title: Heavy metals removal from wastewater using extracellular polymeric substances produced by Cloacibacterium normanense in wastewater sludge supplemented with crude glycerol and study of extracellular polymeric substances extraction by different methods.
abstract: Extracellular polymeric substances synthesis by Cloacibacterium was affected by different concentrations of glycerol in the medium. The concentration of EPS in 72h fermentation was increased from 13g/L with no external carbon supplementation to 21.3±0.7g/L with 2% (w/v) crude glycerol addition. Physical and chemical extraction methods (heating, centrifugation and ethylene diamine tetra-acetic acid (EDTA)) were used in this study and their performance to extract EPS was compared. A significant variation in concentration of extracted B-EPS (broth-EPS) by heating (20.8±0.5g/L) and centrifugation (21.3±0.7g/L) extraction methods was not observed. However, in case of extraction with EDTA (5g/L), the B-EPS concentration extracted was 25.5±0.9g/L, which exhibited high flocculation activity of 95.3±0.5% at optimum dose of 23.1mgB-EPS/gkaolin. Moreover, Ni removal efficiency of 80% from primary treated wastewater was achieved using 35mg/L of B-EPS extracted by centrifugation method.
authors.affiliation: Université du Québec, Institut national de la recherche scientifique, Centre Eau, Terre & Environnement, 490 de la Couronne, Québec G1K 9A9, Canada.
authors.forename: Klai
authors.initials: K
authors.lastname: Nouha
authors.affiliation: Université du Québec, Institut national de la recherche scientifique, Centre Eau, Terre & Environnement, 490 de la Couronne, Québec G1K 9A9, Canada.
authors.forename: Ram Saurabh
authors.initials: RS
authors.lastname: Kumar
authors.affiliation: Université du Québec, Institut national de la recherche scientifique, Centre Eau, Terre & Environnement, 490 de la Couronne, Québec G1K 9A9, Canada. Electronic address: tyagi@ete.inrs.ca.
authors.forename: R D
authors.initials: RD
authors.lastname: Tyagi
mesh_terms: D041963:Bacteroidetes / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D001704:Biopolymers / Q000737:chemistry*; D002241:Carbohydrates / Q000032:analysis; D002244:Carbon / Q000494:pharmacology; D002498:Centrifugation; D005285:Fermentation / Q000187:drug effects; D005427:Flocculation; D005990:Glycerol / Q000494:pharmacology*; D007616:Kaolin / Q000737:chemistry; D019216:Metals, Heavy / Q000302:isolation & purification*; D011506:Proteins / Q000032:analysis; D012722:Sewage / Q000382:microbiology*; D062065:Wastewater / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*; D018508:Water Purification / Q000379:methods*
chemical_list: D001704:Biopolymers; D002241:Carbohydrates; D002244:Carbon; D005990:Glycerol; D007616:Kaolin; D019216:Metals, Heavy; D011506:Proteins; D012722:Sewage; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Crude glycerol; Extracellular polymeric substance; Flocculation activity; Heavy metal removal; Wastewater sludge