title: Dietary Phenolic Acids of Macrotyloma uniflorum (Horse Gram) Protect the Rat Heart Against Isoproterenol-Induced Myocardial Infarction.
abstract: The present study investigates the cardioprotective activity of the Macrotyloma uniflorum seed extract (MUSE) and its phenolic acids (p-coumaric acid and ferulic acid) in isoproterenol (ISO)-induced myocardial infarction in rats. The previously mentioned phenolic acids were isolated and quantified from MUSE by HPLC. Pretreatment of gemfibrozil (reference standard), MUSE (250 and 500 mg/kg) and the phenolic acids for 30 days to rats treated with ISO (85 mg/kg) on the last 2 days resulted in a significant attenuation of the ISO-elevated levels of serum marker enzymes (aspartate aminotransferase, lactate dehydrogenase and creatine phosphokinase MB), total cholesterol, triglycerides, uric acid, C-reactive protein and malondialdehyde and a restoration of the levels of the ISO-depleted marker enzymes, reduced glutathione and the antioxidant enzymes-superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase in heart. Restoration of the ISO-altered electrocardiogram pattern and haemodynamic parameters (left ventricular end diastolic pressure, heart rate, systolic, diastolic and mean arterial pressure) was also brought about by treatment with MUSE and the phenolic acids. It may be concluded that MUSE treatment to ISO-challenged rats exhibits a significant cardioprotective effect probably because of the potent antioxidant activity of its phenolic acids that salvage the myocardium from the deleterious effects of ISO. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Pharmacology and Toxicology, Prin. K. M. Kundnani College of Pharmacy, Jote Joy Building, Rambhau Salgaonkar Marg, Cuffe Parade, Colaba, Mumbai, 400005, India.
authors.forename: Vandana
authors.initials: V
authors.lastname: Panda
authors.affiliation: Department of Pharmacology and Toxicology, Prin. K. M. Kundnani College of Pharmacy, Jote Joy Building, Rambhau Salgaonkar Marg, Cuffe Parade, Colaba, Mumbai, 400005, India.
authors.forename: Ankit
authors.initials: A
authors.lastname: Laddha
authors.affiliation: Department of Pharmacology, SPP School of Pharmacy and Technology Management, SVKM's NMIMS, Vile Parle (W), Mumbai, 400056, India.
authors.forename: Mukesh
authors.initials: M
authors.lastname: Nandave
authors.affiliation: Department of Pathology, Dr. D.Y. Patil Medical College, Navi, Mumbai, India.
authors.forename: Sudhamani
authors.initials: S
authors.lastname: Srinath
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D007887:Fabaceae / Q000737:chemistry*; D006321:Heart / Q000187:drug effects; D062385:Hydroxybenzoates / Q000008:administration & dosage*; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D009203:Myocardial Infarction / Q000517:prevention & control*; D010936:Plant Extracts / Q000494:pharmacology; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D062385:Hydroxybenzoates; D010936:Plant Extracts; D020011:Protective Agents; C017616:phenolic acid; D007545:Isoproterenol
keywords: Macrotyloma uniflorum seed; cardioprotective activity; ferulic acid; isoproterenol; p-coumaric acid