title: Short-Fragment DNA Residue from Vaccine Purification Processes Promotes Immune Response to the New Inactivated EV71 Vaccine by Upregulating TLR9 mRNA.
abstract: To reduce potential oncogenic long genomic DNA in vaccines, nuclease treatment has been applied in the purification processes. However, this action increased the residue of short-fragment DNA and its effect on vaccine potency was still elusive. In this study, we found residual sf-DNA in an inactivated EV71 vaccine could enhance humoral immune response in mice. Ag stimulation in vitro and vaccine injection in vivo revealed that TLR9 transcription level was elevated, indicating that sf-DNA could activate TLR9. These new findings will help us to understand the molecular mechanism induced by vero-cell culture-derived vaccines.
authors.affiliation: School of Life Sciences, Jilin University, Changchun, P.R. China.|National Institutes for Food and Drug Control, Beijing, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Shao
authors.affiliation: National Institutes for Food and Drug Control, Beijing, P.R. China.
authors.forename: Fan
authors.initials: F
authors.lastname: Gao
authors.affiliation: National Institutes for Food and Drug Control, Beijing, P.R. China.
authors.forename: Hui-Juan
authors.initials: HJ
authors.lastname: Lin
authors.affiliation: National Institutes for Food and Drug Control, Beijing, P.R. China.
authors.forename: Qun-Ying
authors.initials: QY
authors.lastname: Mao
authors.affiliation: National Institutes for Food and Drug Control, Beijing, P.R. China.
authors.forename: Pan
authors.initials: P
authors.lastname: Chen
authors.affiliation: National Institutes for Food and Drug Control, Beijing, P.R. China.
authors.forename: Xing
authors.initials: X
authors.lastname: Wu
authors.affiliation: National Institutes for Food and Drug Control, Beijing, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yao
authors.affiliation: School of Life Sciences, Jilin University, Changchun, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Kong
authors.affiliation: National Institutes for Food and Drug Control, Beijing, P.R. China.
authors.forename: Zheng-Lun
authors.initials: ZL
authors.lastname: Liang
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D002522:Chlorocebus aethiops; D018899:CpG Islands; D004247:DNA / Q000737:chemistry*; D029821:Enterovirus A, Human; D004769:Enterovirus Infections / Q000276:immunology*; D005260:Female; D056724:Immunity, Humoral; D007274:Injections, Intraperitoneal; D008214:Lymphocytes / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013154:Spleen / Q000378:metabolism; D051217:Toll-Like Receptor 9 / Q000378:metabolism*; D015854:Up-Regulation; D015164:Vaccines, Inactivated / Q000276:immunology; D014709:Vero Cells; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D012333:RNA, Messenger; D051217:Toll-Like Receptor 9; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; D004247:DNA