title: Conjugated Linoleic Triacylglycerols Exhibit Superior Lymphatic Absorption Than Free Conjugate Linoleic Acids and Have Antiobesity Properties.
abstract: This study aimed to compare lymphatic absorption of conjugated linoleic acids (CLAs) in the triacylglycerol (TAG) or free fatty acid (FFA) form and to examine the antiobesity effects of different doses of CLAs in the TAG form in animals. Conjugated linoleic TAGs (containing 70.3 wt% CLAs; CLA-TAG) were prepared through lipase-catalyzed esterification of glycerol with commercial CLA mixtures (CLA-FFA). Lymphatic absorption of CLA-TAG and CLA-FFA was compared in a rat model of lymphatic cannulation. Greater amounts of cis-9,trans-11 and trans-10,cis-12 CLAs were detected in the collected lymph from a lipid emulsion containing CLA-TAG. This result suggests that CLA-TAG has greater capacity for lymphatic absorption than does CLA-FFA. The antiobesity efficacy of CLA-TAG at different doses was examined in mice with diet-induced obesity. A high-fat diet (HFD) for 12 weeks caused a significant increase in body weight and epididymal and retroperitoneal fat weights, which were significantly decreased by 2% dietary supplementation (w/w) with CLA-TAG. CLA-TAG at 2% significantly attenuated the HFD-induced upregulation of serum TAG, but led to hepatomegaly and exacerbated HFD-induced hypercholesterolemia. CLA-TAG at 1% significantly attenuated upregulation of retroperitoneal fat weight and significantly increased liver weight, which was decreased by the HFD. Nonetheless, the liver weight in group "HFD +1% CLA-TAG" was not significantly different from that of normal diet controls. CLA-TAG at 1% significantly reduced serum TAG levels and did not exacerbate HFD-induced hypercholesterolemia. Thus, 1% dietary supplementation with CLA-TAG reduces retroperitoneal fat weight without apparent hepatomegaly, a known side-effect of CLAs in mouse models of obesity.
authors.affiliation: 1 Department of Food Science and Technology, Chung-Ang University , Anseong, Korea.
authors.forename: Hyunjoon
authors.initials: H
authors.lastname: Woo
authors.affiliation: 2 Korea Food Research Institute , Seongnam, Korea.
authors.forename: Min-Yu
authors.initials: MY
authors.lastname: Chung
authors.affiliation: 3 Department of Food and Nutrition, Changwon National University , Changwon, Korea.
authors.forename: Juyeon
authors.initials: J
authors.lastname: Kim
authors.affiliation: 3 Department of Food and Nutrition, Changwon National University , Changwon, Korea.
authors.forename: Daecheol
authors.initials: D
authors.lastname: Kong
authors.affiliation: 2 Korea Food Research Institute , Seongnam, Korea.
authors.forename: Jinyoung
authors.initials: J
authors.lastname: Min
authors.affiliation: 2 Korea Food Research Institute , Seongnam, Korea.
authors.forename: Hee-Don
authors.initials: HD
authors.lastname: Choi
authors.affiliation: 2 Korea Food Research Institute , Seongnam, Korea.
authors.forename: In-Wook
authors.initials: IW
authors.lastname: Choi
authors.affiliation: 4 Department of Food and Nutrition, Korea University , Seoul, Korea.
authors.forename: In-Hwan
authors.initials: IH
authors.lastname: Kim
authors.affiliation: 3 Department of Food and Nutrition, Changwon National University , Changwon, Korea.
authors.forename: Sang K
authors.initials: SK
authors.lastname: Noh
authors.affiliation: 5 Department of Food and Nutrition, Sookmyung Women's University , Seoul, Korea.
authors.forename: Byung Hee
authors.initials: BH
authors.lastname: Kim
mesh_terms: D000818:Animals; D019440:Anti-Obesity Agents / Q000008:administration & dosage* / Q000737:chemistry; D059305:Diet, High-Fat / Q000009:adverse effects; D005230:Fatty Acids, Nonesterified; D006801:Humans; D044243:Linoleic Acids, Conjugated / Q000008:administration & dosage* / Q000737:chemistry; D008099:Liver / Q000187:drug effects / Q000254:growth & development; D008196:Lymph / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000188:drug therapy* / Q000378:metabolism; D009929:Organ Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000008:administration & dosage / Q000737:chemistry*
chemical_list: D019440:Anti-Obesity Agents; D005230:Fatty Acids, Nonesterified; D044243:Linoleic Acids, Conjugated; D014280:Triglycerides
keywords: antiobesity; conjugated linoleic triacylglycerol; high-fat diet; lymphatic absorption