title: Preparation of magnetic ODS-PAN thin-films for microextraction of quetiapine and clozapine in plasma and urine samples followed by HPLC-UV detection.
abstract: In this study, conventional thin-film microextraction (TFME) was endowed with magnetic by introducing superparamagnetic SiO2@Fe3O4 nanoparticles in thin-films. Novel magnetic octadecylsilane (ODS)-polyacrylonitrile (PAN) thin-films were prepared by spraying, and used for the microextraction of quetiapine and clozapine in plasma and urine samples, followed by the detection of HPLC-UV. The influencing factors on the extraction efficiency of magnetic ODS-PAN TFME, including pH, extraction time, desorption solvent, desorption time, and ion strength were investigated systematically. Under the optimal conditions, both analytes showed good linearity over ranges of 0.070-9.000μgmL(-1) and 0.012-9.000μgmL(-1) in plasma and urine samples, respectively, with correlation coefficients (R(2)) above 0.9990. Limits of detection (LODs) for quetiapine in plasma and urine samples were 0.013 and 0.003μgmL(-1), respectively. LODs for clozapine in plasma and urine samples were 0.015 and 0.003μgmL(-1), respectively. The relative standard deviations (RSDs) for quetiapine and clozapine were less than 9.23%. After the validation, the protocol was successfully applied for the determination of quetiapine and clozapine in patients' plasma and urine samples with satisfactory recoveries between 99-110%. The proposed magnetic ODS-PAN TFME was very simple, fast and easy to handle. It showed high potential as a powerful pretreatment technology for routine therapeutic drug monitoring (TDM) in plasma and urine samples.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zou
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, PR China.
authors.forename: Pei-Shan
authors.initials: PS
authors.lastname: Cai
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, PR China. Electronic address: cmxiong2002@hust.edu.cn.
authors.forename: Chao-Mei
authors.initials: CM
authors.lastname: Xiong
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, PR China.
authors.forename: Jin-Lan
authors.initials: JL
authors.lastname: Ruan
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003024:Clozapine / Q000097:blood* / Q000652:urine*; D006801:Humans; D057230:Limit of Detection; D008280:Magnetics; D008855:Microscopy, Electron, Scanning; D000069348:Quetiapine Fumarate / Q000097:blood* / Q000652:urine*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D000069348:Quetiapine Fumarate; D003024:Clozapine
keywords: Clozapine; HPLC-UV; Magnetic octadecylsilane-polyacrylonitrile thin-film microextraction (ODS-PAN TFME); Plasma; Quetiapine; Therapeutic drug monitoring (TDM); Urine