title: Selection of Vaccine Candidates for Fish Pasteurellosis Using Reverse Vaccinology and an In Vitro Screening Approach.
abstract: The advent of new technologies in recent years has revolutionized the methods by which pathogens are studied and at the same time it has provided new tools to design vaccines against infections for which vaccine development has so far been unsuccessful. The availability of genomic data provides the basis for the reverse vaccinology approach, a biotechnological strategy that uses bioinformatics analysis of microbial genome data for the in silico selection of potential vaccine candidates for the development of protein-based vaccines. The antigens selected by reverse vaccinology can be produced as recombinant proteins and subjected to further in vitro screening assays before in vivo experiments to assess immunogenicity and protection. The reverse vaccinology approach has been applied to several pathogens affecting human health, but also to marine bacteria, including Photobacterium damselae subsp. piscicida causing significant harm in marine aquaculture.
authors.affiliation: Department of Biomolecular Science-Section of Biotechnology, University of Urbino "Carlo Bo", Via Arco d'Augusto 2, Fano, 61032, Italy. francesca.andreoni@uniurb.it.
authors.forename: Francesca
authors.initials: F
authors.lastname: Andreoni
authors.affiliation: Department of Biomolecular Science-Section of Biotechnology, University of Urbino "Carlo Bo", Via Arco d'Augusto 2, Fano, 61032, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Amagliani
authors.affiliation: Department of Biomolecular Science-Section of Biotechnology, University of Urbino "Carlo Bo", Via Arco d'Augusto 2, Fano, 61032, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Magnani
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D002448:Cell Adhesion; D002460:Cell Line; D019295:Computational Biology / Q000379:methods*; D005399:Fishes / Q000382:microbiology*; D010325:Pasteurella / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D001428:Bacterial Vaccines
keywords: Adherence inhibition assay; Photobacteriosis; Photobacterium damselae subsp. piscicida; Reverse vaccinology; Vaccine candidate selection