title: Integrative transcriptome, proteome, phosphoproteome and genetic mapping reveals new aspects in a fiberless mutant of cotton.
abstract: To investigate the molecular mechanisms of fiber initiation in cotton (Gossypium spp.), an integrated approach combining transcriptome, iTRAQ-based proteome and genetic mapping was taken to compare the ovules of the Xuzhou 142 wild type (WT) with its fuzzless-lintless (fl) mutant at -3 and 0 day post-anthesis. A total of 1,953 mRNAs, 187 proteins, and 131 phosphoproteins were differentially expressed (DE) between WT and fl, and the levels of transcripts and their encoded proteins and phosphoproteins were highly congruent. A functional analysis suggested that the abundance of proteins were mainly involved in amino sugar, nucleotide sugar and fatty acid metabolism, one carbon pool for folate metabolism and flavonoid biosynthesis. qRT-PCR, Western blotting, and enzymatic assays were performed to confirm the regulation of these transcripts and proteins. A molecular mapping located the lintless gene li3 in the fl mutant on chromosome 26 for the first time. A further in-silico physical mapping of DE genes with sequence variations between fl and WT identified one and four candidate genes in the li3 and n2 regions, respectively. Taken together, the transcript abundance, phosphorylation status of proteins at the fiber initiation stage and candidate genes have provided insights into regulatory processes underlying cotton fiber initiation.
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, Henan 455000, China.
authors.forename: Qi-Feng
authors.initials: QF
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, Henan 455000, China.
authors.forename: Chun-Hui
authors.initials: CH
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, Henan 455000, China.
authors.forename: Man
authors.initials: M
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, Henan 455000, China.
authors.forename: Wen-Feng
authors.initials: WF
authors.lastname: Pei
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, Henan 455000, China.
authors.forename: Xing-Li
authors.initials: XL
authors.lastname: Li
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, Henan 455000, China.
authors.forename: Wen-Kui
authors.initials: WK
authors.lastname: Wang
authors.affiliation: Department of Plant and Environmental Sciences, New Mexico State University, Las Cruces, NM 88003, USA.
authors.forename: Jinfa
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, Henan 455000, China.
authors.forename: Ji-Wen
authors.initials: JW
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, Henan 455000, China.
authors.forename: Shu-Xun
authors.initials: SX
authors.lastname: Yu
mesh_terms: D015153:Blotting, Western; D032561:Cotton Fiber; D003600:Cytosol / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D003368:Gossypium / Q000737:chemistry* / Q000235:genetics*; D009154:Mutation; D010750:Phosphoproteins / Q000032:analysis*; D020161:Physical Chromosome Mapping; D020543:Proteome / Q000032:analysis*; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome
chemical_list: D005227:Fatty Acids; D010750:Phosphoproteins; D020543:Proteome