title: Screening and isolation of potential lactate dehydrogenase inhibitors from five Chinese medicinal herbs: Soybean, Radix pueraria, Flos pueraria, Rhizoma belamcandae, and Radix astragali.
abstract: Stroke is among the leading causes of death and severe disability worldwide. Flavonoids have been extensively used in the treatment of ischemic stroke by reducing lactate dehydrogenase levels and thereby enhancing blood perfusion to the ischemic region. Here, we used ultrafiltration high-performance liquid chromatography coupled with diode array detection and mass spectrometry for the rapid screening and identification of flavonoids from five Chinese medicinal herbs: soybean, Radix pueraria, Flos pueraria, Rhizoma belamcandae, and Radix astragali. Using PC12 cells as a suitable in vitro model of toxicity, cell viability was quantitated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The results showed that the extracts of soybean and the six major components, namely, acetyldaidzin, malonylgenistin, daidiain, glycitin, genistin, and acetylcitin; the extract of R. pueraria and its main component daidzein; the extract of F. pueraria and its three major components, tectorigenin, tectoridin, and tectorigenin-7-O-xylosylglucosid; and the extract of R. belamcandae and its main component, tectoridin, were strong lactate dehydrogenase inhibitors. Also, the components of R. astragali showed no bioactivity. These findings indicate that the ultrafltration high-performance liquid chromatography coupled with diode array detection and mass spectrometry method could be utilized in rapid screening and separation of bioactive compounds from a complex matrix.
authors.affiliation: Central Laboratory, Changchun Normal University, Erdao District, Changchun, Jilin, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Central Laboratory, Changchun Normal University, Erdao District, Changchun, Jilin, China.
authors.forename: Senlin
authors.initials: S
authors.lastname: Li
authors.affiliation: Central Laboratory, Changchun Normal University, Erdao District, Changchun, Jilin, China.
authors.forename: Sainan
authors.initials: S
authors.lastname: Li
authors.affiliation: Central Laboratory, Changchun Normal University, Erdao District, Changchun, Jilin, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Yang
authors.affiliation: Central Laboratory, Changchun Normal University, Erdao District, Changchun, Jilin, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Central Laboratory, Changchun Normal University, Erdao District, Changchun, Jilin, China.
authors.forename: Yuchi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Central Laboratory, Changchun Normal University, Erdao District, Changchun, Jilin, China.
authors.forename: Chunming
authors.initials: C
authors.lastname: Liu
mesh_terms: D000818:Animals; D027885:Astragalus propinquus; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008516:Medicine, Chinese Traditional; D016716:PC12 Cells; D051381:Rats; D013025:Glycine max / Q000737:chemistry*; D013329:Structure-Activity Relationship
chemical_list: D004365:Drugs, Chinese Herbal; D004791:Enzyme Inhibitors; C027492:Huang Qi; D007770:L-Lactate Dehydrogenase
keywords: High-performance liquid chromatography; Lactate dehydrogenase; PC12 cells; Stroke; Ultrafiltration