterium faecale sp. nov., isolated from the faeces of Assamese macaque.
abstract: A Gram-stain-positive, facultatively anaerobic, short rod-shaped, oxidase-negative and non-motile novel strain, designated YIM 101505T, was isolated from the faeces of a primate, Assamese macaque, and was studied to determine its taxonomic position. The cell wall contained meso-diaminopimelic acid and short-chain mycolic acids. Whole cell sugars were mannose, galactose and arabinose as major components. The major fatty acids (>10 %) were C18 : 1ω9c, C16 : 0 and C17 : 1ω8c and the major menaquinone was MK-9(H2). The polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, glycolipid and six unidentified lipids. The new isolate shared most of the typical chemotaxonomic characteristics of members of the genus Corynebacterium. The closest related species was Corynebacterium efficiens based on 16S rRNA gene (98.1 % similarity) and partial rpoB gene (91.4 % similarity) sequences. Similarities with other species of this genus were below 97 % based on the 16S rRNA gene. The DNA-DNA hybridization value between YIM 101505T and C. efficiens DSM 44549T was 47.7±3.6 %. Moreover, the physiological and biochemical characteristics of YIM 101505T and C. efficiens DSM 44549T were different. Thus, strain YIM 101505T is considered to represent a novel member of the genus Corynebacterium, for which the name Corynebacterium faecale sp. nov. is proposed. The type strain is YIM 101505T (=DSM 45971T=CCTCC AB 2013226T).
authors.affiliation: Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University, Shenyang 110819, PR China.
authors.forename: Xiu
authors.initials: X
authors.lastname: Chen
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Gui-Ding
authors.initials: GD
authors.lastname: Li
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Qin-Yuan
authors.initials: QY
authors.lastname: Li
authors.affiliation: Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University, Shenyang 110819, PR China.
authors.forename: Cai-Juan
authors.initials: CJ
authors.lastname: Hu
authors.affiliation: Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University, Shenyang 110819, PR China.
authors.forename: Cheng-Bin
authors.initials: CB
authors.lastname: Liu
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Cheng-Lin
authors.initials: CL
authors.lastname: Jiang
authors.affiliation: Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University, Shenyang 110819, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Han
authors.affiliation: Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University, Shenyang 110819, PR China.
authors.forename: Xue-Shi
authors.initials: XS
authors.lastname: Huang
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002681:China; D003352:Corynebacterium / Q000737:chemistry / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D008251:Macaca / Q000382:microbiology*; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D010743:Phospholipids