title: Visualization of neonatal lung injury associated with mechanical ventilation using x-ray dark-field radiography.
abstract: Mechanical ventilation (MV) and supplementation of oxygen-enriched gas, often needed in postnatal resuscitation procedures, are known to be main risk factors for impaired pulmonary development in the preterm and term neonates. Unfortunately, current imaging modalities lack in sensitivity for the detection of early stage lung injury. The present study reports a new imaging approach for diagnosis and staging of early lung injury induced by MV and hyperoxia in neonatal mice. The imaging method is based on the Talbot-Lau x-ray grating interferometry that makes it possible to quantify the x-ray small-angle scattering on the air-tissue interfaces. This so-called dark-field signal revealed increasing loss of x-ray small-angle scattering when comparing images of neonatal mice undergoing hyperoxia and MV-O2 with animals kept at room air. The changes in the dark field correlated well with histologic findings and provided superior differentiation than conventional x-ray imaging and lung function testing. The results suggest that x-ray dark-field radiography is a sensitive tool for assessing structural changes in the developing lung. In the future, with further technical developments x-ray dark-field imaging could be an important tool for earlier diagnosis and sensitive monitoring of lung injury in neonates requiring postnatal oxygen or ventilator therapy.
authors.affiliation: Lehrstuhl für Biomedizinische Physik, Physik-Department &Institut für Medizintechnik, Technische Universität München, Garching, Germany.
authors.forename: Andre
authors.initials: A
authors.lastname: Yaroshenko
authors.affiliation: Comprehensive Pneumology Center, Helmholtz Zentrum Muenchen, Munich, Germany, Member of the German Center for Lung Research (DZL).
authors.forename: Tina
authors.initials: T
authors.lastname: Pritzke
authors.affiliation: Comprehensive Pneumology Center, Helmholtz Zentrum Muenchen, Munich, Germany, Member of the German Center for Lung Research (DZL).
authors.forename: Markus
authors.initials: M
authors.lastname: Koschlig
authors.affiliation: Comprehensive Pneumology Center, Helmholtz Zentrum Muenchen, Munich, Germany, Member of the German Center for Lung Research (DZL).
authors.forename: Nona
authors.initials: N
authors.lastname: Kamgari
authors.affiliation: Lehrstuhl für Biomedizinische Physik, Physik-Department &Institut für Medizintechnik, Technische Universität München, Garching, Germany.
authors.forename: Konstantin
authors.initials: K
authors.lastname: Willer
authors.affiliation: Lehrstuhl für Biomedizinische Physik, Physik-Department &Institut für Medizintechnik, Technische Universität München, Garching, Germany.
authors.forename: Lukas
authors.initials: L
authors.lastname: Gromann
authors.affiliation: Institute for Clinical Radiology, Ludwig-Maximilians-University Hospital Munich, Munich.
authors.forename: Sigrid
authors.initials: S
authors.lastname: Auweter
authors.affiliation: Institute for Clinical Radiology, Ludwig-Maximilians-University Hospital Munich, Munich.
authors.forename: Katharina
authors.initials: K
authors.lastname: Hellbach
authors.affiliation: Institute for Clinical Radiology, Ludwig-Maximilians-University Hospital Munich, Munich.
authors.forename: Maximilian
authors.initials: M
authors.lastname: Reiser
authors.affiliation: Comprehensive Pneumology Center, Helmholtz Zentrum Muenchen, Munich, Germany, Member of the German Center for Lung Research (DZL).
authors.forename: Oliver
authors.initials: O
authors.lastname: Eickelberg
authors.affiliation: Lehrstuhl für Biomedizinische Physik, Physik-Department &Institut für Medizintechnik, Technische Universität München, Garching, Germany.
authors.forename: Franz
authors.initials: F
authors.lastname: Pfeiffer
authors.affiliation: Comprehensive Pneumology Center, Helmholtz Zentrum Muenchen, Munich, Germany, Member of the German Center for Lung Research (DZL).|Department of Neonatology, Perinatal Center, Dr. von Haunersches Children's Hospital, Ludwig-Maximilians University, Munich, Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Hilgendorff
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D055370:Lung Injury / Q000000981:diagnostic imaging* / Q000209:etiology; D051379:Mice; D008810:Mice, Inbred C57BL; D012121:Respiration, Artificial / Q000009:adverse effects*