title: Thioether bond formation by SPASM domain radical SAM enzymes: Cα H-atom abstraction in subtilosin A biosynthesis.
abstract: AlbA is a radical SAM enzyme catalyzing the formation of three unusual thioether bonds in the antibiotic subtilosin A. We demonstrate here that AlbA catalyzes direct Cα H-atom abstraction and likely contains three essential [4Fe-4S] centers. This leads us to propose novel mechanistic perspectives for thioether bond catalysis by radical SAM enzymes.
authors.affiliation: Micalis Institute, ChemSyBio, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France. Olivier.Berteau@jouy.inra.fr.
authors.forename: Alhosna
authors.initials: A
authors.lastname: Benjdia
authors.affiliation: Micalis Institute, ChemSyBio, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France. Olivier.Berteau@jouy.inra.fr.
authors.forename: Alain
authors.initials: A
authors.lastname: Guillot
authors.affiliation: INSERM U982, F-76821 Mont-Saint-Aignan, France and Institute for Research and Innovation in Biomedicine (IRIB), Regional Platform for Cell Imaging, PRIMACEN, University of Rouen, F-76821 Mont-Saint-Aignan, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Lefranc
authors.affiliation: INSERM U982, F-76821 Mont-Saint-Aignan, France and Institute for Research and Innovation in Biomedicine (IRIB), Regional Platform for Cell Imaging, PRIMACEN, University of Rouen, F-76821 Mont-Saint-Aignan, France.
authors.forename: Hubert
authors.initials: H
authors.lastname: Vaudry
authors.affiliation: INSERM U982, F-76821 Mont-Saint-Aignan, France and Institute for Research and Innovation in Biomedicine (IRIB), Regional Platform for Cell Imaging, PRIMACEN, University of Rouen, F-76821 Mont-Saint-Aignan, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Leprince
authors.affiliation: Micalis Institute, ChemSyBio, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France. Olivier.Berteau@jouy.inra.fr.
authors.forename: Olivier
authors.initials: O
authors.lastname: Berteau
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D001430:Bacteriocins / Q000096:biosynthesis* / Q000737:chemistry; D055162:Biocatalysis; D005609:Free Radicals / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D010456:Peptides, Cyclic / Q000096:biosynthesis* / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001430:Bacteriocins; D005609:Free Radicals; D010456:Peptides, Cyclic; D013440:Sulfides; C047828:sboA protein, Bacillus subtilis