title: Expression of KCNA5 Protein in Human Mammary Epithelial Cell Line Associated with Caveolin-1.
abstract: Voltage-gated potassium (Kv) channels are involved in the proliferation and transformation of mammary epithelial cells. They are thought to be related to the development of breast carcinoma, although the exact role they play in this event remains unclear. In this study, we investigated whether the expression and function of Kv channels is associated with Caveolin-1 (Cav-1, a principal component of caveolae) in different cell lines. We found that expression of Cav-1 correlated with the expression of Kv channels in mammary epithelial cells (MCF10A, MCF10A-ST1, and MCF7), and silencing of Cav-1 inhibited the expression of KCNA5 (voltage-gated shaker-related subfamily A, member 5). Immunofluorescence analysis indicated the colocalization of KCNA5 with Cav-1, whereas immunoprecipitation suggested a possible interaction between the two proteins. Overall, our finding indicated that KCNA5 protein may interact with Cav-1, thereby contributing to the proliferation and early transformation of mammary cells.
authors.affiliation: Department of Biological Science and Technology, Dalian University of Technology, Dalian, 116024, China.|College of Life Science, Liaoning Normal University, No. 1, The South Street of Liushu, Dalian, 116029, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Life Science, Liaoning Normal University, No. 1, The South Street of Liushu, Dalian, 116029, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Qu
authors.affiliation: College of Life Science, Liaoning Normal University, No. 1, The South Street of Liushu, Dalian, 116029, China.|Liaoning Key Laboratories of Biotechnology and Molecular Drug Research and Development, Dalian, 116029, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Life Science, Liaoning Normal University, No. 1, The South Street of Liushu, Dalian, 116029, China.|Liaoning Key Laboratories of Biotechnology and Molecular Drug Research and Development, Dalian, 116029, China.
authors.forename: Yejun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Life Science, Liaoning Normal University, No. 1, The South Street of Liushu, Dalian, 116029, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Sun
authors.affiliation: College of Life Science, Liaoning Normal University, No. 1, The South Street of Liushu, Dalian, 116029, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Yang
authors.affiliation: Centre of Regenerative Medicine, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Biological Science and Technology, Dalian University of Technology, Dalian, 116024, China.
authors.forename: Lijia
authors.initials: L
authors.lastname: An
authors.affiliation: College of Life Science, Liaoning Normal University, No. 1, The South Street of Liushu, Dalian, 116029, China. yuan_zhang84@yahoo.cn.|Liaoning Key Laboratories of Biotechnology and Molecular Drug Research and Development, Dalian, 116029, China. yuan_zhang84@yahoo.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Zou
mesh_terms: D051242:Caveolin 1 / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D015640:Ion Channel Gating; D051666:Kv1.5 Potassium Channel / Q000235:genetics / Q000378:metabolism*; D042361:Mammary Glands, Human / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D021381:Protein Transport
chemical_list: D051242:Caveolin 1; D051666:Kv1.5 Potassium Channel; D024642:Potassium Channels, Voltage-Gated
keywords: Caveolin-1; Human mammary epithelium cell line; KCNA5 protein