title: Effects of glucose metabolism pathways on sperm motility and oxidative status during long-term liquid storage of goat semen.
abstract: Although great efforts were made to prolong the fertility of liquid-stored semen, limited improvements have been achieved in different species. Although it is expected that energy supply and the redox potential will play an essential role in sperm function, there are few reports on the impact of specific energy substrates on spermatozoa during liquid semen storage. Furthermore, although it is accepted that glucose metabolism through glycolysis provides energy, roles of pentose phosphate pathway (PPP) and tricarboxylic acid cycle remain to be unequivocally found in spermatozoa. We have studied the pathways by which spermatozoa metabolize glucose during long-term liquid storage of goat semen. The results indicated that among the substrates tested, glucose and pyruvate were better than lactate in maintaining goat sperm motility. Although both glycolysis and PPP were essential, PPP was more important than glycolysis to maintain sperm motility. Pentose phosphate pathway reduced oxidative stress and provided glycolysis with more intermediate products such as fructose-6-phosphate. Pyruvate entered goat spermatozoa through monocarboxylate transporters and was oxidized by the tricarboxylic acid cycle and electron transfer to sustain sperm motility. Long-term liquid semen storage can be used as a good model to study sperm glucose metabolism. The data are important for an optimal control of sperm survival during semen handling and preservation not only in the goat but also in other species.
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai-an City, P. R. China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Qiu
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin, P. R. China.
authors.forename: You-Wei
authors.initials: YW
authors.lastname: Li
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai-an City, P. R. China.
authors.forename: Hong-Li
authors.initials: HL
authors.lastname: Xie
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai-an City, P. R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai-an City, P. R. China.
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Dong
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin, P. R. China.
authors.forename: Ming-Ju
authors.initials: MJ
authors.lastname: Sun
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai-an City, P. R. China.
authors.forename: Wei-Qiang
authors.initials: WQ
authors.lastname: Gao
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai-an City, P. R. China; College of Life Science, Northeast Agricultural University, Harbin, P. R. China. Electronic address: tanjh@sdau.edu.cn.
authors.forename: Jing-He
authors.initials: JH
authors.lastname: Tan
mesh_terms: D000818:Animals; D045222:Electron Transport Chain Complex Proteins; D005947:Glucose / Q000378:metabolism*; D006041:Goats / Q000502:physiology*; D008297:Male; D027501:Monocarboxylic Acid Transporters; D018384:Oxidative Stress / Q000502:physiology*; D019289:Pyruvic Acid / Q000378:metabolism; D012661:Semen / Q000502:physiology*; D012662:Semen Preservation / Q000662:veterinary; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*; D013696:Temperature; D013997:Time Factors
chemical_list: D045222:Electron Transport Chain Complex Proteins; D027501:Monocarboxylic Acid Transporters; D019289:Pyruvic Acid; D005947:Glucose
keywords: Glucose metabolism; Goat; Liquid semen storage; Semen; Spermatozoa