title: A novel amperometric catechol biosensor based on α-Fe2O3nanocrystals-modified carbon paste electrode.
abstract: In this work, we designed an amperometric catechol biosensor based on α-Fe2O3nanocrystals (NCs) incorporated carbon-paste electrode. Laccase enzyme is then assembled onto the modified electrode surface to form a nanobiocomposite enhancing the electron transfer reactions at the enzyme's active metal centers for catechol oxidation. The biosensor gave good sensitivity with a linear detection response in the range of 8-800 μM with limit of detection 4.28 μM. We successfully employed the sensor for real water sample analysis. The results illustrate that the metal oxide NCs have enormous potential in the construction of biosensors for sensitive determination of phenol derivatives.
authors.affiliation: a Department of Chemistry , CMR Institute of Technology , Bangalore , India.
authors.forename: C
authors.initials: C
authors.lastname: Sarika
authors.affiliation: b Department of Chemistry , ACS College of Engineering , Bangalore , India.
authors.forename: M S
authors.initials: MS
authors.lastname: Shivakumar
authors.affiliation: c Solid State & Structural Chemistry Unit , Indian Institute of Science , Bangalore , India.
authors.forename: C
authors.initials: C
authors.lastname: Shivakumara
authors.affiliation: d Department of Studies in Chemistry , Bangalore University , Bangalore , India.
authors.forename: G
authors.initials: G
authors.lastname: Krishnamurthy
authors.affiliation: a Department of Chemistry , CMR Institute of Technology , Bangalore , India.
authors.forename: B
authors.initials: B
authors.lastname: Narasimha Murthy
authors.affiliation: a Department of Chemistry , CMR Institute of Technology , Bangalore , India.
authors.forename: I C
authors.initials: IC
authors.lastname: Lekshmi
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D002396:Catechols / Q000032:analysis*; D055664:Electrochemical Techniques; D004566:Electrodes; D004579:Electron Transport; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000302:isolation & purification; D005290:Ferric Compounds / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D042845:Laccase / Q000737:chemistry / Q000302:isolation & purification; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D010084:Oxidation-Reduction; D055454:Trametes / Q000737:chemistry / Q000201:enzymology; D014867:Water / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D002396:Catechols; D004800:Enzymes, Immobilized; D005290:Ferric Compounds; D005656:Fungal Proteins; D014874:Water Pollutants, Chemical; D014867:Water; C000499:ferric oxide; D002244:Carbon; D042845:Laccase
keywords: Amperometric biosensing; enzyme; metal-oxide nanocrystals; phenolic detection