title: Development of a novel protein chip for the detection of bluetongue virus in China.
abstract: Bluetongue (BT), which is caused by the BT virus (BTV), is an important disease in ruminants that leads to significant economic losses in the husbandry industry. To detect BTV-specific antibodies in serum, a protein chip detection method based on a novel solid supporting material known as polymer-coated initiator-integrated poly (dimethyl siloxane) (iPDMS) was developed. With a threshold of 25% (signal-to-noise percentage), the sensitivity and specificity of the protein chip were 98.6% and 94.8%, respectively. Furthermore, spot serum samples obtained from six provinces of China were tested with the protein chip and a commercially available BTV enzyme-linked immunosorbent assay (ELISA) kit (IDEXX). Of 615 samples, BTV-specific antibodies were detected in 200 (32.52%) by the protein chip and in 176 (28.62%) by the IDEXX BTV ELISA kit. Comparison of the protein chip with the commercial IDEXX BTV ELISA kit yielded the following spot serum detection results: a total coincidence, a negative coincidence and a positive coincidence of 95.12%, 99.28% and 86.5%, respectively. With the protein chip, the BTV-specific serum antibody was detected in samples from all six provinces, and the positive rates ranged from 4.12 to 74.4%. These results indicate that this protein chip detection method based on iPDMS is useful for the serological diagnosis of BTV infection and for epidemiological investigation.
authors.affiliation: The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. Electronic address: x_qingyuan@163.com.
authors.forename: Q Y
authors.initials: QY
authors.lastname: Xu
authors.affiliation: The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. Electronic address: ecsunhvri@163.com.
authors.forename: E C
authors.initials: EC
authors.lastname: Sun
authors.affiliation: Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China. Electronic address: fyf_anna@163.com.
authors.forename: Y F
authors.initials: YF
authors.lastname: Feng
authors.affiliation: The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. Electronic address: lijunping916@163.com.
authors.forename: J P
authors.initials: JP
authors.lastname: Li
authors.affiliation: The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. Electronic address: shuanglv8728@163.com.
authors.forename: S
authors.initials: S
authors.lastname: Lv
authors.affiliation: The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. Electronic address: 472764575@qq.com.
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. Electronic address: 403584095@qq.com.
authors.forename: H X
authors.initials: HX
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. Electronic address: 15776628009@163.com.
authors.forename: J K
authors.initials: JK
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. Electronic address: dlwu@hvri.ac.cn.
authors.forename: D L
authors.initials: DL
authors.lastname: Wu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001819:Bluetongue / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D001820:Bluetongue virus / Q000235:genetics / Q000276:immunology*; D002417:Cattle / Q000821:virology; D002418:Cattle Diseases / Q000175:diagnosis / Q000821:virology; D002681:China / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D012680:Sensitivity and Specificity; D012756:Sheep / Q000821:virology
chemical_list: D000914:Antibodies, Viral
keywords: Bluetongue; Epidemiological investigation; Monoclonal antibody; Protein chip