title: Production of a thermal stress resistant mutant Euglena gracilis strain using Fe-ion beam irradiation.
abstract: Euglena gracilis is a common phytoplankton species, which also has motile flagellate characteristics. Recent research and development has enabled the industrial use of E. gracilis and selective breeding of this species is expected to further expand its application. However, the production of E. gracilis nuclear mutants is difficult because of the robustness of its genome. To establish an efficient mutation induction procedure for E. gracilis, we employed Fe-ion beam irradiation in the RIKEN RI beam factory. A decrease in the survival rate was observed with the increase in irradiation dose, and the upper limit used for E. gracilis selective breeding was around 50 Gy. For a practical trial of Fe-ion irradiation, we conducted a screening to isolate high-temperature-tolerant mutants. The screening yielded mutants that proliferated faster than the wild-type strain at 32 °C. Our results demonstrate the effectiveness of heavy-ion irradiation on E. gracilis selective breeding.
authors.affiliation: a Euglena Co., Ltd. , Tokyo , Japan.|b JST , CREST , Tokyo , Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Yamada
authors.affiliation: c RIKEN Nishina Center for Accelerator-based Science , Saitama , Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kazama
authors.affiliation: a Euglena Co., Ltd. , Tokyo , Japan.
authors.forename: Sharbanee
authors.initials: S
authors.lastname: Mitra
authors.affiliation: a Euglena Co., Ltd. , Tokyo , Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Marukawa
authors.affiliation: a Euglena Co., Ltd. , Tokyo , Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Arashida
authors.affiliation: c RIKEN Nishina Center for Accelerator-based Science , Saitama , Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Abe
authors.affiliation: b JST , CREST , Tokyo , Japan.|d Shimane University , Shimane , Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ishikawa
authors.affiliation: a Euglena Co., Ltd. , Tokyo , Japan.|b JST , CREST , Tokyo , Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Suzuki
mesh_terms: D000222:Adaptation, Physiological; D004307:Dose-Response Relationship, Radiation; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D005056:Euglena gracilis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects*; D018503:Genome, Protozoan; D006358:Hot Temperature; D016296:Mutagenesis / Q000187:drug effects / Q000528:radiation effects*; D009153:Mutagens / Q000633:toxicity; D009154:Mutation; D010839:Phytoplankton / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects*; D011839:Radiation, Ionizing
chemical_list: D009153:Mutagens; D005020:Ethyl Methanesulfonate
keywords: Euglena gracilis; Fe-ion beam irradiation; high-temperature tolerance