title: Structural and Nonstructural Viral Proteins Are Targets of T-Helper Immune Response against Human Respiratory Syncytial Virus.
abstract: Proper antiviral humoral and cellular immune responses require previous recognition of viral antigenic peptides that are bound to HLA class II molecules, which are exposed on the surface of antigen-presenting cells. The helper immune response is critical for the control and the clearance of human respiratory syncytial virus (HRSV) infection, a virus with severe health risk in infected pediatric, immunocompromised, and elderly populations. In this study, using a mass spectrometry analysis of complex HLA class II-bound peptide pools that were isolated from large amounts of HRSV-infected cells, 19 naturally processed HLA-DR ligands, most of them included in a complex nested set of peptides, were identified. Both the immunoprevalence and the immunodominance of the HLA class II response to HRSV were focused on one nonstructural (NS1) and two structural (matrix and mainly fusion) proteins of the infective virus. These findings have clear implications for analysis of the helper immune response as well as for antiviral vaccine design.
authors.affiliation: From the ‡Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda (Madrid), Spain.
authors.forename: Elena
authors.identifier: 0000-0002-2246-6851
authors.initials: E
authors.lastname: Lorente
authors.affiliation: From the ‡Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda (Madrid), Spain.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Barriga
authors.affiliation: §Department of Biology, Technion-Israel Institute of Technology, 32000 Haifa, Israel.
authors.forename: Eilon
authors.initials: E
authors.lastname: Barnea
authors.affiliation: From the ‡Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda (Madrid), Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Mir
authors.affiliation: ¶Benaroya Research Institute, Seattle, WA 98101, USA.
authors.forename: John A
authors.initials: JA
authors.lastname: Gebe
authors.affiliation: §Department of Biology, Technion-Israel Institute of Technology, 32000 Haifa, Israel.
authors.forename: Arie
authors.initials: A
authors.lastname: Admon
authors.affiliation: From the ‡Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda (Madrid), Spain, dlopez@isciii.es.
authors.forename: Daniel
authors.identifier: 0000-0003-0268-5878
authors.initials: D
authors.lastname: López
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D018360:Crystallography, X-Ray; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D006801:Humans; D007111:Immunity, Cellular; D016131:Lymphocyte Subsets / Q000276:immunology; D051379:Mice; D010455:Peptides / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D018113:Respiratory Syncytial Virus, Human / Q000276:immunology* / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D017361:Viral Nonstructural Proteins / Q000276:immunology*; D015678:Viral Structural Proteins / Q000276:immunology*
chemical_list: D000949:Histocompatibility Antigens Class II; D010455:Peptides; D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins