title: Tenacibaculum sediminilitoris sp. nov., isolated from a tidal flat.
abstract: A Gram-stain-negative, non-flagellated, non-gliding, non-spore-forming bacterial strain, designated YKTF-3T, was isolated from a tidal flat on the Yellow Sea, South Korea. Strain YKTF-3T grew optimally at 30 °C and in the presence of 2.0-4.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain YKTF-3T fell within the clade comprising the type strains of Tenacibaculumspecies. The novel strain exhibited 16S rRNA gene sequence similarity values of 97.22-98.34 % to the type strains of Tenacibaculum gallaicum, Tenacibaculum ascidiaceicola, Tenacibaculum litoreum, Tenacibaculum discolor, Tenacibaculum aestuarii and Tenacibaculum lutimaris, and of 95.08-96.95 % to the type strains of the other Tenacibaculum species. Strain YKTF-3T contained MK-6 as the predominant menaquinone and iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH as the major fatty acids. The major polar lipids of strain YKTF-3T were phosphatidylethanolamine, one unidentified lipid, one unidentified aminophospholipid, one unidentified glycolipid and one unidentified aminolipid. The DNA G+C content was 32.3 mol% and its mean DNA-DNA relatedness values with the type strains of the six phylogenetically closely relatedTenacibaculum species were 10-27 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain YKTF-3T is separate from other recognized species of the genus Tenacibaculum. On the basis of the data presented, strain YKTF-3T is considered to represent a novel species of the genus Tenacibaculum, for which the name Tenacibaculum sediminilitoris sp. nov. is proposed. The type strain is YKTF-3T (=KCTC 52210T=NBRC 111991T).
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Min-Ji
authors.initials: MJ
authors.lastname: Ha
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.|University of Science and Technology (UST), 113 Gwahak-ro, Yuseong-gu, Daejeon, South Korea.
authors.forename: Yong-Taek
authors.initials: YT
authors.lastname: Jung
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.|University of Science and Technology (UST), 113 Gwahak-ro, Yuseong-gu, Daejeon, South Korea.
authors.forename: Chul-Hyung
authors.initials: CH
authors.lastname: Kang
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D060493:Tenacibaculum / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2