title: Establishment of chondroitin B lyase-based analytical methods for sensitive and quantitative detection of dermatan sulfate in heparin.
abstract: Dermatan sulfate (DS) is one of the hardest impurities to remove from heparin products due to their high structural similarity. The development of a sensitive and feasible method for quantitative detection of DS in heparin is essential to ensure the clinical safety of heparin pharmaceuticals. In the current study, based on the substrate specificity of chondroitin B lyase, ultraviolet spectrophotometric and strong anion-exchange high-performance liquid chromatographic methods were established for detection of DS in heparin. The former method facilitated analysis in heparin with DS concentrations greater than 0.1mgmL(-1) at 232nm, with good linearity, precision and recovery. The latter method allowed sensitive and accurate detection of DS at concentrations lower than 0.1mgmL(-1), exhibiting good linearity, precision and recovery. The linear range of DS detection using the latter method was between 0.01 and 0.5mgmL(-1).
authors.affiliation: Key Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, People's Republic of China; Product Research and Development Center, Yichang Humanwell Pharmaceutical Co., Ltd., No.19, Dalian Road, Yichang Development Zone, Yichang, Hubei 443005, People's Republic of China. Electronic address: wujingjun2009@foxmail.com.
authors.forename: Jingjun
authors.initials: J
authors.lastname: Wu
authors.affiliation: Key Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, People's Republic of China. Electronic address: jiyang0326@163.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Ji
authors.affiliation: Key Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, People's Republic of China. Electronic address: sunan12345@126.com.
authors.forename: Nan
authors.initials: N
authors.lastname: Su
authors.affiliation: Department of Biotechnology, Beijing Electronic Science and Technology Vocational College, 1A Shaoyaoju, Chaoyang, Beijing 100029, People's Republic of China. Electronic address: liyeyashi@163.com.
authors.forename: Ye
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Biotechnology, Beijing Electronic Science and Technology Vocational College, 1A Shaoyaoju, Chaoyang, Beijing 100029, People's Republic of China. Electronic address: liuxinxin0412@163.com.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, People's Republic of China. Electronic address: meixianggoodman@126.com.
authors.forename: Xiang
authors.initials: X
authors.lastname: Mei
authors.affiliation: Key Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, People's Republic of China. Electronic address: yoyo_qian@126.com.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Key Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, People's Republic of China. Electronic address: chongzhang@mail.tsinghua.edu.cn.
authors.forename: Chong
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, People's Republic of China. Electronic address: xhxing@tsinghua.edu.cn.
authors.forename: Xin-hui
authors.initials: XH
authors.lastname: Xing
mesh_terms: D002808:Chondroitin Lyases / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003871:Dermatan Sulfate / Q000032:analysis*; D004187:Disaccharides / Q000032:analysis; D006493:Heparin / Q000737:chemistry*; D007474:Ion Exchange; D057230:Limit of Detection; D016014:Linear Models; D058105:Polymerization; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D004187:Disaccharides; D003871:Dermatan Sulfate; D006493:Heparin; D002808:Chondroitin Lyases
keywords: Chondroitin B lyase; Dermatan sulfate; Heparin; High performance liquid chromatography; Ultraviolet spectrophotometry