title: Use of a modified hair strand test to assess the antifungal activity kinetics of dog hair after a 2% climbazole shampoo application.
abstract: BACKGROUND
  The "hair strand test" was first developed as a model to evaluate the antifungal activity of antidandruff shampoos.
  
  
  OBJECTIVE
  To assess the residual activity of an antifungal shampoo on the hair shafts of dogs after a single application, followed by bathing with a physiological shampoo one month later.
  
  
  METHODS
  Six beagles (two males and four females) from a research colony.
  
  
  METHODS
  Dogs were bathed with a 2% climbazole shampoo. Hairs were collected before application of the shampoo and at scheduled intervals for 30 days after treatment. A physiological shampoo was then applied to all dogs and hairs were collected following the same schedule. The inhibition zone around the hair shafts was measured after incubation on Sabouraud's dextrose agar plates streaked with three Malassezia pachydermatis strains.
  
  
  RESULTS
  Inhibition zones around hairs collected from dogs bathed with 2% climbazole shampoo were significantly larger than those around hairs collected before shampooing at all time points (P = 0.003). An increase in the width of the inhibition zones around climbazole treated hairs was observed following physiological shampoo on Day 30 (P = 0.005). No significant differences were observed between Malassezia pachydermatis isolates (P = 0.571). No inhibition zones were seen around the hairs of dogs bathed with physiological shampoo only.
  
  
  CONCLUSIONS
  The modified hair strand test is useful for the assessment of residual antifungal activity on animal hairs. Use of a physiological shampoo following antifungal shampoo therapy may increase the efficacy of the antifungal product for the control of Malassezia overgrowth.
authors.affiliation: Department of Parasitology, Mycology and Dermatology, Dynamyc Research Group, Université Paris-Est, Ecole nationale vétérinaire d'Alfort.|Institut de Recherche Clinique d'Alfort, École nationale vétérinaire d'Alfort, UPE, 94704, Maisons-Alfort, France.
authors.forename: Jean Yanique
authors.initials: JY
authors.lastname: Petit
authors.affiliation: Department of Parasitology, Mycology and Dermatology, Dynamyc Research Group, Université Paris-Est, Ecole nationale vétérinaire d'Alfort.
authors.forename: Paola
authors.initials: P
authors.lastname: Cavana
authors.affiliation: INRA, UMR1198 Biologie du Développement et Reproduction, École nationale vétérinaire d'Alfort, UPE, 94704, Maisons-Alfort, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Thoumire
authors.affiliation: Department of Parasitology, Mycology and Dermatology, Dynamyc Research Group, Université Paris-Est, Ecole nationale vétérinaire d'Alfort.|BioPôle d'Alfort, École nationale vétérinaire d'Alfort, UPE, 94704, Maisons-Alfort, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Guillot
authors.affiliation: Institut de Recherche Clinique d'Alfort, École nationale vétérinaire d'Alfort, UPE, 94704, Maisons-Alfort, France.|BioPôle d'Alfort, École nationale vétérinaire d'Alfort, UPE, 94704, Maisons-Alfort, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Perrot
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D004285:Dogs; D005260:Female; D006197:Hair / Q000737:chemistry*; D006203:Hair Preparations / Q000737:chemistry; D007093:Imidazoles / Q000737:chemistry / Q000494:pharmacology*; D008290:Malassezia / Q000187:drug effects; D008297:Male
chemical_list: D000935:Antifungal Agents; D006203:Hair Preparations; D007093:Imidazoles; C108286:climbazole