title: Genipin cross-linked decellularized tracheal tubular matrix for tracheal tissue engineering applications.
abstract: Decellularization techniques have been widely used as an alternative strategy for organ reconstruction. This study investigated the mechanical, pro-angiogenic and in vivo biocompatibility properties of decellularized airway matrices cross-linked with genipin. New Zealand rabbit tracheae were decellularized and cross-linked with genipin, a naturally derived agent. The results demonstrated that, a significant (p < 0.05) increase in the secant modulus was computed for the cross-linked tracheae, compared to the decellularized samples. Angiogenic assays demonstrated that decellularized tracheal scaffolds and cross-linked tracheae treated with 1% genipin induce strong in vivo angiogenic responses (CAM analysis). Seven, 15 and 30 days after implantation, decreased (p < 0.01) inflammatory reactions were observed in the xenograft models for the genipin cross-linked tracheae matrices compared with control tracheae, and no increase in the IgM or IgG content was observed in rats. In conclusion, treatment with genipin improves the mechanical properties of decellularized airway matrices without altering the pro-angiogenic properties or eliciting an in vivo inflammatory response.
authors.affiliation: Department of Cardiothoracic Surgery, College of Clinical Medicine, Yangzhou University, Yangzhou, 225001, Jiangsu Province, China.|Yangzhou University Medical College, Yangzhou, 225001, Jiangsu Province, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Sun
authors.affiliation: Yangzhou University Medical College, Yangzhou, 225001, Jiangsu Province, China.|Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou, 225001, Jiangsu Province, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Yangzhou University Medical College, Yangzhou, 225001, Jiangsu Province, China.|Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou, 225001, Jiangsu Province, China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Cardiothoracic Surgery, College of Clinical Medicine, Yangzhou University, Yangzhou, 225001, Jiangsu Province, China.|Yangzhou University Medical College, Yangzhou, 225001, Jiangsu Province, China.|Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou, 225001, Jiangsu Province, China.
authors.forename: Hongcan
authors.initials: H
authors.lastname: Shi
authors.affiliation: Department of Cardiothoracic Surgery, College of Clinical Medicine, Yangzhou University, Yangzhou, 225001, Jiangsu Province, China.|Yangzhou University Medical College, Yangzhou, 225001, Jiangsu Province, China.|Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou, 225001, Jiangsu Province, China.
authors.forename: Siquan
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Cardiothoracic Surgery, College of Clinical Medicine, Yangzhou University, Yangzhou, 225001, Jiangsu Province, China.|Yangzhou University Medical College, Yangzhou, 225001, Jiangsu Province, China.
authors.forename: Xingchen
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Cardiothoracic Surgery, College of Clinical Medicine, Yangzhou University, Yangzhou, 225001, Jiangsu Province, China.|Yangzhou University Medical College, Yangzhou, 225001, Jiangsu Province, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Pan
authors.affiliation: Department of Cardiothoracic Surgery, College of Clinical Medicine, Yangzhou University, Yangzhou, 225001, Jiangsu Province, China.|Yangzhou University Medical College, Yangzhou, 225001, Jiangsu Province, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Ye
authors.affiliation: Department of Cardiothoracic Surgery, College of Clinical Medicine, Yangzhou University, Yangzhou, 225001, Jiangsu Province, China.|Yangzhou University Medical College, Yangzhou, 225001, Jiangsu Province, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Cardiothoracic Surgery, College of Clinical Medicine, Yangzhou University, Yangzhou, 225001, Jiangsu Province, China.|Yangzhou University Medical College, Yangzhou, 225001, Jiangsu Province, China.
authors.forename: Fangbiao
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Cardiothoracic Surgery, College of Clinical Medicine, Yangzhou University, Yangzhou, 225001, Jiangsu Province, China.|Yangzhou University Medical College, Yangzhou, 225001, Jiangsu Province, China.|Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou, 225001, Jiangsu Province, China.
authors.forename: Chonghao
authors.initials: C
authors.lastname: Zhong
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001696:Biomechanical Phenomena; D002642:Chick Embryo; D005109:Extracellular Matrix / Q000737:chemistry; D064593:Heterografts; D039823:Iridoids / Q000737:chemistry; D008297:Male; D008422:Materials Testing; D018919:Neovascularization, Physiologic; D011817:Rabbits; D051381:Rats; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014132:Trachea / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D039823:Iridoids; C007834:genipin