title: Inducible targeting of cDCs and their subsets in vivo.
abstract: Conventional dendritic cells (cDCs) are essential immune cells linking the innate and adaptive immune system. cDC depletion in mice is an important method to study the function of these cells in vivo. Here we report an inducible in vivo system for cDC depletion in which excision of a loxP flanked Stop signal enables expression of the human diphtheria toxin receptor (DTR) under the control of Zbtb46 (zDC(lSlDTR)). cDCs can be specifically depleted by combining zDC(lSlDTR) mice with a Csf1r(Cre) driver line. In addition, we show that zDC(Cre) mice can be used to produce cDC specific conditional knockout mice (Irf8, Irf4, Notch2) which lack specific subsets of cDCs.
authors.affiliation: Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA. Electronic address: jloschko@rockefeller.edu.
authors.forename: Jakob
authors.initials: J
authors.lastname: Loschko
authors.affiliation: Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA; Rheinische Friedrich-Wilhelms University Bonn, 53113 Bonn, Germany.
authors.forename: Gereon J
authors.initials: GJ
authors.lastname: Rieke
authors.affiliation: Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA.
authors.forename: Heidi A
authors.initials: HA
authors.lastname: Schreiber
authors.affiliation: Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA.
authors.forename: Matthew M
authors.initials: MM
authors.lastname: Meredith
authors.affiliation: Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA.
authors.forename: Kai-Hui
authors.initials: KH
authors.lastname: Yao
authors.affiliation: The laboratory of phagocyte immunobiology, King's College London, UK; Centre for Molecular and Cellular Biology of Inflammation, King's College London, UK; Peter Gorer Department of Immmunobiology, King's College London, UK.
authors.forename: Pierre
authors.initials: P
authors.lastname: Guermonprez
authors.affiliation: Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA; Howard Hughes Medical Institute, USA.
authors.forename: Michel C
authors.initials: MC
authors.lastname: Nussenzweig
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000276:immunology; D003433:Crosses, Genetic; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005434:Flow Cytometry / Q000379:methods; D066257:Heparin-binding EGF-like Growth Factor / Q000276:immunology*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D013154:Spleen / Q000276:immunology
chemical_list: D066257:Heparin-binding EGF-like Growth Factor
keywords: Dendritic cells; In vivo depletion models; zDC