title: Prediction of Toxin Genes from Chinese Yellow Catfish Based on Transcriptomic and Proteomic Sequencing.
abstract: Fish venom remains a virtually untapped resource. There are so few fish toxin sequences for reference, which increases the difficulty to study toxins from venomous fish and to develop efficient and fast methods to dig out toxin genes or proteins. Here, we utilized Chinese yellow catfish (Pelteobagrus fulvidraco) as our research object, since it is a representative species in Siluriformes with its venom glands embedded in the pectoral and dorsal fins. In this study, we set up an in-house toxin database and a novel toxin-discovering protocol to dig out precise toxin genes by combination of transcriptomic and proteomic sequencing. Finally, we obtained 15 putative toxin proteins distributed in five groups, namely Veficolin, Ink toxin, Adamalysin, Za2G and CRISP toxin. It seems that we have developed a novel bioinformatics method, through which we could identify toxin proteins with high confidence. Meanwhile, these toxins can also be useful for comparative studies in other fish and development of potential drugs.
authors.affiliation: Section on Marine biobank, China National Genebank, Shenzhen 518083, China. xiebing@genomics.cn.|BGI-Shenzhen, Shenzhen 518083, China. xiebing@genomics.cn.
authors.forename: Bing
authors.initials: B
authors.lastname: Xie
authors.affiliation: Section on Marine biobank, China National Genebank, Shenzhen 518083, China. bgistone@163.com.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Li
authors.affiliation: BGI-Shenzhen, Shenzhen 518083, China. linzhilong@genomics.cn.
authors.forename: Zhilong
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, Shenzhen 518083, China. ruanzhiqiang@genomics.cn.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Ruan
authors.affiliation: Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, Shenzhen 518083, China. wangmin2@genomics.cn.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: BGI-Shenzhen, Shenzhen 518083, China. liujie8@genomics.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: BGI-Shenzhen, Shenzhen 518083, China. tongting@genomics.cn.
authors.forename: Ting
authors.initials: T
authors.lastname: Tong
authors.affiliation: Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, Shenzhen 518083, China. lijia1@genomics.cn.
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.affiliation: Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, Shenzhen 518083, China. huangyu@genomics.cn.
authors.forename: Yu
authors.initials: Y
authors.lastname: Huang
authors.affiliation: BGI-Shenzhen, Shenzhen 518083, China. wenbo@genomics.cn.
authors.forename: Bo
authors.initials: B
authors.lastname: Wen
authors.affiliation: Section on Marine biobank, China National Genebank, Shenzhen 518083, China. ying_sun09@icloud.com.|BGI-Shenzhen, Shenzhen 518083, China. ying_sun09@icloud.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.affiliation: BGI-Shenzhen, Shenzhen 518083, China. shiqiong@genomics.cn.|Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, Shenzhen 518083, China. shiqiong@genomics.cn.|Center for Marine Research, College of Life Sciences, Shenzhen University, Shenzhen 518060, China. shiqiong@genomics.cn.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Shi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002397:Catfishes / Q000235:genetics*; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics*; D020869:Gene Expression Profiling; D023281:Genomics; D008387:Marine Toxins / Q000737:chemistry / Q000235:genetics*; D040901:Proteomics; D059467:Transcriptome
chemical_list: D029941:Fish Proteins; D008387:Marine Toxins
keywords: Chinese yellow catfish; proteome; transcriptome; venom