title: How Chemical Synthesis of Ubiquitin Conjugates Helps To Understand Ubiquitin Signal Transduction.
abstract: Ubiquitin (Ub) is a small post-translational modifier protein involved in a myriad of biochemical processes including DNA damage repair, proteasomal proteolysis, and cell cycle control. Ubiquitin signaling pathways have not been completely deciphered due to the complex nature of the enzymes involved in ubiquitin conjugation and deconjugation. Hence, probes and assay reagents are important to get a better understanding of this pathway. Recently, improvements have been made in synthesis procedures of Ub derivatives. In this perspective, we explain various research reagents available and how chemical synthesis has made an important contribution to Ub research.
authors.affiliation: Division of Cell Biology II, The Netherlands Cancer Institute , Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.
authors.forename: Dharjath S
authors.initials: DS
authors.lastname: Hameed
authors.affiliation: Division of Cell Biology II, The Netherlands Cancer Institute , Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.
authors.forename: Aysegul
authors.initials: A
authors.lastname: Sapmaz
authors.affiliation: Division of Cell Biology II, The Netherlands Cancer Institute , Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.|Department of Chemical Immunology, Leiden University Medical Center , Einthovenweg 20, 2333 ZC Leiden, The Netherlands.
authors.forename: Huib
authors.initials: H
authors.lastname: Ovaa
mesh_terms: D000818:Animals; D060326:Chemistry Techniques, Synthetic / Q000379:methods; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D015398:Signal Transduction; D025801:Ubiquitin / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D057149:Ubiquitinated Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D015335:Molecular Probes; D010455:Peptides; D025801:Ubiquitin; D057149:Ubiquitinated Proteins