title: SCHISTOSOMICIDAL ACTIVITY OF THE CRUDE EXTRACT OF ARTOCARPUS LAKOOCHA.
abstract: Puag-Haad is a traditional anthelmintic drug used to treat taeniasis in Thailand and Lao PDR. It is derived from the aqueous extract of the plant Artocarpus lakoocha. We investigated the in vitro anthelmintic properties of Puag-Haad against Schistosoma mansoni. Adult worms were incubated in M-199 medium containing 250, 500 and 750 μg/ml of Puag-Haad or praziquantel (PZQ) at a concentration of 175 μg/ml for 3, 6, 12 and 24 hours. The relative motility (RM value), survival index (SI) and tegument alterations seen under scanning electron microscope were assessed at each incubation time. The results showed the crude extract of A. lakoocha at a concentration of 250 μg/ml was more effective in causing damage than PZQ at a concentration of 175 μg/ml using RM and SI values. The major target organ affected by Puag-Haad was the tegument. The damage was greater at higher concentrations of the crude extract. It is likely tetrahydroxystilbene (THS), the main compound in Puag-Haad, caused the damage. THS could be a future candidate as a schistosomal drug. Further studies are needed to explore its mechanism, efficiency and safety in vivo.
authors.forename: Narin
authors.initials: N
authors.lastname: Preyavichyapugdee
authors.forename: Manaw
authors.initials: M
authors.lastname: Sangfuang
authors.forename: Saowapark
authors.initials: S
authors.lastname: Chaiyapum
authors.forename: Suwatcharaporn
authors.initials: S
authors.lastname: Sriburin
authors.forename: Yupa
authors.initials: Y
authors.lastname: Pootaeng-on
authors.forename: Phiraphol
authors.initials: P
authors.lastname: Chusongsang
authors.forename: Wannee
authors.initials: W
authors.lastname: Jiraungkoorskul
authors.forename: Mananya
authors.initials: M
authors.lastname: Preyavichyapugdee
authors.forename: Prasert
authors.initials: P
authors.lastname: Sobhon
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology* / Q000627:therapeutic use; D007812:Laos; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D011223:Praziquantel / Q000627:therapeutic use; D012550:Schistosoma mansoni / Q000187:drug effects*; D012555:Schistosomiasis mansoni / Q000188:drug therapy* / Q000517:prevention & control; D013267:Stilbenes / Q000494:pharmacology* / Q000627:therapeutic use; D013622:Taeniasis / Q000188:drug therapy; D013785:Thailand
chemical_list: D000871:Anthelmintics; D010936:Plant Extracts; D013267:Stilbenes; C034912:puag-haad; D011223:Praziquantel