title: Comparison of protein- and polysaccharide-based nanoparticles for cancer therapy: synthesis, characterization, drug release, and interaction with a breast cancer cell line.
abstract: In this study, human serum albumin (HSA) was used as a protein-based material and poly (3-hydroxybutyrate) (PHB)-carboxymethyl chitosan (CMCh) as a polysaccharide-based material for the production of nanoparticles to be used as nanocarriers in cancer therapy. HSA and PHB-CMCh nanoparticles were prepared and characterized with a Zeta Sizer, Fourier transform infrared spectroscopy, scanning electron microscopy, and atomic force microscope. The effects of the pH value of the suspending medium and the amounts of crosslinker and polymer concentration on nanoparticle size and size distribution were investigated. The anticancer-agent etoposide was used as a model drug and encapsulated in nanoparticles to obtain drug release profiles. The entrapment efficiency of HSA nanoparticles was found to be greater than that of PHB-CMCh nanoparticles. To achieve "active" targeting of cancer cells, the nanoparticles were modified with concanavalin A. In the final step of the study, the interaction of nanoparticles with cancer cells was investigated in cytotoxicity and cellular uptake studies.
authors.affiliation: a Biology Department, Polatlı Art and Science Faculty , Gazi University , Ankara , Turkey.|b Nanotechnology & Nanomedicine Division Hacettepe University , Ankara , Turkey.
authors.forename: Öznur
authors.initials: Ö
authors.lastname: Akbal
authors.affiliation: b Nanotechnology & Nanomedicine Division Hacettepe University , Ankara , Turkey.|c Biology Department , Aksaray University , Aksaray , Turkey.
authors.forename: Ebru
authors.initials: E
authors.lastname: Erdal
authors.affiliation: d Chemistry Department, Biochemistry Division , Hacettepe University , Ankara , Turkey.
authors.forename: Tayfun
authors.initials: T
authors.lastname: Vural
authors.affiliation: e Bioengineering Department , Cyprus International University , Haspolat, Nicosia, Northern Cyprus , Turkey.
authors.forename: Doğa
authors.initials: D
authors.lastname: Kavaz
authors.affiliation: d Chemistry Department, Biochemistry Division , Hacettepe University , Ankara , Turkey.|f Hacettepe University Advanced Technologies Application and Research Center, HUNITEK , Ankara , Turkey.
authors.forename: Emir Baki
authors.initials: EB
authors.lastname: Denkbaş
mesh_terms: D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D006801:Humans; D006885:Hydroxybutyrates / Q000737:chemistry / Q000494:pharmacology; D061986:MCF-7 Cells; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D000091402:Prohibitins; D012709:Serum Albumin / Q000737:chemistry / Q000494:pharmacology; D000094122:Polyhydroxybutyrates
chemical_list: D048271:Chitosan; D006885:Hydroxybutyrates; D011091:Polyesters; D000091402:Prohibitins; D012709:Serum Albumin; C000717328:PHB protein, human; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
keywords: Concanavalin A; HSA nanoparticle; PHB–CMCh nanoparticle; etoposide