title: Variation of the microbial community in thermophilic anaerobic digestion of pig manure mixed with different ratios of rice straw.
abstract: The effect of pig manure mixed with rice straw on methane yield and the microbial community involved in a thermophilic (55°C) anaerobic digestion process was investigated. Three substrates composed of mixed pig manure and rice straw at different ratios (95:5; 78:22 and 65:35 w/w, which resulted in C/N ratios of 10:1, 20:1 and 30:1) were used for the experiment. The substrate type had a major influence on the total bacterial community, while the methanogens were less affected. The members of the class Clostridia (phylum Firmicutes) were predominant regardless of mixture ratio (C/N ratio), but at species level there was a major difference between the low and high C/N ratio samples. The hydrogenotrophic methanogenic genus of Methanothermobacter was predominant in all samples but higher C/N ratio sequences affiliated to the genus Methanosarcina were also detected. The appearance of Methanosarcina sp. is most likely due to the less inhibition of ammonia during the anaerobic digestion.
authors.affiliation: Eco-environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, No. 1000 Jinqi Road, Shanghai 201403, China. Electronic address: zhous@outlook.com.
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Environmental Microbiology, UFZ - Helmholtz Centre for Environmental Research, Permoserstraße 15, 04318 Leipzig, Germany.
authors.forename: Marcell
authors.initials: M
authors.lastname: Nikolausz
authors.affiliation: Eco-environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, No. 1000 Jinqi Road, Shanghai 201403, China.
authors.forename: Jining
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors.forename: Shohei
authors.initials: S
authors.lastname: Riya
authors.affiliation: Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Terada
authors.affiliation: Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Hosomi
mesh_terms: D000641:Ammonia / Q000378:metabolism; D000693:Anaerobiosis; D000818:Animals; D019149:Bioreactors / Q000382:microbiology; D002244:Carbon / Q000378:metabolism; D000068536:Bacillota / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008372:Manure / Q000032:analysis* / Q000382:microbiology*; D008697:Methane / Q000096:biosynthesis* / Q000378:metabolism; D017013:Methanobacteriaceae / Q000378:metabolism; D017020:Methanosarcina / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D012275:Oryza / Q000737:chemistry*; D013552:Swine; D013696:Temperature
chemical_list: D008372:Manure; D002244:Carbon; D000641:Ammonia; D006859:Hydrogen; D009584:Nitrogen; D008697:Methane
keywords: Ammonia; Bacterial community; Hydrogenotrophic methanogen; Terminal restriction fragment-length polymorphisms; Thermophilic