title: A new microscopic method to analyse desiccation-induced volume changes in aeroterrestrial green algae.
abstract: Aeroterrestrial green algae are exposed to desiccation in their natural habitat, but their actual volume changes have not been investigated. Here, we measure the relative volume reduction (RVRED ) in Klebsormidium crenulatum and Zygnema sp. under different preset relative air humidities (RH). A new chamber allows monitoring RH during light microscopic observation of the desiccation process. The RHs were set in the range of ∼4 % to ∼95% in 10 steps. RVRED caused by the desiccation process was determined after full acclimation to the respective RHs. In K. crenulatum, RVRED (mean ± SE) was 46.4 ± 1.9%, in Zygnema sp. RVRED was only 34.3 ± 2.4% at the highest RH (∼95%) tested. This indicates a more pronounced water loss at higher RHs in K. crenulatum versus Zygnema sp. By contrast, at the lowest RH (∼4%) tested, RVRED ranged from 75.9 ± 2.7% in K. crenulatum to 83.9 ± 2.2% in Zygnema sp. The final volume reduction is therefore more drastic in Zygnema sp. These data contribute to our understanding of the desiccation process in streptophytic green algae, which are considered the closest ancestors of land plants.
authors.affiliation: University of Innsbruck, Institute of Botany, Functional Plant Biology, Sternwartestrasse 15, 6020, Innsbruck, Austria.|Present address: Szent István University, Plant Protection Institute, Páter Károly utca 1, H-2100, Gödöllő, Hungary.
authors.forename: K
authors.initials: K
authors.lastname: Lajos
authors.affiliation: University of Innsbruck, Institute of Botany, Functional Plant Biology, Sternwartestrasse 15, 6020, Innsbruck, Austria.
authors.forename: S
authors.initials: S
authors.lastname: Mayr
authors.affiliation: University of Innsbruck, Institute of Botany, Functional Plant Biology, Sternwartestrasse 15, 6020, Innsbruck, Austria.
authors.forename: O
authors.initials: O
authors.lastname: Buchner
authors.affiliation: University of Innsbruck, Institute of Botany, Functional Plant Biology, Sternwartestrasse 15, 6020, Innsbruck, Austria.
authors.forename: K
authors.initials: K
authors.lastname: Blaas
authors.affiliation: University of Innsbruck, Institute of Botany, Functional Plant Biology, Sternwartestrasse 15, 6020, Innsbruck, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Holzinger
mesh_terms: D000460:Chlorophyta / Q000166:cytology / Q000378:metabolism*; D003890:Desiccation; D006813:Humidity; D008853:Microscopy / Q000379:methods*; D057949:Streptophyta / Q000166:cytology / Q000378:metabolism*; D014867:Water / Q000032:analysis
chemical_list: D014867:Water
keywords: Desiccation; Streptophyta; hydraulic parameter; light microscopy; water loss