title: Pittosporum cryptic virus 1: genome sequence completion using next-generation sequencing.
abstract: Next-generation sequencing (NGS) was applied to dsRNAs extracted from an Italian pittosporum plant infected with pittosporum cryptic virus 1 (PiCV1). NGS allowed assembly of the full genome sequence of PiCV1, comprising dsRNA1 (1.9 kbp) and dsRNA2 (1.5 kbp), which encode the RNA-dependent RNA polymerase and capsid protein genes, respectively. Phylogenetic and sequence analyses confirmed that PiCV1 is a new member of the genus Deltapartitivirus, family Partiviridae. From the same plant, NSG also permitted assembly of the complete genome sequence of eggplant mottled dwarf virus (EMDV), which shared 86 % to 98 % nucleotide sequence identity with complete and partial sequences (ca 6750 nt) of other known EMDV isolates with sequences available in the GenBank database.
authors.affiliation: Istituto Agronomico Mediterraneo di Bari, Via Ceglie 9, 70010, Valenzano (Bari), Italy. elbeaino@iamb.it.
authors.forename: Toufic
authors.initials: T
authors.lastname: Elbeaino
authors.affiliation: Istituto Agronomico Mediterraneo di Bari, Via Ceglie 9, 70010, Valenzano (Bari), Italy.
authors.forename: Raied Abou
authors.initials: RA
authors.lastname: Kubaa
authors.affiliation: Department of Plant Protection, Faculty of Agriculture, University of Çanakkale Onsekiz Mart, 17020, Canakkale, Turkey.
authors.forename: Hasan Tuna
authors.initials: HT
authors.lastname: Tuzlali
authors.affiliation: Istituto Agronomico Mediterraneo di Bari, Via Ceglie 9, 70010, Valenzano (Bari), Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Digiaro
mesh_terms: D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D012328:RNA Viruses / Q000145:classification / Q000235:genetics* / Q000276:immunology; D012367:RNA, Viral / Q000235:genetics
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral