title: Understanding metabolism of arginine in biological systems via MALDI imaging.
abstract: Arginine is an important amino acid but has been barely studied in plants. The little research that has been done indicates that the pathways of synthesis are similar to those found in animals and procaryotes. However little is known about the cellular and tissue localization of the amino acid in plants. The research reported in this paper was designed to examine whether MALDI-MSI was sufficiently sensitive to examine the distribution of this amino acid in plant material, and whether the synthetic pathways were co-located. In wheat and orchid roots, the amount of arginine in tissues varies greatly and the pathways for its synthesis were not always detected with the amino acid.
authors.affiliation: Dept. Animal and Plant Sciences, Sheffield University, Western Bank, Sheffield, UK.
authors.forename: Heather J
authors.initials: HJ
authors.lastname: Walker
authors.affiliation: Dept. Animal and Plant Sciences, Sheffield University, Western Bank, Sheffield, UK.
authors.forename: Chloe
authors.initials: C
authors.lastname: Steels
authors.affiliation: Dept. Animal and Plant Sciences, Sheffield University, Western Bank, Sheffield, UK.
authors.forename: Lilias
authors.initials: L
authors.lastname: Bendell
authors.affiliation: Biomedical Research Centre, Sheffield Hallam University, Howard Street, Sheffield, UK.
authors.forename: Malcolm R
authors.initials: MR
authors.lastname: Clench
authors.affiliation: Dept. Animal and Plant Sciences, Sheffield University, Western Bank, Sheffield, UK.
authors.forename: David J
authors.initials: DJ
authors.lastname: Read
authors.affiliation: Dept. Animal and Plant Sciences, Sheffield University, Western Bank, Sheffield, UK.
authors.forename: Duncan D
authors.initials: DD
authors.lastname: Cameron
authors.affiliation: Dept. Animal and Plant Sciences, Sheffield University, Western Bank, Sheffield, UK.
authors.forename: Michael M
authors.initials: MM
authors.lastname: Burrell
mesh_terms: D001120:Arginine / Q000096:biosynthesis* / Q000378:metabolism; D057054:Molecular Imaging / Q000379:methods*; D029595:Orchidaceae / Q000737:chemistry / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014908:Triticum / Q000737:chemistry / Q000378:metabolism
chemical_list: D001120:Arginine
keywords: Amino acid; Arginine; Orchid; Ornithine; Plant; Plant development; Plant metabolism; Technology; Wheat