title: Recombinant Escherichia coli K5 strain with the deletion of waaR gene decreases the molecular weight of the heparosan capsular polysaccharide.
abstract: Heparosan, the capsular polysaccharide of Escherichia coli K5 having a carbohydrate backbone similar to that of heparin, has become a potential precursor for bioengineering heparin. In the heparosan biosynthesis pathway, the gene waaR encoding α-1-, 2- glycosyltransferase catalyze s the third glucosyl residues linking to the oligosaccharide chain. In the present study, a waaR deletion mutant of E. coli K5 was constructed. The mutant showed improvement of capsule polysaccharide yield. It is interesting that the heparosan molecular weight of the mutant is reduced and may become more suitable as a precursor for the production of low molecular weight heparin derived from the wild-type K5 capsular polysaccharide.
authors.affiliation: College of Biological Engineering, Zhejiang University of Technology, Hangzhou, 310032, China.
authors.forename: Haichan
authors.initials: H
authors.lastname: Huang
authors.affiliation: College of Biological Engineering, Zhejiang University of Technology, Hangzhou, 310032, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Biological Engineering, Zhejiang University of Technology, Hangzhou, 310032, China.
authors.forename: Shencong
authors.initials: S
authors.lastname: Lv
authors.affiliation: College of Biological Engineering, Zhejiang University of Technology, Hangzhou, 310032, China. whzhong@zjut.edu.cn.
authors.forename: Weihong
authors.initials: W
authors.lastname: Zhong
authors.affiliation: Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.
authors.forename: Fuming
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.|Department of Biological Science, Departments of Chemistry and Chemical Biology and Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Linhardt
mesh_terms: D016667:Bacterial Capsules / Q000737:chemistry / Q000378:metabolism*; D004187:Disaccharides / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D008970:Molecular Weight
chemical_list: D004187:Disaccharides; D029968:Escherichia coli Proteins; C560363:heparosan; D005964:Glucosyltransferases; C000613736:WaaR protein, E coli
keywords: Capsular polysaccharide; Escherichia coli K5; Gene waaR; Low molecular weight heparin