title: Photomodulation of the osteoclastogenic potential of oral squamous carcinoma cells.
abstract: The treatment for oral cancer usually involves surgical excision followed by chemotherapy and/or radiotherapy. The combination of these therapies generally promotes a serious inflammation of the mucosa of the digestive tract, denominated mucositis, which compromises continuity of treatment. Photobiomodulation (PBM) therapy has been used successfully to reduce the oral mucositis, however there is still some controversy regarding the effects of this therapy on unintentionally irradiated tumor cells that may remain after cancer treatment. The aim of this study was to analyze the effect of PBM therapy (using parameters for mucositis) on the modulation of osteoclastogenic potential of a cell line derived from human lingual squamous cell carcinoma (SCC9). Previously irradiated SCC9 cells were co-cultured with human osteoclast precursors. Co-cultures performed with non-irradiated SCC9 cells served as control. After 7, 14 and 21 days the co-cultures were evaluated for the tartrate-resistant acid phosphatase (TRAP) activity, an osteoclastogenic marker. Additionally, the monocultures of SCC9 cells (non-irradiated and irradiated) were analyzed for cell viability/proliferation and for the expression of IL-11 and PTHrP. The irradiation of SCC9 cells with PBM with an energy density of 4 J/cm2decreased the pro-osteoclastogenic potential of those cells. This may represent a potential useful side effect of PBM therapy. PBM (using recommended parameters for mucositis treatment) decreases the osteoclastogenic potential of oral squamous carcinoma cells.
authors.affiliation: Biophotonics Applied to Health Sciences Postgraduate Program, Nove de Julho University - UNINOVE, 235/249 Vergueiro Street, 01504-001, São Paulo, Brazil.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Dias Schalch
authors.affiliation: Biophotonics Applied to Health Sciences Postgraduate Program, Nove de Julho University - UNINOVE, 235/249 Vergueiro Street, 01504-001, São Paulo, Brazil.
authors.forename: Kristianne
authors.initials: K
authors.lastname: Porta Santos Fernandes
authors.affiliation: Laboratory for Bone Metabolism and Regeneration, Faculdade de Medicina Dentária, Universidade do Porto, Portugal, Dr. Manuel Pereira da Silva Street, 4200-393, Porto, Portugal.
authors.forename: João
authors.initials: J
authors.lastname: Costa-Rodrigues
authors.affiliation: Laboratory for Bone Metabolism and Regeneration, Faculdade de Medicina Dentária, Universidade do Porto, Portugal, Dr. Manuel Pereira da Silva Street, 4200-393, Porto, Portugal.
authors.forename: Mônica
authors.initials: M
authors.lastname: Pereira Garcia
authors.affiliation: Biophotonics Applied to Health Sciences Postgraduate Program, Nove de Julho University - UNINOVE, 235/249 Vergueiro Street, 01504-001, São Paulo, Brazil.
authors.forename: Raquel
authors.initials: R
authors.lastname: Agnelli Mesquita-Ferrari
authors.affiliation: Biophotonics Applied to Health Sciences Postgraduate Program, Nove de Julho University - UNINOVE, 235/249 Vergueiro Street, 01504-001, São Paulo, Brazil.
authors.forename: Sandra
authors.initials: S
authors.lastname: Kalil Bussadori
authors.affiliation: Laboratory for Bone Metabolism and Regeneration, Faculdade de Medicina Dentária, Universidade do Porto, Portugal, Dr. Manuel Pereira da Silva Street, 4200-393, Porto, Portugal.
authors.forename: Maria Helena
authors.initials: MH
authors.lastname: Fernandes
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000532:radiotherapy*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004847:Epithelial Cells / Q000528:radiation effects*; D006801:Humans; D009062:Mouth Neoplasms / Q000532:radiotherapy*; D010012:Osteogenesis; D010789:Phototherapy
keywords: low-level laser therapy; mouth neoplasms; mucositis; osteoclastogenesis; photobiomodulation