title: Sensitive analysis and pharmacokinetic study of the isomers paeoniflorin and albiflorin after oral administration of Total Glucosides Of White Paeony Capsule in rats.
abstract: A sensitive and reliable method using liquid chromatography tandem mass spectrometry (LC-MS/MS) was established for the simultaneous assay of paeoniflorin and albiflorin in bio-samples of rats after liquid-liquid extraction with ethylacetate. For the first time, the developed method was validated and successfully applied to the pharmacokinetics study of paeoniflorin and albiflorin after oral administration of Total Glucosides Of White Paeony Capsule (TGP). Relative to the intravenous injection, the absolute bio-availabilities of paeoniflorin and albiflorin were 2.8 and 1.7%, while their excretion in feces was 43.06 and 40.87%, respectively. Both paeoniflorin and albiflorin showed dose-dependent exposure in plasma, with a half-life of approximately 1.8h. No significant differences were observed between a single equal dose of paeoniflorin or albiflorin and that of TGP for the pharmacokinetic parameters, including AUC, T1/2 and Cmax. Paeoniflorin and albiflorin were exposed at high levels in immune relevant organ/tissues, such as the spleen, thymus and bone, which could facilitate immuno-regulatory activities.
authors.affiliation: Jiangsu Province Key Laboratory of Drug Metabolism and Pharmacokinetics, Jiangsu Key laboratory of drug design and optimization, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Tongjiaxiang 24, Nanjing 210009, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Fei
authors.affiliation: Lansen Pharmaceutical Holdings Co. Ltd, Ningbo 315174, PR China.
authors.forename: Hailing
authors.initials: H
authors.lastname: Yang
authors.affiliation: Jiangsu Province Key Laboratory of Drug Metabolism and Pharmacokinetics, Jiangsu Key laboratory of drug design and optimization, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Tongjiaxiang 24, Nanjing 210009, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Jiangsu Province Key Laboratory of Drug Metabolism and Pharmacokinetics, Jiangsu Key laboratory of drug design and optimization, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Tongjiaxiang 24, Nanjing 210009, PR China.
authors.forename: Pei
authors.initials: P
authors.lastname: Wang
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, PR China.
authors.forename: Shuyao
authors.initials: S
authors.lastname: Wang
authors.affiliation: Jiangsu Province Key Laboratory of Drug Metabolism and Pharmacokinetics, Jiangsu Key laboratory of drug design and optimization, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Tongjiaxiang 24, Nanjing 210009, PR China.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Jiangsu Province Key Laboratory of Drug Metabolism and Pharmacokinetics, Jiangsu Key laboratory of drug design and optimization, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Tongjiaxiang 24, Nanjing 210009, PR China.
authors.forename: Jingqiu
authors.initials: J
authors.lastname: Huang
authors.affiliation: Jiangsu Province Key Laboratory of Drug Metabolism and Pharmacokinetics, Jiangsu Key laboratory of drug design and optimization, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Tongjiaxiang 24, Nanjing 210009, PR China.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Yu
authors.affiliation: Jiangsu Province Key Laboratory of Drug Metabolism and Pharmacokinetics, Jiangsu Key laboratory of drug design and optimization, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Tongjiaxiang 24, Nanjing 210009, PR China.
authors.forename: Siqi
authors.initials: S
authors.lastname: Feng
authors.affiliation: Jiangsu Province Key Laboratory of Drug Metabolism and Pharmacokinetics, Jiangsu Key laboratory of drug design and optimization, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Tongjiaxiang 24, Nanjing 210009, PR China.
authors.forename: Runbin
authors.initials: R
authors.lastname: Sun
authors.affiliation: Jiangsu Province Key Laboratory of Drug Metabolism and Pharmacokinetics, Jiangsu Key laboratory of drug design and optimization, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Tongjiaxiang 24, Nanjing 210009, PR China.
authors.forename: Na
authors.initials: N
authors.lastname: Yang
authors.affiliation: Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Pharmacy School at Yantai University, Yantai 264005, PR China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Wang
authors.affiliation: Jiangsu Province Key Laboratory of Drug Metabolism and Pharmacokinetics, Jiangsu Key laboratory of drug design and optimization, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Tongjiaxiang 24, Nanjing 210009, PR China. Electronic address: jiyea@cpu.edu.cn.
authors.forename: Jiye
authors.initials: J
authors.lastname: Aa
authors.affiliation: Jiangsu Province Key Laboratory of Drug Metabolism and Pharmacokinetics, Jiangsu Key laboratory of drug design and optimization, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Tongjiaxiang 24, Nanjing 210009, PR China. Electronic address: guangjiwang@hotmail.com.
authors.forename: Guangji
authors.initials: G
authors.lastname: Wang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001952:Bridged-Ring Compounds / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D002853:Chromatography, Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics; D005260:Female; D005960:Glucosides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D007536:Isomerism; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D039821:Monoterpenes / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D029596:Paeonia / Q000737:chemistry*; D051381:Rats; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods; D006027:Glycosides
chemical_list: D001952:Bridged-Ring Compounds; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D039821:Monoterpenes; C015423:peoniflorin; D006027:Glycosides; C014959:albiflorin
keywords: Albiflorin; LC–MS/MS; Paeoniflorin; Pharmacokinetics; Tissue distribution