title: Novel mutants of the aubergine gene.
abstract: Aubergine is an RNA-binding protein of the Piwi clade, functioning in germline in the piRNA pathway that silences transposons and repetitive sequences. Several mutations of this gene exist, but they mostly result in truncated proteins or correspond to mutations that also affect neighboring genes. We have generated complete aubergine knock-out mutants that do not disrupt the neighboring genes. These novel mutants are characterized by PCR and sequencing. Their nature is confirmed by female sterility and by the presence of crystals in testes, common to the aubergine loss of function mutations. These mutants provide novel and more appropriate tools for the study of the piRNA pathway that controls genome stability.
authors.affiliation: a IGBMC, CNRS UMR 7104 - Inserm U 964 . Illkirch Cedex / FRANCE.|b Current address: Kadir Has University , Department of Bioinformatics and Genetics , Fatih , İstanbul / TURKEY.
authors.forename: H Bahar
authors.initials: HB
authors.lastname: Sahin
authors.affiliation: a IGBMC, CNRS UMR 7104 - Inserm U 964 . Illkirch Cedex / FRANCE.|c Current address: Department of Molecular Biology and Genetics, Erzurum Technical University , Erzurum / TURKEY.
authors.forename: Omer Faruk
authors.initials: OF
authors.lastname: Karatas
authors.affiliation: d DiSTeBA - Department of Biological and Environmental Science and Technology - University of Salento - Lecce , Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Specchia
authors.affiliation: d DiSTeBA - Department of Biological and Environmental Science and Technology - University of Salento - Lecce , Italy.
authors.forename: Silvia Di
authors.initials: SD
authors.lastname: Tommaso
authors.affiliation: a IGBMC, CNRS UMR 7104 - Inserm U 964 . Illkirch Cedex / FRANCE.
authors.forename: Céline
authors.initials: C
authors.lastname: Diebold
authors.affiliation: d DiSTeBA - Department of Biological and Environmental Science and Technology - University of Salento - Lecce , Italy.
authors.forename: Maria Pia
authors.initials: MP
authors.lastname: Bozzetti
authors.affiliation: a IGBMC, CNRS UMR 7104 - Inserm U 964 . Illkirch Cedex / FRANCE.
authors.forename: Angela
authors.initials: A
authors.lastname: Giangrande
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D055786:Gene Knockout Techniques / Q000379:methods*; D009154:Mutation; D010448:Peptide Initiation Factors / Q000235:genetics*
chemical_list: D029721:Drosophila Proteins; D010448:Peptide Initiation Factors; C436124:aub protein, Drosophila
keywords: Aubergine; argonaute; crystal-stellate; knockout mutant; mutagenesis; piRNA; sterility