title: Recovery of Syrian hamster hippocampal signaling following its depression during oxygen-glucose deprivation is enhanced by cold temperatures and by hibernation.
abstract: Signal transmission over a hippocampal network of CA3 and CA1 neurons in Syrian hamsters (Mesocricetus auratus), facultative hibernators, has not been fully characterized in response to oxygen-glucose deprivation (OGD). We hypothesized that during OGD, hippocampal signal transmission fails first at the synapse between CA3 and CA1 pyramidal neurons and that recovery of signal processing following OGD is more robust in hippocampal slices at cold temperature, from hamsters vs. rats, and from hibernating vs. non-hibernating hamsters. To test these hypotheses, we recorded fEPSPs and population spikes of CA1 neurons at 25°C, 30°C, and 35°C in 400μm slices over a 15min control period with the slice in oxygenated aCSF containing glucose (control solution), a 10min treatment period (OGD insult) where oxygen was replaced by nitrogen in aCSF lacking glucose, and a 30min recovery period with the slice in the control solution. The initial site of transmission failure during OGD occurred at the CA3-CA1 synapse, and recovery of signal transmission was at least, if not more (depending on temperature), complete in slices from hibernating vs. non-hibernating hamsters, and from non-hibernating hamsters vs. rats. Thus, hamster neuroprotective mechanisms supporting functional recovery were enhanced by cold temperatures and by hibernation.
authors.affiliation: Dept. of Neurobiology, Physiology & Behavior, College of Biological Sciences, University of California, Davis, One Shields Ave., Davis, CA 95616, United States.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Mikhailova
authors.affiliation: Dept. of Neurobiology, Physiology & Behavior, College of Biological Sciences, University of California, Davis, One Shields Ave., Davis, CA 95616, United States.
authors.forename: Jacob
authors.initials: J
authors.lastname: Mack
authors.affiliation: Dept. of Neurobiology, Physiology & Behavior, College of Biological Sciences, University of California, Davis, One Shields Ave., Davis, CA 95616, United States.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Vitagliano
authors.affiliation: Dept. of Neurobiology, Physiology & Behavior, College of Biological Sciences, University of California, Davis, One Shields Ave., Davis, CA 95616, United States.
authors.forename: Jock S
authors.initials: JS
authors.lastname: Hamilton
authors.affiliation: Dept. of Neurobiology, Physiology & Behavior, College of Biological Sciences, University of California, Davis, One Shields Ave., Davis, CA 95616, United States.
authors.forename: John M
authors.initials: JM
authors.lastname: Horowitz
authors.affiliation: Dept. of Neurobiology, Physiology & Behavior, College of Biological Sciences, University of California, Davis, One Shields Ave., Davis, CA 95616, United States. Electronic address: bahorwitz@ucdavis.edu.
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Horwitz
mesh_terms: D000818:Animals; D056547:CA1 Region, Hippocampal / Q000378:metabolism; D056654:CA3 Region, Hippocampal / Q000378:metabolism; D003080:Cold Temperature; D006224:Cricetinae; D003863:Depression / Q000503:physiopathology*; D005947:Glucose / Q000172:deficiency*; D006605:Hibernation; D006624:Hippocampus / Q000503:physiopathology*; D008647:Mesocricetus; D010100:Oxygen / Q000378:metabolism*; D015398:Signal Transduction; D013569:Synapses / Q000378:metabolism
chemical_list: D005947:Glucose; D010100:Oxygen
keywords: CA1 pyramidal cells; CA1-CA3 synapse; Hibernati; Oxygen deprivation; Population spike; fEPSP