title: Characterization of matrix effects in developing rugged high-throughput LC-MS/MS methods for bioanalysis.
abstract: OBJECTIVE
  There is an ever-increasing demand for high-throughput LC-MS/MS bioanalytical assays to support drug discovery and development.
  
  
  RESULTS
  Matrix effects of sofosbuvir (protonated) and paclitaxel (sodiated) were thoroughly evaluated using high-throughput chromatography (defined as having a run time ≤1 min) under 14 elution conditions with extracts from protein precipitation, liquid-liquid extraction and solid-phase extraction. A slight separation, in terms of retention time, between underlying matrix components and sofosbuvir/paclitaxel can greatly alleviate matrix effects.
  
  
  CONCLUSIONS
  High-throughput chromatography, with proper optimization, can provide rapid and effective chromatographic separation under 1 min to alleviate matrix effects and enhance assay ruggedness for regulated bioanalysis.
authors.affiliation: PPD® Laboratories, 3230 Deming Way, Middleton, WI 53562, USA.
authors.forename: Fumin
authors.initials: F
authors.lastname: Li
authors.affiliation: PPD® Laboratories, 3230 Deming Way, Middleton, WI 53562, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: PPD® Laboratories, 2240 Dabney Rd, Richmond, VA 23230, USA.
authors.forename: Rand
authors.initials: R
authors.lastname: Jenkins
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000097:blood* / Q000302:isolation & purification; D000998:Antiviral Agents / Q000097:blood* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000191:economics / Q000379:methods; D006801:Humans; D059625:Liquid-Liquid Extraction / Q000191:economics / Q000379:methods; D017239:Paclitaxel / Q000097:blood* / Q000302:isolation & purification; D000069474:Sofosbuvir / Q000097:blood* / Q000302:isolation & purification; D052616:Solid Phase Extraction / Q000191:economics / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000191:economics / Q000379:methods*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000998:Antiviral Agents; D017239:Paclitaxel; D000069474:Sofosbuvir
keywords: high-throughput LC–MS/MS methods; matrix effects; paclitaxel; regulated bioanalysis; sofosbuvir; sub-minute chromatography