title: Generation of Lymphocytic Choriomeningitis Virus Based Vaccine Vectors.
abstract: Vaccination with a recombinant LCMV based vector expressing tumor-associated or viral antigens is a safe and versatile method to induce an immune response against tumors or viral infections. Here, we describe the generation of recombinant LCMV vectors in which the gene encoding the viral LCMV-GP was substituted with a gene of interest (vaccine antigen). This renders the vaccine vector propagation-incompetent while it preserves the property of eliciting a strong cytotoxic T cell response.
authors.affiliation: Institute of Immunobiology, Kantonsspital St. Gallen, Rorschacher Str. 95, St. Gallen, 9007, Switzerland.
authors.forename: Sandra
authors.initials: S
authors.lastname: Ring
authors.affiliation: Institute of Immunobiology, Kantonsspital St. Gallen, Rorschacher Str. 95, St. Gallen, 9007, Switzerland. lukas.flatz@gmail.com.
authors.forename: Lukas
authors.initials: L
authors.lastname: Flatz
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D000071497:Immunogenicity, Vaccine; D008217:Lymphocytic choriomeningitis virus / Q000235:genetics*; D051379:Mice; D010957:Plasmids / Q000235:genetics; D013154:Spleen / Q000276:immunology; D013194:Staining and Labeling; D014162:Transfection; D014612:Vaccines / Q000235:genetics* / Q000276:immunology
chemical_list: D004274:DNA, Recombinant; D014612:Vaccines
keywords: Arenavirus; CTL response; LCMV; Reverse genetics; Viral vaccine vectors