title: Production and optimization of curdlan produced by Pseudomonas sp. QL212.
abstract: Curdlan is a polysaccharide that consists of β-1,3-linked glucose residues. A polysaccharide-producing bacterium isolated from soil samples was identified as Pseudomonas sp. QL212. The polysaccharide was purified to homogeneity via sequential ethanol precipitation, deproteinization, CM ion-exchange, and gel chromatography sequentially. Analysis of the purified polysaccharide revealed that it consisted of many glucosyl residues, and its molecular weight was only 6.18×10(5)Da. This low molecular weight endowed it with excellent solubility. Infrared and nuclear magnetic resonance spectral analysis confirmed that the polysaccharide was curdlan. Single-factor and Response surface methodology experiments were used to optimize the culture medium and conditions. The optimal culture conditions consisted of seed culture age of 12h, and an incubation temperature of 30°C, with 10% inoculum and a total fluid volume of 75mL in a 250-mL Erlenmeyer flask. The maximum curdlan yield of about 5.92gL(-1) was achieved with an optimal medium consisting of 30.11gL(-1) of sucrose, 5.94gL(-1) of yeast extract, and an initial pH of 8.03. To our best knowledge, this is the highest reported yield of curdlan produced by Pseudomonas sp., and the curdlan production medium components were much simpler than those in previous reports.
authors.affiliation: School of Biological Science and Technology, University of Jinan, Jinan, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.affiliation: New Materials Research Institute of Shandong Academy of Sciences, Jinan, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: School of Biological Science and Technology, University of Jinan, Jinan, China; Xinjiang Prodution & Construction Group Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Alar, China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Biological Science and Technology, University of Jinan, Jinan, China; Xinjiang Prodution & Construction Group Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Alar, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Bao
authors.affiliation: School of Biological Science and Technology, University of Jinan, Jinan, China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Fan
authors.affiliation: School of Biological Science and Technology, University of Jinan, Jinan, China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Qu
authors.affiliation: School of Biological Science and Technology, University of Jinan, Jinan, China.
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Shandong Academy of Medical Sciences, Jinan, China.
authors.forename: Zhiheng
authors.initials: Z
authors.lastname: Hu
authors.affiliation: School of Biological Science and Technology, University of Jinan, Jinan, China. Electronic address: lq_ujn@126.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
mesh_terms: D000704:Analysis of Variance; D002244:Carbon / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009584:Nitrogen / Q000494:pharmacology; D010802:Phylogeny; D066244:Proton Magnetic Resonance Spectroscopy; D011549:Pseudomonas / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D047071:beta-Glucans / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D047071:beta-Glucans; C038459:curdlan; D002244:Carbon; D009584:Nitrogen
keywords: Curdlan; Optimization; Pseudomonas sp