title: Antiallergic Activity of Ethanol Extracts of Arctium lappa L. Undried Roots and Its Active Compound, Oleamide, in Regulating FcεRI-Mediated and MAPK Signaling in RBL-2H3 Cells.
abstract: The antiallergic potential of Arctium lappa L. was investigated in Sprague-Dawley rats, ICR mice, and RBL-2H3 cells. Ethanol extract (90%) of A. lappa (ALE, 100 μg/mL) inhibited the degranulation rate by 52.9%, determined by the level of β-hexosaminidase. ALE suppressed passive cutaneous anaphylaxis (PCA) in rats and attenuated anaphylaxis and histamine release in mice. To identify the active compound of ALE, we subsequently fractionated and determined the level of β-hexosaminidase in all subfractions. Oleamide was identified as an active compound of ALE, which attenuated the secretion of histamine and the production of tumor necrosis factor (TNF)-α and interleukin-4 (IL-4) in cells treated with compound 48/80 or A23187/phorbol myristate acetate (PMA). Oleamide suppressed FcεRI-tyrosine kinase Lyn-mediated pathway, c-Jun N-terminal kinases (JNK/SAPK), and p38 mitogen-activated protein kinases (p38-MAPKs). These results showed that ALE and oleamide attenuated allergic reactions and should serve as a platform to search for compounds with antiallergic activity.
authors.affiliation: School of Life Sciences and Biotechnology, Kyungpook National University , Daegu 41566, Republic of Korea.
authors.forename: Woong-Suk
authors.initials: WS
authors.lastname: Yang
authors.affiliation: Cardiovascular and Metabolic Disease Center and Department of Health Sciences and Technology, Inje University , Busan 47392, Republic of Korea.
authors.forename: Sung Ryul
authors.initials: SR
authors.lastname: Lee
authors.affiliation: Department of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University , Yongin-si 17104, Republic of Korea.
authors.forename: Yong Joon
authors.initials: YJ
authors.lastname: Jeong
authors.affiliation: Department of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University , Yongin-si 17104, Republic of Korea.
authors.forename: Dae Won
authors.initials: DW
authors.lastname: Park
authors.affiliation: Department of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University , Yongin-si 17104, Republic of Korea.
authors.forename: Young Mi
authors.initials: YM
authors.lastname: Cho
authors.affiliation: Radiation Health Research Institute, Korea Hydro & Nuclear Power Co., Ltd. , Seongnam-si 13605, Republic of Korea.
authors.forename: Hae Mi
authors.initials: HM
authors.lastname: Joo
authors.affiliation: Department of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University , Yongin-si 17104, Republic of Korea.
authors.forename: Inhye
authors.initials: I
authors.lastname: Kim
authors.affiliation: School of Life Sciences and Biotechnology, Kyungpook National University , Daegu 41566, Republic of Korea.
authors.forename: Young-Bae
authors.initials: YB
authors.lastname: Seu
authors.affiliation: Department of Herbal Medicine Resource, Kangwon National University , Samcheok 25913, Republic of Korea.
authors.forename: Eun-Hwa
authors.initials: EH
authors.lastname: Sohn
authors.affiliation: Department of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University , Yongin-si 17104, Republic of Korea.
authors.forename: Se Chan
authors.initials: SC
authors.lastname: Kang
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000008:administration & dosage* / Q000302:isolation & purification; D031170:Arctium / Q000737:chemistry*; D002460:Cell Line; D006632:Histamine / Q000276:immunology; D006801:Humans; D006967:Hypersensitivity / Q000188:drug therapy* / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008813:Mice, Inbred ICR; D020929:Mitogen-Activated Protein Kinase Kinases / Q000276:immunology; D009829:Oleic Acids / Q000008:administration & dosage* / Q000302:isolation & purification; D010936:Plant Extracts / Q000008:administration & dosage* / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D017455:Receptors, IgE / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D018926:Anti-Allergic Agents; D009829:Oleic Acids; D010936:Plant Extracts; D017455:Receptors, IgE; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; C029407:oleylamide; D006632:Histamine; D020929:Mitogen-Activated Protein Kinase Kinases
keywords: Arctium lappa L; Lyn; RBL-2H3 cell; antiallergic; oleamide