title: Comparison of Luminex xTAG® RVP fast assay and real time RT-PCR for the detection of respiratory viruses in adults with community-acquired pneumonia.
abstract: Community-acquired pneumonia (CAP) is the third cause of death worldwide. Viruses are frequently detected in adult CAP. Highly sensitive diagnostic techniques should be used due to poor viral shedding. Different sampling methods can affect viral detection, being necessary to establish the optimal type of sample for identifying respiratory viruses in adults. The detection rates of respiratory viruses by Luminex xTAG® RVP fast assay, real time RT-PCR (rtRT-PCR) (Sacace®), and immunofluorescence assay (IFA) in adult CAP were performed in nasopharyngeal swabs (NPS) and aspirates (NPA) from 179 hospitalized adults. Positivity was 47.5% for Luminex®, 42.5% for rtRT-PCR (P = 0.3), and 2.7% for IFA (2.7%) (P < 0.0). The sensitivity, specificity, and kappa coefficient of xTAG® RVP compared with rtRT-PCR were 84.2%, 79.6%, and 0.62%, respectively. Luminex® and rtRT-PCR detected 65 (58.0%) and 57 (50.9%) viruses in 112 NPA and 35 (34.3%) and 31 (30.4%) in 102 NPS, respectively (P < 0.01). xTAG® RVP is appropriate for detecting respiratory viruses in CAP adults. Both molecular techniques yielded better results with nasopharyngeal aspirate than swabs.
authors.affiliation: Program of Virology, Faculty of Medicine, University of Chile, Santiago, Chile.
authors.forename: Vivian
authors.initials: V
authors.lastname: Luchsinger
authors.affiliation: Program of Virology, Faculty of Medicine, University of Chile, Santiago, Chile.
authors.forename: Yara
authors.initials: Y
authors.lastname: Prades
authors.affiliation: Clinical Hospital of the University of Chile, Santiago, Chile.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Ruiz
authors.affiliation: Dr. Lucio Cordova Hospital of Infectious Diseases, Santiago, Chile.
authors.forename: Rolando
authors.initials: R
authors.lastname: Pizarro
authors.affiliation: San José Hospital Complex, Santiago, Chile.
authors.forename: Patricio
authors.initials: P
authors.lastname: Rossi
authors.affiliation: Program of Virology, Faculty of Medicine, University of Chile, Santiago, Chile.
authors.forename: Luis
authors.initials: L
authors.lastname: Lizama
authors.affiliation: Institute of Nutrition and Food Technology, University of Chile, Santiago, Chile.
authors.forename: María Luisa
authors.initials: ML
authors.lastname: Garmendia
authors.affiliation: San José Hospital Complex, Santiago, Chile.
authors.forename: Angela
authors.initials: A
authors.lastname: Meza
authors.affiliation: Program of Virology, Faculty of Medicine, University of Chile, Santiago, Chile.
authors.forename: Carmen
authors.initials: C
authors.lastname: Larrañaga
authors.affiliation: Program of Virology, Faculty of Medicine, University of Chile, Santiago, Chile.
authors.forename: Luis F
authors.initials: LF
authors.lastname: Avendaño
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017714:Community-Acquired Infections / Q000175:diagnosis* / Q000821:virology*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006760:Hospitalization; D006801:Humans; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods; D009305:Nasopharynx / Q000821:virology; D011024:Pneumonia, Viral / Q000175:diagnosis* / Q000821:virology*; D011933:Reagent Kits, Diagnostic; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014780:Viruses / Q000302:isolation & purification*; D055815:Young Adult
chemical_list: D011933:Reagent Kits, Diagnostic
keywords: Luminex xTAG RVP; community-acquired pneumonia; diagnosis; polymerase chain reaction; respiratory viruses