title: Illuminating Parasite Protein Production by Ribosome Profiling.
abstract: While technologies for global enumeration of transcript abundance are well-developed, those that assess protein abundance require tailoring to penetrate to low-abundance proteins. Ribosome profiling circumvents this challenge by measuring global protein production via sequencing small mRNA fragments protected by the assembled ribosome. This powerful approach is now being applied to protozoan parasites including trypanosomes and Plasmodium. It has been used to identify new protein-coding sequences (CDSs) and clarify the boundaries of previously annotated CDSs in Trypanosoma brucei. Ribosome profiling has demonstrated that translation efficiencies vary widely between genes and, for trypanosomes at least, for the same gene across stages. The ribosomal proteins are themselves subjected to translational control, suggesting a means of reinforcing global translational regulation.
authors.affiliation: Center for Infectious Disease Research (formerly Seattle Biomedical Research Institute), 307 Westlake Avenue North STE 500, Seattle, WA 98109 USA; Department of Global Health, Box 357965, University of Washington, Seattle, WA 98195, USA. Electronic address: marilyn.parsons@cidresearch.org.
authors.forename: Marilyn
authors.initials: M
authors.lastname: Parsons
authors.affiliation: Center for Infectious Disease Research (formerly Seattle Biomedical Research Institute), 307 Westlake Avenue North STE 500, Seattle, WA 98109 USA; Department of Global Health, Box 357965, University of Washington, Seattle, WA 98195, USA; Department of Biomedical Informatics and Medical Education, University of Washington, Seattle, WA 98195, USA.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Myler
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D023061:Gene Order; D018503:Genome, Protozoan / Q000235:genetics; D006790:Host-Parasite Interactions / Q000235:genetics*; D006801:Humans; D008018:Life Cycle Stages / Q000235:genetics; D040901:Proteomics; D012270:Ribosomes / Q000235:genetics*
keywords: Plasmodium; Trypanosoma; genome curation; ribosomal proteins; stage-regulation; translation