title: Reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid diagnosis of chilli veinal mottle virus.
abstract: Chilli veinal mottle virus (ChiVMV) causes significant economic loss to chilli cultivation in northeastern India, as well as in eastern Asia. In this study, we have developed a single-tube one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid, sensitive and specific diagnosis of ChiVMV. Amplification could be visualized after adding SYBR Green I (1000×) dye within 60 min under isothermal conditions at 63 °C, with a set of four primers designed based on the large nuclear inclusion protein (NIb) domain of ChiVMV (isolate KC-ML1). The RT-LAMP method was 100 times more sensitive than one-step reverse transcription polymerase chain reaction (RT-PCR), with a detection limit of 0.0001 ng of total RNA per reaction.
authors.affiliation: ICAR Research Complex for North Eastern Hill Region, Umiam, Meghalaya, 793 103, India. amrita.ars@gmail.com.
authors.forename: Amrita
authors.initials: A
authors.lastname: Banerjee
authors.affiliation: ICAR-National Bureau of Plant Genetic Resources, Regional Station, Umiam, Meghalaya, 793 103, India.
authors.forename: Somnath
authors.initials: S
authors.lastname: Roy
authors.affiliation: ICAR Research Complex for NEH Region, Manipur Centre, Lamphelpat, Manipur, 795 004, India.
authors.forename: Susheel Kumar
authors.initials: SK
authors.lastname: Sharma
authors.affiliation: ICAR Research Complex for NEH Region, Mizoram Centre, Kolasib, Mizoram, 796 081, India.
authors.forename: Sudip Kumar
authors.initials: SK
authors.lastname: Dutta
authors.affiliation: ICAR Research Complex for North Eastern Hill Region, Umiam, Meghalaya, 793 103, India.
authors.forename: Satish
authors.initials: S
authors.lastname: Chandra
authors.affiliation: ICAR Research Complex for North Eastern Hill Region, Umiam, Meghalaya, 793 103, India.
authors.forename: S V
authors.initials: SV
authors.lastname: Ngachan
mesh_terms: D002212:Capsicum / Q000821:virology*; D017931:DNA Primers / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010935:Plant Diseases / Q000821:virology*; D017800:Potyvirus / Q000145:classification / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D048348:Reverse Transcription; D012680:Sensitivity and Specificity; D014764:Viral Proteins / Q000235:genetics
chemical_list: D017931:DNA Primers; D014764:Viral Proteins