title: Over-expression of Sox4 and β-catenin is associated with a less favorable prognosis of osteosarcoma.
abstract: The purpose of this study was to examine the association of the expression of Sox4 and β-catenin with the prognosis of osteosarcoma. A total of 108 cases of conventional osteosarcoma were involved in this study and 28 cases of osteochondroma served as controls. The expression of Sox4 and β-catenin was detected by using immunohistochemical staining and Western blotting. The results showed that Sox4 and β-catenin were over-expressed in 67 (62.03%) and 62 (57.41%) of 108 osteosarcoma cases, while in only 3 (10.71%) and 5 (17.86%) of 28 controls, respectively (P<0.05 for all). The expression of Sox4 and β-catenin was associated with the distant metastasis, pathological grade and Enneking stage of patients with osteosarcoma (P<0.05 for all). The mean overall survival time and the 5-year-survival rate in osteosarcoma patients with Sox4 and β-catenin over-expressed were significantly reduced as compared with those in Sox4 and β-catenin low-expression group (P<0.05 for all). Cox multifactor regression analysis revealed that the distant metastasis, Enneking stage, and the expression of Sox4 and β-catenin were independent risk factors of patients with osteosarcoma (P<0.05 for all). The findings indicated that overexpression of Sox4 and β-catenin is associated with a poor prognosis of osteosarcoma.
authors.affiliation: Department of Orthopaedics, the First Affiliated Hospital of Bengbu Medical College, Bengbu, 233000, China.
authors.forename: Zheng-Qi
authors.initials: ZQ
authors.lastname: Bao
authors.affiliation: Department of Orthopaedics, the First Affiliated Hospital of Bengbu Medical College, Bengbu, 233000, China.
authors.forename: Chang-Chun
authors.initials: CC
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedics, the First Affiliated Hospital of Bengbu Medical College, Bengbu, 233000, China.
authors.forename: Yu-Zhou
authors.initials: YZ
authors.lastname: Xiao
authors.affiliation: Department of Orthopaedics, the First Affiliated Hospital of Bengbu Medical College, Bengbu, 233000, China.
authors.forename: Jian-Sheng
authors.initials: JS
authors.lastname: Zhou
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, 233000, China.
authors.forename: Yi-Sheng
authors.initials: YS
authors.lastname: Tao
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, 233000, China. msautumn@163.com.
authors.forename: Da-Min
authors.initials: DM
authors.lastname: Chai
mesh_terms: D000293:Adolescent; D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D001859:Bone Neoplasms / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D002648:Child; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000556:secondary; D008297:Male; D008875:Middle Aged; D012516:Osteosarcoma / Q000378:metabolism* / Q000473:pathology; D055751:SOXC Transcription Factors / Q000235:genetics / Q000378:metabolism*; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; C083431:SOX4 protein, human; D055751:SOXC Transcription Factors; D051176:beta Catenin
keywords: Sox4; osteochondroma; osteosarcoma; prognosis; β-catenin