title: Up-Regulation of GABAergic Signal Events in Bone Marrow Lymphocytes in Childhood Acute Lymphoblastic Leukemia.
abstract: Gamma-aminobutyric acid (GABA) is involved in the proliferation, differentiation, and migration of several cell types including cancer cells. Whether GABA may be involved with acute lymphoblastic leukemia (ALL) is unclear. Therefore, the goal of this report was to examine if GABAergic signaling expression is altered in bone marrow lymphocytes of ALL children. RT-PCR and western blot analysis were used to examine the expression of the GABA synthetizing enzyme glutamic acid decarboxylase (GAD) isoforms (GAD65 and GAD67), and type-A GABA receptor (GABAAR) subunits [α(1-6), β(1-3), γ(1-3), δ, ε, θ, π, and ρ(1-3)] in bone marrow lymphocytes of 19 ALL children before chemotherapy. The data obtained were compared with those in 13 age-matched non-ALL children. Immunofluorescent staining was used to examine the cellular localization of GAD. We found that GAD and GABAAR subunits were expressed in bone marrow lymphocytes of ALL children. Moreover, RT-PCR and western blot showed that GAD and several GABAAR subunits were significantly increased in ALL children as compared with the data of non-ALL children. Our present study reveals up-regulation of GABAergic signaling events in bone marrow lymphocytes in ALL children. However, the role of this signaling system in lymphocyte proliferation and invasion as related to the progression of ALL requires further investigation.
authors.affiliation: Department of Pediatric Hematology and Oncology, Qian fo shan Hospital Affiliated to Shandong University, 16766#, Jing-shi Road, Jinan 250012.|Department of Pediatric Hematology and Oncology, Provincial Hospital Affiliated to Shandong University, 324#, Jing-wu Wei-qi Road, Jinan 250021.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Wang
authors.affiliation: The Central Laboratory of Provincial Hospital Affiliated to Shandong University, 324#, Jing-wu Wei-qi Road, Jinan 250021.
authors.forename: Mei
authors.initials: M
authors.lastname: Feng
authors.affiliation: Shandong Provincial Key Laboratory of Radiation Oncology, Shandong Cancer Hospital and Institute, 440#, Ji Yan Road, Jinan 250117.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Physiology, Shandong University School of Medicine, 44#, Wenhua Xi Road, Jinan 250012.
authors.forename: Chuanfei
authors.initials: C
authors.lastname: Wei
authors.affiliation: Department of Physiology, Shandong University School of Medicine, 44#, Wenhua Xi Road, Jinan 250012.
authors.forename: Mengjun
authors.initials: M
authors.lastname: Qian
authors.affiliation: Department of Physiology, Shandong University School of Medicine, 44#, Wenhua Xi Road, Jinan 250012.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Physiology, Shandong University School of Medicine, 44#, Wenhua Xi Road, Jinan 250012.
authors.forename: Xupeng
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Physiology, Shandong University School of Medicine, 44#, Wenhua Xi Road, Jinan 250012.
authors.forename: Shuang
authors.initials: S
authors.lastname: Cui
authors.affiliation: Department of Physiology, Shandong University School of Medicine, 44#, Wenhua Xi Road, Jinan 250012, Shandong, People's Republic of China.
authors.forename: Chuanyong
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Pharmacology, Shandong University School of Medicine, 44#, Wenhua Xi Road, Jinan 250012, Shandong, People's Republic of China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yi
authors.affiliation: Department of Physiology, Shandong University School of Medicine, 44#, Wenhua Xi Road, Jinan 250012, Shandong, People's Republic of China.
authors.forename: Shuanglian
authors.initials: S
authors.lastname: Wang
mesh_terms: D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000187:drug effects*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D005968:Glutamate Decarboxylase / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000201:enzymology / Q000378:metabolism*; D011963:Receptors, GABA-A / Q000096:biosynthesis / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects; D005680:gamma-Aminobutyric Acid / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase