title: Inhibition of Lysine-Specific Demethylase-1 (LSD1/KDM1A) Promotes the Adipogenic Differentiation of hESCs Through H3K4 Methylation.
abstract: Given their totipotency, human embryonic stem cells (hESCs) can differentiate into all types of cells, including adipocytes, and provide an excellent research model for studying diseases associated with the metabolism of adipocytes, such as obesity and diabetes mellitus. Epigenetic regulation, including DNA methylation and histone modification, plays an essential role in the development and differentiation of hESCs. Lysine-specific demethylase 1 (LSD1), a well-characterized histone-modifying enzyme, demethylates dimethylated histone H3 lysine 4 (H3K4) through a flavin adenine dinucleotide (FAD)-dependent oxidative reaction. LSD1 affects the growth and differentiation of human and mouse ES cells, and the deletion of this gene in mice leads to embryonic lethality. Here, we investigated the functional role of LSD1 during the adipogenic differentiation of hESCs involving the demethylation of H3K4. We also found that treating hESCs with the LSD1 inhibitor CBB1007 promotes the adipogenic differentiation of hESCs.
authors.affiliation: Reproductive Medical Centre, The First Affiliated Hospital of Zhengzhou University, 1 Jianshe East Road, Zhengzhou, 450052, Henan, People's Republic of China.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Reproductive Medical Centre, The First Affiliated Hospital of Zhengzhou University, 1 Jianshe East Road, Zhengzhou, 450052, Henan, People's Republic of China.
authors.forename: Enyin
authors.initials: E
authors.lastname: Wang
authors.affiliation: Reproductive Medical Centre, The First Affiliated Hospital of Zhengzhou University, 1 Jianshe East Road, Zhengzhou, 450052, Henan, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Reproductive Medical Centre, The First Affiliated Hospital of Zhengzhou University, 1 Jianshe East Road, Zhengzhou, 450052, Henan, People's Republic of China.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Guo
authors.affiliation: Reproductive Medical Centre, The First Affiliated Hospital of Zhengzhou University, 1 Jianshe East Road, Zhengzhou, 450052, Henan, People's Republic of China.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Reproductive Medical Centre, The First Affiliated Hospital of Zhengzhou University, 1 Jianshe East Road, Zhengzhou, 450052, Henan, People's Republic of China.
authors.forename: Qingyun
authors.initials: Q
authors.lastname: Du
authors.affiliation: Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, No. 320 Yueyang Road, Shanghai, 200031, People's Republic of China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Ding
authors.affiliation: Reproductive Medical Centre, The First Affiliated Hospital of Zhengzhou University, 1 Jianshe East Road, Zhengzhou, 450052, Henan, People's Republic of China. syp2008@vip.sina.com.
authors.forename: Yingpu
authors.initials: Y
authors.lastname: Sun
mesh_terms: D017667:Adipocytes / Q000378:metabolism* / Q000502:physiology; D050156:Adipogenesis / Q000502:physiology*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D049109:Cell Proliferation / Q000502:physiology; D019175:DNA Methylation / Q000502:physiology*; D044127:Epigenesis, Genetic / Q000502:physiology; D056466:Histone Demethylases / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D000066449:Human Embryonic Stem Cells / Q000378:metabolism* / Q000502:physiology*; D006801:Humans; D051379:Mice
chemical_list: D006657:Histones; D056466:Histone Demethylases
keywords: Adipogenesis; CBB1007; Histone modification; LSD1; hESCs