title: Authentication of Closely Related Fish and Derived Fish Products Using Tandem Mass Spectrometry and Spectral Library Matching.
abstract: Proteomics methodology has seen increased application in food authentication, including tandem mass spectrometry of targeted species-specific peptides in raw, processed, or mixed food products. We have previously described an alternative principle that uses untargeted data acquisition and spectral library matching, essentially spectral counting, to compare and identify samples without the need for genomic sequence information in food species populations. Here, we present an interlaboratory comparison demonstrating how a method based on this principle performs in a realistic context. We also increasingly challenge the method by using data from different types of mass spectrometers, by trying to distinguish closely related and commercially important flatfish, and by analyzing heavily contaminated samples. The method was found to be robust in different laboratories, and 94-97% of the analyzed samples were correctly identified, including all processed and contaminated samples.
authors.affiliation: RIKILT Wageningen UR , P.O. Box 230, 6700 AE Wageningen, The Netherlands.
authors.forename: Merel A
authors.initials: MA
authors.lastname: Nessen
authors.affiliation: RIKILT Wageningen UR , P.O. Box 230, 6700 AE Wageningen, The Netherlands.
authors.forename: Dennis J
authors.initials: DJ
authors.lastname: van der Zwaan
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center , P.O. Box 9600, 2300 RC Leiden, The Netherlands.
authors.forename: Sander
authors.initials: S
authors.lastname: Grevers
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center , P.O. Box 9600, 2300 RC Leiden, The Netherlands.
authors.forename: Hans
authors.initials: H
authors.lastname: Dalebout
authors.affiliation: RIKILT Wageningen UR , P.O. Box 230, 6700 AE Wageningen, The Netherlands.
authors.forename: Martijn
authors.initials: M
authors.lastname: Staats
authors.affiliation: RIKILT Wageningen UR , P.O. Box 230, 6700 AE Wageningen, The Netherlands.
authors.forename: Esther
authors.initials: E
authors.lastname: Kok
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center , P.O. Box 9600, 2300 RC Leiden, The Netherlands.
authors.forename: Magnus
authors.initials: M
authors.lastname: Palmblad
mesh_terms: D000818:Animals; D005396:Fish Products / Q000032:analysis* / Q000145:classification; D005399:Fishes / Q000145:classification / Q000235:genetics*; D005506:Food Contamination / Q000032:analysis*; D015723:Gene Library; D013045:Species Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
keywords: food authentication; mass spectrometry; proteomics; species identification; spectral libraries