title: Simultaneous analysis of eight vitamin E isomers in Moringa oleifera Lam. leaves by ultra performance convergence chromatography.
abstract: A new method for simultaneous determination of eight vitamin E isomers including α-, β-, γ-, and δ-tocopherol and α-, β-, γ-, and δ-tocotrienol by ultra-performance convergence chromatography (UPC(2)) equipped with a diode array detector was reported. They were separated on a BEH 2-EP column (3.0 mm × 100 mm, 1.7 μm) using gradient elution (95:5-80:20) with a mobile phase consisted of CO2 and methanol:isopropanol (1:1, v/v), back pressure of 1800 psi, flow rate of 1.5 ml/min and detection at 294 nm. The results showed good linearity (R(2)=0.9990-0.9998) and high resolution (1.48-7.67). Limits of detection (LOD) and quantification (LOQ) ranged from 23-49 ng/L and 70-150 ng/L, respectively. Relative standard deviations (RSD) for repeatability and reproducibility were 0.62-3.16% and 0.82-3.34%, respectively. Moreover, this method was successfully applied to analysis the vitamin E isomers in Moringa oleifera leaf samples.
authors.affiliation: Chinese Academy of Tropical Agricultural Sciences Center for Food Quality Supervision and Testing Ministry of Agriculture (Zhanjiang), No. 48 Renmindadaonan, Zhanjiang 524001, China; Agricultural Product Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences, No. 48 Renmindadaonan, Zhanjiang 524001, China.
authors.forename: Ningli
authors.initials: N
authors.lastname: Qi
authors.affiliation: Agricultural Product Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences, No. 48 Renmindadaonan, Zhanjiang 524001, China. Electronic address: gongxiaocau@126.com.
authors.forename: Xiao
authors.initials: X
authors.lastname: Gong
authors.affiliation: College of Food Science and Engineering, Shanxi Agricultural University, Jinzhong 030801, China.
authors.forename: Cuiping
authors.initials: C
authors.lastname: Feng
authors.affiliation: Nutricia Pharmaceutical (Wuxi) Co., Ltd, No. 17 Xinming Rd, Wuxi 214111, China.
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Wang
authors.affiliation: Waters Corporation, Building 13, No. 1000 Jinhai Road, Pudong New District, Shanghai 201206, China.
authors.forename: Yongwei
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Agricultural Product Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences, No. 48 Renmindadaonan, Zhanjiang 524001, China.
authors.forename: Lijing
authors.initials: L
authors.lastname: Lin
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D007536:Isomerism; D057230:Limit of Detection; D029588:Moringa oleifera / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D015203:Reproducibility of Results; D014810:Vitamin E / Q000032:analysis* / Q000737:chemistry
chemical_list: D014810:Vitamin E
keywords: Moringa oleifera Lam. leaf; Tocopherols; Tocotrienols; UPC(2)