title: [The Comparison Between Extraction Methods of Exosomes From Ascites of Colorectal Cancer].
abstract: OBJECTIVE
  To compare the two different methods to isolate the exosome from the ascites of colorectal cancer (CRC) patient and find the efficient one.
  
  
  METHODS
  Exosome from the ascites of CRC patient were isolated by two different methods: density gradient exosome isolation (DG-Exo) and Exo-Quick isolation, and followed by identification with transmission electron microscopy observation and Western blot analysis. And then, Nanodrop was used for protein quantification.
  
  
  RESULTS
  Exosome were isolated by both of the two methods. The protein concentration of the exosome isolated by the Exo-Quick isolation were higher than that of DG-Exo.
  
  
  CONCLUSIONS
  Exo-Quick isolation can obtain higher purity and more complete exosome from the ascites.
authors.forename: Bing-chen
authors.initials: BC
authors.lastname: Chen
authors.forename: Wan-bin
authors.initials: WB
authors.lastname: He
authors.forename: Xu-yang
authors.initials: XY
authors.lastname: Yang
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.forename: Ting-han
authors.initials: TH
authors.lastname: Yang
authors.forename: Yang-Ping
authors.initials: YP
authors.lastname: Wu
authors.forename: Ming-tian
authors.initials: MT
authors.lastname: Wei
authors.forename: Jin-liang
authors.initials: JL
authors.lastname: Yang
authors.forename: Zi-qiang
authors.initials: ZQ
authors.lastname: Wang
mesh_terms: D001201:Ascites; D015153:Blotting, Western; D015179:Colorectal Neoplasms / Q000473:pathology*; D055354:Exosomes / Q000473:pathology*; D006801:Humans; D046529:Microscopy, Electron, Transmission; D011506:Proteins / Q000302:isolation & purification
chemical_list: D011506:Proteins