title: Isolation and functional characterization of a cold responsive phosphatidylinositol transfer-associated protein, ZmSEC14p, from maize (Zea may L.).
abstract: A Sec14-like protein, ZmSEC14p , from maize was structurally analyzed and functionally tested. Overexpression of ZmSEC14p in transgenic Arabidopsis conferred tolerance to cold stress. Sec14-like proteins are involved in essential biological processes, such as phospholipid metabolism, signal transduction, membrane trafficking, and stress response. Here, we reported a phosphatidylinositol transfer-associated protein, ZmSEC14p (accession no. KT932998), isolated from a cold-tolerant maize inbred line using the cDNA-AFLP approach and RACE-PCR method. Full-length cDNA that consisted of a single open reading frame (ORF) encoded a putative polypeptide of 295 amino acids. The ZmSEC14p protein was mainly localized in the nucleus, and its transcript was induced by cold, salt stresses, and abscisic acid (ABA) treatment in maize leaves and roots. Overexpression of ZmSEC14p in transgenic Arabidopsis conferred tolerance to cold stress. This tolerance was primarily displayed by the increased germination rate, root length, plant survival rate, accumulation of proline, activities of antioxidant enzymes, and the reduction of oxidative damage by reactive oxygen species (ROS). ZmSEC14p overexpression regulated the expression of phosphoinositide-specific phospholipase C, which cleaves phosphatidylinositol 4,5-bisphosphate (PIP2) and generates second messengers (inositol 1,4,5-trisphosphate and 1,2-diacylglycerol) in the phosphoinositide signal transduction pathways. Moreover, up-regulation of some stress-responsive genes such as CBF3, COR6.6, and RD29B in transgenic plants under cold stress could be a possible mechanism for enhancing cold tolerance. Taken together, this study strongly suggests that ZmSEC14p plays an important role in plant tolerance to cold stress.
authors.affiliation: College of Plant Science, Jilin University, Changchun, 130062, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Plant Science, Jilin University, Changchun, 130062, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Shan
authors.affiliation: College of Plant Science, Jilin University, Changchun, 130062, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Xue
authors.affiliation: College of Plant Science, Jilin University, Changchun, 130062, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wu
authors.affiliation: College of Plant Science, Jilin University, Changchun, 130062, China.
authors.forename: Shengzhong
authors.initials: S
authors.lastname: Su
authors.affiliation: College of Plant Science, Jilin University, Changchun, 130062, China.
authors.forename: Shipeng
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Plant Science, Jilin University, Changchun, 130062, China.
authors.forename: Hongkui
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Plant Science, Jilin University, Changchun, 130062, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: College of Plant Science, Jilin University, Changchun, 130062, China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Plant Science, Jilin University, Changchun, 130062, China. yuanyp@jlu.edu.cn.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Yuan
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics; D000595:Amino Acid Sequence; D000975:Antioxidants / Q000378:metabolism; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D003080:Cold Temperature; D005615:Freezing; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D018525:Germination / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000378:metabolism; D019697:Onions / Q000166:cytology; D054801:Phosphoinositide Phospholipase C / Q000378:metabolism; D049294:Phospholipid Transfer Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D010802:Phylogeny; D019441:Plant Epidermis / Q000166:cytology / Q000187:drug effects; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011392:Proline / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012639:Seeds / Q000187:drug effects / Q000254:growth & development; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D013347:Subcellular Fractions / Q000378:metabolism; D003313:Zea mays / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000502:physiology
chemical_list: D000975:Antioxidants; D049294:Phospholipid Transfer Proteins; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D049452:Green Fluorescent Proteins; D012965:Sodium Chloride; D000040:Abscisic Acid; D011392:Proline; D054801:Phosphoinositide Phospholipase C
keywords: Cold stress; Functional analysis; Gene expression; Zea mays; ZmSEC14p