title: Rare natural type 3/type 2 intertypic capsid recombinant vaccine-related poliovirus isolated from a case of acute flaccid paralysis in Brazil, 2015.
abstract: A natural type 3/type 2 intertypic capsid recombinant vaccine-related poliovirus was isolated from an acute flaccid paralytic case in Brazil. Genome sequencing revealed the uncommon location of the crossover site in the VP1 coding region (nucleotides 3251-3258 of Sabin 3 genome). The Sabin 2 donor sequence replaced the last 118 nt of VP1, resulting in the substitution of the complete antigenic site IIIa by PV2-specific amino acids. The low overall number of nucleotide substitutions in P1 region indicated that the predicted replication time of the isolate was about 8-9 weeks. Two of the principal determinants of attenuation in Sabin 3 genomes were mutated (U472C and C2493U), but the temperature-sensitive phenotype of the isolate was preserved. Our results support the theory that there exists a PV3/PV2 recombination hotspot site in the tail region of the VP1 capsid protein and that the recombination may occur soon after oral poliovirus vaccine administration.
authors.affiliation: Enterovirus Laboratory, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
authors.forename: Klécia M S M
authors.initials: KMSM
authors.lastname: Cassemiro
authors.affiliation: Enterovirus Laboratory, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
authors.forename: Fernanda M
authors.initials: FM
authors.lastname: Burlandy
authors.affiliation: Enterovirus Laboratory, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
authors.forename: Edson E
authors.initials: EE
authors.lastname: da Silva
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001938:Brazil; D002213:Capsid / Q000276:immunology; D036022:Capsid Proteins / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007223:Infant; D008297:Male; D011051:Poliomyelitis / Q000821:virology*; D017955:Poliovirus / Q000145:classification / Q000235:genetics* / Q000276:immunology; D011055:Poliovirus Vaccine, Oral / Q000009:adverse effects* / Q000276:immunology; D011782:Quadriplegia / Q000821:virology*; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D014614:Vaccines, Synthetic / Q000009:adverse effects* / Q000276:immunology
chemical_list: D036022:Capsid Proteins; D011055:Poliovirus Vaccine, Oral; C442540:VP1 protein, Poliovirus; D014614:Vaccines, Synthetic