title: Ibuprofen Ingestion Does Not Affect Markers of Post-exercise Muscle Inflammation.
abstract: OBJECTIVE
  We investigated if oral ingestion of ibuprofen influenced leucocyte recruitment and infiltration following an acute bout of traditional resistance exercise Methods: Sixteen male subjects were divided into two groups that received the maximum over-the-counter dose of ibuprofen (1200mg d(-1)) or a similarly administered placebo following lower body resistance exercise. Muscle biopsies were taken from m.vastus lateralis and blood serum samples were obtained before and immediately after exercise, and at 3 and 24 h after exercise. Muscle cross-sections were stained with antibodies against neutrophils (CD66b and MPO) and macrophages (CD68). Muscle damage was assessed via creatine kinase and myoglobin in blood serum samples, and muscle soreness was rated on a ten-point pain scale.
  
  
  RESULTS
  The resistance exercise protocol stimulated a significant increase in the number of CD66b(+) and MPO(+) cells when measured 3 h post exercise. Serum creatine kinase, myoglobin and subjective muscle soreness all increased post-exercise. Muscle leucocyte infiltration, creatine kinase, myoglobin and subjective muscle soreness were unaffected by ibuprofen treatment when compared to placebo. There was also no association between increases in inflammatory leucocytes and any other marker of cellular muscle damage.
  
  
  CONCLUSIONS
  Ibuprofen administration had no effect on the accumulation of neutrophils, markers of muscle damage or muscle soreness during the first 24 h of post-exercise muscle recovery.
authors.affiliation: Centre for Physical Activity and Nutrition Research, School of Exercise and Nutrition Science, Deakin University Burwood, VIC, Australia.
authors.forename: Luke
authors.initials: L
authors.lastname: Vella
authors.affiliation: Liggins Institute, University of Auckland Auckland, New Zealand.
authors.forename: James F
authors.initials: JF
authors.lastname: Markworth
authors.affiliation: Department of Physical Performance, Norwegian School of Sport Science Oslo, Norway.
authors.forename: Gøran
authors.initials: G
authors.lastname: Paulsen
authors.affiliation: Department of Physical Performance, Norwegian School of Sport Science Oslo, Norway.
authors.forename: Truls
authors.initials: T
authors.lastname: Raastad
authors.affiliation: School of Biomedical Sciences and Institute of Health and Biomedical Innovation, Queensland University of Technology Brisbane, QLD, Australia.
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Peake
authors.affiliation: Centre for Physical Activity and Nutrition Research, School of Exercise and Nutrition Science, Deakin University Burwood, VIC, Australia.
authors.forename: Rod J
authors.initials: RJ
authors.lastname: Snow
authors.affiliation: Liggins Institute, University of Auckland Auckland, New Zealand.
authors.forename: David
authors.initials: D
authors.lastname: Cameron-Smith
authors.affiliation: Centre for Physical Activity and Nutrition Research, School of Exercise and Nutrition Science, Deakin University Burwood, VIC, Australia.
authors.forename: Aaron P
authors.initials: AP
authors.lastname: Russell
keywords: NSAID treatment; exercise recovery; inflammation; leucocyte; resistance exercise