title: Generation of Newcastle Disease Virus (NDV) Recombinants Expressing the Infectious Laryngotracheitis Virus (ILTV) Glycoprotein gB or gD as Dual Vaccines.
abstract: Infectious laryngotracheitis (ILT) is a highly contagious acute respiratory disease of chickens caused by infection with infectious laryngotracheitis virus (ILTV), a member of the family Herpesviridae. The current commercial ILT vaccines are either unsafe or ineffective. Therefore, there is a pressing need to develop safer and more efficacious vaccines. Newcastle disease (ND), caused by infection with Newcastle disease virus (NDV), a member of the family Paramyxoviridae, is one of the most serious infectious diseases of poultry. The NDV LaSota strain, a naturally occurring low-virulence NDV strain, has been routinely used as a live vaccine throughout the world. This chapter describes the generation of Newcastle disease virus (NDV) LaSota vaccine strain-based recombinant viruses expressing glycoprotein B (gB) or glycoprotein D (gD) of ILTV as dual vaccines against ND and ILT using reverse genetics technology.
authors.affiliation: United States Department of Agriculture, US National Poultry Research Center, Agricultural Research Service, 934 College Station Road, Athens, GA, 30605, USA.|Beijing Centre for Disease Control and Prevention, Beijing, 100013, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: United States Department of Agriculture, US National Poultry Research Center, Agricultural Research Service, 934 College Station Road, Athens, GA, 30605, USA.
authors.forename: Stephen
authors.initials: S
authors.lastname: Spatz
authors.affiliation: United States Department of Agriculture, US National Poultry Research Center, Agricultural Research Service, 934 College Station Road, Athens, GA, 30605, USA.
authors.forename: Laszlo
authors.initials: L
authors.lastname: Zsak
authors.affiliation: United States Department of Agriculture, US National Poultry Research Center, Agricultural Research Service, 934 College Station Road, Athens, GA, 30605, USA. qingzhong.yu@ars.usda.gov.
authors.forename: Qingzhong
authors.initials: Q
authors.lastname: Yu
mesh_terms: D000818:Animals; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D006386:Hemagglutination Tests; D001352:Herpesvirus 1, Gallid / Q000235:genetics / Q000276:immunology*; D009522:Newcastle disease virus / Q000235:genetics / Q000276:immunology*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D059386:Reverse Genetics / Q000379:methods*; D014170:Transformation, Genetic; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics* / Q000276:immunology*
chemical_list: D018076:DNA, Complementary; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C067381:GB protein, Infectious laryngotracheitis virus
keywords: Glycoproteins B (gB) and D (gD); Infectious laryngotracheitis virus (ILTV); LaSota vaccine strain; Newcastle disease virus (NDV); Recombinant virus; Reverse genetics