title: Diet-Induced Weight Loss Reduces DNA Damage and Cardiometabolic Risk Factors in Overweight/Obese Women with Polycystic Ovary Syndrome.
abstract: OBJECTIVE
  We aimed to investigate the impact of following a diet to induce weight loss (500 kcal deficit per day) over DNA damage and cardiometabolic risk factors in women with overweight/obesity diagnosed with polycystic ovary syndrome (PCOS).
  
  
  METHODS
  A study was conducted in Natal, RN, Brazil selecting overweight/obese (body mass index ≥25 and <39 kg/m2) women (18-35 years). The levels of DNA damage were assessed by a single cell gel electrophoresis. Repeated 24 h dietary recall questionnaires, anthropometry, biochemical profile and sex hormones were collected at baseline and after 12 weeks of intervention.
  
  
  RESULTS
  Women exhibiting a decrease in the markers of DNA damage: tail intensity (24.35 ± 5.86 - pre diet vs. 17.15 ± 5.04 - post-diet; p < 0.001) and tail moment (20.47 ± 7.85 - pre diet vs. 14.13 ± 6.29 - post-diet; p < 0.002). Reduction of calorie intake, weight loss, decreased sexual hormone and cardiometabolic markers such as insulin, homeostasis model assessment of insulin resistance and low-density lipoprotein cholesterol were verified In the multivariate regression analysis, quantitative insulin sensitivity check index and progesterone were responsible for the variation markers in DNA damage before the diet, losing its influence upon diet.
  
  
  CONCLUSIONS
  DNA damage and the impact of cardiometabolic risk factors decreased after the intervention in women with PCOS, indicating the relevance of a nutritional approach in this group of patients.
authors.affiliation: Department of Clinical and Toxicological, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
authors.forename: Nayara Pereira
authors.initials: NP
authors.lastname: Soares
authors.forename: Ana Celly Souza dos
authors.initials: AC
authors.lastname: Santos
authors.forename: Eduardo Caldas
authors.initials: EC
authors.lastname: Costa
authors.forename: George Dantas
authors.initials: GD
authors.lastname: Azevedo
authors.forename: Débora Cristina
authors.initials: DC
authors.lastname: Damasceno
authors.forename: Ana Paula Trussardi
authors.initials: AP
authors.lastname: Fayh
authors.forename: Telma Maria Araújo Moura
authors.initials: TM
authors.lastname: Lemos
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000097:blood; D015992:Body Mass Index; D001938:Brazil; D002318:Cardiovascular Diseases / Q000453:epidemiology / Q000209:etiology / Q000517:prevention & control*; D020552:Comet Assay; D004249:DNA Damage; D004038:Diet, Reducing; D005260:Female; D006801:Humans; D006949:Hyperlipidemias / Q000453:epidemiology / Q000209:etiology / Q000517:prevention & control; D007333:Insulin Resistance; D024821:Metabolic Syndrome / Q000453:epidemiology / Q000209:etiology / Q000517:prevention & control*; D009765:Obesity / Q000150:complications / Q000178:diet therapy* / Q000378:metabolism / Q000503:physiopathology; D050177:Overweight / Q000150:complications / Q000178:diet therapy* / Q000378:metabolism / Q000503:physiopathology; D010352:Patient Dropouts; D011085:Polycystic Ovary Syndrome / Q000150:complications / Q000178:diet therapy* / Q000378:metabolism / Q000503:physiopathology; D012307:Risk Factors; D015431:Weight Loss; D055815:Young Adult
chemical_list: D015415:Biomarkers