title: Role of DAF-21protein in Caenorhabditis elegans immunity against Proteus mirabilis infection.
abstract: Caenorhabditis elegans is emerging as one of the handy model for proteome related studies due to its simplest system biology. The present study, deals with changes in protein expression in C. elegans infected with Proteus mirabilis. Proteins were separated using two-dimensional differential gel electrophoresis (2D-DIGE) and identified using MALDI-TOF. Twelve distinctly regulated proteins identified in the infected worms, included heat shock proteins involved stress pathway (HSP-1 and HSP-6), proteins involved in immune response pathway (DAF-21), enzymes involved in normal cellular process (Eukaryotic translation Elongation Factor, actin family member, S-adenosyl homocysteine hydrolase ortholog, glutamate dehydrogenase and Vacuolar H ATPase family member) and few least characterized proteins (H28O16.1 and H08J11.2). The regulation of selected players at the transcriptional level during Proteus mirabilis infection was analyzed using qPCR. Physiological experiments revealed the ability of P. mirabilis to kill daf-21 mutant C. elegans significantly compared with the wild type. This is the first report studying proteome changes in C. elegans and exploring the involvement of MAP Kinase pathway during P. mirabilis infection.
  
  
  
  This is the first report studying proteome changes in C. elegans during P. mirabilis infection. The present study explores the role and contribution of MAP Kinase pathway and its regulator protein DAF-21 involvement in the immunity against opportunistic pathogen P. mirabilis infection. Manipulation of this DAF-21 protein in host, may pave the way for new drug development or disease control strategy during opportunistic pathogen infections.
authors.affiliation: Department of Biotechnology, Alagappa University, Karaikudi 630004, India.
authors.forename: Gnanasekaran
authors.initials: G
authors.lastname: JebaMercy
authors.affiliation: Department of Biotechnology, Alagappa University, Karaikudi 630004, India.
authors.forename: Sellegounder
authors.initials: S
authors.lastname: Durai
authors.affiliation: Department of Biotechnology, Alagappa University, Karaikudi 630004, India.
authors.forename: Udayakumar
authors.initials: U
authors.lastname: Prithika
authors.affiliation: Department of Biotechnology, Alagappa University, Karaikudi 630004, India.
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Marudhupandiyan
authors.affiliation: Department of Biochemistry, University of Delhi South Campus, New Delhi 110021, India.
authors.forename: Pushpanjali
authors.initials: P
authors.lastname: Dasauni
authors.affiliation: Department of Biochemistry, University of Delhi South Campus, New Delhi 110021, India.
authors.forename: Suman
authors.initials: S
authors.lastname: Kundu
authors.affiliation: Department of Biotechnology, Alagappa University, Karaikudi 630004, India. Electronic address: bsuryar@yahoo.com.
authors.forename: Krishnaswamy
authors.initials: K
authors.lastname: Balamurugan
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000276:immunology / Q000382:microbiology*; D029742:Caenorhabditis elegans Proteins / Q000276:immunology*; D020869:Gene Expression Profiling; D018841:HSP90 Heat-Shock Proteins / Q000276:immunology*; D006360:Heat-Shock Proteins / Q000032:analysis; D020935:MAP Kinase Signaling System / Q000276:immunology; D020543:Proteome / Q000032:analysis / Q000276:immunology; D040901:Proteomics / Q000379:methods*; D011512:Proteus Infections; D011513:Proteus mirabilis / Q000472:pathogenicity*
chemical_list: D029742:Caenorhabditis elegans Proteins; C510188:DAF-21 protein, C elegans; D018841:HSP90 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D020543:Proteome
keywords: 2D-DIGE; C. elegans; DAF-21; Host-pathogen interaction; MALDI-TOF; P. mirabilis