title: Dual Regulation of Cell Death and Cell Survival upon Induction of Cellular Stress by Isopimara-7,15-Dien-19-Oic Acid in Cervical Cancer, HeLa Cells In vitro.
abstract: The Fritillaria imperialis is an ornamental flower that can be found in various parts of the world including Iraq, Afghanistan, Pakistan, and the Himalayas. The use of this plant as traditional remedy is widely known. This study aims to unveil the anti-cancer potentials of Isopimara-7,15-Dien-19-Oic Acid, extracted from the bulbs of F. imperialis in cervical cancer cell line, HeLa cells. Flow cytometry analysis of cell death, gene expression analysis via cDNA microarray and protein array were performed. Based on the results, Isopimara-7,15-Dien-19-Oic acid simultaneously induced cell death and promoted cell survival. The execution of apoptosis was apparent based on the flow cytometry results and regulation of both pro and anti-apoptotic genes. Additionally, the regulation of anti-oxidant genes were up-regulated especially thioredoxin, glutathione and superoxide dismutase- related genes. Moreover, the treatment also induced the activation of pro-survival heat shock proteins. Collectively, Isopimara-7,15-Dien-19-Oic Acid managed to induce cellular stress in HeLa cells and activate several anti- and pro survival pathways.
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia Serdang, Malaysia.
authors.forename: Nadiah
authors.initials: N
authors.lastname: Abu
authors.affiliation: Laboratory of Immunotherapeutics and Vaccine (LIVES), Institute of Bioscience, Universiti Putra Malaysia Serdang, Malaysia.
authors.forename: Swee K
authors.initials: SK
authors.lastname: Yeap
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia Serdang, Malaysia.
authors.forename: Ahmad Z Mat
authors.initials: AZ
authors.lastname: Pauzi
authors.affiliation: Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang Kuantan, Malaysia.
authors.forename: M Nadeem
authors.initials: MN
authors.lastname: Akhtar
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia Serdang, Malaysia.
authors.forename: Nur R
authors.initials: NR
authors.lastname: Zamberi
authors.affiliation: Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang Kuantan, Malaysia.
authors.forename: Jamil
authors.initials: J
authors.lastname: Ismail
authors.affiliation: Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang Kuantan, Malaysia.
authors.forename: Seema
authors.initials: S
authors.lastname: Zareen
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia Serdang, Malaysia.
authors.forename: Noorjahan B
authors.initials: NB
authors.lastname: Alitheen
keywords: 15-dien-19-oic acid; Fritillaria imperialis; HeLa; antioxidant; isopimara-7