title: Characterization of the contribution of buccal absorption to internal exposure to bisphenol A through the diet.
abstract: The gavage route is often used for the toxicological evaluation of food contaminants. This route does not take into account absorption of the toxicants through the buccal mucosa, as evidenced in dogs for bisphenol A (BPA). Our goal was to determine the functional significance of buccal BPA absorption during dietary exposure. Four ewes received BPA by nasogastric gavage (100 mg/kg) and through food pellets (10 mg/kg), 13 days apart. The time course of serum concentrations of BPA and its main metabolite BPA-G was submitted to non-compartmental analysis. The dietary route led to 3-fold higher bioavailability as compared to gavage. The ratio of BPA-G to BPA concentrations varied greatly over time after the food administration, but not after gavage, suggesting a delayed metabolism of BPA after dietary exposure. The maximum entrance rate of BPA in the systemic circulation, determined by deconvolution analysis, was much higher after dietary administration than after gavage and a biphasic pattern of BPA entry was observed in 3 of the 4 ewes. Our results evidenced a dual mechanism of BPA absorption (buccal and digestive) after dietary exposure and highlight the necessity to take buccal absorption into account when evaluating food contaminants.
authors.affiliation: Toxalim, Université de Toulouse, INRA, INP-ENVT, INP-EI-Purpan, Université de Toulouse 3 Paul Sabatier, Toulouse, France.
authors.forename: Davy
authors.initials: D
authors.lastname: Guignard
authors.affiliation: Toxalim, Université de Toulouse, INRA, INP-ENVT, INP-EI-Purpan, Université de Toulouse 3 Paul Sabatier, Toulouse, France.
authors.forename: Glenn
authors.initials: G
authors.lastname: Gauderat
authors.affiliation: Toxalim, Université de Toulouse, INRA, INP-ENVT, INP-EI-Purpan, Université de Toulouse 3 Paul Sabatier, Toulouse, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Gayrard
authors.affiliation: Toxalim, Université de Toulouse, INRA, INP-ENVT, INP-EI-Purpan, Université de Toulouse 3 Paul Sabatier, Toulouse, France.
authors.forename: Marlène Z
authors.initials: MZ
authors.lastname: Lacroix
authors.affiliation: Toxalim, Université de Toulouse, INRA, INP-ENVT, INP-EI-Purpan, Université de Toulouse 3 Paul Sabatier, Toulouse, France.
authors.forename: Nicole
authors.initials: N
authors.lastname: Picard-Hagen
authors.affiliation: Toxalim, Université de Toulouse, INRA, INP-ENVT, INP-EI-Purpan, Université de Toulouse 3 Paul Sabatier, Toulouse, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Puel
authors.affiliation: Toxalim, Université de Toulouse, INRA, INP-ENVT, INP-EI-Purpan, Université de Toulouse 3 Paul Sabatier, Toulouse, France.
authors.forename: Pierre-Louis
authors.initials: PL
authors.lastname: Toutain
authors.affiliation: Toxalim, Université de Toulouse, INRA, INP-ENVT, INP-EI-Purpan, Université de Toulouse 3 Paul Sabatier, Toulouse, France. Electronic address: catherine.viguie@toulouse.inra.fr.
authors.forename: Catherine
authors.initials: C
authors.lastname: Viguié
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000493:pharmacokinetics* / Q000494:pharmacology; D004032:Diet; D004285:Dogs; D016166:Free Radical Scavengers / Q000493:pharmacokinetics* / Q000494:pharmacology; D009061:Mouth Mucosa / Q000187:drug effects / Q000378:metabolism*; D065568:Oral Mucosal Absorption / Q000187:drug effects*; D010636:Phenols / Q000493:pharmacokinetics* / Q000494:pharmacology; D012756:Sheep; D014018:Tissue Distribution; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D016166:Free Radical Scavengers; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Buccal absorption; Food toxicology; Pharmacokinetic