title: MicroRNA-351 Regulates Two-Types of Cell Death, Necrosis and Apoptosis, Induced by 5-fluoro-2'-deoxyuridine.
abstract: Cell-death can be necrosis and apoptosis. We are investigating the mechanisms regulating the cell death that occurs on treatment of mouse cancer cell-line FM3A with antitumor 5-fluoro-2'-deoxyuridine (FUdR): necrosis occurs for the original clone F28-7, and apoptosis for its variant F28-7-A. Here we report that a microRNA (miR-351) regulates the cell death pattern. The miR-351 is expressed strongly in F28-7-A but only weakly in F28-7. Induction of a higher expression of miR-351 in F28-7 by transfecting an miRNA mimic into F28-7 resulted in a change of the death mode; necrosis to apoptosis. Furthermore, transfection of an miR-351 inhibitor into F28-7-A resulted in the morphology change, apoptosis to necrosis, in this death-by-FUdR. Possible mechanism involving lamin B1 in this miR-351's regulatory action is discussed.
authors.affiliation: Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Yamazaki, Noda, Chiba, Japan.|Division of Genome Stability Research, National Cancer Center Research Institute, Tsukiji, Chuo-ku, Tokyo, Japan.|Division of Chemotherapy and Clinical Research, National Cancer Center Research Institute, Tsukiji, Chuo-ku, Tokyo, Japan.|Department of Drug Informatics, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-naka, Kita-ku, Okayama, Japan.
authors.forename: Akira
authors.identifier: http://orcid.org/0000-0002-2239-6208
authors.initials: A
authors.lastname: Sato
authors.affiliation: Department of Drug Informatics, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-naka, Kita-ku, Okayama, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Omi
authors.affiliation: Department of Drug Informatics, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-naka, Kita-ku, Okayama, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Yamamoto
authors.affiliation: Department of Drug Informatics, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-naka, Kita-ku, Okayama, Japan.
authors.forename: Akito
authors.initials: A
authors.lastname: Satake
authors.affiliation: Department of Drug Informatics, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-naka, Kita-ku, Okayama, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Hiramoto
authors.affiliation: Division of Genome Stability Research, National Cancer Center Research Institute, Tsukiji, Chuo-ku, Tokyo, Japan.|Division of Chemotherapy and Clinical Research, National Cancer Center Research Institute, Tsukiji, Chuo-ku, Tokyo, Japan.|Department of Frontier Life Sciences, Nagasaki University Graduate School of Biomedical Sciences, Sakamoto, Nagasaki, Japan.
authors.forename: Mitsuko
authors.initials: M
authors.lastname: Masutani
authors.affiliation: Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Yamazaki, Noda, Chiba, Japan.
authors.forename: Sei-ichi
authors.initials: S
authors.lastname: Tanuma
authors.affiliation: Department of Drug Informatics, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-naka, Kita-ku, Okayama, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Wataya
authors.affiliation: Department of Drug Informatics, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-naka, Kita-ku, Okayama, Japan.
authors.forename: Hye-Sook
authors.initials: HS
authors.lastname: Kim
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics*; D016923:Cell Death / Q000187:drug effects* / Q000235:genetics*; D045744:Cell Line, Tumor; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000494:pharmacology; D020869:Gene Expression Profiling; D034921:Lamin Type B / Q000235:genetics / Q000378:metabolism; D051379:Mice; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism*; D018716:Molecular Mimicry; D009336:Necrosis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D015854:Up-Regulation
chemical_list: C576827:5-fluoro-2'-deoxyuridine; D000970:Antineoplastic Agents; D034921:Lamin Type B; C580764:MIRN351 microRNA, 351; D035683:MicroRNAs; D012333:RNA, Messenger; D003857:Deoxyuridine