title: Selective dispersive solid phase extraction-chromatography tandem mass spectrometry based on aptamer-functionalized UiO-66-NH2 for determination of polychlorinated biphenyls.
abstract: In this paper, a novel dispersive solid phase extraction (dSPE) adsorbent based on aptamer-functionalized magnetic metal-organic framework material was developed for selective enrichment of the trace polychlorinated biphenyls (PCBs) from soil sample. Firstly, we developed a simple, versatile synthetic strategy to prepare highly reproducible magnetic amino-functionalized UiO-66 (Fe3O4@PDA@UiO-66-NH2) by using polydopamine (PDA) as covalent linker. Then amino-functionalized aptamers which can recognize 2,3',5,5'-tetrachlorobiphenyl (PCB72), 2',3',4',5,5'-pentachlorobiphenyl (PCB106) were covalent immobilized on UiO-66-NH2 through coupling reagent of glutaraldehyde. Aptamer-functionalized adsorbent (Fe3O4@PDA@UiO-66-Apt) can specifically capture PCBs from complex matrix with high adsorption capacity based on the specific affinity of aptamer towards target. Moreover, the adsorbent can be easily isolated from the solution through magnetic separation after extraction. Afterwards, the detection was carried out with gas chromatography tandem mass spectrometry (GC-MS). The selective dSPE pretreatment coupled with GC-MS possessed high selectivity, good binding capacity, stability, repeatability and reproducibility for the extraction of PCBs. Furthermore, the adsorbent possessed good mechanical stability which can be applied in replicate at least for 60 extraction cycles with recovery over 80%. It provided a linear range of 0.02-400ngmL(-1) with a good correlation coefficient (R(2)=0.9994-0.9996), and the limit of detection was found to be 0.010-0.015ngmL(-1). The method was successfully utilized for the determination of PCBs in soil samples.
authors.affiliation: The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China.
authors.forename: Saichai
authors.initials: S
authors.lastname: Lin
authors.affiliation: The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China. Electronic address: ganning@nbu.edu.cn.
authors.forename: Ning
authors.initials: N
authors.lastname: Gan
authors.affiliation: The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China. Electronic address: caoyuting@nbu.edu.cn.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Deptartment of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, Nanjing 210007, China.
authors.forename: Yinji
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Qianli
authors.initials: Q
authors.lastname: Jiang
mesh_terms: D000327:Adsorption; D052157:Aptamers, Nucleotide; D002712:Chlorides; D008401:Gas Chromatography-Mass Spectrometry; D007211:Indoles; D058185:Magnetite Nanoparticles; D009942:Organometallic Compounds; D011078:Polychlorinated Biphenyls / Q000032:analysis*; D011108:Polymers; D015203:Reproducibility of Results; D012989:Soil Pollutants / Q000032:analysis*; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry; D015040:Zirconium
chemical_list: D052157:Aptamers, Nucleotide; D002712:Chlorides; D007211:Indoles; D058185:Magnetite Nanoparticles; D009942:Organometallic Compounds; D011108:Polymers; D012989:Soil Pollutants; C568283:polydopamine; D015040:Zirconium; D011078:Polychlorinated Biphenyls; C429984:zirconium chloride
keywords: Aptamer; Dispersive solid phase extraction; GC–MS; Metal-organic frameworks; Polychlorinated biphenyls