title: Simultaneous determination of the main amino thiol and thione in human whole blood by CE and LC.
abstract: BACKGROUND
  Two precolumn fluorescence derivatization procedures by two different sulfhydryl-reactive iodoacetyl reagents were established to measure simultaneously glutathione and l-ergothioneine in human whole blood by means of CE and LC.
  
  
  METHODS
  Separations were achieved in <5 min on a reverse-phase column (100 mm × 4.6 mm Zorbax Eclipse Plus C18 3.5 µm) for LC analysis, and on an uncoated fused-silica capillary (60 cm × 50 µm) for CE analysis, monitoring the fluorescence of derivatives.
  
  
  RESULTS
  Performance of the assays was good in terms of linearity, recovery, intra- and inter-day precision and LOD and LOQ.
  
  
  CONCLUSIONS
  This novel approach allows rapid assessment of circulating glutathione and l-ergothioneine concentrations for clinical and research purposes.
authors.affiliation: Department of Biomedical Sciences, School of Medicine, University of Sassari, Sassari, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Sotgia
authors.affiliation: Department of Biomedical Sciences, School of Medicine, University of Sassari, Sassari, Italy.
authors.forename: Dionigia
authors.initials: D
authors.lastname: Arru
authors.affiliation: Department of Biomedical Sciences, School of Medicine, University of Sassari, Sassari, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Sotgiu
authors.affiliation: Department of Clinical Pharmacology, School of Medicine, Flinders University, Adelaide, Australia.
authors.forename: Arduino A
authors.initials: AA
authors.lastname: Mangoni
authors.affiliation: Department of Biomedical Sciences, School of Medicine, University of Sassari, Sassari, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Forteschi
authors.affiliation: Department of Biomedical Sciences, School of Medicine, University of Sassari, Sassari, Italy.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Pintus
authors.affiliation: Department of Biomedical Sciences, School of Medicine, University of Sassari, Sassari, Italy.|Quality Control Unit, University Hospital Sassari (AOU), Sassari, Italy.
authors.forename: Ciriaco
authors.initials: C
authors.lastname: Carru
authors.affiliation: Department of Biomedical Sciences, School of Medicine, University of Sassari, Sassari, Italy.
authors.forename: Angelo
authors.initials: A
authors.lastname: Zinellu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000975:Antioxidants / Q000493:pharmacokinetics; D002853:Chromatography, Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D004880:Ergothioneine / Q000097:blood*; D005260:Female; D005978:Glutathione / Q000097:blood*; D006801:Humans; D057230:Limit of Detection; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results
chemical_list: D000975:Antioxidants; D004880:Ergothioneine; D005978:Glutathione
keywords: LC; amino thione; antioxidant; assay; biomarkers; capillary electrophoresis; derivatization; fluorescence; low-molecular weight thiols; method development