title: Secondary B-cell lymphoma diagnosed by fine-needle aspiration cytology and flow cytometry following penile carcinoma: A case report.
abstract: The number of studies reporting lymphoma as a secondary tumor has gradually increased. However, few studies have reported that occurrence of lymphoma as a secondary tumor following treatment for penile carcinoma, particularly cases in which the lymphoma was diagnosed by fine-needle aspiration cytology and flow cytometry. The present study reports the case of a 62-year-old male patient who was troubled with frequent urination and repeated chest tightness for 5 years. The diagnosis upon admission was penile carcinoma. Two months subsequent to the tumor removal surgery, enlarged lymph nodes were extracted from the patient using fine-needle biopsy, to be analyzed using light microscopy and flow cytometry. Smear results indicated a large number of abnormal cells scattered in the right axillary lymph node. Flow cytometry immunophenotyping of fine-needle aspiration samples indicated the increased expression of cluster of differentiation (CD)79a, CD19, CD20, CD38, κ chain and human leukocyte antigen-DR, which supported a diagnosis of B-cell lymphoma. Thus, the patient was diagnosed with B-cell lymphoma based on the results of the fine-needle aspiration biopsy and flow cytometry. The method of diagnosis and causes of therapy-related leukemia are discussed in the present report.
authors.affiliation: Department of Laboratory Medicine, People's Hospital of Zhejiang Province, Hangzhou, Zhejiang 310014, P.R. China.
authors.forename: Huan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, People's Hospital of Zhejiang Province, Hangzhou, Zhejiang 310014, P.R. China.
authors.forename: Lian-Nv
authors.initials: LN
authors.lastname: Qiu
authors.affiliation: Department of Laboratory Medicine, People's Hospital of Zhejiang Province, Hangzhou, Zhejiang 310014, P.R. China.
authors.forename: Mao
authors.initials: M
authors.lastname: Wu
authors.affiliation: Department of Pathology, People's Hospital of Zhejiang Province, Hangzhou, Zhejiang 310014, P.R. China.
authors.forename: Wan-Yuan
authors.initials: WY
authors.lastname: Chen
authors.affiliation: Department of Urinary Surgery, People's Hospital of Zhejiang Province, Hangzhou, Zhejiang 310014, P.R. China.
authors.forename: Li-Gang
authors.initials: LG
authors.lastname: Ren
authors.affiliation: Department of Pathology, People's Hospital of Zhejiang Province, Hangzhou, Zhejiang 310014, P.R. China.
authors.forename: Xiang-Lei
authors.initials: XL
authors.lastname: He
authors.affiliation: Department of Laboratory Medicine, People's Hospital of Zhejiang Province, Hangzhou, Zhejiang 310014, P.R. China.
authors.forename: Yong-Lie
authors.initials: YL
authors.lastname: Zhou
keywords: fine-needle aspiration biopsy; flow cytometry; lymphoma; penile carcinoma