title: Glypican-4 gene polymorphism (rs1048369) and susceptibility to Epstein-Barr virus-associated and -negative gastric carcinoma.
abstract: Gastric cancer (GC) is one of the most common malignant tumors in China and single nucleotide polymorphisms (SNPs) have been found to be highly related to GC carcinogenesis. Glypican-4 (GPC4), a member of the heparan sulphate proteoglycan family, plays an important role in the regulation of cell growth and differentiation. However, little is known about polymorphisms of GPC4 gene and their associated susceptibility to GC, especially to Epstein-Barr virus-associated GC (EBVaGC). Here we studied the GPC4 polymorphism (rs1048369) in GC individuals, especially those with EBVaGC, and we explored an association between the GPC4 gene polymorphism (rs1048369) and susceptibility to EBVaGC and Epstein-Barr virus-negative GC (EBVnGC) in a population from Northern China.
  
  
  
  The GPC4 gene polymorphism (rs1048369) was detected in 54 cases of EBVaGC and 73 cases of EBVnGC using polymerase chain reaction (PCR). One hundred and seven peripheral blood samples from healthy individuals were also measured as a control group.
  
  
  
  There were significant differences in both the genotype and allelic frequency of GPC4 gene (rs1048369) between the EBVaGC and EBVnGC patients. Meanwhile, the distribution of genotype and allelic frequency of GPC4 (rs1048369) differed between EBVaGC and control groups. Distribution of the GPC4 genotype also revealed differences between EBVnGC and control groups, no significant differences in the allelic frequency of the GPC4 gene (rs1048369) were observed. The frequency of the T allele in EBVaGC group was significantly higher than that in control and EBVnGC groups.
  
  
  
  The GPC4 gene polymorphism and the allele of GPC4 are both associated with susceptibility to EBVaGC. The T allele of GPC4 may represent a risk factor for EBVaGC.
authors.affiliation: Department of Medical Microbiology, Qingdao University Medical College, Qingdao, China.
authors.forename: Danrui
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Department of Blood Transfusion, The Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Pathology, The Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Medical Microbiology, Qingdao University Medical College, Qingdao, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Medical Microbiology, Qingdao University Medical College, Qingdao, China.
authors.forename: Song
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Medical Microbiology, Qingdao University Medical College, Qingdao, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Kuang
authors.affiliation: Department of Medical Microbiology, Qingdao University Medical College, Qingdao, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Shu
authors.affiliation: Department of Medical Microbiology, Qingdao University Medical College, Qingdao, China. Electronic address: qdluobing@163.com.
authors.forename: Bing
authors.initials: B
authors.lastname: Luo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D001483:Base Sequence; D002277:Carcinoma / Q000150:complications / Q000235:genetics* / Q000276:immunology / Q000821:virology; D016022:Case-Control Studies; D020031:Epstein-Barr Virus Infections / Q000150:complications / Q000235:genetics* / Q000276:immunology / Q000821:virology; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D053673:Glypicans / Q000235:genetics* / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity; D054884:Host-Pathogen Interactions; D006801:Humans; D008297:Male; D008875:Middle Aged; D008957:Models, Genetic; D020641:Polymorphism, Single Nucleotide; D013274:Stomach Neoplasms / Q000150:complications / Q000235:genetics* / Q000276:immunology / Q000821:virology
chemical_list: C492044:GPC4 protein, human; D053673:Glypicans
keywords: Epstein-Barr virus-associated gastric carcinoma; Epstein-Barr virus-negative gastric carcinoma; GPC4; Gene polymorphism