title: PTEN-mRNA engineered mesenchymal stem cell-mediated cytotoxic effects on U251 glioma cells.
abstract: Mesenchymal stem cells (MSCs) have been considered to have potential as ideal carriers for the delivery of anticancer agents since the capacity for tumor-oriented migration and integration was identified. In contrast to DNA-based vectors, mRNA synthesizedin vitromay be readily transfected and is mutagenesis-free. The present study was performed in order to investigate the effects of phosphatase and tensin homolog (PTEN) mRNA-engineered MSCs on human glioma U251 cells under indirect co-culture conditions. PTEN-bearing mRNA was generated byin vitrotranscription and was transfected into MSCs. The expression of PTEN in transfected MSCs was detected by immunoblotting, and the migration ability of MSCs following PTEN-bearing mRNA transfection was verified using Transwell co-cultures. The indirect co-culture was used to determine the effects of PTEN-engineered MSCs on the viability of U251 glioma cells by luminescence and fluorescence microscopy. The synthesized PTEN mRNA was expressed in MSCs, and the expression was highest at 24 h subsequent to transfection. An enhanced migration rate was observed in MSCs transfected with PTEN mRNA compared with non-transfected MSCs (P<0.05). A significant inhibition of U251 cells was observed when the cells were cultured with conditioned medium from PTEN mRNA-engineered MSCs (P<0.05). The results suggested that anticancer gene-bearing mRNA synthesizedin vitrois capable of being applied to a MSC-mediated anticancer strategy for the treatment of glioblastoma patients.
authors.affiliation: Hubei Key Laboratory of Embryonic Stem Cell Research, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.
authors.forename: Xing Rong
authors.initials: XR
authors.lastname: Guo
authors.affiliation: Department of Oncology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.
authors.forename: Qin Yong
authors.initials: QY
authors.lastname: Hu
authors.affiliation: Hubei Key Laboratory of Embryonic Stem Cell Research, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.
authors.forename: Ya Hong
authors.initials: YH
authors.lastname: Yuan
authors.affiliation: Hubei Key Laboratory of Embryonic Stem Cell Research, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.
authors.forename: Xiang Jun
authors.initials: XJ
authors.lastname: Tang
authors.affiliation: Hubei Key Laboratory of Embryonic Stem Cell Research, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.
authors.forename: Zhuo Shun
authors.initials: ZS
authors.lastname: Yang
authors.affiliation: Hubei Key Laboratory of Embryonic Stem Cell Research, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.
authors.forename: Dan Dan
authors.initials: DD
authors.lastname: Zou
authors.affiliation: College of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, P.R. China.
authors.forename: Liu Jiao
authors.initials: LJ
authors.lastname: Bian
authors.affiliation: Department of Surgery, University of British Columbia, Vancouver, BC V5Z 1L8, Canada.
authors.forename: Long Jun
authors.initials: LJ
authors.lastname: Dai
authors.affiliation: Hubei Key Laboratory of Embryonic Stem Cell Research, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.
authors.forename: Dong Sheng
authors.initials: DS
authors.lastname: Li
keywords: gene therapy; glioma cell; mesenchymal stem cells; phosphatase and tensin homolog; synthesized mRNA