title: Combination of Sonodynamic and Photodynamic Therapy against Cancer Would Be Effective through Using a Regulated Size of Nanoparticles.
abstract: Nanoparticles have been used for many functional materials in nano-sciences and photo-catalyzing surface chemistry. The titanium oxide nanoparticles will be useful for the treatment of tumor by laser and/or ultrasound as the sensitizers in nano-medicine. We have studied the combination therapy of photo- and sono-dynamic therapies in an animal tumor model. Oral-administration of two sensitizers titanium oxide, 0.2%-TiO2nanoparticles for sono-dynamic and 1 mM 5-aminolevulinic acid for photodynamic therapies have resulted in the best combination therapeutic effects for the cancer treatment. Our light microscopic and Raman spectroscopic studies revealed that the titanium nanoparticles were distributed inside the blood vessel of the cancer tissue (1-3 μm sizes). Among these nanoparticles with a broad size distribution, only particular-sized particles could penetrate through the blood vessel of the cancer tissue, while other particles may only exhibit the side effects in the model mouse. Therefore, it may be necessary to separate the optimum size particles. For this purpose we have separated TiO2nanoparticles by countercurrent chromatography with a flat coiled column (1.6 mm ID) immersed in an ultrasonic bath (42 KHz). Separation was performed with a two-phase solvent system composed of 1-butanol-acetic acid-water at a volume ratio of 4:1:5 at a flow rate of 0.1 ml/min. Countercurrent chromatographic separation yielded fractions containing particle aggregates at 31 and 4400 nm in diameter.
authors.affiliation: Department of Tumor Pathology, Faculty of Medical Sciences, University of Fukui, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Miyoshi
authors.affiliation: Department of Tumor Pathology, Faculty of Medical Sciences, University of Fukui, Japan; Department of Pharmacy, Jahangirnagar University, Bangladesh.
authors.forename: S K
authors.initials: SK
authors.lastname: Kundu
authors.affiliation: National Institute of Advanced Industrial Science and Technology (NAIST), Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tuziuti
authors.affiliation: National Institute of Advanced Industrial Science and Technology (NAIST), Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yasui
authors.affiliation: Department of Forensic Medicine, Faculty of Medicine, University of Fukui, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Shimada
authors.affiliation: Laboratory of Bioseparation Technology, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
keywords: 5-aminolevulinic Acid (5-ALA); Countercurrent Chromatography (CCC); Photo-dynamic Therapy; Raman Spectrum Microscope; Sono-dynamic Therapy (SDT); Titanium Dioxide (TiO2) Nanoparticles