title: Development of Electrochemiluminescent Serology Assays to Measure the Humoral Response to Antigens of Respiratory Syncytial Virus.
abstract: Sensitive and precise serology assays are needed to measure the humoral response to antigens of respiratory syncytial virus (RSV) following natural infection or vaccination. We developed and evaluated a collection of electrochemiluminescent (ECL) serology assays using four RSV antigens (F, N, Ga and Gb). To assess the merits of ECL technology, the four ECL serology assays were evaluated using a well-characterized "gold standard" panel of acute and convalescent serum samples from fifty-nine RSV-positive and thirty RSV-negative elderly subjects (≥65 years old). The combined results from the four ECL assays demonstrated good concordance to the "gold standard" diagnosis, reaching 95% diagnostic sensitivity and 100% diagnostic specificity. Additionally, a combination of ECL assays provided higher diagnostic sensitivity than a commercially available diagnostic ELISA or cell-based microneutralization assay. In summary, these data demonstrate the advantages of using ECL-based serology assays and highlight their use as a sensitive diagnostic approach to detect recent RSV infection in an elderly population.
authors.affiliation: Applied Immunology and Microbiology, MedImmune, Mountain View, California, United States of America.
authors.forename: Sarah V
authors.initials: SV
authors.lastname: Maifeld
authors.affiliation: Applied Immunology and Microbiology, MedImmune, Mountain View, California, United States of America.
authors.forename: Bodrey
authors.initials: B
authors.lastname: Ro
authors.affiliation: Applied Immunology and Microbiology, MedImmune, Mountain View, California, United States of America.
authors.forename: Hoyin
authors.initials: H
authors.lastname: Mok
authors.affiliation: Applied Immunology and Microbiology, MedImmune, Mountain View, California, United States of America.
authors.forename: Marla
authors.initials: M
authors.lastname: Chu
authors.affiliation: Non-clinical Biostatistics, MedImmune, Gaithersburg, Maryland, United States of America.
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.affiliation: Non-clinical Biostatistics, MedImmune, Gaithersburg, Maryland, United States of America.
authors.forename: Ryan
authors.initials: R
authors.lastname: Yamagata
authors.affiliation: Vaccine and Analytical Sciences, MedImmune, Mountain View, California, United States of America.
authors.forename: Tansy
authors.initials: T
authors.lastname: Leonardson
authors.affiliation: Vaccine and Analytical Sciences, MedImmune, Mountain View, California, United States of America.
authors.forename: Vera
authors.initials: V
authors.lastname: Chio
authors.affiliation: Applied Immunology and Microbiology, MedImmune, Mountain View, California, United States of America.
authors.forename: Bandita
authors.initials: B
authors.lastname: Parhy
authors.affiliation: Vaccine and Analytical Sciences, MedImmune, Mountain View, California, United States of America.
authors.forename: Samuel
authors.initials: S
authors.lastname: Park
authors.affiliation: Purification Process Sciences, MedImmune, Gaithersburg, Maryland, United States of America.
authors.forename: Marcia
authors.initials: M
authors.lastname: Carlson
authors.affiliation: Cell Culture and Fermentation Sciences, MedImmune, Gaithersburg, Maryland, United States of America.
authors.forename: Shushil
authors.initials: S
authors.lastname: Machhi
authors.affiliation: Department of Infectious Disease and Vaccines, Gaithersburg, California, United States of America.
authors.forename: Nancy
authors.initials: N
authors.lastname: Ulbrandt
authors.affiliation: Department of Medicine, University of Rochester School of Medicine and Dentistry, and Department of Medicine, Rochester General Hospital, Rochester, New York, United States of America.
authors.forename: Ann R
authors.initials: AR
authors.lastname: Falsey
authors.affiliation: Department of Medicine, University of Rochester School of Medicine and Dentistry, and Department of Medicine, Rochester General Hospital, Rochester, New York, United States of America.
authors.forename: Edward E
authors.initials: EE
authors.lastname: Walsh
authors.affiliation: Applied Immunology and Microbiology, MedImmune, Mountain View, California, United States of America.
authors.forename: C Kathy
authors.initials: CK
authors.lastname: Wang
authors.affiliation: Translational Medicine, MedImmune, Gaithersburg, Maryland, United States of America.
authors.forename: Mark T
authors.initials: MT
authors.lastname: Esser
authors.affiliation: Applied Immunology and Microbiology, MedImmune, Mountain View, California, United States of America.
authors.forename: Fengrong
authors.initials: F
authors.lastname: Zuo
mesh_terms: D000368:Aged; D061067:Antibodies, Monoclonal, Humanized / Q000276:immunology; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D056724:Immunity, Humoral; D007223:Infant; D008163:Luminescent Measurements / Q000379:methods*; D009500:Neutralization Tests; D019590:Nucleocapsid Proteins / Q000276:immunology; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis* / Q000276:immunology*; D018113:Respiratory Syncytial Virus, Human / Q000276:immunology*; D012698:Serologic Tests / Q000379:methods*; D014759:Viral Envelope Proteins / Q000276:immunology; D014760:Viral Fusion Proteins / Q000276:immunology
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000956:Antigens, Viral; D019590:Nucleocapsid Proteins; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; C116204:attachment protein G; C506968:motavizumab