title: Enhancements of the production of bilirubin and the expression of β-globin by carbon monoxide during erythroid differentiation.
abstract: Heme is degraded by heme oxygenase to form iron, carbon monoxide (CO), and biliverdin. However, information about the catabolism of heme in erythroid cells is limited. In this study, we showed the production and export of bilirubin in murine erythroleukemia (MEL) cells. The production of bilirubin by MEL cells was enhanced when heme synthesis was induced. When mouse bone marrow cells were induced with erythropoietin to differentiate into erythroid cells, the synthesis of bilirubin increased. The expression of β-globin was enhanced by CO at the transcriptional level. These results indicate that constant production of CO from heme regulates erythropoiesis.
authors.affiliation: Department of Biotechnology, Kyoto Institute of Technology, Japan.
authors.forename: Anfeng
authors.initials: A
authors.lastname: Mu
authors.affiliation: Unit of Research Complex, Kansai Medical University, Hirakara, Osaka, Japan.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: Unit of Research Complex, Kansai Medical University, Hirakara, Osaka, Japan.
authors.forename: Masakazu
authors.initials: M
authors.lastname: Tanaka
authors.affiliation: Department of Pathology, Toyooka Hospital, Hyogo, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Adachi
authors.affiliation: Department of Biotechnology, Kyoto Institute of Technology, Japan.|Department of Physiology, Pathophysiology and Immunology, Pham Ngoc Thach University of Medicine, Ho Chi Minh, Vietnam.
authors.forename: Tran Tien
authors.initials: TT
authors.lastname: Tai
authors.affiliation: Department of Biotechnology, Kyoto Institute of Technology, Japan.|Department of Plastic and Aesthetic Surgery, Pham Ngoc Thach University of Medicine, Ho Chi Minh, Vietnam.
authors.forename: Pham Hieu
authors.initials: PH
authors.lastname: Liem
authors.affiliation: Department of Biotechnology, Kyoto Institute of Technology, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Izawa
authors.affiliation: Department of Molecular Biochemistry, Iwate Medical University, Yahaba, Iwate, Japan.
authors.forename: Kazumichi
authors.initials: K
authors.lastname: Furuyama
authors.affiliation: Department of Biotechnology, Kyoto Institute of Technology, Japan.|Unit of Research Complex, Kansai Medical University, Hirakara, Osaka, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Taketani
mesh_terms: D000818:Animals; D001663:Bilirubin / Q000378:metabolism*; D001854:Bone Marrow Cells; D002248:Carbon Monoxide / Q000494:pharmacology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D041905:Erythroid Cells / Q000166:cytology* / Q000378:metabolism; D004920:Erythropoiesis; D005786:Gene Expression Regulation / Q000187:drug effects; D006418:Heme / Q000378:metabolism; D051379:Mice; D055544:beta-Globins / Q000378:metabolism*
chemical_list: D055544:beta-Globins; D006418:Heme; D002248:Carbon Monoxide; D001663:Bilirubin
keywords: bilirubin; bone marrow cells; carbon monoxide; heme biosynthesis; hemoglobin