title: DNA damage in hemodialysis patients with chronic kidney disease; a test of the role of diabetes mellitus; a comet assay investigation.
abstract: The incidence of chronic kidney disease (CKD) is increasing rapidly. Diabetes mellitus (DM) is the most important cause of CKD. We studied the possible role of DM in CKD patients with respect to DNA damage, as assessed by the comet assay in 60 CKD patients (with or without DM) undergoing hemodialysis and in 26 controls. Effects of other factors, such as age, sex, hypertension, duration of hemodialysis, body mass index (BMI), and levels of hemoglobin (HB), intact parathormone (iPTH), and ferritin (FER), were also examined. Primary DNA damage measured by the comet assay was significantly higher in CKD patients than in controls. Among CKD patients, the following correlations were observed. (1) There was no difference in comet tail length or tail intensity between diabetic and non-diabetic individuals. (2) Age, sex, hemoglobin, hypertension, duration of hemodialysis, and ferritin levels affected neither tail length nor intensity. (3) BMI values above 25kg/m(2) and iPTH levels above 300pg/ml were associated with significantly greater comet tail length. Our results indicate that primary DNA damage is increased in CKD patients undergoing hemodialysis, compared to controls; however, DM had no additional effect.
authors.affiliation: Department of Biology, Polatlı Art and Science Faculty, Gazi University, Ankara, Turkey.
authors.forename: Sevcan
authors.initials: S
authors.lastname: Mamur
authors.affiliation: Department of Biology, Science Faculty, Gazi University, Ankara, Turkey.
authors.forename: Fatma
authors.initials: F
authors.lastname: Unal
authors.affiliation: Department of Nephrology, Medical Faculty, Gazi University, Ankara, Turkey.
authors.forename: Kadriye
authors.initials: K
authors.lastname: Altok
authors.affiliation: Department of Nephrology, Medical Faculty, Gazi University, Ankara, Turkey.
authors.forename: Serpil Muge
authors.initials: SM
authors.lastname: Deger
authors.affiliation: Department of Biology, Science Faculty, Gazi University, Ankara, Turkey. Electronic address: deniz@gazi.edu.tr.
authors.forename: Deniz
authors.initials: D
authors.lastname: Yuzbasioglu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D020552:Comet Assay / Q000379:methods; D015897:Comorbidity; D004249:DNA Damage; D003920:Diabetes Mellitus / Q000453:epidemiology; D005260:Female; D006801:Humans; D015994:Incidence; D016014:Linear Models; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D006435:Renal Dialysis; D051436:Renal Insufficiency, Chronic / Q000453:epidemiology / Q000235:genetics* / Q000628:therapy*; D012307:Risk Factors; D014421:Turkey / Q000453:epidemiology; D055815:Young Adult
keywords: Chronic kidney disease (CKD); Comet assay; Diabetes mellitus (DM); Hemodialysis (HD); Human lymphocytes