title: Immunomodulatory effect of baculovirus in chickens: How it modifies the immune response against infectious bursal disease virus.
abstract: Several reports have shown that baculoviruses (BVs) have strong adjuvant properties on the mammalian immune system. Recent studies of our group demonstrated the ability of BV to stimulate the innate immunity in chickens. In this investigation, we aimed to assess the potential antiviral effect of BV given both, before and after infectious bursal disease virus (IBDV). In the first case, specific pathogen free chickens were intravenously inoculated with 5 × 10(7) pfu of Autographa californica nuclear polyhedrosis virus and 3 h later were orally administered 2.5 × 10(5) egg infectious doses 50 of IBDV. In the second case, chickens received IBDV 3 h before BV inoculation. Five days later, chickens were bled and euthanized. RNA from the bursa was analyzed for cytokine production. Also, bursae were used for virus recovery, and processed for lymphocyte isolation. The results showed that the administration of BV 3 h after the inoculation with IBDV produced important changes in the effect that IBDV causes in the bursa. BV reduced the infiltration of T lymphocytes, decreased the expression pattern of IL-6 and IFN-γ and inhibited IBDV replication. The results herein presented demonstrate that this Lepidopteran virus shows antiviral activity in chickens under experimental conditions. Investigations under field conditions have to be done to probe this strategy as a valuable sanitary tool for the treatment and prevention of chicken diseases.
authors.affiliation: Instituto de Biotecnología, CICVyA, INTA, Castelar, Cc 25, B1712WAA, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rivadavia, 1917, C1033AAJ, Ciudad de Buenos Aires, Argentina. Electronic address: chimenozoth.silvina@inta.gob.ar.
authors.forename: Silvina
authors.initials: S
authors.lastname: Chimeno Zoth
authors.affiliation: Instituto de Biotecnología, CICVyA, INTA, Castelar, Cc 25, B1712WAA, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rivadavia, 1917, C1033AAJ, Ciudad de Buenos Aires, Argentina.
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Carballeda
authors.affiliation: Instituto de Biotecnología, CICVyA, INTA, Castelar, Cc 25, B1712WAA, Buenos Aires, Argentina.
authors.forename: María José
authors.initials: MJ
authors.lastname: Gravisaco
authors.affiliation: Instituto de Biotecnología, CICVyA, INTA, Castelar, Cc 25, B1712WAA, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rivadavia, 1917, C1033AAJ, Ciudad de Buenos Aires, Argentina.
authors.forename: María Soledad
authors.initials: MS
authors.lastname: Lucero
authors.affiliation: Instituto de Biotecnología, CICVyA, INTA, Castelar, Cc 25, B1712WAA, Buenos Aires, Argentina; Agencia Nacional de Promoción Científica y Técnica (ANPCyT), Godoy Cruz, 2370, C1425FQD, Ciudad de Buenos Aires, Argentina.
authors.forename: Matías
authors.initials: M
authors.lastname: Richetta
authors.affiliation: Instituto de Biotecnología, CICVyA, INTA, Castelar, Cc 25, B1712WAA, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rivadavia, 1917, C1033AAJ, Ciudad de Buenos Aires, Argentina.
authors.forename: Evangelina
authors.initials: E
authors.lastname: Gómez
authors.affiliation: Instituto de Biotecnología, CICVyA, INTA, Castelar, Cc 25, B1712WAA, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rivadavia, 1917, C1033AAJ, Ciudad de Buenos Aires, Argentina. Electronic address: berinstein.analia@inta.gob.ar.
authors.forename: Analía
authors.initials: A
authors.lastname: Berinstein
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000914:Antibodies, Viral; D016367:Baculoviridae / Q000276:immunology* / Q000502:physiology; D018175:Birnaviridae Infections / Q000276:immunology / Q000628:therapy / Q000662:veterinary* / Q000821:virology; D002645:Chickens / Q000276:immunology* / Q000821:virology; D007113:Immunity, Innate; D056747:Immunomodulation; D007243:Infectious bursal disease virus / Q000276:immunology*; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology; D018655:Lymphocyte Count; D010063:Ovum / Q000821:virology; D011201:Poultry Diseases / Q000276:immunology* / Q000517:prevention & control / Q000628:therapy / Q000821:virology; D013047:Specific Pathogen-Free Organisms; D013601:T-Lymphocytes; D014779:Virus Replication
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D015850:Interleukin-6; D007371:Interferon-gamma
keywords: Avian innate immunity; Baculovirus; Infectious bursal disease virus