title: Efficient therapeutic delivery by a novel cell-permeant peptide derived from KDM4A protein for antitumor and antifibrosis.
abstract: Cell-penetrating peptide (CPP) based delivery have provided immense potential for the therapeutic applications, however, most of nonhuman originated CPPs carry the risk of possible cytotoxicity and immunogenicity, thus may restricting to be used. Here, we describe a novel human-derived CPP, denoted hPP10, and hPP10 has cell-penetrating properties evaluated by CellPPD web server, as well as In-Vitro and In-Vivo analysis. In vitro studies showed that hPP10-FITC was able to penetrate into various cells including primary cultured cells, likely through an endocytosis pathway. And functionalized macromolecules, such as green fluorescent protein (GFP), tumor-specific apoptosis inducer Apoptin as well as biological active enzyme GCLC (Glutamate-cysteine ligase, catalytic subunit) can be delivered by hPP10 in vitro and in vivo. Collectively, our results suggest that hPP10 provide a novel and versatile tool to deliver exogenous proteins or drugs for clinical applications as well as reprogrammed cell-based therapy.
authors.affiliation: The Institute of Cell Therapy, China Three Gorges University, Yichang 443002, China.|Medical School, China Three Gorges University, Yichang 443002, China.|Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang 443002, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Wang
authors.affiliation: Medical School, China Three Gorges University, Yichang 443002, China.
authors.forename: Jie-Lan
authors.initials: JL
authors.lastname: Ma
authors.affiliation: The Institute of Cell Therapy, China Three Gorges University, Yichang 443002, China.|Medical School, China Three Gorges University, Yichang 443002, China.
authors.forename: Ying-Gui
authors.initials: YG
authors.lastname: Yang
authors.affiliation: The Institute of Cell Therapy, China Three Gorges University, Yichang 443002, China.|Medical School, China Three Gorges University, Yichang 443002, China.|Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang 443002, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Song
authors.affiliation: The Institute of Cell Therapy, China Three Gorges University, Yichang 443002, China.|Medical School, China Three Gorges University, Yichang 443002, China.|Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang 443002, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Wu
authors.affiliation: Medical School, China Three Gorges University, Yichang 443002, China.|Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang 443002, China.
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Qin
authors.affiliation: Medical School, China Three Gorges University, Yichang 443002, China.|Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang 443002, China.
authors.forename: Xue-Li
authors.initials: XL
authors.lastname: Zhao
authors.affiliation: The Institute of Cell Therapy, China Three Gorges University, Yichang 443002, China.|The 1st People's Hospital of Yichang, Yichang 443000, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: The Institute of Cell Therapy, China Three Gorges University, Yichang 443002, China.|Medical School, China Three Gorges University, Yichang 443002, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Zou
authors.affiliation: The Institute of Cell Therapy, China Three Gorges University, Yichang 443002, China.|Medical School, China Three Gorges University, Yichang 443002, China.
authors.forename: Jiang-Feng
authors.initials: JF
authors.lastname: Wu
authors.affiliation: The Institute of Cell Therapy, China Three Gorges University, Yichang 443002, China.|The 1st People's Hospital of Yichang, Yichang 443000, China.
authors.forename: Jun-Ming
authors.initials: JM
authors.lastname: Li
authors.affiliation: The Institute of Cell Therapy, China Three Gorges University, Yichang 443002, China.|Medical School, China Three Gorges University, Yichang 443002, China.|Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang 443002, China.
authors.forename: Chang-Bai
authors.initials: CB
authors.lastname: Liu
mesh_terms: D000072283:A549 Cells; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D057846:Cell-Penetrating Peptides / Q000494:pharmacology*; D016503:Drug Delivery Systems / Q000379:methods; D004705:Endocytosis; D005355:Fibrosis; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D056484:Jumonji Domain-Containing Histone Demethylases / Q000494:pharmacology*; D046911:Macromolecular Substances; D008545:Melanoma / Q000188:drug therapy / Q000378:metabolism; D008546:Melanoma, Experimental; D051379:Mice; D010455:Peptides / Q000494:pharmacology; D021381:Protein Transport
chemical_list: D000970:Antineoplastic Agents; D057846:Cell-Penetrating Peptides; D046911:Macromolecular Substances; D010455:Peptides; D049452:Green Fluorescent Proteins; D056484:Jumonji Domain-Containing Histone Demethylases; C494424:KDM4A protein, human
keywords: GFP; anti-tumor; cell-penetrating peptides (CPPs); internalization; melanoma