title: ClusterViSu, a method for clustering of protein complexes by Voronoi tessellation in super-resolution microscopy.
abstract: Super-resolution microscopy (PALM, STORM etc.) provides a plethora of fluorescent signals in dense cellular environments which can be difficult to interpret. Here we describe ClusterViSu, a method for image reconstruction, visualization and quantification of labelled protein clusters, based on Voronoi tessellation of the individual fluorescence events. The general applicability of this clustering approach for the segmentation of super-resolution microscopy data, including for co-localization, is illustrated on a series of important biological objects such as chromatin complexes, RNA polymerase, nuclear pore complexes and microtubules.
authors.affiliation: Centre for Integrative Biology (CBI), Department of Integrated Structural Biology, IGBMC (Institute of Genetics and of Molecular and Cellular Biology), 1 rue Laurent Fries, Illkirch, France.|Centre National de la Recherche Scientifique (CNRS) UMR 7104, Illkirch, France.|Institut National de la Santé et de la Recherche Médicale (INSERM) U964, Illkirch, France.|Université de Strasbourg, Strasbourg, France.
authors.forename: Leonid
authors.initials: L
authors.lastname: Andronov
authors.affiliation: Centre for Integrative Biology (CBI), Department of Integrated Structural Biology, IGBMC (Institute of Genetics and of Molecular and Cellular Biology), 1 rue Laurent Fries, Illkirch, France.|Centre National de la Recherche Scientifique (CNRS) UMR 7104, Illkirch, France.|Institut National de la Santé et de la Recherche Médicale (INSERM) U964, Illkirch, France.|Université de Strasbourg, Strasbourg, France.
authors.forename: Igor
authors.initials: I
authors.lastname: Orlov
authors.affiliation: Centre for Integrative Biology (CBI), Department of Integrated Structural Biology, IGBMC (Institute of Genetics and of Molecular and Cellular Biology), 1 rue Laurent Fries, Illkirch, France.|Centre National de la Recherche Scientifique (CNRS) UMR 7104, Illkirch, France.|Institut National de la Santé et de la Recherche Médicale (INSERM) U964, Illkirch, France.|Université de Strasbourg, Strasbourg, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Lutz
authors.affiliation: Centre for Integrative Biology (CBI), Department of Integrated Structural Biology, IGBMC (Institute of Genetics and of Molecular and Cellular Biology), 1 rue Laurent Fries, Illkirch, France.|Centre National de la Recherche Scientifique (CNRS) UMR 7104, Illkirch, France.|Institut National de la Santé et de la Recherche Médicale (INSERM) U964, Illkirch, France.|Université de Strasbourg, Strasbourg, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Vonesch
authors.affiliation: Centre for Integrative Biology (CBI), Department of Integrated Structural Biology, IGBMC (Institute of Genetics and of Molecular and Cellular Biology), 1 rue Laurent Fries, Illkirch, France.|Centre National de la Recherche Scientifique (CNRS) UMR 7104, Illkirch, France.|Institut National de la Santé et de la Recherche Médicale (INSERM) U964, Illkirch, France.|Université de Strasbourg, Strasbourg, France.
authors.forename: Bruno P
authors.initials: BP
authors.lastname: Klaholz
mesh_terms: D016000:Cluster Analysis; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D046912:Multiprotein Complexes / Q000032:analysis*
chemical_list: D046912:Multiprotein Complexes