title: Physiological effects of major up-regulated Alnus glutinosa peptides on Frankia sp. ACN14a.
abstract: Alnus glutinosa has been shown previously to synthesize, in response to nodulation by Frankia sp. ACN14a, an array of peptides called Alnus symbiotic up-regulated peptides (ASUPs). In a previous study one peptide (Ag5) was shown to bind to Frankia nitrogen-fixing vesicles and to modify their porosity. Here we analyse four other ASUPs, alongside Ag5, to determine whether they have different physiological effects on in vitro grown Frankia sp. ACN14a. The five studied peptides were shown to have different effects on nitrogen fixation, respiration, growth, the release of ions and amino acids, as well as on cell clumping and cell lysis. The mRNA abundance for all five peptides was quantified in symbiotic nodules and one (Ag11) was found to be more abundant in the meristem part of the nodule. These findings point to some peptides having complementary effects on Frankia cells.
authors.affiliation: Université Lyon 1, Université de Lyon, CNRS, Ecologie Microbienne, UMR 5557, Villeurbanne, 69622 Cedex, France.
authors.forename: Lorena
authors.initials: L
authors.lastname: Carro
authors.affiliation: Université Lyon 1, Université de Lyon, CNRS, Ecologie Microbienne, UMR 5557, Villeurbanne, 69622 Cedex, France.
authors.forename: Petar
authors.initials: P
authors.lastname: Pujic
authors.affiliation: Université Lyon 1, Université de Lyon, CNRS, Ecologie Microbienne, UMR 5557, Villeurbanne, 69622 Cedex, France.
authors.forename: Nicole
authors.initials: N
authors.lastname: Alloisio
authors.affiliation: Université Lyon 1, Université de Lyon, CNRS, Ecologie Microbienne, UMR 5557, Villeurbanne, 69622 Cedex, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Fournier
authors.affiliation: Université Lyon 1, Université de Lyon, CNRS, Ecologie Microbienne, UMR 5557, Villeurbanne, 69622 Cedex, France.
authors.forename: Hasna
authors.initials: H
authors.lastname: Boubakri
authors.affiliation: Université Lyon 1, Université de Lyon, CNRS, Ecologie Microbienne, UMR 5557, Villeurbanne, 69622 Cedex, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Poly
authors.affiliation: Université de Lyon, INSA Lyon, INRA, UMR203 BF2I, Biologie Fonctionnelle Insectes et Interactions, Villeurbanne, 69622 Cedex, France.
authors.forename: Marjolaine
authors.initials: M
authors.lastname: Rey
authors.affiliation: Université de Lyon, INSA Lyon, INRA, UMR203 BF2I, Biologie Fonctionnelle Insectes et Interactions, Villeurbanne, 69622 Cedex, France.
authors.forename: Abdelaziz
authors.initials: A
authors.lastname: Heddi
authors.affiliation: Université Lyon 1, Université de Lyon, CNRS, Ecologie Microbienne, UMR 5557, Villeurbanne, 69622 Cedex, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Normand
mesh_terms: D029661:Alnus / Q000378:metabolism*; D001422:Bacterial Adhesion / Q000187:drug effects*; D023082:Defensins / Q000494:pharmacology*; D040161:Frankia / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D009586:Nitrogen Fixation / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects*; D010940:Plant Proteins / Q000494:pharmacology*; D055170:Plant Root Nodulation / Q000502:physiology; D018517:Plant Roots / Q000378:metabolism / Q000382:microbiology; D012988:Soil Microbiology; D013559:Symbiosis / Q000187:drug effects*
chemical_list: D023082:Defensins; D010940:Plant Proteins