title: CD24 negative lung cancer cells, possessing partial cancer stem cell properties, cannot be considered as cancer stem cells.
abstract: Cancer stem cells (CSCs) play vital role in lung cancer progression, resistance, metastasis and relapse. Identifying lung CSCs makers for lung CSCs targeting researches are critical for lung cancer therapy. In this study, utilizing previous identified lung CSCs as model, we compared the expression of CD24, CD133 and CD44 between CSCs and non-stem cancer cells. Increased ratio of CD24- cells were found in CSCs. CD24- cells were then sorted by flow cytometry and their proliferative ability, chemo-resistance property and in vivo tumor formation abilities were detected. A549 CD24- cells formed smaller colonies, slower proliferated in comparison to A549 CD24+ cells. Besides, A549 CD24- exhibited stronger resistance to chemotherapy drug. However, A549 CD24- didn't exert any stronger tumor formation ability in vivo, which is the gold standard of CSCs. These results showed that CD24- A549 cells showed some properties of CSCs but not actually CSCs. This study provides evidence that CD24 cannot be considered as lung CSCs marker.
authors.affiliation: State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesShanghai 200031, China; Department of Radiation Oncology, School of Medicine, University of PennsylvaniaPhiladelphia, PA 19104, USA.
authors.forename: Haineng
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of General Surgery, Xinhua Hospital, Affiliated to School of Medicine, Shanghai Jiao Tong UniversityShanghai 200092, China; Institute of Biliary Tract Disease, School of Medicine, Shanghai Jiao Tong UniversityShanghai 200092, China.
authors.forename: Jiasheng
authors.initials: J
authors.lastname: Mu
authors.affiliation: State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Shanghai 200031, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Department of General Surgery, Xinhua Hospital, Affiliated to School of Medicine, Shanghai Jiao Tong UniversityShanghai 200092, China; Institute of Biliary Tract Disease, School of Medicine, Shanghai Jiao Tong UniversityShanghai 200092, China.
authors.forename: Xiangsong
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of General Surgery, Xinhua Hospital, Affiliated to School of Medicine, Shanghai Jiao Tong UniversityShanghai 200092, China; Institute of Biliary Tract Disease, School of Medicine, Shanghai Jiao Tong UniversityShanghai 200092, China.
authors.forename: Maolan
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Otorinolaryngology Head and Neck Surgery, The Sixth Affiliated Hospital of Sun Yat-sen University Guangzhou, Guangdong, China.
authors.forename: Tianrun
authors.initials: T
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Shanghai 200031, China.
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Liu
keywords: A549; CD133; CD24; CD44; Lung cancer; biomarker; cancer stem cell marker; cancer stem cells