title: Expression of neutrophil gelatinase-associated lipocalin (NGAL) in peripheral nerve repair.
abstract: BACKGROUND
  Trauma is one of the causes of peripheral nerve injuries. Free radicals increase after tissue damage. Free radicals are usually scavenged and detoxiﬁed by antioxidants. In this study, we assessed the antioxidative role of the NGAL molecule in sciatic nerve repair in rats.
  
  
  METHODS
  The sciatic nerves of 40 rats were crushed and the total mRNA of samples from day 1 and 3 and week 1, 3, 5 post injury was extracted. The expression of the NGAL gene was confirmed by RT-PCR. For immunohistochemistry analysis, the samples were ﬁxed in paraformaldehyde and cut in 20 micrometer slices by cryostat.
  
  
  RESULTS
  The expression of NGAL signiﬁcantly upregulated in day 1, 3 and week 1 following the crushing of sciatic nerves in comparison with the intact nerves. Immunohistochemistry results also confirmed the protein expression of this gene.
  
  
  CONCLUSIONS
  The NGAL molecule showed upregulation in the degeneration process after nerve injury, so it may play an important role in nerve repair.
authors.affiliation: a Department of Physiology and Biophysics , Baqiyatallah University of Medical Sciences , Tehran , Iran .
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Aghanasir
authors.affiliation: b Department of Physiology , Tehran University of Medical Sciences , Tehran , Iran .
authors.forename: Hassan
authors.initials: H
authors.lastname: Aghaei
authors.affiliation: c Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences , Tehran , Iran .
authors.forename: Abbas Ali
authors.initials: AA
authors.lastname: Imani Fooladi
authors.affiliation: d Organ Anatomy Department, Graduate School of Medicine, Tohoku University , Sendai , Japan .
authors.forename: Majid
authors.initials: M
authors.lastname: Ebrahimi
authors.affiliation: e Department of Medical Biotechnology , Pasteur Institute of Iran , Tehran , Iran .
authors.forename: Ghasem
authors.initials: G
authors.lastname: Bagherpour
authors.affiliation: f Tissue Engineering Division, Baqiyatallah University of Medical Sciences , Tehran , Iran , and.|g Genomics Division, Systems Biology Institute, Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences , Tehran , Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Nourani
mesh_terms: D000209:Acute-Phase Proteins / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D016166:Free Radical Scavengers / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D000071068:Lipocalin-2; D054834:Lipocalins / Q000096:biosynthesis* / Q000235:genetics; D009416:Nerve Regeneration / Q000235:genetics*; D010525:Peripheral Nerves / Q000378:metabolism* / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D012584:Sciatic Nerve / Q000293:injuries / Q000378:metabolism* / Q000503:physiopathology
chemical_list: D000209:Acute-Phase Proteins; D016166:Free Radical Scavengers; C097278:Lcn2 protein, rat; D000071068:Lipocalin-2; D054834:Lipocalins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger
keywords: NGAL; peripheral nerve; repair