title: Cox26 is a novel stoichiometric subunit of the yeast cytochrome c oxidase.
abstract: The cytochrome c oxidase (COX) is the terminal enzyme of the respiratory chain. The complex accepts electrons from cytochrome c and passes them onto molecular oxygen. This process contributes to energy capture in the form of a membrane potential across the inner membrane. The enzyme complex assembles in a stepwise process from the three mitochondria-encoded core subunits Cox1, Cox2 and Cox3, which associate with nuclear-encoded subunits and cofactors. In the yeast Saccharomyces cerevisiae, the cytochrome c oxidase associates with the bc1-complex into supercomplexes, allowing efficient energy transduction. Here we report on Cox26 as a protein found in respiratory chain supercomplexes containing cytochrome c oxidase. Our analyses reveal Cox26 as a novel stoichiometric structural subunit of the cytochrome c oxidase. A loss of Cox26 affects cytochrome c oxidase activity and respirasome organization.
authors.affiliation: Department of Cellular Biochemistry, University Medical Center Göttingen, D-37073 Göttingen, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Levchenko
authors.affiliation: Department of Cellular Biochemistry, University Medical Center Göttingen, D-37073 Göttingen, Germany.
authors.forename: Jan-Moritz
authors.initials: JM
authors.lastname: Wuttke
authors.affiliation: Department of Cellular Biochemistry, University Medical Center Göttingen, D-37073 Göttingen, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Römpler
authors.affiliation: Department of Cellular Biochemistry, University Medical Center Göttingen, D-37073 Göttingen, Germany.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Schmidt
authors.affiliation: Department of Cellular Biochemistry, University Medical Center Göttingen, D-37073 Göttingen, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Neifer
authors.affiliation: Department of Cellular Biochemistry, University Medical Center Göttingen, D-37073 Göttingen, Germany.
authors.forename: Lisa
authors.initials: L
authors.lastname: Juris
authors.affiliation: Department of Cellular Biochemistry, University Medical Center Göttingen, D-37073 Göttingen, Germany.
authors.forename: Mirjam
authors.initials: M
authors.lastname: Wissel
authors.affiliation: Department of Cellular Biochemistry, University Medical Center Göttingen, D-37073 Göttingen, Germany; Max Planck Institute for Biophysical Chemistry, D-37077 Göttingen, Germany. Electronic address: peter.rehling@medizin.uni-goettingen.de.
authors.forename: Peter
authors.initials: P
authors.lastname: Rehling
authors.affiliation: Department of Cellular Biochemistry, University Medical Center Göttingen, D-37073 Göttingen, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Deckers
mesh_terms: D003576:Electron Transport Complex IV / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000201:enzymology*; D051336:Mitochondrial Membranes / Q000201:enzymology*; D010101:Oxygen Consumption; D021122:Protein Subunits; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013997:Time Factors
chemical_list: C000610448:Cox26 protein, S cerevisiae; D021122:Protein Subunits; D029701:Saccharomyces cerevisiae Proteins; D003576:Electron Transport Complex IV