title: Effects of Hyriopsis cumingii Polysaccharides on Mice Immunologic Receptor, Transcription Factor, and Cytokine.
abstract: To discuss the molecular mechanism of immunoenhancing activities of Hyriopsis cumingii polysaccharides (HCPS), effects of HCPS on mice immunologic receptors (toll-like receptors-4 [TLR-4] and mannose receptor-1 [MR-1]), transcription factor (nuclear factor kappa-B [NF-κB]), and cytokines (interleukin-6 [IL-6] and tumor necrosis factor-α [TNF-α]) were evaluated by cell model in vitro and cyclophosphamide-induced immunosuppression animal model in vivo. Results showed that HCPS could promote the mRNA synthesis of TLR-4, MR-1, IL-6, and TNF-α in spleen, and the gene expression of TLR-4, MR-1, NF-κB, IL-6, and TNF-α in spleen and serum in a dose-dependent manner. Crude HCPS and its purified fractions (HCPS-1, HCPS-2, and HCPS-3) could strengthen peritoneal macrophage expressing MR-1 and NF-κB in a dose-dependent manner. In addition, HCPS-3 showed stronger promotions on MR-1 and NF-κB than crude HCPS, HCPS-1, and HCPS-2. It suggested that HCPS-stimulated immunostrengthening was mediated, at least in part, by TLR-4/NF-κB/IL-6 and TLR-4/NF-κB/ TNF-α signaling pathways. MR-1, IL-6, and TNF-α might be 3 of the immune regulators mediating immunity and homeostasis when HCPS performed immunoenhancing activities.
authors.affiliation: College of Biological and Pharmaceutical Engineering, West Anhui Univ, Lu'an, 237012, PR China.
authors.forename: Deliang
authors.initials: D
authors.lastname: Qiao
authors.affiliation: College of Biological and Pharmaceutical Engineering, West Anhui Univ, Lu'an, 237012, PR China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: He
authors.affiliation: College of Biological and Pharmaceutical Engineering, West Anhui Univ, Lu'an, 237012, PR China.
authors.forename: Chuanbao
authors.initials: C
authors.lastname: Wei
authors.affiliation: College of Biological and Pharmaceutical Engineering, West Anhui Univ, Lu'an, 237012, PR China.
authors.forename: Lunbin
authors.initials: L
authors.lastname: Xia
authors.affiliation: College of Biological and Pharmaceutical Engineering, West Anhui Univ, Lu'an, 237012, PR China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Bao
mesh_terms: D000818:Animals; D001688:Biological Products / Q000494:pharmacology; D049872:Bivalvia / Q000737:chemistry*; D016207:Cytokines / Q000378:metabolism; D005260:Female; D056747:Immunomodulation; D015850:Interleukin-6 / Q000378:metabolism*; D037181:Lectins, C-Type / Q000378:metabolism*; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000378:metabolism*; D008815:Mice, Inbred Strains; D016328:NF-kappa B / Q000378:metabolism*; D011134:Polysaccharides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism*; D015398:Signal Transduction; D051197:Toll-Like Receptor 4 / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D001688:Biological Products; D016207:Cytokines; D015850:Interleukin-6; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D016328:NF-kappa B; D011134:Polysaccharides; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D014157:Transcription Factors; D014409:Tumor Necrosis Factor-alpha; C577686:interleukin-6, mouse
keywords: Hyriopsis cumingii; cytokine; immunologic receptor; polysaccharides; transcription factor