title: Investigation of age-related changes in the expression of aquaporin-1 and aquaporin-5 in the salivary glands of mice.
abstract: CONCLUSIONS
  The increased AQP5 expression associated with ageing in glands, which mainly secreted a serous solution, suggests a compensation for the decreased amount of saliva secretion associated with age progression.
  
  
  OBJECTIVE
  To investigate the change in aquaporin-1 (AQP1) and aquaporin-5 (AQP5) expression in the salivary glands in young and elder mice.
  
  
  METHODS
  Twelve female mice from the Balb/C genus (30-50 g) were used. The mice were separated into two groups: Group I had 2-month-old mice and Group II had 18-month-old mice. Salivary glands (glandula parotidea, glandula sublungualis, glandula submaxillaris) were excised and examined immunohistochemically and histopathologically. AQP1 and AQP5 expression of young and elder mice was evaluated using the H-score. A p-value less than 0.05 was considered statistically significant.
  
  
  RESULTS
  Upon histopathological examination, the acini of glands were found to be atrophic in elder mice. The number and diameter of intercalated ducts were increased. Indeed, the amount of adipose tissue in the gland was increased. Upon immunohistochemical examination, both AQP1 and AQP5 levels in sublingual glands of elder mice were increased (p < 0.01 and p < 0.001, respectively). However, only AQP5 levels were increased in the parotid gland of elder mice (p < 0.01).
authors.affiliation: a Department of Otorhinolaryngology, Faculty of Medicine , Gaziosmanpaşa University , Tokat , Turkey ;
authors.forename: Emrah
authors.initials: E
authors.lastname: Sapmaz
authors.affiliation: b Department of Anatomy, Faculty of Medicine , Gaziosmanpaşa University , Tokat , Turkey ;
authors.forename: Murat
authors.initials: M
authors.lastname: Uysal
authors.affiliation: c Department of Oral and Maxillofacial Surgery, Faculty of Dentistry , Gaziosmanpaşa University , Tokat , Turkey ;
authors.forename: Mehmet Kemal
authors.initials: MK
authors.lastname: Tumer
authors.affiliation: b Department of Anatomy, Faculty of Medicine , Gaziosmanpaşa University , Tokat , Turkey ;
authors.forename: Hilal Irmak
authors.initials: HI
authors.lastname: Sapmaz
authors.affiliation: a Department of Otorhinolaryngology, Faculty of Medicine , Gaziosmanpaşa University , Tokat , Turkey ;
authors.forename: Battal Tahsin
authors.initials: BT
authors.lastname: Somuk
authors.affiliation: d Department of Pathology, Faculty of Medicine , Gaziosmanpaşa University , Tokat , Turkey.
authors.forename: Akgul
authors.initials: A
authors.lastname: Arici
authors.affiliation: b Department of Anatomy, Faculty of Medicine , Gaziosmanpaşa University , Tokat , Turkey ;
authors.forename: Ufuk
authors.initials: U
authors.lastname: Tas
mesh_terms: D000375:Aging / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D051398:Aquaporin 1 / Q000378:metabolism*; D051402:Aquaporin 5 / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D008807:Mice, Inbred BALB C; D012469:Salivary Glands / Q000378:metabolism* / Q000473:pathology
chemical_list: C496021:Aqp1 protein, mouse; C496038:Aqp5 protein, mouse; D051402:Aquaporin 5; D051398:Aquaporin 1
keywords: Ageing; aquaporins; mice; salivary glands