title: Pestivirus in alpine wild ruminants and sympatric livestock from the Cantabrian Mountains, Spain.
abstract: Bovine viral diarrhoea virus (BVDV) and Border disease virus (BDV) were investigated at the wildlife-livestock interface in the distribution area of chamois in the Cantabrian Mountains, North-Western Spain. From 2010 to 2014, sera from sympatric wild (n=167) and domestic (n=272) ruminants were analysed for pestivirus antibodies by cELISA, virus neutralisation test (VNT) and for the presence of pestiviral RNA using a reverse transcription (RT)-PCR. Results showed a higher seroprevalence in cattle (59.4 per cent, 13/13 of herds) than in domestic small ruminants (5.9 per cent sheep, 2/8 of flocks; 0 per cent goats of 4 flocks) and wildlife (10.8 per cent in red deer, 0 per cent in roe deer and 0 per cent in Cantabrian chamois). High VNT titres were detected in two cattle herds, suggesting the circulation of BVDV-1 and BVDV-2 strains. BVDV-1 RNA was detected in one cattle calf by RT-PCR and sequencing. Conversely to other similar grazing systems, sheep flocks did not play a relevant role in the pestivirus epidemiology in this region. Pestivirus infections in wild ruminants were sporadic and most probably dependent on a domestic source.
authors.affiliation: Servei d'Ecopatologia de Fauna Salvatge, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra 08193, Spain.
authors.forename: X
authors.initials: X
authors.lastname: Fernández-Aguilar
authors.affiliation: Servei d'Ecopatologia de Fauna Salvatge, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra 08193, Spain.
authors.forename: J R
authors.initials: JR
authors.lastname: López-Olvera
authors.affiliation: Servei d'Ecopatologia de Fauna Salvatge, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra 08193, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Marco
authors.affiliation: IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, Bellaterra 08193, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Rosell
authors.affiliation: Servei d'Ecopatologia de Fauna Salvatge, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra 08193, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Colom-Cadena
authors.affiliation: Servei d'Ecopatologia de Fauna Salvatge, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra 08193, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Soto-Heras
authors.affiliation: Servei d'Ecopatologia de Fauna Salvatge, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra 08193, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Lavín
authors.affiliation: Servei d'Ecopatologia de Fauna Salvatge, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra 08193, Spain.
authors.forename: O
authors.initials: O
authors.lastname: Cabezón
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000821:virology*; D058751:Livestock / Q000821:virology*; D010576:Pestivirus / Q000302:isolation & purification*; D018182:Pestivirus Infections / Q000453:epidemiology / Q000662:veterinary*; D012418:Ruminants / Q000821:virology*; D013030:Spain / Q000453:epidemiology
keywords: Border disease; Bovine viral diarrhoea virus (BVDV); Chamois; Livestock; reed deer; roe deer