title: The combination of three molecular markers can be a valuable predictive tool for the prognosis of hepatocellular carcinoma patients.
abstract: Based on molecular profiling, several prognostic markers for HCC are also used in clinic, but only a few genes have been identified as useful. We collected 72 post-operative liver cancer tissue samples. Genes expression were tested by RT-PCR. Multilayer perceptron and discriminant analysis were built, and their ability to predict the prognosis of HCC patients were tested. Receiver operating characteristic (ROC) analysis was performed and multivariate analysis with Cox's Proportional Hazard Model was used for confirming the markers'predictive efficiency for HCC patients'survival. A simple risk scoring system devised for further predicting the prognosis of liver tumor patients. Multilayer perceptron and discriminant analysis showed a very strong predictive value in evaluating liver cancer patients'prognosis. Cox multivariate regression analysis demonstrated that DUOX1, GLS2, FBP1 and age were independent risk factors for the prognosis of HCC patients after surgery. Finally, the risk scoring system revealed that patients whose total score >1 and >3 are more likely to relapse and die than patients whose total score ≤1 and ≤3. The three genes model proposed proved to be highly predictive of the HCC patients' prognosis. Implementation of risk scoring system in clinical practice can help in evaluating survival of HCC patients after operation.
authors.affiliation: Department of Infectious Diseases and Hepatology, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Sheng-Sen
authors.initials: SS
authors.lastname: Chen
authors.affiliation: Department of Infectious Diseases and Hepatology, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Kang-Kang
authors.initials: KK
authors.lastname: Yu
authors.affiliation: Department of Infectious Diseases and Hepatology, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Qing-Xia
authors.initials: QX
authors.lastname: Ling
authors.affiliation: Department of Infectious Diseases and Hepatology, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of Infectious Diseases and Hepatology, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Infectious Diseases and Hepatology, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Zheng
authors.affiliation: Department of Infectious Diseases and Hepatology, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Su-Xia
authors.initials: SX
authors.lastname: Bao
authors.affiliation: Department of Infectious Diseases and Hepatology, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Cheng
authors.affiliation: Department of Infectious Diseases and Hepatology, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Meng-Qi
authors.initials: MQ
authors.lastname: Zhu
authors.affiliation: Department of Infectious Diseases and Hepatology, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Ming-Quan
authors.initials: MQ
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D000074623:Dual Oxidases; D005260:Female; D006597:Fructose-Bisphosphatase / Q000235:genetics; D005972:Glutaminase / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D008962:Models, Theoretical; D019255:NADPH Oxidases / Q000235:genetics; D011379:Prognosis; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D000074623:Dual Oxidases; D019255:NADPH Oxidases; C431513:DUOX1 protein, human; D006597:Fructose-Bisphosphatase; C586504:GLS2 protein, human; D005972:Glutaminase