title: Structural basis of the interaction between the meningitis pathogen Streptococcus suis adhesin Fhb and its human receptor.
abstract: The recently identified Streptococcus suis adhesin factor H-binding protein (Fhb) targets the host cellular receptor glycolipid GbO3 through its N terminus. However, it is unclear how Fhb interacts with its receptor. Here, we determined the complex structure of factor H-binding protein receptor-binding domain (Fhb RBD) with Gb2, an analog of its receptor, revealing that Gb2 binds in a pocket of the β sandwich core domain. We identified the key residues for Fhb RBD receptor binding using mutagenesis and isothermal titration calorimetry. Mutagenesis analyses indicated that Fhb binds to Gb2 mainly through hydrogen and hydrophobic interactions. Our findings provided structural insights into the Fhb-mediated host-pathogen interactions of S. suis.
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, China.
authors.forename: Chunmao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, China.
authors.forename: Huaijie
authors.initials: H
authors.lastname: Hao
authors.affiliation: Key Laboratory for Protein Sciences of Ministry of Education, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China.
authors.forename: You
authors.initials: Y
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, China.
authors.forename: Decong
authors.initials: D
authors.lastname: Kong
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, China.
authors.forename: Shaolong
authors.initials: S
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, China.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Key Laboratory for Protein Sciences of Ministry of Education, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China.
authors.forename: Maojun
authors.initials: M
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, China.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D006801:Humans; D017354:Point Mutation; D011485:Protein Binding; D017011:Streptococcus suis / Q000737:chemistry* / Q000472:pathogenicity; D014281:Trihexosylceramides / Q000737:chemistry / Q000378:metabolism*
chemical_list: D018829:Adhesins, Bacterial; D014281:Trihexosylceramides; C018549:globotriaosylceramide
keywords: Fhb; Streptococcus suis; hydrogen; hydrophobic interactions; pathogen and host interaction; protein and disaccharide complex