title: Dissulfurirhabdus thermomarina gen. nov., sp. nov., a thermophilic, autotrophic, sulfite-reducing and disproportionating deltaproteobacterium isolated from a shallow-sea hydrothermal vent.
abstract: A thermophilic, anaerobic, chemolithoautotrophic bacterium, strain SH388T, was isolated from a shallow, submarine hydrothermal vent (Kuril Islands, Russia). Cells of strain SH388Twere Gram-stain-negative short rods, 0.2-0.4 µm in diameter and 1.0-2.5 µm in length, and motile with flagella. The temperature range for growth was 25-58 °C (optimum 50 °C), and the pH range for growth was pH 5.0-7.0 (optimum pH 6.0-6.5). Growth of strain SH388Twas observed in the presence of NaCl concentrations ranging from 0.5 to 4.0 % (w/v) (optimum 2.0-2.5 %). The strain grew chemolithoautotrophically with molecular hydrogen as electron donor, sodium sulfite as electron acceptor and bicarbonate/CO2 as a carbon source. It was also able to grow by disproportionation of sulfite and elemental sulfur but not thiosulfate. Sulfate, Fe(III) and nitrate were not used as electron acceptors either with H2 or organic electron donors. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belonged to the class Deltaproteobacteria and was most closely related to Dissulfuribacter thermophilus and Dissulfurimicrobium hydrothermale (91.6 % and 90.4 % sequence similarity). On the basis of its physiological properties and results of phylogenetic analyses, strain SH388Tis considered to represent a novel species of a new genus, for which the name Dissulfurirhabdus thermomarina gen. nov., sp. nov. is proposed. The type strain of the species is SH388T(=DSM 100025T=VKM B-2960T). It is the first thermophilic disproportionator of sulfur compounds isolated from a shallow-sea environment.
authors.affiliation: Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Leninskiy Prospect 33, bld. 2, 119071 Moscow, Russia.
authors.forename: Galina B
authors.initials: GB
authors.lastname: Slobodkina
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Leninskiy 33, bld. 2, 119071 Moscow, Russia.
authors.forename: Tatyana V
authors.initials: TV
authors.lastname: Kolganova
authors.affiliation: Gubkin Russian State University of Oil and Gas, Leninsskiy Prospect 65, 117485, Moscow, Russia.
authors.forename: Dmitry S
authors.initials: DS
authors.lastname: Kopitsyn
authors.affiliation: Chemistry Department, Lomonosov Moscow State University, Leninskie Gory 1, 119899 Moscow, Russia.
authors.forename: Mikhail B
authors.initials: MB
authors.lastname: Viryasov
authors.affiliation: Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Leninskiy Prospect 33, bld. 2, 119071 Moscow, Russia.
authors.forename: Elizaveta A
authors.initials: EA
authors.lastname: Bonch-Osmolovskaya
authors.affiliation: Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Leninskiy Prospect 33, bld. 2, 119071 Moscow, Russia.
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Slobodkin
mesh_terms: D052818:Autotrophic Processes; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D020564:Deltaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005227:Fatty Acids / Q000737:chemistry; D060086:Hydrothermal Vents / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012426:Russia; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013447:Sulfites / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D013447:Sulfites; D013455:Sulfur
keywords: Deltaproteobacteria; anaerobe; chemolithoautotroph; disproportionation of sulfur compounds; sulfite reduction; thermophile