title: Important roles played by TGF-β member of Bmdpp and Bmdaw in BmNPV infection.
abstract: Transforming growth factor (TGF)-β superfamily members inhibit Bombyx mori nucleohedrovirus (BmNPV) multiplication in silkworm are not determined. In this study, we first found that BmNPV RNA transcription and protein expression level were regulated by TGF-β members, Decapentaplegic (Bmdpp) and Dawdle (Bmdaw) in the domesticated silkworm, B. mori and silkworm ovary-derived cells. Furthermore, subcellular localization showed that Bmdpp and Bmdaw were mainly presented in cytomembrane of the cultured BmN cells. Tissues expression pattern analysis found that the highest expression levels of Bmdpp and Bmdaw genes were in the hemocyte of fifth instar larvae. During the immune response, the expression level of Bmdpp gene was elevated and Bmdaw gene was declined in BmNPV infected BmN cells and silkworm. The multiplication of BmNPV was inhibited by overexpression of Bmdpp and Bmdaw genes in BmN cells. RNA interference experiments found that the multiplication of BmNPV was raised with specific siRNAs of Bmdpp and Bmdaw genes in BmN cells. The antiviral immune pathways were not significantly regulated by the TGF-β superfamily members. Taken together, these findings provided a clue to understand the function of Bmdpp and Bmdaw gene in response to the BmNPV infection in silkworm.
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China; National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Hu
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China.
authors.forename: Yongchang
authors.initials: Y
authors.lastname: Gong
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Zhu
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Chen
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China.
authors.forename: Zi
authors.initials: Z
authors.lastname: Liang
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China.
authors.forename: Sulan
authors.initials: S
authors.lastname: Kuang
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China.
authors.forename: Mian Sahib
authors.initials: MS
authors.lastname: Zar
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China.
authors.forename: Dhiraj
authors.initials: D
authors.lastname: Kumar
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China; National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, China.
authors.forename: Guangli
authors.initials: G
authors.lastname: Cao
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China; National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, China.
authors.forename: Renyu
authors.initials: R
authors.lastname: Xue
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China; National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, China. Electronic address: gongcl@suda.edu.cn.
authors.forename: Chengliang
authors.initials: C
authors.lastname: Gong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D012831:Bombyx / Q000235:genetics* / Q000276:immunology* / Q000821:virology; D005455:Fluorescent Antibody Technique; D017344:Genes, Insect / Q000235:genetics / Q000276:immunology; D007113:Immunity, Innate / Q000235:genetics / Q000276:immunology*; D019476:Insect Proteins / Q000235:genetics / Q000276:immunology*; D017924:Nucleopolyhedroviruses / Q000276:immunology; D060888:Real-Time Polymerase Chain Reaction; D017385:Sequence Homology; D016212:Transforming Growth Factor beta / Q000276:immunology*
chemical_list: D019476:Insect Proteins; D016212:Transforming Growth Factor beta
keywords: BmNPV; Bombyx mori; TGF-&beta; dawdle; decapentaplegic