title: Dynamic and quantitative assessment of blood coagulation using optical coherence elastography.
abstract: Reliable clot diagnostic systems are needed for directing treatment in a broad spectrum of cardiovascular diseases and coagulopathy. Here, we report on non-contact measurement of elastic modulus for dynamic and quantitative assessment of whole blood coagulation using acoustic radiation force orthogonal excitation optical coherence elastography (ARFOE-OCE). In this system, acoustic radiation force (ARF) is produced by a remote ultrasonic transducer, and a shear wave induced by ARF excitation is detected by the optical coherence tomography (OCT) system. During porcine whole blood coagulation, changes in the elastic property of the clots increase the shear modulus of the sample, altering the propagating velocity of the shear wave. Consequently, dynamic blood coagulation status can be measured quantitatively by relating the velocity of the shear wave with clinically relevant coagulation metrics, including reaction time, clot formation kinetics and maximum shear modulus. The results show that the ARFOE-OCE is sensitive to the clot formation kinetics and can differentiate the elastic properties of the recalcified porcine whole blood, blood added with kaolin as an activator, and blood spiked with fibrinogen.
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, China.|Beckman Laser Institute, University of California, Irvine, Irvine, California 92612, USA.
authors.forename: Xiangqun
authors.initials: X
authors.lastname: Xu
authors.affiliation: Beckman Laser Institute, University of California, Irvine, Irvine, California 92612, USA.
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Beckman Laser Institute, University of California, Irvine, Irvine, California 92612, USA.|Department of Biomedical Engineering, University of California, Irvine, Irvine, California 92697, USA.
authors.forename: Zhongping
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000502:physiology*; D054459:Elasticity Imaging Techniques; D005340:Fibrinogen / Q000378:metabolism; D006801:Humans; D019047:Phantoms, Imaging; D013552:Swine; D013927:Thrombosis / Q000000981:diagnostic imaging* / Q000473:pathology; D041623:Tomography, Optical Coherence
chemical_list: D005340:Fibrinogen