title: A synthetic biochemistry module for production of bio-based chemicals from glucose.
abstract: Synthetic biochemistry, the cell-free production of biologically based chemicals, is a potentially high-yield, flexible alternative to in vivo metabolic engineering. To limit costs, cell-free systems must be designed to operate continuously with minimal addition of feedstock chemicals. We describe a robust, efficient synthetic glucose breakdown pathway and implement it for the production of bioplastic. The system's performance suggests that synthetic biochemistry has the potential to become a viable industrial alternative.
authors.affiliation: Department of Chemistry and Biochemistry, UCLA-DOE Institute, Molecular Biology Institute, University of California, Los Angeles, Los Angeles, California, USA.
authors.forename: Paul H
authors.initials: PH
authors.lastname: Opgenorth
authors.affiliation: Department of Chemistry and Biochemistry, UCLA-DOE Institute, Molecular Biology Institute, University of California, Los Angeles, Los Angeles, California, USA.
authors.forename: Tyler P
authors.initials: TP
authors.lastname: Korman
authors.affiliation: Department of Chemistry and Biochemistry, UCLA-DOE Institute, Molecular Biology Institute, University of California, Los Angeles, Los Angeles, California, USA.
authors.forename: James U
authors.initials: JU
authors.lastname: Bowie
mesh_terms: D053898:Biosynthetic Pathways; D002474:Cell-Free System; D005947:Glucose / Q000737:chemistry* / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000737:chemistry* / Q000378:metabolism*; D058615:Synthetic Biology / Q000379:methods*
chemical_list: D006885:Hydroxybutyrates; D005947:Glucose