title: Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy.
abstract: A novel synthesized nitroxide amide-BODIPY prefluorescent probe was used to study cellular redox balance that modulates nitroxide/hydroxylamine ratio in cultured human fibroblasts. FLIM quantitatively differentiated between nitroxide states of the cytoplasm-localized probe imaged by TIRF, monitoring nitroxide depletion by hydrogen peroxide; eluding incorrect interpretation if only fluorescence intensity is considered.
authors.affiliation: Instituto de Ciencia y Tecnología de Polímeros, Consejo Superior de Investigaciones Científicas (ICTP-CSIC), Juan de la Cierva 3, 28006, Madrid, España and Department of Chemistry and Biomolecular Sciences and Centre for Catalysis Research and Innovation, University of Ottawa, 10 Marie Curie, Ottawa, Ontario K1N6N5, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Liras
authors.affiliation: Department of Chemistry and Biomolecular Sciences and Centre for Catalysis Research and Innovation, University of Ottawa, 10 Marie Curie, Ottawa, Ontario K1N6N5, Canada and INQUIMAE and Departamento de Química Inorgánica, Analítica, y Química Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina.
authors.forename: S
authors.initials: S
authors.lastname: Simoncelli
authors.affiliation: Laboratorio de Radiobiología Molecular y Celular, Departamento de Aplicaciones Nucleares, Comisión Chilena de Energía Nuclear, Santiago, Chile.
authors.forename: A
authors.initials: A
authors.lastname: Rivas-Aravena
authors.affiliation: Instituto de Ciencia y Tecnología de Polímeros, Consejo Superior de Investigaciones Científicas (ICTP-CSIC), Juan de la Cierva 3, 28006, Madrid, España.
authors.forename: O
authors.initials: O
authors.lastname: García
authors.affiliation: Department of Chemistry and Biomolecular Sciences and Centre for Catalysis Research and Innovation, University of Ottawa, 10 Marie Curie, Ottawa, Ontario K1N6N5, Canada.
authors.forename: J C
authors.initials: JC
authors.lastname: Scaiano
authors.affiliation: Department of Chemistry and Biomolecular Sciences and Centre for Catalysis Research and Innovation, University of Ottawa, 10 Marie Curie, Ottawa, Ontario K1N6N5, Canada and Bio-nanomaterials Chemistry and Engineering Laboratory, Division of Cardiac Surgery, University of Ottawa Heart Institute, 40 Ruskin St., Ottawa, Ontario K1Y 4W7, Canada. ealarcon@ottawa.heart.ca.
authors.forename: E I
authors.initials: EI
authors.lastname: Alarcon
authors.affiliation: Facultad de Química y Biología, Universidad de Santiago de Chile, Casilla 40 Correo 33, Santiago, Chile. alexis.aspee@usach.cl.
authors.forename: A
authors.initials: A
authors.lastname: Aspée
mesh_terms: D000577:Amides / Q000737:chemistry*; D001896:Boron Compounds / Q000737:chemistry*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D008856:Microscopy, Fluorescence; D009589:Nitrogen Oxides / Q000737:chemistry*; D010084:Oxidation-Reduction
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D000577:Amides; D001896:Boron Compounds; D005456:Fluorescent Dyes; D009589:Nitrogen Oxides; D006861:Hydrogen Peroxide