title: Differences in seminal plasma and spermatozoa antioxidative systems and seminal plasma lipid and protein levels among boar breeds and hybrid genetic traits.
abstract: The objectives of this study were to determine the influence of breed and hybrid genetic traits of boars on lipid and protein concentrations and antioxidative system variables in seminal plasma (SP) and spermatozoa and their correlations with semen quality variables. Semen samples from 27 boars: Swedish Landraces (SL), German Landraces (GL), Large Whites (LW), Pietrains (P) and Pig Improvement Company hybrids (PIC-hybrid), aged from 1.5 to 3 years old, were collected. SP was spectrophotometrically analyzed to determine total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), triacylglycerol (TAG), total protein (TP), albumin, and zinc concentrations. The antioxidative system in SP and spermatozoa was established spectrophotometrically by determining total antioxidative status (TAS), total superoxide dismutase (TSOD) and glutathione peroxidase (GSH-Px) parameters, as well as copper-zinc superoxide dismutase (CuZnSOD) and manganese superoxide dismutase (MnSOD) activity in spermatozoa. The hybrid boars had higher (P<0.05) SP concentrations of: TC, LDL-C and TAG than P and GL; HDL-C than P, GL and SL; and TP than P and LW. PIC-hybrid had lower values (P<0.05) in spermatozoa of: TAS and CuZnSOD than SL; TSOD and GSH-Px than SL and P; and MnSOD than SL and LW. Differences in SP and spermatozoa antioxidative system variables and the significant differences in SP protein and lipid variables exist among boars of different breeds and hybrid. Novel data and observed differences in semen variables among boar breeds and hybrids and their correlations with semen quality parameters in this study could contribute to better assessment of boar semen quality.
authors.affiliation: Faculty of Veterinary Medicine, University of Zagreb, Croatia.
authors.forename: Ivona
authors.initials: I
authors.lastname: Žura Žaja
authors.affiliation: Faculty of Veterinary Medicine, University of Zagreb, Croatia. Electronic address: smarko@vef.hr.
authors.forename: Marko
authors.initials: M
authors.lastname: Samardžija
authors.affiliation: Faculty of Veterinary Medicine, University of Zagreb, Croatia.
authors.forename: Silvijo
authors.initials: S
authors.lastname: Vince
authors.affiliation: Faculty of Veterinary Medicine, University of Zagreb, Croatia.
authors.forename: Marinko
authors.initials: M
authors.lastname: Vilić
authors.affiliation: Center for Reproduction and Animal Breeding of Croatia d.o.o., Zagreb, Croatia.
authors.forename: Ivanka
authors.initials: I
authors.lastname: Majić-Balić
authors.affiliation: Veterinary Practice Đurđevac, Croatia.
authors.forename: Dražen
authors.initials: D
authors.lastname: Đuričić
authors.affiliation: Faculty of Veterinary Medicine, University of Zagreb, Croatia.
authors.forename: Suzana
authors.initials: S
authors.lastname: Milinković-Tur
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001947:Breeding; D006824:Hybridization, Genetic; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry*; D008297:Male; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D012661:Semen / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*; D013552:Swine / Q000235:genetics / Q000502:physiology*
chemical_list: D000975:Antioxidants; D008055:Lipids; D011506:Proteins
keywords: Antioxidative system indicators; Biochemical variables; Boar; Seminal plasma; Spermatozoa