title: The preparation and characterization of nitric oxide releasing silicone rubber materials impregnated withS-nitroso-tert-dodecyl mercaptan.
abstract: Recently, considerable research efforts have focused on increasing the biocompatibility a nd bactericidal activity of biomedical polymeric devices (e.g., catheters, etc.) through incorporation of nitric oxide (NO) releasing molecules. NO is an important endogenous molecule that is well known for enhancing blood flow via its vasodilatory activity, but it also exhibits potent antithrombotic and antimicrobial properties. In this work, we demonstrate that silicone rubber tubing can be impregnated with a tertiaryS-nitrosothiol (RSNO),S-nitroso-tert-dodecylmercaptan, via a simple solvent swelling method. We further characterize the NO release and RSNO leaching from the tubing over time via use of chemiluminescence and UV/Vis spectroscopy, respectively. The tubing is shown to maintain an NO flux above the physiological levels released by endothelial cells, 0.5-4.0 × 10-10molcm-2min-1, for more than 3 weeks while stored at 37 °C and exhibit minimal leaching. Finally, the RSNO impregnated tu bing exhibits significant antimicrobial activity over a 21 d period (vs. controls) during incubation in a CDC bioreactor after inoculation of media withS. aureusbacteria. The use of such lipophilic RSNO impregnated silicone rubber tubing could dramatically reduce the risk of catheter-related infections, which are a common problem associated with placement of intravascular or urinary catheters.
authors.affiliation: Department of Chemistry, University of Michigan, Ann Arbor, MI 48109.
authors.forename: Alex R
authors.initials: AR
authors.lastname: Ketchum
authors.affiliation: Department of Chemistry, University of Michigan, Ann Arbor, MI 48109.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Kappler
authors.affiliation: Department of Environmental Health Sciences, University of Michigan School of Public Health, Ann Arbor, MI 48109.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Environmental Health Sciences, University of Michigan School of Public Health, Ann Arbor, MI 48109.
authors.forename: Chuanwu
authors.initials: C
authors.lastname: Xi
authors.affiliation: Department of Chemistry, University of Michigan, Ann Arbor, MI 48109.
authors.forename: Mark E
authors.initials: ME
authors.lastname: Meyerhoff