title: Role of S-methylisothiourea (SMT) in renal ischemia/reperfusion injury in rats.
abstract: BACKGROUND
  Excessive production of nitric oxide (NO) via inducible nitric oxide synthase (iNOS) is associated in renal ischemia reperfusion injury (IRI).
  
  
  OBJECTIVE
  This study was designed to investigate the role of S-methylisothiourea (SMT) as selective inhibitor iNOS in renal IRI.
  
  
  METHODS
  Male Wistar rats were subjected to 45 minutes of bilateral renal ischemia by occlusion of renal vessels of both kidney followed by 24 hours of reperfusion. Prior to renal IRI, the rats received either vehicle (saline, group 2) or SMT (50 mg/kg, group 3), and were compared with the sham-operated animals (group 1). At the end of reperfusion period, the rats were sacrificed for kidney tissue pathology investigation.
  
  
  RESULTS
  Serum creatinine (Cr), blood urea nitrogen (BUN), nitrite levels, and kidney weight significantly increased in groups 2 and 3 (P < 0.05). Kidney tissue damage scores in groups 2 and 3 were also higher than that in the sham-operated group (P < 0.05).
  
  
  CONCLUSIONS
  SMT not only prevent the kidney during IRI, but also promotes kidney function disturbance and severity of renal injury.
authors.affiliation: Water & Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Kanani
authors.affiliation: Water & Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Faezeh
authors.initials: F
authors.lastname: Fazelnia
authors.affiliation: Water & Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Mohaddeseh
authors.initials: M
authors.lastname: Mojarradfard
authors.affiliation: Water & Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran ; Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran ; Isfahan MN Institute of Basic & Applied Sciences Research, Isfahan, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Nematbakhsh
authors.affiliation: Water & Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Moslemi
authors.affiliation: Water & Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Eshraghi-Jazi
authors.affiliation: Department of Clinical Pathology, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Ardeshir
authors.initials: A
authors.lastname: Talebi
keywords: Nitric oxide; Renal injury; Renal ischemia reperfusion; S-methylisothiourea