title: Anti-infective mannose receptor immune mechanism in large yellow croaker (Larimichthys crocea).
abstract: Mannose receptor (MR) is a pattern recognition receptor (PRR) that plays a significant role in immunity responses. Its role has been described extensively in mammals, but very rarely in fish. Recently, with the rapid development of an aquaculture industry cultivating large yellow croaker (Larimichthys crocea), infectious diseases caused by viruses, bacteria and parasites are becoming more frequent and more severe, in particular bacterial infections caused by Vibrio anguillarum, resulting in great economical losses. Extensive use of antibiotics as conventional treatment has led to microenvironment imbalances, development of drug-resistant bacteria and deposition of drug residues, which cause environmental pollution and ultimately affect human health. The purpose of this pilot study was to detect the transcriptional levels of C-type mannose receptor genes MRC1 (4710-bp ORF; encoding 1437 aa; a signal peptide, a SMART RICIN domain, a SMART FN2 domain, eight SMART CLECT domain, and a transmembrane helix region) and MRC2 (3996-bp ORF; encoding 1484 aa; a SMART FN2 domain, eight SMART CLECT domains, and a transmembrane region) in the liver, kidney and spleen tissues of L. crocea challenged by V. anguillarum, to explore the effective domain and the molecular response mechanisms of MRC1 and MRC2, and, ultimately, to explore the possibility of developing a vaccine targeting V. anguillarum infections.
authors.affiliation: National Engineering Research Center of Maricultural Facilities of China, College of Marine Science and Technology, Zhejiang Ocean University, Haida South Road 1, 316022, Zhoushan, Zhejiang, PR China.
authors.forename: Xiangli
authors.initials: X
authors.lastname: Dong
authors.affiliation: National Engineering Research Center of Maricultural Facilities of China, College of Marine Science and Technology, Zhejiang Ocean University, Haida South Road 1, 316022, Zhoushan, Zhejiang, PR China; Università degli Studi di Napoli "Federico II", Parco Gussone 1, 80055, Portici, Naples, Italy; Enea CR Portici, P. le E. Fermi, 1, 80055, Portici, Naples, Italy.
authors.forename: Jiji
authors.initials: J
authors.lastname: Li
authors.affiliation: National Engineering Research Center of Maricultural Facilities of China, College of Marine Science and Technology, Zhejiang Ocean University, Haida South Road 1, 316022, Zhoushan, Zhejiang, PR China.
authors.forename: Jianyu
authors.initials: J
authors.lastname: He
authors.affiliation: National Engineering Research Center of Maricultural Facilities of China, College of Marine Science and Technology, Zhejiang Ocean University, Haida South Road 1, 316022, Zhoushan, Zhejiang, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: National Engineering Research Center of Maricultural Facilities of China, College of Marine Science and Technology, Zhejiang Ocean University, Haida South Road 1, 316022, Zhoushan, Zhejiang, PR China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Jiang
authors.affiliation: National Engineering Research Center of Maricultural Facilities of China, College of Marine Science and Technology, Zhejiang Ocean University, Haida South Road 1, 316022, Zhoushan, Zhejiang, PR China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Ye
authors.affiliation: National Engineering Research Center of Maricultural Facilities of China, College of Marine Science and Technology, Zhejiang Ocean University, Haida South Road 1, 316022, Zhoushan, Zhejiang, PR China. Electronic address: wucw2013@126.com.
authors.forename: Changwen
authors.initials: C
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005393:Fish Diseases / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007113:Immunity, Innate; D037181:Lectins, C-Type / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010473:Perciformes; D010802:Phylogeny; D000072417:Protein Domains; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment / Q000662:veterinary; D014733:Vibrio / Q000502:physiology; D014735:Vibrio Infections / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface
keywords: Expression analysis; Immune mechanism; Larimichthys crocea; Mannose receptor; Molecular characterization