title: Liquid chromatography with mass spectrometry and NMR spectroscopy based discovery of cytotoxic principles from Daphne tangutica Maxim.
abstract: An ethyl acetate extract from the barks of the ethnic Chinese medicine Daphne tangutica Maxim. exhibited antihepatocellular carcinoma activity against HepG2 and Hep3B cell lines. By using high-performance liquid chromatography based activity profiling in combination with offline liquid chromatography with mass spectrometry and NMR analysis, we rapidly identified ten major components of the extract, including seven active principles, coumarins (1-4) and biscoumarins (7, 8, 10), along with three inactive flavonoids (5, 6, 9). This study demonstrated that our combined protocol can be used as an important strategy for chemical profiling, dereplication as well as the identification of bioactive compounds from herbal medicines.
authors.affiliation: The Modernization Engineering Technology Research Center of Ethnic Minority Medicine of Hubei Province, South-Central University for Nationalities, Wuhan, China.
authors.forename: Xinzhou
authors.initials: X
authors.lastname: Yang
authors.affiliation: The Modernization Engineering Technology Research Center of Ethnic Minority Medicine of Hubei Province, South-Central University for Nationalities, Wuhan, China.
authors.forename: Mi
authors.initials: M
authors.lastname: Huang
authors.affiliation: The Modernization Engineering Technology Research Center of Ethnic Minority Medicine of Hubei Province, South-Central University for Nationalities, Wuhan, China.
authors.forename: Sijian
authors.initials: S
authors.lastname: Zheng
authors.affiliation: The Modernization Engineering Technology Research Center of Ethnic Minority Medicine of Hubei Province, South-Central University for Nationalities, Wuhan, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Ma
authors.affiliation: The Modernization Engineering Technology Research Center of Ethnic Minority Medicine of Hubei Province, South-Central University for Nationalities, Wuhan, China.
authors.forename: Dingrong
authors.initials: D
authors.lastname: Wan
authors.affiliation: Eskitis Institute for Drug Discovery, School of Natural Sciences, Griffith University, Brisbane, Australia.
authors.forename: Yunjiang
authors.initials: Y
authors.lastname: Feng
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D055808:Drug Discovery; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D057809:HEK293 Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D013329:Structure-Activity Relationship
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts
keywords: Activity profiling; Biscoumarins; Cytotoxicity; Daphne tangutica Maxim; Traditional Chinese medicine