title: Apparent cooperativity and apparent hyperbolic behavior of enzyme mixtures acting on the same substrate.
abstract: It is well known that a negative cooperative behavior displayed by a monomeric enzyme may be associated with the simultaneous presence of two enzymes acting on the same substrate. In this paper, emphasis is given to the effect exerted by a rapid equilibrium between the enzyme forms in leading to a hyperbolic behavior, thus masking the presence of multiple enzyme forms.
authors.affiliation: a Department of Biology-Biochemistry Unit , University of Pisa , Pisa , Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Cappiello
authors.affiliation: a Department of Biology-Biochemistry Unit , University of Pisa , Pisa , Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Balestri
authors.affiliation: a Department of Biology-Biochemistry Unit , University of Pisa , Pisa , Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Moschini
authors.affiliation: a Department of Biology-Biochemistry Unit , University of Pisa , Pisa , Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Del-Corso
authors.affiliation: a Department of Biology-Biochemistry Unit , University of Pisa , Pisa , Italy.
authors.forename: Umberto
authors.initials: U
authors.lastname: Mura
mesh_terms: D004798:Enzymes / Q000378:metabolism*; D007700:Kinetics; D013379:Substrate Specificity
chemical_list: D004798:Enzymes
keywords: Apparent cooperativity; enzyme kinetics; monomeric enzyme mixture