title: Establishment of a colorectal cancer nude mouse visualization model of HIF-1α overexpression.
abstract: The aim of the present study was to establish a model of tumor cell growth and visualize HIF-1α overexpression in a nude mouse xenograft model of colorectal cancer (CRC). In the study, HIF-1α lentiviral vector and helper plasmid were co-transfected into 293T packaging cells using a liposome method, and the virus was collected following transfection and used to infect CRC SW480, SW620, LoVo and HCT116 cells. Puromycin was used for the selection and large-scale amplification of the stable HIF-1α expression of green fluorescent protein (GFP)-positive cells. HIF-1α-expressing cells were injected intraperitoneally into a nude mouse xenograft model, and resulting tumor nodules was separated and confirmed using an inverted fluorescence microscope. The results demonstrated that HIF-1α was not expressed in CRC cells in normoxic conditions. When treated with CoCl2, the expression of HIF-1α could be induced in all the cancer cell lines, except SW480. HIF-1α was highly expressed following infection with lentiviral particles. Stable expression of HIF-1α promoted migration in the SW480 cells. Following intraperitoneal injection of nude mice with SW480-HIF-1α, a significant number of tumor nodules formed in the intestinal wall compared with the controls (P<0.05). The successful construction of the dual expression HIF-1α and GFP visualization xenograft model provides a good foundation for the screening of HIF-1α-related functions and for investigating the therapeutic potential of drugs that target HIF-1α.
authors.affiliation: Department of Thyroid and Breast Surgery, The Shanghai Tenth People's Hospital, Tong Ji University, Shanghai 200072, P.R. China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Criminal Science and Technology, Zhejiang Police College, Hangzhou, Zhejiang 310053, P.R. China.
authors.forename: Jiye
authors.initials: J
authors.lastname: Wang
authors.affiliation: Hepatobiliary Surgery Centre, The Ningbo No. 2 Hospital, Ningbo, Zhejiang 315010, P.R. China.
authors.forename: Tianbao
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Division of Drugs and Pharamcology, Ningbo Institute of Medical Sciences, Ningbo, Zhejiang 315020, P.R. China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Ye
keywords: HIF-1α; colorectal cancer; green fluorescent protein; lenti virus; stable transfection; xenograft model