title: Tetanus neurotoxin HCC protein commits T cells to IFN-γ producing cells.
abstract: A protective response against tetanus toxin and toxoid demands efficient specific T cell and B cell responses. Tetanus neurotoxin (TeNT), a 150 kDa polypeptide, is the main cause of tetanus disease. TeNT consists of two structurally distinct chains, a 50 kDa N-terminal light (L) and a 100 kDa C-terminal heavy (H) chain. C-terminal heavy (H) chain (fragment C) has two sub-domains named as proximal HCN and carboxy sub-domain or HCC. Beside neural binding property, HCC has been recently found as an immunodominant module of TeNT. In the present study, we investigated the effects of recombinant HCC (rHCC) on the expression of lineage specific transcription factors and secretion of a panel of functional cytokines including IFN-γ, IL-4, and IL-17 from purified human T cells. Our results revealed that T-bet transcript level, as TH1 specific transcription factor, was significantly increased in the cells treated with 10 and 20 µg/ml of rHCC following 48 h treatment(p<0.05). Treated purified human T cells with rHCC showed significant increase in IFN-γ mRNA level and cytokine secretion, but not IL-4 and IL-17, following 48 h treatment. In conclusion, our results showed that treatment of T cells with r HCC resulted in development of Th1 lineage phenotype, which might lead to a specific and protective antibody mediated response against tetanus toxin.
authors.affiliation: Tabriz University of Medical Sciences Drug Applied Research Center Tabriz Iran.
authors.forename: S
authors.initials: S
authors.lastname: Torabi Goudarzi
authors.affiliation: Tabriz University of Medical Sciences Drug Applied Research Center Tabriz Iran.
authors.forename: M
authors.initials: M
authors.lastname: Hajivalili
authors.affiliation: Tabriz University of Medical Sciences Immunology Research Center Tabriz Iran.
authors.forename: M
authors.initials: M
authors.lastname: Hosseini
authors.affiliation: Tabriz University of Medical Sciences Immunology Research Center Tabriz Iran.
authors.forename: M
authors.initials: M
authors.lastname: Ghafari Khamene
authors.affiliation: Golestan University of Medical Sciences Infectious Diseases Research Center and Laboratory Science Research Center Gorgan Iran.
authors.forename: Y
authors.initials: Y
authors.lastname: Yazdani
authors.affiliation: Tabriz University of Medical Sciences Immunology Research Center Tabriz Iran.
authors.forename: S
authors.initials: S
authors.lastname: Sadreddini
authors.affiliation: Alborz University of Medical Sciences Department of Medical Parasitology and Mycology, School of Medicine Karaj Iran.
authors.forename: A
authors.initials: A
authors.lastname: Miahipour
authors.affiliation: Avicenna research institute, ACESR Monoclonal antibody research center Tehran Iran.
authors.forename: V
authors.initials: V
authors.lastname: Younesi
authors.affiliation: Tabriz University of Medical Sciences Department of Immunology, Faculty of Medicine Tabriz Iran Yousefime@tbzmed.ac.ir.
authors.forename: M
authors.initials: M
authors.lastname: Yousefi
mesh_terms: D000328:Adult; D003017:Clostridium tetani / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology*; D020381:Interleukin-17 / Q000276:immunology*; D015847:Interleukin-4 / Q000276:immunology; D008666:Metalloendopeptidases / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins / Q000276:immunology; D020825:T-Box Domain Proteins / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D013742:Tetanus / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D013744:Tetanus Toxin / Q000276:immunology*; D015533:Transcriptional Activation; D000098815:T-bet Transcription Factor
chemical_list: C508598:IL4 protein, human; D020381:Interleukin-17; D012333:RNA, Messenger; D011994:Recombinant Proteins; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D013744:Tetanus Toxin; C035979:tetanospasmin; D015847:Interleukin-4; D007371:Interferon-gamma; D008666:Metalloendopeptidases