title: Paper-Based Device for Colorimetric and Photoelectrochemical Quantification of the Flux of H2O2 Releasing from MCF-7 Cancer Cells.
abstract: In this work, a novel dual photoelectrochemical/colorimetric cyto-analysis format was first introduced into a microfluidic paper-based analytical device (μ-PAD) for synchronous sensitive and visual detection of H2O2 released from tumor cells based on an in situ hydroxyl radicals ((•)OH) cleaving DNA approach. The resulted μ-PAD offered an excellent platform for high-performance biosensing applications, which was constructed by a layer-by-layer modification of concanavalin A, graphene quantum dots (GQDs) labeled flower-like Au@Pd alloy nanoparticles (NPs) probe, and tumor cells on the surface of the vertically aligned bamboo like ZnO, which grows on a pyknotic Pt NPs modified paper working electrode (ZnO/Pt-PWE). It was the effective matching of energy levels between GQDs and ZnO levels that lead to the enhancement of the photocurrent response compared with the bare ZnO/Pt-PWE. After releasing H2O2, the DNA strand was cleaved by (•)OH generated under the synergistic catalysis of GQDs and Au@Pd alloy NPs and thus, reduced the photocurrent, resulting in a high sensitivity to H2O2 in aqueous solutions with a detection limit of 0.05 nmol observed, much lower than that in the previously reported method. The disengaged probe can result in catalytic chromogenic reaction of substrates, resulting in real-time imaging of H2O2 biological processes. Therefore, this work provided a truly low-cost, simple, and disposable μ-PAD for precise and visual detection of cellular H2O2, which had potential utility to cellular biology and pathophysiology.
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan , Jinan 250022, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan , Jinan 250022, P. R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, University of Jinan , Jinan 250022, P. R. China.
authors.forename: Lina
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan , Jinan 250022, P. R. China.|Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, University of Jinan , Jinan 250022, P. R. China.
authors.forename: Shenguang
authors.initials: S
authors.lastname: Ge
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan , Jinan 250022, P. R. China.
authors.forename: Haiyun
authors.initials: H
authors.lastname: Liu
authors.affiliation: School of Biological Science and Technology, University of Jinan , Jinan 250022, P. R. China.
authors.forename: Na
authors.initials: N
authors.lastname: Ren
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan , Jinan 250022, P. R. China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yan
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan , Jinan 250022, P. R. China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
mesh_terms: D000497:Alloys / Q000737:chemistry; D003124:Colorimetry; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis*; D017665:Hydroxyl Radical / Q000737:chemistry; D057230:Limit of Detection; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000737:chemistry; D010165:Palladium / Q000737:chemistry; D010209:Paper; D045663:Quantum Dots / Q000737:chemistry
chemical_list: D000497:Alloys; D017665:Hydroxyl Radical; D010165:Palladium; D006046:Gold; D006108:Graphite; D004247:DNA; D006861:Hydrogen Peroxide