title: Infection-Mediated Priming of Phagocytes Protects against Lethal Secondary Aspergillus fumigatus Challenge.
abstract: Phagocytes restrict the germination of Aspergillus fumigatus conidia and prevent the establishment of invasive pulmonary aspergillosis in immunecompetent mice. Here we report that immunecompetent mice recovering from a primary A. fumigatus challenge are protected against a secondary lethal challenge. Using RAGγc knock-out mice we show that this protection is independent of T, B and NK cells. In protected mice, lung phagocytes are recruited more rapidly and are more efficient in conidial phagocytosis and killing. Protection was also associated with an enhanced expression of CXCR2 and Dectin-1 on bone marrow phagocytes. We also show that protective lung cytokine and chemokine responses are induced more rapidly and with enhanced dynamics in protected mice. Our findings support the hypothesis that following a first encounter with a non-lethal dose of A. fumigatus conidia, the innate immune system is primed and can mediate protection against a secondary lethal infection.
authors.affiliation: Institut Pasteur, Unité Cytokines & Inflammation, Paris, France.|Fungal Genetics and Biology, School of Life Sciences, University of Nottingham, Nottingham, United Kingdom.
authors.forename: Amélie
authors.initials: A
authors.lastname: Savers
authors.affiliation: Institut Pasteur, Unité Cytokines & Inflammation, Paris, France.
authors.forename: Orhan
authors.initials: O
authors.lastname: Rasid
authors.affiliation: INSERM UMR S1163 -Institut Imagine, Laboratoire d'Immunité Intestinale, Paris, France.
authors.forename: Marianna
authors.initials: M
authors.lastname: Parlato
authors.affiliation: Fungal Genetics and Biology, School of Life Sciences, University of Nottingham, Nottingham, United Kingdom.
authors.forename: Matthias
authors.initials: M
authors.lastname: Brock
authors.affiliation: Institut Pasteur, Unité Histopathologie Humaine et Modèles Animaux, Paris, France.
authors.forename: Gregory
authors.initials: G
authors.lastname: Jouvion
authors.affiliation: INSERM, UMR 7355, Immunologie Moléculaire, Institut de Transgénose, Université d'Orléans et Centre National de la Recherche Scientifique, Orléans, France.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Ryffel
authors.affiliation: Institut Pasteur, Unité Cytokines & Inflammation, Paris, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Cavaillon
authors.affiliation: Institut Pasteur, Lymphoid Tissue Development Unit, Paris, France.
authors.forename: Gerard
authors.initials: G
authors.lastname: Eberl
authors.affiliation: Institut Pasteur, Unité Cytokines & Inflammation, Paris, France.
authors.forename: Oumaïma
authors.initials: O
authors.lastname: Ibrahim-Granet
mesh_terms: D000818:Animals; D001228:Aspergillosis / Q000276:immunology* / Q000382:microbiology; D001232:Aspergillus fumigatus / Q000276:immunology* / Q000502:physiology; D001853:Bone Marrow / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D018925:Chemokines / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D060467:Disease Resistance / Q000276:immunology; D005434:Flow Cytometry; D054884:Host-Pathogen Interactions / Q000276:immunology; D037181:Lectins, C-Type / Q000276:immunology / Q000378:metabolism; D008168:Lung / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010586:Phagocytes / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D023063:Receptors, Interleukin-8B / Q000276:immunology / Q000378:metabolism; D013172:Spores, Fungal / Q000276:immunology* / Q000502:physiology; D000099181:Dectin-1
chemical_list: D018925:Chemokines; D016207:Cytokines; D037181:Lectins, C-Type; D023063:Receptors, Interleukin-8B; D000099181:Dectin-1