title: Pyrogenic transformation of Nannochloropsis oceanica into fatty acid methyl esters without oil extraction for estimating total lipid content.
abstract: This study fundamentally investigated the pseudo-catalytic transesterification of dried Nannochloropsis oceanica into fatty acid methyl esters (FAMEs) without oil extraction, which was achieved in less than 5min via a thermo-chemical pathway. This study presented that the pseudo-catalytic transesterification reaction was achieved in the presence of silica and that its main driving force was identified as temperature: pores in silica provided the numerous reaction space like a micro-reactor, where the heterogeneous reaction was developed. The introduced FAME derivatization showed an extraordinarily high tolerance of impurities (i.e., pyrolytic products and various extractives). This study also explored the thermal cracking of FAMEs derived from N. oceanica: the thermal cracking of saturated FAMEs was invulnerable at temperatures lower than 400°C. Lastly, this study reported that N. oceanica contained 14.4wt.% of dried N. oceanica and that the introduced methylation technique could be applicable to many research fields sharing the transesterification platform.
authors.affiliation: Department of Environment and Energy at Sejong University, Seoul 05006, South Korea.
authors.forename: Jieun
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Environment and Energy at Sejong University, Seoul 05006, South Korea.
authors.forename: Jong-Min
authors.initials: JM
authors.lastname: Jung
authors.affiliation: Department of Environment and Energy at Sejong University, Seoul 05006, South Korea.
authors.forename: Jechan
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Civil & Environmental Engineering at Hanyang University, Seoul 04763, South Korea.
authors.forename: Ki-Hyun
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Chloland Co. Ltd., 879-2 Gabae-ri, Dongbu-myeon, Geoje, Gyeongsangnam-do, South Korea; Department of Microbiology at Pukyong National University, Busan 48513, South Korea.
authors.forename: Tae O
authors.initials: TO
authors.lastname: Choi
authors.affiliation: Research Institute of Petroleum Technology, Korea Petroleum Quality & Distribution Authority, Cheongju 28115, South Korea.
authors.forename: Jae-Kon
authors.initials: JK
authors.lastname: Kim
authors.affiliation: Department of Microbiology at Pukyong National University, Busan 48513, South Korea. Electronic address: youngjaejeon@pknu.ac.kr.
authors.forename: Young Jae
authors.initials: YJ
authors.lastname: Jeon
authors.affiliation: Department of Environment and Energy at Sejong University, Seoul 05006, South Korea. Electronic address: ekwon74@sejong.ac.kr.
authors.forename: Eilhann E
authors.initials: EE
authors.lastname: Kwon
mesh_terms: D056804:Biofuels; D002384:Catalysis; D004951:Esterification; D004952:Esters; D005227:Fatty Acids / Q000737:chemistry*; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis*; D058009:Stramenopiles / Q000737:chemistry*; D013696:Temperature
chemical_list: D056804:Biofuels; D004952:Esters; D005227:Fatty Acids; D008055:Lipids
keywords: Fatty acid methyl esters; Microalgae; N. oceanica; Pseudo-catalytic transesterification; Thermal cracking