title: [HPLC simultaneous determination of contents of 5 saponin constituents in Ophiopogonis Radix].
abstract: This research is to establish an HPLC method for the simultaneous determination of ophiopogonin D, ophiopogonin D', ophiopogonin C, deacetylophiopojaponin A and ophiogenin-3-O-α-L-rhamnosyl-(1-->2)-β-D-glucoside in Ophiopogonis Radix. HPLC-ELSD analysis was performed on a Kromasil 100-5 C₁₈ column (4.6 mm x 250 mm, 5 µm), with the mobile phase of acetonitrile (A) -water (B) in gradient elution mode (0-45 min, 35%-55% A), at a flow rate of 1 mL · min⁻¹. The column temperature was 35 °C and the drift tube temperature was 100 °C in a gas flow rate of 3.0 L · min⁻¹. The result showed that baseline of all the 5 constituents was well separated, and every constituent had wide linearity range and good linear relation (r > 0.999). The recovery rate was between 95.75% and 103.1%. The new established method for simultaneous determination of saponin constituents in Ophiopogonis Radix was sensitive and has good, repeatability. It could be applied to quality evaluation of Ophiopogonis Radix.
authors.forename: Fa-ming
authors.initials: FM
authors.lastname: Wu
authors.forename: Xiao-yang
authors.initials: XY
authors.lastname: Cai
authors.forename: Pan
authors.initials: P
authors.lastname: Wang
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Bao
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Juan
authors.initials: J
authors.lastname: Zhou
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D031441:Ophiopogon / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D004365:Drugs, Chinese Herbal; D012503:Saponins