title: Interleukin-17A Differentially Induces Inflammatory and Metabolic Gene Expression in the Adipose Tissues of Lean and Obese Mice.
abstract: The functions of interleukin-17A (IL-17A) in adipose tissues and adipocytes have not been well understood. In the present study, male mice were fed with a regular diet (n = 6, lean mice) or a high-fat diet (n = 6, obese mice) for 30 weeks. Subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) were analyzed for IL-17A levels. SAT and VAT were treated with IL-17A and analyzed for inflammatory and metabolic gene expression. Mouse 3T3-L1 pre-adipocytes were differentiated into adipocytes, followed with IL-17A treatment and analysis for inflammatory and metabolic gene expression. We found that IL-17A levels were higher in obese SAT than lean SAT; the basal expression of inflammatory and metabolic genes was different between SAT and VAT and between lean and obese adipose tissues. IL-17A differentially induced expression of inflammatory and metabolic genes, such as tumor necrosis factor α, Il-6, Il-1β, leptin, and glucose transporter 4, in adipose tissues of lean and obese mice. IL-17A also differentially induced expression of inflammatory and metabolic genes in pre-adipocytes and adipocytes, and IL-17A selectively activated signaling pathways in adipose tissues and adipocytes. These findings suggest that IL-17A differentially induces inflammatory and metabolic gene expression in the adipose tissues of lean and obese mice.
authors.affiliation: Department of Structural and Cellular Biology, Tulane University, New Orleans, LA 70112, USA. linlin0852@sina.com.|Department of Histology and Embryology, School of Basic Medicine of North China University of Science and Technology, Tangshan 063000, China. linlin0852@sina.com.
authors.forename: Yine
authors.initials: Y
authors.lastname: Qu
authors.affiliation: Department of Structural and Cellular Biology, Tulane University, New Orleans, LA 70112, USA. qzhang3@tulane.edu.
authors.forename: Qiuyang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Histology and Embryology, School of Basic Medicine of North China University of Science and Technology, Tangshan 063000, China. jckyx@sina.com.
authors.forename: Siqi
authors.initials: S
authors.lastname: Ma
authors.affiliation: Department of Structural and Cellular Biology, Tulane University, New Orleans, LA 70112, USA. sliu1@tulane.edu.
authors.forename: Sen
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Structural and Cellular Biology, Tulane University, New Orleans, LA 70112, USA. czq680302@163.com.|Department of Thoracic Surgery, Affiliated Hospital of North China University of Science and Technology, Tangshan 063000, China. czq680302@163.com.
authors.forename: Zhiquan
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Structural and Cellular Biology, Tulane University, New Orleans, LA 70112, USA. mozhongfu1988@gmail.com.|Department of Obstetrics and Gynecology, Shijiazhuang Maternal and Child Health Care Hospital, Shijiazhuang 050000, China. mozhongfu1988@gmail.com.
authors.forename: Zhongfu
authors.initials: Z
authors.lastname: Mo
authors.affiliation: Department of Structural and Cellular Biology, Tulane University, New Orleans, LA 70112, USA. zyou@tulane.edu.|Department of Orthopaedic Surgery, Tulane University, New Orleans, LA 70112, USA. zyou@tulane.edu.|Tulane Cancer Center and Louisiana Cancer Research Consortium, Tulane University, New Orleans, LA 70112, USA. zyou@tulane.edu.|Tulane Center for Stem Cell Research and Regenerative Medicine, Tulane University, New Orleans, LA 70112, USA. zyou@tulane.edu.|Tulane Center for Aging, Tulane University, New Orleans, LA 70112, USA. zyou@tulane.edu.
authors.forename: Zongbing
authors.initials: Z
authors.lastname: You
mesh_terms: D017667:Adipocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D054392:Adipokines / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D000273:Adipose Tissue / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D002460:Cell Line; D059305:Diet, High-Fat / Q000009:adverse effects; D005786:Gene Expression Regulation; D020381:Interleukin-17 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008820:Mice, Obese; D009765:Obesity / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D015398:Signal Transduction
chemical_list: D054392:Adipokines; D020381:Interleukin-17
keywords: gene expression; inflammation; interleukin-17A; metabolism; obesity