title: Functional behavior of bio-electrochemical treatment system with increasing azo dye concentrations: Synergistic interactions of biocatalyst and electrode assembly.
abstract: Treatment of dye bearing wastewater through biological machinery is particularly challenging due to its recalcitrant and inhibitory nature. In this study, functional behavior and treatment efficiency of bio-electrochemical treatment (BET) system was evaluated with increasing azo dye concentrations (100, 200, 300 and 500mg dye/l). Maximum dye removal was observed at 300mg dye/l (75%) followed by 200mg dye/l (65%), 100mg dye/l (62%) and 500mg dye/l (58%). Concurrent increment in dye load resulted in enhanced azo reductase and dehydrogenase activities respectively (300mg dye/l: 39.6U; 4.96μg/ml). Derivatives of cyclic voltammograms also supported the involvement of various membrane bound redox shuttlers, viz., cytochrome-c, cytochrome-bc1 and flavoproteins during the electron transfer. Bacterial respiration during BET operation utilized various electron acceptors such as electrodes and dye intermediates with simultaneous bioelectricity generation. This study illustrates the synergistic interaction of biocatalyst with electrode assembly for efficient treatment of azo dye wastewater.
authors.affiliation: Bioengineering and Environmental Sciences (BEES), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India.
authors.forename: S
authors.initials: S
authors.lastname: Sreelatha
authors.affiliation: Bioengineering and Environmental Sciences (BEES), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India.
authors.forename: G
authors.initials: G
authors.lastname: Velvizhi
authors.affiliation: Bioengineering and Environmental Sciences (BEES), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India.
authors.forename: A
authors.initials: A
authors.lastname: Naresh Kumar
authors.affiliation: Bioengineering and Environmental Sciences (BEES), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India. Electronic address: vmohan_s@yahoo.com.
authors.forename: S
authors.initials: S
authors.lastname: Venkata Mohan
mesh_terms: D001391:Azo Compounds / Q000378:metabolism*; D057919:Biological Oxygen Demand Analysis; D004396:Coloring Agents / Q000378:metabolism; D004566:Electrodes; D004579:Electron Transport; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D010088:Oxidoreductases; D062065:Wastewater
keywords: Biocatalyst; Bioelectricity; Microbial fuel cell; Redox mediators; Solid electron acceptor