title: Variable effect of maternal oral glucose load on circulating cell-free placental mRNAs.
abstract: BACKGROUND
  It is not known whether fasting affects levels of circulating placenta-specific transcripts.
  
  
  OBJECTIVE
  To assess whether a glucose load affects circulating placenta-specific transcripts.
  
  
  METHODS
  RNA was extracted from paired blood samples (fasting and 1-h post 75 g oral glucose) from 22 women. Placenta-specific genes were measured by RT-qPCR.
  
  
  RESULTS
  There was no change in ADM, CSH1, PAPPA2, PSG1 or TAC3 expression between fasting and post-glucose states. However, HTRA1 decreased after glucose load.
  
  
  CONCLUSIONS
  Maternal fasting state does not influence expression of the majority of placenta-specific genes but may need to be accounted for when validating biomarkers of placental disease.
authors.affiliation: a Translational Obstetrics Group, Mercy Hospital for Women , Melbourne , Heidelberg , Australia.|b Department of Obstetrics and Gynaecology , The University of Melbourne , Melbourne , Australia , and.|c Perinatal Department, Mercy Hospital for Women , Melbourne , Heidelberg , Australia.
authors.forename: Teresa Mary
authors.initials: TM
authors.lastname: MacDonald
authors.affiliation: a Translational Obstetrics Group, Mercy Hospital for Women , Melbourne , Heidelberg , Australia.|b Department of Obstetrics and Gynaecology , The University of Melbourne , Melbourne , Australia , and.
authors.forename: Tu'uhevaha Joy
authors.initials: TJ
authors.lastname: Kaitu'u-Lino
authors.affiliation: b Department of Obstetrics and Gynaecology , The University of Melbourne , Melbourne , Australia , and.|c Perinatal Department, Mercy Hospital for Women , Melbourne , Heidelberg , Australia.
authors.forename: Susan Philippa
authors.initials: SP
authors.lastname: Walker
authors.affiliation: c Perinatal Department, Mercy Hospital for Women , Melbourne , Heidelberg , Australia.
authors.forename: Kirsten Margaret
authors.initials: KM
authors.lastname: Dane
authors.affiliation: b Department of Obstetrics and Gynaecology , The University of Melbourne , Melbourne , Australia , and.|c Perinatal Department, Mercy Hospital for Women , Melbourne , Heidelberg , Australia.
authors.forename: Elizabeth Beatrice
authors.initials: EB
authors.lastname: Lockie
authors.affiliation: a Translational Obstetrics Group, Mercy Hospital for Women , Melbourne , Heidelberg , Australia.|b Department of Obstetrics and Gynaecology , The University of Melbourne , Melbourne , Australia , and.
authors.forename: Stephen
authors.initials: S
authors.lastname: Tong
authors.affiliation: a Translational Obstetrics Group, Mercy Hospital for Women , Melbourne , Heidelberg , Australia.
authors.forename: Clare Louise
authors.initials: CL
authors.lastname: Whitehead
authors.affiliation: a Translational Obstetrics Group, Mercy Hospital for Women , Melbourne , Heidelberg , Australia.|b Department of Obstetrics and Gynaecology , The University of Melbourne , Melbourne , Australia , and.|c Perinatal Department, Mercy Hospital for Women , Melbourne , Heidelberg , Australia.
authors.forename: Lisa
authors.initials: L
authors.lastname: Hui
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D005215:Fasting / Q000378:metabolism*; D005260:Female; D005317:Fetal Growth Retardation / Q000097:blood; D015870:Gene Expression; D005947:Glucose / Q000378:metabolism*; D000074289:High-Temperature Requirement A Serine Peptidase 1; D006801:Humans; D010920:Placenta / Q000378:metabolism*; D021041:Placental Circulation; D011247:Pregnancy; D011266:Pregnancy-Associated Plasma Protein-A / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000097:blood*; D012697:Serine Endopeptidases / Q000097:blood* / Q000235:genetics; D055815:Young Adult
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; D000074289:High-Temperature Requirement A Serine Peptidase 1; C474012:HTRA1 protein, human; D012697:Serine Endopeptidases; D011266:Pregnancy-Associated Plasma Protein-A; D005947:Glucose
keywords: Biomarker; fasting; gene; pregnancy; transcription