title: [Protective Effect of Angiotensin Converting Enzyme 2 (ACE2) Against Chronic Intermittent Hypoxia-induced Pulmonary Oxidative Stress Injury in Rats].
abstract: OBJECTIVE
  To determine changes of angiotensin converting enzyme 2 (ACE2) in the development of chronic intermittent hypoxia (CIH)-induced pulmonary injury in rats and its mechanism.
  
  
  METHODS
  96 adult male Wistar rats were randomly allocated into four groups: control (UC) group, chronic intermittent hypoxia (CIH) group, normal saline treated CIH (NS) group, and edaravone treated CIH (NE) group. Each group contained four subgroups of rats (n = 6) subjecting to 1 week, 2 weeks, 3 weeks, and 4 weeks experiment, respectively. No intervention was given to rats in the UC group. Rats in the experimental groups were exposed to alternating cycles of nitrogen and compressed air. Rats in the NS and NE groups received daily injection of 0.9% normal saline (3 mg/ kg) and edaravone (3 mg/kg), respectively. Pulmonary sections were taken and stained with hematoxylin-eosin (HE). The levels of malondialdehyde (MDA), ACE2, ACE2 mRNA, and angiotensin II (Ang II) mRNA in the rat homogenate pulmonary samples were measured.
  
  
  RESULTS
  Rats in the CIH and NS groups showed high levels of interstitial edema, alveolar atelectasis, and inflammatory cell infiltration in alveolar epithelial cells. The pulmonary injury got worse over time. Rats in the NE group showed later occurrence-and milder pulmonary injury compared with those in the NS group. Rats in the CIH and NS groups had higher levels of MDA and Ang II mRNA (which increased over time) than those in the UC group (P < 0.05). The expression of ACE2 and the level of ACE2 mRNA increased in rats in the CIH group (P < 0.05), and peaked at 2 weeks (P < 0.05). Rats in the NE group had moderately increased levels of MDA and Ang II mRNA compared with those in the NS group (P < 0.05); moderately increased levels of expression of ACE2 and ACE2 mRNA compared with those in the UC and SC groups (P < 0.05). The pulmonary level of Ang II mRNA was positively correlated with MDA (r = 0.782, P < 0.01) in rats in the CIH group.
  
  
  CONCLUSIONS
  CIH can activate oxidation stress and Ang II, which maybe an important mechanism of CIH-induced pulmonary injury.
authors.forename: Yu-le
authors.initials: YL
authors.lastname: Kou
authors.forename: Pan-pan
authors.initials: PP
authors.lastname: Zhang
authors.forename: Hong-yang
authors.initials: HY
authors.lastname: Wang
authors.forename: Jia-bin
authors.initials: JB
authors.lastname: Zhang
authors.forename: Xi-shu
authors.initials: XS
authors.lastname: Tan
authors.forename: Chao
authors.initials: C
authors.lastname: Huang
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000804:Angiotensin II / Q000378:metabolism; D000085962:Angiotensin-Converting Enzyme 2; D000818:Animals; D000860:Hypoxia / Q000473:pathology*; D008168:Lung / Q000378:metabolism; D055370:Lung Injury / Q000378:metabolism* / Q000473:pathology; D008297:Male; D018384:Oxidative Stress; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D012333:RNA, Messenger; D051381:Rats; D017208:Rats, Wistar
chemical_list: D012333:RNA, Messenger; D000804:Angiotensin II; D007703:Peptidyl-Dipeptidase A; C000705329:Ace2 protein, rat; D000085962:Angiotensin-Converting Enzyme 2