title: Comparative proteomic analysis of CD34(+) cells in bone marrow between severe aplastic anemia and normal control.
abstract: Severe aplastic anemia (SAA) is an autoimmune disease with destruction of hematopoietic cells by activated T lymphocytes. However, the precise mechanism of cytotoxicity T cells recognizing and attacking CD34(+) cells remains unclear. Here, we investigated the proteome of CD34(+) cells in SAA patients to further explore the pathogenesis of SAA. CD34(+) cells from 29 SAA patients and 20 health controls were isolated by magnetic activated cell sorting. The protein of CD34(+) cells were examined by iTRAQ labeling combination of multidimensional liquid chromatography and tandem mass spectrometry. A total of 156 differential expression proteins in CD34(+) cells were identified. Compared with health controls, 53 proteins were up-regulated and 103 proteins were down-regulated in SAA patients. Specifically, abnormal expression of proteasome subunits, histone variants, dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit (DAD1) and ATPase inhibitor, mitochondrial isoform 1 precursor(IF1) may relate to the hyperfunction of immune responses and excessive apoptosis of SAA CD34(+) cells.
authors.affiliation: Department of Hematology, The General Hospital of Tianjin Medical University, Tianjin 300052, PR China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Qi
authors.affiliation: Department of Hematology, The General Hospital of Tianjin Medical University, Tianjin 300052, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Fu
authors.affiliation: Department of Hematology, The General Hospital of Tianjin Medical University, Tianjin 300052, PR China.
authors.forename: Huaquan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Hematology, The General Hospital of Tianjin Medical University, Tianjin 300052, PR China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Hematology, The General Hospital of Tianjin Medical University, Tianjin 300052, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Department of Hematology, The General Hospital of Tianjin Medical University, Tianjin 300052, PR China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Shao
authors.affiliation: Department of Hematology, The General Hospital of Tianjin Medical University, Tianjin 300052, PR China. Electronic address: shaozonghong@sina.com.
authors.forename: Zonghong
authors.initials: Z
authors.lastname: Shao
mesh_terms: D000293:Adolescent; D000328:Adult; D000741:Anemia, Aplastic / Q000378:metabolism*; D018952:Antigens, CD34 / Q000378:metabolism; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000378:metabolism* / Q000473:pathology; D002648:Child; D018450:Disease Progression; D005260:Female; D006657:Histones / Q000378:metabolism; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D020543:Proteome; D053719:Tandem Mass Spectrometry; D055815:Young Adult; D000097795:ATPase Inhibitory Protein
chemical_list: D018952:Antigens, CD34; D051017:Apoptosis Regulatory Proteins; D006657:Histones; D008565:Membrane Proteins; D046988:Proteasome Endopeptidase Complex; D011506:Proteins; D020543:Proteome; D000097795:ATPase Inhibitory Protein; C083331:DAD1 protein, human
keywords: Aplastic anemia; CD34(+) cells; Proteome