title: A novel and highly sensitive nanocatalytic surface plasmon resonance-scattering analytical platform for detection of trace Pb ions.
abstract: Gold nanoparticles (AuNP) have catalysis on the reaction of HAuCl4-H2O2. The produced AuNP have strong resonance Rayleigh scattering (RRS) effect and surface-enhanced resonance Raman scattering (SERS) effect when Victoria blue B (VBB) and rhodamine S (RhS) were used as probes. The increased RRS/SERS intensity respond linearly with the concentration of gold nanoparticles (AuNPB) which synthesized by NaBH4 over 0.038-76 ng/mL, 19-285 ng/mL, 3.8-456 ng/mL respectively. Four kinds of tested nanoparticles have catalysis on the HAuCl4-H2O2 particles reaction. Thus, a novel nanocatalysis surface plasmon resonance-scattering (SPR-S) analytical platform was developed for AuNP. The DNAzyme strand hybridized with the substrate strand to form double-stranded DNA (dsDNA) which couldn't protect AuNPc to aggregate to AuNPc aggregations, having strong RRS effect. Upon addition of Pb(2+), dsDNA could be cracked by Pb(2+) to produce single-stranded DNA (ssDNA) that adsorbed on the AuNPc surface to form AuNPc-ssDNA conjugates. The conjugates have strong catalysis on HAuCl4-H2O2 reaction. With increased Pb(2+) concentration, the concentration of AuNPc-ssDNA increased and lead to the catalytic activity stronger. The increased RRS intensity responds linearly with Pb(2+) concentration over 16.7-666.7 nmol/L. The SERS intensity responded linearly with the concentration of Pb(2+) over 50-500 nmol/L.
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry Education, Guangxi Normal University, Guilin 541004, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Ye
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry Education, Guangxi Normal University, Guilin 541004, China.
authors.forename: Guiqing
authors.initials: G
authors.lastname: Wen
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry Education, Guangxi Normal University, Guilin 541004, China.
authors.forename: Huixiang
authors.initials: H
authors.lastname: Ouyang
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry Education, Guangxi Normal University, Guilin 541004, China.
authors.forename: Qingye
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry Education, Guangxi Normal University, Guilin 541004, China.
authors.forename: Aihui
authors.initials: A
authors.lastname: Liang
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry Education, Guangxi Normal University, Guilin 541004, China.
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Jiang
mesh_terms: D002384:Catalysis; D006046:Gold / Q000737:chemistry; D007854:Lead / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*
chemical_list: D007854:Lead; D006046:Gold