title: Prenylation differentially inhibits insulin-dependent immediate early gene mRNA expression.
abstract: Increased activity of prenyl transferases is observed in pathological states of insulin resistance, diabetes, and obesity. Thus, functional inhibitors of farnesyl transferase (FTase) and geranylgeranyl transferase (GGTase) may be promising therapeutic treatments. We previously identified insulin responsive genes from a rat H4IIE hepatoma cell cDNA library, including β-actin, EGR1, Pip92, c-fos, and Hsp60. In the present study, we investigated whether acute treatment with FTase and GGTase inhibitors would alter insulin responsive gene initiation and/or elongation rates. We observed differential regulation of insulin responsive gene expression, suggesting a differential sensitivity of these genes to one or both of the specific protein prenylation inhibitors.
authors.affiliation: University of Alabama at Birmingham, Department of Pathology, Division of Molecular and Cellular Pathology, Birmingham, AL 35294, USA.
authors.forename: J Lee
authors.initials: JL
authors.lastname: Franklin
authors.affiliation: University of Alabama at Birmingham, Department of Biology, Birmingham, AL 35294, USA.
authors.forename: Maggie O
authors.initials: MO
authors.lastname: Amsler
authors.affiliation: University of Alabama at Birmingham, Department of Pathology, Division of Molecular and Cellular Pathology, Birmingham, AL 35294, USA; Veterans Administration Medical Center, Birmingham, AL 35294, USA. Electronic address: messinaj@uab.edu.
authors.forename: Joseph L
authors.initials: JL
authors.lastname: Messina
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D018834:Chaperonin 60 / Q000378:metabolism; D051766:Early Growth Response Protein 1 / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology*; D024101:Mitochondrial Proteins / Q000378:metabolism; D054876:Prenylation / Q000187:drug effects / Q000502:physiology*; D011506:Proteins / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects / Q000502:physiology*
chemical_list: D000199:Actins; D018834:Chaperonin 60; D051766:Early Growth Response Protein 1; C486110:Egr1 protein, rat; C557067:Hspd1 protein, rat; D007328:Insulin; D024101:Mitochondrial Proteins; D011506:Proteins; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; C484022:pip92 protein, rat
keywords: Gene expression; Immediate early genes; Insulin; Prenylation; Transcription