title: Three-dimensional rendering of otolith growth using phase contrast synchrotron tomography.
abstract: A three-dimensional computer reconstruction of a plaice Pleuronectes platessa otolith is presented from data acquired by the Diamond Light synchrotron, beamline I12, X-ray source, a high energy (53-150 keV) source particularly well suited to the study of dense objects. The data allowed non-destructive rendering of otolith structure, and for the first time allows otolith annuli (internal ring structures) to be analysed in X-ray tomographic images.
authors.affiliation: School of Computing Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, U.K.
authors.forename: J J I
authors.initials: JJ
authors.lastname: Mapp
authors.affiliation: School of Computing Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, U.K.
authors.forename: M H
authors.initials: MH
authors.lastname: Fisher
authors.affiliation: Diamond Light Source Ltd., Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, U.K.
authors.forename: R C
authors.initials: RC
authors.lastname: Atwood
authors.affiliation: Faculty of Health and Science, University Campus Suffolk, Waterfront Building, Neptune Quay, Ipswich, IP4 1QJ, U.K.
authors.forename: G D
authors.initials: GD
authors.lastname: Bell
authors.affiliation: Faculty of Science, School of Dentistry and Health Sciences, Charles Sturt University, Panorama Avenue, Bathurst, NSW, 2795, Australia.|University of Bath Bee Unit, Department of Biology and Biochemistry, Faculty of Sciences, University of Bath, BA2 7AY, U.K.
authors.forename: M K
authors.initials: MK
authors.lastname: Greco
authors.affiliation: Centre for Environment, Fisheries and Aquaculture Science, Pakefield Road, Lowestoft, Suffolk, NR33 0HT, U.K.
authors.forename: S
authors.initials: S
authors.lastname: Songer
authors.affiliation: Centre for Environment, Fisheries and Aquaculture Science, Pakefield Road, Lowestoft, Suffolk, NR33 0HT, U.K.
authors.forename: E
authors.initials: E
authors.lastname: Hunter
mesh_terms: D000818:Animals; D005399:Fishes / Q000254:growth & development*; D021621:Imaging, Three-Dimensional; D010037:Otolithic Membrane / Q000000981:diagnostic imaging* / Q000254:growth & development; D017356:Synchrotrons; D014054:Tomography; D014057:Tomography, X-Ray Computed / Q000379:methods
keywords: annuli; fish ageing; non-destructive sampling