title: Upregulation of microRNA‑337 promotes the proliferation of endometrial carcinoma cells via targeting PTEN.
abstract: Endometrial carcinoma (EC) is a common malignancy in females. MicroRNAs (miRs) are a class of non‑coding RNA that regulate a wide variety of cellular processes, and are important in the development of multiple types of malignancy. In the present study, cancerous and adjacent non‑cancerous normal tissue samples were collected from 24 patients diagnosed with EC. Reverse transcription quantitative polymerase chain reaction was performed on the tissue samples to determine the expression levels of six candidate miRs. These miRs have been previously reported to be differentially expressed in EC; however, the present study observed that only miR‑337 was differentially expressed. In addition, the current study identified phosphatase and tensin homolog (PTEN) as a target of miR‑337 using computational analysis and a luciferase assay. EC cells transfected with miR‑337 mimics and anti‑PTEN small interfering RNA demonstrated significantly decreased expression of PTEN, markedly increased proliferation and inhibition of cell apoptosis. The results indicate that miR‑337 is oncogenic in EC cells, as it suppresses PTEN expression. This may facilitate the development of miR‑based prevention or treatment strategies for EC.
authors.affiliation: Department of Gynaecology and Obstetrics, First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan 471000, P.R. China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Department of Gynaecology and Obstetrics, First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan 471000, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: He
authors.affiliation: Department of Gynaecology and Obstetrics, First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan 471000, P.R. China.
authors.forename: Lidan
authors.initials: L
authors.lastname: Liang
authors.affiliation: Department of Gynaecology and Obstetrics, First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan 471000, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Pathogenic Biology, Henan University of Science and Technology, Luoyang, Henan 471000, P.R. China.
authors.forename: Hongying
authors.initials: H
authors.lastname: Yuan
mesh_terms: D020413:3' Untranslated Regions; D000368:Aged; D001665:Binding Sites; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D051059:PTEN Phosphohydrolase / Q000235:genetics*; D034622:RNA Interference
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; C544075:Mirn337 microRNA, human; D051059:PTEN Phosphohydrolase