title: Bioactive Nanocomposite Poly (Ethylene Glycol) Hydrogels Crosslinked by Multifunctional Layered Double Hydroxides Nanocrosslinkers.
abstract: Poly (ethylene glycol) (PEG) based hydrogels have been widely used in many biomedical applications such as regenerative medicine due to their good biocompatibility and negligible immunogenicity. However, bioactivation of PEG hydrogels, such as conjugation of bioactive biomolecules, is usually necessary for cell-related applications. Such biofunctionalization of PEG hydrogels generally involves complicated and time-consuming bioconjugation procedures. Herein, we describe the facile preparation of bioactive nanocomposite PEG hydrogel crosslinked by the novel multifunctional nanocrosslinkers, namely polydopamine-coated layered double hydroxides (PD-LDHs). The catechol-rich PD-LDH nanosheets not only act as effective nanocrosslinkers reinforcing the mechanical strength of the hydrogel, but also afford the hydrogels with robust bioactivity and bioadhesion via the cortical-mediated couplings. The obtained nanocomposite PEG hydrogels with the multifunctional PD-LDH crosslinking domains show tunable mechanical properties, self-healing ability, and bioadhesion to biological tissues. Furthermore, these hydrogels also promote the sequestration of proteins and support the osteogenic differentiation of human mesenchymal stem cells without any further bio-functionalization. Such facile preparation of bioactive and bioadhesive PEG hydrogels have rarely been achieved and may open up a new avenue for the design of nanocomposite PEG hydrogels for biomedical applications.
authors.affiliation: Division of Biomedical Engineering, Department of Mechanical and Automation Engineering, The Chinese University of Hong Kong Shatin, New Territories, 999077, Hong Kong, P. R. China.
authors.forename: Heqin
authors.initials: H
authors.lastname: Huang
authors.affiliation: Division of Biomedical Engineering, Department of Mechanical and Automation Engineering, The Chinese University of Hong Kong Shatin, New Territories, 999077, Hong Kong, P. R. China.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Xu
authors.affiliation: Division of Biomedical Engineering, Department of Mechanical and Automation Engineering, The Chinese University of Hong Kong Shatin, New Territories, 999077, Hong Kong, P. R. China.|Shun Hing Institute of Advanced Engineering, The Chinese University of Hong Kong, 999077, Hong Kong, P. R. China.
authors.forename: Kongchang
authors.initials: K
authors.lastname: Wei
authors.affiliation: Division of Biomedical Engineering, Department of Mechanical and Automation Engineering, The Chinese University of Hong Kong Shatin, New Territories, 999077, Hong Kong, P. R. China.
authors.forename: Yang J
authors.initials: YJ
authors.lastname: Xu
authors.affiliation: Division of Biomedical Engineering, Department of Mechanical and Automation Engineering, The Chinese University of Hong Kong Shatin, New Territories, 999077, Hong Kong, P. R. China.
authors.forename: Chun Kit K
authors.initials: CK
authors.lastname: Choi
authors.affiliation: Division of Biomedical Engineering, Department of Mechanical and Automation Engineering, The Chinese University of Hong Kong Shatin, New Territories, 999077, Hong Kong, P. R. China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Division of Biomedical Engineering, Department of Mechanical and Automation Engineering, The Chinese University of Hong Kong Shatin, New Territories, 999077, Hong Kong, P. R. China.|Shun Hing Institute of Advanced Engineering, The Chinese University of Hong Kong, 999077, Hong Kong, P. R. China.|Shenzhen Research Institute, The Chinese University of Hong Kong, 999077, Hong Kong, P. R. China.|China Orthopedic Regenerative Medicine Group (CORMed), 999077, Hong Kong, P. R. China.|Centre of Novel Biomaterials The Chinese University of Hong Kong, 999077, Hong Kong, P. R. China.
authors.forename: Liming
authors.initials: L
authors.lastname: Bian
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D006801:Humans; D020100:Hydrogels / Q000737:chemistry* / Q000494:pharmacology; D006878:Hydroxides / Q000737:chemistry / Q000494:pharmacology; D007211:Indoles / Q000737:chemistry / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000187:drug effects; D053761:Nanocomposites / Q000008:administration & dosage / Q000737:chemistry*; D010012:Osteogenesis / Q000187:drug effects*; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000737:chemistry; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D006878:Hydroxides; D007211:Indoles; D011108:Polymers; C568283:polydopamine; D011092:Polyethylene Glycols
keywords: multifunctional nanocrosslinker; nanocomposite hydrogel; osteogenic differentiation; poly (ethylene glycol); polydopamine