title: Antitumor efficacy of lidamycin against human multiple myeloma RPMI 8226 cells and the xenograft in nonobese diabetic/severe combined immunodeficiency mice.
abstract: OBJECTIVE
  The aim of this study is to explore the antitumor efficacy of lidamycin (LDM) against human multiple myelomas (MM).
  
  
  METHODS
  Human MM RPMI 8226 cells and the xenograft model in nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice were used to examine the antitumor activity of LDM.
  
  
  RESULTS
  Notably, LDM markedly suppressed the growth of human MM RPMI 8226 xenograft in NOD/SCID mice. In vitro, there was a significant reduction in cell proliferation after treatment with LDM. The overall growth inhibition correlated with the increase of apoptotic cells. The apoptosis-related proteins including caspase-3, 7, and 9 were activated, and poly adenosine diphosphate-ribose polymerase was cleaved. Further investigation revealed that cellular Bcl-2 and survivin decreased, whereas the level of Bax increased in the LDM-treated cells.
  
  
  CONCLUSIONS
  LDM is highly effective against the growth of MM xenograft in NOD/SCID mice. The potent apoptosis.inducing effect of LDM may be mediated through caspase. and mitochondria.dependent pathway.
authors.affiliation: Department of Histology and Embryology, Basic Medical College, Hebei United University, Hebei, Beijing, China.
authors.forename: Yongzhan
authors.initials: Y
authors.lastname: Zhen
authors.forename: Boyang
authors.initials: B
authors.lastname: Shang
authors.forename: Xiujun
authors.initials: X
authors.lastname: Liu
authors.forename: Yajun
authors.initials: Y
authors.lastname: Lin
authors.forename: Yongsu
authors.initials: Y
authors.lastname: Zhen
mesh_terms: D000617:Aminoglycosides / Q000008:administration & dosage*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000096:biosynthesis / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D053281:Enediynes / Q000008:administration & dosage*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D051379:Mice; D016688:Mice, Inbred NOD; D009101:Multiple Myeloma / Q000188:drug therapy* / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
chemical_list: D000617:Aminoglycosides; D053281:Enediynes; C058024:C 1027; D020169:Caspases