title: Enhancement of ganoderic acid production by constitutively expressing Vitreoscilla hemoglobin gene in Ganoderma lucidum.
abstract: The Vitreoscilla hemoglobin (VHb) gene was expressed in Ganoderma lucidum to enhance antitumor ganoderic acid (GA) production. The effects of VHb expression on the accumulation of GAs and lanosterol (intermediate) and the transcription of GA biosynthesis genes were also investigated. In VHb-expressing G. lucidum, the maximum concentrations of four individual GAs (GA-S, GA-T, GA-Mk and GA-Me) were 19.1±1.8, 34.6±2.1, 191.5±13.1 and 45.2±2.8μg/100mg dry weight, respectively, which were 1.4-, 2.2, 1.9- and 2.0-fold higher than those obtained in the wild-type strain. Moreover, the maximum lanosterol concentration in the strain expressing VHb was 1.28-fold lower than that in the wild-type strain. The transcription levels of 3-hydroxy-3-methylglutaryl coenzyme A reductase, squalene synthase, and lanosterol synthase genes were up-regulated by 1.6-, 1.5-, and 1.6-fold, respectively, in the strain expressing VHb. This work is beneficial in developing an efficient fermentation process for the hyperproduction of GAs.
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
authors.forename: Huan-Jun
authors.initials: HJ
authors.lastname: Li
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
authors.forename: Yi-Long
authors.initials: YL
authors.lastname: He
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
authors.forename: De-Huai
authors.initials: DH
authors.lastname: Zhang
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
authors.forename: Tong-Hui
authors.initials: TH
authors.lastname: Yue
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
authors.forename: Lu-Xi
authors.initials: LX
authors.lastname: Jiang
authors.affiliation: Faculty of Science, Kunming University of Science and Technology, Kunming, 6500500, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China. Electronic address: xjuwei@163.com.
authors.forename: Jun-Wei
authors.initials: JW
authors.lastname: Xu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D018533:Biomass; D053898:Biosynthetic Pathways / Q000235:genetics; D001709:Biotechnology / Q000379:methods*; D015870:Gene Expression; D007810:Lanosterol / Q000378:metabolism; D020940:Reishi / Q000235:genetics*; D013997:Time Factors; D014158:Transcription, Genetic; D014170:Transformation, Genetic; D014315:Triterpenes / Q000378:metabolism*; D054793:Truncated Hemoglobins / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D014315:Triterpenes; D054793:Truncated Hemoglobins; C556862:ganoderic acid; C082613:hemoglobin protein, Vitreoscilla; D007810:Lanosterol
keywords: Biosynthetic genes; Ganoderic acid productions; Medicinal mushroom fermentation; Vitreoscilla hemoglobin