title: Dense EM-based reconstruction of the interglomerular projectome in the zebrafish olfactory bulb.
abstract: The dense reconstruction of neuronal circuits from volumetric electron microscopy (EM) data has the potential to uncover fundamental structure-function relationships in the brain. To address bottlenecks in the workflow of this emerging methodology, we developed a procedure for conductive sample embedding and a pipeline for neuron reconstruction. We reconstructed ∼98% of all neurons (>1,000) in the olfactory bulb of a zebrafish larva with high accuracy and annotated all synapses on subsets of neurons representing different types. The organization of the larval olfactory bulb showed marked differences from that of the adult but similarities to that of the insect antennal lobe. Interneurons comprised multiple types but granule cells were rare. Interglomerular projections of interneurons were complex and bidirectional. Projections were not random but biased toward glomerular groups receiving input from common types of sensory neurons. Hence, the interneuron network in the olfactory bulb exhibits a specific topological organization that is governed by glomerular identity.
authors.affiliation: Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.|University of Basel, Basel, Switzerland.
authors.forename: Adrian A
authors.initials: AA
authors.lastname: Wanner
authors.affiliation: Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
authors.forename: Christel
authors.initials: C
authors.lastname: Genoud
authors.affiliation: Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.|University of Basel, Basel, Switzerland.
authors.forename: Tafheem
authors.initials: T
authors.lastname: Masudi
authors.affiliation: Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
authors.forename: Léa
authors.initials: L
authors.lastname: Siksou
authors.affiliation: Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.|University of Basel, Basel, Switzerland.
authors.forename: Rainer W
authors.identifier: 0000-0001-9107-0482
authors.initials: RW
authors.lastname: Friedrich
mesh_terms: D000818:Animals; D007395:Interneurons / Q000648:ultrastructure*; D007814:Larva / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods; D009415:Nerve Net / Q000378:metabolism; D009830:Olfactory Bulb / Q000648:ultrastructure*; D011984:Sensory Receptor Cells / Q000648:ultrastructure*; D013569:Synapses / Q000378:metabolism*; D015027:Zebrafish