title: Identification and expression of a new Ly6 gene cluster in zebrafish Danio rerio, with implications of being involved in embryonic immunity.
abstract: Lymphocyte antigen-6 (Ly6) superfamily is a large family of proteins and characterized by precisely spaced cysteine motifs, termed the three-finger fold. To date, a large number of members of the Ly6/uPAR family were identified among many species. In this study, we first report the identification and characterization of the secreted Ly2.1-3 proteins on the chromosome 2 in zebrafish and determine the expression pattern. Ly2.1-3 all possess a conserved LU domain and adopt similar three-finger structure with human CD59, SLURP1 and other Ly6 family members. Ly2.1-3 cluster on chromosome 2 and share high homology, possibly originated from chromosomal gene duplication. Ly2.1-3 exhibit distinct expression pattern in the endoderm, they were found abundantly and specifically in the digestive tract, liver and pancreas respectively. The differential expression pattern may suggest Ly2.1-3 acquire new function during gene duplication. The expression level of Ly2.1-3 were up-regulating challenged with LPS indicated that they have a role in innate immune responses of the digestive system during endotoxin challenge in early stage.
authors.affiliation: Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China; Laboratory for Evolution & Development, Department of Marine Biology, Ocean University of China, Qingdao 266003, China.
authors.forename: Man
authors.initials: M
authors.lastname: Wang
authors.affiliation: Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China; Laboratory for Evolution & Development, Department of Marine Biology, Ocean University of China, Qingdao 266003, China.
authors.forename: Lingyi
authors.initials: L
authors.lastname: Li
authors.affiliation: Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China; Laboratory for Evolution & Development, Department of Marine Biology, Ocean University of China, Qingdao 266003, China.
authors.forename: Quanyang
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China; Laboratory for Evolution & Development, Department of Marine Biology, Ocean University of China, Qingdao 266003, China.
authors.forename: Shicui
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China; Laboratory for Evolution & Development, Department of Marine Biology, Ocean University of China, Qingdao 266003, China. Electronic address: dongruij@yahoo.com.
authors.forename: Dongrui
authors.initials: D
authors.lastname: Ji
authors.affiliation: Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China; Laboratory for Evolution & Development, Department of Marine Biology, Ocean University of China, Qingdao 266003, China. Electronic address: hongyanli@ouc.edu.cn.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000950:Antigens, Ly / Q000235:genetics* / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000276:immunology; D018507:Gene Expression Regulation, Developmental; D007113:Immunity, Innate; D005810:Multigene Family; D011487:Protein Conformation; D016415:Sequence Alignment / Q000662:veterinary; D015027:Zebrafish / Q000196:embryology / Q000235:genetics* / Q000276:immunology; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D000950:Antigens, Ly; D029961:Zebrafish Proteins
keywords: Digestive system; Immunity; LPS; Ly6 superfamily; Zebrafish