title: Membrane associated protein flotillin-2 in Litopenaeus vannamei plays a role in WSSV infection.
abstract: Flotillin-2, an important protein of vesicular endocytosis, plays an essential role in a large number of cellular processes, including viruses and pathogen infection. In the present study, a flotillin-2 homolog in Litopenaeus vannamei, designed as Lvflotillin-2, was cloned and characterized. To analyze the putative role of Lvflotillin-2 during white spot syndrome virus (WSSV) infection, real-time quantitative PCR was performed. The result showed that the transcriptional level of Lvflotillin-2 was up-regulated significantly after virus challenge. Furthermore, upon WSSV stimulation, Lvflotillin-2 in shrimp cells could translocate from the plasma membrane to intracellular compartments, and unexpectedly, also into nucleus. Additionally, depletion of Lvflotillin-2 inhibited WSSV gene ie1 transcription. It suggested that Lvflotillin-2 could be hijacked by WSSV. These observations indicated that Lvflotillin-2 was involved in WSSV infection, and presented here should be useful for gaining insight into shrimp immunity and WSSV pathogenesis.
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources of State Oceanic Administration, Third Institute of Oceanography, State Oceanic Administration, Key Laboratory of Marine Genetic Resources of Fujian Province, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen, 361005, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Shi
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources of State Oceanic Administration, Third Institute of Oceanography, State Oceanic Administration, Key Laboratory of Marine Genetic Resources of Fujian Province, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen, 361005, PR China; School of Life Science, Xiamen University, Xiamen, 361005, PR China.
authors.forename: Guangran
authors.initials: G
authors.lastname: Guo
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources of State Oceanic Administration, Third Institute of Oceanography, State Oceanic Administration, Key Laboratory of Marine Genetic Resources of Fujian Province, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen, 361005, PR China; School of Life Science, Xiamen University, Xiamen, 361005, PR China.
authors.forename: Rongdiao
authors.initials: R
authors.lastname: Liu
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources of State Oceanic Administration, Third Institute of Oceanography, State Oceanic Administration, Key Laboratory of Marine Genetic Resources of Fujian Province, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen, 361005, PR China; School of Life Science, Xiamen University, Xiamen, 361005, PR China.
authors.forename: Chuanqi
authors.initials: C
authors.lastname: Wang
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources of State Oceanic Administration, Third Institute of Oceanography, State Oceanic Administration, Key Laboratory of Marine Genetic Resources of Fujian Province, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen, 361005, PR China; School of Life Science, Xiamen University, Xiamen, 361005, PR China.
authors.forename: Xun
authors.initials: X
authors.lastname: Xu
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources of State Oceanic Administration, Third Institute of Oceanography, State Oceanic Administration, Key Laboratory of Marine Genetic Resources of Fujian Province, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen, 361005, PR China. Electronic address: ruanlingwei@tio.org.cn.
authors.forename: Lingwei
authors.initials: L
authors.lastname: Ruan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D033561:Penaeidae / Q000145:classification / Q000235:genetics* / Q000276:immunology* / Q000821:virology; D010802:Phylogeny; D015854:Up-Regulation; D046848:White spot syndrome virus 1 / Q000502:physiology
chemical_list: D060829:Arthropod Proteins; D018076:DNA, Complementary; D008565:Membrane Proteins; C106354:flotillins
keywords: Endocytosis; Flotillin-2; Shrimp; WSSV