title: DNA damage responses in mammalian oocytes.
abstract: DNA damage acquired during meiosis can lead to infertility and miscarriage. Hence, it should be important for an oocyte to be able to detect and respond to such events in order to make a healthy egg. Here, the strategies taken by oocytes during their stages of growth to respond to DNA damaging events are reviewed. In particular, recent evidence of a novel pathway in fully grown oocytes helps prevent the formation of mature eggs with DNA damage. It has been found that fully grown germinal vesicle stage oocytes that have been DNA damaged do not arrest at this point in meiosis, but instead undergo meiotic resumption and stall during the first meiotic division. The Spindle Assembly Checkpoint, which is a well-known mitotic pathway employed by somatic cells to monitor chromosome attachment to spindle microtubules, appears to be utilised by oocytes also to respond to DNA damage. As such maturing oocytes are arrested at metaphase I due to an active Spindle Assembly Checkpoint. This is surprising given this checkpoint has been previously studied in oocytes and considered to be weak and ineffectual because of its poor ability to be activated in response to microtubule attachment errors. Therefore, the involvement of the Spindle Assembly Checkpoint in DNA damage responses of mature oocytes during meiosis I uncovers a novel second function for this ubiquitous cellular checkpoint.
authors.affiliation: Centre for Biological SciencesFaculty of Natural and Environmental Sciences, University of Southampton, Southampton, UK k.t.jones@soton.ac.uk; jkc1g10@soton.ac.uk.
authors.forename: Josie K
authors.initials: JK
authors.lastname: Collins
authors.affiliation: Centre for Biological SciencesFaculty of Natural and Environmental Sciences, University of Southampton, Southampton, UK k.t.jones@soton.ac.uk; jkc1g10@soton.ac.uk.
authors.forename: Keith T
authors.initials: KT
authors.lastname: Jones
mesh_terms: D000818:Animals; D059447:Cell Cycle Checkpoints; D004249:DNA Damage; D005260:Female; D006801:Humans; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D008941:Spindle Apparatus / Q000502:physiology*