title: Clinical sensitivity of HPV assays for the detection of high grade cervical disease in cervical samples treated with glacial acetic acid.
abstract: Lysis of bloody liquid based cytology (LBC) specimens with glacial acetic acid (GAA) is performed to aid cytological interpretation. However, the influence of GAA treatment on HPV detection is not fully understood and in studies designed to assess this, few cases of high-grade disease have been included.
  
  
  
  To assess the sensitivity of HPV molecular tests for the detection of high grade cervical disease in GAA treated samples
  
  
  
  A total of 207 specimens associated with high grade dyskaryosis and treated with GAA were collated prospectively. Overall 140 specimens had underlying CIN2+, including 88 CIN3. All specimens were tested with the Abbott RealTime High Risk HPV test (rtHPV) and the Qiagen Hybrid Capture 2High Risk HPV DNA test (HC2). Specimens associated with a CIN2+ that were negative by either assay were genotyped.
  
  
  
  The sensitivity of rtHPV for CIN2+ and CIN3+ was 92.8% (87.2, 96.5) and 94.3% (87.2, 98.1) respectively. Sensitivity of the HC2 for CIN2+ and CIN3+ was 97.2% (92.8, 99.2) and 96.6% (90.3, 99.2) respectively. The sensitivity of both assays in GAA treated specimens was thus consistent with the level required for clinical application. HPV negative, CIN2+ specimens were generally attributable to HPV types outside the explicit analytical range of the assays.
  
  
  
  The data indicate that GAA treatment has little impact on the detection of CIN2+ by HPV testing in LBC specimens.
authors.affiliation: Scottish HPV Reference Laboratory, Royal Infirmary of Edinburgh, Edinburgh, UK.
authors.forename: Catherine
authors.initials: C
authors.lastname: Moore
authors.affiliation: Department of Pathology, Royal Infirmary of Edinburgh, Edinburgh, UK.
authors.forename: Edward
authors.initials: E
authors.lastname: Duvall
authors.affiliation: Department of Pathology, Royal Infirmary of Edinburgh, Edinburgh, UK.
authors.forename: Ellen
authors.initials: E
authors.lastname: Braby
authors.affiliation: Department of Pathology, Queen Elizabeth University Hospital, Glasgow, UK.
authors.forename: Graham
authors.initials: G
authors.lastname: Reid
authors.affiliation: Department of Pathology, Queen Elizabeth University Hospital, Glasgow, UK.
authors.forename: Eileen
authors.initials: E
authors.lastname: Docherty
authors.affiliation: Scottish HPV Reference Laboratory, Royal Infirmary of Edinburgh, Edinburgh, UK.
authors.forename: Logan
authors.initials: L
authors.lastname: Grieve
authors.affiliation: HPV Research Group, Queens Medical Research Institute, University of Edinburgh, Edinburgh, UK.
authors.forename: Heather
authors.initials: H
authors.lastname: Cubie
authors.affiliation: Epidemiology and Statistics Core, Wellcome Trust Clinical Research Facility, Edinburgh, UK.
authors.forename: Cat
authors.initials: C
authors.lastname: Graham
authors.affiliation: Scottish HPV Reference Laboratory, Royal Infirmary of Edinburgh, Edinburgh, UK. Electronic address: Kate.Cuschieri@luht.scot.nhs.uk.
authors.forename: Kate
authors.initials: K
authors.lastname: Cuschieri
mesh_terms: D019342:Acetic Acid / Q000008:administration & dosage*; D002584:Cervix Uteri / Q000821:virology*; D005260:Female; D006801:Humans; D007202:Indicators and Reagents / Q000008:administration & dosage*; D025202:Molecular Diagnostic Techniques / Q000379:methods; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000150:complications / Q000175:diagnosis*; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000821:virology
chemical_list: D007202:Indicators and Reagents; D019342:Acetic Acid
keywords: Cervical; Glacial acetic acid; HPV; Molecular detection