title: The complete mitochondrial genome of the Atylotus miser (Diptera: Tabanomorpha: Tabanidae), with mitochondrial genome phylogeny of lower Brachycera (Orthorrhapha).
abstract: Brachycera is a clade with over 80,000 described species and originated from the Mesozoic, and its larvae employ comprehensive feeding strategies. The phylogeny of the lower Brachycera has been studied intensively over the past decades. In order to supplement the lack of genetic data in this important group, we sequenced the complete mitochondrial (mt) genome of Atylotus miser as well as the nearly complete mt genomes of another 11 orthorrhaphous flies. The mt genome of A. miser is 15,858bp, which is typical of Diptera, with 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and a 993bp control region. The rest of the orthorrhaphous mt genomes in our study have the similar structure with A. miser. Additionally, we conducted a phylogenetic analysis of 20 mt genomes using Maximum-likelihood and Bayesian methods in order to reconstruct the evolutionary relationship of Orthorrhapha. The results show that all infraorders of Brachycera are monophyletic, and a relationship of Tabanomorpha+((Xylophagomorpha+Stratiomyomorpha)+Muscomorpha) has been proposed. Within Xylophagomorpha, Nemestrinoidae forms the sister group of Xylophagidae.
authors.affiliation: Department of Entomology, China Agricultural University, Beijing, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Entomology, China Agricultural University, Beijing, China.
authors.forename: Xuankun
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Entomology, China Agricultural University, Beijing, China.
authors.forename: Shuangmei
authors.initials: S
authors.lastname: Ding
authors.affiliation: Department of Entomology, China Agricultural University, Beijing, China; Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.affiliation: School of Biological Sciences, University of Wollongong, Wollongong, Australia.
authors.forename: Meng
authors.initials: M
authors.lastname: Mao
authors.affiliation: Department of Entomology, China Agricultural University, Beijing, China; Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China. Electronic address: mqwang@ippcaas.cn.
authors.forename: Mengqing
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Entomology, China Agricultural University, Beijing, China. Electronic address: dyangcau@126.com.
authors.forename: Ding
authors.initials: D
authors.lastname: Yang
mesh_terms: D000818:Animals; D004175:Diptera / Q000235:genetics*; D054629:Genome, Mitochondrial; D019476:Insect Proteins / Q000235:genetics; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics
chemical_list: D019476:Insect Proteins; D012335:RNA, Ribosomal; D012343:RNA, Transfer
keywords: Brachycera; Diptera; Mitochondrial genome; Phylogeny