title: Determination of Febuxostat in Human Plasma Using RP-LC-UV Method.
abstract: A simple and sensitive bioanalytical high-performance liquid chromatographic method with ultraviolet detection was developed and validated for the quantification of febuxostat (FEB) in human plasma. Ketoprofen was used as an internal standard. The analytes were extracted from human plasma samples by precipitation with acetonitrile. The reconstituted samples were chromatographed on C18 Bondapack 10 µm, 250 × 4.6 mm, Waters Column (Ireland) by using a mixture of acetonitrile and 0.5% aqueous phosphoric acid (pH 3) (52 : 48, v/v) as the mobile phase. The chromatographic separation was isocratically performed at room temperature at a flow rate of 1.0 mL/min with UV detection at 315 nm. The method exhibited a linear dynamic range over 0.05-5.00 µg/mL FEB in human plasma. The lower limit of quantification was 0.05 µg/mL. The results of the intra- and interday precision and accuracy studies were within the acceptable limits. The validated method was successfully applied for the determination of FEB in human plasma samples for application in bioequivalence studies.
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, El-Kasr El-Aini Street, ET-11562 Cairo, Egypt Department of Pharmaceutical Chemistry, College of Pharmacy, Hail University, King Saudi of Arabia kareemchem99@yahoo.com.
authors.forename: Kareem M
authors.initials: KM
authors.lastname: Younes
authors.affiliation: Pharmaceutical Chemistry Department, Faculty of Pharmacy, Cairo University, El-Kasr El-Aini Street, ET-11562 Cairo, Egypt.
authors.forename: Ehab F
authors.initials: EF
authors.lastname: El-Kady
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, El-Kasr El-Aini Street, ET-11562 Cairo, Egypt.
authors.forename: Eman S
authors.initials: ES
authors.lastname: Elzanfaly
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D000069465:Febuxostat / Q000097:blood*; D006801:Humans; D007660:Ketoprofen / Q000032:analysis; D057230:Limit of Detection; D010949:Plasma / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D000069465:Febuxostat; D007660:Ketoprofen