title: Identification of a functional SNP in the 3'-UTR of caprine MTHFR gene that is associated with milk protein levels.
abstract: Xinong Saanen (n = 305) and Guanzhong (n = 317) dairy goats were used to detect SNPs in the caprine MTHFR 3'-UTR by DNA sequencing. One novel SNP (c.*2494G>A) was identified in the said region. Individuals with the AA genotype had greater milk protein levels than did those with the GG genotype at the c.*2494 G>A locus in both dairy goat breeds (P < 0.05). Functional assays indicated that the MTHFR:c.2494G>A substitution could increase the binding activity of bta-miR-370 with the MTHFR 3'-UTR. In addition, we observed a significant increase in the MTHFR protein level of AA carriers relative to that of GG carriers. These altered levels of MTHFR protein may account for the association of the SNP with milk protein level.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, P.R. China.
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: An
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, P.R. China.
authors.forename: Yuxuan
authors.initials: Y
authors.lastname: Song
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, P.R. China.
authors.forename: Jinxing
authors.initials: J
authors.lastname: Hou
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, P.R. China.
authors.forename: Shan
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, P.R. China.
authors.forename: Kexin
authors.initials: K
authors.lastname: Gao
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, P.R. China.
authors.forename: Binyun
authors.initials: B
authors.lastname: Cao
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D001947:Breeding; D005260:Female; D005838:Genotype; D006041:Goats / Q000235:genetics*; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008892:Milk / Q000737:chemistry; D008894:Milk Proteins / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
chemical_list: D020413:3' Untranslated Regions; D008894:Milk Proteins; D042965:Methylenetetrahydrofolate Reductase (NADPH2)
keywords: DNA sequencing; breeding; gene mutation; miRNAs