title: Enhancement of anti-inflammatory activity of bromelain by its encapsulation in katira gum nanoparticles.
abstract: Bromelain-loaded katira gum nanoparticles were synthesized using 3 level optimization process and desirability approach. Nanoparticles of the optimized batch were characterized using particle size analysis, zeta potential, transmission electron microscopy and Fourier-transform infrared spectroscopy. Investigation of their in vivo anti-inflammatory activity by employing carrageenan induced rat-paw oedema method showed that encapsulation of bromelain in katira gum nanoparticles substantially enhanced its anti-inflammatory potential. This may be attributed to enhanced absorption owing to reduced particle size or to protection of bromelain from acid proteases.
authors.affiliation: Department of Bio & NanoTechnology, Guru Jambheshwar University of Science & Technology, Hisar, Haryana 125001, India.
authors.forename: Manju
authors.initials: M
authors.lastname: Bernela
authors.affiliation: Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science & Technology, Hisar, Haryana 125001, India.
authors.forename: Munish
authors.initials: M
authors.lastname: Ahuja
authors.affiliation: Department of Bio & NanoTechnology, Guru Jambheshwar University of Science & Technology, Hisar, Haryana 125001, India. Electronic address: rthakur99@gmail.com.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Thakur
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000627:therapeutic use*; D031212:Bixaceae / Q000737:chemistry*; D001963:Bromelains / Q000737:chemistry / Q000627:therapeutic use*; D002122:Calcium Chloride; D002351:Carrageenan; D004008:Diclofenac / Q000627:therapeutic use; D004339:Drug Compounding; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D005260:Female; D015636:Magnesium Chloride; D008297:Male; D053758:Nanoparticles; D010316:Particle Size; D053149:Plant Gums / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D053149:Plant Gums; D015636:Magnesium Chloride; D004008:Diclofenac; D002351:Carrageenan; D001963:Bromelains; C091760:stem bromelain; D002122:Calcium Chloride
keywords: Bromelain; Cochlospermum religiosum; FTIR; Katira gum; Nanoparticles