title: Overexpression of Dishevelled-2 contributes to proliferation and migration of human esophageal squamous cell carcinoma.
abstract: Dishevelled-2 (Dvl2) was associated with tumor cell proliferation and migration. We aimed to examine the mechanism of Dvl2 in esophageal squamous cell carcinoma (ESCC). Dvl2 was overexpressed in human ESCC tissues and cell lines ECA109 and TE1 cells. CCK-8 and colony formation assay was performed to evaluate the proliferation in ECA109 cells transfected with Dvl2-shRNA. Wound-healing assay and transwell assay were used to examine the activities of migration and invasion in Dvl2-silenced ESCC cells. Knockdown of Dvl2 significantly reduced ECA109 cell proliferation and migration. Moreover, we demonstrated that the proliferation and migration ability of Dvl2 might through the activation of Wnt pathway by targeting the Cyclin D1 and MMP-9. We came to the conclusion that the proliferation and migration effects of Dvl2 might contribute to malignant development of human ESCC.
authors.affiliation: Department of Chemotherapy, Jiangsu Cancer Hospital Affiliated to Nanjing Medical University, 42 Baiziting Raod, Nanjing, 210000, Jiangsu, China.
authors.forename: Guoren
authors.initials: G
authors.lastname: Zhou
authors.affiliation: Department of Radiotherapy, Jiangsu Cancer Hospital Affiliated to Nanjing Medical University, 42 Baiziting Raod, Nanjing, 210000, Jiangsu, China. jjye2004@163.com.
authors.forename: Jinjun
authors.initials: J
authors.lastname: Ye
authors.affiliation: Department of Radiotherapy, Jiangsu Cancer Hospital Affiliated to Nanjing Medical University, 42 Baiziting Raod, Nanjing, 210000, Jiangsu, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Chemotherapy, Jiangsu Cancer Hospital Affiliated to Nanjing Medical University, 42 Baiziting Raod, Nanjing, 210000, Jiangsu, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Chemotherapy, Jiangsu Cancer Hospital Affiliated to Nanjing Medical University, 42 Baiziting Raod, Nanjing, 210000, Jiangsu, China. 791207933@QQ.com.
authors.forename: Jifeng
authors.initials: J
authors.lastname: Feng
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism; D000072261:Dishevelled Proteins / Q000235:genetics* / Q000378:metabolism; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011379:Prognosis; D047368:Tumor Burden; D060449:Wnt Signaling Pathway
chemical_list: D000072261:Dishevelled Proteins
keywords: Dvl2; Esophageal squamous cell carcinoma; Migration; Proliferation; Wnt signaling activation