title: Development of a rapid 21-plex autosomal STR typing system for forensic applications.
abstract: DNA-STR genotyping technology has been widely used in forensic investigations. Even with such success, there is a great need to reduce the analysis time. In this study, we established a new rapid 21-plex STR typing system, including 13 CODIS loci, Penta D, Penta E, D12S391, D2S1338, D6S1043, D19S433, D2S441 and Amelogenin loci. This system could shorten the amplification time to a minimum of 90 min and does not require DNA extraction from the samples. Validation of the typing system complied with the Scientific Working Group on DNA Analysis Methods (SWGDAM) and the Chinese National Standard (GA/T815-2009) guidelines. The results demonstrated that this 21-plex STR typing system was a valuable tool for rapid criminal investigation.
authors.affiliation: CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, P. R. China.
authors.forename: Meng
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Forensic Medicine, Nanjing Medical University, Nanjing, Jiangsu, P. R. China.
authors.forename: Caiyong
authors.initials: C
authors.lastname: Yin
authors.affiliation: Beijing Microread Genetics Co., Ltd, Beijing, P. R. China.
authors.forename: Yuexin
authors.initials: Y
authors.lastname: Lv
authors.affiliation: CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, P. R. China.
authors.forename: Yaran
authors.initials: Y
authors.lastname: Yang
authors.affiliation: CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, P. R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: Beijing Microread Genetics Co., Ltd, Beijing, P. R. China.
authors.forename: Zailiang
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Beijing Center for Physical and Chemical Analysis, Beijing, P. R. China.|Beijing Engineering Technology Research Centre of Gene Sequencing and Gene Function Analysis, Beijing, P. R. China.
authors.forename: Xu
authors.initials: X
authors.lastname: Liu
authors.affiliation: Beijing Microread Genetics Co., Ltd, Beijing, P. R. China.
authors.forename: Meibo
authors.initials: M
authors.lastname: Xu
authors.affiliation: Department of Forensic Medicine, Nanjing Medical University, Nanjing, Jiangsu, P. R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.affiliation: Beijing Center for Physical and Chemical Analysis, Beijing, P. R. China. sunnywhj@126.com.|Beijing Engineering Technology Research Centre of Gene Sequencing and Gene Function Analysis, Beijing, P. R. China. sunnywhj@126.com.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Wu
authors.affiliation: CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, P. R. China. yanjw@big.ac.cn.
authors.forename: Jiangwei
authors.initials: J
authors.lastname: Yan
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis / Q000145:classification* / Q000235:genetics*; D053803:Forensic Genetics / Q000379:methods*; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D004247:DNA
keywords: DNA genotyping; Direct amplification; Forensic validation; Rapid PCR; Short tandem repeats