title: Phoenix dactylifera L. sap enhances wound healing in Wistar rats: Phytochemical and histological assessment.
abstract: The sap of the date palm "Lagmi" is a clear liquid, rich in sugars and minerals, with a pleasant flavour. Folk remedies based on the use of "Lagmi" for wound healing are still practiced. However, no studies investigated the relevance of "Lagmi" for wound healing. Therefore, the aim of this study was to identify the in vivo healing properties of "lagmi" on mechanically wounded wistar rats. Injured rats were divided into three groups: a first group treated by "lagmi", a second reference group processed by CICAFLORA(®) and a third untreated control group. On the 12th day of the experiment, total healing in the first group was reached, while healing was incomplete in the other groups. The sap seems to accelerate cell proliferation and contribute to faster healing with a gain of more than 30% as compared to CICAFLORA(®). Chemical Analysis of "Lagmi" showed important radical scavenging activity and high total antioxidant capacity. Features reported to help healing process and/or provides a favourable environment for tissue healing in wound sites. Extensive characterization of "Lagmi" phenolic and flavonoid compounds by High Resolution LC-MS (LC-HRESIMS) analysis indicates "Lagmi" is an important source of known anti-inflammatory compounds as well as promising wound healing candidates.
authors.affiliation: Laboratory of Plant Biotechnology, Faculty of Science, B.P. 1171, 3000, University of Sfax, 3029 Sfax, Tunisia.
authors.forename: Raed
authors.initials: R
authors.lastname: Abdennabi
authors.affiliation: Laboratory of Pharmacology, Faculty of Medicine of Sfax, University of Sfax, Tunisia.
authors.forename: Sana
authors.initials: S
authors.lastname: Bardaa
authors.affiliation: Laboratory of Cytogenetic and Reproductive Biology, Maternity and Neonatology Center of Monastir, Tunisia.
authors.forename: Meriem
authors.initials: M
authors.lastname: Mehdi
authors.affiliation: University of West of Scotland, School of Science & Sport, Paisley PA1 2BE, UK.
authors.forename: Mostafa E
authors.initials: ME
authors.lastname: Rateb
authors.affiliation: University of Aberdeen, Department of Chemistry, Meston Building, Meston Walk, Aberdeen AB24 3UE, Scotland, UK.
authors.forename: Andrea
authors.initials: A
authors.lastname: Raab
authors.affiliation: NextBiotech, 98 Rue Ali Belhouane, 3030 Agareb, Tunisia.
authors.forename: Faizah N
authors.initials: FN
authors.lastname: Alenezi
authors.affiliation: Laboratory of Pharmacology, Faculty of Medicine of Sfax, University of Sfax, Tunisia.
authors.forename: Zouheir
authors.initials: Z
authors.lastname: Sahnoun
authors.affiliation: Laboratory of Plant Biotechnology, Faculty of Science, B.P. 1171, 3000, University of Sfax, 3029 Sfax, Tunisia.
authors.forename: Neji
authors.initials: N
authors.lastname: Gharsallah
authors.affiliation: NextBiotech, 98 Rue Ali Belhouane, 3030 Agareb, Tunisia; Laboratory of Soil Biology, University of Neuchatel, 11 Rue Emile Argand, CH-2000 Neuchatel, Switzerland. Electronic address: lassaad.belbahri@unine.ch.
authors.forename: Lassaad
authors.initials: L
authors.lastname: Belbahri
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects*; D004195:Disease Models, Animal; D005419:Flavonoids / Q000302:isolation & purification; D006801:Humans; D008519:Medicine, Traditional; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification; D065749:Phoeniceae / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry*; D051381:Rats; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts
keywords: Antioxydant; Bioactive phenolic compounds; Date palm sap; LC–MS analysis; Minerals; Wound healing