title: Development and Characterization of the Paclitaxel loaded Riboflavin and Thiamine Conjugated Carbon Nanotubes for Cancer Treatment.
abstract: OBJECTIVE
  In the present investigation, we prepared and evaluated the paclitaxel loaded riboflavin and thiamine conjugated multi walled carbon nanotubes (PTX-Rf-MWCNTs and PTX-Tm-MWCNTs) for targeted delivery to cancer employing MCF-7 cancer cell lines.
  
  
  METHODS
  The developed conjugates were characterized using FTIR, NMR spectroscopy, electron microscopy drug loading, release, stability, hemolytic, ex vivo and in vivo studies etc.
  
  
  RESULTS
  The percent entrapment efficiency was found to be 87.92 ± 0.48 and 82.75 ± 0.47% of PTX-Tm-MWCNTs, PTX-Rf-MWCNTs, respectively. The percent hemolysis of purified MWCNTs, PTX-MWCNTs, PTX-Tm-MWCNTs and PTX-Rf-MWCNTs was found to be 20.49 ± 0.97, 37.39 ± 0.78, 14.61 ± 0.84 and 11.17 ± 0.77% respectively. The PTX-Tm-MWCNTs and PTX-Rf-MWCNTs showed more cytotoxic effect as compared to PTX and PTX-MWCNTs with PTX-Rf-MWCNTs exhibiting the maximum cytotoxic potential.
  
  
  CONCLUSIONS
  Thus in final outcome, we concluded that the riboflavin and thiamine conjugated MWCNTs shown great promising potential in the treatment of cancer, but more exhaustive data is needed in future.
authors.affiliation: Pharmaceutics Research Laboratory, Department of Pharmaceutical Sciences, Dr. H. S. Gour Central University, Sagar, 470 003, India.
authors.forename: Swati
authors.initials: S
authors.lastname: Singh
authors.affiliation: Pharmaceutics Research Laboratory, Department of Pharmaceutical Sciences, Dr. H. S. Gour Central University, Sagar, 470 003, India. neelesh81mph@gmail.com.|School of Pharmaceutical Sciences, Rajiv Gandhi Technical University, Airport Bypass Road, Gandhi Nagar, Bhopal, M.P., 462 036, India. neelesh81mph@gmail.com.|Department of Pharmaceutical Sciences, Irma Lerma Rangel College of Pharmacy, Texas A & M Health Science Centre, Kingsville, Texas, 78363, USA. neelesh81mph@gmail.com.
authors.forename: Neelesh Kumar
authors.initials: NK
authors.lastname: Mehra
authors.affiliation: Pharmaceutics Research Laboratory, Department of Pharmaceutical Sciences, Dr. H. S. Gour Central University, Sagar, 470 003, India.|School of Pharmaceutical Sciences, Rajiv Gandhi Technical University, Airport Bypass Road, Gandhi Nagar, Bhopal, M.P., 462 036, India.
authors.forename: N K
authors.initials: NK
authors.lastname: Jain
mesh_terms: D045744:Cell Line, Tumor; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D006801:Humans; D061986:MCF-7 Cells; D037742:Nanotubes, Carbon / Q000737:chemistry*; D017239:Paclitaxel / Q000737:chemistry* / Q000494:pharmacology*; D012256:Riboflavin / Q000737:chemistry* / Q000494:pharmacology*; D013831:Thiamine / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D004337:Drug Carriers; D037742:Nanotubes, Carbon; D017239:Paclitaxel; D012256:Riboflavin; D013831:Thiamine
keywords: MCF-7 breast cancer cell line; carbon nanotubes; paclitaxel; riboflavin; thiamine; vitamins