title: Utilization of recovered nitrogen from hydrothermal carbonization process by Arthrospira platensis.
abstract: In the context of sustainable cultivation of microalgae, the present study focused on the use of nitrogen from the hot-water extracted biomass residue of Arthrospira platensis by hydrothermal carbonization (HTC) and the sequential cultivation of the same alga with the HTC aqueous phase (AP). Nearly 90% of the nitrogen recovered from HTC into AP was in the organic form. Under nitrogen-limited condition with HTCAP as nitrogen source the yield and content of carbohydrate were enhanced by 21% and 15% respectively compared with that under the same nitrogen level provided by NaNO3, which entitled HTCAP for the substitution of conventional nitrate. In the same way pilot-scale cultivation of A. platensis in raceway ponds outdoors demonstrated that carbohydrate content of 43.8% DW and productivity of 10.3g/m(2)/d was achieved. Notably 54% of organic nitrogen in the HTCAP could be recycled by cultivation of pre-nitrogen starved A. platensis as seeds under nitrogen limitation.
authors.affiliation: Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China.
authors.forename: Changhong
authors.initials: C
authors.lastname: Yao
authors.affiliation: Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China; College of Environment and Chemical Engineering, Dalian University, Dalian 116622, PR China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Lu
authors.affiliation: Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China.
authors.forename: Peichun
authors.initials: P
authors.lastname: Wu
authors.affiliation: Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China; School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, PR China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Meng
authors.affiliation: Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China.
authors.forename: Xupeng
authors.initials: X
authors.lastname: Cao
authors.affiliation: Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China. Electronic address: xuesong@dicp.ac.cn.
authors.forename: Song
authors.initials: S
authors.lastname: Xue
mesh_terms: D018533:Biomass; D002244:Carbon / Q000378:metabolism; D058086:Microalgae / Q000254:growth & development / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism; D009584:Nitrogen / Q000737:chemistry / Q000378:metabolism*; D053545:Spirulina / Q000254:growth & development / Q000378:metabolism*
chemical_list: D009566:Nitrates; D002244:Carbon; D009584:Nitrogen
keywords: Arthrospira platensis; Carbohydrate; Hydrothermal carbonization; Microalgae cultivation; Nitrogen limitation; Nitrogen recycling