title: Bioactive, Elastic, and Biodegradable Emulsion Electrospun DegraPol Tube Delivering PDGF-BB for Tendon Rupture Repair.
abstract: Healing of tendon ruptures represents a major challenge in musculoskeletal injuries and combinations of biomaterials with biological factors are suggested as viable option for improved healing. The standard approach of repair by conventional suture leads to incomplete healing or rerupture. Here, a new elastic type of DegraPol® (DP), a polyester urethane, is explored as a delivery device for platelet-derived growth factor-BB (PDGF-BB) to promote tendon healing. Using emulsion electrospinning as an easy method for incorporation of biomolecules within polymers, DegraPol® supports loading and release of PDGF-BB. Morphological, mechanical and delivery device properties of the bioactive DP scaffolds, as well as differences arising due to different electrospinning parameters are studied. Emulsion electrospun DP scaffolds result in thinner fibers than pure DP scaffolds and experience decreased strain at break [%], but high enough for successful surgeon handling. PDGF-BB is released in a sustained manner from emulsion electrospun DP, but not completely, with still large amount of it being inside the polymeric fibers after 30 d. In vitro studies show that the bioactive scaffolds promote tenocyte proliferation in serum free and serum(+) conditions, demonstrating the potential of this surgeon-friendly bioactive delivery device to be used for tendon repair.
authors.affiliation: Division of Plastic Surgery and Hand Surgery, University Hospital Zürich, Sternwartstrasse 14, 8091, Zürich, Switzerland.|Laboratory of Applied Mechanobiology, ETH Zürich, Vladimir-Prelog-Weg 1-5/10, 8093, Zürich, Switzerland.
authors.forename: Olivera
authors.initials: O
authors.lastname: Evrova
authors.affiliation: Division of Plastic Surgery and Hand Surgery, University Hospital Zürich, Sternwartstrasse 14, 8091, Zürich, Switzerland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Houska
authors.affiliation: Division of Plastic Surgery and Hand Surgery, University Hospital Zürich, Sternwartstrasse 14, 8091, Zürich, Switzerland.
authors.forename: Manfred
authors.initials: M
authors.lastname: Welti
authors.affiliation: ab medica, Via J. F. Kennedy 10/12, 20023, Cerro Maggiore (Milan), Italy.
authors.forename: Eliana
authors.initials: E
authors.lastname: Bonavoglia
authors.affiliation: Division of Plastic Surgery and Hand Surgery, University Hospital Zürich, Sternwartstrasse 14, 8091, Zürich, Switzerland.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Calcagni
authors.affiliation: Division of Plastic Surgery and Hand Surgery, University Hospital Zürich, Sternwartstrasse 14, 8091, Zürich, Switzerland.
authors.forename: Pietro
authors.initials: P
authors.lastname: Giovanoli
authors.affiliation: Laboratory of Applied Mechanobiology, ETH Zürich, Vladimir-Prelog-Weg 1-5/10, 8093, Zürich, Switzerland.
authors.forename: Viola
authors.initials: V
authors.lastname: Vogel
authors.affiliation: Division of Plastic Surgery and Hand Surgery, University Hospital Zürich, Sternwartstrasse 14, 8091, Zürich, Switzerland.
authors.forename: Johanna
authors.initials: J
authors.lastname: Buschmann
mesh_terms: D000077214:Becaplermin; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000737:chemistry; D004655:Emulsions / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D011091:Polyesters / Q000008:administration & dosage* / Q000737:chemistry; D011140:Polyurethanes / Q000008:administration & dosage*; D020574:Proto-Oncogene Proteins c-sis / Q000008:administration & dosage* / Q000737:chemistry; D019651:Plastic Surgery Procedures; D012421:Rupture / Q000188:drug therapy* / Q000503:physiopathology / Q000601:surgery; D013708:Tendon Injuries / Q000188:drug therapy* / Q000503:physiopathology / Q000601:surgery; D013710:Tendons / Q000503:physiopathology / Q000601:surgery; D014945:Wound Healing / Q000187:drug effects
chemical_list: D001672:Biocompatible Materials; C104154:DegraPol; D004655:Emulsions; D011091:Polyesters; D011140:Polyurethanes; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin
keywords: PDGF-BB release; block copolymers; drug delivery systems; emulsion electrospining; rabbit tenocytes