title: An Xpert screen to identify carbapenemases.
abstract: To prevent the spread of carbapenemases-producing Enterobacteriaceae (CPE) active surveillance, contact isolation and cohorting infected patients should be practiced. Rectal swabs for the Xpert MDRO-assay of 32 patients were included. 71.85% were positive for targets incorporated into the MDRO-assay; whereas 28% were phenotypically not CRE and Xpert negative (9.37% had different mechanism [bla OXA]). The assay identified 59.3%, 9.37% and 3.1% as bla NDM, bla NDM+VIM and bla VIM, respectively. The assay is a screening test that identifies CPE harbouring organism within an hour and can be installed at tertiary-care facilities to screen colonized patients.
authors.forename: Mubin
authors.initials: M
authors.lastname: Kazi
authors.forename: Chaitali
authors.initials: C
authors.lastname: Nikam
authors.forename: Anjali
authors.initials: A
authors.lastname: Shetty
authors.affiliation: Department of Microbiology, P. D. Hinduja National Hospital and Medical Research Centre, Mahim, Mumbai, Maharashtra, India.
authors.forename: Camilla
authors.initials: C
authors.lastname: Rodrigues
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D001431:Bacteriological Techniques / Q000379:methods*; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D006801:Humans; D008403:Mass Screening / Q000379:methods; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012007:Rectum / Q000382:microbiology; D001618:beta-Lactamases / Q000032:analysis* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase