title: Structural and Thermodynamic Characterization of Protein-Ligand Interactions Formed between Lipoprotein-Associated Phospholipase A2 and Inhibitors.
abstract: Lipoprotein-associated phospholipase A2 (Lp-PLA2) represents a promising therapeutic target for atherosclerosis and Alzheimer's disease. Here we reported the first crystal structures of Lp-PLA2 bound with reversible inhibitors and the thermodynamic characterization of complexes. High rigidity of Lp-PLA2 structure and similar binding modes of inhibitors with completely different scaffolds are revealed. It not only provides the molecular basis for inhibitory activity but also sheds light on the essential features of Lp-PLA2 recognition with reversible inhibitors.
authors.affiliation: School of Life Science and Technology, ShanghaiTech University , Shanghai 200031, China.
authors.forename: Qiufeng
authors.initials: Q
authors.lastname: Liu
authors.forename: Xinde
authors.initials: X
authors.lastname: Chen
authors.forename: Wuyan
authors.initials: W
authors.lastname: Chen
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Yuan
authors.forename: Haixia
authors.initials: H
authors.lastname: Su
authors.forename: Jianhua
authors.initials: J
authors.lastname: Shen
authors.forename: Yechun
authors.initials: Y
authors.lastname: Xu
mesh_terms: D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015195:Drug Design; D006801:Humans; D008024:Ligands; D008958:Models, Molecular; D015394:Molecular Structure; D064801:Phospholipase A2 Inhibitors / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013816:Thermodynamics
chemical_list: D008024:Ligands; D064801:Phospholipase A2 Inhibitors; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; C526684:PLA2G7 protein, human