title: Facile method to prepare silk fibroin/hyaluronic acid films for vascular endothelial growth factor release.
abstract: A facile approach was proposed to prepare silk fibroin (SF) and hyaluronic acid (HA) composite films from aqueous solution without crosslinking or any post treatment. Only by controlling the HA content and film formation temperature during the film casting, the HA/SF films with different composition were prepared. The films were then characterized by structural characteristics, thermal stability, morphology, water stability, water absorption, mechanical properties. After immersing in water for 24h, all of the films showed good structural integrity. The degradation rate of the HA/SF films in protease XIV can be controlled by changing the film formation temperature and HA content. Decreasing the temperature and adding HA resulted in the rapid release of VEGF (vascular endothelial growth factor) from the HA/SF films. Overall, the 5% HA/SF films formed at 37°C with more rapid VEGF release exhibited great potential in drug delivery, especially when the rapid vascularization was needed.
authors.affiliation: National Engineering Research Center for Nanotechnology, Shanghai, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhou
authors.affiliation: School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Medical Imaging, Jinan Military General Hospital, Jinan, Shandong, People's Republic of China.
authors.forename: Xunwei
authors.initials: X
authors.lastname: Liu
authors.affiliation: School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Shi
authors.affiliation: National Engineering Research Center for Nanotechnology, Shanghai, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhu
authors.affiliation: National Engineering Research Center for Nanotechnology, Shanghai, People's Republic of China.
authors.forename: Daixu
authors.initials: D
authors.lastname: Wei
authors.affiliation: National Engineering Research Center for Nanotechnology, Shanghai, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Department of Medical Imaging, Jinan Military General Hospital, Jinan, Shandong, People's Republic of China. Electronic address: cjr.sungang@vip.163.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Sun
authors.affiliation: School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, People's Republic of China; National Engineering Research Center for Nanotechnology, Shanghai, People's Republic of China. Electronic address: hdn_nercn@163.com.
authors.forename: Dannong
authors.initials: D
authors.lastname: He
mesh_terms: D000818:Animals; D012831:Bombyx; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D005349:Fibroins / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D016062:Porosity; D011402:Pronase / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013696:Temperature; D013718:Tensile Strength; D042461:Vascular Endothelial Growth Factor A / Q000737:chemistry*; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D004337:Drug Carriers; D042461:Vascular Endothelial Growth Factor A; C482288:fibroin, silkworm; D014867:Water; D006820:Hyaluronic Acid; D005349:Fibroins; D011402:Pronase
keywords: Facile method; Film; Hyaluronic acid; Silk fibroin; VEGF release