title: A GC/MS method for determination of succinylacetone in Arabidopsis thaliana.
abstract: Succinylacetone was known to be a toxic metabolite of tyrosine in human and animals caused by blockage of the final step in tyrosine degradation pathway, but its existence in plant was unclear though the metabolic disturbance of tyrosine was also found in plant. A GC-MS method for determination of succinylacetone in Arabidopsis thaliana was developed for the first time. Both oximation and silylation were applied in the derivation procedure, and a low-temperature condition before completion of oximation was found to be necessary to obtain good linearity of the calibration curve due to the thermolability of succinylacetone. The specific chromatogram pattern formed by the four isomers of succinylacetone derivatives provided a helpful feature for its identification. The detection limit of the proposed method was 0.25 ppm in A. thaliana. The recoveries were between 95.4 and 109.3 % with the coefficient of variation ranging from 4.36 to 7.81 % for intra-day assays and 6.47 to 8.52 % for inter-day assays. Application to wild-type and the short-day sensitive cell death 1 mutant of A. thaliana represented an obvious correlation between the measured amount of succinylacetone and wilting symptom, suggesting the proposed method could be a powerful tool in further study on toxicology of succinylacetone and tyrosine catabolism in plants.
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan, 410083, China.
authors.forename: Lizi
authors.initials: L
authors.lastname: Zhou
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan, 410083, China.
authors.forename: Zhaoguang
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha, Hunan, 410128, China.
authors.forename: Tiantian
authors.initials: T
authors.lastname: Zhi
authors.affiliation: Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha, Hunan, 410128, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan, 410083, China.
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Wang
authors.affiliation: Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha, Hunan, 410128, China. rencm@hunau.net.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Ren
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan, 410083, China. qiubo1983@csu.edu.cn.
authors.forename: Bo
authors.initials: B
authors.lastname: Qiu
mesh_terms: D017360:Arabidopsis / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006537:Heptanoates / Q000032:analysis*; D057230:Limit of Detection; D015203:Reproducibility of Results
chemical_list: D006537:Heptanoates; C020804:succinylacetone
keywords: Arabidopsis thaliana; Gas chromatography; Mass spectrometry; Succinylacetone; Tyrosine catabolism