title: Pre-clinical toxicology of garcinielliptone FC, a tautomeric pair of polyprenylated benzophenone, isolated from Platonia insignis Mart seeds.
abstract: BACKGROUND
  Garcinielliptone FC (GFC) is a tautomeric pair of polyprenylated benzophenone, which has proven to have antiepileptic, cytotoxic and antioxidant activity.
  
  
  OBJECTIVE
  The aim of this study was to investigate the biochemical, hematological and pathological effects of the acute toxicity study as well as to assess the locomotor activity and motor coordination in mice treated with GFC.
  
  
  METHODS
  Swiss mice of both sexes weighing 25-30 g divided into three separate groups of five animals matched by weight and size. GFC was aseptically suspended in 0.05% Tween 80, dissolved in 0.9% saline (vehicle) and administered orally (p.o.) and intraperitoneally (i.p.) (500, 1000 and 2000 mg/kg). The acute toxicity study was performed in compliance with the Anvisa regulations.
  
  
  RESULTS
  Behavioral manifestations of toxicity, such as state of consciousness, coordination, muscle tone, reflexes, the activity on the central nervous system (shake, seizures, Straub tail reaction and anesthesia) and the activity of the autonomic nervous system (lacrimation, ptosis, urination, piloerection, hypothermia, breathing and hyperemia) were not seen in any of the animals treated with doses of 500, 1000 and 2000 mg/kg. Additionally, no significant difference in body weight, food and water intake, excreta production or macroscopic changes in the organs of treated animals were detected in comparison with control group. GFC did not affect the locomotor activity and motor coordination of the animals.
  
  
  CONCLUSIONS
  The acute toxicity study indicated that GFC treatment, at selected doses given orally and intraperitoneally, showed relatively low risk of toxicity in all test animals, suggesting that it is safe for further investigation.
authors.affiliation: Department of Phamacy, Laboratory of Research in Experimental Neurochemistry (LAPNEX), Federal University of Piauí, Teresina, CEP 64049-550, Piauí, Brazil. Electronic address: apaulasantoslima@gmail.com.
authors.forename: Ana Paula dos Santos C L
authors.initials: AP
authors.lastname: da Silva
authors.affiliation: Department of Phamacy, Laboratory of Research in Experimental Neurochemistry (LAPNEX), Federal University of Piauí, Teresina, CEP 64049-550, Piauí, Brazil. Electronic address: georgenota10@hotmail.com.
authors.forename: George Laylson da Silva
authors.initials: GL
authors.lastname: Oliveira
authors.affiliation: Department of Phamacy, Laboratory of Research in Experimental Neurochemistry (LAPNEX), Federal University of Piauí, Teresina, CEP 64049-550, Piauí, Brazil.
authors.forename: Shirlene Cesario
authors.initials: SC
authors.lastname: Medeiros
authors.affiliation: Department of Phamacy, Laboratory of Research in Experimental Neurochemistry (LAPNEX), Federal University of Piauí, Teresina, CEP 64049-550, Piauí, Brazil.
authors.forename: Allyson Martins Lopes
authors.initials: AM
authors.lastname: Sousa
authors.affiliation: Department of Biophysics and Physiology, Federal University of Piaui, CEP 64000-040, Teresina, Piaui, Brazil.
authors.forename: Luciano da Silva
authors.initials: Lda S
authors.lastname: Lopes
authors.affiliation: Institute of Chemistry, CEP 40170-290, Federal University of Bahia, Salvador, Bahia, Brazil.
authors.forename: Jorge M
authors.initials: JM
authors.lastname: David
authors.affiliation: Department of Phamacy, Laboratory of Research in Experimental Neurochemistry (LAPNEX), Federal University of Piauí, Teresina, CEP 64049-550, Piauí, Brazil.
authors.forename: Joaquim S
authors.initials: JS
authors.lastname: da Costa Junior
authors.affiliation: Department of Phamacy, Laboratory of Research in Experimental Neurochemistry (LAPNEX), Federal University of Piauí, Teresina, CEP 64049-550, Piauí, Brazil.
authors.forename: Rivelilson Mendes
authors.initials: RM
authors.lastname: de Freitas
mesh_terms: D000818:Animals; D001577:Benzophenones / Q000737:chemistry*; D001835:Body Weight; D028084:Clusiaceae / Q000737:chemistry*; D005260:Female; D008297:Male; D051379:Mice; D015394:Molecular Structure; D009043:Motor Activity / Q000187:drug effects; D009048:Motor Skills / Q000187:drug effects; D009929:Organ Size; D012639:Seeds / Q000737:chemistry; D023382:Toxicity Tests, Acute; D014315:Triterpenes / Q000633:toxicity*
chemical_list: D001577:Benzophenones; D014315:Triterpenes; C573355:garcinielliptone FC
keywords: Acute toxicity; Garcinielliptone FC; Polyprenylated benzophenone