title: Allelochemicals of the phenoxazinone class act at physiologically relevant concentrations.
abstract: Plants compete with their neighbors via the release of chemical compounds into the rhizosphere. These phytotoxins originate from a series of secondary metabolites and can be processed further by soil-living microorganisms before exerting their activity on the target plant. To determine the molecular mode of action and the physiological relevance of potential phytotoxins, it is important to simulate true-to-life conditions in laboratory experiments, for example by applying physiologically relevant concentrations. Here, we report on an improved experimental setting to study the function of allelochemicals of the benzoxazolinone class. By adjusting the solvent and the application of the chemicals, we reduced by more than 2fold the concentration that is necessary to induce growth defects in the model plant Arabidopsis thaliana.
authors.affiliation: a Department of Internal Medicine I , Medical University Clinic, University of Tübingen , Tübingen , Germany.
authors.forename: Sascha
authors.initials: S
authors.lastname: Venturelli
authors.affiliation: a Department of Internal Medicine I , Medical University Clinic, University of Tübingen , Tübingen , Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Petersen
authors.affiliation: b Department of Molecular Biology , Max Planck Institute for Developmental Biology , Tübingen , Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Langenecker
authors.affiliation: b Department of Molecular Biology , Max Planck Institute for Developmental Biology , Tübingen , Germany.
authors.forename: Detlef
authors.initials: D
authors.lastname: Weigel
authors.affiliation: a Department of Internal Medicine I , Medical University Clinic, University of Tübingen , Tübingen , Germany.
authors.forename: Ulrich M
authors.initials: UM
authors.lastname: Lauer
authors.affiliation: b Department of Molecular Biology , Max Planck Institute for Developmental Biology , Tübingen , Germany.
authors.forename: Claude
authors.initials: C
authors.lastname: Becker
mesh_terms: D000107:Acetylation / Q000187:drug effects; D017360:Arabidopsis / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D003470:Culture Media / Q000494:pharmacology; D006657:Histones / Q000378:metabolism; D008954:Models, Biological; D010078:Oxazines / Q000494:pharmacology*; D010675:Pheromones / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development
chemical_list: D003470:Culture Media; D006657:Histones; D010078:Oxazines; D010675:Pheromones; C020456:phenoxazinone
keywords: Allelochemical; Arabidopsis thaliana; allelopathy; aminophenoxazinone; plant-plant interference