title: Ethanolamine enhances the proliferation of intestinal epithelial cells via the mTOR signaling pathway and mitochondrial function.
abstract: Ethanolamine (Etn), which is the base constituent of phosphatidylethanolamine, a major phospholipid in animal cell membranes, is required for the proliferation of many types of mammalian epithelial cells. However, it is not clear whether the proliferation of intestinal epithelial cells requires Etn. The present study was conducted to examine the effects of Etn on the proliferation of intestinal epithelial cells and to elucidate the underlying mechanisms. The addition of Etn at 100 or 200 μM was found to enhance the proliferation of IPEC-1 cells. The expression of cell cycle-related proteins CDK4, RB3, cyclin A, and PCNA was also enhanced by Etn. Moreover, the expression or phosphorylation levels of the mammalian target of rapamycin (mTOR) signaling pathway protein and the expression of proteins related to mitochondrial function were also affected by Etn in IPEC-1 cells. These results indicate that Etn promotes the proliferation of intestinal epithelial cells by exerting effects on mTOR signaling pathway and mitochondrial function.
authors.affiliation: Observation and Experiment Station of Animal Nutrition and Feed Science in South-Central China, Ministry of Agriculture; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Yuandaer Road 644, Changsha, Hunan Province, 410125, People's Republic of China.|School of Life Sciences, Hunan Normal University, Changsha, Hunan, People's Republic of China.|Fujian Aonong Biotechnology Corporation, Xiamen, Fujian, People's Republic of China.
authors.forename: Huansheng
authors.initials: H
authors.lastname: Yang
authors.affiliation: Observation and Experiment Station of Animal Nutrition and Feed Science in South-Central China, Ministry of Agriculture; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Yuandaer Road 644, Changsha, Hunan Province, 410125, People's Republic of China.|National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients from Botanicals, Provincial Co-Innovation Center for Utilization of Botanical Function Ingredients, Hunan Agricultural University, Changsha, Hunan, 410128, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Xiong
authors.affiliation: Observation and Experiment Station of Animal Nutrition and Feed Science in South-Central China, Ministry of Agriculture; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Yuandaer Road 644, Changsha, Hunan Province, 410125, People's Republic of China.
authors.forename: Tiejun
authors.initials: T
authors.lastname: Li
authors.affiliation: Observation and Experiment Station of Animal Nutrition and Feed Science in South-Central China, Ministry of Agriculture; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Yuandaer Road 644, Changsha, Hunan Province, 410125, People's Republic of China. isayulong_yin@sina.com.|School of Life Sciences, Hunan Normal University, Changsha, Hunan, People's Republic of China. isayulong_yin@sina.com.|National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients from Botanicals, Provincial Co-Innovation Center for Utilization of Botanical Function Ingredients, Hunan Agricultural University, Changsha, Hunan, 410128, China. isayulong_yin@sina.com.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Yin
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D019856:Ethanolamine / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D007413:Intestinal Mucosa / Q000166:cytology*; D008928:Mitochondria / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects; D013552:Swine; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D019856:Ethanolamine; D058570:TOR Serine-Threonine Kinases
keywords: Epithelial cells; Ethanolamine; Intestine; Proliferation; mTOR signaling pathway