title: Metabolomics analysis reveals 6-benzylaminopurine as a stimulator for improving lipid and DHA accumulation of Aurantiochytriumsp.
abstract: BACKGROUND
  Phytohormones are chemical messengers that have a positive effect on biodiesel production of microalgae at low concentrations. However, the effect of phytohormone 6-benzylaminopurine on lipid and docosahexaenoic acid (DHA) production in marine DHA-producer Aurantiochytrium has never been reported. In this study, a GC-MS-based metabolomics method combined with a multivariate analysis is applied to reveal the metabolic mechanism of 6-benzylaminopurine enhancing production of lipid and DHA in Aurantiochytrium sp.YLH70.
  
  
  RESULTS
  In total, 71 metabolites were identified by GC-MS. The PCA model revealed that 76.9% of metabolite variation was related to 6-benzylaminopurine treatment, and overall metabolomics profiles between the 6-benzylaminopurine and control groups were clearly discriminated. Forty-six metabolites identified by the PLS-DA model were responsible for responding to 6-benzylaminopurine. Metabolic analysis showed that 6-benzylaminopurine could accelerate the rate of utilization of glucose in Aurantiochytrium sp. YLH70, and the metabolic flux from glycolysis, TCA cycle and mevalonate pathway to fatty acids biosynthesis was promoted. Moreover, the anti-stress mechanism in Aurantiochytrium sp.YLH70 might be induced by 6-benzylaminopurine.
  
  
  CONCLUSIONS
  Metabolomics is a suitable tool to discover the metabolic mechanism for improving lipid and DHA accumulation in a microorganism. 6-benzylaminopurine has the potential to stimulate lipid and DHA production of Aurantiochytrium sp.YLH70 for industrial purposes. © 2015 The Authors.Journal of Chemical Technology & Biotechnologypublished by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
authors.affiliation: College of Biological and Environmental Engineering Zhejiang University of Technology No.18, Chaowang Road Hangzhou 310014 People's Republic of China.
authors.forename: Xin-Jun
authors.initials: XJ
authors.lastname: Yu
authors.affiliation: College of Biological and Environmental Engineering Zhejiang University of Technology No.18, Chaowang Road Hangzhou 310014 People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.affiliation: College of Biological and Environmental Engineering Zhejiang University of Technology No.18, Chaowang Road Hangzhou 310014 People's Republic of China.
authors.forename: Jian-Yong
authors.initials: JY
authors.lastname: Zheng
authors.affiliation: College of Biological and Environmental Engineering Zhejiang University of Technology No.18, Chaowang Road Hangzhou 310014 People's Republic of China.
authors.forename: Ya-Qi
authors.initials: YQ
authors.lastname: Sun
authors.affiliation: College of Biological and Environmental Engineering Zhejiang University of Technology No.18, Chaowang Road Hangzhou 310014 People's Republic of China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Wang
keywords: 6‐benzylaminopurine; Aurantiochytrium sp; GC‐MS; TCA cycle; docosahexaenoic acid (DHA); metabolomics