title: Genome-wide identification and expression analysis of the ClTCP transcription factors in Citrullus lanatus.
abstract: BACKGROUND
  The plant-specific TCP transcription factor family, which is involved in the regulation of cell growth and proliferation, performs diverse functions in multiple aspects of plant growth and development. However, no comprehensive analysis of the TCP family in watermelon (Citrullus lanatus) has been undertaken previously.
  
  
  RESULTS
  A total of 27 watermelon TCP encoding genes distributed on nine chromosomes were identified. Phylogenetic analysis clustered the genes into 11 distinct subgroups. Furthermore, phylogenetic and structural analyses distinguished two homology classes within the ClTCP family, designated Class I and Class II. The Class II genes were differentiated into two subclasses, the CIN subclass and the CYC/TB1 subclass. The expression patterns of all members were determined by semi-quantitative PCR. The functions of two ClTCP genes, ClTCP14a and ClTCP15, in regulating plant height were confirmed by ectopic expression in Arabidopsis wild-type and ortholog mutants.
  
  
  CONCLUSIONS
  This study represents the first genome-wide analysis of the watermelon TCP gene family, which provides valuable information for understanding the classification and functions of the TCP genes in watermelon.
authors.affiliation: Laboratory of Germplasm Innovation and Molecular Breeding, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310058, P.R. China.
authors.forename: Pibiao
authors.initials: P
authors.lastname: Shi
authors.affiliation: Laboratory of Germplasm Innovation and Molecular Breeding, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310058, P.R. China.|Faculty of Agronomy, Lubumbashi University, Lubumbashi, D.R. Congo.
authors.forename: Kateta Malangisha
authors.initials: KM
authors.lastname: Guy
authors.affiliation: Laboratory of Germplasm Innovation and Molecular Breeding, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310058, P.R. China.
authors.forename: Weifang
authors.initials: W
authors.lastname: Wu
authors.affiliation: Laboratory of Germplasm Innovation and Molecular Breeding, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310058, P.R. China.
authors.forename: Bingsheng
authors.initials: B
authors.lastname: Fang
authors.affiliation: Laboratory of Germplasm Innovation and Molecular Breeding, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310058, P.R. China.|Key laboratory of Horticultural Plant Growth, Development & Quality Improvement, Ministry of Agriculture, Hangzhou, 310058, P.R. China.|Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Hangzhou, 310058, P.R. China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yang
authors.affiliation: Laboratory of Germplasm Innovation and Molecular Breeding, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310058, P.R. China.|Key laboratory of Horticultural Plant Growth, Development & Quality Improvement, Ministry of Agriculture, Hangzhou, 310058, P.R. China.|Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Hangzhou, 310058, P.R. China.
authors.forename: Mingfang
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Laboratory of Germplasm Innovation and Molecular Breeding, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310058, P.R. China. huzhongyuan@zju.edu.cn.|Key laboratory of Horticultural Plant Growth, Development & Quality Improvement, Ministry of Agriculture, Hangzhou, 310058, P.R. China. huzhongyuan@zju.edu.cn.|Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Hangzhou, 310058, P.R. China. huzhongyuan@zju.edu.cn.
authors.forename: Zhongyuan
authors.initials: Z
authors.lastname: Hu
mesh_terms: D002716:Chlormequat / Q000494:pharmacology; D002874:Chromosome Mapping / Q000379:methods; D032461:Chromosomes, Plant / Q000235:genetics; D029773:Citrullus / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant / Q000235:genetics*; D005875:Gibberellins / Q000494:pharmacology; D005810:Multigene Family; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000145:classification / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D014157:Transcription Factors / Q000145:classification / Q000235:genetics*
chemical_list: D005875:Gibberellins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D014157:Transcription Factors; D002716:Chlormequat; C007842:gibberellic acid
keywords: Internode elongation; TCP; Transcription factors; Watermelon