title: Sarcodon aspratus Extract Ameliorates Dextran Sulfate Sodium-Induced Colitis in Mouse Colon and Mesenteric Lymph Nodes.
abstract: Mushrooms have been previously investigated for their immune-modulating and anti-inflammatory properties. We examined whether the anti-inflammatory properties of Sarcodon aspratus ethanol extract (SAE) could elicit protective effects against dextran sulfate sodium (DSS)-induced colitis in vivo. Male C57/BL6 mice were randomly assigned to 1 of 4 treatment groups: control (CON; n = 8), DSS-treated (DSS; n = 9), DSS+SAE at 50 mg/kg BW (SAE50; n = 8), and DSS+SAE at 200 mg/kg BW groups (SAE200; n = 9). DSS treatment induced significant weight loss, which was significantly recovered by SAE200. Although SAE did not affect DSS-mediated reductions in colon length, it improved diarrhea and rectal bleeding induced by DSS. SAE at 200 mg/kg BW significantly attenuated IL-6 and enhanced IL-10 expression in mesenteric lymph nodes (MLN), and significantly reduced IL-6 levels in splenocytes. SAE200 also significantly attenuated DSS-induced increase in IL-6 and IL-1β, and reductions in IL-10 in colon tissue. High levels of SAE were also observed to significantly decrease inflammatory COX-2 expression that was upregulated by DSS in mice colon. These findings may have relevance for novel therapeutic strategies to mitigate inflammatory bowel disease-relevant inflammatory responses, via the direct and indirect anti-inflammatory activity of SAE. We also found that SAE harbors significant quantities of total fiber and β-glucan, suggesting a possible role for these components in protection against DSS-mediated colitis.
authors.affiliation: Div. of Nutrition and Metabolism Research, Korea Food Research Inst, Seongnam, Republic of Korea.
authors.forename: Min-Yu
authors.initials: MY
authors.lastname: Chung
authors.affiliation: Dept. of Food Biotechnology, Korea Univ. of Science & Technology, Daejeon, Republic of Korea.
authors.forename: Jin-Taek
authors.initials: JT
authors.lastname: Hwang
authors.affiliation: Div. of Nutrition and Metabolism Research, Korea Food Research Inst, Seongnam, Republic of Korea.
authors.forename: Jin Hee
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Div. of Nutrition and Metabolism Research, Korea Food Research Inst, Seongnam, Republic of Korea.
authors.forename: Dong-Hwa
authors.initials: DH
authors.lastname: Shon
authors.affiliation: Div. of Nutrition and Metabolism Research, Korea Food Research Inst, Seongnam, Republic of Korea.
authors.forename: Hyun-Ku
authors.initials: HK
authors.lastname: Kim
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001487:Basidiomycota / Q000737:chemistry* / Q000378:metabolism; D001688:Biological Products / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D003106:Colon / Q000187:drug effects* / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism*; D016264:Dextran Sulfate; D004043:Dietary Fiber / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism; D015212:Inflammatory Bowel Diseases / Q000188:drug therapy / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008198:Lymph Nodes / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008643:Mesentery; D051379:Mice; D008810:Mice, Inbred C57BL; D011897:Random Allocation; D047071:beta-Glucans / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D000893:Anti-Inflammatory Agents; D001688:Biological Products; D016207:Cytokines; D004043:Dietary Fiber; D053583:Interleukin-1beta; D015850:Interleukin-6; D047071:beta-Glucans; D016753:Interleukin-10; D016264:Dextran Sulfate; D051546:Cyclooxygenase 2
keywords: Sarcodon aspratus; T cells; beta-glucan; dextran sulfate sodium; inflammation