title: UHPLC-MS-based metabolomics analysis on mice bearing neoplasm (H22) for hispidulin.
abstract: Although some physiological and pathological function parameters of hepatitis and liver cancer have been investigated in relation to hispidulin (5,7,4'-trihydroxy-6-methoxyflavone), the changes of small metabolites in biofluids have been reported rarely. Recent research has shown that metabolic profiling with ultra-high-performance liquid chromatography coupled to quadrupole time of flight mass spectrometry (UHPLC-QTOF/MS) coupled with multivariate statistical analysis provides a good understanding of hispidulin effects on mice vaccinated intraperitoneally with H22 tumor cells. Twenty-five potential biomarkers, up- or down-regulated (P<0.05 or 0.01), were identified, and 17 metabolic pathways were constructed. These potential biomarkers underpin the metabolic pathways, which are disturbed in the mice bearing neoplasm (H22). These pathways include pantothenate and CoA biosynthesis; glycine, serine and threonine metabolism; nicotinate and nicotinamide metabolism; steroid hormone biosynthesis; pyrimidine metabolism; and glyoxylate and dicarboxylate metabolism. Furthermore, 4-phosphopantothenoylcysteine, glycine, niacinamide, cortisol, uracil and 5-thymidylic acid are potential biomarkers that may explain the link between hispidulin and the metabolism of mice bearing neoplasm (H22). Most of the potential biomarkers related to the function of TCA (tricarboxylic acid cycle). The rise of potential biomarkers in the drug groups promoted the up-regulation of TCA cycle compared with the model group.
authors.affiliation: Pharmaceutical Institute, Nanjing University of Chinese Medicine, Nanjing 210023, PR China. Electronic address: 18252066370@163.com.
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Li
authors.affiliation: Pharmaceutical Institute, Nanjing University of Chinese Medicine, Nanjing 210023, PR China. Electronic address: lixiang_8182@163.com.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.affiliation: Pharmaceutical Institute, Nanjing University of Chinese Medicine, Nanjing 210023, PR China. Electronic address: yunjiemiao@163.com.
authors.forename: Yunjie
authors.initials: Y
authors.lastname: Miao
authors.affiliation: Pharmaceutical Institute, Nanjing University of Chinese Medicine, Nanjing 210023, PR China. Electronic address: Thomastiger@163.com.
authors.forename: Chenxiao
authors.initials: C
authors.lastname: Shan
authors.affiliation: Pharmaceutical Institute, Nanjing University of Chinese Medicine, Nanjing 210023, PR China. Electronic address: yuanfei201092@126.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Yuan
authors.affiliation: Pharmaceutical Institute, Nanjing University of Chinese Medicine, Nanjing 210023, PR China. Electronic address: mcy199202@sina.com.
authors.forename: Chengyao
authors.initials: C
authors.lastname: Ma
authors.affiliation: Pharmaceutical Institute, Nanjing University of Chinese Medicine, Nanjing 210023, PR China. Electronic address: wangqiwen0429@163.com.
authors.forename: Qiwen
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Pharmaceutical Institute, Nanjing University of Chinese Medicine, Nanjing 210023, PR China. Electronic address: chenjw695@126.com.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Chen
authors.affiliation: Pharmaceutical Institute, Nanjing University of Chinese Medicine, Nanjing 210023, PR China. Electronic address: achenyongmail@163.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D047309:Flavones / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D047309:Flavones; C055957:hispidulin
keywords: Biomarker; Hepatocellular carcinoma; Hispidulin; Metabolomics