title: Extracellular superoxide dismutase ameliorates house dust mite-induced atopic dermatitis-like skin inflammation and inhibits mast cell activation in mice.
abstract: Extracellular superoxide dismutase (EC-SOD) is an enzyme that catalyses the dismutation of superoxide anions. It has multiple functions, such as reactive oxygen species scavenging, anti-angiogenic, anti-inflammatory, antichemotatic and antitumor activities. Recently, we demonstrated that EC-SOD inhibits ovalbumin-induced allergic airway inflammation in mice. However, the anti-allergic effect of EC-SOD on skin tissue and the role of EC-SOD in mast cells, which are important for allergic responses, have not been well studied. In this study, we investigated whether EC-SOD can alleviate atopic dermatitis in mice and inhibit mast cell activation. Treatment with human recombinant EC-SOD ameliorated house dust mite-induced atopic dermatitis in mice. Furthermore, the levels of pro-allergic cytokine gene expression and histamine release increased in EC-SOD KO mast cells and decreased in EC-SOD overexpressing mast cells, suggesting that EC-SOD inhibits mast cell activation. Consistently, a passive cutaneous anaphylaxis experiment showed more blood leakage from EC-SOD KO mouse ear skin, implying that the lack of EC-SOD increases allergic responses. These results suggest that EC-SOD inhibits mast cell activation and atopic dermatitis and that the loss of EC-SOD causes more severe allergic responses, implying that EC-SOD might be a good drug candidate for treatment of allergic disorders, such as atopic dermatitis.
authors.affiliation: Department of Dermatology, College of Medicine, The Catholic University of Korea, Seoul, South Korea.|Department of Otolaryngology, School of Medicine, Ajou University, Suwon, Korea.
authors.forename: Yun Sang
authors.initials: YS
authors.lastname: Lee
authors.affiliation: Department of Dermatology, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
authors.forename: Jung-Hye
authors.initials: JH
authors.lastname: Choi
authors.affiliation: Department of Dermatology, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul, South Korea.
authors.forename: Han-Woong
authors.initials: HW
authors.lastname: Lee
authors.affiliation: Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul, South Korea.
authors.forename: Weontae
authors.initials: W
authors.lastname: Lee
authors.affiliation: Department of Systems Biology, College of life Science and Biotechnology, Yonsei University, Seoul, South Korea.
authors.forename: Woo Taek
authors.initials: WT
authors.lastname: Kim
authors.affiliation: Department of Dermatology, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
authors.forename: Tae-Yoon
authors.initials: TY
authors.lastname: Kim
mesh_terms: D000818:Animals; D003876:Dermatitis, Atopic / Q000188:drug therapy* / Q000276:immunology; D004353:Drug Evaluation, Preclinical; D008407:Mast Cells / Q000187:drug effects*; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D039981:Pyroglyphidae / Q000276:immunology*; D013482:Superoxide Dismutase / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D013482:Superoxide Dismutase
keywords: NC/Nga mouse; atopic dermatitis; extracellular superoxide dismutase; mast cells