title: Single-Molecule Studies in Live Cells.
abstract: Live-cell single-molecule experiments are now widely used to study complex biological processes such as signal transduction, self-assembly, active trafficking, and gene regulation. These experiments' increased popularity results in part from rapid methodological developments that have significantly lowered the technical barriers to performing them. Another important advance is the development of novel statistical algorithms, which, by modeling the stochastic behaviors of single molecules, can be used to extract systemic parameters describing the in vivo biochemistry or super-resolution localization of biological molecules within their physiological environment. This review discusses recent advances in experimental and computational strategies for live-cell single-molecule studies, as well as a selected subset of biological studies that have utilized these new technologies.
authors.affiliation: Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, Connecticut 06030; email: jyu@uchc.edu.
authors.forename: Ji
authors.initials: J
authors.lastname: Yu
mesh_terms: D000818:Animals; D002470:Cell Survival; D049229:Dendritic Spines / Q000378:metabolism; D004058:Diffusion; D006801:Humans; D008390:Markov Chains; D008856:Microscopy, Fluorescence / Q000379:methods*; D008954:Models, Biological; D015233:Models, Statistical; D009707:Nucleosomes / Q000378:metabolism; D061848:Optical Imaging / Q000379:methods*; D015398:Signal Transduction; D013269:Stochastic Processes
chemical_list: D009707:Nucleosomes
keywords: live-cell imaging; single-particle tracking; super-resolution imaging