title: Development of Cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene.
abstract: Cotton leaf curl virus (CLCuV) is a serious pathogen causing leaf curl disease and affecting the cotton production in major growing areas. The transgenic cotton (Gossypium hirsutum cv. Coker 310) plants were developed by using βC1 gene in antisense orientation gene driven by Cauliflower mosaic virus-35S promoter and nos (nopaline synthase) terminator and mediated by Agrobacterium tumefaciens transformation and somatic embryogenesis system. Molecular confirmation of the transformants was carried out by polymerase chain reaction (PCR) and Southern blot hybridization. The developed transgenic and inoculated plants remained symptomless till their growth period. In conclusion, the plants were observed as resistant to CLCuV.
authors.affiliation: King Fahd Medical Research Center, King Abdulaziz University, Post Box No. 80216, Jeddah 21589, Saudi Arabia.
authors.forename: Sayed Sartaj
authors.initials: SS
authors.lastname: Sohrab
authors.affiliation: King Fahd Medical Research Center, King Abdulaziz University, Post Box No. 80216, Jeddah 21589, Saudi Arabia.
authors.forename: Mohammad A
authors.initials: MA
authors.lastname: Kamal
authors.affiliation: Faculty of Medical Technology, Omar Al Mukhtar University, Tobruk, Libya.
authors.forename: Abdul
authors.initials: A
authors.lastname: Ilah
authors.affiliation: Department of Biology, College of Natural and Computational Sciences, University of Gondar, Post Box No. 196, Gondar, Ethiopia.
authors.forename: Azamal
authors.initials: A
authors.lastname: Husen
authors.affiliation: Division of Biotechnology, JK-AgriGenetics Ltd., Hyderabad, A.P., India.
authors.forename: P S
authors.initials: PS
authors.lastname: Bhattacharya
authors.affiliation: Division of Biotechnology, JK-AgriGenetics Ltd., Hyderabad, A.P., India.
authors.forename: D
authors.initials: D
authors.lastname: Rana
keywords: A. tumefaciens, Agrobacterium tumefaciens; CLCuV resistance; CLCuV, Cotton leaf curl virus; CP, coat protein; Gene transfer; MS, Murashige and Skoog’s; Molecular verification; PCR, polymerase chain reaction; PDR, pathogen derived resistance; Transgenic cotton plants; Virus resistance