title: Assembly and Assessment of DNA Scaffolded Vaccines.
abstract: Vaccines play an important role in preventing many life-threatening infectious diseases. To meet the demand of vaccination for treating a wide range of diseases, rational vaccine design has been recognized as a desirable and necessary strategy for development of safe and effective vaccines. DNA nanostructures are advantageous in the design and construction of synthetic vaccines, owing to their robust self-assembly, programmability, and precision control in complex organization, as well as their intrinsic adjuvant activity. Here, we describe a modular assembly of DNA scaffolded vaccine complex, composing of a model antigen, streptavidin, and adjuvant, CpG oligonucleotide. The DNA-assembled vaccines were found to elicit strong antigen-specific antibody responses, but causing little or no adverse reactions. Conceivably, this vaccine platform can be further optimized for improved immunogenicity and extended to the construction of various subunit vaccines.
authors.affiliation: Center for Infectious Diseases and Vaccinology, The Biodesign Institute, Arizona State University, Tempe, AZ, 85287, USA.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University, Chengdu, Sichuan Province, 610041, China.|Department of Stomatology, Sichuan Provincial People's Hospital, Sichuan Academy of Medical Sciences, Chengdu, Sichuan, 610101, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.affiliation: Center for Molecular Design and Biomimetics, The Biodesign Institute, Arizona State University, Tempe, AZ, 85287, USA.|Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ, 85287, USA.
authors.forename: Hao
authors.initials: H
authors.lastname: Yan
authors.affiliation: Center for Infectious Diseases and Vaccinology, The Biodesign Institute, Arizona State University, Tempe, AZ, 85287, USA. yung.chang@asu.edu.|School of Life Sciences, Arizona State University, Tempe, AZ, 85287, USA. yung.chang@asu.edu.
authors.forename: Yung
authors.initials: Y
authors.lastname: Chang
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry; D000818:Animals; D000906:Antibodies / Q000276:immunology; D001692:Biological Transport; D005260:Female; D005434:Flow Cytometry; D007114:Immunization; D051379:Mice; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D000067996:RAW 264.7 Cells; D019809:Streptavidin / Q000737:chemistry; D019444:Vaccines, DNA / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism
chemical_list: D000276:Adjuvants, Immunologic; D000906:Antibodies; C408982:CPG-oligonucleotide; D009838:Oligodeoxyribonucleotides; D019444:Vaccines, DNA; D019809:Streptavidin
keywords: Adjuvant; DNA nanostructures; Self-assembly; Synthetic vaccine