title: Flow-assisted basophil activation tests in immediate drug hypersensitivity: two decades of Antwerp experience.
abstract: The last two decades have witnessed that flow-assisted analysis of in vitro-activated basophils can constitute a valuable adjunct in the in vitro diagnostic approach of immediate drug hypersensitivity reactions (IDHR). This article summarises the current experience with the basophil activation test in the diagnosis of IDHR, with particular focus on allergy to curarising neuromuscular blocking agents, antibiotics (β-lactams and fluoroquinolones), iodinated radiocontrast media and opiates.
authors.affiliation: a Faculty of Medicine and Health Science, Department of Immunology, Allergology, Rheumatology , University of Antwerp, Antwerp University Hospital , 2610 Antwerpen , Belgium.
authors.forename: E A
authors.initials: EA
authors.lastname: Mangodt
authors.affiliation: a Faculty of Medicine and Health Science, Department of Immunology, Allergology, Rheumatology , University of Antwerp, Antwerp University Hospital , 2610 Antwerpen , Belgium.
authors.forename: A L
authors.initials: AL
authors.lastname: Van Gasse
authors.affiliation: a Faculty of Medicine and Health Science, Department of Immunology, Allergology, Rheumatology , University of Antwerp, Antwerp University Hospital , 2610 Antwerpen , Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Bastiaensen
authors.affiliation: a Faculty of Medicine and Health Science, Department of Immunology, Allergology, Rheumatology , University of Antwerp, Antwerp University Hospital , 2610 Antwerpen , Belgium.
authors.forename: I I
authors.initials: II
authors.lastname: Decuyper
authors.affiliation: a Faculty of Medicine and Health Science, Department of Immunology, Allergology, Rheumatology , University of Antwerp, Antwerp University Hospital , 2610 Antwerpen , Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Uyttebroek
authors.affiliation: a Faculty of Medicine and Health Science, Department of Immunology, Allergology, Rheumatology , University of Antwerp, Antwerp University Hospital , 2610 Antwerpen , Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Faber
authors.affiliation: a Faculty of Medicine and Health Science, Department of Immunology, Allergology, Rheumatology , University of Antwerp, Antwerp University Hospital , 2610 Antwerpen , Belgium.
authors.forename: V
authors.initials: V
authors.lastname: Sabato
authors.affiliation: a Faculty of Medicine and Health Science, Department of Immunology, Allergology, Rheumatology , University of Antwerp, Antwerp University Hospital , 2610 Antwerpen , Belgium.
authors.forename: C H
authors.initials: CH
authors.lastname: Bridts
authors.affiliation: b Faculty of Medicine and Health Science, Department of Pediatrics , University of Antwerp, Antwerp University Hospital , 2610 Antwerpen , Belgium.
authors.forename: M M
authors.initials: MM
authors.lastname: Hagendorens
authors.affiliation: a Faculty of Medicine and Health Science, Department of Immunology, Allergology, Rheumatology , University of Antwerp, Antwerp University Hospital , 2610 Antwerpen , Belgium.
authors.forename: L S
authors.initials: LS
authors.lastname: De Clerck
authors.affiliation: a Faculty of Medicine and Health Science, Department of Immunology, Allergology, Rheumatology , University of Antwerp, Antwerp University Hospital , 2610 Antwerpen , Belgium.
authors.forename: D G
authors.initials: DG
authors.lastname: Ebo
mesh_terms: D000900:Anti-Bacterial Agents / Q000009:adverse effects; D001491:Basophils; D001530:Belgium; D004342:Drug Hypersensitivity; D005434:Flow Cytometry; D006801:Humans; D006969:Hypersensitivity, Immediate
chemical_list: D000900:Anti-Bacterial Agents
keywords: Allergy; Antibiotics; Basophil activation test (BAT), drug; CD203c; CD63; Curares; HistaFlow; Immediate drug hypersensitivity reaction (IDHR); Opiates