title: Evaluation of capacity to detect ability to form biofilm in Candida parapsilosis sensu stricto strains by MALDI-TOF MS.
abstract: Matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is, currently, used as a rapid and reliable tool in microbial diagnostics. The discriminatory power of the method extends its applicability also beyond species level. This study examined the possibility to use MALDI-TOF MS to differentiate between Candida parapsilosis sensu stricto biofilm-positive (n = 12) and biofilm-negative (n = 9) strains. The results indicated a grouping trend within MALDI-TOF mass spectra belonging to each of the tested groups. However, these trends were eclipsed by mass spectral variations resulting from limited repeatability of the method, making its application for the selected purpose impossible. Improvement in the discriminatory power of the method was not obtained neither by using different matrices (α-cyano-4-hydroxycinnamic acid, ferulic acid, 5-chloro-2-mercaptobenzothionazole) for MALDI-TOF MS analysis nor by testing different culture conditions (cultivation length, culture media).
authors.affiliation: Department of Microbiology, Faculty of Medicine, Masaryk University, Kamenice 5, Brno, 62500, Czech Republic.
authors.forename: Katarína
authors.initials: K
authors.lastname: Mlynáriková
authors.affiliation: Research Group Proteomics, Central European Institute of Technology, Masaryk University, Kamenice 5, 62500, Brno, Czech Republic.|National Centre of Biomolecular Research, Faculty of Science, Masaryk University, Kamenice 5, 62500, Brno, Czech Republic.
authors.forename: Ondrej
authors.initials: O
authors.lastname: Šedo
authors.affiliation: Department of Microbiology, Faculty of Medicine, Masaryk University, Kamenice 5, Brno, 62500, Czech Republic. fruzic@fnusa.cz.|Department of Microbiology, St. Anne's Faculty Hospital, Pekarska 53, 65691, Brno, Czech Republic. fruzic@fnusa.cz.
authors.forename: Filip
authors.initials: F
authors.lastname: Růžička
authors.affiliation: Research Group Proteomics, Central European Institute of Technology, Masaryk University, Kamenice 5, 62500, Brno, Czech Republic.|National Centre of Biomolecular Research, Faculty of Science, Masaryk University, Kamenice 5, 62500, Brno, Czech Republic.
authors.forename: Zbyněk
authors.initials: Z
authors.lastname: Zdráhal
authors.affiliation: Department of Microbiology, Faculty of Medicine, Masaryk University, Kamenice 5, Brno, 62500, Czech Republic.|Department of Microbiology, St. Anne's Faculty Hospital, Pekarska 53, 65691, Brno, Czech Republic.
authors.forename: Veronika
authors.initials: V
authors.lastname: Holá
authors.affiliation: Department of Microbiology, Faculty of Medicine, Masaryk University, Kamenice 5, Brno, 62500, Czech Republic.
authors.forename: Martina
authors.initials: M
authors.lastname: Mahelová
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D002175:Candida / Q000737:chemistry* / Q000502:physiology*; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*