title: [Content determination of dencichine in Panax Notoginseng by a reversed phase ion-pair chromatography].
abstract: To build a reversed phase ion-pair chromatography to determination content of Dencichine from Panax notoginseng. Using Tetrabutyl ammonium hydroxide ions by the combination of reagent and HPLC method without derivatization to test the content of dencichine directly. The optimum conditions of supersonic extraction were solid-to-liquid ratio 1: 20, Continuous ultrasonic extraction: twice, each time 15 minutes; 3,500 r · min⁻¹, then centrifuging 15 minutes. Dencichine in different age, place, part and the different Processing mode were examined. The method is simple with sound separation degree and stability, which can facilitate the determination of dencichine content directly and provide the basis in quality standard of raw material.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Cheng-xiao
authors.initials: CX
authors.lastname: Wang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Qu
authors.forename: Xiu-ming
authors.initials: XM
authors.lastname: Cui
mesh_terms: D000599:Amino Acids, Diamino / Q000032:analysis*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D053723:Panax notoginseng / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry
chemical_list: D000599:Amino Acids, Diamino; D004365:Drugs, Chinese Herbal; C100126:oxalyldiaminopropionic acid