title: IL-6 induced proliferation and cytotoxic activity of CD8(+) T cells is elevated by SUMO2 overexpression.
abstract: T cells play an important role in adaptive immune responses that destroy pathogens or infected cells. Therefore, regulation of T cell activity is important in various diseases, such as autoimmune diseases, hypersensitivity, and cancer. The conjugation of small ubiquitin-related modifier (SUMO) is a post-translational protein modification that regulates activity, stability, and subcellular translocation of target proteins. In this study, CD8(+) T cells overexpressing SUMO2 showed greater proliferation and cytotoxic activity against tumor cells in the presence of IL-6 than wild-type CD8(+) T cells in vitro. These CD8(+) T cell functions were suppressed during treatment with MEK1 or PI3K-specific inhibitors. Therefore, our findings suggest that IL-6-derived signaling pathways, including the MEK1 and PI3K pathways, are upregulated by SUMO2 overexpression. However, transgenic expression of SUMO2 in T cells did not modulate Th1/2 balance. Collectively, our results showed that SUMO2-Tg promotes cytotoxic activity against tumor cells by increasing the proliferation and cytotoxicity of CD8(+) T cells.
authors.affiliation: Laboratory of Host Defense Modulation, College of Pharmacy, Chung-Ang University, 221 Heukseok-dong, Dongjak-gu, Seoul, 156-756, Republic of Korea.
authors.forename: Yun-Jung
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Laboratory of Host Defense Modulation, College of Pharmacy, Chung-Ang University, 221 Heukseok-dong, Dongjak-gu, Seoul, 156-756, Republic of Korea.
authors.forename: Tae Joon
authors.initials: TJ
authors.lastname: Won
authors.affiliation: Laboratory of Host Defense Modulation, College of Pharmacy, Chung-Ang University, 221 Heukseok-dong, Dongjak-gu, Seoul, 156-756, Republic of Korea.
authors.forename: Kyeong Eun
authors.initials: KE
authors.lastname: Hyung
authors.affiliation: Laboratory of Host Defense Modulation, College of Pharmacy, Chung-Ang University, 221 Heukseok-dong, Dongjak-gu, Seoul, 156-756, Republic of Korea.
authors.forename: Ye Won
authors.initials: YW
authors.lastname: Jang
authors.affiliation: Laboratory of Host Defense Modulation, College of Pharmacy, Chung-Ang University, 221 Heukseok-dong, Dongjak-gu, Seoul, 156-756, Republic of Korea.
authors.forename: Soo Jeong
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Laboratory of Immunology, College of Pharmacy, Chung-Ang University, 221 Heukseok-dong, Dongjak-gu, Seoul, 156-756, Republic of Korea.
authors.forename: Do Ik
authors.initials: do I
authors.lastname: Lee
authors.affiliation: Laboratory of Pharmacognosy, College of Pharmacy, Dankook University, Cheonan, 330-714, Republic of Korea.
authors.forename: So-Young
authors.initials: SY
authors.lastname: Park
authors.affiliation: Laboratory of Host Defense Modulation, College of Pharmacy, Chung-Ang University, 221 Heukseok-dong, Dongjak-gu, Seoul, 156-756, Republic of Korea. khwang@cau.ac.kr.
authors.forename: Kwang Woo
authors.initials: KW
authors.lastname: Hwang
mesh_terms: D056704:Adaptive Immunity; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival / Q000276:immunology; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000276:immunology; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood; D015850:Interleukin-6 / Q000276:immunology* / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000276:immunology; D048369:MAP Kinase Kinase 1 / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D025841:Small Ubiquitin-Related Modifier Proteins / Q000235:genetics* / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology / Q000378:metabolism
chemical_list: D015850:Interleukin-6; C515972:SUMO2 protein, mouse; D025841:Small Ubiquitin-Related Modifier Proteins; D007073:Immunoglobulin E; D019869:Phosphatidylinositol 3-Kinases; D048369:MAP Kinase Kinase 1; C482127:Map2k1 protein, mouse
keywords: CD8+ T cells; Cytotoxicity; IL-6; Proliferation; SUMO2