title: Bacillus mesophilus sp. nov., an alginate-degrading bacterium isolated from a soil sample collected from an abandoned marine solar saltern.
abstract: A novel Gram-stain positive, endospore-forming bacterium, designated SA4(T), was isolated from a soil sample collected from an abandoned marine solar saltern at Wendeng, Shandong Province, PR China. Cells were observed to be rod shaped, alginase positive, catalase positive and motile. The strain was found to grow at temperatures ranging from 15 to 40 °C (optimum 35 °C), and pH 5.0-11.0 (optimum pH 8.0) with 0-7.0 % (w/v) NaCl concentration (optimum NaCl 3.0 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SA4(T) belongs to the genus Bacillus and exhibits 16S rRNA gene sequence similarities of 96.6, 96.5, 96.3 and 96.2 % with Bacillus horikoshii DSM 8719(T), Bacillus acidicola 105-2(T), Bacillus shackletonii LMG 18435(T) and Bacillus pocheonensis Gsoil 420(T), respectively. The menaquinone was identified as MK-7 and the major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acids detected were anteiso-C15:0 (22.3 %), iso-C15:0 (22.6 %), iso-C16:0 (14.8 %) and iso-C14:0 (14.7 %). The DNA G+C content was determined to be 42.4 mol %. Phenotypic, chemotaxonomic and genotypic properties clearly indicated that isolate SA4(T) represents a novel species within the genus Bacillus, for which the name Bacillus mesophius sp. nov. is proposed. The type strain is SA4(T) (=DSM 101000(T)=CCTCC AB 2015209(T)).
authors.affiliation: College of Marine Science, Shandong University at Weihai, Weihai, 264209, People's Republic of China.
authors.forename: Yan-Xia
authors.initials: YX
authors.lastname: Zhou
authors.affiliation: Agricultural Bio-Resource Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, 350003, People's Republic of China.
authors.forename: Guo-Hong
authors.initials: GH
authors.lastname: Liu
authors.affiliation: Agricultural Bio-Resource Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, 350003, People's Republic of China. fzliubo@163.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.affiliation: College of Marine Science, Shandong University at Weihai, Weihai, 264209, People's Republic of China.
authors.forename: Guan-Jun
authors.initials: GJ
authors.lastname: Chen
authors.affiliation: College of Marine Science, Shandong University at Weihai, Weihai, 264209, People's Republic of China. duzongjun@sdu.edu.cn.
authors.forename: Zong-Jun
authors.initials: ZJ
authors.lastname: Du
mesh_terms: D000464:Alginates / Q000378:metabolism*; D001407:Bacillus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism; D002681:China; D005227:Fatty Acids / Q000378:metabolism; D005838:Genotype; D020723:Glucuronic Acid / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000378:metabolism; D010641:Phenotype; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000378:metabolism
chemical_list: D000464:Alginates; D005227:Fatty Acids; D006603:Hexuronic Acids; D010457:Peptidoglycan; D010714:Phosphatidylethanolamines; D012987:Soil; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; D012965:Sodium Chloride; D020723:Glucuronic Acid
keywords: Alginate-degrading bacterium; Bacillus mesophilus sp. nov.; Marine solar saltern; Soil