title: Genotoxicity of a thiosulfonate compound derived from Allium sp. intended to be used in active food packaging: In vivo comet assay and micronucleus test.
abstract: Components of Allium species have antimicrobial and antioxidant properties. A commercial Allium sp. extract (Proallium AP(®)), of which the main constituent is propyl thiosulphinate oxide (PTSO), is being used in the development of active food packaging. In previous in vitro genotoxicity studies, PTSO, in the presence of metabolic activation, increased the appearance of micronuclei (MN). We assessed the genotoxicity PTSO in rats following oral administration (doses: 5.5, 17.4, and 55mg/kg). The comet assay in liver and stomach (OECD 489) and the MN assay in bone marrow (OECD 474) were carried out. After necropsy, histopathological examinations of the liver and the stomach were performed. The results revealed no in vivo genotoxicity and the histopathological analysis showed only slight modifications, such as increased glycogen storage in the liver and a degenerative process in stomach, with vacuolization of cell membranes, only at the highest dose. Therefore, the present work confirms that this compound is not genotoxic and could be considered as a natural alternative to synthetic preservatives used in the food packaging industry.
authors.affiliation: Area of Toxicology, Faculty of Pharmacy, Universidad de Sevilla, Profesor García González n°2, 41012 Seville, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Mellado-García
authors.affiliation: Area of Toxicology, Faculty of Pharmacy, Universidad de Sevilla, Profesor García González n°2, 41012 Seville, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Puerto
authors.affiliation: Area of Toxicology, Faculty of Pharmacy, Universidad de Sevilla, Profesor García González n°2, 41012 Seville, Spain.
authors.forename: Ana I
authors.initials: AI
authors.lastname: Prieto
authors.affiliation: Area of Toxicology, Faculty of Pharmacy, Universidad de Sevilla, Profesor García González n°2, 41012 Seville, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Pichardo
authors.affiliation: Area of Toxicology, Faculty of Pharmacy, Universidad de Sevilla, Profesor García González n°2, 41012 Seville, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Martín-Cameán
authors.affiliation: Department of Pharmacology, Toxicology and Legal and Forensic Medicine, Faculty of Veterinary Medicine, University of Córdoba, Campus de Rabanales, Córdoba, Spain.
authors.forename: Rosario
authors.initials: R
authors.lastname: Moyano
authors.affiliation: Department of Anatomy and Comparative Pathology and Anatomy, University of Córdoba, Campus de Rabanales, Córdoba, Spain.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Blanco
authors.affiliation: Area of Toxicology, Faculty of Pharmacy, Universidad de Sevilla, Profesor García González n°2, 41012 Seville, Spain. Electronic address: camean@us.es.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Cameán
mesh_terms: D000490:Allium / Q000737:chemistry*; D000818:Animals; D020552:Comet Assay / Q000379:methods*; D005260:Female; D018857:Food Packaging; D008401:Gas Chromatography-Mass Spectrometry; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D015162:Micronucleus Tests / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D009152:Mutagenicity Tests; D017208:Rats, Wistar; D013270:Stomach / Q000187:drug effects / Q000473:pathology; D013441:Sulfinic Acids / Q000097:blood / Q000378:metabolism / Q000633:toxicity*
chemical_list: D013441:Sulfinic Acids; C000608757:propyl thiosulfinate oxide
keywords: Allium sp.; Comet assay; Genotoxicity; Micronucleus; Propyl thiosulphinate oxide; Rat