title: Inhibition of Intestinal α-Glucosidase and Glucose Absorption by Feruloylated Arabinoxylan Mono- and Oligosaccharides from Corn Bran and Wheat Aleurone.
abstract: The effect of feruloylated arabinoxylan mono- and oligosaccharides (FAXmo) on mammalian α-glucosidase and glucose transporters was investigated using human Caco-2 cells, rat intestinal acetone powder, and Xenopus laevis oocytes. The isolated FAXmo from wheat aleurone and corn bran were identified to have degree of polymerization (DP) of 4 and 1, respectively, by HPLC-MS. Both FAXmo extracts were effective inhibitors of sucrase and maltase functions of the α-glucosidase. The IC50 for FAXmo extracts on Caco-2 cells and rat intestinal α-glucosidase was 1.03-1.65 mg/mL and 2.6-6.5 mg/mL, respectively. Similarly, glucose uptake in Caco-2 cells was inhibited up to 40%. The inhibitory effect of FAXmo was dependent on their ferulic acid (FA) content (R = 0.95). Sodium independent glucose transporter 2 (GLUT2) activity was completely inhibited by FAXmo in oocytes injected to express GLUT2. Our results suggest that ferulic acid and feruloylated arabinoxylan mono-/oligosaccharides have potential for use in diabetes management.
authors.affiliation: Department of Food Science, University of Manitoba, Winnipeg, MB, Canada R3T 2N2.
authors.forename: Lovemore Nkhata
authors.initials: LN
authors.lastname: Malunga
authors.affiliation: Department of Human Nutrition, University of Manitoba, Winnipeg, MB, Canada R3T 2N2.
authors.forename: Peter
authors.initials: P
authors.lastname: Eck
authors.affiliation: Department of Food Science, University of Manitoba, Winnipeg, MB, Canada R3T 2N2; Richardson Centre for Functional Foods & Nutraceuticals, University of Manitoba, Winnipeg, MB, Canada R3T 2N2.
authors.forename: Trust
authors.initials: T
authors.lastname: Beta