title: Metabolic Engineering of Microalgal Based Biofuel Production: Prospects and Challenges.
abstract: The current scenario in renewable energy is focused on development of alternate and sustainable energy sources, amongst which microalgae stands as one of the promising feedstock for biofuel production. It is well known that microalgae generate much larger amounts of biofuels in a shorter time than other sources based on plant seeds. However, the greatest challenge in a transition to algae-based biofuel production is the various other complications involved in microalgal cultivation, its harvesting, concentration, drying and lipid extraction. Several green microalgae accumulate lipids, especially triacylglycerols (TAGs), which are main precursors in the production of lipid. The various aspects on metabolic pathway analysis of an oleaginous microalgae i.e., Chlamydomonas reinhardtii have elucidated some novel metabolically important genes and this enhances the lipid production in this microalgae. Adding to it, various other aspects in metabolic engineering using OptFlux and effectual bioprocess design also gives an interactive snapshot of enhancing lipid production which ultimately improvises the oil yield. This article reviews the current status of microalgal based technologies for biofuel production, bioreactor process design, flux analysis and it also provides various strategies to increase lipids accumulation via metabolic engineering.
authors.affiliation: Department of Environmental Science and Engineering, Indian School of Mines Dhanbad, India.
authors.forename: Chiranjib
authors.initials: C
authors.lastname: Banerjee
authors.affiliation: Department of Biotechnology, University Institute of Engineering and Technology, Maharshi Dayanand University Rohtak, India.
authors.forename: Kashyap K
authors.initials: KK
authors.lastname: Dubey
authors.affiliation: Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University Rohtak, India.
authors.forename: Pratyoosh
authors.initials: P
authors.lastname: Shukla
keywords: Chlamydomonas reinhardtii; biofuel; bioreactor; metabolic engineering; microalgae