title: DNA Integration in Leishmania Genome: An Application for Vaccine Development and Drug Screening.
abstract: Transfection technology is an important tool in the investigation of gene function and the modulation of gene expression, thereby contributing to the advancement of basic cellular research, drug discovery, and target validation. Creation of the mutant cells through gene disruption and exogenous protein expression with noticeable phenotype like reporter genes are among other key applications. In this chapter, protocols for generating recombinant Leishmania expressing EGFP or EGFP-Luciferase and their applications are given in detail.
authors.affiliation: Department of Immunotherapy and Leishmania Vaccine Research, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Tahereh
authors.initials: T
authors.lastname: Taheri
authors.affiliation: Department of Immunotherapy and Leishmania Vaccine Research, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Negar
authors.initials: N
authors.lastname: Seyed
authors.affiliation: Department of Immunotherapy and Leishmania Vaccine Research, Pasteur Institute of Iran, Tehran, Iran. s_rafati@yahoo.com.
authors.forename: Sima
authors.initials: S
authors.lastname: Rafati
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004353:Drug Evaluation, Preclinical; D018503:Genome, Protozoan / Q000235:genetics*; D005838:Genotype; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007891:Leishmania / Q000235:genetics* / Q000276:immunology*; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D061848:Optical Imaging; D016052:Protozoan Vaccines / Q000276:immunology*; D014162:Transfection / Q000379:methods*
chemical_list: D016052:Protozoan Vaccines; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D008156:Luciferases
keywords: Bioluminescence; Drug screening; In vivo imaging; Leishmania transfection; Reporter genes; Vaccine