title: A "signal on" protection-displacement-hybridization-based electrochemical hepatitis B virus gene sequence sensor with high sensitivity and peculiar adjustable specificity.
abstract: One "signal on" electrochemical sensing strategy was constructed for the detection of a specific hepatitis B virus (HBV) gene sequence based on the protection-displacement-hybridization-based (PDHB) signaling mechanism. This sensing system is composed of three probes, one capturing probe (CP) and one assistant probe (AP) which are co-immobilized on the Au electrode surface, and one 3-methylene blue (MB) modified signaling probe (SP) free in the detection solution. One duplex are formed between AP and SP with the target, a specific HBV gene sequence, hybridizing with CP. This structure can drive the MB labels close to the electrode surface, thereby producing a large detection current. Two electrochemical testing techniques, alternating current voltammetry (ACV) and cyclic voltammetry (CV), were used for characterizing the sensor. Under the optimized conditions, the proposed sensor exhibits a high sensitivity with the detection limit of ∼5fM for the target. When used for the discrimination of point mutation, the sensor also features an outstanding ability and its peculiar high adjustability.
authors.affiliation: College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150040, China.
authors.forename: Fengqin
authors.initials: F
authors.lastname: Li
authors.affiliation: College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150040, China.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Xu
authors.affiliation: College of Chemical and Environmental Engineering, Guangdong University of Petrochemical Technology, Maoming 525000, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Yu
authors.affiliation: College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150040, China. Electronic address: yzgyhdzm@126.com.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Department of Electronic Science and Technology, Harbin University of Science and Technology, Harbin 150080, China.
authors.forename: Xunjun
authors.initials: X
authors.lastname: He
authors.affiliation: College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150040, China.
authors.forename: Hongrui
authors.initials: H
authors.lastname: Ji
authors.affiliation: College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150040, China.
authors.forename: Jinghao
authors.initials: J
authors.lastname: Dong
authors.affiliation: College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150040, China.
authors.forename: Yongbin
authors.initials: Y
authors.lastname: Song
authors.affiliation: College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150040, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yan
authors.affiliation: College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150040, China. Electronic address: Zhangguiling1969@126.com.
authors.forename: Guiling
authors.initials: G
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006509:Hepatitis B / Q000821:virology*; D006515:Hepatitis B virus / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D057230:Limit of Detection; D008751:Methylene Blue / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D017354:Point Mutation
chemical_list: D006046:Gold; D008751:Methylene Blue
keywords: Electrochemical sensor; Hepatitis B virus gene sequence; Methylene blue; Point mutation discrimination