title: Expansion microscopy with conventional antibodies and fluorescent proteins.
abstract: Expansion microscopy is a technique in which fluorophores on fixed specimens are linked to a swellable polymer that is physically expanded to enable super-resolution microscopy with ordinary microscopes. We have developed and characterized new methods for linking fluorophores to the polymer that now enable expansion microscopy with conventional fluorescently labeled antibodies and fluorescent proteins. Our methods simplify the procedure and expand the palette of compatible labels, allowing rapid dissemination of the technique.
authors.affiliation: Department of Chemistry, University of Washington, Seattle, Washington, USA.
authors.forename: Tyler J
authors.initials: TJ
authors.lastname: Chozinski
authors.affiliation: Department of Chemistry, University of Washington, Seattle, Washington, USA.
authors.forename: Aaron R
authors.initials: AR
authors.lastname: Halpern
authors.affiliation: Department of Biological Structure, University of Washington, Seattle, Washington, USA.
authors.forename: Haruhisa
authors.initials: H
authors.lastname: Okawa
authors.affiliation: Department of Chemistry, University of Washington, Seattle, Washington, USA.
authors.forename: Hyeon-Jin
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Chemistry, University of Washington, Seattle, Washington, USA.
authors.forename: Grant J
authors.initials: GJ
authors.lastname: Tremel
authors.affiliation: Department of Biological Structure, University of Washington, Seattle, Washington, USA.
authors.forename: Rachel O L
authors.initials: RO
authors.lastname: Wong
authors.affiliation: Department of Chemistry, University of Washington, Seattle, Washington, USA.|Department of Physiology and Biophysics, University of Washington, Seattle, Washington, USA.
authors.forename: Joshua C
authors.initials: JC
authors.lastname: Vaughan
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000648:ultrastructure; D002460:Cell Line; D007089:Image Enhancement / Q000379:methods*; D008164:Luminescent Proteins / Q000235:genetics; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D008164:Luminescent Proteins