title: Preparation and Characterization of PLGA Encapsulated Protective Antigen Domain 4 Nanoformulation.
abstract: Poly (lactic-co-glycolic acid) (PLGA) based particulate systems have been widely explored for the development of subunit based vaccines owing to its biodegradability, biocompatibility, controlled release of entrapped antigen, targeted delivery potential, and nontoxic degradation product. Here, we describe the preparation of PLGA encapsulated recombinant protective antigen domain 4 (PAD4) nanoformulation (PAD4-NP) and its characterization for antigen content, morphology, and size. We also discuss the method of immunization in mice models to evaluate such PLGA based nanoformulation vaccines.
authors.affiliation: Laboratory of Molecular Biology and Genetic Engineering, School of Biotechnology, Jawaharlal Nehru University, New Delhi, 110067, India.|Department of Biosciences, Manipal University Jaipur, Jaipur-Ajmer Express Highway, Jaipur, Rajasthan, 303007, India.
authors.forename: Manish
authors.initials: M
authors.lastname: Manish
authors.affiliation: Laboratory of Molecular Biology and Genetic Engineering, School of Biotechnology, Jawaharlal Nehru University, New Delhi, 110067, India.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Bhatnagar
authors.affiliation: Department of Microbial Biotechnology, Panjab University, Chandigarh, 160014, India. samer@pu.ac.in.
authors.forename: Samer
authors.initials: S
authors.lastname: Singh
mesh_terms: D000818:Animals; D002214:Capsules; D004339:Drug Compounding / Q000379:methods*; D005260:Female; D006867:Hydrolases / Q000737:chemistry* / Q000276:immunology; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D019344:Lactic Acid / Q000737:chemistry*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D000080002:Protein-Arginine Deiminase Type 4; D000076342:Protein-Arginine Deiminases
chemical_list: D002214:Capsules; D007074:Immunoglobulin G; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D006867:Hydrolases; D000080002:Protein-Arginine Deiminase Type 4; D000076342:Protein-Arginine Deiminases; C552151:peptidylarginine deiminase 4, mouse
keywords: PLGA based nanoparticles; Recombinant PAD4; Single dose; Vaccine; W/O/W solvent evaporation method