title: The Effect of Endogenous Adenosine on Neuronal Activity in Rats: An FDG PET Study.
abstract: 2-(18) F-fluorodeoxy-D-glucose (FDG) is a glucose analog that is taken up by cells and phosphorylated. The amount of FDG accumulated by cells is a measure of the rate of glycolysis, which reflects cellular activity. As the levels and actions of the neuromodulator adenosine are dynamically regulated by neuronal activity, this study was designed to test whether endogenous adenosine affects tissue accumulation of FDG as assessed by positron emission tomography (PET) or by postmortem analysis of tissue radioactivity. Rats were given an intraperitoneal injection of the adenosine A1 receptor antagonist 8-cyclopentyl-1,3-dipropyl-xanthine (DPCPX, 3 mg/kg), the adenosine kinase inhibitor ABT-702 (3 mg/kg), or vehicle 10 minutes prior to an intravenous injection of FDG (15.4 ± 0.7 MBq per rat). Rats were then subjected to a 15 minute static PET scan. Reconstructed images were normalized to FDG PET template for rats and standard uptake values (SUVs) were calculated. To examine the regional effect of active treatment compared to vehicle, statistical parametric mapping analysis was performed. Whole-brain FDG uptake was not affected by drug treatment. Significant regional hypometabolism was detected, particularly in cerebellum, of DPCPX- and ABT-702 treated rats, relative to vehicle-treated rats. Thus, endogenous adenosine can affect FDG accumulation although this effect is modest in quiescent rats.
authors.affiliation: Departments of Pharmacology and Therapeutics, University of Manitoba, Winnipeg, MB, Canada.|Neuroscience Research Program, Health Sciences Centre, Winnipeg, MB, Canada.
authors.forename: Fiona E
authors.initials: FE
authors.lastname: Parkinson
authors.affiliation: Departments of Pharmacology and Therapeutics, University of Manitoba, Winnipeg, MB, Canada.|Neuroscience Research Program, Health Sciences Centre, Winnipeg, MB, Canada.
authors.forename: Soumen
authors.initials: S
authors.lastname: Paul
authors.affiliation: Departments of Human Anatomy and Cell Science, University of Manitoba, Winnipeg, MB, Canada.|Neuroscience Research Program, Health Sciences Centre, Winnipeg, MB, Canada.
authors.forename: Dali
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Departments of Radiology, University of Manitoba, Winnipeg, MB, Canada.
authors.forename: Shadreck
authors.initials: S
authors.lastname: Mzengeza
authors.affiliation: Departments of Human Anatomy and Cell Science, University of Manitoba, Winnipeg, MB, Canada.|Neuroscience Research Program, Health Sciences Centre, Winnipeg, MB, Canada.
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Ko
mesh_terms: D000241:Adenosine / Q000502:physiology*; D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging* / Q000502:physiology*; D001931:Brain Mapping; D019788:Fluorodeoxyglucose F18; D005947:Glucose; D006019:Glycolysis / Q000502:physiology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D009025:Morpholines / Q000494:pharmacology; D049268:Positron-Emission Tomography / Q000379:methods; D011743:Pyrimidines / Q000494:pharmacology; D051381:Rats; D043682:Receptor, Adenosine A1 / Q000187:drug effects; D014970:Xanthines / Q000494:pharmacology
chemical_list: D009025:Morpholines; D011743:Pyrimidines; D043682:Receptor, Adenosine A1; D014970:Xanthines; D019788:Fluorodeoxyglucose F18; C416933:ABT 702; C051360:1,3-dipropyl-8-cyclopentylxanthine; D005947:Glucose; D000241:Adenosine
keywords: Adenosine kinase; adenosine A1 receptor; in vivo imaging