title: SBP2 plays an important role in the virulence changes of different artificial mutants of Streptococcus suis.
abstract: Streptococcus suis (SS) is an important bacterial zoonotic pathogen, which can cause infections in pigs and humans. However, the pathogenesis of this bacterium remains unclear, even though some putative virulence factors (VFs) have been reported. Comparative proteomics could be used to identify markers that can distinguish bacterial strains with different virulence; however, the application of this method is restricted by the genome diversities existing in different strains. In this study, two mutants, WT ΔpepT and WT ΔrfeA, which were generated from the same wild-type (WT) strain, ZY05719, and showed opposite virulence tendencies, were constructed. Combining two proteomics assays, two-dimensional difference gel electrophoresis (2D-DIGE) and label-free proteomics, we identified 38 differentially abundant proteins in the mutants compared with their parent, including five known VFs of S. suis and 33 novel elements. One of the novel proteins, a putative pilus protein, named SBP2, was considered as the most promising VF, because SBP2 was not only linked with the known VFs in the virulence interaction network and was proposed to be located on the cell surface, but also showed enriched distribution among highly virulent strains of SS. SBP2 could also bind fibronectin and laminin, two important extracellular matrix proteins of the host, to facilitate the process of adhesion. Thus, spb2 was identified as encoding a promising virulence-associated candidate associated with the pathogenesis of SS, and a comprehensive virulence interaction network of SS was established for the first time.
authors.affiliation: OIE Swine Streptococcosis Diagnostic Laboratory & Key Lab of Animal Bacteriology of Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China. 2013207020@njau.edu.cn 2015107079@njau.edu.cn 2012107079@njau.edu.cn 2014107079@njau.edu.cn 2012107080@njau.edu.cn 2013107047@njau.edu.cn shaojing0927@126.com wuzongfu@njau.edu.cn vszw@njau.edu.cn.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Yu
authors.affiliation: OIE Swine Streptococcosis Diagnostic Laboratory & Key Lab of Animal Bacteriology of Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China. 2013207020@njau.edu.cn 2015107079@njau.edu.cn 2012107079@njau.edu.cn 2014107079@njau.edu.cn 2012107080@njau.edu.cn 2013107047@njau.edu.cn shaojing0927@126.com wuzongfu@njau.edu.cn vszw@njau.edu.cn.
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Qian
authors.affiliation: OIE Swine Streptococcosis Diagnostic Laboratory & Key Lab of Animal Bacteriology of Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China. 2013207020@njau.edu.cn 2015107079@njau.edu.cn 2012107079@njau.edu.cn 2014107079@njau.edu.cn 2012107080@njau.edu.cn 2013107047@njau.edu.cn shaojing0927@126.com wuzongfu@njau.edu.cn vszw@njau.edu.cn.
authors.forename: Dechao
authors.initials: D
authors.lastname: Du
authors.affiliation: OIE Swine Streptococcosis Diagnostic Laboratory & Key Lab of Animal Bacteriology of Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China. 2013207020@njau.edu.cn 2015107079@njau.edu.cn 2012107079@njau.edu.cn 2014107079@njau.edu.cn 2012107080@njau.edu.cn 2013107047@njau.edu.cn shaojing0927@126.com wuzongfu@njau.edu.cn vszw@njau.edu.cn.
authors.forename: Chenyang
authors.initials: C
authors.lastname: Xu
authors.affiliation: Laboratory Center of Life Sciences, College of Life Science, Nanjing Agricultural University, Nanjing, China. daichencpu@njau.edu.cn.
authors.forename: Chen
authors.initials: C
authors.lastname: Dai
authors.affiliation: OIE Swine Streptococcosis Diagnostic Laboratory & Key Lab of Animal Bacteriology of Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China. 2013207020@njau.edu.cn 2015107079@njau.edu.cn 2012107079@njau.edu.cn 2014107079@njau.edu.cn 2012107080@njau.edu.cn 2013107047@njau.edu.cn shaojing0927@126.com wuzongfu@njau.edu.cn vszw@njau.edu.cn.
authors.forename: Quan
authors.initials: Q
authors.lastname: Li
authors.affiliation: OIE Swine Streptococcosis Diagnostic Laboratory & Key Lab of Animal Bacteriology of Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China. 2013207020@njau.edu.cn 2015107079@njau.edu.cn 2012107079@njau.edu.cn 2014107079@njau.edu.cn 2012107080@njau.edu.cn 2013107047@njau.edu.cn shaojing0927@126.com wuzongfu@njau.edu.cn vszw@njau.edu.cn.
authors.forename: Hanze
authors.initials: H
authors.lastname: Liu
authors.affiliation: OIE Swine Streptococcosis Diagnostic Laboratory & Key Lab of Animal Bacteriology of Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China. 2013207020@njau.edu.cn 2015107079@njau.edu.cn 2012107079@njau.edu.cn 2014107079@njau.edu.cn 2012107080@njau.edu.cn 2013107047@njau.edu.cn shaojing0927@126.com wuzongfu@njau.edu.cn vszw@njau.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Shao
authors.affiliation: OIE Swine Streptococcosis Diagnostic Laboratory & Key Lab of Animal Bacteriology of Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China. 2013207020@njau.edu.cn 2015107079@njau.edu.cn 2012107079@njau.edu.cn 2014107079@njau.edu.cn 2012107080@njau.edu.cn 2013107047@njau.edu.cn shaojing0927@126.com wuzongfu@njau.edu.cn vszw@njau.edu.cn.
authors.forename: Zongfu
authors.initials: Z
authors.lastname: Wu
authors.affiliation: OIE Swine Streptococcosis Diagnostic Laboratory & Key Lab of Animal Bacteriology of Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China. 2013207020@njau.edu.cn 2015107079@njau.edu.cn 2012107079@njau.edu.cn 2014107079@njau.edu.cn 2012107080@njau.edu.cn 2013107047@njau.edu.cn shaojing0927@126.com wuzongfu@njau.edu.cn vszw@njau.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D019295:Computational Biology / Q000379:methods; D030562:Databases, Protein; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D017353:Gene Deletion; D005786:Gene Expression Regulation; D055786:Gene Knockout Techniques; D063990:Gene Ontology; D018390:Gene Targeting; D056426:Genetic Loci; D006801:Humans; D009154:Mutation; D011485:Protein Binding; D025941:Protein Interaction Mapping; D060066:Protein Interaction Maps; D040901:Proteomics / Q000379:methods; D013290:Streptococcal Infections / Q000382:microbiology*; D017011:Streptococcus suis / Q000145:classification / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics*; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism; D015027:Zebrafish
chemical_list: D001426:Bacterial Proteins; D016326:Extracellular Matrix Proteins; D037521:Virulence Factors