title: Enzyme Shielding in an Enzyme-thin and Soft Organosilica Layer.
abstract: The fragile nature of most enzymes is a major hindrance to their use in industrial processes. Herein, we describe a synthetic chemical strategy to produce hybrid organic/inorganic nanobiocatalysts; it exploits the self-assembly of silane building blocks at the surface of enzymes to grow an organosilica layer, of controlled thickness, that fully shields the enzyme. Remarkably, the enzyme triggers a rearrangement of this organosilica layer into a significantly soft structure. We demonstrate that this change in stiffness correlates with the biocatalytic turnover rate, and that the organosilica layer shields the enzyme in a soft environment with a markedly enhanced resistance to denaturing stresses.
authors.affiliation: School of Life Science, University of Applied Sciences and Arts Northwestern Switzerland, Gründenstrasse 40, 4132, Muttenz, Switzerland.
authors.forename: M Rita
authors.initials: MR
authors.lastname: Correro
authors.affiliation: School of Life Science, University of Applied Sciences and Arts Northwestern Switzerland, Gründenstrasse 40, 4132, Muttenz, Switzerland.
authors.forename: Negar
authors.initials: N
authors.lastname: Moridi
authors.affiliation: School of Life Science, University of Applied Sciences and Arts Northwestern Switzerland, Gründenstrasse 40, 4132, Muttenz, Switzerland.|INOFEA AG, Hochbergerstrasse 60C, 4057, Basel, Switzerland.
authors.forename: Hansjörg
authors.initials: H
authors.lastname: Schützinger
authors.affiliation: School of Life Science, University of Applied Sciences and Arts Northwestern Switzerland, Gründenstrasse 40, 4132, Muttenz, Switzerland.
authors.forename: Sabine
authors.initials: S
authors.lastname: Sykora
authors.affiliation: School of Life Science, University of Applied Sciences and Arts Northwestern Switzerland, Gründenstrasse 40, 4132, Muttenz, Switzerland.
authors.forename: Erik M
authors.initials: EM
authors.lastname: Ammann
authors.affiliation: School of Life Science, University of Applied Sciences and Arts Northwestern Switzerland, Gründenstrasse 40, 4132, Muttenz, Switzerland.
authors.forename: E Henrik
authors.initials: EH
authors.lastname: Peters
authors.affiliation: INOFEA AG, Hochbergerstrasse 60C, 4057, Basel, Switzerland.
authors.forename: Yves
authors.initials: Y
authors.lastname: Dudal
authors.affiliation: School of Life Science, University of Applied Sciences and Arts Northwestern Switzerland, Gründenstrasse 40, 4132, Muttenz, Switzerland.|School of the Environment, Nanjing University, 210093, Nanjing, China.
authors.forename: Philippe F-X
authors.initials: PF
authors.lastname: Corvini
authors.affiliation: School of Life Science, University of Applied Sciences and Arts Northwestern Switzerland, Gründenstrasse 40, 4132, Muttenz, Switzerland. patrick.shahgaldian@fhnw.ch.
authors.forename: Patrick
authors.identifier: 0000-0002-1743-0387
authors.initials: P
authors.lastname: Shahgaldian
mesh_terms: D000135:Acid Phosphatase / Q000737:chemistry / Q000378:metabolism; D055162:Biocatalysis; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005696:Galactosidases / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry; D017646:Organosilicon Compounds / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D004798:Enzymes; D004800:Enzymes, Immobilized; D017646:Organosilicon Compounds; D012822:Silicon Dioxide; D000135:Acid Phosphatase; D005696:Galactosidases
keywords: enzyme catalysis; nanobiocatalysts; nanoparticles; organosilica; self-assembly