title: Determination of albendazole sulfoxide in human plasma by using liquid chromatography-tandem mass spectrometry.
abstract: A rapid, simple and sensitive method was developed and validated using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for determination of albendazole sulfoxide (ABZOX) in human plasma. The plasma samples were extracted by protein precipitation using albendazole sulfoxide-d3 as internal standard (IS). The chromatographic separation was performed on Waters Xbridge C18Column (100×4.6mm, 3.5μm) with a mobile phase consisting of ammonia solution, water and methanol at a flow rate of 0.70mL/min. ABZOX was detected and identified by mass spectrometry with electrospray ionization (ESI) in positive ion and multiple-reaction monitoring (MRM) mode. The method was linear in the range of 3-1500ng/mL for ABZOX. This method was successfully applied to the bioequivalence study in human plasma samples.
authors.affiliation: Novagenix Bioanalytical R&D Centre, 06970 Akyurt, Ankara, Turkey. Electronic address: nsaraner@novagenix.com.
authors.forename: Nihal
authors.initials: N
authors.lastname: Saraner
authors.affiliation: Novagenix Bioanalytical R&D Centre, 06970 Akyurt, Ankara, Turkey.
authors.forename: Güler Yağmur
authors.initials: GY
authors.lastname: Özkan
authors.affiliation: Novagenix Bioanalytical R&D Centre, 06970 Akyurt, Ankara, Turkey.
authors.forename: Berrak
authors.initials: B
authors.lastname: Güney
authors.affiliation: Novagenix Bioanalytical R&D Centre, 06970 Akyurt, Ankara, Turkey.
authors.forename: Erkin
authors.initials: E
authors.lastname: Alkan
authors.affiliation: Novagenix Bioanalytical R&D Centre, 06970 Akyurt, Ankara, Turkey.
authors.forename: Nihan
authors.initials: N
authors.lastname: Burul-Bozkurt
authors.affiliation: Novagenix Bioanalytical R&D Centre, 06970 Akyurt, Ankara, Turkey.
authors.forename: Onursal
authors.initials: O
authors.lastname: Sağlam
authors.affiliation: Novagenix Bioanalytical R&D Centre, 06970 Akyurt, Ankara, Turkey.
authors.forename: Ezgi
authors.initials: E
authors.lastname: Fikirdeşici
authors.affiliation: Novagenix Bioanalytical R&D Centre, 06970 Akyurt, Ankara, Turkey.
authors.forename: Mevlüt
authors.initials: M
authors.lastname: Yıldırım
mesh_terms: D015766:Albendazole / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D016014:Linear Models; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D015766:Albendazole; C027186:albendazole sulfoxide
keywords: Albendazole; Albendazole sulfoxide; Albendazole sulfoxide-d3; Human plasma; LC–MS/MS