title: Epigenetic Profiling of H3K4Me3 Reveals Herbal Medicine Jinfukang-Induced Epigenetic Alteration Is Involved in Anti-Lung Cancer Activity.
abstract: Traditional Chinese medicine Jinfukang (JFK) has been clinically used for treating lung cancer. To examine whether epigenetic modifications are involved in its anticancer activity, we performed a global profiling analysis of H3K4Me3, an epigenomic marker associated with active gene expression, in JFK-treated lung cancer cells. We identified 11,670 genes with significantly altered status of H3K4Me3 modification following JFK treatment (P < 0.05). Gene Ontology analysis indicates that these genes are involved in tumor-related pathways, including pathway in cancer, basal cell carcinoma, apoptosis, induction of programmed cell death, regulation of transcription (DNA-templated), intracellular signal transduction, and regulation of peptidase activity. In particular, we found that the levels of H3K4Me3 at the promoters of SUSD2, CCND2, BCL2A1, and TMEM158 are significantly altered in A549, NCI-H1975, NCI-H1650, and NCI-H2228 cells, when treated with JFK. Collectively, these findings provide the first evidence that the anticancer activity of JFK involves modulation of histone modification at many cancer-related gene loci.
authors.affiliation: Shanghai Center for Systems Biomedicine, School of Biomedical Engineering, State Key Laboratory on Oncogene and Bio-ID Center, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lu
authors.affiliation: Shanghai Center for Systems Biomedicine, School of Biomedical Engineering, State Key Laboratory on Oncogene and Bio-ID Center, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Shanghai Center for Systems Biomedicine, School of Biomedical Engineering, State Key Laboratory on Oncogene and Bio-ID Center, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Shen
authors.affiliation: Tumor Institute of Traditional Chinese Medicine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 725 South Wanping Road, Shanghai 200032, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
authors.affiliation: Shanghai Center for Systems Biomedicine, School of Biomedical Engineering, State Key Laboratory on Oncogene and Bio-ID Center, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.affiliation: Shanghai Center for Systems Biomedicine, School of Biomedical Engineering, State Key Laboratory on Oncogene and Bio-ID Center, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
authors.forename: Hualei
authors.initials: H
authors.lastname: Kong
authors.affiliation: College of Life Science, Northwest University, 229 Taibai Road, Xi'an 710069, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Tian
authors.affiliation: Shanghai Center for Systems Biomedicine, School of Biomedical Engineering, State Key Laboratory on Oncogene and Bio-ID Center, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Shao
authors.affiliation: Shanghai Center for Systems Biomedicine, School of Biomedical Engineering, State Key Laboratory on Oncogene and Bio-ID Center, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China; Tumor Institute of Traditional Chinese Medicine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 725 South Wanping Road, Shanghai 200032, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Tumor Institute of Traditional Chinese Medicine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 725 South Wanping Road, Shanghai 200032, China; Department of Oncology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, 110 Ganhe Road, Shanghai 200437, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Xu