title: Biased expression, under the control of single promoter, of human interferon α-2b and Escherichia coli methionine amino peptidase genes in E. coli, irrespective of their distance from the promoter.
abstract: Human interferon α-2b and Escherichia coli methionine amino peptidase genes were cloned independently as well as bicistronically in expression plasmid pET-21a (+). Production of human interferon α-2b was comparable to that of E. coli methionine amino peptidase when these genes were expressed independently in E. coli BL21-CodonPlus (DE3)-RIL. However, human interferon α-2b was produced in a much less amount whereas there was no difference in the production of methionine amino peptidase when the encoding genes were expressed bicistronically. It is important to note that human interferon α-2b was the first gene in order, after the promoter and E. coli methionine amino peptidase was the next with a linker sequence of 27 nucleotides between them.
authors.affiliation: School of Biological Sciences, University of the Punjab, Quaid-e-Azam Campus, Lahore, Pakistan.
authors.forename: Amina
authors.initials: A
authors.lastname: Arif
authors.affiliation: School of Biological Sciences, University of the Punjab, Quaid-e-Azam Campus, Lahore, Pakistan.
authors.forename: Naeem
authors.initials: N
authors.lastname: Rashid
authors.affiliation: Department of Biotechnology, Lahore College for Women University, Jail Road, Lahore, Pakistan.
authors.forename: Farheen
authors.initials: F
authors.lastname: Aslam
authors.affiliation: Department of Allied Health Sciences and Chemical Pathology, University of Health Sciences, Lahore, Pakistan.
authors.forename: Nasir
authors.initials: N
authors.lastname: Mahmood
authors.affiliation: School of Biological Sciences, University of the Punjab, Quaid-e-Azam Campus, Lahore, Pakistan / School of Biological Sciences, University of Southampton, Southampton SO16 7PX, UK.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Akhtar
mesh_terms: D000626:Aminopeptidases / Q000096:biosynthesis* / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000096:biosynthesis* / Q000235:genetics; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection
chemical_list: D029968:Escherichia coli Proteins; C554158:IFNA2 protein, human; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins; D000626:Aminopeptidases