title: miRNA-154-5p Inhibits Proliferation, Migration and Invasion by Targeting E2F5 in Prostate Cancer Cell Lines.
abstract: BACKGROUND
  MicroRNAs (miRNAs) are a class of small non-coding RNAs (18-25 nucleotides) which post-transcriptionally regulate gene expression by negatively regulating the stability or translational efficiency of their target mRNAs. This study aimed to determine the function of miR-154-5p in prostate cancer (PCa) cells and identify the novel molecular targets regulated by miR-154-5p.
  
  
  METHODS
  The effects of forced miR-154-5p expression or E2F transcription factor 5 (E2F5) knockdown on PCa cells were evaluated by cell proliferation, flow cytometry, cell migration and invasion assays as well as by Western blot analysis. Dual-luciferase reporter assay was performed to verify the precise target of miR-154-5p.
  
  
  RESULTS
  The forced expression of miR-154-5p or E2F5 knockdown significantly restrained cell growth, as well as the migratory and invasive capabilities. Such expression also induced G1 cell cycle arrest of PCa cells in vitro. Hence, E2F5 is a direct target gene of miR-154-5p.
  
  
  CONCLUSIONS
  miR-154-5p may play an important role as an inhibitor of proliferation, migration and invasion of PCa by targeting E2F5 in PCa cell lines.
authors.affiliation: Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zheng
authors.forename: Chen
authors.initials: C
authors.lastname: Zhu
authors.forename: Long
authors.initials: L
authors.lastname: Ma
authors.forename: Pengfei
authors.initials: P
authors.lastname: Shao
authors.forename: Chao
authors.initials: C
authors.lastname: Qin
authors.forename: Pu
authors.initials: P
authors.lastname: Li
authors.forename: Qiang
authors.initials: Q
authors.lastname: Cao
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Ju
authors.forename: Gong
authors.initials: G
authors.lastname: Cheng
authors.forename: Qingyi
authors.initials: Q
authors.lastname: Zhu
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Gu
authors.forename: Lixin
authors.initials: L
authors.lastname: Hua
mesh_terms: D000368:Aged; D002465:Cell Movement; D049109:Cell Proliferation; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000502:physiology*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D011471:Prostatic Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: C561122:MIRN154 microRNA, human; D035683:MicroRNAs