title: Adenosine A1 receptor modifies P53 expression and apoptosis in breast cancer cell line Mcf-7.
abstract: BACKGROUND
  Breast cancer cells over-express the adenosine receptor A1 and in most of these cells, P53 gene is a wild type. Because of this finding and relationship between A1 receptor and cell apoptosis and proliferation, this study aimed to determine the effect of agonist and antagonist of A1 receptor on cell apoptosis and proliferation and recognize the relationship between this receptor and P53 expression.
  
  
  METHODS
  We used a Real-Time PCR test for measuring expression of p53 gene also flow cytometry assay for apoptotic and survival cell rate after treatment of MCF-7 cells with A1 receptor agonist CPA (N6-Cyclopentyladenosine) and A1 receptor antagonist DPCPX (1,3-dipropyl-8-cyclopentylxanthine) in 24,48 and 72 hours.
  
  
  RESULTS
  Our flow cytometry findings indicate that DPCPX significantly induces apoptosis in MCF-7. Also the expression of P53 becomes upregulated with time of DPCPX treatment. CPA treatment increased the survival cell rate and down-regulated this apoptosis-relevant gene P53 (p > 0.05).
  
  
  CONCLUSIONS
  DPCPX can induce P53 expression which consequently promotes the cell apoptosis in MCF-7. Therefore, DPCPX could be used as an anti-cancer agent (Tab. 1, Fig. 3, Ref. 5).
authors.forename: M Nikbakht
authors.initials: MN
authors.lastname: Dastjerdi
authors.forename: A
authors.initials: A
authors.lastname: Valiani
authors.forename: M
authors.initials: M
authors.lastname: Mardani
authors.forename: M Zamani
authors.initials: MZ
authors.lastname: Ra
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D058907:Adenosine A1 Receptor Agonists / Q000494:pharmacology; D058916:Adenosine A1 Receptor Antagonists / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D020869:Gene Expression Profiling; D016158:Genes, p53 / Q000235:genetics; D006801:Humans; D061986:MCF-7 Cells; D043682:Receptor, Adenosine A1 / Q000378:metabolism*; D014970:Xanthines / Q000494:pharmacology*
chemical_list: D058907:Adenosine A1 Receptor Agonists; D058916:Adenosine A1 Receptor Antagonists; D043682:Receptor, Adenosine A1; D014970:Xanthines; C048599:N(6)-cyclopentyladenosine; C051360:1,3-dipropyl-8-cyclopentylxanthine; D000241:Adenosine
keywords: MCF-7 cells.; adenosine A1; apoptosis; gene; p53; receptor