title: Cytogenetic Biomonitoring in Buccal Mucosa Cells from Women Submitted to Chemotherapy After Mastectomy for Breast Cancer.
abstract: In addition to surgery, one of the most widely applied treatments for breast cancer is chemotherapy. Chemotherapy is currently considered efficient in curing this disease; however, the therapy may induce damage to the patient's genetic material. Thus, the aim of this study was to evaluate putative cytotoxic and mutagenic effects induced by chemotherapy in women diagnosed with breast cancer. For this purpose, a cross-sectional study was carried out in 42 women, aged 18 to 70 years, allocated according to the diagnosis and stage of breast cancer treatment: control group (healthy) (n=15), chemotherapy group (n=11) and post-chemotherapy group (n=16). Cytotoxicity and mutagenicity were analyzed by the micronucleus test in buccal mucosa cells. A higher frequency (p<0.05) of micronucleated cells was detected in the chemotherapy and post-chemotherapy groups when compared to the control. A higher frequency (p<0.05) of karyorrhexis and pyknosis in the chemotherapy group was also noted. Taken together, our results indicate that chemotherapy induces mutagenicity and cytotoxicity in buccal mucosa cells of women diagnosed with breast cancer, being persistent after finishing their treatment.
authors.affiliation: Department of Biosciences, Federal University of Sao Paulo (UNIFESP), Sao Paulo, SP, Brazil.
authors.forename: Ana Carolina Flygare
authors.initials: AC
authors.lastname: Souza
authors.affiliation: Department of Biosciences, Federal University of Sao Paulo (UNIFESP), Sao Paulo, SP, Brazil.
authors.forename: Victor Hugo Pereira
authors.initials: VH
authors.lastname: DA Silva
authors.affiliation: Department of Biosciences, Federal University of Sao Paulo (UNIFESP), Sao Paulo, SP, Brazil.
authors.forename: Camila
authors.initials: C
authors.lastname: Seixas
authors.affiliation: Department of Management and Health Care, Federal University of Sao Paulo (UNIFESP), Sao Paulo, SP, Brazil.
authors.forename: Tania Terezinha
authors.initials: TT
authors.lastname: DE Oliveira Scudeller
authors.affiliation: Department of Management and Health Care, Federal University of Sao Paulo (UNIFESP), Sao Paulo, SP, Brazil.
authors.forename: Maria Teresa Pace
authors.initials: MT
authors.lastname: DO Amaral
authors.affiliation: Department of Biosciences, Federal University of Sao Paulo (UNIFESP), Sao Paulo, SP, Brazil daribeiro@unifesp.br daribeiro@pesquisador.cnpq.br.
authors.forename: Daniel Araki
authors.initials: DA
authors.lastname: Ribeiro
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000970:Antineoplastic Agents / Q000009:adverse effects* / Q000627:therapeutic use; D001943:Breast Neoplasms / Q000188:drug therapy / Q000601:surgery; D018270:Carcinoma, Ductal, Breast / Q000188:drug therapy* / Q000601:surgery; D020732:Cytogenetic Analysis; D005260:Female; D006801:Humans; D008408:Mastectomy; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced*; D015162:Micronucleus Tests; D008875:Middle Aged; D009061:Mouth Mucosa / Q000166:cytology / Q000187:drug effects*; D055815:Young Adult
chemical_list: D000970:Antineoplastic Agents
keywords: Oral mucosa cells; breast cancer; chemotherapy; micronucleus test