title: Shell matrix proteins of the clam, Mya truncata: Roles beyond shell formation through proteomic study.
abstract: Mya truncata, a soft shell clam, is presented as a new model to study biomineralization through a proteomics approach. In this study, the shell and mantle tissue were analysed in order to retrieve knowledge about the secretion of shell matrix proteins (SMPs). Out of 67 and 127 shell and mantle proteins respectively, 16 were found in both shell and mantle. Bioinformatic analysis of SMP sequences for domain prediction revealed the presence of several new domains such as fucolectin tachylectin-4 pentraxin-1 (FTP), scavenger receptor, alpha-2-macroglobulin (α2 M), lipocalin and myosin tail along with previously reported SMP domains such as chitinase, carbonic anhydrase, tyrosinase, sushi, and chitin binding. Interestingly, these newly predicted domains are attributed with molecular functions other than biomineralization. These findings suggest that shells may not only act as protective armour from predatory action, but could also actively be related to other functions such as immunity. In this context, the roles of SMPs in biomineralization need to be looked in a new perspective.
authors.affiliation: UMR 7245 CNRS/MNHN Molécules de Communications et Adaptations des Micro-organismes, Sorbonne Universités, Muséum National d'Histoire Naturelle, Paris, France; UMR 7208 CNRS/MNHN/UPMC/IRD Biologie des Organismes Aquatiques et Ecosystèmes, Sorbonne Universités, Muséum National d'Histoire Naturelle, Paris, France.
authors.forename: Jaison
authors.initials: J
authors.lastname: Arivalagan
authors.affiliation: UMR 7245 CNRS/MNHN Molécules de Communications et Adaptations des Micro-organismes, Sorbonne Universités, Muséum National d'Histoire Naturelle, Paris, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Marie
authors.affiliation: British Antarctic Survey, Natural Environment Research Council, High Cross, Madingley Road, Cambridge, CB3 0ET, UK.
authors.forename: Victoria A
authors.initials: VA
authors.lastname: Sleight
authors.affiliation: British Antarctic Survey, Natural Environment Research Council, High Cross, Madingley Road, Cambridge, CB3 0ET, UK.
authors.forename: Melody S
authors.initials: MS
authors.lastname: Clark
authors.affiliation: UMR 7208 CNRS/MNHN/UPMC/IRD Biologie des Organismes Aquatiques et Ecosystèmes, Sorbonne Universités, Muséum National d'Histoire Naturelle, Paris, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Berland
authors.affiliation: UMR 7245 CNRS/MNHN Molécules de Communications et Adaptations des Micro-organismes, Sorbonne Universités, Muséum National d'Histoire Naturelle, Paris, France.
authors.forename: Arul
authors.initials: A
authors.lastname: Marie
mesh_terms: D060105:Animal Shells / Q000254:growth & development* / Q000378:metabolism; D000818:Animals; D002113:Calcification, Physiologic; D049892:Mya / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D020543:Proteome; D040901:Proteomics; D012606:Scotland
chemical_list: D020543:Proteome
keywords: Biomineralization; Mya truncata; Shell matrix proteins