title: Effects of "Danzhi Decoction" on Chronic Pelvic Pain, Hemodynamics, and Proinflammatory Factors in the Murine Model of Sequelae of Pelvic Inflammatory Disease.
abstract: Objective. To evaluate the effect of Danzhi decoction (DZD) on chronic pelvic pain (CPP), hemodynamics, and proinflammatory factors of sequelae of pelvic inflammatory diseases (SPID) in murine model. Methods. SPID mice were randomly treated with high-dose DZD, mid-dose DZD, low-dose DZD, aspirin, and vehicle for 3 estrous circles. The Mouse Grimace Scale (MGS) was performed to evaluate CPP; blood flows of the upper genital tract, pelvic wall, and mesentery were used to assess hemodynamics in SPID mice; expressions of vascular endothelial growth factor (VEGF), angiopoietin-2 (Ang-2), and osteopontin (OPN) were measured by Western blot and immunochemistry. Results. Treatment with dose-dependent DZD significantly decreased the MGS scores, accelerated blood flows of the pelvis, and reduced expressions of VEGF, Ang-2, and OPN in the upper genital tract. Conclusions and Discussions. DZD was effective in relieving CPP and improving hemodynamics of the pelvic blood-stasis microenvironment in SPID mice. There was a relationship between CPP and the pelvic blood-stasis microenvironment. Furthermore, DZD might play a positive role in the anti-inflammatory process.
authors.affiliation: Beijing University of Traditional Chinese Medicine, North 3rd Ring Road No. 11 School Range, Chaoyang District, Beijing 100029, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Bu
authors.affiliation: Department of Gynecology, Dongfang Hospital of Beijing University of Traditional Chinese Medicine, No. 6 Fangxingyuan 1 Qu, Fengtai District, Beijing 100078, China.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Gynecology, Dongfang Hospital of Beijing University of Traditional Chinese Medicine, No. 6 Fangxingyuan 1 Qu, Fengtai District, Beijing 100078, China.
authors.forename: Qiudan
authors.initials: Q
authors.lastname: Lu
authors.affiliation: Department of Gynecology, Dongfang Hospital of Beijing University of Traditional Chinese Medicine, No. 6 Fangxingyuan 1 Qu, Fengtai District, Beijing 100078, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Jin