title: Effects of culture conditions on the kinetic behavior of 1,3-propanediol fermentation by Clostridium butyricum with a kinetic model.
abstract: The effects of culture conditions on the kinetic behavior of 1,3-propanediol (PD) fermentation were investigated with a kinetic model. First, with initial glycerol concentration (S0) increasing, μmax and PD inhibition increased. Glycerol assimilation was harder and a little glycerol was consumed on cell maintenance at high S0. Second, with yeast extract concentration increasing, PD inhibition decreased. However, μmax decreased and glycerol assimilation became harder. It seems that the stimulus effect of yeast extract resulted from decreased PD inhibition. Glycerol amount consumed on cell maintenance also decreased. Third, with temperature decreasing, μmax and PD inhibition decreased. Glycerol assimilation was harder and a little more glycerol was consumed on cell maintenance at low temperature. Fourth, with pH increasing, μmax and PD inhibition decreased. Glycerol assimilation was harder and much more glycerol was consumed on cell maintenance at pH 6.5 and 7.5 than 7.0. This work facilitates further fermentation process optimization.
authors.affiliation: School of Biological and Chemical Engineering, Jiangsu Food and Pharmaceutical Science College, Huai'an, Jiangsu 223003, China.
authors.forename: Chunjie
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian 361005, China; The Key Laboratory for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen, Fujian 361005, China; National Engineering Laboratory for Green Chemical Productions of Alcohols-Ethers-Ester, Xiamen University, Xiamen, Fujian 361005, China; The Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen, Fujian 361005, China. Electronic address: fbs@xmu.edu.cn.
authors.forename: Baishan
authors.initials: B
authors.lastname: Fang
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian 361005, China; The Key Laboratory for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen, Fujian 361005, China.
authors.forename: Shizhen
authors.initials: S
authors.lastname: Wang
mesh_terms: D061249:Batch Cell Culture Techniques; D046972:Clostridium butyricum / Q000187:drug effects / Q000378:metabolism*; D003470:Culture Media / Q000494:pharmacology*; D005285:Fermentation / Q000187:drug effects*; D005990:Glycerol / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008962:Models, Theoretical; D011409:Propylene Glycols / Q000378:metabolism*; D013696:Temperature
chemical_list: D003470:Culture Media; D011409:Propylene Glycols; C041787:1,3-propanediol; D005990:Glycerol
keywords: 1,3-Propanediol; Clostridium butyricum; Culture conditions; Kinetic behavior; Kinetic model