title: Unique haplotypes of cacao trees as revealed by trnH-psbA chloroplast DNA.
abstract: Cacao trees have been cultivated in Mesoamerica for at least 4,000 years. In this study, we analyzed sequence variation in the chloroplast DNA trnH-psbA intergenic spacer from 28 cacao trees from different farms in the Soconusco region in southern Mexico. Genetic relationships were established by two analysis approaches based on geographic origin (five populations) and genetic origin (based on a previous study). We identified six polymorphic sites, including five insertion/deletion (indels) types and one transversion. The overall nucleotide diversity was low for both approaches (geographic = 0.0032 and genetic = 0.0038). Conversely, we obtained moderate to high haplotype diversity (0.66 and 0.80) with 10 and 12 haplotypes, respectively. The common haplotype (H1) for both networks included cacao trees from all geographic locations (geographic approach) and four genetic groups (genetic approach). This common haplotype (ancient) derived a set of intermediate haplotypes and singletons interconnected by one or two mutational steps, which suggested directional selection and event purification from the expansion of narrow populations. Cacao trees from Soconusco region were grouped into one cluster without any evidence of subclustering based on AMOVA (F ST = 0) and SAMOVA (F ST = 0.04393) results. One population (Mazatán) showed a high haplotype frequency; thus, this population could be considered an important reservoir of genetic material. The indels located in the trnH-psbA intergenic spacer of cacao trees could be useful as markers for the development of DNA barcoding.
authors.affiliation: Instituto de Biociencias, Universidad Autónoma de Chiapas , Tapachula, Chiapas , Mexico.
authors.forename: Nidia
authors.initials: N
authors.lastname: Gutiérrez-López
authors.affiliation: Instituto de Biociencias, Universidad Autónoma de Chiapas , Tapachula, Chiapas , Mexico.
authors.forename: Isidro
authors.initials: I
authors.lastname: Ovando-Medina
authors.affiliation: Instituto de Biociencias, Universidad Autónoma de Chiapas , Tapachula, Chiapas , Mexico.
authors.forename: Miguel
authors.initials: M
authors.lastname: Salvador-Figueroa
authors.affiliation: Departamento de Ecología de la Biodiversidad, Instituto de Ecología, Universidad Nacional Autónoma de México , Hermosillo, Sonora , Mexico.
authors.forename: Francisco
authors.initials: F
authors.lastname: Molina-Freaner
authors.affiliation: Campo Experimental Rosario Izapa, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias , Tuxtla Chico, Chiapas , Mexico.
authors.forename: Carlos H
authors.initials: CH
authors.lastname: Avendaño-Arrazate
authors.affiliation: Instituto de Biociencias, Universidad Autónoma de Chiapas , Tapachula, Chiapas , Mexico.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Vázquez-Ovando
keywords: Chloroplast DNA; Haplotype; Indels; Nucleotide diversity; trnH-psbA