title: Regulatory B10 cells play a protective role in severe acute pancreatitis.
abstract: BACKGROUND
  B10 cells are specific B cell subsets with the capacity of producing IL-10 to inhibit immune responses. Several studies have demonstrated that B10 cells are correlated with some immune and inflammatory diseases, such as experimental autoimmune encephalomyelitis (EAE), collagen-induced arthritis (CA), colitis and contact hypersensitivity. However, its role in severe acute pancreatitis (SAP) has not been clearly demonstrated yet.
  
  
  OBJECTIVE
  In this study, we show that B10 cells can inhibit inflammation of severe acute pancreatitis (SAP).
  
  
  METHODS
  Blood from 17 patients with SAP and 22 age-matched healthy volunteers were collected to detect the proportion of B10 cells. CD19-/- mice were used as B10 cell-deficient mice. Amylase and lipase levels, pancreatic edema and HE staining were tested to assess the severity of SAP.
  
  
  RESULTS
  CD19-/- mice, which lack B10 cells, suffered a more severe inflammation in pancreas compared with wild-type mice after caerulein injection. The frequency of B10 cells was decreased both in SAP patients and SAP animal models. Adoptive transfer of B10 cells ameliorates inflammatory injury of pancreatitis in CD19-/- mice.
  
  
  CONCLUSIONS
  Thus, we identified B10 cells as a protective factor for SAP and provided a novel target for SAP treatment.
authors.affiliation: State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: Zhaoyan
authors.initials: Z
authors.lastname: Qiu
authors.affiliation: State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Bai
authors.affiliation: State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Hao
authors.affiliation: State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: Shiqi
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: Zhanwei
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: Zhenning
authors.initials: Z
authors.lastname: Hang
authors.affiliation: State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: Min
authors.initials: M
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, Xi'an, China. fengqx@fmmu.edu.cn.
authors.forename: Quanxin
authors.initials: Q
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, Xi'an, China. zhaoqc@fmmu.edu.cn.
authors.forename: Qingchuan
authors.initials: Q
authors.lastname: Zhao
mesh_terms: D000293:Adolescent; D019264:Adoptive Transfer; D000328:Adult; D000368:Aged; D000818:Animals; D018941:Antigens, CD19 / Q000235:genetics; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D002108:Ceruletide; D005260:Female; D006801:Humans; D008297:Male; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008875:Middle Aged; D010179:Pancreas / Q000473:pathology; D010195:Pancreatitis / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D055815:Young Adult
chemical_list: D018941:Antigens, CD19; C000604812:CD19 antigen, mouse; D002108:Ceruletide
keywords: Adoptive transfer; B10 cells; Inflammation; Severe acute pancreatitis