title: Tissue-specific genome instability in synthetic interspecific hybrids of Pennisetum purpureum (Napier grass) and Pennisetum glaucum (pearl millet) is caused by micronucleation.
abstract: Genome instability is observed in several species hybrids. We studied the mechanisms underlying the genome instability in hexaploid hybrids of Napier grass (Pennisetum purpureum R.) and pearl millet (Pennisetum glaucum L.) using a combination of different methods. Chromosomes of both parental genomes are lost by micronucleation. Our analysis suggests that genome instability occurs preferentially in meristematic root tissue of hexaploid hybrids, and chromosome elimination is not only caused by centromere inactivation. Likely, beside centromere dysfunction, unrepaired DNA double-strand breaks result in fragmented chromosomes in synthetic hybrids.
authors.affiliation: Laboratory of Cytogenetic, Department of Biology, Federal University of Lavras, 37200-000, Lavras, MG, Brazil.|Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) OT Gatersleben, Corrensstr. 3, 06466, Stadt Seeland, Germany.
authors.forename: Gabriela Barreto
authors.initials: GB
authors.lastname: Dos Reis
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) OT Gatersleben, Corrensstr. 3, 06466, Stadt Seeland, Germany. ishii@ipk-gatersleben.de.
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Ishii
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) OT Gatersleben, Corrensstr. 3, 06466, Stadt Seeland, Germany.
authors.forename: Joerg
authors.initials: J
authors.lastname: Fuchs
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) OT Gatersleben, Corrensstr. 3, 06466, Stadt Seeland, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Houben
authors.affiliation: Laboratory of Cytogenetic, Department of Biology, Federal University of Lavras, 37200-000, Lavras, MG, Brazil.
authors.forename: Lisete Chamma
authors.initials: LC
authors.lastname: Davide
mesh_terms: D002503:Centromere / Q000378:metabolism*; D002678:Chimera / Q000235:genetics; D032461:Chromosomes, Plant / Q000235:genetics*; D053903:DNA Breaks, Double-Stranded; D042822:Genomic Instability / Q000235:genetics*; D006824:Hybridization, Genetic; D017404:In Situ Hybridization, Fluorescence; D018519:Meristem / Q000235:genetics*; D048629:Micronuclei, Chromosome-Defective; D031782:Pennisetum / Q000235:genetics*; D000069600:Plant Breeding; D018517:Plant Roots / Q000235:genetics*; D011123:Polyploidy
keywords: Centromere inactivation; Double-strand breaks; Genome instability; Hybrid; Micronuclei