title: Total saponins from dioscorea septemloba thunb reduce serum uric acid levels in rats with hyperuricemia through OATP1A1 up-regulation.
abstract: The aim of this study is to evaluate the efficacy of total saponins of Dioscorea (TSD), an extract of the Chinese herbal Bi Xie, on hyperuricemia and to elucidate the underlying mechanisms. The rat hyperuricemia model was established by administration of adenine. Thirty-two rats were randomly allocated into 4 groups: model group, low/high-dose TSD-treated groups, and allopurinol-treated group. Meanwhile, 8 rats were used as normal controls. Serum uric acid (UA), blood urea nitrogen (BUN), serum creatinine (Scr), and organic anion transporting polypeptide 1A1 (OATP1A1) levels were measured. Comparison between the model group and treatment (allopurinol and TSD) groups showed the serum UA levels were significantly decreased in treatment groups. TSD had similar effects to allopurinol. It was found that the OATP1A1 protein expression levels in treatment groups were higher than in model group and normal controls. And different from the allopurinol-treated groups, TSD-treated group had elevated OATP1A1 expression levels in the stomach, liver, small intestine and large intestine tissues. It was suggested that TSD may facilitate the excretion of UA and lower UA levels by up-regulating OATP1A1 expression.
authors.affiliation: Department of Traditional Chinese Medicine, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Liver Diseases, Traditional Chinese Medical Hospital of Guangdong Province, Guangzhou, 510120, China.
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Chen
authors.affiliation: Department of Traditional Chinese Medicine, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Xiang
authors.affiliation: Department of Traditional Chinese Medicine, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, China.
authors.forename: Bao-Guo
authors.initials: BG
authors.lastname: Sun
authors.affiliation: Department of Traditional Chinese Medicine, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, China.
authors.forename: Hao-Xuan
authors.initials: HX
authors.lastname: Luo
authors.affiliation: Department of Traditional Chinese Medicine, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, China.
authors.forename: Meng-Ting
authors.initials: MT
authors.lastname: Liu
authors.affiliation: Department of Traditional Chinese Medicine, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, China.
authors.forename: Ze-Xiong
authors.initials: ZX
authors.lastname: Chen
authors.affiliation: Department of Traditional Chinese Medicine, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, China. zhsjun1967@hotmail.com.
authors.forename: Shi-Jun
authors.initials: SJ
authors.lastname: Zhang
authors.affiliation: Department of Traditional Chinese Medicine, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangdong Geriatric Institute, Guangzhou, 510080, China. gzwchj@126.com.
authors.forename: Chang-Jun
authors.initials: CJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D003404:Creatinine / Q000097:blood; D027742:Dioscorea / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D005753:Gastric Mucosa / Q000378:metabolism; D033461:Hyperuricemia / Q000188:drug therapy*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D029362:Organic Anion Transporters, Sodium-Independent / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000494:pharmacology* / Q000627:therapeutic use; D013270:Stomach / Q000187:drug effects; D015854:Up-Regulation; D014527:Uric Acid / Q000097:blood*
chemical_list: D004365:Drugs, Chinese Herbal; C000609070:Oatp1a1 protein, rat; D029362:Organic Anion Transporters, Sodium-Independent; D012503:Saponins; D014527:Uric Acid; D003404:Creatinine
keywords: Chinese herb; Dioscorea septemloba Thunb; Dioscoreaceae; hyperuricemia; organic anion transporting polypeptide 1A1