title: N-acetylcysteine leads to greater ovarian protection than enoxaparin sodium in a rat ovarian torsion model.
abstract: This study evaluated the effects of N-acetylcysteine (NAC) and enoxaparin on ovarian tissue preservation, ovarian reserve and oxidative damage following ovarian torsion/detorsion injury. Rats were divided into four groups (n = 6/group): control; ischaemia/reperfusion (I/R); I/R + NAC; I/R + enoxaparin. Twenty-four hours after detorsion, ovarian tissues were collected for histopathological analysis and measurement of tissue 8-OHdG, GSH, MDA, MPO and SOD concentrations, as well as pre- and post-operative circulating AMH concentrations. Administration of NAC resulted in more pre-antral follicles compared with enoxaparin treatment and haemorrhage and follicle cell degeneration were more pronounced in I/R + enoxaparin group than I/R + NAC group. Both NAC and enoxaparin led to a significant reduction in ovarian tissue 8-OHdG (P = 0.004 and P = 0.01, respectively) and MPO (P = 0.013 and P = 0.023, respectively) concentrations compared with I/R group, indicating a protective effect against I/R oxidative damage. Only NAC-treated animals showed a significant increase in GSH and SOD concentrations and decrease in MDA concentrations compared with I/R group (P = 0.007, P = 0.024 and P = 0.026, respectively). These results indicate that NAC is more effective than enoxaparin in minimizing ovarian damage and preserving ovarian reserve following ovarian torsion.
authors.affiliation: Department of Obstetrics and Gynecology, Kartal Dr. Lutfi Kirdar Education and Research Hospital, Istanbul, Turkey. Electronic address: gulcinsahinmd@gmail.com.
authors.forename: Gulcin
authors.initials: G
authors.lastname: Sahin Ersoy
authors.affiliation: Department of Obstetrics and Gynecology, Zeynep Kamil Education and Research Hospital, Istanbul, Turkey.
authors.forename: Meryem
authors.initials: M
authors.lastname: Eken
authors.affiliation: Division of Reproductive Endocrinology and Infertility, Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, CT, USA.
authors.forename: Reshef
authors.initials: R
authors.lastname: Tal
authors.affiliation: Department of Obstetrics and Gynecology, Tepecik Education and Research Hospital, Izmir, Turkey.
authors.forename: Deniz
authors.initials: D
authors.lastname: Oztekin
authors.affiliation: Department of Obstetrics and Gynecology, Zeynep Kamil Education and Research Hospital, Istanbul, Turkey.
authors.forename: Belgin
authors.initials: B
authors.lastname: Devranoglu
authors.affiliation: Department of Pathology, Zeynep Kamil Education and Research Hospital, Istanbul, Turkey.
authors.forename: Ecmel
authors.initials: E
authors.lastname: Isik Kaygusuz
authors.affiliation: Department of Biochemistry, Cumhuriyet University, Faculty of Pharmacy, Sivas, Turkey.
authors.forename: Ozge
authors.initials: O
authors.lastname: Cevik
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000111:Acetylcysteine / Q000627:therapeutic use*; D000818:Animals; D054304:Anti-Mullerian Hormone / Q000097:blood; D000975:Antioxidants / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D017984:Enoxaparin / Q000627:therapeutic use*; D005260:Female; D005978:Glutathione / Q000378:metabolism; D020287:In Situ Nick-End Labeling; D008315:Malondialdehyde / Q000378:metabolism; D010049:Ovarian Diseases / Q000188:drug therapy*; D006080:Ovarian Follicle / Q000187:drug effects; D010053:Ovary / Q000187:drug effects*; D018384:Oxidative Stress; D009195:Peroxidase / Q000378:metabolism; D010865:Pilot Projects; D051381:Rats; D017208:Rats, Wistar; D027724:Reproductive Techniques, Assisted; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D017984:Enoxaparin; D008315:Malondialdehyde; D054304:Anti-Mullerian Hormone; D000080242:8-Hydroxy-2'-Deoxyguanosine; D009195:Peroxidase; D013482:Superoxide Dismutase; D003849:Deoxyguanosine; D005978:Glutathione; D000111:Acetylcysteine
keywords: N-acetylcysteine; anti-Müllerian hormone; enoxaparin sodium; ovarian ischaemia-reperfusion; ovarian reserve; torsion