title: Internalized compartments encapsulated nanogels for targeted drug delivery.
abstract: Drug delivery systems inspired by natural particulates hold great promise for targeted cancer therapy. An endosome formed by internalization of plasma membrane has a massive amount of membrane proteins and receptors on the surface, which is able to specifically target the homotypic cells. Herein, we describe a simple method to fabricate an internalized compartments encapsulated nanogel with endosome membrane components (EM-NG) from source cancer cells. Following intracellular uptake of methacrylated hyaluronic acid (m-HA) adsorbed SiO2/Fe3O4 nanoparticles encapsulating a crosslinker and a photoinitiator, EM-NG was readily prepared through in situ crosslinking initiated under UV irradiation after internalization. The resulting nanogels loaded with doxorubicin (DOX) displayed enhanced internalization efficiency to the source cells through a specific homotypic affinity in vitro. However, when treated with the non-source cells, the EM-NGs exhibited insignificant difference in therapeutic efficiency compared to a bare HA nanogel with DOX. This study illustrates the potential of utilizing an internalized compartments encapsulated formulation for targeted cancer therapy, and offers guidelines for developing a natural particulate-inspired drug delivery system.
authors.affiliation: Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Raleigh, NC 27695, USA. zgu@email.unc.edu and Molecular Pharmaceutics Division, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
authors.forename: Jicheng
authors.initials: J
authors.lastname: Yu
authors.affiliation: Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Raleigh, NC 27695, USA. zgu@email.unc.edu and Molecular Pharmaceutics Division, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Raleigh, NC 27695, USA. zgu@email.unc.edu and Molecular Pharmaceutics Division, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
authors.forename: Wujin
authors.initials: W
authors.lastname: Sun
authors.affiliation: Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Raleigh, NC 27695, USA. zgu@email.unc.edu and Molecular Pharmaceutics Division, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Raleigh, NC 27695, USA. zgu@email.unc.edu.
authors.forename: Davis
authors.initials: D
authors.lastname: Ranson
authors.affiliation: Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Raleigh, NC 27695, USA. zgu@email.unc.edu and Molecular Pharmaceutics Division, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
authors.forename: Yanqi
authors.initials: Y
authors.lastname: Ye
authors.affiliation: Center for Soft Condensed Matter Physics and Interdisciplinary Research & College of Physics, Optoelectronics and Energy, Soochow University, Suzhou, 215006, China. wengyy@suda.edu.cn.
authors.forename: Yuyan
authors.initials: Y
authors.lastname: Weng
authors.affiliation: Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Raleigh, NC 27695, USA. zgu@email.unc.edu and Molecular Pharmaceutics Division, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA and Department of Medicine, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Gu
mesh_terms: D004317:Doxorubicin / Q000008:administration & dosage; D016503:Drug Delivery Systems; D005296:Ferrous Compounds; D006820:Hyaluronic Acid; D053758:Nanoparticles; D012822:Silicon Dioxide
chemical_list: D005296:Ferrous Compounds; D012822:Silicon Dioxide; D004317:Doxorubicin; D006820:Hyaluronic Acid; C034236:ferrous oxide