title: Droplet digital PCR and qRT-PCR to detect circulating miR-21 in laryngeal squamous cell carcinoma and pre-malignant laryngeal lesions.
abstract: CONCLUSIONS
  The present study indicates that miR-21 is involved in progression from normal to pre-malignant laryngeal lesions (PLLs) and from PLLs to laryngeal squamous cell carcinoma (LSCC). Furthermore, normalized PCR results for miR-21 might be used to discriminate between normal and ordinary hyperplasia before the emergence of dysplasias and pre-malignant lesions with malignant potential.
  
  
  OBJECTIVE
  To investigate a sensitive marker that contributes to progression from normal tissue to PLLs and from PLLs to LSCC.
  
  
  METHODS
  In 116 PLLs and LSCC patients and 19 without dysplasia matched sets of tissue and plasma samples from Beijing Tongren Hospital, miR-21 was analysed by droplet digital PCR and quantitative real-time polymerase chain reaction based on paraffin-embedded tumour tissue and plasma.
  
  
  RESULTS
  Compared with controls, miR-21 levels in tissue and plasma were significantly higher for both PLL and LSCC groups (for both groups vs controls: p < 0.0001 for tissue; p < 0.05 for plasma). Areas under receiver-operating curves (AUC) for tissue miR-21 were PLL group vs controls: 0.9026 and LSCC group vs controls: 0.8681. For plasma miR-21, AUCs were PLL group vs controls: 0.7355 and LSCC group vs control: 0.6723.
authors.affiliation: a Department of Pathology , Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology , Beijing , PR China ;
authors.forename: Li
authors.initials: L
authors.lastname: Wei
authors.affiliation: a Department of Pathology , Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology , Beijing , PR China ;|b Beijing Tsinghua Changgung Hospital , Beijing , PR China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Mao
authors.affiliation: a Department of Pathology , Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology , Beijing , PR China ;
authors.forename: Honggang
authors.initials: H
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000175:diagnosis / Q000473:pathology; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000473:pathology; D007830:Larynx / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D011230:Precancerous Conditions / Q000097:blood* / Q000175:diagnosis / Q000473:pathology; D011446:Prospective Studies; D012372:ROC Curve; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; C524940:MIRN21 microRNA, human; D035683:MicroRNAs
keywords: Pre-malignant laryngeal lesion; droplet digital PCR; laryngeal squamous cell carcinoma; miRNA-21