title: A methylation-switchable conformational probe for the sensitive and selective detection of RNA demethylase activity.
abstract: We describe a novel methylation-sensitive nucleic acid (RNA) probe which switches conformation according to its methylation status. When combined with a differential scanning fluorimetry technique, it enables highly sensitive and selective detection of demethylase activity at a single methylated-base level. The approach is highly versatile and may be adapted to a broad range of RNA demethylases.
authors.affiliation: Department of Pharmacy, National University of Singapore, 18 Science Drive 4, 117 543, Singapore. esther.woon@nus.edu.sg.
authors.forename: Tianming
authors.initials: T
authors.lastname: Yang
authors.forename: Adeline
authors.initials: A
authors.lastname: Cheong
authors.forename: Xiangrui
authors.initials: X
authors.lastname: Mai
authors.forename: Shui
authors.initials: S
authors.lastname: Zou
authors.forename: Esther C Y
authors.initials: EC
authors.lastname: Woon
mesh_terms: D000071496:AlkB Enzymes / Q000378:metabolism*; D001483:Base Sequence; D002942:Circular Dichroism; D005470:Fluorometry; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D012330:RNA, Double-Stranded / Q000737:chemistry / Q000378:metabolism; D044366:Transition Temperature
chemical_list: D012330:RNA, Double-Stranded; D012313:RNA; D000071496:AlkB Enzymes