title: Association between lipoxin A4 and interleukin-12 in gingival crevicular fluid: a preliminary investigation.
abstract: OBJECTIVE
  The balance between host proinflammatory and anti-inflammatory immune responses is a key determinant for the pathogenesis of periodontal disease. This study aimed to explore the possibility of an association between lipoxin A4 and interleukin-12 in chronic periodontitis.
  
  
  METHODS
  Forty-five patients with chronic periodontitis and 45 periodontally healthy patients were included in this case-control study. Plaque index, calculus index, gingival index, sulcus bleeding index, full-mouth probing depth and clinical attachment loss were recorded. Gingival crevicular fluid samples were collected and analysed for interleukin-12 and lipoxin A4 using ELISA.
  
  
  RESULTS
  The mean concentration of lipoxin A4 was lower in the periodontitis group compared with the periodontally healthy group. There was a negative correlation between interleukin-12 and lipoxin A4 in both groups. There was a negative correlation between clinical attachment loss and lipoxin A4, and a positive correlation between clinical attachment loss and interleukin-12. However, the correlations were statistically insignificant.
  
  
  CONCLUSIONS
  The mean interleukin-12 concentration was significantly higher in gingival crevicular fluid from patients with periodontitis than in that from with healthy patients, and the mean lipoxin A4 concentration was lower in patients with periodontitis than in healthy patients. Lipoxin A4 possibly has an inhibitory effect on interleukin-12.
authors.affiliation: Department of Periodontics, M.R. Ambedkar Dental College and Hospital, Bangalore, India.
authors.forename: F
authors.initials: F
authors.lastname: Tarannum
authors.affiliation: Department of Periodontics, M.R. Ambedkar Dental College and Hospital, Bangalore, India.
authors.forename: M
authors.initials: M
authors.lastname: Faizuddin
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000378:metabolism; D003774:Dental Plaque Index; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D018664:Interleukin-12 / Q000032:analysis*; D044045:Lipoxins / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D010512:Periodontal Index
chemical_list: D044045:Lipoxins; C040527:lipoxin A4; D018664:Interleukin-12
keywords: chronic periodontitis; gingival crevicular fluid; inflammation; interleukin-12; lipoxins; resolution