title: Potential Role of S100A8 in Cutaneous Squamous Cell Carcinoma Differentiation.
abstract: BACKGROUND
  S100A8 is differentially expressed in various cell types and is associated with a number of malignant disorders. S100A8 may affect tumor biology. However, its role in cutaneous squamous cell carcinoma (SCC) is not well established.
  
  
  OBJECTIVE
  This study aims to investigate the relationship between S100A8 and cutaneous SCC development.
  
  
  METHODS
  We performed immunohistochemical staining to detect S100A8 expression in facial skin specimens of premalignant actinic keratosis (AK), malignant SCC, and normal tissues. In addition, we utilized postconfluence and high calcium-induced differentiation in a culture system model. Furthermore, we constructed a recombinant adenovirus expressing GFP-tagged S100A8 to investigate the role of S100A8 in SCC cell differentiation.
  
  
  RESULTS
  S100A8 was significantly overexpressed in human cutaneous SCC compared to that in normal and AK tissues. S100A8 was gradually upregulated in SCC cells in a post-confluence-induced differentiation model. Overexpression of S100A8 in SCC cells induced by adenoviral transduction led to increased expression levels of differentiation markers, such as loricrin, involucrin, and filaggrin. S100A8 overexpression also increased loricrin and involucrin luciferase activity.
  
  
  CONCLUSIONS
  S100A8 regulates cutaneous SCC differentiation and induces well-differentiated SCC formation in skin.
authors.affiliation: Department of Dermatology and Research Institute for Medical Sciences, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Jung-Min
authors.initials: JM
authors.lastname: Shin
authors.affiliation: Department of Dermatology and Research Institute for Medical Sciences, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: In-Kyu
authors.initials: IK
authors.lastname: Chang
authors.affiliation: Department of Anatomy, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Young-Ho
authors.initials: YH
authors.lastname: Lee
authors.affiliation: Department of Pathology, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Min-Kyung
authors.initials: MK
authors.lastname: Yeo
authors.affiliation: Department of Pathology, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Jin-Man
authors.initials: JM
authors.lastname: Kim
authors.affiliation: Department of Dermatology and Research Institute for Medical Sciences, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Kyung-Cheol
authors.initials: KC
authors.lastname: Sohn
authors.affiliation: Department of Dermatology and Research Institute for Medical Sciences, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Myung
authors.initials: M
authors.lastname: Im
authors.affiliation: Department of Dermatology and Research Institute for Medical Sciences, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Young-Joon
authors.initials: YJ
authors.lastname: Seo
authors.affiliation: Department of Dermatology and Research Institute for Medical Sciences, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Chang-Deok
authors.initials: CD
authors.lastname: Kim
authors.affiliation: Department of Dermatology and Research Institute for Medical Sciences, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Jeung-Hoon
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Dermatology and Research Institute for Medical Sciences, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Young
authors.initials: Y
authors.lastname: Lee
keywords: Actinic keratosis; Differentiation; S100; S100A8; Squamous cell carcinoma