title: Comparison Between an Automated and Manual Extraction for the Determination of Immunosuppressive Drugs Whole Blood Concentrations by Liquid Chromatography Tandem Mass Spectrometry.
abstract: BACKGROUND
  The whole blood extraction for liquid chromatography tandem mass spectrometry (LC-MS/MS) of simultaneous quantification of cyclosporine A (Cys A), tacrolimus (Tacrs), sirolimus (Siros), and everolimus (Evers) is still performed manually in many laboratories. The analytical results obtained with an automated method using a liquid handler versus a classical manual preparation were compared.
  
  
  METHODS
  Cys A (n = 36), Tacrs (n = 50), Siros (n = 34), Evers (n = 38) whole blood samples of patients were analyzed by LC-MS/MS assay after manual preparation and automated process using a liquid handling platform including a centrifugation step.
  
  
  RESULTS
  The comparison between manual and automated extraction investigated by a linear regression showed a high correlation between results [(Tacrs "automated") = 1.0927 × (Tacrs "manual") - 0.36; (Cys A "automated") = 1.0284 × (Cys A "manual") + 0.0312; (Siros "automated") = 0.9923 × (Siros "manual") + 0.4001; (Evers "automated") = 1.0000 × (Evers "manual") - 0.0600].
  
  
  CONCLUSIONS
  The results obtained by the automated and manual preparation are consistent. The automated method is applied for high-throughput therapeutic drug monitoring of immunosuppressive drugs in routine practice, leading to an increase in quality.
authors.affiliation: Clinical Pharmacology Department, University Hospital of Nantes, Nantes, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Deslandes
authors.affiliation: Clinical Pharmacology Department, University Hospital of Nantes, Nantes, France.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Grégoire
authors.affiliation: Clinical Pharmacology Department, University Hospital of Nantes, Nantes, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Renaud
authors.affiliation: Clinical Pharmacology Department, University Hospital of Nantes, Nantes, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Monteil-Ganière
authors.affiliation: Clinical Pharmacology Department, University Hospital of Nantes, Nantes, France.
authors.forename: Claudine
authors.initials: C
authors.lastname: Azoulay
authors.affiliation: Clinical Pharmacology Department, University Hospital of Nantes, Nantes, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Pineau
authors.affiliation: Clinical Pharmacology Department, University Hospital of Nantes, Nantes, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Jolliet
authors.affiliation: Clinical Pharmacology Department, University Hospital of Nantes, Nantes, France. eric.dailly@chu-nantes.fr.
authors.forename: Eric
authors.initials: E
authors.lastname: Dailly
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D016903:Drug Monitoring / Q000379:methods*; D001330:Electronic Data Processing / Q000379:methods*; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000097:blood*; D016014:Linear Models; D008297:Male; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D007166:Immunosuppressive Agents
keywords: automation; cyclosporine A; everolimus; sirolimus; tacrolimus