title: High Frequency of Chlamydia trachomatis Mixed Infections Detected by Microarray Assay in South American Samples.
abstract: Chlamydia trachomatis is one of the most common sexually transmitted infections worldwide. Based on sequence variation in the ompA gene encoding the major outer membrane protein, the genotyping scheme distinguishes 17 recognized genotypes, i.e. A, B, Ba, C, D, Da, E, F, G, H, I, Ia, J, K, L1, L2, and L3. Genotyping is an important tool for epidemiological tracking of C. trachomatis infections, including the revelation of transmission pathways and association with tissue tropism and pathogenicity. Moreover, genotyping can be useful for clinicians to establish the correct treatment when LGV strains are detected. Recently a microarray assay was described that offers several advantages, such as rapidity, ease of standardization and detection of mixed infections. The aim of this study was to evaluate the performance of the DNA microarray-based assay for C. trachomatis genotyping of clinical samples already typed by PCR-RFLP from South America. The agreement between both typing techniques was 90.05% and the overall genotype distribution obtained with both techniques was similar. Detection of mixed-genotype infections was significantly higher using the microarray assay (8.4% of cases) compared to PCR-RFLP (0.5%). Among 178 samples, the microarray assay identified 10 ompA genotypes, i.e. D, Da, E, F, G, H, I, J, K and L2. The most predominant type was genotype E, followed by D and F.
authors.affiliation: Universidad de Buenos Aires. Cátedra de Microbiología Clínica, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
authors.forename: Lucía
authors.initials: L
authors.lastname: Gallo Vaulet
authors.affiliation: Universidad de Buenos Aires. Cátedra de Microbiología Clínica, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
authors.forename: Carolina
authors.initials: C
authors.lastname: Entrocassi
authors.affiliation: Universidad de Buenos Aires. Cátedra de Microbiología Clínica, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
authors.forename: Ana I
authors.initials: AI
authors.lastname: Portu
authors.affiliation: Facultad de Medicina, Universidad de San Sebastián, Concepción, Chile.
authors.forename: Erica
authors.initials: E
authors.lastname: Castro
authors.affiliation: Sección Bioquímica Microbiológica, Servicio de Bioquímica, Hospital Nacional Profesor A. Posadas, Buenos Aires, Argentina.
authors.forename: Susana
authors.initials: S
authors.lastname: Di Bartolomeo
authors.affiliation: Institute of Molecular Pathogenesis, Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Jena, Germany.
authors.forename: Anke
authors.initials: A
authors.lastname: Ruettger
authors.affiliation: Institute of Molecular Pathogenesis, Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Jena, Germany.
authors.forename: Konrad
authors.initials: K
authors.lastname: Sachse
authors.affiliation: Universidad de Buenos Aires. Cátedra de Microbiología Clínica, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Rodriguez Fermepin
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000453:epidemiology* / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000235:genetics* / Q000302:isolation & purification; D060085:Coinfection; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D052776:Female Urogenital Diseases / Q000453:epidemiology / Q000382:microbiology; D005838:Genotype; D006801:Humans; D008297:Male; D052801:Male Urogenital Diseases / Q000453:epidemiology / Q000382:microbiology; D025202:Molecular Diagnostic Techniques / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D013020:South America / Q000453:epidemiology
chemical_list: D004269:DNA, Bacterial