title: Toxicity assessment of four insecticides with different modes of action on pupae and adults of Eriopis connexa (Coleoptera: Coccinellidae), a relevant predator of the Neotropical Region.
abstract: Pesticides can be toxic to nontarget organisms including the natural enemies of agricultural pests, thus reducing the biodiversity of agroecosystems. The lethal and sublethal effects of four insecticides with different modes of action-pyriproxyfen, teflubenzuron, acetamiprid, and cypermethrin-were evaluated on pupae and adults of Eriopis connexa, an effective predator in horticultural crops. Pupal survival was reduced by pyriproxyfen (26 %) and cypermethrin (41 %). Malformations in adults emerged from treated pupae were observed after acetamiprid (82.7 and 100 % for 100 and 200 mg a.i./l, respectively), pyriproxyfen (48.6 %), and cypermethrin (13.3 %) treatments. A longer mean oviposition time was also observed in adults emerged from pupae treated with cypermethrin. Moreover, the latter insecticide as well as teflubenzuron did not reduce reproductive parameters, whereas females emerged from pyriproxyfen-treated pupae were not be able to lay eggs even when females showed large abdomens. Upon exposure of adults, survival was reduced to approximately 90 % by acetamiprid, but no reduction occurred with pyriproxyfen, teflubenzuron, or cypermethrin though the fecundity at fifth oviposition time of the female survivors was reduced. Pyriproxyfen decreased the hatching at all the oviposition times tested, whereas fertility was reduced in the fourth and fifth ovipositions by teflubenzuron and in the first and third ovipositions by cypermethrin. In conclusion, all four insecticides tested exhibited lethal or sublethal effects, or both, on E. connexa. The neurotoxic insecticides were more harmful than the insect-growth regulators, and pupae were more susceptible than adults. The toxicity of insecticides on the conservation of predators in agroecosystems of the Neotropical Region is discussed.
authors.affiliation: Laboratorio de Ecotoxicología: Plaguicidas y Control Biológico, Centro de Estudios Parasitológicos y de Vectores (CEPAVE-CONICET, Universidad Nacional de La Plata), Boulevard 120 s/n entre 61 y 64, 1900, La Plata, Buenos Aires, Argentina.|Centro de Investigaciones del Medio Ambiente (CIMA), Facultad de Ciencias Exactas, CONICET, Universidad Nacional de La Plata, Calle 47 y 115, 1900, La Plata, Buenos Aires, Argentina.
authors.forename: Marilina Noelia
authors.initials: MN
authors.lastname: Fogel
authors.affiliation: Laboratorio de Ecotoxicología: Plaguicidas y Control Biológico, Centro de Estudios Parasitológicos y de Vectores (CEPAVE-CONICET, Universidad Nacional de La Plata), Boulevard 120 s/n entre 61 y 64, 1900, La Plata, Buenos Aires, Argentina. mschneider@cepave.edu.ar.|Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 60 y 122 s/n, 1900, La Plata, Buenos Aires, Argentina. mschneider@cepave.edu.ar.
authors.forename: Marcela Inés
authors.initials: MI
authors.lastname: Schneider
authors.affiliation: Centro de Investigaciones del Medio Ambiente (CIMA), Facultad de Ciencias Exactas, CONICET, Universidad Nacional de La Plata, Calle 47 y 115, 1900, La Plata, Buenos Aires, Argentina.
authors.forename: Federico
authors.initials: F
authors.lastname: Rimoldi
authors.affiliation: Laboratorio de Ecotoxicología: Plaguicidas y Control Biológico, Centro de Estudios Parasitológicos y de Vectores (CEPAVE-CONICET, Universidad Nacional de La Plata), Boulevard 120 s/n entre 61 y 64, 1900, La Plata, Buenos Aires, Argentina.
authors.forename: Lorena Sabrina
authors.initials: LS
authors.lastname: Ladux
authors.affiliation: French National Institute for Agricultural Research (INRA), UMR-ISA, 400 Route des Chappes, 06903, Sophia-Antipolis, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Desneux
authors.affiliation: Centro de Investigaciones del Medio Ambiente (CIMA), Facultad de Ciencias Exactas, CONICET, Universidad Nacional de La Plata, Calle 47 y 115, 1900, La Plata, Buenos Aires, Argentina.
authors.forename: Alicia Estela
authors.initials: AE
authors.lastname: Ronco
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000187:drug effects*; D005260:Female; D007306:Insecticides / Q000633:toxicity*; D010058:Oviposition / Q000187:drug effects; D011679:Pupa / Q000187:drug effects*; D014329:Tropical Climate
chemical_list: D007306:Insecticides
keywords: Eriopis connexa; Insecticides selectivity; Lethal effects; Sublethal effects