title: Gene set of chemosensory receptors in the polyembryonic endoparasitoid Macrocentrus cingulum.
abstract: Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system. The main chemosensory organ, antennae play a critical role in detecting odor in ambient environment before initiating appropriate behavioral responses. The antennal chemosensory receptor genes families have been suggested to be involved in olfactory signal transduction pathway as a sensory neuron response. The Macrocentrus cingulum is deployed successfully as a biological control agent for corn pest insects from the Lepidopteran genus Ostrinia. In this research, we assembled antennal transcriptomes of M. cingulum by using next generation sequencing to identify the major chemosensory receptors gene families. In total, 112 olfactory receptors candidates (79 odorant receptors, 20 gustatory receptors, and 13 ionotropic receptors) have been identified from the male and female antennal transcriptome. The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing. Expression profiles of gustatory receptors in olfactory and non-olfactory tissues were measured by RT-qPCR. The sex-specific and sex-biased chemoreceptors expression patterns suggested that they may have important functions in sense detection which behaviorally relevant to odor molecules. This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.
authors.affiliation: State Key Laboratory for the Biology of the Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.|Bangladesh Sugarcrop Research Institute, Ishurdi-6620, Pabna, Bangladesh.
authors.forename: Tofael
authors.initials: T
authors.lastname: Ahmed
authors.affiliation: State Key Laboratory for the Biology of the Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Tiantao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for the Biology of the Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Zhenying
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory for the Biology of the Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Kanglai
authors.initials: K
authors.lastname: He
authors.affiliation: State Key Laboratory for the Biology of the Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Shuxiong
authors.initials: S
authors.lastname: Bai
mesh_terms: D000818:Animals; D057506:Arthropod Antennae / Q000502:physiology; D005260:Female; D005786:Gene Expression Regulation; D059014:High-Throughput Nucleotide Sequencing; D006927:Hymenoptera / Q000235:genetics* / Q000502:physiology; D007915:Lepidoptera / Q000235:genetics* / Q000469:parasitology; D008297:Male; D010572:Pest Control, Biological; D018035:Receptors, Odorant / Q000096:biosynthesis / Q000235:genetics*; D015398:Signal Transduction; D059467:Transcriptome / Q000235:genetics*; D003313:Zea mays / Q000254:growth & development / Q000469:parasitology
chemical_list: D018035:Receptors, Odorant