title: Regulatory role of Cdx-2 and Taq I polymorphism of vitamin D receptor gene on chemokine expression in pulmonary tuberculosis.
abstract: Vitamin D receptor (VDR) gene variants have been shown to be regulating the immune response in tuberculosis. We studied the regulatory role of VDR promoter Cdx-2 and 3'UTR TaqI gene variants on chemokine levels from culture filtrate antigen (CFA) stimulated with or without 1,25(OH)2D3 treated peripheral blood mononuclear cells of 50 pulmonary tuberculosis patients (PTB) and 51 normal healthy controls (HCs). In CFA with 1,25(OH)2D3 treated cultures, the MIP-1α, MIP-1β, RANTES levels were significantly decreased in Cdx-2 AA genotype compared to GG genotype, while a significantly increased MIG level was observed in Cdx-2 AA genotype (p<0.05). In TaqI polymorphism, tt genotype significantly decreased MIP-1β and RANTES levels compared to TT genotype. Moreover, a significantly increased level of IP-10 and MIG was observed in TaqI tt genotype compared with TT genotype (p<0.05). The results suggests that the 1,25(OH)2D3 may alter the chemokine response through the VDR polymorphic variants during infection.
authors.affiliation: Department of Immunology, National Institute for Research in Tuberculosis (Formerly Tuberculosis Research Centre), Indian Council of Medical Research, 1, Mayor Sathyamoorthy Road, Chennai 600 031, India. Electronic address: haritech2001@yahoo.com.
authors.forename: M
authors.initials: M
authors.lastname: Harishankar
authors.affiliation: Department of Immunology, National Institute for Research in Tuberculosis (Formerly Tuberculosis Research Centre), Indian Council of Medical Research, 1, Mayor Sathyamoorthy Road, Chennai 600 031, India.
authors.forename: P
authors.initials: P
authors.lastname: Selvaraj
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics / Q000276:immunology; D000328:Adult; D000071616:CDX2 Transcription Factor / Q000235:genetics*; D002478:Cells, Cultured; D018925:Chemokines / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D005838:Genotype; D006801:Humans; D007109:Immunity / Q000235:genetics; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000382:microbiology; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics; D018167:Receptors, Calcitriol / Q000235:genetics*; D019914:Taq Polymerase; D014397:Tuberculosis, Pulmonary / Q000276:immunology*; D055815:Young Adult
chemical_list: D020413:3' Untranslated Regions; D000071616:CDX2 Transcription Factor; D018925:Chemokines; D018167:Receptors, Calcitriol; D019914:Taq Polymerase
keywords: Chemokine; Culture filtrate antigen and tuberculosis; Gene polymorphism; Vitamin D receptor