title: Histological validation of cardiac magnetic resonance T1mapping for detecting diffuse myocardial fibrosis in diabetic rabbits.
abstract: To pathologically verify the correlation between native T1mapping, postcontrast T1mapping, and extracellular volume fraction (ECV) and myocardial diffuse fibrosis, as determined by collagen volume fraction (CVF).
  
  
  
  Thirty New Zealand white rabbits were randomly divided into the control group (n = 6), diabetes 3 months group (n = 8), diabetes 6 months group (n = 8), and diabetes 9 months group (n = 8). All the rabbits underwent clinical 3.0T magnetic resonance (MR) examinations with pre- and postcontrast modified Look-Locker inversion recovery T1mapping. For the histological study, each rabbit was sacrificed after MR scanning, hematoxylin and eosin and Masson staining of the left ventricular myocardium were performed, and CVF was calculated. Pre- and postcontrast T1values and ECV were compared to CVF using Pearson's correlation coefficients.
  
  
  
  Two rabbits died in each diabetes group, thus each group included six rabbits. ECV calculated from pre- and postcontrast T1values showed a very strong correlation with CVF (r = 0.876, P < 0.001), whereas postcontrast T1values exhibited a moderate correlation with CVF (r = -0.564, P = 0.004). In contrast, precontrast T1values showed no correlation with CVF (r = 0.311, P = 0.139).
  
  
  
  ECV has a very strong correlation with pathological CVF, and can be used to assess the degree of diffuse myocardial fibrosis better than the postcontrast T1value. Precontrast T1value has no significant correlation with diffuse myocardial fibrosis. J. Magn. Reson. Imaging 2016;44:1179-1185.
authors.affiliation: Department of Radiology, Beijing An Zhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Disease, Beijing, China.
authors.forename: Mu
authors.initials: M
authors.lastname: Zeng
authors.affiliation: Department of Radiology, Beijing An Zhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Disease, Beijing, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Department of Radiology, Beijing An Zhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Disease, Beijing, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Radiology, Beijing An Zhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Disease, Beijing, China.
authors.forename: Zhaoying
authors.initials: Z
authors.lastname: Wen
authors.affiliation: Department of Radiology, Beijing An Zhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Disease, Beijing, China.
authors.forename: Zhanhong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Radiology, Beijing An Zhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Disease, Beijing, China.
authors.forename: Yike
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Siemens AG Healthcare Sector, Erlangen, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Greiser
authors.affiliation: MR Collaborations NE Asia, Siemens Healthcare, Beijing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: An
authors.affiliation: MR Collaborations NE Asia, Siemens Healthcare, Beijing, China.
authors.forename: Tianjing
authors.initials: T
authors.lastname: Zhang
authors.affiliation: School of Biomedical Engineering, Capital Medical University, Beijing, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Jing
authors.affiliation: National Key Laboratory of Biochemical Engineering Institute of Process Engineering, Chinese Academy of Sciences, Beijing, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Radiology, Beijing An Zhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Disease, Beijing, China. fanzm120@126.com.
authors.forename: Zhanming
authors.initials: Z
authors.lastname: Fan
authors.affiliation: Biomedical Imaging Research Institute, Cedars-Sinai Medical Center, Los Angeles, California, USA.
authors.forename: Debiao
authors.initials: D
authors.lastname: Li
mesh_terms: D000818:Animals; D057791:Cardiac Imaging Techniques / Q000379:methods*; D048909:Diabetes Complications / Q000000981:diagnostic imaging* / Q000473:pathology*; D005355:Fibrosis; D006321:Heart / Q000000981:diagnostic imaging*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009206:Myocardium / Q000473:pathology*; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
keywords: ECV; T1 mapping; diabetic rabbit; diffuse myocardial fibrosis; native T1 value; postcontrast T1 value