title: A Fluorescence Approach to Assess the Production of Soluble Microbial Products from Aerobic Granular Sludge Under the Stress of 2,4-Dichlorophenol.
abstract: In this study, a fluorescence approach was used to evaluate the production of soluble microbial products (SMP) in aerobic granular sludge system under the stress of 2,4-dichlorophenol (2,4-DCP). A combined use of three-dimension excitation emission matrix fluorescence spectroscopy (3D-EEM), Parallel factor analysis (PARAFAC), synchronous fluorescence and two-dimensional correlation spectroscopy (2D-COS) were explored to respect the SMP formation in the exposure of different doses of 2,4-DCP. Data implied that the presence of 2,4-DCP had an obvious inhibition on biological nitrogen removal. According to EEM-PARAFAC, two fluorescent components were derived and represented to the presence of fulvic-like substances and humic-like substances in Component 1 and protein-like substances in Component 2. It was found from synchronous fluorescence that protein-like peak presented slightly higher intensity than that of fulvic-like peak. 2D-COS further revealed that fluorescence change took place sequentially in the following order: protein-like fraction > fulvic-like fraction. The obtained results could provide a potential application of fluorescence spectra in the released SMP assessment in the exposure of toxic compound during wastewater treatment.
authors.affiliation: School of Resources and Environment, University of Jinan, Jinan 250022, PR China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wei
authors.affiliation: School of Resources and Environment, University of Jinan, Jinan 250022, PR China.
authors.forename: Heng
authors.initials: H
authors.lastname: Dong
authors.affiliation: School of Resources and Environment, University of Jinan, Jinan 250022, PR China.
authors.forename: Na
authors.initials: N
authors.lastname: Wu
authors.affiliation: School of Civil and Environmental Engineering, University of Technology Sydney, Broadway, NSW 2007, Australia.
authors.forename: Huu Hao
authors.initials: HH
authors.lastname: Ngo
authors.affiliation: School of Civil and Environmental Engineering, University of Technology Sydney, Broadway, NSW 2007, Australia.
authors.forename: Wenshan
authors.initials: W
authors.lastname: Guo
authors.affiliation: School of Resources and Environment, University of Jinan, Jinan 250022, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Du
authors.affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
mesh_terms: D000332:Aerobiosis; D001688:Biological Products / Q000032:analysis*; D002733:Chlorophenols / Q000633:toxicity*; D059013:Microbial Consortia / Q000187:drug effects*; D009584:Nitrogen / Q000378:metabolism; D012722:Sewage / Q000382:microbiology*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D001688:Biological Products; D002733:Chlorophenols; D012722:Sewage; D014874:Water Pollutants, Chemical; D009584:Nitrogen; C004762:2,4-dichlorophenol