title: Early exposure of rotating magnetic fields promotes central nervous regeneration in planarian Girardia sinensis.
abstract: Magnetic field exposure is an accepted safe and effective modality for nerve injury. However, it is clinically used only as a supplement or salvage therapy at the later stage of treatment. Here, we used a planarian Girardia sinensis decapitated model to investigate beneficial effects of early rotary non-uniform magnetic fields (RMFs) exposure on central nervous regeneration. Our results clearly indicated that magnetic stimulation induced from early RMFs exposure significantly promoted neural regeneration of planarians. This stimulating effect is frequency and intensity dependent. Optimum effects were obtained when decapitated planarians were cultured at 20 °C, starved for 3 days before head-cutting, and treated with 6 Hz 0.02 T RMFs. At early regeneration stage, RMFs exposure eliminated edema around the wound and facilitated subsequent formation of blastema. It also accelerated cell proliferation and recovery of neuron functionality. Early RMFs exposure up-regulated expression of neural regeneration related proteins, EGR4 and Netrin 2, and mature nerve cell marker proteins, NSE and NPY. These results suggest that RMFs therapy produced early and significant benefit in central nervous regeneration, and should be clinically used at the early stage of neural regeneration, with appropriate optimal frequency and intensity.
authors.affiliation: The Engineering Lab of Synthetic Biology and the Key Lab of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen, P. R. China.|Key Laboratory of Optoelectronics Devices and Systems of Ministry of Education, Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, P. R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
authors.affiliation: The Engineering Lab of Synthetic Biology and the Key Lab of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen, P. R. China.
authors.forename: Gui-miao
authors.initials: GM
authors.lastname: Lin
authors.affiliation: The Engineering Lab of Synthetic Biology and the Key Lab of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen, P. R. China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wu
authors.affiliation: The Engineering Lab of Synthetic Biology and the Key Lab of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen, P. R. China.
authors.forename: Sheng-wei
authors.initials: SW
authors.lastname: Tang
authors.affiliation: The Engineering Lab of Synthetic Biology and the Key Lab of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen, P. R. China.
authors.forename: Zhi-jia
authors.initials: ZJ
authors.lastname: Zheng
authors.affiliation: The Engineering Lab of Synthetic Biology and the Key Lab of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen, P. R. China.
authors.forename: Marie Chia-mi
authors.initials: MC
authors.lastname: Lin
authors.affiliation: Key Laboratory of Optoelectronics Devices and Systems of Ministry of Education, Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, P. R. China.
authors.forename: Gai-xia
authors.initials: GX
authors.lastname: Xu
authors.affiliation: The Engineering Lab of Synthetic Biology and the Key Lab of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen, P. R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Laboratory of Optoelectronics Devices and Systems of Ministry of Education, Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, P. R. China.
authors.forename: Yue-yue
authors.initials: YY
authors.lastname: Deng
authors.affiliation: The Engineering Lab of Synthetic Biology and the Key Lab of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen, P. R. China.
authors.forename: Xiao-yun
authors.initials: XY
authors.lastname: Zhang
authors.affiliation: The Engineering Lab of Synthetic Biology and the Key Lab of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen, P. R. China.
authors.forename: Si-ping
authors.initials: SP
authors.lastname: Chen
authors.affiliation: The Engineering Lab of Synthetic Biology and the Key Lab of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen, P. R. China.
authors.forename: Xiao-mei
authors.initials: XM
authors.lastname: Wang
authors.affiliation: Key Laboratory of Optoelectronics Devices and Systems of Ministry of Education, Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, P. R. China.
authors.forename: Han-ben
authors.initials: HB
authors.lastname: Niu
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002490:Central Nervous System / Q000502:physiology*; D005786:Gene Expression Regulation; D060526:Magnetic Fields; D009416:Nerve Regeneration; D010932:Planarians / Q000502:physiology*; D012399:Rotation; D013696:Temperature; D013997:Time Factors
chemical_list: D015415:Biomarkers
keywords: neural regeneration; planarian; rotary non-uniform magnetic fields; stimulating effect; window effect