title: A 21-35 kDa Mixed Protein Component from Helicobacter pylori Activates Mast Cells Effectively in Chronic Spontaneous Urticaria.
abstract: BACKGROUND
  Helicobacter pylori (H. pylori) seem to involve in the etiology of chronic spontaneous urticaria (CSU). But studies of the pathogenic mechanism are very little.
  
  
  METHODS
  In this study, we detected the serum-specific anti-H. pylori IgG and IgE antibodies in 211 CSU and 137 normal subjects by enzyme-linked immunosorbent assay (ELISA), evaluated the direct activation effects of H. pylori preparations and its protein components on human LAD2mast cell line in vitro, and analyzed the specific protein ingredients and functions of the most effective H. pylori mixed protein component using liquid chromatography-mass spectrometry and ELISA assay.
  
  
  RESULTS
  In CSU patients, the positive rate of anti-H. pylori IgG positive rate was significantly higher than that in normal controls, and the anti-H. pylori IgE levels had no statistical difference between H. pylori-infected patients with and without CSU. Further studies suggested that H. pylori preparations can directly activate human LAD2mast cell line in a dose-dependent manner and its most powerful protein component was a mixture of 21-35 kDa proteins. Moreover, the 21-35 kDa mixed protein component mainly contained 23 kinds of proteins, which can stimulate the release of histamine, TNF-a, IL-3, IFN-γ, and LTB4 by LAD2cells in a dose-dependent or time-dependent manner.
  
  
  CONCLUSIONS
  A 21-35 kDa mixed protein component should be regarded as the most promising pathogenic factor contributing to the CSU associated with H. pylori infection.
authors.affiliation: Department of Dermatology, Daping Hospital, The Third Military Medical University, Chongqing, 400042, China.|Department of Microbiology and Biochemical Pharmacy, National Engineering Research Center of Immunological Products, College of Pharmacy, The Third Military Medical University, Chongqing, 400038, China.
authors.forename: Ran-Jing
authors.initials: RJ
authors.lastname: Tan
authors.affiliation: Department of Microbiology and Biochemical Pharmacy, National Engineering Research Center of Immunological Products, College of Pharmacy, The Third Military Medical University, Chongqing, 400038, China.
authors.forename: He-Qiang
authors.initials: HQ
authors.lastname: Sun
authors.affiliation: Department of Dermatology, Daping Hospital, The Third Military Medical University, Chongqing, 400042, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Microbiology and Biochemical Pharmacy, National Engineering Research Center of Immunological Products, College of Pharmacy, The Third Military Medical University, Chongqing, 400038, China.
authors.forename: Han-Mei
authors.initials: HM
authors.lastname: Yuan
authors.affiliation: Department of Microbiology and Biochemical Pharmacy, National Engineering Research Center of Immunological Products, College of Pharmacy, The Third Military Medical University, Chongqing, 400038, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Dermatology, Daping Hospital, The Third Military Medical University, Chongqing, 400042, China.
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Yan
authors.affiliation: Department of Gastroenterology, Daping Hospital, The Third Military Medical University, Chongqing, 400042, China.
authors.forename: Chun-Hui
authors.initials: CH
authors.lastname: Lan
authors.affiliation: Department of Gastroenterology, Daping Hospital, The Third Military Medical University, Chongqing, 400042, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Microbiology and Biochemical Pharmacy, National Engineering Research Center of Immunological Products, College of Pharmacy, The Third Military Medical University, Chongqing, 400038, China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Dermatology, Daping Hospital, The Third Military Medical University, Chongqing, 400042, China.
authors.forename: Jin-Jin
authors.initials: JJ
authors.lastname: Wu
authors.affiliation: Department of Microbiology and Biochemical Pharmacy, National Engineering Research Center of Immunological Products, College of Pharmacy, The Third Military Medical University, Chongqing, 400038, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology*; D002460:Cell Line; D002853:Chromatography, Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D013058:Mass Spectrometry; D008407:Mast Cells / Q000276:immunology*; D008875:Middle Aged; D008970:Molecular Weight; D017422:Sequence Analysis, DNA; D044967:Serum / Q000276:immunology; D014581:Urticaria / Q000209:etiology* / Q000473:pathology; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007074:Immunoglobulin G; D007073:Immunoglobulin E
keywords: 
  Helicobacter pylori
  ; Chronic spontaneous urticaria; activation effect; mast cells; mixed protein component