title: Sero-epidemiological value of some hydatid cyst antigen in diagnosis of human cystic echinococcosis.
abstract: Cystic echinococcosis (CE) is a severe zoonotic disease which affects both human and animals. The disease has a considerable economic and social impact, because it has numerous complications leading to important disabilities and even death. CE is a widespread chronic endemic helminthic disease caused by infection with metacestodes of tapeworm Echinococcus granulosus. This study was conducted to diagnosis human CE by hydatid cyst antigens from camels and sheep. Hydatid fluid and protoscoleces crude antigens corresponding to camel and sheep were resolute by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions and the protein bands of different antigens were exposed to infected patients serum CE through western blot (WB) assay. The camel hydatid fluid antigen revealed five polypeptide bands of 18-98.8 kDa by SDS-PAGE while sheep hydatid fluid antigen revealed four polypeptide bands of 20-100 kDa. Immune reactive bands were obtained through WB ranged from 25 to 125 kDa. The study showed prominent immune reactive bands of 92, 52.2 and 35.7 kDa which may helpful in diagnosis of human CE.
authors.affiliation: Department of Zoonotic Diseases, National Research Center, P.O. 12622, El-Tahrir Street, Dokki, Giza, Egypt.
authors.forename: Mohey Abdelhafez
authors.initials: MA
authors.lastname: Hassanain
authors.affiliation: Department of Zoonotic Diseases, National Research Center, P.O. 12622, El-Tahrir Street, Dokki, Giza, Egypt.
authors.forename: Raafat Mohamed
authors.initials: RM
authors.lastname: Shaapan
authors.affiliation: Department of Parasitology and Animal Diseases, National Research Center, Dokki, Giza, Egypt.
authors.forename: Fathia Abdelrazik M
authors.initials: FA
authors.lastname: Khalil
keywords: Camel; Cystic echinococcosis (CE); Human; SDS-PAGE; Sheep; Western blot (WB)