title: Flexible Method for Analysis of Lidocaine and Its Metabolite in Biological Fluids.
abstract: A robust liquid chromatography-tandem mass spectrometry (LC-MS-MS) method has been developed and validated for the determination of lidocaine (LIDO) and its metabolite (monoethylglycinexylidide (MEGX)) in serum. One hundred microliters of bovine serum were spiked with LIDO-d10 as internal standard and deproteinized with acetonitrile prior to solid-phase extraction purification using strong cation exchange cartridge. The chromatographic separation was achieved on a BetaBasic-18 column with a mobile phase consisting of aqueous 0.1% formic acid and 0.1% formic acid in acetonitrile. The instrumental linearity was verified from 0.4 to 1,000 ng/mL obtaining determination coefficients (r(2)) of >0.99. The limit of quantification (LOQ) was set at 1 ng/mL for both LIDO and MEGX. The coefficients of variation for within- and between-batch imprecision, including LOQ, were ≤10% and the percentage of inaccuracy was <15%. The absolute recoveries were >75% for both analytes. Experiments demonstrated the method applicability to sera of different animal species and also to plasma, urine and milk matrices.
authors.affiliation: Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Via G. Salvemini, 1-06126 Perugia, Italy.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Saluti
authors.affiliation: Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Via G. Salvemini, 1-06126 Perugia, Italy.
authors.forename: Danilo
authors.initials: D
authors.lastname: Giusepponi
authors.affiliation: Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Via G. Salvemini, 1-06126 Perugia, Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Moretti
authors.affiliation: Department of Veterinary Medicine, University of Perugia, Via S. Costanzo, 4-06126 Perugia, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Di Salvo
authors.affiliation: Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Via G. Salvemini, 1-06126 Perugia, Italy r.galarini@izsum.it.
authors.forename: Roberta
authors.initials: R
authors.lastname: Galarini
mesh_terms: D000097:Acetonitriles; D000818:Animals; D001711:Biotransformation; D002415:Cats; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods / Q000592:standards*; D004285:Dogs; D005561:Formates; D006736:Horses; D006801:Humans; D008012:Lidocaine / Q000031:analogs & derivatives* / Q000097:blood* / Q000652:urine; D057230:Limit of Detection; D008892:Milk / Q000737:chemistry; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods; D012997:Solvents; D053719:Tandem Mass Spectrometry / Q000379:methods / Q000592:standards*
chemical_list: D000097:Acetonitriles; D005561:Formates; D012997:Solvents; C030544:formic acid; D008012:Lidocaine; C001173:monoethylglycinexylidide; C032159:acetonitrile