title: Nano-Sized Sunflower Polycations As Effective Gene Transfer Vehicles.
abstract: The architecture of polycations plays an important role in both gene transfection efficiency and cytotoxicity. In this work, a new polymer, sunflower poly(2-dimethyl amino)ethyl methacrylate) (pDMAEMA), is prepared by atom transfer radical polymerization and employed as nucleic acid carriers compared to linear pDMAEMA homopolymer and comb pDMAEMA. The sunflower pDMAEMAs show higher IC50 , greater buffering capacity, and stronger binding capacity toward plasmid DNA than their linear and comb counterparts. In vitro transfection studies demonstrate that sunflower pDMAEMAs exhibit high transfection efficiency as well as relatively low cytotoxicity in complete growth medium. In vivo gene delivery by intraventricular injection to the brain shows that sunflower polymer delivers plasmid DNA more effectively than comb polymer. This study provides a new insight into the relationship between polymeric architecture and gene delivery capability, and as well as a useful means to design potent vectors for successful gene delivery.
authors.affiliation: Department of Bioengineering and Molecular, Engineering and Sciences Institute, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Yilong
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Department of Chemistry, Lanzhou University, Lanzhou, 730000, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wei
authors.affiliation: Department of Bioengineering and Molecular, Engineering and Sciences Institute, University of Washington, Seattle, WA, 98195, USA.
authors.forename: James-Kevin Y
authors.initials: JK
authors.lastname: Tan
authors.affiliation: Department of Bioengineering and Molecular, Engineering and Sciences Institute, University of Washington, Seattle, WA, 98195, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Peeler
authors.affiliation: Department of Neurological Surgery, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Don O
authors.initials: DO
authors.lastname: Maris
authors.affiliation: Department of Bioengineering and Molecular, Engineering and Sciences Institute, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Drew L
authors.initials: DL
authors.lastname: Sellers
authors.affiliation: Department of Neurological Surgery, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Horner
authors.affiliation: Department of Bioengineering and Molecular, Engineering and Sciences Institute, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Suzie H
authors.initials: SH
authors.lastname: Pun
mesh_terms: D018014:Gene Transfer Techniques; D009682:Magnetic Resonance Spectroscopy; D008689:Methacrylates / Q000737:chemistry; D015394:Molecular Structure; D009696:Nucleic Acids / Q000737:chemistry; D009757:Nylons / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry*; D000071228:Polyelectrolytes; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D008689:Methacrylates; D009696:Nucleic Acids; D009757:Nylons; D011073:Polyamines; D000071228:Polyelectrolytes; C407037:poly(2-(dimethylamino)ethyl methacrylate)
keywords: nucleic acid delivery; polycations; polymeric architectures; sunflower polymers