title: Effect of supplementation of butylated hydroxytoluene on post-thaw sperm viability, motility and membrane integrity of Hariana bulls.
abstract: OBJECTIVE
  This study was aimed to see the beneficial effect of butylated hydroxytoluene (BHT) as a semen additive of Hariana bull semen.
  
  
  METHODS
  The study was carried out in Hariana bulls. Twenty-four ejaculates from two bulls were used for this study. Each ejaculate was extended with standard glycerolated egg yolk tris extender and supplemented with BHT at two concentrations as 0.5 mM (T1) and 1.0 mM (T2). After dilution, equilibration and 24 h of cryopreservation, the samples were analyzed for progressive motility, sperm viability and membrane integrity.
  
  
  RESULTS
  Progressive motility, sperm viability and sperm membrane integrity were significantly (p<0.05) increased in the samples fortified with BHT as compared to the control during the process of cryopreservation and thawing. The BHT concentration of 1 mM revealed better results as compared to 0.5 mM.
  
  
  CONCLUSIONS
  Addition of 1.0 mM BHT was found better in cryopreservation of Hariana bull semen compared to 0.5 mM BHT and control samples. The addition of BHT has improved the sperm quality by acting as an antioxidant thereby reducing the lipid peroxidation of the sperms.
authors.affiliation: Department of Veterinary Obstetrics & Gynaecology, College of Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura - 281 001, Uttar Pradesh, India.
authors.forename: Akhil
authors.initials: A
authors.lastname: Patel
authors.affiliation: Department of Veterinary Obstetrics & Gynaecology, College of Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura - 281 001, Uttar Pradesh, India.
authors.forename: Atul
authors.initials: A
authors.lastname: Saxena
authors.affiliation: Department of Veterinary Physiology, College of Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura - 281 001, Uttar Pradesh, India.
authors.forename: Dilip Kumar
authors.initials: DK
authors.lastname: Swain
authors.affiliation: Department of Veterinary Obstetrics & Gynaecology, College of Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura - 281 001, Uttar Pradesh, India.
authors.forename: Dushyant
authors.initials: D
authors.lastname: Yadav
authors.affiliation: Department of Veterinary Obstetrics & Gynaecology, College of Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura - 281 001, Uttar Pradesh, India.
authors.forename: Sanjay Singh
authors.initials: SS
authors.lastname: Yadav
authors.affiliation: Department of Veterinary Obstetrics & Gynaecology, College of Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura - 281 001, Uttar Pradesh, India.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Department of Veterinary Obstetrics & Gynaecology, College of Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura - 281 001, Uttar Pradesh, India.
authors.forename: Anuj
authors.initials: A
authors.lastname: Kumar
keywords: Hariana bull; antioxidant; butylated hydroxytoluene; membrane integrity; sperm; viability