title: [Constituents with anti-oxidative activity from seeds of Jufeng grape].
abstract: Taking application of some isolation and purification technologies, including crushing, solvent extraction, preliminary solvent isolation, column chromatographies over silica gel and Sephadex LH-20 gel and preparative HPLC, 8 compounds were obtained from the seeds of Jufeng grape sourced from market. Their structures were identified by spectroscopic methods and comparison with literature values as Catechin (1), Epicatechin (2), quercetin (3), ethylgallate (4), rel-(2S, 3R) -2-(4-hydroxy-3-methoxyphenyl) -3- (hydroxymethyl)-5-(3-hydroxypropyl)-2,3-dihydrobenzofuran-7-ol (5), rel-(2α, 3β)-7-O-methylcedrusin (6), rel-(1R,2S)-1-(4-hydroxy-3-methoxyphenyl) -2-(4-(3-hydroxypropyl) -2-methoxyphenoxy) propane-1,3-diol (7), and (+) -isolariciresinol (8), respectively. Compounds 5-8 were serial lignans isolated from the seeds of grape for the first time. Structurally, 5 and 6 belong in benzofuran-8,3'-neolignans, 7 in 8,4'-oxyneolignan, and 8 in 8,8' :2,7'-cyclolignan. According to in vitro activity evaluation conducted in cell model, compound 6 showed significant anti-oxidative ability, with the activity of RAW264. 7 cell superoxide dismutase being raised evidently in the test as compared with the positive anti-oxidative agents, compounds 1 and 2.
authors.forename: An-jun
authors.initials: AJ
authors.lastname: Deng
authors.forename: Hai-jing
authors.initials: HJ
authors.lastname: Zhang
authors.forename: Zhi-hui
authors.initials: ZH
authors.lastname: Zhang
authors.forename: Zhi-hong
authors.initials: ZH
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Ma
authors.forename: Feng
authors.initials: F
authors.lastname: Wu
authors.forename: Lian-qiu
authors.initials: LQ
authors.lastname: Wu
authors.forename: Wen-jie
authors.initials: WJ
authors.lastname: Wang
authors.forename: Hai-lin
authors.initials: HL
authors.lastname: Qin
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D012639:Seeds / Q000737:chemistry; D027843:Vitis / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts