title: Microarray-Based Phospho-Proteomic Profiling of Complex Biological Systems.
abstract: Protein microarray technology has been successfully used for identifying substrates of purified activated kinases. We used protein microarrays to globally interrogate the effects of PTEN and Akt activity on the phospho-kinome of in vitro and in vivo glioma models and validated results in clinical pathological specimens. Whole cell lysates extracted from tumor samples can be applied to human kinome chip microarrays to profile the global kinase phosphorylation patterns in a high-throughput manner and identify novel substrates inherent to the tumor cell and the interactions with tumor microenvironment. Our findings identify a novel microarray-based method for assessing intracellular signaling events applicable to human oncogenesis and other pathophysiologic states.
authors.affiliation: Department of Neurosurgery, The Johns Hopkins University School of Medicine, Baltimore, MD, USA; Hugo W. Moser Research Institute at Kennedy Krieger, Baltimore, MD, USA. Electronic address: rory@jhmi.edu.
authors.forename: C Rory
authors.initials: CR
authors.lastname: Goodwin
authors.affiliation: Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Crystal L
authors.initials: CL
authors.lastname: Woodard
authors.affiliation: Department of Neurology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA; Hugo W. Moser Research Institute at Kennedy Krieger, Baltimore, MD, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Ophthalmology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Pan
authors.affiliation: Department of Neurosurgery, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Olivi
authors.affiliation: Hugo W. Moser Research Institute at Kennedy Krieger, Baltimore, MD, USA.
authors.forename: Shuli
authors.initials: S
authors.lastname: Xia
authors.affiliation: Department of Neurosurgery, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Chetan
authors.initials: C
authors.lastname: Bettegowda
authors.affiliation: Department of Neurosurgery, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Sciubba
authors.affiliation: Hugo W. Moser Research Institute at Kennedy Krieger, Baltimore, MD, USA.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Pevsner
authors.affiliation: Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Heng
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Neurology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA; Hugo W. Moser Research Institute at Kennedy Krieger, Baltimore, MD, USA.
authors.forename: John
authors.initials: J
authors.lastname: Laterra