title: Identification and expression analysis of duck interleukin-17D in Riemerella anatipestifer infection.
abstract: Interleukin (IL)-17D is a proinflammatory cytokine with currently largely unknown biological functions. Here we provide the description of the sequence, bioactivity, and mRNA expression profile of duck IL-17D homologue. A full-length duck IL-17D (duIL-17D) cDNA with a 624-bp coding region was identified from the large intestine. duIL-17D shares approximately 94.7% identity with its chicken counterpart, which is also identified in this work. duIL-17D exhibits 62.6-68.4% and 52.1-53.1% identity with mammalian and piscine homologues. Recombinant duIL-17D promoted the expression of proinflammatory cytokines such as IL-6, IL-8, and IL-1β in duck embryo fibroblast cells. Very low levels of duIL-17D transcript were observed in healthy lymphoid tissues, including bursa, thymus, and spleen, while duIL-17D expression was relatively high in the heart. The duIL-17D expression profiles were examined in mitogen-stimulated splenic lymphocytes, as well as tissues affected by Riemerella anatipestifer infection. The levels of duIL-17D were mostly upregulated in mitogen-activated splenic lymphocytes but downregulated in the liver and spleen of R. anatipestifer-infected ducks. These results provide new insights into the roles of IL-17D in host protective immune responses to Riemerella infection, which can therefore lead to further studies of its biological functions in different disease models of ducks and other avian species.
authors.affiliation: College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University, Jinju, 52828, Republic of Korea.
authors.forename: Joyce Anne R
authors.initials: JA
authors.lastname: Diaz
authors.affiliation: College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University, Jinju, 52828, Republic of Korea.
authors.forename: Woo H
authors.initials: WH
authors.lastname: Kim
authors.affiliation: College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University, Jinju, 52828, Republic of Korea.
authors.forename: Cherry P
authors.initials: CP
authors.lastname: Fernandez
authors.affiliation: Environmental Health Research Division, National Institute of Environmental Research, Incheon, 22689, Republic of Korea.
authors.forename: Jipseol
authors.initials: J
authors.lastname: Jeong
authors.affiliation: College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University, Jinju, 52828, Republic of Korea.
authors.forename: Fahmida
authors.initials: F
authors.lastname: Afrin
authors.affiliation: Animal Biosciences and Biotechnology Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD, 20705, USA.
authors.forename: Hyun S
authors.initials: HS
authors.lastname: Lillehoj
authors.affiliation: College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University, Jinju, 52828, Republic of Korea.
authors.forename: Suk
authors.initials: S
authors.lastname: Kim
authors.affiliation: Avian Immunobiology Laboratory, Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA, 24061, USA.
authors.forename: Sungwon
authors.initials: S
authors.lastname: Kim
authors.affiliation: Avian Immunobiology Laboratory, Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA, 24061, USA.
authors.forename: Rami A
authors.initials: RA
authors.lastname: Dalloul
authors.affiliation: College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University, Jinju, 52828, Republic of Korea. Electronic address: wongimin@gnu.ac.kr.
authors.forename: Wongi
authors.initials: W
authors.lastname: Min
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics / Q000378:metabolism*; D005075:Biological Evolution; D003001:Cloning, Molecular; D016207:Cytokines / Q000378:metabolism; D004372:Ducks / Q000276:immunology*; D045826:Flavobacteriaceae Infections / Q000276:immunology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D007113:Immunity, Innate; D018836:Inflammation Mediators / Q000378:metabolism; D064094:Interleukin-27 / Q000235:genetics / Q000378:metabolism*; D008214:Lymphocytes / Q000382:microbiology / Q000502:physiology*; D010802:Phylogeny; D060494:Riemerella / Q000276:immunology*; D013154:Spleen / Q000473:pathology; D019076:Transgenes / Q000235:genetics
chemical_list: D030161:Avian Proteins; D016207:Cytokines; D018836:Inflammation Mediators; D064094:Interleukin-27
keywords: Avian IL-17D; Ducks; Interleukin-17D; Riemerella infection