title: Genome-wide identification and expression analysis of the metacaspase gene family in Hevea brasiliensis.
abstract: Metacaspases, a family of cysteine proteases, have been suggested to play important roles in programmed cell death (PCD) during plant development and stress responses. To date, no systematic characterization of this gene family has been reported in rubber tree (Hevea brasiliensis). In the present study, nine metacaspase genes, designated as HbMC1 to HbMC9, were identified from whole-genome sequence of rubber tree. Multiple sequence alignment and phylogenetic analyses suggested that these genes were divided into two types: type I (HbMC1-HBMC7) and type II (HbMC8 and HbMC9). Gene structure analysis demonstrated that type I and type II HbMCs separately contained four and two introns, indicating the conserved exon-intron organization of HbMCs. Quantitative real-time PCR analysis revealed that HbMCs showed distinct expression patterns in different tissues, suggesting the functional diversity of HbMCs in various tissues during development. Most of the HbMCs were regulated by drought, cold, and salt stress, implying their possible functions in regulating abiotic stress-induced cell death. Of the nine HbMCs, HbMC1, HbMC2, HbMC5, and HbMC8 displayed a significantly higher relative transcript accumulation in barks of tapping panel dryness (TPD) trees compared with healthy trees. In addition, the four genes were up-regulated by ethephon (ET) and methyl jasmonate (MeJA), indicating their potential involvement in TPD resulting from ET- or JA-induced PCD. In summary, this work provides valuable information for further functional characterization of HbMC genes in rubber tree.
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China. Electronic address: liuhui8645@163.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China. Electronic address: zizip@163.com.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Deng
authors.affiliation: College of Agriculture, Hainan University, Haikou 570228, China. Electronic address: 1922930134@qq.com.
authors.forename: Jiangshu
authors.initials: J
authors.lastname: Chen
authors.affiliation: CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: wangsen@big.ac.cn.
authors.forename: Sen
authors.initials: S
authors.lastname: Wang
authors.affiliation: CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: haolili@big.ac.cn.
authors.forename: Lili
authors.initials: L
authors.lastname: Hao
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China. Electronic address: djli.rricatas@gmail.com.
authors.forename: Dejun
authors.initials: D
authors.lastname: Li
mesh_terms: D000085:Acetates / Q000494:pharmacology; D000595:Amino Acid Sequence; D020169:Caspases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003080:Cold Temperature; D003517:Cyclopentanes / Q000494:pharmacology; D055864:Droughts; D005030:Ethylenes / Q000494:pharmacology; D005091:Exons / Q000235:genetics; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D028482:Hevea / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D007438:Introns / Q000235:genetics; D007840:Latex / Q000378:metabolism; D005810:Multigene Family; D054883:Oxylipins / Q000494:pharmacology; D010802:Phylogeny; D024301:Plant Bark / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000072417:Protein Domains; D016415:Sequence Alignment; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D005030:Ethylenes; D007840:Latex; D054883:Oxylipins; D010940:Plant Proteins; C072239:methyl jasmonate; C036216:ethylene; D020169:Caspases
keywords: Abiotic stress; Hevea brasiliensis; Metacaspase; Programmed cell death; Tapping panel dryness