title: Porous dressings of modified chitosan with poly(2-hydroxyethyl acrylate) for topical wound delivery of levofloxacin.
abstract: Absorbable and non-absorbable dressings have been fabricated into sponges via a modified thermally induced phase separation method, using a grafted derivative of chitosan with 2-hydroxyethylacrylate (CS-g-PHEA). The material was synthesized via free-radical polymerization and was characterized with FT-IR and (1)H NMR spectroscopies. The swelling ability, biocompatibility and biodegradability of the dressings were evaluated through in vitro assays while antibacterial studies were performed using three different bacterial strains, Methicillin susceptible Staphylococcus aureus (MSSA), Methicillin resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa. Levofloxacin was used as model drug at different concentrations. Morphological characterization of the drug loaded dressings was performed by scanning electron microscopy, while drug-matrix interactions were evaluated by FT-IR spectroscopy. X-ray diffraction studies were carried out for the identification of the physical state for both neat and drug loaded materials. The prepared dressings showed a significant inhibition zone of the bacteria indicating the antibacterial property of the materials and loaded sponges.
authors.affiliation: Laboratory of Polymer Chemistry and Technology, Department of Chemistry, Aristotle University of Thessaloniki, Thessaloniki GR-541 24, Macedonia, Greece.
authors.forename: Panoraia I
authors.initials: PI
authors.lastname: Siafaka
authors.affiliation: Laboratory of Microbiology, Department of Medicine, Aristotle University of Thessaloniki, Thessaloniki GR-541 24, Macedonia, Greece.
authors.forename: Asimina P
authors.initials: AP
authors.lastname: Zisi
authors.affiliation: Laboratory of Microbiology, Department of Medicine, Aristotle University of Thessaloniki, Thessaloniki GR-541 24, Macedonia, Greece.
authors.forename: Maria K
authors.initials: MK
authors.lastname: Exindari
authors.affiliation: Ippokratio General Hospital, B Clinic of Internal Medicine, Thessaloniki GR-541 24, Macedonia, Greece.
authors.forename: Ioannis D
authors.initials: ID
authors.lastname: Karantas
authors.affiliation: Laboratory of Polymer Chemistry and Technology, Department of Chemistry, Aristotle University of Thessaloniki, Thessaloniki GR-541 24, Macedonia, Greece. Electronic address: dbic@chem.auth.gr.
authors.forename: Dimitrios N
authors.initials: DN
authors.lastname: Bikiaris
mesh_terms: D000179:Acrylates / Q000737:chemistry; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry; D001458:Bandages; D001672:Biocompatible Materials; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry; D016503:Drug Delivery Systems; D064704:Levofloxacin / Q000008:administration & dosage* / Q000737:chemistry; D051379:Mice; D009113:Muramidase / Q000737:chemistry; D016062:Porosity; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012972:Sodium Hydroxide / Q000737:chemistry; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects; D014961:X-Ray Diffraction
chemical_list: D000179:Acrylates; D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; C035957:2-hydroxyethyl acrylate; D012972:Sodium Hydroxide; D064704:Levofloxacin; D048271:Chitosan; D009113:Muramidase
keywords: Antibacterial; Chitosan; Derivatives; Dressing; Drug delivery; Levofloxacin