title: Isolation of Mycobacterium kumamotonense from a patient with pulmonary infection and latent tuberculosis.
abstract: Mycobacterium kumamotonense is a novel, slow-growing non-chromogenic nontuberculous mycobacterium, which belongs to Mycobacterium terrae complex. We report, for the first time in Greece, the isolation of M. kumamotonense from an immunocompetent patient with pulmonary infection and latent tuberculosis. M. kumamotonense was identified by sequencing analysis of 16S rDNA and 65-kDa heat shock protein genes while by commercial molecular assays it was misidentified as Mycobacterium celatum. Antibiotic susceptibility testing was performed by the reference broth microdilution method. The strain was susceptible to amikacin, clarithromycin, rifampin, ciprofloxacin, moxifloxacin, rifabutin, ethambutol and linezolid.
authors.affiliation: Department of the Clinical Microbiology Laboratory, Medical School of Athens, Attikon University Hospital, Athens, Greece.
authors.forename: Fanourios
authors.initials: F
authors.lastname: Kontos
authors.forename: Dimitrios Nikitas
authors.initials: DN
authors.lastname: Mavromanolakis
authors.forename: Marina Chari
authors.initials: MC
authors.lastname: Zande
authors.forename: Zoe Georgios
authors.initials: ZG
authors.lastname: Gitti
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins; D018834:Chaperonin 60; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005260:Female; D006115:Greece; D006801:Humans; D055985:Latent Tuberculosis / Q000150:complications*; D008826:Microbial Sensitivity Tests; D009165:Mycobacterium Infections, Nontuberculous / Q000175:diagnosis* / Q000382:microbiology*; D009170:Nontuberculous Mycobacteria / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification*; D010802:Phylogeny; D018410:Pneumonia, Bacterial / Q000175:diagnosis* / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; C074119:heat-shock protein 65, Mycobacterium