title: Gold nanoprobe functionalized with specific fusion protein selection from phage display and its application in rapid, selective and sensitive colorimetric biosensing of Staphylococcus aureus.
abstract: Staphylococcus aureus (S. aureus) is one of the most ubiquitous pathogens in public healthcare worldwide. It holds great insterest in establishing robust analytical method for S. aureus. Herein, we report a S. aureus-specific recognition element, isolated from phage monoclone GQTTLTTS, which was selected from f8/8 landscape phage library against S. aureus in a high-throughput way. By functionalizing cysteamine (CS)-stabilized gold nanoparticles (CS-AuNPs) with S. aureus-specific pVIII fusion protein (fusion-pVIII), a bifunctional nanoprobe (CS-AuNPs@fusion-pVIII) for S. aureus was developed. In this strategy, the CS-AuNPs@fusion-pVIII could be induced to aggregate quickly in the presence of target S. aureus, resulting in a rapid colorimetric response of gold nanoparticles. More importantly, the as-designed probe exhibited excellent selectivity over other bacteria. Thus, the CS-AuNPs@fusion-pVIII could be used as the indicator of target S. aureus. This assay can detect as low as 19CFUmL(-1)S. aureus within 30min. Further, this approach can be applicable to detect S. aureus in real water samples. Due to its sensitivity, specificity and rapidness, this proposed method is promising for on-site testing of S. aureus without using any costly instruments.
authors.affiliation: Laboratory for Biosensing, Qingdao Institute of Bioenergy & Bioprocess Technology, Chinese Academy of Sciences, Shandong Provincial Key Laboratory of Synthetic Biology, 189 Songling Road, Qingdao 266101, China, and University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China; Institute for Biosensing, and College of Chemical Science and Engineering, Qingdao University, Qingdao 266071, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Liu
authors.affiliation: Laboratory for Biosensing, Qingdao Institute of Bioenergy & Bioprocess Technology, Chinese Academy of Sciences, Shandong Provincial Key Laboratory of Synthetic Biology, 189 Songling Road, Qingdao 266101, China, and University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China; Institute for Biosensing, and College of Chemical Science and Engineering, Qingdao University, Qingdao 266071, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Han
authors.affiliation: Laboratory for Biosensing, Qingdao Institute of Bioenergy & Bioprocess Technology, Chinese Academy of Sciences, Shandong Provincial Key Laboratory of Synthetic Biology, 189 Songling Road, Qingdao 266101, China, and University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China; Institute for Biosensing, and College of Chemical Science and Engineering, Qingdao University, Qingdao 266071, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Pathobiology, Auburn University, 269 Greene Hall, Auburn, AL 36849-5519, United States.
authors.forename: Valery A
authors.initials: VA
authors.lastname: Petrenko
authors.affiliation: Laboratory for Biosensing, Qingdao Institute of Bioenergy & Bioprocess Technology, Chinese Academy of Sciences, Shandong Provincial Key Laboratory of Synthetic Biology, 189 Songling Road, Qingdao 266101, China, and University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China; Institute for Biosensing, and College of Chemical Science and Engineering, Qingdao University, Qingdao 266071, China. Electronic address: liuah@qdu.edu.cn.
authors.forename: Aihua
authors.initials: A
authors.lastname: Liu
mesh_terms: D001435:Bacteriophages / Q000737:chemistry*; D015374:Biosensing Techniques / Q000191:economics / Q000379:methods*; D036022:Capsid Proteins / Q000737:chemistry*; D003124:Colorimetry / Q000191:economics / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D019151:Peptide Library; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000302:isolation & purification* / Q000821:virology; D014871:Water Microbiology
chemical_list: D036022:Capsid Proteins; D019151:Peptide Library; D011993:Recombinant Fusion Proteins; D006046:Gold
keywords: Colorimetric sensor; Gold nanoparticles; Pathogen detection; Specific phage pVIII fusion protein; Staphylococcus aureus