title: Simultaneous detection of Waddlia chondrophila and Listeria monocytogenes in aborted ruminant samples by real-time quantitative PCR.
authors.affiliation: Toxicology-Microbiology and Environmental Health Unit (UR11ES70), Faculty of Sciences, University of Sfax, Tunisia. Electronic address: mohamedbarkallah@gmail.com.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Barkallah
authors.affiliation: Toxicology-Microbiology and Environmental Health Unit (UR11ES70), Faculty of Sciences, University of Sfax, Tunisia.
authors.forename: Yaakoub
authors.initials: Y
authors.lastname: Gharbi
authors.affiliation: Toxicology-Microbiology and Environmental Health Unit (UR11ES70), Faculty of Sciences, University of Sfax, Tunisia.
authors.forename: Ahlem Ben
authors.initials: AB
authors.lastname: Slima
authors.affiliation: Toxicology-Microbiology and Environmental Health Unit (UR11ES70), Faculty of Sciences, University of Sfax, Tunisia.
authors.forename: Fatma
authors.initials: F
authors.lastname: Elleuch
authors.affiliation: Veterinary Research Center of Sfax, Sfax, Tunisia.
authors.forename: Zouhir
authors.initials: Z
authors.lastname: Mallek
authors.affiliation: Centre of Biotechnology of Sfax (CBS), LPAP, University of Sfax, Tunisia.
authors.forename: Rania Ben
authors.initials: RB
authors.lastname: Saad
authors.affiliation: Equipe Microbiologie de l'œuf et des Ovoproduits (MICOV), Agrocampus Ouest, INRA, (UMR1253) Science et Technologie du Lait et de l'Œuf, Rennes, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Gautier
authors.affiliation: Toxicology-Microbiology and Environmental Health Unit (UR11ES70), Faculty of Sciences, University of Sfax, Tunisia.
authors.forename: Radhouane
authors.initials: R
authors.lastname: Gdoura
authors.affiliation: Toxicology-Microbiology and Environmental Health Unit (UR11ES70), Faculty of Sciences, University of Sfax, Tunisia.
authors.forename: Imen
authors.initials: I
authors.lastname: Fendri
mesh_terms: D000031:Abortion, Septic / Q000175:diagnosis / Q000662:veterinary*; D000818:Animals; D002695:Chlamydiales / Q000302:isolation & purification*; D005260:Female; D008089:Listeria monocytogenes / Q000302:isolation & purification*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D011247:Pregnancy; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012418:Ruminants; D012680:Sensitivity and Specificity
keywords: Absolute quantification; Biological samples; Co-culture; Intracellular bacteria; Recombinant plasmids; qPCR