title: Understanding the performance of sulfate reducing bacteria based packed bed reactor by growth kinetics study and microbial profiling.
abstract: A novel marine waste extract (MWE) as alternative nitrogen source was explored for the growth of sulfate reducing bacteria (SRB). Variation of sulfate and nitrogen (MWE) showed that SRB growth follows an uncompetitive inhibition model. The maximum specific growth rates (μmax) of 0.085 and 0.124 h(-1) and inhibition constants (Ki) of 56 and 4.6 g/L were observed under optimized sulfate and MWE concentrations, respectively. The kinetic data shows that MWE improves the microbial growth by 27%. The packed bed bioreactor (PBR) under optimized sulfate and MWE regime showed sulfate removal efficiency of 62-66% and metals removal efficiency of 66-75% on using mine wastewater. The microbial community analysis using DGGE showed dominance of SRB (87-89%). The study indicated the optimum dosing of sulfate and cheap organic nitrogen to promote the growth of SRB over other bacteria.
authors.affiliation: Department of Mining Engineering, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India.
authors.forename: Subhabrata
authors.initials: S
authors.lastname: Dev
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India.
authors.forename: Shantonu
authors.initials: S
authors.lastname: Roy
authors.affiliation: Department of Mining Engineering, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India; School of Environmental Science and Engineering, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India. Electronic address: jayantaism@gmail.com.
authors.forename: Jayanta
authors.initials: J
authors.lastname: Bhattacharya
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D003901:Desulfovibrio / Q000235:genetics / Q000378:metabolism; D004867:Equipment Design; D007700:Kinetics; D008670:Metals / Q000378:metabolism; D059013:Microbial Consortia / Q000235:genetics / Q000502:physiology*; D008906:Mining; D009584:Nitrogen / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism*; D014865:Waste Disposal, Fluid / Q000295:instrumentation* / Q000379:methods; D062065:Wastewater / Q000737:chemistry
chemical_list: D008670:Metals; D009584:Nitrogen; D013431:Sulfates; D062065:Wastewater
keywords: Growth kinetics; Microbial population analysis; Sulfate and metal removal; Sulfate and nitrogen source; Sulfate reducing bacteria