title: Metabolomics reveals mycoplasma contamination interferes with the metabolism of PANC-1 cells.
abstract: Mycoplasma contamination is a common problem in cell culture and can alter cellular functions. Since cell metabolism is either directly or indirectly involved in every aspect of cell function, it is important to detect changes to the cellular metabolome after mycoplasma infection. In this study, liquid chromatography mass spectrometry (LC/MS)-based metabolomics was used to investigate the effect of mycoplasma contamination on the cellular metabolism of human pancreatic carcinoma cells (PANC-1). Multivariate analysis demonstrated that mycoplasma contamination induced significant metabolic changes in PANC-1 cells. Twenty-three metabolites were identified and found to be involved in arginine and purine metabolism and energy supply. This study demonstrates that mycoplasma contamination significantly alters cellular metabolite levels, confirming the compelling need for routine checking of cell cultures for mycoplasma contamination, particularly when used for metabolomics studies. Graphical abstract Metabolomics reveals mycoplasma contamination changes the metabolome of PANC-1 cells.
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-sen University, 132# Waihuandong Rd, University City of Guangzhou, Guangzhou, 510006, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yu
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-sen University, 132# Waihuandong Rd, University City of Guangzhou, Guangzhou, 510006, China.
authors.forename: Yongtao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-sen University, 132# Waihuandong Rd, University City of Guangzhou, Guangzhou, 510006, China.
authors.forename: Huizhen
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Environmental Health Sciences, Yale School of Public Health, Yale University, 60 College Street, New Haven, CT, 06520, USA.
authors.forename: Caroline H
authors.initials: CH
authors.lastname: Johnson
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-sen University, 132# Waihuandong Rd, University City of Guangzhou, Guangzhou, 510006, China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Thermo Fisher Scientific, Xin Jinqiao Rd., Shanghai, 201206, China.
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Li
authors.affiliation: Thermo Fisher Scientific, Xin Jinqiao Rd., Shanghai, 201206, China.
authors.forename: Zeming
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Thermo Fisher Scientific, Xin Jinqiao Rd., Shanghai, 201206, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Liu
authors.affiliation: Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD, 20892, USA.
authors.forename: Kristopher W
authors.initials: KW
authors.lastname: Krausz
authors.affiliation: Department of Biochemistry and Molecular Medicine, UC Davis Medical Center, Sacramento, CA, 95817, USA.
authors.forename: Aiming
authors.initials: A
authors.lastname: Yu
authors.affiliation: Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD, 20892, USA.
authors.forename: Frank J
authors.initials: FJ
authors.lastname: Gonzalez
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-sen University, 132# Waihuandong Rd, University City of Guangzhou, Guangzhou, 510006, China.
authors.forename: Min
authors.initials: M
authors.lastname: Huang
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-sen University, 132# Waihuandong Rd, University City of Guangzhou, Guangzhou, 510006, China. bihchang@mail.sysu.edu.cn.
authors.forename: Huichang
authors.initials: H
authors.lastname: Bi
mesh_terms: D001120:Arginine / Q000032:analysis / Q000378:metabolism; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor / Q000737:chemistry / Q000378:metabolism* / Q000382:microbiology*; D002853:Chromatography, Liquid; D006801:Humans; D013058:Mass Spectrometry; D055432:Metabolomics / Q000379:methods; D009174:Mycoplasma / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D011687:Purines / Q000032:analysis / Q000378:metabolism
chemical_list: D011687:Purines; D001120:Arginine; C030985:purine
keywords: Cell culture; Cellular metabolism; Metabolomics; Mycoplasma contamination; PANC-1 cells