title: Bacterial populations on brewery filling hall surfaces as revealed by next-generation sequencing.
abstract: Due to the presence of moisture and nutrients, brewery filling line surfaces are susceptible to unwanted microbial attachment. Knowledge of the attaching microbes will aid in designing hygienic control of the process. In this study the bacterial diversity present on brewery filling line surfaces was revealed by next generation sequencing. The two filling lines studied maintained their characteristic bacterial community throughout three sampling times (13-163 days). On the glass bottle line, γ-proteobacteria dominated (35-82% of all OTUs), whereas on the canning line α-, β- and γ-proteobacteria and actinobacteria were most common. The most frequently detected genera were Acinetobacter, Propinobacterium and Pseudomonas. The halophilic genus Halomonas was commonly detected, which might be due to its tolerance to alkaline foam cleaners. This study has revealed a detailed overall picture of the bacterial groups present on filling line surfaces. Further effort should be given to determine the efficacy of washing procedures on different bacterial groups.
authors.affiliation: a VTT Technical Research Centre of Finland, Solutions for Natural Resources and the Environment , Espoo , Finland.
authors.forename: Outi
authors.initials: O
authors.lastname: Priha
authors.affiliation: a VTT Technical Research Centre of Finland, Solutions for Natural Resources and the Environment , Espoo , Finland.
authors.forename: Mari
authors.initials: M
authors.lastname: Raulio
authors.affiliation: a VTT Technical Research Centre of Finland, Solutions for Natural Resources and the Environment , Espoo , Finland.
authors.forename: Johanna
authors.initials: J
authors.lastname: Maukonen
authors.affiliation: b Hartwall , Lahti , Finland.
authors.forename: Anna-Kaisa
authors.initials: AK
authors.lastname: Vehviläinen
authors.affiliation: a VTT Technical Research Centre of Finland, Solutions for Natural Resources and the Environment , Espoo , Finland.
authors.forename: Erna
authors.initials: E
authors.lastname: Storgårds
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001628:Beverages / Q000382:microbiology*; D005511:Food Handling; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Illumina MiSeq; NGS; Process hygiene; bacterial community; filling line; next generation sequencing