title: Polaromonas eurypsychrophila sp. nov., isolated from an ice core.
abstract: A Gram-stain-negative, aerobic, rod-shaped, beige bacterium, strain B717-2T, was isolated from an ice core drilled from Muztagh Glacier on the Tibetan Plateau, China. According to phylogenetic analyses based on 16S rRNA gene sequences, the novel strain was related most closely to Polaromonas vacuolataand shared 97.7 % similarity with the type strain of this species. It grew optimally at pH 7, at 15 °C and with 2 % (w/v) NaCl. Major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The major fatty acids were summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), summed feature 8 (C18 : 1ω7c, C18 : 1ω6c) and C16 : 0. The sole respiratory quinone was Q-8. The DNA G+C content was 63.4 mol %. In DNA-DNA hybridization tests, strain B717-2T shared 37.0±1.9, 30.0±1.7, 26.0±0.9, 23.4±0.5 and 18.4±1.9 % DNA-DNA relatedness with Polaromonas jejuensisJS12-13T, P. vacuolata 34-PT, Polaromonas aquatica CCUG 39402T, Polaromonas glacialisCr4-12T and Polaromonas cryoconitiCr4-35T, respectively. Based on the phenotypic, phylogenetic and genetic characteristics, strain B717-2T represents a novel species of the genus Polaromonas, for which the name Polaromonaseurypsychrophila sp. nov. is proposed. The type strain is B717-2T (=CGMCC 1.15322T=JCM 31171T).
authors.affiliation: Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Xing
authors.affiliation: CAS Center for Excellence in Tibetan Plateau Earth Sciences, Chinese Academy of Sciences, Beijing 100101, China.|Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Tandong
authors.initials: T
authors.lastname: Yao
authors.affiliation: CAS Center for Excellence in Tibetan Plateau Earth Sciences, Chinese Academy of Sciences, Beijing 100101, China.|Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yongqin
authors.initials: Y
authors.lastname: Liu
authors.affiliation: CAS Center for Excellence in Tibetan Plateau Earth Sciences, Chinese Academy of Sciences, Beijing 100101, China.|College of Urban and Environmental Science, Northwest University, Xi'an 710000, China.
authors.forename: Ninglian
authors.initials: N
authors.lastname: Wang
authors.affiliation: CAS Center for Excellence in Tibetan Plateau Earth Sciences, Chinese Academy of Sciences, Beijing 100101, China.|Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Bainqing
authors.initials: B
authors.lastname: Xu
authors.affiliation: Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Shen
authors.affiliation: Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Zhengquan
authors.initials: Z
authors.lastname: Gu
authors.affiliation: Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Bixi
authors.initials: B
authors.lastname: Gu
authors.affiliation: Institute of Microbiology, China General Microbiological Culture Collection Center, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Hongcan
authors.initials: H
authors.lastname: Liu
authors.affiliation: Institute of Microbiology, China General Microbiological Culture Collection Center, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D042621:Comamonadaceae / Q000737:chemistry / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D046448:Ice Cover / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S