title: In vitro Effect of Mouthrinse Containing Essential Oils on Proliferation and Migration of Gingival Epithelial Cells.
abstract: We aimed to investigate in vitro the effects of mouthrinses containing essential oils (EOs) on proliferation and migration of gingival epithelial cells. Human gingival epithelial cells were treated with predetermined dilutions of commercially available EO mouthrinses with or without ethanol and a mouthrinse containing cetyl pyridinium chloride (CPC) for 60 s. Cell proliferation was evaluated using WST-1 assay. Cell migration was assessed using a wound closure model. Within 10 s of exposure to EO mouthrinse without ethanol, the epithelial cells became aberrant and shrank. No statistically significant difference in cell migration or proliferation was observed among cells pretreated by the EO mouthrinse with ethanol, CPC mouthrinse and control (phosphate buffered saline). In contrast, the EO mouthrinse without ethanol significantly reduced cell proliferation (p < 0.001) to approximately 20% relative to control. As for the EO mouthrinse without ethanol, it was not possible to assess its effect on cell migration using this model, because treated cells could be easily detached from the culture plate upon scratch, possibly because of the surfactant ingredient in the formulation. Within the limitations of the study, the EO mouthrinse with ethanol exerted no inhibitory effect on proliferation and migration of the gingival epithelial cells. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Periodontology, Tokyo Dental College, Tokyo, Japan.
authors.forename: Kouki
authors.initials: K
authors.lastname: Yoshikawa
authors.affiliation: Department of Periodontology, Tokyo Dental College, Tokyo, Japan.
authors.forename: Jin
authors.initials: J
authors.lastname: Sekino
authors.affiliation: Department of Periodontology, Tokyo Dental College, Tokyo, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Imamura
authors.affiliation: Department of Periodontology, Tokyo Dental College, Tokyo, Japan.|Oral Health Science Center, Tokyo Dental College, Japan.
authors.forename: Koki
authors.initials: K
authors.lastname: Ota
authors.affiliation: Department of Periodontology, Tokyo Dental College, Tokyo, Japan.
authors.forename: Daichi
authors.initials: D
authors.lastname: Kita
authors.affiliation: Department of Periodontology, Tokyo Dental College, Tokyo, Japan.|Oral Health Science Center, Tokyo Dental College, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Saito
mesh_terms: D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology; D005881:Gingiva / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D009067:Mouthwashes / Q000494:pharmacology*; D009822:Oils, Volatile / Q000494:pharmacology*
chemical_list: D009067:Mouthwashes; D009822:Oils, Volatile; D000431:Ethanol
keywords: cell migration; essential oils; gingival epithelial cells; mouthrinse; periodontal disease