title: Evaluation of recombinant CXCL8(3-73)K11R/G31P in muscle fibrosis and Trichinella larvae encapsulation in a murine model of trichinellosis.
abstract: Trichinella spiralis (T. spiralis) larvae in raw or inadequately cooked meat can cause chronic infections in a wide range of hosts including humans. During the development inside the skeletal muscles, T. spiralis larvae infect muscle cells accompanying with the infiltration of host inflammatory cells, eventually create a new type of cell known as nurse cell developing a surrounding vascular network to support the larvae development. Controlling of host inflammatory responses and angiogenesis influences both the nurse cell differentiation and the parasite larvae development. CXCL8 is a chemokine that acts on G-protein coupled receptors, of which activation contributes to fibrosis and angiogenesis. CXCL8(3-73)K11R/G31P (G31P) has been reported as a CXCL8 analogue. The aim of this study is to investigate the effect of G31P in inflammatory responses and the development of T. spiralis larvae in muscle tissues of mice infected with T. spiralis. The level of inflammatory factors and the morphology of T. spiralis larvae in infected tissues were investigated through ELISA and electron-microscopy analysis. G31P up-regulated IFN-γ and down-regulated CXCL8 level, and impaired the encapsulation of T. spiralis larvae in vivo. The results showed that G31P influenced the development of T. spiralis larvae in muscle tissues.
authors.affiliation: Basic Medical College, Dalian Medical University, Dalian, 116044, China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Yan
authors.affiliation: Basic Medical College, Dalian Medical University, Dalian, 116044, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.affiliation: Basic Medical College, Dalian Medical University, Dalian, 116044, China.
authors.forename: Yuanhua
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Basic Medical College, Dalian Medical University, Dalian, 116044, China.
authors.forename: Yixin
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Basic Medical College, Dalian Medical University, Dalian, 116044, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Basic Medical College, Dalian Medical University, Dalian, 116044, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Dai
authors.affiliation: Basic Medical College, Dalian Medical University, Dalian, 116044, China. Electronic address: maoweifeng@dlmedu.edu.cn.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Mao
authors.affiliation: Basic Medical College, Dalian Medical University, Dalian, 116044, China. Electronic address: dlcuiyu@dlmedu.edu.cn.
authors.forename: Yu
authors.initials: Y
authors.lastname: Cui
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005355:Fibrosis; D006801:Humans; D007371:Interferon-gamma / Q000097:blood / Q000235:genetics; D016209:Interleukin-8 / Q000097:blood* / Q000235:genetics / Q000627:therapeutic use; D007814:Larva; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000648:ultrastructure; D010446:Peptide Fragments / Q000627:therapeutic use*; D017160:Trichinella spiralis / Q000502:physiology*; D014235:Trichinellosis / Q000188:drug therapy*
chemical_list: C000604253:CXCL8(3-72)K11R,G31P, human; D016209:Interleukin-8; D010446:Peptide Fragments; D007371:Interferon-gamma
keywords: CXCL8; G31P; IFN-γ; Trichinella