title: Opium induces apoptosis in Jurkat cells via promotion of pro-apoptotic and inhibition of anti-apoptotic molecules.
abstract: OBJECTIVE
  The aim of this study was to determine the important molecules involved in apoptosis induction by opium in Jurkat cell line.
  
  
  METHODS
  Jurkat cells were incubated 48 hrs with 2.86×10(-5) g/ml concentration of opium and apoptosis as well as expression levels of related molecules were measured.
  
  
  RESULTS
  Our results demonstrated that 50.3±0.2 percent of opium treated Jurkat cells were revealed apoptotic features. The levels of mRNA of several pro-apoptotic and anti-apoptotic molecules were increased and decreased, respectively, in the opium treated cells. The results also demonstrated that expression levels of BCL2, DFFA and NOL3 as anti-apoptotic molecules were increased in the opium treated cells.
  
  
  CONCLUSIONS
  It seems that opium induces apoptosis in Jurkat cells via both intrinsic and extrinsic pathways. Although opium induces apoptosis in the cells but increased expression of some anti-apoptotic molecules may be a normal resistance of the cell for death.
authors.affiliation: Department of Laboratoty Sciences, Faculty of Paramedicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
authors.forename: Mohammad Kazemi
authors.initials: MK
authors.lastname: Arababadi
authors.affiliation: Endocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Sciences and Department of Biochemistry, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Asadikaram
keywords: Apoptosis; Jurkat cells; PCR array