title: Development of Structure-Based Vaccines for Ehrlichiosis.
abstract: The obligate intracellular bacterium Ehrlichia chaffeensis that resides in mononuclear phagocytes is the etiologic agent of human monocytotropic ehrlichiosis (HME). HME is an emerging and often life-threatening zoonotic, tick-transmitted infectious disease in the USA. Lack of early diagnosis and treatment of HME are the main factors that lead to severe and fatal disease. Ehrlichia also causes diseases in companion animals and domesticated ruminants. E. chaffeensis and E. canis cause canine ehrlichioses in dogs, whereas E. ruminantium causes heartwater in cattle, sheep, and goats. As yet there are no commercially available vaccines to protect against these pathogens. This chapter describes a protocol to develop a structure-based vaccine for Ehrlichia based on the antigenic proteins P28-19 and Ehrlichia Hsp60.
authors.affiliation: Lankenau Institute for Medical Research, Wynnewood, PA, 19096, USA. suntom2@gmail.com.
authors.forename: Sunil
authors.initials: S
authors.lastname: Thomas
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology; D001428:Bacterial Vaccines / Q000737:chemistry* / Q000276:immunology*; D015153:Blotting, Western; D002460:Cell Line; D018834:Chaperonin 60 / Q000737:chemistry / Q000276:immunology; D015195:Drug Design; D004536:Ehrlichia / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D007114:Immunization; D051379:Mice; D008853:Microscopy
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D018834:Chaperonin 60
keywords: Diagnostics; Eastern blotting; Ehrlichia; Ehrlichiosis; Rickettsiales; Structure-based vaccines; Vaccine