title: Plasma variation and reproducibility of oxidized LDL-cholesterol and low-grade inflammation biomarkers among participants of the Malmö Diet and Cancer cohort.
abstract: BACKGROUND
  Large epidemiological studies often collect non-fasting samples, although the reliability of biomarkers may be uncertain.
  
  
  OBJECTIVE
  To explore the reliability and reproducibility of a single measurement of selected biomarkers in a sub-sample of the Malmö Diet and Cancer cohort.
  
  
  METHODS
  We estimated single- and average-measures intraclass correlation coefficients (ICC) for oxidized (ox)-LDL, interleukin (IL)-1β, IL-6, IL-8 and TNF-α.
  
  
  RESULTS
  Single-measures ICC in non-fasting samples of ox-LDL, IL-1β, IL-6, IL-8 and TNF-α were the following: 0.85, 0.71, 0.61, 0.78 and 0.66 for men, and 0.67, 0.81, 0.87, 0.69 and 0.81 for women. Biomarkers at non-fasting and fasting samples were highly correlated (all r > 0.80).
  
  
  CONCLUSIONS
  The observed ICC suggest that most of the examined biomarkers (non-fasting blood) would allow meaningful analysis in epidemiological studies.
authors.affiliation: a Department of Clinical Sciences in Malmö , Clinical Research Centre (CRC), Lund University , Lund , Sweden.
authors.forename: Joana
authors.initials: J
authors.lastname: Alves Dias
authors.affiliation: a Department of Clinical Sciences in Malmö , Clinical Research Centre (CRC), Lund University , Lund , Sweden.
authors.forename: Sophie
authors.initials: S
authors.lastname: Hellstrand
authors.affiliation: a Department of Clinical Sciences in Malmö , Clinical Research Centre (CRC), Lund University , Lund , Sweden.
authors.forename: Ulrika
authors.initials: U
authors.lastname: Ericson
authors.affiliation: a Department of Clinical Sciences in Malmö , Clinical Research Centre (CRC), Lund University , Lund , Sweden.
authors.forename: Bo
authors.initials: B
authors.lastname: Gullberg
authors.affiliation: a Department of Clinical Sciences in Malmö , Clinical Research Centre (CRC), Lund University , Lund , Sweden.
authors.forename: Jan
authors.initials: J
authors.lastname: Nilsson
authors.affiliation: a Department of Clinical Sciences in Malmö , Clinical Research Centre (CRC), Lund University , Lund , Sweden.
authors.forename: Ragnar
authors.initials: R
authors.lastname: Alm
authors.affiliation: a Department of Clinical Sciences in Malmö , Clinical Research Centre (CRC), Lund University , Lund , Sweden.
authors.forename: Margaretha
authors.initials: M
authors.lastname: Persson
authors.affiliation: a Department of Clinical Sciences in Malmö , Clinical Research Centre (CRC), Lund University , Lund , Sweden.
authors.forename: Gunnar
authors.initials: G
authors.lastname: Engström
authors.affiliation: a Department of Clinical Sciences in Malmö , Clinical Research Centre (CRC), Lund University , Lund , Sweden.
authors.forename: Gunilla
authors.initials: G
authors.lastname: Nordin Fredrikson
authors.affiliation: a Department of Clinical Sciences in Malmö , Clinical Research Centre (CRC), Lund University , Lund , Sweden.
authors.forename: Bo
authors.initials: B
authors.lastname: Hedblad
authors.affiliation: a Department of Clinical Sciences in Malmö , Clinical Research Centre (CRC), Lund University , Lund , Sweden.
authors.forename: Elisabet
authors.initials: E
authors.lastname: Wirfält
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D015331:Cohort Studies; D004032:Diet; D006801:Humans; D007249:Inflammation / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000097:blood* / Q000276:immunology; D015203:Reproducibility of Results; D013548:Sweden
chemical_list: D014408:Biomarkers, Tumor; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein
keywords: Biomarkers of inflammation; ICC; cytokines; epidemiology; variation