title: Prokaryotic Production of Virus-Like Particle Vaccine of Betanodavirus.
abstract: Betanodaviruses are the causative agents of viral nervous necrosis (VNN), a serious disease of cultured marine fish worldwide. To control this disease, vaccines of subunit capsid proteins (recombinant proteins or peptides), inactivated viruses, and virus-like particles (VLPs) were developed. VLP, which is highly similar to the wild-type virus in virion structure and contains no viral genome, was proved as one of the good and safe vaccines that can activate humoral immune response in the long term and induce cellular and innate immunities in the early stage post-immunization. The VLP vaccines can be expressed in vitro either by Baculovirus-based or yeast-based eukaryotic system or by bacterial expression system. In this chapter, the prokaryotic expression and the subsequent purification of VLP of betanodavirus orange-spotted grouper nervous necrosis virus (OGNNV) are presented.
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, No. 132 Waihuan Dong Road, Guangzhou, 510006, China. xiejf@mail.sysu.edu.cn.|MOE Key Laboratory of Aquatic Product Safety, School of Marine Sciences, Sun Yat-sen University, Guangzhou, 510006, China. xiejf@mail.sysu.edu.cn.|Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, No. 135 Xingang Xi Road, Guangzhou, 510275, China. xiejf@mail.sysu.edu.cn.|Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China. xiejf@mail.sysu.edu.cn.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, No. 132 Waihuan Dong Road, Guangzhou, 510006, China.|MOE Key Laboratory of Aquatic Product Safety, School of Marine Sciences, Sun Yat-sen University, Guangzhou, 510006, China.|Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, No. 135 Xingang Xi Road, Guangzhou, 510275, China.|Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China.
authors.forename: Runqing
authors.initials: R
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, No. 132 Waihuan Dong Road, Guangzhou, 510006, China.|MOE Key Laboratory of Aquatic Product Safety, School of Marine Sciences, Sun Yat-sen University, Guangzhou, 510006, China.|Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, No. 135 Xingang Xi Road, Guangzhou, 510275, China.|Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China.
authors.forename: Yuxiong
authors.initials: Y
authors.lastname: Lai
mesh_terms: D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D008958:Models, Molecular; D008968:Molecular Conformation; D030381:Nodaviridae / Q000276:immunology*; D016133:Polymerase Chain Reaction; D013010:Sonication; D058425:Vaccines, Virus-Like Particle / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*
chemical_list: D017931:DNA Primers; D058425:Vaccines, Virus-Like Particle
keywords: Betanodavirus; Prokaryotic expression; Vaccine purification; Virus-like particle