title: Candidate genes and pathogenesis investigation for sepsis-related acute respiratory distress syndrome based on gene expression profile.
abstract: BACKGROUND
  Acute respiratory distress syndrome (ARDS) is a potentially devastating form of acute inflammatory lung injury as well as a major cause of acute respiratory failure. Although researchers have made significant progresses in elucidating the pathophysiology of this complex syndrome over the years, the absence of a universal detail disease mechanism up until now has led to a series of practical problems for a definitive treatment. This study aimed to predict some genes or pathways associated with sepsis-related ARDS based on a public microarray dataset and to further explore the molecular mechanism of ARDS.
  
  
  RESULTS
  A total of 122 up-regulated DEGs and 91 down-regulated differentially expressed genes (DEGs) were obtained. The up- and down-regulated DEGs were mainly involved in functions like mitotic cell cycle and pathway like cell cycle. Protein-protein interaction network of ARDS analysis revealed 20 hub genes including cyclin B1 (CCNB1), cyclin B2 (CCNB2) and topoisomerase II alpha (TOP2A). A total of seven transcription factors including forkhead box protein M1 (FOXM1) and 30 target genes were revealed in the transcription factor-target gene regulation network. Furthermore, co-cited genes including CCNB2-CCNB1 were revealed in literature mining for the relations ARDS related genes.
  
  
  CONCLUSIONS
  Pathways like mitotic cell cycle were closed related with the development of ARDS. Genes including CCNB1, CCNB2 and TOP2A, as well as transcription factors like FOXM1 might be used as the novel gene therapy targets for sepsis related ARDS.
authors.affiliation: Department of Emergency and Intensive Care Unit, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, 430030, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Emergency and Intensive Care Unit, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, 430030, China.
authors.forename: Jingjun
authors.initials: J
authors.lastname: Yan
authors.affiliation: Institute of Liver Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Xingxing
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Emergency and Intensive Care Unit, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, 430030, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhong
authors.affiliation: Department of Emergency and Intensive Care Unit, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, 430030, China.
authors.forename: Chengye
authors.initials: C
authors.lastname: Zhan
authors.affiliation: Department of Emergency and Intensive Care Unit, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, 430030, China. shushengli063@163.com.
authors.forename: Shusheng
authors.initials: S
authors.lastname: Li
mesh_terms: D002453:Cell Cycle / Q000235:genetics; D030541:Databases, Genetic; D015536:Down-Regulation; D020869:Gene Expression Profiling; D018390:Gene Targeting; D056726:Genetic Association Studies; D006801:Humans; D060066:Protein Interaction Maps; D012128:Respiratory Distress Syndrome / Q000235:genetics*; D018805:Sepsis / Q000150:complications* / Q000235:genetics*; D014157:Transcription Factors; D059467:Transcriptome; D015854:Up-Regulation
chemical_list: D014157:Transcription Factors
keywords: Acute respiratory distress syndrome; Differentially expressed mRNAs; Functional enrichment analysis; Pathway analysis; Sepsis; Transcription factors