title: TDRP deficiency contributes to low sperm motility and is a potential risk factor for male infertility.
abstract: TDRP (Testis Development-Related Protein), a nuclear factor, might play an important role in spermatogenesis. However, the molecular mechanisms of TDRP underlying these fundamental processes remain elusive. In this study, a Tdrp-deficient mouse model was generated. Fertility tests and semen analysis were performed. Tdrp-deficient mice were not significantly different from wild-type littermates in development of testes, genitourinary tract, or sperm count. Morphologically, spermatozoa of the Tdrp-deficient mice was not significantly different from the wild type. Several sperm motility indexes, i.e. the average path velocity (VAP), the straight line velocity (VSL) and the curvilinear velocity (VCL) were significantly decreased in Tdrp-deficient mice (p<0.05). The proportion of slow velocity sperm also increased significantly in the mutant mice (p<0.05). However, fertility tests showed that no significant difference inaverage offspring amount (AOA), frequency of copulatory plug (FCP), and frequency of conception (FC). Furthermore, TDRP1 could interact with PRM2, which might be the molecular mechanism of its nuclear function in spermatozoa. In conclusion, these data collectively demonstrated that Tdrp deficiency impaired the sperm motility, but Tdrp deficiency alone was not sufficient to cause male infertility in mice. Additionally, TDRP1 might participate in spermatogenes is through interaction with PRM2.
authors.affiliation: Department of Urology, Huashan Hospital, Fudan University 12 Central Urumqi Rd, Shanghai 200040, P.R. China.
authors.forename: Shanhua
authors.initials: S
authors.lastname: Mao
authors.affiliation: Department of Urology, Huashan Hospital, Fudan University 12 Central Urumqi Rd, Shanghai 200040, P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wu
authors.affiliation: Department of Endocrinology, Huashan Hospital, Fudan University 12 Central Urumqi Rd, Shanghai 200040, P.R. China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Cao
authors.affiliation: Department of Endocrinology, Huashan Hospital, Fudan University 12 Central Urumqi Rd, Shanghai 200040, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: He
authors.affiliation: Department of Endocrinology, Huashan Hospital, Fudan University 12 Central Urumqi Rd, Shanghai 200040, P.R. China.
authors.forename: Naijia
authors.initials: N
authors.lastname: Liu
authors.affiliation: Department of Endocrinology, Huashan Hospital, Fudan University 12 Central Urumqi Rd, Shanghai 200040, P.R. China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Endocrinology, Huashan Hospital, Fudan University 12 Central Urumqi Rd, Shanghai 200040, P.R. China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Urology, Huashan Hospital, Fudan University 12 Central Urumqi Rd, Shanghai 200040, P.R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ding
authors.affiliation: Department of Endocrinology, Huashan Hospital, Fudan University 12 Central Urumqi Rd, Shanghai 200040, P.R. China.
authors.forename: Xuanchun
authors.initials: X
authors.lastname: Wang
keywords: PRM2; TDRP (Tdrp1 ); gene knockout mouse model; sperm