title: Construction of antibacterial poly(ethylene terephthalate) films via layer by layer assembly of chitosan and hyaluronic acid.
abstract: Polyelectrolytic multilayers (PEMs) with enhanced antibacterial properties were built up onto commercial poly(ethylene terephthalate) (PET) films based on the layer by layer assembling of bacterial contact killing chitosan and bacterial repelling highly hydrated hyaluronic acid. The optimization of the aminolysis modification reaction of PET was carried out by the study of the mechanical properties and the surface characterization of the modified polymers. The layer by layer assembly was successfully monitored by TEM microscopy, surface zeta-potential, contact angle measurements and, after labeling with fluorescein isothiocyanate (FTIC) by absorption spectroscopy and confocal fluorescent microscopy. Beside, the stability of the PEMs was studied at physiological conditions in absence and in the presence of lysozyme and hyaluronidase enzymes. Antibacterial properties of the obtained PEMs against Escherichia coli were compared with original commercial PET.
authors.affiliation: Departamento de Química Física (Laboratorio de Química Macromolecular), Universidad del País Vasco (UPV/EHU), B° Sarriena s/n, 48940 Leioa, Vizcaya, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Del Hoyo-Gallego
authors.affiliation: Departamento de Química Física (Laboratorio de Química Macromolecular), Universidad del País Vasco (UPV/EHU), B° Sarriena s/n, 48940 Leioa, Vizcaya, Spain; BCMaterials, Bizkaia Science and Technology Park, Building 500-1st Floor, 48160 Derio, Spain. Electronic address: leyre.perez@ehu.es.
authors.forename: Leyre
authors.initials: L
authors.lastname: Pérez-Álvarez
authors.affiliation: Departamento de Química Física (Laboratorio de Química Macromolecular), Universidad del País Vasco (UPV/EHU), B° Sarriena s/n, 48940 Leioa, Vizcaya, Spain.
authors.forename: Flor
authors.initials: F
authors.lastname: Gómez-Galván
authors.affiliation: Departamento de Química Física (Laboratorio de Química Macromolecular), Universidad del País Vasco (UPV/EHU), B° Sarriena s/n, 48940 Leioa, Vizcaya, Spain.
authors.forename: Erlantz
authors.initials: E
authors.lastname: Lizundia
authors.affiliation: Centre of Polymer Systems, Tomas Bata University in Zlín, trida Tomase Bati 5678, Zlin 76001, Czech Republic.
authors.forename: Ivo
authors.initials: I
authors.lastname: Kuritka
authors.affiliation: Centre of Polymer Systems, Tomas Bata University in Zlín, trida Tomase Bati 5678, Zlin 76001, Czech Republic.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Sedlarik
authors.affiliation: Departamento de Química Física (Laboratorio de Química Macromolecular), Universidad del País Vasco (UPV/EHU), B° Sarriena s/n, 48940 Leioa, Vizcaya, Spain.
authors.forename: Jose Manuel
authors.initials: JM
authors.lastname: Laza
authors.affiliation: Departamento de Química Física (Laboratorio de Química Macromolecular), Universidad del País Vasco (UPV/EHU), B° Sarriena s/n, 48940 Leioa, Vizcaya, Spain.
authors.forename: Jose Luis
authors.initials: JL
authors.lastname: Vila-Vilela
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D006820:Hyaluronic Acid / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000494:pharmacology; D006821:Hyaluronoglucosaminidase / Q000737:chemistry; D009113:Muramidase / Q000737:chemistry; D011093:Polyethylene Terephthalates / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D011093:Polyethylene Terephthalates; D006820:Hyaluronic Acid; D048271:Chitosan; D009113:Muramidase; D006821:Hyaluronoglucosaminidase; D016650:Fluorescein-5-isothiocyanate
keywords: Chitosan; Hyaluronic acid; Poly(ethylene terephthalate) aminolysis; Polyelectrolyte multilayers