title: Anti-inflammatory activity of myricetin from Diospyros lotus through suppression of NF-κB and STAT1 activation and Nrf2-mediated HO-1 induction in lipopolysaccharide-stimulated RAW264.7 macrophages.
abstract: Diospyros lotus is traditionally used for the treatment of diabetes, diarrhea, tumor, and hypertension. The purpose of this study was to investigate the anti-inflammatory effect and underlying molecular mechanisms of myricetin in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Myricetin dose-dependently suppressed the production of pro-inflammatory mediators (NO, iNOS, PGE2, and COX-2) in LPS-stimulated RAW264.7 macrophages. Myricetin administration decreased the production of NO, iNOS, TNF-α, IL-6, and IL-12 in mice. Myricetin decreased NF-κB activation by suppressing the degradation of IκBα, nuclear translocation of p65 subunit of NF-κB, and NF-κB DNA binding activity in LPS-stimulated RAW264.7 macrophages. Moreover, myricetin attenuated the phosphorylation of STAT1 and the production of IFN-β in LPS-stimulated RAW264.7 macrophages. Furthermore, myricetin induced the expression of HO-1 through Nrf2 translocation. In conclusion, these results suggest that myricetin inhibits the production of pro-inflammatory mediators through the suppression of NF-κB and STAT1 activation and induction of Nrf2-mediated HO-1 expression in LPS-stimulated RAW264.7 macrophages.
authors.affiliation: a Ato Q&A Corporation , Jeonju , Republic of Korea.|b Department of Health Care & Science , Jeonju University , Jeonju , Republic of Korea.
authors.forename: Byoung Ok
authors.initials: BO
authors.lastname: Cho
authors.affiliation: a Ato Q&A Corporation , Jeonju , Republic of Korea.
authors.forename: Hong Hua
authors.initials: HH
authors.lastname: Yin
authors.affiliation: c Advanced Radiation Technology Institute , Korea Atomic Energy Research Institute , Jeongeup , Republic of Korea.
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Park
authors.affiliation: c Advanced Radiation Technology Institute , Korea Atomic Energy Research Institute , Jeongeup , Republic of Korea.
authors.forename: Eui Baek
authors.initials: EB
authors.lastname: Byun
authors.affiliation: d Department of Pharmaceutical Science & Engineering , Seowon University , Cheongju , Republic of Korea.
authors.forename: Hun Yong
authors.initials: HY
authors.lastname: Ha
authors.affiliation: a Ato Q&A Corporation , Jeonju , Republic of Korea.|b Department of Health Care & Science , Jeonju University , Jeonju , Republic of Korea.
authors.forename: Seon Il
authors.initials: SI
authors.lastname: Jang
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D029788:Diospyros / Q000737:chemistry*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D005786:Gene Expression Regulation; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000276:immunology; D007249:Inflammation / Q000517:prevention & control; D016899:Interferon-beta / Q000235:genetics / Q000276:immunology; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000276:immunology; D000072000:NF-KappaB Inhibitor alpha / Q000235:genetics / Q000276:immunology; D016328:NF-kappa B / Q000235:genetics* / Q000276:immunology; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000276:immunology; D010766:Phosphorylation; D050794:STAT1 Transcription Factor / Q000235:genetics* / Q000276:immunology; D015398:Signal Transduction; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D005419:Flavonoids; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D051267:NF-E2-Related Factor 2; D016328:NF-kappa B; C495636:Nfe2l2 protein, mouse; D050794:STAT1 Transcription Factor; C494080:Stat1 protein, mouse; D014409:Tumor Necrosis Factor-alpha; D000072000:NF-KappaB Inhibitor alpha; D009569:Nitric Oxide; C040015:myricetin; D016899:Interferon-beta; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse
keywords: HO-1; NF-κB; STAT1; anti-inflammation; myricetin