title: Substrate specificity of FUT8 and chemoenzymatic synthesis of core-fucosylated asymmetric N-glycans.
abstract: Substrate specificity studies of human FUT8 using 77 structurally-defined N-glycans as acceptors showed a strict requirement towards the α1,3-mannose branch, but a great promiscuity towards the α1,6-mannose branch. Accordingly, a chemoenzymatic strategy was developed for the efficient synthesis of core-fucosylated asymmetric N-glycans.
authors.affiliation: Department of Chemistry and Center for Diagnostics & Therapeutics, Georgia State University, Atlanta, GA 30303, USA. lli22@gsu.edu pwang11@gsu.edu.
authors.forename: Angie D
authors.initials: AD
authors.lastname: Calderon
authors.affiliation: Department of Chemistry and Center for Diagnostics & Therapeutics, Georgia State University, Atlanta, GA 30303, USA. lli22@gsu.edu pwang11@gsu.edu and Chemily LLC, Atlanta, GA 30303, USA.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Chemistry and Center for Diagnostics & Therapeutics, Georgia State University, Atlanta, GA 30303, USA. lli22@gsu.edu pwang11@gsu.edu.
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Chemistry and Center for Diagnostics & Therapeutics, Georgia State University, Atlanta, GA 30303, USA. lli22@gsu.edu pwang11@gsu.edu.
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Chemistry and Center for Diagnostics & Therapeutics, Georgia State University, Atlanta, GA 30303, USA. lli22@gsu.edu pwang11@gsu.edu.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Chemistry and Center for Diagnostics & Therapeutics, Georgia State University, Atlanta, GA 30303, USA. lli22@gsu.edu pwang11@gsu.edu.
authors.forename: Peng G
authors.initials: PG
authors.lastname: Wang
mesh_terms: D055162:Biocatalysis; D005643:Fucose / Q000737:chemistry* / Q000378:metabolism; D005647:Fucosyltransferases / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D011134:Polysaccharides / Q000096:biosynthesis* / Q000737:chemistry; D013379:Substrate Specificity
chemical_list: D011134:Polysaccharides; D005643:Fucose; D005647:Fucosyltransferases; C020559:Glycoprotein 6-alpha-L-fucosyltransferase