title: The BDLF3 gene product of Epstein-Barr virus, gp150, mediates non-productive binding to heparan sulfate on epithelial cells and only the binding domain of CD21 is required for infection.
abstract: The cell surface molecules used by Epstein-Barr virus (EBV) to attach to epithelial cells are not well-defined, although when CD21, the B cell receptor for EBV is expressed epithelial cell infection increases disproportionately to the increase in virus bound. Many herpesviruses use low affinity charge interactions with molecules such as heparan sulfate to attach to cells. We report here that the EBV glycoprotein gp150 binds to heparan sulfate proteoglycans, but that attachment via this glycoprotein is not productive of infection. We also report that only the aminoterminal two short consensus repeats of CD21 are required for efficient infection, This supports the hypothesis that, when expressed on an epithelial cell CD21 serves primarily to cluster the major attachment protein gp350 in the virus membrane and enhance access of other important glycoproteins to the epithelial cell surface.
authors.affiliation: Department of Microbiology and Immunology, Center for Molecular and Tumor Virology and Feist-Weiller Cancer Center, Louisiana State University Health Sciences Center, 1501 Kings Highway, Shreveport, LA 71130, USA. Electronic address: lchesn@lsuhsc.edu.
authors.forename: Liudmila S
authors.initials: LS
authors.lastname: Chesnokova
authors.affiliation: Department of Microbiology and Immunology, Center for Molecular and Tumor Virology and Feist-Weiller Cancer Center, Louisiana State University Health Sciences Center, 1501 Kings Highway, Shreveport, LA 71130, USA. Electronic address: sarah.valencia@nih.gov.
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Valencia
authors.affiliation: Department of Microbiology and Immunology, Center for Molecular and Tumor Virology and Feist-Weiller Cancer Center, Louisiana State University Health Sciences Center, 1501 Kings Highway, Shreveport, LA 71130, USA. Electronic address: lhuttf@lsuhsc.edu.
authors.forename: Lindsey M
authors.initials: LM
authors.lastname: Hutt-Fletcher
mesh_terms: D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D002478:Cells, Cultured; D003412:Cricetulus; D004847:Epithelial Cells / Q000821:virology*; D006497:Heparan Sulfate / Q000378:metabolism*; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D017464:Receptors, Complement 3d / Q000378:metabolism*; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism*; D053585:Virus Attachment
chemical_list: D006497:Heparan Sulfate; D008562:Membrane Glycoproteins; D017464:Receptors, Complement 3d; D014764:Viral Proteins; C109483:BDLF-3 protein, Human herpesvirus 4
keywords: CD21; Epithelial cell infection; Epstein-Barr virus; Glycoprotein 150; Heparan-binding