title: An Eighteen-Gene Classifier Predicts Locoregional Recurrence in Post-Mastectomy Breast Cancer Patients.
abstract: We previously identified 34 genes of interest (GOI) in 2006 to aid the oncologists to determine whether post-mastectomy radiotherapy (PMRT) is indicated for certain patients with breast cancer. At this time, an independent cohort of 135 patients having DNA microarray study available from the primary tumor tissue samples was chosen. Inclusion criteria were 1) mastectomy as the first treatment, 2) pathology stages I-III, 3) any locoregional recurrence (LRR) and 4) no PMRT. After inter-platform data integration of Affymetrix U95 and U133 Plus 2.0 arrays and quantile normalization, in this paper we used 18 of 34 GOI to divide the mastectomy patients into high and low risk groups. The 5-year rate of freedom from LRR in the high-risk group was 30%. In contrast, in the low-risk group it was 99% (p < 0.0001). Multivariate analysis revealed that the 18-gene classifier independently predicts rates of LRR regardless of nodal status or cancer subtype.
authors.affiliation: Koo Foundation Sun Yat-Sen Cancer Center, Taipei, Taiwan.
authors.forename: Skye H
authors.initials: SH
authors.lastname: Cheng
authors.affiliation: Koo Foundation Sun Yat-Sen Cancer Center, Taipei, Taiwan.
authors.forename: Chen-Fang
authors.initials: CF
authors.lastname: Horng
authors.affiliation: Koo Foundation Sun Yat-Sen Cancer Center, Taipei, Taiwan.
authors.forename: Tzu-Ting
authors.initials: TT
authors.lastname: Huang
authors.affiliation: Duke University Medical Center, Durham, NC, USA.
authors.forename: Erich S
authors.initials: ES
authors.lastname: Huang
authors.affiliation: Koo Foundation Sun Yat-Sen Cancer Center, Taipei, Taiwan.
authors.forename: Mei-Hua
authors.initials: MH
authors.lastname: Tsou
authors.affiliation: Department of Laboratory and Pathology, Koo Foundation Sun Yat-Sen Cancer Center, Taipei, Taiwan.
authors.forename: Li-Sun
authors.initials: LS
authors.lastname: Shi
authors.affiliation: Department of Surgery, Koo Foundation Sun Yat-Sen Cancer Center, Taipei, Taiwan.
authors.forename: Ben-Long
authors.initials: BL
authors.lastname: Yu
authors.affiliation: Koo Foundation Sun Yat-Sen Cancer Center, Taipei, Taiwan.
authors.forename: Chii-Ming
authors.initials: CM
authors.lastname: Chen
authors.affiliation: Koo Foundation Sun Yat-Sen Cancer Center, Taipei, Taiwan; Duke University, USA.
authors.forename: Andrew T
authors.initials: AT
authors.lastname: Huang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008408:Mastectomy; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics*; D009364:Neoplasm Recurrence, Local / Q000235:genetics* / Q000473:pathology; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D059467:Transcriptome
chemical_list: D009363:Neoplasm Proteins
keywords: Breast cancer; Breast-conserving surgery; Gene-expression profiles; Locoregional recurrence; Mastectomy; Prediction; Radiotherapy