title: Increased levels of urinary biomarkers of lipid peroxidation products among workers occupationally exposed to diesel engine exhaust.
abstract: Diesel engine exhaust (DEE) was found to induce lipid peroxidation (LPO) in animal exposure studies. LPO is a class of oxidative stress and can be reflected by detecting the levels of its production, such as malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), and etheno-DNA adducts including 1,N(6)-etheno-2'-deoxyadenosine (ɛdA) and 3,N(4)-etheno-2'-deoxycytidine (ɛdC). However, the impact of DEE exposure on LPO has not been explored in humans. In this study, we evaluated urinary MDA, 4-HNE, ɛdA, and ɛdC levels as biomarkers of LPO among 108 workers with exclusive exposure to DEE and 109 non-DEE-exposed workers. Results showed that increased levels of urinary MDA and ɛdA were observed in subjects occupationally exposed to DEE before and after age, body mass index (BMI), smoking status, and alcohol use were adjusted (all p < 0.001). There was a statistically significant relationship between the internal exposure dose (urinary ΣOH-PAHs) and MDA, 4-HNE, and ɛdA (all p < 0.001). Furthermore, significant increased relations between urinary etheno-DNA adduct and MDA, 4-HNE were observed (all p < 0.05). The findings of this study suggested that the level of LPO products (MDA and ɛdA) was increased in DEE-exposed workers, and urinary MDA and ɛdA might be feasible biomarkers for DEE exposure. LPO induced DNA damage might be involved and further motivated the genomic instability could be one of the pathogeneses of cancer induced by DEE-exposure. However, additional investigations should be performed to understand these observations.
authors.affiliation: a Key Laboratory of Chemical Safety and Health , National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention , Beijing , China ;
authors.forename: Ping
authors.initials: P
authors.lastname: Bin
authors.affiliation: a Key Laboratory of Chemical Safety and Health , National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention , Beijing , China ;
authors.forename: Meili
authors.initials: M
authors.lastname: Shen
authors.affiliation: a Key Laboratory of Chemical Safety and Health , National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention , Beijing , China ;
authors.forename: Haibin
authors.initials: H
authors.lastname: Li
authors.affiliation: a Key Laboratory of Chemical Safety and Health , National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention , Beijing , China ;
authors.forename: Xin
authors.initials: X
authors.lastname: Sun
authors.affiliation: a Key Laboratory of Chemical Safety and Health , National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention , Beijing , China ;
authors.forename: Yong
authors.initials: Y
authors.lastname: Niu
authors.affiliation: a Key Laboratory of Chemical Safety and Health , National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention , Beijing , China ;
authors.forename: Tao
authors.initials: T
authors.lastname: Meng
authors.affiliation: a Key Laboratory of Chemical Safety and Health , National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention , Beijing , China ;
authors.forename: Tao
authors.initials: T
authors.lastname: Yu
authors.affiliation: a Key Laboratory of Chemical Safety and Health , National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention , Beijing , China ;
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhang
authors.affiliation: a Key Laboratory of Chemical Safety and Health , National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention , Beijing , China ;
authors.forename: Yufei
authors.initials: Y
authors.lastname: Dai
authors.affiliation: b Department of Environmental Toxicology , The Institute of Environmental and Human Health, Texas Tech University , Lubbock , TX , USA ;
authors.forename: Weimin
authors.initials: W
authors.lastname: Gao
authors.affiliation: c Henan Provincial Institute of Occupational Health , Zhengzhou , Henan , China.
authors.forename: Guizhen
authors.initials: G
authors.lastname: Gu
authors.affiliation: c Henan Provincial Institute of Occupational Health , Zhengzhou , Henan , China.
authors.forename: Shanfa
authors.initials: S
authors.lastname: Yu
authors.affiliation: a Key Laboratory of Chemical Safety and Health , National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention , Beijing , China ;
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000652:urine*; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation / Q000235:genetics*; D008297:Male; D016273:Occupational Exposure / Q000032:analysis; D001335:Vehicle Emissions / Q000032:analysis*
chemical_list: D015415:Biomarkers; D001335:Vehicle Emissions
keywords: Biomarkers; diesel engine exhaust; etheno-DNA adducts; lipid peroxidation; polycyclic aromatic hydrocarbons