title: Immortalized endothelial cell lines for in vitro blood-brain barrier models: A systematic review.
abstract: Endothelial cells play the most important role in construction of the blood-brain barrier. Many studies have opted to use commercially available, easily transfected or immortalized endothelial cell lines as in vitro blood-brain barrier models. Numerous endothelial cell lines are available, but we do not currently have strong evidence for which cell lines are optimal for establishment of such models. This review aimed to investigate the application of immortalized endothelial cell lines as in vitro blood-brain barrier models. The databases used for this review were PubMed, OVID MEDLINE, ProQuest, ScienceDirect, and SpringerLink. A narrative systematic review was conducted and identified 155 studies. As a result, 36 immortalized endothelial cell lines of human, mouse, rat, porcine and bovine origins were found for the establishment of in vitro blood-brain barrier and brain endothelium models. This review provides a summary of immortalized endothelial cell lines as a guideline for future studies and improvements in the establishment of in vitro blood-brain barrier models. It is important to establish a good and reproducible model that has the potential for multiple applications, in particular a model of such a complex compartment such as the blood-brain barrier.
authors.affiliation: Pengiran Anak Puteri Rashidah Saadatul Bolkiah Institute of Health Sciences, Universiti Brunei Darussalam, Brunei Darussalam. Electronic address: 14h8153@ubd.edu.bn.
authors.forename: Nurul Adhwa
authors.initials: NA
authors.lastname: Rahman
authors.affiliation: Pengiran Anak Puteri Rashidah Saadatul Bolkiah Institute of Health Sciences, Universiti Brunei Darussalam, Brunei Darussalam.
authors.forename: Alifah Nur'ain Haji Mat
authors.initials: ANHM
authors.lastname: Rasil
authors.affiliation: Department of Neurology, University of Heidelberg, Germany; Department of Neurology, Krankenhaus Nordwest, Frankfurt, Germany; The Brunei Neuroscience Stroke and Rehabilitation Centre, Brunei Darussalam.
authors.forename: Uta
authors.initials: U
authors.lastname: Meyding-Lamade
authors.affiliation: Department of Neurology, Krankenhaus Nordwest, Frankfurt, Germany; The Brunei Neuroscience Stroke and Rehabilitation Centre, Brunei Darussalam.
authors.forename: Eva Maria
authors.initials: EM
authors.lastname: Craemer
authors.affiliation: Pengiran Anak Puteri Rashidah Saadatul Bolkiah Institute of Health Sciences, Universiti Brunei Darussalam, Brunei Darussalam.
authors.forename: Suwarni
authors.initials: S
authors.lastname: Diah
authors.affiliation: Pengiran Anak Puteri Rashidah Saadatul Bolkiah Institute of Health Sciences, Universiti Brunei Darussalam, Brunei Darussalam; Department of Primary Care and Public Health, Faculty of Public Health, Imperial College London, United Kingdom.
authors.forename: Ani Afiqah
authors.initials: AA
authors.lastname: Tuah
authors.affiliation: Pengiran Anak Puteri Rashidah Saadatul Bolkiah Institute of Health Sciences, Universiti Brunei Darussalam, Brunei Darussalam.
authors.forename: Siti Hanna
authors.initials: SH
authors.lastname: Muharram
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000378:metabolism; D002460:Cell Line; D042783:Endothelial Cells / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008954:Models, Biological
keywords: Blood–brain barrier; Endothelial cells; Protein expression; Transendothelial integrity