title: In situ enzyme aided adsorption of soluble xylan biopolymers onto cellulosic material.
abstract: The functional properties of cellulose fibers can be modified by adsorption of xylan biopolymers. The adsorption is improved when the degree of biopolymers substitution with arabinose and 4-O-methyl-glucuronic acid (MeGlcA) side groups, is reduced. α-l-Arabinofuranosidase (AbfB) and α-d-glucuronidase (AguA) enzymes were applied for side group removal, to increase adsorption of xylan from sugarcane (Saccharum officinarum L) bagasse (BH), bamboo (Bambusa balcooa) (BM), Pinus patula (PP) and Eucalyptus grandis (EH) onto cotton lint. The AguA treatment increased the adsorption of all xylans by up to 334%, whereas, the AbfB increased the adsorption of the BM and PP by 31% and 44%, respectively. A combination of AguA and AbfB treatment increased the adsorption, but to a lesser extent than achieved with AguA treatment. This indicated that the removal of the glucuronic acid side groups provided the most significant increase in xylan adsorption to cellulose, in particular through enzymatic treatment.
authors.affiliation: Department of Process Engineering, University of Stellenbosch, P/Bag X1, Stellenbosch 7602, South Africa. Electronic address: achimpha@sun.ac.za.
authors.forename: Annie F A
authors.initials: AF
authors.lastname: Chimphango
authors.affiliation: Department of Process Engineering, University of Stellenbosch, P/Bag X1, Stellenbosch 7602, South Africa.
authors.forename: J F
authors.initials: JF
authors.lastname: Görgens
authors.affiliation: Department of Microbiology, University of Stellenbosch, P/Bag X1, Stellenbosch 7602, South Africa.
authors.forename: W H
authors.initials: WH
authors.lastname: van Zyl
mesh_terms: D000327:Adsorption; D001089:Arabinose / Q000032:analysis; D002482:Cellulose / Q000737:chemistry*; D032561:Cotton Fiber; D005052:Eucalyptus; D005965:Glucuronates / Q000032:analysis; D006026:Glycoside Hydrolases / Q000737:chemistry*; D003368:Gossypium; D028223:Pinus; D031786:Saccharum; D031787:Sasa; D012567:Schizophyllum; D014990:Xylans / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D005965:Glucuronates; D014990:Xylans; C036035:4-O-methylglucuronic acid; C038910:glucuronoxylan; D002482:Cellulose; D001089:Arabinose; D006026:Glycoside Hydrolases; C096734:alpha-glucuronidase; C019713:alpha-N-arabinofuranosidase
keywords: Adsorption; Cellulosic material; Enzymes; Xylan biopolymer; α-d-glucuronidase; α-l-Arabinofuranosidase