title: Expression and prognostic value of E-cadherin in laryngeal cancer.
abstract: CONCLUSIONS
  Down-regulation of ECD may associate with infiltrative tumor growth and result in poor prognosis. However, ECD expression is not significantly associated with tumor metastasis.
  
  
  OBJECTIVE
  To investigate the prognostic role of E-cadherin (ECD) expression in laryngeal cancer.
  
  
  METHODS
  In a clinical study, a total of 79 laryngeal cancer patients who received surgery were included in this study. The expression of ECD in laryngeal cancer tissue was detected by immumohistochemical staining. In an in vitro study, ECD up-regulated cells (Ad-ECD) and ECD down-regulated cells (sh-ECD) were cultured, along with interference by siRNA. Cell apoptosis was detected by flow cytometry. In an in vivo study, nude mice were injected with Ad-ECD, sh-ECD, and Hep-2 cells. The ECD expression and tumor metastasis in the brain, liver, and lung tissues were detected.
  
  
  RESULTS
  Clinical data showed that expression of ECD was statistically correlated with survival rate (p < 0.05), but not with other clinical characteristics, such as age, gender, lymphatic metastasis, and TNM staging. ECD was down-regulated in sh-ECD cells and the down-regulation significantly reduced apoptosis of Hep-2 cells. Tumor growth was significantly inhibited in the Ad-ECD group. Except the tumor tissue, no positive ECD expression or significant tumor cells were found in the brain, liver, and lung tissues.
authors.affiliation: a Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University , Nanjing , PR China ;|b Department of Otorhinolaryngology-Head and Neck Surgery , Nanjing Drum Tower Hospital Affiliated to Nanjing University Medical School , Nanjing , PR China ;
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Qian
authors.affiliation: b Department of Otorhinolaryngology-Head and Neck Surgery , Nanjing Drum Tower Hospital Affiliated to Nanjing University Medical School , Nanjing , PR China ;
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ma
authors.affiliation: b Department of Otorhinolaryngology-Head and Neck Surgery , Nanjing Drum Tower Hospital Affiliated to Nanjing University Medical School , Nanjing , PR China ;
authors.forename: Han
authors.initials: H
authors.lastname: Zhou
authors.affiliation: b Department of Otorhinolaryngology-Head and Neck Surgery , Nanjing Drum Tower Hospital Affiliated to Nanjing University Medical School , Nanjing , PR China ;
authors.forename: Chenjie
authors.initials: C
authors.lastname: Yu
authors.affiliation: c Department of Pathology , Nanjing Drum Tower Hospital Affiliated to Nanjing University Medical School , Nanjing , PR China.
authors.forename: Yifen
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: b Department of Otorhinolaryngology-Head and Neck Surgery , Nanjing Drum Tower Hospital Affiliated to Nanjing University Medical School , Nanjing , PR China ;
authors.forename: Xia
authors.initials: X
authors.lastname: Yang
authors.affiliation: b Department of Otorhinolaryngology-Head and Neck Surgery , Nanjing Drum Tower Hospital Affiliated to Nanjing University Medical School , Nanjing , PR China ;
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Shen
authors.affiliation: a Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University , Nanjing , PR China ;|b Department of Otorhinolaryngology-Head and Neck Surgery , Nanjing Drum Tower Hospital Affiliated to Nanjing University Medical School , Nanjing , PR China ;
authors.forename: Xia
authors.initials: X
authors.lastname: Gao
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000378:metabolism; D001921:Brain / Q000473:pathology; D015820:Cadherins / Q000378:metabolism*; D002277:Carcinoma / Q000175:diagnosis / Q000378:metabolism*; D045744:Cell Line, Tumor; D005260:Female; D018014:Gene Transfer Techniques; D006801:Humans; D007822:Laryngeal Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D008099:Liver / Q000473:pathology; D008168:Lung / Q000473:pathology; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009374:Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor; D015820:Cadherins
keywords: E-cadherin (ECD); Laryngeal cancer; metastasis; prognosis