title: Novel Alginate-Chitosan Composite Microspheres for Implant Delivery of Vancomycin and In Vivo Evaluation.
abstract: In this study, vancomycin loaded alginate-chitosan composite microspheres were developed by emulsion cross-linking method. The in vitro and vivo characterizations were done to evaluate the feasibility of application. Our experimental results showed that the emulsification cross-linking technique appeared to be a feasible method for the preparation of alginate-chitosan composite microspheres. The microspheres were spherical in shape and the mean particle size and drug loading were 25.3 ± 5.4 μm and 18.5 ± 2.3% respectively. A sustained vancomycin release was realized i.e. the amount of cumulative release increased in a time frame of 24 h to reach an amount i.e. ~68%. The model that fit best for vancomycin released from the microspheres was the Higuchi kinetic model with a correlation coefficient r = 0.9996. In vivo results showed that the application of microspheres not only reduced the toxicity, but also maintained effective drug concentration. In addition, no severe signs of epithelial necrosis and sloughing of epithelial cells were detected in histological studies.
authors.affiliation: Department of Thoracic Surgery, Xiaoshan Hospital of Traditional Chinese Medicine, 156 Yu Cai Road, Xiaoshan District, Hangzhou 311201, China.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Department of Thoracic Surgery, Huai'an First People's Hospital, 6 Beijing west Road Huai'an, Jiangsu 223300, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Department of Thoracic Surgery, Ninghai First People's Hospital, 6 Taoyuan middle Road, Ninghai 315600, China.
authors.forename: Yongbiao
authors.initials: Y
authors.lastname: Ge
authors.affiliation: Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University School of Medicine, 507 Zheng Min Road, Yangpu, Shanghai 200433, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Fan
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D048271:Chitosan / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004343:Drug Implants; D005260:Female; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D008297:Male; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D011817:Rabbits; D014640:Vancomycin / Q000008:administration & dosage* / Q000493:pharmacokinetics
chemical_list: D000464:Alginates; D000900:Anti-Bacterial Agents; D004343:Drug Implants; D006603:Hexuronic Acids; D014640:Vancomycin; D020723:Glucuronic Acid; D048271:Chitosan
keywords: alginate-chitosan composite microspheres; in vitro release; pharmacokinetics; vancomycin