title: Eucommia ulmoides extracts prevent the formation of advanced glycation end products.
abstract: Proteins non-enzymatically react with reducing sugars to form advanced glycation end-products (AGEs), resulting in the induction of protein denaturation. Because the levels of AGE increase with age and are elevated in age-related diseases, such as diabetes and atherosclerosis, the intake of compound(s) that inhibit the formation of AGEs by daily meal may represent a potential strategy for preventing age-related diseases. In this study, we measured the inhibitory effects of several Eucommia ulmoides extracts on the formation of AGEs, N(ε)-(carboxymethyl)lysine (CML) and N(ω)-(carboxymethyl)arginine (CMA). Although a crude extract obtained from E. ulmoides bark is widely used as herbal medicine, E. ulmoides leaf extract (ELE) inhibited CML and CMA formation more effectively during the incubation of gelatin with ribose. Therefore, the inhibitory effects of compounds present in ELE on CML and CMA formation were studied. As a result, isoquercetin showed the strongest inhibitory effect of all the tested ELE components. These results indicate that the oral intake of ELE may inhibit the formation of AGEs, thereby ameliorating age-related diseases.
authors.affiliation: Laboratory of Food and Regulation Biology, Department of Bioscience, School of Agriculture, Tokai University, Japan. nagai-883@umin.ac.jp.
authors.forename: Hikari
authors.initials: H
authors.lastname: Sugawa
authors.forename: Rei-Ichi
authors.initials: R
authors.lastname: Ohno
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Shirakawa
authors.forename: Akari
authors.initials: A
authors.lastname: Nakajima
authors.forename: Amane
authors.initials: A
authors.lastname: Kanagawa
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Hirata
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Ikeda
authors.forename: Narumi
authors.initials: N
authors.lastname: Moroishi
authors.forename: Mime
authors.initials: M
authors.lastname: Nagai
authors.forename: Ryoji
authors.initials: R
authors.lastname: Nagai
mesh_terms: D001120:Arginine / Q000031:analogs & derivatives / Q000737:chemistry; D031283:Eucommiaceae / Q000737:chemistry*; D005419:Flavonoids / Q000494:pharmacology; D005632:Fructose / Q000737:chemistry; D005690:Galactose / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D017127:Glycation End Products, Advanced / Q000737:chemistry*; D006027:Glycosides / Q000494:pharmacology; D008239:Lysine / Q000031:analogs & derivatives / Q000737:chemistry; D008358:Mannose / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D012266:Ribose / Q000737:chemistry; D053719:Tandem Mass Spectrometry
chemical_list: D005419:Flavonoids; D017127:Glycation End Products, Advanced; D006027:Glycosides; C408696:N(omega)-carboxymethylarginine; D010936:Plant Extracts; D005632:Fructose; D012266:Ribose; C048496:N(6)-carboxymethyllysine; D005780:Gelatin; D001120:Arginine; D005947:Glucose; D008239:Lysine; D008358:Mannose; D005690:Galactose