title: Establishment and characterization of HROC69 - a Crohn´s related colonic carcinoma cell line and its matched patient-derived xenograft.
abstract: Colitis-associated colorectal cancer (CAC) seems to be a rather unique entity and differs in its genetic alterations, tumour formation capacities, and clinical features from sporadic colorectal carcinoma. Most descriptions about tumour biology of CAC refer to ulcerative colitis; data about Crohn´s colitis related carcinomas are scarce. The majority of patients with Crohn´s disease are under immunosuppression which generates a different environment for tumour growth. We first describe the clinical case of a fast growing CAC in a long-term immunosuppressed patient with Crohn´s disease and successful establishment and characterization of carcinoma cell lines along with their corresponding patient-derived xenograft. Subsequently, these tumor models were molecularly and functionally analysed. Beside numerous chromosomal alterations, mutations in TP53, APC, PTEN and SMAD3 were identified. The cell lines express numerous cancer testis antigens, surface molecules involved in immune evasion but low levels of HLA class I molecules. They show strong invasive but in comparison weak migratory activity. The present work is the first description of patient-derived in vitro and in vivo models for CAC from a Crohn´s disease patient. They might be valuable tools for analysis of genetic and epigenetic alterations, biomarker identification, functional testing, including response prediction, and the development of specific therapeutical strategies.
authors.affiliation: University Medicine Rostock, Department of General-, Thoracic-, Vascular- and Transplantation Surgery, Rostock, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Kuehn
authors.affiliation: University Medicine Rostock, Department of General Surgery, Molecular Oncology and Immunotherapy, Rostock, Germany.
authors.forename: Christina S
authors.initials: CS
authors.lastname: Mullins
authors.affiliation: University Medicine Rostock, Department of General Surgery, Molecular Oncology and Immunotherapy, Rostock, Germany.
authors.forename: Mathias
authors.initials: M
authors.lastname: Krohn
authors.affiliation: University Medicine Rostock, Department of General-, Thoracic-, Vascular- and Transplantation Surgery, Rostock, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Harnack
authors.affiliation: University Medicine Rostock, Institute of Toxicology and Pharmacology, Rostock, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Ramer
authors.affiliation: University Medical Center Mainz, Department of Toxicology, Mainz, Germany.
authors.forename: Oliver H
authors.initials: OH
authors.lastname: Krämer
authors.affiliation: University Medicine Rostock, Department of General-, Thoracic-, Vascular- and Transplantation Surgery, Rostock, Germany.
authors.forename: Ernst
authors.initials: E
authors.lastname: Klar
authors.affiliation: University Medicine Rostock, Institute of Pathology, Rostock, Germany.
authors.forename: Maja
authors.initials: M
authors.lastname: Huehns
authors.affiliation: University Medicine Rostock, Department of General Surgery, Molecular Oncology and Immunotherapy, Rostock, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Linnebacher
mesh_terms: D025601:Adenomatous Polyposis Coli Protein / Q000235:genetics / Q000378:metabolism; D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D002277:Carcinoma / Q000209:etiology / Q000235:genetics / Q000473:pathology*; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D003110:Colonic Neoplasms / Q000209:etiology / Q000235:genetics / Q000473:pathology*; D003424:Crohn Disease / Q000150:complications / Q000473:pathology*; D005805:Genes, MHC Class I; D045325:HCT116 Cells; D064593:Heterografts / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism; D051900:Smad3 Protein / Q000235:genetics / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: C555337:APC protein, human; D025601:Adenomatous Polyposis Coli Protein; D014408:Biomarkers, Tumor; C497790:SMAD3 protein, human; D051900:Smad3 Protein; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D051059:PTEN Phosphohydrolase