title: Gd (III) complex conjugate of low-molecular-weight chitosan as a contrast agent for magnetic resonance/fluorescence dual-modal imaging.
abstract: The fusion of molecular and anatomical modalities facilitates more reliable and accurate detection in clinic. In this work, we prepared gadolinium (III) complex Gd-DTPA-FITC-CS11 with magnetic resonance (MR) and fluorescence dual-modal imaging modalities. Gd-DTPA-FITC-CS11 consisted of fluorescein isothiocyanate and low-molecular-weight chitosan (CS11) units conjugated with gadolinium diethylenetriamine pentaacetic acid (Gd-DTPA). Gd-DTPA-FITC-CS11 exhibited a higher longitudinal relaxivity (14.09 mM(-1)s(-1)) than the clinical Gd-DTPA (3.85 mM(-1)s(-1)). T1-weighted MR contrast enhancement was also demonstrated the comparability to Gd-DTPA at lower dosage. The binding with bovine serum albumin (BSA) was investigated. The fluorescence of BSA in the presence of Gd-DTPA-FITC-CS11 was weakened due to static quenching mechanism. The conformation of BSA was slightly changed but α-helix was dominant. The binding was entropy-driven and spontaneous and the main contribution was hydrophobic interaction. Our results suggested the potential of Gd-DTPA-FITC-CS11 as an MR/fluorescence dual-modal imaging contrast agent in improving the diagnostic sensitivity and accuracy.
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China; Department of Ecological Agriculture, Bolgatanga Polytechnic, Bolgatanga, Ghana.
authors.forename: Peter Osei
authors.initials: PO
authors.lastname: Boamah
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Gong
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China; Hainan Provincial Key Lab of Fine Chemistry, Hainan University, Haikou 570228, China. Electronic address: qzhang@ujs.edu.cn.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Mingqing
authors.initials: M
authors.lastname: Hua
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Ye
mesh_terms: D000818:Animals; D002151:Calorimetry; D002417:Cattle; D048271:Chitosan / Q000138:chemical synthesis / Q000737:chemistry*; D002850:Chromatography, Gel; D002942:Circular Dichroism; D003287:Contrast Media / Q000138:chemical synthesis / Q000737:chemistry*; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry*; D005682:Gadolinium / Q000737:chemistry*; D008279:Magnetic Resonance Imaging; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003287:Contrast Media; D056831:Coordination Complexes; D012710:Serum Albumin, Bovine; D048271:Chitosan; D005682:Gadolinium
keywords: Contrast agents; Dual-modal imaging; Fluorescence; Gadolinium (III) complex; Low-molecular-weight chitosan; Magnetic resonance (MR) imaging