title: Eighteen new oleaginous yeast species.
abstract: Of 1600 known species of yeasts, about 70 are known to be oleaginous, defined as being able to accumulate over 20 % intracellular lipids. These yeasts have value for fundamental and applied research. A survey of yeasts from the Phaff Yeast Culture Collection, University of California Davis was performed to identify additional oleaginous species within the Basidiomycota phylum. Fifty-nine strains belonging to 34 species were grown in lipid inducing media, and total cell mass, lipid yield and triacylglycerol profiles were determined. Thirty-two species accumulated at least 20 % lipid and 25 species accumulated over 40 % lipid by dry weight. Eighteen of these species were not previously reported to be oleaginous. Triacylglycerol profiles were suitable for biodiesel production. These results greatly expand the number of known oleaginous yeast species, and reveal the wealth of natural diversity of triacylglycerol profiles within wild-type oleaginous Basidiomycetes.
authors.affiliation: Phaff Yeast Culture Collection, Department of Food Science and Technology, University of California, One Shields Ave, Davis, CA, 95616-8598, USA.
authors.forename: Luis A
authors.initials: LA
authors.lastname: Garay
authors.affiliation: Phaff Yeast Culture Collection, Department of Food Science and Technology, University of California, One Shields Ave, Davis, CA, 95616-8598, USA.|Bioentrepreneurship Department, Indonesia International Institute for Life Sciences, Jalan Pulo Mas Barat Kav. 88, East Jakarta, DKI Jakarta, 13210, Indonesia.
authors.forename: Irnayuli R
authors.initials: IR
authors.lastname: Sitepu
authors.affiliation: Metabolomics, UC Davis Genome Center, University of California Davis, 451 Health Sciences Drive, Davis, CA, 95616, USA.
authors.forename: Tomas
authors.initials: T
authors.lastname: Cajka
authors.affiliation: Phaff Yeast Culture Collection, Department of Food Science and Technology, University of California, One Shields Ave, Davis, CA, 95616-8598, USA.
authors.forename: Idelia
authors.initials: I
authors.lastname: Chandra
authors.affiliation: Phaff Yeast Culture Collection, Department of Food Science and Technology, University of California, One Shields Ave, Davis, CA, 95616-8598, USA.
authors.forename: Sandy
authors.initials: S
authors.lastname: Shi
authors.affiliation: Phaff Yeast Culture Collection, Department of Food Science and Technology, University of California, One Shields Ave, Davis, CA, 95616-8598, USA.
authors.forename: Ting
authors.initials: T
authors.lastname: Lin
authors.affiliation: Department of Food Science and Technology, University of California, One Shields Ave, Davis, CA, 95616, USA.
authors.forename: J Bruce
authors.initials: JB
authors.lastname: German
authors.affiliation: Metabolomics, UC Davis Genome Center, University of California Davis, 451 Health Sciences Drive, Davis, CA, 95616, USA.|Biochemistry Department, Faculty of Science, King Abdulaziz University, PO Box 80203, Jeddah, 21589, Saudi Arabia.
authors.forename: Oliver
authors.initials: O
authors.lastname: Fiehn
authors.affiliation: Phaff Yeast Culture Collection, Department of Food Science and Technology, University of California, One Shields Ave, Davis, CA, 95616-8598, USA. klbmills@ucdavis.edu.
authors.forename: Kyria L
authors.initials: KL
authors.lastname: Boundy-Mills
mesh_terms: D001487:Basidiomycota / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D056804:Biofuels; D003470:Culture Media / Q000737:chemistry; D008055:Lipids / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000145:classification / Q000378:metabolism; D014280:Triglycerides / Q000096:biosynthesis; D015003:Yeasts / Q000145:classification* / Q000378:metabolism*
chemical_list: D056804:Biofuels; D003470:Culture Media; D008055:Lipids; D014280:Triglycerides
keywords: Basidiomycete; Biodiesel; Intracellular lipid; Oleaginous yeast; Triacylglycerol