title: Clinical applications of MS-based protein quantification.
abstract: Mass spectrometry-based assays are increasingly important in clinical laboratory medicine and nowadays are already commonly used in several areas of routine diagnostics. These include therapeutic drug monitoring, toxicology, endocrinology, pediatrics, and microbiology. Accordingly, some of the most common analyses are therapeutic drug monitoring of immunosuppressants, vitamin D, steroids, newborn screening, and bacterial identification. However, MS-based quantification of peptides and proteins for routine diagnostic use is rather rare up to now despite excellent analytical specificity and good sensitivity. Here, we want to give an overview over current fit-for-purpose assays for MS-based protein quantification. Advantages as well as challenges of this approach will be discussed with focus on feasibility for routine diagnostic use.
authors.affiliation: Institute for Clinical Chemistry, Medical Faculty Mannheim of the University of Heidelberg, University Hospital Mannheim, Mannheim, Germany.
authors.forename: Bassel
authors.initials: B
authors.lastname: Sabbagh
authors.affiliation: Institute for Clinical Chemistry, Medical Faculty Mannheim of the University of Heidelberg, University Hospital Mannheim, Mannheim, Germany.
authors.forename: Sonani
authors.initials: S
authors.lastname: Mindt
authors.affiliation: Institute for Clinical Chemistry, Medical Faculty Mannheim of the University of Heidelberg, University Hospital Mannheim, Mannheim, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Neumaier
authors.affiliation: Institute for Clinical Chemistry, Medical Faculty Mannheim of the University of Heidelberg, University Hospital Mannheim, Mannheim, Germany.|MVZ Labor Dr. Limbach und Kollegen, Heidelberg, Germany.|Working Group Proteomics of the German United Society for Clinical Chemistry and Laboratory Medicine e.V. (DGKL), Bonn, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Findeisen
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D002853:Chromatography, Liquid; D016903:Drug Monitoring / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007166:Immunosuppressive Agents / Q000097:blood; D007231:Infant, Newborn; D013058:Mass Spectrometry; D013677:Medical Laboratory Science / Q000295:instrumentation / Q000379:methods*; D015997:Neonatal Screening; D036361:Peptide Hormones / Q000097:blood; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D011786:Quality Control; D014807:Vitamin D / Q000097:blood
chemical_list: D014408:Biomarkers, Tumor; D001798:Blood Proteins; D007166:Immunosuppressive Agents; D036361:Peptide Hormones; D014807:Vitamin D
keywords: Mass spectrometry; Protein quantification