title: Binding of Breviscapine Toward Serum Albumin Studied by Spectroscopic and Electrochemical Techniques.
abstract: Breviscapine, a cerebrovascular drugs extracted from the Chinese herb Erigeron breviscapinus, has been frequently used to clinically treat cerebrovascular diseases such as cerebral thrombosis, cerebral infarction, and cerebral circulation insufficiency. In order to understand its pharmacology or toxicity, the binding mechanism of breviscapine to a model protein, human serum albumin (HSA), was probed by fluorescence, circular dichroism, Fourier transform infrared spectroscopy (FTIR), and electrochemical impedance spectroscopy approaches. The binding affinities and number of the drug with HSA were about 1.73 × 10(4)  M(-1) and 0.99 at 293 K, respectively. The conformation of the protein was slightly altered after interacting with breviscapine. The drug-protein complex was mainly stabilized by electrostatic forces.
authors.affiliation: Department of Neurology, Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050000, People's Republic of China. hbyoueryounv@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Affiliated Hospital of Hebei University, Baoding, Hebei, 071000, People's Republic of China.
authors.forename: Yaqing
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Radiology, Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050000, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Neurology, Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050000, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D001665:Binding Sites; D002317:Cardiovascular Agents / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D055664:Electrochemical Techniques; D049919:Erigeron / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry*; D006801:Humans; D011485:Protein Binding; D011487:Protein Conformation; D012709:Serum Albumin / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D013816:Thermodynamics
chemical_list: D002317:Cardiovascular Agents; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D012709:Serum Albumin; C061097:breviscapine
keywords: Breviscapine; Electrochemical Impedance; Human Serum Albumin; Spectroscopy