title: Complete genome sequence of Vibrio parahaemolyticus FORC_023 isolated from raw fish storage water.
abstract: Vibrio parahaemolyticusis a Gram-negative halophilic bacterium that causes food-borne gastroenteritis in humans who consumeV. parahaemolyticus-contaminated seafood.The FORC_023 strain was isolated from raw fish storage water, containing live fish at a sashimi restaurant. Here, we aimed to sequence and characterize the genome of the FORC_023 strain. The genome of the FORC_023 strain showed two circular chromosomes, which contained 4227 open reading frames (ORFs), 131 tRNA genes and 37 rRNA genes. Although the genome of FORC_023 did not include major virulence genes, such as genes encoding thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH), it contained genes encoding other hemolysins, secretion systems, iron uptake-related proteins and severalV. parahaemolyticusislands. The highest average nucleotide identity value was obtained between the FORC_023 strain and UCM-V493 (CP007004-6). Comparative genomic analysis of FORC_023 with UCM-V493 revealed that FORC_023 carried an additional genomic region encoding virulence factors, such as repeats-in-toxin and type II secretion factors. Furthermore,in vitrocytotoxicity testing showed that FORC_023 exhibited a high level of cytotoxicity toward INT-407 human epithelial cells. These results suggested that the FORC_023 strain may be a food-borne pathogen.
authors.affiliation: Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Han Young
authors.initials: HY
authors.lastname: Chung
authors.affiliation: Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Eun Jung
authors.initials: EJ
authors.lastname: Na
authors.affiliation: Department of Life Science, Sogang University, 35 Baekbeom-Ro, Mapo-Gu, Seoul 121-742, Republic of Korea.
authors.forename: Kyu-Ho
authors.initials: KH
authors.lastname: Lee
authors.affiliation: Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Sangryeol
authors.initials: S
authors.lastname: Ryu
authors.affiliation: Department of Applied Chemistry & Biological Engineering, Ajou University, Yeongtong-gu, Suwon 443-749, Republic of Korea.
authors.forename: Hyunjin
authors.initials: H
authors.lastname: Yoon
authors.affiliation: Department of Food Science and Biotechnology, Kyung Hee University, 1732 Deokyoungdae-ro, Yongin 17104, Republic of Korea.
authors.forename: Ju-Hoon
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Animal Resources Science, Dankook University, 119 Dandae-ro, Cheonan 330-714, Republic of Korea.
authors.forename: Hyeun Bum
authors.initials: HB
authors.lastname: Kim
authors.affiliation: Department of Animal Science & Biotechnology, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Heebal
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Choi
authors.affiliation: Department of Life Science, Hallym University, 1 Hallymdaehak-gil, Chuncheon 200-702, Republic of Korea bkim79@hallym.ac.kr.
authors.forename: Bong-Soo
authors.initials: BS
authors.lastname: Kim
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D019295:Computational Biology / Q000379:methods; D005399:Fishes / Q000382:microbiology*; D005516:Food Microbiology; D023061:Gene Order; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods; D006801:Humans; D017422:Sequence Analysis, DNA; D014735:Vibrio Infections / Q000382:microbiology; D014736:Vibrio parahaemolyticus / Q000235:genetics* / Q000302:isolation & purification; D037521:Virulence Factors / Q000235:genetics
chemical_list: D037521:Virulence Factors
keywords: V. parahaemolyticus island; Vibrio parahaemolyticus; genome sequence; comparative analysis; food-borne pathogen; virulence factor