title: Biocompatible Fluorescent Core-Shell Nanoconjugates Based on Chitosan/Bi2S3 Quantum Dots.
abstract: Bismuth sulfide (Bi2S3) is a narrow-bandgap semiconductor that is an interesting candidate for fluorescent biomarkers, thermoelectrics, photocatalysts, and photovoltaics. This study reports the synthesis and characterization of novel Bi2S3 quantum dots (QDs) functionalized using chitosan (CHI) as the capping ligands via aqueous "green" route at room temperature and ambient pressure. Transmission electron microscopy (TEM), UV-visible (UV-vis) spectroscopy, photoluminescence (PL) spectroscopy, dynamic light scattering (DLS), and zeta potential (ZP) analysis were used to characterize the hybrids made of biopolymer-functionalized Bi2S3 semiconductor nanocrystals. The results demonstrated that the CHI ligand was effective at nucleating and controlling the growth of water-soluble colloidal Bi2S3 nanoparticles. The average sizes of the Bi2S3 nanoparticles were significantly affected by the molar ratio of the precursors but less dependent on the pH of the aqueous media, leading to the formation of nanocrystals with average diameters varying from 4.2 to 6.7 nm. These surface-modified Bi2S3 nanocrystals with CHI exhibited photoluminescence in the visible spectral region. Moreover, the results of in vitro MTT (3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide) assay with human osteosarcoma cells (SAOS) cell line demonstrated no cytotoxic response of the nanoconjugates.Furthermore, the results indicated that the Bi2S3 QD-CHI nanoconjugates showed HEK293T cell uptake; therefore, they can be potentially used as novel fluorescent nanoprobes for the in vitro bioimaging of cells in biomedical applications. Graphical Abstract Schematic representation of the biocompatible core-shell nanostructure of the chitosan/Bi2S3 quantum dot conjugates with photoluminescent properties.
authors.affiliation: Center of Nanoscience, Nanotechnology and Innovation - CeNano²I, Department of Metallurgical and Materials Engineering, Federal University of Minas Gerais, Av. Antônio Carlos, 6627-Escola de Engenharia, Bloco 2-Sala 2233, Belo Horizonte, Minas Gerais, 31.270-901, Brazil.
authors.forename: Fábio P
authors.initials: FP
authors.lastname: Ramanery
authors.affiliation: Center of Nanoscience, Nanotechnology and Innovation - CeNano²I, Department of Metallurgical and Materials Engineering, Federal University of Minas Gerais, Av. Antônio Carlos, 6627-Escola de Engenharia, Bloco 2-Sala 2233, Belo Horizonte, Minas Gerais, 31.270-901, Brazil.
authors.forename: Alexandra A P
authors.initials: AA
authors.lastname: Mansur
authors.affiliation: Center of Nanoscience, Nanotechnology and Innovation - CeNano²I, Department of Metallurgical and Materials Engineering, Federal University of Minas Gerais, Av. Antônio Carlos, 6627-Escola de Engenharia, Bloco 2-Sala 2233, Belo Horizonte, Minas Gerais, 31.270-901, Brazil. hmansur@demet.ufmg.br.
authors.forename: Herman S
authors.initials: HS
authors.lastname: Mansur
authors.affiliation: Center of Nanoscience, Nanotechnology and Innovation - CeNano²I, Department of Metallurgical and Materials Engineering, Federal University of Minas Gerais, Av. Antônio Carlos, 6627-Escola de Engenharia, Bloco 2-Sala 2233, Belo Horizonte, Minas Gerais, 31.270-901, Brazil.|Department of Preventive Veterinary Medicine, UFMG, Belo Horizonte, Brazil.
authors.forename: Sandhra M
authors.initials: SM
authors.lastname: Carvalho
authors.affiliation: Department of Physiology and Biophysics, UFMG, Belo Horizonte, Brazil.
authors.forename: Matheus C
authors.initials: MC
authors.lastname: Fonseca
keywords: Chitosan; Core-shell nanostructure; Nanoconjugates; Nanomaterial; Nanoparticles