title: Rabies direct fluorescent antibody test does not inactivate rabies or eastern equine encephalitis viruses.
abstract: An examination using the routine rabies direct fluorescent antibody test was performed on rabies or Eastern equine encephalitis positive mammalian brain tissue to assess inactivation of the virus. Neither virus was inactivated with acetone fixation nor the routine test, thus laboratory employees should treat all samples as rabies and when appropriate Eastern equine encephalitis positive throughout the whole procedure.
authors.affiliation: Rabies Laboratory, Wadsworth Center, New York State Department of Health, Slingerlands, NY, USA. Electronic address: jodie.jarvis@health.ny.gov.
authors.forename: Jodie A
authors.initials: JA
authors.lastname: Jarvis
authors.affiliation: Arbovirus Laboratory, Wadsworth Center, New York State Department of Health, Slingerlands, NY, USA.
authors.forename: Mary A
authors.initials: MA
authors.lastname: Franke
authors.affiliation: Rabies Laboratory, Wadsworth Center, New York State Department of Health, Slingerlands, NY, USA.
authors.forename: April D
authors.initials: AD
authors.lastname: Davis
mesh_terms: D000096:Acetone / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D000914:Antibodies, Viral / Q000737:chemistry / Q000276:immunology; D001921:Brain / Q000821:virology; D004663:Encephalitis Virus, Eastern Equine / Q000276:immunology / Q000502:physiology*; D020242:Encephalomyelitis, Eastern Equine / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D019085:Fluorescent Antibody Technique, Direct; D006652:Histological Techniques / Q000379:methods; D006736:Horses; D006801:Humans; D011818:Rabies / Q000662:veterinary; D011820:Rabies virus / Q000276:immunology* / Q000502:physiology*; D013194:Staining and Labeling / Q000379:methods / Q000592:standards; D038301:Virus Inactivation
chemical_list: D000914:Antibodies, Viral; D000096:Acetone
keywords: Acetone; Diagnosis; Direct fluorescent antibody test; Eastern equine encephalitis; Inactivation; Rabies