title: Novel E. coli ST5123 Containing blaNDM-1Carried by IncF Plasmid Isolated from a Pediatric Patient in Serbia.
abstract: New Delhi metallo-β-lactamase (NDM) is a serious challenge to the treatment of infections and public health. Serbia has been designated as an endemic region for isolates carrying the blaNDM-1gene, as well as one of several commonly proposed countries of origin. This is the first report of NDM-1-positive Escherichia coli from Serbia. A carbapenem-resistant clinical isolate of E. coli strain IMD989, isolated from the blood culture of a pediatric patient with leukemia, was subjected to antimicrobial susceptibility tests, molecular typing, and conjugation experiments. The strain exhibited resistance to meropenem and was classified as a novel sequence type, ST5123, belonging to E. coli phylogenetic group A. ST5123 showed similarity to veterinary isolates ST93 and ST3977. The blaNDM-1gene was detected by polymerase chain reaction (PCR) and sequencing. Cloning and sequencing of genomic clones confirmed that strain IMD989 produces an NDM-1 variant. Conjugation experiments, pulsed-field gel electrophoresis, and Southern blot hybridization revealed that blaNDM-1was located in IMD989 on a transmissible 80 kb plasmid, designated as pIMD989. PCR analysis confirmed that pIMD989 belongs to the IncF plasmid family. Propagation of IMD989 and selected transconjugants carrying pIMD989 over 14 days in solid media with and without antibiotic selection showed that pIMD989 is a stable plasmid.
authors.affiliation: 1 Institute of Molecular Genetics and Genetic Engineering, University of Belgrade , Belgrade, Serbia .
authors.forename: Katarina
authors.initials: K
authors.lastname: Novovic
authors.affiliation: 2 Institute for Mother and Child Health Care "Dr. Vukan Čupić ," Belgrade, Serbia .
authors.forename: Zorica
authors.initials: Z
authors.lastname: Vasiljevic
authors.affiliation: 2 Institute for Mother and Child Health Care "Dr. Vukan Čupić ," Belgrade, Serbia .|3 Belgrade Medical School, University of Belgrade, Belgrade, Serbia .
authors.forename: Milos
authors.initials: M
authors.lastname: Kuzmanovic
authors.affiliation: 1 Institute of Molecular Genetics and Genetic Engineering, University of Belgrade , Belgrade, Serbia .|4 Faculty of Biology, University of Belgrade , Belgrade, Serbia .
authors.forename: Jelena
authors.initials: J
authors.lastname: Lozo
authors.affiliation: 1 Institute of Molecular Genetics and Genetic Engineering, University of Belgrade , Belgrade, Serbia .
authors.forename: Jelena
authors.initials: J
authors.lastname: Begovic
authors.affiliation: 1 Institute of Molecular Genetics and Genetic Engineering, University of Belgrade , Belgrade, Serbia .
authors.forename: Milan
authors.initials: M
authors.lastname: Kojic
authors.affiliation: 1 Institute of Molecular Genetics and Genetic Engineering, University of Belgrade , Belgrade, Serbia .|4 Faculty of Biology, University of Belgrade , Belgrade, Serbia .
authors.forename: Branko
authors.initials: B
authors.lastname: Jovcic
mesh_terms: D000293:Adolescent; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000150:complications / Q000188:drug therapy / Q000382:microbiology; D015870:Gene Expression; D006801:Humans; D007938:Leukemia / Q000150:complications / Q000188:drug therapy / Q000382:microbiology; D008297:Male; D000077731:Meropenem; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism*; D013845:Thienamycins / Q000494:pharmacology; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D013845:Thienamycins; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1; D000077731:Meropenem
keywords: E. coli ST5123; blaNDM-1; dissemination; pediatric