title: A Pycnoporus sanguineus laccase for denim bleaching and its comparison with an enzymatic commercial formulation.
abstract: A laccase from the basidiomycete Pycnoporus sanguineus strain RVAN5 was evaluated for its ability to decolorize synthetic dyes and denim bleaching. Dye color reduction and denim bleaching were monitored at different dye concentrations and incubation times. Dye decolorization by Pycnoporus sanguineus fungal crude extract (FCE) ranged from 80 to 96% within 2-4 h at 25-65 °C. Comparable results were obtained when violuric acid (VA) was added as mediator to the FCE, however, the number of decolorized dyes increased significantly. Dye decolorization rates with VA varied of initial and final optical density (595 nm) values of 2.5-3.0 and 0.2-0.02, respectively. P. sanguineus FCE had no substantial effect on denim bleaching when used alone, notwithstanding, the mixture of FCE with VA (10 mM) showed significant denim color reduction values and considerably higher than those obtained with a bleaching enzyme from a commercial formulation; CIElab values obtained with FCE/VA mixture were of ΔL = 6.4, versus a ΔL 1.4 value obtained with an enzyme from commercial formulation.
authors.affiliation: Instituto de Biotecnología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Pedro de Alba s/n, C.P. 66455, Ciudad Universitaria, San Nicolás de Los Garza, Nuevo León, Mexico. Electronic address: maria.irachetacr@uanl.edu.mx.
authors.forename: María Magdalena
authors.initials: MM
authors.lastname: Iracheta-Cárdenas
authors.affiliation: Instituto de Biotecnología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Pedro de Alba s/n, C.P. 66455, Ciudad Universitaria, San Nicolás de Los Garza, Nuevo León, Mexico. Electronic address: mario.rochape@uanl.edu.mx.
authors.forename: Mario A
authors.initials: MA
authors.lastname: Rocha-Peña
authors.affiliation: Instituto de Biotecnología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Pedro de Alba s/n, C.P. 66455, Ciudad Universitaria, San Nicolás de Los Garza, Nuevo León, Mexico. Electronic address: luis.galanwn@uanl.edu.mx.
authors.forename: Luis J
authors.initials: LJ
authors.lastname: Galán-Wong
authors.affiliation: Instituto de Biotecnología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Pedro de Alba s/n, C.P. 66455, Ciudad Universitaria, San Nicolás de Los Garza, Nuevo León, Mexico. Electronic address: katiushka.arevalonn@uanl.edu.mx.
authors.forename: Katiushka
authors.initials: K
authors.lastname: Arévalo-Niño
authors.affiliation: Instituto de Biotecnología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Pedro de Alba s/n, C.P. 66455, Ciudad Universitaria, San Nicolás de Los Garza, Nuevo León, Mexico. Electronic address: omar.tovarhr@uanl.edu.mx.
authors.forename: Omar Eduardo
authors.initials: OE
authors.lastname: Tovar-Herrera
mesh_terms: D001463:Barbiturates / Q000737:chemistry; D003116:Color; D004396:Coloring Agents / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism; D042845:Laccase / Q000737:chemistry* / Q000378:metabolism*; D055494:Pycnoporus / Q000737:chemistry / Q000201:enzymology*; D013784:Textiles
chemical_list: D001463:Barbiturates; D004396:Coloring Agents; D005656:Fungal Proteins; C094277:violuric acid; D042845:Laccase
keywords: CIE lab; Denim bleaching; Dye decolorization; Laccase activity; Pycnoporus