title: Genome Wide Host Gene Expression Analysis in Chicken Lungs Infected with Avian Influenza Viruses.
abstract: The molecular pathogenesis of avian influenza infection varies greatly with individual bird species and virus strain. The molecular pathogenesis of the highly pathogenic avian influenza virus (HPAIV) or the low pathogenic avian influenza virus (LPAIV) infection in avian species remains poorly understood. Thus, global immune response of chickens infected with HPAI H5N1 (A/duck/India/02CA10/2011) and LPAI H9N2 (A/duck/India/249800/2010) viruses was studied using microarray to identify crucial host genetic components responsive to these infection. HPAI H5N1 virus induced excessive expression of type I IFNs (IFNA and IFNG), cytokines (IL1B, IL18, IL22, IL13, and IL12B), chemokines (CCL4, CCL19, CCL10, and CX3CL1) and IFN stimulated genes (OASL, MX1, RSAD2, IFITM5, IFIT5, GBP 1, and EIF2AK) in lung tissues. This dysregulation of host innate immune genes may be the critical determinant of the severity and the outcome of the influenza infection in chickens. In contrast, the expression levels of most of these genes was not induced in the lungs of LPAI H9N2 virus infected chickens. This study indicated the relationship between host immune genes and their roles in pathogenesis of HPAIV infection in chickens.
authors.affiliation: Pathogenomics Laboratory, ICAR -National Institute of High Security Animal Diseases, OIE Reference lab for Avian Influenza, Bhopal, Madhya Pradesh, India.
authors.forename: Pradip B
authors.initials: PB
authors.lastname: Ranaware
authors.affiliation: Pathogenomics Laboratory, ICAR -National Institute of High Security Animal Diseases, OIE Reference lab for Avian Influenza, Bhopal, Madhya Pradesh, India.
authors.forename: Anamika
authors.initials: A
authors.lastname: Mishra
authors.affiliation: Pathogenomics Laboratory, ICAR -National Institute of High Security Animal Diseases, OIE Reference lab for Avian Influenza, Bhopal, Madhya Pradesh, India.
authors.forename: Periyasamy
authors.initials: P
authors.lastname: Vijayakumar
authors.affiliation: Pathogenomics Laboratory, ICAR -National Institute of High Security Animal Diseases, OIE Reference lab for Avian Influenza, Bhopal, Madhya Pradesh, India.
authors.forename: Pradeep N
authors.initials: PN
authors.lastname: Gandhale
authors.affiliation: Laboratory of Immunology, Department of Biological Sciences, Indian Institute of Science Education and Research, Bhopal, Madhya Pradesh, India.
authors.forename: Himanshu
authors.initials: H
authors.lastname: Kumar
authors.affiliation: Pathogenomics Laboratory, ICAR -National Institute of High Security Animal Diseases, OIE Reference lab for Avian Influenza, Bhopal, Madhya Pradesh, India.
authors.forename: Diwakar D
authors.initials: DD
authors.lastname: Kulkarni
authors.affiliation: Pathogenomics Laboratory, ICAR -National Institute of High Security Animal Diseases, OIE Reference lab for Avian Influenza, Bhopal, Madhya Pradesh, India.
authors.forename: Ashwin Ashok
authors.identifier: http://orcid.org/0000-0002-4978-901X
authors.initials: AA
authors.lastname: Raut
mesh_terms: D000818:Animals; D002645:Chickens / Q000821:virology*; D020869:Gene Expression Profiling; D023281:Genomics; D053124:Influenza A Virus, H5N1 Subtype / Q000502:physiology*; D053127:Influenza A Virus, H9N2 Subtype / Q000502:physiology*; D008168:Lung / Q000378:metabolism* / Q000821:virology*; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype