title: Distinct metabolic effects of resveratrol on lipogenesis markers in mice adipose tissue treated with high-polyunsaturated fat and high-protein diets.
abstract: OBJECTIVE
  A healthy diet is essential for the prevention of metabolic syndrome. The present study evaluated the effect of resveratrol associated with high-polyunsaturated fat and high-protein diets on expression of adipogenic and lipogenic genes.
  
  
  METHODS
  FVB/N mice were divided into 6 groups (n=7 each) and fed with experimental diets for 60days: standard (ST), high-fat diet (HFD), and high-protein diet (HPD), with and without resveratrol (RSV) (4g/kg diet). The body weight, food intake, energy intake (kcal), and blood parameters (HDL-C, total cholesterol, glucose, and triglyceride levels) were assessed. Real-time PCR was performed to analyze the expression of adipogenesis and lipogenesis markers: PPARγ, SREBP-1c, ACC and FAS in samples from perigonadal adipose tissue.
  
  
  RESULTS
  In the HPD+RSV group, resveratrol decreased body weight, body adiposity, adipose tissue weight, adipocyte area, total cholesterol, ACC and FAS expression, and increased HDL-cholesterol in comparison to HPD. In the HPD group there was a decrease in adipocyte area, as well as PPARγ, SREBP-1c and ACC expression in comparison to ST. While in HFD+RSV, resveratrol decreased levels of total cholesterol in comparison to HFD. In the HFD group there was decrease in body weight, and PPARγ, SREBP-1c and ACC expression in comparison to ST.
  
  
  CONCLUSIONS
  The obtained results show that resveratrol decreases lipogenesis markers and metabolic parameters in the setting of a high-protein diet. Moreover, resveratrol decreased total cholesterol in both diets. These results point to the increased potential of resveratrol use in prevention of metabolic syndrome, acting on different dietary compositions.
authors.affiliation: Laboratory of Health Science, Postgraduate Program in Health Science, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, Minas Gerais, Brazil; Instituto Federal de Minas Gerais (IFMG), São João Evangelista, Minas Gerais, Brazil.
authors.forename: Keila Lopes
authors.initials: KL
authors.lastname: Mendes
authors.affiliation: Laboratory of Health Science, Postgraduate Program in Health Science, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, Minas Gerais, Brazil; Universidade Estadual de Montes Claros (Unimontes), Montes Claros, Minas Gerais, Brazil.
authors.forename: Lucinéia
authors.initials: L
authors.lastname: de Pinho
authors.affiliation: Laboratory of Health Science, Postgraduate Program in Health Science, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, Minas Gerais, Brazil.
authors.forename: João Marcus Oliveira
authors.initials: JM
authors.lastname: Andrade
authors.affiliation: Laboratory of Health Science, Postgraduate Program in Health Science, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, Minas Gerais, Brazil.
authors.forename: Alanna Fernandes
authors.initials: AF
authors.lastname: Paraíso
authors.affiliation: Laboratory of Health Science, Postgraduate Program in Health Science, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, Minas Gerais, Brazil.
authors.forename: Jamille Fernandes
authors.initials: JF
authors.lastname: Lula
authors.affiliation: Laboratory of Health Science, Postgraduate Program in Health Science, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, Minas Gerais, Brazil.
authors.forename: Simone Moreira
authors.initials: SM
authors.lastname: Macedo
authors.affiliation: Texas Tech University, Lubbock, TX, United States.
authors.forename: John David
authors.initials: JD
authors.lastname: Feltenberger
authors.affiliation: Laboratory of Health Science, Postgraduate Program in Health Science, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, Minas Gerais, Brazil.
authors.forename: André Luiz Sena
authors.initials: AL
authors.lastname: Guimarães
authors.affiliation: Laboratory of Health Science, Postgraduate Program in Health Science, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, Minas Gerais, Brazil.
authors.forename: Alfredo Maurício Batista
authors.initials: AM
authors.lastname: de Paula
authors.affiliation: Laboratory of Health Science, Postgraduate Program in Health Science, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, Minas Gerais, Brazil; Institute of Agrarian Sciences (ICA), Food Engineering Department, Universidade Federal de Minas Gerais (UFMG), Montes Claros, Minas Gerais, Brazil. Electronic address: sergiosousas@hotmail.com.
authors.forename: Sérgio Henrique Sousa
authors.initials: SH
authors.lastname: Santos
mesh_terms: D050156:Adipogenesis / Q000235:genetics; D000273:Adipose Tissue / Q000187:drug effects*; D000818:Animals; D001823:Body Composition; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D004044:Dietary Proteins / Q000008:administration & dosage*; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage*; D005260:Female; D050155:Lipogenesis / Q000187:drug effects*; D051379:Mice; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D004042:Dietary Fats, Unsaturated; D004044:Dietary Proteins; D005231:Fatty Acids, Unsaturated; D013267:Stilbenes; D000077185:Resveratrol
keywords: Adipogenesis; Cholesterol; Diet; Lipogenesis; Resveratrol; Resveratrol (PubChem CID: 445154)