title: A Drosophila heat shock response represents an exception rather than a rule amongst Diptera species.
abstract: Heat shock protein 70 (Hsp70) is the major player that underlies adaptive response to hyperthermia in all organisms studied to date. We investigated patterns of Hsp70 expression in larvae of dipteran species collected from natural populations of species belonging to four families from different evolutionary lineages of the order Diptera: Stratiomyidae, Tabanidae, Chironomidae and Ceratopogonidae. All investigated species showed a Hsp70 expression pattern that was different from the pattern in Drosophila. In contrast to Drosophila, all of the species in the families studied were characterized by high constitutive levels of Hsp70, which was more stable than that in Drosophila. When Stratiomyidae Hsp70 proteins were expressed in Drosophila cells, they became as short-lived as the endogenous Hsp70. Interestingly, three species of Ceratopogonidae and a cold-water species of Chironomidae exhibited high constitutive levels of Hsp70 mRNA and high basal levels of Hsp70. Furthermore, two species of Tabanidae were characterized by significant constitutive levels of Hsp70 and highly stable Hsp70 mRNA. In most cases, heat-resistant species were characterized by a higher basal level of Hsp70 than more thermosensitive species. These data suggest that different trends were realized during the evolution of the molecular mechanisms underlying the regulation of the responses of Hsp70 genes to temperature fluctuations in the studied families.
authors.affiliation: Engelgardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: O G
authors.initials: OG
authors.lastname: Zatsepina
authors.affiliation: Zoological Institute, Russian Academy of Sciences, St. Petersburg, Russia.|Tyumen State University, Tyumen, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Przhiboro
authors.affiliation: Engelgardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: I A
authors.initials: IA
authors.lastname: Yushenova
authors.affiliation: Engelgardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: V
authors.initials: V
authors.lastname: Shilova
authors.affiliation: Engelgardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: E S
authors.initials: ES
authors.lastname: Zelentsova
authors.affiliation: Engelgardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: N G
authors.initials: NG
authors.lastname: Shostak
authors.affiliation: Engelgardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: M B
authors.initials: MB
authors.lastname: Evgen'ev
authors.affiliation: Engelgardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: D G
authors.initials: DG
authors.lastname: Garbuz
mesh_terms: D000818:Animals; D005075:Biological Evolution; D004175:Diptera / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D004331:Drosophila melanogaster / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D018869:Heat-Shock Response; D007814:Larva / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity
chemical_list: D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger
keywords: Ceratopogonidae; Chironomidae; Diptera; Drosophila; Stratiomyidae; Tabanidae; heat shock protein 70; heat shock response; larvae; thermoresistance