title: Mouse models of amoebiasis and culture methods of amoeba.
abstract: Entamoeba histolytica is the third leading parasitic cause of man mortality in the world. Infection occurs via ingestion of food or water contaminated with cysts of E. histolytica. Amoebae primarily colonize the intestine. The majority of amoebic infections are asymptomatic, but under some conditions, approximately 4-10% of infections progress to the invasive form of the disease. To better understand the pathogenesis of amoebiasis and the interaction between amoebae and their hosts, the development of suitable animal models is crucial. Pigs, gerbils, cats and mice are used as animal models for the study of amoebiasis in the laboratory. Among these, the most commonly used model is the mouse. In addition to intestinal amoebiasis, we developed a mouse model of liver abscess by inoculating amoeba through portal vein. However, the frequency of successful infection remains low, which is dependent on the conditions of amoebae in the laboratory. As the maintenance of virulent amoebae in the laboratory is unstable, it needs further refinement. This review summarizes mouse models of amoebiasis and the current state of laboratory culture method of amoebae.
authors.affiliation: Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan; Doctoral Leadership Program, Graduate School of Biomedical Science, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
authors.forename: Sharmina
authors.initials: S
authors.lastname: Deloer
authors.affiliation: Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan; Doctoral Leadership Program, Graduate School of Biomedical Science, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
authors.forename: Risa
authors.initials: R
authors.lastname: Nakamura
authors.affiliation: Divisions of Molecular and Cellular Immunoscience, Department of Biomolecular Sciences, Faculty of Medicine, Saga University, Saga 849-8501, Japan.
authors.forename: Fumika
authors.initials: F
authors.lastname: Mi-Ichi
authors.affiliation: Department of Immunology, Yamaguchi University Graduate School of Medicine, Ube 755-8505, Japan.
authors.forename: Keishi
authors.initials: K
authors.lastname: Adachi
authors.affiliation: Graduate School of Medicine, Department of Infectious Diseases, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Seiki
authors.initials: S
authors.lastname: Kobayashi
authors.affiliation: Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan; Doctoral Leadership Program, Graduate School of Biomedical Science, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan. Electronic address: shinjiro@nagasaki-u.ac.jp.
authors.forename: Shinjiro
authors.initials: S
authors.lastname: Hamano
keywords: Amoebiasis; Culture method; Mouse models