title: MicroRNA-193b inhibits the proliferation, migration and invasion of gastric cancer cells via targeting cyclin D1.
abstract: MicroRNAs have been involved in RNA silencing and post-transcriptional regulation of gene expression and also play important roles in tumorigenesis and tumor progression. MiR-193b was previously reported to act as tumor suppressor in diverse cancers. However, little is known about the expression, function and mechanism of miR-193b in gastric cancer (GC). In this study, we investigated the expression of miR-193b in 50 GC cases and found that miR-193b was significantly reduced in GC tissues compared with the adjacent normal gastric tissues. Moreover, lower-level of miR-193b was also associated with a more aggressive GC phenotype. We further demonstrated that miR-193b can inhibit the proliferation, migration and invasion of HGC-27 and MGC-803 GC cells. Further mechanism study indicated that CCND1 was a direct target of miR-193b in GC. Overexpression of miR-193b inhibited the expression of CCND1, and knock-down of CCND1 inhibited the proliferation of GC cells, suggesting that miR-193b exerted its anti-tumorigenic role in GC cells through targeting CCND1 gene.
authors.affiliation: Institute of Molecular Medicine, Medical School, Henan University, KaiFeng 475000, China; Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing 100005, China.
authors.forename: Lanlan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Institute of Molecular Medicine, Medical School, Henan University, KaiFeng 475000, China; Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing 100005, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Molecular Medicine, Medical School, Henan University, KaiFeng 475000, China; Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing 100005, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing 100005, China.
authors.forename: Siqi
authors.initials: S
authors.lastname: Liu
authors.affiliation: Institute of Molecular Medicine, Medical School, Henan University, KaiFeng 475000, China.
authors.forename: Shashuang
authors.initials: S
authors.lastname: Yu
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing 100005, China.
authors.forename: Yanni
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Institute of Molecular Medicine, Medical School, Henan University, KaiFeng 475000, China. Electronic address: guotao_sun@hedu.edu.cn.
authors.forename: Guotao
authors.initials: G
authors.lastname: Sun
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: C530104:CCND1 protein, human; C546818:MIRN193 microRNA, human; D035683:MicroRNAs; D019938:Cyclin D1
keywords: CCND1; Gastric cancer; MicroRNA; miR-193b