title: Towards efficient bioethanol production from agricultural and forestry residues: Exploration of unique natural microorganisms in combination with advanced strain engineering.
abstract: Production of fuel ethanol from lignocellulosic feedstocks such as agricultural and forestry residues is receiving increasing attention due to the unsustainable supply of fossil fuels. Three key challenges include high cellulase production cost, toxicity of the cellulosic hydrolysate to microbial strains, and poor ability of fermenting microorganisms to utilize certain fermentable sugars in the hydrolysate. In this article, studies on searching of natural microbial strains for production of unique cellulase for biorefinery of agricultural and forestry wastes, as well as development of strains for improved cellulase production were reviewed. In addition, progress in the construction of yeast strains with improved stress tolerance and the capability to fully utilize xylose and glucose in the cellulosic hydrolysate was also summarized. With the superior microbial strains for high titer cellulase production and efficient utilization of all fermentable sugars in the hydrolysate, economic biofuels production from agricultural residues and forestry wastes can be realized.
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: xqzhao@sjtu.edu.cn.
authors.forename: Xinqing
authors.initials: X
authors.lastname: Zhao
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Xiong
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China; School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
authors.forename: Fengwu
authors.initials: F
authors.lastname: Bai
mesh_terms: D000383:Agriculture; D056804:Biofuels / Q000382:microbiology; D001709:Biotechnology; D002480:Cellulase / Q000096:biosynthesis*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D016468:Forestry; D007218:Industrial Microbiology / Q000379:methods*; D007220:Industrial Waste; D008031:Lignin / Q000378:metabolism; D060847:Metabolic Engineering / Q000379:methods*; D012441:Saccharomyces cerevisiae
chemical_list: D056804:Biofuels; D007220:Industrial Waste; C036909:lignocellulose; D000431:Ethanol; D008031:Lignin; D002480:Cellulase
keywords: Agricultural residues; Cellulase; Fuel ethanol; Saccharomyces cerevisiae; Stress tolerance; Xylose