title: miR-128 regulates differentiation of hair follicle mesenchymal stem cells into smooth muscle cells by targeting SMAD2.
abstract: Human hair follicle mesenchymal stem cells (hHFMSCs) are an important source of cardiovascular tissue engineering for their differentiation potential into smooth muscle cells (SMCs), yet the molecular pathways underlying such fate determination is unclear. MicroRNAs (miRNAs) are non-coding RNAs that play critical roles in cell differentiation. In present study, we found that miR-128 was remarkably decreased during the differentiation of hHFMSCs into SMCs induced by transforming growth factor-β1 (TGF-β1). Moreover, overexpression of miR-128 led to decreased expression of SMC cellular marker proteins, such as smooth muscle actin (SMA) and calponin, in TGF-β1-induced SMC differentiation. Further, we identified that miR-128 targeted the 3'-UTR of SMAD2 transcript for translational inhibition of SMAD2 protein, and knockdown of SMAD2 abrogated the promotional effect of antagomir-128 (miR-128 neutralizer) on SMC differentiation. These results suggest that miR-128 regulates the differentiation of hHFMSCs into SMCs via targeting SMAD2, a main transcription regulator in TGF-β signaling pathway involving SMC differentiation. The miR-128/SMAD2 axis could therefore be considered as a candidate target in tissue engineering and regenerative medicine for SMCs.
authors.affiliation: Department of Geriatrics, The First Bethune Hospital of Jilin University, Changchun, Jilin Province, 130021, People's Republic of China. Electronic address: wangzhih@jlu.edu.cn.
authors.forename: Zhihao
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Emergency Medicine, The First Bethune Hospital of Jilin University, Changchun, Jilin Province, 130021, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Pang
authors.affiliation: Department of Geriatrics, The First Bethune Hospital of Jilin University, Changchun, Jilin Province, 130021, People's Republic of China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Respiratory Medicine, The First Bethune Hospital of Jilin University, Changchun, Jilin Province, 130021, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Song
authors.affiliation: Department of Geriatrics, The First Bethune Hospital of Jilin University, Changchun, Jilin Province, 130021, People's Republic of China.
authors.forename: Yuehui
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Diagnostic and Treatment Center for Cardiovascular Diseases, The First Bethune Hospital of Jilin University, Changchun, Jilin Province, 130021, People's Republic of China; Department of Cardiology, The 202 Hospital of PLA, Shenyang, Liaoning Province, 110003, People's Republic of China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Department of Radiology, The First Bethune Hospital of Jilin University, Changchun, Jilin Province, 130021, People's Republic of China.
authors.forename: Chunjie
authors.initials: C
authors.lastname: Guo
authors.affiliation: Department of Infectious Diseases, The First Bethune Hospital of Jilin University, Changchun, Jilin Province, 130021, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Geriatrics, The First Bethune Hospital of Jilin University, Changchun, Jilin Province, 130021, People's Republic of China.
authors.forename: Xiujiao
authors.initials: X
authors.lastname: Qin
authors.affiliation: Diagnostic and Treatment Center for Cardiovascular Diseases, The First Bethune Hospital of Jilin University, Changchun, Jilin Province, 130021, People's Republic of China. Electronic address: panaq@jlu.edu.cn.
authors.forename: Aiqun
authors.initials: A
authors.lastname: Pan
mesh_terms: D020413:3' Untranslated Regions; D001483:Base Sequence; D001665:Binding Sites; D002454:Cell Differentiation; D002478:Cells, Cultured; D018859:Hair Follicle / Q000166:cytology*; D006801:Humans; D007231:Infant, Newborn; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D035683:MicroRNAs / Q000235:genetics*; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D034622:RNA Interference; D051899:Smad2 Protein / Q000235:genetics* / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000502:physiology
chemical_list: D020413:3' Untranslated Regions; C545189:MIRN128 microRNA, human; D035683:MicroRNAs; C497782:SMAD2 protein, human; D051899:Smad2 Protein; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1
keywords: Calponin; Differentiation; SMAD2; Smooth muscle cell; TGF-β; miR-128