title: Neurons exhibit Lyz2 promoter activity in vivo: Implications for using LysM-Cre mice in myeloid cell research.
abstract: To characterize LysM-Cre mediated gene targeting in mice, we crossed LysM-Cre mice to two independent reporter-mouse lines (tdTomato or YFP). Surprisingly, we found that more than 90% of cells with LysM-Cre mediated recombination in the brain were neurons, rather than myeloid cells, such as microglia. Hence, by using the LysM-Cre mouse line for conditional knockout approaches, a significant neuronal recombination needs to be considered.
authors.affiliation: Carl-Ludwig Institute of Physiology, Leipzig University, Leipzig, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Orthgiess
authors.affiliation: Institute of Anatomy, Leipzig University, Leipzig, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Gericke
authors.affiliation: Institute of Anatomy, Leipzig University, Leipzig, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Immig
authors.affiliation: Institute of Biochemistry, Leipzig University, Leipzig, Germany.
authors.forename: Angela
authors.initials: A
authors.lastname: Schulz
authors.affiliation: Carl-Ludwig Institute of Physiology, Leipzig University, Leipzig, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Hirrlinger
authors.affiliation: Institute of Anatomy, Leipzig University, Leipzig, Germany.
authors.forename: Ingo
authors.initials: I
authors.lastname: Bechmann
authors.affiliation: Carl-Ludwig Institute of Physiology, Leipzig University, Leipzig, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Eilers
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism; D015870:Gene Expression; D055786:Gene Knockout Techniques; D018390:Gene Targeting; D017930:Genes, Reporter; D059765:Homologous Recombination; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D017628:Microglia / Q000378:metabolism; D009113:Muramidase / Q000235:genetics*; D022423:Myeloid Cells / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D057905:Stem Cell Research; D015533:Transcriptional Activation
chemical_list: D009113:Muramidase; C472450:lysozyme M, mouse
keywords: Brain; LysM-Cre; Lyz2; Microglia; Neuroinflammation; Neurons