title: Spatial and temporal expression patterns of Osbpl2a and Osbpl2b during zebrafish embryonic development.
abstract: OBJECTIVE
  The mutated OSBPL2 (OMIM: 606731), encoding oxysterol binding protein-like protein 2, was recently identified as a novel causative gene for autosomal dominant nonsyndromic hearing loss (ADNSHL). We reported the expression patterns of Osbpl2 in zebrafish, in order to further understand the role of OSBPL2 in hearing formation and development.
  
  
  METHODS
  Zebrafish was used as an animal model, and the expression of Osbpl2 was investigated by whole mount in situ hybridization.
  
  
  RESULTS
  Bioinformatics analysis indicates that zebrafish has two homologues of Osbpl2 gene (Osbpl2a and Osbpl2b) and Osbpl2b is the orthologous gene of human OSBPL2. No expression of Osbpl2a and Osbpl2b mRNA is detected at 75% epiboly. The zygotical expression of the two genes has not been started at 11-somite stage. At 24h post-fertilization (hpf), both Osbpl2a and Osbpl2b are found at ventricle zone of brain, however, the expression level of Osbpl2a is higher than that of Osbpl2b. When embryos are 48hpf, the expression level of Osbpl2a and Osbpl2b becomes higher at the ventricle zone. At 72hpf, Osbpl2b is only found at liver primordium, while Osbpl2a is not detected anywhere obviously. At 96hpf, Osbpl2b is found at pharyngeal arches, liver, digestive tract and otic vesicle, while Osbpl2a remains undetected.
  
  
  CONCLUSIONS
  Osbpl2b was demonstrated to be the orthologous gene of human OSBPL2, which has strong maternal expression, while Osbpl2a was detected without obvious maternal expression. This work would contribute to the further study of the molecular mechanism and function of OSBPL2 implicated with ADNSHL.
authors.affiliation: Department of Biotechnology, School of Basic Medicine, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Chunyu
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Biotechnology, School of Basic Medicine, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yao
authors.affiliation: Department of Biotechnology, School of Basic Medicine, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Qinjun
authors.initials: Q
authors.lastname: Wei
authors.affiliation: Department of Otolaryngology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China. Electronic address: xing-gq@163.com.
authors.forename: Guangqian
authors.initials: G
authors.lastname: Xing
authors.affiliation: Department of Biotechnology, School of Basic Medicine, Nanjing Medical University, Nanjing 210029, China. Electronic address: caoxin@njmu.edu.cn.
authors.forename: Xin
authors.initials: X
authors.lastname: Cao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005819:Genetic Markers; D006319:Hearing Loss, Sensorineural / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D011987:Receptors, Steroid / Q000235:genetics* / Q000378:metabolism; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism; D000098825:Oxysterol Binding Proteins
chemical_list: D005819:Genetic Markers; C461064:OSBPL2 protein, human; C000612189:Osbpl2a protein, zebrafish; C000612190:Osbpl2b protein, zebrafish; D011987:Receptors, Steroid; D029961:Zebrafish Proteins; D000098825:Oxysterol Binding Proteins
keywords: Early development; Osbpl2a; Osbpl2b; Spatial and temporal expression; Zebrafish