title: Isolation, analytical measurements, and cell line studies of the iron-bryostatin-1 complex.
abstract: Bryostatin-1 is a marine natural product that has demonstrated medicinal activity in pre-clinical and clinical trials for the treatment of cancer, Alzheimer's disease, effects of stroke, and HIV. In this study, iron-bryostatin-1 was obtained using a pharmaceutical aquaculture technique developed by our lab that cultivates marine bacteria for marine natural product extraction. Analytical measurements (1)H and (13)C NMR, mass spectrometry, and flame atomic absorption were utilized to confirm the presence of an iron-bryostatin-1 complex. The iron-bryostatin-1 complex produced was then tested against the National Cancer Institute's 60 cell line panel. Adding iron to bryostatin-1 lowered the anti-cancer efficacy of the compound.
authors.affiliation: Department of Chemistry, Valdosta State University, Valdosta, GA 31698, United States.
authors.forename: Sydney
authors.initials: S
authors.lastname: Plummer
authors.affiliation: Department of Chemistry, Valdosta State University, Valdosta, GA 31698, United States. Electronic address: tmanning@valdosta.edu.
authors.forename: Thomas
authors.initials: T
authors.lastname: Manning
authors.affiliation: Department of Chemistry, Valdosta State University, Valdosta, GA 31698, United States.
authors.forename: Tess
authors.initials: T
authors.lastname: Baker
authors.affiliation: Department of Chemistry, Valdosta State University, Valdosta, GA 31698, United States.
authors.forename: Tysheon
authors.initials: T
authors.lastname: McGreggor
authors.affiliation: Department of Chemistry, Valdosta State University, Valdosta, GA 31698, United States.
authors.forename: Mehulkumar
authors.initials: M
authors.lastname: Patel
authors.affiliation: NMR Facility, Department of Chemistry, Texas A&M, College Station, TX 77843, United States.
authors.forename: Greg
authors.initials: G
authors.lastname: Wylie
authors.affiliation: PAMS Facility, Department of Chemistry, University of Georgia, Athens, GA 30602, United States.
authors.forename: Dennis
authors.initials: D
authors.lastname: Phillips
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D054713:Bryostatins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D045744:Cell Line, Tumor; D006801:Humans; D007501:Iron / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008828:Microbiological Techniques
chemical_list: D000970:Antineoplastic Agents; D054713:Bryostatins; C046785:bryostatin 1; D007501:Iron
keywords: Aquaculture; Bryostatin; Bugula neritina; Cancer; Candidatus Endobugula sertula; Cell line; Iron; Synthesis