title: Engineered Protein Polymer-Gold Nanoparticle Hybrid Materials for Small Molecule Delivery.
abstract: We have fabricated protein polymer-gold nanoparticle (P-GNP) nanocomposites that exhibit enhanced binding and delivery properties of the small hydrophobic molecule drug, curcumin, to the model breast cancer cell line, MCF-7. These hybrid biomaterials are constructed viain situGNP templated-synthesis with genetically engineered histidine tags. The P-GNP nanocomposites exhibit enhanced small molecule loading, sustained release and increased uptake by MCF-7 cells. When compared to the proteins polymers alone, the P-GNPs demonstrate a greater than 7-fold increase in curcumin binding, a nearly 50% slower release profile and more than 2-fold increase in cellular uptake of curcumin. These results suggest that P-GNP nanocomposites serve as promising candidates for drug delivery vehicles.
authors.affiliation: Department of Chemical and Biomolecular Engineering, NYU Tandon School of Engineering, Brooklyn, New York 11201, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Dai
authors.affiliation: Department of Chemical and Biomolecular Engineering, NYU Tandon School of Engineering, Brooklyn, New York 11201, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Frezzo
authors.affiliation: Department of Chemical and Biomolecular Engineering, NYU Tandon School of Engineering, Brooklyn, New York 11201, USA.
authors.forename: E
authors.initials: E
authors.lastname: Sharma
authors.affiliation: Department of Chemical and Biomolecular Engineering, NYU Tandon School of Engineering, Brooklyn, New York 11201, USA.
authors.forename: R
authors.initials: R
authors.lastname: Chen
authors.affiliation: Department of Chemical and Biomolecular Engineering, NYU Tandon School of Engineering, Brooklyn, New York 11201, USA.
authors.forename: N
authors.initials: N
authors.lastname: Singh
authors.affiliation: Department of Chemical and Biomolecular Engineering, NYU Tandon School of Engineering, Brooklyn, New York 11201, USA.
authors.forename: C
authors.initials: C
authors.lastname: Yuvienco
authors.affiliation: Department of Biology, Brooklyn College and Graduate Center, City University of New York, Brooklyn, New York 11210, USA.
authors.forename: E
authors.initials: E
authors.lastname: Caglar
authors.affiliation: Department of Biology, Brooklyn College and Graduate Center, City University of New York, Brooklyn, New York 11210, USA.
authors.forename: S
authors.initials: S
authors.lastname: Xiao
authors.affiliation: Department of Biology, Brooklyn College and Graduate Center, City University of New York, Brooklyn, New York 11210, USA.
authors.forename: A
authors.initials: A
authors.lastname: Saxena
authors.affiliation: Department of Chemical and Biomolecular Engineering, NYU Tandon School of Engineering, Brooklyn, New York 11201, USA; Department of Chemistry, New York University, New York, New York 10003, USA; Department of Biochemistry, SUNY Downstate Medical Center, Brooklyn, New York 11203, USA.
authors.forename: J K
authors.initials: JK
authors.lastname: Montclare
keywords: Drug delivery; Gold nanoparticles; Nanocomposites; Proteins