title: Large-scale purification of active liquid-cultured Caenorhabditis elegans using a modified Baermann apparatus.
abstract: A method for purifying active liquid-cultured Caenorhabditis elegans using a modified Baermann apparatus is described. This method consists of two parts: 1) large-scale cultivation of C. elegans in liquid medium and 2) preparation of active nematodes without contamination using the Baermann apparatus.
authors.affiliation: Department of Molecular and Cellular Parasitology, Graduate School of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan; Department of Parasitology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan. Electronic address: stakamiy@juntendo.ac.jp.
authors.forename: Shinzaburo
authors.initials: S
authors.lastname: Takamiya
authors.affiliation: Department of Molecular and Cellular Parasitology, Graduate School of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Mita
keywords: Baermann apparatus; Caenorhabditis elegans; Liquid culture