title: Characterization of tet(Y)-carrying LowGC plasmids exogenously captured from cow manure at a conventional dairy farm.
abstract: Manure from dairy farms has been shown to contain diverse tetracycline resistance genes that are transferable to soil. Here, we focus on conjugative plasmids that may spread tetracycline resistance at a conventional dairy farm. We performed exogenous plasmid isolation from cattle feces using chlortetracycline for transconjugant selection. The transconjugants obtained harbored LowGC-type plasmids and tet(Y). A representative plasmid (pFK2-7) was fully sequenced and this was compared with previously described LowGC plasmids from piggery manure-treated soil and a GenBank record from Acinetobacter nosocomialis that we also identified as a LowGC plasmid. The pFK2-7 plasmid had the conservative backbone typical of LowGC plasmids, though this region was interrupted with an insert containing the tet(Y)-tet(R) tetracycline resistance genes and the strA-strB streptomycin resistance genes. Despite Acinetobacter populations being considered natural hosts of LowGC plasmids, these plasmids were not found in three Acinetobacter isolates from the study farm. The isolates harbored tet(Y)-tet(R) genes in identical genetic surroundings as pFK2-7, however, suggesting genetic exchange between Acinetobacter and LowGC plasmids. Abundance of LowGC plasmids and tet(Y) was correlated in manure and soil samples from the farm, indicating that LowGC plasmids may be involved in the spread of tet(Y) in the environment.
authors.affiliation: Biology Centre of the Czech Academy of Sciences, Institute of Soil Biology, Na Sádkách 7, 370 05 České Budějovice, Czech Republic.
authors.forename: Martina
authors.initials: M
authors.lastname: Kyselková
authors.affiliation: Biology Centre of the Czech Academy of Sciences, Institute of Soil Biology, Na Sádkách 7, 370 05 České Budějovice, Czech Republic chrudimsky@upb.cas.cz.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Chrudimský
authors.affiliation: Biology Centre of the Czech Academy of Sciences, Institute of Parasitology, Branišovská 31, 370 05 České Budějovice, Czech Republic Faculty of Science, University of South Bohemia, Branišovská 31, 370 05 České Budějovice, Czech Republic.
authors.forename: Filip
authors.initials: F
authors.lastname: Husník
authors.affiliation: Biology Centre of the Czech Academy of Sciences, Institute of Soil Biology, Na Sádkách 7, 370 05 České Budějovice, Czech Republic.
authors.forename: Alica
authors.initials: A
authors.lastname: Chroňáková
authors.affiliation: Department of Epidemiology and Pathogen Diagnostics, Julius Kühn-Institut, Federal Research Centre for Cultivated Plants (JKI), Messeweg 11/12, 38104 Braunschweig, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Heuer
authors.affiliation: Department of Epidemiology and Pathogen Diagnostics, Julius Kühn-Institut, Federal Research Centre for Cultivated Plants (JKI), Messeweg 11/12, 38104 Braunschweig, Germany.
authors.forename: Kornelia
authors.initials: K
authors.lastname: Smalla
authors.affiliation: Biology Centre of the Czech Academy of Sciences, Institute of Soil Biology, Na Sádkách 7, 370 05 České Budějovice, Czech Republic.
authors.forename: Dana
authors.initials: D
authors.lastname: Elhottová
mesh_terms: D000150:Acinetobacter / Q000187:drug effects / Q000235:genetics*; D000383:Agriculture; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001482:Base Composition / Q000235:genetics*; D001483:Base Sequence / Q000235:genetics; D002417:Cattle; D002751:Chlortetracycline / Q000494:pharmacology*; D002874:Chromosome Mapping; D000072480:Farms; D005260:Female; D008372:Manure / Q000032:analysis*; D010957:Plasmids / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012987:Soil; D012988:Soil Microbiology; D013307:Streptomycin / Q000494:pharmacology*; D013552:Swine; D013753:Tetracycline Resistance / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D008372:Manure; D012987:Soil; D002751:Chlortetracycline; D013307:Streptomycin
keywords: Acinetobacter; LowGC plasmids; cattle manure; horizontal gene transfer; tet(Y); tetracycline resistance