title: The structure of the lipooligosaccharide from Xanthomonas oryzae pv. Oryzae: the causal agent of the bacterial leaf blight in rice.
abstract: The structure of the lipooligosaccharide (LOS) from the rice pathogen Xanthomonas oryzae pv. oryzae has been elucidated. The characterization of the core oligosaccharide structure was obtained by the employment of two chemical degradation protocols and by analysis of the products via NMR spectroscopy. The structure of the lipid A portion was achieved by MALDI mass spectrometry analysis on purified lipid A. The LOS from Xanthomonas oryzae pv. oryzae revealed to possess the same core structure of Xanthomonas campestris pv. campestris and interesting novel features on its lipid A domain. The evaluation of the biological activity of both LOS and isolated lipid A was also executed.
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, via Cinthia 4, 80126 Naples, Italy. Electronic address: flaviana.dilorenzo@unina.it.
authors.forename: Flaviana
authors.initials: F
authors.lastname: Di Lorenzo
authors.affiliation: CNR-Istituto per i Polimeri, Compositi e Biomateriali IPCB Unità di Catania, Via Gaifami 18, 95126 Catania, Italy.
authors.forename: Angelo
authors.initials: A
authors.lastname: Palmigiano
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, via Cinthia 4, 80126 Naples, Italy.
authors.forename: Alba
authors.initials: A
authors.lastname: Silipo
authors.affiliation: Department of Life Sciences, School of Agriculture, Meiji University, 1-1-1 Higashi-Mita, Tama-ku, Kawasaki 214-8571, Japan.
authors.forename: Yoshitake
authors.initials: Y
authors.lastname: Desaki
authors.affiliation: CNR-Istituto per i Polimeri, Compositi e Biomateriali IPCB Unità di Catania, Via Gaifami 18, 95126 Catania, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Garozzo
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, via Cinthia 4, 80126 Naples, Italy.
authors.forename: Rosa
authors.initials: R
authors.lastname: Lanzetta
authors.affiliation: Department of Life Sciences, School of Agriculture, Meiji University, 1-1-1 Higashi-Mita, Tama-ku, Kawasaki 214-8571, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Shibuya
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, via Cinthia 4, 80126 Naples, Italy. Electronic address: molinaro@unina.it.
authors.forename: Antonio
authors.initials: A
authors.lastname: Molinaro
mesh_terms: D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D012275:Oryza / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014974:Xanthomonas / Q000737:chemistry / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; C023023:lipid-linked oligosaccharides
keywords: Bacterial leaf blight; Lipopolysaccharide (LPS); Plant immunity; Structural characterization; Xanthomonas genus