title: Voltammetric and spectrophotometric determination of antioxidant activity of Eugenia dysenterica DC leaves extracts.
abstract: Eugenia dysenterica DC (cagaiteira) is a native tree from Cerrado biome. Cagaita fruits are known and consumed in natura, mainly by inhabitants from Cerrado. In this study, we evaluated the antioxidant activity of leaves of this plant. For this evaluation we used four methods, the reduction of phosphomolybdenum, scanning by hydrogen peroxide, DPPH radical scavenging assay and determination of electrochemical parameters by differential pulse voltammetry. The results indicate that all extracts from leaves of this species have significant antioxidant potential, following the order: crude ethanol extract CEE) >crude aqueous extract (CAE) >crude hexane extract (CHE). The voltammetric approaches were also applied in order to evaluate the redox behavior of the hydrophilic extracts, as well as of their sub-extracts. Thus, the results suggest the presence of catechol-like polyphenols, which were confirmed by chromatography and phytochemical methods. Voltammetric analysis showed to be a reliable and fast method to determine redox behavior of E. dysenterica extracts.
authors.affiliation: Faculty of Ceilândia, University of Brasilia. Centro Metropolitano - Conjunto A - Lote 01, Ceilândia, Distrito Federal, Brazil.
authors.forename: Silva Elton
authors.initials: SE
authors.lastname: Clementino
authors.affiliation: Faculty of Pharmacy, Federal University of Goias. Av. Universitária, Setor Universitário, Goiânia, Goiás, Brazil.
authors.forename: Rezende Stefani
authors.initials: RS
authors.lastname: Garcia
authors.affiliation: Faculty of Ceilândia, University of Brasilia. Centro Metropolitano - Conjunto A - Lote 01, Ceilândia, Distrito Federal, Brazil.
authors.forename: Béda Roanna C Clícia
authors.initials: BR
authors.lastname: Moreira
authors.affiliation: Faculty of Ceilândia, University of Brasilia. Centro Metropolitano - Conjunto A - Lote 01, Ceilândia, Distrito Federal, Brazil.
authors.forename: Balest Aiessa
authors.initials: BA
authors.lastname: Pagliarini
authors.affiliation: Faculty of Ceilândia, University of Brasilia. Centro Metropolitano - Conjunto A - Lote 01, Ceilândia, Distrito Federal, Brazil.
authors.forename: Reis Bruna
authors.initials: RB
authors.lastname: Cabral
authors.affiliation: Laboratory of Natural Products, Faculty of Health Sciences, University of Brasilia. Campus Universitário Darcy Ribeiro, Asa Norte, Brasília, Distrito Federal, Brazil.
authors.forename: Silveira
authors.initials: S
authors.lastname: Dâmaris
authors.affiliation: Faculty of Pharmacy, Federal University of Goias. Av. Universitária, Setor Universitário, Goiânia, Goiás, Brazil.
authors.forename: Gil Eric
authors.initials: GE
authors.lastname: de Souza
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D002396:Catechols / Q000302:isolation & purification / Q000494:pharmacology*; D055664:Electrochemical Techniques; D000431:Ethanol / Q000737:chemistry; D000068856:Eugenia / Q000737:chemistry*; D006586:Hexanes / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D010084:Oxidation-Reduction; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves; D010946:Plants, Medicinal; D012997:Solvents / Q000737:chemistry; D013053:Spectrophotometry; D014867:Water / Q000737:chemistry
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D002396:Catechols; D006586:Hexanes; D010851:Picrates; D010936:Plant Extracts; D012997:Solvents; D014867:Water; D000431:Ethanol; C004931:1,1-diphenyl-2-picrylhydrazyl