title: Measuring Redox Status of Melanoma Cells.
abstract: Redox homeostasis plays multiple roles in essentially all aspects of cellular function, and hence, reliable methods for measuring cellular or tissue redox status are key elements in understanding the redox related signal pathways. However, in the free radical biology field, there are many controversies on the methods to measure reactive oxygen species. In this chapter we describe our experience in measuring superoxide, hydrogen peroxide, and a general redox status using redox-sensitive green fluorescence proteins (roGFPs) in human melanoma cells.
authors.affiliation: Department of Epidemiology, Chao Family Comprehensive Cancer Center, University of California Irvine School of Medicine, B200 Sprague Hall, 839 Health Science Road, Irvine, CA, 92697, USA. liufe@uci.edu.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu-Smith
authors.affiliation: Beckman Laser Institute and Medical Clinic, University of California Irvine, Irvine, CA, 92697, USA.
authors.forename: Tatiana B
authors.initials: TB
authors.lastname: Krasieva
authors.affiliation: The State Key Laboratory of Medical Genetics and School of Life Sciences, Central South University, Changsha, Hunan Province, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Xi'an Jiaotong University School of Life Science and Technology, Xi'an, Shaangxi Province, China.
authors.forename: Jiankang
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Medicine, Chao Family Comprehensive Cancer Center, University of California Irvine School of Medicine, B200 Sprague Hall, 839 Health Science Road, Irvine, CA, 92697, USA.
authors.forename: Frank L
authors.initials: FL
authors.lastname: Meyskens
keywords: Dichlorofluorescin diacetate (DCFDA); Dihydroethidium (DHE); Fluorometer; Hydrogen peroxide; Laser microscopy; Reactive oxygen species; Redox-sensitive GFP; Superoxide; ro1GFP; ro2GFP