title: Cucumarioside A2-2 causes changes in the morphology and proliferative activity in mouse spleen.
abstract: The immunomodulatory effect of triterpene glycoside cucumarioside A2-2 (CA2-2), isolated from the Far Eastern sea cucumber Cucumaria japonica, on the mouse spleen was investigated in comparison with lipopolysaccharide (LPS). It has been shown that the intraperitoneal (i.p.) glycoside administration did not influence on splenic weights, while the statistically significant increase in splenic weight was observed after LPS administration. Changes in the ratio of red to white pulp after CA2-2 or LPS administration were observed. The proportion of splenic white pulp after glycoside or LPS administration increased by up to 34% and 36%, respectively. A detailed study of the distribution of the РСNA (Proliferating Cell Nuclear Antigen) marker showed that the proliferative activity in the white pulp under CA2-2 and LPS influence increased 2.07 and 2.24 times, respectively. The localization of PCNA-positive nuclei in the white pulp region, as well as their dimensional characteristics, suggests that a large proportion of the proliferating cell population consisted of B cells. The mass spectrometry profiles of spleen peptide/protein homogenate were obtained using the MALDI-TOF-MS (Matrix -Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry) approach. It was found that i.p. stimulation of animals with CA2-2 or LPS leads to marked changes in the intensity of revealed characteristic peaks of peptides/proteins after exposure to immunostimulants.
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia. Electronic address: pislyagin@hotmail.com.
authors.forename: E A
authors.initials: EA
authors.lastname: Pislyagin
authors.affiliation: A.V.Zhirmunsky Institute of Marine Biology, Far Eastern Branch of Russian Academy of Science, Vladivostok, Russia; School of Biomedicine, Far Eastern Federal University, Vladivostok, Russia.
authors.forename: I V
authors.initials: IV
authors.lastname: Manzhulo
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia.
authors.forename: P S
authors.initials: PS
authors.lastname: Dmitrenok
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia.
authors.forename: D L
authors.initials: DL
authors.lastname: Aminin
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D005260:Female; D007155:Immunologic Factors / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008807:Mice, Inbred BALB C; D012503:Saponins / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000276:immunology
chemical_list: D007155:Immunologic Factors; D008070:Lipopolysaccharides; D012503:Saponins; C539161:cucumarioside A2-2
keywords: Immunomodulation; LPS; PCNA; Sea cucumbers; Spleen; Triterpene glycosides