title: Development of microsatellite markers for Manilkara maxima T.D. Penn. (Sapotaceae) and their use in conservation genetics.
abstract: Manilkara maxima is an endemic tree species of the Atlantic Forest in southern Bahia, Brazil. It is considered important for forest conservation due to its mutualistic interactions with endemic and endangered animals. Our aim was to develop microsatellite markers to estimate genetic diversity in order to provide information for effectiveness of future conservation programs. We used next generation sequencing technology to develop the first specific microsatellite markers for M. maxima. Seventeen new microsatellite loci were applied in 72 individuals sampled in three natural populations. On average, the number of alleles per loci was 8.8. The expected heterozygosity varied between 0.72 and 0.77, indicating that the developed set of molecular markers is useful for genetic diversity studies. Additionally, the estimated value for the combined probability of exclusion (Q) was greater than 0.999, which indicates the powerful of these molecular tools for paternity and kinship analysis. Our results demonstrate that the set of microsatellites developed in this work is a powerful tool for population genetics, molecular ecology and conservation biology purposes.
authors.affiliation: Pós-graduação em Genética e Biologia Molecular, Universidade Estadual de Santa Cruz (UESC), Rodovia Ilhéus-Itabuna km 16 s/n, Salobrinho, Ilhéus, Bahia, 45662-900, Brazil.
authors.forename: José Audenor
authors.initials: JA
authors.lastname: Silva-Junior
authors.affiliation: Pós-graduação em Genética e Biologia Molecular, Universidade Estadual de Santa Cruz (UESC), Rodovia Ilhéus-Itabuna km 16 s/n, Salobrinho, Ilhéus, Bahia, 45662-900, Brazil.
authors.forename: Daniele
authors.initials: D
authors.lastname: de Souza França
authors.affiliation: Pós-graduação em Ecologia e Conservação da Biodiversidade, Universidade Estadual de Santa Cruz (UESC), Rodovia Ilhéus-Itabuna km 16 s/n, Salobrinho, Ilhéus, Bahia, 45662-900, Brazil.
authors.forename: Ramiris César Souza
authors.initials: RC
authors.lastname: Moraes
authors.affiliation: Pós-graduação em Genética e Biologia Molecular, Universidade Estadual de Santa Cruz (UESC), Rodovia Ilhéus-Itabuna km 16 s/n, Salobrinho, Ilhéus, Bahia, 45662-900, Brazil. gaiotto@uesc.br.|Departamento de Ciências Biológicas, Laboratório de Marcadores Moleculares, Centro de Biotecnologia e Genética, Universidade Estadual de Santa Cruz (UESC), Rodovia Ilhéus-Itabuna km 16 s/n, Salobrinho, Ilhéus, Bahia, 45662-900, Brazil. gaiotto@uesc.br.
authors.forename: Fernanda Amato
authors.initials: FA
authors.lastname: Gaiotto
mesh_terms: D003247:Conservation of Natural Resources; D017343:Genes, Plant; D005819:Genetic Markers; D014644:Genetic Variation; D005838:Genotype; D059014:High-Throughput Nucleotide Sequencing; D032129:Manilkara / Q000235:genetics*; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D005819:Genetic Markers
keywords: Conservation; Molecular markers; NGS; Population genetics; SSR; Tropical forest