title: Two legume defense proteins suppress the mobility of nasopharyngeal carcinoma cells.
abstract: A 16-kDa trypsin inhibitor was isolated from an edible legume using various chromatographic procedures. The protein was unadsorbed on Affi-gel blue gel but adsorbed on DEAE-Sepharose and Mono Q following which media the protein was subsequently subjected to gel filtration on Superdex 75 and a final 21-fold purification was achieved. This trypsin inhibitor showed remarkable pH and thermal stability. Its inhibitory activity was impaired in the presence of 1 mM dithiothreitol. The anti-proliferative and anti-mobility activities of this trypsin inhibitor and a hemagglutinin isolated from the same legume were tested on nasopharyngeal carcinoma cells. These two defense proteins demonstrated discrepant anti-proliferative efficacies that the hemagglutinin could greatly suppress the proliferation of nasopharyngeal carcinoma cells, while the trypsin inhibitor revealed a minor effect. However, these two proteins could both attenuate the mobility of nasopharyngeal carcinoma cells. The present study revealed the potential of applying plant defense proteins in cancer treatment.
authors.affiliation: a School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong , Hong Kong , P.R. China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Dan
authors.affiliation: a School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong , Hong Kong , P.R. China.
authors.forename: Tzi Bun
authors.initials: TB
authors.lastname: Ng
mesh_terms: D002277:Carcinoma; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000473:pathology*; D027805:Phaseolus / Q000737:chemistry*; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D014361:Trypsin Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D010940:Plant Proteins; D014361:Trypsin Inhibitors
keywords: Hemagglutinin; legume defense proteins; nasopharyngeal carcinoma cells; trypsin inhibitor