title: Enhanced lipid recovery from Nannochloropsis microalgae by treatment with optimized cell wall degrading enzyme mixtures.
abstract: A statistical mixture design approach was used to investigate the effects of cell wall degrading enzymes on the recovery of lipids from Nannochloropsis sp. A preliminary screening of potentially suitable enzyme preparations, including lysozyme, cellulase and different types of hemicellulases, was carried out. The most effective preparations were then taken as basic components for the formulation of enzyme mixtures. Optimized ternary mixtures consisting of cellulase and two hemicellulases were obtained which allowed the recovery of up to 37.2g of lipids per 100g of dry biomass. SEM and TEM images of the enzymatically treated microalga revealed extensive cell damage, with degradation of the cell wall and release of intracellular material. Overall, the results obtained demonstrate that the mixture design method can be used to prepare cell wall degrading enzyme cocktails that can significantly improve the recovery of lipids or other valuable components from microalgae.
authors.affiliation: Department of Chemical Engineering, Materials and Environment, Sapienza University, Roma, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Zuorro
authors.affiliation: Department of Anatomical, Histological, Forensic and Locomotor Apparatus Sciences, Sapienza University, Roma, Italy.
authors.forename: Selenia
authors.initials: S
authors.lastname: Miglietta
authors.affiliation: Department of Anatomical, Histological, Forensic and Locomotor Apparatus Sciences, Sapienza University, Roma, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Familiari
authors.affiliation: Department of Chemical Engineering, Materials and Environment, Sapienza University, Roma, Italy. Electronic address: roberto.lavecchia@uniroma1.it.
authors.forename: Roberto
authors.initials: R
authors.lastname: Lavecchia
mesh_terms: D018533:Biomass; D002473:Cell Wall / Q000378:metabolism / Q000648:ultrastructure; D002480:Cellulase / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000494:pharmacology; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis / Q000302:isolation & purification*; D058086:Microalgae / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D009113:Muramidase / Q000494:pharmacology; D058009:Stramenopiles / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D008055:Lipids; D006026:Glycoside Hydrolases; C023305:hemicellulase; D009113:Muramidase; D002480:Cellulase
keywords: Cell wall degrading enzymes; Lipid recovery; Mixture design; Nannochloropsis; Optimization