title: Distribution of olfactory marker protein in the rat vomeronasal organ.
abstract: Olfactory marker protein (OMP) may act as a modulator within the olfactory signal-transduction cascade. It has also been shown to have some importance in development of olfactory sensory organs. Here we used high resolution immunocytochemistry to localize OMP in the rat vomeronasal organ (VNO). Immunofluorescence for OMP was abundant in cilia and in apical dendrites of sensory cells, mostly associated with intraepithelial capillaries. Perikarya were stained to a lesser extent while intense OMP immunoreactivity was seen in axons of sensory neurons. Single cells within the non-sensory portion of the VNO exhibited intense OMP immunofluorescence in apical cilia and weak cytoplasmic staining. Some of the exocrine cells in the vomeronasal glands contained OMP positive secretory granules. Electron microscopy revealed that non-sensory ciliated cells had short rod like kinocilia as well as microvilli. These cells contained secretory vesicles. Their basal portion was in close apposition to nerve endings. Our findings suggest that the sensory part of the VNO contains OMP positive sensory neurons and that the non-sensory epithelium may contain secondary sensory cells. In addition OMP may be liberated from secretory glands into vomeronasal secretions.
authors.affiliation: Institute of Anatomy II, University Hospital Jena, Friedrich Schiller University, Jena, Germany. Electronic address: Andrea.Rodewald@med.uni-jena.de.
authors.forename: A
authors.initials: A
authors.lastname: Rodewald
authors.affiliation: Institute of Anatomy II, University Hospital Jena, Friedrich Schiller University, Jena, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Gisder
authors.affiliation: Institute of Anatomy II, University Hospital Jena, Friedrich Schiller University, Jena, Germany.
authors.forename: V M
authors.initials: VM
authors.lastname: Gebhart
authors.affiliation: Institute of Anatomy II, University Hospital Jena, Friedrich Schiller University, Jena, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Oehring
authors.affiliation: Institute of Anatomy II, University Hospital Jena, Friedrich Schiller University, Jena, Germany.
authors.forename: G F
authors.initials: GF
authors.lastname: Jirikowski
mesh_terms: D000818:Animals; D002196:Capillaries / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D002923:Cilia / Q000378:metabolism / Q000648:ultrastructure; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D051842:Olfactory Marker Protein / Q000096:biosynthesis* / Q000235:genetics; D009831:Olfactory Mucosa / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D011984:Sensory Receptor Cells / Q000378:metabolism / Q000648:ultrastructure; D019147:Vomeronasal Organ / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D051842:Olfactory Marker Protein; C497599:Omp protein, rat
keywords: Electron microscopy; Immunohistochemistry; Non-sensory epithelium; Olfactory marker protein; Sensory neurons; Vomeronasal glands; Vomeronasal organ