title: Butanol production employing fed-batch fermentation by Clostridium acetobutylicum GX01 using alkali-pretreated sugarcane bagasse hydrolysed by enzymes from Thermoascus aurantiacus QS 7-2-4.
abstract: Sugarcane bagasse (SB) is a potential feedstock for butanol production. However, biological production of butanol from SB is less economically viable. In this study, evaluation of eight pretreatments on SB showed that alkali pretreatment efficiently removed lignin from SB while retaining the intact native structure of the released microfibrils. In total, 99% of cellulose and 100% of hemicellulose in alkali-pretreated SB were hydrolysed by enzymes from Thermoascus aurantiacus. The hydrolysate was used to produce butanol in a fed-batch fermentation by Clostridium acetobutylicum. At 60h, 14.17 and 21.11gL(-1) of butanol and acetone-butanol-ethanol (ABE) were produced from 68.89gL(-1) of total sugars, respectively, yielding 0.22 and 0.33gg(-1) of sugars. The maximum yield of butanol and ABE reached 15.4g and 22.9g per 100g raw SB, respectively. This established process may have potential application for butanol production from SB.
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China.
authors.forename: Zong-Wen
authors.initials: ZW
authors.lastname: Pang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China.
authors.forename: Zheng-Wu
authors.initials: ZW
authors.lastname: Liang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China. Electronic address: jjliang@gxu.edu.cn.
authors.forename: Jing-Juan
authors.initials: JJ
authors.lastname: Liang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China.
authors.forename: Liang-Wei
authors.initials: LW
authors.lastname: Du
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China.
authors.forename: Cheng-Jie
authors.initials: CJ
authors.lastname: Duan
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China.
authors.forename: Jia-Xun
authors.initials: JX
authors.lastname: Feng
mesh_terms: D000096:Acetone / Q000378:metabolism; D000468:Alkalies / Q000494:pharmacology*; D061249:Batch Cell Culture Techniques / Q000379:methods*; D019149:Bioreactors / Q000382:microbiology; D000440:Butanols / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry*; D046969:Clostridium acetobutylicum / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D006868:Hydrolysis; D007700:Kinetics; D031786:Saccharum / Q000737:chemistry* / Q000187:drug effects; D013696:Temperature; D055327:Thermoascus / Q000201:enzymology*; D013997:Time Factors
chemical_list: D000468:Alkalies; D000440:Butanols; D000096:Acetone; D000431:Ethanol; D002482:Cellulose; C027433:bagasse
keywords: Alkali pretreatment; Butanol; Fed-batch fermentation; Sugarcane bagasse