title: Interaction of GoxA with Its Modifying Enzyme and Its Subunit Assembly Are Dependent on the Extent of Cysteine Tryptophylquinone Biosynthesis.
abstract: GoxA is a glycine oxidase bearing a protein-derived cysteine tryptophylquinone (CTQ) cofactor that is formed by posttranslational modifications catalyzed by a flavoprotein, GoxB. Two forms of GoxA were isolated: an active form with mature CTQ and an inactive precursor protein that lacked CTQ. The active GoxA was present as a homodimer with no detectable affinity for GoxB, whereas the precursor was isolated as a monomer in a tight complex with one GoxB. Thus, the interaction of GoxA with GoxB and subunit assembly of mature GoxA are each dependent on the extent of CTQ biosynthesis.
authors.affiliation: Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida , Orlando, Florida 32827, United States.
authors.forename: Esha
authors.initials: E
authors.lastname: Sehanobish
authors.affiliation: Department of Genetics and Microbiology, University of Murcia , Murcia 30100, Spain.
authors.forename: Jonatan C
authors.initials: JC
authors.lastname: Campillo-Brocal
authors.affiliation: Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida , Orlando, Florida 32827, United States.
authors.forename: Heather R
authors.initials: HR
authors.lastname: Williamson
authors.affiliation: Department of Genetics and Microbiology, University of Murcia , Murcia 30100, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Sanchez-Amat
authors.affiliation: Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida , Orlando, Florida 32827, United States.
authors.forename: Victor L
authors.initials: VL
authors.lastname: Davidson
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004151:Dipeptides / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D045563:Indolequinones / Q000737:chemistry / Q000378:metabolism*; D053544:Marinomonas / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D060066:Protein Interaction Maps; D055503:Protein Multimerization; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004151:Dipeptides; D045563:Indolequinones; D011994:Recombinant Proteins; C447619:cysteine tryptophylquinone; D000594:Amino Acid Oxidoreductases; C115925:glycine oxidase