title: Effective pretreatment of sugarcane bagasse with combination pretreatment and its hydrolyzates as reaction media for the biosynthesis of ethyl (S)-4-chloro-3-hydroxybutanoate by whole cells of E. coli CCZU-K14.
abstract: In this study, sugarcane bagasse (SB) was pretreated with combination pretreatment (e.g., sequential KOH extraction and ionic liquid soaking, sequential KOH extraction and Fenton soaking, or sequential KOH extraction and glycerol soaking). After the enzymatic hydrolysis of pretreated SBs, it was found that all these three concentrated hydrolyzates could be used for the asymmetric bioreduction of ethyl 4-chloro-3-oxobutanoate (COBE) into ethyl (S)-4-chloro-3-hydroxybutanoate [(S)-CHBE]. Compared with glucose, arabinose and cellobiose couldn't promote the initial reaction rate, and xylose could increase the intracellular NADH content. Moreover, it was the first report that hydrolyzates could be used for the effective biosynthesis of (S)-CHBE (∼500g/L; 98.0% yield) from 3000 COBE by whole cells of Escherichia coli CCZU-K14 in the presence of β-CD (0.4mol β-CD/mol COBE), l-glutamine (200mM) and glycine (500mM). In conclusion, it is a new alternative to utilize bioresource for the synthesis of key chiral intermediate (S)-CHBE.
authors.affiliation: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China; Bioproducts, Sciences and Engineering Laboratory, Department of Biological Systems Engineering, Washington State University, Richland, WA 99354, USA. Electronic address: heyucai2001@163.com.
authors.forename: Yu-Cai
authors.initials: YC
authors.lastname: He
authors.affiliation: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Dan-Ping
authors.initials: DP
authors.lastname: Zhang
authors.affiliation: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Jun-Hua
authors.initials: JH
authors.lastname: Di
authors.affiliation: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Yin-Qi
authors.initials: YQ
authors.lastname: Wu
authors.affiliation: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Zhi-Cheng
authors.initials: ZC
authors.lastname: Tao
authors.affiliation: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Zhang
authors.affiliation: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Gang-Gang
authors.initials: GG
authors.lastname: Chong
authors.affiliation: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Cui-Luan
authors.initials: CL
authors.lastname: Ma
mesh_terms: D001711:Biotransformation / Q000187:drug effects; D002087:Butyrates / Q000378:metabolism*; D002241:Carbohydrates / Q000032:analysis; D002482:Cellulose / Q000737:chemistry*; D004926:Escherichia coli / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D005973:Glutamine / Q000494:pharmacology; D005998:Glycine / Q000494:pharmacology; D006868:Hydrolysis; D031786:Saccharum / Q000737:chemistry*; D012995:Solubility; D013997:Time Factors; D047392:beta-Cyclodextrins / Q000494:pharmacology
chemical_list: D002087:Butyrates; D002241:Carbohydrates; D047392:beta-Cyclodextrins; C121244:ethyl 4-chloro-3-hydroxybutanoate; D005973:Glutamine; D002482:Cellulose; C027433:bagasse; D005998:Glycine
keywords: Asymmetric bioreduction; Combination pretreatment; Ethyl (S)-4-chloro-3-hydroxybutanoate; Ethyl 4-chloro-3-oxobutanoate; Sugarcane bagasse