title: Label-free discrimination of different stage nasopharyngeal carcinoma tissue based on Raman spectroscopy.
abstract: The present study aimed to evaluate a label-free tissue test for the detection of nasopharyngeal carcinoma (NPC) at early and advanced stages using Raman spectroscopy (RS). RS measurements were performed to acquire high quality Raman spectra on two groups of tissue samples: One group consists of 30 NPC patients at the early stages (I-II), and the other group is 46 NPC patients at the advanced stages (III-IV). Tentative assignment of Raman bands showed specific biomolecular changes associated with cancer development. Furthermore, effective diagnostic algorithms based on principal components analysis (PCA) and linear discriminant analysis (LDA) were applied for distinguishing Raman spectra of nasopharyngeal tissues from different stages, yielding a diagnostic sensitivity of 70% and a specificity of 78%. This exploratory work suggests that RS in conjunction with the PCA-LDA algorithms provides good diagnostic ability for the early and the advanced staged NPC tissues, and RS has enormous potential for the non-invasive detection of early and advanced stage NPC.
authors.affiliation: The Shengli Clinical Medical College of Fujian Medical University, Fuzhou, Fujian 350001, P.R. China.
authors.forename: Sufang
authors.initials: S
authors.lastname: Qiu
authors.affiliation: The Shengli Clinical Medical College of Fujian Medical University, Fuzhou, Fujian 350001, P.R. China.
authors.forename: Qingting
authors.initials: Q
authors.lastname: Huang
authors.affiliation: The Shengli Clinical Medical College of Fujian Medical University, Fuzhou, Fujian 350001, P.R. China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Huang
authors.affiliation: Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou, Fujian 350007, P.R. China.
authors.forename: Jinyong
authors.initials: J
authors.lastname: Lin
authors.affiliation: Department of Radiation Oncology, Fujian Provincial Cancer Hospital, Fuzhou, Fujian 350014, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lu
authors.affiliation: College of Integrated Traditional Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China.
authors.forename: Duo
authors.initials: D
authors.lastname: Lin
authors.affiliation: Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou, Fujian 350007, P.R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Cao
authors.affiliation: Department of Radiation Biological Laboratory, Fujian Provincial Cancer Hospital, Fuzhou, Fujian 350014, P.R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.affiliation: The Shengli Clinical Medical College of Fujian Medical University, Fuzhou, Fujian 350001, P.R. China; Department of Radiation Oncology, Fujian Provincial Cancer Hospital, Fuzhou, Fujian 350014, P.R. China.
authors.forename: Jianji
authors.initials: J
authors.lastname: Pan
authors.affiliation: Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou, Fujian 350007, P.R. China.
authors.forename: Rong
authors.initials: R
authors.lastname: Chen
keywords: Raman spectroscopy; linear discriminant analysis; nasopharyngeal carcinoma tissue; principle components analysis