title: Clinicopathological Analysis and Multipronged Quantitative Proteomics Reveal Oxidative Stress and Cytoskeletal Proteins as Possible Markers for Severe Vivax Malaria.
abstract: In Plasmodium vivax malaria, mechanisms that trigger transition from uncomplicated to fatal severe infections are obscure. In this multi-disciplinary study we have performed a comprehensive analysis of clinicopathological parameters and serum proteome profiles of vivax malaria patients with different severity levels of infection to investigate pathogenesis of severe malaria and identify surrogate markers of severity. Clinicopathological analysis and proteomics profiling has provided evidences for the modulation of diverse physiological pathways including oxidative stress, cytoskeletal regulation, lipid metabolism and complement cascades in severe malaria. Strikingly, unlike severe falciparum malaria the blood coagulation cascade was not found to be affected adversely in acute P. vivax infection. To the best of our knowledge, this is the first comprehensive proteomics study, which identified some possible cues for severe P. vivax infection. Our results suggest that Superoxide dismutase, Vitronectin, Titin, Apolipoprotein E, Serum amyloid A, and Haptoglobin are potential predictive markers for malaria severity.
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors.forename: Sandipan
authors.initials: S
authors.lastname: Ray
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors.forename: Sandip K
authors.initials: SK
authors.lastname: Patel
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors.forename: Apoorva
authors.initials: A
authors.lastname: Venkatesh
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors.forename: Amruta
authors.initials: A
authors.lastname: Bhave
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors.forename: Vipin
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors.forename: Vaidhvi
authors.initials: V
authors.lastname: Singh
authors.affiliation: Dept of Biochemistry, Grant Govt Medical College and Sir JJ Group of Hospitals, Byculla, Mumbai 400008, India.
authors.forename: Gangadhar
authors.initials: G
authors.lastname: Chatterjee
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.|Wipro GE Healthcare, Mumbai, India.
authors.forename: Veenita G
authors.initials: VG
authors.lastname: Shah
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors.forename: Sarthak
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Strand Life Sciences Pvt. Ltd., Hebbal, Bangalore 560024, India.
authors.forename: Durairaj
authors.initials: D
authors.lastname: Renu
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors.forename: Naziya
authors.initials: N
authors.lastname: Nafis
authors.affiliation: Departments of Clinical Pharmacology, Seth GS Medical College &KEM Hospital, Parel, Mumbai 400012, India.
authors.forename: Prajakta
authors.initials: P
authors.lastname: Gandhe
authors.affiliation: Departments of Clinical Pharmacology, Seth GS Medical College &KEM Hospital, Parel, Mumbai 400012, India.
authors.forename: Nithya
authors.initials: N
authors.lastname: Gogtay
authors.affiliation: Departments of Clinical Pharmacology, Seth GS Medical College &KEM Hospital, Parel, Mumbai 400012, India.
authors.forename: Urmila
authors.initials: U
authors.lastname: Thatte
authors.affiliation: PD Hinduja National Hospital &Medical Research Centre, Veer Savarkar Marg, Mahim, Mumbai 400016, India.
authors.forename: Kunal
authors.initials: K
authors.lastname: Sehgal
authors.affiliation: Medicine Department, Medical College Hospital Kolkata, 88, College Street, Kolkata 700073, India.
authors.forename: Sumit
authors.initials: S
authors.lastname: Verma
authors.affiliation: Medicine Department, Medical College Hospital Kolkata, 88, College Street, Kolkata 700073, India.
authors.forename: Avik
authors.initials: A
authors.lastname: Karak
authors.affiliation: Medicine Department, Medical College Hospital Kolkata, 88, College Street, Kolkata 700073, India.
authors.forename: Dibbendhu
authors.initials: D
authors.lastname: Khanra
authors.affiliation: Medicine Department, Medical College Hospital Kolkata, 88, College Street, Kolkata 700073, India.
authors.forename: Arunansu
authors.initials: A
authors.lastname: Talukdar
authors.affiliation: Department of Medicine, Malaria Research Center, S.P. Medical College, Bikaner 334003, India.
authors.forename: Sanjay K
authors.initials: SK
authors.lastname: Kochar
authors.affiliation: Department of Medicine, Malaria Research Center, S.P. Medical College, Bikaner 334003, India.
authors.forename: Vijeth
authors.initials: V
authors.lastname: S B
authors.affiliation: Department of Medicine, Malaria Research Center, S.P. Medical College, Bikaner 334003, India.|Department of Medicine, RUHS College of Medical Sciences, Jaipur, Rajasthan 302033, India.
authors.forename: Dhanpat K
authors.initials: DK
authors.lastname: Kochar
authors.affiliation: Department of Medicine, Malaria Research Center, S.P. Medical College, Bikaner 334003, India.
authors.forename: Dharmendra
authors.initials: D
authors.lastname: Rojh
authors.affiliation: Dept of Biochemistry, Grant Govt Medical College and Sir JJ Group of Hospitals, Byculla, Mumbai 400008, India.
authors.forename: Santosh G
authors.initials: SG
authors.lastname: Varma
authors.affiliation: Centre for Research in Nanotechnology &Science, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors.forename: Mayuri N
authors.initials: MN
authors.lastname: Gandhi
authors.affiliation: Proteomics Laboratory, National Centre for Cell Science, Ganeshkhind, Pune 411007, India.
authors.forename: Rapole
authors.initials: R
authors.lastname: Srikanth
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors.forename: Swati
authors.initials: S
authors.lastname: Patankar
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors.forename: Sanjeeva
authors.initials: S
authors.lastname: Srivastava
mesh_terms: D000328:Adult; D001057:Apolipoproteins E / Q000097:blood; D015415:Biomarkers / Q000097:blood*; D064211:Connectin / Q000097:blood; D003598:Cytoskeletal Proteins / Q000097:blood*; D005260:Female; D006242:Haptoglobins / Q000378:metabolism; D006801:Humans; D016780:Malaria, Vivax / Q000097:blood* / Q000469:parasitology; D018384:Oxidative Stress; D010966:Plasmodium vivax / Q000472:pathogenicity; D040901:Proteomics; D000685:Serum Amyloid A Protein / Q000378:metabolism; D013482:Superoxide Dismutase / Q000097:blood; D019096:Vitronectin / Q000097:blood
chemical_list: D001057:Apolipoproteins E; D015415:Biomarkers; D064211:Connectin; D003598:Cytoskeletal Proteins; D006242:Haptoglobins; D000685:Serum Amyloid A Protein; D019096:Vitronectin; D013482:Superoxide Dismutase