title: MEKC-LIF method for analysis of amino acids after on-capillary derivatization by transverse diffusion of laminar flow profiles mixing of reactants for assessing developmental capacity of human embryos after in vitro fertilization.
abstract: Evaluating the physiological state of an organism is of clinical importance. In assisted reproduction, knowledge of the embryo's physiology is crucial for selecting the embryo with the highest developmental capacity to ensure high pregnancy rates. Amino acids (AAs) are involved in many biochemical processes during embryo development, which means that the determination of AA fluctuations in the embryo's surroundings can determine the embryo's physiological state. Since current embryo selection methods are mainly based on visual assessment, which lacks proper accuracy, a novel method for the analysis of AAs in the embryo's surroundings was developed. AAs were analyzed by means of MEKC-LIF after on-capillary derivatization by naphthalene-2,3-dicarboxaldehyde. The reactants were injected under the three zone arrangement and mixed using the transverse diffusion of laminar flow profiles methodology. The resulting derivatives of all the standard AAs were baseline resolved in the BGE comprised of 35 mM sodium tetraborate, 55 mM SDS, 2.7 M urea, 1 mM BIS-TRIS propane, and 23 mM NaOH within 50 min. The method was applied on an analysis of spent culture media used in assisted reproduction to culture embryos after in vitro fertilization.
authors.affiliation: Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice Brno, Czech Republic.
authors.forename: Andrea
authors.initials: A
authors.lastname: Celá
authors.affiliation: Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice Brno, Czech Republic.
authors.forename: Aleš
authors.initials: A
authors.lastname: Mádr
authors.affiliation: Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice Brno, Czech Republic.
authors.forename: Tereza
authors.initials: T
authors.lastname: Dědová
authors.affiliation: Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice Brno, Czech Republic.
authors.forename: Marta
authors.initials: M
authors.lastname: Pelcová
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine and University Hospital Brno, Obilní trh Brno, Czech Republic.
authors.forename: Michal
authors.initials: M
authors.lastname: Ješeta
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine and University Hospital Brno, Obilní trh Brno, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Žáková
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine and University Hospital Brno, Obilní trh Brno, Czech Republic.
authors.forename: Igor
authors.initials: I
authors.lastname: Crha
authors.affiliation: Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice Brno, Czech Republic. glatz@chemi.muni.cz.
authors.forename: Zdeněk
authors.initials: Z
authors.lastname: Glatz
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D047108:Embryonic Development; D005307:Fertilization in Vitro; D006801:Humans; D009281:Naphthalenes / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D000596:Amino Acids; D009281:Naphthalenes; C027717:2,3-naphthalenedicarboxaldehyde
keywords: Amino acids; Capillary electrophoresis; In vitro fertilization; On-capillary derivatization; Transverse diffusion of laminar flow profiles