title: Identification of α-tubulin, Der f 33, as a novel allergen from Dermatophagoides farinae.
abstract: House dust mites are an important source of indoor allergens. More than 30 allergens of Dermatophagoides farinae (D. farinae) have been identified. Yet there may be many other allergens in mites remain to be characterized.
  
  
  
  α-Tubulin (also named Der f 33) was cloned, expressed and purified. Reaction to specific-IgE, skin prick test and a mouse asthma model were employed to determine the allergenicity of Der f 33.
  
  
  
  The recombinant Der f 33 reacted to the serum of patients with mite allergy. The positive rate of skin prick test (SPT) was 23.5%. In an asthma mouse model, Der f 33 induced the airway allergy-like responses. Moreover, serum specific IgE and IgG1, interleukin-4 (IL-4) from bronchoalveolar lavage fluid (BALF) and spleen cell culture supernatant were markedly increased. In addition, Der f 33 upregulated the CD80 and TNF-α levels in dendritic cells (DCs).
  
  
  
  Der f 33 is a novel allergen of D. farinae. It modulates the functions of DCs and induces airway allergy.
authors.affiliation: Otolaryngology Institutes, Allergy and Immunology Research Center, Shenzhen University, Shenzhen 518060, China; The State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University; Shenzhen University School of Medicine, 3688 Nanhai Blvd., Shenzhen 518060, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Otolaryngology Institutes, Allergy and Immunology Research Center, Shenzhen University, Shenzhen 518060, China.
authors.forename: Jianli
authors.initials: J
authors.lastname: Lin
authors.affiliation: Otolaryngology Institutes, Allergy and Immunology Research Center, Shenzhen University, Shenzhen 518060, China; The State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University; Shenzhen University School of Medicine, 3688 Nanhai Blvd., Shenzhen 518060, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Liu
authors.affiliation: Otolaryngology Institutes, Allergy and Immunology Research Center, Shenzhen University, Shenzhen 518060, China.
authors.forename: Zhilin
authors.initials: Z
authors.lastname: Liang
authors.affiliation: Otolaryngology Institutes, Allergy and Immunology Research Center, Shenzhen University, Shenzhen 518060, China; The State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University; Shenzhen University School of Medicine, 3688 Nanhai Blvd., Shenzhen 518060, China. Electronic address: pcy2356@szu.edu.cn.
authors.forename: Pingchang
authors.initials: P
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Medical College, Guangzhou 510006, China. Electronic address: pxran@vip.163.com.
authors.forename: Pixin
authors.initials: P
authors.lastname: Ran
authors.affiliation: Otolaryngology Institutes, Allergy and Immunology Research Center, Shenzhen University, Shenzhen 518060, China; The State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University; Shenzhen University School of Medicine, 3688 Nanhai Blvd., Shenzhen 518060, China. Electronic address: lzg@szu.edu.cn.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000485:Allergens / Q000235:genetics / Q000276:immunology* / Q000633:toxicity; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000235:genetics / Q000276:immunology* / Q000633:toxicity; D060829:Arthropod Proteins / Q000276:immunology* / Q000633:toxicity; D001249:Asthma / Q000139:chemically induced / Q000276:immunology*; D040001:Dermatophagoides farinae / Q000235:genetics / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D014404:Tubulin / Q000235:genetics / Q000276:immunology* / Q000633:toxicity
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; D014404:Tubulin
keywords: Allergic disease; Dendritic cells; Der f 33; Dermatophagoides farinae