title: Studies of silicon nanoparticles uptake and biodegradation in cancer cells by Raman spectroscopy.
abstract: In-vitro Raman micro-spectroscopy was used for diagnostics of the processes of uptake and biodegradation of porous silicon nanoparticles (SiNPs) in breast cancer cells (MCF-7 cell line). Two types of nanoparticles, with and without photoluminescence in the visible spectral range, were investigated. The spatial distribution of photoluminescent SiNPs within the cells obtained by Raman imaging was verified by high-resolution structured-illumination optical microscopy. Nearly complete biodegradation of SiNPs inside the living cells was observed after 13days of the incubation. The results reveal new prospects of multi-modal visualization of SiNPs inside cancer cells for theranostic applications.
authors.affiliation: Leibniz Institute of Photonic Technology, Jena, Germany; Institute of Physical Chemistry, Abbe Center of Photonics, Friedrich-Schiller-University, Jena Germany. Electronic address: Elen.tolstik@ipht-jena.de.
authors.forename: Elen
authors.initials: E
authors.lastname: Tolstik
authors.affiliation: Lomonosov Moscow State University, Physics Department, Moscow, Russia; National Research Nuclear University MEPhI, Moscow, Russia.
authors.forename: Liubov Andreevna
authors.initials: LA
authors.lastname: Osminkina
authors.affiliation: Leibniz Institute of Photonic Technology, Jena, Germany; Institute of Physical Chemistry, Abbe Center of Photonics, Friedrich-Schiller-University, Jena Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Matthäus
authors.affiliation: Leibniz Institute of Photonic Technology, Jena, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Burkhardt
authors.affiliation: Lomonosov Moscow State University, Physics Department, Moscow, Russia.
authors.forename: Konstantine Eduardovich
authors.initials: KE
authors.lastname: Tsurikov
authors.affiliation: Lomonosov Moscow State University, Physics Department, Moscow, Russia.
authors.forename: Uliana Alexandrovna
authors.initials: UA
authors.lastname: Natashina
authors.affiliation: Lomonosov Moscow State University, Physics Department, Moscow, Russia; National Research Nuclear University MEPhI, Moscow, Russia.
authors.forename: Victor Yurievich
authors.initials: VY
authors.lastname: Timoshenko
authors.affiliation: Leibniz Institute of Photonic Technology, Jena, Germany; Institute of Physical Chemistry, Abbe Center of Photonics, Friedrich-Schiller-University, Jena Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Heintzmann
authors.affiliation: Leibniz Institute of Photonic Technology, Jena, Germany; Institute of Physical Chemistry, Abbe Center of Photonics, Friedrich-Schiller-University, Jena Germany.
authors.forename: Juergen
authors.initials: J
authors.lastname: Popp
authors.affiliation: Leibniz Institute of Photonic Technology, Jena, Germany.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Sivakov
mesh_terms: D006801:Humans; D061986:MCF-7 Cells; D053758:Nanoparticles; D061848:Optical Imaging / Q000379:methods; D016062:Porosity; D012825:Silicon / Q000493:pharmacokinetics*; D012822:Silicon Dioxide; D013059:Spectrum Analysis, Raman
chemical_list: D012822:Silicon Dioxide; D012825:Silicon
keywords: Biodegradation; Cancer theranostic; HR-SIM microscopy; Raman spectroscopy; Silicon nanoparticles