title: Cloning and molecular analysis of voraxin-α gene of Rhipicephalus (Boophilus) microplus.
abstract: To identify suitable targets for development of cross-protective tick vaccine, in silico analysis was attempted and male tick derived molecule, voraxin-α was targeted. The voraxin-α homologue of Rhipicephalus (Boophilus) microplus was cloned, sequenced and analyzed employing standard methods. The deduced amino acids sequence analysis of the 419 bp cloned voraxin-α gene of R. (B.) microplus indicated very high (94.6 %) similarity with voraxin-α of the R. appendiculatus and moderate to low identity with Amblyomma hebraeum, Dermacentor silvarum and Haemaphysalis longicornis. The results suggest that recombinant voraxin-α might be a good candidate as cross-protective anti-tick vaccine.
authors.affiliation: Entomology Laboratory, Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, 243122 India ; Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh, 362001 India.
authors.forename: Binod
authors.initials: B
authors.lastname: Kumar
authors.affiliation: Entomology Laboratory, Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, 243122 India.
authors.forename: Srikanta
authors.initials: S
authors.lastname: Ghosh
keywords: Anti-tick vaccine; Rhipicephalus (Boophilus) microplus; Voraxin-α