title: Loktanella marina sp. nov., isolated from seawater.
abstract: A Gram-stain-negative, coccoid- or short-rod-shaped and non-motile bacterial strain, designated MDM-7T, was isolated from seawater of the Yellow Sea, South Korea, and was subjected to a polyphasic taxonomic study. Strain MDM-7Tgrew optimally at pH 7.0-8.0, at 30 °C and in the presence of 2-3 % (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain MDM-7Tfell within the clade comprising species of the genus Loktanella, clustering with the type strains of Loktanella vestfoldensisand Loktanella agnita, with which it exhibited 96.1 and 95.6 % similarity, respectively. 16S rRNA gene sequence similarity values between strain MDM-7Tand the type strains of other Loktanella species were 93.1-95.0 %. The DNA G+C content of strain MDM-7Twas 62.6 mol%. Strain MDM-7Tcontained Q-10 as the predominant ubiquinone and summed feature 8 (C18 : 1ω7c and/ or C18 : 1ω6c) as the major fatty acid. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and an unidentified aminolipid. Differential phenotypic properties, together with phylogenetic distinctiveness, demonstrated that strain MDM-7Tis distinguishable from recognized Loktanella species. On the basis of the data presented, strain MDM-7Tis considered to represent a novel species of the genus Loktanella, for which the name Loktanella marina sp. nov. is proposed. The type strain is MDM-7T(=KCTC 42722T=CECT 8899T).
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.|University of Science and Technology (UST), 113 Gwahak-ro, Yuseong-gu, Daejeon, South Korea.
authors.forename: Yong-Taek
authors.initials: YT
authors.lastname: Jung
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, South Korea.
authors.forename: Jung-Sook
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2
keywords: Alphaproteobacteria; Loktanella marina sp. nov.; Yellow Sea; seawater