title: Hop (Humulus lupulus L.) response mechanisms in drought stress: Proteomic analysis with physiology.
abstract: Drought is one of the major environmental devastating stressors that impair the growth and productivity of crop plants. Despite the relevance of drought stress, changes in physiology and resistance mechanisms are not completely understood for certain crops, including hop (Humulus lupulus L.). In this research the drought response of hop was studied using a conventional physiological approach (gas exchange techniques, fluorescence, relative water content measurements) and proteomic analysis (2D-DIGE). Plants of two cultivars (Aurora and Savinjski golding) were exposed to progressive drought in a pot experiment and analysed at different stress stages (mild, moderate and severe). Measurements of relative water content revealed a hydrostable water balance of hop. Photosynthesis was decreased due to stomatal and non-stomatal limitation to the same extent in both cultivars. Of 28 identified differentially abundant proteins, the majority were down regulated and included in photosynthetic (41%) and sugar metabolism (33%). Fifteen % of identified proteins were classified into the nitrogen metabolism, 4% were related to a ROS related pathway and 7% to other functions.
authors.affiliation: Slovenian Institute of Hop Research and Brewing, Cesta Žalskega tabora 2, SI-3310, Žalec, Slovenia.
authors.forename: Zala
authors.initials: Z
authors.lastname: Kolenc
authors.affiliation: Agronomy Department, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000, Ljubljana, Slovenia.
authors.forename: Dominik
authors.initials: D
authors.lastname: Vodnik
authors.affiliation: Agronomy Department, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000, Ljubljana, Slovenia.
authors.forename: Stanislav
authors.initials: S
authors.lastname: Mandelc
authors.affiliation: Agronomy Department, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000, Ljubljana, Slovenia.
authors.forename: Branka
authors.initials: B
authors.lastname: Javornik
authors.affiliation: Agronomy Department, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000, Ljubljana, Slovenia.
authors.forename: Damijana
authors.initials: D
authors.lastname: Kastelec
authors.affiliation: Slovenian Institute of Hop Research and Brewing, Cesta Žalskega tabora 2, SI-3310, Žalec, Slovenia. Electronic address: andreja.cerenak@ihps.si.
authors.forename: Andreja
authors.initials: A
authors.lastname: Čerenak
mesh_terms: D055864:Droughts; D027582:Humulus / Q000378:metabolism / Q000502:physiology*; D010788:Photosynthesis / Q000502:physiology; D010940:Plant Proteins / Q000378:metabolism; D054046:Plant Stomata / Q000502:physiology; D018526:Plant Transpiration / Q000502:physiology; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012987:Soil / Q000737:chemistry; D013312:Stress, Physiological; D014867:Water / Q000737:chemistry
chemical_list: D010940:Plant Proteins; D020543:Proteome; D012987:Soil; D014867:Water
keywords: 2D-DIGE; Drought stress; Hop (Humulus lupulus); Physiology; Proteomics