title: Different Lipopolysaccharide Branched-Chain Amino Acids Modulate Porcine Intestinal Endogenous β-Defensin Expression through the Sirt1/ERK/90RSK Pathway.
abstract: Nutritional induction of endogenous antimicrobial peptide expression is considered a promising approach to inhibit the outgrowth and infection of pathogenic microbes in mammals. The present study investigated possible regulation of porcine epithelial β-defensins in response to branched-chain amino acids (BCAA) in vivo and in vitro. BCAA treatment increased relative mRNA expression of jejunal and ileal β-defensins in weaned piglets. In IPEC-J2 cells, isoleucine, leucine, and valine could stimulate β-defensin expression, possibly associated with stimulation of ERK1/2 phosphorylation. Inhibition of Sirt1 and ERK completely blocked the activation of ERK and 90RSK protein by isoleucine, simultaneously decreasing defensin expression. BCAA stimulate expression of porcine intestinal epithelial β-defensins with isoleucine the most, potent possibly through activation of the Sirt1/ERK/90RSK signaling pathway. The β-defensins regulation of lipopolysaccharide was related with an ERK-independent pathway. BCAA modulation of endogenous defensin might be a promising approach to enhance disease resistance and intestinal health in young animals and children.
authors.affiliation: State Key Laboratory of Animal Nutrition, China Agricultural University , No. 2 Yuanmingyuan West Road, Beijing 100193, China.|College of Animal Science, Anhui Science & Technology University , No. 9 Donghua Road, Fengyang, Anhui 233100, China.
authors.forename: Man
authors.initials: M
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Animal Nutrition, China Agricultural University , No. 2 Yuanmingyuan West Road, Beijing 100193, China.
authors.forename: Shihai
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Animal Nutrition, China Agricultural University , No. 2 Yuanmingyuan West Road, Beijing 100193, China.
authors.forename: Xutong
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Animal Science, Anhui Science & Technology University , No. 9 Donghua Road, Fengyang, Anhui 233100, China.
authors.forename: Shenghe
authors.initials: S
authors.lastname: Li
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University , No. 46 Xinkang Road, Yucheng, Ya'an 625014, China.
authors.forename: Xiangbing
authors.initials: X
authors.lastname: Mao
authors.affiliation: State Key Laboratory of Animal Nutrition, China Agricultural University , No. 2 Yuanmingyuan West Road, Beijing 100193, China.
authors.forename: Xiangfang
authors.initials: X
authors.lastname: Zeng
authors.affiliation: State Key Laboratory of Animal Nutrition, China Agricultural University , No. 2 Yuanmingyuan West Road, Beijing 100193, China.
authors.forename: Shiyan
authors.initials: S
authors.lastname: Qiao
mesh_terms: D000597:Amino Acids, Branched-Chain / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000737:chemistry*; D020935:MAP Kinase Signaling System; D008297:Male; D056564:Sirtuin 1 / Q000378:metabolism*; D013552:Swine; D023083:beta-Defensins / Q000378:metabolism*
chemical_list: D000597:Amino Acids, Branched-Chain; D008070:Lipopolysaccharides; D023083:beta-Defensins; D056564:Sirtuin 1
keywords: 90RSK; ERK1/2; Sirt1; defensin; intestine; porcine