title: Cardioprotective Effects of Genistin in Rat Myocardial Ischemia-Reperfusion Injury Studies by Regulation of P2X7/NF-κB Pathway.
abstract: The present study aimed to assess the effects and mechanisms of genistin in the rat model of myocardial ischemia reperfusion injury. The rat hearts were exposed to the left anterior descending coronary artery (LAD) ligation for 30 min followed by 1 h of reperfusion. In the rat of myocardial ischemia/reperfusion (MI/R), it was found that genistin pretreatment reduced myocardial infarct size, improved the heart rate, and decreased creatine kinase (CK) and lactate dehydrogenase (LDH) levels in coronary flow. This pretreatment also increased catalase (CAT), superoxide dismutase (SOD) activities but decreased glutathione (GSH), malondialdehyde (MDA) levels. Furthermore, we determined that genistin can ameliorate the impaired mitochondrial morphology and oxidation system; interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10), and tumor necrosis factor-α (TNF-α) levels were also recovered. Besides, related-proteins of nuclear factor kappa-B (NF-κB) signal pathway activated by P2X7 were investigated to determine the molecular mechanism of genistin and their expressions were measured by western blot. These results presented here demonstrated that genistin enhanced the protective effect on the rats with myocardial ischemia reperfusion injury. Therefore, the cardioprotective effects of genistin may rely on its antioxidant and anti-inflammatory activities via suppression of P2X7/NF-κB pathways.
authors.affiliation: Department of Clinical Laboratory, Changzhou Children's Hospital, Changzhou, Jiangsu 213001, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Gu
authors.affiliation: Department of Clinical Laboratory, Changzhou Children's Hospital, Changzhou, Jiangsu 213001, China.
authors.forename: Ai-Bin
authors.initials: AB
authors.lastname: Zheng
authors.affiliation: Department of Clinical Laboratory, Changzhou Children's Hospital, Changzhou, Jiangsu 213001, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Jin
authors.affiliation: Department of Clinical Laboratory, Changzhou Children's Hospital, Changzhou, Jiangsu 213001, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Department of Clinical Laboratory, Changzhou Children's Hospital, Changzhou, Jiangsu 213001, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, Changzhou Children's Hospital, Changzhou, Jiangsu 213001, China.
authors.forename: Zhi-Ping
authors.initials: ZP
authors.lastname: Che
authors.affiliation: Department of Clinical Laboratory, Changzhou Children's Hospital, Changzhou, Jiangsu 213001, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, Changzhou Children's Hospital, Changzhou, Jiangsu 213001, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhan
authors.affiliation: Department of Neonatal, Changzhou Children's Hospital, Changzhou, Jiangsu 213001, China.
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Tu