title: Changes of Mouse Gut Microbiota Diversity and Composition by Modulating Dietary Protein and Carbohydrate Contents: A Pilot Study.
abstract: Dietary proteins influence colorectal cancer (CRC) risk, depending on their quantity and quality. Here, using pyrosequencing, we compared the fecal microbiota composition in Balb/c mice fed either a normal protein/carbohydrate diet (ND, 20% casein and 68% carbohydrate) or a high-protein/low-carbohydrate diet (HPLCD, 30% casein and 57% carbohydrate). The results showed that HPLCD feeding for 2 weeks reduced the diversity and altered the composition of the microbiota compared with the ND mice, which included a decrease in the proportion of the family Lachnospiraceae and Ruminococcaceae and increases in the proportions of the genus Bacteroides and Parabacteroides, especially the species EF09600_s and EF604598_s. Similar changes were reported in patients with inflammatory bowel disease, and in mouse models of CRC and colitis, respectively. This suggests that HPLCD may lead to a deleterious luminal environment and may have adverse effects on the intestinal health of individuals consuming such a diet.
authors.affiliation: Department of Food Science and Nutrition, Catholic University of Daegu, Gyeongbuk 38430, Korea.
authors.forename: Eunjung
authors.initials: E
authors.lastname: Kim
authors.affiliation: Department of Food Science and Nutrition, Catholic University of Daegu, Gyeongbuk 38430, Korea.
authors.forename: Dan-Bi
authors.initials: DB
authors.lastname: Kim
authors.affiliation: Department of Food Science and Nutrition, Catholic University of Daegu, Gyeongbuk 38430, Korea.
authors.forename: Jae-Yong
authors.initials: JY
authors.lastname: Park
keywords: gut microbiota; high protein diet; low carbohydrate diet; mouse; pyrosequencing