title: Strong Correlation Between mRNA Expression Levels of HIF-2α, VEGFR1, VEGFR2 and MMP2 in Laryngeal Carcinoma.
abstract: The hypoxia that arises due to the rapid proliferation of tumor cells is a fundamental driving force for the canonical pathway of neovascularization. In the current study we report a very strong correlation between mRNA expression levels of HIF-2α (but not HIF-1α), VEGFR-1, VEGFR-2 and MMP2 in ex vivo samples from laryngeal carcinoma. Sixty-three samples from patients with histopathologically verified carcinoma of the larynx were examined in this study. Total RNA was isolated from both normal and tumor fresh frozen tissues of each patient and real-time quantitative PCR reactions were performed. The mRNA expression levels of HIF-1α, HIF-2α, VEGFR1, VEGFR2 and MMP2 were acquired. We found strong positive correlations between mRNA expression levels of HIF-2α and VEGFR-1, r s (98) = .671, p < .0005; HIF-2α and VEGFR-2, r s (98) = .742, p < .0005; HIF-2α and MMP2, r s (98) = .566, p < .0005; VEGFR-1 and VEGFR-2, r s (98) = .791, p < .0005; VEGFR-1 and MMP2, r s (98) = .709, p < .0005; VEGFR-2 and MMP2, r s (98) = .793, p < .0005. Our results provide evidence for the regulatory connection between HIF-2α and VEGFR-1, VEGFR-2 and MMP2 in the light of ETS1/ HIF-2α regulatory axis on a non-in-vitro level in carcinoma tissue, uncover some of the differences between the homologues HIF-1α and HIF-2α and round up and support the results from different experimental models in this field.
authors.affiliation: Department of ENT, Medical University of Sofia, blvd. "Han Pagan" 1, BG-1680, Sofia, Bulgaria. popov@todorpopov.com.
authors.forename: Todor M
authors.initials: TM
authors.lastname: Popov
authors.affiliation: Molecular Medicine Center, Medical University of Sofia, Sofia, Bulgaria.
authors.forename: G
authors.initials: G
authors.lastname: Stancheva
authors.affiliation: Molecular Medicine Center, Medical University of Sofia, Sofia, Bulgaria.
authors.forename: T E
authors.initials: TE
authors.lastname: Goranova
authors.affiliation: Department of ENT, Medical University of Sofia, blvd. "Han Pagan" 1, BG-1680, Sofia, Bulgaria.
authors.forename: J
authors.initials: J
authors.lastname: Rangachev
authors.affiliation: Department of ENT, Medical University of Sofia, blvd. "Han Pagan" 1, BG-1680, Sofia, Bulgaria.
authors.forename: D
authors.initials: D
authors.lastname: Konov
authors.affiliation: Department of ENT, Medical University of Sofia, blvd. "Han Pagan" 1, BG-1680, Sofia, Bulgaria.
authors.forename: S
authors.initials: S
authors.lastname: Todorov
authors.affiliation: Department of ENT, Medical University of Sofia, blvd. "Han Pagan" 1, BG-1680, Sofia, Bulgaria.
authors.forename: O
authors.initials: O
authors.lastname: Stoyanov
authors.affiliation: Molecular Medicine Center, Medical University of Sofia, Sofia, Bulgaria.
authors.forename: R P
authors.initials: RP
authors.lastname: Kaneva
authors.affiliation: Department of ENT, Medical University of Sofia, blvd. "Han Pagan" 1, BG-1680, Sofia, Bulgaria.
authors.forename: D
authors.initials: D
authors.lastname: Popova
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics*; D002277:Carcinoma / Q000235:genetics*; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics*; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics*; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000235:genetics*; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000235:genetics*; D000099162:Endothelial PAS Domain-Containing Protein 1
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D020778:Matrix Metalloproteinase 2; D012333:RNA, Messenger; D040281:Vascular Endothelial Growth Factor Receptor-1; D040301:Vascular Endothelial Growth Factor Receptor-2; D000099162:Endothelial PAS Domain-Containing Protein 1; C000588866:KDR protein, human; C522361:MMP2 protein, human
keywords: Flk-1; Flt-1; HIF-1α; HIF-2α; Laryngeal carcinoma; MMP2; VEGFR1; VEGFR2