title: Drug loaded composite oxidized pectin and gelatin networks for accelerated wound healing.
abstract: Biocomposite interactive wound dressings have been designed and fabricated using oxidized pectin (OP), gelatin and nonwoven cotton fabric. Due to their inherent virtues of antimicrobial activity and cytocompatibility, these composite structures are capable of redirecting the healing cascade and influencing cell attachment and proliferation. A novel in situ reduction process has been followed to synthesize oxidized pectin-gelatin-nanosilver (OP-Gel-NS) flower like nanohydrocolloids. This encapsulation technology controls the diffusion and permeation of nanosilver into the surrounding biological tissues. Ciprofloxacin hydrochloride has also been incorporated into the OP-Gel matrix to produce OP-Gel-Cipro dressings. While OP-Gel-NS dressings exhibited 100% antimicrobial activity at extremely low loadings of 3.75μg/cm(2), OP-Gel-Cipro dressings were highly antimicrobial at 1% drug loading. While NIH3T3 mouse fibroblasts proliferated remarkably well when cultured with OP-Gel and OP-Gel-Cipro dressings, OP-Gel-NS hindered cell growth and Bactigras(®) induced complete lysis. Full thickness excisional wounds were created on C57BL/6J mice and the wound healing potential of the OP-Gel-NS dressings led to accelerated healing within 12days, while OP-Gel-Cipro dressings healed wounds at a rate similar to that of Bactigras(®). Histological examination revealed that OP-Gel-NS and OP-Gel-Cipro treatment led to organized collagen deposition, neovascularization and nuclei migration, unlike Bactigras(®). Therefore, the OP-Gel-NS and OP-Gel-Cipro biocomposite dressings exhibiting good hydrophilicity, sustained antimicrobial nature, promote cell growth and proliferation, and lead to rapid healing, can be considered viable candidates for effective management.
authors.affiliation: Bioengineering Lab, Department of Textile Technology, Indian Institute of Technology, New Delhi 16, India.
authors.forename: Mythili
authors.initials: M
authors.lastname: Tummalapalli
authors.affiliation: Laboratory of Tissue Biology and Therapeutic Engineering, IBCP, UMR 5305 CNRS-UCBL-Lyon Cedex 07, France.
authors.forename: Morgane
authors.initials: M
authors.lastname: Berthet
authors.affiliation: Laboratory of Tissue Biology and Therapeutic Engineering, IBCP, UMR 5305 CNRS-UCBL-Lyon Cedex 07, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Verrier
authors.affiliation: Bioengineering Lab, Department of Textile Technology, Indian Institute of Technology, New Delhi 16, India.
authors.forename: B L
authors.initials: BL
authors.lastname: Deopura
authors.affiliation: Department of Chemistry, Jamia Hamdard, New Delhi 62, India.
authors.forename: M S
authors.initials: MS
authors.lastname: Alam
authors.affiliation: Bioengineering Lab, Department of Textile Technology, Indian Institute of Technology, New Delhi 16, India. Electronic address: bgupta@textile.iitd.ernet.in.
authors.forename: Bhuvanesh
authors.initials: B
authors.lastname: Gupta
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D001458:Bandages; D049109:Cell Proliferation / Q000187:drug effects; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002939:Ciprofloxacin / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D003094:Collagen / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005780:Gelatin / Q000737:chemistry*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D041681:NIH 3T3 Cells; D053758:Nanoparticles; D010084:Oxidation-Reduction; D010368:Pectins / Q000737:chemistry*; D012835:Silver Nitrate / Q000737:chemistry; D013997:Time Factors; D014018:Tissue Distribution; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000890:Anti-Infective Agents; D002939:Ciprofloxacin; D010368:Pectins; D005780:Gelatin; D003094:Collagen; D012835:Silver Nitrate
keywords: Biocomposite; Cell compatibility; Gelatin; Infection control; Oxidized pectin; Wound healing