title: Applications in image-based profiling of perturbations.
abstract: A dramatic shift has occurred in how biologists use microscopy images. Whether experiments are small-scale or high-throughput, automatically quantifying biological properties in images is now widespread. We see yet another revolution under way: a transition towards using automated image analysis to not only identify phenotypes a biologist specifically seeks to measure ('screening') but also as an unbiased and sensitive tool to capture a wide variety of subtle features of cell (or organism) state ('profiling'). Mapping similarities among samples using image-based (morphological) profiling has tremendous potential to transform drug discovery, functional genomics, and basic biological research. Applications include target identification, lead hopping, library enrichment, functionally annotating genes/alleles, and identifying small molecule modulators of gene activity and disease-specific phenotypes.
authors.affiliation: Imaging Platform of the Broad Institute of Harvard and Massachusetts Institute of Technology, 415 Main Street, Cambridge, MA, USA; Fundación Universitaria Konrad Lorenz, Bogotá, Colombia.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Caicedo
authors.affiliation: Imaging Platform of the Broad Institute of Harvard and Massachusetts Institute of Technology, 415 Main Street, Cambridge, MA, USA.
authors.forename: Shantanu
authors.initials: S
authors.lastname: Singh
authors.affiliation: Imaging Platform of the Broad Institute of Harvard and Massachusetts Institute of Technology, 415 Main Street, Cambridge, MA, USA. Electronic address: anne@broadinstitute.org.
authors.forename: Anne E
authors.initials: AE
authors.lastname: Carpenter
mesh_terms: D000818:Animals; D002468:Cell Physiological Phenomena; D055808:Drug Discovery / Q000379:methods*; D020869:Gene Expression Profiling; D023281:Genomics / Q000379:methods; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D010641:Phenotype; D020543:Proteome / Q000032:analysis*
chemical_list: D020543:Proteome