title: Short-chain fatty acids production and microbial community in sludge alkaline fermentation: Long-term effect of temperature.
abstract: Sludge alkaline fermentation has been reported to achieve efficient short-chain fatty acids (SCFAs) production. Temperature played important role in further improved SCFAs production. Long-term SCFAs production from sludge alkaline fermentation was compared between mesotherm (30±2°C) and microtherm (15±2°C). The study of 90days showed that mesotherm led to 2.2-folds production of SCFAs as microtherm and enhanced the production of acetic acid as major component of SCFAs. Soluble protein and carbohydrate at mesotherm was 2.63-folds as that at microtherm due to higher activities of protease and α-glucosidase, guaranteeing efficient substrates to produce SCFAs. Illumina MiSeq sequencing revealed that microtherm increased the abundance of Corynebacterium, Alkaliflexus, Pseudomonas and Guggenheimella, capable of enhancing hydrolysis. Hydrolytic bacteria, i.e. Alcaligenes, Anaerolinea and Ottowia, were enriched at mesotherm. Meanwhile, acidogenic bacteria showed higher abundance at mesotherm than microtherm. Therefore, enrichment of functional bacteria and higher microbial activities resulted in the improved SCFAs at mesotherm.
authors.affiliation: Engineering Research Center of Beijing, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Engineering Research Center of Beijing, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Engineering Research Center of Beijing, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, PR China; Department of Civil and Environmental Engineering, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: Baikun
authors.initials: B
authors.lastname: Li
authors.affiliation: Engineering Research Center of Beijing, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: Engineering Research Center of Beijing, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Shuying
authors.initials: S
authors.lastname: Wang
authors.affiliation: Engineering Research Center of Beijing, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, PR China. Electronic address: pyz@bjut.edu.cn.
authors.forename: Yongzhen
authors.initials: Y
authors.lastname: Peng
mesh_terms: D000468:Alkalies / Q000494:pharmacology*; D001419:Bacteria / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis; D044822:Biodiversity; D019149:Bioreactors / Q000382:microbiology; D002241:Carbohydrates / Q000032:analysis; D005227:Fatty Acids / Q000378:metabolism; D005232:Fatty Acids, Volatile / Q000096:biosynthesis*; D005285:Fermentation / Q000187:drug effects; D006868:Hydrolysis; D010802:Phylogeny; D012722:Sewage / Q000382:microbiology*; D012995:Solubility; D013696:Temperature; D014866:Waste Products; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D000468:Alkalies; D001426:Bacterial Proteins; D002241:Carbohydrates; D005227:Fatty Acids; D005232:Fatty Acids, Volatile; D012722:Sewage; D014866:Waste Products; D000520:alpha-Glucosidases
keywords: Alkaline fermentation; Enzyme activity; Mesotherm and microtherm; Microbial community; Short-chain fatty acids (SCFAs); Waste activated sludge (WAS)