title: Hematobiochemical changes in ehrlichiosis in dogs of Anand region, Gujarat.
abstract: OBJECTIVE
  The present research work was undertaken to study the diagnostic importance of hematobiochemical changes in naturally occurring ehrlichiosis in dogs of Anand region, Gujarat irrespective of their age, breed, and sex.
  
  
  METHODS
  Blood samples from a total of 29 dogs of Anand region of Gujarat state were screened for detection of anti-Ehrlichia canis antibodies using Immunocomb(®) rapid diagnostic kit (Biogal Galed Laboratories, Israel) and subjected to estimation of hematobiochemical parameters by auto hematology analyzers at College of Veterinary Science and Animal Husbandry, Anand. Statistical analysis, interpretation and comparison of hematobiochemical changes with scientific literature was carried out in order to understand the pathophysiology of the disease.
  
  
  RESULTS
  Of 29 dogs, 18 were positive for naturally occurring ehrlichiosis based on the presence of anti-E. canis antibodies while 11 were negative. Haematology evinced that the mean values of hemoglobin, total erythrocyte counts, platelet count and packed cell volume in dogs with ehrlichiosis decreased significantly (p<0.01) in comparison to healthy dogs. Among differential leucocyte count, mean values of lymphocytes decreased, neutrophils increased, eosinophils decreased and basophils decreased significantly (p<0.05) in dogs with ehrlichiosis in comparison to healthy dogs while statistically non-significant (p>0.05) difference was observed in values of monocytes in dogs with ehrlichiosis and healthy dogs. Among various red blood cells indices, the mean values of mean corpuscular hemoglobin concentration increased significantly (p<0.01) in dogs with ehrlichiosis in comparison to healthy dogs. Serum biochemistry revealed significant (p<0.01) increase in serum glutamic pyruvic transaminase, serum glutamic oxaloacetic transaminase and creatinine levels as well as decrease in total protein levels in dogs with ehrlichiosis as compared to healthy dogs.
  
  
  CONCLUSIONS
  Clinical importance of hematobiochemical changes in 18 natural cases of ehrlichiosis in dogs of Anand region, Gujarat irrespective of their age, breed and sex is discussed, which would aid new insights in diagnosis and therapeutic management.
authors.affiliation: Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India.
authors.forename: C M
authors.initials: CM
authors.lastname: Bhadesiya
authors.affiliation: Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Raval
keywords: anti-Ehrlichia canis antibodies; dogs; ehrlichiosis; hematobiochemical changes