title: Molar concentration from sequential 2-D water-window X-ray ptychography and X-ray fluorescence in hydrated cells.
abstract: Recent developments in biological X-ray microscopy have allowed structural information and elemental distribution to be simultaneously obtained by combining X-ray ptychography and X-ray fluorescence microscopy. Experimentally, these methods can be performed simultaneously; however, the optimal conditions for each measurement may not be compatible. Here, we combine two distinct measurements of ultrastructure and elemental distribution, with each measurement performed under optimised conditions. By combining optimised ptychography and fluorescence information we are able to determine molar concentrations from two-dimensional images, allowing an investigation into the interactions between the environment sensing filopodia in fibroblasts and extracellular calcium. Furthermore, the biological ptychography results we present illustrate a point of maturity where the technique can be applied to solve significant problems in structural biology.
authors.affiliation: Australian Synchrotron, 800 Blackburn Rd, Clayton, 3168, Australia.|ARC Centre of Excellence for Advanced Molecular Imaging, La Trobe Institute for Molecular Sciences, La Trobe University, Bundoora, 3086, Australia.
authors.forename: M W M
authors.initials: MW
authors.lastname: Jones
authors.affiliation: Monash Micro Imaging, Hudson Institute of Medical Research, 27-31 Wright Street, Clayton, 3168, Australia.
authors.forename: K D
authors.initials: KD
authors.lastname: Elgass
authors.affiliation: Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Victoria 3086, Australia.
authors.forename: M D
authors.initials: MD
authors.lastname: Junker
authors.affiliation: Australian Synchrotron, 800 Blackburn Rd, Clayton, 3168, Australia.
authors.forename: M D
authors.initials: MD
authors.lastname: de Jonge
authors.affiliation: Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Victoria 3086, Australia.
authors.forename: G A
authors.initials: GA
authors.lastname: van Riessen
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004602:Elements; D005109:Extracellular Matrix / Q000737:chemistry*; D005347:Fibroblasts / Q000648:ultrastructure*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D051379:Mice; D008853:Microscopy / Q000379:methods*; D013052:Spectrometry, X-Ray Emission / Q000379:methods*; D014961:X-Ray Diffraction / Q000379:methods*
chemical_list: D004602:Elements