title: A molluscan calreticulin ortholog from Haliotis discus discus: Molecular characterization and transcriptional evidence for its role in host immunity.
abstract: Calreticulin (CALR), a Ca(2+) binding chaperone of the endoplasmic reticulum (ER) and mainly involved in Ca(2+) storage and signaling. In this study, we report the molecular characterization and immune responses of CALR homolog from disk abalone (AbCALR). The full length AbCALR cDNA (1837 bp) had an ORF of 1224 bp. According to the multiple alignments analysis, N- and P-domains were highly conserved in all the selected members of CALRs. In contrast, the C-domain which terminated with the characteristic ER retrieval signal (HDEL) was relatively less conserved. The phylogenetic analysis showed that all the selected molluscan homologs clustered together. Genomic sequence of AbCALR revealed that cDNA sequence was dispersed into ten exons interconnected with nine introns. AbCALR mRNA expression shows the significant (P < 0.05) up-regulation of AbCALR transcripts in hemocytes upon bacterial (Listeria monocytogenes and Vibrio parahaemolyticus), viral (Viral haemorrhagic septicaemia virus; VHSV) and immune stimulants (LPS and poly I:C) challenges at middle and/or late phases. These results collectively implied that AbCALR is able to be stimulated by pathogenic signals and might play a potential role in host immunity.
authors.affiliation: Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea; Fish Vaccine Research Center, Jeju National University, Jeju Special Self-Governing Province, 690-814, Republic of Korea.
authors.forename: H M V
authors.initials: HMV
authors.lastname: Udayantha
authors.affiliation: Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea; Fish Vaccine Research Center, Jeju National University, Jeju Special Self-Governing Province, 690-814, Republic of Korea.
authors.forename: G I
authors.initials: GI
authors.lastname: Godahewa
authors.affiliation: Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea; Fish Vaccine Research Center, Jeju National University, Jeju Special Self-Governing Province, 690-814, Republic of Korea.
authors.forename: S D N K
authors.initials: SDNK
authors.lastname: Bathige
authors.affiliation: Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea.
authors.forename: W D Niroshana
authors.initials: WDN
authors.lastname: Wickramaarachchi
authors.affiliation: Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea; Fish Vaccine Research Center, Jeju National University, Jeju Special Self-Governing Province, 690-814, Republic of Korea.
authors.forename: Navaneethaiyer
authors.initials: N
authors.lastname: Umasuthan
authors.affiliation: College of Veterinary Medicine, Chungnam National University, Yuseong-gu, Daejeon, 305-764, Republic of Korea.
authors.forename: Mahanama
authors.initials: M
authors.lastname: De Zoysa
authors.affiliation: Fish Vaccine Research Center, Jeju National University, Jeju Special Self-Governing Province, 690-814, Republic of Korea.
authors.forename: Hyung-Bok
authors.initials: HB
authors.lastname: Jeong
authors.affiliation: Fish Vaccine Research Center, Jeju National University, Jeju Special Self-Governing Province, 690-814, Republic of Korea. Electronic address: aidaro@naver.com.
authors.forename: Bong-Soo
authors.initials: BS
authors.lastname: Lim
authors.affiliation: Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea; Fish Vaccine Research Center, Jeju National University, Jeju Special Self-Governing Province, 690-814, Republic of Korea. Electronic address: jehee@jejunu.ac.kr.
authors.forename: Jehee
authors.initials: J
authors.lastname: Lee
mesh_terms: D000818:Animals; D037282:Calreticulin / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D016207:Cytokines / Q000276:immunology*; D007113:Immunity, Innate / Q000276:immunology*; D008974:Mollusca / Q000276:immunology*; D014157:Transcription Factors / Q000276:immunology*
chemical_list: D037282:Calreticulin; D016207:Cytokines; D014157:Transcription Factors
keywords: Bacterial and viral challenge; Calreticulin; Disk abalone; Genomic structure; In silco analysis