title: Proteomic Monitoring of B Cell Immunity.
abstract: Immune monitoring is critical in settings of infection, autoimmunity, and cancer, but our understanding of the diversity of the antibody immune repertoire has been limited to selected target antigens and epitopes. Development of new vaccines requires monitoring of B cell immunity and identification of candidate antigens. As vaccines become more complex, novel techniques are required for monitoring the diversity of the B cell immune response. Since antibodies recognize both linear and conformational protein and glycoprotein epitopes, recent advances in proteomic and glycomic technologies for rapid display of antigenic structures are leading to methods for proteome-wide immune monitoring. Here, we review different approaches for protein display for immune monitoring, and provide methods for in situ protein display for the rapid detection and validation of antibody repertoires.
authors.affiliation: Center for Personalized Diagnostics, School of Life Sciences, The Biodesign Institute, Arizona State University, 876401, Tempe, AZ, 85287, USA.
authors.forename: Radwa
authors.initials: R
authors.lastname: Ewaisha
authors.affiliation: Center for Personalized Diagnostics, School of Life Sciences, The Biodesign Institute, Arizona State University, 876401, Tempe, AZ, 85287, USA. Karen.Anderson.1@asu.edu.
authors.forename: Karen S
authors.initials: KS
authors.lastname: Anderson
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D061505:Cell Surface Display Techniques; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D040901:Proteomics / Q000379:methods*
keywords: Antibody; B cell; Epitope; Immune monitoring; Immunity; Phage display; Protein arrays; Protein display