title: SMARCA4/Brg1 coordinates genetic and epigenetic networks underlying Shh-type medulloblastoma development.
abstract: Recent large-scale genomic studies have classified medulloblastoma into four subtypes: Wnt, Shh, Group 3 and Group 4. Each is characterized by specific mutations and distinct epigenetic states. Previously, we showed that a chromatin regulator SMARCA4/Brg1 is required for Gli-mediated transcription activation in Sonic hedgehog (Shh) signaling. We report here that Brg1 controls a transcriptional program that specifically regulates Shh-type medulloblastoma growth. Using a mouse model of Shh-type medulloblastoma, we deleted Brg1 in precancerous progenitors and primary or transplanted tumors. Brg1 deletion significantly inhibited tumor formation and progression. Genome-wide expression analyses and binding experiments indicate that Brg1 specifically coordinates with key transcription factors including Gli1, Atoh1 and REST to regulate the expression of both oncogenes and tumor suppressors that are required for medulloblastoma identity and proliferation. Shh-type medulloblastoma displays distinct H3K27me3 properties. We demonstrate that Brg1 modulates activities of H3K27me3 modifiers to regulate the expression of medulloblastoma genes. Brg1-regulated pathways are conserved in human Shh-type medulloblastoma, and Brg1 is important for the growth of a human medulloblastoma cell line. Thus, Brg1 coordinates a genetic and epigenetic network that regulates the transcriptional program underlying the Shh-type medulloblastoma development.
authors.affiliation: Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
authors.forename: X
authors.initials: X
authors.lastname: Shi
authors.affiliation: Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Biological Sciences, Center for Systems Biology, University of Texas at Dallas, Richardson, TX, USA.
authors.forename: J
authors.initials: J
authors.lastname: Gu
authors.affiliation: Department of Biological Sciences, Center for Systems Biology, University of Texas at Dallas, Richardson, TX, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Xuan
authors.affiliation: Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
authors.forename: J I
authors.initials: JI
authors.lastname: Wu
mesh_terms: D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism; D002528:Cerebellar Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D004265:DNA Helicases / Q000378:metabolism*; D004195:Disease Models, Animal; D018450:Disease Progression; D044127:Epigenesis, Genetic; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D053823:Hedgehog Proteins / Q000378:metabolism*; D064593:Heterografts; D006657:Histones / Q000378:metabolism; D006801:Humans; D008527:Medulloblastoma / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008954:Models, Biological; D009687:Nuclear Proteins / Q000378:metabolism*; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D053823:Hedgehog Proteins; D006657:Histones; D009687:Nuclear Proteins; D014157:Transcription Factors; C084108:SMARCA4 protein, human; D004265:DNA Helicases