title: Redox Proteomic Profiling of Specifically Carbonylated Proteins in the Serum of Triple Transgenic Alzheimer's Disease Mice.
abstract: Oxidative stress is a key event in the onset and progression of neurodegenerative diseases, including Alzheimer's disease (AD). To investigate the role of oxidative stress in AD and to search for potential biomarkers in peripheral blood, serums were collected in this study from the 3-, 6-, and 12-month-old triple transgenic AD mice (3×Tg-AD mice) and the age- and sex-matched non-transgenic (non-Tg) littermates. The serum oxidized proteins were quantified by slot-blot analysis and enzyme-linked immunosorbent assay (ELISA) to investigate the total levels of serum protein carbonyl groups. Western blotting, in conjunction with two-dimensional gel electrophoresis (2D-Oxyblot), was employed to identify and quantify the specifically-carbonylated proteins in the serum of 3×Tg-AD mice. The results showed that the levels of serum protein carbonyls were increased in the three month old 3×Tg-AD mice compared with the non-Tg control mice, whereas no significant differences were observed in the six and 12 months old AD mice, suggesting that oxidative stress is an early event in AD progression. With the application of 2D-Oxyblot analysis, (immunoglobin) Ig gamma-2B chain C region (IGH-3), Ig lambda-2 chain C region (IGLC2), Ig kappa chain C region (IGKC), and Ig kappa chain V-V region HP R16.7 were identified as significantly oxidized proteins compared with the control. Among them IGH-3 and IGKC were validated via immunoprecipitation and Western blot analysis. Identification of oxidized proteins in the serums of 3×Tg-AD mice can not only reveal potential roles of those proteins in the pathogenesis of AD but also provide potential biomarkers of AD at the early stage.
authors.affiliation: Shenzhen Key Laboratory of Marine Biotechnology and Ecology, College of Life Science, Shenzhen University, Shenzhen 518060, China. shenlim@163.com.
authors.forename: Liming
authors.initials: L
authors.lastname: Shen
authors.affiliation: Shenzhen Key Laboratory of Marine Biotechnology and Ecology, College of Life Science, Shenzhen University, Shenzhen 518060, China. qyzcyj1239329@163.com.
authors.forename: Youjiao
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Shenzhen Key Laboratory of Marine Biotechnology and Ecology, College of Life Science, Shenzhen University, Shenzhen 518060, China. aochuyang@163.com.
authors.forename: Aochu
authors.initials: A
authors.lastname: Yang
authors.affiliation: Shenzhen Key Laboratory of Marine Biotechnology and Ecology, College of Life Science, Shenzhen University, Shenzhen 518060, China. chenchengszu@163.com.
authors.forename: Cheng
authors.initials: C
authors.lastname: Chen
authors.affiliation: Shenzhen Key Laboratory of Marine Biotechnology and Ecology, College of Life Science, Shenzhen University, Shenzhen 518060, China. xiaoliping3@163.com.
authors.forename: Liping
authors.initials: L
authors.lastname: Liao
authors.affiliation: Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Science, Shenzhen University, Shenzhen 518060, China. shuimingli@szu.edu.cn.
authors.forename: Shuiming
authors.initials: S
authors.lastname: Li
authors.affiliation: Shenzhen Key Laboratory of Marine Biotechnology and Ecology, College of Life Science, Shenzhen University, Shenzhen 518060, China. yingming@szu.edu.cn.
authors.forename: Ming
authors.initials: M
authors.lastname: Ying
authors.affiliation: Shenzhen Key Laboratory of Marine Biotechnology and Ecology, College of Life Science, Shenzhen University, Shenzhen 518060, China. jtian@szu.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Tian
authors.affiliation: Shenzhen Key Laboratory of Marine Biotechnology and Ecology, College of Life Science, Shenzhen University, Shenzhen 518060, China. liuqiong@szu.edu.cn.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Shenzhen Key Laboratory of Marine Biotechnology and Ecology, College of Life Science, Shenzhen University, Shenzhen 518060, China. jzni@szu.edu.cn.
authors.forename: Jiazuan
authors.initials: J
authors.lastname: Ni
mesh_terms: D000544:Alzheimer Disease / Q000097:blood / Q000378:metabolism*; D000818:Animals; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D018384:Oxidative Stress; D050050:Protein Carbonylation; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
chemical_list: D020543:Proteome
keywords: Alzheimer’s disease; biomarker; oxidative stress; protein carbonylation; redox proteomics; serum; triple transgenic mice