title: The fatty acid chain elongase, Elovl1, is required for kidney and swim bladder development during zebrafish embryogenesis.
abstract: Very long chain fatty acids are required for sphingolipid synthesis, lipid homeostasis, myelin formation, epidermal permeability, and retinal function. Seven different enzymes are known to be involved in the elongation cycle of fatty acids, with different chain-length specificities. Elovl1 is one of those enzymes whose function has been linked mainly to the synthesis of sphingolipids and the epidermal barrier. However, the role of Elovl1 in organogenesis is not clear. In zebrafish, 2 Elovl1 genes, elovl1a and elovl1b, are highly expressed in the swim bladder, and elovl1b is also expressed in the kidney. We found that both elovl1 knockdown embryos contain increased levels of long chain fatty acids from carbon number 14 to 20 as compared to control embryos. Oil-Red-O staining shows that yolk lipid consumption is greatly reduced, whereas lipid droplets accumulate within the swim bladder. Notably, knockdown of either elovl1a or elovl1b affects the expression of genes involved in swim bladder development and impairs inflation of the swim bladder. Consistent with its expression in the pronephros, knockdown of elovl1b alone affects the expression of genes required for kidney development and reduces renal clearance. Our findings strongly suggest that both elovl1 genes are a key determinant of swim bladder and kidney development in zebrafish, which may be comparatively applicable to lung and kidney development in humans.
authors.affiliation: a Department of Microbiology and Center for Metabolic Function Regulation, Wonkwang University School of Medicine , Iksan , Jeonbuk , Republic of Korea.
authors.forename: Sushil
authors.initials: S
authors.lastname: Bhandari
authors.affiliation: a Department of Microbiology and Center for Metabolic Function Regulation, Wonkwang University School of Medicine , Iksan , Jeonbuk , Republic of Korea.
authors.forename: Joon No
authors.initials: JN
authors.lastname: Lee
authors.affiliation: a Department of Microbiology and Center for Metabolic Function Regulation, Wonkwang University School of Medicine , Iksan , Jeonbuk , Republic of Korea.
authors.forename: Young-Il
authors.initials: YI
authors.lastname: Kim
authors.affiliation: a Department of Microbiology and Center for Metabolic Function Regulation, Wonkwang University School of Medicine , Iksan , Jeonbuk , Republic of Korea.
authors.forename: In-Koo
authors.initials: IK
authors.lastname: Nam
authors.affiliation: b Asan Institute of Life Sciences, University of Ulsan College of Medicine, Asan Medical Center , Seoul , Republic of Korea.
authors.forename: Su-Jung
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: a Department of Microbiology and Center for Metabolic Function Regulation, Wonkwang University School of Medicine , Iksan , Jeonbuk , Republic of Korea.
authors.forename: Se-Jin
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: c Zoonosis Research Center, Wonkwang University School of Medicine , Iksan , Jeonbuk , Republic of Korea.
authors.forename: SeongAe
authors.initials: S
authors.lastname: Kwak
authors.affiliation: a Department of Microbiology and Center for Metabolic Function Regulation, Wonkwang University School of Medicine , Iksan , Jeonbuk , Republic of Korea.
authors.forename: Gi-Su
authors.initials: GS
authors.lastname: Oh
authors.affiliation: a Department of Microbiology and Center for Metabolic Function Regulation, Wonkwang University School of Medicine , Iksan , Jeonbuk , Republic of Korea.
authors.forename: Hyung-Jin
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: b Asan Institute of Life Sciences, University of Ulsan College of Medicine, Asan Medical Center , Seoul , Republic of Korea.
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Yoo
authors.affiliation: a Department of Microbiology and Center for Metabolic Function Regulation, Wonkwang University School of Medicine , Iksan , Jeonbuk , Republic of Korea.
authors.forename: Hong-Seob
authors.initials: HS
authors.lastname: So
authors.affiliation: a Department of Microbiology and Center for Metabolic Function Regulation, Wonkwang University School of Medicine , Iksan , Jeonbuk , Republic of Korea.|d Institute of Wonkwang Medical Science, Wonkwang University School of Medicine , Iksan , Jeonbuk , Republic of Korea.
authors.forename: Seong-Kyu
authors.initials: SK
authors.lastname: Choe
authors.affiliation: a Department of Microbiology and Center for Metabolic Function Regulation, Wonkwang University School of Medicine , Iksan , Jeonbuk , Republic of Korea.|e Department of Biomedical Science & Engineering , Institute of Integrated Technology, Gwangju Institute of Science & Technology , Gwangju , Republic of Korea.
authors.forename: Raekil
authors.initials: R
authors.lastname: Park
mesh_terms: D000123:Acetyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000400:Air Sacs / Q000196:embryology* / Q000201:enzymology*; D000595:Amino Acid Sequence; D000818:Animals; D017124:Conserved Sequence; D004530:Egg Yolk / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000378:metabolism; D047108:Embryonic Development / Q000235:genetics; D020440:Gene Duplication; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D016678:Genome; D007668:Kidney / Q000196:embryology* / Q000201:enzymology* / Q000502:physiology; D050356:Lipid Metabolism; D008322:Mammals; D009186:Myelin Sheath / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D029961:Zebrafish Proteins; D000123:Acetyltransferases; C000624687:elovl1a protein, zebrafish
keywords: fatty acid chain elongase; kidney development; swim bladder development; zebrafish