title: Proteomic differences between native and tissue-engineered tendon and ligament.
abstract: Tendons and ligaments (T/Ls) play key roles in the musculoskeletal system, but they are susceptible to traumatic or age-related rupture, leading to severe morbidity as well as increased susceptibility to degenerative joint diseases such as osteoarthritis. Tissue engineering represents an attractive therapeutic approach to treating T/L injury but it is hampered by our poor understanding of the defining characteristics of the two tissues. The present study aimed to determine differences in the proteomic profile between native T/Ls and tissue engineered (TE) T/L constructs. The canine long digital extensor tendon and anterior cruciate ligament were analyzed along with 3D TE fibrin-based constructs created from their cells. Native tendon and ligament differed in their content of key structural proteins, with the ligament being more abundant in fibrocartilaginous proteins. 3D T/L TE constructs contained less extracellular matrix (ECM) proteins and had a greater proportion of cellular-associated proteins than native tissue, corresponding to their low collagen and high DNA content. Constructs were able to recapitulate native T/L tissue characteristics particularly with regard to ECM proteins. However, 3D T/L TE constructs had similar ECM and cellular protein compositions indicating that cell source may not be an important factor for T/L tissue engineering.
authors.affiliation: Department of Musculoskeletal Biology, Institute of Ageing and Chronic Disease, University of Liverpool, Leahurst Campus, Neston, UK.
authors.forename: Yalda A
authors.initials: YA
authors.lastname: Kharaz
authors.affiliation: Department of Musculoskeletal Biology, Institute of Ageing and Chronic Disease, University of Liverpool, Leahurst Campus, Neston, UK.
authors.forename: Simon R
authors.initials: SR
authors.lastname: Tew
authors.affiliation: Department of Musculoskeletal Biology, Institute of Ageing and Chronic Disease, University of Liverpool, Leahurst Campus, Neston, UK.|The MRC-Arthritis Research UK Centre for Integrated Research into Musculoskeletal Ageing (CIMA), Liverpool, UK.
authors.forename: Mandy
authors.initials: M
authors.lastname: Peffers
authors.affiliation: Department of Musculoskeletal Biology, Institute of Ageing and Chronic Disease, University of Liverpool, Leahurst Campus, Neston, UK.|The MRC-Arthritis Research UK Centre for Integrated Research into Musculoskeletal Ageing (CIMA), Liverpool, UK.
authors.forename: Elizabeth G
authors.initials: EG
authors.lastname: Canty-Laird
authors.affiliation: Department of Musculoskeletal Biology, Institute of Ageing and Chronic Disease, University of Liverpool, Leahurst Campus, Neston, UK.
authors.forename: Eithne
authors.initials: E
authors.lastname: Comerford
mesh_terms: D000818:Animals; D016118:Anterior Cruciate Ligament / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D004285:Dogs; D005109:Extracellular Matrix / Q000378:metabolism; D017847:Patellar Ligament / Q000166:cytology / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D046509:Tissue Culture Techniques; D023822:Tissue Engineering
chemical_list: D020543:Proteome
keywords: 3D tissue engineered ligament construct; 3D tissue engineered tendon constructs; Ligament; Proteomics; Technology; Tendon