title: Production, purification and structural study of an exopolysaccharide from Lactobacillus plantarum BC-25.
abstract: The exopolysaccharides (EPS) produced by Se-enriched Lactobacillus plantarum BC-25 was purified to illustrate its structure and conformational characterization. The yield of EPS (324.80mg/l) was obtained with a sodium selenite concentration of 6μg/ml. The results indicated that the EPS was soluble in water, but insoluble in organic solvents. The molecular weight of this highly thermal stability EPS was 1.83×10(4)Da and 1.33×10(4)Da with or without Se enriched respectively. The EPS was composed of mannose, galactose and glucose in a molar ratio of 92.21:1.79:6.00 and 91.36:2.44:6.20 with or without Se. This compound had a backbone of (1→2)-linked Man, (1→2.6)-linked Glc, (2→6)-linked Man, and (2→6)-linkedGal confirmed by GC-MS. IR analysis suggested that the EPS belonged to heteropolysaccharide with a pyran group, with possible presence of SeO and CSeC residues that Se may substitutes CH3 in -OCH3 in the polysaccharide as confirmed by NMR spectroscopy.
authors.affiliation: College of Food Science, Sichuan Agricultural University, Ya'an 625014, China. Electronic address: kang_zhou@163.com.
authors.forename: Kang
authors.initials: K
authors.lastname: Zhou
authors.affiliation: College of Food Science, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Yiting
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: College of Food Science, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Menglu
authors.initials: M
authors.lastname: Yang
authors.affiliation: College of Food Science, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Shujuan
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Food Science, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Li
authors.initials: L
authors.lastname: He
authors.affiliation: College of Food Science, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Ao
authors.affiliation: College of Resources, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Likou
authors.initials: L
authors.lastname: Zou
authors.affiliation: College of Food Science, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Shuliang
authors.initials: S
authors.lastname: Liu
mesh_terms: D002240:Carbohydrate Sequence; D048191:Lactiplantibacillus plantarum / Q000737:chemistry* / Q000378:metabolism; D009005:Monosaccharides / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification*; D012643:Selenium / Q000737:chemistry
chemical_list: D009005:Monosaccharides; D011135:Polysaccharides, Bacterial; D012643:Selenium
keywords: Exopolysaccharides; Lactobacillus plantarum; Selenium; Structure