title: Relationship between leptin concentration and body fat with peripheral blood mononuclear cells cytokines among obese and overweight adults.
abstract: BACKGROUND
  Overweight and obesity has been suggested to be well correlated with altered levels of pro-inflammatory cytokines.
  
  
  OBJECTIVE
  The purpose of this study is to assess the relationship of body fat mass (BFM), body fat percentage (BFP) and leptin levels with peripheral blood mononuclear cells (PBMCs) cytokines among obese and overweight adults.
  
  
  METHODS
  Eighty-two overweight and obese individuals were divided into two BMI-category groups (BMI <30 and BMI ≥30 kg/m2) in this study. Balanced blocked randomization was used based on their sex and BMI ranges. Fasting blood samples, PBMCs cytokines, leptin and anthropometric indices were measured and PBMCs were cultured.
  
  
  RESULTS
  Mean of leptin concentrations were 23.14 ± 4.07 and 28.25 ± 4.35 pg/ml among individuals with BMI <30 and BMI ≥30 kg/m2, respectively. The mean values of anthropometric measurements (all P < 0.001), the concentrations of TNF-α (P = 0.028) and IFN-γ (P = 0.029) were significantly higher among obese individuals. BFP had a significant positive correlation with leptin (P < 0.001, r = 0.445) and TGF-β (P = 0.03, r = 0.243). BFM has significant positive correlation with leptin (P < 0.001, r = 0.521). Leptin had a positive significant correlation with IFN-γ (p = 0.03, r = 0.251).
  
  
  CONCLUSIONS
  Regarding these results, we proved that BFP, BFM and leptin levels have significant correlations with some PBMC cytokines. Focusing on such strategies may lead to promises for alleviating obesity and its co-morbidities.
authors.affiliation: School of Public Health, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Zarrati
authors.affiliation: Immunology Departments, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: E
authors.initials: E
authors.lastname: Salehi
authors.affiliation: Nutrition and Food Security Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.|Department of Nutrition, Faculty of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: E
authors.initials: E
authors.lastname: Razmpoosh
authors.affiliation: Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: R S
authors.initials: RS
authors.lastname: Shoormasti
authors.affiliation: Department of Nutrition, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: M J
authors.initials: MJ
authors.lastname: Hosseinzadeh-Attar
authors.affiliation: School of Public Health, Iran University of Medical Sciences, Tehran, Iran. farzadshidfar@yahoo.com.|Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran. farzadshidfar@yahoo.com.
authors.forename: F
authors.initials: F
authors.lastname: Shidfar
mesh_terms: D000273:Adipose Tissue; D000328:Adult; D015992:Body Mass Index; D016207:Cytokines / Q000097:blood; D005260:Female; D006801:Humans; D020738:Leptin / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood*; D050177:Overweight / Q000097:blood*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D016207:Cytokines; D020738:Leptin; D014409:Tumor Necrosis Factor-alpha
keywords: Body fat percent; Cytokine; Leptin; Obesity; PBMCs