title: Pharmacokinetics study of mazindol in plasma, oral fluid, and urine of volunteers.
abstract: OBJECTIVE
  There are no pharmacokinetics studies in oral fluid reported in the literature, as well as there are no data on correlation of drug levels in plasma, urine, and oral fluid in order to propose alternative matrices to monitor the use of mazindol by drivers. The present work aimed to study, preliminarily, mazindol's pharmacokinetics in plasma and oral fluid, as well as investigate the correlation of drug levels in urine, plasma, and oral fluid.
  
  
  METHODS
  Blood, urine, and oral fluid samples from seven healthy male volunteers were collected at 0, 1, 2, 4, 5, 6, 8, 10, and 24 h after administration of tablets of 2 mg mazindol and analyzed by a previously validated method by LC-MS with liquid-liquid extraction. Levels of the drug found were higher in plasma when compared with oral fluid and higher in urine in relation to plasma. The study of the mazindol's pharmacokinetics showed that the most suitable model to describe the variation of the concentration over time is the compartment open model with absorption and elimination following the first-order kinetics, and confirming literature data, drug is metabolized, being the major metabolite detected, but not quantified.
  
  
  CONCLUSIONS
  It was not found a good correlation between the concentrations of mazindol in urine and plasma, but between plasma and oral fluid, there was a good correlation, suggesting this as an alternative matrix to plasma. However, studies involving more subjects are needed.
authors.affiliation: Programa de Pós Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do sul, Avenida Ipiranga, 2752, Santana, Porto Alegre, Rio Grande do Sul, CEP: 90610-000, Brazil.
authors.forename: Marcella Herbstrith
authors.initials: MH
authors.lastname: de Oliveira
authors.affiliation: Programa de Pós Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do sul, Avenida Ipiranga, 2752, Santana, Porto Alegre, Rio Grande do Sul, CEP: 90610-000, Brazil. pamlukasewicz@gmail.com.
authors.forename: Pâmela Cristina Lukasewicz
authors.initials: PC
authors.lastname: Ferreira
authors.affiliation: Programa de Pós Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do sul, Avenida Ipiranga, 2752, Santana, Porto Alegre, Rio Grande do Sul, CEP: 90610-000, Brazil.
authors.forename: Graciela
authors.initials: G
authors.lastname: Carlos
authors.affiliation: Programa de Pós Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do sul, Avenida Ipiranga, 2752, Santana, Porto Alegre, Rio Grande do Sul, CEP: 90610-000, Brazil.
authors.forename: Fernanda Rodrigues
authors.initials: FR
authors.lastname: Salazar
authors.affiliation: Programa de Pós Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do sul, Avenida Ipiranga, 2752, Santana, Porto Alegre, Rio Grande do Sul, CEP: 90610-000, Brazil.
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Bergold
authors.affiliation: Centro de Pesquisa Álcool e Drogas (CPAD), Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Rua Álvaro Alvim, 400, Porto Alegre, Rio Grande do Sul, CEP: 90420-020, Brazil.
authors.forename: Flavio
authors.initials: F
authors.lastname: Pechansky
authors.affiliation: Programa de Pós Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do sul, Avenida Ipiranga, 2752, Santana, Porto Alegre, Rio Grande do Sul, CEP: 90610-000, Brazil.
authors.forename: Renata Pereira
authors.initials: RP
authors.lastname: Limberger
authors.affiliation: Programa de Pós Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do sul, Avenida Ipiranga, 2752, Santana, Porto Alegre, Rio Grande do Sul, CEP: 90610-000, Brazil.
authors.forename: Pedro Eduardo
authors.initials: PE
authors.lastname: Fröehlich
mesh_terms: D000284:Administration, Oral; D000328:Adult; D000697:Central Nervous System Stimulants / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D064368:Healthy Volunteers; D006801:Humans; D008297:Male; D008454:Mazindol / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D008954:Models, Biological; D012463:Saliva / Q000737:chemistry; D055815:Young Adult
chemical_list: D000697:Central Nervous System Stimulants; D008454:Mazindol
keywords: LC/MS; Mazindol; Oral fluid; Pharmacokinetics; Plasma; Urine