title: Use Dependence of Heat Sensitivity of Vanilloid Receptor TRPV2.
abstract: Thermal TRP channels mediate temperature transduction and pain sensation. The vanilloid receptor TRPV2 is involved in detection of noxious heat in a subpopulation of high-threshold nociceptors. It also plays a critical role in development of thermal hyperalgesia, but the underlying mechanism remains uncertain. Here we analyze the heat sensitivity of the TRPV2 channel. Heat activation of the channel exhibits strong use dependence. Prior heat activation can profoundly alter its subsequent temperature responsiveness, causing decreases in both temperature activation threshold and slope sensitivity of temperature dependence while accelerating activation time courses. Notably, heat and agonist activations differ in cross use-dependence. Prior heat stimulation can dramatically sensitize agonist responses, but not conversely. Quantitative analyses indicate that the use dependence in heat sensitivity is pertinent to the process of temperature sensing by the channel. The use dependence of TRPV2 reveals that the channel can have a dynamic temperature sensitivity. The temperature sensing structures within the channel have multiple conformations and the temperature activation pathway is separate from the agonist activation pathway. Physiologically, the use dependence of TRPV2 confers nociceptors with a hypersensitivity to heat and thus provides a mechanism for peripheral thermal hyperalgesia.
authors.affiliation: Department of Physiology/Biophysics, State University of New York-Buffalo, Buffalo, New York.
authors.forename: Beiying
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Physiology/Biophysics, State University of New York-Buffalo, Buffalo, New York. Electronic address: qin@buffalo.edu.
authors.forename: Feng
authors.initials: F
authors.lastname: Qin
mesh_terms: D000818:Animals; D057809:HEK293 Cells; D006358:Hot Temperature; D006801:Humans; D007700:Kinetics; D051381:Rats; D050916:TRPV Cation Channels / Q000819:agonists / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D050916:TRPV Cation Channels; C476722:Trpv2 protein, rat