title: Screen Identifying Arabidopsis Transcription Factors Involved in the Response to 9-Lipoxygenase-Derived Oxylipins.
abstract: 13-Lipoxygenase-derived oxylipins, such as jasmonates act as potent signaling molecules in plants. Although experimental evidence supports the impact of oxylipins generated by the 9-Lipoxygenase (9-LOX) pathway in root development and pathogen defense, their signaling function in plants remains largely elusive. Based on the root growth inhibiting properties of the 9-LOX-oxylipin 9-HOT (9-hydroxy-10,12,15-octadecatrienoic acid), we established a screening approach aiming at identifying transcription factors (TFs) involved in signaling and/or metabolism of this oxylipin. Making use of the AtTORF-Ex (Arabidopsis thaliana Transcription Factor Open Reading Frame Expression) collection of plant lines overexpressing TF genes, we screened for those TFs which restore root growth on 9-HOT. Out of 6,000 lines, eight TFs were recovered at least three times and were therefore selected for detailed analysis. Overexpression of the basic leucine Zipper (bZIP) TF TGA5 and its target, the monoxygenase CYP81D11 reduced the effect of added 9-HOT, presumably due to activation of a detoxification pathway. The highly related ETHYLENE RESPONSE FACTORs ERF106 and ERF107 induce a broad detoxification response towards 9-LOX-oxylipins and xenobiotic compounds. From a set of 18 related group S-bZIP factors isolated in the screen, bZIP11 is known to participate in auxin-mediated root growth and may connect oxylipins to root meristem function. The TF candidates isolated in this screen provide starting points for further attempts to dissect putative signaling pathways involving 9-LOX-derived oxylipins.
authors.affiliation: Julius-von-Sachs-Institute, University of Würzburg, Julius-von-Sachs-Platz 2, D-97082 Würzburg, Germany.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Walper
authors.affiliation: Julius-von-Sachs-Institute, University of Würzburg, Julius-von-Sachs-Platz 2, D-97082 Würzburg, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Weiste
authors.affiliation: Julius-von-Sachs-Institute, University of Würzburg, Julius-von-Sachs-Platz 2, D-97082 Würzburg, Germany.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Mueller
authors.affiliation: Division of Physiological Chemistry II, Department of Medical Biochemistry and Biophysics, Karolinska Institute, S-171 77 Stockholm, Sweden.
authors.forename: Mats
authors.initials: M
authors.lastname: Hamberg
authors.affiliation: Julius-von-Sachs-Institute, University of Würzburg, Julius-von-Sachs-Platz 2, D-97082 Würzburg, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Dröge-Laser
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant / Q000187:drug effects; D008042:Linolenic Acids / Q000494:pharmacology*; D054883:Oxylipins / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects* / Q000378:metabolism; D030821:Plants, Genetically Modified; D015398:Signal Transduction / Q000187:drug effects; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D008042:Linolenic Acids; D054883:Oxylipins; D014157:Transcription Factors; C062142:9-hydroxy-10,12,15-octadecatrienoic acid