title: [Effects of exendin-4 on extracellular matrix metabolism in human mesangial cells cultured in high glucose].
abstract: OBJECTIVE
  To explore effects of exendin-4 on the metabolism of extracellular matrix (ECM) in human mesangial cells (HMC) cultured in the presence of high glucose and explore the possible mechanism.
  
  
  METHODS
  Human mesangial cells (HMC) were treated with exendin-4 under high glucose conditions. The cell proliferation was observed using CCK8 assay, and the expressions of collagen type I, fibronectin, transforming growth factor-β1 (TGFβ1) expression and extracellular signal- regulated kinase (ERK) signaling pathway activity were assessed using Western blotting.
  
  
  RESULTS
  Exendin-4 inhibited cell proliferation and the expressions of collagen type I, fibronectin and TGFβ1 and reversed ERK phosphorylation in high glucose-induced HMC.
  
  
  CONCLUSIONS
  Exendin-4 can regulate ECM metabolism in HMC cultured in high glucose by inhibiting TGFβ1/ERK pathway, suggesting the beneficial effects of exendin-4 in preventing and treating diabetic nephropathy.
authors.affiliation: Department of Endocrinology and Metabolism, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.E-mail: xiaozhizhou0701@163.com.
authors.forename: Zhi-Zhou
authors.initials: ZZ
authors.lastname: Xiao
authors.forename: Mei-Ping
authors.initials: MP
authors.lastname: Guan
authors.forename: Zong-Ji
authors.initials: ZJ
authors.lastname: Zheng
authors.forename: Yi-Jie
authors.initials: YJ
authors.lastname: Jia
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Yao-Ming
authors.initials: YM
authors.lastname: Xue
mesh_terms: D049109:Cell Proliferation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D003928:Diabetic Nephropathies; D000077270:Exenatide; D005109:Extracellular Matrix / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D005947:Glucose / Q000737:chemistry; D006801:Humans; D020935:MAP Kinase Signaling System; D050527:Mesangial Cells / Q000187:drug effects*; D010455:Peptides / Q000494:pharmacology*; D010766:Phosphorylation; D015398:Signal Transduction; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014688:Venoms / Q000494:pharmacology*
chemical_list: D024042:Collagen Type I; D003470:Culture Media; D005353:Fibronectins; D010455:Peptides; D053773:Transforming Growth Factor beta1; D014688:Venoms; D000077270:Exenatide; D005947:Glucose