title: High Conservation in Transcriptomic and Proteomic Response of White Sturgeon to Equipotent Concentrations of 2,3,7,8-TCDD, PCB 77, and Benzo[a]pyrene.
abstract: Adverse effects associated with exposure to dioxin-like compounds (DLCs) are mediated primarily through activation of the aryl hydrocarbon receptor (AHR). However, little is known about the cascades of events that link activation of the AHR to apical adverse effects. Therefore, this study used high-throughput, next-generation molecular tools to investigate similarities and differences in whole transcriptome and whole proteome responses to equipotent concentrations of three agonists of the AHR, 2,3,7,8-TCDD, PCB 77, and benzo[a]pyrene, in livers of a nonmodel fish, the white sturgeon (Acipenser transmontanus). A total of 926 and 658 unique transcripts were up- and down-regulated, respectively, by one or more of the three chemicals. Of the transcripts shared by responses to all three chemicals, 85% of up-regulated transcripts and 75% of down-regulated transcripts had the same magnitude of response. A total of 290 and 110 unique proteins were up- and down-regulated, respectively, by one or more of the three chemicals. Of the proteins shared by responses to all three chemicals, 70% of up-regulated proteins and 48% of down-regulated proteins had the same magnitude of response. Among treatments there was 68% similarity between the global transcriptome and global proteome. Pathway analysis revealed that perturbed physiological processes were indistinguishable between equipotent concentrations of the three chemicals. The results of this study contribute toward more completely describing adverse outcome pathways associated with activation of the AHR.
authors.affiliation: Toxicology Graduate Program, University of Saskatchewan , 44 Campus Drive, Saskatoon, SK S7N 5B3, Canada.|Toxicology Centre, University of Saskatchewan , 44 Campus Drive, Saskatoon, SK S7N 5B3, Canada.
authors.forename: Jon A
authors.initials: JA
authors.lastname: Doering
authors.affiliation: School of Environment and Sustainability, University of Saskatchewan , Saskatoon, SK S7N 5C8, Canada.
authors.forename: Song
authors.initials: S
authors.lastname: Tang
authors.affiliation: Toxicology Centre, University of Saskatchewan , 44 Campus Drive, Saskatoon, SK S7N 5B3, Canada.
authors.forename: Hui
authors.initials: H
authors.lastname: Peng
authors.affiliation: Toxicology Centre, University of Saskatchewan , 44 Campus Drive, Saskatoon, SK S7N 5B3, Canada.
authors.forename: Bryanna K
authors.initials: BK
authors.lastname: Eisner
authors.affiliation: Toxicology Centre, University of Saskatchewan , 44 Campus Drive, Saskatoon, SK S7N 5B3, Canada.
authors.forename: Jianxian
authors.initials: J
authors.lastname: Sun
authors.affiliation: Toxicology Centre, University of Saskatchewan , 44 Campus Drive, Saskatoon, SK S7N 5B3, Canada.|Department of Veterinary Biomedical Sciences, University of Saskatchewan , Saskatoon, SK S7N 5B4, Canada.
authors.forename: John P
authors.initials: JP
authors.lastname: Giesy
authors.affiliation: Toxicology Centre, University of Saskatchewan , 44 Campus Drive, Saskatoon, SK S7N 5B3, Canada.
authors.forename: Steve
authors.initials: S
authors.lastname: Wiseman
authors.affiliation: Toxicology Centre, University of Saskatchewan , 44 Campus Drive, Saskatoon, SK S7N 5B3, Canada.|School of Environment and Sustainability, University of Saskatchewan , Saskatoon, SK S7N 5C8, Canada.
authors.forename: Markus
authors.initials: M
authors.lastname: Hecker
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene; D004147:Dioxins; D005399:Fishes; D000072317:Polychlorinated Dibenzodioxins; D040901:Proteomics
chemical_list: D004147:Dioxins; D000072317:Polychlorinated Dibenzodioxins; D001564:Benzo(a)pyrene