title: [Effects of PRF and released three growth factors on migration of rat adipose tissue-derived stem cells].
abstract: OBJECTIVE
  To analyze the effects of PRF and released three growth factors on migration of rat adipose tissue-derived stem cells and to investigate the mechanism of migration.
  
  
  METHODS
  The inguinal adipose tissue of rat was excised at aseptic condition to obtain primary ADSCs by enzyme digestion. Multi-directional differentiation was used to identify the ADSCs. PRF membrane was acquired through one time centrifuge. The cell migration was examined by Transwell assay and wound healing assay. The mRNA expression of MMP2 and MT1-MMP was tested by real-time PCR. Statistical analysis was performed using SPSS 13.0 software package.
  
  
  RESULTS
  Cell migration test showed that the migration of rat ADSCs in PRF group were significantly higher than those in the negative group（P<0.05） and inhibitor group（P<0.05）. The ADSCs migration effects in three growth factors group at different concentrations showed significant difference（P<0.05）. Real-time PCR showed that gene expressions of MMP2 and MT1-MMP were significantly higher in PRF group than control group (P<0.05). PCR showed that gene expressions of MMP2 and MT1-MMP were significantly higher in three growth factors group than control group (P<0.05).
  
  
  CONCLUSIONS
  PRF and three growth factors consistently enhanced the migration of rat ADSCs in a dose-response manner. The migration increase of rat ADSCs may be associated with the up-regulation of MMP2 and MT1-MMP gene expression.
authors.affiliation: Department of Stomatology, the 401st Hospital of Jinan Military Area, PLA.Qingdao 266071, China. E-mail:gaojierizhao@126.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Stomatology, Jinan Central Hospital. Jinan 250013, China.
authors.forename: Ming-guo
authors.initials: MG
authors.lastname: Wang
authors.affiliation: Department of Stomatology, Jinan Central Hospital. Jinan 250013, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Stomatology, Jinan Municipal People's Government. Jinan 250099, China.
authors.forename: Xiu-mei
authors.initials: XM
authors.lastname: Li
authors.affiliation: Department of Stomatology, Jinan Municipal People's Government. Jinan 250099, China.
authors.forename: Shi-mao
authors.initials: SM
authors.lastname: Yang
authors.affiliation: Hospital of Stomatology, Weifang Medical University. Weifang 261053, Shandong Province,China.
authors.forename: Xue
authors.initials: X
authors.lastname: Li
mesh_terms: D000273:Adipose Tissue; D000818:Animals; D002454:Cell Differentiation; D002465:Cell Movement; D005337:Fibrin / Q000627:therapeutic use*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D051381:Rats; D013234:Stem Cells; D054457:Tissue Scaffolds; D015854:Up-Regulation; D014945:Wound Healing / Q000502:physiology*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D005337:Fibrin; D020778:Matrix Metalloproteinase 2