title: Screening and optimization of some inorganic salts for the production of ergot alkaloids from Penicillium species using surface culture fermentation process.
abstract: The present study deals with the production of ergot alkaloids from Penicillium commune and Penicillium citrinum, using surface culture fermentation process. Impact of various inorganic salts was tested on the production of ergot alkaloids during the optimization studies of fermentation medium such as impact of various concentration levels of succinic acid, ammonium chloride, MgSO4, FeSO4, ZnSO4, pH and the effect of various incubation time periods was also determined on the production of ergot alkaloids from Penicillium commune and Penicillium citrinum. Highest yield of ergot alkaloids was obtained when Penicillium commune and Penicillium citrinum that were grown on optimum levels of ingredients such as 2 g succinic acid, 1.5 and 2 g NH4Cl, 1.5 g MgSO4, 1 g FeSO4, 1 and 1.5 g ZnSO4 after 21 days of incubation time period using pH 5 at 25(°)C incubation temperature in the fermentation medium. Ergot alkaloids were determined using Spectrophotometry and Thin Layer Chromatography (TLC) techniques.
authors.affiliation: Department of Botany, GC University, Lahore, Pakistan.
authors.forename: Memuna Ghafoor
authors.initials: MG
authors.lastname: Shahid
authors.affiliation: FBRC, PCSIR Laboratories Complex, Lahore, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Nadeem
authors.affiliation: FBRC, PCSIR Laboratories Complex, Lahore, Pakistan.
authors.forename: Shahjehan
authors.initials: S
authors.lastname: Baig
authors.affiliation: Department of Botany, GC University, Lahore, Pakistan.
authors.forename: Tanzeem Akbar
authors.initials: TA
authors.lastname: Cheema
authors.affiliation: Department of Botany, GC University, Lahore, Pakistan.
authors.forename: Saira
authors.initials: S
authors.lastname: Atta
authors.affiliation: Department of Botany, GC University, Lahore, Pakistan.
authors.forename: Gul Zareen
authors.initials: GZ
authors.lastname: Ghafoor
mesh_terms: D000643:Ammonium Chloride / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D004876:Ergot Alkaloids / Q000096:biosynthesis*; D005285:Fermentation; D005296:Ferrous Compounds / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000379:methods*; D008278:Magnesium Sulfate / Q000737:chemistry*; D010407:Penicillium / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D013053:Spectrophotometry; D019802:Succinic Acid / Q000737:chemistry; D013696:Temperature; D013997:Time Factors; D019287:Zinc Sulfate / Q000737:chemistry*
chemical_list: D004876:Ergot Alkaloids; D005296:Ferrous Compounds; D000643:Ammonium Chloride; C020748:ferrous sulfate; D008278:Magnesium Sulfate; D019287:Zinc Sulfate; D019802:Succinic Acid