title: Production of 1,3-propanediol by Clostridium beijerinckii DSM 791 from crude glycerol and corn steep liquor: Process optimization and metabolic engineering.
abstract: 1,3-Propanediol (1,3-PDO) production from crude glycerol, a byproduct from biodiesel manufacturing, by Clostridium beijerinckii DSM 791 was studied with corn steep liquor as an inexpensive nitrogen source replacing yeast extract in the fermentation medium. A stable, long-term 1,3-PDO production from glycerol was demonstrated with cells immobilized in a fibrous bed bioreactor operated in a repeated batch mode, which partially circumvented the 1,3-PDO inhibition problem. The strain was then engineered to overexpress Escherichia coli gldA encoding glycerol dehydrogenase (GDH) and dhaKLM encoding dihydroxyacetone kinase (DHAK), which increased 1,3-PDO productivity by 26.8-37.5% compared to the wild type, because of greatly increased specific growth rate (0.25-0.40h(-1) vs. 0.13-0.20h(-1) for the wild type). The engineered strain gave a high 1,3-PDO titer (26.1g/L), yield (0.55g/g) and productivity (0.99g/L·h) in fed-batch fermentation. Overexpressing GDH and DHAK was thus effective in increasing 1,3-PDO production from glycerol.
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Avenue, Columbus, OH 43210, USA; School of Chemistry, Department of Biochemical Engineering, Federal University of Rio de Janeiro, Av. Horácio Macedo 2030, Bloco E., Rio de Janeiro, RJ 21949-900, Brazil.
authors.forename: Daiana
authors.initials: D
authors.lastname: Wischral
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Avenue, Columbus, OH 43210, USA.
authors.forename: Jianzhi
authors.initials: J
authors.lastname: Zhang
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Avenue, Columbus, OH 43210, USA.
authors.forename: Chi
authors.initials: C
authors.lastname: Cheng
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Avenue, Columbus, OH 43210, USA.
authors.forename: Meng
authors.initials: M
authors.lastname: Lin
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Avenue, Columbus, OH 43210, USA.
authors.forename: Lucas Monteiro Galotti
authors.initials: LMG
authors.lastname: De Souza
authors.affiliation: School of Chemistry, Department of Chemical Engineering, Federal University of Rio de Janeiro, Av. Horácio Macedo 2030, Bloco E., Rio de Janeiro, RJ 21949-900, Brazil.
authors.forename: Fernando L Pellegrini
authors.initials: FLP
authors.lastname: Pessoa
authors.affiliation: School of Chemistry, Department of Biochemical Engineering, Federal University of Rio de Janeiro, Av. Horácio Macedo 2030, Bloco E., Rio de Janeiro, RJ 21949-900, Brazil.
authors.forename: Nei
authors.initials: N
authors.lastname: Pereira
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Avenue, Columbus, OH 43210, USA. Electronic address: yang.15@osu.edu.
authors.forename: Shang-Tian
authors.initials: ST
authors.lastname: Yang
mesh_terms: D000085:Acetates / Q000378:metabolism; D061249:Batch Cell Culture Techniques; D019149:Bioreactors / Q000382:microbiology; D002087:Butyrates / Q000378:metabolism; D046970:Clostridium beijerinckii / Q000378:metabolism*; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D060847:Metabolic Engineering / Q000379:methods*; D053858:Metabolic Networks and Pathways; D010957:Plasmids / Q000378:metabolism; D011409:Propylene Glycols / Q000378:metabolism*; D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism; D014865:Waste Disposal, Fluid; D003313:Zea mays / Q000737:chemistry*
chemical_list: D000085:Acetates; D002087:Butyrates; D011409:Propylene Glycols; C041787:1,3-propanediol; D013401:Sugar Alcohol Dehydrogenases; C020672:glycerol dehydrogenase; D005990:Glycerol
keywords: 1,3-Propanediol; Clostridium beijerinckii DSM 791; Dihydroxyacetone kinase; Glycerol; Glycerol dehydrogenase