title: Virus particle dynamics derived from CryoEM studies.
abstract: The direct electron detector has revolutionized electron cryo-microscopy (CryoEM). Icosahedral virus structures are routinely produced at 4Å resolution or better and the approach has largely displaced virus crystallography, as it requires less material, less purity and often produces a structure more rapidly. Largely ignored in this new era of CryoEM is the dynamic information in the data sets that was not available in X-ray structures. Here we review an approach that captures the dynamic character of viruses displayed in the CryoEM ensemble of particles at the moment of freezing. We illustrate the approach with a simple model, briefly describe the details and provide a practical application to virus particle maturation.
authors.affiliation: Biomedical and Electrical and Computer Engineering, Cornell University, Ithaca, NY, United States.
authors.forename: Peter C
authors.initials: PC
authors.lastname: Doerschuk
authors.affiliation: Electrical and Computer Engineering, Cornell University, Ithaca, NY, United States.
authors.forename: Yunye
authors.initials: Y
authors.lastname: Gong
authors.affiliation: Electrical and Computer Engineering, Cornell University, Ithaca, NY, United States.
authors.forename: Nan
authors.initials: N
authors.lastname: Xu
authors.affiliation: Virology Department, Federal University of Rio de Janeiro Brazil, Rio de Janeiro, Brazil.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Domitrovic
authors.affiliation: Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, United States. Electronic address: jackj@scripps.edu.
authors.forename: John E
authors.initials: JE
authors.lastname: Johnson
mesh_terms: D000465:Algorithms; D000704:Analysis of Variance; D002213:Capsid / Q000502:physiology / Q000648:ultrastructure; D020285:Cryoelectron Microscopy / Q000295:instrumentation / Q000379:methods*; D018360:Crystallography, X-Ray / Q000379:methods; D008958:Models, Molecular; D014771:Virion / Q000502:physiology* / Q000648:ultrastructure*; D019065:Virus Assembly; D014780:Viruses / Q000378:metabolism* / Q000648:ultrastructure*