title: Development of House Dust Mite Vaccine.
abstract: Mucosal vaccine based on lactic acid bacteria is an attractive strategy for prevention and treatment of allergic diseases. Here we describe the development of recombinant Lactococcus lactis expressing house dust mite (HDM) allergen as an oral vaccine. The major HDM allergen Der p2 is first codon optimized and synthesized to achieve the maximum expression level in L. lactis. After double digested by NcoI and XbaI, the derp2 fragment is ligated to the same double-digested pNZ8148 vector. The ligation is transformed to L. lactis NZ9000 and correct transformant is verified by sequencing. Western blot analysis is employed to confirm Derp2 expression in L. lactis after nisin induction.
authors.affiliation: School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, Jiangsu, People's Republic of China. zhangqx@jiangnan.edu.cn.
authors.forename: Qiuxiang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, Jiangsu, People's Republic of China.
authors.forename: Chunqing
authors.initials: C
authors.lastname: Ai
mesh_terms: D000818:Animals; D039741:Antigens, Dermatophagoides / Q000737:chemistry / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005818:Genetic Engineering / Q000379:methods*; D013294:Lactococcus lactis / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D039981:Pyroglyphidae / Q000276:immunology*; D014170:Transformation, Genetic; D014612:Vaccines / Q000737:chemistry / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D039741:Antigens, Dermatophagoides; C559662:Derp2 allergen, Dermatophagoides pteronyssinus; D014612:Vaccines
keywords: Allergy; House dust mite; Lactic acid bacteria; Mucosal vaccine