title: Antimicrobial Activity of Nisin and Natamycin Incorporated Sodium Caseinate Extrusion-Blown Films: A Comparative Study with Heat-Pressed/Solution Cast Films.
abstract: Antimicrobial edible films based on sodium caseinate, glycerol, and 2 food preservatives (nisin or natamycin) were prepared by classical thermomechanical processes. Food preservatives were compounded (at 65 °C for 2.5 min) with sodium caseinate in a twin-screw extruder. Anti-Listeria activity assays revealed a partial inactivation of nisin following compounding. Thermoplastic pellets containing food preservatives were then used to manufacture films either by blown-film extrusion process or by heat-press. After 24 h of incubation on agar plates, the diameters of K. rhizophila growth inhibition zones around nisin-incorporated films prepared by solution casting (control), extrusion blowing or heat pressing at 80 °C for 7 min of nisin-containing pellets were 15.5 ± 0.9, 9.8 ± 0.2, and 8.6 ± 1.0 mm, respectively. Since heat-pressing for 7 min at 80 °C of nisin-incorporated pellets did not further inactivate nisin, this indicates that nisin inactivation during extrusion-blowing was limited. Moreover, the lower diameter of the K. rhizophila growth inhibition zone around films prepared with nisin-containing pellets compared to that observed around films directly prepared by solution casting confirms that nisin inactivation mainly occurred during the compounding step. Natamycin-containing thermoplastic films inhibited Aspergillus niger growth; however, by contrast with nisin-containing films, heat-pressed films had higher inhibition zone diameters than blown films, therefore suggesting a partial inactivation of natamycin during extrusion-blowing.
authors.affiliation: Univ. de Lyon, Université Jean Monnet, CNRS UMR (Joined Research Unit) 5223, Ingénierie des Matériaux Polymères, 23 rue Paul Michelon. F-42023, Saint-Etienne, France.|Univ. de Lyon, Univ. Claude Bernard Lyon 1 , ISARA Lyon, Laboratoire de Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires (BioDyMIA, EA n°3733), IUT Lyon 1 site de Bourg en Bresse, technopole Alimentec, rue Henri de Boissieu, F-01000, Bourg en Bresse, France.|Univ. de Saint-Etienne, F-42023, Saint-Etienne, France.|Univ. de Lyon, Univ. Claude Bernard Lyon 1 - ISARA Lyon, Laboratoire de Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires (BioDyMIA, EA n°3733), IUT Lyon 1 site de Bourg en Bresse, technopole Alimentec, rue Henri de Boissieu, F-01000 Bourg en Bresse, France.
authors.forename: Basak Yilin
authors.initials: BY
authors.lastname: Colak
authors.affiliation: Univ. de Lyon, Univ. Claude Bernard Lyon 1 , ISARA Lyon, Laboratoire de Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires (BioDyMIA, EA n°3733), IUT Lyon 1 site de Bourg en Bresse, technopole Alimentec, rue Henri de Boissieu, F-01000, Bourg en Bresse, France.|Univ. de Lyon, Univ. Claude Bernard Lyon 1 - ISARA Lyon, Laboratoire de Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires (BioDyMIA, EA n°3733), IUT Lyon 1 site de Bourg en Bresse, technopole Alimentec, rue Henri de Boissieu, F-01000 Bourg en Bresse, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Peynichou
authors.affiliation: Univ. de Lyon, Univ. Claude Bernard Lyon 1 , ISARA Lyon, Laboratoire de Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires (BioDyMIA, EA n°3733), IUT Lyon 1 site de Bourg en Bresse, technopole Alimentec, rue Henri de Boissieu, F-01000, Bourg en Bresse, France.|Univ. de Lyon, Univ. Claude Bernard Lyon 1 - ISARA Lyon, Laboratoire de Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires (BioDyMIA, EA n°3733), IUT Lyon 1 site de Bourg en Bresse, technopole Alimentec, rue Henri de Boissieu, F-01000 Bourg en Bresse, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Galland
authors.affiliation: Univ. de Lyon, Univ. Claude Bernard Lyon 1 , ISARA Lyon, Laboratoire de Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires (BioDyMIA, EA n°3733), IUT Lyon 1 site de Bourg en Bresse, technopole Alimentec, rue Henri de Boissieu, F-01000, Bourg en Bresse, France.|Univ. de Lyon, Univ. Claude Bernard Lyon 1 - ISARA Lyon, Laboratoire de Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires (BioDyMIA, EA n°3733), IUT Lyon 1 site de Bourg en Bresse, technopole Alimentec, rue Henri de Boissieu, F-01000 Bourg en Bresse, France.
authors.forename: Nadia
authors.initials: N
authors.lastname: Oulahal
authors.affiliation: Univ. de Lyon, Université Jean Monnet, CNRS UMR (Joined Research Unit) 5223, Ingénierie des Matériaux Polymères, 23 rue Paul Michelon. F-42023, Saint-Etienne, France.|Univ. de Saint-Etienne, F-42023, Saint-Etienne, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Prochazka
authors.affiliation: Univ. de Lyon, Univ. Claude Bernard Lyon 1 , ISARA Lyon, Laboratoire de Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires (BioDyMIA, EA n°3733), IUT Lyon 1 site de Bourg en Bresse, technopole Alimentec, rue Henri de Boissieu, F-01000, Bourg en Bresse, France.|Univ. de Lyon, Univ. Claude Bernard Lyon 1 - ISARA Lyon, Laboratoire de Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires (BioDyMIA, EA n°3733), IUT Lyon 1 site de Bourg en Bresse, technopole Alimentec, rue Henri de Boissieu, F-01000 Bourg en Bresse, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Degraeve
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D001234:Aspergillus niger / Q000187:drug effects / Q000254:growth & development; D063566:Biodegradable Plastics / Q000737:chemistry; D002364:Caseins; D005516:Food Microbiology; D018857:Food Packaging / Q000379:methods; D005520:Food Preservatives / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D008089:Listeria monocytogenes / Q000187:drug effects / Q000254:growth & development; D010866:Natamycin / Q000494:pharmacology*; D009561:Nisin / Q000494:pharmacology*; D011312:Pressure; D012996:Solutions
chemical_list: D000890:Anti-Infective Agents; D063566:Biodegradable Plastics; D002364:Caseins; D005520:Food Preservatives; D012996:Solutions; D009561:Nisin; D010866:Natamycin
keywords: caseinate; edible films; extrusion; natamycin; nisin