title: Light-induced nuclear export reveals rapid dynamics of epigenetic modifications.
abstract: We engineered a photoactivatable system for rapidly and reversibly exporting proteins from the nucleus by embedding a nuclear export signal in the LOV2 domain from phototropin 1. Fusing the chromatin modifier Bre1 to the photoswitch, we achieved light-dependent control of histone H2B monoubiquitylation in yeast, revealing fast turnover of the ubiquitin mark. Moreover, this inducible system allowed us to dynamically monitor the status of epigenetic modifications dependent on H2B ubiquitylation.
authors.affiliation: Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina, USA.
authors.forename: Hayretin
authors.initials: H
authors.lastname: Yumerefendi
authors.affiliation: Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina, USA.
authors.forename: Andrew Michael
authors.initials: AM
authors.lastname: Lerner
authors.affiliation: Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina, USA.
authors.forename: Seth Parker
authors.initials: SP
authors.lastname: Zimmerman
authors.affiliation: Department of Pharmacology, University of North Carolina, Chapel Hill, North Carolina, USA.|Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina, USA.
authors.forename: Klaus
authors.initials: K
authors.lastname: Hahn
authors.affiliation: Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina, USA.|Department of Cell Biology &Physiology, University of North Carolina, Chapel Hill, North Carolina, USA.
authors.forename: James E
authors.initials: JE
authors.lastname: Bear
authors.affiliation: Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina, USA.|Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina, USA.
authors.forename: Brian D
authors.initials: BD
authors.lastname: Strahl
authors.affiliation: Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina, USA.|Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina, USA.
authors.forename: Brian
authors.initials: B
authors.lastname: Kuhlman
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism* / Q000528:radiation effects*; D044127:Epigenesis, Genetic / Q000528:radiation effects*; D005420:Flavoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D008027:Light; D049790:Nuclear Export Signals; D056949:Phototropins / Q000737:chemistry; D000072417:Protein Domains; D021381:Protein Transport / Q000528:radiation effects; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D025801:Ubiquitin / Q000378:metabolism; D054875:Ubiquitination / Q000528:radiation effects*
chemical_list: C472168:Bre1 protein, S cerevisiae; D005420:Flavoproteins; D006657:Histones; D049790:Nuclear Export Signals; D056949:Phototropins; D029701:Saccharomyces cerevisiae Proteins; D025801:Ubiquitin