title: Adaptation of pancreatic islet cyto-architecture during development.
abstract: Plasma glucose in mammals is regulated by hormones secreted by the islets of Langerhans embedded in the exocrine pancreas. Islets consist of endocrine cells, primarily α, β, and δ cells, which secrete glucagon, insulin, and somatostatin, respectively. β cells form irregular locally connected clusters within islets that act in concert to secrete insulin upon glucose stimulation. Varying demands and available nutrients during development produce changes in the local connectivity of β cells in an islet. We showed in earlier work that graph theory provides a framework for the quantification of the seemingly stochastic cyto-architecture of β cells in an islet. To quantify the dynamics of endocrine connectivity during development requires a framework for characterizing changes in the probability distribution on the space of possible graphs, essentially a Fokker-Planck formalism on graphs. With large-scale imaging data for hundreds of thousands of islets containing millions of cells from human specimens, we show that this dynamics can be determined quantitatively. Requiring that rearrangement and cell addition processes match the observed dynamic developmental changes in quantitative topological graph characteristics strongly constrained possible processes. Our results suggest that there is a transient shift in preferred connectivity for β cells between 1-35 weeks and 12-24 months.
authors.affiliation: Laboratory of Biological Modeling, NIDDK, NIH, Bethesda, MD, USA.
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Striegel
authors.forename: Manami
authors.initials: M
authors.lastname: Hara
authors.forename: Vipul
authors.initials: V
authors.lastname: Periwal
mesh_terms: D002452:Cell Count; D002675:Child, Preschool; D003196:Computer Graphics; D003198:Computer Simulation; D005934:Glucagon / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007328:Insulin / Q000032:analysis / Q000378:metabolism; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000378:metabolism; D007515:Islets of Langerhans / Q000166:cytology* / Q000254:growth & development* / Q000378:metabolism; D008954:Models, Biological; D013269:Stochastic Processes
chemical_list: D007328:Insulin; D005934:Glucagon