title: [Anti-depressive mechanism of Fufang Chaigui prescription based on neuroendocrine hormone and metabolomic correlation analysis].
abstract: To elucidate the anti-depressive effect of Fufang Chaigui prescription and its mechanism and investigate its effect on neuroendocrine hormone, rats were included into a chronic unpredictable mild stress (CUMS) model for 28 d, and drugs were administered at the same time. During the period, rats' behaviors were observed and the blood was collected by using ELISA to determine representative hormone concentrations of HPAA, HPTA and HPGA. The changes in endogenous metabolites were analyzed by using H NMR metabolomics to seek the potential biomarkers. Results showed Fufang Chaigui prescription could improve the behaviors of CUMS rats obviously, increase contents of ACTH, CORT, T₃and decrease contents of TSH and TESTO and regulate the levels of lactate, α-glucose, choline, N-acetylglycoprotein, trimethylamine oxide and leucine to get closer to the contents of control group. The results of correlation analysis indicated that HPTA was associated with glycometabolism, amino acid metabolism and choline metabolism. And HPAA was related to glycometabolism and amino acid metabolism. However, HPGA was only correlated with glycometabolism. In conclusion, Fufang Chaigui prescription could show an obvious anti-depressive effect and its underlying mechanism might involve regulations of neuroendocrine function and pathways of glycometabolism, amino acid metabolism and choline metabolism.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Huan
authors.initials: H
authors.lastname: Liu
authors.forename: Jian-li
authors.initials: JL
authors.lastname: Chen
authors.forename: Xiao-xia
authors.initials: XX
authors.lastname: Gao
authors.forename: Yu-zhi
authors.initials: YZ
authors.lastname: Zhou
authors.forename: Jun-sheng
authors.initials: JS
authors.lastname: Tian
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Qin
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000008:administration & dosage*; D001522:Behavior, Animal; D003863:Depression / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D006728:Hormones / Q000378:metabolism*; D006801:Humans; D008297:Male; D055432:Metabolomics; D009490:Neurosecretory Systems / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D044967:Serum / Q000737:chemistry / Q000378:metabolism
chemical_list: D000928:Antidepressive Agents; D004365:Drugs, Chinese Herbal; D006728:Hormones