title: Isolation and characterization of a novel mycovirus from Penicillium digitatum.
abstract: A novel double-stranded RNA virus designated Penicillium digitatum virus 1 (PdV1) was isolated from the citrus fruit rot pathogen P. digitatum (HS-RH1). The full-length cDNA sequence of the dsRNA/PdV1 (5211bp) possesses two partially overlapping open reading frames, which encode a coat protein (CP) and a putative RNA-dependent RNA polymerase (RdRp), respectively. Phylogenetic analysis based on multiple alignments of the amino acid sequences of the RdRp and CP indicated that PdV1 tentatively belongs to the genus Victorivirus in the Totiviridae family. Electron micrographs of negatively stained viral particles purified from the peak fraction of sucrose density gradient centrifugation showed spherical particles ~35nm in diameter. Transfection experiments with purified virions indicated that PdV1 could reduce the vegetative growth and virulence of P. digitatum strain HS-F6. In summary, we report the first isolation and characterization of a mycovirus from P. digitatum that contributes to the hypovirulence phenotypes of the host strain.
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Science, Central China Normal University, Wuhan 430079, China.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Niu
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Science, Central China Normal University, Wuhan 430079, China.
authors.forename: Tingfu
authors.initials: T
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Science, Central China Normal University, Wuhan 430079, China.
authors.forename: Yongze
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Science, Central China Normal University, Wuhan 430079, China.
authors.forename: Shengqiang
authors.initials: S
authors.lastname: Wang
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Science, Central China Normal University, Wuhan 430079, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Science, Central China Normal University, Wuhan 430079, China. Electronic address: ldl@mail.ccnu.edu.cn.
authors.forename: Deli
authors.initials: D
authors.lastname: Liu
mesh_terms: D020121:5' Untranslated Regions; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001483:Base Sequence; D000067148:Fungal Viruses / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D010407:Penicillium / Q000821:virology*; D010802:Phylogeny; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D017422:Sequence Analysis, DNA; D014771:Virion / Q000648:ultrastructure
chemical_list: D020121:5' Untranslated Regions; D012330:RNA, Double-Stranded; D012367:RNA, Viral
keywords: Hypovirulence; PdV1; Penicillium digitatum; Transfection; Victorivirus in Totiviridae; dsRNA mycovirus