title: Glycopolymeric gel stabilized N-succinyl chitosan beads for controlled doxorubicin delivery.
abstract: Here we report the synthesis and study of N-succinyl chitosan based hydrogel beads, stabilized with glycopolymeric network (NSC/Glc-gel) for application in anticancer drug delivery of doxorubicin (DOX). The bio-recognition of lectins by NSC/Glc-gel bead was also studied by UV-vis spectrophotometry. The beads were characterized using FT-IR, SEM and Thermogravimetric analysis. The extent of DOX loading was proportional to the degree of succinylation and the swelling kinetics of the beads showed pH dependency. The beads exhibited sustained release of DOX over a period of more than 15 days in an acidic pH, mimicking the microenvironment of tumor cells, and even lesser release at physiological pH. Release exponent 'n' derived from Korsmeyer-Peppas model implied that NSC88/Glc-gel (88% succinylation of chitosan) beads followed fickian diffusion controlled release mechanism whereas NSC75/Glc-gel (75% succinylation of chitosan) beads follow zero order release profile. The synthesized beads also displayed specificity to lectin Concanavalin A.
authors.affiliation: Radiation and Photochemistry Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India; Homi Bhabha National Institute, Trombay, Mumbai 400085, India.
authors.forename: Juby K
authors.initials: JK
authors.lastname: Ajish
authors.affiliation: Bio-Organic Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India.
authors.forename: K S
authors.initials: KS
authors.lastname: Ajish Kumar
authors.affiliation: Bio-Organic Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India. Electronic address: schatt@barc.gov.in.
authors.forename: S
authors.initials: S
authors.lastname: Chattopadhyay
authors.affiliation: Radiation and Photochemistry Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India. Electronic address: manmoku@barc.gov.in.
authors.forename: Manmohan
authors.initials: M
authors.lastname: Kumar
mesh_terms: D000178:Acrylamides / Q000138:chemical synthesis / Q000737:chemistry*; D000818:Animals; D010367:Arachis; D002417:Cattle; D048271:Chitosan / Q000138:chemical synthesis / Q000737:chemistry*; D003208:Concanavalin A / Q000737:chemistry; D004317:Doxorubicin / Q000008:administration & dosage / Q000737:chemistry*; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry*; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D019887:Peanut Agglutinin / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D000178:Acrylamides; D004337:Drug Carriers; D020100:Hydrogels; C118639:N-succinyl-chitosan; D019887:Peanut Agglutinin; D003208:Concanavalin A; D012710:Serum Albumin, Bovine; D004317:Doxorubicin; D048271:Chitosan
keywords: Beads; Doxorubicin; Glycopolymer; Hydrogel; N-Succinyl chitosan