title: Apoptosis induced by boric anhydrite (B2O3) after partial hepatectomy in rat liver.
abstract: Boron is one of the important elements that have a cell-growth suppressing effect. The apoptotic effects of B(2)O(3) that were investigated in rats on liver regeneration following 70 % partial hepatectomy (PH). Wistar albino male rats were used and divided into 4 groups (n = 7). The Saline control groups were given only a single dose of saline; the B(2)O(3)-treated groups that were only given a single dose of 1800 mg.kg(-1) B(2)O(3) by means of intraperitoneal injections following hepatectomy. Three and 6 hours after surgical procedures, all the groups were dissected and liver tissue samples were taken from the groups for NF-κB for caspase-3 gene and protein levels investigation by RT-PCR and TaqMan Protein Assay and histological analyses by TUNEL assay. B(2)O(3)-treated animals were examined and it was observed that NF-κB levels were decreased; however, caspase-3 gene expression and protein levels were increased significantly. This study demonstrated that B(2)O(3) induces caspase-3 and inhibits NF-κB at the early stage of liver regeneration (Fig. 4, Ref. 26).
authors.forename: A
authors.initials: A
authors.lastname: Ozen
authors.forename: M
authors.initials: M
authors.lastname: Canbek
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001896:Boron Compounds / Q000494:pharmacology*; D053148:Caspase 3 / Q000032:analysis; D006498:Hepatectomy / Q000379:methods; D007274:Injections, Intraperitoneal; D008099:Liver / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D008115:Liver Regeneration / Q000187:drug effects*; D008297:Male; D016328:NF-kappa B / Q000032:analysis; D011184:Postoperative Period; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome
chemical_list: D001896:Boron Compounds; D016328:NF-kappa B; C042168:boron oxide; D053148:Caspase 3
keywords: NF-κB; RT-PCR; TUNEL assay.; TaqMan Protein Assay; caspase-3; liver regeneration