title: Cell cycle status in AML blast cells from peripheral blood, bone marrow aspirates and trephines and implications for biological studies and treatment.
abstract: Using immunohistochemistry and flow cytometry to define phases of the cell cycle, this study shows that a high proportion of acute myeloid leukaemia (AML) blasts obtained from trephine biopsies are cycling, whereas >95% of peripheral blood-derived blasts are arrested in G1 . Results obtained from bone marrow aspirates are more similar to those from blood rather than from trephine biopsies. These differences were confirmed by gene expression profiling in a patient with high count AML. This has implications for cell cycle and other biological studies using aspirates rather than trephine biopsies and for the use of cell mobilising agents before chemotherapy.
authors.affiliation: Department of Haematology, University College London Cancer Institute, London, UK.
authors.forename: Rob S
authors.initials: RS
authors.lastname: Sellar
authors.affiliation: Department of Haematology, University College London Cancer Institute, London, UK.
authors.forename: Laura
authors.initials: L
authors.lastname: Fraser
authors.affiliation: Department of Haematology, University College London Cancer Institute, London, UK.
authors.forename: Asim
authors.initials: A
authors.lastname: Khwaja
authors.affiliation: Department of Haematology, University College London Cancer Institute, London, UK.
authors.forename: Rosemary E
authors.initials: RE
authors.lastname: Gale
authors.affiliation: Department of Histopathology, University College London Hospitals, London, UK.|Department of Cellular Pathology, University College London Hospitals, London, UK.
authors.forename: Teresa
authors.initials: T
authors.lastname: Marafioti
authors.affiliation: Department of Histopathology, University College London Hospitals, London, UK.|Department of Cellular Pathology, University College London Hospitals, London, UK.
authors.forename: Ayse
authors.initials: A
authors.lastname: Akarca
authors.affiliation: Genetics and Genomic Medicine Programme, UCL Institute of Child Health, London, UK.
authors.forename: Mike
authors.initials: M
authors.lastname: Hubank
authors.affiliation: Genetics and Genomic Medicine Programme, UCL Institute of Child Health, London, UK.
authors.forename: Tony
authors.initials: T
authors.lastname: Brooks
authors.affiliation: Department of Histopathology, University College London Hospitals, London, UK.
authors.forename: Kai
authors.initials: K
authors.lastname: Stoeber
authors.affiliation: Department of Histopathology, University College London Hospitals, London, UK.
authors.forename: Gareth
authors.initials: G
authors.lastname: Williams
authors.affiliation: Department of Haematology, University College London Cancer Institute, London, UK.
authors.forename: David C
authors.initials: DC
authors.lastname: Linch
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy; D001752:Blast Crisis / Q000473:pathology*; D001854:Bone Marrow Cells / Q000473:pathology; D002453:Cell Cycle; D059447:Cell Cycle Checkpoints; D005260:Female; D016193:G1 Phase; D020869:Gene Expression Profiling; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009360:Neoplastic Cells, Circulating / Q000473:pathology; D014207:Trephining
keywords: AML; cell cycle; flow cytometry; gene expression; immunocytochemistry