title: Influence of Polymer Size on Uptake and Cytotoxicity of Doxorubicin-Loaded DNA-PEG Conjugates.
abstract: Intercalation of drugs into assembled DNA systems offers versatile new mechanisms for controlled drug delivery. However, current systems are becoming increasingly complex, reducing the practicality of large scale production. Here, we demonstrate a more pragmatic approach where a short DNA sequence was modified with poly(ethylene glycol) (PEG) of various lengths at both 5'-termini to provide serum stability and compatibility. The anticancer drug doxorubicin was physically loaded into two designed binding sites on the dsODN. The polymer conjugation improved the stability of the dsODN toward serum nucleases while its doxorubicin binding affinity was unaffected by the presence of the polymers. We examined the effects of polymer size on the dsODN carrier characteristics and studied the resulting DOX@DNA-PEG systems with respect to cytotoxicity, cellular uptake, and localization in A549 and MCF7 cell lines. For the A549 cell line the DOX@DNA-PEG1900 exhibited the best dose response of the conjugates while DOX@DNA-PEG550 was the least potent. In MCF-7, a more doxorubicin sensitive cell line, all conjugates exhibited similar dose response to that of the free drug. Confocal microscopy analysis of doxorubicin localization shows that conjugates successfully deliver doxorubicin to the cell nucleus and also the lysosome. These data provide a valuable insight into the complexities of designing an oligonucleotide based drug delivery system and highlight some practical issues that need to be considered when doing so.
authors.affiliation: School of Pharmacy, University of Nottingham , University Park, Nottingham, United Kingdom NG7 2RD.
authors.forename: Laura
authors.initials: L
authors.lastname: Purdie
authors.affiliation: School of Pharmacy, University of Nottingham , University Park, Nottingham, United Kingdom NG7 2RD.
authors.forename: Cameron
authors.initials: C
authors.lastname: Alexander
authors.affiliation: School of Pharmacy, University of Nottingham , University Park, Nottingham, United Kingdom NG7 2RD.
authors.forename: Sebastian G
authors.initials: SG
authors.lastname: Spain
authors.affiliation: School of Pharmacy, University of Nottingham , University Park, Nottingham, United Kingdom NG7 2RD.
authors.forename: Johannes P
authors.initials: JP
authors.lastname: Magnusson
mesh_terms: D001692:Biological Transport; D004247:DNA / Q000737:chemistry*; D004317:Doxorubicin / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry*; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D061986:MCF-7 Cells; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D011092:Polyethylene Glycols; D004317:Doxorubicin; D004247:DNA