title: [¹H-NMR based metabonomic approach to evaluate anti-coagulant effect of Danggui Sini decoction].
abstract: To study the anti-coagulant effect and influence of danggui Sini decoction (DSD) on rat's plasma endogenous metabolites by animal experiment and ¹H-NMR based metabolomics method. After intragastric administration of Danggui Sini Decoction for 7 days, Plasma thrombin time (TT) was measured. Rat plasma metabolic fingerprint in two groups was analyzed using ¹H-NMR, based on which the principal component analysis( PCA) and orthogonal partial least-squares discriminant analysis(OPLS-DA) models for metabonomic analysis. Potential biomarkers were screened by using variable importance in the projection (VIP) and T test. DSD could prolong TT of the rat significantly (P < 0.05). Compared with control group, six kinds of endogenous metabolites in DSD group change significantly (P < 0.05), among which isobutyrate, carnitine and phenylalanine content had an upward trend (P < 0.01) and lysine, Histidine and cholesterol content had a downward trend (P < 0.05). It is likely that carnitine, phenylalanine, Histidine and cholesterol are the potential metabolic markers in the anti-coagulant process and DSD affects the platelet aggregation and the expression of tissue factor and fiber protease by regulating the energy, amino acid and lipid metabolism.
authors.forename: Hua
authors.initials: H
authors.lastname: Zheng
authors.forename: Xia
authors.initials: X
authors.lastname: Qin
authors.forename: Hui
authors.initials: H
authors.lastname: Song
authors.forename: Chao-lin
authors.initials: CL
authors.lastname: Tang
authors.forename: Jun-xiang
authors.initials: JX
authors.lastname: Ruan
authors.forename: Hong-ye
authors.initials: HY
authors.lastname: Zhang
authors.forename: Shi-yin
authors.initials: SY
authors.lastname: Lu
authors.forename: Yong-hong
authors.initials: YH
authors.lastname: Liang
authors.forename: Zhi-heng
authors.initials: ZH
authors.lastname: Su
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000737:chemistry*; D001777:Blood Coagulation / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005260:Female; D008297:Male; D055432:Metabolomics / Q000379:methods*; D066244:Proton Magnetic Resonance Spectroscopy / Q000379:methods*; D051381:Rats
chemical_list: D000925:Anticoagulants; D004365:Drugs, Chinese Herbal