title: Comparison of Matrix-Based and Filter Paper-Based Systems for Transport of Plasma for HIV-1 RNA Quantification and Amplicon Preparation for Genotyping.
abstract: Two ambient-temperature, dry plasma transport systems, ViveST tubes and RNASound RNA sampling cards, and two extraction methods were compared to frozen plasma for HIV-1 RNA recovery. Significant RNA loss occurred: ViveST+MiniMag > ViveST+QIAamp > RNASound+QIAamp. RNA loss and low specimen volumes may affect the sensitivity of genotyping specimens with HIV-1 RNA of <4.70 log10 copies/ml.
authors.affiliation: Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA.
authors.forename: Molly
authors.initials: M
authors.lastname: Levine
authors.affiliation: Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Beck
authors.affiliation: Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA.
authors.forename: Sheila
authors.initials: S
authors.lastname: Styrchak
authors.affiliation: University of Washington, Seattle, Washington, USA.
authors.forename: Gregory
authors.initials: G
authors.lastname: Pepper
authors.affiliation: Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA lfrenkel@uw.edu.|University of Washington, Seattle, Washington, USA.
authors.forename: Lisa
authors.initials: L
authors.lastname: Frenkel
mesh_terms: D060005:Genotyping Techniques / Q000379:methods*; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D010949:Plasma / Q000821:virology; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*; D019562:Viral Load / Q000379:methods*
chemical_list: D012367:RNA, Viral