title: miR-149 controls non-alcoholic fatty liver by targeting FGF-21.
abstract: Non-alcoholic fatty liver disease (NAFLD), a lipid metabolism disorder characterized by the accumulation of intrahepatic fat, has emerged as a global public health problem. However, its underlying molecular mechanism remains unclear. We previously have found that miR-149 was elevated in NAFLD induced by high-fat diet mice model, whereas decreased by a 16-week running programme. Here, we reported that miR-149 was increased in HepG2 cells treated with long-chain fatty acid (FFA). In addition, miR-149 was able to promote lipogenesis in HepG2 cells in the absence of FFA treatment. Moreover, inhibition of miR-149 was capable of inhibiting lipogenesis in HepG2 cells in the presence of FFA treatment. Meanwhile, fibroblast growth factor-21 (FGF-21) was identified as a target gene of miR-149, which was demonstrated by the fact that miR-149 could negatively regulate the protein expression level of FGF-21, and FGF-21 was also responsible for the effect of miR-149 inhibitor in decreasing lipogenesis in HepG2 cells in the presence of FFA treatment. These data implicate that miR-149 might be a novel therapeutic target for NAFLD.
authors.affiliation: Regeneration and Ageing Lab, Experimental Center of Life Sciences, School of Life Science, Shanghai University, Shanghai, China.|Innovative Drug Research Center of Shanghai University, Shanghai, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Regeneration and Ageing Lab, Experimental Center of Life Sciences, School of Life Science, Shanghai University, Shanghai, China.|Innovative Drug Research Center of Shanghai University, Shanghai, China.
authors.forename: Dongchao
authors.initials: D
authors.lastname: Lv
authors.affiliation: Division of Gastroenterology and Hepatology, Digestive Disease Institute, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Division of Gastroenterology and Hepatology, Digestive Disease Institute, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Chen
authors.affiliation: Division of Gastroenterology and Hepatology, Digestive Disease Institute, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Meiyi
authors.initials: M
authors.lastname: Song
authors.affiliation: Division of Gastroenterology and Hepatology, Digestive Disease Institute, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Jingqi
authors.initials: J
authors.lastname: Liu
authors.affiliation: Regeneration and Ageing Lab, Experimental Center of Life Sciences, School of Life Science, Shanghai University, Shanghai, China.|Innovative Drug Research Center of Shanghai University, Shanghai, China.
authors.forename: Yihua
authors.initials: Y
authors.lastname: Bei
authors.affiliation: Division of Gastroenterology and Hepatology, Digestive Disease Institute, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Division of Gastroenterology and Hepatology, Digestive Disease Institute, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Wenzhuo
authors.initials: W
authors.lastname: Yang
authors.affiliation: Division of Gastroenterology and Hepatology, Digestive Disease Institute, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Changqing
authors.initials: C
authors.lastname: Yang
mesh_terms: D005227:Fatty Acids; D005346:Fibroblast Growth Factors / Q000235:genetics* / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D066292:Lipid Droplets / Q000378:metabolism; D050155:Lipogenesis; D035683:MicroRNAs / Q000378:metabolism*; D065626:Non-alcoholic Fatty Liver Disease / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D005227:Fatty Acids; C551557:MIRN149 microRNA, human; D035683:MicroRNAs; C414620:fibroblast growth factor 21; D005346:Fibroblast Growth Factors
keywords: Non-alcoholic fatty liver disease; fibroblast growth factor-21; miR-149