title: Prognosis of Castration-resistant Prostate Cancer Patients - Use of the AdnaTest® System for Detection of Circulating Tumor Cells.
abstract: BACKGROUND
  Detection of circulating tumor cells (CTC) in patients with castration-resistant prostate cancer (CRPC) may improve the estimate of chemotherapy response. We evaluated the AdnaTest® system in patients receiving docetaxel.
  
  
  METHODS
  CTC analysis was carried out in 37 patients by immunomagnetic separation. Correlation between serum prostate-specific antigen (sPSA) change and CTC presence and the influence of each parameter on the overall survival (OS) were evaluated.
  
  
  RESULTS
  We detected CTCs in 32 and 16 patients before and after three docetaxel cycles, respectively. The sPSA level correlated with CTC positivity during docetaxel therapy (p=0.0031). The longest OS was in patients negative for CTCs in both samples (p=0.0228). Change in sPSA levels was associated with treatment response (p=0.033).
  
  
  CONCLUSIONS
  We detected CTCs in a considerable number of patients with CRPC. The absolute change of sPSA level correlated with OS. CTC presence during docetaxel therapy was associated with shorter OS.
authors.affiliation: Department of Urology, General University Hospital and of The First Faculty of Medicine of Charles University otakar.capoun@seznam.cz.
authors.forename: Otakar
authors.initials: O
authors.lastname: Čapoun
authors.affiliation: Institute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital and of The First Faculty of Medicine of Charles University.
authors.forename: Veronika
authors.initials: V
authors.lastname: Mikulová
authors.affiliation: Institute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital and of The First Faculty of Medicine of Charles University.
authors.forename: Markéta
authors.initials: M
authors.lastname: Jančíková
authors.affiliation: Department of Oncology, General University Hospital and of The First Faculty of Medicine of Charles University.
authors.forename: Hana
authors.initials: H
authors.lastname: Honová
authors.affiliation: Department of Tumor Biology, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
authors.forename: Katarína
authors.initials: K
authors.lastname: Kološtová
authors.affiliation: Department of Urology, General University Hospital and of The First Faculty of Medicine of Charles University.
authors.forename: Roman
authors.initials: R
authors.lastname: Sobotka
authors.affiliation: Department of Urology, General University Hospital and of The First Faculty of Medicine of Charles University.
authors.forename: Pešl
authors.initials: P
authors.lastname: Michael
authors.affiliation: Institute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital and of The First Faculty of Medicine of Charles University.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Zima
authors.affiliation: Department of Urology, General University Hospital and of The First Faculty of Medicine of Charles University.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Hanuš
authors.affiliation: Department of Urology, General University Hospital and of The First Faculty of Medicine of Charles University.
authors.forename: Viktor
authors.initials: V
authors.lastname: Soukup
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000970:Antineoplastic Agents / Q000627:therapeutic use; D000077143:Docetaxel; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D009360:Neoplastic Cells, Circulating; D011379:Prognosis; D017430:Prostate-Specific Antigen / Q000097:blood; D064129:Prostatic Neoplasms, Castration-Resistant / Q000097:blood* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D043823:Taxoids / Q000627:therapeutic use; D016896:Treatment Outcome
chemical_list: D000970:Antineoplastic Agents; D043823:Taxoids; D000077143:Docetaxel; D017430:Prostate-Specific Antigen
keywords: AdnaTest; castration-resistant prostate cancer; chemotherapy; circulating tumor cells; prognosis