title: Effects of IL-10 and glucose on expression of OPG and RANKL in human periodontal ligament fibroblasts.
abstract: The effects of interleukin-10 (IL-10) and glucose on mRNA and protein expression of osteoprotegerin (OPG), and its ligand, receptor activator of nuclear factor-κB ligand (RANKL), were investigated in human periodontal ligament fibroblasts (HPDLFs). Primary HPDLFs were treated with different concentrations of IL-10 (0, 1, 10, 25, 50, and 100 ng/mL) or glucose (0, 5.5, 10, 20, 30, and 40 mmol/L). Changes in mRNA and protein expression were examined using the reverse-transcription polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. After IL-10 treatment, mRNA and protein levels of OPG were increased, while mRNA and protein levels of RANKL were decreased (P<0.05), both in a concentration-dependent manner. Glucose stimulation had the opposite concentration-dependent effect to that of IL-10 on OPG and RANKL expression. IL-10 upregulated OPG expression and downregulated RANKL expression, whereas high glucose upregulated RANKL and downregulated OPG in HDPLFs. Abnormal levels of IL-10 and glucose may contribute to the pathogenesis of periodontal disease.
authors.affiliation: Stomatology Hospital, Xi'an Jiaotong University College of Medicine, Xi'an, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Wuxi Mental Health Center of, Nanjing Medical University, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Stomatology Hospital, Xi'an Jiaotong University College of Medicine, Xi'an, China.
authors.forename: G Z
authors.initials: GZ
authors.lastname: Rao
authors.affiliation: Stomatology Hospital, Xi'an Jiaotong University College of Medicine, Xi'an, China.
authors.forename: D
authors.initials: D
authors.lastname: Miao
mesh_terms: D000704:Analysis of Variance; D015153:Blotting, Western; D002478:Cells, Cultured; D015536:Down-Regulation; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology*; D053244:Osteoprotegerin / Q000378:metabolism*; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*; D053245:RANK Ligand / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D053244:Osteoprotegerin; D053245:RANK Ligand; D012333:RNA, Messenger; D016753:Interleukin-10; D005947:Glucose