title: Bacterial biodegradation of melamine-contaminated aged soil: influence of different pre-culture media or addition of activation material.
abstract: This study aimed to investigate the biodegrading potential of Arthrobacter sp. MCO, Arthrobacter sp. CSP, and Nocardioides sp. ATD6 in melamine-contaminated upland soil (melamine: approx. 10.5 mg/kg dry weight) after 30 days of incubation. The soil sample used in this study had undergone annual treatment of lime nitrogen, which included melamine; it was aged for more than 10 years in field. When R2A broth was used as the pre-culture medium, Arthrobacter sp. MCO could degrade 55 % of melamine after 30 days of incubation, but the other strains could hardly degrade melamine (approximately 25 %). The addition of trimethylglycine (betaine) in soil as an activation material enhanced the degradation rate of melamine by each strain; more than 50 % of melamine was degraded by all strains after 30 days of incubation. In particular, strain MCO could degrade 72 % of melamine. When the strains were pre-cultured in R2A broth containing melamine, the degradation rate of melamine in soil increased remarkably. The highest (72 %) melamine degradation rate was noted when strain MCO was used with betaine addition.
authors.affiliation: Organochemicals Division, National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Tsukuba, Ibaraki, 305-8604, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Hatakeyama
authors.affiliation: Organochemicals Division, National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Tsukuba, Ibaraki, 305-8604, Japan. ktakagi@niaes.affrc.go.jp.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Takagi
mesh_terms: D000192:Actinomycetales / Q000378:metabolism*; D001173:Arthrobacter / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*; D014227:Triazines / Q000032:analysis / Q000378:metabolism*
chemical_list: D003470:Culture Media; D012989:Soil Pollutants; D014227:Triazines; C011907:melamine
keywords: Arthrobacter sp.; Betaine; Bioremediation; Biostimulation; Melamine; Nocardioides sp.