title: Development of functional trait biomarkers for trace metal exposure in freshwater clams (Musculium spp.).
abstract: Exposure to trace metals typically causes oxidative stress; these consequences are better-characterized in estuarine and marine species than in freshwater species. How cellular-level responses to metal pollution influence whole-organism and population-level traits is poorly understood. We tested whether exposure to single metals (zinc and cadmium) and to metal mixtures (water in equilibrium with sediment from a highly polluted lake) alters two ecologically-relevant traits in freshwater clams, locomotion and reproduction. Fingernail clams (Musculium spp.) from unimpacted habitats were exposed to single metals and the metal mixture for up to 49days. The single metal doses (≤5mg/L Zn and ≤20μg/L Cd) were not toxicologically meaningful as clam survival, burial, and climbing activity did not differ across treatments. Water in equilibrium with the lake sediment contained cadmium, copper, lead, and zinc. Clams exposed to this metal mixture had decreased climbing activity but no change in burial activity. Metal-exposed clams had lower fecundity (number of shelled juveniles extruded by adult clams) and patterns in metal accumulation corresponded with lake sediment dose and clam activity. In contrast to the functional traits, stress protein expression and whole-clam glycogen content did not vary across treatment groups. These results indicate that fingernail clams of the genus Musculium are appropriate for development as sentinel species for metal pollution and can serve as a model for determining how metal pollution alters metabolic allocation patterns in freshwater organisms.
authors.affiliation: Department of Biology, Eastern Washington University, 258 Science Building, Cheney, WA 99004, USA.
authors.forename: Cody M
authors.initials: CM
authors.lastname: Schoonover
authors.affiliation: Department of Biology, Eastern Washington University, 258 Science Building, Cheney, WA 99004, USA.
authors.forename: Jessica
authors.initials: J
authors.lastname: Wieker
authors.affiliation: Department of Biology, Eastern Washington University, 258 Science Building, Cheney, WA 99004, USA.
authors.forename: Rachelle
authors.initials: R
authors.lastname: Pope
authors.affiliation: Department of Biology, Eastern Washington University, 258 Science Building, Cheney, WA 99004, USA.
authors.forename: Chelsea
authors.initials: C
authors.lastname: Brown
authors.affiliation: Department of Biology, Eastern Washington University, 258 Science Building, Cheney, WA 99004, USA.
authors.forename: Emily
authors.initials: E
authors.lastname: Cooper
authors.affiliation: Department of Biology, Eastern Washington University, 258 Science Building, Cheney, WA 99004, USA.
authors.forename: Jariel
authors.initials: J
authors.lastname: DeWitt
authors.affiliation: Department of Biology, Eastern Washington University, 258 Science Building, Cheney, WA 99004, USA.
authors.forename: Samuel
authors.initials: S
authors.lastname: Gunselman
authors.affiliation: Department of Biology, Eastern Washington University, 258 Science Building, Cheney, WA 99004, USA.
authors.forename: Cory
authors.initials: C
authors.lastname: Jensen
authors.affiliation: Department of Biology, Eastern Washington University, 258 Science Building, Cheney, WA 99004, USA.
authors.forename: Whitney
authors.initials: W
authors.lastname: Stevens
authors.affiliation: Department of Biology, Eastern Washington University, 258 Science Building, Cheney, WA 99004, USA.
authors.forename: Jenae
authors.initials: J
authors.lastname: Yri
authors.affiliation: Department of Geology, Eastern Washington University, 132 Science Building, Cheney, WA 99004, USA.
authors.forename: Carmen
authors.initials: C
authors.lastname: Nezat
authors.affiliation: Department of Biology, Eastern Washington University, 258 Science Building, Cheney, WA 99004, USA. Electronic address: jmatos@ewu.edu.
authors.forename: Joanna
authors.initials: J
authors.lastname: Joyner-Matos
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D049872:Bivalvia / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D002104:Cadmium / Q000378:metabolism / Q000633:toxicity; D018834:Chaperonin 60 / Q000378:metabolism; D003300:Copper / Q000378:metabolism / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D005618:Fresh Water / Q000737:chemistry; D005843:Geography; D019015:Geologic Sediments / Q000737:chemistry*; D006003:Glycogen / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D053208:Kaplan-Meier Estimate; D007814:Larva / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D007854:Lead / Q000378:metabolism / Q000633:toxicity; D008124:Locomotion / Q000187:drug effects; D008670:Metals / Q000378:metabolism / Q000633:toxicity*; D009043:Motor Activity / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D025801:Ubiquitin / Q000378:metabolism; D014861:Washington; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015032:Zinc / Q000378:metabolism / Q000633:toxicity
chemical_list: D015415:Biomarkers; D018834:Chaperonin 60; D018840:HSP70 Heat-Shock Proteins; D008670:Metals; D025801:Ubiquitin; D014874:Water Pollutants, Chemical; D002104:Cadmium; D007854:Lead; D003300:Copper; D006003:Glycogen; D013482:Superoxide Dismutase; D015032:Zinc
keywords: Biomarker; Bivalve; Locomotion; Metal; Pollution; Reproduction