title: Effect of poly-glutamate on uptake efficiency and cytotoxicity of cell penetrating peptides.
abstract: Cell penetrating peptides (CPPs) were developed as vehicles for efficient delivery of various molecules. An ideal CPP-peptide should not display any toxicity against cancer cells as well as healthy cells and efficiently enter into the cell. Because of the cationic nature and the intrinsic vector capabilities, these peptides can cause cytotoxicity. One of the possible reasons for toxicity of CPPs is direct translocation and consequently, pore formation on the plasma membrane. In this study it was demonstrated that interaction of poly-glutamate with CPP considerably reduced their cytotoxicity in A549 cell. This strategy could be useful for efficient drug delivery mediated by CPP.
authors.affiliation: Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Samad Mussa
authors.initials: SM
authors.lastname: Farkhani
authors.affiliation: Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Shirani
authors.affiliation: Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Samaneh
authors.initials: S
authors.lastname: Mohammadi
authors.affiliation: Liver and Gastrointestinal Diseases Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Parvin
authors.initials: P
authors.lastname: Zakeri-Milani
authors.affiliation: Biotechnology Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Javid
authors.initials: J
authors.lastname: Shahbazi Mojarrad
authors.affiliation: Drug Applied Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran. valizadeh@tbzmed.ac.ir.
authors.forename: Hadi
authors.initials: H
authors.lastname: Valizadeh
mesh_terms: D000595:Amino Acid Sequence / Q000502:physiology; D002463:Cell Membrane Permeability / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D057846:Cell-Penetrating Peptides / Q000737:chemistry* / Q000493:pharmacokinetics*; D016503:Drug Delivery Systems; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry / Q000493:pharmacokinetics; D006801:Humans; D008422:Materials Testing; D008856:Microscopy, Fluorescence; D058726:Nanoconjugates / Q000737:chemistry / Q000633:toxicity; D011099:Polyglutamic Acid / Q000737:chemistry* / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D057846:Cell-Penetrating Peptides; D058726:Nanoconjugates; D011099:Polyglutamic Acid; D016650:Fluorescein-5-isothiocyanate