title: The Use of Kosher Phenotyping for Mapping QTL Affecting Susceptibility to Bovine Respiratory Disease.
abstract: Bovine respiratory disease (BRD) is the leading cause of morbidity and mortality in feedlot cattle, caused by multiple pathogens that become more virulent in response to stress. As clinical signs often go undetected and various preventive strategies failed, identification of genes affecting BRD is essential for selection for resistance. Selective DNA pooling (SDP) was applied in a genome wide association study (GWAS) to map BRD QTLs in Israeli Holstein male calves. Kosher scoring of lung adhesions was used to allocate 122 and 62 animals to High (Glatt Kosher) and Low (Non-Kosher) resistant groups, respectively. Genotyping was performed using the Illumina BovineHD BeadChip according to the Infinium protocol. Moving average of -logP was used to map QTLs and Log drop was used to define their boundaries (QTLRs). The combined procedure was efficient for high resolution mapping. Nineteen QTLRs distributed over 13 autosomes were found, some overlapping previous studies. The QTLRs contain polymorphic functional and expression candidate genes to affect kosher status, with putative immunological and wound healing activities. Kosher phenotyping was shown to be a reliable means to map QTLs affecting BRD morbidity.
authors.affiliation: Department of Genetics, Hebrew University of Jerusalem, Jerusalem, Israel.
authors.forename: Ehud
authors.identifier: http://orcid.org/0000-0003-2624-6765
authors.initials: E
authors.lastname: Lipkin
authors.affiliation: Università degli Studi di Milano (UNIMI), Milan, Italy.
authors.forename: Maria Giuseppina
authors.identifier: http://orcid.org/0000-0002-8972-1730
authors.initials: MG
authors.lastname: Strillacci
authors.affiliation: Department of Ruminant Sciences, Agricultural Research Organization (ARO), Bet-Dagan, Israel.
authors.forename: Harel
authors.initials: H
authors.lastname: Eitam
authors.affiliation: Department of Ruminant Sciences, Agricultural Research Organization (ARO), Bet-Dagan, Israel.
authors.forename: Moran
authors.initials: M
authors.lastname: Yishay
authors.affiliation: Università degli Studi di Milano (UNIMI), Milan, Italy.
authors.forename: Fausta
authors.initials: F
authors.lastname: Schiavini
authors.affiliation: Department of Genetics, Hebrew University of Jerusalem, Jerusalem, Israel.
authors.forename: Morris
authors.initials: M
authors.lastname: Soller
authors.affiliation: Università degli Studi di Milano (UNIMI), Milan, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Bagnato
authors.affiliation: Department of Ruminant Sciences, Agricultural Research Organization (ARO), Bet-Dagan, Israel.
authors.forename: Ariel
authors.initials: A
authors.lastname: Shabtay
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics* / Q000276:immunology; D002874:Chromosome Mapping; D060467:Disease Resistance / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics; D008297:Male; D010641:Phenotype; D040641:Quantitative Trait Loci / Q000235:genetics*; D012140:Respiratory Tract Diseases / Q000235:genetics / Q000276:immunology / Q000662:veterinary*