title: EX VIVO ADHERENCE TO MURINE ILEAL, BIOFILM FORMATION ABILITY AND PRESENCE OF ADHERENCE-ASSOCIATED OF HUMAN AND ANIMAL DIARRHEAGENIC ESCHERICHIA COLI.
abstract: Diarrheagenic Escherichia coli (DEC) are important bacteria causing gastrointestinal infection, which can lead to severe forms of illnesses. This study focused on DEC adherent capabilities using murine intestinal tissue as a model. Ex vivo adherence results showed that enteroaggregative E. coli (EAEC) strain PSU280 exhibited the highest level of adherence, followed by strains from ETEC category. Scanning electron micrographs displayed tight binding and putative bacterial curli fibers, including putative fimbrial structures. The presence of putative curli fibers was confirmed by the presence of csgA, a curli major structural subunit gene. Five and 3 of 15 DEC possessed lpf (encoding long polar fimbriae) and agn43 (encoding antigen43), respectively. Comparable biofilm formation efficiency but variable levels autoaggregation were observed among the DEC strains. In addition, yeast agglutination could be visualized in 11/15 strains. This study demonstrates the adherent ability of DEC strains isolated in southern Thailand as well as a number of crucial adherence-associated genes, information of importance to the understanding of DEC pathogenesis in this region of the country.
authors.forename: Kannika
authors.initials: K
authors.lastname: Sukkua
authors.forename: Pattamarat
authors.initials: P
authors.lastname: Rattanachuay
authors.forename: Pharanai
authors.initials: P
authors.lastname: Sukhumungoon
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D018441:Biofilms; D003967:Diarrhea / Q000382:microbiology*; D004926:Escherichia coli / Q000502:physiology*; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000502:physiology*; D006801:Humans; D051379:Mice
chemical_list: D029968:Escherichia coli Proteins