title: The Brain Metabolome of Male Rats across the Lifespan.
abstract: Comprehensive and accurate characterization of brain metabolome is fundamental to brain science, but has been hindered by technical limitations. We profiled the brain metabolome in male Wistar rats at different ages (day 1 to week 111) using high-sensitivity and high-resolution mass spectrometry. Totally 380 metabolites were identified and 232 of them were quantitated. Compared with anatomical regions, age had a greater effect on variations in the brain metabolome. Lipids, fatty acids and amino acids accounted for the largest proportions of the brain metabolome, and their concentrations varied across the lifespan. The levels of polyunsaturated fatty acids were higher in infancy (week 1 to week 3) compared with later ages, and the ratio of omega-6 to omega-3 fatty acids increased in the aged brain (week 56 to week 111). Importantly, a panel of 20 bile acids were quantitatively measured, most of which have not previously been documented in the brain metabolome. This study extends the breadth of the mammalian brain metabolome as well as our knowledge of functional brain development, both of which are critically important to move the brain science forward.
authors.affiliation: Shanghai Key Laboratory of Diabetes Mellitus and Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China.
authors.forename: Xiaojiao
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Shanghai Key Laboratory of Diabetes Mellitus and Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China.
authors.forename: Tianlu
authors.initials: T
authors.lastname: Chen
authors.affiliation: Shanghai Key Laboratory of Diabetes Mellitus and Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Zhao
authors.affiliation: Ministry of Education Key Laboratory of Systems Biomedicine, Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
authors.affiliation: University of Hawaii Cancer Center, Honolulu 96813, USA.
authors.forename: Guoxiang
authors.initials: G
authors.lastname: Xie
authors.affiliation: Shanghai Key Laboratory of Diabetes Mellitus and Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China.
authors.forename: Fengjie
authors.initials: F
authors.lastname: Huang
authors.affiliation: Shanghai Key Laboratory of Diabetes Mellitus and Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China.
authors.forename: Jiajian
authors.initials: J
authors.lastname: Liu
authors.affiliation: Shanghai Key Laboratory of Diabetes Mellitus and Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Shanghai Key Laboratory of Diabetes Mellitus and Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China.
authors.forename: Shouli
authors.initials: S
authors.lastname: Wang
authors.affiliation: Ministry of Education Key Laboratory of Systems Biomedicine, Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Chongchong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Center for Chinese Medical Therapy and Systems Biology, E-Institute, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Mingmei
authors.initials: M
authors.lastname: Zhou
authors.affiliation: University of Hawaii Cancer Center, Honolulu 96813, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Panee
authors.affiliation: F. M. Kirby Neurobiology Center, Children's Hospital, and Department of Neurology, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: He
authors.affiliation: Shanghai Key Laboratory of Diabetes Mellitus and Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China.|University of Hawaii Cancer Center, Honolulu 96813, USA.|Center for Chinese Medical Therapy and Systems Biology, E-Institute, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Jia
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001647:Bile Acids and Salts / Q000032:analysis; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D016000:Cluster Analysis; D015525:Fatty Acids, Omega-3 / Q000032:analysis; D043371:Fatty Acids, Omega-6 / Q000032:analysis; D016018:Least-Squares Analysis; D008136:Longevity; D008297:Male; D013058:Mass Spectrometry; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000596:Amino Acids; D001647:Bile Acids and Salts; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6