title: An Alternative Approach to Synthesizing Galactooligosaccharides by Cell-Surface Display of β-Galactosidase on Yarrowia lipolytica.
abstract: An alternative strategy for synthesizing galactooligosaccharides (GOS) from an erythritol-producing yeast Yarrowia lipolytica using surface display technology was demonstrated. The engineered strain CGMCC11369 was developed by fusion of the β-galactosidase gene from Aspergillus oryzae to the YlPir1 gene, which codes for a cell wall protein. β-Galactosidase was effectively displayed on the cell surface of Yarrowia lipolytica start strain CGMCC7326. This engineered strain with surface-displayed β-galactosidase efficiently synthesized GOS from lactose. An amount of 160 g/L GOS was produced within 6 h in a solution of 500 g/L lactose and 5 mg/mL cell (dry weight) at pH 5.5 and 60 °C, with a yield of 51% of consumed lactose monohydrate. This newly developed method was applied with waste yeast paste from erythritol industry at least 10 times. The optimal reaction temperature increased to 60 °C, about 20 °C higher than that of free β-galactosidase, which was helpful for enhancing the reaction rate and GOS production.
authors.affiliation: State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University , Shanghai 200240, China.
authors.forename: Jin
authors.initials: J
authors.lastname: An
authors.affiliation: State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University , Shanghai 200240, China.
authors.forename: Lebin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University , Shanghai 200240, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Li
authors.affiliation: State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University , Shanghai 200240, China.
authors.forename: Dawen
authors.initials: D
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University , Shanghai 200240, China.
authors.forename: Huiling
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Innovation & Application Institute, Zhejiang Ocean University , Zhoushan 316022, China.
authors.forename: Hengwei
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University , Shanghai 200240, China.
authors.forename: Muhammad Zohaib
authors.initials: MZ
authors.lastname: Nawaz
authors.affiliation: State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University , Shanghai 200240, China.
authors.forename: Hairong
authors.initials: H
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University , Shanghai 200240, China.
authors.forename: Zixin
authors.initials: Z
authors.lastname: Deng
mesh_terms: D005690:Galactose / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D009844:Oligosaccharides / Q000378:metabolism*; D025062:Yarrowia / Q000201:enzymology / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D009844:Oligosaccharides; D001616:beta-Galactosidase; D005690:Galactose
keywords: Yarrowia lipolytica; cell surface display; erythritol; galactooligosaccharides; β-galactosidase