title: Consolidated bioprocessing of poly(lactate-co-3-hydroxybutyrate) from xylan as a sole feedstock by genetically-engineered Escherichia coli.
abstract: Consolidated bioprocessing of lignocellulose is an attractive strategy for the sustainable production of petroleum-based alternatives. One of the underutilized sources of carbon in lignocellulose is the hemicellulosic fraction which largely consists of the polysaccharide xylan. In this study, Escherichia coli JW0885 (pyruvate formate lyase activator protein mutant, pflA(-)) was engineered to express recombinant xylanases and polyhydroxyalkanoate (PHA)-producing enzymes for the biosynthesis of poly(lactate-co-3-hydroxybutyrate) [P(LA-co-3HB)] from xylan as a consolidated bioprocess. The results show that E. coli JW0885 was capable of producing P(LA-co-3HB) when xylan was the only feedstock and different feeding and growth parameters were examined in order to improve upon initial yields. The highest yields of P(LA-co-3HB) copolymer obtained in this study occurred when xylan was added during mid-exponential growth after cells had been grown at high shaking-speeds (290 rpm). The results showed an inverse relationship between total PHA production and LA-monomer incorporation into the copolymer. Proton nuclear magnetic resonance ((1)H NMR), gel permeation chromatography (GPC), and differential scanning calorimetry (DSC) analyses corroborate that the polymers produced maintain physical properties characteristic of LA-incorporating PHB-based copolymers. The present study achieves the first ever engineering of a consolidated bioprocessing bacterial system for the production of a bioplastic from a hemicelluosic feedstock.
authors.affiliation: Department of Chemistry, State University of New York, College of Environmental Science and Forestry, Syracuse, NY 13210, USA.
authors.forename: Lucia
authors.initials: L
authors.lastname: Salamanca-Cardona
authors.affiliation: Department of Chemistry, State University of New York, College of Environmental Science and Forestry, Syracuse, NY 13210, USA.
authors.forename: Ryan A
authors.initials: RA
authors.lastname: Scheel
authors.affiliation: Department of Chemistry, State University of New York, College of Environmental Science and Forestry, Syracuse, NY 13210, USA.
authors.forename: Norman Scott
authors.initials: NS
authors.lastname: Bergey
authors.affiliation: Department of Chemistry, State University of New York, College of Environmental Science and Forestry, Syracuse, NY 13210, USA.
authors.forename: Arthur J
authors.initials: AJ
authors.lastname: Stipanovic
authors.affiliation: Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13-28, Kita-ku, Sapporo 060-8638, Japan.
authors.forename: Ken'ichiro
authors.initials: K
authors.lastname: Matsumoto
authors.affiliation: Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13-28, Kita-ku, Sapporo 060-8638, Japan; CREST, JST, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Taguchi
authors.affiliation: Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan 430062, People's Republic of China; Department of Chemistry, State University of New York, College of Environmental Science and Forestry, Syracuse, NY 13210, USA; Center for Applied Microbiology, State University of New York, College of Environmental Science and Forestry, Syracuse, NY 13210, USA. Electronic address: ctnomura@esf.edu.
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Nomura
mesh_terms: D001704:Biopolymers / Q000096:biosynthesis / Q000378:metabolism; D002152:Calorimetry, Differential Scanning; D002850:Chromatography, Gel; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics; D005818:Genetic Engineering; D011091:Polyesters / Q000378:metabolism*; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D066244:Proton Magnetic Resonance Spectroscopy; D014990:Xylans / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D001704:Biopolymers; D029968:Escherichia coli Proteins; D011091:Polyesters; D054813:Polyhydroxyalkanoates; D011134:Polysaccharides; D014990:Xylans; C000616599:poly(lactate-co-3-hydroxybutyrate); C007916:hemicellulose; D043364:Endo-1,4-beta Xylanases
keywords: Biomass; Consolidated bioprocessing; Hemicellulose; Polyhydroxyalkanoates; Recombinant Escherichia coli; Xylan; Xylanases