title: Resveratrol-loaded nanocapsules inhibit murine melanoma tumor growth.
abstract: In this study, resveratrol-loaded nanocapsules were developed and its antitumor activity tested on a melanoma mice model. These nanocapsules were spherically-shaped and presented suitable size, negative charge and high encapsulation efficiency for their use as a modified-release system of resveratrol. Nanoencapsulation leads to the drug amorphization. Resveratrol-loaded nanoparticles reduced cell viability of murine melanoma cells. There was a decrease in tumor volume, an increase in the necrotic area and inflammatory infiltrate of melanoma when resveratrol-loaded nanocapsules were compared to free resveratrol in treated mice. Nanoencapsulation of resveratrol also prevented metastasis and pulmonary hemorrhage. This modified-release technology containing resveratrol can be used as a feasible approach in order to inhibit murine melanoma tumor growth.
authors.affiliation: Postgraduate Program in Pharmaceutical Sciences, Ponta Grossa State University/Midwestern State University, Brazil.
authors.forename: Bruna
authors.initials: B
authors.lastname: Carletto
authors.affiliation: Postgraduate Program in Pharmaceutical Sciences, Ponta Grossa State University/Midwestern State University, Brazil.
authors.forename: Juliana
authors.initials: J
authors.lastname: Berton
authors.affiliation: Postgraduate Program in Pharmaceutical Sciences, Ponta Grossa State University/Midwestern State University, Brazil.
authors.forename: Tamara Nascimento
authors.initials: TN
authors.lastname: Ferreira
authors.affiliation: Postgraduate Program in Pharmaceutical Sciences, Ponta Grossa State University/Midwestern State University, Brazil.
authors.forename: Luciana Facco
authors.initials: LF
authors.lastname: Dalmolin
authors.affiliation: Department of Structural, Molecular and Genetic Biology, Ponta Grossa State University, Brazil.
authors.forename: Katia Sabrina
authors.initials: KS
authors.lastname: Paludo
authors.affiliation: Department of Pharmacy, MidwesternState University, Brazil.
authors.forename: Rubiana Mara
authors.initials: RM
authors.lastname: Mainardes
authors.affiliation: Department of Pharmaceutical Sciences, Ponta Grossa State University, Brazil.
authors.forename: Paulo Vitor
authors.initials: PV
authors.lastname: Farago
authors.affiliation: Departmentof General Biology, Ponta Grossa State University, Brazil. Electronic address: gmfavero@uepg.br.
authors.forename: Giovani Marino
authors.initials: GM
authors.lastname: Favero
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D008546:Melanoma, Experimental / Q000473:pathology*; D008810:Mice, Inbred C57BL; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D047368:Tumor Burden / Q000187:drug effects; D014961:X-Ray Diffraction
chemical_list: D053769:Nanocapsules; D013267:Stilbenes; D000077185:Resveratrol
keywords: B16F10 Cell line; Nanoparticles; PCL; Phytoalexin; Polymeric drug carrier