title: ING4 Inhibits Proliferation and Induces Apoptosis in Human Melanoma A375 Cells via the Fas/Caspase-8 Apoptosis Pathway.
abstract: BACKGROUND
  Inhibitor of growth 4 (ING4) plays a role in regulating the cell cycle, apoptosis, cell invasion and migration, but the mechanisms involved remain to be elucidated.
  
  
  OBJECTIVE
  To explore how ING4 affects human malignant melanoma A375 cells.
  
  
  METHODS
  Recombinant lentiviral vectors (A375/pLenO-GTP-ING4) were constructed and transfected into A375 cells (experimental group). The impact of ING4 on the proliferation and apoptosis of A375 cells was investigated in in vitro and in vivo experiments in mice using the MTT assay and flow cytometry.
  
  
  RESULTS
  In the experimental group, optical density was lower and apoptotic cells were more frequent from days 2-5 (p = 0.000 and p < 0.01); there were smaller xenografts and more apoptotic cells in mice (all p < 0.05); moreover, increased levels of Fas, cleaved caspase-8 and caspase-3, and decreased levels of FasL and procyclic acidic repetitive protein were observed in vitro and in vivo.
  
  
  CONCLUSIONS
  ING4 might suppress proliferation and enhance apoptosis in human malignant melanoma cells by activating Fas-induced apoptosis in a caspase-8-dependent pathway.
authors.affiliation: Department of Dermatology, First Affiliated Hospital of Harbin Medical University, Harbin, PR China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Ma
authors.forename: Xue
authors.initials: X
authors.lastname: Cheng
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.forename: Jisheng
authors.initials: J
authors.lastname: Pan
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Hongxiao
authors.initials: H
authors.lastname: Chen
authors.forename: Yongchen
authors.initials: Y
authors.lastname: Wang
authors.forename: Limin
authors.initials: L
authors.lastname: Cai
mesh_terms: D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics*; D053181:Caspase 8 / Q000096:biosynthesis / Q000235:genetics*; D049109:Cell Proliferation; D004273:DNA, Neoplasm / Q000235:genetics*; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008546:Melanoma, Experimental / Q000188:drug therapy / Q000235:genetics / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis / Q000235:genetics*; D019014:fas Receptor / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D002352:Carrier Proteins; D004273:DNA, Neoplasm; C491899:Fas protein, mouse; C540680:ING4 protein, mouse; D011994:Recombinant Proteins; D025521:Tumor Suppressor Proteins; D019014:fas Receptor; D053181:Caspase 8