title: The flagellin hypervariable region is a potential flagella display domain in probiotic Escherichia coli strain Nissle 1917.
abstract: The most studied probiotic, Escherichia coli strain Nissle 1917 (EcN) possesses flagella of serotype H1. To explore the potential to use EcN flagellin in flagella display applications, we investigated the effect of deleting amino acids in the hypervariable region of flagellin on EcNc (EcN cured of its two cryptic plasmids pMUT1 and pMUT2). Two EcNc flagellin isogenic mutants with deletions of amino acid residual from 277 to 286 and from 287 to 296 in the hypervariable domain were constructed. Both mutants were flagellated, adherent to IPEC-J2 cells, and colonized BALB/c mice. These hypervariable regions may have future utility in the display of heterologous epitopes.
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu, People's Republic of China.|Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu, People's Republic of China.|Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu, People's Republic of China.|Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, People's Republic of China.
authors.forename: Bingming
authors.initials: B
authors.lastname: Ou
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu, People's Republic of China.|Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, People's Republic of China.
authors.forename: Pengpeng
authors.initials: P
authors.lastname: Xia
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu, People's Republic of China.|Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, People's Republic of China.
authors.forename: Mingxu
authors.initials: M
authors.lastname: Zhou
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu, People's Republic of China.|Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, People's Republic of China.|Faculty of Land and Food Systems, University of British Columbia, Vancouver, Canada.
authors.forename: Luan
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu, People's Republic of China. yzgqzhu@yzu.edu.cn.|Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, People's Republic of China. yzgqzhu@yzu.edu.cn.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000235:genetics*; D002460:Cell Line; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005407:Flagella / Q000378:metabolism*; D005408:Flagellin / Q000235:genetics* / Q000378:metabolism; D017353:Gene Deletion; D051379:Mice; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D010957:Plasmids / Q000235:genetics; D019936:Probiotics; D017434:Protein Structure, Tertiary; D013552:Swine
chemical_list: D029968:Escherichia coli Proteins; C516095:FliC protein, E coli; D005408:Flagellin
keywords: E. coli strain Nissle 1917; Flagella display application; Flagellin