title: Decreased Expression of BRCA2 Accelerates Sporadic Breast Cancer Progression.
abstract: Human tumor suppressor BRCA2 has been known to function in the repair of DNA double strand break. Germ line mutation in BRCA genes predisposes individuals to familial breast and ovarian cancer. The aim of present study was to characterize the implication of BRCA2 expression in cases of non - hereditary (sporadic) breast cancer. Female breast cancer patients aged between 20 and 75 years who underwent surgery were randomly selected. Patients with Stage IV disease at time of primary diagnosis or previous history of any other malignancy other than breast carcinoma or undergoing neoadjuvant chemotherapy were excluded from the study. Total 48 patients full filled these criteria. Patients were treated with either modified radical mastectomy or breast conservation therapy. Concentration of BRCA2 was measured by Sandwiched method of ELISA. Immunohistochemistry was used to determine the hormonal receptor status. Stage of the disease was not found to have any significant correlation on the BRCA2 expression. In patients with higher grade of tumors the level of BRCA2 expression was found to be low. Decreased expression of BRCA2 was found to be triple negative, had aggressive features and associated with higher chances of axillary metastasis. Genetic instability caused by decreased expression of BRCA2 could trigger mutations in sporadic breast cancer cases and mutations in turn leads to uncontrolled proliferation and invasive growth.
authors.affiliation: Departement of General Surgery, IPGME&R/SSKMH, 244, A.J.C Bose Road, 700020 Kolkata, India.
authors.forename: Soumi
authors.initials: S
authors.lastname: Saha
authors.affiliation: Departement of General Surgery, IPGME&R/SSKMH, 244, A.J.C Bose Road, 700020 Kolkata, India.
authors.forename: Pranab
authors.initials: P
authors.lastname: Mandal
authors.affiliation: Departement of General Surgery, IPGME&R/SSKMH, 244, A.J.C Bose Road, 700020 Kolkata, India.
authors.forename: Suvro
authors.initials: S
authors.lastname: Ganguly
authors.affiliation: Departement of General Surgery, IPGME&R/SSKMH, 244, A.J.C Bose Road, 700020 Kolkata, India.
authors.forename: Debarshi
authors.initials: D
authors.lastname: Jana
authors.affiliation: Departement of General Surgery, IPGME&R/SSKMH, 244, A.J.C Bose Road, 700020 Kolkata, India.
authors.forename: Asif
authors.initials: A
authors.lastname: Ayaz
authors.affiliation: Departement of General Surgery, IPGME&R/SSKMH, 244, A.J.C Bose Road, 700020 Kolkata, India.
authors.forename: Abhirup
authors.initials: A
authors.lastname: Banerjee
authors.affiliation: Departement of General Surgery, IPGME&R/SSKMH, 244, A.J.C Bose Road, 700020 Kolkata, India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Chouhan
authors.affiliation: Departement of General Surgery, IPGME&R/SSKMH, 244, A.J.C Bose Road, 700020 Kolkata, India.
authors.forename: Diptendra Kumar
authors.initials: DK
authors.lastname: Sarkar
keywords: BRCA2 decreased expression; Double Strand Break (DSB); Homology-directed Recombination (HR); Triple Negative Breast Cancer (TNBC)