title: Preeclampsia/Eclampsia candidate genes show altered methylation in maternal leukocytes of preeclamptic women at the time of delivery.
abstract: OBJECTIVE
  To analyze methylation profiles of known preeclampsia/eclampsia (PE) candidate genes in normal (NL) and preeclamptic (PE) women at delivery.
  
  
  METHODS
  A matched case-control study comparing methylation in 79 CpG sites/33 genes from an independent gene set in maternal leukocyte DNA in PE and NL (n = 14 each) on an Illumina BeadChip platform. Replication performed on second cohort (PE = 12; NL = 32).
  
  
  RESULTS
  PE demonstrates differential methylation in POMC, AGT, CALCA, and DDAH1 compared with NL.
  
  
  CONCLUSIONS
  Differential methylation in four genes associated with PE may represent a potential biomarker or an epigenetic pathophysiologic mechanism altering gene transcription.
authors.affiliation: a Department of Obstetrics and Gynecology , Division of Maternal Fetal Medicine , Mayo Clinic, Rochester , MN , USA.
authors.forename: Wendy M
authors.initials: WM
authors.lastname: White
authors.affiliation: b Department of Health Sciences Research , Division of Biomedical Statistics and Informatics , Mayo Clinic, Rochester , MN , USA.
authors.forename: Zhifu
authors.initials: Z
authors.lastname: Sun
authors.affiliation: a Department of Obstetrics and Gynecology , Division of Maternal Fetal Medicine , Mayo Clinic, Rochester , MN , USA.
authors.forename: Kristi S
authors.initials: KS
authors.lastname: Borowski
authors.affiliation: a Department of Obstetrics and Gynecology , Division of Maternal Fetal Medicine , Mayo Clinic, Rochester , MN , USA.
authors.forename: Brian C
authors.initials: BC
authors.lastname: Brost
authors.affiliation: a Department of Obstetrics and Gynecology , Division of Maternal Fetal Medicine , Mayo Clinic, Rochester , MN , USA.
authors.forename: Norman P
authors.initials: NP
authors.lastname: Davies
authors.affiliation: a Department of Obstetrics and Gynecology , Division of Maternal Fetal Medicine , Mayo Clinic, Rochester , MN , USA.
authors.forename: Carl H
authors.initials: CH
authors.lastname: Rose
authors.affiliation: c Department of Internal Medicine , Division of Nephrology and Hypertension , Mayo Clinic, Rochester , MN , USA.
authors.forename: Vesna D
authors.initials: VD
authors.lastname: Garovic
mesh_terms: D000328:Adult; D000581:Amidohydrolases / Q000235:genetics / Q000378:metabolism; D000808:Angiotensinogen / Q000235:genetics / Q000378:metabolism; D015740:Calcitonin Gene-Related Peptide / Q000235:genetics / Q000378:metabolism; D016022:Case-Control Studies; D018899:CpG Islands; D019175:DNA Methylation; D004461:Eclampsia / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D036801:Parturition / Q000502:physiology*; D011225:Pre-Eclampsia / Q000235:genetics* / Q000378:metabolism; D011247:Pregnancy; D011333:Pro-Opiomelanocortin / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: C534931:AGT protein, human; C000606589:CALCA protein, human; D000808:Angiotensinogen; D011333:Pro-Opiomelanocortin; D000581:Amidohydrolases; C059999:dimethylargininase; D015740:Calcitonin Gene-Related Peptide
keywords: AGT; CALCA; DDAH1; DNA methylation; POMC; candidate gene; leukocyte; preeclampsia; pregnancy