title: Epithelial Cell-Derived a Disintegrin and Metalloproteinase-17 Confers Resistance to Colonic Inflammation Through EGFR Activation.
abstract: Epithelial regeneration is a key process for the recovery from ulcerative colitis (UC). Here we demonstrate that a disintegrin and metalloproteinase-17 (ADAM17), a main sheddase for tumor necrosis factor (TNF)-α, is essential for defensive epithelial properties against UC by promoting epithelial cell growth and goblet cell differentiation in mouse and human. Mice with systemic deletion of Adam17 developed severe dextran sulfate sodium-induced colitis when compared to mice with myeloid cell Adam17 deletion or control littermates. ADAM17 was predominantly expressed by regenerating epithelia in control mice, and its loss or inhibition attenuated epidermal growth factor receptor (EGFR) activation, epithelial proliferation, mucus production and barrier functions. Conversely, ectopic EGFR stimulation promoted epithelial regeneration thereby partially rescuing the severe colitis caused by ADAM17 deficiency. In UC patients, epithelial ADAM17 expression positively correlated with both cell proliferation and goblet cell number. These findings suggest that maintaining ADAM17-EGFR epithelial signaling is necessary for the recovery from UC and would be beneficial to therapeutic strategies targeting ADAM17-mediated TNF-α shedding.
authors.affiliation: Department of Pathology, Keio University School of Medicine, Tokyo, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shimoda
authors.affiliation: Department of Orthopedic Surgery, Keio University School of Medicine, Tokyo, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Horiuchi
authors.affiliation: Department of Pathology, Keio University School of Medicine, Tokyo, Japan.
authors.forename: Aya
authors.initials: A
authors.lastname: Sasaki
authors.affiliation: Department of Diagnostic Pathology, Fujita Health University School of Medicine, Aichi, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Tsukamoto
authors.affiliation: Department of Surgery, Keio University School of Medicine, Tokyo, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Okabayashi
authors.affiliation: Department of Surgery, Keio University School of Medicine, Tokyo, Japan.
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Hasegawa
authors.affiliation: Department of Surgery, Keio University School of Medicine, Tokyo, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kitagawa
authors.affiliation: Department of Pathology, Keio University School of Medicine, Tokyo, Japan; Department of Pathophysiology for Locomotive and Neoplastic Diseases, Juntendo University, Graduate School of Medicine, Tokyo, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Okada
mesh_terms: D000072198:ADAM17 Protein / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D049109:Cell Proliferation / Q000235:genetics; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D003106:Colon / Q000378:metabolism / Q000473:pathology; D016264:Dextran Sulfate / Q000633:toxicity; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D066246:ErbB Receptors / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation; D020397:Goblet Cells / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007249:Inflammation / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D015398:Signal Transduction; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D014409:Tumor Necrosis Factor-alpha; D016264:Dextran Sulfate; C541485:EGFR protein, mouse; D066246:ErbB Receptors; D000072198:ADAM17 Protein; C000606463:Adam17 protein, mouse
keywords: A disintegrin and metalloproteinase 17 (ADAM17); ADAM, a disintegrin and metalloproteinase; BrdU, bromodeoxyuridine; DSS, dextran sulfate sodium; EGF, epidermal growth factor; EGFR, epidermal growth factor receptor; Epidermal growth factor receptor (EGFR); Epithelial barrier; Goblet cell; IBD, inflammatory bowel disease; MAPK, mitogen activated protein kinase; MMP, matrix metalloproteinase; PCNA, proliferation cell nuclear antigen; PI3K, phosphatidylinositol 3-kinase; RT-qPCR, real-time quantitative PCR; STAT3, signal transducer and activator of transcription 3; TACE, tumor necrosis factor-α converting enzyme; TGF, transforming growth factor; TGM, transglutaminase; TNF, tumor necrosis factor; UC, ulcerative colitis; Ulcerative colitis; pEGFR, phosphorylated EGFR; pIpC, polyinosinic–polycytidylic acid