title: Performance of MALDI-TOF MS platforms for fungal identification.
abstract: Matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-TOF MS) is increasingly used by clinical microbiology laboratories to cope with the need for rapid, cost-effective and accurate identification of microorganisms. Several research teams have recently succeed in identifying moulds using MALDI-TOF MS, which was first adapted to bacteria, then to yeast identification. Since 2004, different commercial firms have released several ready-to-use MALDI-TOF MS platforms. This review describes the similarities and differences between the commercially available systems. In two parts, we first describe and compare the preprocessing and identification steps between the platforms and then compare the identification efficacy of yeast, moulds and dermatophytes species.
authors.affiliation: Parasitology and Mycology, Assistance Publique-Hôpitaux de Marseille, CHU Timone-Adultes, Marseilles CEDEX 5, France. carole.cassagne@ap-hm.fr.|Aix-Marseille University, UMR MD3 IP-TPT, Marseilles, France. carole.cassagne@ap-hm.fr.
authors.forename: Carole
authors.initials: C
authors.lastname: Cassagne
authors.affiliation: Parasitology and Mycology, Assistance Publique-Hôpitaux de Marseille, CHU Timone-Adultes, Marseilles CEDEX 5, France.
authors.forename: Anne-Cécile
authors.initials: AC
authors.lastname: Normand
authors.affiliation: Parasitology and Mycology, Assistance Publique-Hôpitaux de Marseille, CHU Timone-Adultes, Marseilles CEDEX 5, France.|Aix-Marseille University, UMR MD3 IP-TPT, Marseilles, France.
authors.forename: Coralie
authors.initials: C
authors.lastname: L'Ollivier
authors.affiliation: Parasitology and Mycology, Assistance Publique-Hôpitaux de Marseille, CHU Timone-Adultes, Marseilles CEDEX 5, France.|Aix-Marseille University, UMR MD3 IP-TPT, Marseilles, France.
authors.forename: Stéphane
authors.identifier: http://orcid.org/0000-0003-3293-5276
authors.initials: S
authors.lastname: Ranque
authors.affiliation: Parasitology and Mycology, Assistance Publique-Hôpitaux de Marseille, CHU Timone-Adultes, Marseilles CEDEX 5, France.|Aix-Marseille University, UMR MD3 IP-TPT, Marseilles, France.
authors.forename: Renaud
authors.initials: R
authors.lastname: Piarroux
mesh_terms: D003883:Arthrodermataceae / Q000145:classification / Q000302:isolation & purification; D005658:Fungi / Q000145:classification* / Q000302:isolation & purification*; D006801:Humans; D009181:Mycoses / Q000175:diagnosis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000191:economics / Q000295:instrumentation / Q000379:methods / Q000592:standards; D015003:Yeasts / Q000302:isolation & purification
keywords: MALDI-TOF mass spectrometry; clinical laboratory; dermatophyte; diagnostic; mould; yeast