title: Evaluation of immuno diagnostic assay for the exposure of stage specific filarial infection.
abstract: Lymphatic filariasis is a debilitating diseases caused by filarial parasitic nematodes. The infection may be acquired in childhood but the symptoms become apparent only in later life. To evaluate the success of any intervention, sensitive diagnostics were used to identify infection among endemic normals that are likely to develop microfilaremia in due course of time. Capture assay was standardized using the recombinant protein Brugia malayi Abundant Larval Transcript-2 (ALT-2) specific monoclonal and poly-clonal antibodies and evaluated with serum samples of clinical groups from high and low filarial infection area individuals (HIA/LIA), Endemic Normal (EN, n = 478), microfilaeremics (MF, n = 77), chronic pathology (CP, n = 57) and non endemic normal (NEN, n = 20). In order to assess stage-specific infection, ALT-2 capture assay was compared with the early reported Venom allergen homologue (VAH) and microfilariae specific SXP-1 capture assays. Of the 632 serum samples tested, ALT-2 and VAH capture assays detected circulating filarial antigen (CFA) in 57% and 52% of HIA-EN individuals, respectively. As expected, the VAH and SXP-1 capture assays were positive for 100 % of MF individuals. The described capture assays can be useful for the detection of early and stage-specific filarial infections in endemic regions of developing countries.
authors.forename: Rajendran
authors.initials: R
authors.lastname: Ravishankaran
authors.forename: Radhika Nagamangalam
authors.initials: RN
authors.lastname: Shridharan
authors.forename: Lawrence Ansel
authors.initials: LA
authors.lastname: Vishal
authors.forename: Sankaranarayanan
authors.initials: S
authors.lastname: Meenakshisundaram
authors.forename: Anjali Anoop
authors.initials: AA
authors.lastname: Karande
authors.forename: Perumal
authors.initials: P
authors.lastname: Kaliraj
mesh_terms: D000909:Antibodies, Helminth / Q000276:immunology; D000947:Antigens, Helminth / Q000097:blood*; D003955:Diagnostic Tests, Routine / Q000379:methods*; D004605:Elephantiasis, Filarial / Q000175:diagnosis*; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008297:Male; D012680:Sensitivity and Specificity
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth