title: [Dental pulp stem cells modified by HIF-1α can differentiate into blood vessels].
abstract: OBJECTIVE
  To investigate the method of differentiating dental pulp stem cells (DPSCs) modified by HIF-1α into blood vessels.
  
  
  METHODS
  DPSCs were extracted from teeth samples from 20 patients and were identified by Strol-1 and CD146. DPSCs were divided into experimental group and control group according to DPSCs were modified by HIF-1α not or. HIF-1α-mRNA expression was detected by RT-PCR. HIF-1α, VEGF, SDF-1, Ang-2 and PDGF expression were detected using Western blot in different time after culture for 1 d, 4 d, 7 d and 14 d. Statistical analysis was carried out with SPSS 16.0 software package.
  
  
  RESULTS
  Most DPSCs appeared round, oval under phase-contrast microscopy. CD146 and Strol-1 showed green fluorescence. HIF-1α and HIF-1α-mRNA expression became higher with time passing and the difference was statistically significant (P<0.05). Compared with the control group, HIF-1α protein and mRNA increased obviously in the experimental group 1d, 4d, 7d and 14d after transfection, and the difference was statistically significant (P<0.05). The level of VEGF, SDF-1, Ang-2 and PDGF in the control group was changed unconspicuously, and the expression was not different at different times (P>0.05). The level of VEGF, SDF-1, Ang-2 and PDGF in the exprimental group increased, and the difference was statistically significant between different time points(P<0.05). Compared with the control group, the level of VEGF, SDF-1, Ang-2 and PDGF in the experimental group was higher 1 d, 4 d, 7 d and 14 d after transfection, respectively, and the difference was statistically significant(P<0.05).
  
  
  CONCLUSIONS
  DPSCs modified by HIF-1α gene can successfully induce vascular differentiation in vitro, which provides foundation for further angioplasty.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Binzhou Medical University. Binzhou 256603, China. E-mail:renxinmiaoshu@hotmail.com.
authors.forename: Hong-hai
authors.initials: HH
authors.lastname: Fu
authors.affiliation: Department of Stomatology, Binzhou People's Hospital. Binzhou 256603, Shandong Province, China.
authors.forename: Peng-chong
authors.initials: PC
authors.lastname: Li
authors.affiliation: Department of Stomatology, Binzhou People's Hospital. Binzhou 256603, Shandong Province,China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Binzhou Medical University. Binzhou 256603 China.
authors.forename: Jin-hua
authors.initials: JH
authors.lastname: Zuo
mesh_terms: D002454:Cell Differentiation; D054377:Chemokine CXCL12; D003782:Dental Pulp / Q000166:cytology*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D012333:RNA, Messenger; D013234:Stem Cells; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A