title: Deletion of JJJ1 improves acetic acid tolerance and bioethanol fermentation performance of Saccharomyces cerevisiae strains.
abstract: OBJECTIVE
  To improve tolerance to acetic acid that is present in lignocellulosic hydrolysates and affects bioethanol production by Saccharomyces cerevisiae.
  
  
  RESULTS
  Saccharomyces cerevisiae strains with improved tolerance to acetic acid were obtained through deletion of the JJJ1 gene. The lag phase of the JJJ1 deletion mutant BYΔJJJ1 was ~16 h shorter than that of the parent strain, BY4741, when the fermentation medium contained 4.5 g acetic acid/l. Additionally, the specific ethanol production rate of BYΔJJJ1 was increased (0.057 g/g h) compared to that of the parent strain (0.051 g/g h). Comparative transcription and physiological analyses revealed higher long chain fatty acid, trehalose, and catalase contents might be critical factors responsible for the acetic acid resistance of JJJ1 knockout strains.
  
  
  CONCLUSIONS
  JJJ1 deletion improves acetic acid tolerance and ethanol fermentation performance of S. cerevisiae.
authors.affiliation: College of Life Science, Zhejiang University, Hangzhou, 310058, Zhejiang Province, China.
authors.forename: Xuechang
authors.initials: X
authors.lastname: Wu
authors.affiliation: College of Life Science, Zhejiang University, Hangzhou, 310058, Zhejiang Province, China.
authors.forename: Lijie
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Life Science, Zhejiang University, Hangzhou, 310058, Zhejiang Province, China.
authors.forename: Xinna
authors.initials: X
authors.lastname: Jin
authors.affiliation: College of Life Science, Zhejiang University, Hangzhou, 310058, Zhejiang Province, China.
authors.forename: Yahong
authors.initials: Y
authors.lastname: Fang
authors.affiliation: College of Life Science, Zhejiang University, Hangzhou, 310058, Zhejiang Province, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Ocean College, Zhejiang University, Hangzhou, 310058, Zhejiang Province, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Qi
authors.affiliation: Ocean College, Zhejiang University, Hangzhou, 310058, Zhejiang Province, China. zhengdaoqiong@zju.edu.cn.
authors.forename: Daoqiong
authors.initials: D
authors.lastname: Zheng
mesh_terms: D019342:Acetic Acid / Q000494:pharmacology*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D000431:Ethanol; D019342:Acetic Acid
keywords: Acetic acid; Bioethanol; JJJ1; Saccharomyces cerevisiae; Tolerance