title: Effect of the addition of flavan-3-ols on the HPLC-DAD salivary-protein profile.
abstract: The interaction between monomeric flavan-3-ols and salivary proteins has been studied using HPLC-DAD. A chromatographic method has been described and seven protein fractions were collected. The peptides and proteins present in each fraction have been identified using nLC-MS-MS analysis. The interaction between saliva and catechin, epicatechin and gallocatechin has been studied. These compounds interact in a discriminated way with salivary proteins: catechin causes a decrease of some fractions, epicatechin causes the decrease or increase of fractions while gallocatechin seems to cause an increase of two fractions. This variable behavior is explained, for the decrease in the chromatographic area, by the precipitation of salivary proteins and, for the increase of the area, by the formation of soluble complexes and/or for the formation of new peaks.
authors.affiliation: Grupo de Investigación en Polifenoles, Unidad de Nutrición y Bromatología, Facultad de Farmacia, University of Salamanca, Campus Miguel de Unamuno, E 37007 Salamanca, Spain. Electronic address: nataliaquijada@usal.es.
authors.forename: Natalia
authors.initials: N
authors.lastname: Quijada-Morín
authors.affiliation: Grupo de Investigación en Polifenoles, Unidad de Nutrición y Bromatología, Facultad de Farmacia, University of Salamanca, Campus Miguel de Unamuno, E 37007 Salamanca, Spain. Electronic address: carlos.crespo@usal.es.
authors.forename: Carlos
authors.initials: C
authors.lastname: Crespo-Expósito
authors.affiliation: Grupo de Investigación en Polifenoles, Unidad de Nutrición y Bromatología, Facultad de Farmacia, University of Salamanca, Campus Miguel de Unamuno, E 37007 Salamanca, Spain. Electronic address: jcrivas@usal.es.
authors.forename: Julián C
authors.initials: JC
authors.lastname: Rivas-Gonzalo
authors.affiliation: Grupo de Investigación en Polifenoles, Unidad de Nutrición y Bromatología, Facultad de Farmacia, University of Salamanca, Campus Miguel de Unamuno, E 37007 Salamanca, Spain. Electronic address: igarest@usal.es.
authors.forename: Ignacio
authors.initials: I
authors.lastname: García-Estévez
authors.affiliation: Grupo de Investigación en Polifenoles, Unidad de Nutrición y Bromatología, Facultad de Farmacia, University of Salamanca, Campus Miguel de Unamuno, E 37007 Salamanca, Spain. Electronic address: escriban@usal.es.
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Escribano-Bailón
mesh_terms: D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005419:Flavonoids / Q000737:chemistry*; D006801:Humans; D008297:Male; D011485:Protein Binding; D012471:Salivary Proteins and Peptides / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D005419:Flavonoids; D012471:Salivary Proteins and Peptides; C404987:flavan-3-ol; D002392:Catechin
keywords: Astringency; Flavan-3-ol; HPLC-DAD; Salivary protein; Soluble aggregates