title: Bidens pilosa and its active compound inhibit adipogenesis and lipid accumulation via down-modulation of the C/EBP and PPARγ pathways.
abstract: Obesity and its complications are a major global health problem. In this study, we investigated the anti-obesity effect and mechanism of an edible plant, Bidens pilosa, and its active constituent. We first assessed the long-term effect of B. pilosa on body composition, body weight, blood parameters in ICR mice. We observed that it significantly decreased fat content and increased protein content in ICR mice. Next, we verified the anti-obesity effect of B. pilosa in ob/ob mice. It effectively and dose-dependently reduced fat content, adipocyte size and/or body weight in mice. Moreover, mechanistic studies showed that B. pilosa inhibited the expression of peroxisome proliferator activated receptor γ (PPARγ), CCAAT/enhancer binding proteins (C/EBPs) and Egr2 in adipose tissue. Finally, we examined the effect of 2-β-D-glucopyranosyloxy-1-hydroxytrideca-5,7,9,11-tetrayne (GHT) on adipogenesis in adipocytes. We found that B. pilosa significantly decreased the adipogenesis and lipid accumulation. This decrease was associated with the down-regulation of expression of Egr2, C/EBPs, PPARγ, adipocyte Protein 2 (aP2) and adiponectin. In summary, this work demonstrated that B. pilosa and GHT suppressed adipogenesis and lipid content in adipocytes and/or animals via the down-regulation of the Egr2, C/EBPs and PPARγ pathways, suggesting a novel application of B. pilosa and GHT against obesity.
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, Taiwan.
authors.forename: Yu-Chuan
authors.initials: YC
authors.lastname: Liang
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, Taiwan.|Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, Taiwan.|Molecular and Biological Agricultural Sciences, Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan, and National Chung-Hsing University, Taichung, Taiwan.
authors.forename: Meng-Ting
authors.initials: MT
authors.lastname: Yang
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, Taiwan.
authors.forename: Chuan-Ju
authors.initials: CJ
authors.lastname: Lin
authors.affiliation: Department of Veterinary Medicine, National Chung-Hsing University, Taiwan.
authors.forename: Cicero Lee-Tian
authors.initials: CL
authors.lastname: Chang
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, Taiwan.|Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, Taiwan.|Molecular and Biological Agricultural Sciences, Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan, and National Chung-Hsing University, Taichung, Taiwan.|Department of Aquaculture, National Taiwan Ocean University, Taiwan.|Institute of Biotechnology, National Taiwan University, Taiwan.
authors.forename: Wen-Chin
authors.initials: WC
authors.lastname: Yang
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000187:drug effects*; D000273:Adipose Tissue / Q000378:metabolism / Q000473:pathology; D000818:Animals; D031176:Bidens / Q000737:chemistry* / Q000378:metabolism; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects*; D051767:Early Growth Response Protein 2 / Q000235:genetics / Q000378:metabolism; D005260:Female; D050356:Lipid Metabolism / Q000187:drug effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008820:Mice, Obese; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D000077154:Rosiglitazone; D015398:Signal Transduction / Q000187:drug effects; D045162:Thiazolidinediones / Q000494:pharmacology
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; D051767:Early Growth Response Protein 2; C497327:Egr2 protein, mouse; D047495:PPAR gamma; D010936:Plant Extracts; D012333:RNA, Messenger; D045162:Thiazolidinediones; D000077154:Rosiglitazone