title: A Bioinformatics Method for the Design of Live Attenuated Virus Vaccine Utilizing Host MicroRNA Response Elements.
abstract: The host microRNA machinery has been employed to control viral replication. To improve safety for live attenuated virus vaccines, the binding sites of the host microRNAs, so-called microRNA response elements (MREs), were incorporated into the virus sequences. These MREs were typically designed for a specific host microRNA and virus sequence with the effectiveness evaluated by experimental trials. Here, we describe a computational flow that can be used to simultaneously design and prioritize the effective MREs in large-scale.
authors.affiliation: Department of Computer Engineering, Faculty of Engineering, Chulalongkorn University, Phaya Thai Road, Wangmai, Pathumwan, Bangkok, 10330, Thailand. duangdao.w@chula.ac.th.
authors.forename: Duangdao
authors.initials: D
authors.lastname: Wichadakul
mesh_terms: D019295:Computational Biology / Q000379:methods*; D005818:Genetic Engineering / Q000379:methods*; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology; D007252:Influenza Vaccines / Q000235:genetics* / Q000276:immunology; D035683:MicroRNAs / Q000235:genetics*; D020218:Response Elements / Q000235:genetics*; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology
chemical_list: D007252:Influenza Vaccines; D035683:MicroRNAs; D014613:Vaccines, Attenuated
keywords: Bioinformatics vaccine; Host microRNA response elements (MREs); Live attenuated virus vaccine; Vaccine design