title: Short communication: The role of autoinducer 2 (AI-2) on antibiotic resistance regulation in an Escherichia coli strain isolated from a dairy cow with mastitis.
abstract: Extended spectrum β-lactamase (ESBL)-positive Escherichia coli is a major etiological organism responsible for bovine mastitis. The autoinducer 2 (AI-2) quorum sensing system is widely present in many species of gram-negative and gram-positive bacteria and has been proposed to be involved in interspecies communication. In E. coli model strains, the functional mechanisms of AI-2 have been well studied; however, in clinical antibiotic-resistant E. coli strains, whether AI-2 affects the expression of antibiotic resistance genes has not been reported. In this study, we report that exogenous AI-2 increased the antibiotic resistance of a clinical E. coli strain isolated from a dairy cow with mastitis by upregulating the expression of TEM-type enzyme in an LsrR (LuxS regulated repressor)-dependent manner.
authors.affiliation: School of Life Sciences, Anhui Agricultural University, Hefei, Anhui 230036, China. Electronic address: xuet@ahau.edu.cn.
authors.forename: Ting
authors.initials: T
authors.lastname: Xue
authors.affiliation: School of Life Sciences, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors.forename: Lumin
authors.initials: L
authors.lastname: Yu
authors.affiliation: School of Life Sciences, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Shang
authors.affiliation: School of Life Sciences, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors.forename: Wenchang
authors.initials: W
authors.lastname: Li
authors.affiliation: School of Life Sciences, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors.forename: Jingtian
authors.initials: J
authors.lastname: Ni
authors.affiliation: School of Life Sciences, Anhui Agricultural University, Hefei, Anhui 230036, China. Electronic address: xuet@ahau.edu.cn.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000378:metabolism*; D002417:Cattle; D004926:Escherichia coli / Q000235:genetics*; D005260:Female; D015964:Gene Expression Regulation, Bacterial; D006714:Homoserine / Q000031:analogs & derivatives* / Q000378:metabolism; D007783:Lactones / Q000378:metabolism*; D008414:Mastitis, Bovine / Q000382:microbiology; D018440:beta-Lactam Resistance; D047090:beta-Lactams / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D007783:Lactones; C091088:N-octanoylhomoserine lactone; D047090:beta-Lactams; D006714:Homoserine
keywords: Escherichia coli; autoinducer 2 (AI-2) quorum sensing; extended spectrum β-lactamase; mastitis