title: Pregnancy-associated serum N-glycome changes studied by high-throughput MALDI-TOF-MS.
abstract: Pregnancy requires partial suppression of the immune system to ensure maternal-foetal tolerance. Protein glycosylation, and especially terminal sialic acid linkages, are of prime importance in regulating the pro- and anti-inflammatory immune responses. However, little is known about pregnancy-associated changes of the serum N-glycome and sialic acid linkages. Using a combination of recently developed methods, i.e. derivatisation that allows the distinction between α2,3- and α2,6-linked sialic acids by high-throughput MALDI-TOF-MS and software-assisted data processing, we analysed the serum N-glycome of a cohort of 29 healthy women at 6 time points during and after pregnancy. A total of 77 N-glycans were followed over time, confirming in part previous findings while also revealing novel associations (e.g. an increase of FA2BG1S1(6), FA2G1S1(6) and A2BG2S2(6) with delivery). From the individual glycans we calculated 42 derived traits. With these, an increase during pregnancy and decrease after delivery was observed for both α2,3- and α2,6-linked sialylation. Additionally, a difference in the recovery speed after delivery was observed for α2,3- and α2,6-linked sialylation of triantennary glycans. In conclusion, our new high-throughput workflow allowed the identification of novel plasma glycosylation changes with pregnancy.
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center, 2300 RC Leiden, The Netherlands.
authors.forename: Bas C
authors.initials: BC
authors.lastname: Jansen
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center, 2300 RC Leiden, The Netherlands.|Department of Rheumatology, Erasmus University Medical Center, 3000 CA Rotterdam, The Netherlands.|Department of Rheumatology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands.
authors.forename: Albert
authors.initials: A
authors.lastname: Bondt
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center, 2300 RC Leiden, The Netherlands.
authors.forename: Karli R
authors.initials: KR
authors.lastname: Reiding
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center, 2300 RC Leiden, The Netherlands.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Lonardi
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center, 2300 RC Leiden, The Netherlands.
authors.forename: Coen J
authors.initials: CJ
authors.lastname: de Jong
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center, 2300 RC Leiden, The Netherlands.
authors.forename: David
authors.initials: D
authors.lastname: Falck
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center, 2300 RC Leiden, The Netherlands.
authors.forename: Guinevere S M
authors.initials: GS
authors.lastname: Kammeijer
authors.affiliation: Department of Rheumatology, Erasmus University Medical Center, 3000 CA Rotterdam, The Netherlands.
authors.forename: Radboud J E M
authors.initials: RJ
authors.lastname: Dolhain
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center, 2300 RC Leiden, The Netherlands.|Department of Rheumatology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands.|Univ. Lille, CNRS, UMR 8576, UGSF, Unité de Glycobiologie Structurale et Fonctionnelle, F 59 000 Lille, France.
authors.forename: Yoann
authors.initials: Y
authors.lastname: Rombouts
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center, 2300 RC Leiden, The Netherlands.
authors.forename: Manfred
authors.initials: M
authors.lastname: Wuhrer
mesh_terms: D001330:Electronic Data Processing; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D011134:Polysaccharides / Q000032:analysis*; D011247:Pregnancy; D044967:Serum / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
chemical_list: D011134:Polysaccharides