title: A novel optical biosensor for direct and selective determination of serotonin in serum by Solid Surface-Room Temperature Phosphorescence.
abstract: This paper describes a novel biosensor which combines the use of nanotechnology (non-woven nanofibre mat) with Solid Surface-Room Temperature Phosphorescence (SS-RTP) measurement for the determination of serotonin in human serum. The developed biosensor is simple and can be directly applied in serum; only requires a simple clean-up protocol. Therefore it is the first time that serotonin is analysed directly in serum with a non-enzymatic technique. This new approach is based on the covalent immobilization of serotonin directly from serum on a functional nanofibre material (Tiss®-Link) with a preactivated surface for direct covalent immobilization of primary and secondary amines, and the subsequent measurement of serotonin phosphorescent emission from the solid surface. The phosphorescent detection allows avoiding the interference from any fluorescence emission or scattering light from any molecule present in the serum sample which can be also immobilised on the nanofibre material. The determination of serotonin with this SS-RTP sensor overcomes some limitations, such as large interference from the matrix and high cost and complexity of many of the methods widely used for serotonin analysis. The potential applicability of the sensor in the clinical diagnosis was demonstrated by analysing serum samples from seven healthy volunteers. The method was validated with an external reference laboratory, obtaining a correlation coefficient of 0.997 which indicates excellent correlation between the two methods.
authors.affiliation: Department of Analytical Chemistry, University of Granada, Avd. Fuentenueva s/n, 18071 Granada, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Ramon-Marquez
authors.affiliation: NanoMyP®, Nanomateriales y Polimeros S.L., Spin-Off Company of the UGR, BIC Building, Avd. Innovacion 1, E-18016 Granada, Spain. Electronic address: amedina@nanomyp.com.
authors.forename: Antonio L
authors.initials: AL
authors.lastname: Medina-Castillo
authors.affiliation: Department of Analytical Chemistry, University of Granada, Avd. Fuentenueva s/n, 18071 Granada, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Fernandez-Gutierrez
authors.affiliation: Department of Analytical Chemistry, University of Granada, Avd. Fuentenueva s/n, 18071 Granada, Spain. Electronic address: jffernan@ugr.es.
authors.forename: Jorge F
authors.initials: JF
authors.lastname: Fernandez-Sanchez
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D006801:Humans; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods*; D057139:Nanofibers / Q000737:chemistry; D036103:Nanotechnology; D012701:Serotonin / Q000097:blood*; D013696:Temperature
chemical_list: D012701:Serotonin
keywords: Nanofibre mat; Nanotechnology; Phosphorescence; Protein clean-up; SS-RTP; Serotonin; Serum; Tiss®-Link