title: Role of Sinomenine on Complete Freund's Adjuvant-Induced Arthritis in Rats.
abstract: The investigation was undertaken to evaluate the effect of sinomenine (Sin) on experimental adjuvant arthritis rats stimulated by Freund's complete adjuvant and explore the corresponding potential molecular mechanism. The content of proinflammatory cytokines such as tumor necrosis factor-alpha, interleukin-1 beta and interleukin-6 were detected. Besides, canonical nuclear factor kappa B (NF-κB) pathway was also assessed to evaluate the antiarthritic potential of sinomenine. Pathological sections of rat paws showed sinomenine and diclofenac sodium significantly alleviated articular cartilage lesion, cellular infiltration, epithelial cell degeneration, synovial tissue vasodilation and congestion. The phosphorylations of inhibitor of kappaB alpha and NF-κB subunit p65 were downregulated with the treatment of sinomenine in dose dependent manners, as well as proinflammatory cytokines. Therefore, it was assumed that sinomenine might be a new therapeutic candidate to treat arthritis. © 2016 IUBMB Life, 68(6):429-435, 2016.
authors.affiliation: School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, People's Republic of China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Lan
authors.affiliation: School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, People's Republic of China.
authors.forename: Meng
authors.initials: M
authors.lastname: Wei
authors.affiliation: School of Pharmacy, South-Central University for Nationalities, Wuhan, People's Republic of China.
authors.forename: Lvyi
authors.initials: L
authors.lastname: Chen
authors.affiliation: School of Basic Medicine, Hubei University of Chinese Medicine, Wuhan, People's Republic of China.
authors.forename: Guangjing
authors.initials: G
authors.lastname: Xie
authors.affiliation: School of Basic Medicine, Hubei University of Chinese Medicine, Wuhan, People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.affiliation: School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, People's Republic of China.
authors.forename: Xiuqiao
authors.initials: X
authors.lastname: Zhang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001169:Arthritis, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism*; D016207:Cytokines / Q000097:blood; D004487:Edema / Q000139:chemically induced; D005620:Freund's Adjuvant / Q000633:toxicity; D020672:I-kappa B Proteins / Q000378:metabolism; D009019:Morphinans / Q000494:pharmacology*; D009687:Nuclear Proteins / Q000378:metabolism; D000078723:Nuclear Receptor Interacting Protein 1; D010766:Phosphorylation / Q000187:drug effects; D017207:Rats, Sprague-Dawley; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000894:Anti-Inflammatory Agents, Non-Steroidal; D016207:Cytokines; D020672:I-kappa B Proteins; D009019:Morphinans; D009687:Nuclear Proteins; D000078723:Nuclear Receptor Interacting Protein 1; C498145:Rela protein, rat; D051996:Transcription Factor RelA; C009271:sinomenine; D005620:Freund's Adjuvant
keywords: RIP140; arthritis; nuclear factor kappa B; sinomenine