title: A comparative therapeutic management of anoestrus in buffaloes using insulin and GnRH.
abstract: OBJECTIVE
  Anoestrus is one of the most common functional disorders of the reproductive cycle in buffaloes. In spite of technical advancement, there is no single cure for the management of anoestrus. Therefore, the aim of this study was to find out the efficacy of gonadotropic releasing hormone (GnRH) and metabolic hormone for the management of true anoestrus in buffaloes.
  
  
  METHODS
  The experimental animals were selected on the basis of history, gyneco-clinical examinations and progesterone estimation. Deworming was done with Fenbendazole and thereafter mineral mixture was given @ 50 g per animal per day for 10 days in all the selected buffaloes before the start of treatment. The selected buffaloes were randomly divided into four groups (n=25). In Group I, buffaloes were administered 20 µg of buserelin intramuscularly. Buffaloes of Group II were administered long-acting insulin @ 0.25 IU/Kg body weight subcutaneously for 5 consecutive days. In Group III, buffaloes were treated with a combination of insulin and buserelin in the above-mentioned doses whereas buffaloes of Group IV were kept as untreated control.
  
  
  RESULTS
  The higher oestrus induction (64% vs. 28%) was found in Group III and differed significantly (p<0.05) as compared to control group. The conception rate (69.23% vs. 66.66%) was also found higher in Group III but did not differ significantly among the treated groups. The mean time taken for the onset of oestrus was recorded significantly shorter in insulin (8.80±0.69) and GnRH (7.60±0.92 days) alone and as compared to other (Group III, 14.43±0.83 and Group IV, 20.57±1.69 days) groups.
  
  
  CONCLUSIONS
  The results of this study indicated better fertility response using Insulin plus Buserelin in true anoestrus buffaloes under field conditions.
authors.affiliation: Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science & Animal Husbandry, Tripura, India.
authors.forename: R D
authors.initials: RD
authors.lastname: Purkayastha
authors.affiliation: Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science & Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh, India.
authors.forename: S N
authors.initials: SN
authors.lastname: Shukla
authors.affiliation: Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science & Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh, India.
authors.forename: O P
authors.initials: OP
authors.lastname: Shrivastava
authors.affiliation: Department of Veterinary Gynaecology & Obstetrics, Faculty of Veterinary & Animal Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
authors.forename: P R
authors.initials: PR
authors.lastname: Kumar
keywords: anoestrus; buffalo; buserelin; gonadotropic releasing hormone; insulin