title: ANXA2 enhances the progression of hepatocellular carcinoma via remodeling the cell motility associated structures.
abstract: Hepatocellular carcinoma (HCC) ranks as the fifth most common malignancy worldwide. The detailed mechanism of signal regulation for HCC progression is still not known, and the high motility of cancer cells is known as a core property for cancer progression maintenance. Annexin A2 (ANXA2), a calcium-dependent phospholipids binding protein is highly expressed in HCC. To study the roles the excessively expressed ANXA2 during the progression of HCC, we inhibited the ANXA2 expression in SMMC-7721 cells using RNAi, followed by the analysis of cell growth, apoptosis and cell motility. To explore the relationship between the cell behaviors and its structures, the microstructure changes were observed under fluorescence microscopy, laser scanning confocal microscopy and electron microscopy. Our findings demonstrated that down-regulation of ANXA2 results in decreased the cell proliferation and motility, enhanced apoptosis, suppressed cell pseudopodia/filopodia, inhibited expression of F-actin and β-tubulin, and inhibited or depolymerized Lamin B. The cell contact inhibition was also analyzed in the paper. Take together, our results indicate that ANXA2 plays an important role to enhance the malignant behaviors of HCC cells, and the enhancement is closely based on its remodeling to cell structures.
authors.affiliation: Co-Innovation Center for Qinba Region's Sustainable Development, Shaanxi Normal University, Xi'an, Shaanxi 710062, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Shi
authors.affiliation: Co-Innovation Center for Qinba Region's Sustainable Development, Shaanxi Normal University, Xi'an, Shaanxi 710062, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xiao
authors.affiliation: School of Medicine, Deakin University, Waurn Ponds, VIC 3216, Australia.
authors.forename: Wei
authors.initials: W
authors.lastname: Duan
authors.affiliation: Co-Innovation Center for Qinba Region's Sustainable Development, Shaanxi Normal University, Xi'an, Shaanxi 710062, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: He
authors.affiliation: Co-Innovation Center for Qinba Region's Sustainable Development, Shaanxi Normal University, Xi'an, Shaanxi 710062, China.
authors.forename: Lele
authors.initials: L
authors.lastname: Ma
authors.affiliation: Co-Innovation Center for Qinba Region's Sustainable Development, Shaanxi Normal University, Xi'an, Shaanxi 710062, China.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Da
authors.affiliation: Co-Innovation Center for Qinba Region's Sustainable Development, Shaanxi Normal University, Xi'an, Shaanxi 710062, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Duan
authors.affiliation: Co-Innovation Center for Qinba Region's Sustainable Development, Shaanxi Normal University, Xi'an, Shaanxi 710062, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: The High School Affiliated to Shaanxi Normal University, Xi'an, Shaanxi 710061, China.
authors.forename: Huayi
authors.initials: H
authors.lastname: Wu
authors.affiliation: The High School Affiliated to Shaanxi Normal University, Xi'an, Shaanxi 710061, China.
authors.forename: Xigui
authors.initials: X
authors.lastname: Song
authors.affiliation: Co-Innovation Center for Qinba Region's Sustainable Development, Shaanxi Normal University, Xi'an, Shaanxi 710062, China. Electronic address: ychhou@snnu.edu.cn.
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Hou
mesh_terms: D008841:Actin Cytoskeleton / Q000235:genetics / Q000648:ultrastructure; D000199:Actins / Q000235:genetics / Q000378:metabolism; D017306:Annexin A2 / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation / Q000235:genetics; D018450:Disease Progression; D015870:Gene Expression; D006801:Humans; D034921:Lamin Type B / Q000378:metabolism; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology*; D011554:Pseudopodia / Q000648:ultrastructure; D034622:RNA Interference; D014404:Tubulin / Q000235:genetics / Q000378:metabolism
chemical_list: C472822:ANXA2 protein, human; D000199:Actins; D017306:Annexin A2; D034921:Lamin Type B; D014404:Tubulin
keywords: ANXA2; Cell behaviors; Cell structures; Gene expression; Hepatocellular carcinoma