title: Pyrroloquinoline quinone enhances the resistance to oxidative stress and extends lifespan upon DAF-16 and SKN-1 activities in C. elegans.
abstract: Pyrroloquinoline quinone (PQQ) is linked to fundamental biological processes such as mitochondrial biogenesis and lipid metabolism. PQQ may also function as an essential micronutrient during animal development. Recent studies have shown the therapeutic potential of PQQ for several age-related diseases due to its antioxidant capacity. However, whether PQQ can promote longevity is unknown. Here, we investigate the effects of PQQ on oxidative stress resistance as well as lifespan modulation in Caenorhabditis elegans. We find that PQQ enhances resistance to oxidative stress and extends the lifespan of C. elegans at optimal doses. The underlying molecular mechanism involves the increased activities of the primary lifespan extension transcriptional factors DAF-16/FOXO, the conserved oxidative stress-responsive transcription factor SKN-1/Nrf2, and upregulation of daf-16, skn-1 downstream targets including sod-3, hsp16.2, gst-1 and gst-10. Our findings uncover a novel role of PQQ in longevity, supporting PQQ as a possible dietary supplement for overall health improvement.
authors.affiliation: Department of Geriatrics, Shanghai Institute of Geriatrics, Huadong Hospital, Fudan University, Shanghai 200040, China; Institute of Integrated Traditional Chinese and Medicine and Western Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: J Z
authors.initials: JZ
authors.lastname: Wu
authors.affiliation: Institute of Integrated Traditional Chinese and Medicine and Western Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: J H
authors.initials: JH
authors.lastname: Huang
authors.affiliation: Department of Maternal-Fetal Medicine Pregnancy Research Centre, University of Melbourne Department of Obstetrics and Gynaecology, Royal Women's Hospital, Parkville 3052, Australia.
authors.forename: R
authors.initials: R
authors.lastname: Khanabdali
authors.affiliation: Department of Maternal-Fetal Medicine Pregnancy Research Centre, University of Melbourne Department of Obstetrics and Gynaecology, Royal Women's Hospital, Parkville 3052, Australia.
authors.forename: B
authors.initials: B
authors.lastname: Kalionis
authors.affiliation: Department of Geriatrics, Shanghai Institute of Geriatrics, Huadong Hospital, Fudan University, Shanghai 200040, China. Electronic address: xiashijinhd@163.com.
authors.forename: S J
authors.initials: SJ
authors.lastname: Xia
authors.affiliation: Institute of Integrated Traditional Chinese and Medicine and Western Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China. Electronic address: caiwaijiao@126.com.
authors.forename: W J
authors.initials: WJ
authors.lastname: Cai
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D051858:Forkhead Transcription Factors / Q000235:genetics; D008136:Longevity / Q000502:physiology*; D018384:Oxidative Stress; D045542:PQQ Cofactor / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics
chemical_list: D029742:Caenorhabditis elegans Proteins; D004268:DNA-Binding Proteins; D051858:Forkhead Transcription Factors; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D014157:Transcription Factors; C108831:daf-16 protein, C elegans; C073702:skn-1 protein, C elegans; D045542:PQQ Cofactor; C525107:Sod-3 protein, C elegans; D013482:Superoxide Dismutase
keywords: C. elegans; IIS signalling; Lifespan; Oxidative stress; Pyrroloquinoline quinone