title: SPR-based plastic optical fibre biosensor for the detection of C-reactive protein in serum.
abstract: A plastic optical fibre biosensor based on surface plasmon resonance for the detection of C-reactive protein (CRP) in serum is proposed. The biosensor was integrated into a home-made thermo-stabilized microfluidic system that allows avoiding any thermal and/or mechanical fluctuation and maintaining the best stable conditions during the measurements. A working range of 0.006-70 mg L-1and a limit of detection of 0.009 mg L-1were achieved. These results are among the best compared to other SPR-based biosensors for CRP detection, especially considering that they were achieved in a real and complex medium, i.e. serum. In addition, since the sensor performances satisfy those requested in physiologically-relevant clinical applications, the whole biosensing platform could well address high sensitive, easy to realize, real-time, label-free, portable and low cost diagnosis of CRP for future lab-on-a-chip applications. 3D sketch (left) of the thermo-stabilized home-made flow cell developed to house the SPR-based plastic optical fibre biosensor. Exemplary response curve (shift of the SPR wavelength versus time) of the proposed biosensor (right) for the detection of C-reactive protein in serum.
authors.affiliation: Quantum Optics Group, Department of Physics, University of Isfahan, Hezar Jarib street, 98311, Isfahan, Islamic Republic of Iran.
authors.forename: Ayda
authors.initials: A
authors.lastname: Aray
authors.affiliation: Institute of Applied Physics "Nello Carrara", National Research Council, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Chiavaioli
authors.affiliation: Quantum Optics Group, Department of Physics, University of Isfahan, Hezar Jarib street, 98311, Isfahan, Islamic Republic of Iran.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Arjmand
authors.affiliation: Institute of Applied Physics "Nello Carrara", National Research Council, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Italy. c.trono@ifac.cnr.it.
authors.forename: Cosimo
authors.initials: C
authors.lastname: Trono
authors.affiliation: Institute of Applied Physics "Nello Carrara", National Research Council, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Tombelli
authors.affiliation: Institute of Applied Physics "Nello Carrara", National Research Council, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Italy.
authors.forename: Ambra
authors.initials: A
authors.lastname: Giannetti
authors.affiliation: Department of Industrial and Information Engineering, Second University of Naples, Via Roma 29, 81031, Aversa, Italy.
authors.forename: Nunzio
authors.initials: N
authors.lastname: Cennamo
authors.affiliation: Quantum Optics Group, Department of Physics, University of Isfahan, Hezar Jarib street, 98311, Isfahan, Islamic Republic of Iran.
authors.forename: Mahmood
authors.initials: M
authors.lastname: Soltanolkotabi
authors.affiliation: Department of Industrial and Information Engineering, Second University of Naples, Via Roma 29, 81031, Aversa, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Zeni
authors.affiliation: Institute of Applied Physics "Nello Carrara", National Research Council, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Baldini
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002097:C-Reactive Protein / Q000032:analysis*; D006801:Humans; D056656:Lab-On-A-Chip Devices; D055100:Optical Fibers; D010969:Plastics; D044967:Serum / Q000737:chemistry; D020349:Surface Plasmon Resonance
chemical_list: D010969:Plastics; D002097:C-Reactive Protein
keywords: C-reactive protein; microfluidic system; plastic optical fibre; serum; surface plasmon resonance