title: Extraction of Oil from Flaxseed (Linum usitatissimum L.) Using Enzyme-Assisted Three-Phase Partitioning.
abstract: In this study, enzyme-assisted three-phase partitioning (EATPP) was used to extract oil from flaxseed. The whole procedure is composed of two parts: the enzymolysis procedure in which the flaxseed was hydrolyzed using an enzyme solution (the influencing parameters such as the type and concentration of enzyme, temperature, and pH were optimized) and three-phase partitioning (TPP), which was conducted by adding salt and t-butanol to the crude flaxseed slurry, resulting in the extraction of flaxseed oil into alcohol-rich upper phase. The concentration of t-butanol, concentration of salt, and the temperature were optimized to maximize the extraction yield. Under optimized conditions of a 49.29 % t-butanol concentration, 30.43 % ammonium sulfate concentration, and 35 °C extraction temperature, a maximum extraction yield of 71.68 % was obtained. This simple and effective EATPP can be used to achieve high extraction yields and oil quality, and thus, it is potential for large-scale oil production.
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, 410205, China. tanzhijiantgzy2010@aliyun.com.
authors.forename: Zhi-Jian
authors.initials: ZJ
authors.lastname: Tan
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China.
authors.forename: Zi-Zhen
authors.initials: ZZ
authors.lastname: Yang
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, 410205, China.
authors.forename: Yong-Jian
authors.initials: YJ
authors.lastname: Yi
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, 410205, China.
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Wang
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, 410205, China.
authors.forename: Wan-Lai
authors.initials: WL
authors.lastname: Zhou
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China.
authors.forename: Fen-Fang
authors.initials: FF
authors.lastname: Li
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, 410205, China. ibfcwcy@139.com.
authors.forename: Chao-Yun
authors.initials: CY
authors.lastname: Wang
mesh_terms: D000645:Ammonium Sulfate / Q000737:chemistry; D005591:Chemical Fractionation / Q000379:methods*; D019597:Flax / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D007700:Kinetics; D008043:Linseed Oil / Q000302:isolation & purification*; D010447:Peptide Hydrolases / Q000378:metabolism*; D011096:Polygalacturonase / Q000378:metabolism*; D013696:Temperature; D020002:tert-Butyl Alcohol / Q000737:chemistry
chemical_list: D008043:Linseed Oil; D011096:Polygalacturonase; D010447:Peptide Hydrolases; D020002:tert-Butyl Alcohol; D000645:Ammonium Sulfate
keywords: Enzyme kinetics; Enzyme-assisted three-phase partitioning; Flaxseed oil; GC–MS analysis