title: [microRNA-181b promotes migration and invasion of osteosarcoma cells by targeting N-myc downstream regulated gene 2].
abstract: OBJECTIVE
  To investigate the effects of miR-181b on the migration and invasion of osteosarcoma cells.
  
  
  METHODS
  Three cultured osteosarcoma cell lines and MG-63 cells transfected with miR-181b inhibitor were examined for miR-181b expression using qRT-PCR analysis. The cell migration and invasion of the transfected cells were assessed with Transwell assay. The targets of miR-181b were predicted using a miRNA target prediction software and the results were verified with luciferase reporter assay. The target protein expression in osteosarcoma cells lines was determined by Western blotting, and the cell migration and invasion changes following inhibition of miR-181b or its target protein were assessed using Transwell assay.
  
  
  RESULTS
  All the 3 osteosarcoma cells lines showed significantly up-regulated miR-181b expression. Inhibition of miR-181b expression obviously suppressed the migration and invasion of MG-63 cells. Based on luciferase reporter assay, N-myc downstream regulated gene 2 (NDRG2) was identified as the direct target gene of miR-181b, and inhibition of NDRG2 expression significantly reversed the effect of miR-181b on cell migration and invasion in MG-63 cells.
  
  
  CONCLUSIONS
  miR-181b is over-expressed in osteosarcoma cells, and inhibition of miR-181b, which directly targets NDRG2, can suppress the migration and invasion of osteosarcoma cells.
authors.affiliation: Department of Orthopedics and Traumatology, First Affiliated Hospital of Jinan University, Guangzhou 510630, China.E-mail: tzjl@jnu.edu.cn.
authors.forename: Jian-Li
authors.initials: JL
authors.lastname: Shao
authors.forename: Zhi-Zhong
authors.initials: ZZ
authors.lastname: Li
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.forename: Gen-Long
authors.initials: GL
authors.lastname: Jiao
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Zhou
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Sun
mesh_terms: D001859:Bone Neoplasms / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D012516:Osteosarcoma / Q000235:genetics / Q000473:pathology*; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C540193:MIrn181 microRNA, human; D035683:MicroRNAs; C466228:NDRG2 protein, human; D025521:Tumor Suppressor Proteins