title: High-throughput determination of multi-mycotoxins in Chinese yam and related products by ultra fast liquid chromatography coupled with tandem mass spectrometry after one-step extraction.
abstract: A simple, accurate and sensitive ultra fast liquid chromatography coupled with tandem mass spectrometry (UFLC-MS/MS) method was developed for high-throughput determination of aflatoxins (AFB1, AFB2, AFG1 and AFG2), ochratoxin A (OTA), fumonisins (FB1 and FB2) and zearalenone (ZEA) in Chinese yam, yam flours and yam-derived products. Mycotoxins were extracted from the samples with methanol-water-formic acid (79:20:1, v/v/v) and no further cleanup step before analysis. After optimization of some crucial parameters including sample preparation, chromatographic separation and MS/MS conditions, the method was successfully validated to exhibit excellent performance in terms of satisfactory linearity (r≥0.9977), limits of detection (≤0.15ngmL(-1)) and quantification (≤0.5ngmL(-1)) with good precision (RSD for intra- and inter-day variations of ≤4.65% and 6.31%, respectively), good accuracy (recoveries of 71.0-106.0%) and robustness, together with short run time (8min/sample). The developed method was applied for simultaneous detection and quantification of the above 8 mycotaxins in 27 batches of Chinese yam and related products collected from different markets and pharmacies in China. The results revealed that 1 normal sample and 4 moldy samples were found to be contaminated with different mycotoxins. The detected concentrations of AFB1 in 2 moldy samples exceeded the regulatory maximum residue levels. The proposed method was capable for simultaneous determination of mycotoxins in this and other types of complex matrices.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China; School of Pharmacy, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Menghua
authors.initials: M
authors.lastname: Li
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Kong
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China; College of Traditional Chinese Medicine, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Liu
authors.affiliation: Pharmacy College, Liaoning Medical University, Jinzhou, Liaoning 121001, China.
authors.forename: Qiutao
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Dou
authors.affiliation: School of Pharmacy, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Ou-Yang
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China. Electronic address: yangmeihua15@hotmail.com.
authors.forename: Meihua
authors.initials: M
authors.lastname: Yang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D027742:Dioscorea / Q000737:chemistry*; D057166:High-Throughput Screening Assays / Q000379:methods; D057230:Limit of Detection; D016014:Linear Models; D009183:Mycotoxins / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009183:Mycotoxins; D010936:Plant Extracts
keywords: Chinese yam and yam flours; High-throughput determination; Multi-mycotoxins; One-step extraction; UFLC–MS/MS; Yam-derived products