title: Quantification of Dopamine in Brain Microdialysates with High-Performance Liquid Chromatography-Tandem Mass Spectrometry.
abstract: The dopamine level in the brain and the mesolimbick dopaminergic system are responsible for nicotine addiction. In the present study, extracellular dopamine in nucleus accumbens was collected by a brain microdialysis technique. Also a sensitive high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method with a gradient elution procedure was developed for a precise determination of dopamine in brain dialysate. The retention time of dopamine was about 11.32 min. The linear range was 20 - 1000 pg/mL. The limit of detection (LOD) and the limit of qualification (LOQ) were 5 and 20 pg/mL, respectively. The recovery ranged from 98.2 to 109.0%, and both the intra-RSD and inter-RSD were below <9%, respectively. The probe recovery for dopamine in this brain microdialysis experiment was about 25%. Finally, the dopamine concentrations in the rat brain microdialysates were determined, and the pharmacokinetics of extracellular dopamine in the brain nucleus accumbens after an intravenous injection of nicotine was successfully evaluated.
authors.affiliation: Technical Center of Shanghai Tobacco Group Co. Ltd.
authors.forename: Dawei
authors.initials: D
authors.lastname: Qi
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Wanhong
authors.initials: W
authors.lastname: Zhou
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zhao
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Sha
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Pang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004298:Dopamine / Q000032:analysis*; D008297:Male; D017551:Microdialysis; D009538:Nicotine / Q000008:administration & dosage / Q000494:pharmacology; D009714:Nucleus Accumbens / Q000737:chemistry* / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009538:Nicotine; D004298:Dopamine