title: Typing of Plasmodium falciparum DNA from 2 years old Giemsa-stained dried blood spots using nested polymerase chain reaction assay.
abstract: A panel of 129 Giemsa-stained thick blood spots (TBS) confirmed for Plasmodium falciparum infection having different levels of parasite density were collected from a malaria endemic area. DNA was extracted and nested polymerase chain reaction (PCR) assay was performed to amplify P. falciparum DNA. Nested PCR assay successfully amplified P. falciparum DNA at a very low parasitaemia of ~10 parasites/μl of blood. Current PCR assay is very simple and can be used retrospectively to monitor the invasion and prevalence of different Plasmodium species in endemic areas.
authors.forename: D
authors.initials: D
authors.lastname: Kumar
authors.affiliation: Medical Entomology Division, Defence Research Laboratory, Tezpur, Assam, India.
authors.forename: S
authors.initials: S
authors.lastname: Dhiman
authors.forename: B
authors.initials: B
authors.lastname: Rabha
authors.forename: D
authors.initials: D
authors.lastname: Goswami
authors.forename: K
authors.initials: K
authors.lastname: Yadav
authors.forename: M
authors.initials: M
authors.lastname: Deka
authors.forename: V
authors.initials: V
authors.lastname: Veer
authors.forename: I
authors.initials: I
authors.lastname: Baruah
mesh_terms: D000293:Adolescent; D001769:Blood / Q000469:parasitology*; D002648:Child; D016054:DNA, Protozoan / Q000235:genetics*; D004311:Double-Blind Method; D005260:Female; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D016778:Malaria, Falciparum / Q000469:parasitology*; D008297:Male; D010963:Plasmodium falciparum / Q000145:classification* / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012189:Retrospective Studies
chemical_list: D016054:DNA, Protozoan