title: Raman spectroscopy as a tool for label-free lymphocyte cell line discrimination.
abstract: Unactivated lymphocytes are morphologically identical and biochemically relatively similar, making them difficult to distinguish from one another with conventional light microscopy. Here, we use Raman spectroscopy to provide biochemical information on the composition of different lymphocyte cell lines. As could be expected, the biochemical differences measured with Raman spectroscopy between lymphocyte cell lines are small, but in combination with partial least squares discriminant analysis it is possible not only to distinguish between T- and B-cells, but also between individual T-cell and B-cell lines.
authors.affiliation: Biophotonics Laboratory, Immunology Frontier Research Center, Osaka University, 3-1 Yamada-oka, Suita, Osaka, 565-0871, Japan. ahobro@ifrec.osaka-u.ac.jp nsmith@ap.eng.osaka-u.ac.jp.
authors.forename: Alison J
authors.initials: AJ
authors.lastname: Hobro
authors.forename: Yutaro
authors.initials: Y
authors.lastname: Kumagai
authors.forename: Shizuo
authors.initials: S
authors.lastname: Akira
authors.forename: Nicholas I
authors.initials: NI
authors.lastname: Smith
mesh_terms: D002460:Cell Line; D016002:Discriminant Analysis; D006801:Humans; D016018:Least-Squares Analysis; D008214:Lymphocytes / Q000166:cytology*; D061848:Optical Imaging; D013059:Spectrum Analysis, Raman