title: Inhibition of PDGF-induced migration and TNF-α-induced ICAM-1 expression by maltotetraose from bamboo stem extract (BSE) in mouse vascular smooth muscle cells.
abstract: Expression of intercellular adhesion molecule-1 (ICAM-1) on vascular smooth muscle cells (VSMCs) plays an important role in the progression of atherosclerosis. We investigated the effects of bamboo stem extract (BSE) on motility and ICAM-1 expression by using mouse MOVAS-1 cells. Active constituents of BSE exhibiting an inhibitory activity on TNF-α-induced ICAM1 expression were identified using HPLC.
  
  
  
  The effects of BSE on platelet-derived growth factor (PDGF)-BB-induced migration, tumor necrosis factor alpha (TNF-α)-induced expression of ICAM-1, and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) activation were investigated. BSE inhibited migration of MOVAS-1 cells and sprout formation by mouse aorta explants. Reverse transcription PCR analysis and promoter reporter assays revealed that BSE suppressed ICAM-1 expression by inhibiting NF-κB activity. In addition, BSE reduced adhesion between VSMCs and monocytes. Several oligosaccharides were identified in BSE. Among the oligosaccharides contained in BSE, maltotetraose and stachyose were potent inhibitors of TNF-α-induced ICAM-1 expression. We confirmed that maltotetraose reduced PDGF-induced sprout formation by mouse aorta explants and inhibited TNF-α-induced NF-κB activation and ICAM-1 expression in MOVAS-1 cells.
  
  
  
  The BSE constituent maltotetraose may be beneficial in the suppression of early atherosclerosis development and could be developed as a dietary supplement for cardiovascular health.
authors.affiliation: Department of Biological Sciences, College of Biological Science and Biotechnology, Konkuk University, Seoul, Republic of Korea.|Cancer and Metabolism Institute, Konkuk University, Seoul, Republic of Korea.
authors.forename: Soon Young
authors.initials: SY
authors.lastname: Shin
authors.affiliation: Cancer and Metabolism Institute, Konkuk University, Seoul, Republic of Korea.
authors.forename: You Jung
authors.initials: YJ
authors.lastname: Jung
authors.affiliation: Division of Bioscience and Biotechnology, BMIC, Konkuk University, Seoul, Republic of Korea.
authors.forename: Yeonjoong
authors.initials: Y
authors.lastname: Yong
authors.affiliation: Division of Bioscience and Biotechnology, BMIC, Konkuk University, Seoul, Republic of Korea.
authors.forename: Hi Jae
authors.initials: HJ
authors.lastname: Cho
authors.affiliation: Division of Bioscience and Biotechnology, BMIC, Konkuk University, Seoul, Republic of Korea.
authors.forename: Yoongho
authors.initials: Y
authors.lastname: Lim
authors.affiliation: Department of Biological Sciences, College of Biological Science and Biotechnology, Konkuk University, Seoul, Republic of Korea. yhlee58@konkuk.ac.kr.|Cancer and Metabolism Institute, Konkuk University, Seoul, Republic of Korea. yhlee58@konkuk.ac.kr.
authors.forename: Young Han
authors.initials: YH
authors.lastname: Lee
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D008297:Male; D008320:Maltose / Q000031:analogs & derivatives* / Q000494:pharmacology; D008807:Mice, Inbred BALB C; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D031787:Sasa / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism / Q000494:pharmacology*
chemical_list: C090509:Icam1 protein, mouse; D016328:NF-kappa B; D010936:Plant Extracts; D010982:Platelet-Derived Growth Factor; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D008320:Maltose; C009819:maltotetraose
keywords: Atherosclerosis; Bamboo stem extract; Platelet-derived growth factor; Vascular smooth muscle cells; intercellular adhesion molecule-1