title: Chemico-mechanical imaging of Barrett's oesophagus.
abstract: Barrett's oesophagus is a condition characterized by a change in the lining of the oesophagus that markedly increases the risk of adenocarcinoma. We demonstrate the first site-matched application of Brillouin microscopy, Raman microscopy and FTIR micro-spectroscopic imaging to ex-vivo epithelial tissue - Barrett's oesophagus. The mechanical and chemical characters of the epithelium were assessed in histological sections from a patient subjected to endoscopic oesophageal biopsy. Previous studies have shown that both these properties change within the oesophageal wall, owing to the presence of distinct cellular and extracellular constituents which are putatively affected by oesophageal cancer. Brillouin microscopy enables maps of elasticity of the epithelium to be obtained, whilst Raman and FTIR imaging provide 'chemical images' without the need for labelling or staining. This site-matched approach provides a valuable platform for investigating the structure, biomechanics and composition of complex heterogeneous systems. A combined Brillouin-Raman device has potential for in-vivo diagnosis of pathology. First application of site-matched micro Brillouin, Raman and FTIR spectroscopic imaging to epithelial tissue in Barrett's oesophagus.
authors.affiliation: University of Exeter, School of Physics, Exeter, EX4 4QL, UK. f.palombo@exeter.ac.uk.
authors.forename: Francesca
authors.initials: F
authors.lastname: Palombo
authors.affiliation: University of Perugia, Department of Physics and Geology, Perugia, I-06100, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Madami
authors.affiliation: University of Perugia, Department of Physics and Geology, Perugia, I-06100, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Fioretto
authors.affiliation: University of Exeter, School of Physics, Exeter, EX4 4QL, UK.
authors.forename: Jayakrupakar
authors.initials: J
authors.lastname: Nallala
authors.affiliation: Gloucestershire Royal Hospital, Gloucester, GL1 3NN, UK.
authors.forename: Hugh
authors.initials: H
authors.lastname: Barr
authors.affiliation: University of Exeter, School of Physics, Exeter, EX4 4QL, UK.|ENSEIRB-MATMECA Telecommunications Department, Bordeaux, F-33402, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: David
authors.affiliation: University of Exeter, School of Physics, Exeter, EX4 4QL, UK. n.stone@exeter.ac.uk.
authors.forename: Nick
authors.initials: N
authors.lastname: Stone
mesh_terms: D000230:Adenocarcinoma; D001471:Barrett Esophagus / Q000000981:diagnostic imaging*; D004848:Epithelium / Q000000981:diagnostic imaging*; D004938:Esophageal Neoplasms; D004945:Esophagoscopy; D006801:Humans; D008853:Microscopy / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman
keywords: Brillouin; FTIR spectroscopy; Raman; biomechanics; epithelial tissue; light scattering