title: Intracellular uptake and fluorescence imaging potential in tumor cell of zinc phthalocyanine.
abstract: A near IR absorbing phthalocyanine bearing four binaphtyl group has been synthesized in order to investigate its cytotoxicity and intracellular uptake of sensitizer on MCF-7 (human breast cancer), MDAH (ovarian cancer), HeLa (human epitheloid cervix carcinoma), EMT-6 (mouse breast cancer) and WI-38 (human fibroblast lung) cell lines. ZnPc showed four time higher intracellular uptake in carcinoma cells (MCF-7) than normal (WI-38) cell lines. With the aim of studying in detail the biodistribution feature and tumor nuclear imaging capacity, ZnPc was also labeled with I-131. The efficiency of radiolabeled compound was 95±4.6%. In addition, ZnPc reveals to be very efficient singlet oxygen generators (ΦΔ=0.612 in DMSO) and promising PS for PDT application. In vitro fluorescence imaging study with MCF-7 cells showed that ZnPc localized in cytoplasm of the cells. This results showed that synthesized ZnPc is promising candidate for dual fluorescence/nuclear imaging breast cancer and shows potential PS for PDT application.
authors.affiliation: Science and Letters Faculty, Chemistry Department, Mersin University, Ciftlikkoy Campus, TR-33343 Yenisehir, Mersin, Turkey.
authors.forename: Göktürk
authors.initials: G
authors.lastname: Avşar
authors.affiliation: Advanced Technology Research & Application Center, Mersin University, Ciftlikkoy Campus, TR-33343 Mersin, Turkey.
authors.forename: Fatma Aslıhan
authors.initials: FA
authors.lastname: Sari
authors.affiliation: Advanced Technology Research & Application Center, Mersin University, Ciftlikkoy Campus, TR-33343 Mersin, Turkey.
authors.forename: A Celil
authors.initials: AC
authors.lastname: Yuzer
authors.affiliation: Department of Biomedical Technology, Institute of Science, Ege University, Bornova, 35100, Izmir, Turkey.
authors.forename: Hale Melis
authors.initials: HM
authors.lastname: Soylu
authors.affiliation: Department of Nuclear Applications, Institute of Nuclear Science, Ege University, Bornova, 35100, Izmir, Turkey.
authors.forename: Ozge
authors.initials: O
authors.lastname: Er
authors.affiliation: Advanced Technology Research & Application Center, Mersin University, Ciftlikkoy Campus, TR-33343 Mersin, Turkey; Department of Energy Systems Engineering Faculty of Tarsus Technology, Mersin University, 33480 Mersin, Turkey. Electronic address: mine.ince@mersin.edu.tr.
authors.forename: Mine
authors.initials: M
authors.lastname: Ince
authors.affiliation: Department of Nuclear Applications, Institute of Nuclear Science, Ege University, Bornova, 35100, Izmir, Turkey. Electronic address: fatma.yurt.lambrecht@ege.edu.tr.
authors.forename: Fatma Yurt
authors.initials: FY
authors.lastname: Lambrecht
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism; D045744:Cell Line, Tumor; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D006801:Humans; D007211:Indoles / Q000008:administration & dosage* / Q000138:chemical synthesis / Q000493:pharmacokinetics; D007457:Iodine Radioisotopes; D054833:Isoindoles; D061986:MCF-7 Cells; D051379:Mice; D061848:Optical Imaging / Q000379:methods; D009942:Organometallic Compounds / Q000008:administration & dosage* / Q000138:chemical synthesis / Q000493:pharmacokinetics; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000008:administration & dosage* / Q000138:chemical synthesis / Q000493:pharmacokinetics; D026082:Singlet Oxygen / Q000378:metabolism; D014018:Tissue Distribution; D017967:Zinc Compounds
chemical_list: D007211:Indoles; D007457:Iodine Radioisotopes; D054833:Isoindoles; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine; D026082:Singlet Oxygen
keywords: (131)I; Breast cancer; Dual imaging; Nuclear imaging; Phthalocyanine; Radiolabeling