title: MicroRNA-148b is a potential prognostic biomarker and predictor of response to radiotherapy in non-small-cell lung cancer.
abstract: miR-148b has been found to be aberrantly expressed in various tumor types. It has recently been reported to be involved in regulating radioresistance in non-small cell lung cancer (NSCLC) cells. However, its expression level and association with radiosensitivity in human patient samples have not been investigated. Real-time PCR was used to evaluate the expression levels of miR-148b. Χ (2) test was performed to analyze the association between miR-148b expression levels and clinicopathological factors or radiosensitivity. Kaplan-Meier survival curve was constructed to estimate the overall survival (OS), and the differences in survival were compared using the log-rank test. Cox regression analysis was conducted to determine the prognostic value of miR-148b. The relative level of miR-148b was significantly decreased in NSCLC tissues compared with matched non-cancerous tissues (P < 0.0001), and it was higher in tissues of patients who are good responders compared to those who are poor responders to radiotherapy (P < 0.0001). Lower expression of miR-148b was positively associated with high tumor stage (P = 0.0407) and radioresistance (P = 0.0002), and it predicted poor survival in patients with (P = 0.0129) or without (P = 0.0094) radiotherapy treatment. miR-148b was an independent prognostic factor for NSCLC as demonstrated by Cox proportional hazards risk analysis. miR-148b may serve as a prognostic biomarker of poor survival and a novel predictor of response to radiotherapy treatment in NSCLC.
authors.affiliation: Department of Thoracic Surgery, The First Hospital of Shijiazhuang, 36 Fanxi Road, Chang'an District, Shijiazhuang, 050011, Hebei Province, China.
authors.forename: Renfeng
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Hyperbaric Medicine, The Third Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei Province, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Ye
authors.affiliation: Department of Thoracic Surgery, The First Hospital of Shijiazhuang, 36 Fanxi Road, Chang'an District, Shijiazhuang, 050011, Hebei Province, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhen
authors.affiliation: Department of Anesthesiology, The First Hospital of Shijiazhuang, 36 Fanxi Road, Chang'an District, Shijiazhuang, 050011, Hebei Province, China.
authors.forename: Tieying
authors.initials: T
authors.lastname: Song
authors.affiliation: Department of Thoracic Surgery, The First Hospital of Shijiazhuang, 36 Fanxi Road, Chang'an District, Shijiazhuang, 050011, Hebei Province, China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Tan
authors.affiliation: Department of Thoracic Surgery, The First Hospital of Shijiazhuang, 36 Fanxi Road, Chang'an District, Shijiazhuang, 050011, Hebei Province, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Chu
authors.affiliation: Department of Thoracic Surgery, The First Hospital of Shijiazhuang, 36 Fanxi Road, Chang'an District, Shijiazhuang, 050011, Hebei Province, China.
authors.forename: Yaxiao
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Thoracic Surgery, The First Hospital of Shijiazhuang, 36 Fanxi Road, Chang'an District, Shijiazhuang, 050011, Hebei Province, China.
authors.forename: Baolei
authors.initials: B
authors.lastname: Lv
authors.affiliation: Department of Thoracic Surgery, The First Hospital of Shijiazhuang, 36 Fanxi Road, Chang'an District, Shijiazhuang, 050011, Hebei Province, China.
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Thoracic Surgery, The First Hospital of Shijiazhuang, 36 Fanxi Road, Chang'an District, Shijiazhuang, 050011, Hebei Province, China. jiabaoliu4@163.com.
authors.forename: Jiabao
authors.initials: J
authors.lastname: Liu
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy*; D016009:Chi-Square Distribution; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects*; D006801:Humans; D008168:Lung / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D008175:Lung Neoplasms / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy*; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis; D012334:RNA, Neoplasm / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D016019:Survival Analysis
chemical_list: D015415:Biomarkers; C550570:MIRN148 microRNA, human; D035683:MicroRNAs; D012334:RNA, Neoplasm
keywords: NSCLC; Prognosis; Radioresistance; miR-148b