title: CAR-T Cell Therapies From the Transfusion Medicine Perspective.
abstract: The use of chimeric antigen receptor (CAR)-T cell therapy for the treatment of hematologic malignancies has generated significant excitement over the last several years. From a transfusion medicine perspective, the implementation of CAR-T therapy as a potential mainstay treatment for not only hematologic but also solid-organ malignancies represents a significant opportunity for growth and expansion. In this review, we will describe the rationale for the development of genetically redirected T cells as a cancer therapeutic, the different elements that are required to engineer these cells, as well as an overview of the process by which patient cells are harvested and processed to create and subsequently validate CAR-T cells. Finally, we will briefly describe some of the toxicities and clinical efficacy of CAR-T cells in the setting of patients with advanced malignancy.
authors.affiliation: Division of Transfusion Medicine & Therapeutic Pathology, Department of Pathology & Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Fesnak
authors.affiliation: Massachusetts General Hospital Cancer Center, Harvard Medical School, Boston, MA.
authors.forename: ChieYu
authors.initials: C
authors.lastname: Lin
authors.affiliation: Division of Transfusion Medicine & Therapeutic Pathology, Department of Pathology & Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA.
authors.forename: Don L
authors.initials: DL
authors.lastname: Siegel
authors.affiliation: Massachusetts General Hospital Cancer Center, Harvard Medical School, Boston, MA. Electronic address: mvmaus@mgh.harvard.edu.
authors.forename: Marcela V
authors.initials: MV
authors.lastname: Maus
mesh_terms: D019337:Hematologic Neoplasms / Q000628:therapy*; D006801:Humans; D016219:Immunotherapy, Adoptive; D011948:Receptors, Antigen, T-Cell; D013601:T-Lymphocytes / Q000637:transplantation*; D064826:Transfusion Medicine
chemical_list: D011948:Receptors, Antigen, T-Cell
keywords: Adoptive immunotherapy; Cell processing; Gene transfer; Leukapheresis; T cells