title: Prostaglandin E2 elevates calcium in differentiated neuroectodermal stem cells.
abstract: Lipid mediator prostaglandin E2 (PGE2) is an endogenous signaling molecule that plays an important role during early development of the nervous system. Abnormalities in the PGE2 signaling pathway have been associated with neurodevelopmental disorders such as autism spectrum disorders. In this study we use ratiometric fura-2AM calcium imaging to show that higher levels of PGE2 elevate intracellular calcium levels in the cell soma and growth cones of differentiated neuroectodermal (NE-4C) stem cells. PGE2 also increased the amplitude of calcium fluctuation in the neuronal growth cones and affected the neurite extension length. In summary, our results show that PGE2 may adversely impact intracellular calcium dynamics in differentiated neuronal cells and possibly affect early development of the nervous system.
authors.affiliation: Neuroscience Graduate Diploma Program, York University, Toronto, ON M3J 1P3, Canada; Department of Biology, York University, Toronto, ON M3J 1P3, Canada.
authors.forename: Jennilee M
authors.initials: JM
authors.lastname: Davidson
authors.affiliation: School of Kinesiology and Health Science, York University, Toronto, ON M3J 1P3, Canada; Neuroscience Graduate Diploma Program, York University, Toronto, ON M3J 1P3, Canada.
authors.forename: Christine T
authors.initials: CT
authors.lastname: Wong
authors.affiliation: Neuroscience Graduate Diploma Program, York University, Toronto, ON M3J 1P3, Canada; Department of Biology, York University, Toronto, ON M3J 1P3, Canada.
authors.forename: Ravneet
authors.initials: R
authors.lastname: Rai-Bhogal
authors.affiliation: School of Kinesiology and Health Science, York University, Toronto, ON M3J 1P3, Canada.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Kinesiology and Health Science, York University, Toronto, ON M3J 1P3, Canada; Neuroscience Graduate Diploma Program, York University, Toronto, ON M3J 1P3, Canada; Department of Biology, York University, Toronto, ON M3J 1P3, Canada. Electronic address: dakc@yorku.ca.
authors.forename: Dorota A
authors.initials: DA
authors.lastname: Crawford
mesh_terms: D000818:Animals; D020013:Calcium Signaling; D002478:Cells, Cultured; D015232:Dinoprostone / Q000494:pharmacology*; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D020439:Growth Cones / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D054258:Neural Plate / Q000166:cytology; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000071446:Neuronal Outgrowth
chemical_list: D015232:Dinoprostone
keywords: Autism spectrum disorders; Calcium fluctuations; Differentiated neuroectodermal stem cells; Growth cone; Neurite extension; Neurons; Prostaglandin E2