title: Expressional and Bioinformatic Analysis of Bovine Filia/Ecat1/Khdc3l Gene: A Comparison with Ovine Species.
abstract: Maternal effect genes have highly impressive effects on pre-implantation development. Filia/Ecat1/Khdc3l is a maternal effect gene found in mouse oocytes and embryos, loss of which causes a 50% decrease in fertility. In the present study, we investigated Filia mRNA expression in bovine oviduct, 30- to 40-day fetus, liver, heart, lung, and oocytes (as a positive control), by RT-PCR and detected it only in oocytes. A 443 bp fragment was amplified only in oocytes and was sequenced as a part of bovine predicted Filia mRNA. We analyzed bovine and ovine Filia N-terminal peptide sequence in PHYRE2, and a KH domain was predicted. Protein alignment using ClustalW indicated a highly identical N-terminal extention between the 2 species. Immunohistochemical analysis using anti-bovine Filia antibody showed the expression of Filia protein in the zone surrounding the nuclear membrane, and in the subcortex of ovine oocytes of primary and antral follicles. However, in the bovine, Filia has been found through the oocyte cytoplasm of antral follicles, and here it is further confirmed in the primary follicles. Our data suggests a difference in Filia expression pattern between cow and sheep, although the sequence is highly conserved.
authors.affiliation: a Department of Animal Science, College of Agriculture , Isfahan University of Technology , Isfahan , Iran.
authors.forename: Azadeh
authors.initials: A
authors.lastname: Zahmatkesh
authors.affiliation: a Department of Animal Science, College of Agriculture , Isfahan University of Technology , Isfahan , Iran.
authors.forename: Saeid
authors.initials: S
authors.lastname: Ansari Mahyari
authors.affiliation: b Department of Animal Biotechnology , Institute of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology , Tehran , Iran.
authors.forename: Morteza
authors.initials: M
authors.lastname: Daliri Joupari
authors.affiliation: a Department of Animal Science, College of Agriculture , Isfahan University of Technology , Isfahan , Iran.
authors.forename: Hamidreza
authors.initials: H
authors.lastname: Rahmani
authors.affiliation: c Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR , Tehran , Iran.
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Shirazi
authors.affiliation: d Department of Animal Science, Faculty of Agriculture , Shahid Bahonar University of Kerman , Kerman , Iran.
authors.forename: Mahmood
authors.initials: M
authors.lastname: Amiri Roudbar
authors.affiliation: b Department of Animal Biotechnology , Institute of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology , Tehran , Iran.
authors.forename: Saeid
authors.initials: S
authors.lastname: Ansari Majd
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D010053:Ovary / Q000737:chemistry / Q000378:metabolism*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D012756:Sheep / Q000235:genetics* / Q000378:metabolism
chemical_list: D011506:Proteins
keywords: Bovine tissues; KH domain; ovine ovary; pre-implantation development; protein localization