title: The South Pacific epidemic strain of Zika virus replicates efficiently in human epithelial A549 cells leading to IFN-β production and apoptosis induction.
abstract: Zika virus (ZIKV) is an emerging flavivirus since the first epidemics in South Pacific in 2007. The recent finding that ZIKV is now circulating in Western Hemisphere and can be associated to severe human diseases, warrants the need for its study. Here we evaluate the susceptibility of human lung epithelial A549 cells to South Pacific epidemic strain of ZIKV isolated in 2013. We showed that ZIKV growth in A549 cells is greatly efficient. ZIKV infection resulted in the secretion of IFN-β followed by the expression of pro-inflammatory cytokines such as IL-1β, and transcriptional activity of IFIT genes. At the maximum of virus progeny production, ZIKV triggers mitochondrial apoptosis through activation of caspases-3 and -9. Whereas at early infection times, the rapid release of IFN-β which exerts an antiviral effect against ZIKV might delay apoptosis in infected cells.
authors.affiliation: Université de la Réunion, UM 134 Processus Infectieux en Milieu Insulaire Tropical (PIMIT), INSERM U1187, CNRS UMR9192, IRD UMR249. Plateforme Technologique CYROI, 97490 Sainte Clotilde, France.
authors.forename: Etienne
authors.initials: E
authors.lastname: Frumence
authors.affiliation: Université de la Réunion, UM 134 Processus Infectieux en Milieu Insulaire Tropical (PIMIT), INSERM U1187, CNRS UMR9192, IRD UMR249. Plateforme Technologique CYROI, 97490 Sainte Clotilde, France.
authors.forename: Marjolaine
authors.initials: M
authors.lastname: Roche
authors.affiliation: Université de la Réunion, UM 134 Processus Infectieux en Milieu Insulaire Tropical (PIMIT), INSERM U1187, CNRS UMR9192, IRD UMR249. Plateforme Technologique CYROI, 97490 Sainte Clotilde, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Krejbich-Trotot
authors.affiliation: Université de la Réunion, UM 134 Processus Infectieux en Milieu Insulaire Tropical (PIMIT), INSERM U1187, CNRS UMR9192, IRD UMR249. Plateforme Technologique CYROI, 97490 Sainte Clotilde, France.
authors.forename: Chaker
authors.initials: C
authors.lastname: El-Kalamouni
authors.affiliation: Université de la Réunion, UMR Diabète Athérothrombose Thérapies Réunion Océan Indien (DeTROI), INSERM U1188, Plateforme Technologique CYROI, 97490 Sainte Clotilde, France.
authors.forename: Brice
authors.initials: B
authors.lastname: Nativel
authors.affiliation: Université de la Réunion, UMR Diabète Athérothrombose Thérapies Réunion Océan Indien (DeTROI), INSERM U1188, Plateforme Technologique CYROI, 97490 Sainte Clotilde, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Rondeau
authors.affiliation: Laboratoire MIVEGEC, UMR 224 IRD/CNRS/UM, 34394 Montpellier, France.
authors.forename: Dorothée
authors.initials: D
authors.lastname: Missé
authors.affiliation: Université de la Réunion, UM 134 Processus Infectieux en Milieu Insulaire Tropical (PIMIT), INSERM U1187, CNRS UMR9192, IRD UMR249. Plateforme Technologique CYROI, 97490 Sainte Clotilde, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Gadea
authors.affiliation: Université de la Réunion, UM 134 Processus Infectieux en Milieu Insulaire Tropical (PIMIT), INSERM U1187, CNRS UMR9192, IRD UMR249. Plateforme Technologique CYROI, 97490 Sainte Clotilde, France. Electronic address: wildriss.viranaicken@univ-reunion.fr.
authors.forename: Wildriss
authors.initials: W
authors.lastname: Viranaicken
authors.affiliation: Université de la Réunion, UM 134 Processus Infectieux en Milieu Insulaire Tropical (PIMIT), INSERM U1187, CNRS UMR9192, IRD UMR249. Plateforme Technologique CYROI, 97490 Sainte Clotilde, France. Electronic address: philippe.despres@univ-reunion.fr.
authors.forename: Philippe
authors.initials: P
authors.lastname: Desprès
mesh_terms: D000072283:A549 Cells; D056809:Alveolar Epithelial Cells / Q000378:metabolism / Q000821:virology*; D000818:Animals; D017209:Apoptosis; D001343:Autophagy; D002522:Chlorocebus aethiops; D016207:Cytokines / Q000096:biosynthesis; D006801:Humans; D016899:Interferon-beta / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D008928:Mitochondria; D014709:Vero Cells; D014779:Virus Replication; D000071244:Zika Virus / Q000502:physiology*
chemical_list: D016207:Cytokines; D016899:Interferon-beta
keywords: Apoptosis; Arbovirus; Emerging virus; Human epithelial cells; Innate immunity; Oxidative stress; Type-I interferon; Viral pathogenicity; Zika virus