title: Insights into the Prunus-Specific S-RNase-Based Self-Incompatibility System from a Genome-Wide Analysis of the Evolutionary Radiation of S Locus-Related F-box Genes.
abstract: Self-incompatibility (SI) is an important plant reproduction mechanism that facilitates the maintenance of genetic diversity within species. Three plant families, the Solanaceae, Rosaceae and Plantaginaceae, share an S-RNase-based gametophytic SI (GSI) system that involves a single S-RNase as the pistil S determinant and several F-box genes as pollen S determinants that act via non-self-recognition. Previous evidence has suggested a specific self-recognition mechanism in Prunus (Rosaceae), raising questions about the generality of the S-RNase-based GSI system. We investigated the evolution of the pollen S determinant by comparing the sequences of the Prunus S haplotype-specific F-box gene (SFB) with those of its orthologs in other angiosperm genomes. Our results indicate that the Prunus SFB does not cluster with the pollen S of other plants and diverged early after the establishment of the Eudicots. Our results further indicate multiple F-box gene duplication events, specifically in the Rosaceae family, and suggest that the Prunus SFB gene originated in a recent Prunus-specific gene duplication event. Transcriptomic and evolutionary analyses of the Prunus S paralogs are consistent with the establishment of a Prunus-specific SI system, and the possibility of subfunctionalization differentiating the newly generated SFB from the original pollen S determinant.
authors.affiliation: Laboratory of Pomology, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502 Japan Department of Plant Biology and Genome Center, University of California Davis, Davis, CA 95616, USA.
authors.forename: Takashi
authors.initials: T
authors.lastname: Akagi
authors.affiliation: Department of Plant Biology and Genome Center, University of California Davis, Davis, CA 95616, USA.
authors.forename: Isabelle M
authors.initials: IM
authors.lastname: Henry
authors.affiliation: Laboratory of Pomology, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502 Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Morimoto
authors.affiliation: Laboratory of Pomology, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502 Japan rtao@kais.kyoto-u.ac.jp.
authors.forename: Ryutaro
authors.initials: R
authors.lastname: Tao
mesh_terms: D019143:Evolution, Molecular; D044783:F-Box Proteins / Q000235:genetics* / Q000378:metabolism; D020440:Gene Duplication / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D056426:Genetic Loci; D014644:Genetic Variation; D008957:Models, Genetic; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D027861:Prunus / Q000235:genetics* / Q000502:physiology*; D012260:Ribonucleases / Q000235:genetics*; D012641:Selection, Genetic; D060436:Self-Incompatibility in Flowering Plants / Q000235:genetics*; D013045:Species Specificity; D013997:Time Factors
chemical_list: D044783:F-Box Proteins; D010940:Plant Proteins; D012260:Ribonucleases; C022542:ribonuclease S
keywords: Evolutionary analysis; F-box gene; Gene duplication; Prunus; Rosaceae; S-RNase-based self-incompatibility