title: Analysis of the rDNA internal transcribed spacer region of theFusariumspecies by polymerase chain reaction-restriction fragment length polymorphism.
abstract: TheFusariumspecies are a widely spread phytopathogen identified in an extensive variety of hosts. TheFusariumgenus is one of the most heterogeneous fungi and is difficult to classify. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis is a useful method in detection of DNA polymorphism in objective sequences. The aim of the present study was to identify the phylogenetic associations and usefulness of the internal transcribed spacer (ITS) region as a genetic marker within the most clinically important strain of theFusariumspecies. A total of 50 strains ofFusariumspp. were used in the study, including environmental, clinical and reference isolates. The primers ITS1 and ITS4 were used in the study. Two restriction enzymes,HaeIII andSmaI, were assessed for the digestion of PCR products. A PCR product of ~550-base pairs was generated for eachFusariumspecies. The digested products withHaeIII andSmaI demonstrated that the bands generated for the medically significantFusariumspecies, includingF. solani,F. oxysporum,F. verticillidea,F. proliferatumandF. fujikuri, have different restriction enzyme patterns. In conclusion, it appears that the PCR-RFLP method used in the present study produces a sufficient restriction profile for differentiation of the most medically significantFusariumspecies.
authors.affiliation: Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Khuzestan 61357-15794, Iran; Department of Medical Mycology, Medical School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Khuzestan 61357-15794, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Zarrin
authors.affiliation: Department of Medical Mycology, Medical School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Khuzestan 61357-15794, Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Ganj
authors.affiliation: Department of Medical Mycology, Medical School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Khuzestan 61357-15794, Iran.
authors.forename: Sama
authors.initials: S
authors.lastname: Faramarzi
keywords: Fusarium; internal transcribed spacer region; polymerase chain reaction-restriction fragment length polymorphism