title: Chemiluminescence analysis of antioxidant capacity for serum albumin isolated from healthy or uremic volunteers.
abstract: Regular hemodialysis treatment induces an elevation in oxidative stress in patients with end-stage renal failure, resulting in oxidative damage of the most abundant serum protein, albumin. Oxidation of serum albumin causes depletion of albumin reactive thiols, leading to oxidative modification of serum albumin. The aim of this study was to screen the antioxidant capacity of albumins isolated from uremic patients (HD-ALB) or healthy volunteers (N-ALB). From high-performance liquid chromatography spectra, we observed that one uremic solute binds to HD-ALB via the formation of disulfide bonds between HD-ALB and the uremic solute. Furthermore, we found using chemiluminescent analysis that the antioxidant capacities for N-ALB to scavenge reactive oxygen species including singlet oxygen, hypochlorite and hydrogen peroxide were higher than HD-ALB. Our results suggest that protein-bound uremic solute binds to albumin via formation of disulfide bonds, resulting in the depletion of albumin reactive thiols. The depletion of albumin reactive thiols leads to a reduced antioxidant capacity of HD-ALB, implying postmodification of albumin. This situation may reduce the antioxidant capacity of albumin and increase oxidative stress, resulting in increase in complications related to oxidative damage in uremic patients. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Graduate Institute of Basic Medical Science, China Medical University, Taichung, 404, Taiwan.|Department of Health and Nutrition Biotechnology, Asia University, Taichung, 413, Taiwan.|School of Chinese Medicine, China Medical University, Taichung, 404, Taiwan.
authors.forename: Chih-Yang
authors.initials: CY
authors.lastname: Huang
authors.affiliation: Formosan Blood Purification Foundation, 50, Sec. 1, Bade Rd., Taipei, Taiwan.
authors.forename: Show-Yih
authors.initials: SY
authors.lastname: Liou
authors.affiliation: Department of Biological Science and Technology, China Medical University, Taichung, 404, Taiwan.
authors.forename: Wei-Wen
authors.initials: WW
authors.lastname: Kuo
authors.affiliation: School of Medicine, China Medical University, Taichung, 404, Taiwan.
authors.forename: Hsi-Chin
authors.initials: HC
authors.lastname: Wu
authors.affiliation: Department of Biomedical Engineering, Chung Yuan Christian University, 200, Chung Pei Rd., Chung Li, Taiwan, 32023.
authors.forename: Yen-Lin
authors.initials: YL
authors.lastname: Chang
authors.affiliation: Graduate Institute of Basic Medical Science, China Medical University, Taichung, 404, Taiwan.|Biomaterials Translational Research Center, China Medical University Hospital, Taichung, 404, Taiwan.
authors.forename: Tung-Sheng
authors.initials: TS
authors.lastname: Chen
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D049449:Luminescence; D008297:Male; D018384:Oxidative Stress; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism; D014511:Uremia / Q000378:metabolism / Q000503:physiopathology*
chemical_list: D000975:Antioxidants; D012709:Serum Albumin
keywords: albumin oxidation; antioxidant capacity; oxidative stress; protein-bound uremic solutes; uremic patients