title: Metaproteomics Applied to Activated Sludge for Industrial Wastewater Treatment Revealed a Dominant Methylotrophic Metabolism of Hyphomicrobium zavarzinii.
abstract: In biological wastewater treatments, microbial populations of the so-called activated sludge work together in the abatement of pollutants. In this work, the metabolic behavior of the biomass of a lab-scale plant treating industrial pharmaceutical wastewater was investigated through a metaproteomic approach. The complete treatment process included a membrane biological reactor (MBR) coupled with an advanced oxidation process (AOP) for partial breakdown of non-biodegradable molecules. Proteins from biomass samples collected pre- and post-AOP application were investigated by two-dimensional gel electrophoresis (2DE), mass spectrometry (MS), and finally identified by database search. Results showed that most proteins remained constant between pre- and post-AOP. Methanol dehydrogenase (MDH) belonging to Hyphomicrobium zavarzinii appeared as the most constantly expressed protein in the studied consortium. Other identified proteins belonging to Hyphomicrobium spp. revealed a predominant methylotrophic metabolism, and H. zavarzinii appeared as a key actor in the studied microbial community.
authors.affiliation: Water Research Institute, Viale F. De Blasio 5, 70132, Bari, Italy. carlo.salerno@ba.irsa.cnr.it.
authors.forename: Carlo
authors.initials: C
authors.lastname: Salerno
authors.affiliation: Max Planck Institute for Dynamics of Complex Technical Systems, Bioprocess Engineering, Sandtorstraße 1, 39106, Magdeburg, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Benndorf
authors.affiliation: Max Planck Institute for Dynamics of Complex Technical Systems, Bioprocess Engineering, Sandtorstraße 1, 39106, Magdeburg, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Kluge
authors.affiliation: Department of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari, Policlinico, P.zza G. Cesare 11, 70124, Bari, Italy.
authors.forename: Luigi Leonardo
authors.initials: LL
authors.lastname: Palese
authors.affiliation: Otto-von-Guericke University, Bioprocess Engineering, Universitätsplatz 2, 39106, Magdeburg, Germany.
authors.forename: Udo
authors.initials: U
authors.lastname: Reichl
authors.affiliation: Water Research Institute, Viale F. De Blasio 5, 70132, Bari, Italy.
authors.forename: Alfieri
authors.initials: A
authors.lastname: Pollice
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism; D018533:Biomass; D020582:Hyphomicrobium / Q000302:isolation & purification / Q000378:metabolism*; D040901:Proteomics; D012722:Sewage / Q000737:chemistry / Q000382:microbiology*; D053719:Tandem Mass Spectrometry; D018505:Waste Management / Q000379:methods*
chemical_list: D012722:Sewage; D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor)
keywords: Activated sludge; Hyphomicrobium; Metaproteomics; Methanol dehydrogenase; Wastewater