title: Compositional Analysis of Biofilms Formed by Staphylococcus aureus Isolated from Food Sources.
abstract: Sixteen Staphylococcus aureus isolates originating from foods (eight from dairy products, five from fish and fish products and three from meat and meat products) were evaluated regarding their biofilms formation ability. Six strains (E2, E6, E8, E10, E16, and E23) distinguished as strong biofilm formers, either in standard Tryptic Soy Broth or in Tryptic Soy Broth supplemented with 0.4% glucose or with 4% NaCl. The composition of the biofilms formed by these S. aureus strains on polystyrene surfaces was first inferred using enzymatic and chemical treatments. Later on, biofilms were characterized by confocal laser scanning microscope (CLSM). Our experiments proved that protein-based matrices are of prime importance for the structure of biofilms formed by S. aureus strains isolated from food sources. These biofilm matrix compositions are similar to those put into evidence for coagulase negative staphylococci. This is a new finding having in view that scientific literature mentions exopolysaccharide abundance in biofilms produced by clinical isolates and food processing environment isolates of S. aureus.
authors.affiliation: Faculty of Food Science and Engineering, Dunarea de Jos University of GalatiGalati, Romania; Centre of Biological Engineering, Universidade do MinhoBraga, Portugal.
authors.forename: Elena-Alexandra
authors.initials: EA
authors.lastname: Oniciuc
authors.affiliation: Centre of Biological Engineering, Universidade do Minho Braga, Portugal.
authors.forename: Nuno
authors.initials: N
authors.lastname: Cerca
authors.affiliation: Faculty of Food Science and Engineering, Dunarea de Jos University of Galati Galati, Romania.
authors.forename: Anca I
authors.initials: AI
authors.lastname: Nicolau
keywords: CLSM; Staphylococcus aureus; biofilm; exopolysaccharide; food; protein