title: Photosensitizer-loaded electrospun chitosan-based scaffolds for photodynamic therapy and tissue engineering.
abstract: Novel chitosan-based nanofibrous composite materials containing different amounts of the photosensitizer Photosens were obtained by electrospinning and were characterized by scanning electron microscopy and by confocal laser scanning microscopy. The release of Photosens from the materials was investigated in water and in phosphate-buffered saline. A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line were cultivated on Photosens-containing scaffolds, and cell growth and metabolic activity were examined by confocal laser scanning microscopy and by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay, respectively. The viability of both cell lines on Photosens-containing fibers decreased in a spatial manner upon laser irradiation of an appropriate wavelength and power density. Interestingly, the noncancerous MC3T3-E1 cells grown on Photosens -containing scaffolds were less affected by the irradiation. We conclude that the Photosens-containing electrospun chitosan nanofibers described here are of potential interest for biomedical applications, particularly topical photodynamic therapy and tissue engineering.
authors.affiliation: Institute of Nanostructures and Biosystems, Saratov State University, 410012 Saratov, Russia; Faculty of Nano- and Biomedical Technologies, Saratov State University, 410012 Saratov, Russia. Electronic address: severyuhina_alexandra@mail.ru.
authors.forename: A N
authors.initials: AN
authors.lastname: Severyukhina
authors.affiliation: Institute of Nanostructures and Biosystems, Saratov State University, 410012 Saratov, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Petrova
authors.affiliation: Institute of Transfusion Medicine, Charité-Universitätsmedizin, 10117 Berlin, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Smuda
authors.affiliation: Razumovsky Saratov State Medical University, 410012 Saratov, Russia.
authors.forename: G S
authors.initials: GS
authors.lastname: Terentyuk
authors.affiliation: Institute of Nanostructures and Biosystems, Saratov State University, 410012 Saratov, Russia; Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 410049 Saratov, Russia.
authors.forename: B N
authors.initials: BN
authors.lastname: Klebtsov
authors.affiliation: Institute of Transfusion Medicine, Charité-Universitätsmedizin, 10117 Berlin, Germany; Department of Medical Physics, Biophysics and Radiology, Medical Faculty, Trakia University, 6000 Stara Zagora, Bulgaria.
authors.forename: R
authors.initials: R
authors.lastname: Georgieva
authors.affiliation: Institute of Transfusion Medicine, Charité-Universitätsmedizin, 10117 Berlin, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Bäumler
authors.affiliation: Institute of Nanostructures and Biosystems, Saratov State University, 410012 Saratov, Russia; Faculty of Nano- and Biomedical Technologies, Saratov State University, 410012 Saratov, Russia.
authors.forename: D A
authors.initials: DA
authors.lastname: Gorin
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry*; D006801:Humans; D051379:Mice; D057139:Nanofibers / Q000737:chemistry; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D017319:Photosensitizing Agents; D048271:Chitosan
keywords: Electrospinning; Photoresponsive material; Photosensitizer; Spatially controlled cell proliferation; Tissue-engineering scaffold; Topical photodynamic therapy